LOCUS       DAOQKQ010000001        39389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_382_length_39389_cov_6.388061, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000001 DAOQKQ010000000
VERSION     DAOQKQ010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 39389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39389
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_382_length_39389_cov_6.388061"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..432)
                     /locus_tag="PKZ49_00005"
     CDS             complement(46..432)
                     /locus_tag="PKZ49_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HND34968.1"
                     /translation="MHTADIVLLIHFVFVLFVVASLPVIWLGTWFNWLFIRNFTFRLT
                     HLAAILFVVGESLIGITCPLTAWEHELRGTGSEQSFMQQWLHRILFYDFPEYVLTSIY
                     LLFALLVLITYKLAPPIAYKRNHNRH"
     gene            complement(447..986)
                     /locus_tag="PKZ49_00010"
     CDS             complement(447..986)
                     /locus_tag="PKZ49_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HND34969.1"
                     /translation="MSKFGKIVLYAVIAFSAMTAGFFFKAQQLTPQLSSDEKKRGVEK
                     FFSSGLPDVKGDMQPFSQWQGNVLLVNFWATWCAPCQEEIPEFIQAQETYRDKGLVLI
                     GIAVDQPDRVASFSRDFGINYPVVVGELDAFSLAEAMGNPQGALPFTVILNRAGEIVY
                     SHLGRIKIEEIEKIIKPLL"
     gene            complement(1027..2961)
                     /gene="dsbD"
                     /locus_tag="PKZ49_00015"
     CDS             complement(1027..2961)
                     /gene="dsbD"
                     /locus_tag="PKZ49_00015"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HND34970.1"
                     /translation="MRLFPFLFVLFFFTVNPAIAQEEGRSGVMTLLQKLGANFGQAEQ
                     ELLPPDEAFKLAIEVKDEHTLIAHFTPAKNYYLYRDKIAFEPQSEGIQIEKISLPPGK
                     MKDDLTFGKTEVYYQPFQAVIALKRGIEAPDQLSLASTYQGCNEPVGVCYAPIKKINE
                     VTLPAIKAAVNTVAETLSGNAAAAPIDSTAALFQTPSRPPAIETESFKIEQMFASGSF
                     GLILAGFFGIGLLLSFTPCVFPMFPILSGIIANRSQHMTKGRGFVLALAYVLGMAIAY
                     AIAGVAAGLSGAMLSAVLQNAWVLGTFALIFILLAFSMFGFYELQLPSFLQSKISEEA
                     GHLHGGHLSSVFGMGALSALIVSPCVAAPLAGALLYISQTRDIVLGGSALFVMALGMG
                     VPLLLIGASAGALLPKSGPWMESIKQFFGVLLLAVAIWLISPVVSTVIYMLLWAALLI
                     ISAIYLHAIDPLPVNALGFRKFLKGVGVIALLTGVALLIGVLSGSRDVLQPLSKLSIT
                     SNSSNVSFSEKGWDQLAHLPFQPVRSVTELDEIIAQSHDKYIMVDFYADWCISCKEME
                     RFTFSDERVQAKLKDVVLLQVDVTHGTPDDAALLKRFKLFGPPGILFLDRNGTEIPNI
                     KIIGYLNKDDFLAVLNAVLI"
     gene            complement(2994..3311)
                     /gene="cutA"
                     /locus_tag="PKZ49_00020"
     CDS             complement(2994..3311)
                     /gene="cutA"
                     /locus_tag="PKZ49_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HND34971.1"
                     /translation="MEAILILTSFPEEKGALRLAKQLIDQHKAACVHIGSQLTSIYHW
                     KGKTETATEVPVVIKTLSAHYAAVEQTIKALHPYELPEIIAVPIIKGLPAYLQWIADE
                     TVH"
     gene            3448..3657
                     /locus_tag="PKZ49_00025"
     CDS             3448..3657
                     /locus_tag="PKZ49_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND34972.1"
                     /translation="MIKRLFLLLQFLAFIAPIGIFLMYIIMDEGDQFTYEHYWVTAMS
                     FIPFVFVLLIKYMFSDLDQNKKDDR"
     gene            3654..4055
                     /locus_tag="PKZ49_00030"
     CDS             3654..4055
                     /locus_tag="PKZ49_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND34973.1"
                     /translation="MKKPILLIGLLILGIVEWVQISASSNTEHEIFSAEAVEKITIDA
                     GFGWGIFGGSVYNGNSDYTITQITVKLTPSQIIGTSSTSPLVAERYHIDITVPPLTKA
                     ALSMPLDADGTLEFLWELVQVQGYRVSVKSN"
     gene            4118..4360
                     /locus_tag="PKZ49_00035"
     CDS             4118..4360
                     /locus_tag="PKZ49_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND34974.1"
                     /translation="MIVRQNIVLIIVALFTFSNSAVATNQSAEEKNQTSLITDSAQQA
                     VQPSKHSILKEKKSEQPESTKPIKPNMADYCRKHTC"
     gene            4382..5158
                     /locus_tag="PKZ49_00040"
     CDS             4382..5158
                     /locus_tag="PKZ49_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HND34975.1"
                     /translation="MKLMTNLSNYILAASLTLSIANAAEWSYEEQKRWGSIEDHNQHE
                     VPHIYPYAVCSIGQHQSPVDLATSQVSKRLNNLKFRYYADKPDFFNTGHAVQVNLSND
                     YQGELLVGKEAYPLIQLHFHEPSEHVIGTKIFPAELHFVHVRGDGKIAVLGVLLEIGK
                     DNPVFQTILENTPHQGGELKQDSDVWLSITSLLPEKRDEYYTLAGSLTTPPCSEGVDW
                     YVLTDTTEISEAQLDRLKSFFSGNARLKQELNNRAIAKKK"
     gene            complement(5184..7724)
                     /locus_tag="PKZ49_00045"
     CDS             complement(5184..7724)
                     /locus_tag="PKZ49_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HND34976.1"
                     /translation="MNNKKIEKRVIQLYEKLELSNEIKLPEKDAAQLIRHGAHIIFQL
                     DTHNHFIYLNPAWETTTGLPVAKTVKLSLLNFVHEQDHDYLRKKLQLIRTSASKHSFH
                     TECRLKVNNTQIIHVEMQLIYSPENNKAQHVMGIMVDISKHKIFLENLILSNKRLSWA
                     LSGANDGYWDWNMETNYVYYSSRWKAMLGYSDRELLSPSLKTWEKLVNPEDKSRVLKL
                     VDDYVTGRKDKFETEFKMKHKGGHWVTVLSRASLARDNSGNLIYPKRLIGTHVDLTER
                     KQSELQLKIQNIALEAAANAIMITDTTATIEWINPAFTELTGYRRHEAVGMNVKDLMS
                     SGQQSELFYQDMWQTISSKKVWKGEIINKKKDGALYHEHMTITPVTNESDEICYYIAI
                     KEDISQRKASEKEILDLAFYDALTKLPNRRLFLDRLRHTVTACQRNKTYNALLFIDLD
                     NFKFLNDTHGHDAGDALLIEAAKRITSCIRETDTVARLGGDEFVVMLEQLGSDDSHAM
                     NITTQIAESILSRLHQEYRVNNIRHRGSSSIGICMFSDQYSKADDILKWADKAMYEAK
                     CAGRNTLRFFDPHMQQELERRTQLEKELWIAVDKHQFRLHYQLLINQSQQIVGAEALL
                     RWQHPDKGIVGPNEFLSLIENNGYIIPIGDWIIDEGIRQLQFWQQHDTLKSLTLSLNI
                     SAVQFQESGFVQKLKRKIEQSQIDHSRLILELTESSLINHIDDTVKKINELRTLGIRL
                     SIDDFGTGYSSLSYLKRFKLAQLKIERMFVRDILTDQDDLTLVKTIINMGKNLGLDVV
                     AEGVENQQQFEILKNAGCAKFQGYWINKPIPGEQITALVTSKHQQHVH"
     gene            8028..8339
                     /locus_tag="PKZ49_00050"
     CDS             8028..8339
                     /locus_tag="PKZ49_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND34977.1"
                     /translation="MEIKKWTDEELASTRNKLEEWCEKQKKSGKVMWIVTALLGAFAV
                     STGAVFIFFDGITLLSVVLIILGVMTCFTWYQSEKRRKNNIDFLEEVKREITHRAKRD
                     K"
     gene            8378..8911
                     /locus_tag="PKZ49_00055"
     CDS             8378..8911
                     /locus_tag="PKZ49_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND34978.1"
                     /translation="MNLLSWIKQIFSDQAGNQFGIRQKESKSLDFIFEQDDINCFKHL
                     CQFVWIQGEPIPLIFDLSNHVYVNQKITLPMIERLVAIGLVDYAPEGYVKKKLGKHTR
                     FFYHGRLTKIGFAQDAGNQLDLGVVLLTSSGKKLYATLSPIRNQQFYEYVIQHWFQKG
                     LLLSSPLGSPQRPHFVN"
     gene            complement(8904..9806)
                     /locus_tag="PKZ49_00060"
     CDS             complement(8904..9806)
                     /locus_tag="PKZ49_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HND34979.1"
                     /translation="MKNLLFDCNEFKVAHTGNVGFANLAQRPTNIKPIYSSKEHYQML
                     LLETQQEIGQLQEMLYAYNRYAILLIFQGMDSAGKGGVIKHVMSGVNPQGCQVFSFQQ
                     PSLEELDHDFLWRSNKCLPERGRIGIFDRSYYEEVIVVRVMPDLLKQQRLPLECADPK
                     KIWVERFQDIINLEHYLHRNGIQIIKFFLHLSKEEQRQRLIARIDEPSKNWKIGRADL
                     EAHKHWEAYQLAYDECIDATSTHHAPWYIIPADDKRNARLLVSHIIVETLQALKIAYP
                     ILSKDQLKELATIRQLLENEKPPN"
     gene            complement(9834..11687)
                     /gene="btuB"
                     /locus_tag="PKZ49_00065"
     CDS             complement(9834..11687)
                     /gene="btuB"
                     /locus_tag="PKZ49_00065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor"
                     /protein_id="HND34980.1"
                     /translation="MRSCLLFGFFVALCSTGYVSANNVQPPVIVTATRTAQTADETLA
                     SATVITQADIARLQARSVEDLLRGVPGLNIVNNGGPGKQTSFFLRGTESDHVLVLIDG
                     VRVGSVTSGTTSFENIPIQQIDRIEIVRGPRSSLYGSEAIGGVIQIFTRKGGGQIKPN
                     FSFGGGSYNSINGSAGLAGGGNQGWFNINVSGIGTRGFNACTGSETAGCFMTEASRVD
                     DQDKDGYRNLASSLRAGYRFNQAFEVDAHFLHSAGDTQFDGGYANRSKLKQQVLGGSI
                     RYSPFSFWQLNLTAGRSFEKLDTYKIERSEQIRRSTFLSQFDSIRDSVFLQNNFSIGN
                     HQLLTAGIDYFNDQVKSTESYLETSRRNWGLFAQHQAAIAAHNLQLSMRWDHNEQFGK
                     WVTGGIGWGYAITQNLRLTANFGNAFKAPNFNELYFPFSGNPNLRPESSRSFEMGITS
                     TTKLGKWSVNAYETHINNLITFDIQTFSSINVDRARIQGLESVFNTQILNWTINANFT
                     LLNPENASSPDDKILPRRAKHIFRIDADRQFNHYTLGGTFLAEGKRFDDTENTRLLNG
                     FVRVDLRAEYAFAQNWRLQARLENLFNEHYETAAFFNQPGRNVFFTLRYQT"
     regulatory      complement(11814..12013)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(12072..12755)
                     /locus_tag="PKZ49_00070"
     CDS             complement(12072..12755)
                     /locus_tag="PKZ49_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HND34981.1"
                     /translation="MLYLLLIILVIALIIGPAYWVKHVMEKYSHPDNRYAHTGAEFAR
                     QLLDQESLSHVKVELTESGDHYDPLNKVVRLTSDKFNNASLTAITVAAHEVGHAIQDR
                     DDYLALKLRTKLVVLVAPAQKLGAAILMLAPVSVIFIRSPLVGGLMLAGGFLSLGSAA
                     LVHIITLPMELNASFSRALPLLKKSKYLKEGDELHAHKLLTAAAWTYVSASLMTLLNM
                     ARWWAILRR"
     gene            complement(12887..13852)
                     /locus_tag="PKZ49_00075"
     CDS             complement(12887..13852)
                     /locus_tag="PKZ49_00075"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HND34982.1"
                     /translation="MSNNAVESVEFKFRIGEITLWTSHLKLNVFRNHFTEYEHPYTEE
                     SDYSDSIDEKLDGVLFRSRPITTLLPRFTLKQDVIRYVPKQYHRYFIDLTGSKEEYLE
                     KFSAKSRSTLKRKIKKFSDHCGNQIDWREYKTLAEMEIFYPLARQVSTKTYQERMLAA
                     GLPEDDLFIQRMYALAQKDSVRAYLLFCQDKPVAYLYCPISNGILFYQYLGYIPEMAQ
                     WSPGTVLQWVVIENLFLESHFAMFDFTEGPGKGSGGHKEFFSTNSTLCADIFFLRKTN
                     INLFLVLLHTKIDSFSAKIGIFLDRFGLKTKIKNFLRKLYPSASH"
     gene            complement(13861..14856)
                     /locus_tag="PKZ49_00080"
     CDS             complement(13861..14856)
                     /locus_tag="PKZ49_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HND34983.1"
                     /translation="MPAKQNIDHSKSPLNVIISVDVELWPDNWDLGHTEFNRCLNKFI
                     HGKTTSGNYGLGFQLAQLKEHNLKCVFFVESLFSGHYGIGPLQEIVAQIQEAGQEVQL
                     HLHTEWLGRGRFHNPLNKTGYNLFNFNQAEQQLLICQGKEFLAQAGADNLLAFRAGNF
                     GSNLQTLEAVPQAGFIFDSSINPRFYIKNGLDTPLHVEEYKEGIYEFPLTIFKEWGGR
                     LSQFQFGGSCSFKEMANLIRQAWINEWHSVVILSHGSELLNRAKTRPDKIVVDRFVQT
                     CRFLANNRDLFKTIWFSDIQPETIYAKSKESCVLQSGFINTAHRYLEQTTRRLYG"
     gene            15138..15971
                     /locus_tag="PKZ49_00085"
     CDS             15138..15971
                     /locus_tag="PKZ49_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HND34984.1"
                     /translation="MKTSLFDLAASCLIANEIEEKLLLTQQATQAWRNKLLSLESHEP
                     MRLINQPGQPAKLVVVKPSDVPRRRLGTKLGLIALIHAIAHIEFNAINLAWDAIHRFR
                     GLPDSFYDDWVTVASEEAYHFQLLRKRLKELDHDYGDLPAHNGLWDMALLTASDPLIR
                     MALVPRVLEARGLDVTPGIIKRLRQIGDEKTIEILEIILCDEIGHVSIGSRWYNFFCN
                     QRQIDPEKKFSELVNQYFGGQITGPFHYEARQKAGFTLNELSMLEGMEIPISRSNNCN
                     F"
     gene            16085..18685
                     /gene="clpB"
                     /locus_tag="PKZ49_00090"
     CDS             16085..18685
                     /gene="clpB"
                     /locus_tag="PKZ49_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HND34985.1"
                     /translation="MRLDKFTTKFQQALGDAQSIALGKDHPYIEPQHLLLALLQQEDG
                     STVSLLQRAGINVNPLREGLAKNLERMPKIEGGGGEINISRELNNLLNLTDKEAQKHG
                     DQFIASEMFLLALLEDKGETGLLLKQHGASRSALEQAIKSVRGGEQVSSAEAEGGREA
                     LKKYTLDLTERARAGKLDPVIGRDDEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPDTLKNKRVLSLDMAALLAGAKYRGEFEERLKAVLKELSQDEGRTIMF
                     IDELHTMVGAGKAEGAIDAGNMLKPALARGELHCVGATTLDEYRKYVEKDAALERRFQ
                     KVLVDEPTVEATIAILRGLQERYELHHGVDITDPAIVAAAELSHRYITDRFLPDKAID
                     LIDEAAARIRMERDSKPEVMDKLDRRLIQLKIEREAMKKEKDEASQKRLALLEDEIKK
                     LEREYADLEEILKSEKMRAQGSQQIKEELEKLRKEVEVATRKGDWQKVSELQYGRIPK
                     LEAQLAEQVQHAEATLPERELNKPKLFRTQVGAEEIAEVVSRATGIPVAKMMQGEREK
                     LLLMEQKLHERVVGQDEATRLISDAIRRSRSGLSDPNRPYGSFLFLGPTGVGKTELCK
                     ALASFLFDSEDHLIRIDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEMVRRKPYSVI
                     LLDEVEKAHPDVFNVLLQVLDDGRMTDGQGRTVDFKNTVIVMTSNLGSQMIQQMSGDD
                     YQIIKLAVMGEVKTYFRPEFINRIDEVIVFHALDAKHIQSIAKIQLRNLENRLAKMEI
                     HLEISEAALAKIAEAGFDPVFGARPLKRAIQTQIENPLAKELLEGHFAAKDTIHVDCE
                     DETITFTKSS"
     gene            18746..19624
                     /gene="dapA"
                     /locus_tag="PKZ49_00095"
     CDS             18746..19624
                     /gene="dapA"
                     /locus_tag="PKZ49_00095"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HND34986.1"
                     /translation="MFKGSLVAIVTPMHDDGALDLEKFCELIDFHIAEKSDGIVVVGT
                     TGESPTVDFEEHHLLIKTAVSHAAGRIPIIAGTGANSTREAIELTQFAKNAGAAACLS
                     VVPYYNKPTQEGMYQHFKTIAEAVDIPMILYNVPGRTVADISNQTVVKLASVPNIIGI
                     KDATGNIGRGCDLLSQIPENFSVYSGDDATSLALLLLGAHGVISVTANVAPKLMHEMC
                     AAAFSSDLANARAINDKLLKLHTDLFVEANPIPVKWAVSRMGLMGNGIRLPLTPLSDQ
                     YHSLLIAAMKQAEVLN"
     gene            19645..20781
                     /gene="bamC"
                     /locus_tag="PKZ49_00100"
     CDS             19645..20781
                     /gene="bamC"
                     /locus_tag="PKZ49_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HND34987.1"
                     /translation="MKRFSKLFGLLLAIIMLISGCHLLPESQKMEYKSAGKLPPLEIP
                     PDLNAPQADDRYAVPDMASTGSATYSSYNRDRDEQPRSQGSYVLPVAKDTARIARSGT
                     QRWLVVQLAPEDAWPVVKEFWQDMGFLLKVEAPDVGIMETDWAENRAKIPQDLIRGLL
                     STFLDSIYSSAERDKFRTRFERSESGSTEIYISHRGMDEVFVDRSTNRTAWQPRSADP
                     NLEAEMLTRLMMRFGVEEESARKEVAANENIITQDQAYLDKTRHGVLIVSETFDRAWR
                     RIGLALDRGGFTVEDRDRSNGVYFVRYVSPEYESRKTSDDKGLLTKLAFWQKSQDDSR
                     TEKFRIQVDGVGASSEVKVLTLEGAPEESAAADRILKLLYEQLK"
     gene            21187..23049
                     /locus_tag="PKZ49_00105"
     CDS             21187..23049
                     /locus_tag="PKZ49_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HND34988.1"
                     /translation="MLDIQHLTYLQNGLPLLSDVNFQVFANQRVGLVGKNGSGKSTLF
                     RLIRGEIHPEKGEVNLQVGKTLAYVEQEIINSAKPSLEFVLDGDSELRQLEAILKQDN
                     HDAKWFDAQQRYELIDGYGARARAAQLLDGLGFSNTEHDAPVSKFSGGWRMRLNLARA
                     LMHRADLLLLDEPTNHLDLEAIFWLEHYLQNYPGSLIIISHDREFLNVCVNRIAHIEH
                     QKIQSYSGNYDDFERARMERIAQHEQTYEQQQKQIAHMEDFVRRFRAKATKAKQAQSR
                     IKALERLTRVAPIQVENGYFSLQILAPDNGPDTLLKIDSMGFGYTDESMLFQKVNLCL
                     RTGDRIVLLGPNGVGKSTLIKLLTAELLPAKGKVEINSSIRIGYFSQHQLEYLNGAQT
                     PLQHLQQLAHNETTLTLRNFLGRFGLADDSKDRPIATFSGGEKSRLALALLAWQKPHL
                     LLLDEPTNHLDLDMRDALIVALENYTGSLVIVSHDRSLIRAVADELWVVADSNVHLFD
                     GDLDDYKDWLEKRRDNGTDKAKPKKQSQTKRATANKRSMLTQQTKIEAALAGVQQELA
                     ALNLQLSDPAIYANASREAISELNLKRSELEKKIDALEANWLELESLMEEFGKV"
     gene            23148..24215
                     /locus_tag="PKZ49_00110"
     CDS             23148..24215
                     /locus_tag="PKZ49_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND34989.1"
                     /translation="MKTKFFILKLITISLILNFPINAANAMPGMHGSEGKSHTEAPRV
                     SKSMGDKINTTGAELEITYSADGKTAIFVSTREGSIESPGSPYNFDIWISKNVDGEWQ
                     TPIHLGPDIDPTLGPNINTSAWELEPSFSDDGNVIYFTRYEPGNLLSGDLYVVQKIDG
                     VWQTAKNWNDVPELPNINTPTGEEHCPIIASDSLIYFSYHQPGVTQDSDIWKVEKKDG
                     VWQQPESLGAKINSPHRDHMHWTGLSKDGKSLIITSMRPDLGSRGGHDEWISHQDEKG
                     EWQVPVNLGDAVNTAGEDMCWTFTPDGKKFTGSWGPYSTYDMDIRWINKDDVPLLKNF
                     EPIGPPPNLLINRKSTQNTSQ"
     gene            complement(24262..24555)
                     /locus_tag="PKZ49_00115"
     CDS             complement(24262..24555)
                     /locus_tag="PKZ49_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND34990.1"
                     /translation="MGRNHFDKELFELDERIGRLAIACGVDIAQKNTIVSLIKGNYSV
                     CLQGDNPRRQELRGLLMLKYHIEESCIDAIGANECVQLIDDEEARLRQRGFSI"
     gene            complement(24635..25249)
                     /locus_tag="PKZ49_00120"
     CDS             complement(24635..25249)
                     /locus_tag="PKZ49_00120"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HND34991.1"
                     /translation="MPNNKSVIFAVVSSFFSFALISLSTPLKANDNLHAQSILVAEAH
                     QHDHKSNAQEKENNQPNSSHQHGQDKKNKHQHGMMEEKHDYAHMIIFHADKLKLTNEQ
                     LGKLVRLHLKHEEAHKKIKEKLKDSMHAFKKAKMDPSSTDEQLSSLGKDHANAFNAMV
                     EHHIQERRAIHNVLSAEQKNQLISTIKMNHSAHDDKGGGHSHDH"
     gene            25359..25541
                     /locus_tag="PKZ49_00125"
     CDS             25359..25541
                     /locus_tag="PKZ49_00125"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND34992.1"
                     /translation="MYPKAQIPCIQLLLLRNLDPEKHIALGKALASLREKNILIIGSG
                     VISSFENIFYPRYRQQ"
     gene            25504..25788
                     /locus_tag="PKZ49_00130"
     CDS             25504..25788
                     /locus_tag="PKZ49_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND34993.1"
                     /translation="MKTFFTHDIDSSRENDAFDQWLIQMYACPEILSEVREQRLIEWE
                     KAPFARFCHPREEHLLPLQVCYGIACADSPIAKVMFNEQIMGKKVTSLLW"
     gene            complement(25817..26854)
                     /locus_tag="PKZ49_00135"
     CDS             complement(25817..26854)
                     /locus_tag="PKZ49_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HND34994.1"
                     /translation="MAQEKITNIQLPGVDDRPLWDVIFAIYGYPALLLAHRLKIFSTL
                     AKNTCTLSEICNALNLKPRPVEAILTTATALGFLSLHEERYRLTPVTEVYLLENSPNY
                     FGFFWDMMIDNYQASSIASLEEAILTDKPQVYGGNNLFKTHEEQIELLRRFTAGMHSI
                     SITSALVWPTIMDFSRHRIMLDIGGGSGAHSIGAAIKLPNLEQALIMDFPQVCEVAEG
                     YIAQYKMQNRVKVHAANIWQDHWPMADLHFYSNMYHDWMPDKCHFLTAKSFGNLESGG
                     RIVIHEMLYNDDKTGPFAIAAFSMIMMGWTEGKMYSGQELTKMLDEVGFEDIQIYKAF
                     GYHSIVTGVKP"
     gene            complement(26885..27385)
                     /locus_tag="PKZ49_00140"
     CDS             complement(26885..27385)
                     /locus_tag="PKZ49_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HND34995.1"
                     /translation="MFNLGSMLNLGSTEPVIGSASTIVECSASELFKYIGENLFQNYP
                     KWSPEVKELEKITAGPVQFGTIGRQVRVDQGRRSESRFKISDFTPEKRLTFSGVSDPY
                     RCSYELQAGDLDNSTKLIFTFELLELLVIMRPFEGLVRASIKDGVTRTVQNIKRLVET
                     EKLKHE"
     gene            27390..27557
                     /locus_tag="PKZ49_00145"
     CDS             27390..27557
                     /locus_tag="PKZ49_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND34996.1"
                     /translation="MSPPFCQNNGHSLAMENFMHAFSLHHIYDVNHLYFIVNARRDLF
                     YFLQDESAHHI"
     gene            27895..29142
                     /locus_tag="PKZ49_00150"
     CDS             27895..29142
                     /locus_tag="PKZ49_00150"
                     /inference="COORDINATES: protein motif:HMM:NF013715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HND34997.1"
                     /translation="MSLKEKLSQFGPGLLYAGAAVGVSHLVQSTRAGAEFGFQLVAIV
                     ILVNILKYPIFELGPRYAAATGKTLLDGYHQIGAWAVWIYFLLSALTMFIVMGAISIV
                     TAGLFAQLTGMPLDAPIIAMLLLVLSVQILGFGHYSLLDRSMKFIMIVLTISTLLALI
                     FSFFAPIEKQAEFETTFNFLESAHILFLVALIGWMPAPIDISIWHSVWSVSKNKEQGH
                     RIPLAQALLDFKVGYWGTMVLAVCFLTLGALVMYGTPESPASNSTEFAKQFIGFYTTS
                     LGEWAFPIIALAAFATMFSTLLTCLDAYPRTLRRSTELLFPRLDSLPYHNKIYWYWLI
                     LTALGTIVLLHFFLRDMRAMVDLATTVSFVLAPVLAILNYMALKRSEVPAHAKLTGAS
                     QYAYLASTGMLIAFSLWFLAYKP"
     gene            complement(29167..30300)
                     /locus_tag="PKZ49_00155"
     CDS             complement(29167..30300)
                     /locus_tag="PKZ49_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HND34998.1"
                     /translation="MQLVYLSPLPWDSFSQRPHQFVEWFHNKHGGEVLWIDPYPTRLP
                     NLGDFRRIKSSKFLSKNVIVGNGNKPPWLTVIQPFVPPIEPLPGSCYVYRWLWNDILQ
                     AIDRFIESQNETLLGIGKPSELALQVLFRYPSLKSIYDAMDDFPAFYHGISRFAMMNR
                     EQRIAKQVTRISASSTKLTERFIEYQSKLTLVHNACAVDALPSINDTLKTRATEKQVL
                     GYLGTIGDWFDWAFVAKLAETVSTSTIHLIGPIFTKIPASLPQNIKFLPACEHSAAIQ
                     AMLSFTVGLIPFKRNDLTAAIDPIKYYEYRSLGIPILSTHFGEMSLRQGQPGIFIANA
                     SCDLGDHINKAMDYKYPKSEINWFRKINSWEARFNSGNILPQT"
     gene            complement(30300..31409)
                     /gene="wecB"
                     /locus_tag="PKZ49_00160"
     CDS             complement(30300..31409)
                     /gene="wecB"
                     /locus_tag="PKZ49_00160"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HND34999.1"
                     /translation="MSIGINRTILCVIGTRPEAIKMAPVILELRKKSFQVRVLATAQH
                     RNLLDQVLDLFGIVPNIDLNIMRPNQSLAPLTARLLLSAEEVLASEKPDAVLVQGDTT
                     TVMAVALACFYQHIPIGHVEAGLRTWDMDNPFPEEANRVITGKLARWHFAPTAESRLN
                     LMRDGISDNNIFITGNTVIDALLSLADQNLALPINLDPDKRLVLITAHRRENFGEPLR
                     SICQAIKSLAQNNPGVEFLYPVHPNPNVTETVNELLASQKNIHLCAPLDYAHFVAVMK
                     RSFLILTDSGGIQEEAPALSKPVLVLRNETERPEAVTQGVAKLVGHDYDRILSETQRL
                     LDDELAYFAMRKGISPYGDGHAAERIVKVLEERLP"
     gene            complement(31393..34503)
                     /locus_tag="PKZ49_00165"
     CDS             complement(31393..34503)
                     /locus_tag="PKZ49_00165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HND35000.1"
                     /translation="MDSINSNAYWNNRFKSNWDSFEGPAQSRFFSQIAIEHLPKWFIE
                     QIKRNNLTFADWGCAQGDGSDVWASYIDSSQITGIDFSLAAIEKATRRYPAICFLNED
                     WLSKSLISNEIVYDIIFSSNTLEHFHKPYEVLTSILKRARKAVVLALPYKEIDRIDEH
                     FFSFLPENIPLMLKNNFRLVVSRVIDCKTRPRTAWQGYQIILLYAHSDWIDQLLLSLN
                     DSRIESFDADSHIENLNNQIKRLNDEITHQTEQIVQLKQTILEQNQHITHYQQTLSEY
                     NLKQQQFYQVIAERDTHISNLEQTLTQRDDQINQLEIKRLLEYEKTIQLSDWAQKIDN
                     RPFHYGTKKLVLKSAKNFYHALPISHRYKQKFKFFLSQIPYLKKIIKTFPVSVNQISR
                     SALDHKPLVPDNLRALINGEKLNSRSDYLIFSVIDWHFRLQRPQHLARSLAKLGKRVF
                     YFSNHFIDSPTPGYAIERLDSDLELYQIKLYVEDAPAIYFSSPTVKHLNNLQLSITNF
                     FIDFATLSSVSIIQHAYWYPLAIGLPNSLRIYDCMDHHEGFGNVSEDLIKLEKEMMCK
                     FDQIIVSSAWLEKQAQQYNNNVVIIRNASEYEHFNNPPIEIYLDPKGRKIIGYYGAIA
                     EWFDLELVKNLALAEPNALILLIGNDTINASHISKAIPNIIFIGEVSYNRLPFYLYAF
                     DVCLLPFKISPLTLATNPVKIYEYLASGKPVVSVDLPEISQFENLVHRAKTSDEFISL
                     VSTCLKSSSPEENQTELRKQFACKQTWNHRVNDLVQAIQQIPMPKVSVIVLTYNNLNL
                     TKLCLNSLLQWSDYPNLEIIIVDNNSSDNTPDYLTELNTRHPTIQVIRNKTNEGFAKG
                     NNIGLIAATGDYLVMLNNDTVVTPGWILTMLRHLQADPSIGIIGAVTNNIGNEAKIDI
                     TYPLLSEEMLPRILSHTIPHMGKNFKLRTAAFFCVMMSRNVFQQVGLLDENYGRGFFE
                     DDDYCRRIDRLDLQIACAEDVFIHHHLSASFNQIPDRDKQALFEKNKAYYESKWGKWN
                     PHEYRH"
     gene            complement(34511..35881)
                     /locus_tag="PKZ49_00170"
     CDS             complement(34511..35881)
                     /locus_tag="PKZ49_00170"
                     /inference="COORDINATES: protein motif:HMM:NF025877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HND35001.1"
                     /translation="MSSEIALQTDGLGKCFHIYDKPRDRLLQMFGASKKKFFREFWAL
                     RDISFTISKGEIIGVIGTNGSGKSTLLQIIAGTLSPTIGTVNVIGRVSAILELGAGFN
                     TEFTGRENVLLNAAIIGFSRDAIAEQMEKIRAFSELGDFFDQPIKTYSSGMQARLAFS
                     IAIHVDPEILIVDEALAVGDARFVAKCMRRIKDIMSKGTTVLFVSHDISSVRTLCSRT
                     LWLDQGYLVEDGDVFPVTGRYMAYMFNNDQPSIEASNLAHKNIARHKTKSTKLSELPI
                     ENQADSSLQGEITGNGQTSPPDSRPEIALDSKPITHWGSYKGIILSASVRDSHGVRKN
                     TLHWGAPIEIFIDLRIPPEISRDHLSIAFSIKDLKGTDLIVSTTHDFELKRLPEDERF
                     SISFKLTNPLVTGKYLLVAAVENRQYRDIHYYEYFEGAHYFASLSKERFFGLFQPSIE
                     QAVSLK"
     gene            complement(35871..36698)
                     /locus_tag="PKZ49_00175"
     CDS             complement(35871..36698)
                     /locus_tag="PKZ49_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HND35002.1"
                     /translation="MNPHVAPPTSPFYLLKSLRAHGSLIFNLIKREIIGRYRGSIMGI
                     LWSFFNPVLMLIVYTFVFSIVFKARWIGGTDSKTEFALVLFAGLMMYNLFAECINRAP
                     GLVLGNVNYVKKVVFPLEILPIVAIGSATFHLIISLFVWLCFYLIFIGIPPSTIGLLP
                     VLLFPFFLMILGLTWLLASLGVYLRDVTQIIGILTTALLFLSPIFYPISALPEEYHYF
                     IQVSPLTFIVEQAREIMIWGKELNWSAWIIYSLFSIVTAWLGFAWFQKTRKGFADVL"
     gene            36933..37703
                     /gene="xth"
                     /locus_tag="PKZ49_00180"
     CDS             36933..37703
                     /gene="xth"
                     /locus_tag="PKZ49_00180"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HND35003.1"
                     /translation="MKIGTWNVNSLKVRLPQVIQWLEVNQPDVLCLQETKLQDSFFPL
                     TELSASGYSSIYVGQKTYNGVALLSKQPGCDVITTIPNFEDDQKRVVAATYGDIRVVC
                     IYVPNGESIDSEKFQYKLNWLSALNRWLHEELTQFPKLVLLGDFNIAPADSDVHNPEL
                     WKDQVLCSSPERDAFQNLLNLGLYDSFRLFEQPGKSYTWWDYRMLAFRLNKGLRIDHI
                     LLSKALTESCESCVIDKTPRKLERPSDHTPVVVTLKIG"
     gene            complement(37758..38210)
                     /locus_tag="PKZ49_00185"
     CDS             complement(37758..38210)
                     /locus_tag="PKZ49_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35004.1"
                     /translation="MMKTVLLFAILFAHSICFAEPSIYKHFGDRGETIYSDHHLKGAE
                     QAELPVLTVLPTSQRLSKVSPSIAQMEADESLQHNETEQIGTHQSRVIPSAKSEKVMQ
                     PTEISANQTDEVELSPEEQLQLIIDDMELYDDDIDRLELELYKIGADY"
     gene            complement(38226..38684)
                     /locus_tag="PKZ49_00190"
     CDS             complement(38226..38684)
                     /locus_tag="PKZ49_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35005.1"
                     /translation="MKQIISITLILLAIFTITAQVSASSNIHKTVDNKGRVTYANAQG
                     KNYAKATHASLSSGRIYPKIDNHQQKKRDFDRRQILENELAIETELLVGIINSLRLIS
                     IKMKEIQSESSQPVSAAINDFENDNINTLLNLASVHERNIIALRKELNSL"
     gene            complement(38814..>39389)
                     /gene="glnA"
                     /locus_tag="PKZ49_00195"
     CDS             complement(38814..>39389)
                     /gene="glnA"
                     /locus_tag="PKZ49_00195"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111135.1"
                     /note="forms a homododecamer; forms glutamine from ammonia
                     and glutamate with the conversion of ATP to ADP and
                     phosphate; also functions in the assimilation of ammonia;
                     highly regulated protein controlled by the
                     addition/removal of adenylyl groups by adenylyltransferase
                     from specific tyrosine residues; addition of adenylyl
                     groups results in inactivation of the enzyme; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HND35006.1"
                     /translation="GKNLFSGNSYAGMSEMALFYIGGIIKHTKALNAITNPGTNSYKR
                     LVSGYEAPVNIAYSASNRSAAIRIPHASNPKQRRIEIRFPDPLANPYLAFSALLMAGL
                     DGIQNKIHPGDPMDKNLYDLPPEEASKVPATCNALEEALEHLDKDREFLTRGGVFSND
                     MIDAYIALKMEEVILYRTAPHPIEFSMYYSL"
BASE COUNT        11694 a         7608 c         8273 g        11814 t
ORIGIN      
        1 atcaggttgc ctcgacacag gataatcata acggttttaa ataaactaat ggcgattatg
       61 gtttctttta taagcaatgg gtggcgcaag cttataagtt atcaagacga gtagtgcaaa
      121 taacaggtaa atactggtta acacatattc tggaaaatca taaaataaaa tccgatgcaa
      181 ccactgttgc ataaaacttt gctctgatcc tgttccgcgt aattcatgct cccatgctgt
      241 taatgggcag gtaataccga tcagagattc accgacgaca aacaagatgg cggcgaggtg
      301 tgttaaccta aaagtgaagt ttctgataaa aagccagttg aaccaagtac ctagccaaat
      361 aaccggcagg ctagctacta cgaataaaac aaaaacaaaa tgaattaaca gaacgatgtc
      421 agcagtgtgc acgtgctgga tgattattaa agtaacggtt taatgatttt ttcaatttct
      481 tcgattttga tgcgacctaa atgactataa acaatttcac ccgcacggtt taagataaca
      541 gtaaatggga gtgctccctg gggattcccc attgcctcgg ccaacgaaaa tgcatcaagc
      601 tctcctacga cgacagggta attaattcca aaatcgcgac tgaaggaggc aactcgatcc
      661 ggttgatcta cagcgatccc aattaaaaca agccctttat cgcgatatgt ttcttgggct
      721 tgtataaact ccggaatttc ttcctggcac ggtgcacacc aggttgccca aaagtttacc
      781 agcaacacat ttccctgcca ttgtgaaaac ggctgcatat cccctttaac atcaggaaga
      841 ccggaagaaa agaacttttc tacccccctt ttcttttcat cgctacttag ttggggtgtt
      901 agttgttgtg ccttgaaaaa aaaaccagca gtcattgcac tgaaggcaat aacagcatac
      961 agcacgattt tgccaaattt tgacataagc ttttatcctt gagaaaactg agaatgcgct
     1021 attaaattag attaataccg cgtttaaaac cgctagaaaa tcatctttat tgaggtagcc
     1081 gataattttg atattgggta tttctgtacc gttacgatct aaaaataaaa ttcccggtgg
     1141 gccaaatagc ttaaagcgtt taagcaaagc ggcatcatcc ggcgttccat gggtaacatc
     1201 gacttgcagc agtactacat cttttaattt tgcttgtact cgctcatcag aaaaggtgaa
     1261 tcgctccatt tctttacagg agatgcacca atccgcgtaa aaatcaacca tgatgtattt
     1321 atcatgagac tgtgcaatga tttcgtctag ctcagtaact gatctgaccg gttggaatgg
     1381 taagtgggct agttgatccc atcccttttc agagaaacta acattgctgg agttacttgt
     1441 aatgctcaat tttgagagcg gttgcaaaac atctcggctt ccggatagga ctccaatcag
     1501 taatgcaaca cctgtcaata atgcgatgac accaacacct ttgagaaatt tacggaaacc
     1561 caaggcatta acggggagcg gatcaattgc atgcagataa attgccgaaa tgatgagcaa
     1621 tgcagcccat agcagcatgt aaattaccgt ggagacaaca ggtgaaatta accaaatcgc
     1681 gacagccagc aatagaacac caaaaaattg cttaatcgat tccatccaag ggccagattt
     1741 gggaagtaat gcgccggctg atgctccgat taataagaga ggaacgccca ttccaagcgc
     1801 catgacaaat aaagctgacc cccctaggac aatatcacgc gtctggctaa tatagagcaa
     1861 agcgcctgcg agcggtgctg caacacaagg actgactatc aaagcggata atgcacccat
     1921 tccaaaaaca ctggatagat gaccaccatg gagatggccg gcttcttctg aaattttgct
     1981 ctgtaagaag ctgggaagct gtaattcata aaaaccaaac attgaaaaag cgagtaagat
     2041 aaatatcagg gcaaaggtgc ccaataccca ggcattttgt aacactgcag aaagcattgc
     2101 gcccgacaaa ccagccgcca caccggcaat tgcataagca atagccattc ctagcacgta
     2161 agcaagggcg agcacgaaac ctcggccttt agtcatatgc tggcttcgat tggcgatgat
     2221 cccagataag atggggaaca ttggaaatac gcagggtgta aaagaaagta agagaccaat
     2281 accaaagaag ccggccagta ttaggccaaa actgccactg gcaaacattt gttcaatctt
     2341 gaaagattct gtctcgattg cgggtggacg agaaggagtt tgaaataacg cagcagtact
     2401 atcaatgggt gccgccgcgg cattcccact caatgtctca gcaacagtat ttacggctgc
     2461 tttgattgca ggtagcgtta cctcatttat ttttttgatc ggcgcgtagc agacaccaac
     2521 gggttcatta caaccttgat aggttgatgc cagtgaaagt tgatctggcg cctcaattcc
     2581 tcgctttaat gcgatcactg cctgaaaagg ctgataataa acttctgttt taccaaacgt
     2641 gagatcatct ttcattttac caggcggcaa tgaaattttt tcaatctgaa ttccttcact
     2701 ttggggttcg aatgcgattt tatcgcgata gaggtaataa ttcttggctg gcgtaaaatg
     2761 agcgattagc gtgtgctcgt cttttacctc aatagcgagt ttaaatgctt cgtctggcgg
     2821 tagaagttct tgttctgctt gcccaaaatt agcccccaat ttttgtaaaa gggtcatgac
     2881 acccgagcga ccctcctctt gagcaatcgc cggattaacc gtaaaaaaga aaagtacaaa
     2941 taaaaatgga aataaacgca taacttaatt aattagtaag taataaatca attttaatga
     3001 acggtttcat cagcaatcca ttgtagataa gcaggcaacc cttttataat agggaccgct
     3061 atgatttctg ggagttcata gggatgcaag gctttaatcg tctgctcaac cgctgcatag
     3121 tgcgcggata atgttttgat tactaccggc acctctgttg ctgtttcggt tttacccttc
     3181 caatgatata ttgaagtcaa ttgcgaccca atatgaacac atgcagcttt atgttgatcg
     3241 atcagttgct tagctagcct taaagcccct ttttcttcgg gaaagctggt aagtatcagt
     3301 attgcttcca taatatctgt tttgtacacc gagaaaacta gattttttca tttttttagt
     3361 attaaactca agctttcttt taataacata agatcagcta gatcttctag ccattgaaaa
     3421 acgaattgtt ttaaacgtgg gttcttaatg atcaaaagac tatttttatt attgcagttt
     3481 ctcgccttca ttgcgccgat tggtattttt ctaatgtata tcatcatgga tgaaggagat
     3541 caatttacct atgagcatta ctgggtaacc gcgatgagtt ttatcccctt tgttttcgtg
     3601 ttgctgatta aatatatgtt ttcagattta gaccagaata aaaaggatga cagatgaaaa
     3661 agcctatttt attgattgga ttattgatat tagggattgt tgaatgggtt caaatttctg
     3721 caagctcaaa taccgaacat gaaatatttt cagctgaagc agtagaaaaa attacgattg
     3781 atgccggttt tggttggggg atttttggtg ggagtgttta taatggtaat tcggattaca
     3841 cgataactca gattaccgtt aaattgacgc catcccaaat tattgggaca tcaagcacat
     3901 cgccattagt agcagagcga tatcatatcg atattacagt ccctccttta acgaaagctg
     3961 cgctttcaat gccactcgat gcggatggca cactagagtt tctgtgggag ttggtacaag
     4021 ttcaaggtta tagggtttca gtgaagtcaa attagtttct tgttttttct tagccgatta
     4081 taatagctac tttgcataaa ttagtgatgg agtatctatg atcgttaggc aaaacattgt
     4141 tttgattatc gttgcattgt ttactttttc taactcggca gtagctacca atcaaagtgc
     4201 tgaggaaaaa aatcaaacca gcttaataac tgactcagcg cagcaagcag ttcaaccttc
     4261 aaagcactca attctcaaag agaaaaaatc ggagcagcct gaatcaacaa aaccaatcaa
     4321 gcctaatatg gcggattatt gccgaaaaca tacttgctaa ttggtacgga gtattgatta
     4381 catgaaatta atgacgaatt tgagtaatta tattttagct gcctcgctta ccctttcgat
     4441 cgccaatgcc gcagagtggt cttacgagga acagaaacga tggggtagca ttgaagatca
     4501 taaccagcac gaagtacctc atatttatcc ctatgcggtt tgtagcatcg gtcagcatca
     4561 atcgccagta gatctagcga catcgcaggt ttctaagcgg ctgaataact tgaagtttcg
     4621 ttattatgct gacaagcctg atttttttaa tacgggtcac gcagtgcagg tcaatctttc
     4681 aaatgattat caaggagaat tattagtcgg taaagaagcc tatcctttga ttcagcttca
     4741 ttttcacgag cctagtgagc atgtaattgg aaccaaaata tttcctgcag aattacattt
     4801 tgttcatgtt aggggtgatg gaaaaattgc agttcttggg gtgctccttg aaattggaaa
     4861 agataacccg gtgtttcaga ctatcttgga aaatacgcca caccaaggag gagagctcaa
     4921 gcaagatagt gacgtgtggc ttagcataac cagcttgctt cctgaaaaaa gggatgagta
     4981 ttacacttta gctggttctt taacgacacc gccatgcagt gaaggcgtgg attggtatgt
     5041 tttaacggat accaccgaaa tatccgaagc gcaattggat cggctcaaaa gtttcttttc
     5101 tgggaatgct cgactaaagc aagaacttaa caatcgggct attgctaaga agaaataact
     5161 ttccctgtgc ggctacgcta agtctaatga acatgctgct gatgctttga tgtgaccaaa
     5221 gcagtaattt gctctcctgg aataggcttg ttaatccagt aaccttggaa tttcgcacaa
     5281 ccggcatttt tgagtatctc aaattgctgt tgattttcga cgccttctgc tactacatca
     5341 agcccaaggt ttttgcccat attgatgatg gtttttacta aagttaaatc gtcctggtcg
     5401 gtcaggatat ctctgacaaa catccgctca atttttaatt gcgctaattt aaagcgcttt
     5461 aaatagctta gcgaagagta gcctgttcca aaatcatcga tggataagcg aatgccgagt
     5521 gtacgtaatt cattaatttt tttaacggta tcatcaatat gattgatcaa ggaagactcg
     5581 gtgagctcga gaattaggcg gctatgatca atctgtgatt gctcaatttt tcgttttaat
     5641 ttttgcacaa aacctgattc ctgaaattga actgcgctga tatttagtga taatgtcaaa
     5701 gatttaagtg tgtcatgttg ttgccaaaat tgtaattgtc taattccttc atcgataatc
     5761 caatcaccaa tcggaatgat gtaaccatta ttctcgatga gtgaaagaaa ctcattgggg
     5821 ccgacgatgc ctttgtcagg gtgctgccaa cgtaacagtg cttcagcgcc gacaatttgc
     5881 tggctttgat taattaataa ctgataatga agtctgaact gatgcttatc aactgctatc
     5941 caaagttcct tttcgagctg agtccggcgc tctaattctt gctgcatatg aggatcaaaa
     6001 aagcgcagtg tatttcttcc ggcacatttg gcttcataca tcgctttatc agcccacttc
     6061 aaaatatcat ccgctttact atactgatca gaaaacatgc agatcccgat gctggatgaa
     6121 ccacgatgac gaatattatt gactcgatat tcttgatgaa gccgacttaa tattgattcc
     6181 gctatctgag tagtaatgtt catggcgtga ctgtcatcgc tacccagttg ctcaagcatg
     6241 acaacaaact catctccacc caatcttgcg accgtatcag tttcccggat acagcttgta
     6301 atccttttcg cagcttcaat aagcaacgca tcaccggcat cgtgaccatg cgtatcattc
     6361 agaaatttaa aattgtcgag atcaataaat agcagcgcat tataagtttt attacgctgg
     6421 caagcagtga cagtatgtct taatcgatct aagaataagc gtcgattggg taattttgtt
     6481 aacgcatcat aaaatgcaag atcaagaatt tccttttctg aagctttacg ttgactaata
     6541 tcttctttaa tcgcgatgta atagcaaatt tcatcagatt cattggtaac aggcgttata
     6601 gtcatgtgtt catggtacaa ggccccatct tttttcttat tgattatttc acctttccaa
     6661 actttttttg atgaaattgt ctgccacata tcctgataga acaactcact ttgttgtcca
     6721 gaactcatca aatcttttac gttcatacca acggcttcgt gacgacgata tcctgtcaat
     6781 tcggtgaaag caggattaat ccattcaatg gttgcagtag tatctgtgat cattatggca
     6841 tttgctgcag cctctaaagc gatattttga atcttaagct gaagttcaga ttgcttgcgt
     6901 tcggtaagat cgacatgagt tccgattaat cgtttaggat aaataaggtt accgctatta
     6961 tcacgcgcca atgatgctct tgagagaacg gtgacccagt gccccccttt atgcttcatt
     7021 ttaaactcag tttcaaattt atctttgcgc cctgtgacat aatcatcaac taacttgaga
     7081 acccgggatt tatcttctgg gttgactaat ttttcccaag ttttaaggct tggcgatagt
     7141 aattcacgat cggaatagcc cagcatggct ttccaccggc tagagtaata aacataattg
     7201 gtttccatat tccaatccca atatccatca ttagctccgc tgagtgccca ggaaagtctt
     7261 ttattactta agatcaaatt ttctagaaaa atcttgtgtt ttgaaatatc aaccattatc
     7321 cccatcacgt gctgcgcttt gttattttct ggggaataaa ttaattgcat ttcaacgtga
     7381 atgatctgag tattatttac tttaaggcgg cattcggtat gaaaactatg cttgctcgcg
     7441 cttgttcgta ttagttgtag tttttttctt aaataatcat gatcttgttc gtgaacaaaa
     7501 ttaagtaagg agagcttgac agtttttgct acaggaagac cagtagtggt ttcccaagca
     7561 ggattcaggt aaataaaatg gttatgcgta tcgagctgga aaataatatg ggcaccatgc
     7621 ctaatcaatt gtgccgcatc tttctcaggt aattttatct catttgataa ttcaagtttt
     7681 tcgtataact gaatgactcg tttttcgatt ttcttattat tcatatctgt ctatactaaa
     7741 aagtcactga aattaatgtt tatcataaag attctacgca tatctgcaga gataaatagc
     7801 ggtatcaagt tagtcattcc atgcatacta tcgaccaatt tttataagtt tttgaattaa
     7861 gtagaataat ttttgcttaa tcgctttagc tttctaacac aaaaacatca acagataagg
     7921 ttgttttcta aacttgctca aatttttgtt tacttttcgg tgagaacata tccttagatg
     7981 aggatattcg ttaaaattac gctcaagatc gaaataggag ttagagaatg gaaataaaaa
     8041 agtggaccga tgaagaactt gcgtcaaccc ggaataaact tgaagagtgg tgtgaaaaac
     8101 aaaaaaaatc tggaaaagtg atgtggattg ttactgcact tctaggtgct tttgcagtgt
     8161 caaccggagc cgtttttatc ttttttgatg gaattacttt gttgagcgtc gtattaatca
     8221 tcttgggcgt gatgacttgt tttacctggt atcagagcga aaaacgacgt aaaaataata
     8281 tagattttct tgaggaagta aaaagggaaa taacacatcg cgccaaacgt gataaataat
     8341 ggttcgatta ctaacataat caatcaatga caaaggaatg aatttactct cttggataaa
     8401 gcaaattttt tctgatcaag caggaaatca gtttggtatc aggcaaaaag agagtaaatc
     8461 cttggatttc atatttgagc aagatgatat taattgtttt aaacacctat gtcagtttgt
     8521 ttggattcaa ggagaaccga ttccactgat cttcgacttg tctaatcacg tttatgtcaa
     8581 tcagaaaata accttaccga tgatcgaaag attggtggca attgggctgg ttgattatgc
     8641 gccagaaggg tatgtcaaaa agaagcttgg taaacataca cgtttttttt atcatggaag
     8701 attgactaag ataggattcg cgcaagatgc gggaaatcag ctcgatttgg gggtggtatt
     8761 actgaccagc tcaggaaaaa agctctacgc gactttgtca ccaattcgta atcaacagtt
     8821 ctatgagtat gttattcagc attggtttca aaagggttta ttactatctt ccccgcttgg
     8881 ttcaccacag cgtccccact ttgttaatta ggtggcttct cattttctaa taattgccgt
     8941 atggttgcta attctttaag ctgatctttg ctgaggatag gataagctat ctttaaggct
     9001 tgcaaggtct caacaatgat atgggatacc agtaaacgag catttcgctt atcatcagcc
     9061 ggaattatgt accaaggcgc atgatgcgta ctggttgcat ctatgcactc atcgtaagct
     9121 aattgatacg cctcccaatg tttatgggct tcaagatcag ctcgtcctat tttccaattt
     9181 ttgctgggtt catcgatgcg ggcaattaag cgctgccgtt gctcttcttt tgacagatgc
     9241 aagaaaaatt taatgatctg aatcccattg cgatgtaaat agtgctctag gttaataata
     9301 tcttggaagc gttctaccca tattttcttg ggatcagcac attccaaggg caaacgttgc
     9361 tgtttcaaca aatcaggcat tacacggacg actatgactt cctcgtagta agagcgatca
     9421 aaaataccaa ttcgtccccg ttcaggtaag cacttgttgc tacgccataa aaaatcatga
     9481 tcaagctctt ccaagcttgg ttgctggaaa ctaaaaacct ggcagccttg aggattgacc
     9541 ccagacatta catgcttgat aacaccgcct tttccagctg aatccatacc ttgaaaaata
     9601 agtagtattg catagcggtt atacgcataa agcatctcct gtaactgacc aatttcttgc
     9661 tgtgtctcca aaagcaacat ctgatagtgc tctttggagg aataaatagg tttaatgttt
     9721 gtaggtcgct gagctaaatt agcaaaacct acgtttccag tgtgggcaac tttgaattca
     9781 ttgcaatcga aaagcaaatt tttcataaac tgaatcgtta atttgatgtc attttacgtt
     9841 tgatagcgga gtgtgaagaa aacatttcga ccaggttggt taaaaaatgc ggctgtttcg
     9901 taatgttcat tgaataaatt ttcaaggcga gcttgcaatc gccaattttg agcgaatgca
     9961 tactcagcgc gcaaatctac tctgacaaat ccattgagta atcgcgtatt ctcagtatca
    10021 tcgaaacgct taccttctgc cagaaaggtt ccaccaagcg tgtagtgatt gaattgacgg
    10081 tctgcatcaa ttctaaaaat atgtttggcc cggcgcggca aaatcttatc atcgggtgac
    10141 gatgcattct caggattcaa aagtgtaaag tttgcattga ttgtccaatt gagtatttga
    10201 gtattgaaaa cgctttcaag tccttgaatt cgagcacggt caacattgat cgacgaaaac
    10261 gtttggatat caaacgtaat caaattgtta atatgtgttt cataggcatt aactgaccac
    10321 tttccaagtt ttgttgtgct ggtaataccc atttcaaaac tgcgtgatga ttcaggtctt
    10381 aaatttggat ttcctgagaa cgggaaatag agttcattaa aattaggggc tttgaatgcg
    10441 tttccaaaat ttgcggttag acgcaaattt tgagtaattg cataacccca accgattcca
    10501 cccgtgaccc attttccaaa ctgttcatta tgatcccaac gcatcgataa ttgtagatta
    10561 tgtgctgcga tggcagcttg atgctgtgca aataaccccc aattcctacg agaagtttcc
    10621 agataacttt ccgtactttt aacctggtca ttaaaataat caatacctgc agttaatagc
    10681 tgatggttgc caatggaaaa attgttctgc aagaaaaccg agtcacgtat gctgtcaaat
    10741 tggcttaaaa atgtacttct tctgatttgc tcagatcgtt cgattttata agtatctaat
    10801 ttctcgaaac tgcgacctgc agtcaaattt aattgccaaa atgaaaatgg tgaatatcga
    10861 atagacccac cgagtacttg ttgcttcagt ttcgaacgat ttgcataacc accatcgaat
    10921 tgagtatctc cggcactatg cagaaaatgt gcatcaactt cgaatgcttg attaaagcga
    10981 tatcccgcac gtagcgagct tgccaggttc cggtaaccat ctttatcttg atcgtctact
    11041 cgtgaggctt cagtcatgaa gcaacccgct gtttcagaac ctgtgcacgc attaaaacca
    11101 cgcgtaccaa taccactcac attgatgtta aaccaaccct gattaccgcc cccagctaat
    11161 ccagctgatc cattgatcga gttgtaacta ccgccaccaa agctaaaatt aggtttaatt
    11221 tgccccccac ctttacgagt gaagatctgg ataacccccc caattgcttc agaaccatat
    11281 aaactcgaac gcgggccgcg cacgatttcg atgcgatcaa tttgttgaat tggaatattt
    11341 tcaaaagagg tggtacctga agtgactgaa ccaacacgaa ccccatcaat gagaactaag
    11401 acatggtccg attcggttcc acgtaaaaaa aaggaagtct gcttgccagg tccaccatta
    11461 ttaacaatat ttaaaccagg aacacctcgc aataaatctt caaccgatcg cgcttgtagc
    11521 cgtgcaatat cggcttgtgt gattactgtc gcagaagcca aagtctcatc tgcagtttga
    11581 gcggttcgtg tagcagtaac aatgacaggc ggttgaacat tgttagcaga tacgtagcct
    11641 gtagaacata gcgctacgaa aaaaccaaat aaaagacacg atctcatttt ccttccttcg
    11701 cgcacacccg cgcaaaaata ttgactggat gtgatattac aaaggagaaa aaaataaatg
    11761 atgcgctatt ccattaagaa atatgtatca tttgctcgat tgccctccgc aataccttga
    11821 cattcactcg aggccggtct ccgggcttat gagcggaaat attttcccaa tctgcgcctt
    11881 cccgtagtgt ttacagtggc atttgcagat tgttgactca tctaccgttg cgggggcagc
    11941 gctggcctgt accagcttcc cgtttcactc attaagcaaa agcgtaatga gcaccttgag
    12001 cagtgtgcga agttatagta gagggaggga gcgtgtcaag tttattatgc ttttccaaaa
    12061 taagcacaat attaccgacg taaaattgcc caccagcgtg ccatattgag caaagtcatg
    12121 agcgatgcag atacataagt ccaagctgct gcagttaata gcttatgagc atgtaactca
    12181 tcaccttctt taagatattt actctttttc agcagtggta gtgctcgcga gaaactagcg
    12241 ttcaattcca tgggtaaagt aattatatgt actaatgcgg ctgaacccaa actcaaaaaa
    12301 ccacctgcta gcattaatcc accgaccaat ggagaccgaa tgaatataac ggatactggc
    12361 gctagcatca ggatagcagc gccaagtttt tgtgcaggag cgacaagcac tacaagtttg
    12421 gtacgtaact ttagagccaa ataatcatct cggtcctgga ttgcatgtcc aacctcatgg
    12481 gctgcaactg taatagctgt caatgaagca ttattaaatt tatcagaggt caaacggaca
    12541 actttgttta gtggatcata atgatcacct gactcagtta gctcaacttt aacatgcgac
    12601 aaagattctt gatcgagtaa ttgcctagca aattcagccc ctgtatgcgc gtagcggtta
    12661 tcaggatgac tgtatttctc catgacatgt ttgacccagt aagcaggacc aataattaaa
    12721 gcaatgacta aaataatcaa taatagatag agcatgatag attaaaaggc attattttta
    12781 gacttagata ccgcacgtcg cgcgcagttc gattctgtgt tatctgataa agtatcgagg
    12841 gctttatcag aactgacaaa taaactagaa aacggtacta gctaatttag tgagaggcgg
    12901 atggatataa ttttctaaga aaattcttta tttttgtttt taatccaaat cgatcaagga
    12961 aaataccaat ttttgcggaa aatgaatcta ttttcgtgtg tagtaaaaca aggaataaat
    13021 taatattggt ttttctcaga aaaaagatat cagcacaaag tgtgctatta gtagaaaaaa
    13081 actctttatg tccaccagaa ccctttccag gaccttctgt aaagtcaaac atagcaaaat
    13141 gactttcaag aaataaattt tctataacaa cccactgtag tactgtgcca ggtgaccatt
    13201 gtgccatctc tgggatatag ccaaggtact gataaaatag tattccatta gaaattgggc
    13261 aatataaata agcaacaggc ttgtcttggc agaataacaa gtaagcgcga acagagtctt
    13321 tttgagcaag cgcatacatt cgctgaataa ataaatcgtc ttcaggtaaa cccgcagcta
    13381 acatacgctc ttgataagtt ttagtggaaa cttgccttgc tagaggataa aaaatttcca
    13441 tttcagctag tgtcttatat tctcgccaat caatttgatt gccacaatga tccgaaaact
    13501 tttttatttt gcgtttcaat gttgaccgag atttagctga gaatttttct agatattctt
    13561 cttttgaccc tgttagatct ataaagtaac gatgatattg cttaggaaca tatcgtatta
    13621 cgtcttgttt taacgtaaat ctaggtaata gcgttgtaat tggccgtgag cgaaataaaa
    13681 cgccatcaag tttctcgtca atagaatcgg aataatcact ttcctcagta taaggatgct
    13741 cgtattccgt gaagtgattt ctaaatacat tcagcttcag atgtgatgtc caaagggtta
    13801 tttcaccaat tcgaaattta aactctaccg attctacagc gttattactc atagtcttta
    13861 ttatccatac aagcgacgag tcgtttgctc aagatagcga tgagctgtat tgataaaacc
    13921 ggactgtaat acacaactct ctttagattt agcatatatg gtttctggtt gtatatcaga
    13981 gaaccaaatt gttttgaaca aatcgcgatt atttgctaaa aaccggcatg tttgtacaaa
    14041 ccggtcaacc acaattttat caggtcgtgt ctttgcacga tttagcaact ctgatccgtg
    14101 ggataaaatc acaactgaat gccattcgtt aatccatgct tgtctaatta gatttgccat
    14161 ttctttgaaa gaacatgaac caccaaattg aaattgtgac agacgtcctc cccattcttt
    14221 aaagatcgtc aaaggaaatt catatattcc ttctttatat tcctctacat gtaaaggagt
    14281 atctaatcca ttttttatat aaaaacgcgg gttaatgctg ctatcgaaaa taaaaccagc
    14341 ttgaggtaca gcttctagag tttgcaaatt actgccaaaa ttgccagcac ggaaagcaag
    14401 cagattatct gctcctgcct gagctaaaaa ttctttacct tggcagatta ataattgttg
    14461 ctctgcttga ttaaaattaa ataaattata gcctgtttta tttagtggat tatgaaagcg
    14521 ccctctacct agccattccg tatgcaggtg tagttgaact tcttgccccg cttcttggat
    14581 ttgtgcaaca atttcctgta atggcccaat gccataatgt cctgaaaata acgattcaac
    14641 gaaaaaaaca cattttagat tatgttcttt aagctgcgct agttggaatc ctaacccata
    14701 attaccagag gttgtttttc catggataaa tttgttaagg catcgattga actcggtatg
    14761 acccaaatcc caattgtcag gccaaagctc tacatccaca ctaataatta catttagtgg
    14821 tgatttagaa tgatcaatat tttgtttagc aggcatatcg cataaatatt ttctggtgtg
    14881 aactaccgca ttaactaagt attaaatgga ttgggattat tatttgtctt ctggtgctag
    14941 agattaacat ggatgattca tttctacctt atcaatcaat tgtaagtagt ttatatgtga
    15001 cccagggaat tctataacga acttagatag acaaagttta tttctagaca agttttactt
    15061 atttgatttg cattttaaaa acatcgttat atcggtaaaa aatagcatag attgcagatt
    15121 attgcccatt tcatctaatg aaaacatctc tgtttgatct cgctgcaagt tgcttgatag
    15181 caaatgaaat tgaagagaag ttattgctga cccaacaagc aacccaggct tggcgtaata
    15241 aattactctc actggaatct catgaaccga tgcgactaat taatcaacca ggccaaccag
    15301 ctaaacttgt agtagtgaag ccgagtgatg tgccacgacg tcgattggga actaaattgg
    15361 gtctgattgc actgattcac gcaatagcgc atattgaatt caatgcgatt aatcttgcat
    15421 gggatgctat tcatcgattt cgagggttac ccgattcatt ctatgatgat tgggtaaccg
    15481 tggcatctga agaggcctat catttccagt tactacgtaa acgattaaaa gagcttgatc
    15541 atgattatgg cgatttgcca gcgcataatg gtctatggga tatggctttg ttaactgctt
    15601 cggatccatt gatacgtatg gcgcttgtac cgcgtgtgct tgaggcgcga gggctcgatg
    15661 tgacaccggg tataattaag cgcttgcgac aaatcgggga tgaaaagaca attgagatac
    15721 tagaaatcat cctgtgtgat gaaattggac atgtctctat tgggtctcgt tggtataatt
    15781 ttttttgtaa tcaacggcaa atagatccag aaaaaaagtt ttctgaactc gttaatcaat
    15841 actttggtgg gcaaattacg ggccctttcc attatgaggc gcgacagaaa gctggcttta
    15901 ctttgaatga gctgagcatg cttgaaggta tggaaatacc aattagcaga tcaaataatt
    15961 gcaatttttg agcgaatcat tcgagcaact aacaaaaaaa gaatcaagca atttgatcac
    16021 cagccttgaa aattaaacta tcccccatat ttgtaaggat atttaatctc tactaggaat
    16081 tcgtatgcgc cttgataaat ttacaaccaa gtttcagcaa gctctcggcg atgcccaaag
    16141 cattgctttg ggcaaagatc atccctacat agaacctcag catttattac ttgctttgtt
    16201 acagcaagaa gatggcagca cagtttcttt attacaacgc gcgggtatca atgtaaatcc
    16261 tttgcgtgag ggcttagcta aaaaccttga gcgcatgcca aagattgagg gaggtggcgg
    16321 cgaaattaat atttcccgag agctgaataa tttgcttaac ctcacggata aagaagctca
    16381 gaagcatggt gatcaattta ttgcttctga aatgtttttg ctagcgcttc ttgaagataa
    16441 aggtgaaaca gggctattgc tcaaacaaca cggcgcaagc cggagtgcat tagagcaagc
    16501 aattaaatcg gtacgtggcg gtgaacaagt ttccagtgct gaggccgaag gtggccgtga
    16561 agcattaaaa aaatatacat tagatctaac cgaacgtgcg cgtgccggta aattagaccc
    16621 tgtaatcgga agagatgatg aaattcgcag aacgattcaa gttttgcagc gccgcactaa
    16681 aaataaccct gtacttatcg gtgagccagg ggttgggaaa accgcgattg ttgagggttt
    16741 ggcgcaacgc attgtcaatg gggaagtgcc tgatacgctt aaaaacaagc gagttctctc
    16801 cttggatatg gcggcattat tagcgggagc aaaatatcgt ggtgaatttg aagagcgact
    16861 aaaggcagtc ttaaaagagc tatcacaaga cgaaggtcga acaatcatgt tcatcgatga
    16921 gttgcatacc atggttggtg caggaaaggc agaaggtgcg atagatgccg gtaatatgct
    16981 caaaccggct ttggctaggg gggaattgca ttgtgttggc gcaacgaccc tggatgaata
    17041 tcgtaaatat gtcgagaaag acgcggcatt ggagcgacgt ttccaaaaag ttttggtcga
    17101 tgagcccacc gttgaggcaa cgattgctat tttacgtggc ttacaagagc gctacgagtt
    17161 gcatcatggc gttgatataa ccgatccagc tattgttgct gctgctgagt tatctcatcg
    17221 atacatcaca gatcgttttt tgccggataa agcgatcgat cttatcgacg aagccgctgc
    17281 gagaattcgt atggaaaggg attcaaaacc tgaagtgatg gataaacttg atcgtcgtct
    17341 catacaactc aagattgaac gtgaagcgat gaaaaaggaa aaggatgaag cttcacaaaa
    17401 gcgccttgcc ttattagaag atgagatcaa aaaacttgaa cgagagtatg cggatcttga
    17461 agaaatactc aagtcagaaa aaatgcgtgc gcaaggttct caacaaatca aggaggagct
    17521 cgaaaagcta cgtaaagaag ttgaagtggc aacgcgtaaa ggtgattggc aaaaagtatc
    17581 tgagttacag tatggacgga ttccaaagct agaagcgcag cttgctgagc aagtccaaca
    17641 cgctgaagcg accctgcctg aaagggaact caataaaccc aaattatttc gaacacaagt
    17701 cggtgcagaa gagatcgccg aagtcgtgtc acgcgcgacc ggtattccag ttgcaaaaat
    17761 gatgcaaggt gaacgtgaaa aattactgct tatggagcaa aaactccatg aacgtgtagt
    17821 tggacaggat gaggctactc gcctcatatc ggatgcgatt cgtcgttcgc gttcaggatt
    17881 atcagaccct aatcgcccgt atggttcttt cttattttta ggcccaacgg gtgttgggaa
    17941 aaccgaatta tgtaaagcac ttgcttcatt tttatttgat tctgaagatc atctgatacg
    18001 aatcgacatg tctgagttta tggaaaaaca ttccgttgct cgtttgattg gcgcgccgcc
    18061 cggttatgtg ggctacgaag aaggcggcta tctcactgaa atggttcgta gaaaaccata
    18121 ttcggttatt ctacttgatg aagtagaaaa agcgcaccct gacgtattta acgtattgct
    18181 acaagtgtta gatgatggac gcatgactga tggacaaggc agaacggttg attttaagaa
    18241 tacagtcatc gttatgactt caaatctggg ttcgcaaatg atccagcaaa tgagcggaga
    18301 tgattatcaa atcatcaaat tagcagtaat gggtgaagtt aaaacctatt tccgtccaga
    18361 atttatcaat cgtatcgatg aagtcatcgt atttcatgcg ctggatgcaa aacacattca
    18421 atcgattgca aaaattcaat tacgtaactt ggaaaatcgt ctagcaaaaa tggagattca
    18481 tctcgaaata tcagaggcag cattagctaa aattgcagag gctggatttg acccggtttt
    18541 tggggcccgt cctttaaaac gcgccataca aacccaaatt gaaaatccat tagcaaaaga
    18601 actattagaa ggtcatttcg cggcaaaaga tactattcac gttgattgtg aggatgaaac
    18661 aataaccttc accaaatcaa gctaaagaat atagtataaa gctttgcatc aggtaaaatg
    18721 actccttttg agtggataaa gatctatgtt taaaggtagt ctggttgcaa tcgtgacgcc
    18781 aatgcatgat gatggtgcgc tcgatctaga gaaattttgt gagttaatcg atttccatat
    18841 cgcagagaaa tctgatggta ttgtcgtagt tggaaccact ggggaatcgc ccacagttga
    18901 ttttgaagag catcatcttc tcatcaagac agcagtgtcg catgctgctg ggcgcattcc
    18961 tattattgct ggaactggcg caaactcgac gagggaagct atcgaattaa cacaatttgc
    19021 caaaaatgcg ggcgcggcag cttgtttatc ggttgtccct tattataaca aaccgactca
    19081 ggaaggaatg tatcaacact ttaagacaat cgccgaagcc gttgatattc caatgatcct
    19141 ttataatgtt ccgggtagaa ctgttgctga tatttccaat caaacggtag taaagctcgc
    19201 atcggtccct aatattatag gaatcaagga tgcaacagga aatatagggc gtggatgtga
    19261 tctattatct cagataccag aaaacttttc ggtttatagt ggtgacgatg caacctcact
    19321 tgcgctattg cttttaggtg ctcatggtgt gatttcggta acagctaatg ttgcgcctaa
    19381 gttaatgcat gaaatgtgcg ctgccgcttt ttccagtgat ttggcaaatg cgcgagcaat
    19441 taacgataaa cttttaaaac tgcacacgga tttatttgta gaagctaatc caattccagt
    19501 taaatgggca gtaagtcgaa tgggtttgat gggcaatgga atccggttac ccttgacccc
    19561 actttcggat caataccatt cattgcttat tgcagcaatg aaacaggcgg aagtcctgaa
    19621 ttaataataa atttgtaatg caaaatgaag cgattcagta aattatttgg actgttacta
    19681 gcgattatca tgttgatttc aggttgtcat ttattgcctg aaagtcaaaa gatggaatac
    19741 aaatcagcag gaaaattacc accattggaa attccacctg atttaaatgc gccgcaagcg
    19801 gatgatcgct atgcagttcc agatatggcc tcaaccggga gtgctaccta ctcttcctat
    19861 aacagagatc gtgatgaaca accccgatca caagggagtt atgttttacc cgttgcgaaa
    19921 gatactgcac gtattgctcg atcaggaacg caacgttggt tggttgtgca gctggctcca
    19981 gaagatgcat ggccggttgt caaagaattt tggcaagata tgggcttttt gctgaaagtt
    20041 gaagcaccag atgtcggtat tatggaaacc gattgggctg agaatcgagc aaaaattccg
    20101 caagatttga ttcggggctt actttcgact tttctagaca gtatttattc cagtgcagag
    20161 cgagataaat ttcgtacacg atttgaaaga agtgaatcgg gcagtacgga aatttatatt
    20221 agccatcgtg gtatggatga agtttttgtt gatcggagta ccaatcgtac cgcttggcaa
    20281 ccacgctccg ccgatcccaa tttagaggca gaaatgttga cgcgtttgat gatgcgtttt
    20341 ggagtcgaag aagaaagtgc tagaaaagag gtggctgcaa acgaaaatat tatcacgcaa
    20401 gaccaagctt atttagataa aactaggcat ggtgtgttga ttgttagtga aacgtttgat
    20461 cgtgcttggc gtcgcattgg attggcttta gatagagggg gctttacggt cgaagatcgt
    20521 gatcggtcca atggtgttta ttttgttcgg tacgttagtc cagaatatga aagccgtaaa
    20581 acaagcgacg ataaaggctt gctaactaaa cttgcgttct ggcaaaaaag tcaggatgac
    20641 tctagaactg aaaaatttcg catccaggtt gatggcgttg gtgcaagtag tgaagtaaag
    20701 gtacttactt tagaaggcgc tcccgaagaa tctgcagcgg ctgacagaat actaaaatta
    20761 ctctacgaac agctcaaata ataagattta gttttttttc gactaaacac atcttcagta
    20821 cattaaccct aatatcattg ggttatgatg gtatatttct ttatgttacg cttcttaatt
    20881 ttttcgtttg gagctctgtt gccattgtcc acaatttctg tggataagtc tgtgaataat
    20941 tcgtcagtat tcgcgctaag atagttatct gtaataattt tttatgattc gattaagttt
    21001 tatacaatta aatacgtgtt taaaaacagt tggttacaaa aatcctttgt attggtgcgg
    21061 gtttctaagg ctttctttta ttaatcgatc taaaatgtgg attatttcgc aaagtcaaga
    21121 aaaattttta cgattttatt aatcgaaaaa cttgcattta agctacttat tgtcattaga
    21181 tttcccatgc tcgatatcca gcatctgact tatttacaaa atggactccc gttattgagt
    21241 gatgtcaatt ttcaagtatt tgcaaatcag cgtgttgggt tagttggaaa aaatgggagc
    21301 ggaaaatcaa cattatttcg attgatacgt ggagaaattc atcctgagaa aggggaagtt
    21361 aatctgcagg tgggaaaaac acttgcttat gttgaacaag aaattatcaa ttctgctaaa
    21421 ccatcacttg aattcgtatt agatggagat agtgaattgc gtcaactaga agctatcctt
    21481 aagcaagata atcatgacgc taaatggttt gatgcacaac aacggtatga attgattgat
    21541 ggttacggtg cgagagcacg tgcagctcag ttgttggatg ggttgggatt ttctaatact
    21601 gaacatgatg cgcctgttag caagttttca gggggatggc gtatgcgctt aaatttagca
    21661 cgtgccttga tgcatcgtgc agatttgctt ttgctagacg aacctactaa tcacctcgat
    21721 ctggaagcaa ttttttggct agaacactat ttgcagaatt accccggcag tttaatcatt
    21781 atttcgcatg accgagaatt tcttaatgtt tgcgtaaatc gcattgccca tattgagcat
    21841 cagaaaatcc aaagttacag cggaaattat gatgattttg aacgcgctcg tatggagcgt
    21901 attgctcaac acgaacaaac ttatgagcaa cagcagaaac aaattgcaca tatggaagat
    21961 tttgtacgtc gttttcgagc caaagcaacc aaagctaagc aagcacaaag tagaattaaa
    22021 gcgctagaaa gattaacacg ggttgcgccc atccaagttg aaaatggata tttctcctta
    22081 caaatccttg cgccggataa tggccctgat acgttgttga aaattgatag catgggattt
    22141 ggatataccg atgaaagcat gttatttcaa aaggtgaatt tatgcttaag aacgggtgat
    22201 cgaattgtgt tattagggcc caatggcgtg ggaaagtcca cacttattaa actgcttaca
    22261 gcagaattat tacctgctaa aggaaaagta gagataaatt cttcaattcg cattggttat
    22321 ttttcacaac accagcttga gtatctcaat ggtgctcaaa cgccattaca gcatctacaa
    22381 caattagccc acaatgaaac gacactcacg ctgcgtaact ttttaggtcg ctttgggttg
    22441 gcagatgaca gcaaagatag acctatcgcc actttttctg gtggagagaa aagccgtttg
    22501 gcgctagcct tgctcgcttg gcagaaacca cacttgttac ttcttgatga acctaccaat
    22561 catctcgatc tcgatatgcg agatgcgctg atcgtcgcat tagaaaatta tacgggcagc
    22621 cttgttatcg tatcgcatga tcgtagctta atacgcgctg tagctgatga gctatgggtt
    22681 gttgctgata gcaatgtgca tctatttgat ggtgatttgg atgattacaa agattggctc
    22741 gaaaaacgac gtgataatgg aacagataaa gctaaaccaa aaaaacaaag ccaaaccaag
    22801 cgtgctaccg ctaataaaag gtcaatgctt acacagcaaa ccaaaataga agctgcacta
    22861 gcaggagttc agcaagagct tgctgcactc aatctccagc tttctgatcc tgcaatctat
    22921 gctaatgctt cgagagaggc catcagtgaa ttgaatctca agcgttctga gcttgaaaaa
    22981 aaaatcgatg cgctggaagc aaattggtta gaactagaaa gccttatgga agaatttggt
    23041 aaagtatgat actcaccgat tgaagatttt tgcaaaatat tgcgcattaa ctcatgctta
    23101 tcgtgagcaa gttttattta ttgtttgctt gaagaggaaa atagtctatg aaaacgaaat
    23161 tctttatatt aaagctgatt actatatcgc ttatccttaa ttttcctata aatgcggcaa
    23221 atgcgatgcc aggtatgcat gggtcggagg gtaaaagcca tacagaagct ccgcgcgtat
    23281 cgaagagtat gggtgataaa attaatacca caggtgccga actcgagatc acctacagtg
    23341 ctgatggcaa gacagcaatt tttgtttcaa cgcgtgaagg cagtatcgag tcacctggat
    23401 caccttataa ttttgatatc tggatttcta aaaatgttga tggggagtgg caaacaccta
    23461 ttcatttagg acctgatatt gatcccacac taggacctaa catcaataca tcagcctggg
    23521 aacttgaacc aagcttctct gatgacggaa atgtcattta tttcacgcga tatgagccgg
    23581 gaaatttatt atcaggggat ctttatgtag ttcagaagat cgatggtgtt tggcaaacgg
    23641 ctaaaaattg gaatgacgtg ccagagttac ccaatattaa taccccaaca ggtgaggaac
    23701 attgtccaat tattgcttcc gatagtctga tttattttag ctatcatcaa ccaggtgtta
    23761 cgcaagatag tgatatttgg aaagtagaga agaaggatgg ggtatggcaa caaccagaaa
    23821 gtttaggtgc taaaatcaat tcacctcatc gtgaccatat gcattggact ggattatcaa
    23881 aggatggtaa aagtttgatt atcaccagta tgcggcctga tttgggttcg agaggagggc
    23941 acgatgaatg gatatcgcat caagatgaaa agggagaatg gcaagtgcca gtcaatcttg
    24001 gtgatgcagt caatacggca ggggaagata tgtgctggac ttttacgcca gatggtaaaa
    24061 agtttacggg tagctggggt ccgtatagta cttatgatat ggatattcgc tggatcaaca
    24121 aagatgatgt tcctttattg aaaaattttg agccaatcgg ccctccaccg aatttactga
    24181 ttaaccggaa atcaacccag aatacaagtc agtagcacta atttaatctt ggttgggttg
    24241 atttctgtaa tcaagttgaa tttatataga aaatccacgc tgtcttaacc tggcttcttc
    24301 gtcatcgatc aattggacgc attcgtttgc tccaattgca tcgatacagc tttcttcaat
    24361 atgatattta agcattaata atccacgaag ctcctggcgt cttggattat caccttgaag
    24421 acaaactgag taatttcctt taatgagtga aacaatagta tttttctgag cgatatcaac
    24481 cccacaagca atcgcaagcc taccgatccg ctcatcaagc tcaaaaagtt ctttgtcaaa
    24541 atgatttctt cccatttgaa atacttagac agcccccagg tttttcattt acactgaaga
    24601 tagcgaatta caaatcatca atatcagtaa atttttaatg atcatgacta tgtccgccac
    24661 ctttatcatc atgagcgcta tgattcatct tgatcgtgct gatgagttga tttttttgct
    24721 cggcggataa aacgttgtgt atggctcgac gttcttgaat atgatgttca accattgcgt
    24781 taaatgcatt tgcatgatct tttcctaagc ttgaaagctg ctcgtctgtt gaactaggat
    24841 ccatcttagc tttcttgaaa gcgtgcatac tatcttttaa tttttccttt attttcttat
    24901 gcgcttcttc atgttttagg tggagtcgaa caagttttcc aagctgctcg ttagttagtt
    24961 ttaacttatc tgcatggaaa atgatcatat gcgcgtagtc atgtttttct tccatcatgc
    25021 cgtgttggtg cttatttttc ttgtcctgcc catgttgatg gcttgagtta ggctgattat
    25081 tctctttttc ttgagcgtta cttttatgat cgtgctgatg agcttcagcg actagaatag
    25141 attgagcatg caaattgtca tttgctttta aaggggtact caaacttatt aatgcaaacg
    25201 aaaaaaaaga actaactacc gcgaaaataa ccgatttgtt atttggcatt ttaaggtatc
    25261 tccttaagtt ggatcgaatc ataaagttct agaatataaa tggctctaga ctaacatatc
    25321 ttttctgcta gcctagagcc attgtaccgc tcaagctgat gtatccaaaa gcacaaattc
    25381 cctgtattca attattgctg ctcaggaatc tggatccgga aaagcatatc gcgcttggaa
    25441 aagctttagc atcgctacgt gagaaaaata ttctgattat cggatcaggt gtcatttcat
    25501 catttgaaaa cattttttac ccacgatatc gacagcagta gagaaaatga tgcatttgat
    25561 caatggttaa ttcaaatgta cgcctgtccg gaaattttat ctgaagtacg cgaacaacga
    25621 ttgatcgaat gggaaaaagc accatttgct cggttttgtc atccgaggga agagcattta
    25681 ttgcccttac aggtttgtta tggtattgct tgtgctgact cccccatagc caaggttatg
    25741 tttaatgagc agattatggg aaagaaagtg acgagcttgt tgtggtagta gaatcagaat
    25801 cacatatggg aaagtactaa ggcttaacac ctgtaacaat actatgataa ccaaatgctt
    25861 tataaatctg aatgtcttca aatccaactt catcgagcat ctttgttaat tcctggccag
    25921 aatacatttt tccttctgtc caacccatca tgatcatact gaacgcggca atagcaaatg
    25981 gcccagtttt gtcgtcgttg taaagcattt catgaatcac aattcttcca cctgactcaa
    26041 ggttgccgaa gctcttggct gtcaaaaaat ggcatttatc tggcatccag tcatgataca
    26101 tgtttgagta aaaatgaaga tcagccattg gccagtgatc ttgccatatg ttagctgcgt
    26161 gaaccttgac acgattttgc attttatact gagcaatata tccttcggct acttcgcata
    26221 cttggggaaa atccattatt agtgcttgct ccagattggg cagttttatt gctgcgccta
    26281 tggaatgtgc gccagagccg ccgccaatat caagcatgat gcggtgtctg gaaaaatcca
    26341 tgatggttgg ccaaaccaat gctgaagtga tgctaatact gtgcatgccc gctgtaaaac
    26401 ggcgcagtaa ttcgatctgc tcttcatgtg ttttaaaaag attattacca ccatatactt
    26461 ggggtttatc ggtgaggata gcttcttcta agcttgcaat cgaagatgcc tgatagttat
    26521 ctatcatcat atcccagaaa aaaccgaagt aattcgggct gttctcgagt agatatactt
    26581 cggtaaccgg cgttaacctg taacgctcct catggagaga tagaaatccc agtgcagttg
    26641 ctgtagttaa tattgcctca acagggcgcg gcttaaggtt aagcgcgtta caaatttccg
    26701 ataatgtgca tgtattctta gccagggtag aaaaaatttt gagtcgatgt gccagaagta
    26761 aagcaggata accataaatc gcaaaaatta catcccataa cggacggtcg tccacccccg
    26821 gtagttgaat attggtgatt ttttcttgtg ccatgattat tccttttcgc tgatattatt
    26881 cctattattc gtgcttcagt ttttcagttt caacgagtcg tttgatattc tgaaccgttc
    26941 tggtaacacc atctttgata gaagcacgaa ccagtccctc aaaggggcgc ataatcacaa
    27001 gtaactcaag taactcaaat gtaaatatta gttttgtcga gttatcgaga tcgccagctt
    27061 gcaattcata cgaacagcga tagggatcgg atacgccaga gaatgttaag cgtttttctg
    27121 gggtgaaatc agatatttta aaacgagatt ctgagcggcg tccttggtca acccgtactt
    27181 gccgtccaat tgttccaaac tgtactgggc cagcagtaat tttttcaagt tctttaactt
    27241 caggagacca tttgggataa ttctgaaata gattttctcc tatatattta aacagctcac
    27301 tggcagagca ttccacaatc gtagatgcgc tgcctatcac aggctccgta gaacctagat
    27361 tcaacatgga acctaagtta aacatattta tgtcaccccc tttttgccag aataatggcc
    27421 acagtctggc gatggaaaac ttcatgcatg ctttctctct gcatcatatt tatgatgtga
    27481 atcatttata ttttatcgtt aatgctcgtc gtgatctttt ttatttcttg caggatgaaa
    27541 gtgcacatca catttaatta gcgtttataa agttaattga agtaagctac agtagaactt
    27601 ttgctactta aactaaatga tcacaaaagg agccgggtga aagctaccag caactcaccg
    27661 tacagaaata atagcagatt tacaggttac gaatactgag ttgacagata ctaaaggacg
    27721 gacccattgc atagctaggc acttcaattc ctgatcaagc aaagtacttt gattcagaat
    27781 ttcttataag atattcttca gggtgtcggt aagttatata ccaagccact atcaaaagtt
    27841 gcgcttttat gcatttccaa tctgaattca caatactaaa aaaggaagtt atcaatgtcg
    27901 ctgaaagaaa aacttagtca gtttggcccg ggattgctct atgcgggggc tgctgttggt
    27961 gtttcgcatt tggtgcagag tacgcgagcg ggggctgagt ttggcttcca gttagtagcc
    28021 attgtgattt tagtgaatat ccttaaatac ccaatctttg agttaggtcc tcgctacgcc
    28081 gctgcaacag gtaaaacatt gcttgatggc tatcaccaga ttggtgcatg ggccgtgtgg
    28141 atttatttct tactcagtgc gttgacgatg tttattgtga tgggggcgat ttctattgtg
    28201 acagcaggat tatttgccca gctaacagga atgcctctgg atgccccaat tattgctatg
    28261 ttgttattag ttttgtcggt acaaatacta gggtttggtc attattcgct cctggatcgc
    28321 agcatgaagt tcatcatgat cgtcctaacg atctctacat tacttgcact tattttttct
    28381 ttcttcgcgc ccattgaaaa gcaagctgag tttgaaacga catttaattt cttggaatca
    28441 gcgcatatac tttttctcgt tgcattaatt gggtggatgc ctgcacccat tgatatttcg
    28501 atttggcatt cggtgtggtc tgtttctaaa aacaaggagc agggtcatcg cattccattg
    28561 gctcaagcct tgcttgactt caaggttggt tattggggaa caatggtact ggcagtgtgt
    28621 tttcttactc tcggcgcact cgtcatgtat ggaactccgg aatcaccggc tagtaatagc
    28681 acagagtttg ctaaacagtt tattggtttt tatacgacta gcctcgggga atgggcgttt
    28741 ccgatcattg ctttagcagc atttgcgacc atgtttagta cgttgttgac ttgtctggat
    28801 gcttatccac gcacattacg tcgatcgact gaattgctgt ttccccgact cgatagtttg
    28861 ccctatcaca ataaaatata ctggtattgg ttgatattaa ccgcactggg tacgattgta
    28921 ttactccatt tttttctacg agatatgcga gcgatggttg atttagcgac aaccgtttca
    28981 tttgtattgg cgcccgtttt ggctatcttg aattacatgg ccttgaaacg atctgaggtg
    29041 cctgctcatg ctaagctgac gggtgcatcg cagtatgctt accttgcatc aacgggtatg
    29101 ctaattgcgt ttagcttgtg gtttcttgcc tataagccct aaatggggat agatggtaca
    29161 tcttgattag gtttggggga gtatattgcc cgagttaaat cgcgcttccc atgaatttat
    29221 ttttctaaac caattaattt ctgacttagg gtatttgtag tccatcgctt tattgatgtg
    29281 atcgccgaga tcacagctgg cgtttgcgat aaaaatccct ggttgacctt gccgcaagga
    29341 catttcaccg aaatgagttg aaagaatcgg aattcctaag gagcggtatt cataatactt
    29401 aatgggatcg attgctgctg ttaagtcatt acgcttgaat ggaatgagcc caactgtaaa
    29461 cgataacatc gcttgaattg cagcgctgtg ttcgcaagca ggtaaaaatt tgatattttg
    29521 agggagcgag gctgggattt tggtaaagat gggaccaatt agatggattg ttgaggtact
    29581 gaccgtttcg gcgagtttag ctacaaacgc ccaatcaaac caatccccaa tcgttcctag
    29641 gtaccccagt acttgttttt ctgtcgcacg agtttttagc gtatcgttga tagagggaag
    29701 ggcatcaacg gcacaagcgt tatgaacaag ggttagcttg gattgatatt caataaaacg
    29761 ttcagtgagc ttagtcgaag aagctgatat gcgtgttact tgttttgcta tccgttgctc
    29821 acgattcatc attgcaaatc tcgatatgcc gtgataaaac gcaggaaaat catccatggc
    29881 atcgtatatc gacttgaggg aagggtaacg gaatagtact tgtaacgcca gttctgaggg
    29941 cttgccaata cctaataacg tttcgttctg agattcaatg aaacgatcga ttgcttggag
    30001 aatatcattc catagccagc gatacacata acaagagccc ggtagtggct ctataggcgg
    30061 aacaaatggt tgaattaccg ttaaccacgg tggcttatta ccattgccca cgattacatt
    30121 ttttgataaa aattttgatg attttattct tcggaaatcc cctagattgg gtaaccttgt
    30181 ggggtaaggg tctatccaaa gtacctcacc gccatgcttg ttgtgaaacc attcaacaaa
    30241 ctgatgcggt ctttggctaa agctatccca tggtaagggt gaaagataaa ccagttgcat
    30301 taaggaagcc gctcctctag cacttttaca atgcgctccg ctgcatggcc gtctccatag
    30361 ggtgagattc cttttctcat ggcgaaataa gctaattcat catccaacag acgctgtgtc
    30421 tcgcttagta ttctatcgta atcatgacct acaagttttg caacaccttg agttactgct
    30481 tcagggcgct ccgtttcatt gcgaagaacg agaacgggtt tgctgagtgc aggtgcttct
    30541 tcttgtattc ctcctgagtc ggttaggatc aaaaaagaac gtttcatcac tgcaacaaaa
    30601 tgtgcgtaat caaggggagc acataaatga atatttttct ggctcgcgag caattcattt
    30661 acggtttcag ttacgttagg gttagggtgc actggataga gaaattcaac accagggttg
    30721 ttctgggcca aagatttaat agcttggcag atagatcgga gtggctcacc aaaattttca
    30781 cgacgatggg cagtaatgag aacgagtcgt ttatccggat caaggttaat aggtaatgcg
    30841 agattctgat cagcaagcga taataaggcg tcaataacgg tatttccggt aataaaaata
    30901 ttattatccg agataccatc acgcattaga tttaggcgag actctgcagt cggcgcaaag
    30961 tgccagcgcg ctaatttccc agtaataaca cggtttgctt cctcagggaa aggattatcc
    31021 atgtcccagg ttctcaatcc tgcttcaacg tggccaatcg ggatatgctg ataaaagcaa
    31081 gctaaggcaa cagccattac ggtagtagta tctccctgaa ctaaaactgc atctggtttt
    31141 tctgatgcta atacttcttc tgcactgagc aaaagccgtg cagtcaatgg tgcaagtgac
    31201 tgattgggcc gcattatatt caaatcgatg ttaggcacaa tgccaaataa gtcgagtact
    31261 tgatcgagta agtttcgatg ttgggctgta gcgagaacgc gaacttgaaa tgattttttc
    31321 cttaactcaa gaatgacagg cgccattttg attgcttcgg gacgggtgcc aattacacaa
    31381 agtatggttc tattaatgcc tatactcatg tggattccat ttgccccatt tagactcata
    31441 ataggctttg tttttctcaa acaaggcttg cttgtctcga tcaggaatct gattaaatga
    31501 tgctgaaaga tgatgatgaa tgaaaacatc ttcggcgcaa gcaatttgta ggtctagtcg
    31561 atcaattcgt ctgcaataat catcatcttc aaaaaagcca cgaccataat tctcgtcaag
    31621 taagcctact tgttgaaaaa catttctaga catcattaca caaaagaagg cagcagttct
    31681 cagcttaaaa tttttcccca tatgaggaat agtatgcgat aagatgcgag ggagcatctc
    31741 ttcacttaag agcgggtaag taatgtcaat ttttgcttcg ttcccaatat tatttgttac
    31801 agctccaatt attccaatcg aaggatcagc ctgtaaatgg cgtagcatcg ttaatatcca
    31861 tccaggcgtt accacggtat cgttattgag cataacaaga taatcacctg ttgcggcaat
    31921 aaggccaata ttattgcctt tggcaaagcc ttcatttgtt ttatttcgaa taacttggat
    31981 tgtgggatgg cgcgtattaa gctcagttag ataatcaggt gtgttatcag aagaattatt
    32041 atcaacaatg ataatttcga gattaggata atcgctccat tgcagtaagc tattcagaca
    32101 gagttttgtt agattaagat tattgtaggt caaaacaata acacttacct ttggcatggg
    32161 tatttgttga atagcttgca ctaagtcgtt tacacgatga ttccaagttt gcttacatgc
    32221 aaactgcttg cgtaattctg tttgattttc ttcagggcta gatgatttca aacaagtgga
    32281 aaccaatgat ataaactcat ctgaggtttt agctcggtgc acgagatttt caaattggga
    32341 aatctctgga agatcgacgc ttacaacagg ttttccactg gccagatatt cataaatctt
    32401 cacaggattt gtcgcaagtg ttagggggct aatcttaaat ggcagtaagc aaacatcaaa
    32461 agcatataag tagaatggga gtcgattata ggatacctca cctataaaaa tgatattggg
    32521 tatcgccttc gaaatatgag atgcattaat ggtatcgtta ccaataagaa gtattagcgc
    32581 attaggctcg gccaaggcca ggttttttac taattcgaga tcgaaccact cagcaatcgc
    32641 accataatag cctattattt tacgaccttt gggatccaag tagatttcaa tgggtggatt
    32701 attaaaatgc tcgtactcac tagcattgcg gatgattaca acattattat tatattgctg
    32761 ggcctgcttt tctagccatg cggaagaaac gatgatttga tcaaatttac acatcatctc
    32821 tttttcgagc ttaattagat cttctgatac atttccaaat ccttcatggt gatccataca
    32881 atcgtaaatt cgaagtgagt tgggtaggcc tattgctaaa ggataccagt atgcatgctg
    32941 aataattgaa acacttgata gcgttgcaaa atcaataaaa aaattcgtga tgctcagctg
    33001 caaattattt agatgcttaa ccgtaggtga ggaaaagtag attgctggcg catcctctac
    33061 atagagtttg atctggtaaa gctccaagtc gctatccaat cgttcgatcg catacccagg
    33121 tgttggtgaa tcaatgaaat gattgctgaa gtagaacaca cgtttcccga gtttagctaa
    33181 acttcgcgca agatgttgag gtcgttgcag ccggaagtgc caatcaatta ctgagaaaat
    33241 taagtaatca gatcgactgt ttaatttttc tccattgatt aaggcacgta aattatctgg
    33301 aacgagtggt ttatggtcta atgcacttcg agatatttga tttacagata ctggaaatgt
    33361 ttttattatt ttttttaaat agggtatttg agacaggaaa aacttaaatt tttgtttata
    33421 acgatgggaa atcggaagtg cgtgatagaa gttttttgcc gatttgagca ctaatttttt
    33481 agttccatag tgaaatgggc ggttatctat tttttgtgcc caatccgata actgtatcgt
    33541 tttttcatat tccagtaacc gttttatttc caattgatta atttgatcat cacgttgagt
    33601 tagcgtctgc tctaagtttg aaatgtgtgt atcgcgttca gcgataacct gataaaactg
    33661 ttgttgcttg agattatatt ctgataaggt ttgctgataa tgggtaatat gctgattttg
    33721 ttcaaggatt gtttgcttga gctggacaat ttgttcagtt tggtgcgtga tttcatcgtt
    33781 taggcgttta atttgattat tgagattttc aatgtgtgaa tctgcgtcaa aagattcgat
    33841 acgagagtca tttagcgata acaataattg atcaatccaa tctgaatggg catagagtaa
    33901 gatgatctgg tatccctgcc aagcagttct tggcctggtt ttgcaatcaa taacacgaga
    33961 tacaacaagc ctaaaattat tcttgagcat caacgggata ttttcaggca ggaatgaaaa
    34021 gaaatgttca tcaatccgat ctatttcttt ataggggagc gcaagtacga ccgcttttct
    34081 tgcccgcttt aatatcgagg tgagtacctc atagggtttg tggaaatgtt ctaaggtatt
    34141 cgacgagaaa atgatatcgt agactatttc attgcttatt aagcttttgc ttagccaatc
    34201 ttcattgagg aaacagattg cagggtagcg ccgtgttgct ttctcaatag cagctaatga
    34261 gaaatcgatt ccagttattt gggaggagtc aatgtaactt gcccaaacat ctgaaccgtc
    34321 gccttgagcg catccccagt cggcgaaggt taaattgttt ctcttgattt gctcgataaa
    34381 ccatttaggc aaatgttcta tagcgatttg agaaaaaaag cgagattgcg ctggcccctc
    34441 aaagctatcc caattacttt tgaatcgatt attccagtat gcgttggagt tgatagaatc
    34501 cataagcata ttatttcagg ctaactgctt gttcaatact aggctggaaa agtccaaaaa
    34561 atcgctcttt cgacagcgat gcaaagtaat gagcaccttc gaaatattca taataatgaa
    34621 tatcacggta ctgccgattt tctactgctg ctaccagtag gtattttccg gtcactaagg
    34681 gattcgttaa tttaaatgaa atactgaatc gttcatcttc aggtaagcgt tttaattcaa
    34741 aatcatgagt cgtcgaaacg attaaatcgg tcccttttaa atctttaatc gaaaatgcaa
    34801 tgcttaagtg atcccgagaa atttcgggtg gaatacgcag atcgataaaa atttcaatag
    34861 gtgcacccca gtggagcgta ttttttctga cgccatgact atcccgtacc gatgcagaaa
    34921 ggataatacc tttataagat ccccagtggg tgataggttt tgaatcgagc gcaatctcgg
    34981 gtcgtgaatc tggcggtgat gtctgtccat tcccggttat ttcaccttgc aaggaagaat
    35041 cagcctgatt ttcgataggc agttctgata gttttgtaga tttagtttta tggcgagcga
    35101 tatttttatg cgctaaattg gatgcttcga tggaaggttg atcattatta aacatatagg
    35161 ccatatagcg ccctgttact ggaaaaacat cgccatcttc tacgagatat ccttgatcaa
    35221 gccacagggt gcggctgcat agcgtccgaa ctgaagatat gtcgtggctc acaaaaagta
    35281 cggttgttcc tttactcata atatctttaa tacggcgcat acattttgct acaaaacgtg
    35341 cgtcaccgac tgcgagcgct tcgtcaacaa tgagaatttc tggatcaaca tgaatcgcga
    35401 ttgagaaagc taatcgtgcc tgcattccgc tggagtaggt tttaatgggt tgatcaaaaa
    35461 agtctccgag ctcagagaat gcacggattt tttccatttg ttctgcgatt gcatccctag
    35521 agaaacctat aatggcggca ttgagcagta cgttctcacg gccggtaaat tcagtattga
    35581 agcctgcgcc gagttcaaga atggctgata cacgcccaat cacattgact gtgccgatcg
    35641 ttggagagag tgttcctgca atgatttgca gtaaagttga ttttccactg ccattggttc
    35701 caattactcc gatgatctct cctttagaga ttgtgaagga tatatcacgt aatgcccaga
    35761 attctctaaa gaattttttt ttactcgcgc caaacatttg taacaatcta tctctaggtt
    35821 tatcgtaaat gtgaaaacat ttacctaacc catcagtttg taaagcaatt tcagaggaca
    35881 tcggcaaaac ctttacgcgt tttctggaac caggcgaagc caagccaagc ggtaacgatt
    35941 gagaataatg agtaaataat ccaagcactc caattaagtt ctttacccca aatcattatt
    36001 tcacgtgcct gttcaacgat aaaggttagc gggctaactt gaatgaagta atgatattcc
    36061 tcgggtaaag cagaaattgg atagaaaatg ggtgagagaa acagcaatgc tgttgttaaa
    36121 attccaatga tttgagtgac atcccgtaaa taaacaccga gcgatgctag gagccaggtt
    36181 aatcctaaaa tcatcagaaa gaatgggaac aacaagactg gtaacaatcc tatcgtgctg
    36241 gggggaattc caataaaaat caaataaaaa cataaccaaa cgaacaggct gataataaga
    36301 tgaaacgttg ctgagcctat cgcgacaata ggtaatattt ctaagggaaa tactactttt
    36361 ttaacgtaat taacattgcc aagcacaaga ccgggggctc tgttgataca ttctgcaaat
    36421 aggttgtaca tcattagccc agcaaaaaga actaaagcaa attcggtctt ggagtcagtg
    36481 cccccgatcc agcgtgcttt aaatacgata ctgaaaacaa atgtgtaaac gattagcatg
    36541 agtacgggat taaagaatga ccacaatatt cccataatcg agcctcgata gcgtccaatg
    36601 atctcacgtt ttattagatt aaaaataaga ctcccgtgag cccttagtga tttcagtaaa
    36661 taaaaggggg aagtgggcgg cgctacgtgt ggattcaatt tttagaaagt taaatgtatg
    36721 cgaatactta ataggctaaa atgcactatt gttattacgg gttgagttaa tgacaaacac
    36781 gatttttttt gagttcaaac tggtcaaggc atttatgtaa tggtagcatg cccgcttcta
    36841 tcccagcaat tcgaatggag ttgctgctaa aatggaatga gttgcaagac caccaaattt
    36901 taaaataaag cacataacct atttttttca ggatgaaaat tggtacctgg aacgttaatt
    36961 cattaaaagt tcgtctacct caggttattc aatggctaga ggttaatcaa cctgatgttt
    37021 tatgtttgca agaaaccaaa ttacaagata gcttctttcc gctgacagaa ttatctgcca
    37081 gtgggtattc atcaatttat gtcggccaga aaacctataa tggggtggca ttgcttagta
    37141 agcaaccagg ttgtgatgtc attacaacga ttccaaattt tgaggatgat caaaagcgag
    37201 ttgttgcagc aacttatggt gatattcggg tcgtttgtat ttatgttccc aatggtgaaa
    37261 gtatcgattc agagaaattt caatataagt taaattggtt atcagcgctt aatcgctggt
    37321 tgcacgagga attaacacaa tttccaaaat tggttttact cggagatttc aatattgcac
    37381 ctgcagatag cgatgtacat aatcctgagc tatggaaaga tcaagtatta tgcagctctc
    37441 ctgaaaggga tgcttttcag aatcttttaa atctcggttt atacgatagt tttcgattgt
    37501 ttgaacaacc tgggaaatct tatacgtggt gggattatcg gatgcttgcg tttagactaa
    37561 ataaagggtt aagaatcgat catatcttgt taagtaaagc gttaactgaa agttgcgaat
    37621 cctgcgtcat tgataaaaca cccaggaaat tagaaagacc ttctgatcac acgccggtag
    37681 ttgtgacgct aaaaattggt taaattcaaa tgtggttttt ctgaacgatt actagaagtg
    37741 cgcagccact agtcaggcta ataatcagca ccaatcttat agagttctag ctcaagccga
    37801 tcgatatcat catcatataa ttccatatca tcaataataa gctgtaattg ttcttcaggg
    37861 ctgagttcaa cctcatcagt ctgatttgca cttatctcag tgggctgcat taccttttct
    37921 gatttagctg atgggataac tctggattgg tgcgtgccaa tttgttctgt ttcattatgt
    37981 tgcaatgact catctgcttc catttgggct atcgagggcg ataccttaga tagcctttgg
    38041 gaagtaggta aaacagtcag cactggcaat tctgcttgct ctgccccctt caggtgatga
    38101 tcggagtaaa tcgtttcgcc cctatcaccg aagtgtttat agatgcttgg ctcagcaaag
    38161 caaatactgt gagcaaataa gattgcaaat aacaagactg ttttcatcat actcgggtac
    38221 aaatatcaaa ggctatttag ctctttgcgc aaggcgataa tgtttctttc atgcactgaa
    38281 gctaaattca aaagtgtatt gatattatca ttttcaaaat cattgatggc agcagacaca
    38341 ggttgcgaac tttccgattg aatttctttc atttttattg aaatcaacct tagcgagtta
    38401 atgatgccta ccagtaactc tgtttcgata gccagttcat tttccaaaat ctgacggcga
    38461 tcaaagtcac gtttcttttg ttgatgattg tctatcttag gataaatccg accacttgat
    38521 agcgacgcat gcgttgcttt tgcgtaattt ttcccttggg cattggcgta agttacacgt
    38581 cctttattgt ccactgtttt atggatattg gatgatgcac tcacttgggc cgtgatggta
    38641 aatatcgcta agagaattag cgtaattgag ataatttgtt tcatgaatac ctccataatt
    38701 gaaatagcta acagcgagaa aaatatcaag tggcgtatct tgatatttcc tctattttca
    38761 atcaaaaagg actcaagcaa acaaacaata aacgctttaa atcaaccctt tatctataag
    38821 ctgtagtaca tcgaaaattc tatggggtgg ggtgctgttc gatagagaat gacttcctcc
    38881 atttttagag cgatatacgc atcgatcata tcattagaaa acacgcctcc ccgcgtaagg
    38941 aattcacgat ctttatcaag atgttctaac gcttcttcca aagcattgca ggtcgctgga
    39001 actttggatg cttcttctgg cggtaggtca tatagattct tatccatggg atcgcccggg
    39061 tgaatcttat tctgaatacc atctagtcca gccatcagta atgctgaaaa tgcaagataa
    39121 gggttggcga gtggatcagg aaaacgaatt tctatacggc gttgtttggg gttgctggca
    39181 tggggaattc ttattgctgc agagcggttg cttgcggaat aagcgatatt aacaggcgct
    39241 tcatagccag aaacgagtcg tttataggaa tttgttcccg gattggtaat cgcatttaat
    39301 gccttggtat gcttaatgat cccaccaata tagaataggg ccatttcaga catgcccgca
    39361 tagctatttc ctgaaaataa atttttacc
//
LOCUS       DAOQKQ010000002        32720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_524_length_32720_cov_6.224583, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000002 DAOQKQ010000000
VERSION     DAOQKQ010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 32720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32720
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_524_length_32720_cov_6.224583"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /gene="thiL"
                     /locus_tag="PKZ49_00200"
     CDS             complement(<1..872)
                     /gene="thiL"
                     /locus_tag="PKZ49_00200"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HND35007.1"
                     /translation="MESEFDIIRRYFTRPTKKALLGIGDDAALIAYNATTDLAISTDT
                     LVVGHHFFPDTDPSQLGHKALAVNLSDMAAMGAVPRWLTLAITLPRDAPQTNSAWLQA
                     FAHGFFALADRYQVELIGGNTTNGPLNICVQIIGEIPRNKALKRSNAQLNDDIWVSGQ
                     LGNAALALAHLKQQLVLTSEALDVCLPALHTPTARVALGQQLIDLAHSAIDISDGLLA
                     DLGHILSSSNLAAVIQLETIPHSWVWNRYAAHPQALEWLLSGGDDYELCFTAPETERK
                     KIHTLSKELATPLT"
     gene            complement(973..1467)
                     /gene="nusB"
                     /locus_tag="PKZ49_00205"
     CDS             complement(973..1467)
                     /gene="nusB"
                     /locus_tag="PKZ49_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HND35008.1"
                     /translation="MKEAPAKSGPKPAPKFKNRRRSARELALQGIYQWRLTASEAGFI
                     EAQLRSASFFARVDAVFFSDLLQGVIKHADELEQKIAPCLDRTFQELSPIELSILLLS
                     TYELIYHPNIPYRPIINESIELAKTFGGTDGHKYINGVMDKLATQLRGTEVALHAKKT
                     AKFH"
     gene            complement(1479..1958)
                     /gene="ribH"
                     /locus_tag="PKZ49_00210"
     CDS             complement(1479..1958)
                     /gene="ribH"
                     /locus_tag="PKZ49_00210"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HND35009.1"
                     /translation="MGYYDNIPEIETNLDGSELRIGIVMSRFNIEIGEGLLGACTAEL
                     VQLGVQEANIQLVTVPGALEAPVTLQKMALSDQFDALIALGAVIRGETYHFEVVANQS
                     AGGLMKAQIETGIPIINAILTTDTEDQASSRMSQKGKDAARAAIEMANLFDKLVEFE"
     gene            complement(1999..3111)
                     /gene="ribBA"
                     /locus_tag="PKZ49_00215"
     CDS             complement(1999..3111)
                     /gene="ribBA"
                     /locus_tag="PKZ49_00215"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011113009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HND35010.1"
                     /translation="MTISSIETIVSDFKAGKMVILVDEEDRENEGDLVLAAEFVTPEA
                     INFMAKFGRGLICLTLTEERCHQLKLSPMVAANHSSHGTNFTVSIEAASGVTTGISAA
                     DRARTIQVAVKKEAKPEDIVQPGHIFPLKAQKGGVLVRAGHTEAGCDLAQMAGLMPAA
                     VICEILKDNGEMARLPDLVEFANTHQLKIGTIADLIHHRSLTESLVSRIAERTIQTVF
                     GEFDLKAYHDKTANITHLALVKGQLQSEKEILVRVHEPLSVIDVLDIADCSHSWSIND
                     AMRVIAEAGSGVIVMLHRKGDAGILTERIMATKSWYPDQIKTDLRDHGIGAQILKDLG
                     VRKMRLLATPRKMPSMTGFGLEVTGYLESSNKQTAT"
     gene            complement(3120..3722)
                     /locus_tag="PKZ49_00220"
     CDS             complement(3120..3722)
                     /locus_tag="PKZ49_00220"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HND35011.1"
                     /translation="MFTGIIEAVGIIDQISQNPKGVSLQIDAGKLDLSDVKIGDSIAV
                     NGICLTVTKQGECYFAVDVSHETLRCTKGLKNLGTQVNLEKAMRLSDRLGGHLVSGHV
                     DGVGEVIQFEPEGENYRLSIQAPDALLKYIVVKGSVTINGVSLTVNRIEGKVFDINLI
                     PHTLAETNLKLLKVGEAVNLETDLLARYVERLMTQSINQI"
     gene            complement(4239..6050)
                     /locus_tag="PKZ49_00225"
     CDS             complement(4239..6050)
                     /locus_tag="PKZ49_00225"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HND35012.1"
                     /translation="MEASQPDTKNPIWIARETLKHLATQRIAPTPDQYQAIYYQIAGI
                     NPPKHDVSDSLANNLLAILRTLSLPQAANRYVVDINLAVGNENWPTVAQLVGQLIQLQ
                     ASPNQQLERPWEELLLMLWDARGKASDDHTRRDELAYTVALFAGQPEKLNHELNTLIT
                     HWLNKPHEIEVFDNENSPVEIMPMDSLAQENCSQTQYPWQQWQALVVNTLRDGLIPRL
                     TSHQHWIDEIEFIIQAIEAIEQPENIDWIQKRLRQCWIHMALRIDQEQRLIDGLLSVL
                     ALFVEHLSLPSDLDPWVTHQLDNIRRNLAKPLTLHRIYDAESILKELVYQQGLLQHTL
                     TEAQQTLKTMISVFLERLGVVTEATGEFQHKIDHYTERVHQTSQAVDFKSLLEELMED
                     TRHMQLDMLRARDELVKTQQQADAAQQRVNELRQQLRQISEHNRDDRLTGALSHRALV
                     ETFSAEQSHMSQSGKPLCLALLDVDNLSELNAQLGRQAGDDTLTGLVQVIKTVIREGD
                     SVIRYGSEEFLILLPNTPLSTATDLLRRLQRELTKRFFMNNNKRLLITFSAGVTRCLS
                     QESREQALDRADYAVFLARKQGKNQVAVVEAPEIPVN"
     gene            6375..7973
                     /locus_tag="PKZ49_00230"
     CDS             6375..7973
                     /locus_tag="PKZ49_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35013.1"
                     /translation="MTRFSKLNIRLTAITAAVMSAFAINVQAATATYNPSDNTVDLPI
                     VEVLDGATSNFFSAKLQLVNGNELVLVDAQPIAPAKNVQRNVYDSATTAVHIASVDAG
                     GAEFYAKLKLIPGSNPLRFSLEQLVNNQFESCPDFATPGPIANSCILSGEIRNRNITL
                     TKNTQWILSGGVFIGGDKTENATITINPGTKIVGQQGADYLWIRRGSKILAEGTPDNP
                     IVFSGPNEQAAGEWGGVVLNGFARINGCNEGTAVCEVANEAITTEFSGGNNDADSSGI
                     MKYVQILFAGFAVRPDEELNGLTLNAVGSGTVLQFIQVHRGLDDGIELFGGTANLKHI
                     VLSGIEDDSLDWGLGWRGKAQFVLIQQSASDGDHGIEADNNPNNPDIAPRSKPVLSNM
                     TLIGSPVSQASGALLRRGTGVNIWNSIFTGFQNCLTLDGTATSALVGQASELSINNSF
                     VNGCQNSFAESGGATASTADWFNSQTGNKAQDPLLNGFLPNAGSPVLQAGATLSDSYF
                     KPVSYAGAFRDANDNWTQEWTFPF"
     gene            8319..9167
                     /locus_tag="PKZ49_00235"
     CDS             8319..9167
                     /locus_tag="PKZ49_00235"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HND35014.1"
                     /translation="MSESIMATAEMLQDSFFRDSTSSIPEVGQCATTKLESQTNIVTT
                     WARHLSEVREAQKLRYQVFATEMGASLPNILSGYDIDIFDDYCEHLLVRDRKTWQVIG
                     TYRVLTPAQAKLAGKLYSDSEFDLRALQPLREHLAEIGRSCVHKDYRQGNVILALWKA
                     LVQFMTQNKLDDIVGCVSIPMNMQVKDGQIGMGYAAASIWRQMKESHLASQELQVIPR
                     LPLPVEQLDQTLDIDPPALVKGYLRVGAKVLGAPAWDPDFNTADLPMLMRMRNLPERY
                     RKHFLG"
     gene            9170..10111
                     /locus_tag="PKZ49_00240"
     CDS             9170..10111
                     /locus_tag="PKZ49_00240"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HND35015.1"
                     /translation="MRDHYAYLHNQFNKPTDNWQSNSELDDSPEENYKSSVRAIFISD
                     LHLGTTGCQASALLDFLKTYSSEQLYLVGDIIDGWQLRRRWYWPQAHNDVIQKLLRKV
                     RKGCKVIYVPGNHDEFAREFLGHSFGGIEIVEEAVHETVDGRKFWVIHGDYFDGVIQC
                     AKWLAYVGDNLYEFTLKLNRYLTSLRARLGLPYWSLSQYLKYKVKTAVNYVLSFEQAV
                     AAEASRRGLNGVICGHIHHAEIRDINGVTYCNDGDWVESLTALVEHFDGSIELIHWPQ
                     HALQLTNTVEIPLTEGCLPIGKPVVTNKIKDNEKVLA"
     gene            10108..11118
                     /locus_tag="PKZ49_00245"
     CDS             10108..11118
                     /locus_tag="PKZ49_00245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HND35016.1"
                     /translation="MKIALITDAWQPQVNGVVTTLVELVAELEKFDHQFMIIHPGLFN
                     TRPCPGYAGIDLAIRPRKHLQKLLLQYNPDAIHIVTEGPLGWAARSICLKEGWFFTSA
                     FHTKFPEIFKASLGVPVFVGYTVLKHFHQPSSGVMVPTQSVLEGLQKRGFQNLKSWSH
                     GVDTELFRFHEKPAEFPALGRLPRPISLFVGRISYEKNIQAFLDLDIPGSKVVSGVGP
                     LESDLKRRYPNVLWLGILPRNELVQLYASADVFVFPSAADTFGLVLLESMATGTPVAC
                     FPVDGPLEVIGDSGAGAMLPDLKEAVLSAFEIPREKARERAETFSWGSAAKRFADYLV
                     PV"
     gene            11183..12220
                     /locus_tag="PKZ49_00250"
     CDS             11183..12220
                     /locus_tag="PKZ49_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35017.1"
                     /translation="MYRRLGYRFLLRRYLPQWVRISILQQPISTQYEMHSALVYEPPG
                     DSIHTIIAFPGLSVHGYRDHRMDAVARAFSYLGFRVIIPCVSDIEQLIIHPSTISKFA
                     ALIEAIATHSELNPNHRQLGIFSASYSGGVALLAASHPQISSSIKAMCLIGTFADFRN
                     VIRFVLDQEEIDEYARFILLRNLLSQSSYRNPEVTQLFDIAVADNGFKKKNPLLPYSL
                     QNTSKEATHFFCQLLEDAFFRHQVAQSALEEIDRREHWLNRFDLDKQLTHIDFSISLI
                     HGYHDQVIPSHESIFLHKRLAQLGKKSHLILTSLLDHGDVVLNKNFFVETDKLAQGLG
                     FFVHELCESTS"
     gene            complement(12295..13611)
                     /locus_tag="PKZ49_00255"
     CDS             complement(12295..13611)
                     /locus_tag="PKZ49_00255"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0008889 - glycerophosphodiester
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HND35018.1"
                     /translation="MDFERTMAIIASCMLFSITFSMVTKNSLAASDHSQKDIDQVKVS
                     ETKRTNIQLGPRPFFLVDDMSEGALKNKLKSCANKVSRKTEFSIGHRGAALQFPEHTK
                     ESYLAAARMGAGIIECDVTFTQDRELVCRHSQCDLHTTTNILAVPELAAKCSVPFSPA
                     EIDPITGEIIKPASAQCCTSDITLAEFKMLNGKMDASNPAATTPEAYLGGTPAWRTDL
                     YASKGTLLTHRESIELFKQLGVKMTPELKAPSVPMPFQGDFTQQDYAQKLIDEYKLLG
                     IKPEKVFPQSFSLSDVLYWIQNESEFGAQAVYLDGRYDDPTFDPQNSASWNPSMSDLV
                     SQGVKIIAPPMWVLVTVENNRIVPSVYAKAAKEAGLDIITWTIERSPPLIANGDWYYQ
                     TIDSVVNKDGDQYVLLDVLTKQVGILGIFSDWPATVTYYANCMDIK"
     gene            complement(13988..14503)
                     /locus_tag="PKZ49_00260"
     CDS             complement(13988..14503)
                     /locus_tag="PKZ49_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35019.1"
                     /translation="MKKLSLILVITTIMLVSSFNLMAACAPWVQTRVTQPDYAVLDAA
                     LTSAIQTRLKNKLNATKDEPTYSALRIEAEGLAATIANGRVVITMPDGTVVVDTSKGA
                     NNTYDNFKAKTINENHNSRIAILHSQLYDCGLGLETKLSTTDNTKENYLARRLGAYLN
                     SAGTVRLSQKL"
     gene            15149..17266
                     /locus_tag="PKZ49_00265"
     CDS             15149..17266
                     /locus_tag="PKZ49_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoX family phosphatase"
                     /protein_id="HND35020.1"
                     /translation="MSSKLHPDSKLKINDITRNESNNPSMMEIIEQRKISRREFLKSS
                     VTATAGITTMTLLGTAAMESMASFAEDISKHGLLLGFNPVSPNLQPMIDGITVPEGYT
                     ARVLISWGDSINDKPDWDTVSAMDEETQLHCYGAHTDGMHFFPWPGQAGNQRGLLVSN
                     SEYCDPPLVNNITPASDYAKVPITLAMVRTQQAAHGVNIIMIEKKRKKWSLSRNWYFN
                     QRITGNTPCVISGPAAGHELMKTAADATGKNVLGTLNNCSHGYTPWGTYLTCEENWNG
                     YFSNESGDVIGELDLDRKLNILKGQSRYGVVKGGFGYRWHEVDERFRADLHPHEPHRF
                     GWVVEIDPRSPWDKPIKRTALGRMKHEGAQVVVDRQKHVAFYMGDDERNEYIYKFVCA
                     KKWNGNPQVTDPDMLDKGTLYVARFNADGGGDWMPLIWGQNGLTPENGFSSQAEVLIK
                     TRQAADRLGATMMDRPEWGAAHPKTGEVYMTLTNNSRRGNTPASSNQIDGTSNAGSAN
                     PSVDAANPRPDNDYGHIIRWREDDANVTATHFSWDIFVQCGDRDSAKALGASYNPAGS
                     DGYKGNINGDDYGAPDGLWFDKDGRLWIQTDQQGDAKGDWINIGGNVMMCANPQTGET
                     RRFLTSPPNCEVTGVVTTPDGRSMFVGIQHPGEDWKINFTDNSSWPDNGYNGPTTFTG
                     AVVSKPRSSVIVITKDDGGVIGT"
     gene            17516..18349
                     /locus_tag="PKZ49_00270"
     CDS             17516..18349
                     /locus_tag="PKZ49_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HND35021.1"
                     /translation="MTADNNNPRLAVLIDADNTFKVIDLIDELFEEISKFGDASVRRI
                     YGDWTQNQLSRWKEILPKHAIQPIQQYANTKGKNATDSALIIDAMDLLYTAPLEGFCI
                     VSSDSDFTRLAIRFRESGKLVYGFGEEKTPESLQAACNEFKYFEILSQNKHIGIGDSV
                     ATQAEDIPNKSKTSLKKKTTKSVSFSSEVTTIPGTKKNSRELAMDTKLVSLIRSAIEA
                     ISDEDGFAYMGEVKKHIVKNFSAFDSRNYGYAKFSDLIKAIGLFEVDTQNQHIKDKRK
                     K"
     gene            18984..19754
                     /locus_tag="PKZ49_00275"
     CDS             18984..19754
                     /locus_tag="PKZ49_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3450 domain-containing protein"
                     /protein_id="HND35022.1"
                     /translation="MKKCFEGLLTLIGLSIITLYAPTVTADNLENLAKSLAKIRGEVE
                     ELQSQLDLEKENHNSRIASLSSQLADLSVEERRQKISIEKLQQSIEKLTAESKQAEHS
                     GDNLKPSLILFLAEYKRFIQAGLPFKIEDRLKAIQELEDNLNNKLIDPKKAVNHAWSL
                     LEDEVRLSKENGIYQQTIELNGEKILADIAKLGTIFLYFQTRDGRFGMAQHTEGSSWK
                     FVSMDTKKDKTQISQLFDALKKQIRQGYFELPNPLKKL"
     gene            19751..21286
                     /locus_tag="PKZ49_00280"
     CDS             19751..21286
                     /locus_tag="PKZ49_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HND35023.1"
                     /translation="MKPFLLVGLFCYLISTAWAQETTPEVSQATETITTEPASDKKTN
                     TNNPGKAASAKKNTTSDSKPVNLETAYKREYAFLDAQKRELKAQLDKYKASANSKEQE
                     LIRKINALERESVSRSAKIDQFNSQIVEAERTQAAVSERNDALEITYAQSELTLKQYG
                     IEMPPPIKEGKENDLEKTTFLLQQGLALIQKLGEIQSKAGKFFLESGKQAEGSIIQLG
                     NIAAFGVSADGGGALVPAGNGEFKVWKEPAAEIATALNNNEQPDYLKLFLFESRTTAI
                     DETPDKTVLSIIESGGLIGWVIVGLGALALLLAFIRAFLLRKNSTDNKQLADRIIALI
                     AEGRLAEAKKICESGGSAISRVLASTLRHLKDDREHMEDIVHEAILQEAGPLDRFGSA
                     IMVIASVAPLLGLLGTVTGMITTFDVITEFGTGDPKLLSGGISIALVTTELGLIVAIP
                     TLMLGSLLNAWARNIRRDIEHSALRATNAFLGGKSGNSVATDPGAGNDPETQSFRLQP
                     QPI"
     gene            21308..21901
                     /locus_tag="PKZ49_00285"
     CDS             21308..21901
                     /locus_tag="PKZ49_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HND35024.1"
                     /translation="MTLSEIVSLVEELFQAGGLVMPPLFCCVLLLWYGLGYRFWVLKT
                     PQKMGVRDLLSFYREGKFRDAQSIVEQAIQKGVALKKKQSQYLSYQLDAAFYEFEREI
                     ARFSVLVKIIVIISPLLGLLGTVIGMVETFDSLASMALFTQTGGIAGGISQALFTTQM
                     GLAVAIPGLLAHSFLSRKQQQIEKDLLQVKKLLCRLT"
     gene            21932..22336
                     /locus_tag="PKZ49_00290"
     CDS             21932..22336
                     /locus_tag="PKZ49_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HND35025.1"
                     /translation="MRYSEPPETETAIDMAPLIDIVFILLIFFMVTTTFVKDMQLELE
                     RPKASSAVSSSSKAIRLFIDRNGDTYMDGEPVRIWLIQSKLRDLLSTSTNKVVLVVTD
                     EGVPAGKLVEVVDQARLAGAQSVGVATRKEAG"
     gene            22361..22999
                     /locus_tag="PKZ49_00295"
     CDS             22361..22999
                     /locus_tag="PKZ49_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HND35026.1"
                     /translation="MIKKPMNKHIAATISMLMGPILVFGMVILMNKFADSLDKTPAQE
                     VTEISMVKQPPPEPKQEIKKVEPKKQPVRSNTPPPFKGLGSSLSGIDLGLLGFDNGGM
                     TGVDESLLGKTGNAVMTEDLVDVPPKPKSRSSFRYPPAAKKNGIKGYVVLSVLVDTDG
                     SVKQVQVLESNPSGVFDDAALQGVRSWQFEPAKYKGDSVRVWAKQKIRFDLS"
     gene            23021..24187
                     /locus_tag="PKZ49_00300"
     CDS             23021..24187
                     /locus_tag="PKZ49_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HND35027.1"
                     /translation="MMLIATKRAIIVAIVLISGYFQPALAKEEKPQSDDFIELAAIML
                     KDGHYDRALLALQSVNLDDEKTDLARFYTLQGLAYMNLNDFDASKDSFQTAIKHGQKD
                     PLIYAYLAQIHFSLKDYQGVLNVIDKTGEHKSKNPALTSIQAQSYWHLQKTVQAIETL
                     NKGHQLFPSDYRFLKLKVFYFVQLQLYQEAANLGREYLARSKAQAEEYVAIGNALRLS
                     REFQEALKIMEIARLKFPDNEVVAKLLAHIYLDQGMLNAAAHILEQAARINSDLLSEA
                     AEVYRRAGRLHKALMLNSGINDQKVKLKQRLSILLALKQYDQAASMEASLYRVGLFDD
                     QSIRYALAYAQFSSGRFQQASKHLDFLTEPELFKKGIELRRLMEVCKEEPWKCA"
     gene            24512..27688
                     /locus_tag="PKZ49_00305"
     CDS             24512..27688
                     /locus_tag="PKZ49_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HND35028.1"
                     /translation="MALVITTNETFASTESAQFSINLFQDGLPISGAEVSVISDQFGR
                     PDARFIDSSATSYAWLAKDGSPITTSANGSIAGKLPPGIYQIRIRTQRDQFFSFELPL
                     RPTEDVQILVTFYDDPEKTLLNIESSEAGVLAGTEKAPITAESSGEGTIVAQVVSAET
                     QKPIKDVQLFISGLNQRLRTDDQGQVQINIPIGAYSVSLLHSAYNSQTRDSVEINKDQ
                     LTELKFSLTPAGVELAEYVVLEPHLAGSLTAVIEEQKSSAEVATIMSAEQFSRSGDGD
                     AASALRRASGLTLVGGQFIFIRGLGERFSSTLVNGAAIPSPDPTRRVVPLDIFPTSVL
                     ESVLVQKTYSPDRPAEFAGGTIELRTRGIPDQFFFNFNGSIGVIDNSTFAKGLTYKGG
                     DLSFLGYDDGTRALPNSLADIVKDGGKLEPKTPFNPNGQTPAELEQIGEGLATDWDIK
                     RKDNPPLGNMQAAMGDVFNLGNFRAGYIASAGWNQEIRNQNEVSKLFVATQRNDDQLL
                     KVQDFDVKRTLREVQLNGYFGADVEYKDKHRLFTKTMFLRQSVDEGRISEGFTDNNPT
                     EIRRTRLRLFSNQLLMQQVGGSHTLDWAKDLKIDWLYSHSTANREEPKFREYRYDQLL
                     SNDQIFAFSRQPDSNQTMYLDLKDKDQSWRVDAKLPVEFSPNYRFSLESGMITQSRTR
                     HSGIQRYAFFPFGPDARNQTILNQPSLEDILNPRYIGPNGFQVRDITRPTDSFRANQD
                     LLSYYGKVDANLYEKLRISGGLRWEDNLMLVETFQSVGNQNRPIQSKLDRVDMLPSVS
                     ATWSVSERQQLRAGYSQTISRPDFRELSPAPFTDQNTNRETIGNPNLKQTSITNYDLR
                     WEYYLSPNENVLAGFFWKDLKLPVEVVNVPGNAGLLTFQNADDARVYGFEVEGLKNLE
                     FIHTQLRNFTVGANYTWSDSLVNLNEKSLEVQTNASRPLQGHSRHIINFQLGYDNREW
                     GTQATLLYNVASQRVESVGVLGAPDRFEQPFHMLDFVVGQNVNKWLSLRLNMRNLLDD
                     DFLVKQGDEVVRQFRRGREFSLGIRINF"
     assembly_gap    28244..28343
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <28344..29500
                     /locus_tag="PKZ49_00310"
     CDS             <28344..29500
                     /locus_tag="PKZ49_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35029.1"
                     /translation="GLMTTNSVNAEGDGGGFDGGGFDGGGFDGGDFDGGGFDGGDFDG
                     GDFDGGDFDGGDFDGGDFDGGDFDSGDFDGANESPETDGSTLGESIEDNSPADASSTS
                     ADAVEQPAQESPEAGDSSATNDQSQESSETVDQGSDEAKTESTGQTADTADNTATDQS
                     TDNSASNTQTEPGTQAGDANQSDQSASGGDSSQTDQTNQDNANSTDNSNQNVNIQNNI
                     NTDQNSGHHHNDWGLNHGHHHHHDGFGLGLGVGLGLGLGFGPFSPFAPFGGFGYYGFG
                     YPFGGFGYYNSFGGVGFYNSFVGFSPFNRFGYPYGGFSAFAPAVPLVSSPMVMTPARK
                     PTYIQQRSYTRSAPAPKTNYWHYCRNPEGYYPYVKKCPGGWLKVLPQPAS"
     gene            29526..30086
                     /locus_tag="PKZ49_00315"
     CDS             29526..30086
                     /locus_tag="PKZ49_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HND35030.1"
                     /translation="MLKFAKSVMLCSTALLVACVHIPSGPSVLVLPGSGKTFEQFRND
                     DYLCKQYAAEQVSGRTPRQAAITSGMESAALGAALGAAAGVAIGGGEGAAIGAGTGLL
                     AGALGGSSSAQTSGYLGQQRYDMGYLQCMYANGHRIPSPGRFATEYTDGGSNRYPPPP
                     PPRTGSGKFQPPPPPPGNPPPPPPGY"
     gene            30128..30625
                     /locus_tag="PKZ49_00320"
     CDS             30128..30625
                     /locus_tag="PKZ49_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35031.1"
                     /translation="MKSLLLVCSAILGLSLMSFSLADPMSVFKGRAIFIAADNQLTVL
                     PDSPVQALSCRFENGIFVAGSSPSIAIPIPGAIDVKIIGTHAYVATSSTENSSATTGY
                     AKYDVTACLPDGPFTPYRARANLTSGELVIPCVEVSGSEYNVVMKQRGKSSNWEVDFV
                     GTGCN"
     gene            complement(30733..31656)
                     /locus_tag="PKZ49_00325"
     CDS             complement(30733..31656)
                     /locus_tag="PKZ49_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HND35032.1"
                     /translation="MDRFENMSMFISVVEAGGISAAATRMNIAKSVISRRLKELEAHL
                     GVELFHRTTRRMNLTDSGRAFYQQSVRILADLIEAEHATSQFHGALKGSLKVALPLSF
                     GLMHLGPAINVFLEIHSEIRFDLDFNDRQVDLLAEGFDLAIRIANLPDSSLIARRIAP
                     INVILCASPAYLDRNGRPDSPAALAHHQCLVYNLTSNFEYWSLRDAEDQVTKVKITPY
                     LKASNGEFLRDAAVNGLGIALIPTFIAYQEIEKGALVPILDKYHYSSLAVYAIYPQTR
                     HLSQRVRAFIDFLIKRFEGSPYWDSYLKNHE"
     gene            31785..32654
                     /locus_tag="PKZ49_00330"
     CDS             31785..32654
                     /locus_tag="PKZ49_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HND35033.1"
                     /translation="MNKLSEASRIIPATSIREGAGVIVNRTIGTPMLRHYDPFLLLDH
                     ISSDQPQDYLAGFPAHPHRGFSTFTYMIDGHMTHHDSMGNHGELDPGSAQWMKAASGV
                     IHSEMPKQQLGLMRGFQLWINLPAAHKMDRPEYREYSEKMFPVLRFPEYTVKVLVGHF
                     EGVNAPIIDPVTEVTYLDVQLQPDKAFRYDLPNDNSSFIYVFEGYGQLASQAVQRHSL
                     VILDASDNNPITFLAGNKGARFIVVSGKPIHEPIVRYGPFVMSTKEQIDQAMKDFQSN
                     QFVRERAWVNQNH"
BASE COUNT         9244 a         6517 c         7357 g         9502 t
ORIGIN      
        1 cacgtcagtg gggtagctaa ttcttttgag agcgtatgaa tttttttgcg ctcggtttcc
       61 ggggccgtaa aacaaagctc gtaatcatca ccgcctgaca atagccattc gagggcttgc
      121 ggatgcgctg catagcgatt ccaaacccaa gaatgtggaa tggtctcaag ctggatgaca
      181 gcggctagat tcgaacttga aaggatatgc cctaaatcgg caagtaaacc atcagaaata
      241 tcgattgcgc tatgcgctag atcgattagc tgctgtccta gcgctaccct tgcagttggg
      301 gtatggagtg cgggtaaaca aacatcgagc gcctcactag ttaaaacaag ctgctgcttg
      361 agatgggcaa gtgcaagtgc ggcgttaccg agctgccctg agacccaaat atcatcgttc
      421 agttgtgcat tgctacgttt taatgcctta tttctaggaa tttcaccgat aatttgaaca
      481 caaatattaa gtggtccatt cgtcgtgttt ccaccaatca actcaacttg atagcggtca
      541 gctaacgcga agaacccgtg agcaaaagcc tgtaaccagg ccgaattagt ttgtggtgca
      601 tctctgggta atgtgattgc taaagtaagc cagcgtggaa cagcgcccat tgccgccatg
      661 tcagaaagat taactgccaa agctttgtgg cctaattgac taggatctgt atctggaaaa
      721 aaatgatggc ctactaccaa ggtatcggta gatatagcaa gatcggtggt tgcattataa
      781 gcaatcagtg cggcatcatc gccaatgccc agcaaagctt tttttgtggg acgtgtaaaa
      841 tagcgacgaa tgatatcaaa ttcagattcc acgctctaaa ttatggtgtt gactgttttg
      901 ctaatcgtaa tgcgattcta gaatcgtgca ggtcattttt gttgagcaag tgaatagatt
      961 tttatggtgg actcaatgga acttggctgt tttctttgcg tgcaatgcaa cctctgtgcc
     1021 acgtagctga gttgccaatt tgtccatgac accattgata tatttatggc catcggttcc
     1081 cccaaaagtt tttgcaagct cgattgactc attgataatt ggacgatagg gaatattggg
     1141 gtgataaata agttcatacg tactcaacaa gagtatcgac aactcaatgg ggctaagttc
     1201 ctggaacgtt ctgtccaggc agggtgcaat tttctgctca agttcatcag cgtgtttgat
     1261 gacaccttgc aataaatcag aaaaaaaaac cgcatcaact cttgcgaaga agcttgcact
     1321 tctcagctgt gcttcgataa aaccagcttc gcttgcggta aggcgccatt gatagatacc
     1381 ttgcagcgca agctctcgtg cagagcggcg tcgatttttg aatttgggag caggttttgg
     1441 gcctgatttg gccggtgctt ctttcatggc aactgttttc attcaaattc aactaattta
     1501 tcgaaaagat tagccatttc aatggctgct ctcgctgcgt cttttccttt ttgagacatt
     1561 cgtgacgaag cctgatcttc ggtatccgtg gtgagaatcg cattgatgat cggaatgccc
     1621 gtttcaatct gagccttcat taacccgcct gcagattgat tagcgacaac ttcaaaatga
     1681 taggtttcgc cccgaataac ggcacccaat gcaatgagtg catcaaattg atcggacaat
     1741 gccatttttt gtagcgtaac gggagcttct aatgcaccgg gtacggttac taactgaata
     1801 ttggcttctt gcacaccaag ttgtaccagc tcagcagtac aggcacctaa taatccttca
     1861 ccaatttcaa tattaaagcg actcatcacg atgccaattc tcaattcgga gccatctaga
     1921 ttagtttcaa tttctggtat gttgtcgtaa taacccatgc taattcaatt cctatgtgcc
     1981 ttttttaaac acgtatcctc aggtagcggt ttgcttgttg ctcgattcta aataaccagt
     2041 cacttctaaa ccaaatccgg tcatgcttgg cattttgcgc ggcgtagcca gtaaacgcat
     2101 tttacgaaca ccgagatctt tcagaatttg agcgccaatc ccatgatcgc gtaaatcagt
     2161 cttgatctga tcaggatacc aagattttgt tgccataatg cgctcggtta agattccagc
     2221 gtcgcctttg cgatgtaaca taacgataac gcctgaaccg gcctcagcaa ttacccgcat
     2281 cgcatcgtta atactccaag aatggctaca gtcagcaatg tccaacacat caatgactga
     2341 caacggttcg tgcactctca ccagaatttc tttttcagac tgaagctgtc ctttgactaa
     2401 cgcaagatgt gtaatattcg cagttttgtc gtgataggct ttcaaatcaa attcaccaaa
     2461 aacggtttgt atggttcgct ccgcaatacg cgataccaaa ctttcggtta aactgcggtg
     2521 gtgaataagg tcggctattg taccaatttt aagttggtgc gtgttcgcaa attccactaa
     2581 atccggcagt cgggccattt caccgttatc tttgagtatt tcacaaatta cggctgctgg
     2641 cataagtcct gccatttgag cgagatcaca acccgcttca gtatggccag ctctaaccag
     2701 aacccctcct ttctgcgctt taagcggaaa aatgtgaccg ggctgaacaa tatcctccgg
     2761 tttagcttct tttttaacag cgacttggat agttcgtgcc cgatcagcag cagaaatacc
     2821 ggttgtaaca ccgctggctg cttcgattga aacagtaaaa ttagtcccgt gagatgaatg
     2881 attggctgct accatcggtg ataatttaag ttggtgacaa cgctcttcag tcagcgtcaa
     2941 acaaattagt cctctaccaa actttgccat aaaattaatc gcttctggtg taacaaactc
     3001 ggcagccagg actagatcgc cttcattttc tcgatcttct tcatcgacca gaatgaccat
     3061 tttgcccgcc ttaaaatcgc ttactattgt ttcgatgcta ctaatcgtca tagtcgtacc
     3121 taaatttgat tgatcgattg agtcattaaa cgctcgacgt agcgcgctaa taaatcggtt
     3181 tccaaattta ctgcctcacc tactttcaat aatttcaaat tggtttccgc taatgtatgg
     3241 ggaattaaat taatatcaaa tacctttcct tcgatacgat taaccgtaag actcacgccg
     3301 ttgatggtta cagatccttt aaccacgata tattttagca acgcatcagg cgcttgaatg
     3361 ctgagtcgat aattttcccc ttcaggttcg aattgtataa cttcaccaac gccgtccaca
     3421 tgtccactaa ctagatgtcc gcctaaacga tccgatagcc gcattgcttt ttcgagatta
     3481 acctgagtac ctagattctt caaacctttg gtgcagcgta atgtttcatg agaaacatcc
     3541 acagcaaagt aacattcacc ctgtttcgtg accgtcaagc aaataccatt aaccgcgatg
     3601 ctatcaccga tcttgacatc gctcagatca agctttccag catcaatctg taagctaact
     3661 ccttttgggt tttgtgagat ttgatcaatt attccgactg cttcgataat tccggtaaac
     3721 atctgtacac tccttaactt gggggaggtc actaataaaa cttactgtgc atgagattag
     3781 aaataagtat ttttttatcg aggcgctgag tttttctcaa gattttaagg taatggcagt
     3841 atatttttgc tattttcaac agttaaaatt tatctcttag taccagaaaa tattttctct
     3901 cgactactac ctcataatat caggtagcaa aaaaacaact aacataatat tactagcctg
     3961 tgacggtctt gctgctatac ttttctaaaa ttaggacatt gaaagcaaat cgaacagtgt
     4021 agcgccattc cacgctatat atcaggagaa ggaaaagtaa atcatgcgaa taatattagt
     4081 tattagctgt atggcagttt taacggcttg tgctacttcg acaccgactc tgcagctagt
     4141 gtgaatttgt gattcttctg gtctagaaga gatgggggca gattatctag aggcagagaa
     4201 atggttaaaa attgcggcta gctagaggtg catcgggcct aatttacagg tatttcggga
     4261 gcttcaacta cagcaacctg gtttttacct tgttttctcg caagaaaaac agcatagtca
     4321 gcgcgatcta aggcttgttc tcgactttct tgcgatagac atcgagtgac tcctgcacta
     4381 aacgttatca acaagcgttt attgttgttc ataaagaagc gttttgttaa ttcacgttgt
     4441 aatcgccgta ataaatcggt ggccgtgctt agcggcgtat tgggcagcaa aatgagaaat
     4501 tcttcagagc catatcgaat tacgctgtca ccctccctta ttactgtttt tatcacttgg
     4561 accaaaccgg ttaacgtgtc atcgcccgcc tgccggccta attgggcatt taattcactc
     4621 aagttatcga cgtctagcaa cgctaagcat aatggtttac cactttgcga catgtggctt
     4681 tgttctgcac taaacgtttc gactagggcg cgatgactta acgcaccggt aagccgatcg
     4741 tcgcgattat gctcactgat ctgacgcaac tgctgtcgta attcgttgac acgctgctgt
     4801 gcagcatctg cttgttgttg cgtttttact aattcatccc gagcgcggag catgtcgagt
     4861 tgcatatggc gagtatcttc catcagctct tccaatagcg acttaaaatc aacggcctga
     4921 ctcgtttggt gaacacgttc tgtatagtga tctattttgt gctgaaattc acccgttgct
     4981 tctgttacaa cccctagacg ttctaagaat accgaaatca tggttttaag cgtttgctgc
     5041 gcttcagtca aggtatgctg taacagccct tgctgataaa ctagctcctt gagtatcgat
     5101 tctgcgtcat aaatgcggtg gagcgttagt ggctttgcca aatttcgtcg aatgttatca
     5161 agttgatggg ttacccaggg atcgagatcc gatggaagtg atagatgttc tacgaatagt
     5221 gcaagtacgc ttaacaatcc atcgatcaac ctttgttctt gatcgatgcg caacgccata
     5281 tgaatccagc actgtcgaag tcttttctgt atccagtcga tattttcagg ttgttcgatg
     5341 gcttcaatcg cttggatgat gaattcaatt tcatcaatcc aatgctggtg agaagtaagg
     5401 cgaggaatga ggccatcgcg caatgtgtta acaacgagcg cttgccattg ttgccacgga
     5461 tactgggttt gtgaacaatt ttcctgcgcc agactatcca ttggcatgat ttcaacagga
     5521 gaattttcat tatcaaatac ctcaatttca tggggcttat tcagccagtg cgtgattaag
     5581 gtattgagct cgtgattaag tttttcaggt tgaccagcaa atagtgctac ggtatacgct
     5641 aattcatcac gtcgagtatg gtcgtcgctg gcttttccac gtgcgtccca tagcattagc
     5701 agtaattctt cccatggtcg ctctagttgc tgattaggcg atgcttgtaa ttgaatcagc
     5761 tgcccaacca attgagcaac ggtcggccaa ttttcattgc cgactgctaa attaatatca
     5821 accacatagc gatttgctgc ctgaggtaag ctaagcgttc gtagtattgc caataaatta
     5881 ttggctaatg aatccgacac atcatgcttg ggtggattta tgccagcaat ctgataataa
     5941 attgcttgat actgatcggg ggttggcgca atacgctgag tcgctaaatg ctttagcgtt
     6001 tctcgagcga tccagattgg atttttagta tcaggttggc ttgcttccat attctgcgat
     6061 atttaacgcg ataacacggt gaaattttca tttccaaacg atgttagcag caatcgtcaa
     6121 cagcctactt gaaaattacc gcaataaaaa gtaccaaaat tgtttattcg ttaaaccaat
     6181 ttgaagatag cacttagctc aaatttgctg caccctacaa aaattgcgcc tattgtttca
     6241 ttaagggcgc ttatcactat tatggcttta cttaatattg atcgtcacaa atctttcatt
     6301 tttctgtaac attaaattca tcattgattg ctagtctttt tccttcgagt ttttaaacat
     6361 gagggaagta agttatgact agatttagta agttaaatat ccgactaacc gcgatcacag
     6421 ccgctgtaat gagtgcattc gctatcaatg tgcaagcagc aaccgctacc tataatccat
     6481 ctgataatac ggttgattta cctatcgttg aggtgctaga tggtgcaaca tcgaattttt
     6541 tcagtgccaa gctacaactt gtaaatggta atgagcttgt cctagttgat gcacaaccga
     6601 ttgcacccgc aaaaaatgtg caacgcaatg tgtatgattc agcgactact gctgttcata
     6661 tcgcctcggt cgacgcaggg ggtgcggaat tttacgctaa attgaaattg attccaggtt
     6721 caaatccgct gcgcttttca ttagagcagc tcgtgaataa tcagtttgag agttgtccag
     6781 attttgcgac accaggcccg attgctaata gctgtatatt gagtggtgaa atcagaaacc
     6841 gcaatattac gttgactaaa aatacccagt ggattttgtc gggtggtgtt tttattgggg
     6901 gtgataagac ggaaaatgcg acgattacga ttaatcccgg tacaaaaatc gttggccagc
     6961 aaggagcaga ttatctttgg attagacgtg gttctaagat cttagcagaa ggtacgcctg
     7021 ataatcctat tgttttctca ggccccaatg agcaagctgc cggtgaatgg gggggagtcg
     7081 tattaaatgg ctttgcgcgt atcaatggct gtaatgaagg aactgccgta tgtgaagttg
     7141 cgaatgaagc cattacgacc gaattttcag gtggtaataa tgatgctgac agcagcggaa
     7201 tcatgaaata tgtgcaaatt ttgtttgccg gctttgcagt tcggcccgat gaagagctca
     7261 atgggttaac actaaatgca gttggatctg gaaccgtgct tcaattcata caagttcatc
     7321 gtgggctaga tgatggtatc gagctgtttg gtggtactgc aaatctcaag catatcgtgc
     7381 tgagtggcat tgaagatgat agtctggatt gggggctggg ttggagaggt aaggcacaat
     7441 tcgtactgat tcaacaatca gcaagtgatg gtgatcatgg tatcgaagcc gacaataatc
     7501 cgaataatcc agatattgcg cctcgctcaa aacccgtgtt atcaaatatg actctcatcg
     7561 gttctcctgt ttctcaggcg tcaggtgctt tattaagacg tgggacaggc gtgaatattt
     7621 ggaattcaat atttactgga tttcaaaact gcttaacgct tgatggtacg gcaacatctg
     7681 cgttggtcgg tcaagcgagt gaattgagca tcaataattc ctttgtaaac gggtgtcaga
     7741 atagcttcgc agaatcaggt ggcgcaactg catctacagc agactggttt aattcacaaa
     7801 ccgggaataa agcacaggat ccattattaa atggcttctt acctaatgca ggctcaccag
     7861 tgttacaagc aggcgctaca ttgagtgata gctactttaa gccagttagt tatgccggtg
     7921 cgtttaggga cgccaatgat aactggactc aagaatggac tttccctttc taagcgttat
     7981 ggatcgctaa agactagcag attcaattct tactggaaga agcccttact tatgtagcta
     8041 agtaggggct ttttcattag tagatgaaca attcaatttc tcaaagaaag tcactgtttc
     8101 tttggctgct gagctcctac gagagtaatc ctgaaccgtt agggttgcct taaattttct
     8161 ttttcgaatc attacacctt atcaaaaaaa agagcaactc agtggcggta acaagaactt
     8221 cataaaactg acatagcgcc atcacatgat tcgataaaaa tatgtagaga ttcaatgagt
     8281 tttacgactt aatttcttta tatttggagg aatcagtaat gagtgaatcg atcatggcga
     8341 ctgcagagat gctgcaagat tcatttttta gagatagcac cagtagtatc cctgaggtag
     8401 gccagtgtgc tacaacaaag ctcgaaagtc agaccaatat tgtaacgact tgggcaagac
     8461 acttatcgga agtgcgcgaa gctcaaaaat taagatatca agtatttgca actgagatgg
     8521 gtgcaagcct tcctaatatt ttatcaggct atgatatcga tatctttgac gattattgtg
     8581 agcatcttct ggtacgggat cgtaaaacat ggcaagtcat tggaacctat cgcgttttga
     8641 cacctgcaca agctaaatta gctggaaaat tatacagtga tagtgaattt gatttacgtg
     8701 cgttgcaacc gttgcgcgag catcttgcag agatcggccg tagctgcgta cataaggatt
     8761 atcgtcaggg taatgtcatt ctggcacttt ggaaagcact cgttcagttc atgacgcaaa
     8821 ataagctgga tgacattgtc ggatgcgtca gtattcccat gaatatgcag gtaaaggatg
     8881 gtcaaattgg gatgggatat gcagcagcaa gtatttggcg gcagatgaag gaaagtcacc
     8941 tggcaagtca agaattacag gttattccac gtttaccttt accggttgag caattagatc
     9001 agacgctcga tattgatcct cccgcattag tcaaaggcta tctaagggta ggcgccaaag
     9061 tgttaggcgc cccagcttgg gacccggatt tcaatactgc agatttgccg atgctaatgc
     9121 gcatgagaaa tttgcccgaa cgttatcgta aacacttctt ggggtaagta tgcgcgatca
     9181 ttacgcttat ttgcataacc aattcaataa acccactgat aactggcaat caaacagtga
     9241 actggatgac tctccagaag aaaactataa atcaagcgtt cgtgcgattt ttatttctga
     9301 tcttcatctt ggcacaacag gttgccaagc ctcagcattg ttagattttt tgaaaactta
     9361 ctcgagcgag cagttgtatc tggtcggcga tattatcgat ggctggcagt tacgtcggcg
     9421 ttggtattgg cctcaggctc ataacgatgt gatacagaaa ttgctccgta aggtacgcaa
     9481 aggctgcaaa gttatttatg tgccaggtaa tcatgatgaa tttgcacgtg aattcttggg
     9541 acactcgttt ggtggcatcg aaattgtcga ggaggcagtt catgaaacag tcgatggccg
     9601 caaattttgg gtaattcatg gtgattattt tgatggcgtc attcagtgtg caaaatggct
     9661 tgcctacgtc ggtgataatc tctacgaatt tacactgaag ttgaatcgat acttaacctc
     9721 gctgcgagct cgattgggtc taccctattg gtcgctttct cagtacctta aatataaagt
     9781 taaaacagcg gtcaattatg tgctctcctt tgaacaagcc gttgcggccg aagcaagtcg
     9841 acgaggactc aatggtgtga tttgtggaca tatccatcac gctgaaatcc gtgacatcaa
     9901 tggtgttact tattgtaatg atggtgactg ggttgaaagt ctaaccgcat tagtagagca
     9961 ttttgatgga agtatcgagc tgattcactg gcctcagcat gccctgcaac taaccaatac
    10021 ggtagaaatc ccgctaacgg aaggctgctt accgataggt aagcccgtag taacaaataa
    10081 gattaaggat aacgaaaagg tgcttgcatg aagattgctt taattactga tgcctggcag
    10141 ccccaggtca atggggtcgt aaccacttta gtcgaacttg tggcagagct tgaaaaattt
    10201 gatcaccaat ttatgattat tcatccaggc ttatttaata ccagaccttg ccctggttat
    10261 gccggcattg atcttgctat tcggccacga aaacatttgc aaaagctact cctgcaatat
    10321 aatcctgatg caattcatat tgtaacggaa gggcctctag gctgggcagc gcgatcaata
    10381 tgcctcaaag aaggttggtt ttttacttct gcctttcata ctaaatttcc tgaaattttc
    10441 aaggcgtcac tgggtgttcc agtgttcgtg ggatataccg tgctcaagca tttccaccaa
    10501 ccttcctcag gggtaatggt acctactcaa tcggtactag aaggtcttca gaaacgtggg
    10561 ttccaaaacc ttaaatcttg gtcacatggt gtggatactg aattgttccg attccatgag
    10621 aaaccggctg aatttcctgc gttagggaga cttccaagac cgatttcctt atttgtaggt
    10681 cgaatttcat atgaaaagaa tattcaagcg tttctagatt tggatattcc cgggagtaaa
    10741 gtggttagtg gggtagggcc actcgagagt gaccttaaaa gacgctatcc caacgttctt
    10801 tggttaggta ttttacccag aaacgaactg gttcagcttt atgcaagcgc ggatgtattt
    10861 gttttcccga gcgctgctga tacgtttggt cttgttttac tggaatcgat ggcaacgggt
    10921 acgccagtcg cttgctttcc agtggatggg ccgcttgaag tcattggtga tagtggagca
    10981 ggtgccatgt tgcccgattt aaaagaagct gtactcagcg cattcgaaat acctcgagaa
    11041 aaagcacgtg aacgagcaga aacatttagt tgggggtctg ctgcgaagcg gtttgccgat
    11101 tacctggtcc cggtgtaaag gaaatgctac gaatgattgc ttgatagaat ttgctttgga
    11161 ccataataga agcaagtcta tattgtaccg gcgattaggt tatcgttttc tgttgcgccg
    11221 ttatttaccc caatgggtaa gaattagcat cttgcaacag cctattagca cgcagtacga
    11281 gatgcattct gctttagtgt acgagccccc cggtgattcc attcatacca ttattgcttt
    11341 ccctggtttg tctgttcatg ggtacaggga tcatcggatg gatgctgttg ctagagcatt
    11401 ttcatacttg ggttttcgag tgattattcc gtgcgtttcg gatattgaac agcttatcat
    11461 tcacccatcg acgatcagta agtttgcagc gttaattgag gcaattgcga ctcattcgga
    11521 gttgaatcca aaccaccgtc agctaggtat tttctcagct tcatattcag gcggggttgc
    11581 gctattggcc gcttcgcatc cacagatttc atcctccata aaagcgatgt gtttaattgg
    11641 tacatttgca gattttagga atgtaatccg atttgtcctg gatcaggagg aaatcgacga
    11701 atatgcacgc tttattttat tgcggaacct actttctcaa agctcatatc gaaatccaga
    11761 agtaactcag ctatttgata ttgcggtggc agataatggt tttaagaaaa aaaatccatt
    11821 gctaccttat tcgttacaaa atacttctaa agaagcaacg catttttttt gtcaattatt
    11881 ggaagacgcc ttttttaggc atcaagtagc ccaaagtgct ttagaagaaa tcgatcgaag
    11941 agaacattgg ttaaatcgat ttgatttaga taagcagctt acgcatatcg atttttcaat
    12001 tagtttgata catggttatc atgatcaagt gatcccgagt catgagtcaa ttttcttgca
    12061 taagcgctta gcgcagttgg gtaagaaaag tcacttgata ttaacatcgc tactcgatca
    12121 tggagatgtt gtgttaaaca aaaatttctt cgttgaaact gataagctgg ctcaaggatt
    12181 aggttttttt gtgcatgagt tatgcgagag cacaagttaa gggtaaaaat ttttgatatc
    12241 atttgaccac aaggatggag agccttcccc atccttgcct gctccgttag tttactactt
    12301 tatatccata caatttgcat aataagttac cgttgctggc caatctgaaa atattcccaa
    12361 gataccgacc tgcttggtaa gtacatctaa taaaacatat tgatctccat ctttattaac
    12421 aacgctatcg atggtttgat aataccaatc accatttgca attagtggag gggagcgttc
    12481 gatggtccag gtaatgatat ctaagcctgc ttctttggca gctttagcat atactgaagg
    12541 aacaatgcgg ttattttcta ctgtcaccaa tacccacata ggaggagcaa taattttgac
    12601 gccttgtgaa acgagatccg acatactcgg gttccaacta gcagagtttt gaggatcaaa
    12661 agtaggatcg tcgtatcgtc catctagata taccgcttgc gctccaaatt cagattcatt
    12721 ctgaatccaa tagagtacat cacttaaact aaaagactga gggaaaactt tctcgggctt
    12781 aatccctaaa agtttatatt catcgattaa cttttgtgca taatcttgct gtgtaaaatc
    12841 cccttgaaat ggcatgggaa cgctgggtgc tttgagttca ggcgtcattt ttacacctaa
    12901 ttgcttaaaa agttcgatgc tttctcgatg agtaagtagg gtacctttac ttgcatataa
    12961 atcggttctc caagctgggg ttccgcccaa ataggcttca ggagttgtag cagcaggatt
    13021 gcttgcatcc attttaccgt tgagcatttt aaattctgca agtgtgatat cgcttgtgca
    13081 gcattgtgcg gaggccggtt taataatttc tcccgttatt gggtcaatct cagcaggtga
    13141 aaaaggaact gagcatttag cggcaagctc gggaactgcg agaatgttag tcgtcgtgtg
    13201 caggtcacac tgggaatggc gacagactaa ctcacgatcc tgtgtaaacg tcacatcgca
    13261 ttcaataatg ccagcaccca ttcgagccgc tgctaaatag gattctttag tatgttcagg
    13321 gaattgaagg gctgcgccac ggtggccgat tgaaaattcg gtttttctcg atactttatt
    13381 ggcgcatgat tttaatttat tttttaaggc accttcgctc atatcatcaa ccagaaaaaa
    13441 tgggcgaggt cctaattgaa tattggttct tttagtttca ctcactttta cttgatcaat
    13501 gtctttctgg ctgtgatccg atgcagcaag tgaatttttg gttaccatac taaaggtaat
    13561 tgaaaataac atgcaggatg caatgattgc catggttctt tcaaaatcca tgagattctc
    13621 cttttctaaa aagtaaagcg attacaagtt aaaacgaatg gaaacagtgc actgtataaa
    13681 cactttagct ttggtttatg tctgaacaat ttcagctttg ttaatttttt aagaatagag
    13741 ttatctgaaa aggctgtaaa aaaactggat tgaaaaaaca tccattgcta gcgaatgttt
    13801 ttcttcacaa cgattctgta taaagaaact acagaagaag cgttagtggc tccttattgg
    13861 ggaatttaac gaggtaaata agaatatttt ctgagtgata gggtagttat agactacgag
    13921 gcacacaaaa atgtaacctc gtagtcgcgg cactacccaa ttaaaggaat tgattgttgg
    13981 ttagatttta cagtttttga gaaagtctaa ctgttccggc gctattcaag tatgcgccta
    14041 agcgcctagc taaataattc tcttttgtgt tatcagtagt acttagtttg gtttcaaggc
    14101 ctaaaccaca gtcatagagt tggctatgta gaatagcgat tcgagaattg tggttttcgt
    14161 taatagtttt agctttgaaa ttatcataag tgttatttgc cccttttgat gtatcaacta
    14221 ctacggtacc atccggcatt gtaatgacga ctcggccatt tgcaatcgtt gcagctaaac
    14281 cttctgcttc aatgcgtaaa gcactataag ttggttcatc cttagtcgca tttagcttat
    14341 tttttaagcg ggtttggata gccgaagtta aagcagcgtc taaaacagca tagtctggtt
    14401 gtgtgactct agtttgaacc cagggagcac atgcggccat tagattaaag ctagatacca
    14461 gcatgattgt tgttatgacg agaattaacg aaagtttttt catttttaga tctccattta
    14521 atataaagga aaatatctgt acgctacata aacgatcctg tttgtaagaa tcgatgtgga
    14581 tataaattcg aaacaacggt tgaatttata caacgccgat actaaaggct attgatgtca
    14641 gatatatgtc aattacatta aatatttgtt agagagtgtt aggagtaata tcgttttagt
    14701 cgccattttg gttattttag ccaatcaagt tattttcatc gaaatgatat gaagatacga
    14761 agaaaaaaca ggtaccagtg taaagtgagt agtataccca cagagggaga aaccactggt
    14821 acccagagca acagaaggga ggtacttagt acacaccatt atacagttga gtgttactgc
    14881 ttcatgacag tcaagctgat cccttagttt ttttgttacc tcacaagatt gtttctaaat
    14941 tgcttacagc aatctgcgtt gttttttgat taaatcatag ctttcttggc ttgagaccct
    15001 ccactgctac taactgcaac ttttcgatac cgacactttg ataattagtc atcaaatcat
    15061 caaaaaaaat taatcgaggt gttatcaaac tgtcactttt tgaaactaag tttgctgtat
    15121 cgaaaattac attaaaagtg gaggtaatat gagtagcaaa ctgcatcccg atagtaagct
    15181 caaaattaat gacataaccc gtaatgagag taataatcca tcgatgatgg aaatcatcga
    15241 gcaaagaaaa atatcacgac gtgaatttct caagtcatcc gtgactgcaa cggcaggaat
    15301 caccacaatg accctcctgg gaactgcagc gatggaaagt atggcaagtt ttgctgaaga
    15361 tatttctaag catggcctac ttttgggttt taacccagta agccctaatt tgcaaccgat
    15421 gattgatgga ataacagtgc cagagggtta tacagcaaga gtgttgatat cctggggtga
    15481 ttccattaac gataaacctg actgggatac ggtttcagca atggatgagg aaacacagct
    15541 ccattgttat ggggcccata cggatggtat gcattttttc ccctggccag ggcaggctgg
    15601 gaaccagcgt ggattattag tgagtaatag tgaatattgt gaccctccat tagtcaacaa
    15661 tattacccct gcaagcgatt atgccaaagt tccgatcacc cttgcaatgg ttcgtactca
    15721 gcaagctgcg catggtgtca acattatcat gatagaaaag aaacggaaga aatggtcact
    15781 ttcccgtaat tggtatttta accagcgcat cacaggcaac acgccttgtg ttatcagtgg
    15841 tccagcggca gggcatgagc ttatgaagac tgctgcagac gctaccggta aaaatgtact
    15901 gggtacattg aataactgtt cacatggtta taccccttgg ggaacctacc taacctgcga
    15961 agaaaattgg aatggctatt tttctaatga gagtggtgat gtcatcggtg aattagatct
    16021 tgaccgaaaa cttaacattc tcaaaggtca gtctcgttac ggtgtagtaa aaggtggctt
    16081 tggttatcgt tggcacgagg tggacgaacg gttccgtgct gacctacatc ctcatgagcc
    16141 gcatcgtttt ggttgggtgg ttgagatcga tccacgcagc ccttgggata agccaataaa
    16201 gcgcactgca cttgggcgaa tgaaacatga aggtgctcaa gttgtggttg atcggcagaa
    16261 gcacgttgct ttttacatgg gcgatgacga acgcaatgaa tatatttata agtttgtttg
    16321 tgccaaaaaa tggaatggca atccgcaggt gaccgatccc gatatgctcg ataaaggcac
    16381 gctgtatgtt gcgagattta acgccgatgg cggtggtgac tggatgccgc tgatctgggg
    16441 tcaaaatggg ttgactcctg agaatggttt tagcagtcaa gcagaagtgt tgattaaaac
    16501 gcgtcaggca gcagatcgcc tgggtgccac gatgatggat cgacctgaat ggggtgcggc
    16561 acatccgaag actggcgaag tctatatgac actgaccaat aacagccgcc gcggcaatac
    16621 tcctgcgtcg agtaatcaaa ttgatggtac gagcaatgct ggtagcgcta atccttcggt
    16681 agatgctgct aatccacgcc cagataatga ctatggccat ataattcgtt ggcgcgaaga
    16741 tgatgcaaat gttaccgcaa cgcacttttc ttgggatatt tttgtgcaat gtggtgatag
    16801 agactccgcc aaagctctgg gagcgagtta caaccctgcg ggctctgatg gttataaagg
    16861 gaatattaac ggtgatgact acggtgcgcc agatggtttg tggttcgata aagacggtcg
    16921 cttatggatt caaaccgatc aacaaggtga tgcaaaaggc gattggatca atatcggcgg
    16981 taatgtcatg atgtgtgcta atccacaaac cggtgagacc aggcgtttct taaccagccc
    17041 accaaactgt gaagtaacgg gtgtggtgac tacccctgat ggccgtagca tgtttgttgg
    17101 tatccaacat cccggtgaag attggaagat caactttact gataattcaa gctggccgga
    17161 caacggatac aacggcccta ccacttttac cggcgctgtc gtatctaaac cacgttcatc
    17221 ggtgatcgtc atcactaaag atgatggtgg agttattggt acttaagcac tgctacatct
    17281 aatcgttcaa atagctgggg ctcataattt ctgacttcat catgagcttc agtatcatct
    17341 agtgctacta agattcagaa tccatatctg gtatattcaa ttcactctag ccgaattaca
    17401 ataaaacgag attcttatct gatttttcaa agacctctat atacttctgg cttcagatga
    17461 tttaagtaac tatcctaaat gtacactaca aattttgatt atcttgggct gatcaatgac
    17521 tgccgacaac aataatccga ggctagctgt attgattgac gcagataaca cctttaaggt
    17581 gatcgatctc attgatgaat tattcgagga aatatcaaaa ttcggcgatg ccagtgtcag
    17641 gcgtatttat ggtgattgga ctcaaaacca gttgagtcga tggaaggaaa tcttgcccaa
    17701 acatgccatc cagccaattc agcaatatgc caataccaaa ggtaagaatg caacggatag
    17761 cgcattgatc attgatgcca tggatttact ctatacggcc ccgctggaag gtttttgtat
    17821 cgtttccagt gatagcgatt tcactaggct tgcaattcga ttccgagaat ctgggaaact
    17881 tgtctacggt tttggtgagg aaaagacacc ggaatcactg caagctgctt gcaatgaatt
    17941 caagtacttc gaaattctct cgcaaaacaa gcatatcgga attggtgata gtgttgcgac
    18001 acaagcggaa gatattccta acaaatccaa aacaagccta aaaaagaaaa ctactaaatc
    18061 agtatcattt tcgagcgaag ttacaaccat acctggcacc aaaaaaaatt ccagggaact
    18121 ggcaatggat accaaactgg tatcgctgat ccgctccgca attgaagcaa tttccgatga
    18181 agatggtttt gcttacatgg gagaagtgaa gaaacatatt gtgaaaaact tctcggcatt
    18241 tgattcgcga aattatggtt acgcaaaatt cagtgaccta attaaagcca ttggtttgtt
    18301 cgaagtagac acccagaacc agcatattaa ggacaaaagg aaaaaatagg cgaattttac
    18361 gcgccaaatt cattagcaac tgttttcata gttatagttt cgtctatcag ataaactact
    18421 gtaaacagag gaaagtcttg caataccgca tttgttattt cttttgtttt cactagatct
    18481 agtgattctt ggaggcctta ttattactaa tttgttcctt atatcattca gaattgaatt
    18541 agttaggctc tagactaggt caatcctaaa atgaggtaag acgggtacca gtgcggatgc
    18601 gaatatgaaa cagacagggg aaaagcaccg gtacccagac caagaaaagg gaagcacttg
    18661 gtagttatca ttatacggtt gaatattacc gcttcatgac agattggtag attttcaagc
    18721 gatcttagcc gtcccagcaa tttctctaaa tcatagcgac tgatcattca ttcttatccc
    18781 tgattaaact ccctttctgg cgtgattgac ataaaaatgt aacattcgcc cagtacgata
    18841 ctagcaattc taagtagctt gttggaccac attcataacg gaaataatcg atttggcgct
    18901 gccaatactg agcagaccat tatcgatttt ctgctaccaa gtgaaacaat cccagttgag
    18961 gtttttttgc aatgttatat ctattgaaaa aatgttttga gggactgctg acgttaattg
    19021 ggctgagtat cattacgttg tatgcaccca ccgttactgc agacaacttg gagaatttag
    19081 cgaaatcact cgcaaaaatt cgcggtgaag tcgaggagtt gcaatctcag ttggatctag
    19141 agaaagaaaa ccataatagt cggatcgctt ctctctcatc tcaattagca gatttgagcg
    19201 tagaggaaag acggcagaaa atcagcatag aaaaactgca gcaatcgatt gagaaattaa
    19261 ctgcagaatc taagcaggcc gaacattctg gtgataattt aaagccaagc ttgattctat
    19321 ttttggctga atataagcga ttcatacaag caggcttgcc atttaagatt gaagaccgac
    19381 ttaaagccat tcaagagcta gaagacaatc tcaacaataa gcttatcgat cctaaaaagg
    19441 cggtcaatca tgcttggtca ttgctggaag atgaggtgcg gttaagtaaa gagaatggta
    19501 tttatcagca aacgatcgag ttaaacggtg aaaagatact ggcggatatt gccaaattgg
    19561 gaaccatttt tctttatttc caaacgcgtg atgggcggtt tggtatggcg cagcacactg
    19621 aaggatcgag ttggaagttt gtttcaatgg atactaagaa agataaaact caaatcagtc
    19681 agcttttcga tgcattaaaa aaacaaatac gccaaggtta ctttgagtta ccgaatcctt
    19741 tgaaaaaatt atgaaaccat ttcttttagt cgggttgttt tgttatttga tttccacagc
    19801 ttgggcgcag gaaacgacgc ctgaagtgtc gcaagcaacg gaaacaatta ctactgaacc
    19861 tgcttcggat aaaaaaacga ataccaataa tccaggcaag gcagcatcag ctaagaaaaa
    19921 cacaacctca gattcgaaac ctgttaacct cgaaactgcg tataaaaggg agtatgcgtt
    19981 tctggatgcg cagaagcgag aattgaaagc gcagctggat aaatacaaag ccagcgctaa
    20041 tagcaaagaa caagagttga ttcgaaaaat taatgcgctg gaacgtgaat ctgtttcacg
    20101 ctcggcaaaa atcgatcagt tcaatagcca aattgtagaa gctgagcgga ctcaggcggc
    20161 ggtgagtgag cgcaacgatg ctttggaaat aacctatgct cagtcggagc tgaccttgaa
    20221 gcagtatggg attgagatgc cccctccaat taaggaagga aaggaaaatg atctagaaaa
    20281 gactactttc ttactccagc aaggtcttgc gctgattcag aaattaggag aaatacaatc
    20341 taaggcaggg aagttctttt tagaatccgg taagcaagca gaaggttcta ttatccagtt
    20401 aggcaatatt gctgcatttg gtgtcagtgc ggatgggggt ggagccctgg ttcccgctgg
    20461 aaatggtgag ttcaaagtat ggaaagaacc tgcagcagag attgcaaccg ccttgaataa
    20521 caatgagcag ccggattatt tgaagctatt tttgtttgaa tcccgtacca ctgcgattga
    20581 cgaaacccct gataaaacag tgctaagcat tattgaatca ggtggtttga ttggatgggt
    20641 tatcgttggc ctgggcgctc tcgcactttt attggctttc attcgcgctt ttttgttacg
    20701 aaaaaatagt acggataata agcaactagc cgaccgaatt attgcactta tcgctgaggg
    20761 ccgtttggct gaagccaaaa agatatgtga gagtggcgga tctgccattt ctcgtgtttt
    20821 agcctccacg cttcgtcatc ttaaagatga tagagagcat atggaagata ttgtgcatga
    20881 agccattctg caagaagcgg gtccattaga tcggtttggt tcggcgatta tggtcattgc
    20941 ttcggttgca ccgctgctcg gtttattagg tacagtcacg ggaatgatta caacatttga
    21001 tgtcatcact gaatttggaa caggcgatcc caaattactt tcaggtggga tttcaattgc
    21061 tttagtgacg accgaattgg gattgatcgt agcaattcca acactaatgc ttggttcctt
    21121 gcttaatgct tgggcaagga atattcgacg tgatatcgaa cactctgcat taagggcaac
    21181 caatgccttt ctaggtggaa aatcgggtaa ttcagtcgcg acagatcctg gggctgggaa
    21241 tgatcctgaa actcagtcat ttagattgca accacagcca atctaaccgc tggaataagg
    21301 ttggcgcatg acattgagtg agattgttag tcttgttgaa gaattatttc aagccggagg
    21361 gctggtcatg ccgcccttgt tttgctgtgt gttattactg tggtacggcc ttggttatcg
    21421 cttctgggtt ttaaaaacac cccaaaaaat gggtgtaagg gacttactca gtttttatcg
    21481 tgagggaaag tttcgggatg cgcaaagtat tgtcgagcag gccattcaaa agggcgttgc
    21541 gctaaaaaaa aagcagagcc agtatctctc ttatcagctt gacgcagctt tttatgagtt
    21601 tgagcgtgag atcgcaagat tttctgtttt agtcaaaatc atcgtaatta tttctccctt
    21661 gttaggcttg ttaggaaccg taatcggaat ggttgaaaca tttgattcgt tggctagtat
    21721 ggctttattt actcaaacgg gcggtatagc cggtggtatt tcgcaagcct tgtttacaac
    21781 ccagatgggt ttggcagtgg cgattccagg gttgctcgca catagttttt tgagcaggaa
    21841 acaacagcaa atcgagaagg atttgcttca agttaaaaaa ttgttatgta ggttgactta
    21901 ataagataga tattctgaac aagattacat tatgagatat agcgaaccgc ctgaaactga
    21961 gacagcgatt gatatggcac cgttgatcga cattgtattc attttgttga ttttttttat
    22021 ggttaccaca acctttgtca aagatatgca attagagcta gaacgaccta aagcgagtag
    22081 cgcagtttcg tcttctagta aagccattcg attattcatc gatcgcaatg gcgatactta
    22141 tatggatggt gagccagtaa gaatctggct tattcagagc aaactcagag atttattgag
    22201 cacttcaaca aataaagtgg tgctagtcgt tacggatgaa ggtgttccag ccggtaagtt
    22261 ggttgaagtt gttgatcaag cacgcttggc tggggctcag agtgtcggtg tggctacacg
    22321 caaagaagct ggataaataa aaaatgaatg gggtatttgc atgataaaaa aaccaatgaa
    22381 taagcatatt gcagcgacga tatcgatgct gatggggcca attttagtct tcggtatggt
    22441 gattctcatg aataaatttg cagattcatt agataaaacg cctgcgcaag aagttaccga
    22501 gatcagtatg gtaaagcagc ctccaccgga gccaaagcaa gagatcaaga aagtggaacc
    22561 taaaaaacaa ccggttcgtt ctaatacccc tcctcccttt aagggtctag gttcttcttt
    22621 gtcaggaatc gatctgggtt tattgggttt cgataatggc ggtatgactg gcgttgatga
    22681 gagtttgctt ggtaaaaccg gtaatgccgt tatgacagaa gatcttgtcg atgttcctcc
    22741 aaaacctaaa tcacgtagtt cttttcgcta tcctcctgca gcaaaaaaga atgggataaa
    22801 aggttatgtt gttctttctg ttttagtaga taccgatggg tcggtcaaac aagtacaagt
    22861 gctggagtct aatccaagcg gtgttttcga cgatgcggct ttgcaagggg ttcggtcgtg
    22921 gcaatttgaa cccgccaagt ataaagggga ttccgttagg gtttgggcta agcagaaaat
    22981 tcgctttgat ttatcttaag aatagaaaac taggtgtttg atgatgctca tagctacaaa
    23041 gcgagccata atcgttgcga tcgttctgat tagtggttat ttccagccag ccttggcgaa
    23101 ggaagaaaaa ccacaatctg atgattttat cgagttagca gctatcatgc ttaaggatgg
    23161 ccactatgat cgcgcattac tagcactgca aagcgtcaat ctagatgacg aaaaaaccga
    23221 cttagcgcgc ttttatacct tgcagggttt ggcctacatg aatctcaatg attttgacgc
    23281 atccaaagat agttttcaga ctgcgatcaa gcatgggcag aaagatccac ttatttatgc
    23341 ttatctagcc caaattcatt tttcacttaa ggattatcaa ggtgtcctca acgtcattga
    23401 taaaacaggt gaacataaaa gtaagaaccc cgcgttgaca tcgatccagg cacagtccta
    23461 ttggcattta caaaaaactg tgcaagccat cgaaacactg aataaaggcc atcagctttt
    23521 tccaagtgat tatcgttttt tgaaattgaa agttttttat ttcgtgcagc ttcagttgta
    23581 tcaggaagct gctaatctag gccgagaata tcttgctcgc tcgaaagccc aagcagaaga
    23641 atatgtggcg attggtaatg cattaaggct cagtcgagaa tttcaggagg cccttaaaat
    23701 catggaaata gcacggctga aatttccaga taacgaggta gtcgcaaaat tgcttgcaca
    23761 tatttatctg gatcaaggca tgctgaatgc agcagcacat atccttgagc aggcggcacg
    23821 tatcaattca gatttattgt ctgaggctgc agaagtttac cgtcgtgcgg gacgtttgca
    23881 taaggctttg atgctcaatt caggaatcaa tgatcaaaaa gttaaactca agcaacgatt
    23941 atcgattctg ctcgccctta aacagtatga tcaggcagcc agtatggaag cgagtttata
    24001 tcgtgtcggc ttgtttgatg atcagagtat tcgttatgcg cttgcctatg cgcagttttc
    24061 cagcggacga tttcagcaag ccagcaagca cctcgatttc ttaacagaac ccgagttatt
    24121 caaaaaaggc attgaattac gtcgtctgat ggaagtctgt aaagaagagc cttggaaatg
    24181 cgcttaagtt aggtagttac ttagcttcgc aatcgtgtat tggcaaagaa tcggtttata
    24241 cctgacaccg taatcgcggt cattcatctc caccacgctg ctaagccagc attcttattt
    24301 ctgtcacatt aatttaacaa tccatcagta gtattggctt gctttaagta ggtcagtgcg
    24361 atttgccctg tctactctcg catcagctaa atctcctttt gcatagataa attccggctt
    24421 ttaaccctaa tagaccactg tactcgcagt aatttaataa aaagaataac gattgaagat
    24481 gatccagctt attcaggttt taatgtttgt aatggctttg gttataacaa caaatgaaac
    24541 atttgcaagt accgaatccg cacaattttc gataaattta ttccaagatg gtttaccaat
    24601 ctcaggtgct gaggtatcag tgataagcga tcaatttggt cgtcctgatg ctcgttttat
    24661 tgatagttcg gcaacatcct atgcatggct tgctaaagac ggttccccga ttacgacaag
    24721 tgccaatggg agtattgcgg gtaagttacc accaggaatc tatcaaatca gaattcgtac
    24781 tcaaagagat caatttttct cttttgaatt gcccttacgt ccaacagaag atgtacaaat
    24841 tttagtcacg ttttatgatg atcctgaaaa aacattattg aatatcgaaa gctcagaggc
    24901 gggcgttctc gcaggcactg agaaggcacc gattactgct gaatcatctg gtgagggtac
    24961 gatcgttgca caagtcgttt cagctgagac acaaaaaccc atcaaggatg tgcaattatt
    25021 tattagtggt ttgaatcaga ggctcagaac cgatgatcaa ggccaagtgc aaatcaatat
    25081 tccgattggt gcgtatagcg tctcattatt acattccgct tataacagcc aaacccgtga
    25141 tagtgtagaa atcaataagg atcagctaac cgaattaaaa tttagcctga caccggctgg
    25201 agtggagctt gcagaatatg tggtgttgga accgcatctt gccgggagtt tgactgctgt
    25261 gattgaagaa caaaagtcat ccgctgaagt tgcaacgatc atgagtgctg aacagttcag
    25321 ccggagtggt gatggcgatg cggcttcagc gctccgaaga gcatctgggt taaccttggt
    25381 gggcggtcaa tttatcttta ttcgtgggct gggagaacgt ttttcttcga cgcttgttaa
    25441 tggcgcggcg attccgagcc ctgatcccac acgacgcgtg gtgccactgg atatttttcc
    25501 tacgagcgta ttagaaagtg ttttagttca aaaaacttat tcgccagatc gacccgctga
    25561 atttgcggga ggtaccattg agctccgcac gcgaggtatc ccggaccaat ttttcttcaa
    25621 ttttaatgga tcgattggtg ttatcgataa ttcgaccttc gcaaaaggat taacctacaa
    25681 agggggagat ctgagttttc taggctatga tgatggaacg cgtgcattgc cgaactccct
    25741 cgcagatatt gtcaaagatg gcggtaaatt ggagcctaaa acgcccttca atccgaatgg
    25801 gcaaacgccg gctgaattag agcaaatagg cgaagggctt gcaacggatt gggatatcaa
    25861 acgcaaggat aatcctccgc tcggtaatat gcaggcggcg atgggggatg tatttaactt
    25921 aggtaatttt agggcaggtt atattgcttc ggcagggtgg aaccaagaaa ttcgcaatca
    25981 aaatgaagtg agtaaactgt ttgtagcgac gcagcgaaac gatgaccaat tactcaaagt
    26041 acaagacttt gatgttaaaa gaacattgcg cgaagttcag ctcaatggtt attttggtgc
    26101 ggatgtcgag tataaggata agcatcgatt atttactaaa actatgttct tgagacagtc
    26161 cgttgatgaa ggaagaattt cagaagggtt cactgataac aatccgactg aaatacgaag
    26221 aacgaggcta cggctatttt ccaatcaatt attgatgcag caagtgggtg gctcccatac
    26281 gttggattgg gcaaaagatt taaaaattga ttggttatat tcccattcaa cggctaatcg
    26341 tgaagaaccg aaatttaggg aatatcgtta tgatcagcta ctgagtaatg atcagatatt
    26401 tgctttttct cgacaaccgg atagtaatca aaccatgtat ctcgatttaa aagataaaga
    26461 tcaaagttgg cgagttgatg caaaacttcc agtagaattt tcacctaatt atcggttctc
    26521 actggaaagt ggaatgatta cccagagtcg aacccgtcat tcgggaattc aacgctatgc
    26581 ttttttcccc tttggacccg atgcaagaaa ccaaacgatt ttgaatcaac cttctttgga
    26641 agacattctg aatcctcggt atattggtcc caatggtttt caggttcgcg atattacacg
    26701 accaaccgat agtttccggg ccaatcagga cttgctttcc tactatggaa aagtggatgc
    26761 taatctttat gagaagctac gaatttcagg ggggctacgc tgggaagata atttaatgtt
    26821 ggtcgaaacc ttccagtcag tgggtaacca gaataggccg atccagtcaa aattagaccg
    26881 tgtcgatatg ttgccatccg tatcagcaac ttggtcagtt tctgaacgac agcagttacg
    26941 ggctggttac agtcaaacga tttccaggcc agacttcagg gagctatctc ctgcaccatt
    27001 taccgatcaa aataccaatc gcgagacgat aggtaatccc aatcttaaac aaaccagtat
    27061 caccaattat gatttgcggt gggagtatta tttgtccccc aatgagaatg tgttggccgg
    27121 ttttttctgg aaagatctca agttaccggt tgaggtggtt aacgtaccgg gtaatgctgg
    27181 attattaact ttccagaatg ctgatgatgc acgggtatat gggtttgaag tggaggggtt
    27241 aaaaaatctt gaattcatcc acacacaact gagaaatttt acagtaggcg ctaattacac
    27301 gtggtccgac tccttggtca atctcaatga aaaaagtctt gaggtgcaaa ccaatgcgag
    27361 cagaccatta caaggacatt cccgccatat cattaatttc cagttaggct acgataatcg
    27421 agaatggggt acccaagcca ccttactcta taatgtcgcg agtcaacgtg ttgaatcggt
    27481 tggtgtgtta ggcgcccctg atcgatttga gcaacctttt catatgcttg atttcgttgt
    27541 tggtcagaat gttaataaat ggctttcttt gcgactcaat atgagaaact tgcttgatga
    27601 tgattttctt gttaagcaag gtgatgaggt tgtacgccag ttccgaagag ggcgagaatt
    27661 ttctctgggt attcgcatta atttctaatc taactactat cttcttctgc taattcgccc
    27721 atcaatatgt gcgtttaagt aaatatcccg tacttggtat tgtcgatttg tatttagctt
    27781 tcaaggatgg attgtgaaaa tcaatggctg gaaatatttt gcggacattg ctttatcttc
    27841 aaattgttct gtatacgcta aaacgttcct catacttatc aaggcttgct aaaaagcaag
    27901 aagcttgaat ttctccttac tccactaaag ttataaaatc acgataactg agtcagaata
    27961 gctcattcat tgctgatttc aagcaagaaa aataatttta tgattcatct tctattcagg
    28021 ttggttttaa tagtatgttc tatatataaa aaaaacagcg atgcgctttt ttcagtggtt
    28081 taaaaagtgt ggctctgtcg aaaattaaat gttgctattg aaaaattgat tatgtccaag
    28141 aggctaaaat gaaaaaattc caattaacgt gcttagcgat tgctatgttg ggattaatga
    28201 caacaaactc agtcaatgct gagggtgatg gtggtggctt cgannnnnnn nnnnnnnnnn
    28261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28321 nnnnnnnnnn nnnnnnnnnn nnntgggatt aatgacaaca aactcagtca atgctgaggg
    28381 tgatggtggt ggcttcgacg gtggtggctt cgacggtggt ggcttcgatg gcggcgattt
    28441 cgatggtggt ggcttcgacg gtggtgattt cgacggtggt gatttcgatg gcggcgattt
    28501 cgatggcggc gatttcgatg gcggcgattt cgacggtggc gatttcgata gcggcgattt
    28561 tgacggcgct aatgagtctc ctgaaactga tggatcgaca ttaggcgaat caatcgagga
    28621 taattcacct gctgatgcta gctcaacttc tgcagatgct gtcgagcaac cagctcagga
    28681 atcaccagaa gcgggtgatt catctgctac aaatgatcaa tcacaagaat cgagtgaaac
    28741 agttgatcaa ggaagtgatg aagccaaaac tgaatcaaca ggacagactg cagatacagc
    28801 ggataatact gcgactgatc aatcgactga caacagtgcg agcaatactc aaactgaacc
    28861 tggaacacag gccggtgatg cgaatcaatc agatcaatct gcttcaggcg gagatagtag
    28921 tcaaaccgat caaacgaatc aagataacgc gaattcaacg gataatagca atcaaaatgt
    28981 taatattcaa aacaatatta atactgacca gaattcagga catcatcaca acgattgggg
    29041 gttaaatcat ggacatcacc atcatcacga tggatttgga ctcggacttg gagttgggtt
    29101 gggattaggc ttgggatttg gtccgttctc accgtttgcc cctttcggtg gatttgggta
    29161 ctatggcttt ggttatccat ttggtggatt tggttattac aatagttttg gtggcgttgg
    29221 tttctataat agctttgttg gattttctcc tttcaaccgt tttgggtatc cttatggtgg
    29281 tttttcagcg tttgcaccgg ctgttccatt agtttcatcg cccatggtga tgacgccagc
    29341 gagaaaacca acttatattc aacaaaggag ctatacacgt agcgcccccg cacctaaaac
    29401 aaactattgg cattattgtc gtaatccaga agggtattat ccttacgtta aaaaatgtcc
    29461 ggggggatgg ttaaaagttt tgccccaacc tgcttcataa tcaaattttc aaaggaaaat
    29521 aggtcatgtt gaaattcgcc aaatcggtaa tgttatgctc aactgccttg cttgtggctt
    29581 gtgtgcatat tccgtcggga cccagtgttt tggtattgcc tggatcaggt aaaacttttg
    29641 agcaatttcg caatgatgac tatctatgta agcaatatgc tgctgagcaa gtcagtggac
    29701 ggacaccacg gcaagctgca attaccagtg gtatggagag tgctgcactg ggtgctgcac
    29761 taggtgcagc cgctggagta gcgattggcg gaggggaagg tgctgcaatt ggtgcgggaa
    29821 ccgggttgtt agccggtgcg ttaggtggta gtagctcagc tcaaacttct ggatacttag
    29881 gacagcaacg gtacgatatg ggttacttgc agtgtatgta tgcaaatggt caccgtattc
    29941 cttcgccggg tcgttttgcg acagaatata cggatggcgg tagtaatcgc tatccaccac
    30001 caccgccacc acgcaccggt tctggaaaat ttcaaccgcc tcctcccccg cctggtaatc
    30061 caccaccgcc acctcctggc tattaagttt ggggaaatta gcgttattaa attttagggg
    30121 agaaaagatg aaaagtttat tattagtatg ttctgcaata ttaggtttat ctttgatgtc
    30181 ttttagtctg gctgatccta tgtcagtttt caaagggcgc gcgattttta ttgcggctga
    30241 taatcaactc accgttttac cggattcgcc tgtccaagcg ttatcatgtc gctttgagaa
    30301 tggaattttt gtagctggtt catcacctag tattgcaatt cctattccag gtgctattga
    30361 tgtaaaaata attggcactc acgcctatgt tgctacttca agcaccgaaa atagttcagc
    30421 tacaacgggt tatgctaaat atgatgtcac agcatgccta cctgatgggc cttttactcc
    30481 gtatcgagca cgtgcgaatc taacgtcggg cgagcttgtg ataccgtgtg tcgaagtcag
    30541 tggcagtgag tataatgtgg ttatgaaaca acgtggtaaa tcgtccaatt gggaagtgga
    30601 ttttgttggt acagggtgca attaacgagc aaaatatttt taacaaagaa ttaccttgga
    30661 ctgtcatcta gtcaatcgtc taaggtcatt caaccattta actaggttag tttctcctta
    30721 gccttgggca atttattcat ggttttttaa ataactatcc caatagggag atccttcaaa
    30781 ccgtttgatt agaaaatcaa taaatgctct gacacgttgc gaaaggtgtc gggtttgtgg
    30841 atagattgca tagactgcca gtgaggaata gtggtattta tcgagaatcg gtactaaagc
    30901 acctttttca atttcctggt acgcaatgaa tgtggggatc aatgcaatcc ctaacccatt
    30961 aactgcagca tcacgaagaa attcaccatt gcttgctttt agataaggcg ttatttttac
    31021 ttttgttact tgatcttccg catcgcgcag gctccaatat tcaaaattac tagtcaagtt
    31081 atatacaaga cactggtgat gggcaagtgc tgccggtgaa tctggtctac cgttccgatc
    31141 aagataagct gggctggcac acaaaattac attgatgggc gcaattcgcc gtgcgatcaa
    31201 acttgaatcc ggtaaattgg cgatacggat tgctaaatca aaaccctctg ccagcaaatc
    31261 tacttgtcga tcattaaaat ctaaatcaaa tctaatctcc gagtggattt cgagaaatac
    31321 attgatcgca ggccctaggt gcatcaaacc aaagctgagt ggtaacgcaa cttttaaact
    31381 accctttaaa gcgccatgaa attgggatgt agcatgttct gcttcgataa gatcagcaag
    31441 aatacgaacc gattgttggt aaaaagcgcg cccactatcc gtcagattca ttcgtcgggt
    31501 ggtacggtga aacaattcaa ccccaaggtg cgcttcgagt tctttcaaac gccggctgat
    31561 tactgatttt gcgatattca tccgagttgc tgcggcgctg atgccacctg cttctacaac
    31621 gctgataaac atactcatat tttcaaaacg atccataaaa tggttttatt gttgcttatt
    31681 taagaaatat taattctatt accgagtctt tattcttatt ttaacaacga taataatact
    31741 tcctgacaat attttataaa tcattgatat aaggagagcg cagcatgaat aaattaagtg
    31801 aagcttctcg tatcattcct gcaacgagca tacgagaagg ggcaggcgtg attgtaaatc
    31861 gcacgatcgg tacaccgatg ttgcgtcatt atgatccatt tctattgctg gatcatatta
    31921 gtagcgatca accccaggac taccttgctg gattcccagc tcatccgcat cgaggattta
    31981 gtacctttac ttatatgatt gatgggcata tgacgcatca tgacagtatg ggtaatcacg
    32041 gagagcttga tccaggatct gcacaatgga tgaaagcagc tagcggtgtt attcattcag
    32101 aaatgccaaa acagcaattg ggtttgatgc gcggttttca gctgtggatc aatttgccag
    32161 cagcccataa gatggatcgc ccagaatata gggagtatag cgagaagatg tttccggtat
    32221 taaggtttcc agaatacacg gtgaaagtac tcgttggcca ctttgaaggg gtcaatgcgc
    32281 ctattatcga ccccgttact gaggtaacgt atcttgatgt gcaattacaa ccggataaag
    32341 cttttcgata tgacctgcca aacgacaata gtagctttat ttatgttttt gaagggtatg
    32401 gtcaacttgc tagtcaagca gtacagcgcc attctcttgt gatattagat gcatcggata
    32461 ataaccccat tacatttctt gcaggaaata aaggagcacg ctttattgtg gtgagcggca
    32521 aacccattca cgaaccaatc gtacggtatg gtccttttgt gatgagcacc aaagaacaaa
    32581 tcgatcaggc aatgaaagat tttcagtcca atcagtttgt ccgtgaacgt gcttgggtaa
    32641 accaaaatca ttagatgatt aatattctaa atgcctatga actattctca gtgtttttca
    32701 tcttatatag caagatcaat
//
LOCUS       DAOQKQ010000003        31484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_555_length_31484_cov_5.990296, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000003 DAOQKQ010000000
VERSION     DAOQKQ010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 31484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31484
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_555_length_31484_cov_5.990296"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1213)
                     /locus_tag="PKZ49_00335"
     CDS             complement(<1..1213)
                     /locus_tag="PKZ49_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HND35034.1"
                     /translation="MPNHLIHETSPYLLQHVDNPVEWHPWNKEALELAKKLNKPILLS
                     IGYSACHWCHVMANESFQDAEVAAAMNQYFVNIKVDREERPDIDQIYQTAHHMINQRH
                     GGWPLTMFLTPEQKPFFGGTYFPKVSRHGMPGFLELLPKIAETYRTRTDDIEKQNTAL
                     LKLLADTLPTTGAKIPVISRQLILRALTQLDENFDREYGGFGQAPKFLHPAELLFCLH
                     CYFYDSDAGALRIVKHTLEKMAKGGIYDQLGGGFFRYSTDQYWRIPHFEKMLYDNGPL
                     LQLYTDIWLATADPLYKQIVEETASWAIREMQSSYKQGEGGFYSSLDADSEHEEGKFY
                     VWSKEEIERGLSADEFSVVNPYFGFTRFPNFESKYWHLEVSQSVTSVAQQTNCSEDNV
                     QRRVDSARDKLL"
     gene            complement(1215..2888)
                     /gene="ilvD"
                     /locus_tag="PKZ49_00340"
     CDS             complement(1215..2888)
                     /gene="ilvD"
                     /locus_tag="PKZ49_00340"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HND35035.1"
                     /translation="MPDNKRSQNITQGAKRAPNRAMLRAVGFSDGDFDKPIVGVANGY
                     STITPCNMGLNDLSLSAERALKRAGAMPQMFGTITVSDGISMGTEGMKYSLVSREVIA
                     DSIETAVQAESMDGVIAIGGCDKNMPGALIALARMNIPAIFVYGGTIKPGHYKGQDLT
                     IVSAFEAVGQFTAHKIDAKELLEVERHACPGAGSCGGMFTANTMSSAIEAMGMSLPYS
                     STMAAEDEEKKVSAAQSAEVLVQAIKKGIHPRDLITRKSLENAISVVMAVGGSTNAVL
                     HLLAIAHAAEVELDINDFEVIRARVPVICDLKPSGQYVTADLHKAGGIPQVMKMLLNN
                     GLLHGDCLTISGETIEQVLHNVPDQPPQNQKIIRQWGDPLYEQGHLAILKGNLSPEGA
                     VAKISGVKNPKITGPARVFDSEESCMSAILAREIHAGDVVVIRYEGPKGGPGMREMLS
                     PTSALIGEGLGDSVGLITDGRFSGGTYGMVVGHVSPEAFVGGVIALVREGDSITIDAT
                     QRLLQLNISEDELEKRRAAWQPPKARYHRGVLAKYAKLVSTASRGAITD"
     gene            3004..3801
                     /gene="lgt"
                     /locus_tag="PKZ49_00345"
     CDS             3004..3801
                     /gene="lgt"
                     /locus_tag="PKZ49_00345"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HND35036.1"
                     /translation="MLIHPQFNPVALSIGPLSIHWYGLMYLIGFAFFILLGRYRIKQF
                     NQSGITFTILDDALFYGVLGVILGGRVGHVLFYQFSYYLAHPLEIFAIWQGGMSFHGG
                     LIGVLLAMFFLARKYQLKWLAVTDFIAPLVPIGLGAGRIGNFINGELWGRPTEVTWGM
                     IFPHVDHIPRHPSQLYQFALEGVVLFILLWVYSNKPRSVGAVSGLFLIGYGLFRSFSE
                     FFRQPDDGFMGQLTLGISMGQWLSLPMIIGGIFLLIRSSRLHVPAHR"
     gene            complement(3898..4185)
                     /locus_tag="PKZ49_00350"
     CDS             complement(3898..4185)
                     /locus_tag="PKZ49_00350"
                     /inference="COORDINATES: protein motif:HMM:NF040598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpp/OprI family alanine-zipper lipoprotein"
                     /protein_id="HND35037.1"
                     /translation="MNRNVKNALTIVALGATVALTGCATTAQFDELKAEVNAIKSDIS
                     SAKADAAAARAAADNANRVAAEARDIANQANARSIEAETRIDRAFKKSMYK"
     gene            complement(4453..5397)
                     /locus_tag="PKZ49_00355"
     CDS             complement(4453..5397)
                     /locus_tag="PKZ49_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HND35038.1"
                     /translation="MSRVMFGVVVFIFSTFAQSASWILPPGDVDLFGQIKTIPANRDE
                     TLLDVARRFGIGQDEMMLANPTVDRWLPEEGAEIVLPKRYIIPQGDRTGFIINLPEMR
                     LYYFPKPNKGKKPVVITHPISIGRMDWRTPLGKTSVVRKQKDPTWIPPQSIKDEAIAA
                     GDPPPPPIVLPGPNNPLGRHAIYLGTAGYLIHGTDKPFGIGMRVTHGCLRMYPEDIET
                     LFEKVTVGTPVQLLNQPIKIGWLADSLFVELHPPLDEDEAEYVDYGQKVRDVIASFIE
                     KTTTGKNANHTRKNIEIDQSALELAIFERNGVPILISK"
     gene            complement(5565..6110)
                     /locus_tag="PKZ49_00360"
     CDS             complement(5565..6110)
                     /locus_tag="PKZ49_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HND35039.1"
                     /translation="MYNKFILLILGILSSCLVNAHGDVWVDVDTNNLTLSVMRGEKIE
                     LSFDNISIGRYGASKARMMGDNRTPIGTFRIGWIKESRRFHRFFVFDFPNREAADLAL
                     AENRITREIWQSIVNALDTGQSPPQNTPLGGYLGIHGLGRGDQKVHQRYNWTNGCIAL
                     TNAQIDQLSKWLKPGVLVKVW"
     gene            6424..6834
                     /locus_tag="PKZ49_00365"
     CDS             6424..6834
                     /locus_tag="PKZ49_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35040.1"
                     /translation="MDILSMLFGLLIGVIVGIWITHIWLAYQRQESTAKLSRLFDQLW
                     QNHFGLMKEMKLDLDNPEYKFQREFFVLNKNKRFNLKRPCLAYFFDDHEGLSDQLGAL
                     SAYGLIREVNGSSDTPAKYQFNEHFVELLRGKQT"
     gene            complement(6906..7337)
                     /gene="dksA"
                     /locus_tag="PKZ49_00370"
     CDS             complement(6906..7337)
                     /gene="dksA"
                     /locus_tag="PKZ49_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HND35041.1"
                     /translation="MSSEKLKSTTILSNYRPKKGELYMNPDQLAHFRKILEGLRIELG
                     QDIDRTVHSMQEEATVFADPNDRASQESDVTIELRNRDRERKLIKKINETIAKIDSGE
                     YGYCESCGIEIGLNRLEARPTASLCIDCKTLEEIREKQLAK"
     gene            7515..8354
                     /gene="dapF"
                     /locus_tag="PKZ49_00375"
     CDS             7515..8354
                     /gene="dapF"
                     /locus_tag="PKZ49_00375"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HND35042.1"
                     /translation="MKLKFTKMQGLGNDFVVIDAINQKITLTATDIRFLADRHFGIGC
                     DQVLLVETSQNDADFRYRIFNADGNEVEQCGNGARCFVRFVHDHALTVKNEIRVETAS
                     GTIIPKLEESGEVTVNMGIPRFDPADIPFTAEKRALTYSLEINHKQIEISAVSMGNPH
                     AVQIVPNLEQAPVLTEGPLIESHARFPKRVNVGYMQIIDRQSIKLRVFERGAGETLAC
                     GTGACAAVVAGIQRGLLNSNVEVNTQGGKLKISWVGENQPVWMTGPATTVFEGIIVLP
                     VKF"
     gene            8393..10759
                     /locus_tag="PKZ49_00380"
     CDS             8393..10759
                     /locus_tag="PKZ49_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35043.1"
                     /translation="MNIAHKWRFFRSGGFDQVRLETGADIKFLRTLDPKLWAALSCPT
                     NNLEFDSKTLEFIDTDGDGHIRVPEIIAAVEWAASVLKNPEDLTQGADALQLNAIDDS
                     TPEGAALLASAKQILLNIGKKDEATITVEDTADLNKIFASTKFNGDGIIPATAAGDTE
                     TQIVIEDIMKCVGSVQDRSGLPGISAEHIEQFFTEAKAYSEWWQDAERDAANVLPLGE
                     NTEAAKMAFDAVKTKIDDYFTRCKLAEFDQKASDPLNPSLLNYEALTNTNLSSTTEQL
                     SALPIARIEAKKPLPLNEGINPAWANAISSLKKQVIQPLLADKEQLSIDEWQALCDKF
                     AAHQAWLNIKQGAAVEVLGIKRIRSILAGSYQDKVSALLQKDQSLASASDAINSVEKL
                     IRYHRNLFQLLNNFVSFRDFYTAQNKAIFQVGSLYLDGRSCDFCLRVSDINKHSSMAN
                     LSGTYLAYCECQRRGGSEKMYIAAAFTNGDADNLMVGRNGIFYDRKGQDWDATIVRII
                     EHPISVRQAFWYPYKRIGKMIGEQIEKMASAREKSVQDQAASGIADASQKAEAGKPPT
                     PPFDVGKFAGIFAAIGLAIGAIGTAIASVLTGFLSLVWWQMPLAILGLILLISGPSVL
                     IAFLKLRKRNLAPLLDGNGWAVNTRAIINIPFGTSLTHMATLPVGAQRSLVDPYAEKK
                     SPWKTYLFILVLLGSIAYLLHSGHLNQGTIDELRKRFSSNKAEVTQEASGTQEISPTP
                     PQTETLPDNSRIETDNTPQSAPTESKSESAAPSGPASIAPAPKPSVAP"
     gene            10908..12602
                     /locus_tag="PKZ49_00385"
     CDS             10908..12602
                     /locus_tag="PKZ49_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HND35044.1"
                     /translation="MRTLITYIMVFPRRSTFVLVALLFAGVAEALSLTALLPLISIAV
                     GETDDSKIGRFMVQALERIGIAPTIDAILLIIVGGMLIKGVILLLTNRQVGYTVAHVA
                     TALRLDLIEALLASRWQYYLRQPVGSLANSVATEAYRAANGFEHSVNVLALAIQVIVH
                     AIVALFISWQATFGSLLIGIVLFIALHRLVRATRRAGTKQTHLLRHLLSYLSDVLSSV
                     KSLKAMARDNVADAILHEQSIQLEKATRKEVISRAALLALQEPILAALTASGLYIALV
                     VWNLSLPEVMVMVFLLTRILGLLNKTQRRYQQLTVQESAYWALRTAAEDARAAAERAT
                     GTITPTLQQGISLRHAAFDYDRKPIFQDLNIEIPIKSFTAVIGSSGVGKSTLLDLLCG
                     LAEPKSGEILIDGVPLHNIDLRQWRHLIGYVSQDTVLLHDTILNNTLVGAPALTIEDA
                     EWALKQAGAWDFISSFPEGIQTVVGERGGMLSGGQRQRIAIARALAHQPLLLLLDEPT
                     SALDPQSEQLICETLKKLAEDYTIVAVSHQPAVINAADRVFILSNGQADLLTNTPK"
     gene            complement(12604..13833)
                     /locus_tag="PKZ49_00390"
     CDS             complement(12604..13833)
                     /locus_tag="PKZ49_00390"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HND35045.1"
                     /translation="MEVFLVGGSVRDELLGLPVKDHDYVVVGSNPEKMVQNGFRPVGK
                     DFPVFLHPVTHEQYALARTERKVSRGYKGFEVYASPDVTLQEDLARRDLTINAIAKDQ
                     TGKIIDPFNGIADLEAGILRHISPAFVEDPVRILRVARFAARFGFHIAPETMELMKEM
                     VHTGEVDALVPERLWQEIARGLMEKRPSQMFYALRACNALSRVLPEVDVLFGVPQPAQ
                     HHPEIDTGVHIMIAIDYAAQNNYSLAVRFATLTHDLGKGTTPPSEWPRHVGHETRSVT
                     LVKDLCERIRIPKEERDLALIVARYHGDVHRAEELRPATIADILQATDAYRKPDRFAA
                     FLQACACDFHGRPGYAEKPYAQSVRLNKAFDAARSVDAGVIAANLKQKDIDLLDLPSM
                     INRSIREARIAAIVNSQ"
     gene            complement(13849..15762)
                     /locus_tag="PKZ49_00395"
     CDS             complement(13849..15762)
                     /locus_tag="PKZ49_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HND35046.1"
                     /translation="MRKIIFCLMLVVSTIVTAASLEDDFLKLREAFRVGDAAKLALYA
                     PRFQRHALAPYAEYYQIRLYLETTSVDSIQAFLQRHQGSLIGDKLRGDWLRVLGKRQQ
                     WTLFAEEYPKLINKDEGLICYALQLRLASKDKEVIEEARSLWFTEKDMPDSCTPVFSQ
                     LIANQKLSHEDIWFRIRLALEAGNTGVARQVSRYLPGNHELNAQKLNEASKNPLRYLE
                     KFQGSYSNRADREIVLFALGRLLRSEINQAYVHLLKIRNHFPPTDQSFFMSSLAYRAA
                     LKQDDRALDWFIQAVDKHPYPLSEAKHAWMGRVALRKGNWNHVLQIIDNMPDNMQKMD
                     VWRYWKARALLKNGELGEANAILAPLSNEHSYYGQMAKEELGVMMSSPAANYHPSSQE
                     IHAMQQNPGIQRALALYQFNQRIEAAREWIWTIRNFTDEQLIAAAEVAFRHGIYDRAI
                     NTAIQTVSQHNFNLRYLAPYRDQMREVLKHQELDEAFVYGLIRQESRFLADVKSTAGA
                     MGLMQLMPATAKWVAGKLGMQQFQNHLVTDINTNLRLGTYYLKHVLSLFDNQPLLAAA
                     AYNAGPGRAKQWRDPEKPLEGAIYAETIPFNETRDYVKVVLNNSVYYANNFGHQNIPP
                     LRQRLGVVAPKPQ"
     gene            complement(15865..17208)
                     /gene="gdhA"
                     /locus_tag="PKZ49_00400"
     CDS             complement(15865..17208)
                     /gene="gdhA"
                     /locus_tag="PKZ49_00400"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HND35047.1"
                     /translation="MKYKGIEEFRNYVSERNPGQPEYMQAVTEVIESLWPFITNNPRY
                     AEHGLLDRIVEPERIIIFRVAWVDDHGEVKVNRGYRIQHSSAIGPYKGGIRFHPSVNL
                     SILKFLAFEQTFKNALTTLPMGGGKGGSDFDPKGRSPGEVMRFCQAFVSELFRHVGSD
                     TDVPAGDIGVGGREVGFMAGMVKKLTNRADCVFTGKGISFGGSLVRPEATGYGTVYFV
                     EEMLKHVGQSLNGLRVAVSGSGNVAQFAVEKAMAFGAKVITVSDSSGTIVDEAGFTPE
                     KLAILAEVKNHLYGRVSDYAERVGAKFIPSERPWRVPVDVALPCATQNEVSEDDAATL
                     IKNGVICVAEGANMPCTAGAVKRFEHAKVLFAPGKASNAGGVATSGLEMSQQAMRLFW
                     SHEEVDARLQEIMRAIHNSCLRYGEQSDGRINYVDGANIAGFVKVADAMLAQGVI"
     gene            complement(17543..18787)
                     /locus_tag="PKZ49_00405"
     CDS             complement(17543..18787)
                     /locus_tag="PKZ49_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35048.1"
                     /translation="MRLVFKDFFRYCSTIIIVFNLSWIISACQTFDQSSQQSRFIENP
                     AAIGSQFPYLSSLPDGSMLMSWIEGKGNHQVLKFAIYRQDRWVRIGQAVQGSGWYVNW
                     ADYPAVVAIDDSFWVAHWRVKSKTEQIYDYDVFFSISVDAGVTWSAPQKPYQDDTAAA
                     HGFVSVFPALGQAGIVWLDGRDVAKKISDRFSLRYALIHRNGQIDSDRVIDDNTCTCC
                     WTASAVTSKGPVVVWRSRRGDEIRDHHFAQLIDGIWSDPQPVSQEGWSIAGCPVNGPS
                     IATNGKYVAASWFTAEGNRPRVRSAFANDSSLQFNQALDIDDEKPIGRTRVIALTDET
                     AIVIWITAMDKVTRKASVAARSIGMNGNMGQIKRLSEINPGRDSGVPQIVKNRSSVII
                     AWTQTEPPSGVKLLFVPLEIFD"
     gene            complement(18942..19238)
                     /locus_tag="PKZ49_00410"
     CDS             complement(18942..19238)
                     /locus_tag="PKZ49_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HND35049.1"
                     /translation="MNWYRRENNGKLILSLYIQPGTKNTQFAGLHGDALKVSLAAPPI
                     EGKANKALIKFLADCFQVSANQVSIKRGAHSRYKLVEIEQATIDPGLLLEGESS"
     gene            complement(19238..19810)
                     /locus_tag="PKZ49_00415"
     CDS             complement(19238..19810)
                     /locus_tag="PKZ49_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HND35050.1"
                     /translation="MSSQILIFLLETILGLFSLACLLRFYLQLFRVHYDNPLSKFLIA
                     VTNFIVLPTRRVIPSWKGFDLSTFFLAWIGQFLIMVSTSFIQGYELETVSGNTMIGFG
                     LLSLLELVRLTLYIVMAMVIVQAILSWINPYSPIAPLLTSFTRPILGIFQRFIPPIAN
                     VDLSPLFVLVAVQLLLMVLTGLQQEIAVLF"
     gene            complement(19845..20663)
                     /gene="proC"
                     /locus_tag="PKZ49_00420"
     CDS             complement(19845..20663)
                     /gene="proC"
                     /locus_tag="PKZ49_00420"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HND35051.1"
                     /translation="MKITFIGGGNMASALIGGLLQQNFPMTQIHVVEINAETANRINQ
                     QFGVGTTTDLIEGISGSNVIVLAIKPQQLHGVAQALKPLLTSQLVISIAAGVRTRDLS
                     RWLGNYQYIVRAMPNTPALVRAGMTGLYAIPSVSVALRDDATTILGAVGSVLWLEEED
                     QLDAVTAVSGSGPAYLFYFMEAMEQAGVELGLTLDQARELTIHTFIGASQLAYQSHED
                     VAVLRSRVTSKGGTTEQALLSMSQDGVKNEIIRAAFAAYKRAQQLGDELGQVDG"
     gene            complement(20766..20972)
                     /locus_tag="PKZ49_00425"
     CDS             complement(20766..20972)
                     /locus_tag="PKZ49_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HND35052.1"
                     /translation="MGRWLIIIGALLFFLGVVVQFAPWLISWFGKLPGDIRIESERGK
                     IFIPITSMLIISIVITLLINLFKR"
     gene            complement(21046..21864)
                     /gene="mutM"
                     /locus_tag="PKZ49_00430"
     CDS             complement(21046..21864)
                     /gene="mutM"
                     /locus_tag="PKZ49_00430"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HND35053.1"
                     /translation="MPELPEVEITRRGITPALINQNITNVTIRHANLRWPISDRLDQL
                     LVGQRIEAITRRAKYLLLRCTRGFLIIHLGMSGSLRISFDSASVEKHDHFDLKVSSGA
                     VLRYRDPRRFGAILWSDAEKIAQHPLLVNLGVEPLSDTFDGKMLYERSRHRNLSVKVF
                     LMNHQIVVGIGNIYANEALFHAGINPTVLAKRINLKRYEKLADAIKTTLNAAIEAGGS
                     SLRDFVNSEGKPGYFQQHYWVYGRDNQPCKRCGQLITKIKQGQRSSFYCRHCQK"
     gene            complement(21864..22727)
                     /locus_tag="PKZ49_00435"
     CDS             complement(21864..22727)
                     /locus_tag="PKZ49_00435"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HND35054.1"
                     /translation="MASRVWLNLIMFATAVGLAVFLYIKPSFQPQTTYPISSLSKEAV
                     QLIQISSQHGTIELKRSNGDWLMTEPLQARADKTKVERILDLLTTTSDYRLPPENLSR
                     YGLAAPIAQLQVNHELFAFGQIAPVSNHQYVMTQSGIFLISPRHAMLLTVAPTELIDR
                     QLLGEAEIPQQFIFKAMHLQQTEKWLLSSEASDVHLSQDEINQWVHSWQLAQASYITQ
                     NEHEVDETQETLTIVLQSGQPIHFLIMQRDPELVLLRKDQFINYHFLGHSGNQLLSPY
                     SGIRHEERMVE"
     gene            complement(22736..24088)
                     /locus_tag="PKZ49_00440"
     CDS             complement(22736..24088)
                     /locus_tag="PKZ49_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HND35055.1"
                     /translation="MTTITQKLRFNLLVQNGLFVLLLLIIIALAGYAAMQFRYQWDVS
                     QNNRNSLSQASLDVINKMNGPIGITVYATPQDVQLGDIRKIISNFLMIYQRVKPDLVF
                     EFIDPIEQPDLAQEAGIRMNGEIIITFDHRSEHLTAINEQAFTNALMRLVRSEEKRVM
                     VLSGHGERKLDGIANRDMGDFGKKLIETGFKGESLNLASSDIPLETNLLVIASPQTDL
                     LEGEVEKIITYLDQGGSLLWLVDQESLFGLQPLAEKLGITFTPGIVVDPQAKRLKAPV
                     TFALGTIYGQHAITENFDYITVFPFARQIILNENEEWYGVSLVEVAPQGWVETEALGD
                     EVSYDETHDVPGPVSVAVALDRTLDDREQRVVVIGSSHFLANTYLGNGGNIDFGVNII
                     NWLAGDDDLILIQPRATIDSQLVLSETSLTLIAVGFLIVLPLFLLVTGFVIWWQRRAS
                     "
     gene            complement(24109..24852)
                     /locus_tag="PKZ49_00445"
     CDS             complement(24109..24852)
                     /locus_tag="PKZ49_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HND35056.1"
                     /translation="MILTIAAKEIKVLFVSPLAWVFLAAIQLILTWVFLGRLDAFIEL
                     QSQLMLIANPPGATEVIIVPVYAVASIVLLMATPLLTMRLMAEEWRNHTMPLLMAAPV
                     TVTAIVLGKFAGLMIFFLIIIFLVGLLPFSLFLGGTMDLGLILSNTMGLLLLAASFVS
                     LGLYVSSLTTHPAIAAAGTLGLLMMLWIIDIVSTDLGNLLPNLSLLKHYEQFNRGMID
                     SFSVIFFMLFIISFLVLTIRRLDGSRLHG"
     gene            complement(24854..25600)
                     /locus_tag="PKZ49_00450"
     CDS             complement(24854..25600)
                     /locus_tag="PKZ49_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HND35057.1"
                     /translation="MNETACTLIAHDLSRQFGQYAAVQGIEIELKQGEVLGLLGPNGA
                     GKSTTMQMLSGNLAPTRGSIQICGIDLLDHPEEAKQNIGYLPEIPPLYKELTVDEYLR
                     FVAKLHRVESSQILDALVRVKDRCGLNQVGKKLIGILSKGFQQRVAIAQAIIHQPKVI
                     ILDEPSVGLDPNQIREIRELIRELGKTASIILSTHILSEVETICDRVQILNKGGVVYS
                     AALSDLKQKSINLEQIFEQFTLKKELVGDY"
     gene            26033..26734
                     /locus_tag="PKZ49_00455"
     CDS             26033..26734
                     /locus_tag="PKZ49_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HND35058.1"
                     /translation="MKKIISAKLYLIGVSVLPLLMLSNMVAADAHNRQGESETEKTQG
                     YNVNDRGAVSRNSTGLCWRTGFWTEEMAIPECDPDDTPPVEKQAATPAPAPLTSPEKI
                     TLSADALFDFDKAVLKPQGKASLDEFVGKLNMISYDLVIAVGHTDRLGSDEYNKRLSI
                     RRAEAVKGYLVSKGISADRVFTDGKGESDPVTGDTCRGEKRTKALIDCLQPDRRVEIE
                     VAGTRSDRYETIEQK"
     gene            26797..28131
                     /locus_tag="PKZ49_00460"
     CDS             26797..28131
                     /locus_tag="PKZ49_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HND35059.1"
                     /translation="MNVTSTALRIDTLLEARWIIPVDPSHTTLIDHAIAIQDGKIIAI
                     LPTTEALSQFSPKQHFVLNHHLIIPGLINTHTHAAMTLMRGMADDLPLMDWLKNCIWP
                     VEKQWVDTDFVLAGTTLACAEMLKGGITCFNDMYFFPEKAVEAAAQTGMRAAIGLISI
                     EFPTAYATDANDYLAKGLMLREQYLEYPTLSFCLAPHAPYTVSDSTLSNIFTFANQLD
                     LPIHMHLHETSEEIASSLHDFGIRPIERLQKLNLLGPNLIGVHMVHLTNEEINLLKLH
                     NCNVVHCPSSNMKLASGIAPIVDLVNASINVAIGTDGAASNNRLDMFEEMRLTSLLGK
                     VTHGAQALPAHQVLRMATLNGAITLGIEALTGSLSVGKAADITAIDFSGLELSPCFNP
                     LSHLIYVAGREHVSHVWVNGQLLLNEGELVTLNEAEIIERTQYWQNRITSKQ"
     gene            28142..28849
                     /gene="ubiG"
                     /locus_tag="PKZ49_00465"
     CDS             28142..28849
                     /gene="ubiG"
                     /locus_tag="PKZ49_00465"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HND35060.1"
                     /translation="MDNNGINADPLELEKFSQLAHHWWDPNSEFKPLHEINPLRLNYI
                     DQVSGGISGKSVVDVGCGGGILSESMAARGANVTGIDLSDKALKVAKLHLLESGHQVD
                     YRKITVEMLATEHPRHFDVVTCMEMIEHVPDPASVIKSCAQLVKPGGWVFFSTLNRNP
                     KAYLFAIIGAEYILKLLPRGTHEYAKFIKPSELAHMARDAGLTEARIIGMTYNPITKI
                     YALESDTSVNYIMAFQA"
     gene            28888..29535
                     /locus_tag="PKZ49_00470"
     CDS             28888..29535
                     /locus_tag="PKZ49_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HND35061.1"
                     /translation="MVDAILFDFDGTIADTAPDLGHALNRQRINRGLPSLPISIIRKQ
                     ASAGSRGLLYLGFNLVPTHPEYELMREEFLQFYTERLCHDTCLFPGIADILDHLDSSN
                     IPWGIVTNKPSRFTKPIVQSLGLINRAACIISGDDTPHTKPHPEPLLHACNQINAKPA
                     NCIYLGDDIRDVKASLAAGIKPIVARYGYLGNDGPPESWGADHLIDHPKELAHYL"
     gene            29772..30200
                     /locus_tag="PKZ49_00475"
     CDS             29772..30200
                     /locus_tag="PKZ49_00475"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HND35062.1"
                     /translation="MHHVKKWFCYFAIFYLFIDSCVADSTAPEHVQGAQTIDTLTAKL
                     FFDQKIPFIDVRKYEDFIAGHIPGAYHLSIKSDFDELRLRAIVDKESPVVIYCNGIYC
                     MGSSIAAQKAVNWGWKTIYYYREGMPAWKNNEYPIQAQQN"
     gene            30260..30841
                     /locus_tag="PKZ49_00480"
     CDS             30260..30841
                     /locus_tag="PKZ49_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HND35063.1"
                     /translation="MSKKKILMLVGDYVEDYEAMVPFQALQIIGHIVHTVCPDKKSGD
                     TVRTAVHDFEGDQTYSEKPGHLFRLNASFSKIKVEQYDALIIPGGRSPEYIRLNPKVI
                     KIVQHFAQQGKPIAAICHGAQLLVAADSVRGRTCSAYPTLEPDVKRAGGKYIDIPMDK
                     AHQDQNLVTAPGWPAHPDWLAKFLDLLGTKIKP"
     gene            30863..>31484
                     /locus_tag="PKZ49_00485"
     CDS             30863..>31484
                     /locus_tag="PKZ49_00485"
                     /inference="COORDINATES: protein motif:HMM:NF017773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HND35064.1"
                     /translation="MLFITSRLPTINTEPELNKNFQFNLGDNSSGRELFCCKRLKKDL
                     YEEIGSKNLMQAIKESKYRQVLIYIHGFSNLPEAVFKDAEELQLLCNKKKADEVLILP
                     IIWPCDNDLGIVKDYWDDQKSADQSSFAFARMLQKFMEWRNSPEYNLKNNHCLKRINV
                     LAHSMGNRVFRQTLVNWRKYDQPQGLPLLFRNSFLVAADILNESLHK"
BASE COUNT         9220 a         7232 c         6105 g         8927 t
ORIGIN      
        1 ctaataactt atcccttgct gaatcgaccc gccgctgcac attgtcttct gaacagttgg
       61 tttgttgcgc aaccgaggta accgattggc ttacttctag gtgccagtat ttgctttcga
      121 agttagggaa gcgagtaaag ccgaaataag gatttactac cgaaaattca tcggcagata
      181 atcccctttc aatttcttct ttactccaaa catagaattt tccttcctca tgttcagaat
      241 ctgcatctaa ggatgaataa aaaccgccct caccctgttt ataactcgat tgcatttcgc
      301 gaattgccca actcgccgtc tcttcaacaa tttgtttata caatggatca gccgttgcaa
      361 gccatatatc ggtataaagt tgcaataaag ggccattatc gtagagcatc ttttcgaaat
      421 ggggaattcg ccaatattga tcggtactat accggaagaa acctccccct aattggtcgt
      481 aaataccgcc ctttgccatt ttctccagcg tatgtttcac aattctcagt gcccctgcat
      541 cgctatcata aaaatagcaa tgcaggcaaa atagtaattc tgctggatga agaaatttag
      601 gtgcctgacc aaaaccaccg tactcccgat caaaattctc atctaattga gtcaatgctc
      661 ttaagatcaa ttgtcgagaa atgactggaa tcttggcccc tgtggtaggt aatgtatctg
      721 cgagtaattt cagcagcgcc gtattttgtt tctcaatatc atcggttcga gttcgatacg
      781 tttctgcaat cttgggtaac aattccaaaa aaccgggcat accatgacgt gatactttag
      841 gaaaataagt gcctccaaaa aaaggctttt gctctggcgt cagaaacatc gttaaaggcc
      901 atccaccatg gcgctgattg atcatgtgat gcgccgtttg atagatttga tcaatatcag
      961 gtcgttcttc cctatcaact ttgatattga cgaaatattg attcattgcc gcagcgactt
     1021 cagcatcttg aaatgattca ttggccatca catggcacca gtgacacgct gaatagccaa
     1081 tcgaaagtag aattggctta ttcaattttt tagcgagttc tagcgcttct ttattccatg
     1141 gatgccattc aactgggtta tccacatgtt gcaaaagata ggggcttgtt tcatgtatca
     1201 ggtgatttgg catgtcagtc tgtgattgcg ccacggctag cggtcgacac aagttttgcg
     1261 tatttcgcta acacaccccg atgataccgg gccttaggag gttgccaagc tgcacgcctt
     1321 ttttcaagct catcttcaga tatattgagt tgcaataatc gttgcgtcgc atcaatcgta
     1381 atggagtctc cctctctaac taaggcaatt accccgccta caaatgcttc aggggaaacg
     1441 tggccaacaa ccattccata ggttccccct gaaaatcgac cgtctgtaat tagaccaact
     1501 gaatcaccca gcccttctcc aataagtgca gaggttgggg acagcatttc ccgcatacct
     1561 ggcccacctt taggcccttc ataacgaatc actaccacgt cacccgcatg aatttctctt
     1621 gccagaattg cagacataca cgattcctca gaatcgaaaa cccttgccgg ccctgtaatt
     1681 ttaggattct taacaccgga tattttggca accgcacctt ctggtgacaa atttcctttg
     1741 agaattgcaa ggtgaccttg ttcataaagt ggatcacccc attgacgaat aattttttga
     1801 ttttgtggag gctgatccgg aacattatga agtacttgct ctattgtctc accgctgatt
     1861 gtcaagcaat caccatgaag caagccattg tttagaagca tcttcatcac ttgcgggatt
     1921 ccacccgctt tatgaagatc agccgttaca tattgtccag aaggtttaag atcgcatatt
     1981 acgggaactc gtgcacgaat cacttcgaaa tcattgatat caagctcaac ttctgctgca
     2041 tgggcaatag ctagtaaatg caatactgca ttcgttgaac cacccaccgc catcacaaca
     2101 gaaatcgcat tttcaagtga ttttctcgta attaaatcac gaggatgaat cccttttttt
     2161 attgcttgaa cgagcacttc cgcagactgt gcagcactta cctttttctc ttcatcttct
     2221 gcagccatcg ttgaggaata aggcaaactc atccccatcg cttcaattgc agatgacatc
     2281 gtatttgctg taaacatacc accacatgag cctgcacctg ggcaagcatg gcgctcaact
     2341 tctaacaact ctttggcatc aattttatgt gcagtaaact gccctaccgc ttcaaatgca
     2401 ctcacgattg ttaaatcttg acctttataa tgacccggtt ttatcgttcc gccatagacg
     2461 aaaatagcag gaatattcat tcttgcaagc gcaatcaagg ccccgggcat attcttatcg
     2521 cagccaccaa tcgcaataac cccatccata ctttcagctt gcacggctgt ttcgattgaa
     2581 tctgcgatca cttcgcgaga taccagagag tacttcattc cctcggttcc catcgaaata
     2641 ccatcggata cagtaatggt gccaaacatt tgtggcattg cccccgctct ttttagcgct
     2701 cgttctgcac taagagataa gtcatttaat cccatgttgc aaggagtaat cgtagaatat
     2761 ccatttgcca cacctacaat tggcttgtca aaatctccat cactaaatcc aacagccctt
     2821 agcatagctc gatttggagc tcgcttggct ccctgagtga tgttctgact tcgcttattg
     2881 tctggcatga tctctcctaa atgctattaa atatatccta tcaacgatta atctcagtcg
     2941 taactgatat tgaaaactaa ttgccttata atgcttattt taccgtaaat taacaactta
     3001 accatgttaa tccacccaca gtttaaccct gttgcgcttt cgattggccc gttatctatc
     3061 cattggtatg gtttgatgta tctgattggc ttcgcattct ttattttgct gggtcggtat
     3121 cgaataaagc aattcaacca atccgggatt acctttacta ttctcgatga tgccctattt
     3181 tatggtgtat taggcgtcat actcggagga cgagtagggc atgtcttgtt ttaccaattc
     3241 agctattatc ttgcgcaccc tctagaaatt tttgcgattt ggcaaggtgg aatgtcgttt
     3301 catggaggac tcattggcgt tcttcttgca atgttttttt tagctcgaaa atatcaacta
     3361 aaatggctag ctgttactga tttcattgcg ccacttgtcc caattggcct tggtgcgggt
     3421 cgtatcggaa attttatcaa tggagaacta tggggaaggc cgactgaagt tacctgggga
     3481 atgatttttc ctcatgtaga ccatatacca cgtcaccctt cgcagttata tcagtttgcg
     3541 ctggaaggag tcgtgctatt tattttactc tgggtctatt ctaataaacc gcgttcagtt
     3601 ggcgctgttt ctggtctatt cctaattggc tacggattat tccgctcatt ttctgaattt
     3661 tttaggcaac ccgacgatgg ctttatgggg caattaacct taggaatcag catgggacag
     3721 tggttatcgc tcccaatgat tataggcggt atttttcttc ttatacgctc atcacgcctt
     3781 catgttcccg ctcatcgata acctagccat aggaattcaa cgataacact ctgaattaac
     3841 aagtaaaaaa gcaaaaggcc ctggtaaggg ccttttttat caaacaatct aaataattta
     3901 tttatacatt gattttttaa atgcacgatc aattcgagtt tctgcctcaa tagagcgcgc
     3961 attagcttga tttgcaatat cacgtgcctc cgctgcaaca cgattagcat tgtccgctgc
     4021 tgcacgagcc gcagctgcat ctgcttttgc agaactgata tcagatttaa ttgcattgac
     4081 ttcagctttt agctcatcaa attgagcggt tgttgcacat cctgtcaacg ctaccgttgc
     4141 gcctaatgca acgatcgtta atgcattttt tacatttcta ttcatattat tatctcctcc
     4201 aaggtgaata aattagggtt aacaatcata tacaatggga ataataagct tgctaaccct
     4261 atactttaaa taataaaacc acgacaacat agcgatgcta catgattttt ttagcaatat
     4321 aaagcaagaa gcatatcttc agtaataaag tgtgcatccc ctcagcctta acggcatctt
     4381 tgtatgaata tgagtaaaaa ttattaattc catttatgaa aatatcgaaa tacccgatca
     4441 aggaacacac gactactttg agataagtat aggaacacca ttcctttcga aaatagctaa
     4501 ctctaatgcc gattgatcaa tctcaatatt tttgcgtgtg tggttagcgt tttttccagt
     4561 tgtagtcttt tcaataaagc ttgcaatcac atcgcgtact ttctggccat aatcaacata
     4621 ttctgcttca tcttcatcca aaggtgggtg caactctaca aaaagggaat cagctaacca
     4681 accaattttt atcggctgat tcagaagttg tactggggtt cccaccgtta ctttctcaaa
     4741 caacgtttca atatcctctg ggtacattcg taagcaacca tgggttacgc gcataccaat
     4801 cccaaagggt ttatcagtgc catgtatcaa ataaccagca gtccccaaat agatcgcatg
     4861 acgacccagt ggattattag gccccggtaa aacaatgggt ggtggcggtg gatcacctgc
     4921 tgcaattgct tcatctttga ttgattgagg agggatccac gtaggatctt tctgttttct
     4981 aacaactgat gttttgccaa gcggtgttct ccaatccatc cttccaatac tgatggggtg
     5041 ggtaatcacc actggtttct tacctttatt cggcttaggg aaataataga ggcgcatttc
     5101 aggcaagttg ataataaaac ctgtccgatc tccctgcggt atgatatacc gtttaggcaa
     5161 tacaatttca gcaccttcct cagggagcca gcgatcaaca gttggattcg caagcatcat
     5221 ttcatcctga cctataccaa aacgtctcgc aacatccaaa agtgtttcat cgcgattagc
     5281 aggaatcgtt ttaatttgcc caaataaatc tacatctcca gggggtaata tccatgatgc
     5341 ggattgagcg aaggttgaaa agataaaaac tacaacccca aacattactc tactcataat
     5401 cgaattatct aaaaacatat acccaccttt ccttcccatt ttcttttcct gaattaagaa
     5461 caccgagcat tcaagctgca ctaaatttga taacttggct aatttaaatt aagtcaacga
     5521 taagaaatcg atttaaaaat atgcctctaa ccactaccag ttttctacca aaccttcact
     5581 agcacaccag gtttgagcca tttactcaac tgatcaatct gtgcattggt tagcgcaata
     5641 caaccgttag tccaattgta tcgttgatga accttttgat cgccccgtcc gagaccatgg
     5701 atacctaaat aaccgcccag aggagtattc tgcggaggag attgtccagt atcaagtgcg
     5761 ttcactattg actgccagat ttctcttgta atgcggttct ctgctaaagc cagatccgct
     5821 gcttctcgat taggaaagtc aaaaacaaaa aaacgatgga agcgacgcga ctctttaatc
     5881 catccaatcc ggaacgtacc aattggggtc cggttgtccc ccatcattct ggccttgctt
     5941 gctccgtagc gtccaattga aatgttatca aaactcagtt caattttctc accccgcatt
     6001 actgacaaag tcaaattatt ggtatcaaca tcaacccaaa catctccatg tgcatttacc
     6061 aagcagcttg ataatatgcc taaaatcaaa agaatgaact tattgtacat agcttattag
     6121 tagatttatc taatagtaaa tcgaacaaaa aacatcgtaa ttagaccact tagttagaaa
     6181 catcaattca ctgagataaa gtttttatac cgctatttaa tagataatgc agctaaacta
     6241 gtagcatctg ctaatatctg ttagcgtaaa ccacatatat cagagtgcta gtatctacgt
     6301 taatctagca gaattcctgg taacataaat tgcatttatt tataaaatag cttttggcgt
     6361 attatatcca cacgcgcaag gtcatctaat ttttaaaaca ataacagagg gggagaaata
     6421 ataatggata tcctaagcat gctctttggc ttattaattg gcgttatcgt cggtatttgg
     6481 atcactcata tttggctagc ctatcaacgt caggaatcca cagcgaagct gtcgcgactg
     6541 tttgatcaat tatggcaaaa tcacttcggc ctcatgaaag aaatgaaact cgatctagat
     6601 aatccagaat ataaattcca gcgagaattc tttgtcttaa ataaaaacaa gcgttttaac
     6661 cttaaaagac catgtttagc atatttcttc gatgatcatg aagggttaag tgatcaatta
     6721 ggtgccttat cagcttatgg attgatcaga gaagtaaatg gatcaagcga taccccggct
     6781 aaatatcaat ttaacgagca ttttgttgaa ttgcttcgcg gcaaacaaac ataataaaat
     6841 acattgcctg attaaaaaaa gaacaaaact agcctattag cgttattttt gttcttttta
     6901 ttatcttact tagcaagttg cttttctctt atctcctcta gcgttttaca gtcaatacat
     6961 aacgaagctg tagggcgcgc ttctagtcga tttaatccaa tttcaatacc acagctctca
     7021 caataaccat actcgccaga atcaatttta gcgattgtct cattaatctt cttgataagc
     7081 tttctctccc tatcgcgatt acgcaactca atcgtaacat cggattcttg actagcccta
     7141 tcattaggat ctgcaaaaac ggttgcttct tcctgcatcg agtgaacagt acgatctata
     7201 tcttggccta attctatcct gaggccctct aaaatctttc taaaatgggc caattgatca
     7261 ggattcatat aaagctctcc tttctttggc ctataatttg ataggattgt cgttgatttc
     7321 aatttttctg aagacatatt gagctcctat ttctatttcc taattaaccc ctataattag
     7381 caaaatttgc agggataaca gaaaaagcag gttattgcaa gcatcaggtt cgagctttat
     7441 ttaatagatc aggcaataag ccattgaaat tattaggcac acatgcgcta accgctttcc
     7501 atcacaagca agctatgaag ttaaaattca ctaaaatgca gggactagga aatgactttg
     7561 ttgtcattga cgctatcaat cagaaaatta cactcaccgc caccgatatt cgatttttgg
     7621 ctgaccggca ttttggtatt ggttgcgatc aagttctatt agttgaaaca tctcaaaatg
     7681 atgctgactt ccgttatcgc atattcaacg cagatggaaa tgaagttgag caatgtggaa
     7741 atggggcacg ctgctttgtt cgctttgttc acgaccatgc attgacggtt aaaaatgaaa
     7801 ttcgtgttga aaccgcaagt ggtactatca taccgaaact agaagaaagt ggtgaagtca
     7861 ctgtcaacat gggtatacct cgctttgatc ctgctgatat tccatttact gctgaaaaac
     7921 gtgcactaac ttattctctc gagattaatc ataaacagat cgaaatcagc gctgtttcca
     7981 tgggaaaccc gcatgctgtg caaattgtac caaatctaga gcaagcgcca gttttgactg
     8041 aaggtccgct aattgagtca catgcgcgtt tcccaaaacg agtcaatgtc ggttatatgc
     8101 aaattattga tcgccaatcg atcaaattac gtgtcttcga gcgaggggct ggtgaaacac
     8161 ttgcatgtgg gacaggcgct tgtgctgcgg ttgtagcagg aatacagagg ggcttattga
     8221 attctaatgt agaagttaat acccaggggg gtaaattaaa gataagctgg gttggtgaaa
     8281 atcagcctgt atggatgacg gggccagcaa cgaccgtttt cgagggtata atagtccttc
     8341 cggtcaaatt ctaagaaagt taaaaaaaat tctcacatat aaggaaagca taatgaatat
     8401 agctcataaa tggcgctttt tccgatccgg tggctttgat caagttcgtt tagaaacagg
     8461 ggcggatatt aaattcctta gaacacttga tcccaagtta tgggctgcct tgagttgtcc
     8521 aacgaacaat ctagaatttg acagtaaaac gcttgaattc attgacactg atggtgatgg
     8581 acatatccgt gttccagaaa ttattgctgc agtcgaatgg gcagcttctg ttcttaaaaa
     8641 tcctgaagat ctcacacaag gagcggatgc cctacaactt aacgcgattg acgatagcac
     8701 tcctgaaggc gctgcactac tggcatcggc taaacaaatt ttactcaata ttggtaaaaa
     8761 agacgaagca acaattactg tcgaagatac tgcagatctc aataaaattt ttgccagcac
     8821 taaattcaat ggcgacggta ttattcctgc cacggctgca ggtgacactg aaactcaaat
     8881 tgtcattgaa gatatcatga agtgtgtcgg atctgtgcaa gatcgctctg gactcccagg
     8941 tatttcagca gaacacattg agcaattctt tacagaagcc aaggcatact cagaatggtg
     9001 gcaagatgcg gaacgagatg ctgccaacgt attaccttta ggggaaaata ccgaagctgc
     9061 aaaaatggct tttgatgcgg tcaaaacaaa aatagatgac tattttacgc gttgtaagct
     9121 tgctgaattt gatcaaaaag caagcgatcc attgaatcct tctttattaa attatgaagc
     9181 gcttacaaat accaatctct ccagcacaac agagcagctc tctgcattac cgattgccag
     9241 aatcgaagca aaaaaaccgc ttccacttaa cgaaggtatc aatccggctt gggctaatgc
     9301 cattagctca ctaaaaaaac aagttattca gcccttatta gcagataagg agcaactgag
     9361 catagatgaa tggcaagcgc tttgtgacaa atttgctgcg caccaagcct ggttaaatat
     9421 caagcagggc gctgccgtag aagttcttgg gattaaacga atccgttcaa ttctggctgg
     9481 aagctaccag gataaagtca gtgcattgct gcaaaaagac caatcccttg cgagcgcatc
     9541 tgatgcaatt aattcagttg aaaaactgat tcgctatcat cgcaatcttt ttcaactttt
     9601 aaataatttt gtttcgtttc gtgattttta cacagcgcaa aataaggcaa tttttcaggt
     9661 aggctcgctt tatttagacg gtcgtagctg tgatttttgt ctccgtgtta gcgacatcaa
     9721 caagcatagc agtatggcta atttaagcgg tacttatcta gcctactgtg aatgtcaacg
     9781 tagaggtggt agcgagaaga tgtatattgc agcagccttc actaatggtg atgccgataa
     9841 tttaatggtt ggccgtaatg gtatttttta cgatagaaaa ggacaggact gggatgcaac
     9901 catcgtcagg atcattgaac atcccattag tgttcgtcaa gccttctggt acccttacaa
     9961 acgcatcgga aaaatgattg gtgagcaaat cgagaaaatg gctagcgcac gggagaaatc
    10021 ggttcaagac caggcggctt ccggcatagc agatgcttca caaaaggcag aagctggcaa
    10081 acctccaaca cccccctttg acgttggaaa atttgcaggg atctttgctg caattggtct
    10141 tgctatcggt gcgattggga ccgcaattgc atcggttctg actggcttcc tcagtctcgt
    10201 atggtggcaa atgccgctcg cgattcttgg tttgatcctc ttaatatctg gtccttccgt
    10261 attaatcgct tttctgaagc ttagaaaacg caacctagca ccattactcg atggaaatgg
    10321 ttgggctgtt aatacacgag caattattaa tattccgttt ggcacttcat tgactcacat
    10381 ggcaacatta ccagttgggg cgcaacgttc cttggtcgat ccctatgcag agaaaaaaag
    10441 cccgtggaag acttatctat tcattctggt cttattagga agtattgctt atcttttaca
    10501 tagtggacac cttaatcaag gtacgattga tgagctgaga aaacgtttct catccaataa
    10561 agcagaagta acccaggaag catcaggtac tcaggaaatt tcaccaacac caccccaaac
    10621 agaaacatta cctgataatt caagaattga gactgacaat accccccagt ctgctccaac
    10681 agaatcaaaa agtgaatcag cagcgccttc tggaccagcc tcaattgcgc cagcaccgaa
    10741 gcctagtgtt gccccctgag tgctgctacc atcattttat gtataagcgg gcataatcat
    10801 tactatattt tgaccgactg tgtccataat tcttagactc tttttacgat tttaaaacca
    10861 ataaccctac agcgagctag cgtctaagaa ataatttcta atcgaatatg cgcacactca
    10921 tcacttatat catggttttc ccgcggcgga gtacattcgt gctcgtcgca ttgttgtttg
    10981 ctggcgttgc ggaagcgttg agtttaacag cgctactgcc attgatatcg attgccgttg
    11041 gcgaaacgga tgattcaaaa attggacgct tcatggttca agcgctagag cggattggta
    11101 ttgctccaac aatcgatgcg atactattaa tcatcgtagg cggcatgctt atcaaaggcg
    11161 ttattctatt gctcaccaat cgccaagtcg gttataccgt tgcccacgtc gcaactgccc
    11221 ttcggttaga tttgatcgag gccttattag cgagccgctg gcaatattac ctccgtcaac
    11281 ccgtcgggtc acttgcaaat tcagtggcta cagaagccta tcgtgcagca aatggtttcg
    11341 aacatagcgt caatgtgctc gcactcgcta tccaggtaat cgttcacgcg attgtggcgc
    11401 tcttcatttc atggcaagca acatttggat cattattaat tgggatcgta ctgttcatcg
    11461 cactccatcg gctggttcgc gcaacaaggc gagcaggaac caagcaaaca catctgctac
    11521 gtcatttatt aagctacctt tccgatgtac ttagttctgt aaaatcacta aaagccatgg
    11581 cacgcgataa tgtagcggat gccattttac atgagcagtc aatacaactc gaaaaagcaa
    11641 cccgcaaaga ggtaatcagt cgagcagcct tactagcatt acaagaaccc attttagcgg
    11701 cattaactgc gagcggactc tacatcgcac tggttgtttg gaacctttca ttgcccgaag
    11761 tcatggtcat ggtatttttg ttaacacgca tactgggctt acttaacaaa acacaacgac
    11821 gttaccagca gctcacagtt caagaaagcg catactgggc acttaggact gcggcagaag
    11881 atgcgcgtgc agctgcagaa agagctaccg gcacgatcac accgacctta caacaaggta
    11941 tcagcctacg acatgctgca tttgattatg atcgtaagcc aatttttcaa gatcttaata
    12001 tcgaaattcc gattaagtca tttactgcgg tcattggttc ttctggggtc ggtaaaagta
    12061 cgctactcga tttgctctgt gggcttgcag aacccaaatc aggagaaata ctcattgatg
    12121 gcgtgccttt acataatatc gatttacgcc agtggcgcca tttaatcggc tatgtatcgc
    12181 aggatacggt tctgctccat gacacaatat taaacaacac gctcgttgga gcacctgcac
    12241 ttacaatcga ggacgcagaa tgggcattaa agcaagcagg agcttgggat tttatcagta
    12301 gtttccccga gggcattcaa accgttgtcg gtgagcgcgg tggtatgctc tcaggggggc
    12361 aacgccaacg aatcgcgatt gcacgcgccc tggctcatca accattgcta ttgctactag
    12421 acgaacctac tagcgcactt gatcctcaaa gtgagcaact catctgtgaa acactcaaaa
    12481 aattagcgga agattatacg attgttgcgg tatcacatca gcctgcagtc atcaatgctg
    12541 cagaccgcgt gtttattctc tccaatggcc aagctgatct actcaccaac acgcccaaat
    12601 gatctactgg ctattcacga ttgcagcaat ccgtgcttct cgaatcgagc gattaatcat
    12661 tgacggcaaa tcaagcaaat cgatatcttt ttgcttcaga ttcgctgcaa taacacccgc
    12721 atccacgctt cttgccgcat caaaagcttt attcagtcga actgattgcg cataaggttt
    12781 ttcagcataa cccggtctcc catgaaaatc acaagcacac gcttgcagga atgctgcaaa
    12841 gcgatctggc tttcgatagg catcggtcgc ttgtaaaata tcagcaatcg ttgcaggtct
    12901 taattcttcc gctcgatgca catctccatg atagcgtgca acgataagcg ctaaatcacg
    12961 ttcttctttc gggatacgta tgcgctcaca taaatctttt acaagtgtca cacttcgtgt
    13021 ttcatgtcct acatgccttg gccattcgct gggtggtgta gtacccttac caagatcatg
    13081 cgttaaggta gcaaatcgaa ccgccaatga ataattattt tgtgctgcat aatcaatggc
    13141 gatcatgata tgcacgcccg tatcgatttc aggatgatgc tgagcgggtt gtggtacacc
    13201 aaataacaca tcgacttcag gcagaacccg cgataaggca ttgcaggctc tcaatgcata
    13261 aaacatctgc gaaggtcgtt tttccatcag cccccgcgca atttcctgcc atagacgctc
    13321 aggaacaagt gcatcgacct cacccgtgtg taccatttcc ttcatcagct ccatcgtttc
    13381 aggtgcgata tgaaaaccga agcgtgctgc aaagcgagcc acgcgcaaaa ttcttaccgg
    13441 atcttcaacg aaagcaggac taatatgcct taagatccct gcttcaagat cagcaatccc
    13501 attgaatgga tcgattattt ttccagtctg atctttggct atcgcattaa tggttaaatc
    13561 tcgtcgcgcc aagtcttcct gtaaagtaac atcaggtgaa gcataaactt cgaatccttt
    13621 gtaccctcgg gatactttac gttcagttct tgctaaagca tattgctcat gcgtcacggg
    13681 atggagaaaa actgggaaat cttttccaac gggccggaag ccgttttgta ccattttctc
    13741 tggatttgaa cccacgacta catagtcgtg atctttcacc ggtaacccaa gcaattcgtc
    13801 tctaactgag ccaccaacga gaaaaacttc catagattct actgaagttt attgaggctt
    13861 cggtgcgacc acacctaatc gttgtctgag tgggggaata ttttggtgcc caaaattatt
    13921 tgcgtagtag acggaattat ttaaaaccac cttcacatag tcgcgtgttt cattaaatgg
    13981 aatggtttct gcatagatgg ccccttctaa gggtttttca ggatcgcgcc actgttttgc
    14041 acggccaggg ccagcattat aagcggcagc cgctagcaaa ggttggttat caaataggga
    14101 caatacgtgt ttcaaataat acgttcctaa gcgcagattg gtattaatat ccgtcacgag
    14161 atggttctga aactgttgca ttcccaattt tccagcaacc cactttgcag tagccggcat
    14221 tagttgcatc agccccattg ctcccgctgt cgatttcaca tcagcgagaa agcgactctc
    14281 ttggcgaatt aacccgtaca caaacgcctc atctaattct tggtgtttta atacctctct
    14341 catctgatca cgataaggtg caagatagcg caagttaaaa ttgtgctgtg agacggtttg
    14401 tatggctgta ttaattgcac ggtcataaat cccatggcga aatgccactt cagctgctgc
    14461 aatcaactgc tcatctgtaa aattgcgaat cgtccagatc cattcacggg ctgcctctat
    14521 ccgctggtta aattgatata aagccagtgc acgctgtatt cccgggtttt gttgcatggc
    14581 atgtatttct tgcgagctag gatgataatt agcagcgggg gaactcatca tgacccctaa
    14641 ctcctctttt gccatttgac catagtagct atgttcattg cttagcggtg ccaaaatcgc
    14701 atttgcttca cctaattccc cgtttttaag taatgctctt gctttccagt atcgccacac
    14761 atccattttt tgcatgttat cgggcatatt atcgataatt tgcaaaacgt gattccagtt
    14821 gcccttacga agcgcaacac gccccatcca cgcatgtttg gcttccgaca gtggataggg
    14881 atgcttgtct acagcttgaa taaaccaatc taatgcacga tcatcttgtt taagcgccgc
    14941 acgataggcc aggctactca taaaaaaaga ttgatccgtt ggtggaaaat ggttacggat
    15001 tttaagcaaa tgtacataag cttgatttat ctcactacgc agcaatcgac ctaacgcaaa
    15061 caaaacaatt tcccgatctg cacgattaga atagcttcct tgaaatttct ccaaatagcg
    15121 gagcggattc tttgaagctt cattcaattt ttgtgcattc aattcatgat taccaggtaa
    15181 gtagcgactc acttgtcttg caacgcctgt attgccagct tctagcgcca agcgaattcg
    15241 aaaccaaata tcttcatgcg ataacttctg attggcaatg agttgactaa aaacaggcgt
    15301 acagctatcc ggcatatcct tttctgtaaa ccaaagtgag cgagcctcct caatcacctc
    15361 tttgtcttta ctagctaagc ggagctgcag tgcgtaacaa atcaatccct catctttatt
    15421 gatgagtttt ggatattctt cagcaaacaa agtccattgc tggcgcttac ctaaaaccct
    15481 taaccaatca cctcttagtt tatcgccaat caagctacct tgatgacgct gaaggaatgc
    15541 ctgaattgaa tcaacgctcg ttgtttcaag atacaaacgg atttgatagt actcggcata
    15601 gggtgccagt gcatggcgct gaaagcgtgg ggcgtataat gcaagcttgg ctgcatcacc
    15661 cactcgaaaa gcttcacgta gcttcagaaa atcatcttct aagctggcag ccgttacaat
    15721 cgtactgacc accagcatta aacaaaatat tatttttctc atgactcagc aatagtcagt
    15781 aaatcccgaa tacaaagctc agacttactg tcagaggctc aaatcccaaa atacctgaaa
    15841 aattaaatct ctatttcaat aaaattatat taccccttgc gcaagcattg catccgctac
    15901 ttttacaaaa cctgctatgt ttgcaccatc gacatagtta attcttccat cagattgctc
    15961 accatagcgc aagcaggaat tatgaatcgc tctcatgatt tcttgtaagc gtgcatcaac
    16021 ttcttcatgt gaccagaaaa gtctcatcgc ttgctgactc atttctaacc cagatgtcgc
    16081 aacccctcct gcattacttg ctttaccggg tgcaaacaat acttttgcat gttcaaaacg
    16141 ttttacagca cctgccgtac aaggcatatt cgcaccttca gctacacaaa tcacgccatt
    16201 cttgatcagc gtcgccgcat catcttcact gacttcattc tgagttgcac aaggcaaagc
    16261 aacatcaacg ggaacccgcc aaggtcgttc gctcggaata aattttgcgc cgacacgctc
    16321 agcatagtcg cttacacgac cataaagatg attttttact tcggccaaaa tcgccaattt
    16381 ctcgggcgtg aagccagctt catcgacgat ggtgccactc gaatccgaaa cggtaatcac
    16441 tttcgcgcca aatgccatcg ctttctcaac tgcaaattgc gcgacattac cagatcctga
    16501 aacagcaaca cgcagcccat tcaacgattg accgacatgt ttcagcattt cttcaacaaa
    16561 ataaactgta ccataacccg ttgcttcggg tcgtaccagg gaaccaccga agctaattcc
    16621 cttccctgta aatacacaat ctgcacgatt cgttaatttc ttaaccatgc ctgccataaa
    16681 gcctacctca cgacccccaa ccccgatatc acctgctggc acatcggtat cagaacctac
    16741 atgacgaaat agctcgctga cgaatgcttg gcaaaaacgc atcacctcac ccgggcttct
    16801 ccctttagga tcaaaatcag accccccttt cccgccaccc ataggtaatg tcgttaaagc
    16861 attcttaaaa gtttgctcaa aagccaaaaa tttaaggatt gaaagattaa cagaaggatg
    16921 gaaacgaatc cctcctttat aagggccaat tgctgaacta tgctgaatac gataaccccg
    16981 attgaccttt acttcaccat gatcatccac ccaggcaact cgaaagataa tgattcgttc
    17041 aggttcgaca attctgtcta ataagccatg ctcagcatac cgagggttat tggtaataaa
    17101 aggccacaaa ctctctatta cttctgtaac agcctgcata tattcaggtt ggcctggatt
    17161 acgttcagaa acataatttc tgaactcttc tattccttta tatttcattc tattttctcc
    17221 tctctgcttg atcagcgttc gttattcccc aaaactttct gccattaatc caattttttt
    17281 ctctgatttt aatgaattct ttttagtcac aaatacaaag tgtaactgga ttacttgcaa
    17341 tattacagcc atcttttatt tgcgtgatga aatcttgatt gattctattt atatctagat
    17401 tttggtcggt taggcttatt caacaattct ttgtagcaat gccttaaatt acaatctcaa
    17461 aattaagaaa cctgaatttt acctttccat agcattgcaa agctggtcag cattcattga
    17521 aataacctaa tcaacgggtc tgctaatcaa atatttccaa aggaacaaaa agtaatttta
    17581 ccccggaagg aggctcagtt tgagtccatg caatgataac tgaggatcga ttcttaacta
    17641 tttgaggcac tccgctatca cgacctggat tgatttcact taagcgtttg atctgtccca
    17701 tatttccatt cataccaatg cttcgagctg ccacgctcgc cttccgagtt accttatcca
    17761 ttgccgttat ccaaatcaca atggctgtct catcagtaag cgctataacg cgtgttctgc
    17821 cgatcggttt ttcatcatca atatccaacg cttgattgaa ttgcaagctg gaatcattgg
    17881 caaaagcact gcgtacacgc ggacgatttc cttcagcggt aaaccaactt gcagccacgt
    17941 attttccgtt tgtagcgata gaggggccat taactgggca tcctgcaata gaccacccct
    18001 cctggctgac gggttgcgga tctgaccata tgccatcgat caactgcgca aaatgatgat
    18061 ctcgaatttc atcgccacgc cgacttcgcc aaactacaac gggccctttc gaagtcactg
    18121 ccgaagcagt ccagcagcat gtgcacgtat tgtcatcaat cactcgatct gagtcaattt
    18181 gcccattccg atgaattaag gcatagcgca acgaaaagcg atcagagatt ttttttgcaa
    18241 cgtctctccc atccaaccag actataccag cctgacctag cgcaggaaaa acagaaacga
    18301 atccatgggc tgcagccgta tcatcttgat atggcttctg cggcgcactc catgttacac
    18361 cggcatcgac agaaatcgag aaaaacacat cgtagtcata aatctgctca gttttagatt
    18421 taacgcgcca gtgagctacc cagaatgaat catcgattgc aacaaccgcg ggataatcag
    18481 cccaattcac ataccacccc gagccttgta cagcttgacc gatacgcacc caacgatcct
    18541 ggcgatagat agcaaatttt aaaacctggt gatttccttt tccttcgatc caactcatga
    18601 gcattgaacc atccggcaag gaagaaagat acgggaattg agatcctatt gctgcaggat
    18661 tctcaatgaa tcgagactgt tgagatgatt gatcaaaagt ttggcacgcg ctaatgatcc
    18721 atgacaaatt gaaaacaatg atgattgtcg agcaatagcg aaaaaagtct ttaaagacaa
    18781 gtcgcatagc ataactcttt gctgtattaa tgaagaagaa ttaccaacct ggttcaacca
    18841 attttagtaa agatgatgaa tccggaaaat gtggcaaatt gtcgcacgat tcatatgacc
    18901 ttccaacaaa aacaggttct cactaccgca ttacgttagt actagctaga ttccccctcg
    18961 agcaataaac caggatcaat cgtagcttgt tcgatttcga caagtttata gcgagaatgc
    19021 gctcctcgtt ttatgcttac ttgattagca gaaacttgaa aacaatccgc taaaaactta
    19081 atgagcgctt tattcgcctt accttctata ggcggtgctg caagactgac cttaagggca
    19141 tcgccatgca agcctgcaaa ttgcgtattt tttgtgcctg gctggatgta aagagataaa
    19201 attagtttgc cattattttc tcggcggtac caattcatta aaacaagacc gctatttcct
    19261 gctgcaatcc agtcaaaacc attagcaaca attgcactgc caccaataca aacaacgggg
    19321 ataaatcgac attggcaatc ggcggaataa atcgttggaa aatgcctaaa attggacgtg
    19381 taaagctcgt aagcaaaggt gcaattgggc tataagggtt aatccaagaa agaattgctt
    19441 gcacgataac catggccatc acgatgtaaa gtgtcaggcg aacaagctct aacagcgata
    19501 acaagccaaa ccctatcatc gtattgcctg ataccgtctc taattcatag ccttgaataa
    19561 aactagtact gaccatgata agaaactgtc ctatccacgc taaaaaaaat gtggatagat
    19621 caaatccctt ccaacttgga atgactcgac gggtaggtag aacgataaaa ttcgtcacag
    19681 caatcaaaaa ttttgatagc gggttatcgt agtgaactcg aaataattgc aaataaaatc
    19741 ttagtaagca cgccagagaa aaaagaccaa gtatcgtctc caacaggaat atcaatattt
    19801 gactagacat tgttcaatct ccaaagaggt tcatactcaa aatttcaacc atctacctga
    19861 cctagttcat caccgagctg ctgagcgcgt ttataggcag caaacgctgc tcgaataatt
    19921 tcattcttaa caccatcttg gctcatactg agcaatgctt gctccgtcgt ccccccttta
    19981 gaagtgacgc gtgatcgtaa caccgcaaca tcctcatgac tttgatatgc aagctggctg
    20041 gcgccaataa atgtgtgaat cgtcaattcc cgagcttggt caagcgttaa acctaattcg
    20101 acgcccgctt gctccattgc ttccataaaa tagaacagat aagctgggcc acttccagaa
    20161 actgcagtta ctgcatccaa ttgatcttcc tcttctaacc acaaaacaga gcctaccgcg
    20221 cccaaaattg tagttgcatc atccctcaac gcgactgaaa cgctgggtat tgcatataaa
    20281 cccgtcattc cagctcttac taaagcgggt gtattcggca tggctctcac aatatactga
    20341 taattgccca accaccgtga caaatcccga gttcgaacac ctgccgcaat cgaaataacc
    20401 aactggctgg ttaacaacgg ttttagcgcc tgagcaaccc catgtagctg ttgtggctta
    20461 attgccaata cgatcacatt gctgcctgaa ataccttcaa tcaagtctgt tgttgtacca
    20521 acgccaaatt gctgattaat tctgttagct gtctcggcat tgatttcgac gacatggatt
    20581 tgtgtcattg gaaagttttg ctggagtaat ccgcctatca atgcacttgc catattgcca
    20641 ccaccaataa aggtaatttt catgtttcac ctatatgttt atatcgttta ttcatggctt
    20701 gggtagtcta tttcataatt aaataaagat taagtcgatt aaagaagaaa attgcctcaa
    20761 ttcatctagc gtttgaataa atttataagc aaggtaatta cgatgctgat aattaacatg
    20821 gaagtaattg gaataaaaat ttttcctctt tcggattcaa ttctgatatc ccctggcaat
    20881 tttccaaacc aactgattaa ccaaggtgca aattgaacaa ccacacccag aaaaaataat
    20941 aatgctccga taataattaa ccaacgaccc atcacaagca tcccacacgg ctcaattact
    21001 tacctctttg attgataagc gattcaattt ataaatttga tttgtttact tctggcaatg
    21061 acgacagtaa aaactagacc gctgtccctg cttgatttta gtaattaatt gcccacagcg
    21121 cttacacggt tgattgtctc ggccataaac ccagtaatgc tgctgaaaat aacctggctt
    21181 cccttcacta ttcacaaaat cacgtaggct actgccaccg gcttcgatag cagcattgag
    21241 tgtcgtttta atcgcgtctg ctaatttctc ataacgtttt agattaatac gttttgctaa
    21301 aacagtcgga tttatccccg catgaaataa tgcttcattt gcgtagatat tcccaatacc
    21361 aaccacgatc tgatgattca tcagaaatac tttaacactg agattccgat gacgagaacg
    21421 ttcatatagc atcttgccat caaaagtatc actcaacggt tctactccga gattaaccaa
    21481 tagtggatgc tgtgctattt tctctgcatc gctccataag attgccccaa aacggcgagg
    21541 atctcggtaa cgcaacacag cgccactact cactttaaga tcaaaatgat catgtttctc
    21601 aacactagct gaatcaaacg aaatgcgtaa gctacccgac attcccagat gaataatcaa
    21661 gaatccacgc gtacatctga gtaaaaggta cttggcgcga cgcgttatcg cttcaattct
    21721 ttgtccaact aaaagttgat caagtctatc tgaaataggc cagcgtaaat ttgcatgtct
    21781 tatcgttaca tttgttatat tttgattgat aagcgctgga gttatgcctc gtcgagtaat
    21841 ttctacctct ggtaactcag gcattactca accattcttt cttcatgcct aatcccagaa
    21901 taaggggata gtagctggtt gcctgagtga cccaagaagt gataattaat aaactggtcc
    21961 ttgcgcaaca ataccaattc aggatcgcgc tgcatgatca aaaaatggat cggttgccct
    22021 gattgcaata cgattgttaa cgtttcttga gtctcgtcga cttcatgttc attttgagtt
    22081 atataagagg cctgagccaa ctgccaagaa tgaacccact ggttaatttc atcctgactc
    22141 agatggacat cacttgcctc agaagatagg agccattttt ccgtttgctg caaatgcatt
    22201 gctttaaaaa taaattgttg tggaatttcc gcttcaccta ataactgacg atcaattagc
    22261 tctgttggtg ctaccgttag taacattgcg tggcgtggcg aaatcaaaaa aatacccgac
    22321 tgcgtcatca catattgatg attagacact ggcgctattt gaccaaaagc aaataattcg
    22381 tgattcactt gtaattgagc gataggtgcc gccaaaccat agcgactcaa attttccggt
    22441 ggtagtcgat aatcacttgt agttgttaat aaatctaaga tacgctccac ttttgtttta
    22501 tcagctctag cttgaagggg ctcagtcatg agccaatcac cattcgaccg ctttaattca
    22561 atggttccat gttgtgaact tatttgaata agctgaacag cttctttcga tagcgacgaa
    22621 attgggtaag ttgtttgcgg ttggaacgat ggcttaatat agagaaaaac tgcaagtcct
    22681 acagcagttg caaacataat caaattaagc caaacacgtg atgccatggc aaatactaac
    22741 tagctctccg ctgccaccaa atcacaaaac cagtaacgag caaaaataaa ggaagcacaa
    22801 tcagaaatcc gactgcgatc aatgtcaagc tcgtctcact caaaaccagt tgactatcaa
    22861 tcgtggcgcg cggttgaatc agaatcaaat catcatctcc agctaaccag ttgatgatat
    22921 taacgccaaa atcaatattg cccccatttc ctagataagt atttgcaaga aaatggctac
    22981 tcccaattac cacaactcgt tgctcccgat catccagcgt gcgatcaagc gccaccgcaa
    23041 cgctaacagg accaggcaca tcatgtgttt catcgtagct aacttcgtct cctaatgctt
    23101 ctgtctcaac ccagccttgc ggggcaactt caactaatga aacaccatac cactcctcgt
    23161 tttcattcag aataatctgt ctcgcaaaag gaaatactgt gatgtaatca aaattctccg
    23221 taatcgcatg ctgtccataa atcgttccca atgcaaatgt aacaggcgct tttaatcgct
    23281 ttgcttgcgg atcaactacg atacctggcg taaaagtaat ccctaatttt tcagcaaggg
    23341 gttgtaaacc aaataatgat tcttgatcga ctaaccacaa taaacttccc ccctgatcca
    23401 ggtaagtgat aattttttct acctctcctt ccagcaaatc agtctgaggt gatgcaatca
    23461 ctaataaatt agtctcaagc ggaatatctg aactcgccag attgagtgat tctcctttga
    23521 atcccgtttc tattaatttt tttccgaaat cgcccatatc gcgattagcg ataccgtcta
    23581 gtttacgttc gccatgtcct gatagaacca tgacccgttt ttcctccgag cgcaccaaac
    23641 gcattaaggc atttgtgaaa gcctgctcat tgatagcggt taaatgttca ctgcgatgat
    23701 cgaatgtaat gataatctca ccattcatcc tgattcctgc ttcttgagct agatcaggct
    23761 gttcaatggg atcgataaat tcaaacacca gatccggctt tactcgctga taaatcatta
    23821 aaaaattgct aatgattttt ctaatatcac ccaattgcac atcttgaggc gttgcataca
    23881 ccgtgatccc aataggacca ttcattttat ttatgacatc aagactcgct tgactaagac
    23941 tatttcgatt gttttgacta acatcccatt gatagcgaaa ttgcattgct gcatatccag
    24001 ctagcgctat tattatcaaa agcaatagga caaatagccc attctgcacg agcagattaa
    24061 agcgtagttt ctgggtaatc gttgtcattc tttggttata actgttgatt atccatgcag
    24121 tcgactccca tccaagcggc ggatagtcag taccaagaaa ctaataatga atagcataaa
    24181 gaaaataaca ctaaatgaat caatcatgcc tcgattgaat tgctcataat gcttgagcaa
    24241 tgatagattc ggcagaagat ttcctaaatc ggttgagaca atatcaatta tccatagcat
    24301 catcagtaaa ccaagcgttc ctgctgcagc aatggctgga tgagttgtta agctggaaac
    24361 gtacagcccc agggaaacaa aacttgctgc taagagtaat aaacccatcg tattactcaa
    24421 tatcaaaccc aaatccatcg tcccacctaa aaaaagtgag aagggaagca atccaacaag
    24481 aaaaataatg atcaggaaaa agatcattaa cccagcaaat ttacccagaa caatcgctgt
    24541 cactgtaaca ggcgccgcca tcaacaaagg cattgtgtga tttcgccatt cttcagccat
    24601 caaacgcatc gttagcaaag gtgttgccat caataacacg atggatgcta ctgcgtaaac
    24661 aggaacgatt atcacttcgg tcgctccggg cgggttggca atcagcatga gctgcgattg
    24721 tagctctata aaagcatcca agcgccctag aaatacccat gtaagtatga gctgaatcgc
    24781 agcaagaaaa acccacgcga gaggggaaac aaataatact tttatctcct tggcagctat
    24841 cgttaaaatc actttaataa tctccaacca gctctttttt tagcgtgaat tgctcaaaaa
    24901 tctgctcgag attgatgctc ttttgtttaa gatctgaaag cgcagcacta taaacaacgc
    24961 cccctttatt taaaatttgt acacgatcac agattgtttc aacttctgac aagatgtgcg
    25021 tagaaagaat aatacttgct gttttaccca gctctcggat tagctcacga atttctctta
    25081 tttggttagg atccagaccc accgaaggtt catccaaaat aatcactttc ggttgatgaa
    25141 tgatcgcctg ggcaatagca actcgctgct gaaaaccttt tgataaaatc ccaataagtt
    25201 ttttccctac ttgattgagt ccacagcgat ctttaacacg caccaatgca tcaagtatct
    25261 gtgacgattc aacacggtga agtttagcta caaaccgtag atattcatcg acagtaagct
    25321 ctttatagag tggtggtatt tctggtaagt acccaatatt ttgtttggct tcttctggat
    25381 gatcaagtag atcaatgcca caaatctgga tcgaacccct ggttggcgca agatttcctg
    25441 aaagcatctg catcgttgta ctttttccag caccattcgg accaagcagc ccgagcactt
    25501 caccttgctt caattctatt tcaatacctt gcacagcagc atattgaccg aattgtcggc
    25561 ttaagtcatg agcaatgagt gtacaagcgg tttcgttcac aggattagat actcttcagt
    25621 aatcaagcca tcaacattca gtgttggata aagcgcaaca acacctgaac tttaaatcgt
    25681 aagaaagtca tcgtcgttaa gttaaaacta ttcgcagcag atcatcagga ttagctagaa
    25741 aagctcttgc tgttttcgca ttaacctaat ttttagatac cggataattg tcggagatat
    25801 tagttgtgta ttcaatcact ctgtcttaat cgcaagacag agtgttgtat atcaacaaca
    25861 acctggtttg acaaaaagca aaacatgtac gcataatccg gcccaagtag cgtgacgaat
    25921 cggttcagag atatgtatca tgggttcatg ttcatgtcgg tgttcagtgg tttcgttaaa
    25981 ccaattggtt ttgaagcaaa attacagaat tttttcgaaa aaggacgata gcatgaaaaa
    26041 gattatatca gccaaattat atctaatagg agtatctgtt ttacccctac tgatgctttc
    26101 aaatatggta gcagccgatg cacacaatag acaaggtgaa agcgaaaccg aaaaaacaca
    26161 aggctataac gtcaatgatc gtggtgccgt atctcggaat tccacaggtt tatgctggcg
    26221 cactggtttc tggacagaag aaatggcaat tccagaatgc gatcctgatg atacaccacc
    26281 agtagaaaag caagcagcta cgcctgctcc ggcaccttta acatctcctg aaaaaattac
    26341 actttcagca gacgcattat tcgattttga taaggcggtt cttaaaccac aaggcaaagc
    26401 atcgctagat gaatttgtag gcaagttgaa catgattagc tacgatctcg tgatcgcagt
    26461 tggacatacc gatcggctcg gatccgatga atacaacaaa cgtttatcaa taagacgtgc
    26521 tgaagctgtt aaaggctatc tcgtatctaa aggtatcagt gcagatcgtg tctttactga
    26581 tggaaaaggt gaatctgatc cagtaactgg cgacacctgc cgtggtgaaa aacgcaccaa
    26641 agcattaatt gattgcctac aacccgatcg tcgcgttgag atcgaagtcg caggcacacg
    26701 aagcgatcgc tacgaaacca ttgaacagaa ataaactcaa gtaaaatagt gagtagctta
    26761 agccctgcct tgtgcagggc ttttttatga gtaagtataa acgtgacatc tacagccttg
    26821 aggattgata cattactcga agcacgctgg attatcccag ttgatccctc ccatacaacc
    26881 ttaattgatc acgccattgc gatccaagat ggcaagatca tcgctattct tccaaccact
    26941 gaagcgctat cgcaattctc acctaaacaa cattttgttt taaatcatca cctcataatt
    27001 ccaggtctga ttaacacaca cacacatgct gcgatgacct taatgcgcgg catggctgat
    27061 gatctcccgt taatggattg gttaaaaaac tgcatatggc ccgttgaaaa acaatgggtt
    27121 gatacggatt ttgttctagc aggaacaact ttagcgtgtg cagaaatgct gaaaggaggc
    27181 attacttgct tcaatgacat gtatttcttc cctgaaaagg cggtggaagc ggccgctcaa
    27241 accggtatgc gtgcggcgat tggacttatc agtattgaat ttcccactgc atatgcgacg
    27301 gatgcaaatg attatctcgc caaaggactc atgctccgtg agcaatactt agaatatcct
    27361 actctctctt tctgtttagc gccgcatgcg ccttataccg ttagcgatag cacactgtct
    27421 aatatcttca cgtttgccaa tcaactggat ctaccaatcc acatgcacct gcatgaaacc
    27481 agcgaggaaa ttgcaagcag tctccatgat tttggaattc gtcctatcga gcgtttacaa
    27541 aagcttaatt tacttgggcc taatctgatt ggcgtgcata tggttcattt aaccaatgaa
    27601 gaaatcaact tattgaaact gcacaattgc aatgtagttc actgcccttc ttctaatatg
    27661 aagcttgcga gcggtattgc gcccatcgtt gacttagtca acgcatcgat caatgtcgca
    27721 attggaacag atggagctgc tagtaataat cgactcgata tgtttgaaga aatgcgcctc
    27781 acatcgttac tcggaaaagt cactcacggc gctcaggcac tgcccgctca tcaagtatta
    27841 cgaatggcga cgttaaatgg tgcgattact ctgggtattg aagcgctaac tggctcatta
    27901 tcggttggta aagctgcaga cattactgca atcgattttt ctggcttaga gctatcccct
    27961 tgtttcaatc cgctttccca cctaatttac gttgctggcc gagaacacgt cagccatgta
    28021 tgggtaaacg gtcaactctt gctgaacgaa ggagaacttg ttactctgaa cgaagcagaa
    28081 attatcgagc gcacgcaata ttggcaaaac cgcatcacaa gtaaacaata aaaggagaaa
    28141 aatggataat aatggtatca atgcagatcc attagagctt gaaaaattta gccaactcgc
    28201 tcaccactgg tgggatccca atagtgaatt caaaccattg catgaaatca acccactcag
    28261 gctgaattat attgatcaag tcagtggtgg tattagtgga aaatctgtag tagatgtcgg
    28321 atgcggtggc ggaatcttat cagaatctat ggcagctcga ggtgcaaatg tcaccggaat
    28381 tgatttaagc gataaagctt taaaagtagc taaattacac ttattagaaa gcggccatca
    28441 ggtcgattat cgtaaaatta ccgtagagat gcttgctacc gagcatcctc ggcattttga
    28501 cgtcgtaacc tgcatggaaa tgattgaaca tgtacccgat ccagccagcg tcattaagtc
    28561 ctgcgctcag cttgtgaaac ctggtggttg ggtattcttt tctaccctca atcgcaaccc
    28621 caaagcctat ctttttgcaa ttatcggggc tgaatatatt ctgaagttat tgccacgcgg
    28681 aacacacgag tacgctaaat ttatcaaacc ttctgagctt gctcatatgg ctcgtgacgc
    28741 tggattgact gaggccagaa taattggcat gacctataat cccatcacaa aaatttatgc
    28801 gctggaatct gacacgagtg ttaactatat catggctttc caagcctaat taatgcttta
    28861 aatcttacca aacactgcca acaacacatg gtcgacgcta tcctatttga ttttgatgga
    28921 acaattgctg atacagcacc tgatttaggt catgctctca atcgacaacg cataaaccgt
    28981 ggcttaccgt cgctcccgat tagcatcatc cgtaaacaag cttctgctgg atcccgcggt
    29041 ttactctacc ttggtttcaa tctagtgccg actcatcccg aatatgaact catgcgcgaa
    29101 gaatttctac agttctacac cgaacgtctt tgtcacgata cttgcttatt tccgggtatt
    29161 gcagatattt tagatcatct tgattcaagc aatattccat ggggtattgt aaccaacaaa
    29221 ccctctcgat ttaccaaacc catcgtgcaa tcgcttggcc tcataaaccg cgctgcctgt
    29281 attatcagtg gagatgatac accgcatacc aaaccgcacc cagaaccact actgcatgcc
    29341 tgcaaccaaa tcaacgctaa acctgcaaat tgtatttatt taggtgatga tatacgtgac
    29401 gtgaaagcga gccttgcggc tggaatcaaa ccgattgtcg cccgttacgg ttacctcggt
    29461 aacgatggtc cgcctgagtc ctggggcgca gatcacttga tcgatcaccc caaagagctg
    29521 gcccattatc tataaaaaca tattatggta ttgcgtgaaa agatacttgg taaagatttt
    29581 caacaatacg tcagtcaatt taaacacaaa ctttgttagt cattgattta ataaactccc
    29641 tatcagtaga ataactttag cacgcaacct ggattaatct atcctcacgg tgaatttctt
    29701 ggcagatttt catcaacaat ttgataatct cagttttgtt aatacaaata acactttata
    29761 ggaagcttga catgcatcat gtaaaaaagt ggttttgcta tttcgctatt ttttacttat
    29821 tcattgattc ctgcgttgct gactcgactg cccctgagca tgttcaaggt gctcagacaa
    29881 tcgacacctt aactgcaaaa ttattctttg atcaaaaaat tcctttcatt gatgtgcgta
    29941 aatacgaaga cttcattgcg gggcatattc ctggcgcgta tcatttgtca atcaaaagtg
    30001 atttcgatga actaagatta cgcgcgattg tagataaaga aagtccggta gtaatttatt
    30061 gtaatgggat ctattgcatg ggcagttcga tagctgcgca gaaagcagtc aattggggct
    30121 ggaagacaat ttattattat cgtgaaggaa tgccagcgtg gaaaaataac gagtacccca
    30181 ttcaggcaca gcaaaattaa tttgaattcc taaatttttc atcgataacc tcattgcgta
    30241 ataaatacag gcgtttgtta tgagtaaaaa gaaaattttg atgctagtgg gagactatgt
    30301 ggaagattat gaagcgatgg ttcctttcca agcgctacaa ataattggac acatagtgca
    30361 tacggtatgc ccagacaaaa aatcgggaga taccgttcga acagcagttc atgatttcga
    30421 aggtgatcaa acttactcgg agaaaccagg ccacttgttt cgattgaatg cgagcttctc
    30481 caaaatcaaa gtggaacaat acgacgcatt gatcattccg ggaggacgct cacccgaata
    30541 tatccgactg aaccccaaag taatcaagat tgtgcagcat tttgctcagc agggtaagcc
    30601 aatcgcggct atttgccatg gcgcacaatt attagtagca gccgatagtg tgcgaggtag
    30661 aacatgctct gcctatccca ccttggagcc agacgtcaaa cgcgcgggtg ggaaatatat
    30721 cgatattcct atggacaaag cgcatcaaga tcaaaatttg gtaacggcac ctggatggcc
    30781 tgcccatcca gattggctcg caaagtttct agatctacta gggacgaaaa tcaaacctta
    30841 gaaattatag gaaagtatta ctatgctttt tattacgagc cgattgccaa ctatcaatac
    30901 agaacctgaa ttgaataaaa attttcaatt caatttaggt gacaactcat caggtcgtga
    30961 gttattttgc tgcaagcgtc ttaaaaaaga cctctatgaa gaaatcggta gtaaaaatct
    31021 catgcaggcg attaaagagt caaaatatcg tcaagtatta atttatatcc atggtttctc
    31081 taacctccct gaagccgtgt tcaaggatgc tgaggaatta caattgttat gcaataagaa
    31141 gaaagcagat gaagtgctca ttcttccaat aatttggcct tgtgacaatg acctgggtat
    31201 tgttaaagat tattgggacg atcaaaaatc tgcggatcaa agttcatttg cattcgcgcg
    31261 catgctgcag aaatttatgg aatggcgaaa ttcacctgaa tacaatttga aaaataatca
    31321 ttgtttgaaa cggataaatg tgctggccca ttcgatgggg aatcgcgtat ttcgccaaac
    31381 cttagtgaat tggagaaaat atgatcaacc acaagggtta cccctactat ttagaaattc
    31441 ttttttggta gcggctgata ttttgaatga atcactacat aaag
//
LOCUS       DAOQKQ010000004        29261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_629_length_29261_cov_5.752277, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000004 DAOQKQ010000000
VERSION     DAOQKQ010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 29261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29261
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_629_length_29261_cov_5.752277"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..1385)
                     /locus_tag="PKZ49_00490"
     CDS             complement(186..1385)
                     /locus_tag="PKZ49_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HND35065.1"
                     /translation="MNFIESLQFALHALSAHRGRTILSASGIAIGIAAVLLLTSIGEG
                     IQRFVISEFTQFGTNILNITPGKIRAHGGSVGSIGSARILTIEDALALKTSRYVQYIN
                     ATVFGNAEIRAEGLSRRVTVYGQGPDFDKAFNMNVAIGQYLPTDDPRNPRAFAVLGAK
                     VHQELFGTMSPLGAILQVGSSRFRVIGVMAAKGQVLGFDLDDTVFIPTARALEVFNRE
                     GVMEIQAVYPPEFPVNLVTEDLQRILIERHGREDFTITPQKQMLSTLSTVLKVLTFAV
                     AALGGISLLVGAVGMVTLMHITVTERVAEIGLLNALGATRARIRTLFLLESTILSTIG
                     GLAGLIIGASIAFLLKIFVSALPVNIPWQFVSIALGISTLIGLIAGVFPAIQAARLNP
                     IDALRSE"
     gene            complement(1382..2584)
                     /locus_tag="PKZ49_00495"
     CDS             complement(1382..2584)
                     /locus_tag="PKZ49_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HND35066.1"
                     /translation="MKLSDILQFSLNTLRSYPIRSFLIVLAMALGVAAVVVLTALGDG
                     ARRYVIQQFSSIGTNLIVVLPGRAETAGSFPGAAMGQTPRELTLEDARWLGYIPQIRR
                     YAPLNVGAAELAGAGRLREVTVLGSTVDLLPIRHMELAQGGFLNQGSENNAQIVLGAK
                     LAKEFFPDGNAVGQRVRLADRRFIISGVLASQGETMGFNSDEIVIIPIYHAQALFNTT
                     SLFRILIEVRNRSDIPTTKQAIQEMLIQRHDGEDDVTVITQDAVLSTFDRILNALTLG
                     VAGIAAISLAVAGILVMNVMLVSVSQRTAEIGLFKAIGAQSHDIRYLFLAEATWLSLV
                     GAIIGFILGQFGSLLIRIAYPLLPAWPPIWACIAGIVVAISTGIIAGILPAVRASRLD
                     PVQALSSK"
     gene            complement(2581..3252)
                     /locus_tag="PKZ49_00500"
     CDS             complement(2581..3252)
                     /locus_tag="PKZ49_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HND35067.1"
                     /translation="MIELLGICRTFQMGDQAIHALNQINLKITAGEYVSIMGPSGSGK
                     STLLNVIGLLDQPNSGRYLLDGSDVTHLSESEQARVRRDKIGFVFQSFHLIPRLTAAE
                     NIELPLILSGITPSERKQRVEAALLAFNLVERAQHRPAELSGGQRQRVAIARATVLRP
                     TALLADEPTGNLDHRIGSEVIALLENLHHSGTTLIIVTHDRELGLRAHRRIAMRDGSI
                     IADEQ"
     gene            complement(3249..4403)
                     /locus_tag="PKZ49_00505"
     CDS             complement(3249..4403)
                     /locus_tag="PKZ49_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HND35068.1"
                     /translation="MQKNKIISRLLIILVTIMVAGYIGWHLSRKPPVEVELATASHGT
                     VEAIVVNTRAGTIKACRRAKLAPAAGGQIVKLLVKEGERVVQGQRLLELWNADLAAQH
                     ELAAQQLATTKSRQRENCIIANNAQRESIRTQQLVAKGFVSSQRAEDAEANAQAQQAS
                     CAAAGADVKRAQAQLEVTQANLNRTLLNAPFSGVIAQVTGELGEYTTPSPPGIPTPPA
                     IDLIDDSCLYVSAPMDEIDAPKLAIGQKARITLDALTNQTFAGVVRRIAPYVTEIEKQ
                     ARTVDVEVNFVENSNQVLLVGYSADVEVITDQREQVLRIPTQAIRQRNKVWALGPQDR
                     LEERTLEIGLSNWAYTEIISGLQAGDQVLIATDIETIVPGIQVKPKGPPS"
     gene            complement(4472..5026)
                     /locus_tag="PKZ49_00510"
     CDS             complement(4472..5026)
                     /locus_tag="PKZ49_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HND35069.1"
                     /translation="MPRKFFKKYLPSHESIQQNRFISFFGELLHHHNLWHLHRRSVAG
                     GFAAGLFAGLIPGSNPVQFFFAALFSITFKVNLPVAALTTLYSNPFTILPLYWTAYLL
                     GGFFIDNGMNNLPPLELNLADKSFSEWMPAVVTWLTSFGKPLIIGLVLLASLLAIIGY
                     FLVRLIWRFYVIHAWHKRHQRPQP"
     gene            5182..6834
                     /locus_tag="PKZ49_00515"
     CDS             5182..6834
                     /locus_tag="PKZ49_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HND35070.1"
                     /translation="MTEVTDSTPTAIFRTPYILSNRKQPAEIKGTRPTETEYGVLDPK
                     TFDAVEVDALFEGMNHAETQIGQAVLYRSLAQPTHHVEQLHKKQEALRELAMNPPLLD
                     ACEQFIKKMADGEETLYHLLYGEFSGGFTTDHPKNRVGKLEFGGYGYRQFIDGTEFAV
                     DLVEEAGELPPVQSAYLQELLQAIRDFGGSKTYALMHGPFYVIDGKFKTKDEKPPYLP
                     IPRFRPSMFKWLPSLIFVLIVSGLLYFFENMLVELGANYIGYGILILTVPIFPIIVQA
                     VSASDRDSVIYPLQKLFRQSSELAKAMEALGMLDELLSLHRYAKASHHEMVLPEIMTA
                     GQHTLTAIQAKNPLLLNTNTHYVANDVKLDAANRLLIITGPNSGGKTAYCKMIVQLQI
                     LGQAGSYLPASMARIVPAEHIFYQIPDAGKLEEGMGRFAHELKQTREIFFNSTSLSLV
                     ILDELAEGTTFEEKMTLSEYVLKGFHQLGASTLLVTHNHELCERLLSEGIGSYLQVEF
                     LQGEPTHRLIPGISRVSHADRVASAIGFSKADVEKHLESLKS"
     gene            complement(6852..9116)
                     /locus_tag="PKZ49_00520"
     CDS             complement(6852..9116)
                     /locus_tag="PKZ49_00520"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HND35071.1"
                     /translation="MSNLPTDRSPTAFDYSLNQDEDVINLGELFGTLYESKWLILLIM
                     TVTVLIGFAKTQLDRPIYKADGLLQVKENSTSMAGLEPLAGLIEGKMAVMAEIELIKS
                     RMILGKTIKSLHLDVIAKPRYFPIVGETIARQFQNRNQDGSVSNPLFGLTQYAWGGET
                     IQVETFIVPSILEDEEFILKTDQEGFFKLFYKDEIILEGEVGKLANKQIEGYSQPIAI
                     FVSLLKSRPETHFTIVRQSENQAIRQLKERISIAEKGKNTGMLELTVESSLPDAAVSV
                     LNEIANIYVQQNVDQKSAESQKTLEFLEKQLPILKDQLDAATTSLNEYRTRKGSVDLN
                     LEMQNILKNSVELKTQTTILQQKRDELRQRFTESHPHIIAIDKQIARLQGQISTQDKK
                     IETLPETQQAILKLSRDVKVSTELYTTLLNNAQTLRIAKAGTIGNVRIIDYAILPDEP
                     IRPRKVLIIGIALVLGIIFGVVTALIRKSLNRGIEDPDLIEKLLNTPVYATIPHSKFQ
                     KALSNKLKHNQANNVASPVILALENKEDVAIESLRSLRTTLHFAFLEAQNNIIMITGP
                     SPGIGKTFVSVNLAVVMADAGKKLLLIDGDMRRGYINKVLGVNRENGLSELIFESISL
                     DKAIHPIESAHFDFIATGAIPPNPSELLLHERFANLLDNLSKQYDLVIIDSPPILAVT
                     DAAVIGRLAGATLMVIKAGQHPARELEQSTKRLIQAGVNLKGIVFNDLPELASRPGYG
                     YGKYVYQYSYKSGK"
     gene            complement(9244..10476)
                     /locus_tag="PKZ49_00525"
     CDS             complement(9244..10476)
                     /locus_tag="PKZ49_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HND35072.1"
                     /translation="MHLKIFLVLAACALPACSIVPGQHMSPFSRQSSVKMPVTENNET
                     ILKTLDIQTINAELVLALERDFNTRSLGPDNVANHYFDYRIGSRTIKGVPMETEPYTQ
                     YRVGPRDILNITVWDHPELTIPAGQFRAAEKAGNVVGEDGTFFYPYVGIVHAAGRTVE
                     EIRDELTERLSRYIENVQLDVRVADYRSQRVYVVGEVAQPGIQLVKDIPLTVLEAINN
                     AGGVKSEADQRNITLTRDDKTYSINLLSLYEGGDITQNVLLKHGDILNVPDSSLNKVF
                     ILGETNFRVLGGAGIGRSRSLVMNKARLTLTEALSDAGGFDQETSDPARIFVFRGGVG
                     KSEIFHLDAKSPDALLLADRFPLQPRDVIYVDRAEGIRWNQIIAQIQPTVNLLNSFDG
                     VLRIQPFRAFPSAPSTVR"
     gene            complement(10591..11031)
                     /locus_tag="PKZ49_00530"
     CDS             complement(10591..11031)
                     /locus_tag="PKZ49_00530"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HND35073.1"
                     /translation="MFTNILLVCVGNICRSPMAEGLLKHHLAQSAQTNHFVTSAGLNA
                     LVGHKADEKACHVLIQKGIDISSHRACQLNSVMVRKADLILVMETSHKKAIEDNEPGA
                     RGKVFRLGEWGKFEIADPYQKDLSIFEDSALLIEQGINQWLTKL"
     gene            complement(11035..12138)
                     /locus_tag="PKZ49_00535"
     CDS             complement(11035..12138)
                     /locus_tag="PKZ49_00535"
                     /inference="COORDINATES: protein motif:HMM:NF018805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HND35074.1"
                     /translation="MKTKPKSNHSLASVLMKMILIMMLGLGVWLAYEVFYLQPADNWF
                     EVKAKTSSSVNLPKDDAPHLAKMEWWYYNGHLWTESGKQFSFHYTTFMVTELVSHLVK
                     QVSLSDHQTGKHYTDQKRTTGGGFSSNIENGFEFLLDNWKMVGDDGNDQLIVSTPQFS
                     FNLKLTNTIPPVFHGTDGIIFLEEAGNSYYYSRTRMDITGTIKIGNTTETVRGLSWFD
                     HQWGDFSTNLLSWDWFSLQLDNGIDLMIYQLRDKYDKPLLYTGSITQNNVTEALLSSD
                     FTLSQLGDQWTSKKTGYTYPIAWEIKIPKKNIHIKTRSKVENSEFDAQLTTYNHYWEG
                     AVKIEGTHTGKGFMELYGYTNKKEPNASRSKKR"
     gene            12536..13282
                     /locus_tag="PKZ49_00540"
     CDS             12536..13282
                     /locus_tag="PKZ49_00540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HND35075.1"
                     /translation="MNIKYQIKNLLLATSITIGLSATSAMANVVGFMGLELDGCFSSN
                     ANTAVCGPHENNAAVFATGTAVFNESLLSRFGGTAGLATQPFSYAGFGELLVGTSPGA
                     PLAPFADITRTWADYTALSSDPNLMLGFAILNQIAAHPNDASSSFTFDLTPNPGDEFS
                     IFWSITNIVTNGSVTTGDWKAWSDDVLPLNGLSMALFGSALPSDVNFNLNLTLNAIPE
                     PVTLALVGLGILGMTAVGRRRSNTSNISFA"
     gene            13378..13689
                     /locus_tag="PKZ49_00545"
     CDS             13378..13689
                     /locus_tag="PKZ49_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HND35076.1"
                     /translation="MTSVIYRITYPNNKIYIGQDRTNSINYFGSASSELISQDFTNEQ
                     RQSFTITRDILWSSECASQSEVTHVEYELIERYHANNPAVGYNQFPPFKKTNYEVKKE
                     S"
     gene            complement(13697..14323)
                     /locus_tag="PKZ49_00550"
     CDS             complement(13697..14323)
                     /locus_tag="PKZ49_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HND35077.1"
                     /translation="MENEQPPQQSQHNTSDLLQLVVVQNVNDKLSIGEIKNMLHERGF
                     GVLLVIAAIPICLPIPAPPGFTTVFSLPLIIFSLQMICGMRSPWLPRWLTDKKISRKG
                     LEKLVQKAAPWLRKIESYLHPRITYISVSAWERIIGIFVLIFSITIALPIPMINFLPG
                     WGVMIMSLGLLSKDGLVIIGGMIVGTIGVGIALIAVILIWAGVAPAPL"
     gene            complement(14429..15946)
                     /locus_tag="PKZ49_00555"
     CDS             complement(14429..15946)
                     /locus_tag="PKZ49_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HND35078.1"
                     /translation="MSQSSTNIHKRLKSLEKHLADEHPNNPVLAKAVHSFRKLDRVAQ
                     DMGLLDHNESYATYVTWWPMIAVLGTFSAGKSTFINSYLNLNLQRTGNQAVDDKFTVI
                     CYTDQDEVKTLPGVALDADPRFPFFQISRSISEISETGPQRIDAYIQLKTCPSEKIRG
                     KILIDSPGFDADSQRTSTLRLTQHIIDLSDLVLVFFDARHPEPGAMKDTLEYLVSGTI
                     NRADANKFLYILNQMDVTAKEDNPEEVVSAWQRSLAQVGLLAGRFYRIYDPKAANPIN
                     DAQVKERFEKKREEDMADINARMQQIEVERSYRIAGMLEQTAETIETQVVSKLTDMLD
                     QWKKSVFLFDGILFTLLFIACGVILTMTESWGILQPFAENVIAQDTQSLIILGIMVSI
                     IGYIHFSIRGAVAKSLVKKLPAEFGHDHNSCKQYTQAFLKCTSAFRPMFLSKPSGWNS
                     SNQQILAEVKDEANDYIQSLNDLFTDPSGRKADAEAVNEPVIDEQQNQPTQQASQ"
     gene            complement(16110..17018)
                     /locus_tag="PKZ49_00560"
     CDS             complement(16110..17018)
                     /locus_tag="PKZ49_00560"
                     /inference="COORDINATES: protein motif:HMM:NF015818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HND35079.1"
                     /translation="MNNAKKPLTTPWLDITQQIRQFDRNFNILAIDTIGGGCINQTYR
                     IQGDHCDYFVKLNKASFLPSFEAEAAGLTEIQETHTIRVPEPKCYGYNTANAWLVLEY
                     ISLNPVNKNGYLILGQNLAALHRITSQQFGWFRDNTIGATLQKNNHTANWVDFWRHQR
                     LGYQLDLAKQKGYGGKLQQLGEKLLTDLDLFFPSQSIVPALLHGDLWSGNCASDQSGQ
                     PIIYDPAVYYGDRETDLAMTELFGGFPSQFYAAYQEAYPLDKGYNIRKTLYNLYHILN
                     HLNLFGGGYLRQAEQMQVKLLAELRS"
     gene            complement(17024..18406)
                     /gene="argH"
                     /locus_tag="PKZ49_00565"
     CDS             complement(17024..18406)
                     /gene="argH"
                     /locus_tag="PKZ49_00565"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HND35080.1"
                     /translation="MKKHTNTWSGRFNEPVSELVQRYTASVNFDKRLAEYDIQGSLAH
                     AQMLASVGIISDADLVAIEQGLTTILQEIRDNTFDWKIELEDVHLNIEKRLTALIGDA
                     GKKLHTARSRNDQVATDIRLYLRASIDEITSLIQAVQLALLNLAENHIDTIMPGFTHL
                     QVAQPISLGHHLMAYHEMLARDRERLTDCRKRLNRLPLGAAALAGTSYPIDRMFVAKQ
                     LAFDRVCSNSLDAVSDRDFAIEFCACAALIMMHLSRLSEEIIIWMNPLFNFIQLADRF
                     CTGSSIMPQKKNPDVPELVRGKTGRVNGHLIALLTLMKSQPLAYNKDNQEDKEPLFDA
                     IDTLTDTLRIYSEMIPGITVNHEAMRNAAMRGYATATDLADYLVKKGIPFRDAHEMVA
                     QAVRFAESKSCDLSKLTLTELQQFTPLIEEDIFSVLTLEGSIKSRNHVGGTAPDQVRQ
                     AIAQARHDLT"
     gene            18647..19303
                     /locus_tag="PKZ49_00570"
     CDS             18647..19303
                     /locus_tag="PKZ49_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35081.1"
                     /translation="MTDQYKQNDSKNTINWTNPDALLNMLSGKFAAFDKFQKMQLPSW
                     SVWVFLGILLLVIAWSQIVVSQANSRLEKGQAEIANQLELERSTLIRKAREYADAQYH
                     KEEDRFGQVLSWAVRGELLRNNLDQVSQYLNELVKIKDTERAVLIGDDGKLLVSTDKK
                     LEEAAAKEIYPEELLQQRKITIQSDVNGRKLLIVPIMGLNNRLATVVISYKNPPLNSN
                     "
     gene            complement(19364..20617)
                     /gene="murA"
                     /locus_tag="PKZ49_00575"
     CDS             complement(19364..20617)
                     /gene="murA"
                     /locus_tag="PKZ49_00575"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HND35082.1"
                     /translation="MQKLLIQGGYPLQGEITISGAKNAALPLLCASLLTTDTIELSNV
                     PELMDIVTMLSLLQQMGVATRRKDHSKIELSTKDVSNPTAPYEMVKTMRAAILVLGPL
                     VARVGEAHISLPGGCAIGLRPVDQHIKGLQAMGAEIAIEHGYIRAQASKLRGARILMD
                     IVTVTGTENLMMAATLASGLTIIENAAREPEIIDLANCLNQMGAKISGAGTDIITIEG
                     VEALHGVSYEVMPDRIETGTFLTAATATTGEILLKKARAETLDAVTSKLLEAGALIDF
                     DRDQIHLVMNKRPQSVSLRTAPHPAFPTDMQAQFMALNAIAEGTALITETIFENRFMH
                     VQELKRLNADITVEGNTAVIKGIQQLDGANVMATDLRASASLVIAGLVARDETTIDRI
                     YHLDRGYESIEKKLSSVGAQIKRVT"
     gene            20750..21139
                     /locus_tag="PKZ49_00580"
     CDS             20750..21139
                     /locus_tag="PKZ49_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HND35083.1"
                     /translation="MAKQIIQTVDAPQAIGTYSQAVRVNGGDTIYLSGQIGLDPVTMQ
                     MVEGIDAQIERVILNLKAVAKASGGSLSDIVKLNIFLIDLANFAKVNEAMAQHFQQPY
                     PARAAIGVASLPRGALVEMDGVMVLSN"
     gene            21185..23251
                     /gene="recG"
                     /locus_tag="PKZ49_00585"
     CDS             21185..23251
                     /gene="recG"
                     /locus_tag="PKZ49_00585"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HND35084.1"
                     /translation="MITIHHSIFSRIEQKTQQKLAKLSIQNDTDLILHLPLRYEDETN
                     LYLINDVIDGNVFQVEGEVIHNEIIYRPRKQLIVQISDGSGTLLIRFFNFYPNQIKLY
                     SVGVRIRALGEVRRGFWGIEMVHPKCRIVQNETPLADTLTPVYSTTAGLSQPSIRKWI
                     QQALTRMDHQELLCEALPQEILSRYQLLEFKASLFVLHKPLAGISVTSLQERKHPAWR
                     RIKFDELLAQQLSMRLHYQQRRNNLAPVLTPKQSLTDQLLCALPFCLTAAQEKVLVEI
                     FHDLAAQQPMQRLLQGDVGSGKTIVSALAALRAIENGYQVAIMAPTEILAEQHHSRFI
                     SWLAPLDIEVAWLSGSQTKKQRERELARISTGKAMLAIGTHALFQDQVAFSRLGLVII
                     DEQHRFGVAQRLALKHKGNKKALVPHQLMMSATPIPRTLAMSYYADLDVSTIDQLPPG
                     RAPIVTKLIDNSRRAEVTQRVYEACNTGKQIYWVCPLIEESEALELKTAIETYEALSQ
                     HLSDIKVGLIHGRLNASEKSSIMTDFSQGNIQLLVATTVIEVGVDVPNASWMIIEHAE
                     RLGLSQLHQLRGRIGRGQEASLCILLYQKPLSKIARERLRIIFEHNDGFEIAQQDLRL
                     RGPGELLGTRQSGIPLLRFANLEEDKDLLEAARNVAEQLLNENPKVVEFYLTWWLASR
                     NVLLQA"
     gene            23350..24765
                     /locus_tag="PKZ49_00590"
     CDS             23350..24765
                     /locus_tag="PKZ49_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HND35085.1"
                     /translation="MKILYHRQSINLLLRWVFSLFFLLMVTACALGPDYRRPAIEVAE
                     HYRIPHAEGESIANLPWWELFQDKVLQQLIQQALQENKDLKRVISSIEEYQARLSMAN
                     MDFAPQLDTDITAPVMGKIGGFSRPGFATPFNYFGMVNLNWEIDIWGRIRRSTQAAFA
                     ELIAQEANRRAIVLSLVGAVAQSYFDLLQLDKQLELAQQILHSWEESVAISDAQLQHG
                     LISKLDNDQFQAERANAAARVAILEREMVQKENELSLLLGKSPNSVLRGSAINEQWLS
                     PEIPAGLPSELLDRRPDILRDEQVLAAATARIGATKAARLPRISLTGFLGVASPSLSN
                     LLLSNSQFGVGGLGLAAPILNARALGFEQKAVEAQAKQALAQYEQTALIAFKEVEDAL
                     VAIRTVKDQYKATQEQVKALQSALQIADLRYQSGLTSYVDVLLAKRSLFDAETALVST
                     QRFHLVALVQLYKALGGGWAP"
     gene            24889..26109
                     /locus_tag="PKZ49_00595"
     CDS             24889..26109
                     /locus_tag="PKZ49_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HND35086.1"
                     /translation="MYPLISWVFRGTRQIKLQVIYVGLGLFVCLAIGCSDKPATDLAD
                     SPLPQVAVITLKTQSVADEPEFIGQTQSFRPVEIRSQVSGIIKQIFFTEGRNIKKGDR
                     LYQIDPIPFRAAYQRGQANVAQAKVRLKQAQQDLARVRPLLEKKAVSKKNIDDAEAEV
                     QAAKATLDAAQNELTKAKFDLDNTLIYSPVNGRIGRSQFYEGRLIEAQESLLTAIAQL
                     DPMYVNVNVPESYLLKRRRELVEQKVASPELFQLRGEMTFSDGTVYPEEGVLDFADIR
                     LRPETGTLQGRFRFSNPDGKGEPSVRNFLSGQFVKVRVKGYIRHNAILVPQRAVHQGP
                     KGSFVFIINEEDKVELRSVLASSWRGKEWLIESGLSAGERVVVEGFFRILPGVKVNAV
                     AYSLIENESASRHD"
     gene            26134..>29261
                     /locus_tag="PKZ49_00600"
     CDS             26134..>29261
                     /locus_tag="PKZ49_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HND35087.1"
                     /translation="MNSHFFIDRPIFASVLSIIIVVIGLVSLQTLPVAQFPQITPPMV
                     QIDADYPGASAEVVAEAVARPIEVQLPGIDNLLYYESTSTNDGHMMMRLTFEIGTDID
                     IAQVQALNRQRLAEPQLPAEVIRQGITVKKTSPDLLAVVALSSTDPQHDTVYLSNYAL
                     LRVLDNIKRLSGVGDAIIFGEQNYSMRIILDPIRMAQLNVTPSEIIAVVQDQNRDFPA
                     GRIGREPTAQGTELTIPVITHGRMSEVHEFEEIIIRAFPDGSMLKLKDVANIELGARS
                     YDLQGRWNGKPNTFLLTFLAPGANALETVERVRAEMERLAKMFPAGVAYDIPYDTTKF
                     IEVSIEEVVKTLAEATLLVLLVVFLFLQNWRATLIPAVAVPVSLIGTLIGMSALGFSI
                     NTLTLFGMVLAIGIVVDDAIVVVENVERHMNQGGLSSRDAARKAMDEVTGPVIAIVLV
                     LSAVFLPVAFLSGITGELYKQFAITIALSVILSGFVALTLSPALCALVLKPSHNVSSN
                     LFWKLFNQSFSWLQTRYVGVVSIALKRWVIALAIFLALMVIIVGLFNRIPQSFIPEED
                     QGYFITVVQLPDGASLTRTTAVLDKVESFFQSISAVHSTDTLAGQNFVFGTRGTNQAT
                     MFVPLNHWDTRKAEQDQIQTLIKRAYQEFATIPEALILAFNAPAISGLGSTGGFSAQI
                     QDPSGGDLVEFAAIAQEFVTKATEHPAIAIASTNFKISAPQFFIEVDRERAKSLGVSI
                     SEIFDTLQAYFGSWYINDFIKYGRVYRVQTEAMPEYRSNPDNLNHIHVRAQSGSSQTM
                     IPLSSVVTTQFNSGPDPVTHFNGFNSAMVLGSAAPGYSSGQALDALQAVADEILLPKG
                     YALDWSGISLQERKAGGQSLLVFAFALLMVFLVLAALYESWSIPFAVIFAIPLGILGA
                     LLAIWLRDLSNDIYFQIGLVTLIGLAAKNAILIVEFANQNYRAGQPLIDATLEAAKLR
                     FRPIIMTSMAFILGVFPLVIASGAGAASRHSIGTGVFGGMLAATFLAIFFVPIFFVIV
                     NRIAHRVRLRNTR"
BASE COUNT         8167 a         5925 c         6646 g         8523 t
ORIGIN      
        1 aagggagtaa tggccgctgg cgtgatgtgc tatcggagcc ggaattgctg ctttatgatc
       61 aagcttgtca gcgagcattg actcccgatt gccgagaatg gttggagaat gggggaagaa
      121 taatgtgatt tttttgtagc gataatctca ttgttcaagt tgaaatcatc gctgatcagt
      181 gtgaattact cgctcctcag cgcatcgatt ggatttagtc gtgctgcttg aatcgctgga
      241 aagacaccgg caatgagtcc gatcaaggtg gagataccaa gtgcgatgct cacaaactgc
      301 cagggaatgt tgactggtaa agcgctgacg aagattttaa gcaaaaaagc aatactcgcg
      361 ccaataataa gacctgctag gccaccgatt gttgaaagaa tggtcgattc caggagaaac
      421 aacgttctaa tacgggctcg tgtggcaccg agtgcattga gtaaaccaat ttcagcaaca
      481 cgttctgtca ccgtaatatg catgagggtg accataccca ctgcgcccac aagcaaggaa
      541 atgccaccga gtgcagcgac tgcaaacgtg agtactttca agacggtaga aagcgtactt
      601 aacatttgtt tttgcggggt aatggtgaaa tcttccctcc catgacgctc gatgaggata
      661 cgttgtaaat cctcggtaac gaggttaaca ggaaattccg gagggtaaac tgcttggatt
      721 tccatcacac cttcgcgatt gaagacttcc agtgcacgag ccgtagggat aaacacggta
      781 tcgtccaaat caaagccaag cacttgtcct tttgcagcca tgacaccgat tacgcgaaag
      841 cgtgagctgc cgacctgcag aatggcacct aacggactca tggttccaaa taattcctga
      901 tgaaccttag cgccgagcac agcaaaggcg cgagggttac ggggatcgtc ggtcggtaaa
      961 tactgaccga tcgctacatt catattaaac gctttatcaa aatcgggacc ttgtccataa
     1021 acagttactc ggcgactaag cccttccgca cggatttctg cgttaccaaa taccgttgca
     1081 ttgatatatt ggacatagcg cgatgttttc aacgcaagcg catcttcgat cgtgaggata
     1141 cgtgcgctac caatcgaacc gacagaacca ccatgtgcgc gaatttttcc tggcgtaatg
     1201 ttgagaatat tggtgccaaa ttgtgtaaat tcggaaatta caaagcgctg aataccttcg
     1261 ccaatggaag tcagtagtaa aacagcagcg ataccaatcg cgatcccgga tgcggataag
     1321 atcgtgcgac cgcgatgggc tgatagcgca tggagggcaa actgaagtga ttcgatgaaa
     1381 ttcatttgct acttagcgct tgcaccgggt caaggcgcga tgcgcgaacg gctggtaaaa
     1441 tacctgcaat gatgccagtg ctaatggcga caacaatacc tgcaatacaa gcccagattg
     1501 gtggccaagc tggcagaagt gggtaagcaa ttcgtattaa caggctaccg aattggccta
     1561 atataaaacc aatgatagcc ccaaccagcg atagccaagt ggcttcagct agaaacaaat
     1621 agcggatatc atgggattga gcaccaatcg ctttaaatag gccaatttca gccgttcgtt
     1681 gactgactga aaccagcatg acattcatga cgagtatccc tgcaacagct aaactgatag
     1741 cagcaatgcc agcaacaccg agtgtcaagg cattaaggat acgatcaaag gttgatagca
     1801 ctgcgtcttg ggtaataacc gttacatcgt cttcgccatc atggcgttga attagcattt
     1861 cttgtatcgc ttgcttggtg gttggaatat cgctgcgatt cctgacttca atcaaaattc
     1921 gaaacagtga agtggtgttg aacagagctt gggcatgata aatcgggatg attacaattt
     1981 cgtcagaatt aaagcccatg gtttcaccct gactggctaa tacaccagaa atgataaatc
     2041 gccgatcggc taaccgaact ctttgaccca ctgcattacc atctgggaag aattctttgg
     2101 caagcttagc acccaataca atttgtgcat tattttcaga accttgattg aggaaccccc
     2161 cttgagcaag ctccatatgg cgaatcggta gtaaatcgac cgtgctgccc aatacggtaa
     2221 cttcgcgtag ccgaccggca cctgcaagtt ccgcagcgcc aacattgagc ggtgcatagc
     2281 gccggatttg cggtatgtat cctaaccaac gagcatcttc caaggtcagc tcacgcggcg
     2341 tttgtcccat agcggcacca ggaaaacttc ctgctgtttc agctcgacca ggtagaacga
     2401 caattagatt ggttccaatc gatgaaaatt gttggatgac atagcgtcgt gcaccgtccc
     2461 ctagggcagt taggacgacg acagctgcta cgcccaaagc catggcgaga acgatgagaa
     2521 acgagcgaat cgggtagctt cttagcgtat tcaatgaaaa ttgtaggata tcggagagtt
     2581 tcattgttcg tcagcgatga tgctgccatc gcgcatcgcg atacgacgat gggcacgtaa
     2641 tccaagctca cgatcatgcg ttacgatgat cagtgttgtt ccgctatggt gaagattttc
     2701 cagcagggcg atgacttctg atccaatgcg gtgatcgaga ttgccggttg gttcatctgc
     2761 caataatgca gtagggcgca gaactgtagc acgggcaatt gcaacacgct gccgttgccc
     2821 gcccgataac tctgcgggac gatgttgcgc acgctcaact aagttaaatg ccagcaatgc
     2881 agcttcaacg cgctgcttac gctcactggg agtaataccc gataagatca gcggcagctc
     2941 gatattttca gcggcggtca ggcgggggat gaggtggaat gattgaaata caaaaccaat
     3001 tttatctcgg cgcacgcggg cctgttctga ttcggataaa tgagtaacat cgctaccatc
     3061 taaaagatat cgtccgctat tgggttgatc gagtaatcca atcacattga gcaatgtcga
     3121 ttttcctgag ccggaaggtc ccataataga aacatattca ccagcagtaa tttttagatt
     3181 aatctgattc aacgcatgaa ttgcttgatc gcccatctga aaggtacgac agatgcctaa
     3241 gagctcaatc acgatggtgg gcctttaggt tttacttgaa tgccgggaac aatcgtttca
     3301 atatcggttg cgattaatac ttgatcacct gcttgcaagc cgctgatgat ttctgtatag
     3361 gcccagtttg aaagcccaat ttccagcgtg cgttcttcaa gtcgatcctg aggaccgagt
     3421 gcccatactt tattgcgctg ccgaatcgct tgagttggaa ttcgtaatac ttgctcgcgc
     3481 tggtcagtga tgacctcaac atctgcactg taaccgacta acaacacttg gtttgaattt
     3541 tctacgaaat tgacttcaac atccacggtt ctggcttgtt tttcaatttc tgttacataa
     3601 ggggcaatac gccggactac acccgcaaat gtttgattag ttaatgcatc gagcgtgatg
     3661 cgtgcctttt gcccaatggc gagtttgggc gcatcgattt catccattgg ggcgctgaca
     3721 tagaggcagg aatcatcgat taagtcgatt gcaggtggtg ttggaatgcc aggaggagag
     3781 ggggttgtat attcaccgag ttccccagtt acttgagcaa tgacacccga gaacggtgcg
     3841 ttgagtaggg tacggttcag attggcttgg gttacctcga gttgtgcttg agcgcgtttt
     3901 acatctgcac ctgcagcggc acaactggct tgttgtgctt gtgcattggc ttcagcatct
     3961 tcggcacgtt gtgaactgac aaaccctttt gcaacgagtt gctgggttct tatggattct
     4021 cgttgtgcgt tattggcgat aatacaattt tcccgttgac ggcttttggt tgttgccagt
     4081 tgttgtgctg caagctcatg ttgcgcagca agatcagcgt tccacaattc cagtaatcgc
     4141 tgtccctgga caacccgttc tccttctttc actaaaagct taacgatttg cccgcctgca
     4201 gcaggtgcga gttttgctcg tcggcaagct ttaatcgttc ctgcccgagt attgacgaca
     4261 atcgcttcca cagtgccatg actagcagta gctaactcta cttcgactgg cggtttacgc
     4321 gacaggtgcc aaccgatata gcctgcgacc atgatggtaa caagaatgat aagtaaacga
     4381 ctgataattt tatttttctg catgatgagt gatgtaaggc ctaagcatgt agcccgttta
     4441 ataattgata ttttagatta aatcaaaatg tctagggttg ggggcgctgg tggcgcttat
     4501 gccaggcatg aataacatag aaccgccaaa tcagtcggac caaaaaataa ccgatgatgg
     4561 caagcaatga tgcaagtaag accaatccga tgatcaacgg ctttccaaac gaggtgagcc
     4621 aagtcacgac agccggcatc cattcagaaa aagatttgtc agctaaattg agttccaatg
     4681 gcggaagatt attcatgcca ttatcgataa agaaaccacc cagtaagtac gcggtccaat
     4741 agagcggtag aatcgtaaat ggatttgaat agagtgttgt caaagcagca actggcaaat
     4801 tcactttaaa ggtgatggag aataaagcgg caaagaagaa ttgaactggg ttactaccgg
     4861 gaatgagtcc tgcgaataac cccgcagcaa agccacctgc caccgatcga cgatggagat
     4921 gccacagatt atgatgatgc aacagctcac caaaaaaact gatgaaccga ttctgttgaa
     4981 tgctttcgtg agaagggaga tattttttaa aaaattttcg aggcacgggt gtgttttctc
     5041 aaaatactat gcactatgga gtattaaaaa taagttttgt ttcatgtcag caattaatga
     5101 gtggtatttc tcgttatatt gcagctaaac gctgtgagaa ataacatata gcaatttata
     5161 tacataattt gaaggtggtt tatgacagaa gtgactgact caaccccaac cgctattttt
     5221 agaacaccct atatcttatc aaatcgtaag caacctgctg agataaaggg gacgagaccc
     5281 actgaaacgg aatatggtgt gcttgatccg aaaacatttg acgctgtcga agtggacgca
     5341 ctatttgaag gaatgaatca tgctgagact caaatcggcc aggccgtatt gtatcgttca
     5401 ttagctcaac cgacacatca tgttgagcaa ctgcataaaa aacaggaagc attacgagag
     5461 ttagcgatga atcccccgtt actagacgct tgcgaacagt tcatcaagaa aatggccgat
     5521 ggagaagaaa cgctttacca tttactgtat ggtgagtttt ctggtggatt cacgacagat
     5581 catcctaaga atcgggttgg taaacttgaa tttggggggt atggttatcg acaattcatc
     5641 gatgggactg aatttgctgt cgatttggtg gaagaagctg gtgagctgcc gccggtacaa
     5701 agtgcctatt tgcaggaatt gcttcaggcg attcgtgatt ttggcggttc aaaaacctat
     5761 gctttgatgc atggcccttt ttatgtcatc gatggtaagt ttaagactaa agacgaaaaa
     5821 ccgccttatt tacctattcc gcgattccgg ccgtcgatgt tcaagtggct acccagctta
     5881 attttcgttc tgattgtgag tggtttactt tatttcttcg agaacatgtt ggttgagctg
     5941 ggtgccaatt atattggata cggtatttta atcctgacag tgccaatttt tccgatcatt
     6001 gttcaagcag taagcgcttc agatcgagat tctgttattt acccgttgca gaaacttttt
     6061 cgacaaagct cggaattagc aaaggcgatg gaagcactcg gtatgctcga tgagctttta
     6121 tcgctgcatc gttatgccaa ggcatcacat catgaaatgg tcttgcctga gattatgact
     6181 gctggtcaac atacgctaac agcaatacaa gctaaaaatc ctttgcttct gaacactaat
     6241 acgcactatg tggctaatga cgtgaaattg gatgcagcga atcgattgtt gattattacc
     6301 ggccctaata gcgggggtaa aaccgcttat tgcaaaatga tcgtgcaact gcagatttta
     6361 gggcaagcgg gaagttatct acctgcatcc atggcgcgga ttgtccctgc cgaacatatt
     6421 ttttatcaaa tacccgatgc agggaaacta gaagaaggaa tgggtcgatt tgcacatgag
     6481 ttgaaacaga cccgtgagat ttttttcaat tcgacatcac ttagcttggt tattctggat
     6541 gaattggctg aagggacgac atttgaagag aaaatgacgt tgtctgaata tgttttaaag
     6601 ggatttcatc aattgggagc atcaacgcta ttagtgacac ataatcatga actctgtgag
     6661 cggctactca gtgaaggaat tggaagctat ctgcaagtag aatttttaca aggtgagcct
     6721 acccatcgat tgattcccgg tatttcacga gtcagccatg ctgaccgcgt tgcgagcgca
     6781 attggattta gcaaagctga tgtggaaaaa catctcgagt ctttaaaatc ctgacgaaat
     6841 ttgtggatgc gctatttgcc acttttgtaa gaatactgat agacatactt gccatatcca
     6901 tagccaggac gcgaggcaag ttctggcaag tcattgaaca cgatgccttt tagattgaca
     6961 cccgcttgga tcagtctttt ggtactttgc tccaattctc ttgcaggatg ttgccctgct
     7021 ttgatgacca tcagtgtggc gccagccaaa cgaccgatta cggcggcatc tgtaacagca
     7081 agaataggcg gagaatcaat gatgactaga tcatattgtt tgctaaggtt gtctaaaaga
     7141 tttgcaaaac gctcatgcag taataattcg gatggattag gtggtatggc gcctgttgca
     7201 atgaaatcaa aatgagcact ctcaatggga tgaatggctt tatctaaact gatcgattca
     7261 aaaatgagct cagataaacc attttctcga tttacaccga gcactttgtt gatatagccc
     7321 cggcgcatat caccatcgat cagcagcagt ttcttgcctg catcagccat aacgacggct
     7381 agattgacgg aaacgaaagt tttaccaata ccaggactgg gtcccgtgat cataatgata
     7441 ttattctgtg cttcgaggaa agcaaaatgc agtgtcgttc ttaagctgcg caagctttca
     7501 atggcaacgt cttctttatt ctcgagtgca agaataaccg gtgaagctac attatttgct
     7561 tgattatgct tcaatttgtt gctgagtgct ttttggaatt tactatgcgg tatcgttgca
     7621 taaactgggg tattcagtag tttttcaatt aaatcaggat cttcaatacc acgattgagc
     7681 gatttacgaa ttaatgcggt tacaacacca aagatgatcc ctaaaacgag cgcaataccg
     7741 ataatcaata cttttctagg gcgaatcggc tcatccggta gaatggcata atcaataatc
     7801 ctaacatttc caatggttcc cgcttttgca atgcgcaatg tttgggcatt gttgagcagc
     7861 gttgtatata actcggtact gacttttaca tctctggata atttcagaat agcctgctgc
     7921 gtttcaggta gagtctcgat ttttttatct tgcgtgctga tttgcccttg taatcttgca
     7981 atctgtttat cgatggcaat gatatgagga tgcgattcag taaaccgttg cctgagttca
     8041 tcgcgtttct gctgcaaaat ggtagtttgt gttttgagtt cgacagagtt tttaagaata
     8101 ttttgcattt cgagattcaa atcgacagac ccttttcggg tgcgatattc atttagtgac
     8161 gttgtcgcgg catctaattg atcttttaga attggcaatt gcttttcaag gaactccagg
     8221 gttttttgtg attccgctga tttttggtcg acattctgtt gaacataaat attggcaatt
     8281 tcgtttaaaa cgctaactgc agcatcaggt agggatgact ccacagttag ttcaagcatg
     8341 ccagtatttt tgcccttttc agcaatcgag atcctttctt tgagttggcg aatcgcttgg
     8401 ttctcggatt ggcgtacgat ggtaaagtgt gtctcaggtc gcgatttcag caatgaaaca
     8461 aaaatagcaa tcggctgcga atagccttcg atttgcttat tcgcaagctt cccgacttct
     8521 ccctccagga tgatttcatc tttatagaat agtttgaaga agccttcctg atctgttttt
     8581 aaaatgaatt cttcatcttc taaaattgac ggaacaatga acgtttctac ttgaatcgtt
     8641 tccccacccc aagcatactg cgttagtcca aagagtggat ttgagacgct tccatcttga
     8701 tttcgatttt gaaactgccg ggcaatggtt tctcctacaa ttggaaaata tctgggtttg
     8761 gctataacat cgagatgtaa gctttttatg gttttaccca aaatcattct cgacttgatt
     8821 aattcgatct cagccataac agccatttta ccttctatca aaccggccaa aggctctaaa
     8881 cccgccatcg aggttgagtt ttctttgact tggagcagac catccgcttt ataaattggc
     8941 ctatctaact gtgttttagc aaaaccaatt aaaactgtga ccgtcattat caatagaatt
     9001 aaccatttac tctcatataa cgtaccgaat agttcaccta aattgataac atcttcatct
     9061 tggttaagag aataatcgaa tgcagtgggt gaacgatcag tgggtaaatt ggacatagta
     9121 ttttgttagt agagaaaaag tgttttttta gcgcatggat atttagtaaa taatagcgtt
     9181 cccattccct tacttgatcg ggttaacgac tagcttgtca ttggttcaat gatcgataga
     9241 ccgttatcgt accgtgcttg gtgctgacgg aaatgctcga aagggttgaa ttcttaacac
     9301 accatcaaac gaattcaata aattcacagt cggttggatt tgagcaatga tttggttcca
     9361 acgaatacct tctgccctat caacataaat gacatcacgc ggttgtagtg ggaaacgatc
     9421 tgctaacagt aatgcgtcgg gtgatttagc atcgaggtga aaaatttctg atttacccac
     9481 tccgcctcta aatacaaata ttctggcagg atcggatgtt tcttgatcga atccacctgc
     9541 gtcgctgagc gcctcggtta atgtcaatcg cgctttattc atgaccaggc ttctagaacg
     9601 tccaataccg gcgccgccaa gcactctaaa attagtttcg cctaatatga aaactttatt
     9661 taatgaacta tccggtacat tgaggatatc tccatgcttt agaagcacat tctgagtgat
     9721 atctccccct tcatataagc ttaacaaatt gatactgtaa gttttatcat ctctcgttaa
     9781 ggtaatgttg cgctgatcgg cttccgactt gacgcctccc gcattattga tcgcttcgag
     9841 cacagttagt ggaatatctt taactagctg aatgcccggt tgagcaactt caccgacgac
     9901 atatacgcgc tggctacggt aatcagcgac acgcacatct aattgaacat tttcaatata
     9961 tcttgataat cgctcggtta actcatcccg aatttcttct accgttcttc ccgcagcatg
    10021 cacaatacct acatagggat aaaaaaaagt tccatcttca ccaacgacat tgcctgcttt
    10081 ctccgctgct cggaactgac ctgcagggat cgtgagttca ggatgatccc agaccgtgat
    10141 gttaaggata tcgcggggcc caacccggta ctgagtataa ggctctgttt ccatgggaac
    10201 gcctttgatg gttcttgaac cgatacggta atcaaaatag tgattggcca cattatcggg
    10261 accaaggctg cgtgtattga aatcacgttc taacgctaag acgagctcag cattaatggt
    10321 ttgaatatca agcgttttta aaattgtctc attattctca gtcactggca ttttaacgct
    10381 cgattgcctg gaaaaaggac tcatgtgttg tcctggtacg attgagcagg cgggaagtgc
    10441 gcacgctgca aggactaaga aaatttttaa atgcatagag ttccctataa aaaaatgtct
    10501 actatgtaaa aatttccgac atcgcaagag agcatgttta agctattctg gcttttttgt
    10561 caaacttgcg ttgaatgtat atacagctga ttacaatttt gttaaccatt gattaatacc
    10621 ctgctcgatg agcaatgctg aatcttcaaa gattgataag tctttctggt aaggatctgc
    10681 tatttcaaat tttccccatt ctcccagccg aaaaactttt cctctagcgc caggctcgtt
    10741 gtcctctata gcctttttat gtgatgtctc cataaccaaa atgaggtcgg ctttgcgaac
    10801 catgacacta ttgagttggc aggcccggtg tgaagaaata tcaatgcctt tttgtattaa
    10861 cacatggcaa gctttctcat ctgctttgtg cccgactagc gcatttagtc ctgcagaagt
    10921 cacaaaatga tttgtttgag ctgattgagc taaatgatgc tttaataacc cttcagccat
    10981 ggggcttctg caaatattac caacgcaaac aaggagtata ttggtaaaca tgagctacct
    11041 ctttttggat ctcgaggcgt taggttcttt tttgtttgta tagccatata gctccataaa
    11101 tcctttaccc gtatgggttc cctctatctt caccgcacct tcccagtaat ggttataagt
    11161 tgttagttga gcatcaaatt cactattttc tactttgctc cgggttttga tatggatatt
    11221 ttttttgggt atcttgatct cccaagcaat ggggtaggtg tatcccgttt tcttgctagt
    11281 ccactgatca cccagctgag aaagcgtaaa gtcactacta agcagagcct cggtaacatt
    11341 attctgtgtg atacttccgg tataaagcag tggtttatcg tacttgtcac gtagttgata
    11401 aatcattaaa tcaattccat tatctagctg tagactaaac caatcccaag acaagaggtt
    11461 ggttgaaaag tcaccccatt gatgatcaaa ccaagataaa ccccgaacag tttcagtagt
    11521 gttgccaatt ttgattgtcc cggtgatatc cattctggtt cgggagtagt aataactatt
    11581 ccctgcttct tctaagaaaa taataccatc ggtgccatga aagacaggag gtatagtatt
    11641 ggttaatttg agattaaagc taaactgggg tgttgaaaca attaattgat cattgccatc
    11701 atctccaacc attttccaat tatctaaaag gaactcaaac ccattttcga tatttgagga
    11761 aaagcccccg cccgtcgttc ttttttgatc ggtatagtgt ttaccggtct ggtgatcact
    11821 aagcgaaacc tgtttgacca aatggcttac aagctcggta accataaagg ttgtgtagtg
    11881 aaagctgaat tgcttgcctg attcagtcca taaatgcccg ttatagtacc accattccat
    11941 ttttgcaaga tgcggcgcat catctttggg cagattaaca gaactactgg tttttgcttt
    12001 gacttcaaac caattatccg cgggttgcag atagaagact tcataggcta accatacccc
    12061 taaccctaac atcatgatta agatcatctt catgaggact gaggcaagcg agtgattgct
    12121 tttgggttta gttttcatat ttaattcgtt tgatttactg cgtattaaaa caaaagattg
    12181 ttgatacttg aggaactgcc cgaatagtag ttcatattat ccaatgtatt ctgtgatcga
    12241 gtgaagtaaa aatatcgcaa tgtgatattg tttgcgatgg agtttaaaaa aaaccattca
    12301 attcataaat ttatacttta cttgtcatat cgagatagat tagaatgcgt taggattttt
    12361 ctaattaatg cgtaagtata agactatatt agatgtaaat tcttgtaaca tttgttgtgt
    12421 aaattaggct ggctagctga aaaataaata aaaaatgggt taattgaatc ctgaatttat
    12481 tggctgcgtt attgataaag atatctaatt ctaataactg ttggaggatg ctcgaatgaa
    12541 tataaagtat cagattaaaa acttactact agcgacttct atcacgatag ggctatcagc
    12601 aacttcagct atggctaacg ttgttggatt tatgggcttg gaattggatg gatgtttttc
    12661 atcgaatgca aacacggcag tatgtggtcc tcatgagaat aatgctgcag tttttgcaac
    12721 gggtactgct gtatttaatg agtcactatt aagtagattt ggcggtactg caggactcgc
    12781 gactcaacct tttagttatg caggatttgg tgaattatta gtaggcacta gcccaggcgc
    12841 accacttgca ccgtttgcag atataacaag aacctgggct gattatacgg cattgtcaag
    12901 tgatcctaat ctcatgcttg gatttgcgat tctgaatcag attgcggcac atccgaatga
    12961 tgcgagcagt tcttttacct tcgacttaac gccgaatcca ggggatgagt tctctatttt
    13021 ttggagtatt acgaacattg ttaccaatgg ttctgtcaca acgggagatt ggaaagcttg
    13081 gtctgatgat gtcttaccat taaatggatt atcgatggcg ctattcggtt cagcattgcc
    13141 atctgatgta aatttcaatc ttaacctaac gttaaatgcg atacctgaac ctgttacgct
    13201 ggctttagtc gggttaggta tattgggaat gacagcagtg ggtcgccgtc gtagtaacac
    13261 cagcaatatt agcttcgctt gatatattga acaggtaaag tcattttgat caaaatgact
    13321 ttactgtggt aggtagtata ttagatagat tctaactatc tagtaggatc gagtagtatg
    13381 actagcgtaa tttaccggat tacgtatcca aacaataaaa tctatatcgg acaagatcgt
    13441 acaaatagca ttaattactt tgggagtgct tcaagcgagc tgatctctca ggatttcact
    13501 aacgaacagc gccagagctt taccatcaca agggatattc tgtggtcttc cgagtgcgcg
    13561 tctcaatctg aagtaactca tgttgaatac gaattaattg aacgatatca cgctaataac
    13621 ccagcagtgg gttataacca gtttccacca tttaaaaaaa caaactatga agtgaaaaaa
    13681 gagagttaaa ttgattctaa agtggcgctg gcgcaacacc cgcccaaata aggataaccg
    13741 caattaatgc gatgccaacc ccgattgtgc ccacaatcat accgccgata atcacaagcc
    13801 cgtccttact gagtagcccg agtgacataa tcattacacc ccatcccggt aaaaaattaa
    13861 tcatgggaat gggtaaggca atcgtgatgg agaaaatgag gacaaatatc ccgatgatgc
    13921 gttcccaggc agagacgctg atataagtga ttcttggatg caagtagctt tcaattttac
    13981 gtaaccacgg cgctgctttt tgaactaatt tttctaatcc tttgcggcta attttcttgt
    14041 cggttagcca tcgaggaagc cagggtgatc gcatgccaca aatcatttgt agcgagaaaa
    14101 tgatcagtgg taatgaaaaa acggttgtaa aacccggagg agctgggatt ggaaggcaga
    14161 ttgggatggc agcaataacc agtaataccc caaatcccct ttcatggagc atattcttaa
    14221 tttcaccgat acttaattta tcgttgacgt tttgtacaac gactaattgt agtaaatcag
    14281 aggtgttgtg ctgcgattgt tgtggtggtt gttcattctc catgcagtca tccagataaa
    14341 gcgcgctatt tacgtaaaaa aacaaccccc ctaacgaaga gggggcttga aatgatgatt
    14401 ttgtggaacc tcaataaatt ttctaacgtt attgggaagc ttgttgtgtg ggttgattct
    14461 gttgttcgtc aatcacgggt tcgtttacgg cttctgcatc agctttgcgg ccagatgggt
    14521 cagtaaataa atcattgagc gattgaatat agtcgtttgc ttcgtcttta acttccgcaa
    14581 gaatttgctg attagaggaa ttccaacccg atggtttgct cagaaacatg ggtctgaatg
    14641 cagaagtaca tttcaagaac gcctgagtat attgcttgca agagttgtga tcatgtccaa
    14701 attcagcagg tagttttttg actaaacttt ttgcgacagc acctcgaatt gaaaaatgga
    14761 tataaccgat tatactgacc ataataccta aaataatcag cgactgggta tcttgggcga
    14821 tgacgttctc agcaaagggc tgtaggattc cccagctttc ggtcatagta agaatgacgc
    14881 cgcatgcaat gaataaaagc gtaaaaagaa taccatcaaa tagaaataca ctttttttcc
    14941 attgatcaag catatccgtt agcttactta ctacttgcgt ttcaatggtt tcagctgtct
    15001 gttctagcat gcctgcgata cggtaagaac gttctacttc gatttgctgc atacgggcat
    15061 tgatatcagc catgtcttct tcccgttttt tctcaaaacg ttccttgacc tgtgcgtcat
    15121 taatagggtt tgccgctttt ggatcataaa tacgatagaa ccgacctgct aacaacccaa
    15181 cctgagcgag tgatcgttgc caagcagaaa caacttcttc aggattatct tccttagcag
    15241 ttacatccat ttgattcagg atatatagga atttattggc atctgcgcgg ttaatagtgc
    15301 ccgatactaa atactccaat gtatctttca tagcgccggg ttccggatgg cgcgcgtcaa
    15361 agaaaaccag tacaaggtct gataaatcga tgatgtgctg tgtcaaacgg agagtcgaag
    15421 tgcgttgact atctgcatca aatcctgggg agtcaattaa aatttttccc cgaatttttt
    15481 cagaagggca agttttaagt tggatataag catcgatacg ttgcggccct gtttctgaaa
    15541 tctcagaaat acttcgactt atctgaaaga atgggaagcg tggatcggca tcaagtgcaa
    15601 caccggggag tgttttaact tcatcttggt cggtgtagca aataacggta aatttatcat
    15661 caactgcttg attacccgtt ctttgtaaat ttaagtttaa ataggaatta atgaaggtgg
    15721 attttccagc agaaaaagtt ccaagcacag cgatcattgg ccaccaagtt acataggtcg
    15781 cataggattc attgtgatcg agtaatccca tgtcttgagc aacccgatcc aatttgcgga
    15841 agctatggac tgccttggcc aagacgggat tgtttggatg ttcatcggct aaatgttttt
    15901 caagactttt gagtcgcttg tggatattgg ttgagctttg tgacataaaa atccttagtc
    15961 ttattggtta aatagttaaa tataatatta acgagtgaat atttttgtta acgtaatcaa
    16021 gctaatgctc gtggcctatt ctagtgtgtc tactatccct ttacaatcaa gcaacacatt
    16081 taaatcggta tcttaacatt tacttcatgc tacgaacgta gctcggctag cagttttacc
    16141 tgcatttgtt ccgcttgacg tagataacct cccccaaata aattgagatg gttcaatata
    16201 tgatagaggt tgtataacgt cttgcggatg ttataacctt tatccagcgg gtaagcttct
    16261 tgatatgcag cataaaattg ggaagggaaa ccgccaaata gttcggtcat ggcaaggtca
    16321 gtttctcgat ccccataata aacggcagga tcataaatga taggttgtcc tgattggtct
    16381 gaggcacaat taccactcca taaatcaccg tggagcaaag caggaacaat cgattgacta
    16441 ggaaaaaata aatcgaggtc tgttagtaat ttttcaccca gttgctgtaa ttttccccca
    16501 tagccttttt gtttggcaag atcaagctgg tatccaaggc gttgatggcg ccagaaatca
    16561 acccagtttg cagtgtgatt atttttttga agcgtagcac caatcgtatt atcacgaaac
    16621 caaccgaatt gttgggaggt aatacgatgc agtgcagcta agttttgacc taatattaga
    16681 taaccgtttt tattgactgg attgagactg atatattcga gtactaacca tgcgtttgcc
    16741 gtgttatatc cataacattt tggttcgggc acacgtatcg tatgggtttc ctggatctct
    16801 gtaagtcctg cagcttctgc ctcaaaggaa gggagaaaag aggctttgtt gagtttcaca
    16861 aaataatcac aatgatcgcc ctgaattcgg taggtttgat taatgcatcc tcctccgata
    16921 gtatcaatcg ccaggatgtt aaagttacga tcaaattgac gaatttgctg agtaatatca
    16981 agccaaggcg ttgttagtgg ttttttggca ttattcatat tatctaagtt aaatcatgcc
    17041 ttgcttgtgc aattgcttgc cgcacttgat cgggagccgt gccgccaaca tggttgcgac
    17101 tttttatgga tccttctagt gtcaaaactg aaaaaatatc ttcttcaatg agtggtgtga
    17161 attgctggag ttcagtaagc gtcagtttac tgagatcgca agatttactt tctgcaaaac
    17221 ggacggcttg cgcaaccatt tcatgggcat cacgaaatgg aatgcctttc ttgactagat
    17281 aatctgcgag atcagtcgcc gttgcatagc cccgcatagc agcattacgc atggcttcat
    17341 gattgacagt aatgccaggg atcatttcgc tatagattct taacgtatct gtcaaagtat
    17401 caatcgcatc gaacaagggt tctttatctt cttgattatc tttattgtag gcgagtggtt
    17461 gggacttcat gagtgtgagt aatgcaatta aatgcccatt gacacgccca gtttttcctc
    17521 gcacaagttc gggtacatca ggattttttt tttgtggcat gatcgatgaa ccagtacaga
    17581 aacggtctgc aagctgaatg aaattaaaga gcggattcat ccaaataata atttcttcag
    17641 ataatcgcga taagtgcatc atgatgagcg ctgcgcaggc acagaattca attgcaaagt
    17701 cacgatctga aacagcatct aatgaattgg agcagacacg atcgaaggcc aattgttttg
    17761 caacaaacat tcgatcgatt gggtagctcg tgccagccaa agcggcagcg cctaacggca
    17821 atcgattgag acgttttcgg caatctgtta accgttctcg gtctcttgcc aacatttcat
    17881 gataggccat taaatgatgg ccaagtgaaa tgggttgagc cacctggagg tgagtaaaac
    17941 ccggcatgat agtatcgatg tggttttcag cgagattcaa taacgctaat tgtacagctt
    18001 gtattaggct ggtaatttca tcgatggatg cgcgaagata taatcgaata tcggttgcaa
    18061 cttgatcgtt acgcgatcgt gcggtatgaa gtttcttgcc tgcatctcca atcaaagcgg
    18121 ttaagcgttt ttcgatattg agatgcacat cttcgagttc aattttccaa tcgaatgtat
    18181 tatcgcgaat ttcctgaaga atggtggtga gcccttgttc aatcgcgacc aaatcggcat
    18241 cactaatgat accgactgaa gccagcattt gggcatgcgc tagcgatcct tggatatcgt
    18301 attctgctaa gcgtttatcg aaattgacag aagcagtgta gcgttgcacc aattcggaaa
    18361 caggttcatt aaatcgtcct gaccaggtat ttgtatgttt tttcacaaaa ataagatctc
    18421 cctacattta acatcatcaa atcatcagat ttgattttaa ttattttaga ttagcaattt
    18481 ctcaaattga atcttaactc gactagcaat ttaggttatg agaattgatg catttacaac
    18541 aagcgacaaa tgattgatta aaaaatgaag aactgtcttt tacttcaggg tggaatagtg
    18601 gataattacc aaatttcata cctgtcacat caacaaggag ccatttatga cagatcagta
    18661 caagcaaaat gatagtaaaa ataccattaa ttggactaat cctgatgcac tgttaaatat
    18721 gttaagcgga aagtttgcag cttttgataa attccaaaaa atgcagttgc cttcatggtc
    18781 cgtatgggta ttcttgggta ttctactgct tgtcatcgct tggagccaaa ttgttgtaag
    18841 tcaggccaat tcaagattag agaaaggaca agctgagata gcaaaccagc ttgagctaga
    18901 gcgctcgacc ttgatcagaa aagcccgtga gtatgctgat gctcaatatc ataaagaaga
    18961 agatcgtttt ggccaggttt tatcatgggc ggtgagaggc gaattgttgc gcaataatct
    19021 cgatcaagtt agccagtatt tgaacgagtt ggtcaaaata aaagatacgg aacgtgctgt
    19081 cttgattggc gatgatggga aattattggt ttcaaccgat aaaaaacttg aagaagcggc
    19141 agcaaaagaa atttatcctg aagagttgct gcaacagcgt aaaattacga ttcagtctga
    19201 tgttaacgga cgtaaattat taatcgttcc gatcatgggg ttgaataaca gacttgcaac
    19261 ggtagtcatt agttacaaaa atcccccgct aaacagtaac taatttactg aataaaattc
    19321 agtgaccctc cgtttgccag atgagcggag gggatctttc tcttcaagtt acgcgtttga
    19381 tctgagctcc cactgaagat agtttttttt caatggattc atacccgcgg tctagatggt
    19441 aaattcgatc aatcgttgtc tcatcgcgcg caactagtcc agcaataaca agactcgctg
    19501 atgcccttaa atcggtagcc attacattag cgccatccaa ttgctgaata cctttgatca
    19561 ctgcagtatt accttccacg gtaatatctg cattgagacg tttcaattct tgcacatgca
    19621 taaatcgatt ctcaaaaatg gtttcagtaa tcaaggctgt tccttctgca atggcattga
    19681 gtgccatgaa ttgggcttgc atatcagtcg ggaaagcagg atgaggagca gttcttaagc
    19741 tgactgattg aggtcgctta ttcataacga gatgaatttg atcgcgatcg aagtcgatga
    19801 gagcaccggc ttctaacaat ttacttgtta cagcgtctaa tgtttccgct cgcgcttttt
    19861 ttagcagaat ttcaccggtg gtagcggtgg cggctgttaa gaaagtacct gtttcaatac
    19921 gatctggcat aacttcatag gaaactccat gtaaggcttc aacaccctcg atcgtaataa
    19981 tatctgtgcc tgcgccagag atttttgcac ccatttgatt caggcaatta gcaagatcga
    20041 taatttcagg ttctcgtgcc gcattttcaa taatggtcaa acctgaagca agtgttgcgg
    20101 ccatcatgag attttcagta cccgtcactg ttacgatatc catgagaatg cgggcacctc
    20161 tgagtttact tgcttgagca cggatataac catgttcgat cgcaatttcc gcccccatcg
    20221 cttgaagacc tttgatgtgt tgatccactg ggcgtaaacc aattgcacaa ccacctggta
    20281 atgaaatatg agcttcacca acccgagcga ctaatggtcc taacacaagg attgcagcac
    20341 gcatcgtttt aaccatttca tagggtgcgg ttggattaga gacatctttt gtagataatt
    20401 cgatcttaga atggtcttta cggcgagtgg ctacgcccat ttgctgaagt aggctcagca
    20461 tagtcacgat atccatcaac tcagggacat tgcttaattc aatggtgtct gtcgtcaaca
    20521 atgaagcgca taacaaaggt aatgcggcat tctttgcacc tgaaatggta atttcaccct
    20581 gcaatgggta accaccctga attaagagtt tttgcataag cgatatttaa ggaagaaaaa
    20641 agatgaggat aagcggttgt tctacagcga cagcgaagtt atgattacaa agtacgatgt
    20701 actcgcaccc atattccaaa actataaact taacataaat ggagcaagaa tggctaaaca
    20761 aatcattcag acagttgatg caccgcaggc gatcggaact tattctcaag cggttcgagt
    20821 taacggcggg gatacgattt atctatcagg gcaaatcggg ttagatcctg taacgatgca
    20881 aatggtcgaa gggatcgatg cgcaaattga acgcgttatt ctcaatttaa aagcagttgc
    20941 gaaagcaagt ggcggaagct tgagtgatat tgtaaaactc aacatctttt tgattgattt
    21001 ggctaatttt gcaaaggtaa atgaagcaat ggctcagcat tttcagcaac cctatccagc
    21061 acgagcagcg attggggtag catcgttacc ccgcggtgct ttggtagaaa tggatggcgt
    21121 aatggttcta tcgaattaat ttatccttac tgagataacg tcttaattaa ttttcaagaa
    21181 tgctttgatt acaattcatc attctatttt ttctcggatt gaacagaaaa cacagcagaa
    21241 actagcaaaa ttaagtattc aaaacgatac ggatcttatt ttacacttgc cccttcgtta
    21301 tgaagatgag acgaatcttt atctgatcaa cgatgtgatc gatgggaatg tttttcaggt
    21361 ggaaggagag gtgattcata atgagattat ttatcgacct cgtaaacaac ttattgttca
    21421 gataagtgat ggcagtggta ctttgctgat tcgttttttt aatttttacc ctaatcaaat
    21481 caaactctat tcagttggag tgcgtattcg tgcattaggt gaagttcgac gtggtttttg
    21541 gggaattgag atggttcatc ccaagtgtcg gatcgttcaa aatgaaacgc cattggcaga
    21601 tacattaacg ccagtttatt ctacaacagc aggtttgtca caacccagta ttcgcaaatg
    21661 gattcaacaa gcacttacac gaatggacca tcaggagttg ttgtgtgaag ctcttcccca
    21721 agaaatcttg tcacgctatc aattattgga attcaaagcg agcctttttg tgttgcataa
    21781 gccattggca ggaattagtg ttacctcact gcaagaaagg aagcatcctg cttggcgcag
    21841 gataaagttt gatgaattat tagcgcaaca gctttctatg cgcctgcatt atcagcagcg
    21901 aaggaataat cttgcacctg tattgacacc aaaacaatcg ttgaccgatc aattgttatg
    21961 cgcattacca ttttgtctga ctgctgcgca agaaaaagtt ttagttgaga tttttcacga
    22021 tctggcagca cagcaaccca tgcaacggct cttgcaaggt gatgtgggta gtggcaaaac
    22081 aattgtttcg gctttggctg cattgcgtgc catcgagaat ggttatcaag tggccataat
    22141 ggcgccaaca gagattctag ccgagcaaca tcacagtcga tttatttctt ggctagcgcc
    22201 actcgatatt gaagtggctt ggctttctgg gagtcaaact aaaaagcagc gggaaagaga
    22261 acttgcccga atttcgacag gaaaagccat gttggcaata ggcactcatg ctttgttcca
    22321 ggatcaagta gcattttcac ggctaggcct agtgatcatt gacgagcagc atcggtttgg
    22381 ggtcgcgcag cgactggcgc taaaacacaa gggaaataag aaagcgttgg ttcctcacca
    22441 attaatgatg agtgcaacac ctataccgcg aaccttggcg atgagttatt acgctgatct
    22501 cgatgtatct actatcgatc aattaccacc gggtcgcgcg ccgattgtca caaaattgat
    22561 tgataatagt agaagagctg aagtcaccca gcgtgtgtat gaggcttgta atacgggaaa
    22621 acaaatctat tgggtatgtc cacttatcga agagtcagag gcgttagagc ttaaaacagc
    22681 cattgaaact tatgaggcgc tcagtcagca cctctcagat atcaaggtag gtttaattca
    22741 tggcagactt aatgcttcag aaaaatcgag tatcatgact gattttagcc aaggtaacat
    22801 ccaattattg gttgcaacca ccgtcattga ggtaggagtc gatgtaccta atgcatcttg
    22861 gatgattatt gagcatgcgg agcggctggg tttgtcccag ttgcaccaac tgcgcggtcg
    22921 tattggccgt ggccaggaag caagtttatg tatattgcta tatcagaaac ccttatccaa
    22981 aatcgcacgg gaacggttgc gtattatttt tgaacataac gatggatttg aaatcgcgca
    23041 gcaagatctc cgattgcggg ggcctgggga gttacttggc acgcgtcaaa gtggtattcc
    23101 tttgttgcgc tttgctaatt tagaggaaga taaggattta ttagaagcgg ctcgcaatgt
    23161 tgctgaacaa ctgctaaatg aaaaccctaa agttgttgaa ttttatctaa cgtggtggtt
    23221 ggcatcaaga aatgtcctgt tacaagcata acttaagtgt aaaatggttt ttctatacgt
    23281 taattgaatc atcgcaatcg gtggtttctt tatactgtat ccatgaatat ctattaatga
    23341 aaagctaaga tgaaaattct ttaccatcga cagtcaatca atttactgct tcgctgggtg
    23401 ttctcactgt tttttttgct aatggttact gcatgtgcat tagggcctga ttatcgtcgt
    23461 cctgcaatcg aagtggccga gcattatcga atacctcatg cggaaggaga atcgattgca
    23521 aaccttcctt ggtgggagct atttcaagat aaagtgcttc agcaattgat tcaacaagca
    23581 ttacaagaaa ataaagacct aaaacgtgta atttcaagta ttgaggagta ccaggcgcgc
    23641 ttatcgatgg caaatatgga ttttgcgccg caactagata cggatatcac tgctccagtt
    23701 atgggcaaaa taggaggatt ttctcgtcct ggattcgcta cacccttcaa ctatttcggg
    23761 atggttaatt taaattggga aatcgatatc tggggaagga ttcgtcgttc gacccaagct
    23821 gcgtttgcgg aattgattgc ccaagaagcg aatcgtcgtg ctatcgtgct atcgttagtg
    23881 ggagcagttg cgcaaagtta tttcgatttg ttgcaactag ataagcaact tgaacttgct
    23941 cagcaaatat tgcattcttg ggaggaatcc gttgctattt ccgatgctca attgcaacac
    24001 ggcttaattt cgaagttaga taacgaccaa tttcaagccg agcgcgcgaa tgcggctgcc
    24061 agagtagcga tattagagcg tgaaatggta cagaaagaaa atgaactgag tcttttgcta
    24121 ggcaagagtc caaattcagt actacgtggt tccgcaatca atgaacaatg gctttctcct
    24181 gaaatccctg ctggcttgcc gtctgaatta ctagatcgcc ggcctgatat tttgcgggat
    24241 gagcaagtac tagcggcagc tactgctaga attggggcaa cgaaagctgc gcgccttcct
    24301 cgaatttctt taacaggttt tttgggggtt gcaagccctt cattgtcgaa tctattgtta
    24361 tcgaatagtc agtttggagt aggggggttg gggctcgcag cgccaatatt gaatgcacgc
    24421 gcattgggct tcgaacaaaa agctgtcgaa gcacaagcaa agcaggcgct tgcacaatat
    24481 gagcaaactg ccttgattgc ttttaaagag gtggaagatg cactggtcgc gattcggacc
    24541 gtcaaagacc aatataaggc aactcaagaa caagttaagg cattacagtc agcgctacaa
    24601 attgcggatc ttcgctatca aagtggacta acaagctatg ttgatgtgct gcttgctaaa
    24661 cgtagcttat ttgatgcaga aacagcgttg gtttcaactc aacggttcca tttagtggca
    24721 cttgttcagc tttataaagc cttagggggt gggtgggcgc cttgaagata cacgctgttg
    24781 tgctgtgcag gtacccaaat tcaatctatg cggctggtat ttaggccgat atttgattat
    24841 cgtatcaatc ttattgttca atcgaggttt tctactaacg aaatcatgat gtatccatta
    24901 atttcatggg tttttcgggg cactcgtcaa attaaattgc aggtaattta tgtcggtttg
    24961 gggctattcg tttgcctcgc gatcggttgt tcagataagc ctgccacaga tcttgctgat
    25021 tcaccgttac cccaagtggc agtgataacg ctaaagactc agtcagtagc tgatgagcct
    25081 gagtttattg ggcaaacaca atcatttcga ccggtagaaa ttcgatcgca agtgagtggg
    25141 attatcaaac agattttttt tacggaaggt agaaatatta aaaaaggaga tcgactttat
    25201 cagattgacc cgattccttt tagagcagct taccaacgag gtcaagccaa tgttgcgcaa
    25261 gcgaaagtta gattaaagca ggctcagcaa gatttagcta gagtgcgtcc tttgttggag
    25321 aagaaagcag ttagcaaaaa aaatattgat gatgcagaag cagaggttca agcagcaaag
    25381 gcgacattgg atgcagcaca gaatgagttg actaaggcaa aatttgacct ggataacacg
    25441 ctgatttatt caccggtgaa tgggagaatc ggtcgcagtc agttttatga agggcgcctt
    25501 atcgaggctc aagaatcctt attaacagcc attgctcaac tggatcccat gtatgtgaat
    25561 gtcaatgttc ccgaaagcta tttattgaaa cggcggcgtg aactggttga gcaaaaagtg
    25621 gcaagccccg aactttttca gctgcgtggg gaaatgactt tttcagatgg cactgtttac
    25681 ccggaagaag gagtacttga ttttgcggat ataagactac gcccagaaac aggtacactg
    25741 caagggcgtt tccgattctc gaatcccgat ggaaaagggg agccaagtgt caggaatttt
    25801 ttatcgggcc aatttgtcaa ggttcgagtc aaaggttata ttcgccacaa tgccatactt
    25861 gttccgcagc gcgctgttca tcaagggccc aaaggatcat tcgtttttat aattaatgaa
    25921 gaagataaag tggaacttcg ttcagtttta gcaagcagct ggcgtgggaa agaatggctc
    25981 atcgagagtg gattaagtgc gggagaaaga gtcgtggtag aaggtttttt tcgtatttta
    26041 cctggagtca aggtgaatgc agttgcttat tctctgatag agaacgaatc tgcttcacgg
    26101 catgattaac gtaatttgta ctaaataact ttcatgaatt ctcatttttt tatcgatcga
    26161 cctatttttg cgtcggtact atcgattatc atcgtagtaa tcgggttagt ttctttgcaa
    26221 acattacccg ttgcacagtt tccgcagata acgcccccga tggtacaaat cgatgcggat
    26281 tatccaggag cgagtgcaga ggtggttgca gaggctgttg ctcgaccaat tgaggtgcaa
    26341 ttgcctggta ttgataattt attgtattac gaatcgacta gcacgaatga tgggcacatg
    26401 atgatgcgcc tcacattcga aattggaacc gatatcgata tcgctcaagt acaagccttg
    26461 aatcgacaac gactagctga gccgcaatta ccagccgaag taattcgaca gggcattact
    26521 gtcaaaaaaa cttcaccaga tcttttagcc gttgttgcgt taagttctac ggatcctcaa
    26581 cacgatactg tttatctatc aaattatgcg ctactcagag ttcttgataa tattaagcga
    26641 ttatctggtg taggtgacgc aattatattt ggtgagcaaa attattcgat gcgaattatc
    26701 cttgatccta ttcggatggc acagctcaat gtaacgccga gcgaaattat cgcggtggtt
    26761 caggaccaga atcgcgattt tccagcggga cggattggtc gtgagcctac tgcacaagga
    26821 acggaactta cgattcctgt cattacgcat ggcagaatga gtgaagtcca tgagtttgaa
    26881 gaaatcatta tccgtgcttt tcctgatgga tcgatgttga aattaaaaga tgtggccaat
    26941 atagaattag gtgcccgttc ttatgatctt caaggccgct ggaatggtaa acccaatacc
    27001 ttccttctaa ccttcttagc cccgggtgct aatgcacttg aaacagtgga acgagtacgt
    27061 gccgagatgg agcggctcgc taaaatgttt ccagctgggg tagcgtatga cattccgtac
    27121 gatacaacca aatttattga agtatcgatc gaggaagtgg ttaaaacttt agctgaagcg
    27181 acattattgg tgctactcgt agttttcctc tttttgcaga attggcgtgc tacgctgatt
    27241 cctgcagtgg ctgttccggt atcattgatt ggtacactga ttggtatgtc ggcactgggt
    27301 ttttcaatca atactcttac gctatttggg atggttcttg caatcggaat cgtggtcgac
    27361 gatgcgattg ttgtcgtgga gaatgtagag cgtcacatga atcaaggagg actttcgtct
    27421 cgagatgcgg cccggaaagc gatggatgaa gtaacgggtc cggtgattgc gattgttttg
    27481 gtattgtcgg ccgttttttt accggtggct tttttaagtg gaattaccgg tgaattgtat
    27541 aagcagtttg ccatcacgat cgcgctatca gtcattcttt ctggttttgt tgctttgacg
    27601 ttgagcccag cgctttgtgc gttggtactg aaaccgagtc ataatgtttc ttccaatctg
    27661 ttttggaagc tgtttaatca gtcatttagt tggttacaaa ctcgttatgt tggagttgta
    27721 tcaattgcat tgaaacgatg ggtgattgca ctcgctattt ttcttgcttt gatggtcata
    27781 atagttggtt tgtttaatag aattcctcaa agttttattc ctgaggaaga tcaaggctat
    27841 tttatcactg tggtgcaatt gcccgatggc gcttcgctaa ctcgaacaac agcagtgctc
    27901 gataaggttg aaagtttttt tcaatcaatt tctgcggttc attcaacgga tacattagcg
    27961 ggccagaatt ttgtgtttgg cacacggggc actaatcagg caaccatgtt tgttcccctg
    28021 aatcactggg atacacgaaa agcagagcag gaccaaattc aaaccttgat taagcgcgct
    28081 tatcaagaat ttgcaacgat tccagaggca ttgattctag cgtttaatgc gcctgcaatt
    28141 agtggattag gctctacagg aggattctcc gcacaaatcc aagatccgag tgggggcgat
    28201 cttgtcgagt ttgctgcaat agcgcaagaa tttgtaacta aagcaactga gcatcccgca
    28261 attgcaatag cgagtaccaa tttcaaaatc agcgcaccgc agttttttat tgaggttgat
    28321 cgggaacgtg caaaatcgtt aggcgtttct atttcagaaa tttttgatac gctgcaggct
    28381 tattttggca gttggtatat caatgatttt attaagtacg gccgtgttta tcgtgtgcaa
    28441 acagaagcga tgccggaata ccgttcgaat cccgataatc ttaatcatat tcatgtgcgt
    28501 gcccaatccg gttcatcaca aacgatgatc cccttgagtt ctgtcgtaac aactcaattt
    28561 aacagtgggc ctgatcctgt tacccatttt aacggattta attccgcaat ggtgcttgga
    28621 agcgcggcac caggctatag ttcagggcaa gcgttggatg ccttacaagc cgtagcggat
    28681 gaaattctac tccccaaagg gtatgccctc gattggagtg gcatatcgtt gcaagaacgt
    28741 aaagcaggcg ggcaatcgct attagtattt gcttttgcgc tattgatggt tttcttagta
    28801 ttagccgcat tgtatgaaag ttggtcgatt ccatttgccg tgatttttgc tattccacta
    28861 ggaattttag gggcattatt ggcaatttgg ttgagggatt taagtaacga tatttatttt
    28921 cagattggat tagtcacctt gattggatta gcagcaaaaa atgcaatatt aatcgttgaa
    28981 tttgccaatc agaattatcg agcgggacaa ccactgattg atgcaacact ggaagcagca
    29041 aaactacgtt tccgacccat tatcatgaca tcaatggctt ttattctagg tgtttttcca
    29101 ttggtaattg catcaggggc tggtgctgca agccgtcact cgatcggaac gggtgttttt
    29161 ggaggaatgt tggcagctac ttttttggcc atcttttttg tacccatttt tttcgtcatc
    29221 gtaaatagaa ttgcacaccg agtgcgtttg aggaatacgc g
//
LOCUS       DAOQKQ010000005        29052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_644_length_29052_cov_5.353761, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000005 DAOQKQ010000000
VERSION     DAOQKQ010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 29052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29052
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_644_length_29052_cov_5.353761"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1837
                     /locus_tag="PKZ49_00605"
     CDS             <1..1837
                     /locus_tag="PKZ49_00605"
                     /inference="COORDINATES: protein motif:HMM:NF024298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HND35088.1"
                     /translation="VTTDKIWNIGAAWFAITQVQDKTYVLALNNEGKCYFWDPSETWP
                     SEPFLTLSDPNIAALAISNDSLQFAIADHNGEIQVWHRSTKSRVKTFKSLQSVFSIGF
                     SPNGQQLAIGSKGVIQLWDLASGMMIHEMANSQDGAIRAIAFSSDGLEIVSGGEDRRL
                     TVWNSQTGRAVGEPIRGHSNTILSVAFSADGTQIVSSGEDKLLRLWDRQTGKQRIDSL
                     RGHEGIITSVAFIENAQQIVSGSRDGTIRLWSTRDHPIIDTPLLGHEDMVTSVAYSPD
                     GKKIVSGSDDKTLRLWNAWTAQPLDEPLQGHEGTINGVVYSPDGARIVSGASDGTLRF
                     WDAQTGLPIGEPLKGHLEGVETVAYSPDGKLVASGSKDKTIRLWNAYTGKQEGEPLSG
                     HEEMVRSVAFSPDGKVIVSGSYDHTLQRWEVATGNKLGSPMEGHTGHVVSVAVSPDGQ
                     YIASGSKDKTIRLWDAQTGESINEPIQGHEARINSVAFSPDNQRIISGSHDMTLRLWD
                     VKTGLPVGDPLSGHLGAVTSAVFSPDGSHILSGSADKTLRLWMANTDWVDELCNKLTR
                     NMSHAEWQEWVSPNIKYRKQCPNLPIYESNTLIGESVKMLSEKGG"
     gene            2226..2645
                     /locus_tag="PKZ49_00610"
     CDS             2226..2645
                     /locus_tag="PKZ49_00610"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HND35089.1"
                     /translation="MFRMIFRRIYVAIDQSEFSGQALIAAMKIAQTSKVLLCPGYCIT
                     DDTLSSQETALNFLTGIKSQISDAVEVEIKIAAADVQYGLNGISTAIAQSAHNWQSDL
                     LVVGTPNREGAKYFYLSAVAGELISKVNCSVMLIRIN"
     gene            2885..3337
                     /locus_tag="PKZ49_00615"
     CDS             2885..3337
                     /locus_tag="PKZ49_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HND35090.1"
                     /translation="MTQYFTKSAARNIFYGGSVFFLLIFIALTIHSHFYAISTATDES
                     TLTESVIRGKHIWERHSCINCHSLIGEGAYFAPELGNVWIRYGGQQSPEGARTGLKAW
                     MRAQPTRIEGRRQMPQFNLSEQELDDLIDFLEWTSRINTLGWPPSISG"
     gene            3395..4738
                     /locus_tag="PKZ49_00620"
     CDS             3395..4738
                     /locus_tag="PKZ49_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HND35091.1"
                     /translation="MKYQTQALAYPYFVAALVLFLVQILAGIMAGSIYVFPNLLSEIT
                     PFHILRMIHTNALLVWLLLGYFGASYFLIPEESEREIHSSRLAWLQLGFFVFAGLAAV
                     ASYLSGVHEGREFLEQPLWIKILMVVAFLIFIYNVTMTVLQGRKTVVSIVLLLGLWGA
                     AIFFMFAFYNPDNLSVDKLYWWWVVHVWVEGVWELIMSAMLAFLLIKMTGVDREIIEK
                     WLYVIVGFSLFSGLLGTGHHYYWIGTPEYWHWIGIVFSFLEVVPFFAMIVWSFYLVRK
                     SGRNHPNKAALFWSLGCPIMAFIGAGLLGLMHSFPSINFYTHGTQLTAAHGHLAFYGA
                     YVMLNLAFFTYAMPILRNSQPFNQNLNILSFWLMNAAMIGITFALLFAGILQTHLQRV
                     MNLGYMQVQSQLELFYWLRLGSGVFFIVGALIFIYSVLGPNREAFVAGKFQHYNV"
     gene            4738..5571
                     /locus_tag="PKZ49_00625"
     CDS             4738..5571
                     /locus_tag="PKZ49_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HND35092.1"
                     /translation="MAHDSVFAAAKGPVSAQSATEIPFYHETGNECALFAAAYREGLP
                     LLLKGPTGCGKTRFVAHMAARLGRPLHTVSCHDDLTAADLTGRYLLQGGETTWLDGPL
                     TRAVREGGICYLDEIVEARKDVTVVLHPLTDDRRILPLERTGEFLQAPDDFMLVVSYN
                     PGYQNILKSLKPSTRQRFVTINFDFPPPIIETRIVATESGLAEERCRLLVDLAQRIRV
                     LKNIDLEEVVSTRLLIYCAILLKNGFDPYQAAQATLIEPLCEDDDVKQGLLELVRATF
                     G"
     gene            5575..7551
                     /locus_tag="PKZ49_00630"
     CDS             5575..7551
                     /locus_tag="PKZ49_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HND35093.1"
                     /translation="MNWLNFTELEEQAGRFWHRIVGSAESYPNYQQAAITLDQVRPAL
                     GIFFRGLGGASGVAIQSGSPRSSGHRLTLRQRLGLMQESLNDINCNTQRILLPASLAY
                     FPDSNLNRLHYFWLAAFFATAREVLYQLGSTKTAQQTGEACIDRLQNDLAFLHQAYWI
                     SSTVCRLNPGLLVIYQQLCSAVLEQRPHRRLPNQERVIEAVIRRLLGQSDSSDEVDAC
                     WLSIQQTTPDFSSWQAGKNYQPFLLVPLWGNIDINQAIDAAKLDGSNDSIGNSGEACN
                     SQHKKTAHRNNQDQSRRDDPLLLNRFEKLLSWSEMVNVNRPVEDDDEQSAKAAEQAIE
                     TITLSPHEEKAVTQLKFDLDLAPDDMNPEMLIGEFTYPEWDFKGMQYHPRQCRVIFQR
                     AQSSCERWALDQQTQKRLRKIRRQFETLLPKRELLRGQYNGADLDMDALVRSHCDFLA
                     TGNYSESIYRNTRKQSRDLSVAILIDVSLSTDSWIDGYRILDIGKEALITLATGLTAC
                     RDSFAIYTFTSRKKDYICVTEVKKFEESFNSPVLQRIAALQPGYYTRMGAALRHVQQL
                     LSQRQERHRLLLMLTDGKPNDLDYYEGRYGIEDTRQAILEARRSGLSVFGITIDNEAQ
                     DYFPFLFGRGGYTIISRPDRLSHLLPAIYQQLIN"
     gene            complement(7709..8545)
                     /locus_tag="PKZ49_00635"
     CDS             complement(7709..8545)
                     /locus_tag="PKZ49_00635"
                     /inference="COORDINATES: protein motif:HMM:NF022673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3025 domain-containing protein"
                     /protein_id="HND35094.1"
                     /translation="MKREHIDQWKHHFLDCSPMFEPLRQAGQLLNQLEQWPTPDQLNH
                     LLSVQKSNIRTNTHHPLRFVTQTTPTSLFEQQYEPRTYLCGELQTRAQNWHDLFNALV
                     WMTFPQSKAQLNALHYHTLKIAEKQHRSARGRLRDAITLLDESGILVTSSHEDLTYLL
                     KNFEWKKLFWENRSDLLQHMKFFLFGHSLYEKALSPYIGMTGKGLIFHIEPSFYQQDL
                     YSQIVSLDNKLANLLSTKSITSTDLTPIPLLGYPDWSPNNSNATYYDNSDYFRPRSNK
                     QP"
     gene            complement(8542..9807)
                     /locus_tag="PKZ49_00640"
     CDS             complement(8542..9807)
                     /locus_tag="PKZ49_00640"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HND35095.1"
                     /translation="MHDTEQILQTGESSELVELGTVPHFTPIQERPIIAVLAGIHFIH
                     IMDFMMLLPLGPQLMKLFELTPQEFSLLISIYAFSAGAFSFFASFIIDHYNRKSFLIF
                     LCGGIGLATLLCALSFEYYLLLLARMVAGGFSGVLSATIFTIVADVTPAHRRGDATGT
                     VMSAFSAVAVIGMPLGILLVNLFSWRAPFLFLTLISLVMIVLAIWLLPSLHGHLKHRQ
                     HRHPAQHLRAVFYNRNHLYAFALIAMLMFAGFSVSPFITTYMITNIGMSEVDIPYLYF
                     CGGLATFYTARLFGKLSDQHGKHKIFSIAAALSVFPILMITHLSTASVLLAIAVSTLF
                     MMLVSGRFVPAMALVTASVTPRLRGSFLSFNISIQQLSAGIASLFAGSIMSTTPTGSI
                     MHYDTVGVIAAIATVLSILLAFQVHSLPE"
     gene            9899..10720
                     /locus_tag="PKZ49_00645"
     CDS             9899..10720
                     /locus_tag="PKZ49_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HND35096.1"
                     /translation="MCQKCMVTSDHCNSNYPASIVQDKHKDPTKREFIKRTSAVLMSW
                     GLLATGLVNASESKTVNEKKRIIPKPENVLTPDQALERLMQGNERYVSGNSKPLDFHD
                     VKTSLVKGQNPYATILGCSDSRVSPEHCFDEALGDLFVARGAGNYLTNDNLATIEYSV
                     AVLSTPLIMVLGHESCGAVKAAIDAVDRHRDFPGHIQLLASAIAPAVRTVSEIAKVSD
                     TTEDRLIHVTKQNVIMTVERLREKTPILDYYHDRKKLKIVGGIYHLDTGRVELIA"
     gene            complement(10774..11064)
                     /locus_tag="PKZ49_00650"
     CDS             complement(10774..11064)
                     /locus_tag="PKZ49_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35097.1"
                     /translation="MRRIILLMFMVLLLCGFRAPNGALISKGDLVDKLIINLGQPLAR
                     SPFVTQRGLYLPYHSGSRGYSVREVWIYKVEQYTYRFIIDNGVIVHEDWTRF"
     gene            complement(11193..12113)
                     /locus_tag="PKZ49_00655"
     CDS             complement(11193..12113)
                     /locus_tag="PKZ49_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HND35098.1"
                     /translation="MLQKLTLLFPLWMLISATFSLFNPEWFTPLNQGSTVVLILAFVM
                     LCMGLTLTIDDFRRIGRMPKAVAAGFLGQYTIMPVLAWGIAMLLNLPIHFAVGLILVG
                     CCPGGTASNLVSYIAKADVALSVVMTVCSTLAAIILTPLLTQLFAGTLVPVDTWMLFK
                     QTLQVVILPIALGITLNHWLPSTVQRIMPLAPLLAVIGVCIICATVFAGQAEGIRHYI
                     GELILATALLHAGGFFLGYTLARYSNHDKQSMRTISIEVGMQNSGLAIVLSKQAFPLL
                     PLAPVVGAISVLMHSLLGSLLAVIWRSQKT"
     gene            complement(12132..12848)
                     /locus_tag="PKZ49_00660"
     CDS             complement(12132..12848)
                     /locus_tag="PKZ49_00660"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HND35099.1"
                     /translation="MSTNQPTTIFTQSWSLYDLITAYNYMFHQEIYEGVQKLLGLRQD
                     VTRYRLMDLGCGNARFMAPCLLKYPPSYYEGVDLSETALTEAKTYLAPLTCPVKLTHN
                     DLLTSIESTQDQWDIIFSGFAVHHLTPEDKTRFFNAVGRSLSKQGWLILVDVVREEHQ
                     DRETYLNHYLQFMRETWTKVPPEQLEEACTHVHDHDYPETFSTLQNMAIAAGLHTQRI
                     ISRYGQHYTLLFSPVNLPKI"
     gene            12993..13367
                     /locus_tag="PKZ49_00665"
     CDS             12993..13367
                     /locus_tag="PKZ49_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HND35100.1"
                     /translation="MNSAPMEASNTAPIEFKPIANEAFAVMIVSEQGLIYDCSEKGAS
                     LLGWMQHDLIGHSVAEVIPKLASITLLKQGRANAYLRFLSRIGHHFELIGKNDMRTTG
                     EIFFSDVQQEGVHYLLLTIQLN"
     gene            13747..14910
                     /gene="carA"
                     /locus_tag="PKZ49_00670"
     CDS             13747..14910
                     /gene="carA"
                     /locus_tag="PKZ49_00670"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HND35101.1"
                     /translation="MLQQPRAILVLADGTVFQGISIGIKGITVGEVAFNTAMTGYQEI
                     LTDPSYCQQIITLTYPHIGNTGSNKEDIESAGSVKVFAAGLVIRDLSLTASNWRKTES
                     LPTFLENHNVVAIAGIDTRKLTRILREKGAQAGCIMAGDINIEAALQRAQTFPGLVGM
                     DLAKVVSCQHAYQWTESDWRLGYGYASQPTIDYRIAALDFGVKQTILRRLASRNCSVT
                     VYPAQTPVEEILLSNPDGVFLSNGPGDPEPCDYAIDTVRRLIDMKIPIFGICLGHQIL
                     ALAAGAKTMKMKFGHHGANHPVLDIDSGKVIITSQNHGFAVDMNQLPTNIRVTHLSLF
                     DKSLQGFELINSPAFCFQGHPEASPGPHDASYLFDRFIDRIKQYKKQQSHVAK"
     gene            14944..18153
                     /gene="carB"
                     /locus_tag="PKZ49_00675"
     CDS             14944..18153
                     /gene="carB"
                     /locus_tag="PKZ49_00675"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HND35102.1"
                     /translation="MPKRTDIKSILVIGAGPIIIGQACEFDYSGVQACTALREEGYRI
                     VLVNSNPATIMTDPEMADATYIEPITWPMLEKIIDIERPDALLPTMGGQTALNCALDL
                     VKYGVLDKYGVELIGASREAIDKAEDREKFKQAMAHIGLSSARSAIAHSMEEALQVQA
                     MIGYPVIIRPSFTLGGTGGGIAYNRQEFIEICERGLDASPTRELLIEESVIGWKEFEM
                     EVIRDKQDNCIIICSIENLDPMGIHTGDSITVAPAQTLTDKEYQIMRNASIAVLREIG
                     VETGGSNVQFAINPADGRLLVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLNE
                     LSNDITGGVTPASFEPTIDYVVTKIPRFAFEKFPQANDRLTTQMKSVGEVMAIGRTFQ
                     ESFQKALRGLEIGVDGLDEKSSDLDLIAAELGHPGPERIWYVADAFRHGMAFDEIYQL
                     THIDPWFLHQIENLIQQEQSLTDSTVDSIDTDLLRQLKRSGFSDRRLAKLLNSSELAI
                     RTRRLNEKIYPVYKRVDTCAAEFSTDTAYLYSTYEEECEANPSTRKKIMVLGGGPNRI
                     GQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDISDRLYFEPLTLEDVLNIV
                     AVEKPNGVIVQYGGQTPLKLARDLERFGVPIIGTSPDMIDCAEDRERFQDLLNRYNLK
                     QPMNRVARNVEMALAAATEIGYPLVVRPSYVLGGRAMEIVHEQKDLERYMQEAVKVTN
                     DSPVLLDHYLNNAIEVDVDAIFDGKTILIGGIMEHIEEAGVHSGDSACSIPPFSLSTE
                     LQQELRRQTEVMARALNVIGLMNVQFAIQNDIVYVLEVNPRASRTVPFVSKATGLQLA
                     KISARCMVKKSLYDQAIFKDIEPTFFSVKEAVFPFSKFSGVDTILGPEMKSTGEVMGV
                     GNTFAEAFVKSQLATGIKLPISGKIFISVRASDKLEAVTLAKKFIELGFTIYATQGTA
                     EVMIASGLNVHKVNKVAEGRPHIVDMIKNGEICLIINTVANKRRAIQDSYSIRHAALL
                     AKITYYTTMAGARAAYMGISNMRELHAYSMQMLHHQTIQ"
     gene            18190..18666
                     /gene="greA"
                     /locus_tag="PKZ49_00680"
     CDS             18190..18666
                     /gene="greA"
                     /locus_tag="PKZ49_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HND35103.1"
                     /translation="MSPIPLTVAGAEKLRAELHEMKTVQRPAVIAAIAEARSHGDLSE
                     NAEYDAAKERQGFIEGRIAELESKLANAQIINPALIDADGACVFGATVELKDFGGNVT
                     VIYQIVGDDEANLKEKKISISSPIARALIGKYAGDIVEVVAPAGVREYEILNIQYI"
     gene            18872..19495
                     /locus_tag="PKZ49_00685"
     CDS             18872..19495
                     /locus_tag="PKZ49_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HND35104.1"
                     /translation="MKSNKVNRAWIHEHVNDFYVKQAQKAGYRSRAAYKLIEIAERDQ
                     LFSPGMIVVDLGAAPGSWSQVCRERIGKSGKIIALDLLNMLPLDGVAFIQGDFSEEKV
                     CEMIENQLAGDLADLILSDMSPNLAGIGMSDQARSINLAELALAFANNKLKPGGSFLV
                     KIFQGSYVDQYIRDMRFHFNKVMIRKPKASRSKSKELYLLGLGKIIC"
     gene            19640..21541
                     /gene="ftsH"
                     /locus_tag="PKZ49_00690"
     CDS             19640..21541
                     /gene="ftsH"
                     /locus_tag="PKZ49_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HND35105.1"
                     /translation="MNNLIKNMAIWLVIAIVLMTVFNQFTVRQSAQSPIEYSQFINEM
                     NQGRIAKVTIDGRTLKGTKSDGRQFITFAPSDPWLVSDLLRAGVIVEAKPEEEPSILM
                     SIFVSWFPMLLLIAVWIFFMRQMQGGGRSGGAFSFGKSKARMLDKNSNHVTFADVAGC
                     EEAKEEVAELVEFLRDPTKFQKLGGRIPRGVLMVGNPGTGKTLLARAIAGEAKVPFFS
                     ISGSDFVEMFVGVGASRVRDMFEQAKKHAPCIIFIDEIDAVGRQRGAGLGGGNDEREQ
                     TLNQLLVEMDGFEGNMGVIVIAATNRPDVLDPALLRPGRFDRQVTVPLPDIRGREQIL
                     QVHMRKVPISPDVHADVLARGTPGMSGADLANLVNEAALFAARSNKRLVDMDDFERAK
                     DKILMGAERRSVVMPENERRNTAYHESGHAVVAYLLPKTDPVHKVSIIPRGRALGVTM
                     QLPTEDRFSMDRDQILQTISVMFGGRIAEEIFMNQMTTGASNDFERATETTRRMVTQW
                     GMSDVLGPMVYGENEGEVFLGRSVTTHKNVSEATMQKVDAEIRRIIDEQYALARKLIE
                     ENRDKIEAMTKALLEWETIDSEQIKDIMENRPPRPPQPPQSKSSAAPKEDNKTVSSTA
                     ANEGSAQEV"
     gene            21724..22494
                     /gene="folP"
                     /locus_tag="PKZ49_00695"
     CDS             21724..22494
                     /gene="folP"
                     /locus_tag="PKZ49_00695"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HND35106.1"
                     /translation="MGIINVTPDSFSDQGRYAASEKAIEHAIKLIADGADLLDIGGES
                     SRPGSACVSLDEELNRVIPVIEALKSAAIPISVDTTKPEVMQAAVQAGVSMINDINAL
                     RTEGALKIIAESGVMVCLMHMQGLPKTMQDNPLYADVVSEVREFLQQRIQIAQDWGIS
                     NDQLVIDPGFGFGKTLQHNLELFRSLDQFTTLNIPVMVGVSRKSMLGTITGNDVNHRT
                     YASIAAAVLAAEKGAKILRVHDVKATKEAILVFNALNR"
     gene            22553..23932
                     /gene="glmM"
                     /locus_tag="PKZ49_00700"
     CDS             22553..23932
                     /gene="glmM"
                     /locus_tag="PKZ49_00700"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HND35107.1"
                     /translation="MKKKYFGTDGIRGRVGEFPITPEIILHLGYSAGKVLLESDWHLK
                     KGEKPVVLIGKDTRVSGYLLESALEAGFSAAGVDVLLSGPLPTPAIAYLIRALRLQAG
                     IVISASHNPFDDNGVKFFSSEGTKLPDEMELKIEMGLEAPIRTMPSVKLGKVQRLKDA
                     TGRYIEFCKSAFPYHLDLRGLRIVIDCANGATYHIAGHVLHELGADVVTICANPDGFN
                     INHQCGATHCATLQSAVKEHAADLGIALDGDGDRVIMVDNDGVIYDGDLLIYIIAKHR
                     YSKGMLRGGVVGTSMTNLAIEKAMMLLNIPFERSNVGDRYVLELLNKNGWQLGGESSG
                     HIVCLDKHTTGDGIISALQVLYALRDSKLNLREATGDIKLFPQRLINVRVAKGLNYLS
                     NPSIQAIVQQAENDLNSEGRVLLRASGTEPLIRVMVEGSNQQKVNSWAEKIANSVQEI
                     AQQQESKQS"
     gene            24099..25094
                     /locus_tag="PKZ49_00705"
     CDS             24099..25094
                     /locus_tag="PKZ49_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HND35108.1"
                     /translation="MLNKRSFISVIGGGLLLILSSQLSARAIVMIDGSSTVYPITEAV
                     AEDFQAMKKGEIRVTVGISGTGGGFKKFCRAEIDIANASRPIQKAEMETCKAAGVEYI
                     ELPVAFDALTIAINPKNTWSKTITVAELKAMWEPSAQGKITKWNQINPAWPDEKIKLY
                     GAGADSGTFDYFTEAIVGKAKSSRGDFTASEDDNVLVQGIASDKFSLGFFGFAYYIEN
                     QKKVSAAAVDNGKGGVTPSAEAVENGSYQPLSRPIFIYVNAKALDKPEVREFINFYMN
                     NAPALVKEVKFFPLPAEVYQLNLDHLEKRKIGTVFGGESKIDLRIEELLKLEKQM"
     gene            25219..26607
                     /gene="cysG"
                     /locus_tag="PKZ49_00710"
     CDS             25219..26607
                     /gene="cysG"
                     /locus_tag="PKZ49_00710"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041357033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HND35109.1"
                     /translation="MDFFPIFLNIRDRKCLVVGGGEIAARKVELLLKANALVTVISPT
                     LGETLEAFSSQSQISYIRDIFHPKYLTNYTLVISATNNHQVNQQVWEEAIKANIPVNV
                     VDNLSLCSFIMPAIIDRSPVVIAISSSGKSPVLVRMLRTRLETLIPGAYGKLAQYLGE
                     FRDLVKQHFILPEQRRFFWEKLLQSPFVEMVLSGKDQAAQQYLIKSIENDPKDLPQGE
                     VYLVGAGPGNPDLLTLRALQLMQQADVIVYDRLVSKSIMDLTRRDATRIYAGKERNNH
                     TLSQESINELLVRLAKDGKRVLRLKGGDPFIFGRGGEEIETLSEHRIPFQVVPGITAA
                     SGVASYAGIPLTHRDYAQSCTFVAGHLKHNSLDLDWNTLARPQQTIVVYMGLLGLPIL
                     CEQLIAHGLPATTPAAIVQQATTNNQRVVIGSLDTLPSLAMNAHIVPPTLIIIGEVVK
                     LHQKLTWYHYDY"
     gene            26844..28280
                     /locus_tag="PKZ49_00715"
     CDS             26844..28280
                     /locus_tag="PKZ49_00715"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HND35110.1"
                     /translation="MTIREQAIAGIKWTAGGKLAAQAITWGITLIVMRLLHPEDYGLL
                     AIASVFLAFMMMLSEAGLAPALVQKQELSKIALQQVFAIIIVINLALLALLNLLAPII
                     ANFFNDERLILILNVMSLQFLTVIAITIPQVLLYRELKFKYLSIIDFIAVITGSLFTL
                     ALAILDYGVWALIYGNLITAVCKVIAFNILTPTYLLPKFSLIGMRGFLSFGGNITLSR
                     LLWFFFTQIDVIIVGKLLGKELLGYYSIAMHLASLPVQRISSIIGQVAFPVFSKVQDD
                     QQQFRNFVLKSIRILSLIAFPVLWGISSVANEMVLLFLGEKWFHAILPLQLLSLMMPF
                     RMIANFLPSATDALGHPEIGVKNVFLAAIVMPIAILIGVQWGIVGVAIAWVTVYPIIL
                     FINYHRSLSVIGLKLKDLFYVLFPVLISSVGMYLMVWGVDLLLENQLNKLLQLTIMIL
                     SGGLAYYGLTIKFNNAGYRDFIQLFKKN"
     gene            28382..>29052
                     /locus_tag="PKZ49_00720"
     CDS             28382..>29052
                     /locus_tag="PKZ49_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35111.1"
                     /translation="MAIINKKSGLFLIRILVLIIVLVWVWMPEFLLWHVNLKLPTAEI
                     INESRSLPSTSTLIELGQMDLKPPSNPLDDSQVIILAEKALNGILSVPGFDDIAASPI
                     FNESDLNQGSALQQLIFASLITVDYLLEAYRITHQDKFYVAALKSIQAFCNFESSRFL
                     DIGFLWNAHAIAARVPVLIKFWLYYRDREDFEVILAQQLFKLIVRSGLLLAKPEHYSW
                     RTGHG"
BASE COUNT         8564 a         5354 c         6462 g         8672 t
ORIGIN      
        1 agtaacgaca gataaaatat ggaatattgg tgcggcatgg tttgcaatca cccaggtgca
       61 ggataaaacc tatgttcttg cactgaacaa tgaaggtaag tgttatttct gggatccctc
      121 agaaacatgg ccgagtgaac cctttttgac attaagtgat cccaatatcg cggcattggc
      181 tatctccaat gatagtcttc aatttgcgat cgctgaccat aatggggaga ttcaagtttg
      241 gcatcgatcc actaaatccc gagtaaaaac ttttaaaagc ctgcaatctg tttttagcat
      301 cggcttttct cctaatggcc agcaactcgc aatcggtagt aaaggggtga ttcaattgtg
      361 ggatttggca tcaggaatga tgatccatga aatggctaac tcgcaagatg gggcgattcg
      421 cgcgatcgct ttctcaagtg atggacttga aatcgtttcc gggggagaag ataggcgctt
      481 aaccgtttgg aatagccaaa ctggtcgtgc ggttggtgag ccgattcgag ggcactcaaa
      541 cactattctt agtgttgcgt tttctgctga tggaactcag attgtatcga gtggtgaaga
      601 taaactgctg cgattatggg atcgccagac tggtaaacag cgaatcgact ccttacgtgg
      661 ccatgaagga ataattacca gtgttgcttt cattgaaaat gcacaacaaa tcgtttctgg
      721 tagtcgagat ggcaccattc gtttatggtc gactcgtgat caccccatca tcgatacccc
      781 tttattggga catgaagata tggttacgag tgtagcctat agcccagatg gtaagaagat
      841 tgtgtcgggt agcgatgata aaacactgcg attatggaat gcttggacag cacaaccgct
      901 tgatgaacca ctacaaggcc atgaaggaac aatcaatggt gtggtctata gtcccgatgg
      961 ggcacgtatt gtttctggtg ccagtgatgg tacgttacga ttctgggatg cgcaaactgg
     1021 gttaccgatt ggtgaaccct taaaaggcca tctcgaaggt gtagaaacag ttgcgtatag
     1081 tccggatggt aaactcgttg catctgggag caaagataaa accatccgat tatggaatgc
     1141 ttatacagga aaacaggaag gggaaccgtt atccgggcat gaagaaatgg tacgtagcgt
     1201 agcttttagt cccgatggaa aagtcattgt atccggtagc tatgatcata ccttacagcg
     1261 ctgggaagtc gcgacaggta ataaattggg tagtccgatg gaaggccata ctgggcacgt
     1321 cgttagtgtg gcagttagtc ccgatggtca atatattgct tcgggtagca aggataaaac
     1381 gattcggtta tgggatgcac agacgggtga gtcgattaat gaaccgattc aaggccatga
     1441 agcacgtatc aatagtgttg cttttagccc cgataatcaa cgtattattt ccggcagtca
     1501 cgatatgacg ttgcgccttt gggatgtgaa aacgggtttg ccggttggtg atcccttatc
     1561 aggacatctt ggtgcggtta cgagcgctgt gtttagccca gatggttccc acattttatc
     1621 gggaagtgcg gacaaaacgt tgcgcctgtg gatggctaac acggattggg tagatgagct
     1681 ttgtaataaa ttgacgcgta atatgagtca tgcggagtgg caagagtggg tttcaccgaa
     1741 tattaagtat cggaaacaat gtcccaattt accgatttat gaatccaata ccttgattgg
     1801 ggaatcggtc aaaatgcttt ctgaaaaagg ggggtagaac acgccaaatc ttgcgtttct
     1861 tgtaattcga gtttttgcta gtaatcttca tcagataatt aaaaacgata gtattaatgg
     1921 gaaaattatt attaccgact atctgcatat acttcttctc aactatatct aatgaaaagt
     1981 attacctctt tctaaatata ttcattatat aaaattaata ctttgcccaa tatacggaac
     2041 actttctata gcagtagact attctcgtaa ttcaagttta agcatgtttt tctgaattgc
     2101 tagcttgtat ttatttaact ggagcaacaa aagagactag ttcatggcag caaagataag
     2161 taaaagtact attggttacg cgccacatca ttctgaacat aattaactat tagaaataag
     2221 taattatgtt cagaatgata ttcagaagaa tctatgttgc gattgatcaa agtgaatttt
     2281 cggggcaagc attaatagct gctatgaaaa ttgctcaaac ttccaaagtg ttgctatgcc
     2341 cggggtattg catcactgat gacacgctat ctagccaaga aacagccctc aatttcttaa
     2401 caggcattaa atctcaaatc agtgatgcgg tcgaggttga aataaaaatt gcggctgctg
     2461 atgtgcaata tggtttgaac ggaatcagta ctgctattgc tcaatctgca cataattggc
     2521 aatcggattt attagtggtt ggtactccaa atcgagaagg agctaaatat ttctatctga
     2581 gcgctgtagc aggagagtta attagtaaag tcaattgttc agtaatgctg ataagaatca
     2641 attgattctc aaatacttta ttaaatgtga agtaaagcag tatgagtagc tcctctgata
     2701 ccgcttaatt tttgagagcc tagctgtatt gaatcaaatt atttcaacaa attaatttgt
     2761 cctccataaa tttaaattat aatcgattgg catcttgaaa aaatatacat aaccatatga
     2821 ttattattgt taaattgatg ttttattaga ataatattgg ctattaatta aggaacaaat
     2881 ctatatgaca cagtatttca ctaaatctgc tgcacgaaat atcttttacg gcggatcagt
     2941 tttttttcta ctgatattta tagcgttgac gattcatagc cacttttatg cgatcagtac
     3001 tgccacggat gaatcaacat taacggaatc ggtcattcgt ggaaagcata tctgggagcg
     3061 tcattcctgt atcaactgtc attcactaat cggtgaaggg gcatatttcg cacctgaact
     3121 tgggaatgtt tggattcgct acggtgggca gcaaagtcct gaaggcgcaa ggaccgggct
     3181 caaagcatgg atgcgtgcac aacctactcg aatcgaaggt cgacggcaga tgccccagtt
     3241 taatttgtca gaacaggagc tcgacgatct tatcgatttt ctggaatgga ctagtcgcat
     3301 caatacactt ggttggcccc ctagcatttc tggatgattc cattgggacc gtttatatgc
     3361 attcatattt ttgtagcata ggagacacat atccatgaag tatcaaactc aagcactggc
     3421 ttatccatat tttgtcgctg cactggtatt atttctagtg caaattctgg caggaattat
     3481 ggctggctca atttacgtat ttccaaacct tctatccgag atcacgccat ttcatatcct
     3541 gcgcatgatc cataccaatg cattattagt ttggttgttg ttgggttatt ttggtgcgag
     3601 ttatttccta ataccggaag agagtgagcg ggaaattcac agttccagat tggcctggct
     3661 acagcttggg ttttttgttt ttgctggact cgctgctgtc gctagttatt tatcaggagt
     3721 gcacgaaggc cgggaatttt tagagcaacc attgtggatc aagattctga tggttgttgc
     3781 atttctaatt tttatctata acgttaccat gacagtgctg caaggacgca agactgtggt
     3841 atcgattgtt ttgttgctag gattgtgggg agcagccatt tttttcatgt ttgcatttta
     3901 taacccggac aatctttcag tggataaact ttattggtgg tgggtagtgc atgtgtgggt
     3961 cgaaggtgtc tgggagctca tcatgtcggc tatgctggca tttctattga tcaaaatgac
     4021 gggggtcgat cgtgaaatta ttgaaaagtg gttgtatgtc atcgttggct tttctttatt
     4081 tagtggattg ctaggaactg gacatcacta ttattggatt ggtacgccgg aatactggca
     4141 ttggatcggc attgttttct ctttcctcga agtcgttcca ttttttgcca tgattgtgtg
     4201 gtctttttat ctggttagaa aaagcggccg taatcacccc aataaagctg ctttattttg
     4261 gagtttgggc tgtccgatca tggcgtttat tggtgccgga ttactgggat tgatgcatag
     4321 ttttccatcg atcaatttct atacgcacgg cacacaacta acagcagcac atggacattt
     4381 ggctttttat ggtgcctatg tgatgctgaa tttggctttc tttacctatg ccatgcctat
     4441 tctccgtaat tcgcagccgt tcaatcagaa tttgaacatc ttaagcttct ggctcatgaa
     4501 tgctgccatg attggcatta ccttcgcttt gttatttgcg ggcatcttac aaacgcattt
     4561 gcaacgtgtg atgaatctcg gctatatgca ggtgcaatcg caacttgaat tgttttattg
     4621 gttacgcttg ggttccgggg tctttttcat tgttggtgcg ctgatattca tctactcggt
     4681 gctagggccc aaccgtgaag catttgttgc cggaaaattc cagcactata acgtctgatg
     4741 gcacatgatt cggtgtttgc cgcagcaaaa ggaccagtat ctgcgcaatc tgctactgaa
     4801 attcctttct atcacgaaac aggtaatgaa tgtgctttgt ttgcagcagc ctaccgcgaa
     4861 ggattaccac tactacttaa agggcctacc ggttgtggca aaactcgttt tgttgcccac
     4921 atggcagctc gtctcggtcg accgctccac actgtttcat gtcacgatga tctaaccgca
     4981 gcagatttga ccggacggta tctgctgcaa ggtggtgaga caacatggct agatggccca
     5041 ctaactcgag ccgtgcgtga aggcggtatc tgctatctag atgagattgt cgaggcgcgt
     5101 aaggatgtta cggttgttct ccatccgctc actgatgatc gccgaatcct gcctctagag
     5161 cgtaccggag aatttttgca agcaccggat gattttatgt tggtcgtatc ttacaacccc
     5221 ggttaccaga acattttaaa atcattgaag cctagtacgc ggcaacgctt tgtcacgatc
     5281 aatttcgatt ttcctcctcc cattattgaa actaggattg ttgctaccga gagcggccta
     5341 gcagaagaac ggtgtcgtct tttagtcgat ttggcacaac gcatacgagt gctgaagaac
     5401 attgatttag aagaagttgt ttccacgcga ttgctgattt attgcgccat actgttaaaa
     5461 aatggcttcg atccctatca ggctgcgcag gccacgctga tcgaaccgct atgcgaagat
     5521 gatgatgtga aacagggatt gctggaattg gtccgcgcta catttggtta atctatgaat
     5581 tggcttaatt ttactgagtt agaggaacag gctggtcggt tttggcatcg gatcgttgga
     5641 tcagcagaaa gttacccgaa ctaccaacag gccgctatca cgctggatca ggtacgccca
     5701 gcgctgggta ttttctttcg tggactaggt ggtgcttcag gtgttgctat tcaaagtgga
     5761 tccccacggt catccggtca tcgcctaacg ttgcgccaac gactggggtt gatgcaggag
     5821 tcactgaatg atattaactg caacacccag cgtatattgt tgccagcttc actggcttat
     5881 tttccagatt cgaacctgaa tcgtttacat tatttctggc ttgcggcatt tttcgctaca
     5941 gcccgagaag ttttgtatca gttgggaagc actaaaacag ctcaacaaac tggagaagct
     6001 tgcattgatc gcctgcaaaa tgatttggct ttcctgcatc aggcctactg gatcagctct
     6061 accgtttgca ggcttaatcc tggtttgcta gttatttatc aacaactttg cagtgctgtt
     6121 cttgagcaac ggccacaccg tagacttcct aatcaggagc gagtaattga agctgtgatc
     6181 cgaaggttac tcgggcagtc agattcctct gacgaagtag atgcttgttg gctttccata
     6241 caacaaacca ctcccgattt ttcatcgtgg caagctggaa aaaactatca accttttctg
     6301 ttggtaccgc tttggggcaa catagacatc aatcaggcaa tcgatgccgc caagcttgat
     6361 ggatccaatg attccatcgg caacagcggc gaagcgtgca actcgcagca caaaaaaacg
     6421 gcacaccgga ataatcaaga tcaaagtaga cgtgacgacc ctctactttt gaatcggttc
     6481 gaaaaattat tgagttggtc ggaaatggtc aatgttaacc gacctgtcga ggatgatgat
     6541 gagcaatccg caaaagcagc tgaacaagca attgaaacta tcacattaag cccgcacgaa
     6601 gagaaagcag tcacacaact taaatttgat ctggatcttg ctccggatga catgaatcca
     6661 gaaatgctga taggtgagtt tacttatcca gagtgggatt ttaaaggcat gcaataccat
     6721 cctcggcaat gccgggtaat ttttcaacgc gcacagtcca gctgtgaaag atgggcactg
     6781 gatcaacaaa cccaaaaacg tttgcgtaag atccgtcgtc agttcgaaac gctgctaccc
     6841 aagcgagaat tacttcgcgg tcagtacaat ggcgccgatt tggacatgga cgcattagtg
     6901 cgatctcact gtgattttct ggcgactggc aattattccg aaagtattta tcgcaacact
     6961 cgcaagcagt cacgtgatct gagcgttgct atcttgatcg atgtttcctt atccacggat
     7021 agctggattg atggctaccg cattctggat ataggaaagg aagcactgat cacccttgcc
     7081 accggattga ctgcttgccg agatagcttt gcgatctaca cctttacgtc gcgtaagaaa
     7141 gattatatct gtgtcaccga agtaaaaaaa ttcgaggagt cgttcaattc acctgtgtta
     7201 caacgcatcg ctgctttgca gcccggctat tatactcgta tgggtgctgc gctgaggcat
     7261 gtgcaacagt tgctgagtca gcgccaggag cgccatcgcc tactgctaat gctgaccgat
     7321 ggtaagccaa atgatctgga ttattatgaa ggtcgctacg gtattgagga tactcggcaa
     7381 gctattttgg aggctcggcg tagtggatta tcagtttttg gaatcacgat cgacaacgaa
     7441 gcacaggatt atttcccctt tttgtttggt agggggggct acactatcat atctcgaccc
     7501 gaccggttat cacatttatt gccagccatt tatcagcagc taatcaattg atagatttca
     7561 atcagtcttg taacttttgc gattacacta catggaagtg aacaatgagt tagatgacct
     7621 tggtcaattt attgaagttt tgagagtttt gaaggaacac ggtgtcaaat gagaaataca
     7681 tacacacggt atctggttct attgaatttt acggttgctt gtttgaacgg ggacggaaat
     7741 agtctgaatt gtcataataa gtagcattgc tattattggg ggaccaatct gggtagccta
     7801 gcaggggaat cggggttaga tcagtggaag ttatagattt ggttgagagt aaatttgcca
     7861 gtttgttatc taggctcacg atctgactat aaaggtcttg ctgataaaac gacggctcaa
     7921 tgtgaaaaat cagcccttta cccgtcatac cgatataggg ggatagcgct ttttcataaa
     7981 gactatgtcc aaataggaaa aatttcatat gttggagcaa gtcgctacga ttttcccaaa
     8041 ataatttttt ccattcaaaa tttttcagca aatatgttaa atcttcatgg ctactcgtta
     8101 ctaaaatacc ggactcatcg agtagtgtaa tagcgtctct taatcttcct cgggccgatc
     8161 gatgctgttt ctcagctatt tttagcgtat ggtagtgaag tgcattgagt tgcgcttttg
     8221 attgaggaaa agtcatccaa acgagtgcat tgaaaagatc gtgccaattc tgtgctcttg
     8281 tctggagttc accgcataaa taggttcttg gctcatattg ctgttcaaaa agcgatgtcg
     8341 gtgtggtttg tgttacaaat cgcagcggat ggtgagtatt cgttcgaata tttgatttct
     8401 gaaccgatag caaatgattc aattgatcag gggttggcca ctgttctaat tgattaagta
     8461 attgaccagc ctgacgcaaa ggctcaaaca tcggggagca atcaaggaaa tgatgtttcc
     8521 attgatcgat gtgttcacgc ttcattcagg taatgaatga acctggaaag cgagtaggat
     8581 actaagcaca gtggctatag ctgcaatgac acccacagta tcgtaatgca taatggaacc
     8641 tgtcggagtg gtgctcatga tagaaccagc aaaaagggaa gcaataccgg cagatagttg
     8701 ctgaatcgaa atgttgaagc taagaaaact tccacgtagt cgaggggtaa cacttgcggt
     8761 aactaaagcc atggcgggga caaatctgcc tgaaacgagc atcatgaaaa gtgttgatac
     8821 tgctattgcc aatagaaccg aggccgtact cagatgagtg atcatcagaa ttggaaacac
     8881 tgataatgcg gctgcaatac taaatatctt atgcttgccg tgctgatcgg agagtttgcc
     8941 aaataaccgt gccgtataaa aagttgctaa tccaccgcag aaataaagat aaggaatatc
     9001 gacttcagac atgccaatat tggttatcat atacgttgta atgaaaggac tgaccgaaaa
     9061 acctgcgaac attagcatgg caatcaatgc aaaggcgtat aaatgattac ggttataaaa
     9121 aactgcgcgt aaatgttgtg caggatggcg gtgttggcga tgcttgagat gaccgtgtaa
     9181 agaaggaagc aaccatattg ccaagacaat cattaccaga ctgattaatg ttagaaataa
     9241 gaagggcgca cgccaactaa acaagttcac caataatata cccaaaggca ttccaataac
     9301 cgctacagca gagaaggctg acatgacagt tcctgtcgca tctccccgtc gatgtgcggg
     9361 ggtcacatcg gcaacgatgg taaaaatggt ggcgctaagt acgccactaa acccacctgc
     9421 gaccattcgt gctaataaca ataaataata ctcaaacgag agtgcacata gcaatgttgc
     9481 taagccaata ccaccgcaga gaaaaataag gaaacttttt cgattgtaat ggtcgataat
     9541 gaatgaagca aagaaactga acgcacctgc actaaaagcg taaattgaaa tcaataagct
     9601 aaattcttga ggtgtgagtt caaacaattt cattaattgc ggaccaagcg gcaataacat
     9661 catgaaatcc atgatatgga taaaatgaat tcctgcgagt actgcaataa tggggcgttc
     9721 ctgaatgggt gtaaaatgag gaacggtacc taattcaact aactcagagc tttcaccggt
     9781 ttgcagaatt tgttcagtat cgtgcatttt gttttaccgg tttgtcagtg ttatgtgcaa
     9841 agctggaaca aataataaga tgtattttaa gttgatcagt aataaaggaa ttttgaacat
     9901 gtgccagaag tgtatggtaa cgagcgatca ttgtaattca aattatccgg cttcaattgt
     9961 gcaagataag cacaaagatc caactaaacg tgaatttatc aaacgaacct cagctgtact
    10021 aatgtcttgg gggttgttgg caaccggatt agttaatgct tccgaatcga aaacggtgaa
    10081 tgagaaaaaa agaatcatac ctaaaccaga aaatgtatta acaccggatc aagcattaga
    10141 aagattaatg caaggcaatg aacgttatgt atccggaaat tctaaaccat tagatttcca
    10201 tgatgtaaaa acctcattgg tcaaaggcca aaatccttat gcaaccattc tggggtgctc
    10261 tgattctagg gtgagtcctg agcattgctt cgatgaagca ctgggtgatt tatttgtagc
    10321 gcgcggcgca ggtaattatc taacgaatga taatttagcg accattgaat attcggttgc
    10381 agtattaagt acaccattaa tcatggtgtt ggggcacgaa agctgcggtg ctgtgaaagc
    10441 ggccattgat gctgtagacc gtcaccgtga ttttccaggt catattcaat tgctagcaag
    10501 tgcaattgcg cccgcagtta gaacagtcag cgagatagca aaagttagcg atacgacgga
    10561 agatcgcttg attcacgtta ctaagcaaaa cgtaatcatg acggttgaac ggcttcgcga
    10621 gaaaacacct attcttgatt attaccatga tcgtaaaaaa ctgaaaattg ttggcggaat
    10681 ttatcatctc gacacaggaa gagtggaact cattgcttag cttagaattt ccttagtgat
    10741 tgattcttta ctaaacagta gttattgcta atatcagaat cgagtccagt cttcatgaac
    10801 aataacccca ttatcgatga taaagcgata agtgtattgt tcaactttgt agatccaaac
    10861 ttcccgtaca ctataacctc gtgaaccact atgataaggt agataaaggc cgcgttgcgt
    10921 tacaaatggg ctacgagcaa gtggttgacc taagttaata atcagtttat caactaaatc
    10981 gcctttagaa attaaagctc cattaggggc gcgaaatccg cataatagaa gtaccataaa
    11041 catcaataag attatccttc tcatgatagt ctccttgcaa ttggttggtt gatcactcga
    11101 actgttattc aatcgttagt aataacaata cgagatgacc cctgaatgca aactgaataa
    11161 tctggttttt ttgaagatac tttggagctg atttaggttt tctggcttcg ccagatgaca
    11221 gcaagaagac ttcctaataa tgaatgcatc aaaactgaaa ttgcgccaac tactggtgca
    11281 agcgggagca gtggaaatgc ctgtttcgag agcacaatgg ctaaaccgga attctgcata
    11341 cctacttcga tcgaaatggt gcgcatactt tgcttatcat gatttgaata tctggccaag
    11401 gtataaccca agaaaaaacc acctgcatgg agtagtgccg ttgctaatat cagttcacca
    11461 atataatgac ggattccttc tgcttgaccg gcaaaaacag ttgcgcagat tatgcacaca
    11521 ccgataacgg ccaatagtgg tgcaagaggc atgatacgtt gcacagtgct agggagccag
    11581 tgattgagtg tgatgccgag tgcgatcggt aaaataacaa cttgtagcgt ttgtttgaat
    11641 aacatccaag tatccactgg cactaaagta ccagcaaata attgagtgag taggggtgta
    11701 agtatgatag cagcgagcgt cgaacaaacg gtcatgacga ctgaaagcgc aacgtctgct
    11761 ttggcaatat atgaaaccaa atttgaagcg gtaccccctg ggcagcatcc gactagaatt
    11821 aaccctaccg caaaatgaat cggcaagttt agtaacattg cgattcccca ggcaagaaca
    11881 ggcataatgg tatattgccc caaaaaacca gctgcaacgg cttttggcat gcggccaatt
    11941 cgacgaaaat catcgatggt aagcgtcaac cccatgcaaa gcattacaaa agctaggata
    12001 agcacgacgg ttgatccttg attgaggggt gtaaaccatt cagggttgaa taatgaaaag
    12061 gtagctgata tcagcatcca caatgggaaa agcagtgtta atttttgaag cataccgatg
    12121 atgaagtgga attaaatctt gggtaaattc acaggagaga ataacagggt gtagtgttgc
    12181 ccatagcgac taataattct ttgagtatgg agtccagcag caattgccat gttttgcaag
    12241 gtagaaaaag tttcagggta gtcatgatca tgcacatggg tacaagcttc ttcaagttgt
    12301 tcgggtggaa ctttagtcca agtttcccgc atgaattgta aatagtgatt taagtaggtt
    12361 tcgcggtctt gatgttcttc acgtacgaca tcgactaaga taagccatcc ttgttttgac
    12421 aaggatcggc ccactgcatt gaaaaatcgt gttttatcct ccggcgtaag gtgatgaact
    12481 gcaaatccac taaaaatgat gtcccattga tcttgagttg attcgatcga tgttaacaaa
    12541 tcattatggg ttaatttaac gggacaagta agtggggcta gataggtttt agcctcggtc
    12601 agcgctgttt ctgaaagatc aaccccttca taatagctgg gtgggtattt cagtaaacag
    12661 ggtgccatga agcgcgcatt tccgcatcca agatccatta aacgatagcg tgtaacgtct
    12721 tgccgtaatc caagcaattt ttgtacaccc tcatagattt cttggtgaaa catatagttg
    12781 tatgcagtaa tgaggtcata caacgaccaa gattgagtaa aaatggttgt cggttggttg
    12841 gtgctcataa aagtcaattt acaataatca ggttttagtt cgcctattct ttagtgtttt
    12901 tatcctcact gtcaaaaata attaacacca acgcggtaca gttattttta ccgtattttt
    12961 gaatagggaa aacccctcta aaggagaaga aaatgaatag cgcacccatg gaagcaagca
    13021 atacagctcc tatcgaattt aaaccaatag ctaatgaggc atttgctgta atgattgtgt
    13081 cggaacaagg gttaatttac gattgtagcg agaagggggc aagtctgctt ggttggatgc
    13141 aacatgattt aattggacat tcagttgcag aagtaatacc gaaattagcc tcgattactt
    13201 tgctgaaaca gggtagagct aacgcctatt tacggttttt atcacggata ggtcatcatt
    13261 ttgagttgat tggaaaaaat gatatgcgta cgacaggaga aatttttttt agtgatgttc
    13321 agcaagaagg ggttcattat cttcttttaa cgatacagct aaattagtat atcgttgtat
    13381 gcattaaagt gtagtatttg cgacgattgt tgaaaaaaac aattctatgc cgaggctatt
    13441 ttcccctgca attctcgata acgagcacac tttctcatac tgttttaaag tgctggtatt
    13501 aggatctgct cgcacttcaa caagtaattg ataatagtct tagtcttcgc ggtatttatc
    13561 cacataaaag aactgaatat cttgcgctaa aaaaacagga tgatttcaga tgcttcctac
    13621 attctctgca ctagagttag aaaactctct ctttatcacg tataattcgt acaactatgt
    13681 attgatttag ctattaccta cttagcgtga tagccttttt tgaaagtatc ctaaaaggag
    13741 tattttgtgt tgcagcaacc tcgtgccatc cttgttttag ctgatggtac agtttttcaa
    13801 ggtatttcga tcgggataaa aggaataact gtaggggaag ttgcttttaa cactgccatg
    13861 acgggttatc aggaaatttt gaccgatccg tcttactgcc agcaaattat tacactgact
    13921 tatccccata tcgggaatac cgggtcgaat aaagaagata tcgaatcagc aggtagcgtt
    13981 aaggtttttg ctgcaggttt ggtaatccga gatttatccc ttaccgcaag taattggaga
    14041 aaaacggaat ccttaccaac ttttctggaa aaccataatg ttgtcgcaat tgcaggtatt
    14101 gatacgcgaa aactcactcg cattttaaga gagaagggcg cacaagcagg ttgtatcatg
    14161 gctggcgata tcaatattga agctgcgctg caacgtgcac aaacttttcc gggattggta
    14221 gggatggatt tagcaaaagt agtcagctgc cagcatgctt accaatggac tgaatcagac
    14281 tggcggttag gctatggtta tgcatcacaa ccgactattg attaccggat tgctgcactc
    14341 gatttcggcg tcaaacaaac catattacgt agattagcaa gtcgtaactg tagtgtaacc
    14401 gtttatccag cacaaacacc tgttgaagaa attttgctct ctaatcctga tggagttttt
    14461 ctatcgaatg gcccaggcga tccagaacct tgtgattatg cgattgatac cgtgagaaga
    14521 ttaattgata tgaaaattcc aattttcgga atttgtcttg gacatcaaat cttagcgctg
    14581 gcagcaggtg caaaaacaat gaaaatgaaa tttggtcatc atggcgcgaa tcatcctgtt
    14641 ctggatatcg atagtgggaa agttattata accagtcaga atcacggttt tgctgttgat
    14701 atgaatcaac taccaacgaa tatccgagta acccatttat cgttatttga taaaagcttg
    14761 caagggtttg agttaatcaa tagtcctgcg ttttgttttc aaggtcatcc agaagcaagc
    14821 cctgggcctc atgatgcgag ttatctattt gatcgtttta tcgatcgaat caaacaatat
    14881 aagaagcaac agagtcacgt tgcgaagtaa gacttaaact aagcctttgt tgctgcaata
    14941 taaatgccaa aacgtactga tattaaatcg attcttgtga ttggagcagg gcctatcatt
    15001 attggtcaag cttgtgagtt tgattactcg ggtgttcaag cttgtaccgc gctacgggaa
    15061 gaaggttatc gaattgtact ggtcaattca aatccagcga cgatcatgac agatccagaa
    15121 atggcggatg cgacctatat cgagcctata acctggccca tgcttgagaa aattatcgat
    15181 atcgagcgtc cagatgcact cttaccaacc atgggtggac aaacagctct aaattgtgcg
    15241 cttgatttag ttaaatacgg agtgctcgat aaatatggag ttgagctaat tggcgcatcg
    15301 cgagaagcga ttgataaagc ggaagatcgt gagaaattta aacaagcgat ggctcatatc
    15361 ggattgagct ctgctcgctc tgcgattgct cacagcatgg aagaagcact gcaagtacag
    15421 gcgatgattg gttatccagt aattattcgc ccttctttta cgctcggcgg aacgggtgga
    15481 ggaattgcgt ataatcgaca agaatttata gaaatttgtg aacgcggatt ggatgcctca
    15541 cccactcgcg aattactgat tgaagaatct gtaattggct ggaaagagtt tgaaatggag
    15601 gtaattcgtg ataagcaaga taattgcatt atcatttgct cgatcgaaaa cttagatccg
    15661 atgggtattc ataccggtga ttcgattacg gtcgcgccgg cccagacact cactgataaa
    15721 gaataccaaa tcatgcgtaa tgcctctatt gcggtattac gtgaaattgg cgttgaaacg
    15781 ggcggatcaa atgttcaatt cgcgattaac cctgctgatg gtcgtctgct agtgatcgaa
    15841 atgaatccac gtgtttcccg atcttctgcg cttgcttcta aagccactgg atttcctatt
    15901 gccaagattg ctgccaaact ggctgtaggt tatacattga atgaactcag taatgatatt
    15961 accggcggtg ttacgcctgc ttcttttgag cccacaatcg attacgtcgt tactaaaatc
    16021 cctcgatttg catttgagaa atttcctcaa gccaatgatc gactaacgac tcagatgaaa
    16081 tcagtgggtg aggttatggc gatcgggcgc acatttcaag agtcatttca gaaagctttg
    16141 cgtggacttg agattggcgt tgatggttta gatgaaaaat catccgattt agatttgata
    16201 gcggctgaat taggacatcc tggtcctgag cggatttggt atgtagccga tgcatttcga
    16261 catggcatgg cttttgatga aatttatcaa ttaacacaca ttgacccctg gtttttgcat
    16321 caaatagaaa atcttatcca gcaagagcaa tctttgactg atagtacagt ggattctatt
    16381 gatacggact tattgcgcca attaaaaagg agtggattct cagatcgtag attggccaaa
    16441 ctactcaatt caagtgaatt agcgattcgt acccgtcgcc taaatgaaaa aatatacccg
    16501 gtatataagc gtgtcgatac ctgtgctgct gaattttcca cagatacagc ctatctttat
    16561 tctacttacg aagaggaatg tgaagctaat cccagcacta gaaagaagat tatggtgttg
    16621 ggtggaggac cgaatcgcat agggcaggga atagaattcg attactgctg tgtccatgcg
    16681 tcactcgcat tacgtgaaga cggctatgaa acaatcatgg tcaactgtaa tccagaaact
    16741 gtttcaacgg attacgatat atcagatcgt ttatattttg agccgcttac actagaagat
    16801 gtcctgaata tcgttgctgt agaaaagccg aatggagtta ttgttcaata tggtgggcaa
    16861 acgcctttaa aactagcgag agatcttgag cgctttggtg taccgattat tggtacaagc
    16921 cctgatatga tcgattgtgc agaagatcgt gaacggtttc aggatttact taatcgctat
    16981 aacttgaagc agcctatgaa tcgagtagca cgcaatgttg aaatggcatt ggctgctgca
    17041 actgaaattg gctacccttt agttgtgcgt ccgagttatg tattgggagg tcgcgcaatg
    17101 gagattgtgc acgaacagaa agaccttgag cgatacatgc aagaggcggt caaagtaacg
    17161 aatgattcgc ctgttttact tgatcattat ctaaataatg caattgaggt tgatgtcgat
    17221 gcgatttttg atgggaaaac gatactcatt ggtggcatca tggaacatat tgaagaagcg
    17281 ggtgttcatt ctggtgactc agcttgttca attccaccat ttagcttatc aactgaatta
    17341 cagcaagagc ttcgtcggca gacagaagtg atggctcgtg cattgaatgt gatcggatta
    17401 atgaatgtgc aatttgcaat tcaaaacgat attgtatatg tactagaagt aaatccaaga
    17461 gcttcgcgca cggtaccctt tgtatcgaaa gctacaggcc tccaacttgc aaagatctcg
    17521 gcacgatgca tggttaagaa aagtttatat gatcaagcca tctttaaaga tattgagcct
    17581 acattcttct ccgtcaaaga agcagttttt ccattttcta aattttctgg tgtagatact
    17641 attttagggc ctgaaatgaa atctaccggc gaggtcatgg gtgttggaaa cacctttgct
    17701 gaagcgtttg taaagtcaca gttggcgaca gggattaaat taccaatctc tggtaagatt
    17761 tttattagtg tgcgcgcgag tgataagtta gaagccgtta cacttgctaa gaaatttatt
    17821 gagcttggct ttacaattta tgcaacgcaa ggaaccgctg aggtgatgat tgcgtcgggt
    17881 ttgaatgttc ataaagtaaa taaagtagcg gaagggcggc ctcatatagt cgatatgatc
    17941 aaaaatggtg aaatttgctt gattatcaat acagtagcta ataaacgaag agctattcaa
    18001 gattcttact ctattcgaca tgctgcttta ctcgcaaaaa ttacttatta caccacaatg
    18061 gccggtgcga gggctgctta tatgggtatt tcaaatatgc gtgaattgca tgcttatagt
    18121 atgcagatgt tgcatcacca aacaatacag taattcgatt tcttattacc gacaaggata
    18181 aaattcataa tgagtccaat ccctttgacc gttgcgggtg ctgaaaaatt acgtgctgaa
    18241 cttcacgaaa tgaaaacagt gcagcgtcct gcagttattg cagcgattgc tgaagctcga
    18301 tctcatggag atctttcaga aaatgctgaa tatgatgctg caaaagaacg acaaggcttt
    18361 attgaaggta gaattgctga actcgaaagt aaactcgcga atgcacaaat cattaatcca
    18421 gcattgattg atgccgatgg tgcatgtgtt tttggtgcaa cagtcgaatt aaaagatttt
    18481 gggggaaatg taaccgtaat ctatcaaatt gttggagatg atgaggcgaa tctcaaagag
    18541 aaaaaaattt ctattagctc accaattgct cgtgcgctga ttggaaaata tgctggagat
    18601 atcgttgaag tcgttgcccc tgcaggcgtt cgggaatacg aaatattgaa tattcagtat
    18661 atctaatatc ttcaagaaat cgttaataac attgataatt attttattct cttattctcc
    18721 taaataaata tacctccctg agttcaattg taagtcttga aatccttact ttttatttgc
    18781 tttaaaaaat aatcaattaa aagatgtcgc tttgatgatc ataatgattt attaataggg
    18841 tgattatcat tggcaattat ttagctcttc tatgaaatca aataaagtta atcgagcgtg
    18901 gattcatgag cacgttaatg atttttatgt taaacaagcg cagaaagcgg gttatcgatc
    18961 gcgggctgct tataaactca ttgaaattgc tgagcgcgat cagcttttta gtccgggaat
    19021 gatcgttgtt gatttggggg cagcccctgg cagttggtct caagtctgcc gtgaaagaat
    19081 cggtaaatcc ggtaagatta ttgcacttga tttattaaat atgcttccac tagatggggt
    19141 agcgtttatt caaggtgatt ttagtgaaga aaaagtttgc gagatgattg agaatcaatt
    19201 ggcaggagat ctggcagatc ttatcctttc agatatgtca cccaatctcg caggaattgg
    19261 aatgagtgat caagcacgca gtatcaatct tgctgaattg gcgttagcat ttgctaataa
    19321 taaattgaaa ccaggaggga gttttcttgt caaaattttt caaggaagtt atgttgatca
    19381 atacatccga gatatgaggt ttcattttaa taaagttatg atacgaaaac caaaagcttc
    19441 acgttccaaa agtaaggagt tgtatttgtt aggattaggt aaaattatct gttagactgc
    19501 tatttatctc acagaaataa tttaaatttg ttaataaatc gtgattactc tagcaaaaga
    19561 atactaaacg aatttattat taatacgaat aataggaata aacattctag tactttaatg
    19621 tataaattta ggatctacta tgaataattt aataaaaaat atggctattt ggctagtgat
    19681 tgctatagtt cttatgacag tattcaatca gtttactgta agacagtcgg cgcaatcacc
    19741 gatagagtac tcacaattca tcaatgaaat gaatcaaggc aggatcgcga aggtaactat
    19801 tgatgggcgc acattaaaag gtactaaatc agatgggcgt caatttataa catttgcacc
    19861 atcagatccc tggttagtca gcgatttact tagagcgggc gttatcgttg aagctaaacc
    19921 tgaagaagaa ccttcgatac tgatgagcat ttttgtatca tggtttccaa tgctgttatt
    19981 aattgctgtt tggattttct ttatgcggca aatgcaagga ggcggtcgta gtggcggtgc
    20041 tttctctttt ggtaaaagca aagcaaggat gttggataaa aatagcaatc atgtgacatt
    20101 tgctgatgtg gcaggttgcg aggaagcaaa agaggaggta gctgagttag tcgagtttct
    20161 gagagatcct accaaattcc aaaaattggg tggtcgtata cccagagggg tattgatggt
    20221 tggcaaccca ggtacaggta aaacattatt ggctagagcg attgccggag aagctaaggt
    20281 tccttttttt agtatttctg gctctgattt tgtggaaatg tttgttggtg tgggcgcatc
    20341 tcgagtaaga gatatgttcg aacaagcgaa aaaacatgcc ccatgtatta ttttcattga
    20401 tgagattgat gcggtaggtc gtcagcgtgg agcaggtctt ggtggtggta atgatgagcg
    20461 tgaacaaaca cttaatcaac tccttgttga aatggatggt tttgaaggga atatgggggt
    20521 gattgttatt gctgcaacta atcgtcctga tgtacttgat ccagcgctgt tacgtccagg
    20581 acgttttgac cgtcaagtga ctgtgccact tcccgatatc cgaggaaggg agcaaatttt
    20641 acaagttcat atgcgcaaag ttcctatttc accagatgtc catgctgatg ttttagcgcg
    20701 aggaacccct ggaatgtctg gtgcggattt ggcaaattta gttaatgaag cagcactgtt
    20761 tgctgcacgt tctaataagc gcctagttga tatggatgat tttgaacgag caaaagataa
    20821 aattctaatg ggagctgagc gacgttcagt tgttatgcca gaaaatgaaa gaagaaacac
    20881 tgcttatcat gaatcaggtc atgcagtcgt tgcttatttg ttacccaaaa ctgatccagt
    20941 tcataaagtt tcgattattc ctcgtgggag agcattaggc gttacgatgc aattgccgac
    21001 tgaggatcgt ttcagtatgg atcgcgatca aattttacaa acaatttctg taatgtttgg
    21061 tggacgcatt gcagaagaaa tatttatgaa tcagatgaca acaggtgctt caaatgattt
    21121 tgaacgtgcc acagaaacta cgcgaagaat ggttacgcaa tggggtatgt ctgatgtgtt
    21181 gggtccgatg gtttatggag aaaatgaagg tgaagtgttt ctgggtcgtt cagtaactac
    21241 acataaaaat gtcagcgaag ctacaatgca aaaggtagac gcagagattc gccgtattat
    21301 tgatgaacaa tatgcacttg cacgaaaact catcgaagag aatagagata aaattgaagc
    21361 gatgactaaa gcattgcttg agtgggaaac tattgatagc gagcagatta aagatattat
    21421 ggagaatagg ccgccacgtc cacctcaacc accacaatct aaatcatctg cagcacccaa
    21481 agaagacaat aaaaccgtat cttcaacagc tgcaaatgag ggatctgcac aagaagtata
    21541 agcttcttat cgtccaatcc tcttttaaat caaccgtaga tatactttga tatatctacg
    21601 gtttttttct gttctttttt tgtatctaag cttgcaggta tagggctatt tctagattca
    21661 ttttttcctt tgaataatca cattaatcta aaagaaaatt taaagaaaga tacgccattg
    21721 attatgggta tcataaatgt aacgcccgat tcattttccg atcagggacg atatgctgca
    21781 tcagagaaag cgattgagca tgctattaaa ttgatagcgg atggtgcaga cttgcttgat
    21841 attgggggtg aatcttctcg acctggtagc gcttgcgtca gtcttgatga ggaactcaac
    21901 cgtgttattc ctgttatcga agctttaaaa tccgcagcta ttcctatttc agttgatacg
    21961 accaaaccag aagtaatgca agctgctgtt caagcaggtg tttcaatgat taatgatatt
    22021 aacgcactta gaacggaagg tgcattaaaa attatcgctg agagtggagt tatggtatgc
    22081 ttgatgcata tgcaaggact gcctaaaacg atgcaagata atccacttta cgctgatgtg
    22141 gtttctgagg ttagggagtt tttgcagcag cgaattcaaa tcgctcaaga ttggggcatc
    22201 tcaaatgatc aactagtaat cgatcctggt tttggctttg gtaaaacttt gcaacataat
    22261 ttagaattat ttcgatcact tgatcaattt actactctta atatcccggt tatggtagga
    22321 gtatcacgta aatcgatgct aggaacaatt actggaaatg acgtcaatca ccgcacttat
    22381 gcgagtattg ctgcagccgt attagctgca gaaaagggag ctaaaatttt gcgcgtacat
    22441 gatgtcaaag caacaaaaga agcaatttta gtttttaatg cattaaatcg atgatttaaa
    22501 ataagatagt ccattaataa attactaatt tctcattcag aatatctgac taatgaagaa
    22561 aaaatatttt ggaacagatg gaatacgagg acgtgttggc gaatttccaa ttacacctga
    22621 aattattctc cacttaggtt attcagctgg taaggtattg ttagaatcgg attggcacct
    22681 aaaaaaggga gaaaagcctg ttgtattaat tggaaaagat acgcgtgtat ctggttattt
    22741 attagaatca gctttagaag ctggattttc tgcagcaggt gtggatgtac tcttatcagg
    22801 ccctttacca acaccagcga ttgcctattt aataagagca ttacggctac aagcagggat
    22861 tgtaatttcc gcttcccata atccatttga tgataatggt gtcaaatttt tctcatctga
    22921 aggtacaaaa ttaccggatg aaatggaatt aaaaattgag atgggacttg aagcaccgat
    22981 tcggacgatg ccttcagtaa agcttggtaa agtgcaacgc ttaaaagatg cgactggaag
    23041 atatattgag ttctgtaaaa gcgctttccc ttatcattta gatttgagag gattgaggat
    23101 tgtcatcgat tgtgcaaatg gagcaactta tcatatcgca gggcatgtac tccatgaact
    23161 aggagcagat gtagtgacta tctgtgcaaa ccctgacgga ttcaatataa atcaccaatg
    23221 cggcgctact cattgtgcta ctttgcaatc tgccgtcaaa gaacatgcag ccgatttggg
    23281 tatagcctta gatggagatg gcgatcgagt aatcatggtt gataatgatg gtgttatcta
    23341 tgatggtgat cttttaattt atatcattgc taagcatcgc tacagtaaag gcatgctaag
    23401 agggggagta gtaggaactt caatgactaa tctcgctata gaaaaagcga tgatgctatt
    23461 gaatattcct tttgaaagat ctaatgttgg tgatcgatat gttcttgaac tgctcaataa
    23521 aaacggatgg caactcggtg gggaaagctc cggccatatc gtttgtttag acaagcatac
    23581 caccggcgat ggtattattt ctgcactcca agttctgtac gcactccgtg attcaaaatt
    23641 gaacttaaga gaagccactg gtgatattaa actatttcca caacgcctta ttaatgttcg
    23701 ggtagcaaaa ggattgaatt atctcagcaa tccgtctatt caggccatcg ttcaacaagc
    23761 tgaaaatgat ctaaacagtg agggccgagt gttacttcgg gcatcaggaa ctgagccatt
    23821 aattcgagtc atggtagaag gcagtaatca gcagaaagtt aatagttggg ctgagaaaat
    23881 cgctaattct gtacaagaaa ttgctcaaca acaagaatca aaacaatcgt aattgatggt
    23941 cattattaaa ttgaaagcga atcaaaaatt attacatgta acaattctgt aataattatt
    24001 aattaatatt atcttttctt aaagtttaag gaaaaggtaa atggtgattc tgatcaattt
    24061 gatcaacttg ctttaatatg gagagattta ttttgaacat gctgaataaa agaagtttta
    24121 ttagcgttat tggtggggga ctattactta ttttaagtag ccagcttagc gcaagagcga
    24181 ttgtgatgat tgatgggtct agtacggtat accctattac tgaagcagtt gccgaagatt
    24241 ttcaagcaat gaaaaaaggt gaaatcagag taactgttgg aatatctggg acaggtggag
    24301 gatttaaaaa gttttgccgt gctgaaattg atattgctaa tgcgtcaaga cctattcaaa
    24361 aagcagaaat ggaaacctgc aaagcggctg gtgtcgaata tattgaatta cccgttgcgt
    24421 tcgatgcatt gacaatcgcc ataaatccta agaacacatg gagtaaaacc attacagttg
    24481 ctgagttaaa agcgatgtgg gagccttctg cgcaaggaaa aatcactaaa tggaatcaaa
    24541 ttaatccagc ttggccagat gaaaaaatta aattatatgg cgcaggcgct gattctggga
    24601 catttgatta ctttacagaa gcaatcgtag gcaaagccaa atcgagtcga ggtgatttta
    24661 ctgcttctga agatgataac gtacttgttc aaggcatcgc tagtgacaag tttagtttag
    24721 gtttttttgg atttgcttac tatattgaga atcaaaaaaa ggtaagtgct gctgctgttg
    24781 ataatggcaa agggggggta actccttcag ctgaagctgt tgagaatggc agttatcaac
    24841 cgttatctcg acctattttc atttatgtca acgcaaaagc attagataaa ccggaagtca
    24901 gagaatttat caatttctat atgaataatg cgcctgcttt ggtaaaggaa gtaaaatttt
    24961 ttccattacc agctgaggtt tatcagctta acttagatca tttggaaaaa agaaaaattg
    25021 gcaccgtatt cggtggagaa agtaaaattg atctcagaat cgaagaatta cttaaattag
    25081 aaaagcagat gtaaatttga catttctgaa cgattttgaa caataacttt ttaaattgat
    25141 tactgataag ctttaggctc tagagaattt taatcaacga aagacttaac ttgccaaatc
    25201 atcttgctcg gtgatcatat ggattttttt cctatctttc ttaatatccg agatcgaaaa
    25261 tgtttggtag ttgggggcgg agaaatagct gctagaaaag tagagctatt gcttaaggca
    25321 aatgctcttg tcacagttat ttctcccaca ttaggcgaaa cattagaagc gttttcgtct
    25381 caatctcaaa tcagttacat tcgagatatt tttcacccta aatatctcac gaattacacc
    25441 ttagtaattt ctgctactaa taatcatcaa gtaaatcagc aagtctggga agaagccatc
    25501 aaagcaaata tccccgttaa tgttgtcgat aatctgagct tgtgttcttt catcatgcca
    25561 gcaattattg atcgctctcc agtggtaatt gcaatttcca gtagtggtaa atcacctgtg
    25621 ttagttcgaa tgctgcgcac ccgtttggaa accctaatac caggtgctta cggtaagttg
    25681 gcacaatatc tgggggaatt tcgtgatttg gtaaaacaac atttcatcct tccagagcaa
    25741 cgcagatttt tctgggaaaa gcttttgcaa agtccatttg tcgaaatggt tctttcaggg
    25801 aaagaccagg ccgctcagca atacctcata aaatcaattg agaatgaccc aaaagatcta
    25861 ccgcagggtg aagtttactt agttggcgca ggccctggaa atccggattt attaacatta
    25921 agggctttgc aattaatgca gcaagcagat gtcattgttt atgatcgtct tgtatcgaaa
    25981 agtataatgg atctcactcg acgtgatgca acccgaattt atgcgggtaa agagcgtaat
    26041 aatcatacgt tatcgcaaga atcgattaat gagttattag tgcgtctcgc caaagacgga
    26101 aaacgtgtgc tacggttaaa aggcggtgat cctttcattt ttggtcgggg cggggaagaa
    26161 atagaaacct tatctgagca tcgtatcccg tttcaggtag tacctggaat tactgcagct
    26221 tcaggggtgg cctcatatgc aggtataccg ctgacccatc gtgattatgc tcaatcctgt
    26281 acttttgttg caggacatct taagcataat agcttagatc ttgattggaa tactttagct
    26341 cgacctcagc aaacgatagt ggtatatatg ggtttactgg gattgcctat cttatgtgaa
    26401 cagctcattg cccatggcct acctgctaca acgcctgcag ctatcgtgca gcaagctacg
    26461 accaataatc aacgcgttgt aatcgggtct ttagatacac ttccttccct tgcgatgaat
    26521 gcacatattg tgcctccgac acttattatt attggtgaag tcgttaagct tcatcagaag
    26581 ctaacttggt atcactatga ttattgaatc ctactaaaac tttattgagg agtattcttt
    26641 tatacaatat tcttaaattt cttacactta acattgcgca caatattaat ttgtcactaa
    26701 gtgataatat gcaactgata atttactcaa ctcataagtc cattcactca aagcttaaga
    26761 tattattcaa ataggttgta ttcacacgct gaacagtgta ccaagcgctg ttttcgtttt
    26821 attttcttca tattttaatt gtaatgacaa ttcgtgaaca ggcaattgca gggattaaat
    26881 ggacagcagg aggaaaactc gctgctcaag caattacttg gggtatcaca ctcattgtta
    26941 tgcgattgtt acatcccgag gattatggac tattagctat agcatctgtt ttcctagcat
    27001 ttatgatgat gctctctgaa gctggacttg cgccagcgct tgtgcaaaag caggaattga
    27061 gcaaaatagc attacaacag gtttttgcga tcattatcgt tatcaattta gcattattag
    27121 cattactaaa tttattggca ccaattatcg ctaatttttt caatgatgaa cgccttattt
    27181 taattctaaa cgtgatgtct ttacagtttc taactgtaat cgccataacg attccacaag
    27241 tattacttta tcgcgagctt aaatttaaat acttatcgat tattgatttc atcgctgtta
    27301 ttaccgggag tttgtttaca ctagcgttgg ctatattaga ctatggtgtt tgggcgctga
    27361 tatacggtaa tctaataact gcagtttgta aggtaattgc gttcaatata ctaacaccta
    27421 cctatttgct tccaaaattt tcgttaatcg gtatgcgcgg cttcttgtct tttggcggga
    27481 atattacact ctcgcgcttg ctctggttct tctttacgca aattgatgta attatcgttg
    27541 gtaagttact cggtaaagag ttattgggct attattcgat agcaatgcat ctggcatcat
    27601 tacccgttca gcggatttca tcaatcattg ggcaggttgc ttttccagtt tttagtaagg
    27661 tacaagatga tcagcaacag tttcgtaatt ttgttctgaa atcgatccgt atcctgtctt
    27721 taattgcttt tcccgtatta tggggaatat cgagcgtagc taatgaaatg gttttacttt
    27781 ttttaggtga aaagtggttt catgcaattc taccgttaca attactttct ttgatgatgc
    27841 catttcgtat gatagccaat ttcctaccat ctgcaactga tgcgctagga catccagaaa
    27901 tcggggtaaa gaatgttttc ttggctgcta tagtaatgcc aattgcaatt ttaattggtg
    27961 ttcaatgggg aatcgttggt gttgctatcg cttgggtcac tgtttacccc attatccttt
    28021 ttattaacta tcatcggtca ctgagcgtaa tcggattaaa gcttaaagat ctgttttatg
    28081 tgctttttcc agtcttaata tcgagtgttg ggatgtattt aatggtatgg ggagtggatt
    28141 tactcttaga gaatcaacta aataaattac ttcaacttac tattatgatc ttatctggag
    28201 gactggctta ttatggattg accataaaat ttaacaatgc tggctaccga gattttattc
    28261 aattatttaa aaagaattaa ataatttatc gtataaattt ttacactatt aatgaaacag
    28321 tgcaaacaga actcaaagta gacttttacc tttataaatt tttatgcttt aaattattgc
    28381 catggcaatt ataaataaaa aaagtggttt atttttaatt agaatacttg ttcttattat
    28441 tgtactagtt tgggtatgga tgcctgagtt tcttttgtgg catgttaact tgaaattacc
    28501 cacggctgaa atcatcaatg aatctcgatc attgccttca actagcacgc ttattgaatt
    28561 ggggcaaatg gatcttaaac ccccatcaaa cccattagat gatagtcaag taattatatt
    28621 ggctgaaaaa gctttaaacg gcattttgag tgtaccaggt ttcgatgata ttgctgcgtc
    28681 acctattttt aatgaaagtg atttaaatca aggatcagct ctgcaacagc ttatatttgc
    28741 aagccttatc acagttgatt atttgcttga agcctatcgt attacccatc aagataaatt
    28801 ttatgtcgca gcattgaaat caattcaagc cttttgtaat tttgaatcgt cacgcttcct
    28861 tgatattggt tttttatgga atgctcatgc aattgcagca agagttcctg tattgattaa
    28921 attctggtta tattatcgcg atcgcgagga ttttgaagtt attctggcgc aacagttatt
    28981 taaattaatc gttagaagtg gtttattatt ggctaaacct gagcattatt cgtggagaac
    29041 gggtcacggt at
//
LOCUS       DAOQKQ010000006        28770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_656_length_28770_cov_6.093122, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000006 DAOQKQ010000000
VERSION     DAOQKQ010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 28770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28770
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_656_length_28770_cov_6.093122"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PKZ49_00725"
     CDS             <1..371
                     /locus_tag="PKZ49_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HND35112.1"
                     /translation="GRICEGLFEFWLAPFTLFIWQKIDSFNRLITARRNPNLILLTAS
                     WFVGRPDIGFILVAGWHILSTGFLAWRLFKAWQAKHEQGTLTSWMEAIDPVLDRKQIA
                     VKVFTRVPLAEKDDQNRARA"
     gene            358..1353
                     /locus_tag="PKZ49_00730"
     CDS             358..1353
                     /locus_tag="PKZ49_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylglycerol kinase family protein"
                     /protein_id="HND35113.1"
                     /translation="MHALSSTSTSTSLRIGLIYNPLGGLARKHHHAISTILSELPEVL
                     QWEISAVDDFDSAVNALIQSKIEWLIIIGGDGTIQGVFTRIFSRLPTSQWPSISIIPG
                     GTTNMTAFDLGIHDKPNLILERLKQHLNQSTNNSVLKTINKAVMRIEQTGQPTLYGMF
                     LGLGLIARGVKFSRSRIKQIGITGNIFTTIIVLRSLIGTFLGRPHPTWAPVQIMLPHT
                     NEANRSQPYLLAMISTLDRILMGIHPYWGQEKASLHATFVAQHCTRFWLAMLKIILKQ
                     GRSLKKEHGYLSYNTESLELLLDDEYIVDGELFYASSQQGPLKISAKEMVKFLIL"
     gene            1368..2339
                     /locus_tag="PKZ49_00735"
     CDS             1368..2339
                     /locus_tag="PKZ49_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35114.1"
                     /translation="MNETSMKSPQLPPALIEEMAALSAQAVSIEFDAFVSALKQRFGD
                     SLDAILLYGSCLHTTDLAEGVADFYVLVDDYQNAYAEHYLQFFNRILPPNVFYLEVNY
                     QEKIYRSKYAVLTTSHFEKAASDWFHPYIWARFAQPSRILSARDEIIRQRINTALAQS
                     VVKFLRTTLSAQPENTLPVEAIWTNGLMLTYAAELRTEKQTRAQKLTQLSLNEYTRLT
                     TAALPALQDMMERSESQHYYHIRSTRFKNNLTKFQWWLRRWQGRILSILRLGKATLTF
                     RDCADYAAWKIERHTGVRIEVTPMLRRHPILLGYKIIWKLFRRGIVR"
     gene            2379..3035
                     /locus_tag="PKZ49_00740"
     CDS             2379..3035
                     /locus_tag="PKZ49_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2959 domain-containing protein"
                     /protein_id="HND35115.1"
                     /translation="MRQLFRWSTWFTYGLLLTLTACSTMYYSGLEKIGIPKRDVLVHR
                     VEKARDTQEETKEQFKSALEHFTAVTNFKGGDLEATYKKLNDEYESSIAKAAEVRDRI
                     SDIEDVSQALFNEWEQEIAQYTNQAMKSSSQQKLTATRSHYKQLINTMKQAETKIQPV
                     LAVFKDQVMFLKHNLNAKAIASLKGELGTLQSDVSTLISSMEKSINEANSFISKMEQK
                     "
     gene            complement(3209..3682)
                     /locus_tag="PKZ49_00745"
     CDS             complement(3209..3682)
                     /locus_tag="PKZ49_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35116.1"
                     /translation="MYTINHNKTIEIVNFLLERYCSLGYPVDIKETTKVLSLSKDDVL
                     ISGVCIDKATDGRIFPYTVMDNFLEELMCGESCCSVGRFVGNSKMTLKDIVFFWSYIH
                     QFMLSEGITRLLSTATERHARHYSNLLNWEIYSAPIYIPEYRVRARLLKYDLKQF"
     gene            complement(3876..4349)
                     /locus_tag="PKZ49_00750"
     CDS             complement(3876..4349)
                     /locus_tag="PKZ49_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HND35117.1"
                     /translation="MPIIFLLIFLGCAGLIGYAIYLQLFEDLLPCPLCVAQRIAYWVI
                     GITALLAYFNQTRSAIVKICRGLVIFFAMIGAVIAGRHAWLIRFPESFECGISPEEAF
                     LNALPLAQWWPTMFEANGDCADTSWKFLFLTIPDWSLITFMLIGITALLSGLKRK"
     gene            complement(4366..5574)
                     /locus_tag="PKZ49_00755"
     CDS             complement(4366..5574)
                     /locus_tag="PKZ49_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HND35118.1"
                     /translation="MDDLIFFATCPRGLESVLATELAQLSGTSIEVVQGGVIFRGNWQ
                     TCYRVNLESRIASRILWRIAQQNYRSEADIYRITFQLPWQTWFSPKLTIRVKVSAIKC
                     ALRSLDFVTLKIKDAVCDKFRKIKNTRPDVDTANPDIRIHGFLDQQQLTLYLDTSGEA
                     LFKRNLRKSSGNAPIRENLAAGILALTGWKPGVPLLDPMCGSGTFLMEAAQISSRIAP
                     GINRHFAFEQFTLFDSPVWKQLKEAALNQQLPCTSQLIFGSDLSGYAIADARENLISN
                     HLAEIVQLKQANILEISPPHQLGGIIVMNPPYGVRMSEQQLLADFYPKLGDRLKKKFS
                     GWCAYILTADPSLPKLIRLNPSRRTPLFNGALECRLLEYRVVEGSMRKGEPRSSDSVS
                     ANLAVISSDQ"
     gene            complement(5615..5989)
                     /locus_tag="PKZ49_00760"
     CDS             complement(5615..5989)
                     /locus_tag="PKZ49_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HND35119.1"
                     /translation="MSRLFLALGAINAFLCVLLGAIGAHILKQKFSGELFNTFQTGLQ
                     YHFYHALGLILIGVLLFHLPQSKLVKLSGWVMLTGILFFSGTLYIVSFTEMRSLGMVA
                     PIGGLSLMSAWLILAYAIWSEK"
     gene            complement(6076..7419)
                     /gene="xseA"
                     /locus_tag="PKZ49_00765"
     CDS             complement(6076..7419)
                     /gene="xseA"
                     /locus_tag="PKZ49_00765"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HND35120.1"
                     /translation="MDHLSTASLNVHTLLSVSELNSYARALLESSFPLLWIRGEVSNL
                     KQYPSGHWYFLLKDKDAQIRCVMFRQKNQWLDWSLRDGMQIEVQALVTLYEARGEFQL
                     NVEHIRQAGLGNLYEAFEKLKSKLQATGIFDAIHKKNIPEFPKRIGVITSLNTAALHD
                     ILSTLRHRMPSLPIIIYPTPVQGKDAAKSIVTMIELAGKRAECDVLLLCRGGGSIEDL
                     WAFNEEIVAYAIFHSVIPIVTGIGHETDFTIADFVADKRAPTPTGAAQIVSPDTQEIA
                     KHLNHLYHRIERAYKHEIERRMQTLDLLCHRLIDPRESIKHQVNRLQYLREKLLFCWS
                     RKIEKIDSENRHLQRRFFSSALNLEHMLNQQFERSSKLHKAINYYLERQGTKLVHYQT
                     QLTHLNPKSVLQRGYSITSNAEGKILRDSSQINIDDSIQVTLAEGWVKAKVSDAG"
     gene            7628..8035
                     /locus_tag="PKZ49_00770"
     CDS             7628..8035
                     /locus_tag="PKZ49_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrosocyanin"
                     /protein_id="HND35121.1"
                     /translation="MKLFKALCASIVLGLAVANVQAGEQNFTVVINAYDTTIPELNVE
                     GVTVKNIRAFNVLNEPETLTVHKGTSVKITVENKSPISEGFTIDGYGIQEVIKAGETK
                     TISFVADKAGAFTIWCQLHPKNIHLPGSLNVIE"
     gene            complement(8153..8731)
                     /locus_tag="PKZ49_00775"
     CDS             complement(8153..8731)
                     /locus_tag="PKZ49_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HND35122.1"
                     /translation="MSNHQSSTGKIIAPELETIGWLNSAEPITLASLRGKVVLIHAFQ
                     MLCPGCVQHGIPQTKKIFEEFDPSKVAVLGLHTVFEHHAVMGRDALEVFAYEYRLSFP
                     IGIDKPNEHHVIPHTMMTYQMQGTPTTILIDKTGHLRMHKFGHVTDFVLGLSIGTLLA
                     EVDDESELASPSQQQNQKNLQSGACGPDECAV"
     gene            complement(8917..9510)
                     /locus_tag="PKZ49_00780"
     CDS             complement(8917..9510)
                     /locus_tag="PKZ49_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HND35123.1"
                     /translation="MSQFKKTLLCSLVSVFVSGLVVGQASAGQHDGHHGHHKTYAHFN
                     KDGELLTPKNYREWIFVGSPVTPKDMNDGNPAFPEFHNVYIDPTSWAHWKQTGTFRDG
                     TIIVKEMVSVGTKESSSGNGYFQGEFLGIAATVKDSKRFANSPNNWAYFGFASYESAS
                     AAAQPDDACAACHKANAAEDNVFTQHYPVLRAGKAKK"
     gene            9656..10261
                     /locus_tag="PKZ49_00785"
     CDS             9656..10261
                     /locus_tag="PKZ49_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HND35124.1"
                     /translation="MDIITAIKSRRAIKSFDPNHILSDTEIEQLLSLAICSPTAFNIQ
                     NWRFVVIQDKALRKKIRAVSWDQAQVTDASLLIILTADLKSWEKQPERYWKNAPQSIR
                     DYIIPAIDHYYREHSQVERDEAMRSCGMAAMTLMLAAQGMGYASCPMDGFDFEAVSEL
                     IHLPSDHVIAMFVVIGKETKPAWPQGGQLPLEEIIIYNRFD"
     gene            complement(10262..10951)
                     /locus_tag="PKZ49_00790"
     CDS             complement(10262..10951)
                     /locus_tag="PKZ49_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HND35125.1"
                     /translation="MNNFSKFLIYSLTSALFLILLVSCGDDSATSKGGAGNSSMKLMD
                     EVTSITELPSVLQESLSSEEKADLLKKILPMSDSSISPDEQFQKLREDASAGNAEAQN
                     GLGTLFYTGHAISRDASGKILDFDLESAAAWFYRAAEQGHAGAQFNLGLMYAEGQGVP
                     KDSAKAVELFTKAAEQGNVDAQNNLGVMYFVGEGVSRDVNKAKQWFEKAAAQGNEEAK
                     GNLEALQNAGS"
     gene            complement(11057..11818)
                     /locus_tag="PKZ49_00795"
     CDS             complement(11057..11818)
                     /locus_tag="PKZ49_00795"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HND35126.1"
                     /translation="MISIIQLTRMFLLAMLMFFLGLSAAWASNVESDELVDWASRGNF
                     TRIKTLFSETGKQPEQETLQRAYLVAAKNGHDKIVQFFLDQGVNIEVRDEKGYTALMY
                     AAEDDREQVMRVLLDAKAEINAANEEGMTSLLLASYRDRIEILRILIGAGANINAATN
                     KGITPTMLAAQNGYQIILQDLIKAKADLNSKAENGVTALMLAAQNGHQSATRTLIAGG
                     ANINIEAENGATALLLAKKYNHKGVIDLLVQAGAR"
     gene            complement(11854..12975)
                     /locus_tag="PKZ49_00800"
     CDS             complement(11854..12975)
                     /locus_tag="PKZ49_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HND35127.1"
                     /translation="MIELAPYAISFQYSRGRLFKEDPPVGRSEFQRDRDRIIHSTAFR
                     RLEYKTQVFVNHEGDLFRTRLTHSLEVAQIGRSIARNLQLNEDLVEAIALAHDLGHTP
                     FGHAGQEALNRCMQEYGGFEHNLQSLRVVDFLEERYAAFNGLNLCFETREGILKHASL
                     KNARKLGEVGERFILKQRPSLEAQLTNLADEIAYNNHDVDDGLRSGLITLLQLQEIDI
                     FARHLHKVRKQYPDISGRRLIHETVRSMINSLAIDLTLQSKQNIAITKPASPEAVRTA
                     LPLIEFSEEISREQQVLKSFLRENLYKHFQVIRMSNKAQHTITRLFDAFSTEVRLLPN
                     EYQQKFSNYGHQAIADYIAGMTDRFAIKEYQRIFAISEN"
     gene            complement(12968..14077)
                     /gene="aroB"
                     /locus_tag="PKZ49_00805"
     CDS             complement(12968..14077)
                     /gene="aroB"
                     /locus_tag="PKZ49_00805"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HND35128.1"
                     /translation="MANIETIKVHLVSSQSDRSYPIYIGSELLEQVEYILPYLRQKRV
                     AIVTNETVAPLYLKKIKDALEHHQVSTVAIILPDGENYKNWETLNLIFDALLKQNCER
                     KTTLIALGGGVIGDLTGFAAATYLRGIPFVQVPTTLLAQVDSSVGGKTGINHPLGKNM
                     IGSFYQPLLVLADSSTLFTLPDREFRSGLAEVVKYGLIRDAEFFNWLEVNMSKLVARD
                     LAVLNTAICRSCEIKAEIVAADEHESGVRSLLNLGHTFGHAIENAMGYGQWLHGEAVA
                     AGTIVAAELSKRMGLINETEFTRIRGLFELANLPISLPAISPSHYLDLMKLDKKVEGG
                     KMRFILLNQIGQAAIFDNIRESLLIETMLACGAND"
     gene            complement(14113..14724)
                     /locus_tag="PKZ49_00810"
     CDS             complement(14113..14724)
                     /locus_tag="PKZ49_00810"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HND35129.1"
                     /translation="MHFSKALNRRNIETQSIKRNIFLIGMMGAGKTTIGKSLANYLDK
                     DFIDSDHEIQLRTGVKIPLIFEIEGEAGFRKRETEVLQDLVKLENIILATGGGVILSE
                     ENRRLLKQNGIVIYLCASVDELLRRTRTDKNRPLLQTEDQHARLTELLAHRDVLYRNT
                     ADIIIDSGKQGVRSLVYKLIRKLRTYDVKQQYAVPLEHANERT"
     gene            15103..17214
                     /gene="ovoA"
                     /locus_tag="PKZ49_00815"
     CDS             15103..17214
                     /gene="ovoA"
                     /locus_tag="PKZ49_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-histidylcysteine sulfoxide synthase"
                     /protein_id="HND35130.1"
                     /translation="MNTTLFQRTPILDGGDPEAKREEIRRYFHTTLDRYELLFETLVS
                     DEAYYSKPITLRHPLIFYLGHTATFFTNKLILARAITHRINPRLESMFAVGVDEMSWD
                     DLDTTHYDWPMVDEVRHYRSVMRETVDRLITELPITLPIGWDSPWWTIIMGIEHERIH
                     LETSSVLIRQHALKFVKPHPHWQPCKKTGIAPENQLVTVPAGLVQIGKTIADATYGWD
                     NEYGSHHAEIPSFQASRYLVSNKEFLSFVEANGYDTDQYWEPEGLGWLRHTRAQHPTF
                     WVKQDSHWYLRLMTEVTPMPWDWPVEVNYHEAKAFCNWKTAHDKLPVRLPTEDEWYRL
                     YDSSNLSDVPATQLASGNIHLDYYASSCPVNEFKHGDFFDIVGNVWQWTETPTYPFTG
                     FEVHPHYDDFTTPTFDDRHNLIKGGSWISCGNESLKSSRYAFRRHFFQHAGFRYVVTE
                     TPALMQGSHYETDKLMSEYAEFHYGDSYFNVPNFPATLASIAITAMGNRPAHKALDLG
                     CASGRSTFELARHFEYVTGVDFSARFINQGVQLIQQGLLRYTLTDEGDLVFYKERSLA
                     ALGLESVKSKVEFYQGDACNLKPILTGYDLILAVNLIDRLYDPAKFLVNVHERINLGG
                     LLLIASPYTWLEEHTKRESWIGGFKRDGESFTTLDGLKSILGNHFKLIQGPQEVPFVI
                     RETRRKFQHTLSEVTIWEKIS"
     gene            17211..18371
                     /locus_tag="PKZ49_00820"
     CDS             17211..18371
                     /locus_tag="PKZ49_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HND35131.1"
                     /translation="MSVNFDEIIQRAGTNCTKFDKRLAVFGDSHVTPLWVADMDFAAP
                     HEVTAALISRAKHPIYGYTFAPDSLYEALISWVKQRYHWSIKQEWIVLCPGVVPSLTA
                     AILALSEVNESTIILPPVYFPFFSAVTKSNRQLTLCPLSYHNQRYEIDFDRLAALEDN
                     SRILLFCSPHNPVGRVWPQSDLEQLIKIAQKKNWIIISDEIHADLTYPEYQHTPLARL
                     AEEFENIITALAPSKTFNIPGLNLSVLIVPNAKFRQAILNFFDQLHINPANPFSITAF
                     ETAYQKGANWLDELISYLSCTRDQVADYLTKYLPAIQLVKPEGTYLLWLDCRSMRMTD
                     SELKRFFIHQAGVGLSPGILFGDEGSGFMRMNIGTPRSNVLAALEKIKKAYV"
     gene            complement(18388..18783)
                     /locus_tag="PKZ49_00825"
     CDS             complement(18388..18783)
                     /locus_tag="PKZ49_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35132.1"
                     /translation="MDTTNRVFISFALKDTELRDKLIEQLKKTHPALNVVFMPEKKSW
                     ESAWKAECQGQVDGCDGAIGIITKNTSRADGQLWELRCALDSQMKLLLVGDDEICEMS
                     AKKLPELIRDEDILPWDSSSISSFLNRLK"
     gene            complement(18888..20273)
                     /gene="nhaC"
                     /locus_tag="PKZ49_00830"
     CDS             complement(18888..20273)
                     /gene="nhaC"
                     /locus_tag="PKZ49_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HND35133.1"
                     /translation="MTKSLREPSLLQALICIVGIFLTIALGLLLVGISIHILVFLCII
                     WVSIHAAYLNYSYTDIQRMMSDSIHHALPAIYIFILMGMVIASFMYSGTIASLIYYGL
                     SLLTPSIFLPVGLILCCLMSIVTGTSWGTVGTIGVVLIGIADAIHIPIFLAAGMIVSG
                     ATFGDKLSPLSDTTNLAAMTAGVGLYQHIYAMVYTTLPSFILALGLFSVIGLQFAESA
                     IPIEQISEIQIALASLFHLDLWITLLPLICMLVLSLLRFSPAICMTCSIVIAVIISIL
                     YQDKLIVEVLNALWLNTPANTGIENIDALLGRGGIYNMAWTLILSIMALALGGVLYQA
                     KFLTVLINSFINKIRRFSTLVATTITAGFVSNLGMGEAYISIILNCQLFKALYTKLDL
                     HHAILSRSVEEGTTVTAPLIPWTTAGAFYAATLSVAVLDYAPYAFFNLLNPIVSVLMA
                     IFGIGLLRGQR"
     gene            complement(20341..22131)
                     /locus_tag="PKZ49_00835"
     CDS             complement(20341..22131)
                     /locus_tag="PKZ49_00835"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HND35134.1"
                     /translation="MTSELIDPIIVGLTILGLFFAFIKEWVKPDIAVVVAVSVLMILG
                     LLDSKQVLSVFSNSAPITVATLFIMSAALEKTGCVAKLSGWIASIAGENEKKLLAMIM
                     LTALAVSPFINNTPVVMVLMPAVIAVAVKIQSSPSRFLIPLSYATILGGMMTLIGTST
                     NILVDGVARDMGLAPFGMFEITAPAIVMGLIGYLFIYLLAPRLLPIRETLAQQFMGAS
                     DRAFMTELFIPENSRLAGKTLVEAKLNQVGVTILKIFRADEAITSPSDNFRLEVGDRL
                     VVHSKSSAMMDLHSSDWIGLQNNASNHDLETLRRKDAVIIEAIVGSSSRYVQRPIRDL
                     DLLARYGIHLIAIHRKDASIGDIVDDFQLQFGDVLLVEGTPSQIKRFCENGDLFALTE
                     GKVTSTRQHKAPIALATMGGVILLAAFKILPIESLAIIGAAIVLVTGCIRSDEAYKAI
                     EWPLIVMIFGMLAISIAMRDSGLADMAARSLLSIGQGLDPWWMLLAVIFLTSVLTEIL
                     SNNAVAVLMTPIVIGMADHMGVDARPFVVGVMFAASMSFATPIGYQTNTLVYSAGNYK
                     FSDFARLGIPLNILLGAGAALLIPLFWPFH"
     gene            22532..22912
                     /locus_tag="PKZ49_00840"
     CDS             22532..22912
                     /locus_tag="PKZ49_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HND35135.1"
                     /translation="MTKKIAHIDDDPDIRDTVKRVLEMHGYQVDSYHTSSDFISSLDN
                     SEKVPDLAILDVMVESMDAGLTTYIQLHERFPKMHAIFMTSLGDTILPYFENKSQEWV
                     CILEKPVQPTILLTIIQDRLGKAS"
     gene            22947..24380
                     /locus_tag="PKZ49_00845"
     CDS             22947..24380
                     /locus_tag="PKZ49_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HND35136.1"
                     /translation="MTTANFSWNRPTQELDSTLEKKITYQKLSDGILISNIRLFIIFR
                     WAIVLGLILFQIAAIVASDTLTQLGIKDQEDWPLAVTVILTIANIAYAYALDYCQPSK
                     YNTPSINIWVQIIIDLLCLSVVVHFVGSTETPAPFFYILHIALACIFFSTLESLFVTA
                     IVSAMYSILLVIESGMFFQVPQSMLIDAHYSGEKLHKGHILVWMAALNTLFLTVWYVV
                     SRLAIILKQHEQHLREAYEQIHQAQIAKDQYAMLMTHQLKAPLDAIRSKINLINGGYC
                     GPVPTEIADVLDRIDKRGSSMAKLILDVLRLERLKTAGRNLEAIEYISISSIIDKCVE
                     KLKPVADARQVELILSIEDFRAHGIADQIEILIENIASNAISYSHVGKSVEISSIVNK
                     NQNTAVVSITDHGIGIEEKDLPNIFNEYFYSPRAVLHNQSSSGIGLSLVKIAAANNNL
                     QLKVTSEPEVGTTFFITFTDIQQIDIH"
     gene            24589..24879
                     /gene="flgM"
                     /locus_tag="PKZ49_00850"
     CDS             24589..24879
                     /gene="flgM"
                     /locus_tag="PKZ49_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HND35137.1"
                     /translation="MKINKVNQLSTNNTVNKGNQAEKINSNKSEELEDPQSNVHLSST
                     LSSLGDTTAANISKIAEIKQAMAEGRFKVNPEVVADRLLETVKELIQSNKKA"
     gene            24879..25319
                     /gene="flgN"
                     /locus_tag="PKZ49_00855"
     CDS             24879..25319
                     /gene="flgN"
                     /locus_tag="PKZ49_00855"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HND35138.1"
                     /translation="MHLSAEDKRKLLDILMAERETVLAIIDKLKKEQESLVNGKVDDI
                     FKYAAEKDFQLYHLNNLYKQQCELLQSSDSQYIILDKSNSYNSNFNQLWTDILNLISS
                     AKQLNNTNETIVSMNLQYSQNLSFALHGNYQNHALYNARGMKTQ"
     gene            complement(25402..25899)
                     /locus_tag="PKZ49_00860"
     CDS             complement(25402..25899)
                     /locus_tag="PKZ49_00860"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HND35139.1"
                     /translation="MIHDKKVNVLFVCMGNICRSPTADAVFKQYIQTAGLANHVYVDS
                     AGTHAYHTDEPPDKRAQNAARKRGYSMHELRARLVGSQDFIKFHYILAMDKNNLEILK
                     ERCPPTHHHKLDLLMKYSTQWNTLQEIDDPYYGGSYGFERVLDLVEESSQGLLTHLLN
                     HDLKN"
     gene            complement(26132..27571)
                     /gene="gatB"
                     /locus_tag="PKZ49_00865"
     CDS             complement(26132..27571)
                     /gene="gatB"
                     /locus_tag="PKZ49_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HND35140.1"
                     /translation="MQWETVIGLEIHAQLSTKTKIFSGASTVYGAKPNTQACGIDIAL
                     PGVLPVLNVGAVERAIKFGLAVGATINSPSIFARKNYFYPDLPKGYQISQYEIPIVQG
                     GSVTIQIGDIVKVVRLTRAHLEEDAGKSLHEDFHGMSGIDLNRAGTPLLEIVSEPDMR
                     SSAEAVAYAKALHTLVQWTGICDGNMQEGSFRCDANVSVRPFGTDKFGTRCEIKNLNS
                     FRFLEKAIEYEVRRQIEILEDGGRIIQETRLYDADEDETRTMRSKEDAHDYRYFPDPD
                     LLPLVISPELIAQFKQQLPELPEAKRNRYQNDYGLSDYDAAVLTTSKAISDYFETMVQ
                     SLPDQPKLCANWIMGELIGKLNKENTEILNCSIKPEQLTKLLQRIIDGTISGKAAKTV
                     FESMWQREHAGDVDTIIAATGLIQVSDQGEIEKLIDTVLEANPQQVNDYQNGKEKAFN
                     SLVGQVMKASKGKANPMQVNTMLRDKLKR"
     gene            complement(27612..>28770)
                     /gene="gatA"
                     /locus_tag="PKZ49_00870"
     CDS             complement(27612..>28770)
                     /gene="gatA"
                     /locus_tag="PKZ49_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041352745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HND35141.1"
                     /translation="PYDATVIERFNQVGAVNIGKTNMDEFAMGSSNETSFFGPVKNPW
                     DIHAVPGGSSGGSACAVAAGMAPAATGTDTGGSIRQPAALCGISGIKPTYGLVSRFGM
                     IAFASSLDQGGPMARSAEDLAMMLNVMVGFDERDSTSLQREVEDYCRDLERPLSGLRI
                     GLPKEYFEQGLSDEVAHAVKQAIDEYQKLGANIIEVSLPNAKLSIPVYYVIAPAEASS
                     NLSRFDGVRYGYRAEQYINLDDMYKKTRAQGFGQEVKRRILIGTYVLSHGYYDAYYIK
                     AQKLRRLIANDFTDVFKQCDVLMGPTTPTVAFDLGEKCDDPIQMYLSDIYTSSVNLAG
                     LPGMSIPIGFGKKSRPIGLQIIGNYFKEAQMLNVAHQYQKVTDWHLQTPIK"
BASE COUNT         8999 a         6126 c         5162 g         8483 t
ORIGIN      
        1 gtgggcgtat ttgcgaagga ttatttgaat tctggctagc cccttttaca ttattcattt
       61 ggcagaaaat cgactctttt aaccgcctta tcacagcaag acgtaatcct aatctaattt
      121 tattaacggc tagctggttt gttggacgtc ctgatatcgg ttttatactg gtcgcgggct
      181 ggcatatact atcgactgga tttctcgcat ggcggttatt caaggcatgg caagcaaaac
      241 atgaacaagg cacactcacc tcatggatgg aggcgatcga tccggtactc gatcgcaaac
      301 aaatcgcagt caaagttttt acacgagtac ccttagcaga gaaagacgat cagaatcgtg
      361 cacgcgctta gctcaacctc tacatcaaca tcactgcgaa ttggtctcat ttataaccca
      421 ttaggtggtt tagcgcggaa acaccaccat gcgatatcga ctatcctgag cgaattacct
      481 gaggttttac agtgggaaat atcagcggtt gacgatttcg atagcgcagt gaatgcgctc
      541 attcaatcta aaatagagtg gctcatcatt attggcggtg atggaacaat tcaaggtgtt
      601 ttcacgcgta ttttttcaag acttccaacg agtcaatggc cttccatctc gattatcccg
      661 gggggaacaa ccaacatgac agcctttgat ctgggaattc atgataaacc gaacctaatc
      721 ctggagcgtc tcaaacagca tttaaatcaa tccacaaata attcggtact caaaacaatc
      781 aataaagcag tgatgcgtat cgaacaaaca ggccaaccaa ctctatatgg catgttcctc
      841 ggattagggc tgattgcaag aggtgtgaaa ttttcgcgca gccgcatcaa gcaaattggc
      901 atcaccggta atatttttac aactatcatc gttcttcgct ctttgattgg cacttttctg
      961 gggcgccctc acccaacttg ggcaccggta cagatcatgt taccacatac aaatgaagct
     1021 aaccggtcac aaccctactt actagccatg atcagtacat tagatcgcat cttgatgggg
     1081 atacacccct attgggggca agaaaaagca tccctacatg cgacatttgt agcgcaacac
     1141 tgtactcgat tttggctcgc tatgttaaaa atcatcctaa aacaaggtcg atcccttaaa
     1201 aaagagcacg gctatctaag ctacaatacc gaatcactcg aattactact tgacgatgaa
     1261 tacattgtgg atggtgaact tttttacgca tctagccagc aagggccgct caaaatttca
     1321 gccaaagaaa tggttaaatt tctcatactc tgaggttaaa atttgtgatg aatgaaacta
     1381 gcatgaagtc tccgcaatta ccaccggcac tgattgaaga aatggctgca ctcagcgccc
     1441 aagctgtctc cattgaattt gatgctttcg tgtcagcact caaacaacgc ttcggggatt
     1501 cattggatgc cattctatta tatggttctt gcctgcatac aacggattta gctgagggtg
     1561 tcgctgattt ttatgtactc gttgatgatt atcagaatgc ttatgcagaa cactatctcc
     1621 agttctttaa tcgcatctta cccccgaatg tattctatct tgaagtcaat taccaagaaa
     1681 aaatttaccg ttctaaatat gctgtgctca caaccagcca ttttgaaaaa gcagctagcg
     1741 attggtttca tccttacatt tgggcacgat ttgcccaacc ctcacgtatt ctctctgctc
     1801 gagatgaaat cattcgccaa agaatcaata cagctttagc acagtcagtt gttaaattcc
     1861 tgagaacaac gctatccgca caaccagaaa atactttacc agtggaagca atttggacca
     1921 atggcttaat gctaacgtat gcagccgagt tgcgtactga aaagcaaaca cgggctcaaa
     1981 aattgactca actcagttta aatgaatata cccgcctaac caccgctgca cttcccgctt
     2041 tgcaagatat gatggaaaga tctgaatctc agcattacta tcatatccgc tcaacgcgat
     2101 tcaaaaataa cctgacaaaa tttcagtggt ggttgagacg atggcaaggg cgaattcttt
     2161 ctattttacg tctaggtaaa gccacactga cgtttcgcga ttgtgcagat tatgcggcat
     2221 ggaaaataga acgtcatacc ggtgttcgga ttgaagtgac cccgatgctg agacgccatc
     2281 ctatcctctt aggttataaa atcatttgga aactttttcg tagaggtatt gttcgttaac
     2341 aattacatta taaataatct tatctttggg agaattttat gcgtcagtta tttcgttggt
     2401 caacatggtt tacatatggg ttgttattaa ccctaactgc ttgttcaact atgtactaca
     2461 gtggacttga aaaaattggt attcccaagc gcgacgttct tgttcatcgc gtcgaaaaag
     2521 cacgtgatac ccaagaagaa accaaagaac aattcaaatc agctttagaa catttcaccg
     2581 cagtgaccaa ctttaaaggt ggagatctcg aagcaactta taagaaactt aatgatgaat
     2641 atgaatcgag tattgcaaaa gcagcagaag ttcgtgatcg aattagcgac attgaagatg
     2701 tttcgcaagc gttattcaat gaatgggagc aagaaatcgc acagtatacg aatcaagcta
     2761 tgaaaagtag tagtcagcaa aagctaacag caactcgttc acattataaa caattgatta
     2821 ataccatgaa gcaagcggaa acaaaaatac aacccgttct cgctgtattc aaagatcaag
     2881 tcatgtttct taagcataac ttgaacgcaa aagcgattgc ttctcttaaa ggtgaattag
     2941 gaacgctaca atctgacgtc agtactttga tttcatcgat ggaaaaatcc attaacgagg
     3001 ctaattcatt tatcagcaag atggagcaaa agtgatcgga gtttaaaaaa atttcactat
     3061 aactgaagag gcaggccatt gaacaatccg gcttgcttca gtatcaccca attacaattt
     3121 tttcgttact tggttagaag aaaggtaatt taatgggccc atcagattca gtgggtctac
     3181 cactaactga atctaacgaa tccatcaatt agaattgttt taaatcatat ttcagaagac
     3241 gagctctaac tctgtactca ggaatataga tcggtgctga gtagatttcc caattgagca
     3301 agttagaata atgcctagca tgcctctcgg tagctgtact tagcaagcgt gtaatacctt
     3361 cagataacat aaattgatgg atataggacc agaaaaaaac aatatctttc aatgtcattt
     3421 tactatttcc aacaaatcgt ccaaccgaac aacaagattc tccacacatc aactcttcta
     3481 agaaattatc catcactgta taggggaata tcctgccatc agttgctttg tctatgcaaa
     3541 cacctgaaat aagcacatca tctttactaa gacttagaac tttggtcgtt tctttaatat
     3601 caaccggata acctaacgag caataacgtt ctaataaaaa attaacaatc tctattgttt
     3661 tattatgatt gattgtgtac atattggccc caaaaggagc tcataaagca tttactgcta
     3721 ataccattcc tattccgaaa tttaattttc aggatataga aataaaacga aacaaactta
     3781 aagattaaaa actaaaataa ttcattgaaa attcagcgca aagcaatggt gacataagtt
     3841 ttatttatct tcaacccacc ccaatagaac aaacactatt tacgtttcaa ccctgaaagc
     3901 aaagcagtaa taccgatgag cataaatgtg atgagtgacc aatcaggaat cgtaagaaat
     3961 aaaaatttcc aactggtatc tgcacaatcg ccattcgctt caaacattgt gggccaccac
     4021 tgcgcaagcg gtaatgcatt caaaaaagct tcttcaggac taataccaca ttcgaatgac
     4081 tcaggaaaac gaatcaacca tgcgtgtcga ccagcaatca ctgcacctat cattgcaaaa
     4141 aaaataacca aaccgcggca aatctttaca atagctgatc ttgtttggtt aaaataagcc
     4201 agtaatgctg ttattcctat tacccaataa gcaatgcgtt gtgctacaca caatgggcac
     4261 ggcagtaagt cttcaaataa ttgcagatat attgcataac caatcaatcc tgcacagccc
     4321 agaaaaatca ataaaaaaat aataggcata tctaactaat gagaattatt gatcagagga
     4381 aatcacagct aaatttgctg atacagaatc cgaagagcgg ggctctcctt tacgcatact
     4441 accttctacc actcggtatt cgagtaagcg acattctaat gcaccattaa atagaggagt
     4501 gcgacgcgaa ggattcaacc gaattaattt aggtaatgat ggatctgctg tcaaaatata
     4561 agcgcaccaa ccgctaaatt tctttttaag ccgatcccct aatttaggat aaaaatcggc
     4621 aagtaactgc tgctcgctca tgcgcacacc ataaggaggg ttcatgacaa taatgcctcc
     4681 aagctgatgg ggtgggctga tctcaagtat attggcttgc ttaagttgaa cgatttcagc
     4741 caagtgatta gaaatcaaat tttcacgtgc atcagcaatc gcatatccgg ataaatcact
     4801 gccaaaaata agctgagatg tacagggtag ctgctgattc aaggcagctt ctttcaattg
     4861 cttccatact ggagaatcaa aaagcgtaaa ttgttcaaaa gcaaaatggc ggttaatgcc
     4921 tggcgcaata cgactactga tttgcgctgc ttccataaga aaagtaccac taccgcacat
     4981 aggatccaat aagggaacac ctggtttcca gcccgttaaa gcgagaatac ctgcagccag
     5041 attttctcta atgggtgcat tcccagaact cttccttaga ttgcgtttaa ataatgcttc
     5101 gccggaagta tccaaataca atgtcaattg ctgctgatca agaaagccat gaattcggat
     5161 gtcaggattc gctgtgtcca cgtctggccg ggtatttttt attttcctaa atttgtcaca
     5221 aactgcatct tttattttga gtgtaacaaa atcaagactg cgcaatgcgc atttaatggc
     5281 gcttactttt acacgaatcg ttaatttagg agaaaaccat gtctgccaag gtagttgaaa
     5341 cgtaatacga taaatatccg cttcactacg ataattttgt tgtgctatcc gccagagtat
     5401 acgactagca atacggcttt ctagattcac gcgatagcaa gtttgccaat taccgcgaaa
     5461 aataacaccg ccttgtacga cttcgatcga tgtaccactt aactgcgcta attcggttgc
     5521 aagcaccgac tcgagaccgc gcggacatgt ggcaaaaaaa attaaatcat ccatcctaca
     5581 atcaaaaatt tacattgcta acggtgtatt cacttcattt ctcagaccaa atggcatagg
     5641 caagaattaa ccaggcgctc attaacgata aaccgccaat gggggcaacc atccctagtg
     5701 accgcatttc ggtaaagctg acaatgtaaa gtgtgccaga gaaaaacaat atcccggtca
     5761 gcatcaccca acctgacagt ttcactaact tcgattgagg taagtgaaat aacaagacac
     5821 caatcaaaat caaacccaat gcatgataaa aatgatattg caagccagtc tggaacgtat
     5881 tgaatagttc accagaaaac ttctgcttaa ggatgtgagc gccaatcgca cccagtagta
     5941 cacaaaggaa agcattgatc gctcccagag caaggaataa acgtgacatc ttgatcctaa
     6001 taatttctaa aataaacgat cgattataaa gctcgtcagt ctgcactacc ccaactgaaa
     6061 agtccattga ttctattacc cggcatcact caccttagcc tttacccaac cctcagctaa
     6121 agtaacttgg attgaatcat cgatattgat ctgactacta tcccgtaaaa ttttaccttc
     6181 agcattagaa gtgatgctgt agccccgttg taatacagat ttaggattaa gatgtgtgag
     6241 ctgggtttga tagtgaacaa gcttcgtccc ttgacgttct agataataat ttattgcttt
     6301 atgcaatttt gaagatcttt caaattgctg attaagcata tgctcaaggt taagtgcact
     6361 gctgaagaat cttcgttgca aatgtctatt ttcagaatct attttttcaa tttttctgct
     6421 ccaacagaat aacagtttct cacgtaaata ctgtaagcga ttaacttgat gtttgatcga
     6481 ctcccgagga tcaattaagc gatggcataa caaatccagt gtttgcatac gacgttctat
     6541 ctcatgctta taggcacgtt ctattcggtg atacaaatga tttaaatgct tcgctatctc
     6601 ttgagtatca ggagaaacaa tctgtgctgc cccagttggg gtaggcgcac gtttgtctgc
     6661 gacaaaatca gcaatggtaa aatcagtttc atgtccaata cccgtcacaa tcggaattac
     6721 gctatgaaaa atagcatagg caacaatttc ttcattaaag gcccaaagat cttcgattga
     6781 gccgccaccc cgacaaagta atagtacatc gcattctgcc cgttttcctg ctaactcaat
     6841 catagtcacg attgatttcg ctgcgtcctt tccttgtact ggcgttgggt aaataatgat
     6901 cggtaaagaa ggcatgcgat gacgtaatgt cgaaagaatg tcatgcaatg cggcagtatt
     6961 tagcgatgta ataacgccaa tccgcttagg aaactccgga atattttttt tatgaatggc
     7021 atcaaagatg cctgtagctt gtaattttga ttttaatttt tcgaaagctt catataaatt
     7081 ccccaaacca gcttgtcgga tatgttcaac attaagctga aattcgcctc ttgcttcata
     7141 caaggttaca agtgcctgaa cttcaatttg cataccatct cgaagtgacc aatctagcca
     7201 ttgattcttt tgtcgaaaca ttacacaacg aatctgggca tctttatctt ttagtaaaaa
     7261 ataccaatgg cccgatggat attgttttaa atttgaaact tcaccccgaa tccatagcaa
     7321 aggaaatgat gattccagca gcgctctcgc atagctgttt aactcagaaa cactcaataa
     7381 ggtatgaaca ttgagtgacg ccgtcgatag atgatccata tgacaaactt attggtttaa
     7441 gcttaggaaa attatagaga aatataacga tgaataaaat cttgcgctat aattctcagc
     7501 ctaatattta actgcgatta attgctattc tcatttatct tatgttagat attagatacg
     7561 cttcactgag aagcgtaagc aacaaactta aactaactat ttataaaaaa tattctatgg
     7621 agggtatatg aaactattca aagcgttatg tgcaagtatc gtattgggat tagccgttgc
     7681 aaatgtacaa gctggagagc aaaacttcac ggttgttatt aatgcctatg atacgacgat
     7741 tccagaactc aatgttgaag gcgtaaccgt taaaaatatc cgtgccttca atgtgctcaa
     7801 tgaacctgaa acattaactg tgcacaaagg cacatctgtc aaaattaccg tagaaaacaa
     7861 atctccgatt agtgaaggtt tcactatcga cggttatgga attcaggaag tcattaaagc
     7921 gggcgaaact aaaacaattt cttttgtagc tgataaagca ggtgctttca caatttggtg
     7981 ccaattacat cctaaaaata tccacttacc tggttctttg aatgtcattg aataattgaa
     8041 aaacactaag gaataaagtt gtagcgatac aatcaaaaca ccttactttt tcggatatta
     8101 aataataaaa gccagataat ttctggcttt tatttttcat cgattatcaa tatcaaactg
     8161 cgcattcatc tggaccacaa gcacctgatt gtaaattttt ttgattttgt tgctgactcg
     8221 gagacgccaa ctcactctca tcatcaactt cagctaataa agtaccaatc gaaagaccta
     8281 gtacaaaatc cgtaacatgg ccaaatttat gcatgcgaag gtgacccgtt ttatcaatta
     8341 gaattgttgt tggagttccc tgcatttggt atgtcatcat ggtatgagga ataacatggt
     8401 gttcattcgg tttatcgatt cctattggaa aacttaaacg gtattcataa gcaaatactt
     8461 ctaatgcatc acgtcccatt acggcatgat gctcaaacac ggtatgcaac ccaagcacag
     8521 caaccttact aggatcaaac tcctcaaaaa ttttcttggt ttgaggaata ccatgttgaa
     8581 cacaacccgg gcaaagcatt tggaaagcat gtattaacac cacctttcct ctcagtgaag
     8641 cgagtgttat gggttcggca gaatttaacc aaccgattgt ttccaactca ggcgctataa
     8701 tttttccagt ggatgattga tggttactca ttttgtaata aactcttgat caaaagcaca
     8761 gctattcatt tttttacttc aaaaaactaa agggcaactt ctttttatta atcactaaaa
     8821 aagaatgtgc cctctagcaa tacttggcat taacatgaga tctttatgag tagactttcg
     8881 ccactctgaa tcccatgagc atggtcaata attaaatcac ttcttagctt tgcctgcacg
     8941 caatacagga tagtgctgcg taaatacatt atcctcagct gcattggctt tatggcatgc
     9001 agcacatgca tcatcgggtt gagctgctgc acttgcgctc tcatagctcg caaaaccaaa
     9061 ataagcccaa ttatttgggc tatttgcaaa gcgtttggaa tcttttaccg ttgcagcaat
     9121 gcctaagaat tcaccttgga aataaccatt tccactagaa gattctttgg taccgacact
     9181 caccatttct ttaacgataa tcgtgccatc tcggaaagtt ccggtttgtt tccagtgtgc
     9241 ccaacttgtt ggatcaatat agacattatg gaactcagga aatgcagggt tgccatcatt
     9301 catatctttg ggtgttacag gagaaccaac aaaaatccat tcccgataat ttttaggcgt
     9361 taataattct ccatctttat taaaatgagc ataagtctta tgatgaccat gatgcccatc
     9421 atgctggccc gcagaagctt gaccaactac caaccctgaa acaaagactg aaacgaggct
     9481 acataataac gtttttttaa actgtgacat aattaatcct taaaatgtaa gtaatgaatt
     9541 cacagaaaac agacaaacga taggactgct ctcctggcct tatggtagtc agggtagcga
     9601 aactcgtcaa ggtaagattt attttaataa ttcttctatg attggatagt tataaatgga
     9661 cataatcacg gcaataaaat ctcgcagagc aatcaaatca tttgatccca atcacattct
     9721 atctgatacc gagattgagc aacttctctc attagcaatc tgctcaccaa cagcctttaa
     9781 catccaaaac tggcgtttcg ttgtaatcca agataaagcg ctaagaaaaa agatacgtgc
     9841 tgtttcatgg gatcaagccc aagttactga tgcttcttta ttaatcattc ttacagcaga
     9901 cctgaaatct tgggaaaaac aacctgaacg ttactggaag aatgcacctc aatccatcag
     9961 agattatatt atcccggcga ttgatcacta ttaccgagag cattcccaag ttgaacgtga
    10021 tgaagccatg cgctcttgtg gaatggcagc aatgacctta atgcttgcag ctcaagggat
    10081 gggatacgct tcttgtccta tggatgggtt tgattttgaa gcagtaagtg aattaattca
    10141 tctacccagt gaccatgtca tcgcaatgtt tgtagtaatt ggtaaagaaa caaagcctgc
    10201 atggccacag ggtggtcagt tacccttaga agaaatcatt atctataacc gatttgattg
    10261 attaactacc tgcattttgt aacgcttcga gatttccttt ggcttcttca ttcccttgag
    10321 cagctgcctt ctcaaaccat tgcttcgcct tattgacatc tctcgaaaca ccttcgccga
    10381 caaaatacat aacacccaag ttattttgag catcgacatt tccttgctca gcagctttag
    10441 taaataattc tactgctttg gctgagtctt ttgggacacc ctgaccttct gcatacatga
    10501 gcccaaggtt aaattgcgct cctgcatgtc cttgttctgc cgctcgataa aaccatgcag
    10561 cagctgattc taaatcaaaa tcaagaattt ttccggatgc atcccttgaa atcgcatgcc
    10621 ctgtgtaaaa tagtgtgccc agaccatttt gtgcttctgc attacctgcg cttgcatctt
    10681 cacgtaattt ttggaattgt tcatccgggg atattgagct atcactcatg ggcagaattt
    10741 ttttgagcag atctgctttc tcctcagatg acaagctttc ttgaagtaca gatggtaatt
    10801 cagtgatact ggttacttca tccatcaatt tcattgaaga attaccagca ccacctttac
    10861 tcgttgccga atcatcacca caactaacta ataaaattaa aaataaagca ctcgttaagc
    10921 tataaattag aaatttagaa aaattgttca caatcaacct atacctattc gttctacagt
    10981 taaaataagc tcagattcta ttacagagat ataaatccta caaattacta ctcgttcaat
    11041 ctcactctgt aatgaactag cgagcgcccg cttgcacaag taaatcaatc acacccttat
    11101 gattatattt cttcgctagc aataatgctg ttgcgccatt ttctgcctct atattaatat
    11161 tggctccccc tgcaattaag gttcttgtgg ccgattgatg accattttga gctgctagca
    11221 tcaaggctgt aacaccattt tcggctttgc tattaagatc agccttcgct ttaattaaat
    11281 cttggagaat gatttgataa ccattctgag ctgccaacat ggtgggtgtt attcctttat
    11341 ttgttgctgc attaatattt gccccggcac cgattaaaat tcgtagaatt tctattcgat
    11401 ctctatacga tgcgagtagc aaagatgtca taccctcttc attggctgca tttatttctg
    11461 cttttgcatc taatagcacc cgcattactt gctctcgatc atcttctgca gcatacatta
    11521 atgcagtata gcccttctca tccctgacct cgatattgac accctggtct aaaaaaaact
    11581 gaacgatttt atcgtgccca ttttttgcag caaccagata ggcgcgttgc agggtttctt
    11641 gttcaggttg ttttccagtt tctgaaaata acgttttgat acgggtaaag ttaccacgtg
    11701 atgcccaatc cactaactca tcgctttcta cattagatgc ccatgcagca ctcaatccta
    11761 aaaaaaacat tagcattgct aataaaaaca tccgggttag ctgaataatg ctgatcattg
    11821 gttttctcct tactgattct ctttttatta atcttaattt tcactaatcg caaaaattcg
    11881 ttggtattcc ttaatagcaa aacgatcggt cataccggca atataatcag caatggcttg
    11941 atgaccatag ttagaaaatt tttgctgata ttcgttaggt agcagtcgaa cctccgtact
    12001 aaaagcatca aaaagtcgag tgatcgtatg ctgtgcttta ttgctcatcc gtatcacttg
    12061 aaaatgctta taaagatttt ctcttaagaa agatttcaat acttgttgct cccggcttat
    12121 ttcttcacta aattcaatca atggtaacgc agtccgaacc gcttcagggc ttgctggttt
    12181 agttattgca atgttttgtt tgctttgcaa cgttaaatca atcgctagac tattgatcat
    12241 actacgcacc gtttcatgaa ttaacctcct acctgatata tctggatatt gctttcgaac
    12301 tttatgcaaa tgccgagcaa agatatcaat ttcctgcaat tgcagtaacg taattaaacc
    12361 cgatcgcaaa ccatcatcga catcgtgatt gttatacgca atttcatccg ccaaatttgt
    12421 aagttgcgcc tcaagcgaag gcctttgttt tagtatgaaa cgttctccaa cctcaccgag
    12481 cttacgtgca ttcttgagag atgcgtgctt taaaattcct tctcgagtct caaaacagag
    12541 attcaatcca ttaaaagcag cgtagcgctc ctctaaaaaa tctacgactc taagcgattg
    12601 gagattatgc tcgaatccgc catattcttg catgcagcga ttaagtgctt cctgccctgc
    12661 atgaccaaat ggggtatgcc ctaaatcatg ggctaatgca atcgcttcaa ctaagtcttc
    12721 atttaactgt aaattgcgtg caatggagcg cccaatttga gcaacttcta agctgtgagt
    12781 aagtctcgtt cgaaataaat caccttcatg attgacaaac acttgtgtct tatattccaa
    12841 ccgacgaaat gcagtggaat gaataattcg atcacgatca cgctgaaact cactacgccc
    12901 tactggtgga tcttctttga ataaacgtcc tcgcgaatat tgaaacgata tggcgtaagg
    12961 ggctaactca atcatttgcc ccacaagcta acatcgtctc gatcaaaagg gattctcgaa
    13021 tattatcaaa gatcgccgct tgaccaattt gatttaacaa aatgaaacgc atttttccac
    13081 cttctacttt tttgtctaat ttcatcaaat caaggtaatg acttggagaa atcgcaggca
    13141 aagaaatagg caaatttgcc aattcaaaca aacctcgaat ccgtgtgaat tcggtttcgt
    13201 tgatcaatcc cattcgttta gataactcag cggctacgat cgttccagcc gcaacggctt
    13261 ctccatgcaa ccattgtcca taacccattg cattctcaat ggcatggcca aaggtatgtc
    13321 ctaaatttaa taaagaacga acaccacttt catgctcatc tgctgccaca atctcagctt
    13381 taatttcaca gctgcgacat attgctgtat ttagcacggc taaatcccgt gcaactagct
    13441 tgctcatatt tacttcaagc caattaaaaa attcagcatc gcgaatcaat ccatatttaa
    13501 ctacttcagc aagacccgat cggaattctc gatctggtaa ggtaaaaagt gttgaactat
    13561 ccgctaacac caataaaggt tgataaaacg aaccaatcat atttttaccc aagggatgat
    13621 tgattcctgt tttacctcct acggatgaat ctacctgtgc taataaggtc gttggaactt
    13681 gtacaaaagg tattccacgt aaataggtag cagcagcaaa accggttaga tcgccgatca
    13741 cgccgccacc caaagcgatc aaagtcgttt tacgttcaca attttgtttc agcaatgcat
    13801 caaaaattaa attaagtgtt tcccagtttt tgtaattttc tccatcgggt aaaatgattg
    13861 ctaccgtgct aacttgatga tgttccaacg catcctttat ttttttcaaa taaagaggtg
    13921 cgacggtttc attagttaca attgctacac gtttctgcct caggtaaggc aaaatatact
    13981 caacttgctc taataactca cttccaatat agatcggata agaccgatcc gattgagatg
    14041 atacgagatg aaccttgata gtttcaatat ttgccatggg aatttcgtac taagtattac
    14101 cgttgcctgc tattacgttc tttcgtttgc gtgctccaaa ggcacagcat actgttgttt
    14161 cacatcatac gttcgcagct ttcgaataag tttataaaca agcgatctta cgccttgttt
    14221 tccactatca atgatgatat ccgctgtatt tcggtagagc acatcacgat gcgctagcag
    14281 ctcggtcaat cgcgcatgct gatcttcagt ttgcaataaa ggtctattct tatcggtacg
    14341 tgttcttcgc aataattcat cgacggatgc acataaataa atcacaattc cattttgttt
    14401 taataacctg cgattttctt cgctcagaat tactcccccg cccgtcgcta aaataatatt
    14461 ctcgagctta acaagatcct ggagtacttc tgtttcacgt ttccggaaac cagcttcccc
    14521 ttcaatttcg aaaattaatg ggatctttac cccagttctt agctgaattt catgatctga
    14581 atcgataaaa tctttatcca gatagtttgc aagtgattta ccaatcgtag tttttccagc
    14641 tcccatcatt ccaatcaaaa aaatatttct tttaatcgat tgtgtttcaa tatttcttct
    14701 attgagcgct ttggaaaaat gcatttaaat cggcagataa tcaaaaagat tggaaaagaa
    14761 tgataacttc atgaatgaaa aagactttaa aacttctttt aaactaagaa aaaatgaatt
    14821 gggtaaataa cagtgataag aaatgaaaga tgaaaaataa cttaagtaaa tctaggatta
    14881 accccactga ttataagtat actatattat taattatgga tttagctgaa tcagatctcg
    14941 cgcccgctta aaaatcatat agaaaatcaa ataccccctt tcttagtcaa gtatccgttc
    15001 taaaaatccc tactgagtat tccgccaagt taattctctg caaaatttct tagaaggtat
    15061 aatagcaaat taagaaaagc gaaagttaag ttgattattt ctatgaatac cacccttttt
    15121 cagagaacgc ctattctgga tggcggcgac cccgaagcga aacgcgaaga gatacgtcgt
    15181 tactttcata cgactctcga tcgctatgag ttactttttg aaactttagt aagcgatgaa
    15241 gcctattata gtaagcctat tacattgcgc catccgctta tattttattt agggcatacg
    15301 gctacttttt tcacgaataa gttaattcta gcgcgagcta ttacacatcg aattaacccc
    15361 aggctagagt ctatgtttgc cgtgggtgtc gatgaaatga gctgggatga tttagatacg
    15421 acccattacg attggccaat ggtcgatgaa gttcgtcact accgcagcgt aatgcgagaa
    15481 accgttgatc gccttataac agagctaccg ataacattac ccattgggtg ggatagcccc
    15541 tggtggacca tcataatggg tatcgaacac gagcgcatcc accttgaaac atcttcggta
    15601 ctgatccgtc agcatgcttt aaaatttgtt aaacctcacc cgcactggca accctgcaag
    15661 aaaacaggaa ttgctccaga aaatcaattg gtaaccgtac cggctggatt ggtacaaatt
    15721 ggcaaaacaa tcgctgatgc aacctatggt tgggataatg agtatggttc acatcacgcc
    15781 gaaattccct ctttccaggc cagtcgttat ttggtgagta ataaagaatt tttgagtttc
    15841 gttgaagcca acggttacga tacagatcag tattgggagc cagaaggtct tggctggctt
    15901 cgacatacgc gagcacaaca tccaactttc tgggtaaaac aagatagcca ttggtatctc
    15961 cgtcttatga cggaagttac cccaatgcca tgggattggc cagtcgaagt gaattatcat
    16021 gaagctaaag cattttgtaa ttggaaaaca gcgcacgata aacttccggt ccgcttacct
    16081 actgaagatg aatggtatcg gctatacgat agctcgaact tgtcagatgt acctgctact
    16141 caattagcgt caggtaatat tcatctcgat tattatgctt caagttgccc ggtcaacgaa
    16201 ttcaagcatg gcgatttctt cgatatcgtt ggcaacgttt ggcaatggac ggaaaccccc
    16261 acctatccat tcacaggatt tgaggttcat cctcattatg atgattttac gacacctacg
    16321 tttgatgatc ggcacaatct tatcaaaggc ggctcatgga tttcatgtgg taacgaatcg
    16381 ttaaaatcat cgcgttatgc gttccgtcgt catttttttc agcatgccgg atttcgttat
    16441 gttgtcactg aaacaccagc gctaatgcaa ggctctcact atgaaacgga taagttgatg
    16501 tcagagtatg ctgaatttca ttacggagat agttatttta atgtacccaa tttcccagct
    16561 acattagcca gcatagcgat tactgcgatg ggaaatcgac cggctcacaa agcactcgat
    16621 ttaggttgtg cttcaggacg ttcaacattt gagctggcac ggcatttcga atatgtaaca
    16681 ggggttgatt tttcagcccg gtttatcaat caaggggtgc agttaattca acaaggtcta
    16741 ttacgctata cattgactga tgaaggagat cttgtttttt acaaagaaag atcactcgct
    16801 gctttagggc ttgaaagcgt aaagagcaaa gttgaatttt atcagggaga cgcttgcaat
    16861 ctcaaaccca tactgacagg atatgacttg attcttgctg tcaatttaat tgatcgactt
    16921 tatgatcccg caaaattcct tgtcaatgtt catgagcgca tcaatttagg agggttattg
    16981 ctgattgcct ctccttatac ttggttagaa gaacatacca aacgagaatc ctggattggt
    17041 ggctttaagc gcgatggaga aagttttacg acactcgatg gattgaaatc cattcttgga
    17101 aaccatttta aactgattca agggccacaa gaagttccgt tcgtgattcg cgagacaaga
    17161 cgaaaatttc aacatacatt atctgaagtt actatttggg aaaaaatttc ttgagtgtta
    17221 atttcgatga aattatccaa agggcaggaa caaactgtac caaatttgat aagcgcttag
    17281 ccgtatttgg agatagtcac gttactcctt tgtgggtagc tgatatggat tttgctgcac
    17341 ctcacgaagt gactgcggcg cttatcagca gagccaaaca ccccatctat ggttatacgt
    17401 ttgcaccaga tagtttatac gaggctttaa tcagctgggt aaaacaacgt tatcattggt
    17461 caattaagca agaatggatc gtcctctgcc ctggagtagt tccatcgctt actgctgcga
    17521 tcttggcact ttcagaagtg aacgagtcaa ctatcattct cccaccggtt tatttcccat
    17581 ttttttcagc agttaccaaa tcaaaccgac aactaaccct ctgccctctg agctatcaca
    17641 atcagcgcta tgagattgac tttgatcggc tagcagcatt agaagataac tcacggatat
    17701 tgctattctg ctctccccac aatcctgtag gtcgagtatg gcctcaatct gatttagaac
    17761 aattaattaa aattgctcag aaaaaaaact ggatcatcat ctctgacgaa atccatgctg
    17821 atctcaccta tccagaatat cagcatactc ctctcgcacg cctggcagaa gaattcgaaa
    17881 atatcattac cgccctagcg ccaagcaaaa cttttaatat tccagggctt aacctttctg
    17941 tattgatcgt ccccaatgca aaatttcgtc aggcaattct caattttttt gatcaattac
    18001 atatcaatcc ggccaatccg tttagtatca ccgcatttga aactgcttat caaaaagggg
    18061 ctaattggtt agatgaactg attagttact tgagttgcac gcgcgatcaa gtggctgatt
    18121 atctaaccaa atacttacca gcgattcaac tggttaaacc agaaggaact tatctgttat
    18181 ggcttgattg tcgctcgatg agaatgacag attctgaact caaacgcttt tttattcatc
    18241 aggctggtgt tggtcttagt ccaggtattt tatttggcga tgaagggagc ggttttatgc
    18301 gtatgaacat tggcacaccg cgatctaatg ttctggcggc gcttgagaaa attaaaaaag
    18361 cctatgtcta actaccgctc tctcgtttta ttttagtcga tttaagaatg atgaaatact
    18421 agaactgtcc caaggtaaaa tatcctcatc ccttatgagt tctggtagtt tcttagctga
    18481 catttcacaa atctcatcat caccaacgag taacaatttc atttgtgaat ccagcgcaca
    18541 acggagctcc cataattgcc catcagcacg gcttgtattc ttggtaataa ttccaattgc
    18601 accgtcacag ccatccactt gcccttgaca ttctgccttc catgcacttt cccacgattt
    18661 cttttcaggc ataaaaacaa cattcaacgc tgggtgtgtt ttcttcaatt gctcaatcag
    18721 tttatctctg agttctgtat ctttaagtgc aaagctaata aaaacgcgat ttgtcgtatc
    18781 cataggtttc ctctttgact tattattgtt caggtgtatt gctcaacaat acctctcatt
    18841 gctctctttg gtagctaaaa tcaatatagc atccaggtta ttaagcgtca acgctgtccc
    18901 ctgagtaatc ctattccaaa aattgccatc aagacagaaa cgatagggtt aagtaaattg
    18961 aaaaatgcat aaggagcata atccagtact gcaacactaa gtgtcgcagc gtagaaagca
    19021 cctgcggttg tccatggaat taaaggtgct gtcacggtag taccctcttc aacagaacgc
    19081 gagagaatgg catgatgtaa atctaatttc gtgtagagcg ctttaaataa ttggcaattt
    19141 agaataatcg aaatataagc ctcacccatc cctaaattac taacaaaacc agctgtgatt
    19201 gtcgtagcaa ctaaggtact aaaccgccta atcttgttaa taaagctatt gattaatacc
    19261 gttaaaaatt tcgcttggta aagaacacca cctaacgcca gtgccatgat cgataatatc
    19321 aatgtccagg ccatattata aataccacct cggcctaata aggcgtctat gttttctatt
    19381 cccgtattag caggcgtatt tagccaaaga gcattgagta cttcaacgat tagtttatcc
    19441 tgatacagaa tcgaaatgat aactgcgatc acaatactgc aagtcataca aattgcaggt
    19501 gaaaagcgaa gaagactgag aacgagcatg caaatgagtg gtaatagggt gatccataaa
    19561 tcaaggtgaa ataaacttgc taaagcgatt tgaatttctg aaatttgttc gataggtatc
    19621 gcagattcag caaattgtaa tccgattaca ctgaataaac cgagcgctaa gataaagcta
    19681 ggtaacgttg tatacaccat cgcataaata tgctgataaa gcccgacccc tgctgtcatc
    19741 gcagcaagat ttgtcgtatc agataacggt gataacttat caccaaatgt agcccctgat
    19801 acgatcattc cagcagcaag aaaaatcggt atatggatcg catcagcaat tcctatcaaa
    19861 acaacaccaa tcgtcccaac agttccccat gatgttcctg ttacaattga cattaaacag
    19921 catagtatca aacctacggg cagaaaaatt gagggggtca ataacgatag gccatagtaa
    19981 ataagactcg caatagtacc gctgtacata aagctagcaa tcaccattcc cattaaaata
    20041 aaaatataaa tagcgggaag tgcatgatga atactatcac tcatcatacg ttggatatcg
    20101 gtatagctgt aattgagata ggcagcgtga atacttaccc agattatgca cagaaaaaca
    20161 agaatatgaa ttgaaatccc caccaaaagt aaccccaacg caatggtgag aaaaattcca
    20221 acaatgcaaa ttaaagcttg taacaaagaa ggttcacgaa gtgatttagt caaagtcgtt
    20281 tacgatttag atccatcaat ccgcaatatc atgcagaacg tttagaagcg ggatagacgg
    20341 ctaatgaaag ggccagaaaa gtggtatcag caatgcggct ccagcaccga gcaaaatgtt
    20401 gagcggaatt cctaggcgtg caaaatcact aaacttatag ttccctgcac tataaaccaa
    20461 tgtatttgtt tgataaccaa tgggggttgc aaagctcata cttgcagcaa acattacacc
    20521 gacgacaaag ggtcttgcat caacccccat gtgatctgcc attccgataa cgattggtgt
    20581 catcaaaaca gcaacggcat tgttgctaag gatttccgta agaaccgaag tgagaaagat
    20641 aacagcgagc aacatccacc aaggatcaag tccttgacct atactaagca agctgcgcgc
    20701 agccatatcg gccaatccag aatcgcgcat tgcaatacta attgcgagca taccaaatat
    20761 cattacgatg agtggccact caattgcttt ataagcttca tcagaacgga tacaacccgt
    20821 gactagaaca attgcagcac cgataatcgc taaactttca attggtaata ttttgaatgc
    20881 agcgagtaaa atcacgcccc ccattgtcgc taaggcaatt ggtgctttat gttgacgtgt
    20941 actggttact ttcccttccg ttaatgcgaa taaatcacca ttttcacaaa aacgcttaat
    21001 ttgtgatggt gttccttcaa ccaataaaac atcaccaaat tgtaattgaa aatcatcgac
    21061 aatatcacca atgctcgcgt ctttacgatg aatggcaatc aaatggattc catagcgtgc
    21121 aagcaaatcc aagtcccgaa ttggcctttg tacataccgt gaactagaac caactattgc
    21181 ttcgataatg acagcatctt tacgtctcag tgtttcaaga tcatgattac tagcattatt
    21241 ttgtaaccca atccaatccg aagaatgcaa atccatcatg gcactacttt tgctatgcac
    21301 aactaatcga tctcccactt cgagccggaa attatcagaa ggagaagtaa ttgcctcatc
    21361 cgctctaaaa atcttaagaa tcgttacccc tacttggttt aacttagctt ccaccaatgt
    21421 tttaccagct aaccgtgaat tttctggaat aaatagttca gtcataaaag cacgatcact
    21481 tgcacccatg aattgttgtg cgagtgtttc tcgtattggt agtaaccttg gcgcaagtaa
    21541 atagataaaa agataaccaa tcaaccccat tacaatcgca ggcgcagtga tttcaaacat
    21601 accgaaaggc gctagaccca tatcacgcgc aacgccatcc actagaatat tggttgatgt
    21661 accgatcaag gtcatcattc cacccaatat tgttgcgtaa gataaaggga ttaagaatcg
    21721 agaaggtgat gattgaattt taaccgctac cgcaatgact gcgggcatca aaaccatgac
    21781 aaccggggta ttattaatga atggtgatac agctagcgct gtcagcatga tcattgctaa
    21841 taattttttt tcattttctc ctgcaatact agcgatccac ccagataact tagcgacaca
    21901 acctgttttc tcgagcgcag cactcataat aaacaacgtc gcgactgtaa tgggagcgct
    21961 gttactaaaa acacttaaca cttgcttgct atccaacagc cccaaaatca tcaaaactga
    22021 aacggcaaca acaacggcaa tgtcaggttt aacccattct ttgataaacg caaaaaacaa
    22081 acctaatatc gtcaagccaa caataattgg atcaataagc tctgaagtca aaaatcaccc
    22141 catttttctt tgcagaaaat tacaaaaatg catatataaa gttacaaaaa cttaactatc
    22201 actgaattat agtgatcaaa atatcacgct aacactggat tgtaatttgc gtcagctaag
    22261 attactagcg gattttcatg aaatcacttg ctcttaggaa aaagcagggt atagtatttt
    22321 gaagcatcga ttccaatagg aatgagttac ctagctatta ttcaatagat attgtgatgt
    22381 tcaatttcag tgaaaccata gatcgagctg ccctttggtt acacattaat tattaggctg
    22441 aaggtccatg ggcaatttca ctatttaatt tattgaaata acaatcaaaa cctattaata
    22501 cttctatcag aatttaataa aggaatatta tatgacaaag aaaattgccc atattgacga
    22561 tgatcccgat attcgggata cggtaaagcg tgttctagaa atgcatggct atcaagtaga
    22621 tagctatcat acatccagtg actttattag ctcacttgac aattctgaga aagttcccga
    22681 tttggctata ctcgatgtaa tggtcgaatc aatggatgcc ggtttaacaa cttatatcca
    22741 attacatgag cgctttccta aaatgcacgc gatttttatg acctctctag gggatacgat
    22801 tctgccttat tttgaaaata aatcacaaga atgggtatgt attcttgaga aaccggtgca
    22861 gccaacgata ttacttacca tcattcaaga taggcttggt aaagctagct agttacaaca
    22921 catttaccta atcggactca ttaattatga caaccgctaa tttttcttgg aatcgaccca
    22981 ctcaagaatt agattctaca cttgagaaaa aaataaccta tcaaaaatta agtgatggaa
    23041 tcctaattag caatatccga ttatttatta tttttagatg ggctattgtt cttgggttaa
    23101 ttttattcca aattgctgcc atagttgctt ccgacacgct tacacaatta ggtataaaag
    23161 atcaggaaga ttggccactg gctgtaacag tcattttgac aattgcaaac atcgcctacg
    23221 catatgcgct tgattactgc caaccctcaa aatataacac acccagtata aatatctggg
    23281 tacagataat tattgactta ctctgcttat ccgttgttgt ccattttgtt ggtagtactg
    23341 aaacgcctgc tccgttcttt tacattctcc acattgcttt agcttgtata tttttctcaa
    23401 cgttagaaag cttatttgtg acagcgattg tttctgcaat gtattcgatt ttattagtca
    23461 tagaaagtgg aatgtttttc caagtacctc aatctatgct aattgatgcc cactattcag
    23521 gagaaaaact acataaaggc catatcctag tttggatggc agcacttaat acacttttcc
    23581 tgacagtttg gtatgttgtt tcacgtttag ccatcattct aaagcagcat gagcagcatc
    23641 tgcgtgaagc ctatgaacaa attcatcaag cacaaattgc taaagatcaa tacgccatgt
    23701 taatgactca ccaactaaaa gcaccattag atgcgattcg tagcaaaatc aatttaatca
    23761 atgggggtta ttgtggtcca gtaccaacag aaattgcaga tgtattagat cgtattgata
    23821 aacgtggctc gagcatggcc aaacttattt tagatgtgct tcgtttagaa aggttgaaaa
    23881 ctgcgggaag aaaccttgaa gcaatcgagt acataagtat ttcatcaatt attgataaat
    23941 gtgtggaaaa acttaaacca gttgcagatg caagacaagt agagttgata ttatcaatag
    24001 aggatttccg cgctcatgga attgcagatc aaatcgaaat tttaattgaa aatattgctt
    24061 caaacgcaat ttcctattct catgtaggaa aatctgtaga aattagttca atcgttaata
    24121 aaaaccaaaa tacagcagtc gtatctataa ctgatcatgg aatagggatt gaagaaaaag
    24181 atttacctaa tatttttaat gaatattttt attccccaag agcagtactc cataatcaat
    24241 catcaagtgg catcggttta tcgcttgtaa agattgcagc tgctaataat aatctacaac
    24301 tcaaggttac cagcgaacca gaagtaggta ctactttttt tataaccttc actgacatac
    24361 agcaaattga tattcactga ttcaagtgca aatgatcaat taattcagaa atatgctatc
    24421 ttttaccttc ttttcttaat ttctcatgta tgacaaagaa tttaatatgc aattagacca
    24481 cacagtaaat gttcgttatc gaatttatta atcaactaat agaaagattc aagattatta
    24541 cattaatgac gtaagattgt tcaaattcta gcattcgagg ggattatctt gaaaataaat
    24601 aaagtaaatc agttgtctac caataatacc gtaaacaaag gaaatcaggc cgagaagatc
    24661 aattcaaaca aatctgaaga actggaggat ccgcaatcca atgttcattt aagctccact
    24721 ctttcaagct tgggtgatac tacggcggcg aatatcagca aaattgctga aattaaacaa
    24781 gcaatggcag aaggacgttt taaagttaac cccgaggtag ttgctgatcg tctactagaa
    24841 acagtaaaag agcttattca aagcaataaa aaagcataat gcatttatct gctgaagata
    24901 agagaaagtt attagatatc ttaatggcag agcgtgagac tgttcttgca attattgata
    24961 aactaaaaaa agagcaagaa tccctagtta atgggaaagt tgacgatata tttaaatatg
    25021 ccgctgaaaa agattttcaa ctctatcatt taaataatct ctataaacag caatgtgagc
    25081 ttcttcaatc ttctgattct caatatatca tactggataa aagtaatagt tataattcta
    25141 acttcaatca gctatggaca gatatcctta acctaatttc atctgcaaaa caactcaata
    25201 atacaaatga aacaattgtt tctatgaatc tacagtattc acaaaatctt tcttttgccc
    25261 ttcatggaaa ctaccaaaat catgcgttat ataacgctag aggtatgaaa acacagtagg
    25321 tagcttaata acttaacaag tttaaaatca attcattcgt cactaaacaa tcaagtaaaa
    25381 taatatttac tatctatatg attaattctt gagatcatga tttagtaaat gggttaataa
    25441 accctgactc gattcctcca ctaaatcaag aactcgctca aagccataac ttcctccata
    25501 atatggatca tcaatttcct gaagagtatt ccattgagta ctatatttca ttaataaatc
    25561 gagcttatgg tgatgcgttg gagggcagcg ttctttaagg atttcaagat tatttttatc
    25621 cattgccaga atatagtgaa acttaataaa atcttgactt ccaactaacc gtgctcttaa
    25681 ttcgtgcatg ctatagcccc tttttcttgc tgcattttga gcccttttat ctggaggttc
    25741 atcagtatga taagcatgtg ttcctgcgga atccacatac acatgatttg caagaccagc
    25801 cgtctgaatg tattgtttga atacagcatc tgctgttggt gaccgacaga tattccccat
    25861 gcagacaaaa agaacattga cttttttatc gtgaatcatt tagttaacac ttaaactatt
    25921 cttattatta gattagaaat cacttattaa tatgagcgaa tttattaatt tatcccagtt
    25981 aatattctag aaaaaattaa gctaactttc atcaattatt cgcaaataag atctaattcg
    26041 ttgtgtattc tcgctaaaat caccatcctg caaaagagcc taaatcgtat ttactccgct
    26101 taggaatcgt taacttacat ttccaattaa actatctttt taatttgtct ctcagcatgg
    26161 tattaacttg cataggattt gctttaccct ttgaagcttt cataacctgc cctaccaatg
    26221 aattgaaagc tttttcttta ccattctgat aatcgttaac ttgctgtggg ttagcttcta
    26281 aaacagtatc aattagtttc tctatctctc cttgatctga aacctgtatc aaacccgtag
    26341 cagcaattat tgtatccaca tctccagcat gttctctttg ccacatgctt tcaaaaacgg
    26401 ttttagctgc tttgcccgaa atagttccat caataattct ctgaagcaat ttagtcaatt
    26461 gctctggctt aatagaacaa tttaatatct cggtattttc cttgttaagc ttgccaatta
    26521 gttcccccat gatccaattg gcacaaagct taggttgatc cggtaaagat tggaccatag
    26581 tttcaaaata atctgagatt gctttacttg tcgttaatac cgctgcatcg taatctgaaa
    26641 gtccataatc attctgatat cgatttcgct tagcttcagg taattctggg agttgttgtt
    26701 taaattgtgc tatcaattcg ggtgatatca ctaacggtaa caaatcagga tctgggaaat
    26761 agcgataatc atgtgcatct tctttactgc gcatagttcg agtttcatct tcatcggcat
    26821 cataaagtcg cgtttcttgt attatcctac caccatcttc taggatctca atttgccttc
    26881 ttacttcata ttcaatcgct ttctcaagaa agcgaaagga atttaagttt ttaatttcac
    26941 aacgtgtccc aaatttatct gtaccaaagg gtctaaccga cacattagca tcacatcgaa
    27001 atgaaccttc ttgcatattt ccatcgcaaa tccctgtcca ctgaacaagt gtatgtaatg
    27061 ctttagcata ggctactgct tcagcactac tacgcatatc aggttctgaa acaatctcaa
    27121 gcagaggggt acccgctcga tttaaatcaa ttccgctcat tccatgaaaa tcctcgtgga
    27181 gtgattttcc agcatcctct tctaggtgag cacgagtcag tcgtacaact tttactatgt
    27241 ccccaatttg aatggttacg ctgccccctt gtacaatcgg tatttcatat tggctaatct
    27301 gatagccttt cggtaggtcc ggatagaaat aattttttct tgcaaaaatc gaaggtgaat
    27361 taattgttgc tcctactgct agcccaaact taatcgcacg ttccaccgct ccgacattaa
    27421 gaactggcaa aacccctggt aacgcgatat caatcccaca tgcttgggta ttgggtttcg
    27481 ccccataaac agttgaggca ccagaaaaaa tcttagtctt tgtggaaagt tgtgcgtgta
    27541 tttcaagacc gataactgtt tcccattgca tgaacaaata gcctttctaa caaatagtcg
    27601 attactcaag tttattttat tggagtttgt aaatgccaat cagttacctt ctggtactga
    27661 tgagcaacat ttaacatttg tgcctcttta aaatagtttc caataatttg tagtccaatt
    27721 ggtctacttt tcttcccaaa tccgataggt attgacatac caggtaaccc tgccaaattc
    27781 acagaacttg tatatatgtc cgataaatac atttgaatgg gatcatcaca tttttctccc
    27841 agatcaaatg caacggtagg tgttgtcgga cccatcagta catcacactg cttaaaaaca
    27901 tctgtaaaat cattcgcaat caagcgccgc aacttctgag ccttaatata ataagcatca
    27961 taataaccat gtgaaagcac ataagtacca atcagaattc gacgtttaac ctcttggcca
    28021 aatccctgtg cccgggtttt tttatacata tcatccaggt ttatatattg ttcagcacga
    28081 tatccatatc gtaccccatc aaatcgcgac aaattactgg atgcctcagc cggcgcaatc
    28141 acataataaa caggaattga aagttttgcg tttggtaatg aaacttcaat gatgttagcg
    28201 cccagtttct gatattcatc aatcgcttgt ttaactgcat gcgctacctc gtcacttagg
    28261 ccttgttcaa aatattcttt aggtaagcca atacgcagtc cggataaggg tctttctaaa
    28321 tcacgacagt aatcttcaac ctctcgctgc aaacttgtgg agtcgcgctc atcaaacccg
    28381 accatgacat ttagcatcat tgccaagtct tcggctgatc tagccatagg tccaccttga
    28441 tcaaggcttg aagcaaaagc aatcataccg aaacgagaaa caagtccata ggttggtttg
    28501 atgcctgaga taccacataa tgcagcaggc tgcctaatcg atccaccggt atccgttcct
    28561 gttgcagcag gcgccattcc ggccgcaaca gcacaagcag aaccaccaga actaccacct
    28621 ggaacggcat gaatatccca tggattctta actggtccaa agaaagaggt ttcattactc
    28681 gaacccattg caaactcatc catgttagtc ttgccaatat ttacagcacc aacctgatta
    28741 aagcgctcaa tcaccgtagc atcatagggc
//
LOCUS       DAOQKQ010000007        27948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_687_length_27948_cov_5.887140, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000007 DAOQKQ010000000
VERSION     DAOQKQ010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 27948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27948
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_687_length_27948_cov_5.887140"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /gene="fabF"
                     /locus_tag="PKZ49_00875"
     CDS             <1..996
                     /gene="fabF"
                     /locus_tag="PKZ49_00875"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HND35142.1"
                     /translation="QAIRDAGIEKIDGLNPERIGINIGSGIGGLTMIEDTDSAYHAGG
                     PRKISPFFIPSTIINMIAGNLSIMFGYQGPNLAIVTACTTSTHSIGSSARMIEYGDAD
                     VMVCGGAESCVTPLAIGGFSSARALSVRNSEPELASRPWDKGRDGFVLSEGAGVIVLE
                     EIEHARRRNAKIYAELAGFGMSADAYHMTAPCDDGSGASRCMLNGLNNAGINPDEVDY
                     INAHGTSTQLGDIAETTAIKRCFADHARKLAINSTKSMTGHLLGAAGGVEAIFSILSL
                     YNQVVPPTINLIEPDPDCDLDYVPNTARDMKMNVAVSNSFGFGGTNGTLVFRKFQ"
     gene            1009..2019
                     /gene="mltG"
                     /locus_tag="PKZ49_00880"
     CDS             1009..2019
                     /gene="mltG"
                     /locus_tag="PKZ49_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HND35143.1"
                     /translation="MPWQKQIVNKFKFLIIFFILGISLVTSWLYYLAHAAYVLPYKPY
                     EFSVEPGSSLKKIAVKLAEEKVIPSAWLFLLLSRITEQETAIKAGDYLLNRDISPLQL
                     LEYLAKGDSQRNEIRFIEGWTFAQIRETLNNHSGIKHDTLHLSEAEIATLLGANQKNL
                     EGLLFPDTYFFMKNSSDLGIIKRSYDKMQNHLQVAWSSKKESLPIKNEYEGLILASII
                     EKETGKDSDRELIAAVFINRLRLGMKLQTDPTVIYGLGDKFDGNLRKQDLVTDHAYNT
                     YTRLGLPPTPIAMPGLASIQAALNPAENNLLYFVAKGNGESHFSENLKEHNMAVQKYQ
                     KR"
     gene            2068..4683
                     /locus_tag="PKZ49_00885"
     CDS             2068..4683
                     /locus_tag="PKZ49_00885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HND35144.1"
                     /translation="MQFSVIIPTLNESENIDTLLTRLFALQFSSENYEIIFVDDGSTD
                     HTQEKIKQWSSQYTNVHLIERKGKPDLTASILAGVSAAKSDVIAVMDADLSHLPEQLP
                     LILEPVLTDRYDVAVGSRYVPGGSTKGWPLYRQWLSRAGGWLARPICDVNDATSGFFA
                     FKRNLISNIPEMARGYKILLELLVSNQEKIRITEVPICFHNRTQGSSKLSISHQVIFL
                     HRLIMLAGGIVSVGTASRFAMVGFLGVFVDAAVFHWLISSGAGLALAHITSFLAAVTV
                     NYTLNSKWSFRAHNGGRLDWYQFSRYLIVGLFALLLRGGVLALLVYTWDIPNGWAIFP
                     AIAATAIVNYLGSAFYVFPEKRTTLSLDTRWRVAAVGVVLFVILLRLVYIGLAQLLPD
                     ETYYWQYSQHMDLSFFDHPPLVAWLIWIGTAIAGQNEFGVRLGAFICGLVTLIYLYGF
                     ARNLYNKSVGMRAVMLFSVLPFGFASGLIMTPDAPLIAAWAATLFYMERALIAGQKSA
                     WLGMGIAFGLGLLSKYTLGLLGIAALVFVILDSTARRWMIRPYPYLSALLAFVLFLPV
                     VVWNAENNWVSFLFQTERIGGGGHVFSVHYLILYIAVILTPVGLFSAAHALARAPYDK
                     NSDFERRRRLFIYVFTGIPLAIFLGLSTYDTPRFHWTGPLWLAIIPAMAWVMGQNTLD
                     KFFNGLRNAWQSTIVVSLLAYAFILHYVVLGFPGVPYQFLTEHYFWKEATIEVEQIAE
                     EIKTETGQTPIVVGMSKWSVASALSFYNQNGNRMDIRSRNMLGDTGAMYEYWFPSEPP
                     TDRPILLVGMNRYALERSRTGNQFDHMLNHPSSIQHRVVTRHGIPVRPLYYRIAKGYL
                     GSATPYFAKNATSSP"
     gene            4793..5911
                     /locus_tag="PKZ49_00890"
     CDS             4793..5911
                     /locus_tag="PKZ49_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HND35145.1"
                     /translation="MILVLKPNTLLESLEYKQLLAHLGNLSGISTRIHSETGIEQTLT
                     EIYLIGNTKVLSIEDIQCFPCVDRVVRVSEEYRVLGRHQNDDRPTYFEYNGVRFGQDT
                     LNVFAGLCAVDTLEHVELMMKALRDHGQVCTRMGAYKPRTSPYSFQGHGKSCLPYVFD
                     LAGKYGIKVIAMEVTHESHVEEIREALYQTGSSAGVMLQIGTRNTQNFELLKIVGRQQ
                     DFPVLIKRGFGITLDESLNAAEYLASEGNRKVVFGLRGMKTNMGDPYRNFVDFAHVPV
                     VKRLTRMPVCIDPSHSVGMRSIAPDRILDIMHVTAQGVIAGANMVLADFHPVPSKALV
                     DGPQALLIKELPFFLEDIQIAREAYEKRVRLATRYLEV"
     gene            5996..6847
                     /locus_tag="PKZ49_00895"
     CDS             5996..6847
                     /locus_tag="PKZ49_00895"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HND35146.1"
                     /translation="MGLVVADTQGNRSDSLNLANRSNNIDFNAKRIISLSPAITELVF
                     SAGAGGKLVGVSRFSNFPSEAQLIPEIGDAYNLDLEKIIEISPELIIAWKQASIHKDL
                     DRLNNLGFQILEIEIKSLDDIPNLIRLIGKLAETAERAALAASQYEKTLLQMKQNYDN
                     RTLISVLQLIWLQPLITFGKDHLINEILGVCGGENVANFYPGSTFALATENIVAANPE
                     VIFDSTATMPEVRDLFKKLSSISAVKNDYIYFIPPDLIFRETVRALDAIQIACDYFDE
                     VREKMYQ"
     gene            6946..7917
                     /gene="ispH"
                     /locus_tag="PKZ49_00900"
                     /gene_synonym="lytB"
     CDS             6946..7917
                     /gene="ispH"
                     /locus_tag="PKZ49_00900"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HND35147.1"
                     /translation="MIKVLLANPRGFCAGVDRAIEIVERALNMHGAPIYVRHEVVHNR
                     FVVEDLEKKGAVFVENLEEVPQESILIFSAHGVSHAVRREAAARKLKIFDATCPLVTK
                     VHVEVAKMRKEGKEIVMIGHHGHPEVEGTMGQVEGNDKGMYLVETEDDVANLQVRDEK
                     NLTYVTQTTLSVDDAARVIDALKKRFPAITGPKKDDICYATQNRQDAVKKMVRNCDLV
                     IVVGSPNSSNSNRLCEVARNANVEAYMVDRAEQLQESWLIGKKCIGITAGASAPEILV
                     QQVLARLKQIGSNQTGQEVSVEELSGVVESVVFPLPKAEAISLEKHF"
     gene            complement(7892..9013)
                     /gene="thiO"
                     /locus_tag="PKZ49_00905"
     CDS             complement(7892..9013)
                     /gene="thiO"
                     /locus_tag="PKZ49_00905"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HND35148.1"
                     /translation="MSQFLLIGAGIIGLTSAVHLLKEGFSVTVLEKGEVGQESSWAGG
                     GILSPLAPWDYSEPVTQLVGYSTSIYSKWVDELSLSTEIDPEFNECGMLVLPPYDSVK
                     AHQWCISHNIKEEINHKSFWSPIHQSALEEAIFLPRVAQIRNPRLLAALQCEVIKRGG
                     VIKEFCEVSRLNLKNKSVVSISTTSGNYSADGYIITAGAWSRKILGKYALNLNIIPIQ
                     GQMLLYKFDAPPIPSILLQKELYLIPRLDGHLLIGSTMENVGFNKQITQAAYDKLYKK
                     ASRLLPEIETKHLIKHWAGLRPASPFNIPTIGRHPELDNLFINSGHFRYGVTMAPGSA
                     EILLNEIMGKPQPFDISPYQRGWHLPEKFNFSKNASLMK"
     gene            9125..9646
                     /locus_tag="PKZ49_00910"
     CDS             9125..9646
                     /locus_tag="PKZ49_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HND35149.1"
                     /translation="MHLIVNGGFTTLELMITLSILAILASVAVPSYQGMMVQSRLTTQ
                     TNELVTTLHFTRSEAIKRGSRVTICKSNNGASCTTASNWHDGWIVFTDTGVSGTVDGA
                     DQVLRVFPALKGSTLMSGGNFQNWLSYMPNGRALSGNFQNDTFRFCASAKRRDVVING
                     TGRPQIDKSVTTC"
     gene            9640..10083
                     /gene="pilV"
                     /locus_tag="PKZ49_00915"
     CDS             9640..10083
                     /gene="pilV"
                     /locus_tag="PKZ49_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HND35150.1"
                     /translation="MLSTNIKNQIATQKGVTMIEVLVSVIVLSIGLLGLAGLQSAGLT
                     HNQSASFRSTATMMTYSILDSMRANRTEASNGNYNIALGAAKPTGDATYQKDLNNWMT
                     ELALRLPTGLGSVNVDNNNNVTITIQWDDSRGSMAAQQFMMATRL"
     gene            10091..11128
                     /locus_tag="PKZ49_00920"
     CDS             10091..11128
                     /locus_tag="PKZ49_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HND35151.1"
                     /translation="MKKVINQQYGFTLIELMVAMTIGLLLLGGVITILSSSHQTYRVN
                     DALSRLQENARYAFQLLSRDIRMAGYLGCVGDGVPIVNTLNNPSDFLWKLGQPIEGFE
                     ASSSSAWTPALPSPSGTIPSPLGGRDIVVIRGVDGTDVKVIQHPGGNPPGSADLKVTV
                     GSGLQEDDIVLVTDCLAAAVFQVTNVNTSAGQDNVVHNTGNPTANTPGNLTKALGKEF
                     TGGELIKISTRSYFIRMNSNPTPFPSLYRKVGAGNAEELVEGVEDMQIEYGEDLDGNS
                     TADVYRTADAVTNWAEVVSVRISLLMQSLENNVATDAQTYTFNGTITTPNDRRLRQVF
                     TTVVTLRNRTS"
     gene            11174..11668
                     /locus_tag="PKZ49_00925"
     CDS             11174..11668
                     /locus_tag="PKZ49_00925"
                     /inference="COORDINATES: protein motif:HMM:NF025061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HND35152.1"
                     /translation="MTNQKGVVLITGLVFLVMMTILGVTAMQNTVLEERMAGNLRDEN
                     LAFQAAEAALREGERFLEQITLPTFNGSDGLYHHASSPAPDPMTWTGWTTSGKAISTS
                     ITGAASQPRYIIEQLVSVPFQGNKGSAQQSPVTNMSNMYRVIARGVGGTDSAVVVLQS
                     IYRR"
     gene            11687..15073
                     /locus_tag="PKZ49_00930"
     CDS             11687..15073
                     /locus_tag="PKZ49_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HND35153.1"
                     /translation="MKNQVFTGFIIVLLISLLIPYRAHATLALSDVPLFLTTAVDSNI
                     MFTLDDSGSMQFEIMPEEKIYGGVVYMFPRAESIYSGNVYSNASVDFDSNNPYAVALR
                     SSYVNKIYYDPAIRYQPWSNADGSLMSNASITCAPHNPTNTGVGCRDLTTNNTETASW
                     LNSDASLSTSSKTFYPAVYYLYQSGAILSTTSYTKVEIKSSIATYTGSVDRTDCASAP
                     TCSYAEEIQNFANWYTYYRSRILLSRAGIGKAFAAQGSNMRVGFGAINKGNTSIDGVM
                     AYTIVRGVRPFTSSNKSTFFSDLYNHPIYTYGTPLRWAIDDVGRYFQRTDDKGPWGET
                     PGSSGGTQYVCRQNYHILMTDGYWNDGFSGVGNADNTEGSSITNDTPNAAPATYKYTP
                     TLPYSDSFSDTLADVAMYYWKTDLRTDLANKVPTNGKDPAFWQHMVNFTVGLGVSGTL
                     TELPSGAGSWPDPTTSNGAKIDDLWHAAVNSRGEFFSAQNPTAFATGLSQVLNNISAR
                     TSSAAAVTTNTSRLNSGTRIYQAKFNSSDWSGQLLSYNLNSDGSIGTVQWEASAVIPN
                     HSSRKIFTHNGTNGIAFTTSNFSLLTSSQQNALNTNIGGVNDGQGVNRVAWLRGDKST
                     ELAQGGSFRNRGAGILGDIINSDLFFVRSLNFSYDGLVSGTPGQTTYYNYVQANESRT
                     PVIYTGANDGMLHAFNADTGVELFSYVPSSVYSKLSSLTSPSYTHRYIVDGNAYAGDA
                     YIGATPSWRTILLGTTGGGGKGIFALDITDPTNFSASNVLWEFTDADLGYVHGQAKIA
                     ILNNGDWVAIISNGYNSTSDKAFLYIVNLQTGALIKKIATNSTTSNGLSAPALVDTNG
                     DKMIDYVYAGDLQGNVWKFDISHTNTSQWDVAYGSGGTKYPLFTARNSSNQVQPITSP
                     LEIGVHPNGGFFIYFGTGQFIASGDNTNTQVQTLYGIWDNGTRISETNRSTLQQQTIE
                     TQTTSSPFRRTVSTNAVNWASKRGWYMDLLKPPSSSNQGERSIVMPLLNFGRILFTTL
                     IPSSDPCASGGSNWVMLLNGETGGMLTAPHFDTDKNGLLNSSDQIIAGVGSDGVRSES
                     VAISAGNLLHLIAGTTTGSVETITVSGNPLTPRRSWRQIQ"
     gene            15141..15617
                     /locus_tag="PKZ49_00935"
     CDS             15141..15617
                     /locus_tag="PKZ49_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HND35154.1"
                     /translation="MNSIYRHCPNSFAKRHFLCSGFTLIELMIAVAVLGIIAAVALPS
                     YQDHVRRTNRAEAKSILLEMSQLLERNYTAVNRYDQKSDGTGFTLSTTQSPKTGTAKY
                     TIQFAAGSLTQNAYTLQAVPTGSMTGDACSTLTLTNTGVKGVGAGATLDAATCWQR"
     gene            complement(15655..16455)
                     /locus_tag="PKZ49_00940"
     CDS             complement(15655..16455)
                     /locus_tag="PKZ49_00940"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HND35155.1"
                     /translation="MDHDQPVFTHINPLTMPPRFYCNHPLTINETILLPSGVSHHAAH
                     VLRLKKNNQVILFNGQGGEFTGQIEQIDKNGVTVQIQQFHDINRESPVMTELVQAICT
                     NEKMDWIIQKAVEVGVNRIQPIMTNRCVVHLHGEKVKKRAEHWQQIIHSACEQCGKNK
                     IPELLPLVSYTDWLSQFKNNQADRSITSSTKLMLTPTAQIKLSELQPNNKDFFILLVG
                     PEGGLTLSEEKLAITTGFILIRLGQRTLRTESAGLAALASIQALWGDY"
     gene            16503..17069
                     /locus_tag="PKZ49_00945"
     CDS             16503..17069
                     /locus_tag="PKZ49_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HND35156.1"
                     /translation="MASLETPVCEFGWKAVDFDLLGVDGKRYSLSSSRGRSGLLVMFI
                     CNHCPYVKAIRDRIIRDIKELEAYGINSIAIMSNDPLDYPEDSFENMAKVAKEYNYPF
                     PYVWDESQEIAKRYGAVCTPDFFGFNDKLELQYRGRFDASRKEAAPLDAHRDLFEAMV
                     QVAKTGKGPMDQIPSIGCSIKWRTDGNA"
     gene            17062..17856
                     /locus_tag="PKZ49_00950"
     CDS             17062..17856
                     /locus_tag="PKZ49_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HND35157.1"
                     /translation="MLEQVITIVKRVAEKDVMPRFMQVSRQHKGDGSFYTEADLAAQA
                     SLVTELTKLYPVVVMGEEMSQQQQLQCWEDGAAGLWSIDPIDGTSNFYNGIPYFAISV
                     ALMQNGRGLLGVIYNPATKELFYAERGKGAFLNGVALPIRKSTASLQSAVANVDLKRL
                     SRKLAAKVAANPPYASQRNYGACALEWCYTAAGYFDLYLHGGQKPWDYAAGSLILEEA
                     GGHMCGFEHDDYWLQTPWQRSVIAALDLQLFEQWRDWVRDQYLLLK"
     gene            17918..19363
                     /gene="trkA"
                     /locus_tag="PKZ49_00955"
     CDS             17918..19363
                     /gene="trkA"
                     /locus_tag="PKZ49_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HND35158.1"
                     /translation="MKILILGAGRVGSTVAESLVSESNDITVVDIVSGQLALLQDRLD
                     LRVVVGNASYPSVLKRAGAEDADMILAVTESDEINLVSCKLAASLFNTPTKIARIRSA
                     EYLEHSEIFAKDNFCVDFAICPEQIVTEYIENLIEFPEALQVLDFAHGKISLVAVKAL
                     QGGPLVGQQLQELRRHVPNVDTRVAAIFRKDRPILPEGDTIIEVGDEVFFIAATEDIR
                     AVMAELRSMDKPAKRIMIAGGGKIGKRLAYVLEKNYQIRVIERDVKNCEYLAQELQDT
                     LVLQGDVTDEELLESENVAEMDVFCALTNDDENNIMASLLAKKMGARKVIALINRSIY
                     VDLVQSSGIDIAISPAQVTIGSLLAYVRQGDVAVVHSLRRGAAEALELIAHGDANSSH
                     VVGKKIEEVQLPKGATIGAIVRGLPPGEERWENKAGDKENDSGSRNKAKVIIAHHDTV
                     IESEDHVIVFAINKKMIRQIEKLFQVSASFL"
     gene            19377..20834
                     /locus_tag="PKZ49_00960"
     CDS             19377..20834
                     /locus_tag="PKZ49_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HND35159.1"
                     /translation="MSHFLSVANLLGKIIAVFALTMLIPYGIAIWSDDGSSTIFGDSI
                     LVTFISGIVISLITLPYKKELQIRDGFLLVVLVWLLLPCFGMLPLLNYFPELSIAKAY
                     YEATSGLTATGGTVLTGLDTLPYSINLWRGEMVWLGGMGLIVLAVAILPMLGVGGRQL
                     FKAEIPGPMKEAKLTPRIAETAKGLWLVYLVLTIACLIAYKLAGMNWFDAIMHAFTTL
                     GLGGFSSHDASYGYWNSPLIEFVAMVFMLISGINYATHFLVWRTNSLSSYQNDPEIRP
                     YIYIVLSSCIFLAVYLWLMGVYDDPLHALRYAAFNTISVATTTGYSNTDYSLWPIFAP
                     LWMLFLSGFCTSSGSTGGGIKMIRVRILYRQFFREMITIMHPQAISPVKLGRNIISNQ
                     IIFAVLVFLFVYLSSIILITLLLTLSGLDEMTAFSAAVASFNNLGPGLNQIGPATTYA
                     SLSDFQIWICSFSMLLGRLEFYTLLIVFTAAFWRK"
     gene            20955..21368
                     /locus_tag="PKZ49_00965"
     CDS             20955..21368
                     /locus_tag="PKZ49_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HND35160.1"
                     /translation="MTEATSGNDLVGKKIMIVDDSNTIRRSAEIFLAQSGCEVILATD
                     GFDAMAKIIDHQPDVIFLDIVMPRLDGYQACMLIKKNPRFHSVPVIMLSSKSGLFDRA
                     RGRMVGSDEYLTKPFTKEGLLNTIKKHTQQQNKVA"
     gene            21407..21790
                     /gene="apaG"
                     /locus_tag="PKZ49_00970"
     CDS             21407..21790
                     /gene="apaG"
                     /locus_tag="PKZ49_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HND35161.1"
                     /translation="MDNEKKYDIDIVVRTAYLSEQSDEEAGRHVFAYTITISNTGSIA
                     SQLISRHWIITSGDGNVHEVRGLGVVGEQPLLKPGDSFEYTSGTAISAFAGSMKGSYQ
                     MVAEDGVHFSVEIPEFILSVPRVLH"
     gene            21873..22589
                     /locus_tag="PKZ49_00975"
     CDS             21873..22589
                     /locus_tag="PKZ49_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HND35162.1"
                     /translation="MQYGRLFLIPSPLGEGAIDWIIPSHVKQCIANLTYFVVEHPKTA
                     RHFLKQIALQRPIQAITLQTLDKHTPINELDSLLGPIYAGNDMGLLSEAGCPAIADPG
                     ANLVRIAHEKQIQVVPLVGPSSILLALMASGLNGQCFSFHGYLPIDKVSCAKKIIQME
                     SESFKFNQTQIFIETPYRNKKLFELLAMHCQGSTHLCIASNLTLQDEVINTKTIKDWQ
                     TNLPDFEKKPTVFLLHRYQT"
     gene            complement(22651..23040)
                     /gene="gloA"
                     /locus_tag="PKZ49_00980"
     CDS             complement(22651..23040)
                     /gene="gloA"
                     /locus_tag="PKZ49_00980"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HND35163.1"
                     /translation="MRILHTMLRVGNLDNSLQFYTQVLGMKLLRQKDYPEGKFTLAFV
                     GYQHESEGAVLELTHNWDTEKYDIGTGYGHIAIEVADAYQACDSVRKLGGRVIREAGP
                     MKHGTTVIAFVEDPDGYKIEFIQKKNE"
     gene            complement(23040..23228)
                     /locus_tag="PKZ49_00985"
     CDS             complement(23040..23228)
                     /locus_tag="PKZ49_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HND35164.1"
                     /translation="MSTDQNPVYHRYMCLICGYIYDEAEGSPQEGIPPGTRWEDVPIN
                     WTCPECGARKDDFEMVKL"
     gene            23281..24108
                     /locus_tag="PKZ49_00990"
     CDS             23281..24108
                     /locus_tag="PKZ49_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HND35165.1"
                     /translation="MLQPPPIVLSFAASDPSGGAGIQADILTLASMGCHPLSVVTAIT
                     VQDTVGVESFLPLESEWVADQARIVLEDMPVHAFKIGVLGSVEAIVTIAEVVADYPNI
                     PLVLDPVLASGRGDSFANDEILQAMRDLLFPQTTIITPNSLEARRLMQENEEDPEIVD
                     LSQSAKSFLRLGCQYVLITGTHENTSKVINTLYDKNGVVRVDEWQRLPGSFHGSGCTL
                     SSAISAALAHGMSVSEGVYEAQDYTWNTLKSGFRPGMGQYLPDRFFWASNEGESDQK"
     gene            24116..24739
                     /gene="thiE"
                     /locus_tag="PKZ49_00995"
     CDS             24116..24739
                     /gene="thiE"
                     /locus_tag="PKZ49_00995"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HND35166.1"
                     /translation="MVQRSRIEGLYAITPDIENTSDLVEKVDQVLSSGIQYIQYRNKS
                     IAHEQRVNQACAIKKRCQRYGAKLIINDHLDIALAVNADGLHVGKVDVSIQTARTYLG
                     KHKMIGVSCYNQIDLALAAEKEGADYVAFGAFYASKTKIDTVEANPDLLIQAKKVLQI
                     PIVAIGGINVTNAALLIFRGCDAIAVSDGLFNTANIPSIAKKFKRLF"
     gene            24776..26056
                     /gene="hemL"
                     /locus_tag="PKZ49_01000"
     CDS             24776..26056
                     /gene="hemL"
                     /locus_tag="PKZ49_01000"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HND35167.1"
                     /translation="MNISNQQLYDLSQQYIPGGVNSPVRAFKSVGGTPIFFQRGEGAY
                     FWDVEGKCYIDYVGSWGPLILGHAHPQVVKAVQETAINGLSFGAPTKAELDLAELLCK
                     LLPSLEQVRLVSSGTEAGMSVIRLARGYTGRSHIIKFEGCYHGHDDALLVKAGSGALT
                     FGHPSSAGVPAETAAHTLVLNFNDVEGLKRTFTELGEQIAAVIVEPVAGNMNLVKATP
                     AFLSELRSLCSRYGSVLILDEVMTGFRVGLTCAQGMYQIQPDLTVLGKVIGGGMPLAA
                     FGGKREIMQCLAPVGGVYQAGTLSGNPVSVAAGLATLREIQAEGFYSTLAVRTTQLTD
                     GLTAMARKHNVAFCAQAVGGMFGLYFSSKLPQSFSEVMACDKEAFNRFFHAMLKEGIY
                     FAPSAFEAGFVSSAHSEDDLSKTLAAADRVFSSW"
     gene            complement(26155..26799)
                     /locus_tag="PKZ49_01005"
     CDS             complement(26155..26799)
                     /locus_tag="PKZ49_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HND35168.1"
                     /translation="MKVIKNIVIITALIFSNQILADATKTVQGDAEKGKEIASGVCAG
                     CHNADGNSVIPTNPTLAGQHAEYLLKQLKDFKMEEDTPAKRDNPIMSSMVAPLSVDDM
                     QDLAVFYAQQKTQPANNNVDESQLELGKRVYNGGNLENSVPACSSCHSPNGAGIPPLF
                     PKLAGQHADYTLSQLETFRQGTRANDINNTMQMIVLRLSAQEKKAVAEYISTLK"
     gene            26915..27517
                     /locus_tag="PKZ49_01010"
     CDS             26915..27517
                     /locus_tag="PKZ49_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35169.1"
                     /translation="MKIFFYLLLFINIITLVIIAIVELNSNKNNRVMEFHAEKIRILP
                     AQVACLEWLSLYGVALQQARTELPKLNLDHFVIESPMDEITVYIIHIAALKTKREVDR
                     QLAQLQKLNIAYQLIQENVGYLQKDSIMLGMYQERSAAMRKLEELSSMNVNNAIISER
                     DIEQVKYIFLEPTEDVRIYLQQLVEKFPDSYLKLTQCDRF"
     gene            27572..>27948
                     /locus_tag="PKZ49_01015"
     CDS             27572..>27948
                     /locus_tag="PKZ49_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HND35170.1"
                     /translation="MAIQIKTPKQVEIMRIAGRLAAEVLDHITPHVLPGVTTGELDEI
                     CHHYMVNVQKTIPAPLNYAPPGYSPFPKSICTSVNHQICHGIPGAKKLKNGDIVNVDV
                     TVIYDGYHGDTSRMYYVGEPSIQA"
BASE COUNT         7956 a         4990 c         6393 g         8609 t
ORIGIN      
        1 caggcgatta gagatgcagg tattgaaaaa atcgatgggt taaatccaga gcgtatcggt
       61 attaatattg gttctggtat tggtggactt acaatgattg aggacactga tagtgcctac
      121 catgctggag gcccccgtaa aatatcgcct ttttttatac ccagtacgat tattaatatg
      181 atagcgggta atttatcgat tatgtttggt tatcaaggac cgaacttggc gattgtaact
      241 gcttgtacta cttctactca tagtattggt agttctgcac gaatgattga atatggtgat
      301 gcggatgtta tggtatgtgg tggcgctgaa tcttgcgtaa ctcctcttgc aatcggtgga
      361 ttctcatcgg cacgtgcttt gtcagtcaga aattcagaac ccgaattagc aagtcgtccc
      421 tgggataaag gtagagatgg tttcgtattg agtgagggcg ctggcgtaat cgtactagaa
      481 gaaatagagc atgctaggcg acgtaacgct aaaatttatg ctgagttagc tggatttgga
      541 atgagcgcag atgcctatca tatgactgct ccgtgtgatg atggtagtgg tgcttctcga
      601 tgtatgctaa atgggctcaa taacgcgggt atcaatccag atgaagttga ttatattaat
      661 gcccatggta cttctactca attaggtgat attgcggaaa cgactgcaat caaacgttgc
      721 tttgctgatc acgctaggaa attagcgatc aattctacta aatcaatgac tggccactta
      781 cttggcgcag cagggggagt agaagctatt ttctcgattc tgtcgctata taatcaggtt
      841 gtacctccaa ctataaatct aatcgaacca gatccagatt gcgatctaga ctatgttcct
      901 aacacagctc gagatatgaa aatgaatgtt gcagtttcca actcgtttgg gtttggcgga
      961 acaaatggta cattggtatt tagaaaattt cagtagatta ctttgcatat gccatggcag
     1021 aagcaaatcg ttaataaatt caagtttctc atcatttttt ttattcttgg tatttctctt
     1081 gttactagct ggctctatta tctagcacat gcagcatatg tattgcccta taaaccttat
     1141 gaattttcag ttgaacccgg cagcagttta aaaaaaatcg cggtaaagct tgctgaagaa
     1201 aaggtaatac cgagtgcttg gttattcttg ctattatcaa gaataacaga gcaagaaaca
     1261 gcgattaaag cgggcgacta tttgctaaat agggacattt caccacttca gctcctggaa
     1321 taccttgcca aaggcgatag ccaacgtaat gaaattaggt ttatcgaagg gtggactttc
     1381 gctcaaattc gggaaacact caataatcac tcagggatta agcatgatac gttgcacttg
     1441 agtgaagcag agattgcaac gttacttggc gcaaatcaaa aaaacttaga aggcttgtta
     1501 tttcccgata cttatttctt tatgaaaaat agtagtgatc taggtattat aaaacgatcc
     1561 tatgataaga tgcagaatca tctccaggtt gcttggtcgt caaaaaaaga atcgctaccg
     1621 attaaaaatg aatatgaggg cttgattctt gcttcaatca tcgaaaaaga aacaggaaaa
     1681 gatagtgatc gagaattgat agcagcagta tttatcaatc gcctgcgcct cgggatgaaa
     1741 ctacaaaccg atccaacagt aatatatggg ttaggtgaca agtttgatgg aaatttgcga
     1801 aagcaggatc tagttactga ccatgcctac aacacttata ctcgattagg tttaccccca
     1861 actcccattg caatgcctgg tcttgcttct atccaagcag cgcttaatcc ggctgaaaat
     1921 aatttgctct atttcgtggc taaagggaat ggcgaatcac atttctcaga aaacctaaaa
     1981 gaacataata tggctgtaca aaaataccag aagcgataat tgattgtttc ttatgaataa
     2041 tataataaat aaggatgaat aaacctgatg caattctcgg ttattattcc tacacttaac
     2101 gagtcagaaa atattgatac attattgacg cgtttgtttg cactccaatt ttcttcagaa
     2161 aactatgaaa ttatttttgt ggatgatggc tcaaccgatc atacacaaga aaaaattaag
     2221 caatggtcat cacaatatac taatgttcat cttatagaaa gaaaaggaaa gcctgattta
     2281 accgcctcga ttcttgcagg ggtatccgca gcaaaaagtg atgtgattgc tgttatggat
     2341 gcagatttga gtcacctgcc tgagcaacta ccgttaattt tagagccagt attaactgat
     2401 cgatatgatg ttgcggttgg aagtcgatat gtcccaggag gaagtaccaa gggatggcct
     2461 ttatatcgac aatggctttc tcgtgctggc ggttggttag cgcgtcctat ttgtgatgtt
     2521 aatgatgcaa catctggttt ttttgcattt aaacgtaact tgatcagtaa tattccagag
     2581 atggcgcgtg gctataaaat tcttctggaa ttattagtat caaatcaaga aaaaattcga
     2641 attacggaag taccgatttg ttttcataac agaacacaag gtagctctaa actttcaatt
     2701 tctcatcaag tcattttttt acatcgactg attatgttag ccggtggaat tgtatctgtt
     2761 ggaactgcaa gtcgctttgc aatggtggga tttttaggag tgtttgttga tgcggcggtt
     2821 tttcactggc tgataagttc aggcgctggg ttagctttgg cccatatcac tagctttcta
     2881 gcagctgtga ctgttaatta tacattgaat tctaagtggt cattccgagc acataacgga
     2941 ggtcgattag attggtatca gtttagtcgt tacctgatcg ttggactttt tgctttgttg
     3001 ctaaggggag gagtacttgc gctccttgtg tatacctggg atatacccaa tggatgggca
     3061 atatttcctg caattgctgc gactgcaatt gtaaattatc ttggttcggc tttttatgtt
     3121 tttcctgaaa aaagaacaac actatcactg gatactcgct ggagagttgc agcagtcggg
     3181 gttgttcttt ttgtgatatt gttgcgcctt gtttatattg gattggctca attgctgccg
     3241 gatgagactt actattggca atattcccag catatggatc taagtttttt tgatcatcca
     3301 cccttagtcg catggttaat ttggattgga actgctatag caggtcagaa tgaatttgga
     3361 gtgaggttgg gtgcgttcat atgtggttta gtgacgctca tatatctcta tggtttcgca
     3421 cgtaacctgt ataacaaatc agttggtatg cgagccgtaa tgcttttctc tgttttacca
     3481 tttggctttg catccggttt aattatgacg ccagatgcac ctctgattgc tgcctgggct
     3541 gctactttat tttatatgga gcgggcattg attgcgggtc agaaaagtgc atggcttggc
     3601 atgggaattg cttttggctt gggtttgtta tcgaaataca cgctcgggtt actcggtatt
     3661 gccgcgttag tttttgttat tttggattcc accgcgcgcc gttggatgat tcggccatat
     3721 ccctatttat ctgcgctgct cgcttttgtt ctctttttgc ctgtggtcgt atggaacgct
     3781 gagaacaact gggtttcgtt tttgtttcaa actgaaagaa tcgggggtgg tggccatgtg
     3841 ttttctgttc attacttaat tttatatatt gcagttatat tgacaccggt aggtttgttt
     3901 tcagctgctc atgcattagc tcgtgctcca tatgacaaaa atagtgattt tgagcgtagg
     3961 cgtcggttat ttatttatgt ttttacaggt attccactcg ccatcttctt aggtcttagc
     4021 acctatgata cgcctcgatt tcactggacg gggccgctct ggctagcaat aattcctgcg
     4081 atggcctggg tgatgggaca aaatacactt gataaatttt ttaatggcct acgaaatgca
     4141 tggcaatcta ccatcgttgt atcattgctg gcctacgctt tcattttgca ttatgtcgta
     4201 ctgggttttc ctggggtacc ctaccaattc ttaacagaac attatttctg gaaagaagca
     4261 acgattgaag ttgagcaaat tgccgaagaa atcaaaactg agactgggca aacaccaatt
     4321 gtagtaggga tgagtaaatg gtcggtcgcg agcgcgctat ctttctataa tcaaaacggc
     4381 aataggatgg atatacgttc acgtaacatg cttggggata ctggagcaat gtatgaatac
     4441 tggtttccaa gcgagccacc cacagatcgc cctatactat tggtgggaat gaatcgctat
     4501 gcacttgagc gaagtcggac tggaaatcag tttgatcata tgctaaacca tcctagttca
     4561 atacaacacc gtgtggttac tcggcatggt attccggtaa gacctttgta ttatcgaatt
     4621 gctaaaggtt atctcggtag cgcaactcca tattttgcaa agaatgcaac tagttctcca
     4681 taaaataaac attttcagct taatagaaat ggtaataaac tattcctaga tctaaagcca
     4741 gctggaagcg atttaccttg aagattacta tttattgcta ttaggcattt ttatgattct
     4801 tgttttaaag ccaaatactt tacttgagag cttggaatat aagcagctac ttgcgcatct
     4861 gggaaatttg tctggtattt ctactcgaat tcattctgaa actggtattg aacaaacgct
     4921 aacagagatt tacttgatcg gtaataccaa agtattatcg atagaagaca ttcaatgttt
     4981 cccttgtgtc gatcgtgtgg tcagggtctc agaagaatat cgcgtgttag ggaggcacca
     5041 aaatgatgat cgtccaacct attttgagta taacggggtg cgctttggtc aagacacatt
     5101 gaatgtgttt gcagggcttt gtgcagttga tacgttagag catgtagagc tcatgatgaa
     5161 agcgttgaga gaccatggtc aggtatgtac tagaatggga gcctataaac cacgaacaag
     5221 tccttactca tttcaaggtc atggtaaatc ttgtttgcct tatgtgttcg atcttgctgg
     5281 taagtatggt attaaagtta tcgcaatgga agtgacgcac gagtctcatg ttgaggaaat
     5341 tcgtgaagct ttatatcaga caggaagttc tgcaggggta atgctgcaga ttggaacgcg
     5401 taatacacaa aattttgaat tgttgaagat tgttggtcgt cagcaggatt ttccggttct
     5461 tattaaacgt ggttttggaa ttacattgga cgaatcacta aatgcagccg agtatcttgc
     5521 ttccgaaggt aatcgaaaag tggtttttgg tttgcgtgga atgaaaacga atatgggtga
     5581 tccataccgt aattttgtcg attttgcaca tgttcctgtt gtcaaacgtt tgacgagaat
     5641 gccggtttgt attgatccat cacattcagt cggtatgcgt tcaattgcgc cggatagaat
     5701 tttggatatt atgcatgtga ctgcacaagg tgttattgct ggcgctaata tggtactggc
     5761 tgattttcat cctgttccaa gtaaagcgct cgttgatgga cctcaagcat tattgatcaa
     5821 agagttgccg tttttcctag aggatattca aatcgcgcgt gaagcttatg aaaaaagggt
     5881 aagactcgct acccgttatc tggaagtgta attgaattga tctttatcgg tgtataactt
     5941 tgagtttagc tgattgagaa catactcaat ttgtttactt ctcctatttt ttgctgtggg
     6001 acttgttgtc gctgatactc aaggaaacag aagcgattcc ctaaatctcg ctaaccgttc
     6061 taataatatt gattttaatg ctaagagaat tatctcactt tctcccgcaa ttactgaatt
     6121 agttttttca gctggggctg gaggtaagct agtaggcgta agtagattta gtaattttcc
     6181 tagcgaagct caattgattc ctgaaatcgg ggatgcatat aacctcgatc ttgagaaaat
     6241 tatcgaaatt agccctgaac tcattattgc ttggaagcag gcttcgatcc acaaagatct
     6301 tgatcgattg aataatcttg gttttcagat acttgagatt gagatcaaga gcctggatga
     6361 tatacccaat ttaataaggt taattggtaa actggcagag acagcagaac gcgcagcatt
     6421 agcggcgagt caatatgaga aaactttgct tcaaatgaag cagaattatg ataatagaac
     6481 gctaataagt gtccttcaat taatttggtt acaaccgcta ataacgtttg gaaaggatca
     6541 tctaattaac gagatattag gggtatgtgg tggggaaaat gtagctaatt tttatccagg
     6601 tagtacattt gcactcgcta cagaaaatat tgtagcagca aatcccgaag tgatttttga
     6661 tagtacagct actatgccag aggtacggga tctttttaaa aagctttcat cgattagtgc
     6721 agtgaaaaac gattatatct actttattcc acctgattta atttttaggg aaactgtacg
     6781 agctctcgat gccattcaaa tagcttgtga ttattttgat gaagttcgag aaaaaatgta
     6841 tcagtgaagg caacgtataa tatcttataa tgcgagtaat gttcataata aataaaaaag
     6901 ttagtttttc taactaaagc taaatgcttt tatggagaag agaaaatgat taaagttttg
     6961 ctagctaatc ccagaggatt ttgcgcaggc gttgatcgcg cgatagagat tgtagaacgt
     7021 gcgcttaata tgcatggtgc tccaatttat gtgaggcatg aagtagtgca taaccgcttt
     7081 gttgttgagg atttggagaa aaaaggcgca gtgtttgttg agaatttaga agaagtgcca
     7141 caagagagta ttttgatctt tagcgcgcat ggtgtttcac atgcggttcg tcgtgaagca
     7201 gcagcacgaa aacttaagat ttttgacgca acttgcccac tcgttacaaa agttcatgtc
     7261 gaagtagcga aaatgcgtaa agaaggtaaa gagattgtta tgattgggca tcatgggcac
     7321 cctgaggtag aaggaacgat ggggcaagtg gaaggaaatg ataaaggaat gtatcttgtg
     7381 gaaacagaag atgatgttgc caaccttcaa gttagagacg aaaagaattt aacttatgtg
     7441 actcagacca ctttatctgt tgatgatgca gctcgtgtaa ttgatgcttt gaagaaaaga
     7501 tttccagcga ttactggccc aaagaaagat gatatttgtt acgcgactca gaatcgccaa
     7561 gatgcagtta aaaaaatggt acggaattgt gatttagtga ttgtggtcgg ttctcccaat
     7621 agctcaaact ctaatcgctt gtgtgaagtt gcgcggaatg ccaatgttga agcatatatg
     7681 gttgatcgag cggaacagct tcaagagtca tggcttatag gaaagaaatg tataggtatt
     7741 actgcggggg catctgctcc agaaatattg gttcagcaag ttttggctag gcttaagcag
     7801 attggctcta atcaaacggg tcaggaagtg agtgtcgaag agctaagtgg tgttgttgaa
     7861 tcagttgttt ttccgctacc taaagcggag gctatttcat tagagaagca tttttagaaa
     7921 aattgaattt ttcaggcaga tgccatcccc tttgataagg ggaaatatca aagggttgtg
     7981 gttttcccat tatttcgttt aagaggattt ctgcacttcc tggagccatc gttactccat
     8041 agcgaaaatg accgctgttg ataaaaaggt tatccaattc aggatgtcgc cctatggttg
     8101 gaatattaaa gggcgaggct ggccttaagc cagcccaatg tttgattagg tgcttagttt
     8161 ctatttctgg aagaagtcgt gatgcttttt tatataactt atcataggca gcctgggtta
     8221 tttgcttatt aaacccaaca ttttccattg tacttccaat caataagtgc ccatcaagtc
     8281 ttggaatcag atatagttct ttttggagga gaatagacgg gataggtggg gcatcaaact
     8341 tgtataatag catttgccct tgtatcggga tgatattgag atttaatgcg tattttccta
     8401 atatttttct actccatgct cctgcagtaa tgatgtagcc atctgcagaa taattaccac
     8461 tagttgtgct aatagataca acggacttat tttttagatt tagtctactt acttcacaaa
     8521 attctttaat cacgcctcct cgcttaatca cttcacattg aagtgcagct aataagcgtg
     8581 ggttgcgaat ttgagcaaca cgaggaagaa agatcgcttc ttctagagca gattggtgaa
     8641 ttggactcca gaaagattta tggtttattt cttccttgat attgtgtgaa atgcaccatt
     8701 gatgtgcttt tactgaatca tagggaggta gcactaacat gccgcattcg ttgaattcag
     8761 ggtctatttc tgtagataac gagagctcgt ccacccactt gctatatatg cttgtgctgt
     8821 agcctacaag ttgcgtaaca ggctcagaat aatcccacgg ggctaggggc gataggatgc
     8881 caccaccagc ccaagatgat tcttggccta cttctccttt ttccaaaact gtaacagaaa
     8941 atccctcctt aagtaggtgt actgctgatg taaggccaat aattccagca ccaataagca
     9001 agaattgtga cattttatta gatgattttt ccgttttatt aagtagatgt cattttttaa
     9061 tcaattaaag ttgatttttt aataaccgtt actttgtgtg gatgaagaat gaacaggaat
     9121 aataatgcat ttaattgtaa atggtggttt tacaacgttg gaactgatga taaccctcag
     9181 tatcctcgct atcctggctt ctgttgctgt gccaagctat cagggcatga tggttcaatc
     9241 acgcttgacg acccagacca atgaacttgt aacaacgctt cactttaccc gatctgaagc
     9301 aattaagcga ggttcaaggg tgacgatctg taaaagcaat aatggagcca gctgcacgac
     9361 agcgagcaat tggcacgatg gctggattgt atttactgat acgggtgttt caggaacggt
     9421 cgatggagca gatcaggtac ttcgagtttt tcctgcgctc aaaggaagta cactaatgag
     9481 tggtggaaat tttcagaatt ggctttctta tatgccgaat ggccgtgcgt tatcaggtaa
     9541 ttttcagaac gatacattta ggttctgtgc tagtgcgaaa aggcgcgatg ttgtaataaa
     9601 cggtacaggc cgtccgcaaa tagataaatc ggtaacgaca tgctaagtac caatataaaa
     9661 aatcaaatag ctacacaaaa gggtgtaacg atgatcgaag tgttggtatc agtcattgtt
     9721 ctttctattg gtttgcttgg ccttgctgga ctccaatccg caggacttac tcataaccag
     9781 agtgcgagct ttcgttcaac agcgacaatg atgacctata gcattcttga tagcatgcgt
     9841 gctaatcgaa ctgaggcgag taatggtaac tataacattg ccttgggagc agctaaacct
     9901 actggcgatg caacatacca gaaggatctc aataattgga tgactgaatt agcgttaaga
     9961 ttacctacag ggttgggatc agtaaatgtg gataacaata ataatgtgac gatcactatt
    10021 cagtgggatg acagtcgagg ctccatggct gctcagcaat tcatgatggc tacccggctc
    10081 tgaggtgcaa atgaaaaaag taatcaacca gcagtatgga tttacactaa tagagttaat
    10141 ggttgcaatg acgatcgggc tgttattgct gggtggtgtt attacaatac tatcgtctag
    10201 ccaccaaaca tatcgagtca atgacgcatt gtctcggcta caagaaaatg ctcgctatgc
    10261 attccagctt ctatcgcgtg atatccgcat ggcgggatac ttaggttgtg taggagatgg
    10321 agtacctatt gttaatactt taaacaatcc atcagatttt ctttggaagc tagggcaacc
    10381 catagaagga tttgaagcaa gtagttcttc ggcatggact cctgcattac catcaccatc
    10441 agggactatt cccagtcctc tgggtgggag agatattgtt gtgattcggg gggtagatgg
    10501 tacggatgtt aaagtgattc aacacccagg cggtaaccca cctggatccg ctgatttgaa
    10561 agttacggta ggcagcggcc ttcaagaaga cgatattgta cttgttacgg attgccttgc
    10621 agctgctgtt ttccaagtta ccaatgtaaa tacttctgcc gggcaagata atgttgttca
    10681 taacactgga aatccaaccg caaacacgcc tggtaacctt actaaagcgc tgggaaaaga
    10741 atttacgggt ggggagttga tcaaaatttc tacacgctct tattttatcc gaatgaattc
    10801 taatccaacg ccttttcctt cgctttatcg taaggtaggt gcaggtaatg ctgaagaatt
    10861 ggttgaaggt gttgaggata tgcaaatcga gtatggggaa gatttggatg gtaactcaac
    10921 agcagatgtt tatcgtacag cagatgcagt tacgaattgg gctgaagttg ttagtgttcg
    10981 aataagtctt ttgatgcagt cattggaaaa taatgtagct actgatgcgc aaacttatac
    11041 gtttaatgga accataacaa cacccaatga tcggagatta cgccaagtgt ttactaccgt
    11101 agttacattg cgcaatcgca cttcttaaaa cagattgttg aacatcatac aaatgagaaa
    11161 tcaagaaata tcaatgacta atcaaaaagg ggtagtgctt atcacgggtt tggtttttct
    11221 ggtcatgatg actattcttg gtgtaacggc gatgcaaaat accgttttag aggaaagaat
    11281 ggctggaaat cttcgtgatg aaaatcttgc gttccaagct gctgaggctg ctttaaggga
    11341 aggcgagcga ttcttggaac aaataacgct acctactttt aatggttctg atgggcttta
    11401 tcatcatgca tcgtctcctg cgcccgaccc aatgacatgg acaggttgga cgacttcagg
    11461 aaaagcaata agcacatcga ttacgggagc agcgagtcag ccacgctata ttattgaaca
    11521 gcttgtcagt gtcccctttc aggggaataa aggtagcgca caacaatcgc cagtaacaaa
    11581 tatgtctaat atgtatcgcg tgattgcaag gggagtcggt ggcacagatt cagcagtagt
    11641 cgtactgcaa agtatttatc gtcgataatt caaaggagtg gcttaattga agaatcaagt
    11701 ttttacggga tttattatcg tattgctaat ctccttgcta attccatatc gagctcatgc
    11761 aacgctggcg ttatcagatg tacctttatt tctgactaca gcggttgatt caaatattat
    11821 gtttacgctt gacgattctg gatccatgca gtttgaaatc atgccagagg agaaaatata
    11881 cggtggggtt gtttatatgt ttccacgtgc agagagtatt tatagtggga atgtttatag
    11941 taatgcttca gtagattttg attcaaataa cccttatgca gttgcattgc gttccagtta
    12001 cgttaataag atttattatg atccagcaat aaggtaccaa ccctggagta atgcagatgg
    12061 ctcactgatg agtaatgcat caattacctg tgcaccccat aatcccacta ataccggtgt
    12121 tggttgtagg gatttgacaa ccaataatac ggaaacagcc agttggttaa atagtgatgc
    12181 tagtctttca acctcatcta aaacttttta tcctgcggta tattatcttt atcagagcgg
    12241 ggcaatattg tcgacgacta gttatacgaa agtagaaatt aaatcttcga ttgcgactta
    12301 tacaggtagt gttgatcgca ctgattgtgc aagtgcaccc acttgtagtt atgcagaaga
    12361 gattcagaat tttgctaatt ggtatactta ttaccgctct cggatactgc tttcacgtgc
    12421 aggaattggc aaggcatttg ctgcacaagg gagtaatatg cgggtgggtt ttggcgcaat
    12481 aaataaaggt aataccagta tagatggggt aatggcttat accattgtta ggggcgttag
    12541 accatttact tctagtaata agtcaacttt ttttagcgat ctttataatc atccaattta
    12601 tacgtatggc actccacttc gatgggctat cgatgatgtt ggtagatatt ttcaaagaac
    12661 cgatgataaa ggtccttggg gagaaacgcc tggttcaagc ggtggaacac aatacgtgtg
    12721 tcgtcaaaat tatcatattt taatgactga tggttactgg aatgatgggt tttcaggagt
    12781 tggtaatgct gacaatacag agggtagctc aatcaccaac gatacaccca atgctgctcc
    12841 tgcaacttat aaatatactc caacgttacc ttattctgat agcttcagtg acacattggc
    12901 tgatgttgca atgtattatt ggaagactga cctgaggact gatttagcca ataaggttcc
    12961 tacaaacggt aaggatcctg ctttctggca acacatggtg aattttactg ttggactcgg
    13021 tgttagtgga actttaactg agctgccatc aggcgctgga agttggccag atccaacaac
    13081 tagtaatggt gccaaaatag acgatttatg gcatgcggcg gtaaatagtc gtggtgagtt
    13141 ttttagtgct caaaatccta ctgctttcgc aactggttta agtcaggttc taaataatat
    13201 ttcagctaga acgagctctg cagcagccgt aacaactaat acaagccggc ttaactcggg
    13261 tactcgtatc tatcaagcta agtttaatag cagtgattgg agtggtcagt tactatccta
    13321 taatttgaat tccgatggtt ccataggaac tgtgcaatgg gaagcgtccg cggttatacc
    13381 gaaccactca agtcgaaaaa tttttacgca taatggaacc aatggaattg cattcacaac
    13441 gagtaatttt tctttattaa ccagttctca gcagaatgct ttaaatacaa atatcggtgg
    13501 ggttaatgat gggcaaggtg ttaatcgagt tgcttggttg agaggagata agtcgacaga
    13561 attagcacaa gggggaagtt ttcgtaatcg cggtgcagga atacttggtg atatcatcaa
    13621 ttctgattta ttttttgtta ggtcattaaa ttttagctat gatggtctcg tttcgggaac
    13681 gccaggtcaa accacttatt ataattacgt tcaagcaaat gaaagtagaa ctcctgttat
    13741 ttatacagga gcaaatgatg gaatgctgca tgcttttaat gctgatacag gcgttgagct
    13801 tttcagttat gtaccaagtt cagtttattc aaaattaagt tctttgacat caccaagcta
    13861 tacacatcgc tatatagtgg atggcaatgc gtatgccgga gatgcgtata taggagcgac
    13921 accctcgtgg agaactattt tattaggaac gactggtggg ggtggtaagg gaatttttgc
    13981 gcttgatatt actgatccaa ctaatttttc agcttctaat gttctctggg agtttacaga
    14041 tgccgatctc ggttatgtac atggtcaggc aaagattgct attttgaata atggtgattg
    14101 ggttgcgatt attagtaatg gctataatag tacgagtgac aaagcctttt tgtatatagt
    14161 taatctacaa actggggcac tgattaagaa aatcgcaact aacagtacaa ctagtaatgg
    14221 attatctgca cccgcgcttg tggataccaa tggcgacaaa atgatcgatt atgtttatgc
    14281 gggagatttg caaggtaatg tgtggaaatt tgatatttcg catacgaata catcgcaatg
    14341 ggatgtggca tatggtagtg gtggaaccaa atatccgtta tttacagcaa ggaactcgag
    14401 taatcaggta caaccaatta catctccact agaaattgga gtgcatccga atggtggttt
    14461 ttttatttat tttggaaccg gtcaatttat tgcatccggc gacaatacca atactcaagt
    14521 tcagactttg tatggtattt gggataatgg tacgagaata agtgaaacga atcgtagtac
    14581 attgcagcag cagacgattg aaactcaaac aacatcttcg ccattcagac gaactgtctc
    14641 tacaaatgcg gttaactggg cgagtaagcg agggtggtat atggatttat tgaaacctcc
    14701 cagttcaagt aatcaaggag aacgctctat tgtcatgcca ctccttaatt ttggaagaat
    14761 cttatttaca actctaattc cttcaagtga tccttgcgca tccggtggta gcaattgggt
    14821 gatgttattg aatggtgaaa cgggagggat gttaacagca cctcattttg atacagataa
    14881 aaacggtcta ctgaacagta gcgatcaaat tattgctggt gtaggatctg atggggttcg
    14941 aagtgagtcg gttgcgatta gtgcaggtaa cttgctccat ttgatcgcag ggacaacgac
    15001 cggttcggtt gaaacaatta cggttagtgg aaatccactg actccaagac gttcttggcg
    15061 gcaaattcag tagattattg catcacaaat tttttaataa gaacttgatc tgagtttaaa
    15121 ttgcttattt gggagaaata atgaattcca tttatcgtca ttgccctaac tcttttgcaa
    15181 agagacattt tttgtgttca ggttttacct tgatagaact aatgatcgca gtagcggtac
    15241 tcggtattat tgcagcggtt gcacttccat cctatcaaga tcatgtgcgc cgaacaaatc
    15301 gtgctgaagc taaaagtatt ttgctcgaga tgtcacaatt attggaacgg aactatacag
    15361 ctgtgaatcg gtatgatcag aaaagtgatg ggactggttt tactttatca acaacacaat
    15421 ctcccaaaac tgggactgca aaatatacga ttcaatttgc agcaggttca cttactcaga
    15481 atgcctatac tttacaagca gttccgactg gatcaatgac cggtgatgca tgttcaacat
    15541 tgacactgac gaatactggt gtaaaagggg taggagctgg cgctactcta gatgcggcta
    15601 cctgctggca gcgttaagaa tgattctttt gttataaaaa ttaccctaat ttgatcaata
    15661 atcgccccaa agagcctgga tacttgctaa tgctgctaaa ccagcgctct cagttcgcaa
    15721 tgttctttga ccgaggcgaa tcagtataaa ccctgtagta attgctagct tttcttctga
    15781 gagcgttaaa ccaccctcag gtcctaccag taaaataaaa aaatctttgt tgttgggttg
    15841 gagctctgac aattttattt gagctgttgg tgtgagcatt aatttggttg atgatgtaat
    15901 tgaacgatca gcttgattgt ttttaaattg gctaagccag tcagtatatg aaactaatgg
    15961 caaaagctca ggtattttat tttttccgca ttgctcacaa gccgaatgga tgatttgttg
    16021 ccagtgttcg gctcgttttt taactttttc tccatgaaga tgaacgacgc aacgatttgt
    16081 cataatcggt tggatgcgat taacaccaac ttcgactgct ttttgaatga tccaatccat
    16141 tttttcattt gtacaaatag cttgaactaa ttcagtcatc acaggagatt cacgattaat
    16201 atcgtggaac tgttgaattt gtactgtcac accattttta tcaatttgct caatttgacc
    16261 tgtgaactca cccccctgac cgttaaataa aataacttga ttgttctttt tgagccttaa
    16321 aacatgagct gcatgatgtg aaactccact tggcagcaag atagtttcgt taatagttag
    16381 tgggtgatta caatagaaac gtgggggcat agtgagtgga ttgatgtgcg tgaaaacggg
    16441 ttgatcatga tccatgataa tattatagat attaaaaaga acaatagttt ggagaagtcg
    16501 gtatggctag tttggaaaca ccagtttgtg agtttggatg gaaagcagtt gattttgatt
    16561 tactgggggt cgatggaaag cgatatagct tgtcttcatc caggggaagg agtgggctat
    16621 tggtgatgtt tatatgtaat cattgtcctt atgtgaaagc aatcagagat cgtattattc
    16681 gcgatataaa agagcttgag gcgtatggca ttaattccat cgcaatcatg tctaatgatc
    16741 cattggatta tccagaagac tcatttgaaa acatggccaa agtggctaag gaatataatt
    16801 atccttttcc ttatgtctgg gatgaatctc aagaaattgc aaaacgctat ggagctgttt
    16861 gtacaccaga cttttttgga tttaatgata aattagagct tcaatatcga ggacgttttg
    16921 atgcatcacg aaaggaagct gctcctttag acgcgcatcg tgatttattt gaagcgatgg
    16981 tacaagtggc aaaaactggt aaagggccga tggatcaaat accgagcatt ggttgctcga
    17041 taaagtggcg tactgatgga aatgcttgag caagttatta ccatagtcaa gcgcgtagca
    17101 gaaaaggatg ttatgccacg ctttatgcag gtaagtcgtc aacataaagg agatggaagt
    17161 ttctatacag aagcggatct tgctgcgcaa gcgagcttag tgacagaatt aacaaagcta
    17221 tatcctgtag ttgtgatggg cgaagaaatg tcgcaacaac agcaactcca atgctgggaa
    17281 gacggagctg caggattgtg gagcattgat ccgattgatg gcacgtctaa tttttataac
    17341 ggaattcctt attttgcaat ttctgttgct ttgatgcaaa acgggagagg cttacttggg
    17401 gttatttata atccagccac aaaagaacta ttctatgctg agcgagggaa aggtgctttc
    17461 ctcaatggag ttgccttgcc gataagaaaa tcgactgctt cattacagag cgctgtggca
    17521 aatgttgatt taaaacggtt atctcgaaaa ttagctgcaa aagtagcagc taaccctcct
    17581 tatgcttcgc agcggaatta cggtgcttgc gccctggaat ggtgttatac cgctgctggt
    17641 tattttgatc tctatttgca tggtggacaa aagccttggg attatgcggc aggaagcctt
    17701 attctggagg aagcaggagg tcatatgtgc ggttttgagc atgatgatta ttggttacag
    17761 acaccgtggc agcgctcagt tattgccgct ttagatttgc aattgtttga acagtggcgt
    17821 gattgggtta gggatcaata tttactgctc aaataagtga gtatgtttac cattcaagaa
    17881 gctattcttt ttttgttaaa agattgagta atacaatttg aagattctta ttttaggtgc
    17941 ggggcgagtt ggttctacag tagcagaaag cttggttagt gaatcgaatg atattactgt
    18001 agttgatatc gtttcgggac agcttgcttt gttgcaagat cgtttggatc tcagggttgt
    18061 tgttggaaat gcctcctatc cttcagtatt aaagcgtgcc ggcgctgaag atgctgatat
    18121 gattcttgcg gtaacggaga gtgatgaaat aaatctggta tcttgcaaac ttgcagcaag
    18181 cttattcaat acacccacaa aaattgcccg aatccgctca gccgaatatc ttgagcattc
    18241 tgaaattttt gcaaaagata atttctgtgt agattttgct atttgccctg aacagatagt
    18301 tacagaatat attgagaatc taatcgaatt tccagaagcg ctgcaggtat tggatttcgc
    18361 tcatggaaaa ataagtttag tagcggtaaa ggcgctgcaa ggggggccat tagtgggtca
    18421 gcagttgcaa gagttgcgcc gtcatgtacc caatgtggat acacgtgttg ctgctatatt
    18481 caggaaagat cggcccatac ttccagaagg tgatacgatc attgaagtcg gtgatgaggt
    18541 cttttttatt gctgcgactg aagatattag ggctgtaatg gcagaactac gtagtatgga
    18601 taagccagca aaacgaatca tgatcgctgg cggcggtaaa atcggtaaac gtcttgcata
    18661 tgtacttgaa aaaaactatc aaattagagt gattgagcga gatgtcaaga attgcgagta
    18721 tttggcgcaa gaacttcagg atacattagt gttgcagggt gatgtaactg atgaggaatt
    18781 gctggaaagt gaaaatgtag ctgaaatgga tgtgttctgc gcactgacta atgatgatga
    18841 aaacaatatt atggcttcat tgctcgccaa aaaaatgggg gcacgtaaag ttattgcttt
    18901 gattaatcgc agtatctatg ttgatttagt tcaaagcagt ggcatcgata ttgcgatttc
    18961 tccagcgcaa gtgacgatag gttcattgct cgcttatgtc agacaaggtg atgttgcagt
    19021 agtgcatagc ttacggcgtg gcgcggcgga agcgctagag ttgattgcgc atggtgatgc
    19081 taattcctcc catgtggttg gaaaaaaaat agaggaagtt cagttaccca agggcgctac
    19141 tattggtgcc attgtaagag gactacctcc cggagaagaa cgatgggaga ataaagctgg
    19201 tgataaagaa aacgattcgg gaagcagaaa taaagccaaa gtgattatag cgcaccatga
    19261 taccgtgatt gaatcggagg atcatgtcat tgtatttgct attaataaaa aaatgattcg
    19321 acaaatcgag aaattgtttc aggtgagcgc atcatttttg tgataactaa tatttaatga
    19381 gtcatttttt atcggttgcg aatttactcg gtaagatcat tgctgtgttc gctttgacca
    19441 tgttaatccc ctatgggatt gcaatttggt cagatgatgg ttcatctact atttttggtg
    19501 attcaattct ggttacattc atatcaggca ttgttataag ccttatcact cttccttata
    19561 agaaagaatt gcagattcgg gacggttttt tgctggttgt gttagtgtgg ttgttactcc
    19621 catgttttgg tatgcttcct ttacttaact atttccctga attgagtata gcaaaggcct
    19681 attatgaggc aacttcagga ctaaccgcga caggtggaac ggttttgacc ggattggata
    19741 cattgcctta ttcgattaat ctttggagag gggagatggt atggctgggt ggaatgggcc
    19801 tcattgtgtt agctgtcgca atcttgccta tgcttggggt cgggggcagg cagttattta
    19861 aagcagaaat acctggtcct atgaaagaag ctaaattgac ccccaggatt gcagaaacag
    19921 caaaggggtt atggcttgtc tacttggttc tgacaattgc ttgtttgatt gcttacaagc
    19981 tagcaggaat gaattggttc gatgcaataa tgcacgcatt taccacgctg ggcctcggtg
    20041 gtttctcttc tcacgatgct agctatggat attggaattc acccctaatt gagtttgtgg
    20101 cgatggtatt catgctcatt agtggaatta actatgcaac tcacttttta gtttggcgaa
    20161 caaatagcct ttcttcttac cagaatgacc cagaaataag accttatatc tatatcgtgt
    20221 tatcgagctg catttttctt gcagtttatt tgtggttaat gggcgtttat gatgatcctt
    20281 tgcatgcgct tcgctatgcg gcttttaata ctatttcagt ggctactacg actggctata
    20341 gcaacacgga ctatagttta tggcctattt ttgcaccctt atggatgctg tttttgtcag
    20401 gcttttgtac ttcgtcaggg tcaacgggtg gtggtattaa aatgatacgg gtacgtattt
    20461 tgtaccgaca atttttccga gaaatgatta cgattatgca tcctcaagct atttctcctg
    20521 ttaagcttgg tcgaaacatt atctcaaatc agattatttt tgcagtgctt gtttttttat
    20581 ttgtttattt atcttcgatc attttaataa cgttgctgct tacactgagt ggacttgatg
    20641 agatgactgc tttctcagca gcggtcgcga gtttcaataa cctagggcca ggattaaatc
    20701 agatcggtcc ggcaactact tacgcatcgc tttcagattt ccaaatctgg atatgtagct
    20761 tctctatgtt attgggacga ctggagtttt atacattact gattgtattt acagcagctt
    20821 tctggcggaa gtgattctgt atcgttatgt ttcgccctat aatagagcta ttgtgttaat
    20881 gaatcgtttg ctagttcaag gggaagtgat ggagtgcttt ccatatatag acgagaataa
    20941 aagggagcat agaaatgacg gaagcaacaa gtggaaatga tttagtaggg aaaaagatca
    21001 tgatcgttga tgacagcaat acgatccgtc gcagtgctga aattttcctg gctcagtcgg
    21061 gttgtgaagt tatcctagcg accgatggtt ttgacgcgat ggctaaaatc attgatcatc
    21121 aacctgatgt tatttttctt gatatcgtaa tgcctcgcct ggatggttat caagcttgca
    21181 tgcttatcaa aaagaacccc cgctttcatt ctgttccggt aattatgttg tcaagtaaaa
    21241 gtggcttatt tgatcgtgct cgtggaagaa tggtcggatc agatgaatat ttaactaagc
    21301 cttttaccaa agaaggttta ctcaacacga taaaaaaaca cacgcagcaa caaaataaag
    21361 ttgcttgata aaataaccta ttttgtatgg ttatagtggt tagaacatgg ataatgaaaa
    21421 gaaatatgat atcgatatag tggttagaac tgcttatcta tcggagcaat cagatgagga
    21481 ggcgggtcgc catgtgtttg cttacacaat tactatttcc aatacgggtt caattgcttc
    21541 gcagctgata agccgacatt ggattattac gagtggtgat ggaaatgtac atgaagtgcg
    21601 ggggttgggt gtcgtcggtg aacaaccctt gctaaagcct ggtgatagtt ttgagtatac
    21661 aagtggcaca gcgatatctg cattcgctgg ctctatgaaa ggaagctatc aaatggtcgc
    21721 tgaggatggt gttcattttt cagtagaaat acctgaattt attttaagtg tcccgagagt
    21781 tctacattga tttttgtctt aagctataaa gttaattgat gctactcgct accgagcatt
    21841 attaagtagc cgcgttatat tcttaccctt gtatgcaata tggtaggcta tttctcattc
    21901 cttctccctt aggtgaagga gccattgatt ggatcattcc aagccatgtt aagcagtgta
    21961 ttgcgaattt aacgtacttt gttgtagagc atccgaaaac cgcgagacat tttctaaagc
    22021 aaattgcttt gcaacgaccg attcaagcta tcacgctaca aaccctggat aaacatacac
    22081 ctattaatga attagacagc ttgcttgggc ctatttatgc aggaaatgat atgggtttgc
    22141 tatcagaggc aggatgcccg gctattgctg atcctggtgc aaatctagtc cgaatagctc
    22201 atgaaaagca aatacaagtt gttcctctag tggggccatc ttcgatattg ttggcactaa
    22261 tggcttctgg gctaaacgga cagtgttttt ccttccacgg ataccttcca atcgataagg
    22321 tatcgtgtgc caagaagata attcagatgg agtcggaatc attcaaattt aatcaaactc
    22381 agatttttat cgaaacacct tatcgaaata agaagttatt cgaattgctg gctatgcatt
    22441 gccaaggaag cacgcactta tgtatagcga gcaatcttac gttgcaagat gaagtaatta
    22501 acacaaaaac aataaaagac tggcaaacta atttgccaga ttttgaaaaa aaaccaacag
    22561 tttttctact acatcggtat caaacctaag agcaaggtgg agagtttttt gagtaatttc
    22621 ttgctacttt gtcatacaaa gtccttttgc ttattcattt ttcttttgaa tgaattcaat
    22681 cttataacca tcaggatcct caacaaatgc gatgactgtc gttccatgtt tcatggggcc
    22741 tgcttcacga atgactctac cacctaattt tctaacgcta tcgcaggctt gatatgcatc
    22801 cgctacttct atcgcaatgt gtccatagcc ggtcccgata tcatattttt ctgtatccca
    22861 attgtgagtg agctcgagaa ctgcaccttc tgattcatgc tgataaccaa cgaaagctag
    22921 ggtaaatttt ccttccggat aatctttctg acggagcaat ttcatcccga ggacttgagt
    22981 ataaaactga agcgagttgt cgagatttcc aactcgaagc atcgtatgaa gtatgcgcat
    23041 taaagtttaa ccatttcaaa atcatcttta cgagcaccgc attctggaca tgtccagttg
    23101 atgggcacat cttcccaacg tgtacccggg ggaataccct cttgtgggga accttctgct
    23161 tcatcgtaaa tatatccgca tatcaggcac atgtaacggt gataaactgg attttgatcg
    23221 gttgacataa tagattgata tatcctttta agttaaaata ctattttacg ggattattgc
    23281 atgttacaac caccacctat cgtactatcc tttgctgcaa gtgatccgag tggtggtgca
    23341 ggcattcaag ctgatattct tacattagcg agtatgggtt gtcatcccct ttctgtagtt
    23401 actgcaatta cggtccaaga tacagttggt gtggaaagtt tcctgccgct tgagtcggaa
    23461 tgggtcgcag atcaagcgcg aatcgtgctt gaggatatgc cggttcatgc atttaaaatc
    23521 ggtgtgctag ggagtgtcga agcaatcgtg accattgctg aagtggttgc ggattatcct
    23581 aatatacccc ttgtgcttga tccggtgctt gcatcgggtc gtggcgattc atttgctaat
    23641 gacgaaattc ttcaagcgat gcgcgattta ctttttccac aaacgacaat cattacacct
    23701 aatagtttgg aagcaagaag gttaatgcag gagaatgaag aagatcctga aatagtcgac
    23761 ctgagtcaaa gtgcaaaatc ttttttgcgt ttaggctgtc aatatgtctt gattacaggt
    23821 acacatgaaa acacttctaa agtaattaat acgctttatg ataagaatgg tgttgttcga
    23881 gttgatgagt ggcaaaggtt gcctggatca ttccacggat caggttgtac tttgtcttca
    23941 gcgatatcgg ctgcattggc gcatggaatg tcagtatcag aaggtgtata tgaagcgcag
    24001 gattatactt ggaacacgct aaaatcagga tttcgcccag ggatgggaca atatttgcct
    24061 gatcggtttt tttgggcttc caatgaaggt gaatcagacc agaagtaaga caccgatggt
    24121 gcagcgatct agaatcgaag gattgtatgc gattactccg gatattgaga atacaagcga
    24181 tttggttgaa aaagtagatc aagtactatc atccggtatt cagtacatcc aatatcgaaa
    24241 taaatctatt gctcatgagc aacgagtgaa tcaagcctgt gcgattaaga agcggtgtca
    24301 gcgatatggc gctaaattaa ttatcaatga tcatttggat atcgcgctcg ccgtcaatgc
    24361 tgatgggttg catgttggga aagttgatgt ttctatccag acagcaagaa cttatctggg
    24421 taagcataaa atgatagggg tttcttgcta caatcagatc gatttagcgc tagctgctga
    24481 gaaagaaggc gccgattatg ttgctttcgg tgcgttttat gcttcaaaaa ccaaaatcga
    24541 taccgttgaa gcgaaccccg atttactgat tcaagcgaag aaagttttac aaatccctat
    24601 tgtggcaatt gggggtatta atgtaacgaa tgcggctttg cttattttcc gtggctgtga
    24661 tgccattgct gtaagtgatg gtttatttaa tactgctaat attccctcta tcgcaaaaaa
    24721 atttaaacgt ttattttgag caaattcata tttttttaat taattgagaa accaaatgaa
    24781 tataagtaat cagcagttat atgatctttc ccagcaatat atacctggag gcgttaattc
    24841 accggtaaga gcgtttaaat cagtaggggg gacgcccatt ttttttcaac ggggggaggg
    24901 tgcttatttc tgggatgttg aaggtaaatg ttatattgat tatgtcggtt cctggggacc
    24961 tttaatattg ggacatgcac atcctcaggt tgttaaagca gttcaagaaa ccgcaattaa
    25021 tgggctatct tttggtgctc cgacaaaagc agaactggat ctggccgaac tactttgtaa
    25081 gttactacca tcgttagaac aagtgcgtct ggttagctca ggaacagaag ctgggatgag
    25141 cgttattcgg ttagcgcgtg ggtataccgg gagaagtcat atcataaaat ttgaaggctg
    25201 ctatcatggt cacgatgatg cattgttagt taaagcaggt tctggtgcat tgacttttgg
    25261 ccacccaagc tcggcaggtg ttcctgctga gacggcagca catacgcttg tgcttaattt
    25321 taatgatgta gaagggttaa agcgtacttt cactgagttg ggtgagcaaa ttgcagcagt
    25381 tatcgttgag ccggtagccg gtaacatgaa cttagttaag gcaacgccag ctttcttatc
    25441 agagctgaga agcttatgtt ctcgttatgg tagtgttctg attcttgatg aagtaatgac
    25501 aggctttcgt gttggtttaa cctgtgcaca agggatgtac caaattcaac ccgatttgac
    25561 agttttaggt aaagtgatcg ggggggggat gcccctcgca gcctttggcg ggaaacgaga
    25621 gatcatgcaa tgcctagcgc ctgtcggtgg cgtttatcaa gcaggcaccc tttcaggaaa
    25681 tcctgtttct gttgcagcgg gcttagcaac actacgagaa attcaggcag aaggttttta
    25741 ttcaacactt gccgtcagaa caacccagtt aacagatggc ttaacagcta tggcaaggaa
    25801 acataacgtg gcattttgtg cgcaagctgt gggcggcatg tttggtttat attttagcag
    25861 taagcttcct caaagttttt cagaggtaat ggcttgtgat aaagaagcat ttaatcgttt
    25921 ctttcatgcg atgctaaagg aaggtattta ctttgcgcct tcggcatttg aagctggatt
    25981 tgtttcttca gcgcattctg aggatgatct atctaaaacg ttagctgccg cagatcgagt
    26041 atttagttcc tggtaggaaa ttaatcaatt attgagaaat aaataaaaaa agcctgcttc
    26101 aaaattttga ggcaggcttt tttgtatgaa gcttgagtaa gcagtgctga ttagttattt
    26161 caaagttgaa atatattcag cgacagcttt tttctcttgc gcgctcagac gtaatacgat
    26221 catttgcatc gtattattga tatcgtttgc tctcgtacct tgccggaaag tttctagctg
    26281 agataaggta tagtccgcat gctgtccagc caacttgggg aagagtggtg gtatacctgc
    26341 tccattcggt gagtgacagc ttgagcacgc tggcactgaa ttttctagat tgccaccgtt
    26401 atatactcgt ttccccaatt ctaattgaga ttcatcgacg ttattgttag caggctgtgt
    26461 tttttgctgg gcatagaaaa cggctaaatc ctgcatatca tctacgctga gcggcgcgac
    26521 catcgatgac atgattggat tatctcgttt ggctggtgta tcctcttcca ttttaaaatc
    26581 ttttaattgt ttcagaagat attcagcgtg ctgtcctgca agcgttggat ttgttgggat
    26641 aacactattg ccatccgcat tgtggcaacc cgcacaaaca cctgaagcga tttctttacc
    26701 cttttcggca tcaccttgta cagttttggt agcatctgcc agaatttgat tagaaaatat
    26761 caacgcggta atgattacaa tatttttaat aactttcatt tttgagaaat tgttgttttt
    26821 taaggtttca ataaaaaaac actacgatta acgacgaatt ctaccaactt ttgctaacaa
    26881 cagtaaacgt taataggtaa taattcaatt atatatgaaa atattttttt acttattatt
    26941 gtttatcaac atcatcacgt tggtaattat tgcgatagtc gaattgaatt cgaataaaaa
    27001 taatagagtt atggagtttc atgctgagaa aattaggatt ttacctgcac aagttgcgtg
    27061 tttggagtgg ctaagtttat acggtgtggc acttcaacaa gctagaactg agcttcccaa
    27121 acttaatcta gatcattttg ttatcgaatc gcctatggac gaaattacag tttacatcat
    27181 tcatatagca gcactaaaaa ccaaacggga agtggataga caactcgccc aattgcaaaa
    27241 actaaatatt gcttatcaac tcattcaaga aaatgttggc taccttcaga aagactcgat
    27301 tatgcttgga atgtatcagg agcgatcggc tgcaatgaga aagcttgagg agttaagtag
    27361 tatgaatgtg aataacgcca ttatttcgga gcgtgatata gagcaagtaa aatacatatt
    27421 tttagaacca acggaagatg tgagaattta ccttcagcag cttgttgaaa aatttcctga
    27481 tagctatctt aaattaactc aatgcgatcg attctagaaa tacaacagaa atgaaaaaaa
    27541 caaaaaattg cagtcaacta tggagaaagt tgtggccatt caaataaaaa cgccaaagca
    27601 agtagaaata atgcggattg cgggtaggtt ggctgctgaa gtactggacc atattacacc
    27661 acatgtattg ccaggagtta cgacaggtga actagatgag atttgccatc attatatggt
    27721 taatgtgcaa aaaactatcc cagctcccct caattacgca ccgcctgggt atagcccttt
    27781 tccaaaatcg atttgtacgt cagtcaatca tcaaatctgt catggtattc cgggcgccaa
    27841 gaaacttaag aatggggata tcgttaatgt ggatgtgaca gtaatttatg acggttatca
    27901 tggcgatacg agtcgtatgt attatgttgg ggagccttcc atacaggc
//
LOCUS       DAOQKQ010000008        27547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_699_length_27547_cov_6.177906, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000008 DAOQKQ010000000
VERSION     DAOQKQ010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 27547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27547
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_699_length_27547_cov_6.177906"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="PKZ49_01020"
     CDS             complement(<1..372)
                     /locus_tag="PKZ49_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HND35171.1"
                     /translation="MQEKYHPQEIEQDAQQYWEKTAAFNATELSGKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFHRMRGYNVLQPMGWDAFGLPAENAAMQHGLPPAKWTY
                     DNIAYMRKQLKSLGFSIDWTRE"
     gene            495..1292
                     /locus_tag="PKZ49_01025"
     CDS             495..1292
                     /locus_tag="PKZ49_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HND35172.1"
                     /translation="MRLISNLSRWVVWLCILLLSACATSPTGRTQLAFMPDEELDSMG
                     LQAFENLKKEKPIEQGGKNVQFVKCIAQAITREVGGEWEVVVFEDASLNAFALPGRKI
                     GVHTGLIDLVDNQDQLAAVIGHEIGHVLARHSNERMSQQVSTKLGLSIVQAVAAPTTP
                     LGQAAVSALGVGAQYGVIMPFSRLHESEADMIGIDLMAKAGFNPLESINLWKKMSHAS
                     QGNQPPEFLSTHPSHDTRIEDLQARLATAQPLYQQAMAAGKKPKCVK"
     gene            1621..2490
                     /locus_tag="PKZ49_01030"
     CDS             1621..2490
                     /locus_tag="PKZ49_01030"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HND35173.1"
                     /translation="MSQKHPVIAVTGSSGAGTSTVKNSFEHIFRREKLNVAIVEGDSF
                     HRYDRESMKAAVAESAKNNGRPISHFGPEANEFEKLEALFKEYGETGSGMTRLYLHND
                     EEAAPYNQKPGTFTPWKAIASGTDLLFYEGLHGGAKSDTADVAKYVDLLVGVVPIVNL
                     EWIQKIHRDMNARGYSAEAVTQTILHRMHDYVHYITPQFSRTHINFQRVPMVDTSNPF
                     IAREIPTLDESMVVIRFRDYKDVDFPFLLNMLHDSFMSRPNTLVVPGGKMGMAIELIL
                     TPLIIDLAKKSKH"
     gene            2647..4845
                     /locus_tag="PKZ49_01035"
     CDS             2647..4845
                     /locus_tag="PKZ49_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HND35174.1"
                     /translation="MTSLLTGLNPEQAEAVTLPYQSALILAGAGSGKTRVLTTRIAYL
                     IQSGQASPNSLLAVTFTNKAAREMVTRLGALLPINTRGMWIGTFHGLCNRMLRAHYQE
                     ARLPQAFQIMDMGDQQSFIKRLMKALAIDEKAFPPRQIQWFINNAKEEGLRASQLEAH
                     DAFSRKMGEFYQIYEERCMQEGIVDFAELLLRCYELLLHHEALRHHYCDRFRYILIDE
                     FQDTNRLQYKWLKSLAGIGFPQHAAVFAVGDDDQSIYAFRGAHAGNMKEFERDFAVQK
                     IIKLEQNYRSHGNILDAANALIDNNQGRLGKNLWTAEGSGELIRVYNAATDFEEAAFI
                     VEEVKVLKAEGVPLSEIALLYRSNAQSRVLEHSLFNASLSYRVYGGMRFFERQEIKHA
                     LAYLRLVTQPEDDTSLLRIINFPARGIGSRSLEQLQDDAKQLGMSLWATALQKCGTAL
                     EQAKVQKGVTGFVALIKSMQQGCRDLPLPEIIRFVIAQSGLLAHYAADREGAERLDNL
                     NELINAAVSFVHESEDDSLISFLSHASLEGGEHQADQHQEALQLMTVHAAKGLEFHSV
                     FLSGLEEGLFPHENSRSEANGLEEERRLMYVATTRARRRLYLSFSQSRMLHGQTHYNV
                     PSRFIEEIPEHLLKWLQPKHQNLSNTDFNRGWRTTQNQLGGVSNLNHSVSSMSKQTTW
                     QIGRSVSHGTFGAGVIVNCEGQGKDLRVQVRFSRAGTKWLLLEYANLTLL"
     gene            complement(4848..5669)
                     /locus_tag="PKZ49_01040"
     CDS             complement(4848..5669)
                     /locus_tag="PKZ49_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HND35175.1"
                     /translation="MEARHVRGRQGLQWILSGFYYFKLSPFVWMLLSSTFLMVELTLQ
                     ILPVLGIFAFLLISPVLVAGVMVGCQSLDQGQRLQLEHLFVGFRKNTAPLITVGGFNL
                     IALVLIVGIFMLMGGDALIDMLVYGKRFGENELMGIMDNVLSAWLAALGLSIPLMMAI
                     WFAPLLIIFENLPPAIAIRKSFFACLNNMAPFFVYGITLLILFFLISTVTVKLLSLFG
                     AVPPPLILIILYIVILPTIFASIYASYQDIFPSEPPSEETNQNDEDTSSNSEANH"
     gene            complement(5728..6678)
                     /locus_tag="PKZ49_01045"
     CDS             complement(5728..6678)
                     /locus_tag="PKZ49_01045"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HND35176.1"
                     /translation="MSVFTSVTQEQLSDWLQNYSLGTLIELQGITSGIENTNYRVITS
                     TGKYILTLFEKLTTTELPYYLNLMAHLSNQGIPCPNPVPTQTRRLLGELNGKPASIVT
                     FLPGVSLTEPTIAHCAEIGTMLAKMHLAGLTYPAYMENPRGPKWWNIAAKAVMPFLSS
                     EDQKLLSTELEFQSAYRKINLPHGVIHADLFRDNVLFTETTVGGIIDFYFACNDALIY
                     DLAITLNDWCITEKIIFDDEKARALIRAYHCVRNLNSEEHMMWPIMLRAGALRFWLSR
                     LYDFHLPRPGELTHKKNPDHFRQILIDHQSRHTKLLEIWV"
     gene            complement(6753..7133)
                     /locus_tag="PKZ49_01050"
     CDS             complement(6753..7133)
                     /locus_tag="PKZ49_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HND35177.1"
                     /translation="MYQIMTILLFSTIWFGTVSAQSNTANKSEPASPPPPPDLINQSE
                     QEMPELPDLGADPSMEPQVTIKKGDEETIEEYRVNGQLYMIKVIPRIGKPYFLLNKRS
                     PVGEIHRGDMESGVSVPMWQLYRF"
     gene            complement(7191..7910)
                     /locus_tag="PKZ49_01055"
     CDS             complement(7191..7910)
                     /locus_tag="PKZ49_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HND35178.1"
                     /translation="MSLQEKLGGVMSLEKLWSTRESWRVFGIMSEFVEGTERLEHIQP
                     AVSIFGSARTDPSHPYYKLTEAIARQLSDAGFSVISGGGPGIMEAANKGAYFGKSPSI
                     GLNIQLPHEQHRNIYQDISQTFRHFFARKYMFVKFATAYVVMPGGFGTLDELMEALTL
                     VQTGKTRKMPIILVCSEFWNGLIDWFQKTLVQEGYIFPEDMHLIQIIDDPKQIVDAIF
                     QYYETSGFEPSPAEKELQLNL"
     gene            7928..10672
                     /gene="polA"
                     /locus_tag="PKZ49_01060"
     CDS             7928..10672
                     /gene="polA"
                     /locus_tag="PKZ49_01060"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HND35179.1"
                     /translation="MKTLLLVDGSSYLYRAFHALPDLRNRHNKPTGAIYGVLNMLRRL
                     HKDFQSDYSACVFDAKGKTFRDEIYPEYKAHRPPMPSDLVDQITPLHDCIEAMGWPLL
                     IVEGVEADDVIGTLAKQACSHGMQCIISTGDKDIAQLVNHAIKLVNTMNNETLDAEGV
                     VKKFGVNPAQIIDYLALVGDSSDNIPGVEKVGPKTAVKWLSHYQSLDNLILHANEITG
                     VVGENLRKALPWLSVARQLLTIKCDVSLPVDFEGLITKQQDIPKLIALYDELEFKTWL
                     RELKTSNTLASGDREQTENQVTQHQNNSNSRANTKQSDIHYETITTETQLDRWIIQCE
                     QAPIVSVDTETTSLDPMQARIVGISFSIESSKAAYIPLGHTYTGVFDQLNLDYVLNKL
                     KPWLEDATKIKVGQNLKYDKHVLANHGVMLNGIAHDTLLQSYVLESHQPHNMDNLALR
                     HLDIETIHYDAVTGKGASKIGFEQVAIEQATRYAAEDADITLRLHRYLYPKIEQDRSL
                     QFVYHQIEMPLLAVLFQIERNGVLLDSNLLHAQSRDLAQQLYHLENQACALAGQVFNL
                     NSPKQIQEILFEQLKLPVIKKTPKGAPSTDEDVLQQLALDYPLPKVLLDYRGLAKLKS
                     TYTDKLPQMVNSNTGRVHTNYAQAVAVTGRLASNDPNLQNIPIRSPEGRKIREAFIAP
                     EGSQIISADYSQIELRIMAHISQDAGLLKAFAADEDIHQATAGEIFGVPIEQVNAEQR
                     RYAKVINFGLIYGMSEFGLASQLGIERSAARVYIERYFARYPGVAQYMQQTRERAREK
                     GFVETVFGRRLWLPEINSTHHNRRQGAERAAINAPMQGTAADIIKLAMIAVHNWLINH
                     QFKTKLIMQVHDELVLEVPDAEVAIVQANLSQLMENIGTFDVPLRVKVGIGKNWEQAH
                     "
     gene            10691..12016
                     /gene="pepP"
                     /locus_tag="PKZ49_01065"
     CDS             10691..12016
                     /gene="pepP"
                     /locus_tag="PKZ49_01065"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HND35180.1"
                     /translation="MSNKEQMQVYRERRNRLLDQLTHGIAVIATAPMRPRNRDSYYPY
                     RFDSYFYYLTGFEEPEAVLVVVAENQQGNAEQILFCRDKNLEREIWDGFRYGPDAACE
                     RFKFDSTYPISRLDEMMPKLLANQSTVYTMIGQDPAWDTQITGWVNRVREQTRSGITA
                     PDTLADVRKILDEMRLFKDEYELTLIRQAANIAVDAHRRAMKHANPGLFEYEIEAELI
                     HEFRRQGALAPAYTPIVAGGANACVLHYIENNMRLQSGDLLLIDAGCEYGGYASDITR
                     TFPINGRFTAVQRDLYQLVLSAQDAAIKAVQPGHTWDDPHQTALTVLVQGFIDLGLCQ
                     GSVEGIIESEDYKRFYMHRTGHWLGLDVHDAGEYKQAGAWRELKPGMLLTVEPGCYIR
                     PADNVPNHFWNNGIRIEDDVLVTTEGHEVLTAAAPKTIVEIEEWMYERR"
     gene            12003..12347
                     /locus_tag="PKZ49_01070"
     CDS             12003..12347
                     /locus_tag="PKZ49_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35181.1"
                     /translation="MKEDDPRQIIISKIIEHRENNTSQAEYSTSVSPVSRWLFYLALI
                     PVTIIVFFVGFFFFSILLAILLVAMAGIGARIWWLRRKFKQAANAQEEIKSAEIEDAE
                     IIETVIHKTNKK"
     gene            12403..12729
                     /locus_tag="PKZ49_01075"
     CDS             12403..12729
                     /locus_tag="PKZ49_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35182.1"
                     /translation="MNSDSTAVRKADASSANALQVNIRMQPGEHTGQPIFSNLTSVQA
                     SQGVVVIDFGFIEPQMIQALSRLAQSGEKAPDTISAKMSCRMVVSVEAASQLAQQLSQ
                     LFVKKT"
     gene            12755..13174
                     /locus_tag="PKZ49_01080"
     CDS             12755..13174
                     /locus_tag="PKZ49_01080"
                     /inference="COORDINATES: protein motif:HMM:NF023376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HND35183.1"
                     /translation="MNQSNPLPVKIKIQQDERSGKPSYANFTTVQHGQGKIVIDFGFF
                     DPKLLQVSVNVDNKPLGVIDAHLSRRIILSQEVATQFAQQLSQLFTKHGDVKEPQSES
                     VMTDTLPSEIQDHSPEQGENLSENPKKGFRFPWFKKK"
     gene            complement(13213..13491)
                     /gene="infA"
                     /locus_tag="PKZ49_01085"
     CDS             complement(13213..13491)
                     /gene="infA"
                     /locus_tag="PKZ49_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HND35184.1"
                     /translation="MAKEELIEMNGTVDKVLPDSRYLVTLENGHQLVAYTAGKMKKNH
                     IRILAGDKITIELSPYDISNGRITFRHIENRNPAPTKHHASFNKNTRK"
     gene            complement(13604..13807)
                     /locus_tag="PKZ49_01090"
     CDS             complement(13604..13807)
                     /locus_tag="PKZ49_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HND35185.1"
                     /translation="MAMGTVKWFNDEKGFGFITPENGSTDVFVHYSAIQGGGFRTLRE
                     NQRVSYDTSPGPRGPQAVNIRPA"
     gene            complement(14050..14946)
                     /locus_tag="PKZ49_01095"
     CDS             complement(14050..14946)
                     /locus_tag="PKZ49_01095"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HND35186.1"
                     /translation="MNNLRLTRLWPGLVGLFLLISPLTSTATIIRLQTTLGVIDIKLH
                     DETAPRTVANFFEYLKSNAYDRSFFHRTIPGFIIQGGGYVWNQTTNAVNTIAKLSPVD
                     NEFSVTRSNVRGTIAMAKLPDKPNSATSEWFINLADNSADLNKQNEGFTVFGQVLGNG
                     MQVADAIAALPRTNAGSVFSTLPIIGTIEDHKLQEKNLVIVSRITSNKTDTPENDSDR
                     LFSYLEAFYSQYLSPANPLGSGQTVSATFENYYYRYYPTTQSYVGIANGEVYYLGPAS
                     GNQIISIGTFSNWLEQAVAAGY"
     gene            complement(14973..15434)
                     /gene="ssb"
                     /locus_tag="PKZ49_01100"
     CDS             complement(14973..15434)
                     /gene="ssb"
                     /locus_tag="PKZ49_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HND35187.1"
                     /translation="MASVNKVILVGNLGRDPEIRYMPSGDAMANLNIATTDTWKDKEG
                     EKQEKTEWHRVVMFGKQAEIAGEYLKKGSQVYIEGRLQTRKWTDKSNIERYTTEIVAD
                     RMQMLGSRSGGASYEPTDKDGEYNSANFSDKPTGQSKKSDRFDDLEDDIPF"
     gene            complement(15437..16822)
                     /locus_tag="PKZ49_01105"
     CDS             complement(15437..16822)
                     /locus_tag="PKZ49_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HND35188.1"
                     /translation="MSSLPNSEKMSPLEKRASVGLASVYGLRMFGLFIILPVFALFAE
                     HLPGGDNFTLVGIALGAYGLTQAILQIPFGWLSDRIGRKPVIYLGLVLFAIGSFIAAT
                     AVDIYWVIAGRIIQGAGAISAAVMALAADLTREEHRTKAMAMIGMTIGATFAISLMIA
                     PLLNQWIGVPGIFIMTGLLAIIAIIVVHKIIPDPIVSRFHSDAEVSKSRFSTALRHPE
                     LLRLNYGIFALHAVLMALWLVVPLSLREAGLAADHHWQMYLPVLVLSVLLMVPAIIYG
                     EKKAKLKPVFITAIALLTIGQGLIAFTSQSLWGIGIALVIFFTAFNLLEASLPSLISK
                     IAPVEAKGTAIGIYSSTQFLGAFVGASLGGYLYQHVGLTAIIIFCTMLLLVWLYFAFT
                     MKSPAAVRSRMYHVAEMDNQRANGLSKELAALPGVHEALVLANEQVAYLKVDLKGFDE
                     EKVVQLIKGGA"
     gene            16889..19720
                     /gene="uvrA"
                     /locus_tag="PKZ49_01110"
     CDS             16889..19720
                     /gene="uvrA"
                     /locus_tag="PKZ49_01110"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HND35189.1"
                     /translation="MELIKIRGARTHNLKNINLDIPRNKLVVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLQLMEKPDVDLIEGLSPAIAIEQKATSHNPRSTVGTVT
                     EIHDYLRLLYARVGDPFCPEHGIKLSAQSVSQMVDHVLRLPADTKLMVLAPSVVGRKG
                     EQQDLFDELRAQGFTRIRLDGSIYEIDQLPKLTKNKKHTIEVVIDRLKVSSEAKQRLA
                     ESFEMALRHGDGRAIAVEMETGQEHLFSAKFSCPVCNYALSELEPRLFSFNNPVGACP
                     KCDGLGQITFFDPARVVTLPHLSLAAGAIKHWDRRNQFYFQMLTAIAKHYEFDIETPF
                     EQLADTIKQIILFGSGKEKIQFTFLNESGRASKELYPFEGVIPNLSRRYKETDSQTVR
                     EELAKFINSRICPDCEGTRLCREARHIRVSEQAIYQISAWPLKQASAFFDQLVLAGHK
                     QEIAERIIKEISNRLQFLNNVGLDYLSLDRSADTLSGGEAQRIRLASQIGSGLTGVMY
                     VLDEPSIGLHQRDNGRLLDTLQQLRNLGNSVIVVEHDQDAIFLADHVVDMGPGAGEHG
                     GCIIAQGAPSEIQHHPASLTGQYLSGKRSIAIPAVRTPLNPERILTLSGVTGNNLKQV
                     ELNLPVGLLVCITGVSGSGKSTLINDTLYRAVAQQLYGNNTEPAPYQTLEGIAFFDKV
                     INIDQTPIGRTPRSNPATYTGLFTPIRELFAGVPQARERGYTPGRFSFNVKGGRCESC
                     QGDGVIKVEMHFLPDIYVSCDVCHGKRYNRETLEIQYKGRNINEILQMTVENAHTFFE
                     SVPVVARKLQTLLNVGLGYITLGQSATTLSGGEAQRVKLSLELSKRDTGRTLYILDEP
                     TTGLHFQDIDLLLKVLHRLRDQGNTVVVIEHNLDVIKTADWIVDLGPEGGDGGGRIIV
                     EGTPESIVEHPESFTGLYLKPILAAQIKR"
     gene            19724..20995
                     /locus_tag="PKZ49_01115"
     CDS             19724..20995
                     /locus_tag="PKZ49_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HND35190.1"
                     /translation="MNPRELLLNSFKIALEAVDPLKLIPAHLPAPPKRNTLVVGAGKA
                     AAAMAQAVEIHWSPDANLEGIAITRYGHGLPTQRITVIEAGHPIPDQNGEMATRAIME
                     SARILGSDDLMLCLFSGGGSSLLSMPIEGVTFEELKDINRQLIRCGASIQEINTVRKH
                     LSNVLGGRLAAACQAPIHCLIISDVTGDNPTHVSSGPCSPDPTTYADALALIKHYAID
                     ISSNVSAILQQRSQNPADETPKAGDTAFRNVKSRVIATAHQSLVAVESFFREHKITPM
                     VLGDTVTGEAREVAKVYAALAREIRHYGTPFRPPVALISGGETTVTIKGQGRGGRNTE
                     FLLSLAIELNGLEQVYALACDTDGIDGTENNAGAILTPDTIARAQLLGLKPIDLLNNN
                     DSYQLFEWLNDLVVTGPTRTNVNDLRVILIT"
     gene            complement(21036..22844)
                     /locus_tag="PKZ49_01120"
     CDS             complement(21036..22844)
                     /locus_tag="PKZ49_01120"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HND35191.1"
                     /translation="MWSDPAIAYLDKARSSTNHDIARRMRFGGLFYLLCAISIVLFSP
                     DLREQSSIIAFIVCFFILALLRLFLFKQVELNTFSDALIERLIFTVYVLTALSWVGFF
                     IIILATINEISDGVLIAIAVTIGFLSGGISATAPRMALMTTFASLIFLPCLFSLAIFL
                     PIDSGWTVLFVGLAYFIYCVHSGKLQNHDYWIIRQQASLLETQTKDLEQARSQAEQAN
                     KAKSAFLAAMSHEIRTPMNGVLGMAELMANTPLNAVQSQQLAIIRSSGRTLLRVIDDI
                     LDFAKIEAQKLAIVNRAFSPSHLIQEIKLLFRPKADETGLDFIIDIDTPLPTLLTGDP
                     DRIKQVLFNLLGNAFKFTPAGSVQLSVKYIDIPESDIVELHFTIIDTGIGISHEDQKL
                     LFQDFRQVGESTQHIQGTGLGLAITQNLLTLMGGTIALTSESGRGSQFKVCIPLKHAT
                     EVNSQVESKTVEPINSVKLKSTDRRLKVLLVEDNVVNQMISRAMLEHLNCEIAIASDG
                     KAAIAEYQNQHPDLILMDCNMPIMDGFEATRHIRRLEQQTNQPRIPIIAVTAHAFDHI
                     RQDCLDAGMDEHLSKPFDLNQLAMLLNQFRNSPASK"
     gene            23113..24477
                     /locus_tag="PKZ49_01125"
     CDS             23113..24477
                     /locus_tag="PKZ49_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HND35192.1"
                     /translation="MSMQSINPAAGQITPSFSIWQADQINTALDQVESAQARWAALSY
                     EQRGQCFRRLAHVFRQNSESYAHLITEEMGKLLREARAEIEKCAMGCEHYADHAASYL
                     RDEVIASDASRSIVVYQPLGTILCIMPWNFPFWQLIRAAAPAMMTGNTVVLKHASNVP
                     QCALALEAAFQQARFPTHTFRTLMISAAQAEQVIADRRIAAVTLTGSSAAGRKVAAIA
                     GQHLKKCVLELGGSDPFIVLDDADIEWTAQQALMARLQNMGQSCIAAKRFILLETIAD
                     GFVERLQALISELRSGDPKDAATTIAPMARVDLRDELHAQVERSLALGAKLRAGGFSI
                     GEQGAYYAPTILDHVQPGMPAFDEELFGPVFAIARVSNAEEAIKLANQTQFGLGGSVW
                     TGDLARGEALARQIHAGCTFVNGIVKSDPRLPFGGIKDSGYGRELSYHGIREFVNIKT
                     IWIR"
     gene            complement(24573..24872)
                     /locus_tag="PKZ49_01130"
     CDS             complement(24573..24872)
                     /locus_tag="PKZ49_01130"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HND35193.1"
                     /translation="MSHTTLEKLNRVFARHDTIDSVLIYGSRAKGNYRRGSDIDLTIK
                     GSEISFAEFMQIEDQIDDLMLPYTVDLSQYRLLENTDLIAHIDRVGRVIYTRPRK"
     gene            complement(24893..25306)
                     /locus_tag="PKZ49_01135"
     CDS             complement(24893..25306)
                     /locus_tag="PKZ49_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HND35194.1"
                     /translation="MNDPDIRWQQRFANYQKALLQLQNAVELSNQRALSPLEKQGVIQ
                     AFEFTHELAWNLLKDFLQDQGNQNVRGSKDATREAFKVELIQDGEQWMAMIQSRNISS
                     HTYNEHTAEQLVNAIIHDYYPLFRALKKEIEKYQP"
     gene            complement(25471..26985)
                     /locus_tag="PKZ49_01140"
     CDS             complement(25471..26985)
                     /locus_tag="PKZ49_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HND35195.1"
                     /translation="MSLAILYSRALSGMDAPLVTVETHLSNGLPSFTIVGLPETEVKE
                     SKDRVRAALQNSHFKFPAQRITVNLAPADLPKESGRFDLPIALGILAATAQIPHDQLD
                     QFEWVGELSLSGELRPIKGALAMTYSASRSGRSFVLPKQNAVEAALVSDSIVYPAASL
                     LEICAHLCNETPLQRYSSTITPDTHSMNSPLTIDLQDVKGQVQAKRALEIAAAGNHSL
                     LMVGPPGTGKSMLAERLITILPTMSQQEALETAAIQSLAQGQFEIAHWKRRPFRSPHH
                     TASATAMVGGGSIPRPGEISLALNGVLFLDELPEFDRKVLEVLREPLESGHITISRAA
                     RQTNFPARFLLIAAMNPCPCGYLGHPSGKCHCTPDQVARYRGKISGPLLDRIDMQIEV
                     PSLPQNDLMQSQSIGEKSNIIRERVEKAHQLQYDRQGKTNSRLSVKEIDRLCVLDTAS
                     ENLLKQAINRLSLSARAYHRILKLARTIADLGNAERINHQHIAEAIQYRRLDKH"
     gene            complement(27070..27357)
                     /locus_tag="PKZ49_01145"
     CDS             complement(27070..27357)
                     /locus_tag="PKZ49_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HND35196.1"
                     /translation="MLNKNILDEIGTKINEVIAHGPAKDIEKNLRTILTSMISKLDLV
                     TREEFDVQQEVLKRTRTKLTELETKVAALEGQLKPSPSETGTVTSDSNYDG"
BASE COUNT         7820 a         5594 c         6438 g         7695 t
ORIGIN      
        1 ttcacgcgtc caatcgatac tgaaaccaag acttttaagt tgcttgcgca tataagcaat
       61 attgtcataa gtccattttg ccggtggtag accatgttgc attgcagcat tttctgctgg
      121 caaaccaaat gcatcccaac ccatcggttg taaaacgtta taaccgcgca tgcgatggaa
      181 acgtgatagc acatccccaa ttgtatagtt tcgaacatgc cccatatgaa gtttaccaga
      241 cgggtacgga aacatggata agcaataata tttaggttta cctgaaagct ctgtggcatt
      301 gaaagcagcc gttttttccc aatattgctg tgcatcctgc tcgatttctt gtgggtgata
      361 tttttcttgc atgcaacatt tatcctggtt taatcgaaaa tcgaattata acttgaactg
      421 cttcgcttga tgcgaaccaa cgcatcatgg tagcctcatt ttaaatgtga aattaattcg
      481 taaaggagct acaaatgaga ttgattagta atttaagccg atgggttgtt tggctgtgta
      541 ttctgttatt gagtgcctgc gcgacgtcac caactggaag aacgcaatta gcttttatgc
      601 ctgatgaaga attagatagc atgggtttgc aggcattcga gaatttaaaa aaagaaaaac
      661 caatcgagca gggtgggaaa aacgttcagt ttgtgaagtg tattgctcaa gcaattaccc
      721 gagaagtagg tggtgaatgg gaagtagtgg ttttcgaaga tgcttcgttg aatgcctttg
      781 cattgccggg aagaaaaatt ggcgttcata ccggattaat cgatctagta gacaatcaag
      841 atcaacttgc agcggtcatt gggcatgaaa ttgggcatgt gctcgcgcgg catagtaatg
      901 agcgcatgtc gcaacaggtc agtacaaagc ttggattatc gattgtacag gcagtagcgg
      961 caccaacgac accattgggg caagctgcgg taagtgcatt aggggttggt gcccagtatg
     1021 gggtaatcat gccatttagt cgcttgcacg aaagcgaggc ggatatgatc ggcatcgatc
     1081 tgatggcaaa agcgggattc aacccgctag aaagcatcaa tttgtggaaa aaaatgagtc
     1141 acgccagtca agggaatcag ccgccggagt ttttatcaac gcatccttcg catgataccc
     1201 gtattgagga tttgcaagcg cggcttgcaa cagcacagcc gctttatcag caagcgatgg
     1261 ccgcaggtaa aaaaccgaag tgtgtaaagt aatcagttgg attctaagac tataagaaag
     1321 ttgttcaaat cagtggcagt aataagaatg attagggcga tgatggtatg gtgaatgcag
     1381 cagagttgac caagattatt ttagaagctt attctacaaa gcgggtattt ttattcatga
     1441 ccttatattg gataaataat tgatatataa gaataattta tgattctttg attctgcctt
     1501 tcaatttttg attgcccaaa tctgctcgaa agcaggtatc atacgctgtt aaagttataa
     1561 caaaaaaaat cttactattc attatttttt attagtgtat ttatagggag aaagtagcaa
     1621 atgtcacaaa aacatcctgt tattgccgtg actggatctt cgggcgccgg tacatcgact
     1681 gtcaaaaata gttttgaaca tatttttcgt cgtgaaaaac tcaatgtagc gattgttgaa
     1741 ggggatagct tccatcgtta tgatcgagaa tcaatgaaag ctgccgttgc agagtcagca
     1801 aaaaacaatg ggcggccgat tagtcatttt ggcccagaag ccaatgaatt tgagaaattg
     1861 gaagccttgt tcaaagaata tggggaaaca ggttctggaa tgacgcgttt gtaccttcat
     1921 aatgatgaag aagcagcgcc atataatcaa aaaccaggaa cctttacccc ttggaaagcg
     1981 attgcatcgg gtaccgattt gcttttttat gaaggccttc atgggggagc aaaaagcgat
     2041 actgcagatg ttgcaaaata tgttgattta ttggttggtg tggtaccaat cgtaaatctg
     2101 gaatggatac aaaaaattca tcgtgatatg aatgcacgtg gttattcagc agaggctgta
     2161 acacaaacga ttcttcatcg tatgcatgat tatgtgcatt atataacccc gcaattttca
     2221 agaacccata ttaattttca acgcgttccc atggtggata cttcgaatcc attcattgct
     2281 agagagatac caacgctcga tgaaagtatg gtggtgattc gattccgtga ttacaaggat
     2341 gttgattttc cattcttatt aaatatgttg cacgattctt tcatgtctcg tcccaatacc
     2401 cttgttgttc cgggcggtaa aatgggcatg gccattgaat tgattttaac gccacttatt
     2461 atcgatttag caaaaaagag caagcattaa tgaagcgtta gttgtaacac gctgataatc
     2521 taactgaacc ttaccgaatt tcaacgagtt ggtaaggttc ttttgtttct gaagaagatt
     2581 taaccccgat taactaggaa tgattactca atcctagcaa accaaacgtg ctggacacat
     2641 tctctcatga cttctttact tactggattg aatcccgagc aggcagaagc tgttacatta
     2701 ccttatcaat ctgctttaat tctcgcgggg gcgggaagcg gtaaaacgcg ggtactgacc
     2761 acgcggattg cgtatttgat tcaatcaggt caggcaagtc ccaatagctt acttgcggta
     2821 acttttacca ataaagctgc acgagaaatg gtgacacggt taggggcttt attgccaatt
     2881 aatacgcgtg gtatgtggat tggtactttt cacggcttat gtaatcgtat gttgcgcgcc
     2941 cattaccagg aagcccggtt acctcaagca tttcagatca tggatatggg agatcaacag
     3001 tcgttcatca agcggctgat gaaagcgctt gccatcgatg aaaaagcatt tccaccgcgg
     3061 caaattcagt ggttcatcaa taatgcaaaa gaagagggtt tgcgcgcttc acagttagaa
     3121 gcgcatgatg ctttttcccg taagatggga gagttttatc aaatttacga agaacgatgt
     3181 atgcaagaag gcatcgttga ttttgctgaa ctcctgctgc gttgctatga actgctgctt
     3241 catcatgaag cattgcgcca ccattattgc gatcgattcc gttacattct gatcgacgaa
     3301 tttcaagata ctaatcgatt gcagtataaa tggctcaagt cattggctgg aattggcttt
     3361 cctcagcatg cagccgtttt tgcggtgggt gatgatgatc aaagtattta tgcatttcgc
     3421 ggtgcgcatg ctggaaatat gaaagaattt gagcgcgatt ttgctgttca gaaaatcatc
     3481 aaacttgagc agaactaccg gtctcatggc aatatcctcg atgcagccaa tgcgctgatt
     3541 gataataatc aagggcgttt aggtaagaat ttatggacgg cggaaggcag tggggagtta
     3601 attcgagtct ataacgcggc taccgatttt gaggaagcgg ctttcattgt agaagaggta
     3661 aaagtgttaa aagctgaagg tgtaccgctc tcagaaatcg ccttacttta tcgatcgaat
     3721 gcgcaatcaa gggtgttgga gcacagcttg tttaatgctt cgttatcgta tcgagtctat
     3781 ggtggtatgc gattttttga gcggcaagaa atcaaacatg ccctggctta cctccgatta
     3841 gtgactcaac cagaagatga tacctcattg ttacgcatca ttaattttcc tgcgcgtggg
     3901 attggatcac gtagtcttga acaattacag gatgatgcga aacagctcgg aatgagttta
     3961 tgggcgactg ctctgcaaaa atgtggaacg gcgctcgaac aggctaaagt tcaaaaggga
     4021 gtaacagggt ttgttgcgtt gattaaatcg atgcagcagg ggtgtcgtga cttaccttta
     4081 ccagaaatca ttcgttttgt gatcgcacaa agtggcttat tagcgcatta tgcggctgat
     4141 cgtgaaggtg ctgagcggct tgataatttg aacgagctga ttaacgctgc ggtaagtttt
     4201 gtacatgaat cggaagatga tagccttatt tcatttttgt cacatgcgtc actagagggt
     4261 ggtgagcatc aagcggatca acatcaagaa gcattacagc tcatgacagt tcatgcagcg
     4321 aaaggattag agtttcatag tgtgtttttg agtggtttgg aggaggggct ctttccacat
     4381 gaaaacagtc gatcagaggc aaatggtctc gaagaagaac gccgtttgat gtatgtggca
     4441 acaacgcgtg cacgccggcg gctgtatttg agtttttctc agagtcgtat gcttcatggt
     4501 cagacacatt acaatgtacc ttcacggttc attgaagaaa tcccggagca cttattaaaa
     4561 tggttacaac ctaagcatca gaatttatcg aacacagatt ttaatcgtgg gtggagaaca
     4621 actcaaaatc aattgggtgg tgtatctaat cttaatcatt cagttagtag tatgagtaaa
     4681 caaactacct ggcagattgg gcgctccgtt tctcatggaa cttttggtgc tggtgtcatc
     4741 gttaactgtg aaggtcaggg aaaagatcta cgagttcaag ttcgatttag tcgtgcagga
     4801 accaagtggc tcctgcttga atacgccaac ttgacgctac tctgatatta atgattggct
     4861 tctgagttgc tactcgtatc ttcgtcgttc tggttagttt cttctgaggg tggttcagag
     4921 ggaaaaatat cctgatagct tgcgtaaatg gaagcaaaga tcgttggtag tattacgata
     4981 tagagaataa tgagtatcag tggcggtggt acggcaccaa agaggctcaa tagttttact
     5041 gtgacggtag aaatcaggaa aaacaatatc aatagtgtga tgccatagac aaagaaaggc
     5101 gccatattgt ttagacaggc aaagaaactt tttcgtattg caatcgcagg gggtaggttc
     5161 tcaaaaataa tgagcaaggg tgcaaaccat attgccatca tcaaaggaat gcttagccct
     5221 aaagcagcga gccaggctga taatacgtta tccatgattc ccatcaattc attttcgcca
     5281 aatcgttttc cataaacgag catatcgatg agggcatcac cacccattag cataaaaata
     5341 ccgacaatga gaacgagcgc gattagatta aatccgccaa ctgtgatcag tggagcggta
     5401 tttttcctaa aaccaacgaa taagtgttca agctgaagtc gctggccttg atcaagactt
     5461 tggcagccaa ccattacgcc agcaactagg acgggtgaaa tcaataaaaa ggcaaagatt
     5521 cctaaaacag ggaggatttg caaggtgagt tcgaccatca gaaatgtgga gctgagtagc
     5581 atccaaacaa acggagataa cttaaaatag taaaaaccac ttaagatcca ctgtaaacct
     5641 tgtctcccac gaacatgccg agcttccatg aattcttttt ctccaattga tagatgaaag
     5701 gatagtgata ctgatactgc gtaaaagcta cacccaaatt tccaaaagtt tcgtatgacg
     5761 tgattgatga tctatcaata tttgtcggaa atgatcgggg tttttcttgt gcgttaattc
     5821 tccagggcga ggcaaatgaa aatcgtataa gcgcgataac cagaatctta atgcacccgc
     5881 tctgagcatg atgggccaca tcatgtgttc ttcagaattc aagttacgaa cgcagtgata
     5941 agcgcgaatt agcgcacgtg ctttttcgtc atcaaaaata attttctcag ttatacacca
     6001 atcattgagt gtaatcgcta aatcgtaaat taacgcatca ttacaagcaa aatagaaatc
     6061 aatgatgcca ccaacggtcg tttccgtaaa caatacgttg tcgcggaaca gatctgcatg
     6121 aatgactcca tggggaagat tgatctttcg ataagcagat tggaattcaa gctctgtact
     6181 tagcagtttc tgatcttctg aggataagaa aggcattacc gctttagctg ctatgttcca
     6241 ccatttgggc ccccgtgggt tttccatata ggcagggtag gttaatccag ctagatgcat
     6301 ttttgcaagc atggtaccga tttcagcgca gtgcgcaatg gttggttcag tcaacgatac
     6361 gccaggtaga aaagttacga tactggctgg ctttccattc aattcgccaa gtagtcgccg
     6421 tgtttgggtt ggaaccggat taggacaggg gataccttga tttgataaat gagccatcaa
     6481 attaaggtaa tacggtagtt cagtagttgt taatttctca aataatgtaa gaatgtactt
     6541 cccggtagaa gttattaccc gataattggt attttcgata ccggaagtaa tcccttgaag
     6601 ttcaataagc gttcctagcg aatagttctg aagccaatca gaaagttgtt cctgggtaac
     6661 cgatgtaaaa accgacatga aataaagtaa agagaaactg aaaaacgtta ttcaacccaa
     6721 ctgaatcatt acctataatt gttgggttaa atttaaaatc gataaagttg ccacattgga
     6781 acactaacac ccgattccat atcaccgcgg tgaatttctc caacagggct gcgtttattg
     6841 agcagaaaat agggcttacc gatacgagga atgactttaa tcatataaag ctggccgtta
     6901 acccgatatt cctcaatcgt ttcttcatcc ccctttttaa tggtgacttg cggttccatt
     6961 gagggatcag cgccaagatc aggcaattcg ggcatttctt gttcagactg gttaattaaa
     7021 tcaggggggg gtggagggga agcgggttca gatttatttg cagtattgga ctgagcagat
     7081 accgttccaa accagattgt actgaataat aaaatagtca taatttgata cataccgatc
     7141 ctgatttctg tagggcgcat agcagccatt ctatccgaaa aataaatgat ttataaattt
     7201 agctgtaatt ctttttcagc gggggaaggt tcaaaaccag aggtttcata gtattgaaag
     7261 atggcatcga cgatttgttt agggtcatca ataatttgta ttaaatgcat atcttcagga
     7321 aaaatgtaac cttcctggac cagcgttttt tgaaaccaat cgattagacc attccagaat
     7381 tccgaacaga ccaagataat cggcattttt cttgtttttc ctgtttgaac gagcgttaat
     7441 gcttccatta actcatcgag cgtaccaaac ccccctggca ttaccacata agctgtggca
     7501 aattttacga acatatattt gcgagcaaag aaatggcgaa aggtttggct aatatcttgg
     7561 tagatattgc gatgctgctc gtgtggtagt tgaatattga gcccaatact tggcgatttc
     7621 ccaaaataag cccccttgtt ggcagcttcc ataattccgg gaccacctcc tgagatgaca
     7681 gaaaagccgg catccgacaa ttgtcgtgca atcgcttcgg ttaatttata ataggggtgg
     7741 cttggatccg tgcgtgcact gccaaagata ctgactgccg gttgaatatg ttctaagcgt
     7801 tcggttcctt cgacgaattc ggacatgata ccgaacacac gccaggattc ccgagttgac
     7861 caaagtttct ccaatgacat gacgccacct aatttttctt gcaagctcat ataggtcctt
     7921 tctttagatg aaaacactct tgttagttga tggctcgtct tacttgtacc gtgcttttca
     7981 tgcattgcct gatttgcgta atcgtcataa taagccaacg ggtgccattt atggggtgct
     8041 gaatatgtta cgccgcttac ataaggattt tcagtcagat tatagcgctt gcgtgtttga
     8101 tgcaaaaggc aaaacatttc gcgacgagat ctatcctgaa tataaagcgc atcgtccccc
     8161 catgccgagt gatctagtgg accagattac gcccttgcat gattgtatcg aagcgatggg
     8221 ctggcctttg ctgattgtgg agggtgttga agcagatgac gtgatcggaa cattggctaa
     8281 acaagcttgc agtcatggga tgcaatgcat tatttcaacg ggcgataaag acattgcgca
     8341 actcgttaat catgcaataa agctggttaa taccatgaat aatgagacgt tggatgcaga
     8401 aggggtcgtt aaaaagtttg gggtaaatcc agcacagatc attgattatt tagcattggt
     8461 gggtgacagt tctgacaata ttcctggtgt tgagaaagtg ggcccaaaga ctgcagtcaa
     8521 gtggttatca cactatcaat ctctggataa tttaatctta cacgccaatg agattacggg
     8581 ggtcgttggg gagaatttgc gtaaagcatt gccatggtta agtgtagccc gtcaattact
     8641 gaccattaaa tgcgatgtat cgctaccggt tgattttgag ggactcatta ctaagcagca
     8701 ggatattccc aagttgattg cgctctatga tgagttagag tttaaaactt ggctacgtga
     8761 actcaagacg agtaataccc ttgcttcagg ggatagggag caaacggaga atcaagttac
     8821 acaacaccaa aacaactcga attcacgagc gaatactaaa cagagcgata ttcattatga
     8881 aacgataacc acagaaaccc agcttgatcg atggataata caatgtgagc aggcgcccat
     8941 tgtttcagtc gatacggaaa cgacgagctt agatccgatg caggcgcgaa tcgttggtat
     9001 ctcattcagt attgaatcta gtaaagcagc ctatattcca ttaggacata cttatacagg
     9061 ggtattcgat cagttaaatt tagattacgt actaaacaaa ctgaaaccct ggctggaaga
     9121 cgcaacaaaa atcaaagtag gtcaaaattt aaaatacgac aaacatgttc tggctaatca
     9181 cggcgtaatg ctcaatggga ttgcgcatga tacgttattg caatcctatg tgctggaatc
     9241 acatcaaccc cataatatgg ataatctggc attgcgccat ctcgatattg aaacgattca
     9301 ctatgatgcg gtaacaggaa agggtgcgag taagatcgga tttgagcaag tggcgattga
     9361 gcaggcaaca cgatatgcag cagaagatgc agatattacc ttgcggttgc accgctatct
     9421 ctatcctaaa attgagcaag atcgttcatt acagttcgtg tatcaccaaa ttgaaatgcc
     9481 attgctcgct gtactttttc aaatcgaacg aaatggcgta ttactggact ctaacttact
     9541 tcatgcgcaa agccgggatc ttgcgcaaca gctttatcat ttggaaaatc aggcttgtgc
     9601 cctggctggt caggtattca atctaaactc tcccaagcaa attcaagaga ttctatttga
     9661 acaactgaag ttacccgtca ttaaaaagac gccaaaggga gccccttcta ccgatgaaga
     9721 tgttttgcag cagcttgcat tggattatcc tttgcccaaa gtattgttgg attacagagg
     9781 gctggccaag cttaagtcaa cgtataccga taaattgcct caaatggtca attccaatac
     9841 aggacgtgtc cataccaatt atgcccaggc agttgcagtc acagggcgat tggcaagcaa
     9901 tgatcccaat ttgcaaaaca ttcccattcg aagtccagaa ggacgcaaaa tacgggaggc
     9961 attcatcgcg ccggaaggga gtcaaatcat ttctgcagat tattcccaga tcgagttgcg
    10021 aattatggcg catatctcac aagatgcggg gctactgaaa gcatttgctg cggatgagga
    10081 tattcaccaa gcgactgcgg gagaaatttt tggtgtaccg attgagcagg tgaatgccga
    10141 gcaacggcgc tacgcaaaag taattaattt tggtttaatc tatgggatgt cagaatttgg
    10201 tttggccagc caactcggga tcgaacgtag tgccgcccgt gtttatatcg aacgctattt
    10261 tgcacgttat ccaggcgttg ctcagtatat gcagcagacg cgagagcgag cgagagaaaa
    10321 gggttttgtt gaaaccgtat ttggtcgccg tttgtggtta ccggaaatta atagtacgca
    10381 tcataatcgt cgacaaggcg ctgagcgtgc ggcgatcaat gctcccatgc aagggaccgc
    10441 ggctgatatc attaaactgg cgatgatcgc agtccataat tggttgatca atcatcaatt
    10501 taagaccaag ctcatcatgc aggttcatga tgaattagtg cttgaagtgc ctgatgctga
    10561 ggtggcaatc gttcaagcaa acttatcgca attgatggaa aatattggta catttgatgt
    10621 tcccttacga gtcaaggtcg ggatcggaaa aaattgggag caggcccatt gatctttttg
    10681 atatgaaaaa atgagtaata aagaacaaat gcaagtttat cgtgagcgcc gtaatcgttt
    10741 acttgatcaa ctgacccatg gtattgctgt gattgcaaca gcaccgatgc gacccagaaa
    10801 ccgagatagc tattatccat atcggtttga cagttatttt tattatttga ctggttttga
    10861 ggaaccagaa gcggtattgg ttgtggttgc agagaatcaa caaggtaacg cagagcagat
    10921 tttattttgt cgtgataaga atttagagcg agagatttgg gatggttttc gttatggtcc
    10981 cgatgctgca tgcgagcggt ttaaatttga cagcacctac ccgatcagca gactcgacga
    11041 aatgatgcct aagttactcg cgaaccaatc aactgtctat acgatgattg gacaagatcc
    11101 agcttgggat acgcaaatca caggatgggt taatcgtgtt cgtgaacaaa cccgaagcgg
    11161 catcactgca ccggatacgc ttgccgatgt gcgcaaaatc ttggatgaaa tgcgcttgtt
    11221 taaagatgag tatgaactta ccctaattcg gcaagccgct aacattgcag tagatgccca
    11281 tcggcgtgca atgaagcatg ctaatcccgg tttatttgaa tatgaaattg aagcagaatt
    11341 aattcatgag tttcgtcgcc aaggcgcatt agcaccagca tataccccaa ttgtagcggg
    11401 aggtgcaaac gcttgtgtac ttcattacat tgaaaataac atgcgtcttc aatcgggtga
    11461 tttattactg atcgatgcgg gttgcgaata tggcgggtat gcatcggata ttacacgaac
    11521 ttttccgata aatgggcgat ttaccgcagt acaacgcgat ctctatcaat tggttctttc
    11581 tgcacaagat gccgctatta aggctgtgca acccggacac acttgggatg atccccatca
    11641 aacagcattg acagtccttg ttcaaggatt cattgatctt gggttgtgtc aaggtagtgt
    11701 tgaaggcatt attgaatcgg aagattataa gcgtttctat atgcatcgta cgggacattg
    11761 gcttggtttg gatgtgcacg atgcgggtga atataagcaa gctggtgcat ggcgcgagtt
    11821 aaaaccaggc atgttgttaa cggttgaacc tgggtgctat atccgtcctg ctgacaatgt
    11881 tcctaatcat ttttggaata acggcattcg gattgaagat gatgtgttgg ttaccaccga
    11941 gggacatgaa gttttgactg ctgctgcgcc caaaacaatc gtggaaatag aggagtggat
    12001 gtatgaaaga agatgaccct agacaaatca ttattagcaa gatcatcgaa catcgagaga
    12061 ataatacatc tcaggctgaa tattcaactt cggtatctcc tgttagtcgt tggttatttt
    12121 acttggcgct tattccagtc acaattatcg tttttttcgt gggctttttc tttttctcga
    12181 ttttattagc tattctattg gtagctatgg cggggattgg tgcaaggatt tggtggttgc
    12241 gacgtaaatt caaacaagca gcgaatgcgc aagaagagat caagtctgca gagatcgaag
    12301 atgctgaaat aattgaaacg gtaattcaca aaacgaataa aaagtagtta gtaatcccgt
    12361 gaatattagt ttggtagcgg tttttattaa ggaggaaatt tcatgaatag tgactcaact
    12421 gctgtacgca aagctgatgc cagtagcgca aatgccttgc aagtgaatat tcggatgcaa
    12481 ccaggtgagc ataccggaca gcctattttt tccaatctaa catcggtgca ggcgagccaa
    12541 ggtgtggttg tgatcgattt tggatttatc gagccacaaa tgattcaagc gttaagtcga
    12601 ttggctcaat ctggcgaaaa agcacctgat acgattagcg caaagatgtc atgtcgtatg
    12661 gtggttagcg ttgaggcagc tagccagtta gctcagcagc ttagccagct ttttgtaaaa
    12721 aaaacatgaa aatgtaaact gaccaggagt gattgtgaac caatctaatc cgctaccagt
    12781 gaagattaag atacagcagg acgagcggtc aggaaagccc agttatgcta attttacgac
    12841 tgtgcaacat ggccaaggca agattgttat tgattttggt ttttttgatc caaagttgtt
    12901 acaagtatct gtcaatgttg ataacaagcc attaggtgta atcgatgctc acttgtctcg
    12961 tcgtattatc ctcagtcaag aggtagcaac ccagtttgct caacaattga gccaactgtt
    13021 tacaaagcac ggagatgtaa aagaaccaca aagtgaatcg gtgatgacgg atactctacc
    13081 gagtgaaata caggaccaca gtcctgaaca aggtgagaat ttatcagaaa atcccaagaa
    13141 ggggttccgc tttccttggt tcaagaaaaa atagtaaaac aggaagggcg atgacttcca
    13201 gtgcttatct ggttatttac gagtattttt attaaaagaa gcgtggtgtt tggtgggtgc
    13261 tggattccgg ttctcaatat ggcggaacgt aattcgtcca ttgctgatat cgtacggtga
    13321 aagttctatg gtaattttgt ctccagccag tatacgaata tgattctttt tcattttacc
    13381 cgcagtgtag gctacaagct ggtgcccatt ttctaacgtg actaaatacc gagaatcagg
    13441 taatacctta tcaacggtgc cattcatttc aataagctct tcttttgcca tcaatttatt
    13501 tcctggaaag gtgttgatta aaaataaaaa gcctggaatg tgtcccaggc ttttatagcg
    13561 ggtcacacaa tttagtgacc cataaagaag ttggtgatcg tacttaagca ggacgtatat
    13621 ttactgcctg cggacctcta ggtcccggcg aagtatcata gcttactcgt tgattttctc
    13681 tgagtgttct aaaaccgcca ccttgaattg ctgagtaatg tacaaataca tcagtactac
    13741 cgttttccgg tgtgatgaaa ccaaatcctt tttcgtcatt gaaccactta acagtgccca
    13801 ttgccatgtt ttatatcctt taaagaaagt tattaagaag aaagggactt attccccaaa
    13861 ggcgaggatc aagatgacaa aggaaagagg gataaaaaaa ccaacaacat ttatatcata
    13921 agaaaattta aaccaaacag ctttaacatt gcttgagaat cgctttccat aatatgcgcc
    13981 ttattgatga gataagcaaa tttattttag gcaatagtcg atcttcataa tggtttgtca
    14041 ggatagcgtt taataaccag cagcaactgc ttgttcaagc cagttagaaa atgtgccaat
    14101 cgagataatc tgattacctg aagcagggcc taagtagtaa acctcaccat tcgctatacc
    14161 cacataagat tgggtagtcg ggtaatagcg atagtaataa ttttcaaacg ttgctgagac
    14221 agtttgacct gagccgagtg gattggccgg ggatagatat tgcgaataaa atgcttctaa
    14281 ataagaaaat aaacgatctg aatcattctc cggtgtatcg gttttgttag aggtgatccg
    14341 gctaacaatg actagatttt tttcctgcaa tttgtgatct tcaatcgtac cgatgatggg
    14401 taatgtcgaa aaaacacttc ctgcattggt tcggggtaat gcagcaatgg catcagccac
    14461 ttgcatgcca ttacccagca cttgtccaaa tactgtaaat ccttcatttt gcttattcag
    14521 atctgcagag ttatcggcga gattgataaa ccattcgctg gtagcgctat tgggtttgtc
    14581 aggcagcttt gccatagcaa tcgtaccgcg cacattggaa cgcgtgacac taaattcatt
    14641 gtcaactggt gatagtttgg caatagtatt gaccgcattc gtcgtttgat tccatacgta
    14701 gcctcctcct tgaatgatga atccaggtat cgtgcgatga aagaaagaac gatcgtaggc
    14761 gttgctcttt aggtattcaa agaaattagc aactgttcta ggtgcggtct catcgtggag
    14821 tttaatatcg atgaccccta gtgttgtttg cagtcgaatg attgtagctg tgcttgtaag
    14881 tggggaaatt aagagaaaaa gaccgactaa tcctggccaa agtcgagtta atcgcaaatt
    14941 attcattatt ctaatgctcc tcttggctta tgctaaaaag ggatatcatc ttccaaatca
    15001 tcaaatcgat cacttttttt ggattgtcca gttggtttat ctgaaaagtt agcgctatta
    15061 tattcgccgt ctttgtcagt aggttcgtaa ctagcaccac cggaacggct tcctagcatc
    15121 tgcatacgat cagcaacgat ttcggtcgta tagcgttcta tattggattt atctgtccat
    15181 ttgcgcgttt gtaatctacc ttcgatataa acttgtgagc ctttttttaa atactcacca
    15241 gcaatctcgg cctgcttgcc aaacatgacg acacgatgcc actctgtttt ttcttgtttc
    15301 tctccttctt tatctttcca agtatccgtg gttgcaatgt tcagatttgc cattgcatca
    15361 ccactcggca tatatcggat ttcagggtca cgccctagat ttccaaccaa gatcacttta
    15421 ttcactgatg ccatgtttat gcgcctcctt ttatcaattg aaccactttt tcttcatcaa
    15481 aaccttttag gtctactttc aaataagcaa cttgttcatt agctaaaacg agtgcctcat
    15541 gaacaccggg taacgctgcg agttcttttg ataatccatt tgctcgctga ttatccattt
    15601 ctgcaacatg atacattctt gagcgaaccg cagcaggcga tttcattgta aaggcgaaat
    15661 aaagccaaac caataacaac attgtacaaa aaatgataat cgctgtgaga ccaacgtgct
    15721 gatataagta tccaccaagg cttgcaccca caaatgcgcc taagaattgt gtgctactgt
    15781 aaataccgat agcggttcct ttggcttcga cgggtgcaat tttagaaata agtgaaggca
    15841 agctggcttc taataaatta aatgctgtaa aaaaaatgac taatgcgata ccgattcccc
    15901 ataaagattg acttgtaaat gcaatcaagc cttgacctat tgttagcaaa gcaattgcag
    15961 tgatgaagac gggttttagc ttagcttttt tttctccata aataatagca ggtaccatta
    16021 ataagacgga tagaacaaga accggcagat acatttgcca atgatgatcg gcagcaagcc
    16081 cggcctcacg aagtgaaaga ggaacaacaa gccaaagcgc cattaaaacc gcatgtaaag
    16141 caaaaatacc ataatttaat ctgagcaatt ctggatgacg gagcgctgtc gagaaacgac
    16201 ttttcgagac ttcagcatcg gaatggaagc ggctgacgat gggatcggga atgattttat
    16261 gaaccacgat aatggcgatt atcgcaagta agcccgtcat aatgaaaata cccggtacac
    16321 caatccattg attgagcaat ggcgcaatca tgagtgaaat tgcaaacgtt gcaccaattg
    16381 tcatgccaat cattgccatg gcttttgttc ggtgttcttc acgtgtgaga tcagctgcaa
    16441 gcgccatgac ggctgcagaa attgcaccag caccctggat tatacgtcct gcgattaccc
    16501 aataaatgtc aacggcggtc gcagcaataa agctaccgat cgcaaataac acgagaccaa
    16561 gataaataac aggtttacga ccgatacgat cggataacca gccaaatgga atttgcaaaa
    16621 tagcttgagt taatccataa gcgcccagtg cgataccaac tagcgtaaaa ttatctccac
    16681 ccggtaaatg ttcagcaaat aatgcaaaaa ccggtaggat aatgaatagg ccaaacatac
    16741 gcagaccgta aacgctggca agaccgaccg aagcgcgctt ttcgagcggt gacattttct
    16801 cagaattggg taacgacgac atgaatctga ttgtgataaa actagaaagt tgggtatatt
    16861 atcaggttga cccatactta gatagccgat ggaactaatc aaaatacgag gggcgcgtac
    16921 ccacaatctc aaaaatatca atctggatat tccccgcaat aagcttgtcg tcattacggg
    16981 tttatcaggg tcaggtaagt cttcactggc ttttgataca ctttatgcgg aagggcaaag
    17041 acgttatgtc gagtcgttat ccgcttatgc ccggcaattt cttcaattaa tggagaaacc
    17101 cgatgttgat ttaatcgagg ggctgtcacc tgcgattgcc atagaacaaa aagcaacctc
    17161 gcataatcct cgatcaactg tcggaacggt aacagaaatt catgattact tgcgcttact
    17221 atacgcacga gttggcgatc ctttttgtcc tgaacatgga attaaactct ctgcccagag
    17281 cgtgtcgcaa atggttgatc atgtcctgcg attgccagct gatactaaac taatggtact
    17341 agcgcccagt gttgtgggac gtaaaggcga gcaacaggat ctgtttgatg aattacgtgc
    17401 ccaaggattt acaagaatcc gactagatgg atcgatttac gagattgatc agcttcctaa
    17461 gcttacaaaa aataaaaagc atacgattga ggtggtaatc gatcggttaa aagtttcttc
    17521 ggaagcaaag cagcggttag cagaatcgtt tgagatggca ttacgccatg gcgatgggcg
    17581 ggcaattgca gtcgagatgg aaacagggca agagcatctt ttttcagcta aatttagttg
    17641 ccctgtctgt aattatgcgc tatcagaact cgagcctcga ctcttttctt ttaataatcc
    17701 cgtaggagct tgtccaaaat gcgatggact gggacaaatt acgttcttcg atcccgcacg
    17761 tgttgtgaca ttaccgcatt tatcgttggc agcaggtgct attaagcatt gggatcggcg
    17821 caatcaattc tattttcaaa tgttgactgc gatcgcgaaa cactatgaat ttgatattga
    17881 aacaccattt gaacaattgg cggacactat caaacagatc attttgtttg gctcgggaaa
    17941 agaaaagatt caatttacgt ttctcaatga aagtggtcgc gcatccaaag aactttatcc
    18001 cttcgaaggg gtgattccta atttatcgcg gcgttataaa gaaactgatt ctcagacagt
    18061 tcgggaagaa ttagctaagt tcattaattc acgtatttgc ccagattgtg agggcacgcg
    18121 gctgtgtcgc gaagctcgcc atataagggt gagtgagcaa gcaatctatc aaattagcgc
    18181 ttggcccctg aaacaagcaa gtgctttttt tgatcaatta gtgttagcgg gccataagca
    18241 ggaaattgcc gaacgcatta ttaaagagat ttccaatcgc ttgcaatttc tgaacaatgt
    18301 tggattagat tatttgtctt tagatcgatc ggctgatacg ttatcaggcg gtgaagccca
    18361 acgaattcgg ttggcaagtc agattggatc agggttgaca ggtgtgatgt atgtgctcga
    18421 tgaaccttcg attggcttgc atcagcgcga taatggacga ttgctcgata ccttgcaaca
    18481 gctacgtaat ttagggaata gcgttatcgt tgttgagcac gatcaagacg ccatttttct
    18541 agcagaccat gtggtagata tggggcctgg ggcaggggaa catggtggtt gtatcattgc
    18601 gcagggtgcc cccagcgaaa ttcaacatca tccagcttca ctaacaggtc aatatctctc
    18661 tgggaaacga tcaatcgcga ttccagctgt gcgaaccccc cttaatccag agcggatact
    18721 aacgctatct ggagtaaccg gaaataattt gaaacaggtt gaacttaatc ttccagtagg
    18781 gttgctggta tgcatcaccg gtgtatcggg atcaggaaaa tccacattga tcaatgacac
    18841 actataccga gcggttgctc agcagttgta tggcaataac acggaacccg caccctatca
    18901 aactttggaa ggcatcgctt ttttcgataa agtgatcaac atcgaccaaa ccccaattgg
    18961 tcgtacgccg cgttccaatc cggctaccta tacaggttta tttacaccca ttcgtgaatt
    19021 gtttgccggc gttcctcaag cccgagaacg tggctataca cccggtcgtt tttcgtttaa
    19081 tgtgaaggga ggacggtgtg aatcctgtca aggggatggt gtcattaagg tagaaatgca
    19141 ctttctgccg gatatttatg tttcgtgtga tgtgtgccat ggcaaacgct ataaccgcga
    19201 aacgttagag attcaataca aggggcggaa tatcaatgaa atcttacaaa tgacggtaga
    19261 aaatgcccat accttttttg agtcagttcc cgtcgtggct cgtaaattgc aaacattatt
    19321 gaatgtcggg ttgggttata ttacgctggg acaatcagcg actaccttgt ctggggggga
    19381 agcgcaacgg gttaagcttt cattggaatt gtctaagcgg gatacggggc gtaccttata
    19441 tattctggat gagccaacca cagggctgca ttttcaagac attgatctcc ttttgaaagt
    19501 actgcatcgt ttgcgtgatc agggaaatac tgtagtggtg attgagcata atttggatgt
    19561 gatcaaaaca gcagattgga ttgtcgatct tggtcccgaa ggaggggatg ggggtggtcg
    19621 aattatcgtt gaaggcacac cggaaagcat tgttgaacat cccgaaagct ttacggggct
    19681 ttacttgaag ccgatactcg cagcacagat aaaaaggtaa ggtatgaatc cacgcgaact
    19741 cctattaaat agctttaaga ttgcgctcga agcggttgat cctttgaagc tcatacccgc
    19801 ccatttaccg gcacctccca aaagaaatac cttagtcgtt ggagctggta aagctgctgc
    19861 agcgatggca caagcggttg aaatacattg gtcacctgat gctaatttag aaggtatcgc
    19921 tattactcgc tacggacatg gtttaccgac acaacgcatc actgtgatcg aagctggtca
    19981 ccccattcct gatcagaatg gcgaaatggc gacacgggcg atcatggaat ctgcccgaat
    20041 acttggttcc gatgatttaa tgctttgttt attcagtgga gggggctcga gtttactctc
    20101 gatgccgatc gaaggtgtta cctttgaaga acttaaagat atcaatcgtc agttaattcg
    20161 gtgtggtgcg tcaatacaag aaattaatac cgttcgtaag catctatcaa atgtattggg
    20221 aggaagactc gctgcagcct gtcaagcgcc gattcattgt ttgatcattt cagacgtgac
    20281 gggagacaat ccaacgcatg tttcatccgg gccgtgctcg ccggatccga ccacctacgc
    20341 ggatgcgctt gcattgatta aacactatgc gatcgatatt tcatccaatg taagcgcaat
    20401 tctgcaacaa cgaagtcaga acccagctga tgaaacgcct aaagcgggtg ataccgcatt
    20461 tagaaatgtg aagagccgtg tcatcgcaac cgcgcatcaa tcgttggttg cagttgaaag
    20521 cttttttcgg gagcataaaa taacgccgat ggtattgggt gatacagtca ccggagaagc
    20581 gcgcgaagtc gctaaggttt acgcagcact tgcaagagaa attcgccatt atggtacgcc
    20641 cttcagacca cctgttgcat tgatatcagg aggagaaacg accgtaacca ttaaaggaca
    20701 agggcgtggt gggcgcaata cggagtttct actatccctt gcaatcgagc tcaatgggtt
    20761 agagcaagtt tatgcactgg cttgtgatac tgatggaatt gatggtactg aaaacaatgc
    20821 cggtgcgatc ttaacaccgg ataccattgc cagagcacag ttgctcggtc tcaaaccgat
    20881 agacttactc aacaataacg attcgtatca attattcgaa tggttgaacg acttggttgt
    20941 gacgggaccg acacgaacca atgtcaatga ccttcgggtg attttgatta catgaaaacc
    21001 tttacaatag gcgctagcca tgacacagat tatgattatt tgctagcagg actattacga
    21061 aattgattga gtagcattgc taactgattg aggtcaaatg gtttgctgag atgctcgtcc
    21121 ataccagcgt cgaggcaatc ttgccggata tgatcgaatg cgtgtgcagt caccgcaata
    21181 atgggaatac gcggttggtt agtttgctgc tccagtctgc gaatatggcg tgtagcctcg
    21241 aatccgtcca tgatcggcat gttacagtcc atcaatatta aatccgggtg ttgattctgg
    21301 tattcagcaa tcgctgcttt tccatcgcta gcaattgcaa tttcacaatt cagatgctcg
    21361 agcattgctc ggctgatcat ctgattcacc acgttatctt cgaccagtaa tactttgagg
    21421 cgtcgatctg tacttttcag ttttactgaa ttgattggtt cgaccgtttt gctttcaact
    21481 tgcgagttaa cttctgtcgc gtgttttaga ggaatacaaa ccttaaattg cgaacccctg
    21541 ccactttcgc ttgtgagtgc gatcgttcca cccattaacg ttagtaaatt ttgggtgata
    21601 gcaagaccta ggccggtacc ttgaatatgt tgtgtcgatt ctcccacttg cctaaagtct
    21661 tgaaatagca gtttctggtc ttcgtgtgaa atcccgattc ccgtatcgat gatcgtaaaa
    21721 tgcaactcaa cgatatcaga ctcaggaata tcaatatatt tgactgaaag ctggacacta
    21781 cctgcgggtg taaatttaaa agcattaccg agcaaattaa ataagacttg cttgatgcga
    21841 tcgggatcac cagtgagtaa ggtaggtaat ggggtatcaa tgtcgatgat aaaatctagc
    21901 cctgtttcat ctgctttagg acgaaacagt aatttgattt cttggatcaa atgcgatgga
    21961 ctaaatgcgc gattgacgat agcgagcttt tgggcttcga ttttagcgaa atcaagaata
    22021 tcatcgatga cacgtaatag cgtgcgccct gagctccgga taattgccag ttgctgggat
    22081 tgcacagcgt taagcggcgt attggccatt agttccgcca ttcctagcac accattcatc
    22141 ggcgtgcgaa tctcgtgact cattgcagct aaaaatgccg atttcgcctt atttgcttgc
    22201 tcagcttgtg agcgagcttg ttcaagatct ttcgtttgtg tttccagtag tgatgcttgc
    22261 tggcggataa tccaataatc atggttttgt agctttccac tgtgaacgca ataaatgaaa
    22321 taggctaaac ccacgaatag gactgtccag ccggaatcaa tgggtaagaa aattgcaagg
    22381 ctaaagaggc aaggcaaaaa gatcaaactt gcaaatgttg tcattagcgc cattcgtggg
    22441 gctgtagcag agatgccgcc agacaaaaaa ccaatcgtta ctgcgatcgc aataagcacc
    22501 ccatcgctaa tctcgttgat tgttgccaga atgatgataa agaaaccaac ccaactaagc
    22561 gcggtaagaa cgtacactgt aaaaataagg cgctcaatca atgcgtctga gaatgtgtta
    22621 agctcaacct gcttaaataa gaatagacgg agcagtgcaa ggataaagaa gcatacgatg
    22681 aaagcaataa tgctcgattg ctcacgcaaa tcgggtgaga aaagtacaat gctgattgcg
    22741 cacagtaaat aaaataaccc tccaaagcgc atgcgccggg ctatatcgtg gttcgtgcta
    22801 gaccgtgctt tgtcgagata ggcgatggca ggatcggacc acataccaat gttgcagtga
    22861 aataattaag aagaatcgga taacactaga taattcgtcg atattgaatg acttcggcga
    22921 tatatttttt attcatttta ttgtctcccg atcaatcaac tatagtgcgt accattttca
    22981 aaagtgatgg tatgcgcttg cctataagtt taactgattg atcgcttgct tcaataaatt
    23041 cttactggtt gtatcaagga tgcagaggtg accggtttct tttacattca aactgatatc
    23101 agggggttca tcatgtcaat gcaatcgatc aatccggcgg caggccaaat cacaccatcc
    23161 ttttcgatat ggcaggctga tcaaatcaat actgcattgg atcaagtcga atcggcgcaa
    23221 gcaagatggg cggcgctcag ctatgagcag cgcggtcaat gcttccggcg attggcacac
    23281 gtatttcggc aaaacagcga aagctatgcg catctgatca ccgaagaaat ggggaaatta
    23341 ctcagggaag cgcgcgccga gatcgagaaa tgtgccatgg gttgcgaaca ctatgcagat
    23401 catgcagcat cgtacctgcg tgatgaagtc atcgcatctg atgctagccg cagtatcgtc
    23461 gtttatcagc cattaggcac tattttgtgc atcatgccgt ggaattttcc gttctggcaa
    23521 ttaattcgcg ctgcggcgcc agcgatgatg acgggtaaca cagtggttct gaagcacgca
    23581 tcgaatgtgc cgcaatgtgc gcttgcattg gaagcagcat ttcaacaagc gagattcccg
    23641 actcatactt ttcgcacact catgatcagt gctgcccaag cagaacaagt gattgccgat
    23701 cgccgcatcg cggcagtgac gttgacgggc agtagtgctg cggggcgcaa agttgcagca
    23761 attgctgggc agcatttgaa aaagtgtgtg ttggaattgg gaggatcaga tccgtttatc
    23821 gtgcttgacg atgctgatat cgaatggact gcgcagcaag cactaatggc ccggttgcaa
    23881 aatatggggc aaagttgcat tgctgcgaag cgctttattt tgctggaaac gatcgctgat
    23941 ggttttgtag agcggttgca agcacttatc agcgagttac gcagtggcga ccccaaagat
    24001 gccgcaacca ccattgcacc gatggcacga gtggatttgc gcgacgaatt gcatgcgcaa
    24061 gtagagcgaa gcctggcatt aggcgcaaag ttacgagccg gcggtttttc cattggtgag
    24121 cagggcgcct attacgcacc aaccattctg gatcacgtgc aacctggaat gccggcattt
    24181 gacgaagaat tattcggacc tgtcttcgcc attgcgcgtg tcagtaatgc cgaggaagca
    24241 ataaaattag cgaatcaaac ccaatttggc ctcggtggca gtgtatggac aggcgatctg
    24301 gcacgcggtg aagcactggc acggcaaatt catgccggtt gcacattcgt caatggtatc
    24361 gtcaaaagcg acccacgctt gccattcggc ggcatcaaag attccggcta tggcagagaa
    24421 ttgtcctatc acggtattcg tgagttcgtg aatatcaaaa cgatttggat taggtaggtg
    24481 ttttaggggg tgtaatggtt acgttggagg aggccgatgg tttcaagtca gagcatccac
    24541 gctcgaaaaa cttgccaaaa ctgagtgcgc agttatttcc tcggtcgcgt gtaaataacg
    24601 cgtcctactc ggtcgatatg ggcgatcagg tcggtatttt caagtaggcg gtattgcgac
    24661 aaatcgacgg tataaggcaa catcaggtcg tcaatctggt cttcaatttg catgaactcg
    24721 gcaaatgaaa tctcgctgcc tttaattgtc agatcgatat cggagcctcg gcgataattt
    24781 cctttcgctc ttgaaccata gatcaacact gaatcgatgg tgtcgtgtct tgcaaatacg
    24841 cgattgagtt tttccaaggt ggtatgtgac aaaccaaatg aatactgctc gttcatggct
    24901 ggtatttttc tatttctttt ttcagtgccc tgaataaagg atagtaatca tgaatgattg
    24961 cattcaccaa ctgctcagcg gtgtgctcat tgtaggtatg tgaggagatg ttgcggctct
    25021 ggatcatggc catccattgc tcgccatctt gaatcaactc aactttaaat gcttcacgag
    25081 ttgcgtcttt tgagcctctg acattctgat tgccttgatc ctgcaaaaaa tctttcaata
    25141 gattccaagc caattcgtgg gtaaattcga atgcttgaat aacaccttgt ttttccaatg
    25201 ggctgagtgc ccgttgattg ctgagctcaa ccgcattttg caattgcagc aacgcttttt
    25261 gataattggc aaaccgttgt tgccagcgga tatctggatc attcattgga ttttctctgt
    25321 gtttgaatca atgcgatgcg gattaagatc ggataataga gcaagtttta tttaacagat
    25381 tttactgttt taagggcagt ttttaaaaaa cggatttaac gcactgtttt cacattaagt
    25441 tatttttagt tcatcttaac cagatagttg ttaatgctta tccagtctac gatactgaat
    25501 tgcttccgca atatgttgat gattaattct ctcagcattc cccaagtctg caatggtgcg
    25561 tgccaatttt aaaatgcgat gataggctct tgcagatagg cttaatcgat tgatcgcttg
    25621 cttcaataaa ttctcgctgg ctgtatccag cacacagagt cgatcaattt cctttacact
    25681 caagcgacta ttggtttttc cctggcggtc atattgcaat tggtgtgctt tctcaacacg
    25741 ttcgcggata atgttacttt tctcaccgat tgactgcgat tgcatcagat cattctgtgg
    25801 caacgaagga acctcgattt gcatgtcgat acgatcgagc agcgggccag aaattttgcc
    25861 ccgatagcgt gcaacttgat cgggtgtaca gtggcatttt ccagaaggat gacctaaata
    25921 gccacagggg caggggttca tggcggcaat taatagaaaa cgcgctggga aattagtttg
    25981 ccgcgctgcg cgtgagatgg tgatatggcc tgattctagg ggctcccgta gtacttctag
    26041 gacttttcga tcaaattcgg gtaattcatc gagaaagagt acgccattca gcgccagtga
    26101 aatttcaccg gggcgcggta tgctaccgcc tccaaccatg gcggttgctg aagcggtatg
    26161 atgcggtgac cgaaaaggac gacgtttcca gtgcgctatt tcaaactgac cttgagcaag
    26221 cgattgaatc gcggctgttt ctaatgcttc ttgctgactc atggttggta agatcgttat
    26281 cagtcgctcc gctagcatcg atttgccagt tccaggcggg ccgaccatca agaggctgtg
    26341 attccctgcg gctgcaattt ccaacgcacg tttggcctga acctgacctt ttacatcttg
    26401 taggtcgatt gtgagcggtg aattcattga atgcgtatca ggggttatcg tgctggaata
    26461 acgttgcagt ggggtttcat tacaaagatg cgcacaaatt tctaggagcg atgcagcagg
    26521 gtagacgata gaatcgctaa ctaacgccgc ttctacagca ttttgtttcg gtaatacaaa
    26581 actgcgacct gaacgtgatg cgctataagt catggcgagc gcgcccttga tcgggcgcaa
    26641 ttcaccgctt aacgaaagtt caccgaccca ttcaaattga tcgagttgat cgtgaggaat
    26701 ttgtgcggtt gctgccagaa ttcctaatgc aatgggtaaa tcaaagcggc cactttcttt
    26761 gggtagatca gcgggagcta gattgacagt aatgcgttgt gcaggaaatt tgaaatggct
    26821 attttgtaag gctgcccgga cccggtcttt gctttctttg acttctgttt caggtaaacc
    26881 aacgatggta aaactcggta aaccattgga taggtgtgtt tctaccgtga cgagtggcgc
    26941 atccattcca gaaagggcgc gactatataa gatagccaga gacatgttat accgcgctca
    27001 atactgaaat acttagttta tgctgggaat gacttattag tgaggagatt tattccaaat
    27061 atcggctgct taaccatcat aattgctatc ggaggttacc gttccagttt ctgaaggcga
    27121 aggttttaat tgaccttcta acgctgctac tttggtttct aactcagtca acttagtacg
    27181 ggttctttta agtacctctt gctgcacatc aaattcttca cgcgtgacga gatcaagctt
    27241 agagatcata ctcgtgagga tcgtgcgtaa gtttttttca atatcctttg ctggaccatg
    27301 cgcgataact tcattaattt tcgtgccgat ttcatccaga atgtttttat ttagcatgat
    27361 atctccatta agcgtctttc aaatgcatca gtatttgtcc agatacgaag cataccttat
    27421 gcgaggtttc caatccaaac atttctcatc attatctaat tccaattaca gatagaaaca
    27481 gtaataatat agcctttacc ttttgtagag gccctgatgg cacggaaggc aagtgggacg
    27541 aagcaac
//
LOCUS       DAOQKQ010000009        26968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_727_length_26968_cov_5.914428, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000009 DAOQKQ010000000
VERSION     DAOQKQ010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 26968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26968
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_727_length_26968_cov_5.914428"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            283..588
                     /locus_tag="PKZ49_01150"
     CDS             283..588
                     /locus_tag="PKZ49_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HND35197.1"
                     /translation="MKKKLWLLSMVGVLALVSGCSDKYTPSASATPESIFKEACVKCH
                     SPVNGKVMALSPEMANTAAIIERIKNGKGFGMPAFPNLTGETAEGLAQYILDNSVTK"
     gene            648..1376
                     /gene="fnr"
                     /locus_tag="PKZ49_01155"
     CDS             648..1376
                     /gene="fnr"
                     /locus_tag="PKZ49_01155"
                     /inference="COORDINATES: protein motif:HMM:NF008365.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HND35198.1"
                     /translation="MSALKKYIASQAISKCASCSLRELCLPVGLDAHETEVLSDLIDH
                     KYKIQRGEYLLHAGMRFKSLYAVRNGFFKSYILDSEGRQQVTGFYMTGELLGLDAISA
                     EIHTCDTVALEDSEVCEVPFERLEEISCSIPLLMHQFHKIMSREIVQDHRVMVLLGNM
                     KAEERIAAFLLNLSERFVKRGYSATSFVLRMTREEIGSYLGLKLETVSRSFSKLQDEG
                     MITINNKHVELHDIHRLTRKISSY"
     gene            1595..2320
                     /gene="rpsB"
                     /locus_tag="PKZ49_01160"
     CDS             1595..2320
                     /gene="rpsB"
                     /locus_tag="PKZ49_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HND35199.1"
                     /translation="MSVTMRQMLEAGVHFGHQTRFWNPKMSQYIYGERNKIHIINLEK
                     TLVMFNEAMQYLRNVTAKSGTVLFVGTKRQARDILSEEAIRCGSPYINQRWLGGTLTN
                     FKTIRQSIKRLQDMEASVQNGILDKLTKKEGLDFQRQLEKLNNSLGGIKDMRGLPDVM
                     FVIDVGYQKGAIVEANKLGIPVVGIVDTNHNPAGVQYLIPGNDDSSQAIRLYARAAAD
                     AILEGREQSIQEIVEMSGEAAES"
     gene            2380..3276
                     /gene="tsf"
                     /locus_tag="PKZ49_01165"
     CDS             2380..3276
                     /gene="tsf"
                     /locus_tag="PKZ49_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HND35200.1"
                     /translation="MADITAGMVKDLRELTGLGMMECKKALVETNGDMKAAEDLLRIK
                     SGAKASKAAGRIAAEGVIGAYVSADQKQGGLVELNCETDFVAKNTDFINFAKALAQLV
                     ADKKVNNADELSSMVLPEGETVEAFRQSLVMKLGENISIRRSACYTAKGKLATYLHGT
                     KIGVMIDYSCEDEVLGKDLAMHIAASKPLCVSSDQVPHDLVERERQIFKAQAAESGKP
                     ANIIEKMVEGRVSKYFAEVTLLGQPFVKDPDQTVEKLLAAKSGKVHAFTLFVVGEGIE
                     KKSGDFAAEVMAQVSQMKENKS"
     gene            3291..4010
                     /gene="pyrH"
                     /locus_tag="PKZ49_01170"
     CDS             3291..4010
                     /gene="pyrH"
                     /locus_tag="PKZ49_01170"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HND35201.1"
                     /translation="MATITYKRILLKLSGEALMGDDKYGINRDVVEHIVIEITGAIQL
                     GVEIAIVVGGGNIFRGVKSAGAGMDRVTADYMGMLATLMNALALQDALSRSGIISRVQ
                     SALRIDQVVEPYIRNKAIRYLEEGKVVIFAAGTGNPFFTTDTAAALRGMEINANIVLK
                     ATKVDGIYSSDPKLNSDATRFKSLSFDEAITKNLQVMDATALTLCRDQNLPIGVFSIF
                     KVGALKRILLGEDEGTSVNNR"
     gene            4030..4587
                     /gene="frr"
                     /locus_tag="PKZ49_01175"
     CDS             4030..4587
                     /gene="frr"
                     /locus_tag="PKZ49_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HND35202.1"
                     /translation="MIADVKKAAEQKMQKSLEALKTDFGKVRSGRPHTGLLDQVLVDY
                     YGTPTPISQLANITLPDARTIGVTPWEKKMLSPIEKAIRDSDLGLNPMTTGDMVRVPM
                     PPLTEERRRDLTKIVKNEAEAARVAMRNTRRDANAQLKELLKDKKISEDDDRKGQDDI
                     QKLTDRYIVEVEKLLQTKEAELMAV"
     gene            4592..5392
                     /gene="uppS"
                     /locus_tag="PKZ49_01180"
     CDS             4592..5392
                     /gene="uppS"
                     /locus_tag="PKZ49_01180"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HND35203.1"
                     /translation="MPRNPSSTREIPVTNDTPRHIAIIMDGNGRWAKKRFLPRVAGHK
                     HGVEAVRAVIKACIERKVGYLTLFAFSSENWRRPEEEVKFLMQLFLGALEQEVTKLNE
                     NNICFKVIGDLSRFDPKIIEFIRQGEKLTANNTRLIFTVAANYGGRWDIMQAIRKMMI
                     QLRDLNENFEEEDLSQYFSMSYAPEPDLFIRTGGECRISNFLLWQLAYTELYFTETLW
                     PDFNAHELDLAIQSYQQRERRFGRTSEQLQDRTSVDKIRPKIAIKNKV"
     gene            5444..6271
                     /locus_tag="PKZ49_01185"
     CDS             5444..6271
                     /locus_tag="PKZ49_01185"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HND35204.1"
                     /translation="MLKLRIITAICLLLFFLSALFFLPPILWGMLLLGMAIVASREWC
                     TLGQFNTKQTLVYLIITTILGGELLFVLSEVVDVNPITTDIVWPYAVSLVFWVLIVPF
                     LLTLSKPARNTAMMMLIGWLVLLPTLLAMYQLRTIDPMILLIFMSVVWISDTAAYFVG
                     KKFGKHKLAPQISPGKTWEGVMGALLAVLCYAMALGYYLEETISFVIMLTFAVIGLAI
                     LGIIGDLFESQLKRQVGIKDSGTILPGHGGVLDRIDALTSTLPTATLIYLIYSHVTL"
     gene            6268..>7139
                     /gene="dxr"
                     /locus_tag="PKZ49_01190"
     CDS             6268..>7139
                     /gene="dxr"
                     /locus_tag="PKZ49_01190"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HND35205.1"
                     /translation="MTTVRKITLLGSTGSIGENTLDVVARHADRFQVCALTANKSVEK
                     IFLQCQRFHPQYAVMLDHKSAAALENKLKAVGLDTQVLSGVESLEKVASLPEVDIVMA
                     AIVGAAGIRPTIAAARSGKSVMLANKETLVMAGRIFIDIVKQNQATLLPIDSEHNAIY
                     QSLPKYFDGNLTTAGVRRILLTASGGPFRRFSERELEHVTPEQACAHPNWVMGKKISV
                     DSATMMNKGLEVIEAHWLFDAAPEMIEVVIHPQSVIHSMVEYIDGSVLAQLGNPDMRT
                     PIAHALGFPDRMESG"
     assembly_gap    7140..7239
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7240..7610
                     /locus_tag="PKZ49_01195"
     CDS             <7240..7610
                     /locus_tag="PKZ49_01195"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112266.1"
                     /note="catalyzes the formation of 2-C-methyl-D-erythritol
                     4-phosphate from 1-deoxy-D-xylulose-5-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HND35206.1"
                     /translation="MRTPIAHALGFPDRMESGVKSLNIFEIARLDFEAPDLGRFPCLR
                     LAYQALAKGGNMPAVLNAANEVAVESFLTGRMSFTAIPDMIEHVMSSVSSTEIDTLDD
                     VLAADSTARLVAQEWLLCKV"
     gene            7678..9045
                     /gene="rseP"
                     /locus_tag="PKZ49_01200"
     CDS             7678..9045
                     /gene="rseP"
                     /locus_tag="PKZ49_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HND35207.1"
                     /translation="MTILTTIIAFIVALGVLITFHELGHYWVARWSGVKVLRFSIGFG
                     QPLFKKSIGKDQTEWVLAALPLGGYVKMLDEREGEVLPHELPRAFNQQSVYKRFAIVA
                     AGPIANFLLAILLYWILFMLGVNGVKPVIGPIKQATPAAFAAFEEGEIIQKIEGESVS
                     TWQDARWALLTQAVDRNPVVVVETVNSHGQTALRKLDFSQIHTDDLDANFLEGIGFIA
                     HQPSMDPIIGQVIAGGVGHQAGLMEGDEILAINQKEILKWEELVREIRTNPGQLIELE
                     VLRKGQIIDVMITPESVNENGQKVGKVGIAPKVDPSMVEKYLINVSYSPIVAFYKACW
                     KTWEMTAFTFRMLGKMLIGEVSWKNVSGPITIADYAGQSAQMGVASFLGFLALISVSL
                     AVLNLLPIPVLDGGHLMYYLIEIIRGAPLSEKTMALGQQIGMALLFTLMIFAVYNDIY
                     RLVAS"
     gene            9048..11462
                     /gene="bamA"
                     /locus_tag="PKZ49_01205"
     CDS             9048..11462
                     /gene="bamA"
                     /locus_tag="PKZ49_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HND35208.1"
                     /translation="MKFSFFLIFLGLFYASTLSAFESFIVRDIRVEGIQRTEAGTVFS
                     YLPVKVGEVLDETKATSAIKTLYTTGFFKDVKLKADNGVLIVQLQERPAIAEITINGS
                     KEFEKDKLKEGLKQAGLAESRIFSRSLLDKAEQELKRQYISRGKYAVKITTTTTPLER
                     NRIGINFDVEEGRTTRIKKINIVGNEVFKDSDLINQLTLTTPGLITWFTKNDQYSKQK
                     LSADLEVLRSYYLDRGYLEFSIDSTQVSITPDFKDIYITINVTEGPQYTVSDIKMAGN
                     FLVPEEELRKLIKLEAGGVFVRERLTESIKLITDRLGDEGYAFANVNASPEIDKEKRE
                     TTFTFYIDPGRRVYVNRINITGNDRTRDEVIRREFRQMEGAWHSTSQINRSKQRTDRL
                     DFFTEVNVETPPVANKPDQVDININVKEKPTGAIMFGAGYSDLEGIILNGSVAQNNIF
                     GTGNFLSLSVNTGRINKVIAASWTNPYWTINGVSLGVDVFRRDINTAALTNVGVFNTD
                     TAGATVRFGIPIAENDIVSLGVGGEYTHIDLKNNSPQRYKDFVEGTQDRFDDKGNLIK
                     GTKGFGSSVTNLPLTLQWARDRRDSAIWTTSGTTHRLFGEFSAPFAEMNYYKVSYEQK
                     WFFPISRLFTVMVDGEIGFGEGYAGDPFPFFKNFFAGGFNSVRGYNINTLGPQEQLLN
                     LQGEPIANRFAVVGASKRAVGTIELLFPAPFMRDEKTVRLAAFFDGGNVANSWSDFLD
                     FNNDTGPFANISYFRFSAGLAVTWISPMGPLRVSIAQPLNPRDRDPFHRGDDIQRFQF
                     QMGQQF"
     gene            11584..12048
                     /locus_tag="PKZ49_01210"
     CDS             11584..12048
                     /locus_tag="PKZ49_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HND35209.1"
                     /translation="MKIGVVNTEKILRESAPAVRAQKKIEKEFLTRDEQLKKVAAQVR
                     EYQDQLERNNKGITDEERRVKERELASLTRQYQRTQQQMREDLNARQNEEYGLILERV
                     NNAIKIIAEKEGYDLILQLQDSVYRSQRIDITNQVIEVLIEQDATLPTQSAK"
     gene            12082..12531
                     /gene="fabZ"
                     /locus_tag="PKZ49_01215"
     CDS             12082..12531
                     /gene="fabZ"
                     /locus_tag="PKZ49_01215"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HND35210.1"
                     /translation="MSTVMDIHEILKYLPHRYPLILVDRVIDLEPNVRILAFKNVSIN
                     EPYFVGHFPHHPVMPGVLIVEALAQAAALLTLKSVKAENDKNQVYYFVGIDGVRFKKP
                     VMAGDQLMLTVEITRQIKGVWKYQAHADVDGQRVTDAELMCTARAID"
     gene            12558..13130
                     /gene="rnhB"
                     /locus_tag="PKZ49_01220"
     CDS             12558..13130
                     /gene="rnhB"
                     /locus_tag="PKZ49_01220"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HND35211.1"
                     /translation="MMYEAAWVCGVDEAGRGPLAGPVYAASVILGADCSIQGLKDSKL
                     LSEKKCQSLTLLIKEHAKAWSIASASVAEIDQLNILQASLLAMKRAIEALPIMPKIAL
                     VDGKHAPRLNCEIRTIVNGDRLIPEISAASILAKTARDAEMIALHERFPVYGFNQHKG
                     YPTKAHFEAIQRHGICEIHRRSFSPCRCFN"
     gene            13263..13745
                     /locus_tag="PKZ49_01225"
     CDS             13263..13745
                     /locus_tag="PKZ49_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HND35212.1"
                     /translation="MLIDKDTVVSLHFFKLTNQEGEVIDESSTPVSYLHGGYGSMFPS
                     VELALQGKEAGYDLSLLMEPEDTFGEYDSELIRVEPRNAFPDNVQKGMQFEGSANNSE
                     DQMVYTVIDVTDDTVVVDGNHPLAGIALKFECSVMDVRAATLEEISHGHVHGPHGHHH
                     "
     assembly_gap    13749..13848
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14157..18695)
                     /locus_tag="PKZ49_01230"
     CDS             complement(14157..18695)
                     /locus_tag="PKZ49_01230"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HND35213.1"
                     /translation="MFSEENEMTTGEFLIQFFPEITLAEQVLLLEQNNAEFIKPIMPT
                     NPNLVLAKVKDPASLDFTLNSLSNHSNVRFVEQNSVVKVESTSDDSYYTNGNLWGMYG
                     DLSTPANIYGSQASEAWNTGFIGTTKTVLGVIDTGIDYTHPDLFQNIWINQKEISTTL
                     RGSLTDVDSDGVISFVDLNHAANVTYVSDLNNNSRIDAGDLLADSRWEDGNDLDSNGY
                     IDDIVGWDFVNNDNNPMDDAQHGTHVAGTIGAMGGNGTGVVGVNWNIQMIPLKALGPS
                     GGTSLTTTAAIDYYTNASLAVGTGQNFVGTNNSWGGSGYSQSNYDAVVRTALAGNLFI
                     AAAGNSSLNTNTSANYPSNFNTTAAAGFDAVVSVAALTSSGALASFSNYGNVTVDLGA
                     PGQSIYSTVPGNSYAALQGTSMAAPHVAGAVALYASALPSATPQQILNALLSSVTPTS
                     SLATTTVTGGRLDIGTLMAGVTPPTGVTVAGGTYTGTTANNIMNGGAGNDILYGLAGN
                     DKLNGLAGNDILEGGAGSDTLNGGEGTDIYLFSLATDHKAAEVFDYGTTGIDEVRFTA
                     TTSGTLTLYAGDVGIEKVVMGTGVAANAVTSGTTVLNVNSSTVTNSLQIIGNAGINTI
                     TGTSYVDQLDGMDGADIYIINSAIHHSAAEIADSGTTGNDEVRFSALVPGTLTLYSGD
                     TGIERVTLTGTAALNINAATVGNGLTILGNAATNVLVGTNFVDSLNGGSGSDIYLINS
                     ASEHSQAEISDSGTSGIDEIRFATTSGNILTLYAGDVGVEQVVIGTGMNSTADTSGTI
                     SAGIDASLAVKGLSLIGNAGSNSLKGSAFNDVIFGGLGVDTLLGHEGSDIYIMESSTD
                     HSSAEINDSGNVGVDEIRFTATTTGTLTLFSGDTGIEQIVIGTGTAATAVTTGTTALN
                     VNASALLAPVMITGNAGNNILTATSGNDTLNGGSGNDVLKGGEGSDIYLISAAIDHGV
                     AEIEDNGSNGVDEIRFSASIASTLTVFSGDIGVERVVIGTGTASNAISSGTAALHVNA
                     SSAANALTIMANAGNNSITGTAFNDVIDGKAGADILDGRNGADIYLISTDAEHKLAEV
                     ADTGTSGIDEIRFASIVAGTTLTLYAGDTGIERVVIGTGIESVANTTAMTSLNVNANA
                     SSNALIIIGNAGDNLITGTAYHDQLNGGNGSDIYIVSTATHHAQAEIADTGNVGTDEL
                     RFAATIASTLTLFAGDTGIDQVVIGTGTKTSAITTGTVALNINASAVTNSLRLIGNNG
                     PNTLSGTAYADILDGRSGIDSMNGGNGADIYIISTSAEHTTAEIADSGVSGVDEIRFA
                     ATAASTLTLFAGDTGIEKVVIGVGLADTAITTGTAAIKIDAAAIGNSLTIIGNNGANT
                     LIGTAFADVFDGNNGNDILTGGAGADKFVFSSQPNSTSNKDTITDFQSGSDVLHFSLN
                     VFTGLGTNPGNLASEQFWSAANAVTAHDADDRLIYNTTSGGLYYDQDGLGGNPAIQIA
                     LLGTTAHPSLTHSDIVLI"
     gene            19168..20382
                     /locus_tag="PKZ49_01235"
     CDS             19168..20382
                     /locus_tag="PKZ49_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HND35214.1"
                     /translation="MNGIQKYFLVLTSLMLVSNLSFASTSNCTQYSTSAIYIASTGLV
                     CLQQVKVIDQSSIQLYEASLQWLGSDNPNQFSLVHADLDTTPEQLSIPSFSAENGMLA
                     IPKVDIPRQFGTERYSVNLTLAPNSDSYLFNLDSISVYINPDHVPRVTWKPYGMLNSN
                     ERRAVDALGMSLPYARLSDAIYDFDNTAVDQWDLIDKISRDSGMQAGVYQHRETFELA
                     LAFRGTETCDFPCSLDETKEYLRDLATDTALTFGFDDAQFKHALNYGKDIVNRFPNRS
                     ITLTGHSLGGGLAQAVGAVLGLKTFAFNSAPVPDDFFDDNPVSLPESALNELIHVLAD
                     IHDPVSNTDETGKFYINSKHAAPLIQFDFDLKEITPNRLAELDKLRFNRHGITKFFEN
                     ASSLMTIYANGW"
     gene            20422..23013
                     /gene="mutS"
                     /locus_tag="PKZ49_01240"
     CDS             20422..23013
                     /gene="mutS"
                     /locus_tag="PKZ49_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HND35215.1"
                     /translation="MDIESKHTPMMQQYLRIKAQHGDKLLFYRMGDFYELFYEDAEKA
                     AKLLDITLTQRGMSAGEAVKMAGVPHHAAEHYLAKLVKLGESIAICEQVGDPATSKGP
                     VAREVTRIITPGTLTDAALLDGKSDSILVALTCHKSVLGLAWLNLAAGQFWVLETTLD
                     NLLSEIERIRPAEIIIPESFDIPTELKEYGVIRQLPPNQFDFDNAVRNLTTLFRTHDL
                     SGFGCDQLHAALQAAGALYHYLYLTQGTNLTHIAGLKIEHDSAYIRMDAVTRKNLEIS
                     ETLSGETSPTLMSLFDTCATNMGSRLLKHWLHHPLRNDAMIQNRLESVAFLKNNLKGA
                     PHFTRIKQLLRPIADIERITTRIALKSARPRDLSSLRDSLIALPEMAETIANHLNAHL
                     NQLANALIPDANLVKLLRQSLQSEPALLLREGGVIADGFDEELDELRMLHSNCGEFLL
                     QLETREKTRTGIPTLKVEYNRVHGFYIEVTHVHTDKIPADYRRRQTLKNTERYITPEL
                     QAFENKALAAKDKALAKEKQLYEALLEQLTPFITLMQQIARSIAEIDVLSTFAERAET
                     FNYVMPTLTEEASITIQDGRHPVVETQVDQYIANHLNLGASSNDKKMLIITGPNMGGK
                     STYMRQTALIVLLAHCGSFVPANCARIGPIDQIFTRIGAADDLAGGRSTFMVEMTEMA
                     NILNYATPQSLVLVDEIGRGTSTFDGLALAFAIARYLLNKSQSYTLFATHYFELTNLV
                     NESERIANVHLGVVEYQNRIVFLHTLNEGPASQSYGIHVAELAGVPKPIIREAKKILA
                     HLEREANHQNPQPDLFSAISQDETIDSLPLSHPVMSHLNTLKPDELSPREALVELYKL
                     KSLLDEQ"
     gene            23262..24176
                     /locus_tag="PKZ49_01245"
     CDS             23262..24176
                     /locus_tag="PKZ49_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35216.1"
                     /translation="MAVRITPRLVELTYEAALKAFWRKEALRKFLRASHVAETFLATW
                     APEESKRDFLDKTFQELQRTDRGKAVIFEMARYLSEQTTFPDLRNWEDSAQKITDATK
                     AVAELKAWLKTQDEEIRSEKEREEAKTKARDEQQKIQRSLTDKAKLQQRLDALHPAVG
                     TQQGGYDFQNWFYDLLDFCEIQNRRPYVSAGRQIDGSLTFDGTTYLVELKFTGSQADA
                     TDIDSLRSKVDDKADNTMGIMVSISGYSSVALRQASGRKTTLLLVDAMHLYLFLSGTP
                     SFGDIISRLRRHASQTGEAYLAVGKFGG"
     gene            24654..25334
                     /locus_tag="PKZ49_01250"
     CDS             24654..25334
                     /locus_tag="PKZ49_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2064 domain-containing protein"
                     /protein_id="HND35217.1"
                     /translation="MIPDRKQSTLVLVCKRPKMGVSKQRLAVKLGREKTLVIAKALLD
                     CALEDAAEWQGPIVIAPADDADSEWAVSLLPEARQIEVFPQRLGNLGQRLNALDQSLR
                     CVGHTQLVYIGSDAPVLQSVDYIACQQALQHYDTVLIPASDGGVALMASNLHWPELAD
                     LPWSTSQLGKALVVRCESAGQSVALLEQRADVDEFADCVKLISNLNDDPRPARQTLLK
                     RIKSIIDP"
     gene            25341..26027
                     /locus_tag="PKZ49_01255"
     CDS             25341..26027
                     /locus_tag="PKZ49_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HND35218.1"
                     /translation="MPSLSIIIPCYRDHAQVAVLLQQLRMLSALPNEIIVVDAAGDSA
                     CQAICMQYDAQWHHSEPCRGKQLLQGAQVATGDILWFLHADTRVSPNSISSIMQAVER
                     GAIGGYFRFVFDGPPAWPMLILEPAIALRSRFGTPYGDQGIFVRRIAYFKVGGHAPWP
                     LFEEVPLVKGLRNLGHFQPLLDSIWVDSRRWQRDGWWHRTLRNRMLALKFILGASPDT
                     LAKSYHSQSS"
     gene            26039..>26968
                     /locus_tag="PKZ49_01260"
     CDS             26039..>26968
                     /locus_tag="PKZ49_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HND35219.1"
                     /translation="MREVVEAYYGETLTCSADLQTNACCTDEGMPSYLKPLMAKIHPE
                     IANRYYGCGLIAPPLLTGLTVLDLGCGAGRDAYLLSQMVGEQGQVIGVDMTEAQLAAA
                     TQYQSYHRDVFGHAASNVRFLQGDIEHLDELNLADGSVDIIVSNCVINLALDKSAVLK
                     EAFRVLKPGGELYFSDVYSDRRIPAVLASDPILYGECLGGALYWNDFLPLAKQCGFID
                     PRLVSDRPITIGNTLLADKVGNIRFYSATYRLFKLNDLEPACEDHGQSVVYRGSIPDH
                     PHHFTLDKHHRIETGRQFPVCGNTFRMLHDTRFS"
BASE COUNT         7723 a         5319 c         6104 g         7622 t
ORIGIN      
        1 attctcgttt aattatttga gaattttttg agcaactttt cgctagttta gtgaactttt
       61 tctaaagtta agcgataaaa acaatactct ttagtccctt gtattaggtt tttaaggaaa
      121 tgaatacaca ctgaattcta gtgtattacg ttacttggct attcaaaacc ggagcgtgtt
      181 gctttcagaa ggtaatatcc ttacttcatt gtgatacact taaaatgtat aacgaatcag
      241 ttaagttaaa taaataaaga tattcaaggg aggaaaaggc gcatgaaaaa aaaattatgg
      301 ttgttaagca tggttggtgt attagcgctt gtaagtggtt gttcagataa atatacccct
      361 agtgcgagcg caacacccga aagtattttt aaagaagctt gtgtgaagtg ccatagcccc
      421 gtaaatggga aagtaatggc gctgagtccc gagatggcta ataccgcagc aatcattgaa
      481 agaatcaaaa atggtaaagg ttttggtatg ccagcgtttc ctaatttgac gggtgaaaca
      541 gcggaagggc tcgcgcaata tatcctagat aatagtgtaa caaaataaaa aaatatcact
      601 gttttctgag ttcgaattac taactatcca gaaccttgag taataacata tcagctctaa
      661 aaaaatatat tgcctctcag gcaatatcca aatgcgcatc gtgtagccta cgtgagttat
      721 gcttgccagt cggtctcgat gcgcatgaaa cagaggtttt gagcgacctg atcgatcata
      781 agtataaaat tcagcgtggg gaatatcttt tgcacgctgg tatgagattt aaatcccttt
      841 atgctgtcag gaatggtttt tttaaatctt atatccttga ttctgagggg cgtcagcagg
      901 ttactggttt ttacatgacg ggggagctgc taggcctgga tgccatcagt gcagaaatcc
      961 atacgtgtga tactgttgcg cttgaggata gtgaggtttg tgaggttcca tttgaaagac
     1021 tcgaagaaat tagctgcagt attcctttac tcatgcatca gtttcataaa atcatgagtc
     1081 gggaaattgt tcaagatcat cgcgtgatgg ttttgcttgg taatatgaaa gcagaagaac
     1141 gtatagcagc attcttgctc aatttgtcag aacgatttgt aaagcgcggg tactctgcga
     1201 ctagctttgt tctgagaatg acgcgagaag agattgggag ttatctgggt cttaagcttg
     1261 aaactgtaag tcgctcattc tccaagttgc aggatgaagg gatgattacc attaataata
     1321 agcatgtaga attgcatgat atccatcgcc tgactcgtaa aattagtagc tattagggtg
     1381 attgaagcca acgttcattt gttcgttact agctaaatta aagttttgcg ctttcttgag
     1441 tgtcaacaga ggcgtgctat aatgcagcct tagatagatc cgacactagt cgcgatctat
     1501 cttacgattt atttaaatac acacacttgt cgatgtaggg tgctaagcaa tcatgatatg
     1561 aggcaagtgg aggactaacc ctagaggatt ttatatgtca gttacaatgc gtcaaatgtt
     1621 ggaagcgggt gtgcactttg gtcatcaaac tcggttctgg aatcctaaaa tgtctcagta
     1681 catttatgga gagcgaaata aaattcatat catcaatttg gaaaaaacat tggtgatgtt
     1741 taatgaggct atgcagtatc tccgtaatgt tacggcaaag agtggaacag tgctgtttgt
     1801 gggtacgaaa cgacaagctc gcgatattct ttctgaagaa gcaattcgtt gtggttctcc
     1861 ttatattaat cagcgctggt tgggcggtac actcactaat tttaaaacca ttcggcagtc
     1921 gataaagcgc ttgcaggata tggaagcctc cgttcagaat ggcatactcg ataagttaac
     1981 aaaaaaggaa ggtctcgatt ttcagcgtca actcgagaag cttaataata gccttggcgg
     2041 tattaaagac atgagagggt tgcctgatgt gatgtttgtc attgatgtcg gatatcaaaa
     2101 aggagcgatt gtcgaggcta ataaactagg tataccagta gttggtattg ttgataccaa
     2161 tcataatcct gctggagttc aatatttgat cccgggtaat gatgactcga gtcaggcaat
     2221 acgtctttac gcacgtgcag cagcagatgc gattctggaa ggtcgagagc aatcgataca
     2281 ggaaatcgtt gaaatgagtg gagaggctgc tgagagttag agggtatttt ttggtatagt
     2341 ttctataaat aattttgatt ctgggtttgt ggagtagata tggcggatat taccgcaggt
     2401 atggtaaaag atttgcgtga gctaaccggc ttaggcatga tggaatgtaa aaaagctttg
     2461 gtcgaaacga acggcgacat gaaagcagcc gaagatcttt tgcggattaa gagtggagcg
     2521 aaggcaagta aggccgctgg gcgtattgca gcagaagggg tgattggcgc atatgtttcg
     2581 gccgatcaga agcaaggtgg tttggttgag ctaaactgtg agactgattt tgtagcgaaa
     2641 aatactgatt tcatcaattt tgcaaaagct ttagcgcaac tggtggctga caaaaaagtt
     2701 aataatgctg atgaattaag ttctatggtt ttgccagagg gagaaaccgt agaggctttt
     2761 cgccagtcgc ttgtaatgaa attgggtgaa aatattagta ttaggcgcag tgcttgttat
     2821 acagcaaaag gtaagcttgc gacatattta cacggtacaa agattggggt aatgattgat
     2881 tactcatgtg aagatgaggt tttgggtaaa gatctagcaa tgcatattgc cgcgagtaaa
     2941 ccactatgcg tttctagtga tcaagttcct catgatttag tggagcgaga aaggcagatt
     3001 tttaaagcac aagctgcaga atcgggtaaa ccagctaata tcatcgaaaa gatggttgaa
     3061 ggtagggttt caaaatattt tgctgaggtg acattattgg gccagccttt tgttaaagat
     3121 ccagatcaga cagtcgagaa gcttttggct gcaaaatcag gtaaggtcca tgcattcaca
     3181 cttttcgtcg tgggtgaagg gatagagaag aaatcaggtg attttgctgc tgaagtaatg
     3241 gcacaagtaa gtcaaatgaa agaaaataag agttgatctt aaaccaacca atggcaacca
     3301 ttacttataa gcgtatttta ttaaaacttt ctggcgaagc tttaatgggg gatgataagt
     3361 atggaattaa ccgagatgtg gttgagcata ttgtaattga gataaccgga gcgatccaac
     3421 tgggtgttga aatagcgatt gttgtaggcg gagggaatat atttaggggt gttaagtcgg
     3481 cgggtgctgg tatggataga gtgaccgcag attatatggg aatgctcgcc acgcttatga
     3541 acgctttggc tttgcaagat gctttatcac gtagcggaat tatatcaaga gtgcagtccg
     3601 cattgcggat tgatcaagtt gtggagccct atatacgcaa taaggcgatt cgttatttag
     3661 aggaaggaaa agtggtgatt tttgctgcag gaacaggtaa tccatttttt acgacggata
     3721 ctgcagcggc tctaagaggt atggagataa atgcaaatat tgttcttaaa gccacgaaag
     3781 ttgatggaat atattcgagt gatcccaaat tgaactctga tgcaacacga ttcaaaagtt
     3841 tatcctttga tgaagcaatt acaaaaaatc ttcaggtaat ggatgcaact gcattgacat
     3901 tatgtaggga tcaaaattta ccaatcgggg tttttagcat ttttaaagtg ggtgcgttaa
     3961 aaagaatact cctgggagag gatgagggaa cttcggtcaa taatagatga ttgatggaga
     4021 tttcttgata tgatcgctga tgtaaaaaaa gctgctgagc agaagatgca gaaaagttta
     4081 gaagcactta aaaccgactt tggtaaagtg cgaagtggga gacctcacac aggattactg
     4141 gatcaggttc ttgtcgatta ctatggtact ccgactccta ttagtcaatt ggctaatata
     4201 acattgccag atgctcgaac aatcggtgtt acgccttggg aaaaaaaaat gttatctccg
     4261 attgagaaag caattagaga ttctgatcta gggttgaatc caatgactac tggcgatatg
     4321 gtacgtgtgc ccatgccacc gttgaccgaa gaacgaagac gtgatttaac taaaatcgta
     4381 aaaaatgaag ccgaggcagc acgcgttgca atgcggaata cgcgtaggga cgcaaatgct
     4441 caattaaaag agctgcttaa agataaaaaa atctctgaag atgatgatcg aaaaggtcaa
     4501 gatgacattc agaaattaac tgatcgatat attgttgaag ttgaaaaact attgcaaaca
     4561 aaagaagccg agttaatggc tgtttaaata tatgccacga aatccgagtt cgactcgtga
     4621 aattccggta acaaatgata ctccacgcca tattgctatt attatggatg gtaatggtcg
     4681 atgggcaaag aaacgttttt taccaagagt ggctggacac aaacatggtg ttgaagctgt
     4741 tcgtgcagtg atcaaagcat gtatagagcg caaggtgggt tatttaacgc tgttcgcatt
     4801 tagcagtgaa aattggcgac gaccagagga agaggttaag ttcttgatgc agcttttttt
     4861 aggtgcactt gaacaagaag ttaccaagtt aaatgaaaat aatatctgtt ttaaagtgat
     4921 aggtgattta tcaaggtttg accctaagat aattgagttt attcgtcaag gagagaagct
     4981 aacagcaaat aatactcgct tgatctttac ggtagctgca aattacggtg ggcgatggga
     5041 tattatgcag gctattcgta aaatgatgat tcaattacgt gatctaaatg aaaatttcga
     5101 agaagaagat ttgtcgcagt acttttccat gagttatgcg cctgaacctg atttatttat
     5161 tcgtacaggt ggtgagtgtc gaattagtaa tttcttgctt tggcagctag cttatacaga
     5221 attatatttt actgagacat tatggccaga ttttaatgcg catgagcttg atttggcaat
     5281 tcagtcctat caacagaggg aacgtcgttt tggtcgtact agcgagcaac ttcaagatcg
     5341 tacttctgtt gataaaataa ggcctaaaat agcgatcaaa aacaaagttt agatcgatgt
     5401 ttacggctat ttttgattta tattggcggg ttaaagtccg cctatgctga aattacgtat
     5461 cattacagca atttgtttgt tgctgttttt tctatccgca ttatttttct tgcctcctat
     5521 tctctgggga atgttattgc taggaatggc aatcgttgct tctcgagaat ggtgtacgct
     5581 aggtcaattt aatactaagc aaacgctggt gtatttaatc atcacgacga tattgggtgg
     5641 tgaattactt ttcgtcttaa gtgaagtggt ggatgtaaat cctataacaa ctgacatcgt
     5701 gtggccttat gcggtgtcgc tagtgttctg ggttttgatc gtgccattct tgttaacctt
     5761 atcaaaaccc gccagaaata cagcgatgat gatgttaatt ggatggttag ttttgcttcc
     5821 aactttactg gcaatgtatc agttaagaac aattgatcca atgattctcc ttattttcat
     5881 gagcgtagtt tggatatctg atacagccgc ctattttgtg ggaaagaagt ttggaaaaca
     5941 taaattggct cctcaaatca gtccaggaaa aacttgggag ggtgttatgg gagctttgct
     6001 tgcggtttta tgctatgcaa tggcactagg ttattatcta gaagaaacca tcagctttgt
     6061 tattatgctg acttttgctg taattgggct ggctattctg ggaatcatcg gcgatctgtt
     6121 tgaatctcaa ttgaaacgtc aggttggaat caaagatagt ggaacgattt taccagggca
     6181 tggtggtgtt ctggatcgaa ttgatgcact gacatcaacg ctacctacgg ccaccttgat
     6241 ttatcttatt tattcacacg taacattatg acaacagtca gaaaaataac cctattggga
     6301 tcgactggga gtatcggtga gaatacactc gatgttgtgg caaggcacgc tgatcgattt
     6361 caagtttgcg ctttaacagc aaataaaagt gttgaaaaaa tatttttaca atgtcaacgt
     6421 tttcatcccc aatatgcagt aatgcttgat cataaaagcg ctgcagcgtt agaaaataag
     6481 ctaaaagcgg ttggtctcga cacacaagta ttatccggtg ttgagtcctt agaaaaagtt
     6541 gcgtcactcc ctgaagtcga tatcgtaatg gcagcaatcg tcggtgcggc agggataaga
     6601 ccaacgattg ctgcagcacg ctcaggtaaa tcagtcatgc tggcaaacaa agaaacgttg
     6661 gtcatggctg gtcgtatttt tattgatatt gtgaagcaaa atcaagcgac cttattaccc
     6721 attgatagtg agcacaatgc gatatatcaa tcgttaccta agtattttga tggaaatcta
     6781 actacggcag gagtaaggcg catactactg acagcttcgg gaggcccctt ccggcgcttt
     6841 tctgaacgtg aacttgaaca tgttacgcca gaacaagctt gcgcccaccc taattgggtc
     6901 atgggaaaga aaatctctgt cgattctgcg acaatgatga ataaaggatt agaagtcatt
     6961 gaggcacatt ggttgtttga tgctgcaccg gaaatgattg aagtcgttat tcatccacag
     7021 agtgtcattc actcaatggt ggaatatatt gatggctcag tacttgccca attaggtaat
     7081 cccgatatgc gaacacctat cgcgcacgca cttggttttc cagatagaat ggaaagtggn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tatgcgaaca cctatcgcgc
     7261 acgcacttgg ttttccagat agaatggaaa gtggtgtgaa gtcacttaat atttttgaaa
     7321 tagctagact cgattttgaa gcgccagatc ttggccgttt cccttgtcta cgccttgctt
     7381 atcaggcttt agctaaaggt ggcaatatgc cagcggtact caatgctgct aatgaggtag
     7441 cggttgaatc tttccttaca ggaaggatgt cttttactgc tattccagac atgattgagc
     7501 atgtcatgag tagcgtgagt tctactgaaa tagatacact cgatgatgtg ttggctgctg
     7561 atagcactgc gcgattggta gcgcaagaat ggttactgtg caaggtgtaa catgttttct
     7621 gcctgcgcgt ataatgaatg gctagagtaa gggtatacaa tttttgacta attcgatatg
     7681 acgatattaa caactatcat cgcttttata gttgcattag gcgtgttgat tacgttccat
     7741 gagttgggtc attattgggt ggcgcgctgg agcggtgtaa aggttttaag attttccatt
     7801 ggttttggac agcccttatt caagaaatcg attgggaagg accaaacaga gtgggtatta
     7861 gcagcgcttc cattaggcgg gtatgtgaaa atgctggatg agcgagaagg cgaagtgtta
     7921 ccgcatgaat tgccacgagc atttaatcag cagtctgttt ataaacgatt tgcaatcgta
     7981 gcggcaggtc ctattgctaa tttcttgcta gcaattttac tttattggat cctttttatg
     8041 ctaggtgtaa atggtgtaaa accagtgata ggtccaataa agcaagcaac acctgctgct
     8101 tttgctgctt ttgaagaagg cgagatcatt caaaaaattg agggtgaatc agtatcaact
     8161 tggcaagatg cacgatgggc gttactaact caagctgttg atcgtaaccc ggttgtagta
     8221 gtagaaacag taaactccca tgggcaaacc gcattaagaa aattggactt cagtcaaatt
     8281 catacggatg atttagatgc taattttctt gaaggcattg gttttattgc ccatcagcct
     8341 agtatggatc ccattatcgg tcaagtcatt gctggtggtg ttgggcatca agcgggatta
     8401 atggaaggag atgaaattct tgccattaac cagaaagaga tactgaagtg ggaggagttg
     8461 gtgagagaga ttcgtaccaa tcctgggcag ttaattgaac tggaagtttt aagaaaaggg
     8521 caaattatcg atgtgatgat tacgcctgaa tcggtaaacg agaacggtca gaaagttgga
     8581 aaagtaggga tcgcaccaaa agttgatccg agtatggttg agaaatatct aatcaacgtg
     8641 agttattctc ccattgtggc attctataaa gcctgctgga agacttggga aatgacggcg
     8701 tttacttttc gtatgttggg taagatgttg attggagaag tttcatggaa gaatgtgagt
     8761 ggcccaatta cgatcgccga ttatgcaggc caatcagcgc agatgggcgt tgcttctttt
     8821 cttggttttc tagctttgat cagcgtgagt ttagcagttt tgaatttgtt acccatacca
     8881 gtactcgatg gtgggcactt gatgtactat ctcattgaaa taattcgtgg cgctcccctt
     8941 tcagagaaga ccatggcact gggccaacag attgggatgg ctttactttt tacactgatg
     9001 atttttgcgg tttataacga tatctatcgc ttagtagcta gttaagaatg aagtttagct
     9061 ttttcttgat ttttctaggt ttattttacg catctacact gagtgcattt gaatctttta
     9121 tcgttagaga tattcgtgtc gagggaatac aacgtacaga agcaggtacg gtatttagtt
     9181 atttaccagt caaagtaggt gaagtacttg atgaaacaaa agcgacttcc gcaattaaaa
     9241 cactctacac aacaggtttt tttaaggatg taaagctcaa agcggataat ggtgtattga
     9301 tcgtccaatt acaagaacgt ccagctattg ctgaaattac gatcaatggc tccaaggaat
     9361 tcgagaaaga taaattaaaa gaaggcctga aacaagctgg attggctgaa tcgcgaatat
     9421 ttagtcgatc actattagat aaggcagagc aagagttaaa aagacaatat atcagccgtg
     9481 gcaaatacgc ggttaagatt actacgacca cgactccact ggaacgcaat cgaatcggaa
     9541 ttaattttga tgtggaggaa gggagaacaa cacgcattaa aaagatcaat attgttggta
     9601 atgaagtttt caaagacagt gatctgatta atcagctaac cctaacgaca cctggattga
     9661 taacttggtt tacaaaaaat gaccaatatt ctaaacagaa gttgtcagcg gatcttgaag
     9721 tattgcgatc atattatctt gatcggggtt atcttgaatt cagcattgac tctacgcagg
     9781 tttcgatcac ccccgatttt aaagatatct atattacgat aaatgttaca gaggggcctc
     9841 agtatacggt atcggacatc aagatggcgg gtaacttctt ggtgccagaa gaagagcttc
     9901 gtaaattaat taaattagaa gctggcggtg tttttgttag agagaggcta actgaatcga
     9961 ttaagctaat tactgatcgc ctcggagatg aaggatatgc gtttgcgaat gtcaatgcgt
    10021 cacccgagat agataaagag aaaagggaaa ccactttcac tttttatatt gatccggggc
    10081 ggcgggtcta tgtgaatcgg atcaatatta ctggaaatga tcgaacgcgt gatgaagtca
    10141 ttcggcggga gtttcgtcaa atggaaggcg catggcattc aacttcgcaa atcaatcgtt
    10201 ccaagcaacg tacggatcga cttgatttct ttacagaagt caatgtagaa acaccgcccg
    10261 ttgcaaataa acctgatcag gttgatatca atatcaatgt caaagagaag cccactggag
    10321 caatcatgtt tggcgccggc tactcggacc tggagggtat tattctgaac ggttctgttg
    10381 cacagaataa tatctttgga accggtaatt ttctcagcct ttccgtcaat acgggacgta
    10441 tcaataaagt cattgccgct tcgtggacta acccttattg gacgattaat ggtgtgagtc
    10501 tcggggttga tgtttttagg cgtgatatca atacagcagc gctaacgaat gtcggtgttt
    10561 ttaataccga tacggcgggt gcaacggtgc ggtttggtat tcctatcgca gaaaacgata
    10621 tcgtatctct cggtgttggc ggtgaatata ctcatattga cctaaaaaat aatagtccac
    10681 agcgttataa agattttgta gaaggaaccc aagacagatt tgatgataaa ggaaatttaa
    10741 tcaaaggaac aaaaggattt gggagttccg ttacaaattt accactaact cttcaatggg
    10801 cacgtgacag acgcgatagc gcaatttgga cgacgtcggg tacaacgcat cggttgtttg
    10861 gtgagtttag cgcgccattt gctgagatga actattacaa agtcagctat gagcaaaaat
    10921 ggtttttccc aatttcgaga ttgttcactg tgatggttga tggtgagatt ggatttgggg
    10981 aaggctatgc aggggatccc tttccatttt tcaagaactt ctttgcgggt gggtttaact
    11041 cggttcgtgg ctataacatc aatacgttag gccctcaaga gcagttgctt aatttacagg
    11101 gagaaccaat agcaaatcgt tttgctgtag ttggtgcaag taagcgcgca gtcgggacca
    11161 tagaattact gtttccagct ccttttatgc gagatgagaa aacagtacgc ttagcggctt
    11221 tttttgatgg gggtaacgtg gcgaatagtt ggtcagactt cttggatttt aataatgata
    11281 ctgggccgtt tgcgaatatt tcatatttta gattttcagc aggactagct gtaacttgga
    11341 tatcgccgat gggaccgctc agagtgagca ttgctcaacc gcttaatcca agagatcgcg
    11401 atccattcca tcgcggtgat gacatacaaa gattccaatt tcagatggga caacagttct
    11461 gaggtacgat tgagcgagcg agccagcaat tatattcata ggatttgtta cagtaagcat
    11521 tatcttttgt ttgctgcatg ctttcttttg ttcttgtcat gctcgattac cctagctaaa
    11581 gaaatgaaaa tcggcgtagt aaatactgag aagatcctaa gagaatctgc tcccgcagtc
    11641 agagcacaga aaaaaattga gaaggagttt ctaacacgtg atgagcagct taagaaagtt
    11701 gctgctcaag tgcgagaata ccaagatcaa ttggaacgaa ataacaaagg tattacagac
    11761 gaagaaagaa gagttaagga acgtgaattg gcaagcctga cacgtcaata tcagcgtaca
    11821 caacagcaaa tgagagaaga tctgaacgcg cgtcaaaacg aagaatatgg cttgatctta
    11881 gaacgagtca ataacgcaat aaagatcatc gctgagaaag aaggctatga tttgattctg
    11941 cagttacaag attcggttta ccgtagtcag cgcattgata ttaccaatca agtaattgaa
    12001 gtgcttatcg agcaagacgc tacacttccg actcaatcag caaaatagcc tgcttaccca
    12061 aatggacaaa ggaaaaataa tatgagtact gtgatggaca tccatgaaat tctgaaatat
    12121 ttaccgcatc gctatccttt gatattagtg gatcgagtga ttgatctcga acccaatgta
    12181 aggatcctgg cgtttaagaa tgtatccatt aatgaaccct actttgtcgg tcattttccc
    12241 catcatcccg tgatgccggg agtattaatc gttgaagcat tagcacaggc tgctgcactg
    12301 ctaaccctga aatccgtgaa agcggagaat gacaagaatc aagtttatta ttttgttggt
    12361 attgatggtg ttcggtttaa aaaaccggtg atggcgggtg atcagttaat gctaaccgta
    12421 gagataacaa ggcaaattaa aggagtttgg aagtatcagg cgcacgctga tgtggatggg
    12481 caacgtgtga cagatgcaga attaatgtgt acggctaggg cgattgatta gtagttataa
    12541 aatcgcagat gaattaaatg atgtacgaag cggcctgggt ctgtggtgtt gatgaagctg
    12601 gaagaggtcc actagcgggg ccagtctacg cggcgagtgt tattttagga gctgattgct
    12661 caattcaagg tctaaaagac tctaaattac tcagtgaaaa aaaatgccaa tcactcacct
    12721 tactgatcaa ggaacatgct aaagcgtggt caattgcctc tgcatcggtt gctgagatcg
    12781 atcaacttaa tattttacaa gcgagcttgc tcgcgatgaa acgagcgatt gaggcattac
    12841 ctataatgcc aaagattgct cttgtcgatg gaaaacacgc gcctcggtta aattgtgaaa
    12901 taagaacgat tgtaaatgga gatcgcctaa ttcctgagat ttcagcagca tcaatcttag
    12961 caaagactgc acgtgatgct gagatgatag cgcttcatga gcgttttcca gtatatggat
    13021 ttaaccagca taagggctat cctactaaag ctcactttga ggcaattcag cggcatggca
    13081 tctgtgaaat tcatcgcaga agtttttctc cctgtcgatg tttcaattaa gctttgattt
    13141 gtagggtgaa attaagccat gattaagaag aagcggattc atataaatga atgatggcta
    13201 tttgttgcta tattgagcct aatcatttga acgatttatt taccaaccct gatgaggaaa
    13261 atatgcttat cgataaagat accgtggtat cgctgcattt ttttaaattg actaaccagg
    13321 aaggggaagt tattgatgaa tcaagtacac cagtgagcta tctgcatggt ggttatggga
    13381 gtatgtttcc atccgtggag ttggccttac aaggcaaaga agcgggctat gatttgtcgt
    13441 tattaatgga gcctgaagat acatttggag aatacgatag cgagttgatt cgtgtagaac
    13501 ctaggaatgc ttttcctgat aatgtgcaga aaggaatgca gttcgaagga agcgcaaata
    13561 attctgaaga tcaaatggtt tatacggtga tcgatgtaac ggatgatacc gttgtcgttg
    13621 acggtaacca tccgctagct ggcatcgcct taaaatttga gtgttcggtc atggatgtaa
    13681 gagcagcaac actggaagaa atatcccatg gccatgttca tggtcctcat ggacaccatc
    13741 attaatcann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt ataacttgaa
    13861 aagattgccc aggctctttg ctgaacatcg aatgactacg caaatataga ggacagtgtg
    13921 cttcctctac cggaaatagc cggtttagtg ggactgatta cacgtttatt tgttgggaaa
    13981 cggctaaaaa gattcaactc aaccacaaat taactcagaa tttctctgaa atacgaaatt
    14041 tgcgtcattt tgcaaagatc tcaccgaaat gggtatttcg gcctaatcga agaatactgg
    14101 ggaaaaaatt cctacgttcc ccagtttgct aaagctaaca aataaaagct tatcgattaa
    14161 atcaacacta tatcactatg cgtcaaactt ggatgtgcgg ttgttccaag caaggcaatt
    14221 tgtatagctg gattaccccc taaaccatcc tgatcgtaat atagaccgcc actggttgta
    14281 ttataaatca acctatcatc agcatcatgc gctgtaactg cgtttgctgc tgaccaaaat
    14341 tgttcacttg ctaaattacc tggattagtt cccaaaccag taaatacgtt aagactaaag
    14401 tgaagtacat ctgagccact ctgaaaatcg gtaatcgtat ctttattact cgtgctattg
    14461 ggttgggagc tgaatacaaa cttatcggcc cctgctccac cagtaagaat atcgttacca
    14521 ttattaccat cgaaaacatc agcaaatgca gtaccaataa gcgtatttgc cccattattt
    14581 cctataatgg tgaggctatt gccaattgcc gctgcatcaa ttttgatagc tgcggtaccg
    14641 gtggtaatag cagtatcagc taaccctaca ccaatgacca ctttctcaat ccctgtatca
    14701 ccagcgaaca aagttagtgt acttgcagct gttgcagcaa aacgaatttc atccacgccg
    14761 gacactccag agtctgcaat ttcagcagta gtatgttcag cagaagtgga aataatataa
    14821 atatcagcgc cgttaccccc attcatgcta tcaatccctg atctaccatc aagtatgtca
    14881 gcataggctg ttccagaaag agtattaggg ccattgttgc caattaatct caaggaattt
    14941 gtaactgctg atgcattgat attgagtgca acggtgccgg tagtgatagc gctagttttt
    15001 gtacctgtac cgataacgac ctgatcaata ccggtatcac cagcaaatag tgtgagtgta
    15061 ctggcaatcg tagcagcaaa acgtaactca tctgtgccca cgttcccggt atcagctatt
    15121 tctgcttgtg catgatgagt tgctgtgctg acgatataaa tatcagaacc atttccacca
    15181 ttcaactgat catgataggc cgttccggta attaaattat ccccggcatt tccaataata
    15241 atcaatgcat tggatgaggc atttgcatta acatttagtg atgtcattgc ggttgtgtta
    15301 gctacactct cgatcccggt accgattaca acccgctcta ttcctgtatc acctgcataa
    15361 agtgtcaatg ttgtaccagc cacgatcgag gcaaaacgta tttcgtctat cccagatgta
    15421 cccgtatcag caacttcagc taatttatgt tcggcatctg tactgattag ataaatatca
    15481 gccccattac gaccatctag tatgtctgcc ccagcttttc catcgataac atcattaaaa
    15541 gccgtcccag taattgagtt gttccctgca tttgccatga tcgtaagcgc atttgcagca
    15601 ctgctcgcat taacatgaag tgcagcagta cctgagctta tcgcatttga ggcggttcca
    15661 gttccaatca caacgcgctc tacaccgatg tctcccgaga aaacagtcaa agtacttgct
    15721 atcgaagcac tgaatcgaat ttcatcgacc ccattcgaac cgttatcctc aatctcagca
    15781 acaccgtgat caattgcagc agaaataaga taaatatccg agccttcacc tccttttaga
    15841 acatcgttgc cacttccccc attcagcgta tcattacccg atgttgctgt tagtatattg
    15901 ttcccagcat ttccagtaat cattaccggg gccaagagcg cgctagcatt gacgtttagc
    15961 gctgtggtcc ctgttgtaac tgctgtagca gcagttccgg tcccaatcac aatctgttct
    16021 atgccggtat cacccgaaaa taaagttagc gttccagtag tggtcgccgt aaatcgaatt
    16081 tcatcgacac ccacattccc actatcattg atttctgctg aagaatgatc agtcgaactt
    16141 tccataatat agatatccga cccttcatga ccgagcaagg tatcaacgcc taacccaccg
    16201 aaaattacat cattaaaagc actgcctttg agtgaattag atcctgcatt gccgatgagg
    16261 ctcaatcctt tcacagcaag gctcgcatca ataccagccg atatagttcc tgaggtatca
    16321 gccgtgctat tcatacctgt tccaattaca acctgttcaa ccccaacatc cccagcataa
    16381 agggttagaa tattaccgct cgtagtagca aatcgtatct catcgatacc actcgtgcca
    16441 ctatcactaa tttctgcctg actatgctct gatgctgagt ttatcaagta gatgtcggaa
    16501 ccactcccac catttaaact gtcaacaaaa ttagtgccta ctaaaacatt tgttgctgca
    16561 tttcctagga tagtgaggcc attaccaaca gtggctgcat tgatatttag tgcggctgtt
    16621 cctgttagcg ttacccgttc aatacctgta tcgcctgaat ataaagttag cgtgccaggg
    16681 acaagcgctg aaaaacgcac ctcatcgtta ccagtagttc cactatcagc aatttcagca
    16741 gcgctgtgat gaattgctga gttgattata taaatatccg ctccatccat accatccagc
    16801 tgatcaacgt agctagttcc tgtaatggta tttatacctg catttcctat gatttggagt
    16861 gagtttgtaa ctgtacttga attgacgttg agcactgtcg tacctgatgt tacagcattt
    16921 gccgcaactc cagttcccat aaccactttc tctattccga catctcccgc ataaagtgtc
    16981 aaagtaccgc tcgttgttgc agtaaaacgc acctcatcga tacccgtcgt tccataatca
    17041 aaaacttctg ctgctttatg gtctgttgcg agtgagaaaa gatagatatc cgttccttct
    17101 ccaccattaa gcgtatcgga tccagcgcct ccttcaagaa tatcattgcc tgctaacccg
    17161 tttaacttgt cattccctgc caatccatag agaatgtcat tacccgcacc accgttcatg
    17221 atgttattgg cagtcgttcc tgtataagtc ccacctgcga cagtcacacc ggtaggcggt
    17281 gtaacgccag ccattaacgt tccgatatct aatcgtcccc ccgtaactgt tgtcgtagct
    17341 aaagagcttg tcggtgtaac agagcttagc agtgcattca gaatttgctg aggtgtcgct
    17401 gatggcagtg cagatgcata cagcgcgact gcccctgcca cgtgaggtgc agccatagag
    17461 gttccttgta acgccgcata gctattgcca ggaaccgtag aatagatgct ttgacctgga
    17521 gcacctagat ctactgttac gttgccatag tttgagaaag atgcgagcgc acccgaactt
    17581 gttaatgctg ctaccgaaac aaccgcatca aatcctgctg ctgcggttgt attgaaattt
    17641 gatgggtaat ttgcagatgt gttcgtattc agactactat tgccagccgc agcaataaag
    17701 aggttaccgg ctaatgctgt tcttactact gcatcatagt tactctggga ataaccactt
    17761 cccccccaag aattgttagt gcctacaaaa ttctggcctg ttcctaccgc taggctggca
    17821 ttggtataat aatcaatcgc agcagttgtc gttaaagatg ttccgccaga aggtcccagt
    17881 gctttgaggg gtatcatctg aatattccaa ttcacaccca ctacacccgt tccattaccg
    17941 cccattgcgc cgatcgtacc tgccacatga gtgccatgct gcgcatcgtc catcggatta
    18001 ttatcattat taacaaaatc ccaaccaacg atatcatcga tgtagccatt tgaatcaaga
    18061 tcgttaccat cttcccaacg actgtcagct agcaaatctc ctgcatcaat ccgactgtta
    18121 ttattcaaat cactgacata agtaacattc gctgcatgat tcaaatcaac aaagcttata
    18181 actccatcgc tatccacatc ggtaagagat ccacgtagcg tggtactaat ctctttttga
    18241 ttaatccaaa tattttgaaa aagatctgga tgggtataat caataccggt atcaattacg
    18301 ccaagaactg tctttgtggt accaatgaat ccagtattcc aagcttcact tgcttggctg
    18361 ccatatatat tagcaggggt tgagagatcg ccatacattc cccagaggtt accattggtg
    18421 tagtagctat catcactggt tgattcaacc ttaacaactg aattttgctc tacaaatcta
    18481 acatttgaat gattgcttag tgaatttaaa gtgaaatcaa gtgaggcggg atctttaact
    18541 ttggccaata ctaaatttgg attagttggc atgattggtt ttataaactc ggcgttgttc
    18601 tgttcaagta ataaaacttg ctctgctaat gttatttctg gaaagaattg aattaaaaac
    18661 tctccagtcg tcatttcatt ttcttcacta aacatagttt ccgtacacct ctcgatagaa
    18721 ggatttcatt ttgaaaatac ttcagttatg taatgcttgt ttctagatag gtatgccgcc
    18781 aaaattagac tgttggtgac tatcacaatt gtgattgttg tgattgcttg aaaaaaaggg
    18841 gaaaaatatg taacggtata aaaatcgata ccttgataac cgctgcaaaa tgagaagtta
    18901 ccgttgatag gtataagcat gaatgaggtg taatcaagac ataaagcaat ttaaatcgca
    18961 gtgcttactt gaattgcttg taaaacgatt aaacccaaga ttcttactga gcgtatctta
    19021 gatttttttg tagcgcatga aacattacta atgcgcacct caaagtttag taaaatctga
    19081 ctatctataa agtcgccagg catgatcgca atgattgcat ttgccccagc ttgataccta
    19141 acgattcaat tttatggaat gaattttatg aatggcattc aaaaatactt cttagtatta
    19201 acgagtctaa tgcttgttag taacctaagt tttgcttcca cctccaattg cacacaatac
    19261 tcaacttctg caatttacat tgcctcaacc ggtctcgtct gtttacagca agtaaaggtc
    19321 attgatcaaa gtagtattca actttatgaa gcttccctgc aatggcttgg ttctgataat
    19381 cccaaccagt ttagcttggt ccacgcagat ttagatacta cgcctgagca gctgagcata
    19441 ccttcttttt cagctgagaa tggcatgctg gcaataccga aggtagatat tcctaggcaa
    19501 tttggtactg aacgctattc cgtcaatttg acgctggctc ccaatagcga ttcttatcta
    19561 tttaatcttg actcgatatc ggtttatatc aatcctgacc atgtgccaag agttacttgg
    19621 aaaccttatg gcatgcttaa ttccaatgaa cggcgtgctg tcgatgcgtt gggtatgtcg
    19681 cttccttacg cgcgactctc cgatgcaatt tatgattttg ataatactgc ggttgatcag
    19741 tgggacttga tcgacaaaat tagccgagat tcaggcatgc aagctggcgt ttatcaacat
    19801 cgtgaaacct tcgaacttgc tctcgccttt cggggcacag aaacctgtga tttcccttgt
    19861 agcttggatg aaacaaaaga atatttgcgt gatttagcga ctgatactgc gctgactttc
    19921 ggatttgatg atgctcagtt caaacacgcc ttaaattatg gcaaagatat tgtgaatcgc
    19981 tttcccaatc gtagtattac gctaacaggg cactctctcg gtggtggctt agcacaagcc
    20041 gttggtgctg ttttaggttt aaaaacattt gcatttaatt ctgcgccagt tccggatgat
    20101 ttttttgatg ataatccggt gtcgttacca gaatcagccc tcaatgaatt aattcacgtt
    20161 ctcgctgata tccatgaccc agtttccaac accgatgaaa ccggcaagtt ttatattaat
    20221 tcaaagcatg cagcgccttt aattcagttt gatttcgact tgaaagaaat cacgcctaat
    20281 cgtctcgcag aattagataa attgagattt aatcgacatg gaatcactaa attctttgaa
    20341 aacgcgagta gtttgatgac aatttacgct aacggatggt aaagattagc aatcgtcaaa
    20401 gattaatact tcacaaactc aatggatata gaaagtaaac atacacccat gatgcagcag
    20461 tatcttcgca tcaaagctca acatggagac aaattattgt tctaccgtat gggtgatttt
    20521 tacgaattat tttacgaaga tgcagagaaa gcagctaagc ttcttgatat tacattgacc
    20581 cagcggggca tgtctgctgg cgaagcagtc aaaatggctg gtgttcctca ccatgctgca
    20641 gagcactatc ttgccaaact cgttaagctt ggagaatcaa tcgctatttg tgagcaagtt
    20701 ggtgatcctg caaccagcaa aggccctgtg gcacgcgaag taactcgaat tattacgccc
    20761 ggcacgctaa ccgatgcagc actactcgat ggaaaatccg atagcattct agtggcgttg
    20821 acatgtcaca agtctgtgct aggccttgca tggcttaatc tcgcggcggg tcaattctgg
    20881 gtgcttgaaa caacgctaga caatttgctc agtgaaatag aacgtatccg tcctgctgaa
    20941 ataatcatcc cagagtcatt cgatattcca actgaactca aagaatatgg cgtaatcaga
    21001 cagcttccgc ccaatcaatt tgattttgat aatgcagtac gaaatttaac gacactattt
    21061 agaacgcatg atctttcggg atttggctgt gatcagttgc atgcggcact tcaagcagcc
    21121 ggtgcgcttt atcactacct ataccttacc caaggtacta atctcactca tattgctggg
    21181 ttgaaaatag aacatgatag cgcctatatc cgtatggatg ctgttacacg taaaaatctt
    21241 gaaatctcag aaacattgag cggagaaacc tctcctacct taatgtcact attcgatact
    21301 tgcgccacca atatgggaag ccgcttactc aagcactggc ttcatcatcc actcagaaac
    21361 gatgcaatga ttcaaaatcg cctggaaagc gtagcgttcc taaaaaataa tctaaaaggt
    21421 gcgccacact ttactagaat caagcaattg cttagaccga tagcagatat cgaacgcatt
    21481 accacgcgca ttgccctcaa atctgcaaga ccgcgcgatt tatctagcct gcgcgatagt
    21541 ctcattgcac ttcctgaaat ggctgaaacc atcgccaatc acctcaacgc ccatctcaat
    21601 caattagcga atgctttaat accggatgca aatctggtca agttattacg ccaatcattg
    21661 caatcggaac ctgcgctatt attaagagaa gggggagtca tcgccgatgg atttgatgaa
    21721 gagctcgatg agttgaggat gctgcatagc aattgtggag aattcttact acaactagaa
    21781 acgagagaaa aaacacgaac gggaattccc acgctcaaag ttgaatacaa tcgagtgcac
    21841 ggattctaca ttgaggtcac tcatgtccat acggataaaa tcccggctga ttatcgccgt
    21901 cggcaaaccc tcaagaacac tgaacgctac atcactcctg aattacaggc attcgaaaat
    21961 aaagccctgg cagccaagga taaggcgctt gccaaagaga aacaactata tgaagcatta
    22021 ctggagcagt tgactccgtt cattactctt atgcagcaaa tcgctcgaag cattgctgag
    22081 atcgatgttt taagtacctt tgccgagcgg gctgaaacat ttaattatgt catgcccacc
    22141 ctgacagaag aagcaagcat tacgattcaa gacgggcgcc accctgtggt agagacccaa
    22201 gttgatcaat atatcgcgaa tcacttaaat ttaggcgcat cgagcaacga taagaaaatg
    22261 ctaatcatta cgggccctaa tatggggggt aaatcaacct atatgcgcca aactgcactc
    22321 atcgtcctgc tagcgcattg cggtagtttt gtgccagcaa attgtgcgcg tatcggtcca
    22381 attgatcaga tctttactcg aatcggcgct gctgacgatt tagcaggagg gcgatcgaca
    22441 ttcatggtcg aaatgaccga gatggccaat attctcaatt atgcaacccc gcagagcctt
    22501 gtattagtcg atgagatcgg ccgcggcacc tctacatttg atggcttagc gcttgctttt
    22561 gcgattgctc gatacttatt gaacaagagc caaagctata cgctatttgc cacccattat
    22621 tttgaactaa cgaatcttgt taacgaatca gaacgaatcg caaatgttca tttaggcgtc
    22681 gtcgaatatc aaaatcggat tgttttttta cacaccctca atgaggggcc tgcaagccaa
    22741 agctatggca tccatgttgc agagctggca ggtgttccca aaccgatcat aagggaagcc
    22801 aagaaaattc tggcccattt agaacgcgaa gcaaatcacc aaaacccaca acccgaccta
    22861 ttttcagcga tcagccagga tgaaacgatt gattcactcc ccctctctca ccctgtgatg
    22921 agtcatttaa atactttaaa acccgatgaa ctcagcccca gggaagcatt agtggaactc
    22981 tataagctaa aatcattact cgatgaacaa tgatggtgag caggattacc cataattgct
    23041 gataaaaaac caatccagca atctagtttc ttaaagttac cttctctatt tgtggcatta
    23101 aatagtctcg ttataatgat agccaacttg ggaagttatc tgaaaaaata aggagatatc
    23161 atgaacgatt ccgtattaga tttcatttgt gacgtaatag attgcgtcat ttcgaacgtc
    23221 taattctagt tatgtttctt agcgagtggc attgctgaac aatggcagta cgtatcactc
    23281 ctcgtctcgt tgagctgact tacgaggctg ctctaaaggc tttctggcga aaggaggcac
    23341 tccgcaagtt cttgcgggcg tctcacgtcg cggagacgtt tcttgccact tgggcgccgg
    23401 aggaaagcaa gcgcgacttc ctcgacaaga cgtttcagga gcttcagcga acggaccgag
    23461 gaaaggcagt tatcttcgaa atggctcgct atctctccga gcagacgact tttccggacc
    23521 tccgcaactg ggaagactct gcgcagaaga tcacggacgc cacaaaggca gtcgcggagt
    23581 taaaggcttg gctaaagacg caagacgaag agattcgaag cgaaaaggaa cgagaggaag
    23641 ccaagaccaa ggcgagagat gagcagcaga agattcagcg aagcctcacc gacaaggcga
    23701 agctacaaca acgtctcgac gcgcttcatc ccgccgtggg cacccaacaa ggtggttacg
    23761 attttcagaa ttggttctat gacctcttgg acttctgtga gatccagaat cgccggccgt
    23821 atgtcagtgc cggcaggcag atcgacggtt cgctgacatt cgacggtact acctacttgg
    23881 ttgagttgaa gtttacggga agccaagctg acgctacaga catcgattcg ttacgttcca
    23941 aggtggacga caaggctgac aacaccatgg ggataatggt ctccatatcc gggtactcca
    24001 gcgtcgcact gcgtcaagca tccggacgaa agacaacgct cctgctcgtc gatgccatgc
    24061 atctctatct ctttctgtcg ggcacgccgt ccttcggaga catcatctct aggctgcggc
    24121 gccatgcctc gcaaactgga gaagcttatc tagcggtggg caaatttggt gggtaggagg
    24181 cataacctcc gccagcaggt tatgcgggcc gttaggtttt gtaattccat catataagag
    24241 taaggggtca ggtcttgtag gttcacaaaa atggcacgga atataaagga actgttgatc
    24301 aagcttcgtc gctttttgca aaacaaacat cagttagaaa tttacacgtg ttcgacgtcc
    24361 tcaaacgaat agcgaggctg aacgcggcat taaaactgtc ttggaggtgt ggcatgcaca
    24421 aaccgtttta ccttatgaaa agatcttgag ttcagccttg tcagattcgt caacttctat
    24481 aatactgtga aacctcatgc aggaattagc agcatcactc ggcatgaaaa attaacaaac
    24541 tttcacttcg gattgtaacc aaccctaacg attctcacgg cttaagtgtt tatgtagccg
    24601 tctaaaatta ggaagtggga tcgtttaatg gactgataaa ggttcaaatt gtaatgatac
    24661 ctgaccgaaa gcaaagtact ttagttctgg tttgtaagcg acctaaaatg ggagtaagca
    24721 agcaacgatt agccgttaaa ttgggtcgtg agaaaacgtt ggtgattgca aaagcgctgc
    24781 ttgattgtgc tttggaggat gccgcagagt ggcaagggcc tattgtcata gcgcctgctg
    24841 atgatgccga ttctgagtgg gctgtttcat tactacctga agcaaggcag atcgaggttt
    24901 ttcctcagag actaggtaat ctaggacaac gattaaatgc gcttgaccaa tcgttgcgct
    24961 gtgtcgggca tactcagcta gtatacatcg gtagtgatgc gcctgtgctg caatcggtgg
    25021 attacattgc ttgccaacag gcattgcaac attacgatac cgtgttgatt cctgcaagtg
    25081 atggaggagt agcgcttatg gcgagtaatc ttcattggcc agagttagca gacttaccct
    25141 ggagcaccag tcaattagga aaagcccttg tcgtgcgttg cgagagtgct gggcaatctg
    25201 ttgcattgct cgaacaacgt gctgacgtgg atgagtttgc agattgtgta aaattaatat
    25261 caaatttgaa cgatgatccg cgacccgcaa gacaaacttt actaaaacgc atcaaaagca
    25321 ttattgaccc ttaaaacatc atgccaagcc taagtattat tattccttgt tatcgtgatc
    25381 acgctcaagt cgcagtgttg cttcaacagc tcagaatgtt atcggcttta cccaatgaaa
    25441 taattgtagt agatgcagcg ggtgattcgg catgtcaggc gatctgtatg cagtatgatg
    25501 cgcaatggca tcacagtgag ccttgtcggg ggaagcagtt attgcaaggt gctcaagttg
    25561 cgacgggtga tattttgtgg tttcttcatg ccgatactcg cgtttcaccg aattcgattt
    25621 caagcattat gcaggcggtt gagcgtggcg caattggggg ttattttcgc tttgtattcg
    25681 atggaccccc tgcttggcct atgttgattc tggaacctgc cattgcactc agaagtcgtt
    25741 tcggaacgcc ttatggtgat caaggaattt ttgtcagaag aatcgcttat ttcaaagtgg
    25801 gaggtcatgc accgtggcca cttttcgaag aagttccttt agtgaaggga ttacgcaact
    25861 taggacattt tcaaccttta ttggattcaa tctgggtaga ttcgcgacgt tggcagcgcg
    25921 atggctggtg gcatcgcaca ttgcggaacc gcatgttagc attgaaattt atattgggcg
    25981 catcgcctga tacattagcg aaatcctacc attctcaatc gtcttaaagg agaaagctat
    26041 gagagaggtt gttgaagcgt attatggcga aacgttaacg tgttcagctg atttgcagac
    26101 taatgcctgc tgtaccgatg agggtatgcc gagttattta aagccattaa tggccaagat
    26161 tcatcctgaa attgcaaatc gttactatgg ttgcggtctt atagcgccac cgctgttaac
    26221 tgggttaacg gtgcttgatc tgggttgtgg tgctgggcgt gatgcttatc tcttgtcaca
    26281 aatggttggt gaacaaggcc aggtcatcgg ggttgatatg actgaggcgc aattagcggc
    26341 tgctacccaa taccaatcgt atcatcgcga tgtttttggt catgcagcga gtaatgtacg
    26401 attcttacag ggtgatatcg agcaccttga tgagcttaat ctagccgatg gcagtgttga
    26461 tatcatcgtc tcgaattgtg tgatcaacct tgcccttgat aaatcggctg ttttaaagga
    26521 agcctttcga gtattgaagc caggtgggga gctctatttc tcagatgttt atagcgatcg
    26581 gcgtatacct gcagtactag caagcgatcc gattttgtat ggagaatgct tgggtggggc
    26641 actgtactgg aatgactttt taccgcttgc gaaacagtgt ggatttatcg atccacggct
    26701 ggtgagtgat cgaccaatca cgattggaaa tactttactt gcggataaag tagggaatat
    26761 tcgattttat tcggcaacct atcgcttgtt taagttaaat gatcttgagc cggcttgtga
    26821 agatcacggt caatcggttg tatatcgggg atcgattccc gatcacccgc atcattttac
    26881 gctggataaa caccatcgga tcgagactgg gcgtcagttt cctgtttgtg gcaacacctt
    26941 tcgtatgttg cacgataccc ggtttagc
//
LOCUS       DAOQKQ010000010        24854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_827_length_24854_cov_5.401427, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000010 DAOQKQ010000000
VERSION     DAOQKQ010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 24854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24854
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_827_length_24854_cov_5.401427"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..302)
                     /locus_tag="PKZ49_01265"
     CDS             complement(15..302)
                     /locus_tag="PKZ49_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35220.1"
                     /translation="MHRRHMTPGQQAAIVASAQDWAKAQVQGANRYTIKTGNVAGLET
                     VADRASESGASERTQRMADKVAKADPELAKQVAHGEISLPKAVEKISPKKK"
     gene            complement(425..709)
                     /locus_tag="PKZ49_01270"
     CDS             complement(425..709)
                     /locus_tag="PKZ49_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35221.1"
                     /translation="MPTYTPRGINAGIEINESSGLDKEISLIPIMSGYRDKDTFEVSL
                     TTYYEAIDTDLDIVIRQDKILTASWFDFDVYKKLNHKKRQYNTSYIYVND"
     gene            complement(758..1204)
                     /locus_tag="PKZ49_01275"
     CDS             complement(758..1204)
                     /locus_tag="PKZ49_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein"
                     /protein_id="HND35222.1"
                     /translation="MKWITRERPKIDRIACPWLIARFIDKESAFLFVPPDQVVAEAKL
                     TGAIPFDIPGVELSHVGNECSFDAFLKKYNLTSPALQDLAAIVRGADTDHLDLAPQCA
                     GLLAISLGLSHNFKDDHEMLAHGMVMYDALYAWCCHARGERHNWDY"
     gene            complement(1216..2571)
                     /gene="chrA"
                     /locus_tag="PKZ49_01280"
     CDS             complement(1216..2571)
                     /gene="chrA"
                     /locus_tag="PKZ49_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HND35223.1"
                     /translation="MDVPKQQFPDIADAPKKISLSQAFWYWLKLGFISFGGPAGQIAI
                     MHQDLVERMHWISERRFLHALNYCMMLPGPEAQQLAIYIGWLMHRTWGGIIAGILFIL
                     PSLFILIALSWVYISFSDIALVNGLFYGIKPAVTAIVVQAAYRLGSRTLKNSLLWSIA
                     VASFVAIFVFNLPFPLIVVSAALLGYVGGRIIPDSFKVNSNHSKANKSFGLALIDDHT
                     PTPEHAKFRWLRLVKVVVIGGLLWLLPMILLTISYDWDHTFTQMGWFFTKAALMTFGG
                     AYAVLPYVYQGAVEYYEWLTPTQMIDGLALGEATPGPLIMVVAFVAFIGGYVKALLGP
                     DMLFISGTMAAILVVWFTFLPSFIFILAGGPLVESTHDNIKFTAPLTAITVAVVGVIL
                     NLALFFGYHVMWPQGFSGNFDWISALIALLAGIALFQYKRNVIEVIAICAIIGLILNL
                     T"
     gene            complement(2811..3107)
                     /locus_tag="PKZ49_01285"
     CDS             complement(2811..3107)
                     /locus_tag="PKZ49_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35224.1"
                     /translation="MLSGVKADPSANLQQGETGRTCEELSELSGVSSRNIYKVKNIIS
                     TGSDELIETVRAGEISINVAEDIATLPQEGQKEIDLTDTSLNRVIVRSGRDYYN"
     gene            complement(3218..3418)
                     /locus_tag="PKZ49_01290"
     CDS             complement(3218..3418)
                     /locus_tag="PKZ49_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35225.1"
                     /translation="MFMACHSQEEIAEVAGADQATIKRKVDDFMQNSNLAKMHKTLAN
                     HEDSEFQIPLYNVWTKSKITTA"
     gene            complement(3509..3946)
                     /locus_tag="PKZ49_01295"
     CDS             complement(3509..3946)
                     /locus_tag="PKZ49_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35226.1"
                     /translation="MEKRGRKSSEENYQQGFMLQSCYLRLEPDEALSMAEKVIWEEVI
                     SNVPNNFFTPMHKPLLEMYCKHVAQSRLLNKELHTLNTDLLKTREGLRHYDKLLRMRE
                     RENKMASSFATRLRITKQAVHPTTAFRALANQSTDEKPWLFKC"
     gene            complement(4147..4422)
                     /locus_tag="PKZ49_01300"
     CDS             complement(4147..4422)
                     /locus_tag="PKZ49_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35227.1"
                     /translation="MSILAELIKGKKTYDTIPDEAVTEFNNEVTCSITDNSPSIEAVR
                     AWLYKIEEPEEYHHIVLDKCKADDEALAYFLKHANGEFEVKDLLNKN"
     gene            complement(4542..6119)
                     /locus_tag="PKZ49_01305"
     CDS             complement(4542..6119)
                     /locus_tag="PKZ49_01305"
                     /inference="COORDINATES: protein motif:HMM:NF024547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HND35228.1"
                     /translation="MIENSISPSESFSNNLANVANTGELNLDDAVDPPINSGWPQPQP
                     ITARIQSEPYPIDALPDVIRLAVEEVGAFVKAPTVLVASSALGSLSLACQAHVDAKRA
                     EKLQGPTGLFLLTIADSGERKTTCDSFFTSAIRQYQEEQAEAMKITVKEYESENAAWL
                     AEREGLLSAIKEAGKKSKSTEVFKENLKQLEYSKPEPLRIPRLLYVDTTPEALAYNLA
                     KQWPSGGMISSEAGIVFGSHAMGKDSIMKNLSQLNQLWDGNSLAIDRRTSESFIVKGA
                     RLTVALQIQETTLKSFFNKSGELARGTGFLARFLVAWPESTQGSRMFTEAPQSWPYLS
                     EFNRRITAILNDPVPIDENGALTPTMLELSPQAKQAWVSYHDTIEKQLANGKELYDVR
                     DVASKSADNAVRLAALFQWLNKPNQAISLDAFDSASRIVSWYLSESRRFFGELALPEE
                     LANAAKLDSWLIDYCKREQTHLVAKNAVLQYGPLRKKEKLDAATQELAELDRLQVQKD
                     GKRIILVINPNLLDFNN"
     gene            complement(6120..6971)
                     /locus_tag="PKZ49_01310"
     CDS             complement(6120..6971)
                     /locus_tag="PKZ49_01310"
                     /inference="COORDINATES: protein motif:HMM:NF024755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HND35229.1"
                     /translation="MLNLSEVVKRQFRQAMQAAGIAYSGEIIPDGQLHRFHIEGHKKG
                     SLNGSYRLHIDNRPAGYFQDFKSGISQKWRSSGNSYVSTDVIKSFKEANHQREIEARQ
                     KQEEAAKKAAEIWQKSKPVNNQVDHPYLVKKAVQPYGARLYCNALAISIYNEGNRLVN
                     LQFIDPEGCKRFLSGGRKHGCFYIIGDLSPRILIAEGFATGASLFKSCGQRVVIAFDA
                     GNLLPVAKNIRELSPDSEIIICGDNDLSGIGQAKAREAAIAIGGKVLIPPRPGMDWND
                     YLTMEKK"
     gene            complement(6964..7866)
                     /locus_tag="PKZ49_01315"
     CDS             complement(6964..7866)
                     /locus_tag="PKZ49_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35230.1"
                     /translation="MRTSQFSIYRASTLKRKRRTKDELNQLDEKIIQVLKEDHPQSVR
                     HIFYRMTDVRLNQPVEKSEKGYRHIQDRCVKLRRSGLIPYAWIADMSRQGYFVNTFID
                     ASDFIMSMAGLYRADLWSDADYRCEVWTESRSIASVIRSLCNELAVDLYPCSGFSSLS
                     FAHAAAEQHISSKDNRPLIIYYIGDYDPAGVLIDQSLERELRLHLNGYIEMDLRRIAI
                     NENQIEQYDLPRKPRKKTDIRSKQIESTVEAEAMPAHILRQMLREYIESLLPEGALYA
                     TKIAEQSERNGLIALANRTGGYRA"
     gene            complement(7863..8231)
                     /locus_tag="PKZ49_01320"
     CDS             complement(7863..8231)
                     /locus_tag="PKZ49_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HND35231.1"
                     /translation="MASKRNKESIRHGGVTMAQYENSTIIQRKRILDFLRTRPLDTLT
                     ARKELDIMHPAARVMELRKQGLDIKTIKIDRQSDCGEIHRVACYVLEVRAAATAPTDK
                     EDGILRQETDNLKHNMEVSL"
     gene            complement(8186..8398)
                     /locus_tag="PKZ49_01325"
     CDS             complement(8186..8398)
                     /locus_tag="PKZ49_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HND35232.1"
                     /translation="MSTNLDHFQSIERITRPSLSTEEAAFYLNRKPQTLRCWAMRENG
                     LIRPLRINGRLAWPVKEIKKVLGMEA"
     gene            complement(8577..9317)
                     /locus_tag="PKZ49_01330"
     CDS             complement(8577..9317)
                     /locus_tag="PKZ49_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35233.1"
                     /translation="MPNNSNGRLHFDEITKARLYQLLSSAFKKKNSYNYNPDSEICLM
                     ISCFECFAEAIPESEKFLPEKQQRRQERINSLAAHIEGLINQIEALDSPALGYAIHRG
                     CEEISNIRKIANPFQYSFDAILEAEFLRDKNNIKELKAFSLGLRKAAKDLPVHVGNTS
                     GNNYPWYSQKSETAVALALEKSFFEAGIDFTTSNNGLAAECLRAIYDLGGLKIDRVDY
                     WLGKAKNHRDSLFSISKRCRNIDQGNNS"
     gene            complement(9461..9697)
                     /locus_tag="PKZ49_01335"
     CDS             complement(9461..9697)
                     /locus_tag="PKZ49_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010893244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HND35234.1"
                     /translation="MHNPAHPGEILREWLPEEMTVTQAAKELQISRVTLSKVLNGKAG
                     ITATMALRLSAWLGTTPDVWLGVQTQWDLWLAKQ"
     gene            complement(9724..10005)
                     /locus_tag="PKZ49_01340"
     CDS             complement(9724..10005)
                     /locus_tag="PKZ49_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HND35235.1"
                     /translation="MQIKTKSGRVLVLPSPEEDAQITAAALTDPDNQPLTNEELQQFK
                     KVRGRPQGSGKKEQVTLRLDTDILEQFKASGNGWQTRINDALRDWIKQH"
     gene            complement(9989..10258)
                     /locus_tag="PKZ49_01345"
     CDS             complement(9989..10258)
                     /locus_tag="PKZ49_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HND35236.1"
                     /translation="MKVICDATKDAINKEKHGVSLHDAMHIDWDAALIWQDTRHDYGE
                     VRMIALAPIDERLYCVVYVDREDHRRIISLRKANYREVIHYANQD"
     gene            complement(10312..11601)
                     /locus_tag="PKZ49_01350"
     CDS             complement(10312..11601)
                     /locus_tag="PKZ49_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HND35237.1"
                     /translation="MKRKGFTAKVISEFQCEFGKSQSLFWDADHPGLGLRVAKGGKKS
                     YIFETKLHGRTLRLTIGDVLSWSLNRAEKEATRLKALTDQGIDPRQLEAEKRVAAEVK
                     RKREHAEKINLRDAWEEYINERKSLWSDHHLNDHIKVMHEGGQIRGRSKELTVPGVLS
                     SLAKVRLVDLTHERIEEWAKIESIKRPTRARLALRLLKAFLFWCSRHSSYKEIVIDNA
                     AQSKTARENLGKAKAKNDALQREQLAAWFQAARQIPNPVISAYLQSLLLTGSRREELA
                     NLRWEDCDFQWNSLTIRDKVEGQRVIPLTPYVSQLLSNLPRRNHWVFSSPTAATGRLI
                     EPNKQHSKICSIIGVSVSLHGLRRSFATLSEWVETPAGIAAQIQGHKPSGVREKNYIR
                     RPLDLLRLWHTKIETWILEQAGIEFIPIKAGLHVVNK"
     gene            complement(11891..12622)
                     /locus_tag="PKZ49_01355"
     CDS             complement(11891..12622)
                     /locus_tag="PKZ49_01355"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HND35238.1"
                     /translation="MARLPRFVLVGHPQHIIIRGNNREPVFNAEEDYRFFLDKLADAA
                     KKHQCDIHAYVLMTNHVHLLATPHKAESIAKMMQMLGRYYVQYYNYTYQRTGTLWEGR
                     YKASLIDSEQYALLCYRYIELNPLRADMVKHPSEYPWSSYRSNALGQYDALITPHSMY
                     IALDQDEAQRQARYRDLFNGQIDEQNLASIREATNKAWVLGSDYFKQKIEAQLNRRAD
                     KLTKGGDRKSDEFRQNKKFNRIRPH"
     gene            complement(12724..12987)
                     /locus_tag="PKZ49_01360"
     CDS             complement(12724..12987)
                     /locus_tag="PKZ49_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35239.1"
                     /translation="MKLPISIIEKNGDVTVCASVHEAEMEMEPIDVQRGEYVVTDADG
                     LLLSVGVVTEEVPLFWGLWKGRVKKVRITGLLASAENQKGRAR"
     gene            complement(12987..17330)
                     /locus_tag="PKZ49_01365"
     CDS             complement(12987..17330)
                     /locus_tag="PKZ49_01365"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HND35240.1"
                     /translation="MSFSNYALANHTDTTSPASCPDIEVNGGQSLTYWFKDYYTQKPY
                     QGGQIVPIGTRLLHFIRAEAYGHCEIRQLEIPPSSACYTGINNERGINFVFAGLQFPN
                     GSFGGFYKSIAKSWDECGIPEFGQVLETGITPCTQPSQLLFNQTGRYLVYSRLGTLAT
                     ACLIKPDIFTETTFFDVVGPNKSLKCPHADSRGNPCSTSTGNKHQQEEDYTGPNLPFT
                     RFYNSQFNANVGIGYGWMTAYHKRLVVGEVSGKERIILLRANGASEMFSQQTNGSFAG
                     DSDTSFNLVKNTSEYIVTDRLGNVETYNVQGLIQAETDSAGNTTTYAYNSTGQLTTVT
                     GPFGHQLQFTYDATHHLIETATLPDSSVLHYSYQQPAQDKAYNLTSVTYPDSSSKTYH
                     YEDSSLPNHLTGITDQNGIRFATWTYDAQGRALTSAHAETTNAAPQERFLFSWGSLYE
                     TTVTDPKGNKEVFKFTPYLGVKKLTEKTNLADNKKILQTFDGRGNLTSHIDEEGRKTT
                     YTYNTANQRLSMTEASGTTSARTTNYTYYSNTVDLITSIKKPSVVSGNIATTTIDYNA
                     DLLPERITQAGYKPGGAAVSRITTLNYNSYGQITSIDGPRGDVNDLTTFTYHSCTSGA
                     ECGRLATVTNALNQVTTYQAYDGAGRVTSITDAVGLLTQLTYDTLGRIIEIKQTSPIG
                     IVRITSYSYNTIGKLASVTTPAGTTLNYSYDNAHNLSTVTDNLGNSIKYKYDLSGNLI
                     EEKINDPSGTLARKIQNTYDVRNNLQTINFGGSLTQLVQDAIGNLRSVTDANSHITTY
                     EYNSLNQLTRTIDALSSPVQYSYDKQDNVVSLNTPNGATTASTYDDLGNLLSETSPDR
                     GITTYSYDQAGNQIGQTDARGVITTMTYDALNRILTKGYPGSNEDITYAYDSCLNGIG
                     HLCSITDESGTTTYSYNVFGNIVERIQTIEGVSYTTQFTYNADNRVTQIIYPTGHTIV
                     YSRDALGRISAANLSISGQAQPLISNVSYTADGLLRTQTFGNGLQETRSYNGMRLLEN
                     WQLGSTSTTYTYDAVGNMLTRASSTGTQTFTYDSLDRLLTDAGIGQNYIYSYDANGNR
                     LTHSKNGSQIQYGYDLNSNRMNWYDSAAALLDNAGNTLAQGGLTMTYNQANRLETVGT
                     IGPFTYHANGLRITKPSPNGTTIFHYDLNGQLIAETNDAGGFVRVYFWADEIPMAQFD
                     TQLTYLHVDHLNTPRMGTNDAGSVVWSWHSDGFGNTTPNEDPDGNGQVTTVNLRFPGQ
                     YYDVETGLHYNYFRDYSPQTGRYIQSDPIGLTGGINTYGYVGGNPLSFMDPLGLWAWG
                     DPLPQGLVDACAGFGDGLSLGVTAGIRALMRTNDVVDFSSPEYLSGMAAGVAVTTRGY
                     ATGAELSIGRSFRLAPWGNRTGHPTGRYPHYHRRGGPLDAKGNPPPGQSIRRHRPWDN
                     RSTDKCGCDRF"
     gene            complement(17559..17708)
                     /locus_tag="PKZ49_01370"
     CDS             complement(17559..17708)
                     /locus_tag="PKZ49_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35241.1"
                     /translation="MKLLLCAFIWLIAASIIPEITTVVTFLWVLIVFIVGITNIAVKI
                     LDFLF"
     gene            complement(17846..18235)
                     /locus_tag="PKZ49_01375"
     CDS             complement(17846..18235)
                     /locus_tag="PKZ49_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HND35242.1"
                     /translation="MRYPIALNFEDDDHAISAIVPDLPGCFSAGDTEEEALINTQEAI
                     ELWLETVIDDGGAVPEPKSIKAYKDDPEYKDCVWAIVSIDLAKLSDKIERVNITLPSR
                     VLRRIDAAAKAAGESRSGFIAHAVLKS"
     gene            complement(18308..18442)
                     /locus_tag="PKZ49_01380"
     CDS             complement(18308..18442)
                     /locus_tag="PKZ49_01380"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HND35243.1"
                     /translation="MNSKEIIKLLEKEGWVLRNTKGSHHIYTHPERSGHITVPHPKKD
                     "
     gene            19118..20257
                     /locus_tag="PKZ49_01385"
     CDS             19118..20257
                     /locus_tag="PKZ49_01385"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HND35244.1"
                     /translation="MKTSTFKSMRLISYTLAGLFVSTSAQALFINATVTGGTPILGGT
                     TDLLKSNRTVSDNNISTSVSASKTQATNATISQFDASQGVLVGVTTTLTAGNNTTYLR
                     ADGANAARGSASVDTSWTGSGGLNTSGNLNSVSKSGNTDGADNSWNALSQTISNGANL
                     NSWIGAGNLISTINTTINSNRIDGGSNIALIADISSSGANINGATAPSIIDLSAAYSV
                     TYNYMEHAVASFDGSSTLTSLDIDFGTIVLGDLVNSISFDIFNLAGVNGVALDLTSIS
                     PSGDTATFVTDLGFFSDLIHGAHNSYSASFDPTSAGTFDATYLLTLSDDDATGASASR
                     HTYSLELHLTGTAVNPTAIPEPSMIALLGAGLLSLFGFSRRSSLK"
     gene            20645..20884
                     /gene="hfq"
                     /locus_tag="PKZ49_01390"
     CDS             20645..20884
                     /gene="hfq"
                     /locus_tag="PKZ49_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HND35245.1"
                     /translation="MSTKGQLLQDPFLNILRKEHIPVSIYLVNGIKLQGQIDSFDQYV
                     VLLKNSVTQMVYKHAISTVVPARAVSIPIESSIHE"
     gene            20884..22071
                     /gene="hflX"
                     /locus_tag="PKZ49_01395"
     CDS             20884..22071
                     /gene="hflX"
                     /locus_tag="PKZ49_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HND35246.1"
                     /translation="MHDSKILVVNQRSKALLVGFCQRDSDNESFEELRQLASSAQLDV
                     LEIVKSKRSRPNPATYIGTGKIDEITSLLKTSPEKISIVIFNNDLTPIQERNLTNRLE
                     CTVIDRTSLILDIFAQRAKSHEGKLQVELAQLEHLATKLVRGWTHLERQKGGIGLRGP
                     GETQLETDRRLLAKRVKFLKEKLSILQRQRDVQRLSRKRSQVKTISIVGYTNAGKSTL
                     FNRLTRSNSYAADQLFATLDTTTRKMFIPAYGNLVISDTVGFIRDLPHTLIAAFRATL
                     EETIQADLLLHVVDGTSPVKDDQIEAVNRLLEEINASTIPQILILNKIDQAGSKMRPG
                     YVRDEYGKMTRILISAKTGEGLEYVKLALTEAIRYPSEEVDLEDGFLPEEKEVIDNSA
                     VAF"
     gene            22094..23278
                     /gene="hflK"
                     /locus_tag="PKZ49_01400"
     CDS             22094..23278
                     /gene="hflK"
                     /locus_tag="PKZ49_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HND35247.1"
                     /translation="MGLNDPQWGKRKGNAGPPDLDDVLRNFSKKINSLFGKRGGNNND
                     DEEPSGGSPRKPQGGNIFVIIMLLLVVWFASGFYIINEGQRGIVLRFGKHVDTTQAGL
                     RWHLPFPVEKVETVNVSQVRTVEIGYRNNVRSKVLKESLMLTDDENIIDIQFAVQYIL
                     NSPEKFLFNNRDPESAVLQVAETAIREAIGKSKMDYVLYEGREQVAARTTELMQMILD
                     RYEIGISVSRVTMQNAQPPEQVQAAFDDAVKAGQDRERQRNEGQAYANDVVPKARGTA
                     ARMLEEANGYQQRVISSAEGDVSRFQQVLVEYNKAPAVTRERLYLDMMQQVLSNTSKV
                     LVDQKGGNNLLYLPLDKLIQSDSALANRIPMVAQPQVIQEIPDTSGRTRESFRSRERE
                     VR"
     gene            23280..24158
                     /gene="hflC"
                     /locus_tag="PKZ49_01405"
     CDS             23280..24158
                     /gene="hflC"
                     /locus_tag="PKZ49_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HND35248.1"
                     /translation="MGKFSSALSGILTVLVIAISSSIYIVDEREQAILFQLGEVVDVK
                     TSPGLYFKIPIAQNVRYFDSRILTMDTEEPERFITSEKKNVLVDLFVKWRIVDVKQYY
                     ISVRGDEALAQTRLAQTVNSSMRDEFGNRTVHDVVSGERDKIMEIMRQKANVDAKKIG
                     VEVVDVRLKRVDLPQEVSDSVYRRMEAERKRVANELRSTGSAEAEKIRADADRQREVI
                     LAEAYREAQKIMGDGDGQAAAIYADTFQKSPEFYSFYRSVEAYKQAFKNKTDILVLEP
                     SSDFFKYIKGAAEGKK"
     gene            24250..24435
                     /locus_tag="PKZ49_01410"
     CDS             24250..24435
                     /locus_tag="PKZ49_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HND35249.1"
                     /translation="MWEVFFIAIGLMMIFEGLFPFSFPNAWRETFQKLILLEDNQIRF
                     IGLTSIVVGLIILLLVN"
     gene            24484..>24854
                     /locus_tag="PKZ49_01415"
     CDS             24484..>24854
                     /locus_tag="PKZ49_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HND35250.1"
                     /translation="MRNWILPEYVEDILPAESLHIEKIRRQMLDLMHVHGYQLVIPPL
                     LEYTESLLSGSGSDMDLHMFKVVDQLSGRMLGLRADITPQAARIDAHLLNAQGVTRLC
                     YASSVLHTIPCEIARTREPFQ"
BASE COUNT         6977 a         5264 c         4963 g         7650 t
ORIGIN      
        1 atcatcctca attactactt cttctttgga gatattttct caactgcttt tggtagagat
       61 atctcaccgt gtgccacctg tttagctaac tcaggatcgg ccttagctac tttgtctgcc
      121 atgcgttgag tgcgttcgct tgcaccagat tcgctagctc tatctgctac agtttctaga
      181 ccggcaacgt taccggtctt aattgtgtat ctatttgctc cttgcacttg cgccttagcc
      241 caatcctgtg cactagcaac aatcgcagcc tgttggcctg gcgtcatgtg tcttctgtgc
      301 aagtttgcag acaacacaaa tgaaacaata ttgtcaccat caaaatcaac aaactcaggt
      361 tctatgccag cctcaacgca tgctctataa tacaaattat gtatagcagc tcaaaagtta
      421 acttttaatc atttacatag atgtaagaag tattatattg acgtttttta tgatttagtt
      481 ttttatatac atcaaaatca aaccaacttg ccgtaagtat tttatcttgg cgaatcacaa
      541 tatctagatc tgtgtctatt gcttcataat aagtagtaag gctcacttca aatgtatctt
      601 tatctcgata tccactcatt attggaatta atgaaatttc cttatctaat ccgctacttt
      661 catttatttc tattccggca ttgattccac gcggtgtata tgtgggcatt gaaaaacgtt
      721 tctgaaaaag caattaacca aaaattggtg aattccttta ataatcccag ttatgccgtt
      781 ctcctcgagc atggcaacac caagcataaa gtgcatcata cattaccatt ccgtgtgcaa
      841 gcatttcatg atcatcttta aagttatgtg ataaacctaa cgatattgcc aacaaacctg
      901 cgcactgtgg cgctaaatca aggtgatcag tatctgcacc gcgtactatc gcagctaaat
      961 cttggagcgc tggactggtc aaattatact ttttgagaaa cgcatcaaag ctacattcat
     1021 taccgacatg agataattct acgcctggga tatcgaatgg aattgcgcca gtcaattttg
     1081 cttcagctac gacctggtca ggtggcacaa ataaaaacgc tgattcttta tcgataaatc
     1141 gagcaattaa ccaaggacag gcaatacggt caattttggg gcgttcacga gtaatccatt
     1201 tcatggtttc tttccttatg tgagatttag aatcagtccg atgattgcgc agattgcgat
     1261 gacttcaatc acattgcgtt tatattgaaa caaagctata cctgccaaca aagcaattag
     1321 ggctgatatc caatcaaaat taccggaaaa tccctgtggc cacattacgt gataaccgaa
     1381 aaacaaagcc aaattgagaa taacgcctac aacggccacc gttatagcag ttaatggtgc
     1441 ggtaaattta atattgtcgt gtgttgattc taccagagga ccaccggcaa gaataaagat
     1501 aaaagaaggt agaaatgtaa accaaacgac taagattgca gccattgttc ccgaaataaa
     1561 taacatatct ggtccaagga gggctttcac ataaccacca ataaatgcga cgaaggccac
     1621 taccatgata agcggacctg gtgttgcttc acccagtgct aacccgtcaa tcatttgcgt
     1681 tggtgtaagc cattcataat attcaacggc accttggtaa acatacggca ataccgcgta
     1741 agctcctcca aaagtcatca aagccgcctt agtaaaaaac cagcccattt gagtaaatgt
     1801 gtgatcccaa tcgtagctta tcgttaacaa tatcattggc agcagccaca gaagtccccc
     1861 gattacaact accttaacta atcgaagcca acgaaatttt gcatgttctg gcgttggtgt
     1921 atgatcgtca attaaagcta aaccaaagga tttgtttgct ttactgtgat tactatttac
     1981 tttgaagctg tctggaataa tgcgtccacc cacatagcca agcaatgcag cgctaacaac
     2041 aatgagtgga aatggtagat taaatacaaa gattgcaaca aatgaagcaa ctgcaattga
     2101 ccatagcaaa ctatttttta gcgttcgtga acctagtcga tatgcagctt gcactacgat
     2161 ggcagtaacc gcaggcttta taccatagaa taaaccattt actagagcaa tatcgctaaa
     2221 gctaatataa acccatgaaa gtgcaattaa aataaatagc gacggtagta tgaataatat
     2281 cccggcaatg attccacccc atgttcgatg catcaaccag cctatatata tggctagttg
     2341 ttgagcttcg ggacctggca acatcatgca atagttgagg gcgtgcagaa aacgtcgttc
     2401 tgaaatccag tgcatacgct cgaccaagtc ttggtgcatg atagcaatct gacctgctgg
     2461 tccaccgaaa ctgataaaac caagctttag ccaataccag aaagcttgtg ataaggaaat
     2521 ttttttaggg gcatctgcga tatcaggaaa ttgctgcttt ggtacgtcca ttactcctcc
     2581 ttggtattat ccaattttag agtagtcgtt ggacgatgat cgtcttgcag tacgcgatgg
     2641 ggagactact ttatccccga tttcgctatt gtgtatgtat caaaagtgaa aagcaatagc
     2701 gaactgtaaa agatattatc acaggtgaaa cagcactaat aaaaatcaca actgattgat
     2761 gtatttaacc gacgtggatt caacttcgaa gtgcaacact taacggtgaa ttagttgtag
     2821 tagtctcgac ctgatctgac aattacccga tttaacgatg tgtctgttag atcaatttct
     2881 ttctgccctt cttgcggtag cgttgcgata tcttcagcaa catttataga tatctcgcca
     2941 gcgcgaaccg tttcgataag ctcatccgat ccagttgaaa taatgttctt tactttatag
     3001 atgtttctag aagagacgcc tgataattct gacaattctt cgcaagttct gcctgtttca
     3061 ccctgttgca aatttgcact agggtctgct ttgaccccag ataacattct ttctttcgcc
     3121 ttttcagcaa tcagcggttc ggcctgaagc gctatttcag cacgctgaaa gtcggttaaa
     3181 ttacgacggc caagttggtt cttaagaatc caaagcttta tgctgtagtg atctttgact
     3241 tggtccagac gttgtaaagc ggtatttgaa attctgaatc ttcgtggttg gcgagagttt
     3301 tatgcatttt tgcaaggttg ctgttttgca taaaatcatc aacctttctt tttatagttg
     3361 cctgatcagc accagcaacc tcagcaattt cctcttgcga atgacacgcc atgaacatgt
     3421 caaaaataat ctgattcgtt ttctcgcgct catcgcaata tagcaagaat agcagctata
     3481 gcagtagcaa atcttatttc ttaaaaatct aacatttaaa cagccacggt ttttcgtcag
     3541 tagattgatt agctaatgca cgaaatgcag tcgttgggtg aactgcttgc tttgtaatac
     3601 gaagacgtgt cgcaaaagaa gaggccattt tattttcacg ctcacgcatt ctaagtaatt
     3661 tgtcataatg acgtaacccc tctcttgttt tgagcaggtc tgtgttcaat gtatgaagtt
     3721 ccttattcaa gagcctactt tgtgcaacat gcttacaata catttccaat aaaggtttgt
     3781 gcattggcgt aaagaaatta tttggtacat tactgattac ttcttcccag atcacttttt
     3841 ccgccatact taaagcctca tcaggctcta gccttaaata gcaagattgc agcatgaatc
     3901 cttgctgata attttcttca ctcgattttc tacctcgttt ttccattttt tctccacatt
     3961 tagctaaaaa gagctaggcg cgcggtctac aacacctttt ccgttaaagt tttaatgccc
     4021 cccctgttga ctatatttac actacctctc aaaacaaatc aggaagcgtt ttaagcaatt
     4081 aaggctcatt ttgatacctg cccattaatg ccattatatt aacgcaacac tgttgcattt
     4141 aaagattcaa tttttattaa gtaaatcttt aacctcaaat tcaccattag catgctttaa
     4201 gaaataggcc aaagcctcgt catcggcctt acatttatcc aagacgatat ggtgatattc
     4261 ttctggctct tcgattttat ataaccaggc gcgaacagct tcaatcgatg gtgaattatc
     4321 cgtaatgctg caagtaacct cattattgaa ttcagttaca gcctcatcag gaattgtgtc
     4381 gtaggttttc ttacctttga ttagctctgc aagaatgctc ataacattac ctcatttttt
     4441 agcttggtta gtttgctact gctattcttg ctattgttgc tattttcagc attaaacttt
     4501 tctatctgta gcaggaatag caactgtagc aatagcaaat gctaattatt aaaatctaac
     4561 aggttaggat tgattactaa gattatccgc ttaccatcct tttgcacttg caatcgatca
     4621 agttcagcaa gctcctgagt tgccgcatca agtttttcct ttttacggag tggcccatat
     4681 tgcaatactg cgttctttgc tactaggtgc gtttgctccc gcttacaata atcaatcaac
     4741 cagctatcga gttttgctgc atttgctagc tcttctggta gcgccaattc accaaaaaag
     4801 cgtcgtgatt cagataaata ccacgacaca atacgactag cactatcgaa agcatcaaga
     4861 cttattgcct ggtttggttt gtttaaccat tgaaataatg cggctaaccg tactgcatta
     4921 tcagctgatt tgctggctac atcgcgcacg tcatataact ctttaccatt ggctagctgt
     4981 ttttctatcg tgtcgtgata gctaacccat gcttgctttg cttgaggtga tagttcaagc
     5041 atagtcggtg ttaatgcgcc attttcatca atcggaacag gatcatttaa gattgcggta
     5101 attcgtcgat tgaattcgga aaggtaaggc cagctttgag gcgcttcggt aaacatcctg
     5161 ctaccttgcg ttgattcagg ccatgccacc agaaaacgag caagaaaacc agtgccacgg
     5221 gctaattcac cagatttatt aaagaatgac ttgagcgttg tttcctgaat ttgcaaagcg
     5281 acagtaagac gcgctccttt tacgataaag gactcagaag ttcttcggtc gattgctagg
     5341 ctattaccat cccataattg attgagttgg ctaaggtttt tcataatcga atccttcccc
     5401 atcgcatgcg aaccaaagac aatgcctgcc tcacttgaga tcattccccc cgaaggccat
     5461 tgcttagcca gattgtaggc taacgcttcg ggtgttgtat caacatagag aaggcgtggt
     5521 attcttagcg gctccggttt gctatattca agttgcttca gattttcctt aaagacttcc
     5581 gttgatttgc ttttcttacc tgcttctttt atagctgaca gcaatccttc tctttctgct
     5641 agccatgctg cattctcaga ttcatattct ttgactgtta ttttcatagc ttccgcttgt
     5701 tcctcttggt actgacgaat ggcagaagta aaaaagctat cgcaggtagt tttacgctca
     5761 cctgaatctg caatcgttag tagaaataat ccagtaggcc cttgcagttt ttcagctcgt
     5821 ttagcgtcaa cgtgggcttg gcatgctagc gatagcgaac ccaatgcact tgatgctact
     5881 agcacggtcg gtgccttaac gaatgctcca acttcttcta cagccagtcg gataacatcc
     5941 ggtaatgcgt caataggata tggttctgat tgaattcttg cggtaattgg ttgaggctga
     6001 ggccaaccac tattaattgg gggatcaact gcatcatcaa gattcaattc gccagtattg
     6061 gcaacattgg caaggttatt cgaaaagctt tcagatggag aaatagaatt ttcaatcatt
     6121 tattttttct ccattgttaa ataatcattc caatccatac caggtcttgg tggtattagc
     6181 accttgccac caatagcaat tgctgcttct cttgccttag cctgtccaat gccagacaaa
     6241 tcgttatccc cgcatatgat gatttctgaa tctggcgata attctcggat attctttgct
     6301 accggcaata ggtttccagc atcaaaagca atgacaacac gctgtccgca actcttgaat
     6361 aaactggctc ctgtagcaaa tccttcagca atcagaatac gtggcgacag atcgccaata
     6421 atgtaaaaac agccgtgctt acgtccaccc gaaagaaaac gtttgcagcc ttcaggatcg
     6481 ataaattgca aattaaccag tcgattacct tcgttgtaaa ttgagatagc gagtgcatta
     6541 caataaagtc tagctccgta aggttgaaca gcctttttca ccaaataagg atggtctact
     6601 tgattgttga ctggtttgga tttctgccag atttcagcag cctttttagc tgcttcttcc
     6661 tgcttttggc gtgcttctat ctcgcgctga tgattagctt ctttgaagct cttaattaca
     6721 tcagtggaaa catatgaatt tcctgagcta cgccatttct gagaaatacc acttttgaag
     6781 tcttggaaat aacctgcagg ccggttatct atatgaagtc tataagatcc gtttaagcta
     6841 ccttttttat gaccttcgat atgaaatcga tgcaactgcc catcagggat aatttcacca
     6901 gaataagcta tcccagcagc ctgcatagcc tggcggaatt gtctctttac tacttcagat
     6961 aaattaagca cgatagcctc ccgtgcggtt agctaaagcg atcaagccgt tacgttccga
     7021 ttgttctgct atctttgtcg cataaagcgc accttctggc agcaaacttt cgatatattc
     7081 ccgtaacatc tgccttagga tatgtgctgg cattgcttca gcttcaacag tactttcgat
     7141 ttgctttgat cgaatgtcgg tttttttcct tggctttctc ggtagatcgt attgctcaat
     7201 ttggttttcg ttgattgcaa ttctgcgaag atccatttca atataaccat tgagatgtaa
     7261 ccgtagttca cgctcaagtg attgatcaat caaaacacca gccggatcat aatcaccgat
     7321 gtaatagata atcaaaggcc tgttgtcttt agagcttatg tgctgttctg cagcagcatg
     7381 tgcaaaagat aaactactaa acccactaca tggatataaa tccacagcca gctcattaca
     7441 aaggctgcga atgacacttg caatgcttct gctttctgtc cacacttcgc agcgataatc
     7501 agcatctgac cataaatcag ctctataaag cccagccatg ctcataataa agtcgctagc
     7561 atcaatgaag gtattaacaa aatagccctg cctactcata tctgcaatcc aggcataagg
     7621 aattaagccc gaacggcgca gcttcacaca cctatcttga atatgtcgat aacctttttc
     7681 tgatttttct actggctgat ttaaccttac gtctgtcatc cggtaaaaga tatggcgaac
     7741 actttgtgga tgatcttctt tcagaacttg gataattttc tcatccagtt gatttaattc
     7801 atcttttgtc cttcttttac gttttaacgt gctggcacga taaattgaaa attgagaggt
     7861 tctcataagc ttacctccat gttatgcttc aaattgtcgg tttcttgtct aaggattccg
     7921 tcttctttat cggttggagc ggtggccgcc gctctaacct ctaatacata gcacgctacc
     7981 ctatgtattt ccccacagtc gctttgtcta tctatcttga ttgttttaat gtccaatcct
     8041 tgcttacgca gctccatgac tctagccgcg gggtgcatga tatccagctc tttacgtgca
     8101 gttaacgtat caagtggacg ggtacgaagg aaatctagaa ttcgcttacg ttggataatt
     8161 gttgaatttt catattgggc catggttacg cctccatgcc taatactttc tttatttctt
     8221 ttactggcca agctaaacga ccattgatac gtagcggacg aattaggcca ttttcacgca
     8281 tagcccagca gcggagagtt tgcggttttc ggtttaagta aaaggcagct tcttcagtgg
     8341 aaagcgaggg acgtgtaatt ctttctatgg actgaaaatg atctaaattc gtggacattc
     8401 tgaattacct tctgttgttg ataacaaaag gaattgttcc tgattagcac tatgctggct
     8461 aacgaaaata ttcagatatt tttcatgact tttttgtaaa tccttttaga gatattaata
     8521 aacgccgtcc caagagacga ggaattaaac tcgatagaga taaatcgtaa tagatcttat
     8581 gaattgtttc cttgatcaat attcctacat cttttagaaa tactaaacag cgagtcacgg
     8641 tgattttttg cttttcccaa ccaataatcg actcgatcaa tcttcaggcc accaagatcg
     8701 taaattgctc ttaaacattc agccgctaac ccattattgc tagttgtaaa atcaatacca
     8761 gcttcaaaga atgacttctc caatgctaat gcaactgctg tttctgattt ttgggaatac
     8821 catggataat tatttcctga tgtatttcct acatgcacag gcaaatcttt agcagcctta
     8881 cgcagtccta atgaaaaggc ctttaattct tttatgttat tcttatctcg aagaaattca
     8941 gcttcaagta ttgcatcgaa gctatactga aacggattag caatttttct aatatttgaa
     9001 atttcctcac aaccacgatg aatggcatag cctagtgcag gagaatcaag agcttctatc
     9061 tgattaatta atccttctat atgtgcagcc agtgagttta ttcgctcttg tcttcgttgt
     9121 tgtttttctg gaagaaattt ttctgattca ggaatagctt ctgcaaagca ttcaaaacat
     9181 gagatcatca agcaaatttc tgaatcaggg ttatagttat atgagttctt ttttttaaaa
     9241 gcagatgata gcaactgata taaccttgct tttgttatct catcaaaatg taagcgacca
     9301 ttagaattat ttggcattaa tatcacttca aagtatcaac ccttcaagat aggcctcacc
     9361 aaggcggtga aggtttccgc tcttcgcccc gtcgggttat ggggtgttaa acagttgtaa
     9421 tctatttaat gctttccaat gacttaatat tcggcctagg tcactgcttc gccaaccata
     9481 aatcccactg tgtttgcact cctagccaaa catctggtgt tgtacccagc caagcagaaa
     9541 gcctaagtgc cattgtcgct gttattccag ctttaccgtt tagtaccttc gaaagtgtca
     9601 cacgtgaaat ctgtaattct tttgcggctt gtgttacggt catttcttca ggtaaccatt
     9661 ctcgtaatat ctcgcctgga tgtgctggat tgtgcatttt actcacaggt atttttcctc
     9721 ttatcaatgc tgctttatcc agtcccttaa cgcatcattg atgcgtgttt gccagccatt
     9781 gccgctcgcc ttgaattgct ccaatatatc tgtatctagt cgaagcgtta cctgttcctt
     9841 tttcccgctt ccttgaggtc ttccgcgcac ttttttgaat tgctgcaatt cttcattcgt
     9901 taaaggttga ttatcaggat cagtaagtgc tgctgctgta atctgtgcat cttcttctgg
     9961 tgaaggtaga actaagacgc gacctgattt agtcttgatt tgcatagtga attacctctc
    10021 tataatttgc tttacgtaag ctgattattc gtcgatgatc ttcacgatca acataaacca
    10081 cgcaatacaa cctttcatca attggcgcta aggcgatcat tcgaacttcc ccataatcgt
    10141 gccgcgtgtc ttgccatatc aacgcagcat cccagtcaat gtgcattgca tcgtgcagtg
    10201 atacgccatg cttttcctta tttattgcat ctttagtggc atcgcaaatc actttcatat
    10261 attaatgtag ttacgtttat tgcaagcgtc aaggattatt tgtagctacg tttatttatt
    10321 tacaacgtgc aatcctgctt ttattggtat aaattcaatc ccagcttgct ctaaaatcca
    10381 cgtttctatt ttggtgtgcc atagccttag taaatcgagt gggcgcctaa tgtaattctt
    10441 ctcacgtact ccgcttggtt tgtggccttg gatttgggca gctattcccg ctggtgtttc
    10501 tacccattcc gataaggtag cgaatgaacg ccttaatcca tgcaatgaaa cactcacacc
    10561 aatgattgag catatcttgc tatgttgttt attcggttct ataaggcggc cagttgcagc
    10621 agtggggcta ctgaataccc agtgatttct acggggcaaa ttactcaata actgtgatac
    10681 gtaaggggtt aacgggatca ctctttggcc ttctaccttg tcgcgtatgg tcaaactatt
    10741 ccattgaaag tcgcaatctt cccaacgtag attggctaat tcttcacgac gtgatccggt
    10801 tagtaataag gattgcagat aagcactaat aactgggttt ggaatttgcc tggctgcttg
    10861 aaaccaagca gctaattgtt cgcgttgcaa tgcgtcattt tttgctttgg ctttacctag
    10921 attctcccgt gcagttttac tttgtgctgc gttatcaata acaatttctt tatagcttga
    10981 atgcctgcta caccaaaaca aaaaggcttt taatagtctt aatgcaagcc tagctctagt
    11041 aggtcttttg atcgattcga tttttgccca ttcttcgata cgttcatgag ttaaatcaac
    11101 aaggcgaact ttggctaaag aagaaagtac acccggaaca gttaattctt tactgcgacc
    11161 gcgtatttgc cccccttcat gcattacttt gatgtgatca tttaagtggt gatccgacca
    11221 tagtgactta cgctcattga tgtattcttc ccacgcatca cgaagattta ttttttcggc
    11281 gtgttctctt tttcttttta cttcagcagc tactcttttc tccgcttcta gctggcgagg
    11341 atcaattcct tgatcagtca gcgcttttag ccttgttgct tccttttctg ctctattaag
    11401 cgaccatgag agtacatcgc caatagttaa gcgtaacgtt ctgccatgta attttgtttc
    11461 aaagatataa gacttttttc ctcctttggc tacacgtaaa cctaatcctg gatgatcagc
    11521 atcccaaaaa agactttgcg acttgccaaa ttcacattga aattcagaaa tcactttagc
    11581 tgtaaaacct tttcttttca ttccgtgtag tctccgtgta ggaaatatgt aggaaatttt
    11641 gatcaattat gctaaattta aatcaacacg gattttaaca cgaaatgaca taacttattg
    11701 atattagttg atttagcaat cattttctaa acctaaatca acgtcaataa atgactagtt
    11761 gcgaggactg aaaatccttg tgtcggtggt tcgattccgc ccttggccac caataaccat
    11821 gcggttatta gcgatttttg attagattat tttttccgca aaagctgtgc ataactcgaa
    11881 tcaaagcgaa tcaatggggt cggattcgat tgaatttctt gttttgtcta aattcatctg
    11941 attttctatc tccacccttt gtcagtttat ccgctctgcg gttaagttgc gcttctatct
    12001 tttgcttgaa ataatcactt cctaacaccc aagccttgtt tgtagcttct ctgatgcttg
    12061 ccaagttttg ctcatctatt tgcccgttaa acaaatcacg gtatcgcgct tgtctttgtg
    12121 cctcgtcttg atctaatgca atatacatcg aatgcggcgt aattaacgca tcatattgcc
    12181 ccaaagcatt gctgcgataa ctgctccacg ggtattccga tgggtgctta accatatcag
    12241 cacgtaatgg attaagctct atgtagcgat agcacaacag ggcgtattgt tcgctgtcta
    12301 tcaagctggc tttatagcgt ccttcccata aggtgccggt acgttgatac gtatagttgt
    12361 agtattgcac ataatatcgt cccagcattt gcatcatctt ggcgatgctt tcggctttgt
    12421 gtggggttgc tagcaggtga acatggttgg tcattagcac ataggcgtgg atgtcacatt
    12481 gatgcttttt ggctgcatca gccaacttat ccagaaagaa acggtagtct tcttctgcat
    12541 taaacactgg ctcgcggttg ttgccacgaa taatgatgtg ctggggatgt ccaaccaaaa
    12601 caaaacgggg aaggcgcgcc atggctaata cccctttatc tgttaacaat aggtagccag
    12661 aatagcaggt tatttagaga caatcaatcg agtctgaccc tattgatttg ccttgatttg
    12721 gatttatcga gcccggccct tttgattttc tgcactggcg agcaagccag ttatccgaac
    12781 ctttttgacg cgacctttcc acaaccccca gaagagcgga acctcctcag tcaccacacc
    12841 aacagagaga agcaatccat cagcgtcggt gaccacatac tcaccacgct ggacatcaat
    12901 aggttccatc tccatctctg cttcatgcac cgaagcgcat actgtgacat cgccgttctt
    12961 ctcgataatc gatattggca gcttcatcag aatcgatcac atccacattt gtcggttgat
    13021 cggttatccc acgggcgatg ccgtctgatc gattggccgg gggggggatt tccctttgca
    13081 tcgagaggac caccacgacg gtgatagtgg ggatagcgcc ctgttggatg ccccgtccga
    13141 ttaccccacg gagcaagccg gaagctccta ccgatactca actctgcacc agtcgcgtat
    13201 cctctcgttg tgacagcaac gcctgcagcc attccactca ggtattcagg cgacgagaag
    13261 tcaacaacat cgttggtgcg cattagcgcc cgtatgcccg ccgtcacccc aagtgataag
    13321 ccatccccaa accctgcaca cgcatcgacc aggccttgcg gcagcggatc accccacgcc
    13381 cacaacccta agggatccat aaagctcagc ggattccctc ccacataccc ataagtatta
    13441 attccccctg tcaacccaat cggatcactc tgaatgtatc gaccagtctg tgggctgtag
    13501 tcacggaaat agttgtagtg taagcctgtt tctacatcat agtactggcc aggaaagcgt
    13561 aggtttaccg tggtgacttg cccatttcca tcaggatcct catttggcgt agtattacca
    13621 aaaccatcgc tatgccatga ccaaacaacg ctacccgcat cattagtacc catgcgaggc
    13681 gtgttgagat gatcaacatg gagataggtt aattgggtat caaattgagc cattggtatt
    13741 tcatcagccc agaaatatac tcgaacgaaa ccacctgcat cattcgtctc cgctattaac
    13801 tgcccattga ggtcgtagtg gaagatagtt gtaccattcg gtgatggctt ggtgatccgt
    13861 agaccatttg cgtgataggt aaatggtcca attgtaccaa ccgtttcaag tcgatttgct
    13921 tgattatagg tcatggtcaa accaccttgc gctaacgtat tgcccgcatt gtcgagtaat
    13981 gccgctgcgc tgtcgtacca attcattcgg tttgaattca aatcatagcc atattggatt
    14041 tgactaccat ttttactatg cgttaatcga ttcccattag cgtcatagct ataaatgtaa
    14101 ttctggccta tccctgcatc cgttaacaac cgatctaaac tatcataagt aaaagtttga
    14161 gtccctgtgc tggaagcgcg ggtaagcata ttacccactg cgtcataggt gtaggttgtg
    14221 gaagtgctgc ctaattgcca attctctagc aagcgcatgc cattataact tcgagtctct
    14281 tgcagcccat ttccgaaggt ttgggttcgc aacaatccat ccgcggtata gctaacattg
    14341 ctaatcagtg gttgagcttg cccactgatc gagagattgg cagcgctaat gcgacctaac
    14401 gcatcacggc tatacacgat ggtatggccg gttggataga taatctgcgt tacgcgatta
    14461 tccgcattat aggtaaattg ggtggtatag cttacacctt ctattgtttg tattcgctcg
    14521 acaatattac caaatacatt ataactatag gtggtagtgc cactttcatc ggtaatgcta
    14581 cacaaatgcc caatcccatt aaggcaacta tcataagcat aagttatgtc ttcattacta
    14641 ccagggtagc ctttagtcaa aatacgattt agtgcatcgt atgtcatcgt ggtgattaca
    14701 ccccgtgcat cagtttggcc aatttgatta cctgcttgat cgtagctata agtggtaata
    14761 cctcggtctg gactagtttc gctcagtaaa ttcccaagat catcgtacgt tgatgctgtc
    14821 gttgcaccat tcggtgtatt cagtgaaact acattatctt gcttatcata ggagtattga
    14881 acagggctac tgagggcatc aatagtgcgt gtgagttgat taagtgagtt gtattcatag
    14941 gtagttatat gtgagtttgc atcggttaca ctgcgaagat taccaatcgc atcttggact
    15001 agctgtgtta gcgatccacc aaagttaatg gtttgtaaat tgttacgaac atcataggta
    15061 ttctgaatct tacgcgctag cgtcccactt ggatcattga ttttctcttc aattaaattg
    15121 ccactaagat catatttgta tttgatgcta ttgccgagat tatctgtgac agtgcttaaa
    15181 ttatgggcgt tatcatagct ataattgagc gttgttcctg ctggtgtggt aacactcgct
    15241 aatttaccga tcgtgttata gctgtagctc gtaatacgaa ctatgccaat cggtgaagtt
    15301 tgtttgatct cgataatgcg accaagcgtg tcataggtaa gttgtgtcaa taaccctact
    15361 gcatcggtta ttgaagtgac tcgtcctgct ccatcgtaag cctggtatgt tgtgacttga
    15421 tttaatgcgt tagtaacggt agcaagtcgg ccacattctg cgcctgaggt gcaggaatgg
    15481 taagtaaagg tggttaagtc atttacatca ccacgtggac catcaattga tgtaatttga
    15541 ccatagctgt tgtagttcaa ggtggtaata cgactaaccg ctgcaccccc aggcttatag
    15601 ccagcctggg taattctttc aggaagtaga tcagcgttat agtcaatcgt agtggtagca
    15661 atattgccag atacaacact tggtttcttt atcgaagtga ttaaatcaac cgtatttgaa
    15721 taataagtgt aattggtagt tcgagcgctg gtagtccctg atgcttcggt catgctcaac
    15781 cgttgattag ccgtattata ggtataggtc gtttttcttc cttcttcatc gatatgggaa
    15841 gttaaattac cgcgcccatc gaatgtttgc agaatcttct tgttgtctgc tagattagtt
    15901 ttttctgtta gcttttttac accgagataa ggggtaaatt tgaatacctc tttatttcct
    15961 ttgggatccg ttaccgttgt ttcgtagagg gatccccatg aaaataagaa acgctcttgt
    16021 ggtgctgcat tagttgtttc agcgtgtgca ctggttaatg cgcgaccttg cgcgtcataa
    16081 gtccaagttg caaaccggat accattttgg tcagtgatgc cagtaagatg attcggtaaa
    16141 ctgctgtctt cgtagtgata tgttttactg ctactgtcag ggtaggtaac cgaggttaag
    16201 ttataggcct tatcctgtgc tggttgctgg taagagtaat gcagaacact actgtcgggt
    16261 aaagtagctg tttcaattaa atggtgggtc gcgtcatagg taaattgtag ttgatgacca
    16321 aaagggccag ttactgtggt aagttgtccc gtgctattgt aagcatatgt tgttgtattt
    16381 ccggctgaat cagtctcagc ttggatcaac ccctgcacat tataggtttc gacattaccc
    16441 agacgatcag tcacaatata ctcactggta ttcttgacca aattgaaact ggtatcacta
    16501 tccccagcaa aacttccgtt tgtttgctgc gagaacattt cactcgcacc attcgcccta
    16561 agtaaaatga ttctttcctt gcccgatacc tcacctacta ccaagcgttt atggtaagcg
    16621 gtcatccaac cataccctat accaacattg gcattaaact gagaattgta aaatcgtgtg
    16681 aagggtaaat tagggccggt ataatcctct tcttgctgat gtttgttacc agtgctggtg
    16741 ctacatggat taccccgtga atctgcatga gggcacttaa gggatttatt gggtcctaca
    16801 acatcaaaaa aggtcgtttc ggtaaatata tcaggtttaa ttaagcaagc agtcgcgagt
    16861 gttcctaagc gactataaac aagatagcga cccgtttgat taaaaaggag ttgtgaaggt
    16921 tgagtacatg gcgttatgcc agtttctaga acttgtccaa actcaggaat accgcactcg
    16981 tcccagcttt ttgcaattga tttataaaac cctccaaatg agccatttgg gaattgcaac
    17041 cctgcaaaaa caaaattaat accgcgttca ttattaatac cagtatagca agcagatgaa
    17101 ggaggaattt ctaattgtcg aatctcgcag tgcccatagg cctctgcgcg tataaaatgc
    17161 agtaaacgcg taccaatggg aacaatctgt ccgccttgat aaggtttctg ggtatagtaa
    17221 tctttaaacc aatacgtcag tgattgaccg ccattaactt cgatatctgg gcagcttgca
    17281 ggagacgttg tgtcggtatg atttgctaga gcataattgc tgaaactcaa tgcacataat
    17341 attaacaacg agagaattaa gtgcctgaaa tgatttagca tcgcttagag tactgattaa
    17401 aaacagcgat tattcttaca tcatgacaaa tcaaattcaa tcaataaatt ttacgtattg
    17461 tctgcagagc tattagcagc atgatgtaaa acggtagaac tgatgcaccg agttagtgca
    17521 ataattattt ctaatcaagg ttaagtcgac cttgttactt aaaacaagaa atctaggatt
    17581 ttcactgcaa tgttggtaat gccaacaata aacacaatca acacccataa gaatgtaaca
    17641 actgtagtga tttcagggat gattgatgca gctatcaacc aaataaaagc gcataagagt
    17701 aatttcattt gctatctccc atcattaatt gatgagagag accttgcggc gtgcgttaca
    17761 ctatgtcaac agtataaaag cttgcctgta cttcgatacc atcttaaatt ttatacttaa
    17821 ttaggcatag tattttactt acgcatcaac tcttcaacac agcgtgagca ataaacccag
    17881 aacgtgattc accagcagct tttgcagcag cgtcgatacg tcttaaaaca cgcgatggta
    17941 gcgtaatgtt tactcgttcg attttatccg ataacttggc taaatcaatc gacacaatcg
    18001 cccacacaca gtctttgtat tctggatcat ctttatacgc tttaattgat tttggttcag
    18061 gcacagcgcc gccatcgtca atcactgttt caagccaaag ctcgattgct tcttgagtat
    18121 tgattaatgc ttcttcttct gtatcccctg cagaaaaaca cccaggtaaa tcggggacaa
    18181 tggcactaat cgcatgatca tcatcttcaa agttaagtgc tattggatac ctcatttcta
    18241 ttcctctcca ttattttttt gttctcacgc ctgcttgatt taataattta ttaacaagcc
    18301 ctatccctca gtcttttttg gggtgtggaa ctgtaatatg cccacttctt tctggatgcg
    18361 tatagatatg atgcgaacct ttggtattcc tcagcaccca gccctctttt tctagcagtt
    18421 ttattatttc tttactgttc attgtgtgta ttgtacacac tacaaaccat taatcaatta
    18481 acgggaattt ttatgaattg atttaacaat attagacgag gaattttttc cgcaaagcaa
    18541 aactaggcct agattccatg cggttcttca aggtgcctca aatccttttt tgcggaataa
    18601 aaaatctagt taacgttttg attttattga ttgttagatt agactgaaaa tccttgtgtc
    18661 ggtggttcga ttccgccctt ggccaccagt gttcgatggc attcattcgt taaatcatac
    18721 aatgattgca attcggttta ggaagcccct cctagataaa aacaatcaaa aatacgatga
    18781 agtattgctg taaaatttat cgacatatca tctttttatt cccttaaaat tcaaaaatag
    18841 catttaaaaa agaagcgacc atcagcgcct taaaattttt tgcaaagtga gaaaaacctt
    18901 ccccacaaac cataacacta caaatcacct ataaacccat tttcatgatt tgtagtgagt
    18961 gatccaagct gtcggtttat tttacactcc tctaaccaaa tctagttgat caaacgtaac
    19021 caactgataa atataatgat aatatattgg catgaattct gcttactctt atagagatag
    19081 aaaaataacc attcaattta gttattagga cagaactatg aagaccagta catttaaatc
    19141 aatgcgttta ataagttata cattggctgg tcttttcgtt agtacatcag cacaagcatt
    19201 atttatcaat gcaacggtaa ctggtggaac ccctattctg ggtggaacga ctgatcttct
    19261 taaaagtaat cgcacagttt ctgataataa tatctcaact agcgtttcag catccaaaac
    19321 gcaagcaacg aacgcaacga ttagccaatt cgatgcttct caaggtgttt tagttggggt
    19381 aacgactaca cttacagcag gcaataatac aacctacctt cgtgctgatg gtgcgaatgc
    19441 tgcgcgaggc tcagcgtcag tagacaccag ttggacagga agcggggggt taaatacctc
    19501 aggtaatctt aattctgtta gcaaaagcgg aaataccgat ggtgcagata acagttggaa
    19561 tgcattatca caaactatca gtaatggggc aaacctaaat agctggatag gtgctggaaa
    19621 tttaatctca accatcaata caacgataaa ttctaatcga atcgatgggg gtagtaacat
    19681 tgcgcttatc gctgatatta gtagttctgg ggcaaatatt aatggcgcta cagccccttc
    19741 aattatagac cttagcgcag cttattctgt tacctataat tacatggaac atgcggttgc
    19801 atcgtttgat ggtagttcga ctttaacctc gttggatatt gattttggaa ccattgtact
    19861 gggtgatcta gtcaattcga ttagctttga tatttttaat ctggccggag tcaatggtgt
    19921 tgcgttggat ttgacaagca tttctccaag tggagatacc gctacttttg tgactgacct
    19981 cggatttttc tcagatctca ttcatggcgc tcataatagc tacagtgctt catttgatcc
    20041 taccagtgca ggtacttttg atgcaacgta tttactgacc ttatcagatg atgatgcaac
    20101 gggggcttca gcaagccgcc atacttatag cttagaactc catttaacgg gtactgcagt
    20161 taatccaacg gcgattcccg aacccagcat gatcgcgtta cttggagctg gcttgttaag
    20221 cctgttcggt ttttcaagac gctctagttt aaaataataa atcatttcaa tcattcagaa
    20281 agaaaaagcg ctggtagact atctatcaac gactaccagc gctaagaagt agtccgcaat
    20341 tattcttttc tcctccgtaa attttgcttt tgttccactg ctaacttcca attactgacg
    20401 ttggtacgat tttcagcgat aatccctctt ctttctacat tgcctttact tacaactaaa
    20461 cttagctgag tttaatcgaa gagtcccact tacaaatcga tattaagtaa tttgggccta
    20521 ttgcctttta aataaattag gtttgtttga ttgcttcaga aatgagcaag gtatagattt
    20581 ggatagaaaa ataaagtaga gtctgaaaaa taattaagaa acgattatat aagggggttt
    20641 attaatgagc acaaaagggc aattactgca ggatcctttt ttgaatattc tacgaaaaga
    20701 acatattccc gtatcgatct atttagtaaa tggcatcaag cttcaagggc aaattgattc
    20761 ttttgatcaa tacgttgtgc tactgaagaa ttcggtaacg caaatggttt acaaacatgc
    20821 catatcaact gtggttcccg caagagctgt atcgattcca atcgaatctt caattcatga
    20881 ataatgcatg attcaaaaat actagtggtt aatcagcggt cgaaggcact tttggtcgga
    20941 ttctgccagc gcgatagtga caatgaaagc ttcgaggagt tacgacaact tgcgagcagt
    21001 gctcaactcg atgttttgga aatcgtgaag agtaagcgat ctagaccaaa tccagcaact
    21061 tatatcggaa caggaaaaat tgatgaaatc acttccttgc tcaaaactag tccggaaaaa
    21121 atttcgatcg ttatctttaa caatgacctc acacccattc aagaacgaaa tctgactaat
    21181 cgactcgaat gcaccgttat tgatcgaacg agtttgattc tggatatttt tgcacaacgc
    21241 gctaaaagtc atgaaggtaa attacaagtt gagctggcgc aattggaaca ccttgccact
    21301 aaattagtaa ggggttggac tcatcttgag cgacaaaaag gggggatcgg acttcgaggt
    21361 cctggggaaa ctcagcttga aacagaccgg cgactactgg caaaacgagt taaatttctt
    21421 aaggaaaaat tatcaattct gcagcgacag cgcgatgttc agcggctatc acggaaacga
    21481 tcgcaagtaa aaacaatttc gattgttggt tatacaaatg cgggaaaatc tacattattt
    21541 aatcgattaa ctcggtcaaa cagttatgcc gcagatcaat tgtttgcgac gctcgatacg
    21601 acaacacgta aaatgtttat acccgcttat ggaaatttag tcatttctga tacggttggt
    21661 tttattcgag atttgccgca taccttgatt gcagcattcc gggctactct agaagaaact
    21721 attcaagcag atttattgtt acatgttgta gatggaacca gtccagtaaa agacgatcaa
    21781 attgaagcag tgaataggct tcttgaagaa ataaatgctt caactatccc acagattcta
    21841 atactcaata aaattgatca ggccggttca aaaatgagac ccggttatgt tcgtgatgag
    21901 tatggtaaaa tgactcgtat tctcattagt gctaaaacgg gtgagggact tgaatatgtc
    21961 aagctagcct taactgaggc aatcaggtat ccttcagaag aggtggatct agaagatggt
    22021 tttttgcctg aagaaaaaga agtaatagat aacagcgcag tggctttttg aacgagagaa
    22081 aggattaatt attatgggct taaatgatcc acaatggggg aaaagaaagg gtaatgcagg
    22141 tccacccgat ttggacgatg ttttacgaaa tttctcaaag aaaattaata gtctttttgg
    22201 taaaagaggc ggaaataata acgatgatga agaaccaagt ggtggtagtc ctagaaaacc
    22261 acaaggtgga aatatttttg tcatcattat gcttctgctc gttgtatggt ttgccagcgg
    22321 cttttatatc attaacgagg ggcaacgcgg tattgtgctt cgtttcggta aacacgtaga
    22381 tactacccag gcaggtttgc gttggcatct tccttttcca gttgagaaag ttgaaactgt
    22441 taacgtaagt caagttcgca cagtagagat tggatatcgg aataatgtta gaagtaaagt
    22501 tctgaaagaa tccctcatgt tgacggacga tgaaaatatc atcgatattc agtttgcggt
    22561 tcaatatatt ctgaatagtc ctgaaaaatt cttatttaat aatcgtgatc cagaaagcgc
    22621 tgtattacag gttgctgaaa cagcgattcg tgaggcaatt ggtaaaagta agatggatta
    22681 tgttttatat gaaggcaggg agcaagtggc tgccagaaca actgaactca tgcaaatgat
    22741 tttagatcga tatgaaatcg gtatttcggt aagccgtgtg acgatgcaga atgcgcaacc
    22801 tcccgaacaa gttcaagctg cttttgatga tgcggttaaa gcgggacaag atcgagaacg
    22861 tcaaaggaac gaaggtcaag cctatgcgaa tgatgtagtt ccgaaagcaa gaggtactgc
    22921 agcgagaatg ctagaagaag ccaatggtta tcagcagcgt gttatctcaa gcgcagaggg
    22981 tgatgtcagt cgcttccagc aagtgttagt tgaatataac aaggcacctg ctgttacccg
    23041 tgaacggtta tatttagata tgatgcaaca ggttttatca aatactagta aagtattagt
    23101 tgaccagaaa gggggaaaca atttgctgta tttaccatta gataaattaa ttcaatccga
    23161 ttcagcatta gcaaatcgga ttccaatggt ggcacaacct caggtcatcc aagaaattcc
    23221 tgataccagt ggacgtacac gcgagtcttt tcgcagcaga gaacgagagg tgagataaca
    23281 tgggaaaatt ttcttcagca ttatcaggta ttctcacggt acttgtcata gcaattagct
    23341 cttcaatcta tattgtcgat gaaagagaac aggctatcct atttcaatta ggggaagtcg
    23401 tcgatgttaa aacttcacct ggcctatatt tcaagattcc gattgctcag aatgtacgat
    23461 atttcgattc gcggattctg acaatggata ccgaggaacc agaacgattc attacttcag
    23521 aaaaaaagaa cgttttagtt gatttgttcg tgaaatggcg tattgtcgat gtaaaacaat
    23581 actatattag tgtaagaggc gatgaagcac ttgcacaaac gcgccttgcg caaactgtca
    23641 actcaagcat gcgtgatgaa ttcggtaacc gtactgttca tgatgtagtg tccggtgagc
    23701 gggataaaat tatggaaatc atgcgccaga aagcgaatgt tgatgccaag aaaattggtg
    23761 tagaagtagt cgatgttcgt ttaaaacgtg tagatttacc acaagaagtt agcgattcag
    23821 tttatcgtag aatggaagct gaaaggaaac gcgtagccaa tgagttacgt tcgaccggtt
    23881 ctgctgaagc tgaaaaaatt cgcgcagacg cagatagaca acgagaagtt attctggcgg
    23941 aagcctatcg agaagcccaa aagattatgg gggatggtga tggtcaagct gcagcaattt
    24001 atgcagacac cttccagaag agtcctgagt tttattcctt ctatcgcagt gtagaagctt
    24061 acaaacaagc atttaagaat aaaactgata ttctcgtgct tgaaccaagt tctgatttct
    24121 tcaaatacat caaaggcgct gcagaaggga agaaataatt actaattcac taaaatacta
    24181 tcgatcaatg cgaatgttat taaagagaaa gccattgatc gatagtcaaa gattggagtt
    24241 atttgcttaa tgtgggaagt ctttttcatt gcaattggat tgatgatgat atttgaagga
    24301 ttgtttccct tctcatttcc caatgcatgg cgcgaaacat tccagaagtt gatcctgtta
    24361 gaagataatc aaatacgatt tattggccta acgtctatcg ttgtaggttt aatcattctt
    24421 cttttagtga attagatcag acgctatttc ttcaactgta acttaccttg gctttccctt
    24481 tcgatgcgta attggattct tcctgaatat gtcgaagata ttcttcctgc cgagtcatta
    24541 catatagaaa aaatccgccg gcagatgctg gatttaatgc atgtgcatgg ctaccaactc
    24601 gtcattcctc ctttattaga atataccgag tccttattgt cgggtagtgg tagcgatatg
    24661 gatctacaca tgttcaaagt tgttgatcaa ctcagtggaa gaatgttggg actccgggca
    24721 gatatcacac cacaggcagc acgcattgat gcacatcttt tgaacgccca aggtgttact
    24781 cggctttgct atgccagcag tgtcctacat accataccct gcgaaattgc tcggacacgt
    24841 gaaccttttc aaat
//
LOCUS       DAOQKQ010000011        24410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_848_length_24410_cov_5.962061, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000011 DAOQKQ010000000
VERSION     DAOQKQ010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 24410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24410
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_848_length_24410_cov_5.962061"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..2499)
                     /locus_tag="PKZ49_01420"
     CDS             complement(112..2499)
                     /locus_tag="PKZ49_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HND35251.1"
                     /translation="MNRPVTLEPVSGQNLDINYQLDFSKRLQAVAARINAANNIDEIM
                     LEVSKDICALFNADRLTIYTISEDKSYIVSRVKTGLDSFQDLKLPITEQSIAGFAGLN
                     KKIVNIADVYDRGELMKLSSQLHFLKEVDKRTGYRTKQMLAAPILNVGDNELIGIVQV
                     INNKEDKPFSQIVVAGISEICQILAVAFKQRQKQQYLARSKYDHLISDSIISAAELEM
                     AARNAREKGCDLEDILIDEFQIKPSQIGEALSLYFKVPYEPYRADRIKPMEILKNLKR
                     DFVESNQWLPIDDAKEGLIVLALDPERIKVLRMVNQVFPKHKIVYMVCTHPEFKATLD
                     LFYGGVGSTNLVDTGNINDMLFDLKEGEEEVVVETDETSAASDNELVKLVNKIIMDAH
                     RMGVSDIHIEPYPGKEKTKIRFRKDGSLMPYIEIPASYRNPLVTRIKIMCDLDISEKR
                     KPQDGKIKFKKFAPLDIELRVATIPSAGGLEDVVMRILAAGEPIPLEKMGFTPRNLDE
                     LKTIISKPYGLFFVCGPTGSGKTTTLHSILKYLNTSETKIWTAEDPVEITQKGLRQVQ
                     MNVKAGLTFPVVMKSFLRADPDIIMVGEMRDKETTSIGIEASLTGHLVFATLHTNSAP
                     ESIIRLLDMGMDPFNFSDALLGILAQRLAKRLCSKCKKPHTAGSDEIQSLLAEYCEEL
                     KNIERFRTNPDAAYKRIEGDWIKQYGNEQGQITLYQSVGCDSCNGTGYSGRVGLHELL
                     TASDALKKNIQEHARVADMLVTALSEGMRTLKQDGIEKVLQGITDIHQIRAVCIK"
     gene            complement(2519..3283)
                     /locus_tag="PKZ49_01425"
     CDS             complement(2519..3283)
                     /locus_tag="PKZ49_01425"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HND35252.1"
                     /translation="MKLRVLGCSGGIGKGSSTTSMLLDDDILIDAGTGVSNLTLDELV
                     KIKHIFVTHSHLDHIALIPFLIDTVGCLREFPLIVHAIPPTLEAIQKHLFNWEIWPDF
                     TKIPNGENPYLTYKSLAIGNEVDLEGRKITPLPANHVVPAVGFRLDSGMASLVFTGDT
                     TTNDALWVEVNKIQNLKYLIIEVAFCNAKKDIAMRSKHLCPSMLAEELEKLEQEVEIF
                     ITHLRQGEADLTMQEIERCAGRFNSRRLMSNQIFEF"
     gene            complement(3368..3793)
                     /locus_tag="PKZ49_01430"
     CDS             complement(3368..3793)
                     /locus_tag="PKZ49_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HND35253.1"
                     /translation="MLKMLLPVDGSEACSKAISQFISILGWYKELPTIHLLNVQYPLQ
                     GDISMFISQDNIKQYHQEEGLKDLMQVRKLLDTAKVAYQYHVAVGEPAETIIQFAQAQ
                     QCDQIVIGPRGLGVVTGMLLGSIASKLIHLSDIPVLLVK"
     gene            complement(3840..4562)
                     /locus_tag="PKZ49_01435"
     CDS             complement(3840..4562)
                     /locus_tag="PKZ49_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HND35254.1"
                     /translation="MTIYEPEFWVAALQIIAIDIVLGGDNAVIIALACRKLPEKQRNQ
                     GIFWGVFGAIFIRVLLVFFALNLLTLPFLKIVGALLLIWIGIKLLMPESSDNGHQVDA
                     GTTLLGAIKTIIIADTVMSLDNVIGIAGAAKGDIGLVVFGLLISVPIIVWGSKFVMKL
                     MTRFPVTIVIGAGLLGWIAGDMSTTDTVTKVWIEDHAAYLRWVAPIVTTLIVIITGKW
                     LAARAVRKAMVLTNLGDNNKSI"
     gene            complement(4581..5738)
                     /locus_tag="PKZ49_01440"
     CDS             complement(4581..5738)
                     /locus_tag="PKZ49_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HND35255.1"
                     /translation="MSDHDTTTTNPLPKIGLVLTGGGARAAYQVGVLLAIAEMVPAHA
                     RSPFSIICGTSAGAINAAGLAMSATHFSTGVRQLQKVWCNLHVDMVHRADLLGLIRNT
                     LHLLGSLFSSGLARRPVSLLDNAPLRSMLACRLPFRGIQRSIHAGALHALGITVWGYT
                     SGQSVTFFQGNKSILPWKRSQRVGVASYIGIQHLLASSSIPFIFPAVRVNREYFGDGS
                     MRQFAPLSPALHLGADRVLVIGVNPKDIVTSERIKVTSYPPLAQIAGHAMNSIFADSL
                     NVDLERLNRINETLALIPPNARKESSLTLRPIQSMVIAPSQRINEIALQHAHQLPHTM
                     RYLYRAVGAMGSSGSGLLSYVLFEASFCSALIDLGYRDAMQLRTEILKFIQ"
     gene            complement(5731..5988)
                     /locus_tag="PKZ49_01445"
     CDS             complement(5731..5988)
                     /locus_tag="PKZ49_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35256.1"
                     /translation="MIHTQRQEQELSMLRSEIEMLMSERQSLLRTVGAAAVFIANLDD
                     KILLGDICKSAKVLSASLNDLPEETLSDALEKVNSKFISCE"
     gene            6147..7100
                     /gene="pip"
                     /locus_tag="PKZ49_01450"
     CDS             6147..7100
                     /gene="pip"
                     /locus_tag="PKZ49_01450"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HND35257.1"
                     /translation="MKLFELYPEIEPYNQGMLPLDTMHTMYWEQSGNPSGIPVLFLHG
                     GPGAGSTPQHRRFFDPACYRIVIYDQRGAGKSTPLGEIRNNTTPHLIEDIETLRKHLG
                     IDRWLIFGGSWGSTLALAYGESYPDRCLGFILRGIFLCRKSEIDWFLYGLRTLFPEPW
                     EEFVSELAPSEQKDILATYYTRLMDLDPAIHMPAARAWGRYEGRCSTLLPSSETVSYF
                     SSDGVALGLARMEAHYFKHNIFLPENSLLDNISKLQHHPAIIVQGRYDAVCPIVSAND
                     LHHAWPSAEYLIIDDAGHSAWELGIRSALVEATEKFKSIDR"
     gene            complement(7113..7781)
                     /gene="bioD"
                     /locus_tag="PKZ49_01455"
     CDS             complement(7113..7781)
                     /gene="bioD"
                     /locus_tag="PKZ49_01455"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HND35258.1"
                     /translation="MSIGFFVTGTDTNVGKTLVSCSLIHAFAKTRRSVVGMKPIAAGC
                     ENGRWLDVDQLSAASTRQFDRAMINSYHLLAPVAPQIAANYEAVDIDIAVIQQAFHIV
                     QKAVDIVIVEGVGGFVVPLTSQTDTSDLAVALNLPVVMVVGLRLGCLNHALLTAEAIR
                     ARRLPLVGWVANMIDPQMDDITENIEVLRRRLACPLIGILPYNPHIDASVSANLLDIN
                     PLLM"
     gene            complement(7789..8673)
                     /gene="bioC"
                     /locus_tag="PKZ49_01460"
     CDS             complement(7789..8673)
                     /gene="bioC"
                     /locus_tag="PKZ49_01460"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HND35259.1"
                     /translation="MIYEHSLDKRLLRRSFEKAAETYDHAAVLQREICDRMLSRLDFI
                     KYLPDFILDMGSGTGYGTQKLRERYDRSTIIALDIALNMHLQAYRPISGWRKWISLVK
                     RHSIDYVTGDIEQLPLQKESVNMIWSNLALQWCNDLKQSMTECHRVLKPGGLIMFSTF
                     GPDTLSELRKTFKAVDSYQHINRFIDMHDIGDILVHAGFAEPVMDMEYITLTYQDVAS
                     IMRDLKAIGAHNVMIGRKKGLMGKRAWQQIIDRYELLRQSGKLPATYEVVYGHAWKPE
                     SKLALLKATTKQKLGISE"
     gene            complement(8663..9421)
                     /gene="bioH"
                     /locus_tag="PKZ49_01465"
     CDS             complement(8663..9421)
                     /gene="bioH"
                     /locus_tag="PKZ49_01465"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HND35260.1"
                     /translation="MHRLHVESIGEGPDLVMLHGWAMHSGIWSSVQARLSDKFRLHLI
                     DLPGHGHSAQIPAFSLEQMVEVIATTLPSSCILCGWSLGGQIAIKLALRLPKQINKLV
                     LVATTPSFIKREHWQWGMDEATVQLFMSNLEKQYVQTINRFFTLQMKGSSDSSTALAQ
                     LRNNFFGSVKPNEQSLKVGLQILLTTDLREEVGNLTQPVLLIHGKNDVITDCEAAEWM
                     HQQLTDSKLDLYQRCGHAPFLSYPNQFVASLYDL"
     gene            complement(9461..10630)
                     /gene="bioF"
                     /locus_tag="PKZ49_01470"
     CDS             complement(9461..10630)
                     /gene="bioF"
                     /locus_tag="PKZ49_01470"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HND35261.1"
                     /translation="MLEELVRRLEQRKNDGLFRYRLTRQSPQTPHVKIATQSNLSFAS
                     NDYLGLANHPEVAAALCRGTLQYGVGSGASHLISGHSEVHHELENALAKFVELPNALL
                     FSTGYMANIAVVTTLVGHHDAVFADKLNHASLNDAALLSKAKLIRYPHLDLESLERAL
                     ARTNAPNKLVLTDAVFSMDGDIAPIKALLTLCQKHGAWLLVDDAHGFGVLGRAGKGTL
                     HRGLEPVDLNSPHLIYMATLGKAVGVFGAFVAGQPALIEMLIQFARTYGYTTAMPSVL
                     ACAIMQSLVLLEQEEWRREKLARLIALLKDHYQSSRWPLLASATPIQPLLIRESSTAI
                     LLSELLRREGILVPAIRPPTVPMGQARLRISLSASHHEEEVIRLAETLQRAERTI"
     gene            complement(10634..11629)
                     /gene="bioB"
                     /locus_tag="PKZ49_01475"
     CDS             complement(10634..11629)
                     /gene="bioB"
                     /locus_tag="PKZ49_01475"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041356864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HND35262.1"
                     /translation="MDPILSNIDQKEVNRIDHQQKEMRWNVEQIQILFDLPFNDLIYQ
                     AQSVHRQYHDANTLQLSTLISVKTGGCPEDCGYCPQAARYHTNVEDQDLLSTEEVVSA
                     AKIAKAEGATRFCMGAAWRGPKQRDIVKIAEIIRDVKALGLETCATLGLLKPGQAEQL
                     KQAGLDYYNHNLDTSPEYYQEIITTRDYSDRIHTLEQVRGAGINVCCGGIVGMGESRK
                     TRAALIAELANLDPYPESVPINYLVQVEGTPLYGTESLDLLEFVRTIAVARITMPKAM
                     VRLSAGRRMMPEAVQALCFLAGANSIFYGDKLLTTGNPAIESDKALFEKLGLRTV"
     gene            11724..12488
                     /locus_tag="PKZ49_01480"
     CDS             11724..12488
                     /locus_tag="PKZ49_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HND35263.1"
                     /translation="MSNSNTTLINNRTIIEQLLLPHRCVLCSIQQQGSLCQACKTSLP
                     KLDPNCCTLCLTPLPTFTHCGACLNTPPLWHYIVAGYRYAFPVDVMIQKLKYGLDLTL
                     TPILASFITSKIDRNPLPDAIIPVPLHPEKMKARGFNQAIEISRYVSKQTGIRVLSDI
                     CLRVKNTPSQIELPWKKRQQNVRNAFRCQSDLTNKHIAILDDVMTSGATVNALAKEII
                     KQGATKVSVWVVARSLLSQKSQNNNQSLSQLSDEFL"
     gene            12475..12963
                     /gene="trmL"
                     /locus_tag="PKZ49_01485"
     CDS             12475..12963
                     /gene="trmL"
                     /locus_tag="PKZ49_01485"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HND35264.1"
                     /translation="MSSFNIVLYQPEIPPNTGNIIRLCANTGSSLHLVKPFGFQLNDK
                     QLLRAGLDYHEFINLITHNNWEDCMRSFANKRIFAVSTKGKQRLDQVNFLKEDTFLFG
                     SESRGLPAEILASFPDERKIFIPMLPHRRSLNLSNAVAIIVYEAWRQIGYTAEESSAM
                     TN"
     gene            complement(12982..15108)
                     /locus_tag="PKZ49_01490"
     CDS             complement(12982..15108)
                     /locus_tag="PKZ49_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HND35265.1"
                     /translation="METKAVKKYSQKWLPILQLLLISIFYQNAVAVAETIEGEQSFRN
                     SINAINVVNATNGQITVKITLDQPMSSPPVGFSLTNPDRIVFDFTNSINGLGRNTQEV
                     GNSDLRSINIVQTENRSRLVMNLLRPMTHHAKVQDNHVVIILKAVNLSSVAVTPTLSK
                     IINSSENNLKSLQDIDFRRGANGEGRIEVLLSEPGTAVDVHTQGERIVVEFVKTYLPP
                     NLARRLDVLDFATPVQIIETLTKGDNVQLVVEPKGRWEHTSYQSGTRFFLEVKPVIES
                     EDVPAHKKLMNGGYTGEKLSLNFQNVEVRSVLQVIADFTNLNIIASDSVKGSLTLRLK
                     DVPWDQALDIVLQSHELDKRRVGNVIFVAPREELAAREKMDLESRQQISELEPVQTET
                     FQLNYRTASSIPLTGILSQRGTVEMDDISNTLTVTDIPIRLAEVAKHVKNLDTPVRQV
                     MIEARIVEATETFSRTLGARFGVQNATQMGNRNLGISGNLDHSSGLASGLLSPGGGQN
                     LNVNLPASGLAAAAGGPAALGLSLIKINNGTLVNMELSALETDSRGRVIASPRLVTAN
                     RVEAVIEQGTEIPFQIVSQLGRPQIQFKKAVLSLRVTPQITPDDNVIMRLRVNQDTRG
                     QDTPAGPAIDTKQITTEVLVDNGGTVVIGGIFESADRLNRNQLPLLGDIPVLGNLFKN
                     SLRREDRRELLIFVTPRILNDNLSMR"
     gene            complement(15108..15632)
                     /locus_tag="PKZ49_01495"
     CDS             complement(15108..15632)
                     /locus_tag="PKZ49_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HND35266.1"
                     /translation="MNDRIRLLLIGFVIVLTGCEANTHSDLEEFVNHSGEGLHGTVEP
                     LPEIQTITQFTYAAFELPNPFSPRKNNQVKENQGSIQPDFKRRKEVLESFPLENLAMV
                     GTLQRDRQIYALIKAPDSTLHRVKVGNYLGQNFGLITGISEAEIKLREIIQDSASDWT
                     EHPSTLMLQAQEQR"
     gene            complement(15629..16255)
                     /locus_tag="PKZ49_01500"
     CDS             complement(15629..16255)
                     /locus_tag="PKZ49_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HND35267.1"
                     /translation="MSLLEELQRINPNDPGNWPAKIKAGALIILLCLLIFAGYFFIWE
                     EQWVELENAETEEKTLKENFLTKKKSAVNLVVMQQQLIEIENSLSAMLKQLPNKSELD
                     ALLVDINRAGLGRGLQFELFKPAASELIKEFYAELPVTVRVTGNYHDIGAFASDVSQL
                     SRIVTLHDIEILPGKDQQLVLSATAKTFRYLDDEELASQGKAPSGGKK"
     gene            complement(16272..16838)
                     /locus_tag="PKZ49_01505"
     CDS             complement(16272..16838)
                     /locus_tag="PKZ49_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HND35268.1"
                     /translation="MIRINLLPHRELKRKARQQQFAILAGMTCALGLFIVWGMYEYIA
                     GEIEHQNGRNQYLQNQIAILDKQIEEIKRIKEQKQELIARKGVVEALQLDRAKVVHVL
                     DQVARLMPDGVYLKSLKQTEQHIHLSGYAQSNAWVSTLMRNLDASPWFESPLLIEIKA
                     ATINNSRSNEFDLNVRLSESPNSLTEQL"
     gene            complement(16835..17941)
                     /locus_tag="PKZ49_01510"
     CDS             complement(16835..17941)
                     /locus_tag="PKZ49_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HND35269.1"
                     /translation="MLGKTFNLDLNLFFPQSPRLLGIDISSSSVKIIELSKIKKKSTN
                     TYRLERYVIEPMSKEAIIDGNIQNLEAVGESIRKGWKKTGSRLKEAALALPAAAVITK
                     KVVLPAGQREEDMVFQVETEANQYIPFSLDEVNLDFQVLGPVPDHPEEVEVLIAASRK
                     EKVEDMVAAVQTAGLKAVVMDVEPFAAQAAFELMKHQLPEGGKNQVIALVDIGASITR
                     INIIHDGESVYARDQPFGGDQLTQEIQNQFNLSVEEAEAAKRKGTLPENYGQDVLQPF
                     CETLALEIARAFQFFFTSTQYMQVDCIFLSGGCATIPGLEAIIAARTQTITQIANPFF
                     NMELSNRVSQRQLKQDAPLLLIACGLAMRSFDPS"
     gene            18164..20485
                     /locus_tag="PKZ49_01515"
     CDS             18164..20485
                     /locus_tag="PKZ49_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HND35270.1"
                     /translation="MSLRWLIYPFIALFAVSLIGILLVVFAALVIIPTLPSLEVITDY
                     RPKIPLRIYSAEGLLIGEFGEERRDFVNIRETPVFLKKAILAAEDDRFYQHGGVDYIG
                     VLRAAYSNFSGGGTLQGASTITMQVARNFFLTKEKTLTRKFSEALLAFKIEHSLDKDK
                     ILELYINQIYLGQRSHGFSAAAQTYFGKPLININLAEAAMLAGLPKAPSRLNPVVDLK
                     RSKARQHYVLKRMHELSYISAKEYEEAEKQTILIHRQTRINAMPADHIVEMARQALYQ
                     QYGEDVYTRGLKVYTTIHKADQVAAYEAVRKGILDYDSRYGYRGAEALVDIPPSDVNP
                     AKTLISALQEHSDSDDLLIAAVISSNSNQIEAILKNGQPIQISGDGLKIAKKYLATDP
                     QLGNKRIRKGSLIRVQKNNQNQWKITQLPEVEAALISIDANDGKIRALVGGFDFYLNQ
                     FNHVTQAWRQPGSSFKPFIYSASLEKGFTPATIINDAPIYFDAAQTGSKAWEPKNFDG
                     KFGGPIRMRVALAQSKNLASIRILQAIGPRYAQDYIARFGFDSNRHPPYLPMALGAGS
                     VTPLQMASGYAIFANGGYKVEPYFIERVEDDKGNIVQQVDPLVAGQNATQIIDPRNAF
                     LMNSMMQDVIQRGTATAAKQLKRNDLAGKTGTTSNFIDAWFCGYQKDLITVSWVGFDE
                     PKSLGKNETGGKVALPIWINYMEKALKQIPVATYTPPKGIVTAKINPATGLRETAGGM
                     SEYFFQEHLPPVATVNDSDEDLLSNDDFWSQAF"
     gene            complement(20507..21517)
                     /gene="hemB"
                     /locus_tag="PKZ49_01520"
     CDS             complement(20507..21517)
                     /gene="hemB"
                     /locus_tag="PKZ49_01520"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HND35271.1"
                     /translation="MSPLSQYPQKRMRRLRRDNFSRNLVRENLLSVDDLIYPVFVLDG
                     QHLKESVASMPGVSRLSIDLLLHQAEQCVNLGIPAMALFPVVDASLKSLTAHEALNPE
                     GLVPRTVRALKKHFPELGVITDIALDPYTSHGQDGLIDENGYVLNDETILVLEQQAVV
                     HAQAGADIVAPSDMMDGRVAAIRKKLDDNQHIHTRILAYSAKYASSFYGPFRDAVGSS
                     ANLKSGNKYTYQMDPANSDEALWEVGLDLQEGADMVMIKPGMPYLDIVRRVKEKFGAP
                     TVVYQVSGEYAMLKAAAINGWLNEEACVLEALLAFKRAGADAIMTYFALDAAGWLKQS
                     GS"
     gene            complement(21719..22318)
                     /gene="yihA"
                     /locus_tag="PKZ49_01525"
     CDS             complement(21719..22318)
                     /gene="yihA"
                     /locus_tag="PKZ49_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HND35272.1"
                     /translation="MSILQGIRFYTTVNHLKDLPKTTNIEVAFAGRSNAGKSSAINTL
                     TRRNRLAFVSKMPGRTQHINYFQLNDGNFLVDLPGYGYAKVPQLIREHWRHLLSTYLQ
                     SRSNLVGLILIMDVRHPLTELDQKMLEWFSITNKPVHILLSKSDKLSRNQSMKTLKSV
                     QNFLLEQYPHCTAQLFSNLATVGVEEALQVLSNWFKSTD"
     gene            complement(22333..23097)
                     /locus_tag="PKZ49_01530"
     CDS             complement(22333..23097)
                     /locus_tag="PKZ49_01530"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HND35273.1"
                     /translation="MTYLLVIDSGNTAIKWGLADKDKWIDKGVAMQTHRDSLGGVWGK
                     IPEPSQITISNVAGSLAEQSLMLIFKQLQWTAKVNWAFSKSDQCGVKNYYDIPSQLGS
                     DRWAALIAAWSMKQQACLVVSVGTTMTVDILSNQGEFMGGIILPGFDLMQSTLINQTA
                     LLTDSSNGCFQKVPRNTQDAMVSGAIYGLTGAIEQMYSIFTKSLSHSNPKCIISGKGA
                     QTVLPYIDIAGKEWVDDLVLEGLRIISLTDSSAIFT"
     gene            complement(23112..24098)
                     /gene="birA"
                     /locus_tag="PKZ49_01535"
     CDS             complement(23112..24098)
                     /gene="birA"
                     /locus_tag="PKZ49_01535"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HND35274.1"
                     /translation="MKPISFAILKLLSDGQFHSGTQIAQFLGVSRTSVSNALRGIDEF
                     GVTLYRVTGKGYRWINPVQWLDKNAINQYLAEYSQRFRITIDEVVESTNTILLNTSKA
                     KNYDQSMIDVVVTELQTKGRGRRGRSWFSGLGDSLTFSLSWQFQQGVNFLAGLSLVVG
                     IAIIRTFTSLGIREVALKWPNDLLFNSSKLGGVLIELQGEVQGPATAVIGIGINLTLS
                     DKTKLYVDQDISDLYSITHTPIDRNKLLASLLIEMATVLEIFAAEGFSPFKEEWISYH
                     SYEAREVEVLLPAGLIERGIVTGITEDGSLRLQMESGLKSFHCGEISLRKIG"
     gene            complement(24260..24335)
                     /locus_tag="PKZ49_01540"
     tRNA            complement(24260..24335)
                     /locus_tag="PKZ49_01540"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24299..24301),aa:Glu,seq:ttc)
BASE COUNT         7096 a         5640 c         4550 g         7124 t
ORIGIN      
        1 ttttcaatag atgactgttt atcgtcagat ttctgagcta attttcgcat tttcaaatcg
       61 cctccatccc aataacctta tggaaattgt ggcaattgta aagataatga tttatttaat
      121 gcaaacagct cttatttgat gaatatcagt aattccttgc aatacttttt caatgccatc
      181 ttgctttagc gtgcgcattc cttcacttag cgcggtaact aacatatctg caacacgcgc
      241 atgctcctga atattttttt tcaacgcatc actggcggtt agcagctcat gcaaaccgac
      301 cctgccagaa tacccggtcc cattgcaact atcacaaccg actgactgat aaagcgtaat
      361 ttgcccttgc tcattaccat attgcttaat ccaatccccc tcaatccgtt tgtaagcagc
      421 gtcaggattt gttctaaagc gctcaatatt cttgagctct tcacagtatt ccgctaaaag
      481 tgactgaatt tcatcgcttc ctgccgtgtg gggctttttg catttactgc ataaccgctt
      541 tgccaggcgt tgcgctaata tgcccaatag tgcatctgaa aaattaaatg gatccattcc
      601 catatctagc aaacgaataa ttgactctgg tgcgctgttt gtatgtaacg tagcaaaaac
      661 caagtgcccg gtcagtgaag cctcaatccc gatactcgtt gtttctttat ctcgcatctc
      721 accgaccata ataatgtcgg gatctgcacg caagaatgat ttcatcacaa ctggaaaagt
      781 caaacctgct ttgacattca tctgaacttg cctcaatcct ttctgggtaa tttcaaccgg
      841 atcctctgct gtccaaattt ttgtttcaga tgtattcaaa tattttagga ttgaatgtaa
      901 cgttgtagtc ttccctgaac cagtcggtcc acaaacaaaa aataaaccat agggtttact
      961 gataattgtc ttaagttcat caagattacg tggcgtaaag cccatttttt caagtggtat
     1021 cggttcacct gcagcaagaa tacgcatcac tacatcttct aagccacctg cagaaggtat
     1081 cgttgcaacc ctcaactcaa tatcaagtgg cgcaaatttc ttaaatttta tttttccgtc
     1141 ttgaggtttt cttttctctg aaatatctaa atcacacata atcttgatac gcgttaccaa
     1201 cggattacga taacttgcag gtatctcaat ataaggcatt aatgaaccat ctttgcgaaa
     1261 gcggattttt gttttctctt ttcctggata tggttcgata tgtatatctg aaacccccat
     1321 tcgatgcgca tccataataa ttttgttgac caacttgaca agttcattat ccgatgcagc
     1381 tgatgtctca tccgtttcaa caacaacctc ttcttctccc tccttgagat caaaaagcat
     1441 atcgttgata ttgcctgtat ctacaaggtt tgtactaccg actcccccat aaaacaaatc
     1501 aagcgttgct ttaaattctg ggtgcgtaca taccatgtaa actatcttat gcttaggaaa
     1561 aacctgatta accatccgga gcactttgat tctttccgga tctagcgcaa gcactatgag
     1621 accttctttt gcgtcatcaa taggaagcca ctgattactc tccacaaaat cacgtttaag
     1681 atttttcaga atctccatcg gcttgattcg atcagctcga taaggctcat agggtacctt
     1741 aaaatacaaa gataacgctt cgccaatctg tgaaggttta atttgaaact catcaattaa
     1801 aatatcttca aggtcacagc ctttctctcg agcattcctt gcagccattt ccagctctgc
     1861 agctgaaata attgaatctg aaatcaagtg atcatatttt gaacgagcca agtattgttg
     1921 cttctgcctt tgcttaaaag ccactgctaa tatttggcaa atctcactta tgcccgcaac
     1981 tacgatctgt gaaaacggct tgtcctcttt attgttaatt acctgaacaa ttccgataag
     2041 ctcattatcg cccacgttta aaatcggagc tgctagcatt tgcttggttc gataaccggt
     2101 tcgcttatca acctctttta aaaagtggag ttgtgagctg agtttcatca actcaccacg
     2161 atcatataca tctgctatat taacaatttt tttatttagg ccagcaaagc cagcgatact
     2221 ctgttcggtg atcggtaatt tcaagtcttg gaatgaatct aatcccgttt tgacccgtga
     2281 aacaatgtaa gacttatcct cactgatcgt ataaatcgtc agccgatccg cattaaacaa
     2341 cgcgcaaata tctttgctga cttcaagcat gatctcatcg atattattag cagcgttgat
     2401 tcttgccgca actgcttgca aacgtttaga gaagtctaat tgataattga tatctaaatt
     2461 ctggccagat actggctcta gcgtaaccgg tctgttcatg gttgctttat ccaaagtgct
     2521 aaaattcaaa aatctgatta ctcattaatc ggcgtgaatt aaatcgccct gcacatcttt
     2581 caatttcttg catcgttaga tcagcctcgc cttggcgcag atgagttata aagatttcca
     2641 cctcttgttc taatttttcc aactcttcag ctaacatgct tgggcataaa tgttttgatc
     2701 gcattgcgat atctttcttc gcattacaaa acgcaacctc aataattaga tatttcaagt
     2761 tttgaatctt atttacctct acccataacg catcatttgt agtggtatca ccggtaaaaa
     2821 ctaaactagc cataccagaa tctaaacgga atccaactgc cggcacaacg tgattagcgg
     2881 gtagtggcgt aatcttcctt ccctctaaat caacttcatt tccaattgct aaagatttgt
     2941 aagtcaaata ggggttttca ccattaggaa ttttggtaaa gtcaggccaa atttcccaat
     3001 taaaaagatg tttctgaatt gcttctagcg taggaggtat ggcatgaaca atcagtggaa
     3061 actctcgcaa acaacctact gtgtcaataa gaaaaggaat cagtgcgata tgatctaagt
     3121 gagagtgcgt aacaaagatg tgtttaattt ttaccaattc atccaaagtc aaattgctaa
     3181 cacccgttcc cgcatcaatt agtatgtcat catcaagcaa cattgatgta gtacttgatc
     3241 ctttgcctat ccctccacta cagcctaata ctctaagctt caaaatatat cccccttatt
     3301 cgaatattta acctgatttt tagatatttg ctttcgataa taagcaggga attacattgg
     3361 attctaatta tttgactaac agaactggaa tatcagacag atggatcagt ttgctagcaa
     3421 tagaacccag taacatacct gttactacgc ctaatcccct tggtccaata acaatttgat
     3481 cacattgctg tgcttgggca aattgaataa tggtttcagc aggttctcca acagcaacat
     3541 gatattggta cgcgactttt gctgtatcca acaatttacg cacttgcatc aaatccttta
     3601 atccctcttc ttgatgatat tgtttgatat tgtcttgact aataaacatt gaaatatcac
     3661 cttgcaatgg atattgcacg ttcagtaaat gaatggtcgg tagttcctta taccatccaa
     3721 gaatagagat aaattgcgaa attgctttac tacaagcctc agaaccatcc acgggtaaca
     3781 gcatttttaa catgacattt cctcaaatgt tgtgtgattt ttcacagaat ccaaaatcat
     3841 caaatcgatt tattattatc accaagattc gttaatacca ttgccttcct gactgcacgg
     3901 gctgccagcc attttccagt aataattaca atcaatgtcg ttacaatcgg tgcaacccaa
     3961 cgcagatacg cggcatgatc ttctatccaa actttggtaa cagtatccgt tgttgacata
     4021 tcacctgcaa tccaacctag caaaccagcc ccaatcacaa tagttacagg aaaccgagtc
     4081 atcaacttca taacgaattt acttccccaa actatgatgg gcacactgat aagtaatcca
     4141 aaaacaacaa gaccaatatc accctttgca gcgcctgcaa tgccaattac attgtccaaa
     4201 ctcattacag tgtctgcgat aatgatcgtt ttaatagcgc caagcaaagt tgtcccagca
     4261 tcgacttgat gcccattatc cgaagactct ggcatcaata acttaatacc aatccatatc
     4321 aatagtaatg cgccaacaat ttttaaaaaa ggcagcgtca aaagattcag tgcaaaaaat
     4381 actaatagga cacgtataaa aatcgcccca aaaacacccc agaagattcc ttgattacgt
     4441 tgtttttctg gtagtttgcg gcaggccaag gcaataatta ccgcattatc cccgcctagc
     4501 acaatatcaa ttgcaataat ttgtagtgcc gcgacccaaa attcgggctc ataaattgtc
     4561 attttgttat ttccttcaga ttattgtatg aatttaagaa tctcagttct taactgcatt
     4621 gcatcacggt accctaaatc gattaaagcg ctacaaaaag aagcttcaaa taaaacatag
     4681 cttaataacc cagacccact agaacccatt gcacctaccg cacgatagag gtagcgcatt
     4741 gtatgcggaa gctgatgcgc atgttgcagt gcaatctcat tgatcctttg gctaggcgca
     4801 atcaccatcg actgaatggg tcgcaatgtc agactgcttt cctttctagc atttggagga
     4861 atgagtgcga gcgtctcatt aattctattc aaacgttcta aatcaacatt taaactatca
     4921 gcaaagatac tgttcattgc atgtccggct atttgggcga gcggtgggta gctcgtgact
     4981 ttgatccgtt cactagtaac aatatctttg gggttaacac cgatcacgag cacgcgatca
     5041 gcgcctaaat gcaatgcagg actaagcggt gcaaactgac gcatggaacc atccccaaaa
     5101 tactctcgat ttacccgcac ggctggaaag ataaatggaa ttgatgaaga agcgagcaag
     5161 tgctgaatgc caatatagga agccacgcct acgcgttgcg aacgtttcca aggaagaata
     5221 cttttgtttc cctgaaaaaa cgtaacggat tgccctgagg tatatcccca tacggtaatc
     5281 cctagtgcat gcaaggctcc tgcatggata cttctctgaa tacccctaaa tgggagccga
     5341 caagccaaca ttgatcgtaa tggagcatta tccaatagcg atacgggtct ccgagctaat
     5401 ccactcgaaa ataaagaacc taataaatga agggtatttc gtatcaatcc cagcaaatct
     5461 gcacgatgca ccatgtcaac atgcaaatta caccaaactt tctgcagttg cctgacccca
     5521 gttgagaaat gtgtcgctga catggccaaa cctgctgcat taattgctcc agcagaagtg
     5581 ccacagatga tggaaaatgg gctacgcgca tgcgccggaa ccatttcagc gatagcaagc
     5641 agtaccccaa cctggtaagc agcgcgcgcg ccgccacccg tcaatacaag gccaattttg
     5701 ggtaatggat tagtggtagt ggtatcgtgg tcactcacag ctgatgaatt tagaatttac
     5761 cttttcaagt gcatcggata atgtttcctc aggcaaatca ttcaagctag ctgataatac
     5821 cttcgccgat ttacaaatat cgcctaataa tattttatca tcaagatttg caataaatac
     5881 tgctgctgca cctactgtcc ttaaaaggct ttgtcgctca ctcattagca tctctatctc
     5941 tgagcgtaac attgataatt cctgctcttg tcgttgtgta tgtatcataa attacttcct
     6001 atgcctggta tactaagatg aacttaacaa caaactataa cagtatcttc tatcccttaa
     6061 acagccaagc tctttggaat ttaatcgtct tttcgaattc ctttgaaatc gatcatcacc
     6121 aacaaacaat ttaataacaa ccttaaatga agctatttga actatacccg gaaattgagc
     6181 cttataatca gggcatgtta ccgcttgaca ccatgcatac tatgtattgg gaacaatctg
     6241 gtaacccatc tggcatccct gttttatttt tgcatggggg acctggtgca ggatccacac
     6301 cgcagcatcg tcgtttcttc gacccagctt gttatcgaat tgttatatat gatcaacgag
     6361 gcgctggcaa atccacgccc ctcggtgaaa tccgtaacaa caccacgccc catttaattg
     6421 aagatattga aacactccgg aagcatcttg gtattgaccg ctggttgatc ttcggcggat
     6481 catggggaag cactttagcg ctagcctacg gagaaagcta tcctgatcgc tgcttgggct
     6541 ttattttacg aggaattttc ttatgccgga aaagtgaaat agattggttc ctctatgggt
     6601 tacgcactct ctttccggaa ccctgggaag aatttgtatc ggaattggct ccatcggaac
     6661 aaaaagatat actggctacc tattatacgc gtctaatgga tcttgatcct gctattcata
     6721 tgcctgcggc ccgagcttgg ggacgctatg agggtcgttg ctccacttta cttccaagct
     6781 cagaaacagt aagttatttc tcaagtgacg gtgttgcgtt agggttggca cgtatggaag
     6841 cacactactt caaacataat atctttctgc ctgaaaactc attactcgac aatataagca
     6901 agctccaaca ccaccctgca atcattgtac aaggtcgata tgatgcggtt tgtccaatcg
     6961 ttagcgcaaa tgatttgcat catgcctggc ctagtgccga gtacctcatt attgatgatg
     7021 caggacattc tgcctgggaa ctagggattc gatccgcatt agttgaagca acagaaaaat
     7081 tcaaatcaat cgatcgataa tatttgattg cttcacatta gaagcgggtt tatatccaac
     7141 aaatttgcag atacactcgc atcaatatgc gggttatagg gtaatatccc aattaatggg
     7201 catgccagtc tgcgcctcag cacttcaata ttctctgtaa tatcatccat ttgggggtca
     7261 atcatattgg ccacccatcc tactaaaggt aaacgtcttg cacgaatcgc ttcagcagtc
     7321 aacaaagcat gattcaaaca accgagacgc aatcccacca ccataacgac cggtaaattt
     7381 aatgcgaccg ccagatcact cgtatccgtc tgagaagtta acggaacgac aaacccaccg
     7441 acgccttcga cgataacgat atctaccgct ttctgcacaa tatgaaatgc ttgttgaatc
     7501 actgcaatat cgatatcaac cgcttcataa tttgctgcaa tttgaggtgc aactggcgcc
     7561 aataaatgat aagagttaat catagcgcga tcaaattgcc ttgtagatgc tgcacttagc
     7621 tgatcgacat caagccatcg accattttcg caaccggctg ctatcggttt cataccgaca
     7681 accgatcgcc tcgtttttgc aaaagcatga ataagcgagc agctaaccag tgtttttcct
     7741 acattggtat cagttcctgt cacaaaaaaa ccaattgaca ttacactatt attcgctgat
     7801 tccgagtttt tgttttgttg tagctttaag caaagctaat tttgattcag gtttccaggc
     7861 atgaccataa accacttcat acgtcgcagg caatttacca gattggcgta gcaattcata
     7921 acgatcgatg atctgctgcc atgctctttt tcccatgagg ccttttttac gcccaatcat
     7981 aacattatgg gcgccaatcg ctttgaggtc acgcatgata ctagccacat cttgataagt
     8041 cagcgtaatg tattccatat ccatgactgg ttcggcaaaa ccggcatgaa ctaatatatc
     8101 gccaatatca tgcatgtcga taaaacgatt aatgtgttgg tatgaatcaa ccgctttgaa
     8161 ggtttttctc aattcggata aggtatcggg accgaaagta ctgaacatga tcaatccacc
     8221 cggcttcaag acacgatgac actccgtcat ggattgcttt aaatcattgc accactgcag
     8281 cgccaaattc gaccaaatca tattaacgga ttctttctgt aagggtaatt gttcaatatc
     8341 accagttaca taatcgatag aatggcgttt taccagtgaa atccatttcc tccaccctga
     8401 aattggtcgg taagcttgca aatgcatatt taaagcaata tcaagtgcaa taattgtaga
     8461 acgatcatag cgctctcgta gcttttgagt accataacca gtcccactcc ccatgtctaa
     8521 gataaaatca ggaagatatt taatgaaatc gagcctagat aacattcggt cacatatttc
     8581 acgctgtaaa accgcagcgt gatcatacgt ctcagctgct ttttcaaatg atcgtcgcag
     8641 taaacgctta tctaaactat gttcataaat catacaagct agctacaaac tgattagggt
     8701 aagataaaaa gggagcatgc ccacaacgct gatataaatc caattttgaa tcggtcagct
     8761 gttgatgcat ccattctgca gcctcacaat cggtgattac atcatttttt ccatgaatta
     8821 atagaacagg ttgcgttaaa ttgcctactt cttcacggag atcggtggtt aataaaattt
     8881 gtagtcccac tttaagcgat tgttcattag gtttcacaga tccaaaaaaa ttatttctga
     8941 gctgcgctag tgccgtcgat gaatcggagc tccctttcat ttgcaaagta aagaaccgat
     9001 tgatggtttg aacatattgc ttttctaagt tgctcataaa aagttgaact gtcgcttcat
     9061 ccattcccca ttgccaatgt tcgcgtttta tgaagctggg tgtcgtagca actaaaacta
     9121 atttattaat ttgcttgggt aaacgtaaag caagtttaat cgcaatctgt ccgcctaatg
     9181 accaaccaca taaaatgcat gatgaaggca gcgttgttgc aatgacttca accatttgtt
     9241 caagcgaaaa agcaggtatc tgcgcactat ggccgtgccc tggtaaatcg attaagtgca
     9301 aacgaaattt atcggagagc cttgcctgca cactactcca gataccgcta tgcatggccc
     9361 aaccatgtag catcacaaga tctggccctt ctccaatcga ttccacatgc aaacgatgca
     9421 taacttatta ctacttacct tattctcatg ctgatcttat ttaaatcgtt cgctccgcac
     9481 gctgcaatgt ttcagccaag cgaatgacct cttcttcgtg atgactcgct gatagggaaa
     9541 tacgcaaacg agcctgaccc attggaaccg ttggcggtcg aatcgctgga accagtatcc
     9601 cttctctccg cagtagctca cttaacaaaa tcgccgtact actttcccta attaacaatg
     9661 gttgaatggg tgttgccgaa gctagcaatg gccaacgaga agattgataa tggtccttta
     9721 ataaggcaat gagtcgagca agtttctctc gcctccattc ttcttgctcc agcaaaacca
     9781 ggctctgcat gattgcacaa gccagtacag aaggcattgc tgtggtatat ccataagtac
     9841 gtgcaaactg gatcaacatt tcgattaatg caggctgacc ggctacaaat gcaccgaaaa
     9901 cccctactgc tttacccagt gtcgccatat agatgagatg gggcgagttt aaatcaaccg
     9961 gctccaggcc acgatggagc gttccttttc cagcacgacc caacaccccg aaaccatgcg
    10021 catcatcaac caataaccaa gctccatgtt tctgacagag cgttagcaac gctttaatcg
    10081 gtgcaatatc tccatccata ctaaatacag catcagttag tacgagcttg ttaggagcat
    10141 ttgttcgagc aagcgctcgt tcaaggcttt ctaaatcgag atggggatac cgaatcaact
    10201 ttgctttaga aagcaatgcg gcatcgttaa gtgacgcatg gttcagcttg tctgcaaata
    10261 ctgcatcatg gtgccctact aaggtcgtta caacggcgat attggccata taaccggtag
    10321 aaaataatag tgcattgggt aactcgacaa acttagctag tgcattctcc agttcatggt
    10381 gaacctccga gtgcccgctg atcaaatgcg aagcaccgga acccacacca tattgaagcg
    10441 tgcctcgaca aagcgccgca gcgacttctg gatggttagc caaccctaaa taatcattac
    10501 tagcgaaaga aagattagat tgcgttgcta tttttacatg gggtgtttgt ggactttgtc
    10561 gcgtcaaacg atagcgaaac aatccatcat ttttgcgctg ctctagcctt cgaaccagtt
    10621 cttcaagcat tgcctaaaca gtgcgcaagc ctaatttctc aaatagcgct ttatccgatt
    10681 cgatggctgg atttccagtg gttaataatt tatccccata aaaaatagaa ttagcgccag
    10741 ccagaaaaca aagtgcctga accgcttccg gcatcatacg tcgacccgct gatagcctca
    10801 ccatcgcctt aggcatggtg attcgcgcaa cggcaatggt acgaacaaat tctaataaat
    10861 cgagcgactc cgtaccataa agcggtgttc cctcaacctg tacgagataa ttaataggca
    10921 ccgactccgg gtaaggatca aggtttgcca attcagcaat taatgccgca cgggttttgc
    10981 gggattcccc cattccgaca attccaccgc aacaaacatt gatccctgct cccctgacct
    11041 gctccagtgt gtggattcga tcactatagt cacgtgttgt aataatttct tgatagtact
    11101 cgggcgaggt atctaaatta tgattgtaat aatcgagacc agcctgcttg agttgttcgg
    11161 cttgacctgg tttgagcaag cctaatgtag cgcaggtttc aagcccaagt gctttaacat
    11221 cgcgaattat ttcagctatt ttaactatat cgcgttgttt aggtcctctc caagcggctc
    11281 ccatacaaaa tcgtgtagcg ccctctgctt tggcaatttt tgcggccgaa accacttcct
    11341 ccgttgaaag caggtcttga tcttcaacat tggtatgata gcgcgcagct tggggacagt
    11401 acccacagtc ctcagggcac ccccctgttt taactgatat caaagttgaa agctggagtg
    11461 tgttagcatc gtgatattgg cgatgaacag actgcgcctg atagattaaa tcattaaagg
    11521 gtaaatcaaa tagaatttga atttgttcga cgttccatcg catctctttc tgctgatgat
    11581 cgattcgatt gacctctttt tgatcaatat tacttaaaat gggatccata ctataaataa
    11641 atcagttaat aaatgactaa gaattctctt atgttagcgg gtaagaattg aattgcaccg
    11701 aatcatcacg gattaaacac tgattgtcaa attctaacac cacattaatt aacaaccgaa
    11761 caataattga gcaattattg ttgcctcatc ggtgtgtatt atgctcgatt cagcaacaag
    11821 gcagcctatg ccaagcatgc aaaacaagcc tacctaaact cgatccaaac tgctgtacac
    11881 tttgcttaac tcctttacca acttttacgc actgtggagc ttgtctcaat acaccaccac
    11941 tgtggcacta tattgtcgca gggtatcgtt atgcctttcc agttgatgtt atgattcaaa
    12001 aactgaaata tgggctggat cttaccttaa cacctatttt agctagtttt attacatcga
    12061 agatcgatcg taatccactt ccagatgcca tcattccggt tccacttcac cctgagaaaa
    12121 tgaaagccag aggattcaat caggctattg agattagtcg ctacgtctct aaacaaaccg
    12181 gtattcgagt tctctccgac atttgtttac gtgtcaaaaa tacaccttca caaatcgaat
    12241 taccctggaa aaagagacaa caaaatgttc ggaatgcttt tcggtgtcaa tccgatctaa
    12301 cgaataaaca tatcgctata ttggatgacg taatgacaag cggcgctaca gttaacgctt
    12361 tagccaaaga gataatcaag caaggcgcta ccaaagtcag tgtatgggtc gtagcacgat
    12421 cacttctttc ccagaaatcc caaaataaca atcaatcatt atcacagtta tctgatgagt
    12481 tcctttaata tcgttctcta tcaaccggaa attccaccca atactggcaa tatcattcga
    12541 ctttgcgcaa atacaggatc aagcctacat cttgtcaagc catttggttt tcaattgaat
    12601 gataagcaac tgcttcgggc cggattggac tatcacgagt ttatcaatct gatcacgcat
    12661 aacaactggg aagattgcat gcgtagcttt gcaaacaaaa ggattttcgc cgtttcgacg
    12721 aagggaaaac aacgcttgga tcaggttaac tttcttaagg aggatacctt tcttttcggt
    12781 tcggaaagcc gggggttacc ggctgaaata ctggcaagct tccccgatga gcggaaaatt
    12841 tttattccga tgctaccgca tcgacgcagc ttaaatctct cgaatgcagt cgccattatt
    12901 gtttatgaag cttggcgaca aataggttat acagcagaag aatcatcagc gatgactaat
    12961 taatgaaacc caaacttcct gctaacgcat tgataaattg tcattgagaa tacgtggtgt
    13021 aacaaaaata agtaattccc gccgatcttc ccgtcttaaa ctatttttga ataagttacc
    13081 caagactgga atatcgccca acaagggtag ttgattgcga ttcagtcgat ctgctgattc
    13141 aaaaataccc ccaataacca ctgttccccc attatcaacg agcacttctg tagtgatctg
    13201 tttggtgtcg atcgctgggc ctgctggtgt atcttgtcca cgtgtatcct gattcacacg
    13261 caatctcatg atcacattat catccggcgt gatttgcggg gttactctaa gactaagtac
    13321 agctttctta aactgaattt gcggccttcc gagctgactc acaatctgga atggaatttc
    13381 ggtaccttgt tcgatgaccg cctcgactcg atttgctgtt accagccgag gactcgcaat
    13441 tacgcgtcct cgactatccg tttccaaagc tgataactcc atattaacca gcgtgccatt
    13501 attgattttt atcaaactca atcctaatgc agctggccca cccgctgctg cagccagacc
    13561 acttgcaggt aagttgacat ttaaattctg ccccccccct ggactaagca atccagatgc
    13621 caagccactg gaatgatcca aattaccgga aattccaaga ttacgattac ccatctgggt
    13681 tgcattttgc acaccaaagc gcgcgcccaa tgtgcgactg aatgtctccg tcgcttccac
    13741 aatcctagct tcaatcatca cttggcggac aggcgtatct agatttttga catgcttagc
    13801 aacttcggcc agccgaatcg gaatatccgt taccgttaaa gtattactga tatcatccat
    13861 ctcaacggtt ccacgctggc tcaatatccc agtcaatgga attgagcttg cagtccgata
    13921 attcaattga aatgtttcgg tctgaactgg ttctaactct gaaatctgct ggcgagattc
    13981 cagatccatt ttttcacgag cagccaattc ttctcttggt gcaacgaaaa taacgttacc
    14041 aacccttcgc ttatcgagct catgggattg taatacgata tcaagggctt gatcccaagg
    14101 tacatctttt aaccgaagcg tcagacttcc ttttactgaa tcgctcgcaa taatgttcaa
    14161 gttagtaaaa tccgcaatta cttgcagtac tgaacgaact tctacattct ggaaattcag
    14221 ggatagcttt tctcctgtat aacctccatt cataagcttc ttatgcgctg gcacatcctc
    14281 gctctcaata acaggtttca cttccagaaa aaaacgtgtt cctgactgat aggatgtatg
    14341 ctcccaacgt cctttaggtt caacaacaag ctgaacatta tcccctttgg tcagcgtctc
    14401 gataatttgt actggggtcg caaaatcaag gacatccagt cttcttgcta aattaggtgg
    14461 taaatacgtt ttaacaaact cgacaacgat tcgctcccct tgcgtgtgaa catcgaccgc
    14521 agtacctggt tctgatagta atacttcgat acgcccttca ccattagcgc cccgtctaaa
    14581 atcaatatct tgtaaacttt ttaaattatt ctcacttgaa ttaataattt ttgatagcgt
    14641 tggtgtaact gcgacactac ttaaattcac tgctttaagt atgattacaa catgattatc
    14701 ttgaaccttt gcatgatgag tcatgggacg caataaattc atgaccaagc gacttctatt
    14761 ctctgtttgc acaatattaa tgctacgcaa atcactgtta ccaacttcct gcgtattacg
    14821 tcccaatcca ttaatactat tggtaaaatc aaatacgatt cgatcaggat tagtcaatga
    14881 aaaccctact ggcggagaac tcattggctg atctagtgtg attttgaccg taatctgccc
    14941 attcgtagca ttcactacat tgattgcatt aatgctatta cgaaaactct gctctccctc
    15001 gatcgtctca gcaactgcaa cagcgttctg gtaaaaaata cttatcagca ataattgcag
    15061 tatgggcagc catttttgag aatatttttt cactgctttt gtctccatta tctctgctcc
    15121 tgggcttgta acataagtgt acttggatgt tccgtccaat cgcttgcact atcctgaata
    15181 atttctctca atttgatttc agcttcagaa attccggtaa tcaatccaaa attttggcct
    15241 aggtaattcc ctactttgac acggtgcagg gtgctatcgg gcgctttaat caatgcgtaa
    15301 atttgtcgat cacgctgcaa cgtccccacc attgctaaat tttccaaggg aaaactctcc
    15361 agcacctctt tacgacgctt gaaatcaggc tgaatcgatc cttgattttc tttgacttga
    15421 ttattcttcc tgggtgaaaa gggattcggt aactcaaagg ctgcataggt aaactgagta
    15481 atagtttgaa tttcaggcaa tggttcaacg gtcccgtgta gaccttcacc cgaatggttc
    15541 acgaattcct caagatcaga atgggtattt gcttcacatc cagtcaatac gataacaaac
    15601 ccaattaata ataaacgaat cctatcgttc atttcttgcc tccactcggg gctttacctt
    15661 gacttgcgag ctcctcatca tcaagatagc gaaacgtttt tgccgtggcg cttaatacga
    15721 gctgttgatc ttttccaggc aagatctcaa tatcatgtag cgttacaata cgggataact
    15781 gagaaacatc gctggcgaaa gcaccgatat cgtgataatt cccggtcacc cgaactgtta
    15841 ccggtaactc tgcataaaat tctttaatta attcacttgc agcaggctta aataattcaa
    15901 attgcaggcc acgccctaaa ccagctctat tgatatccac cagtaatgca tctagctcgg
    15961 atttgttagg aagttgtttg agcattgcgc ttagcgaatt ttcaatttca attaactgtt
    16021 gctgcattac cactaagttg actgcacttt ttttcttagt taaaaagttt tctttgagtg
    16081 ttttttcctc agtttctgca ttttcaagct cgacccattg ctcttcccaa ataaaaaaat
    16141 agccagcaaa tatcagtaaa cagagcaaaa taatgagtgc gcccgcttta atttttgctg
    16201 gccaatttcc cggatcattc gggtttattc gttgcaattc ttctagtagg ctcatagtgc
    16261 atgcagtgat atcaaagctg ttcagtcaag ctattaggcg actcagacaa tctgacattt
    16321 aaatcaaatt cgtttgaacg actattatta atagttgctg ccttgatttc gatgagtaat
    16381 ggcgattcaa accaaggcga tgcatccaag ttgcgcatca aggtagaaac ccacgcattg
    16441 gattgtgcat aaccggacaa atggatatgt tgttcagttt gttttaaact tttgagataa
    16501 acgccatctg gcatcagtcg tgccacttga tccaagacat gcaccacttt tgctctatcg
    16561 agctgcagtg cttcaaccac tcctttgcgg gcaatcagtt cttgcttttg ttctttaatg
    16621 cgcttaattt cctcgatctg tttatcgaga atagcaattt gattttgtaa atactgattg
    16681 cgaccgttct ggtgttcaat ctctccggca atatattcat acattcccca gacaatgaac
    16741 aaccctagcg cacaagtcat tccagctaga atggcaaact gctgctggcg agccttacgt
    16801 tttagttcac gatgaggaag tagatttatt cggatcatga tggatcaaag ctacgcattg
    16861 ctaaaccaca agcaatcaaa agcagtggtg catcttgctt taattggcgt tgtgaaactc
    16921 ggttcgatag ttccatatta aagaaagggt tcgctatttg cgtaatcgtt tgcgtacgcg
    16981 cagcgataat ggcttctaaa cctgggatcg ttgcacaccc ccccgataga aaaatacagt
    17041 ctacttgcat gtactgtgtt gacgtaaaaa agaactgaaa tgctcgcgca atttctaatg
    17101 caagcgtctc acaaaagggc tgtaatacat cctggccgta attttctggt aatgttcctt
    17161 tacgctttgc tgcttcagct tcttcaactg ataagttaaa ctgattctgt atctcttgag
    17221 tcagttgatc tccaccaaat ggttgatcac gagcgtaaac cgactcaccg tcatgaatga
    17281 tatttatccg tgtaatactc gcaccaatat cgactagtgc aatcacctgg tttttcccgc
    17341 cttctggcaa ttgatgcttc attaactcaa aagcagcttg cgctgcaaaa ggttcaacat
    17401 ccatgaccac tgcttttaaa cccgctgttt gaactgcagc aaccatatct tccacttttt
    17461 ctttacgaga agctgcaatg agtacctcta cttcttctgg atggtcaggc acaggcccca
    17521 atacttgaaa atcaagatta acttcgtcta aggagaaagg tatgtattga ttggcttctg
    17581 tctcaacctg aaaaaccatg tcctcttctc tttgacctgc tggcagcacg actttcttag
    17641 tgattactgc tgctgctggt aaagcaagag cggcctcttt aaggcgtgaa ccggttttct
    17701 tccacccctt acgaatactc tcaccgacag cttcaagatt ttgaatattg ccatcaatga
    17761 ttgcttcttt ggacatcggt tcaatgacat agcgttccaa acgatacgta ttggttgatt
    17821 ttttcttgat cttggaaagt tctattatct tgactgatga tgagctaata tcaatcccta
    17881 acaatctggg cgattgagga aaaaaaagat taagatcaag attaaaagtt tttccgagca
    17941 ttgatttatt tgaaacaata ttttagagtt acagttgaaa ggcagctatt tctaagaaat
    18001 gggatcattg ctcatcatta atttcattta gcttacctat cgtagcttca cctaacttgc
    18061 tgagcattct gacagatttg atcagtattt aatttgatta aaaacagtag taataccctc
    18121 taaattggaa atcgattcct ccctttatta agcaatccct ttcatgtcac tccgttggtt
    18181 aatctatccc tttattgcgc ttttcgccgt cagtttgatt ggcattttat tagttgtatt
    18241 tgctgcactc gtcatcatcc cgaccttacc ttctcttgaa gtcatcactg attatcgacc
    18301 gaaaataccg ctacgcattt atagtgcaga gggattgctt attggagaat ttggagaaga
    18361 aagacgagat ttcgtcaata ttcgtgaaac accggtattt ctaaaaaaag cgattttagc
    18421 tgcagaggat gatcgctttt atcaacacgg aggggtcgat tatattggcg tactgagagc
    18481 tgcatactcc aatttttctg gtgggggcac attacagggt gcgagtacta tcaccatgca
    18541 agttgctcgg aatttttttc taactaaaga aaaaacccta actcgaaaat ttagtgaagc
    18601 gctccttgcc tttaagattg agcacagcct tgataaagac aagatactag aactttatat
    18661 caatcaaata tacttagggc aaagaagcca cggcttttca gcagcggcac aaacctattt
    18721 tgggaagcca cttatcaata tcaatcttgc agaagccgca atgctagcag ggttgcctaa
    18781 agcgccttca cgattaaatc cggtcgttga cctcaaacga tcaaaggcac ggcaacacta
    18841 cgtgctaaaa agaatgcatg agcttagcta tatttcagcc aaagaatacg aagaagctga
    18901 aaaacaaact attttaattc accgacaaac tcgtattaat gccatgcctg ccgatcacat
    18961 cgtcgaaatg gcacgccaag cattgtatca acagtatgga gaggatgtgt atacccgtgg
    19021 actaaaggtc tacactacca ttcacaaggc tgatcaagtg gcagcttatg aggctgtccg
    19081 taaaggaatt ttagattacg actcacgtta tggttataga ggtgcagaag cattggtaga
    19141 tattccaccc agtgatgtta atccggcaaa aacgcttata tccgcgctac aagaacatag
    19201 cgatagtgat gatctactaa tcgcagcagt aatatcttca aattccaatc agattgaagc
    19261 aattctaaag aacggacaac ccattcaaat ttcgggtgat ggcttaaaaa tagccaagaa
    19321 ataccttgct actgatccac aattagggaa taagagaatt cgcaagggtt cactcattcg
    19381 cgttcagaaa aacaatcaga accaatggaa aattacgcag ttacctgagg ttgaggctgc
    19441 gctgatttca attgatgcaa acgatggaaa aatacgtgcg ctggttggtg gttttgattt
    19501 ctacttaaat caattcaatc atgttactca ggcatggaga cagcctggat caagctttaa
    19561 acccttcatc tattcagcat ctctagaaaa aggatttaca cctgccacca ttattaatga
    19621 tgctcctatt tattttgatg ccgctcaaac agggagtaaa gcatgggaac ccaagaattt
    19681 cgatggtaag tttggtggcc cgattcgcat gcgtgttgcg cttgcgcaat ccaaaaatct
    19741 tgcatcgatt cgaattctac aggccattgg cccacgctat gcacaagact atattgcacg
    19801 ttttggcttc gatagcaacc gccatccacc ctatttaccc atggcattag gggcaggctc
    19861 cgtaactcct ctacaaatgg catcgggtta cgccatattt gcgaatggtg gatataaggt
    19921 agagccttat ttcattgagc gcgttgaaga cgataaaggc aatatcgttc agcaagtcga
    19981 tccacttgtg gcaggtcaaa atgcaacaca aattatcgac ccacgcaatg cgtttctgat
    20041 gaacagtatg atgcaagatg tcatacaacg gggtactgcg acagcagcaa agcaacttaa
    20101 acgtaatgat ctcgctggaa aaactggaac taccagtaat ttcattgatg catggttttg
    20161 cggataccag aaagacttga ttaccgtttc ctgggtggga tttgacgaac cgaagtcact
    20221 tggaaaaaat gaaaccgggg gtaaggtagc gttaccgatc tggattaatt atatggagaa
    20281 agcgttaaaa cagatcccag ttgcgaccta tacccctcca aaaggaattg ttaccgcaaa
    20341 aataaatcca gctactgggt taagagaaac agcaggagga atgagcgaat acttttttca
    20401 agagcacctt cctcctgtgg caacagttaa tgatagtgat gaagaccttt tatcaaatga
    20461 cgatttctgg agtcaagcct tttagattct cctattcaat tgcttcctac gacccgcttt
    20521 gttttaacca accggctgcg tctaatgcaa aataggtcat aatcgcatca gcaccagctc
    20581 gtttaaaagc aagcagcgcc tctagaacac aagcttcctc atttagccaa ccattgattg
    20641 ccgctgcttt gagcattgca tactcaccac tcacttgata aacaacggtg ggagcaccaa
    20701 atttctcttt cactctgcga acaatatcca gatagggcat acctggttta atcatcacca
    20761 tatctgcacc ctcttgtaaa tctagcccaa cttcccataa tgcttcatct gaattggcag
    20821 gatccatttg ataagtgtat ttattacctg atttcaagtt cgctgacgaa cccaccgcat
    20881 ccctgaatgg cccataaaaa cttgacgcat atttcgccga ataggcaaga atccgcgtat
    20941 ggatgtgctg attatcatct aatttttttc gaattgcagc caccctaccg tccatcatat
    21001 cagaaggtgc aacgatatca gccccggctt gggcatgtac tacggcttgc tgctctaaca
    21061 ctaaaattgt ctcatcattt agtacataac cattctcatc aatcaatcca tcctgtccat
    21121 gactcgtata agggtccagt gcgatatcag taatcacacc taattctgga aaatgctttt
    21181 ttaatgctct caccgtccta ggtactaacc cctcagggtt tagtgcttca tgagcagtta
    21241 agctctttaa tgaggcgtca acaaccggaa ataacgccat tgctggaata cctaaattca
    21301 cacattgctc agcttgatgc aatagcagat cgatacttag ccttgagaca cccggcattg
    21361 aagcaactga ttctttgaga tgttgtccat ctaacacaaa tacagggtaa ataagatcat
    21421 caacacttaa taaattctct ctaactaaat ttcttgaaaa gttatcacga cgtaatcgcc
    21481 gcattctttt ttgtgggtat tgacttaatg gtgacatagc aatttaagaa aaaaattgaa
    21541 ataaaatgaa cttaacagag aatttcgtct catatgctta gacgcaagca tatgatgatc
    21601 gtctctcgcc ttccctccct gaggcgaggc ctttgagata aggcaactcc cccggttttt
    21661 cccctttgac cgggggtttt ttttgcctaa tgtgacagta aagcatcata ctgtaaaatt
    21721 agtcggttga tttaaaccaa tttgataata cctgcaatgc ttcctcgacc ccgacagttg
    21781 ctaaattaga gaataattgg gctgtgcaat gcggatattg ttctaacaaa aaattttgta
    21841 cgctttttaa ggttttcatt gattggtttc gtgacaactt atctgactta gacaacaaaa
    21901 tgtgaaccgg tttatttgtg atcgaaaacc actcaagcat cttttgatca agctcagtca
    21961 gtggatggcg cacatccata atcaatatca acccaactaa attagaccgg ctttgtaaat
    22021 aagtactcag taaatgcctc caatgctcac gtatcaattg cggtactttt gcgtatccat
    22081 aacctggaag atcaaccaga aaattaccat cattcaattg aaagtaattg atatgctggg
    22141 tgcgcccagg cattttactg acaaaagcaa gtcggttacg cctcgtcaat gtgttaatcg
    22201 cacttgattt tcccgcattt gagcgaccag caaatgccac ttctatgttt gttgtttttg
    22261 gaagatcctt aaggtgattg accgtcgtat aaaatcttat cccttgtaaa attgacatca
    22321 tctgatcatc gctcaagtaa atatagcact cgaatctgtc aatgaaataa tccttaaccc
    22381 ttctagcact agatcatcta cccattcttt gccagcaata tcgatataag gcaacaccgt
    22441 ttgagcacct ttcccgctaa taatacattt aggattagaa tgacttaagc tctttgtaaa
    22501 tatgctatac atctgctcga ttgcgccagt taacccataa attgcgccgc tcaccatggc
    22561 atcttgggta ttacgcggta ccttttgaaa acaaccgttg ctggaatcgg ttaataacgc
    22621 agtttggttt attaacgtgc tttgcatcaa atcaaatcca ggcaaaataa ttccacccat
    22681 aaattcacct tgattagaaa gaatatcaac cgtcatggtg gttccaacac tcacgactaa
    22741 acaggcttgt tgcttcatgc tccatgcagc aattaaagca gcccatcgat cacttcctag
    22801 ttgagaagga atgtcataat aattcttaac cccacattga tcactttttg agaaagccca
    22861 atttaccttt gctgtccact gaagctgttt aaatatcagc atcagtgatt gttctgcgag
    22921 tgatccagcc acattggaga tcgtaatttg tgatggttcc ggtattttcc cccagacacc
    22981 tcccagtgaa tcgcgatgcg tctgcatggc aaccccttta tcaatccatt tatccttatc
    23041 cgccaatccc cactttatgg cagtattacc tgaatcgata acgagcaaat aagtcattga
    23101 ataaaaaatt ctcagccaat tttacgcaag ctaatttcac cacaatgaaa gcttttcaat
    23161 ccagattcca tttgtagtcg taacgaacca tcttccgtta ttccagttac aattcctctt
    23221 tcaatcaacc ctgctggcaa taacacttca acttcccttg cttcatagct atgataactg
    23281 atccactctt ccttaaaagg tgagaagcct tcagccgcaa aaatttctaa taccgttgcc
    23341 atttcaatca gcaatgaagc aagtagttta ttgcgatcaa ttggggtatg cgttatcgaa
    23401 taaagatctg agatatcctg atcaacgtaa agcttagttt tatccgataa ggttagattg
    23461 attccaatgc caattactgc tgtagctggc ccctgtactt ctccctgtaa ttcaatcaat
    23521 accccaccta atttactgct attgaataat aaatcattag gccactttag ggcgacctct
    23581 cttattccga gtgacgtaaa ggttcgtatt attgcaattc caactactaa gcttagacca
    23641 gccagaaaat tgactccttg ctgaaattgc caagataaag aaaaagttaa actatcacct
    23701 aaaccagaaa accacgagcg ccctcgacga cccctgcctt ttgtttgtaa ttccgttacc
    23761 accacatcaa tcatcgattg atcataattt tttgctttac ttgtatttaa cagaattgta
    23821 ttagtggatt caacgacttc atcaatcgtt attcggaatc gctgtgaata ttcagcaaga
    23881 tattgattaa tagcattctt atccaaccat tgcaccggat tgatccagcg atatcctttc
    23941 ccggttacac gatagagcgt cacaccaaat tcatcgatac cacggagtgc attcgataca
    24001 ctggtacgag aaacgcccaa aaattgggca atttgcgttc ctgaatgaaa ctggccatcg
    24061 ctcaataact ttaaaatcgc aaaagagatt ggtttcaccg agaatctaag ctttaaagaa
    24121 tagatttaag gtattgctat gaatcatggc aatacaaacg agaacttgag caagctaggt
    24181 atttataaac tatcttgata actaagttag ccaaaaatag agcgcgatag agaaaatttc
    24241 tatctgaata gaatttacct ggcgtcccca aggggattcg aacccctgta ctcaccgtga
    24301 aagggtgatg tcctaggcct ctagacgatg gggacaagat cataaaaagt attgcaaaaa
    24361 ctggtggaga taagcgggat cgaaccgctg acctcttgca tgccatgcaa
//
LOCUS       DAOQKQ010000012        24226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_867_length_24226_cov_6.267842, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000012 DAOQKQ010000000
VERSION     DAOQKQ010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 24226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24226
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_867_length_24226_cov_6.267842"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="PKZ49_01545"
     CDS             complement(<1..552)
                     /locus_tag="PKZ49_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HND35275.1"
                     /translation="MLRLFILMGLFGLASSVGWAQTLANQYTVLEIQQIGLEANGLVQ
                     AARSQVNIAKAGVIAARAYPNPEVTVMAGPDHGRYPGKDTEGPFSMQRSVTVNQAIEN
                     PFMRSSRIGAAEAFVEAGRASFDQARTDLAAQLRVRTYELLLRQEQAALEQNIYSLIE
                     DVRRRIKHNVERGEAARFELVRAD"
     gene            763..1833
                     /locus_tag="PKZ49_01550"
     CDS             763..1833
                     /locus_tag="PKZ49_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HND35276.1"
                     /translation="MINSSLLTYKPSMHIHLLIPNLFWSDTSLKETIKNWSLPALETI
                     LTKSQLNQQPSEGIEAWLCETFGISKQQDWPIAPITLNLDDPNYSLKSSSKYWLRADP
                     VHLRIANNHLLLADSQMFKISLTEASQITDTFNRYFSKDEISFAAFYPDRWYMGLPRI
                     PNLQTRPLSYVSGKNINAQLPMTTEKGLAHKILNEVQMLLHEHPINLAREIQGELIIN
                     SVWLWGGGILPKQFCCNFDQVWSNYPITQALANLCNAPHYPLPPDTNEWLQSTNEGNH
                     LVVIDLLQKKAQYKIINEWYDCINQLEQKWFSPLLNGIKKDQIKQLTIHTFNQDLVKQ
                     FKLTPRGLWKFWSFNKAFSTFH"
     gene            2055..3308
                     /locus_tag="PKZ49_01555"
     CDS             2055..3308
                     /locus_tag="PKZ49_01555"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HND35277.1"
                     /translation="MIIQTLSIERNYAEYLTQLHNNKERLANEIKHRLWAEKTTSENK
                     TRLQIIDENFPVMLAYFNSKQQCCYHNRAYRLWFSLKSEQIDNHSPQQFLNTEFYQGI
                     KENIDNLLSGEIIHGQRIQKLISGSVCLISEQFIPHFDAKGKVIGFYTLYTPRILKEH
                     ESITENSKNNLVEIRSEKTGAEEKSSQNKNSRLSSEMNFASRVLKAINQGEFHLYFQS
                     IISTNQGPNQITFCEILIRMAEEETNLIPPGAFLPLVEKYNLMPQLDRWVVGYITKWL
                     SSHPTKSNTIMCINIAKDTLCDPQFTIFIKNQLTTYNIPAKTLCFEIEEVDAKSNLSE
                     VATFTQNIRQIGCYVSLCSFGHDRTSFNLLRNIKVDFIKIDGNLICDMLYNETDQTKV
                     SEINRLAHIMKIKTIAELVESQEIA"
     gene            complement(3405..3656)
                     /locus_tag="PKZ49_01560"
     CDS             complement(3405..3656)
                     /locus_tag="PKZ49_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HND35278.1"
                     /translation="MISSDQIKKSIEASIPCEWIQVEGEDGRHFNAVIVSAEFVGKTM
                     IQQHQLVYRALRERMKDDVHALSMRTLTPEQWVNAQSSK"
     gene            complement(3723..4478)
                     /locus_tag="PKZ49_01565"
     CDS             complement(3723..4478)
                     /locus_tag="PKZ49_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HND35279.1"
                     /translation="MTGFLTLFHKELLRFWKVSFQTILAPIVSTLLYLLIFSHILESR
                     VQIYPGVSYSQFLIPGLIIMAILQNAFANSSSSLIQSKISGNIIFVLLSPLSYFEFFG
                     AYLLASVVRGLLVGLGMFIVVSQFHEVSTQFPFWIILFVLLGSMILGVLGIIAGICSD
                     KFDQLAAFQNFIILPSTFLSGVFYSTLSLSPAWQKLSYVNPFFYIVDGFRYGFLGVSD
                     VSPYLSLAAVIACFLVVSLLTLNMLKTGYKFRH"
     gene            complement(4475..5389)
                     /locus_tag="PKZ49_01570"
     CDS             complement(4475..5389)
                     /locus_tag="PKZ49_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HND35280.1"
                     /translation="MTPAIEIQRLNKSFGNLVALEDIQLSIAQGEFFALLGPNGAGKT
                     TLISIVAGLVMANSGQVKVMGHNVVTSYSKARSVLGVVPQELVFDPFFTVRETLVFQS
                     GYYGIRKNKVWIDEILHRLDLADKADTNMRALSGGMKRRVLIAQALVHKPPVIILDEP
                     TAGVDVELRQALWSFVKQLNQDGHTIVLTTHYLEEAEALCQHVAMLKKGKIIVLDSIR
                     NLIDKIRDISLQLRLACEVLPMQLIKWSSIKQNECYIFKIQDYLEISEILTILKQEEI
                     EILEMKLQEPDLEEVFIRMMSDKSFIGT"
     gene            complement(5458..5742)
                     /locus_tag="PKZ49_01575"
     CDS             complement(5458..5742)
                     /locus_tag="PKZ49_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HND35281.1"
                     /translation="MAIYLDGNKVALSGSITFNNVVELTQQGIDLMDSHCQIIDLNEV
                     TEVDSSAVSMLLEWLRIAKTRKLDLHFVHLPENLSSLLKLYGLSEIILPT"
     gene            complement(5791..6408)
                     /locus_tag="PKZ49_01580"
     CDS             complement(5791..6408)
                     /locus_tag="PKZ49_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HND35282.1"
                     /translation="MKKMINTLISLMLLCVALSGWAKDTAPDLLVKNTVDEVIAIIKQ
                     DNGIKKGGKDRILDLVENKILPHFNFTRMTQLAMGKNWSKVEPQQQKELVNEFQTLLV
                     RTYSNALTNYRDEVIEVNPISPQSDAKNTTVKTVVIQGRGKEPVPIDYSMEKTDNGWK
                     VFDVTVAGVSLVTNYRSTFNSQIKKGGVNGLIESLAKKNKSLEGQ"
     gene            complement(6445..6903)
                     /gene="mlaD"
                     /locus_tag="PKZ49_01585"
     CDS             complement(6445..6903)
                     /gene="mlaD"
                     /locus_tag="PKZ49_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HND35283.1"
                     /translation="MSRTVMDFWVGLFVMAGIGALLILGLKVGNLTSYNAGEVYNLTG
                     NFENIGGLKIRAPVKSAGVVVGRVLDIQYSLETHDAIVTMGVSSSYQFPKDTFASILT
                     SGLLGEQYIGLSPGGDAVMLADGDKIMKTNSAMVLEEMIGRFLFDKASEN"
     gene            complement(6903..7700)
                     /gene="mlaE"
                     /locus_tag="PKZ49_01590"
     CDS             complement(6903..7700)
                     /gene="mlaE"
                     /locus_tag="PKZ49_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HND35284.1"
                     /translation="MRNRIVVSIQAIGHRTIDSIWRLGVASRFFVWVLLKSTTSFLRF
                     GLVVREIYFVGVLSLIIILVSGLFVGMVLGLQGYETLQKYGSESAVGTLVALSLVREL
                     GPVMAALLFASRAGSAITAEIGLMKATNQLAAMGMMAVDPIARVVAPRFWAGVISMPF
                     LAAMFSVMGVFGGYLVTVVFIGVDNGSFWSQMQNAVDFRYDIVNGVIKSCFFGVAVTA
                     IAVFEGFDAPPTAEGVSGATTRTVVTSSLVILGLDFVLTSFMFRGVS"
     gene            complement(7693..8502)
                     /locus_tag="PKZ49_01595"
     CDS             complement(7693..8502)
                     /locus_tag="PKZ49_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HND35285.1"
                     /translation="MTDNLIEIKNLAFSYGTRPILNNVNMAMSRGKVIAVMGGSGSGK
                     TTLLRLIGGEIKPSGGHVKVADQIVHHLSRDELFKLRRKMGMLFQFGALFTDISVFDN
                     VAFQIREHTTLPESMVRDLVLMKLQAVGLRGAHHLMPAELSGGMARRVALARSIALDP
                     MLVMYDEPFTGLDPISLSVIGNLIRRLTDALGMISIVVTHDVQESLKIVDYVYFLADG
                     VIAAQGTPQEVKNSVAPFVHQFVHGEEDGPVPFHYPAQSYQKDLALENFVA"
     gene            8611..9501
                     /locus_tag="PKZ49_01600"
     CDS             8611..9501
                     /locus_tag="PKZ49_01600"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HND35286.1"
                     /translation="MKLILINHQLPAMRLYSQPLSFINLILIILVISATPFSAQGNTI
                     YKSTDAAGRVTYSDTPTPDARPIHLPSTPALTLHQVTTVLDGDTIVLKGNQHIRLLGI
                     NTPETNTRYREAEAGGIVAKKWLQEKLTGVKVYLEHDQLKTDHYNRSLAHVRTKNGEH
                     INLILVEKGLAFVNLIPPNLRHADALISAQRKAEKEKLGIWAYPDYAPFPITQIANKK
                     TRSWQRFYGKPISIKRSKKYSRLFFSSNIDVRFENKYLTLFPSLETYLNKTIEVRGWV
                     SRRNDHYSISIQHPSAIVIR"
     gene            9638..10045
                     /locus_tag="PKZ49_01605"
     CDS             9638..10045
                     /locus_tag="PKZ49_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HND35287.1"
                     /translation="MATIQSKIVADLETLYLENSSKNCPEEHVYEWLILGITIDGRTF
                     RPSDWAERLCGAIAQYNNGRLIYSELAYPISRNGQIGIVIETLLRSENPTIYSFFMDF
                     ARDNRLKIISGREAPRSLNTNSTQNNRPLRLVI"
     gene            complement(10105..11013)
                     /gene="ftsX"
                     /locus_tag="PKZ49_01610"
     CDS             complement(10105..11013)
                     /gene="ftsX"
                     /locus_tag="PKZ49_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HND35288.1"
                     /translation="MRIWISQHWQALGLTAKQFFFKPVSNFLSVAVIGIAFSFPLGIY
                     TLLDNLQSFSDHFVHSPQISLFLEIDATESDIENINSRLESFDGITSFQFVSNEIALA
                     QLQHESGMGDIALHLKKNPLPHAFIIHTQHMPPDKLEENRLTLQQWPAVSYVQVDSDW
                     TRKVHAFVKLGRVAVIMLSSLLGVALVIVIFNTIRLQILTKQEEIEVSKLIGATDRFI
                     RRPFLYFGALQGVVGALIAWIIITVGAQKINDNLTELSQLYELSLRLNPLSLFQVLLL
                     GAVAGFLGWLGARLSVAQHLSQIEPK"
     gene            complement(11010..11663)
                     /gene="ftsE"
                     /locus_tag="PKZ49_01615"
     CDS             complement(11010..11663)
                     /gene="ftsE"
                     /locus_tag="PKZ49_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HND35289.1"
                     /translation="MITFDKVYKRYPDGYEALTNLIFTIDTGEMVFITGHSGAGKSTL
                     LKLIAAIEKPTSGSITVSDQIVNKLKKSMVPFLRRSLGIIFQDQKILYDRTVFANVML
                     PLQIHGFEKSVMERRVRAALDKVGLLNKEKAYPITLSGGEKQRLCIARAVVHRPLILI
                     ADEPTANLDVAYAQNIMDVFRSFHQVGVTVLIATHDQTILNDSTARILSLNQGHLMV"
     gene            complement(11676..12659)
                     /gene="ftsY"
                     /locus_tag="PKZ49_01620"
     CDS             complement(11676..12659)
                     /gene="ftsY"
                     /locus_tag="PKZ49_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HND35290.1"
                     /translation="MFSFFKSQKNPPEDQSQSVSFVSKIKQGLARTRENLGKQLSSLF
                     GARKIDEAVYEELETILLTADVGIQATNQLLDSLRGKVKRDQLSDTHQLKGVLQETLI
                     ELLQPLARPLDISTHQPFVIMIAGVNGVGKTTTIGKLAYYFQSQGKSVLLAAGDTFRA
                     AAREQLVEWGKRNNVTVIAQESDSPGKSDPAAVIFDAINAAKARNIDIVLADTAGRLT
                     TQMHLMEEIKKIKRVINKALPDAPHEVLLILDANTGQNAISQVKTFNEALNVTGLVLT
                     KLDGTAKGGVIAAIAAQYPNNPPALRFIGIGESLDDLRPFDAIEFVEALFD"
     gene            12818..13897
                     /gene="alr"
                     /locus_tag="PKZ49_01625"
     CDS             12818..13897
                     /gene="alr"
                     /locus_tag="PKZ49_01625"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HND35291.1"
                     /translation="MSRPISAQINLAALRHNLSIVRQYAKHSRIMAVIKANAYGHGLI
                     RVAKTLHEIDAFALLELEAAIQLREIGIHQPILLLEGFFSEADLISIDHYQLDTVIHH
                     PEQIAMLAKHRANKKINLFLKINTGMNRLGLAPREGTKAIHTLKNLSHVGEITLMTHL
                     ACADDPAADREINDQLNIFSEILNITRLPCSLANSAAIIQHPQTHQDWVRPGIMLYGA
                     SPLIHKTATELDLKPVMTVSSKIIALQQLNTNDKVGYGGTFRAQQPMQIGIVAAGYAD
                     GYPRHAQTGTPIIVNGQRTRIVGRISMDMLTVDLTGITNVTVGSPVILWGNHLPIEEV
                     AKSANTISYELFCSLSSRVKVITTE"
     gene            complement(13947..14735)
                     /locus_tag="PKZ49_01630"
     CDS             complement(13947..14735)
                     /locus_tag="PKZ49_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HND35292.1"
                     /translation="MYLRKFLLLMLVMITGLVITSSLFAQTVAKVNGVGIPQARLDFM
                     VKAAIAQGQQDSTELRNSLRENLIAEEIVAQEAIKSGLDKSSDVMTQIELARQAILVR
                     AYQAEYIKNNAVGDDVLRREYEEVKSQMGDKEYKARHILVETEKEAKDIIAALKKGGA
                     FDKLAKEKSIDTGSKSNGGELGWSSAGAYVKPFADALQNLKKGNTTDQPVQTSFGWHV
                     VRLEDVRPAVAPPFDEVKMNMQQRVLQRNFASTIQNLRNSAKVE"
     gene            complement(14873..15175)
                     /locus_tag="PKZ49_01635"
     CDS             complement(14873..15175)
                     /locus_tag="PKZ49_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HND35293.1"
                     /translation="MLYVINGEDVPNSSEKRLIARPGHLERLQVLQQEGRLVLAGPYP
                     AIDNVDPGPAGFSGSLIVAEFESLEVAQAWANADPYVLAGVYLHVTVKPFKKVFPT"
     gene            complement(15213..15527)
                     /locus_tag="PKZ49_01640"
     CDS             complement(15213..15527)
                     /locus_tag="PKZ49_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HND35294.1"
                     /translation="MAFLSEKNIEVEIAYALSRHQFLQRITVPSGITVEQAITLSEFD
                     KRFPDIDWVSQKIGIFGKLTTLDAVLQNNDRVEIYRPLQCDPKENRRIRYKKKTVRLS
                     GI"
     gene            complement(15511..15951)
                     /locus_tag="PKZ49_01645"
     CDS             complement(15511..15951)
                     /locus_tag="PKZ49_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HND35295.1"
                     /translation="MAEIKKVVYVDYSAGQMFRLVDTVENYPEFLPWCDGTSVKQLSD
                     TIVHATVNINYGPVRHSFTTENTRQVPELIKMKLLDGPFEELDGEWRFISLSEHECKI
                     EFNLHYTFSNRLLEKLVGPVFFGIANSFVDAFIKRAEKIYGISQ"
     gene            16049..16492
                     /gene="smpB"
                     /locus_tag="PKZ49_01650"
     CDS             16049..16492
                     /gene="smpB"
                     /locus_tag="PKZ49_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HND35296.1"
                     /translation="MSIAQNKKAFHDYFIEEKYEVGIVLEGWEVKSIRAGRVQLKEAY
                     VIIRNNELYLIGSHISALPTASTHINPDPLRTRKLLLHAEQIKRLIGKVERAGYTLVP
                     LDMHYKTGRIKLEIGLAKGKKQYDKRESEKQKEWDRTKQRLLRAR"
     gene            16529..17554
                     /locus_tag="PKZ49_01655"
     CDS             16529..17554
                     /locus_tag="PKZ49_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HND35297.1"
                     /translation="MQKPIAIWLFICCALVFAMVVVGGVTRLTGSGLSIVEWQPLIGA
                     IPPLSEADWEILLEKYRQIPQYHEVNKGMTLEEFKGIFWWEYFHRLLGRAIGLVFFIP
                     FLYFLIRKQIDRPLGLKLLGIFVLGGLQGLMGWYMVMSGLVNNIYVSQYRLTAHLGLA
                     FIIYAAMFWVATGLLSPAKKQQQPETESYYSHLRRYSFYLTLLIFIMILSGGFVAGIH
                     AGKAYNTFPLMDGHLIPPGLFVLEPWYRNFFENITTVQFDHRLIAWLLAFFVPFFWLR
                     CIKYSLSPSAKMACHLLLLALILQISLGVATLLSAVPLTLAATHQAGAMVLFTASLWV
                     NRKLSSI"
     gene            complement(17897..19561)
                     /gene="ettA"
                     /locus_tag="PKZ49_01660"
     CDS             complement(17897..19561)
                     /gene="ettA"
                     /locus_tag="PKZ49_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HND35298.1"
                     /translation="MAQYVLTMNRVGKIVPPKRTILKDISLSFFPGAKIGLLGLNGSG
                     KSTLLKIMAGVDKDIEGEVTPMPNLKIGYLPQEPQLNPDQTVREAVQEGLGDIASAQQ
                     KLDEIYAAYADPDADFDALAAEQTRLEAIIATGGGDTAQQMEVAADALRLPEWDAVIR
                     NLSGGEKRRVALCRLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAVTHDR
                     YFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEIRLKQEESSESARQKALQKELEWIR
                     QNPKGRQAKSKARIARFEELNSLEYQKRNETQEIFIPVADRLGNEVIELMDVSKAFGD
                     RLLIDNLSFKIPPGAIVGIIGPNGAGKSTLFRMIMGKEKPDSGEIKIGETVKVAYVDQ
                     SRDVLESDKTVFEAISGGNDILVVGKYETPSRAYIGRFNFKGPDQQKIVGQLSGGERG
                     RLHLAKTLISGGNVLLLDEPSNDLDVETLRALENALLEFAGCVLVISHDRWFLDRIAT
                     HILAFEGDSQVTFFAGNYQEYESDKRQRLGGEGAKPKRIRYKPIAR"
     gene            19755..19934
                     /locus_tag="PKZ49_01665"
     CDS             19755..19934
                     /locus_tag="PKZ49_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35299.1"
                     /translation="MKVMVIFKTGTSQVFIVPRDILAVEFRRLAESVGGEIHRIEFMQ
                     KNKFAAPKYALIKDI"
     gene            complement(19947..21146)
                     /locus_tag="PKZ49_01670"
     CDS             complement(19947..21146)
                     /locus_tag="PKZ49_01670"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HND35300.1"
                     /translation="MTKQTQDAYIVAAVRTPVGKAPRGMFKNVRPDDMLVHVLQAVMK
                     QCTGLDPAAIDDVVAGCAMPEAEQGINVARVASLLAGLPDSVPGMTVNRFCASGLQAV
                     AIAADRIRLGEADVIIAAGTESMSMVPMMGNKVAMNPAMFTHNEHVAIAYGMGMTAEK
                     VAQQWKITRDQQDEFALTSHTRAINAIDMGEFDDEIAPYAIEENRPDLSSHEVITVKT
                     MRDTDEGPRADTSLDALAKLKSVFAADGTVTAGNSSQMSDGAGAVVVMSAAALKRFSL
                     EPIGRFVGYAVAGVPPEIMGIGPIKAIPKVLKQTGLKQEELDWIELNEAFAAQSLAVI
                     RDLGLDRNKVNPLGGAIALGHPLGATGAIRVATLLHGLRRHKQKYGMVTMCIGSGMGA
                     AGVFESL"
     gene            complement(21190..23685)
                     /locus_tag="PKZ49_01675"
     CDS             complement(21190..23685)
                     /locus_tag="PKZ49_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HND35301.1"
                     /translation="MSDHRFMVRKAAVLGAGVMGAQIAAHLVNANIETLLFELPAEAE
                     NPNANVTKAIERLKKLEPSPLSVISKSTFIHPANYDQHLGSLTDCDLVIEAIAERVDW
                     KTELYAKIIPYLAPHTLVASNTSGLSINQLAAALPEELQHRFCGIHFFNPPRYMHLIE
                     LIPCTYSDQTTLDHLESFLTTYLGKGVIRAKDTPNFIANRVGVFSMLATIHHASAMGL
                     SFDLVDQLTGTLIGRPKSATFRTADVVGLDVLSHVVETMRHSLAQDPWHSYYALPGWL
                     QQLITQGALGQKSSRGVYQKKNKDILVFNPAKNEYENSTATIDEDIQQLLKQKDPARK
                     FSELRENPHPQAQFLWAIHRDLFHYCAVHLAEIANNARDLDLAVRWGFGWTLGPFEIW
                     QAAGWVPITKWINEDIAAGRAMSQTPLPDWVMELGKTAPPGVHGTMGSFAPLTKGYQP
                     RSTLSVYRRQPFPDRLIGEEHRYGETIFENNAVRMWHTGDDIAIVSFKSKMNTIDIEV
                     LDGLQQAIEEAEHHWSALVIWQTEPPFSAGANLQKATEKPKNDEPPSPFDQFVKKVKR
                     TAQSTILQAARSLDLADVLMASKLAEVETLITRFQQLSQHLRYSMIPTVAAVDGLALG
                     GGCEFVMHCDRAVATLESYIGLVEAGVGLLPAGGGCKELALRAAKESVDGDPFPKLKH
                     YFQTVAMAQLGKSAEQAKELGYLRQADMIVMNRFELLHVAKTQANAMAEAGYRPPLQF
                     RDIPVAGSTGVATIKGMLVNMLEGHFISEHDYLIGSKIAHVMCGGNLTPGSLVDEEWF
                     LRLERTAFIELLATEKTQARIAHTLKTGKPLRN"
     gene            24046..24122
                     /locus_tag="PKZ49_01680"
     tRNA            24046..24122
                     /locus_tag="PKZ49_01680"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24080..24082,aa:Pro,seq:tgg)
     gene            24150..24226
                     /locus_tag="PKZ49_01685"
     tRNA            24150..24226
                     /locus_tag="PKZ49_01685"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24184..24186,aa:Arg,seq:tct)
BASE COUNT         7432 a         5519 c         4237 g         7038 t
ORIGIN      
        1 atctgctcga actagctcaa aacgagcagc ttctcctctt tccacattat gtttgatgcg
       61 tcgacgcaca tcctcgatca aactatagat gttttgctcc aacgctgctt gttcctgccg
      121 aagcaatagt tcataggttc taactctaag ttgtgctgcc agatcggttc tagcttgatc
      181 aaagctggca cgccccgctt caacaaaggc ttctgctgca ccaatccgag aactccgcat
      241 aaatggattc tcaattgctt gattaactgt cactgaacgt tgcattgaga acggcccttc
      301 tgtatctttt cctggatacc ttccgtgatc tggaccagcc atgaccgtta cttctggatt
      361 gggataggcc cttgctgcta taacgcctgc tttagcgata ttaacttgag aacgagctgc
      421 ttgcaccagc ccatttgctt ccaacccaat ctgctgaatt tctagcacag tatattgatt
      481 tgccaaagtt tgcgcccaac cgactgagct cgctaaccca aacaaaccca ttaagataaa
      541 taatctcagc atggtaatct ttaataaata attaagaatt tgcgtaaaat tctagaattt
      601 tacatgaagt atttgtgatc aagcatttaa gttgcaggct aaatacaaag tatttagtca
      661 atctagccaa ggtaaagtat aaactgtgat aaatgaatac aagctgaacg atttattcta
      721 aagaagctat ttctagattc tctgcctaat ctgttagatt agatgattaa ctcatcatta
      781 ttgacttata aaccttcgat gcatatccac cttttgattc ctaacttgtt ttggtcagac
      841 accagtttaa aagaaactat caagaattgg tcgctacctg ccttagaaac aattttgaca
      901 aagagccaat taaatcaaca gccatcggaa ggaatcgaag catggttatg cgaaacattt
      961 ggtatatcaa aacagcaaga ttggcccata gcgcccatta ccttaaactt agatgatccc
     1021 aattacagcc taaaatcgag ttctaaatat tggctgcgcg ctgatcctgt gcatctcaga
     1081 attgcaaata accatctgct cttggcggat agtcaaatgt tcaaaatatc tttaacggaa
     1141 gcaagccaaa ttaccgatac attcaaccga tatttctcta aagatgaaat ctcttttgcc
     1201 gctttttatc cagatcgatg gtacatgggc ttacccagaa ttcccaatct tcaaacaaga
     1261 ccgctcagct atgtaagcgg gaaaaatatc aatgcgcagc ttccaatgac aacagaaaaa
     1321 gggctagcac acaaaatttt aaatgaagtt caaatgctcc ttcacgagca tcccattaat
     1381 ctagcaagag aaattcaagg tgagttaatt atcaatagcg tttggttatg gggaggtgga
     1441 atattaccta aacaattttg ctgtaacttt gatcaggttt ggagtaatta ccctataact
     1501 caagcgctag cgaatttgtg caatgcgccg cattacccgc tcccgccaga tacaaacgaa
     1561 tggttgcagt ctactaatga aggtaaccat ctagtagtaa ttgatctttt acagaagaag
     1621 gcgcaataca aaattattaa tgaatggtat gactgcataa atcaattaga acaaaaatgg
     1681 ttctcccctt tattgaacgg aataaaaaaa gatcagatta aacaacttac gattcatacg
     1741 tttaatcaag atttagtaaa acaattcaag ctaaccccta ggggtctttg gaaattttgg
     1801 tctttcaata aagcattctc aacatttcat taatttagat cctagttatt aatttataaa
     1861 atactcatta aataaccgac cgatcaatga acgaacaact atctcccaga attagccaca
     1921 caaagtccta tgttgtttca tctattgcta tcattgtttt agcaatactc atggcatcaa
     1981 actttctgac tctgggactt gaatcaaatg taatcagtat cttgtggggc acccttgtca
     2041 ttgtcgtgat aacaatgatt atacaaacgc tcagcattga acgaaactac gcagaatatt
     2101 taacccagtt gcataataat aaggaaaggc ttgcaaatga aattaagcat cgtttgtggg
     2161 ctgaaaaaac tacatccgaa aataaaactc ggctccaaat tatcgatgaa aactttccag
     2221 tcatgcttgc ttacttcaac tccaaacagc agtgctgtta tcacaacaga gcctatcgat
     2281 tatggtttag cttaaaatct gagcaaatcg ataatcattc cccacagcaa tttctaaaca
     2341 cagagtttta tcaggggata aaagaaaata ttgataactt attaagtggt gaaatcattc
     2401 atggccagcg tatacagaaa cttattagtg gctcagtttg ccttatctca gaacaattca
     2461 ttccccattt tgacgctaaa ggaaaagtta ttggctttta tacactgtat acgcctagaa
     2521 tactaaaaga acatgagtca attacagaaa attctaaaaa taacctagtt gaaattagat
     2581 cagaaaaaac cggcgccgag gaaaaatcaa gtcagaataa aaattctcgc ctttcctcag
     2641 aaatgaattt tgcttccaga gtactaaagg ccattaatca aggtgaattt catttatatt
     2701 tccagagtat tatctctacg aatcaaggtc ctaatcagat tactttttgc gaaattttaa
     2761 ttcgaatggc cgaggaagaa acaaatttaa ttccaccagg cgcattttta cctttagtag
     2821 aaaaatataa tttaatgcca caacttgatc gctgggttgt tggctacata acaaaatggc
     2881 tttcttcaca tcccacgaag tctaatacta tcatgtgcat taacatagcc aaagataccc
     2941 tttgcgatcc ccaattcact atctttatta aaaaccaatt aacaacatac aatattcctg
     3001 caaaaacact atgttttgaa atcgaagaag tcgatgcaaa atccaatctt tcagaagtcg
     3061 ctacctttac acaaaatatt cgacaaatag gttgctatgt ttcactctgt agttttgggc
     3121 acgatcgaac ttcttttaat ctgcttagaa atattaaagt tgatttcata aaaattgatg
     3181 gaaatctaat ctgtgacatg ctttacaatg aaactgatca aactaaagtt agcgaaatta
     3241 atcgacttgc tcacattatg aaaattaaga ctatcgctga gcttgtagaa tctcaagaaa
     3301 tagcttaaag cttcgtgaaa tcggggttga ttttgtgcaa ggctttggaa ttgcgcaacc
     3361 acaaccgctc aatgcgctca cagaaacatc agattagtat tcatctattt acttgattgt
     3421 gcattaaccc attgctctgg tgtaagtgtt ctcatcgata gcgcatgaac atcgtctttc
     3481 atccgctcac gcaatgcacg atataccaat tgatgttgct gaatcattgt tttaccaaca
     3541 aactctgcac tgacaatcac ggcattaaag tgacgaccat cttctccttc tacctgaatc
     3601 cactcacagg gtatggatgc ttcaatagat tttttaattt gatcggaaga aatcataatt
     3661 acctcagtag tatgtaaaat taataaaagt gcataaaaat ttaatcatcg ttaccagaat
     3721 aactagtgcc tgaatttata acctgtctta agcatgttca gtgttagcaa tgaaacaacc
     3781 aaaaaacaag caattacagc agccaaactc agatagggcg acacatcaga aacacctaga
     3841 aatccatagc gaaaaccgtc aacgatataa aaaaaaggat ttacataaga aagcttttgc
     3901 caagcaggtg aaagagaaag agttgaatag aataccccag ataagaatgt agaaggcaaa
     3961 ataataaaat tctggaaagc agcaagctga tcaaatttat ctgaacaaat tccagcaatt
     4021 atccccagaa ctcccagaat catactcccc aataaaacaa acaatataat ccagaatgga
     4081 aattgtgttg atacttcatg gaactgggat accacaataa acatacccaa acccactagc
     4141 aaacccctca ctacagaagc taataaataa gcaccaaaaa actcaaaata agatagaggc
     4201 gacagcagca caaaaatgat attgccagaa atttttgact gaattaggct tgatgatgag
     4261 ttggcaaaag cattctgtaa aatagccata attattaacc caggaatcaa aaactgagaa
     4321 taggaaacgc ctggataaat ttggacacgt gattctaata tatgtgaaaa aatcaacaaa
     4381 taaagcaaag ttgatacgat aggcgccaaa attgtttgga aactgacctt ccagaacctc
     4441 aagagctctt tatgaaataa agtcaaaaaa cccgtcatgt ccctataaat gatttatcgc
     4501 tcatcattct tataaagact tcttctaaat caggctcctg aagcttcatt tcgagtattt
     4561 caatttcttc ctgctttaaa atggtaagaa tttctgaaat ttccaagtaa tcttgaattt
     4621 taaaaatata gcattcattt tgctttatcg atgaccattt aattagctgc attggtaata
     4681 cttcacacgc taaacgaagc tgtaacgaga tatcacggat tttatcaatc aaattacgaa
     4741 tactatccaa aacaataatt tttccttttt ttagcattgc tacatgttgg cataatgctt
     4801 cagcttcttc taagtaatga gtcgtcaaca caattgtatg tccatcttga ttcagctgtt
     4861 taacgaaaga ccataaagct tgcctaagct caacatccac tccagctgtc ggttcatcaa
     4921 gtatgattac cggaggttta tgaaccaggg cttgcgctat caataccctt cgcttcatac
     4981 ctccagaaag cgctcgcata ttagtgtctg ctttatcagc cagatcaagt cggtgcaata
     5041 tttcatcaat ccatacttta ttttttctga tgccataata tccagattgg aaaaccagtg
     5101 tctcacgcac agtaaaaaaa ggatcaaaaa ctaattcctg aggaacaacg ccaagcacac
     5161 ttcgggcttt tgaatagcta gttacaacat tgtgacccat tactttaacc tggccactat
     5221 tagccatgac caatcctgcc acaatactaa tcaatgttgt tttacccgca ccatttggcc
     5281 caagtaatgc aaaaaattcc ccttgagcga ttgaaagttg aatatcctcc agcgcaacca
     5341 aattaccaaa tgatttattc agtctctgaa tctcgattgc tggagtcatc tacaaacaaa
     5401 agaagcaaac ttactttaat taaagttaag caaaaactgt gatcgagaaa tgacttatta
     5461 ggtgggtaag attatttcac tcaaaccata caatttgagt aaactactta aattttccgg
     5521 caagtgaaca aagtgaagat caagtttccg cgtctttgct atccgtaacc attcaagtaa
     5581 catactcaca gctgacgaat caacttctgt tacttcgttc aaatcgataa tttgacaatg
     5641 gctatccatt agatcaatcc cttgctgagt taattcaacg acattattaa atgtaatcga
     5701 accgctaaga gcaaccttat ttccatctag atagattgcc atgattgatt aagttcatcc
     5761 atgttaatta aaactcaatt cgcagactta ttactgccct tctagcgatt tatttttctt
     5821 cgcaagtgat tcaattaggc cgttaacccc ccctttcttt atctgactat taaaagtact
     5881 acgataattt gtcacaagac tgactccagc gactgtcaca tcaaaaactt tccatccgtt
     5941 atctgttttt tccatactat aatcaattgg aacaggttct ttacctcgcc cttgaatcac
     6001 aaccgttttg accgtagtat ttttagcgtc agactgaggt gaaatcggat tgacttcaat
     6061 cacttcatca cgataattcg ttagtgcgtt ggaataagtt ctcactagca aagtttgaaa
     6121 ttcatttact agttcctttt gttgttgggg ctctactttt gaccagttct tccccatcgc
     6181 aagttgagtc atccgtgtaa agttaaagtg cggtagtatt ttattttcaa ccaaatcaag
     6241 aatacgatct tttccaccct tcttgatccc attatcctgc ttgatgatag caatcacttc
     6301 atcaaccgta tttttaacta gtagatcagg cgcagtatcc tttgcccatc ctgataaagc
     6361 cacacatagc agcatcaaag aaatcaatgt attaatcatt tttttcataa ttatctcaat
     6421 cctcaaaatt atccgataag tgtattaatt ctcagaagct ttatcaaaca gaaagcgtcc
     6481 tatcatctct tctagcacca tggctgaatt tgttttcata attttatcac catccgcgag
     6541 cattacagca tcgccccctg gtgacaaacc aatatactgt tctcccagta atccagacgt
     6601 cagaatacta gcaaaggtat cctttggaaa ttgataagaa ctgctgactc ccattgtaac
     6661 aattgcatca tgggtttcca gactatattg aatatccaat acacgcccta caacaacacc
     6721 agcactttta acaggcgctc gaatttttag gccgccaata ttttcaaaat ttcctgttaa
     6781 attatacact tcccctgcat tatagcttgt caagttccct acttttaacc ctagaatcag
     6841 cagggctcca ataccggcca tcacgaacag cccgacccag aaatccatta ctgttcgtga
     6901 catcaactca ctcctctgaa cataaatgaa gttaatacaa aatctaatcc aagaataacc
     6961 aatgaagagg tcacgactgt tcgagtcgtc gcgcctgaaa caccttcggc agtaggtggg
     7021 gcatcgaaac cttcaaaaac cgcgatcgct gtaaccgcaa cgccaaaaaa gcagcttttg
     7081 atcacaccat ttacaatatc atagcgaaaa tcaaccgcgt tctgcatctg tgaccaaaat
     7141 gatccattat caaccccaat aaaaacgacg gtaaccaggt atccgccgaa tactcccatt
     7201 actgagaaca tggcagccag aaacggcatt gatatcacac cagcccagaa acgtggcgct
     7261 actacccttg caatcggatc aactgccatc attcccatcg cagctaactg atttgttgct
     7321 ttcattagtc caatttcagc agtaatagcc gatccagctc ggctcgcaaa caataacgcc
     7381 gccatcacag gaccaagctc tcgaaccaaa gaaagcgcaa ctaatgttcc cactgcagac
     7441 tctgaaccat atttctgcag tgtttcgtag ccttgtaaac caagaaccat tccaacaaaa
     7501 aggcctgata caagaataat aatcagtgaa agaacaccaa caaaatagat ttccctaacg
     7561 acaagaccaa atcgcaaaaa actagttgtt gatttgagta aaacccaaac aaaaaaccga
     7621 cttgcaacgc ccaaacgcca aatgctatca atcgtccggt gtcctatcgc ttggatacta
     7681 accacaatgc ggttacgcaa caaaattctc cagcgccagg tctttttgat aagattgagc
     7741 aggataatgg aatggtacag gaccatcctc ttcaccatgc acaaattgat gaacaaaagg
     7801 tgcgaccgag ttttttacct cttgtggagt gccttgcgca gcaatcacgc catccgccaa
     7861 aaaataaaca taatctacaa ttttcaaaga ctcttggaca tcatgtgtaa ccacaattga
     7921 tatcattcct aatgcatcag ttaatcgtcg gatcagatta ccaattacgc tcaatgaaat
     7981 cgggtccaaa ccagtaaagg gttcatcata cattacgagc atgggatcaa gtgcaattga
     8041 tcttgctaaa gcaacacgac gtgccatccc accggataac tctgctggca tcaagtgatg
     8101 tgcacccctt agccctacag cctgtaattt catcaaaacc aggtctcgta ccattgattc
     8161 tggtaaggta gtatgctcac gtatttgaaa agctacgtta tcaaagacag agatatccgt
     8221 gaataaggca ccaaactgga acagcatccc catctttctt cgcaacttaa acaattcatc
     8281 ccgactaagg tgatgaacga tttgatctgc aactttaaca tgaccaccag aaggtttaat
     8341 ttccccacca atcaaacgta ataacgtcgt tttccccgag ccactcccgc ccatcactgc
     8401 aatcactttc ccacgcgaca tagccatgtt aacgttattc aaaattggcc gtgtgccata
     8461 tgaaaaggct agattcttga tctcgatcag attatctgtc atgagattct tctgttactt
     8521 gtaatatcac gaaaaaatag cattaagcat aaaacaaggt attctaattc agaacctgaa
     8581 tcgatggaga ttttcttcca tcaattaata ttgaaattaa tactgattaa tcaccagcta
     8641 ccagcaatgc gactttatag ccaaccttta agttttatca acttgatact cattatttta
     8701 gtaattagtg ctactccatt cagcgcacag ggcaatacaa tttataaatc aaccgatgcc
     8761 gcaggacgag tcacctattc cgatacacca acacccgatg caaggccgat ccacttacct
     8821 tccacaccag cactcacact acaccaggtc actactgtac tcgatggtga tacgatcgtg
     8881 ctcaaaggaa atcagcatat tcgcttatta ggaattaata ctcccgaaac taatacaaga
     8941 tatcgtgaag cagaagctgg tggtatcgtc gcaaaaaaat ggctacaaga aaaactaact
     9001 ggagtaaaag tctatttgga acatgatcaa ctgaaaacag atcattacaa tcggtcactt
     9061 gcgcatgttc gtacaaagaa cggggagcac atcaatttaa ttctagttga aaaagggtta
     9121 gcatttgtta atttgatacc ccccaatctc cgccatgctg atgccttgat tagcgcccaa
     9181 agaaaagcag aaaaagaaaa gctaggcatt tgggcttatc ctgactacgc accttttccg
     9241 attactcaaa ttgcaaataa aaaaacacgt agttggcagc gcttttacgg aaaacccatt
     9301 tcaattaaac gtagcaaaaa atacagccgg ctctttttta gtagcaatat cgatgtccgt
     9361 tttgaaaata aatacctaac tttatttcca tcattggaga cctatttgaa taaaaccatc
     9421 gaagttcgtg gatgggtatc tcgtcgaaac gatcactact caatatcaat tcaacatcct
     9481 agcgcaattg ttattcgcta gactaaattt agctgcttcc ttgatttata gattttatct
     9541 ttttatctgt cagattatct aaatttccca agaatcttct attgtcagcc agattactgc
     9601 cgttaagaat ttgaaaaatt aggtagttga aaaatatatg gcaacaatac aatccaaaat
     9661 agtagctgac ttagaaaccc tctatctaga aaattcctca aaaaattgtc cagaagagca
     9721 cgtttatgaa tggcttattc taggcatcac cattgatggc cgaacattcc gaccaagcga
     9781 ctgggcagag cgcctttgcg gcgcaattgc acaatataac aatgggcgct taatctattc
     9841 agagttagct tatccaattt cgcgcaacgg acaaattggt atagtgattg aaaccttact
     9901 aagaagcgaa aacccaacca tctattcttt ttttatggac tttgcccgag ataatcgact
     9961 aaaaattatc tccggtagag aagcccctcg ctcactcaat accaactcca ctcaaaacaa
    10021 tcgccctctc cgtttagtta tttaattaag tgaaatctca tcacaaatct gaccaatttt
    10081 agtatgtcta gctttataaa ttaactactt cggctctatc tgagaaagat gctgagctac
    10141 agataacctt gcacctagcc atccaagaaa ccctgcaaca gcgcctaaca ataaaacctg
    10201 aaaaagagat aaaggattca agcgtaaact taactcataa agttgtgaca attcagttaa
    10261 attatcattg atcttttgag ccccaacagt aatgataatc caagctatca aagcacctac
    10321 cactccttgc aatgcaccaa aataaagaaa gggacgtcgt ataaaacgat ccgttgcgcc
    10381 gatcagtttt gaaacttcga tctcttcttg ttttgtaagt atttgcaacc gaattgtgtt
    10441 aaatatgaca attactagcg ccacaccaag caaagaagac agcatgataa ctgcaactct
    10501 tcctaattta acaaaagcat gcacttttct ggtccaatcg gagtcaactt gaacatatga
    10561 caccgctggc cactgctgca aagttagtcg attctcttct aacttatcag gtggcatatg
    10621 ttgcgtatga ataataaaag catggggcaa tggattcttt tttaaatgaa gcgcaatatc
    10681 tcccatccca ctttcgtgtt gtagctgcgc taaagcaatt tcattggaaa caaattgaaa
    10741 gctcgtaata ccatcaaaac tttccaatcg tgaattaata ttttcaatat cagactcagt
    10801 cgcatcaatc tcaagaaata ggcttatctg aggtgaatgt acaaaatggt ctgaaaaact
    10861 ttgtaagtta tctaataagg tataaatccc taacgggaag ctaaatgcaa taccaattac
    10921 tgccacgctt agaaaatttg agacaggctt aaaaaaaaat tgctttgcag ttaaccccaa
    10981 agcttgccaa tgttgagaaa tccatattct cataccataa gatgtccttg attgagtgaa
    11041 agtatacgcg cagttgaatc attcaaaatc gtttgatcat gggttgctat taataccgta
    11101 actccaactt gatgaaatga tcgaaaaaca tccataatat tttgggcata tgccacatca
    11161 agatttgctg tgggttcatc agcaatcaag attaaaggcc gatgaacgac agctcgcgca
    11221 atacacaggc gctgcttttc tcctccagat aacgtaatgg gatacgcctt ttctttattt
    11281 agtaacccaa ctttatctaa tgctgcacgc actcgcctct ccatgacact cttttcgaac
    11341 ccatgaattt gtagtggtag catcacatta gcaaatactg tgcgatcata taaaatcttt
    11401 tgatcttgaa agataatccc taggctacgc cttaaaaaag gaaccatgct tttcttcagt
    11461 ttattcacaa tttgatcact aaccgtaatg cttccagatg ttggtttttc aattgccgca
    11521 atcaatttta gtaacgtact ttttcctgcg ccagaatggc cagtaataaa taccatttca
    11581 cctgtatcaa tcgtaaaaat gagattcgtt agcgcttcat agccatctgg ataacgctta
    11641 taaaccttat caaaagtaat cattacaacg taaaactaat cgaataaagc ctcaacaaat
    11701 tcaattgcat caaagggcct aagatcatct aagctctccc caataccaat aaaacgtaaa
    11761 gctggcggat tgtttggata ttgcgctgca atcgctgcaa taacgcctcc ttttgctgtt
    11821 ccgtcaagct tcgttaaaac cagacccgtc acatttaaag cttcattaaa ggttttgact
    11881 tggcttatgg cattctgtcc tgtgttagca tccagtatga gcaatacttc atgcggtgcg
    11941 tcaggcagcg ctttattgat gacacgcttt atttttttta tttcttccat taagtgcatt
    12001 tgcgtagtca atctacccgc tgtatcagcc aatacgatat caatatttct tgcctttgcc
    12061 gcattgattg catcaaaaat aacagctgca ggatcacttt tcccgggaga atcgctttct
    12121 tgcgcgatca ctgtcacatt attacgctta ccccattcga ccaactgctc tcgtgcagcg
    12181 gcgcggaaag tatcacctgc tgctaataga actgattttc cttgagactg gaaataataa
    12241 gcgagcttcc caattgttgt cgtcttccca acaccattta cccctgcaat catgattaca
    12301 aaaggttgat gagtcgatat atcaaggggt ctcgctagcg gttgaagcaa ttcaatgagt
    12361 gtttcttgta aaactccctt caattgatga gtatcactta attgatcacg cttaaccttg
    12421 ccccgcaaag agtctaacaa ctgattggtc gcctgtatcc ccacatcagc cgtaagtaga
    12481 attgtttcga gctcttcata gacggcttca tcgattttcc tagctccaaa taagctcgat
    12541 aattgcttac ctagattttc tctagttcgc gctaaccctt gcttaatctt gctaacaaag
    12601 cttactgact gtgattgatc ttctggagga tttttctggg atttaaaaaa actgaacatt
    12661 ctgataggct tcaacgattt taattatgat taaaagctaa ttctatgcct attccctatt
    12721 tttgggtatg tgtaaataat aagcgtcacg ttcaatgcga tacaaacagt taaaaattga
    12781 ctaatcattg cgacatcaca aacaattcaa ctctcgaatg tcacgcccaa tcagcgctca
    12841 aataaattta gccgcgttaa gacacaatct ttccatcgtc cgtcaatacg caaaacattc
    12901 acgaatcatg gctgttataa aagccaacgc ttatggtcac ggattaatac gcgtagctaa
    12961 aacacttcac gaaatcgatg cttttgcttt acttgaatta gaggcagcaa ttcaactgag
    13021 agaaatcgga attcatcaac ctatattgtt gctagaaggt tttttttctg aggcagacct
    13081 tatatcaatt gatcattacc aattagatac cgtcattcat caccctgagc aaatagccat
    13141 gttagcaaag catcgagcta acaaaaaaat aaatctattc cttaaaataa atactgggat
    13201 gaatcgatta ggactcgctc ctcgagaagg cactaaagcg atccatacct taaagaattt
    13261 atcgcacgtt ggtgaaataa ccttgatgac gcacttggct tgtgcggatg accctgcagc
    13321 cgatcgtgaa atcaacgacc aattaaatat tttttcagaa atacttaata ttactcggtt
    13381 accttgctcc ttagctaact ctgccgcaat tatccagcat ccccaaaccc atcaggactg
    13441 ggtacgccct ggcatcatgc tctatggtgc atccccttta atccataaaa cagccactga
    13501 acttgatcta aagcctgtaa tgaccgtttc aagcaaaatc attgcactgc aacaactcaa
    13561 tacaaatgat aaagttggat atggtggtac tttccgagcc caacaaccga tgcaaattgg
    13621 aatcgttgct gctggctatg ctgatggcta tccgcgccat gctcaaacag gtacgcctat
    13681 catcgtaaat ggtcagcgta ctcgtattgt gggccggata tcgatggata tgcttacagt
    13741 tgatttgaca ggcatcacga acgtcactgt cggcagccca gtcatattat ggggtaacca
    13801 tttacccatc gaagaagttg ctaaatcagc gaatacaata agctatgagc tattttgttc
    13861 actttcttcg agggtaaaag taatcaccac tgagtaattt tgagaagcca gcaagatttt
    13921 gttgactcac tggctttttc tggcttttat tctactttcg cactattgcg caaattttga
    13981 atagttgaag caaaattacg ttgtaaaaca cgctgctgca tattcatttt cacttcatcg
    14041 aatggtggag cgacagcagg acgaacatct tccagtcgaa caacgtgcca accaaatgat
    14101 gtttgtacag gttgatctgt cgtattaccc ttcttcaaat tttgaagagc atcagcaaat
    14161 ggcttaacat aggctccagc agaactccaa cctaattcac cgccattact tttacttccg
    14221 gtatcaattg atttctcctt agcgagctta tcaaaagccc ctcctttctt gagcgcagca
    14281 ataatatctt ttgcttcttt ttcagtttct actaggatat gccgtgcttt atattccttg
    14341 tctcccatct gcgacttgac ttcctcatat tcgcgacgta gaacgtcatc ccctaccgca
    14401 ttatttttta tatactccgc ttgatatgcc ctaacaagta ttgcttgtcg cgcaagttcg
    14461 atttgggtca tcacatctga acttttatca agcccgcttt ttatcgcttc ttgtgcaacg
    14521 atctcttctg caatgagatt ctcacgaagt gagtttctta attcagttga gtcttgctgc
    14581 ccttgggcaa tcgctgcttt aaccataaaa tctaggcgcg cttggggaat cccaacccca
    14641 ttcacttttg ccacagtttg tgcaaaaaga gaagaggtta taaccaaacc agtgatcata
    14701 actaacatta acaacaaaaa ttttctcagg tacatgcaac ttcctctatt ttagtaattg
    14761 aaaacaaatc gctactctca actccatcac acgccatgga ttttacatat tataagccga
    14821 gccttaataa aattttaggc cttcatggcc aaaacgatta attttatctt tgtcacgttg
    14881 gaaaaacttt tttaaatggt ttaactgtca catgcagata cacgccagct aatacataag
    14941 gatcagcatt agcccatgcc tgggctactt ccaatgattc aaattccgca acaattaaac
    15001 ttcctgaaaa cccggctgga cccggatcaa cattatcaat tgctggataa ggccccgcta
    15061 aaaccagcct accctcttgc tgcaatactt ggaggcgttc caaatgacca ggtctcgcaa
    15121 tcaaacgttt ctcagaacta ttcggaacat cttccccatt gataacgtat aacaccttca
    15181 accatccttc actttatgat agaaatttat aattaaatgc cagataagcg aactgttttc
    15241 tttttatatc gaattcttct attttctttt gggtcacact gcaagggtcg ataaatctcg
    15301 acgcgatcat tgttttgcaa cacagcatct aaagtagtta attttccaaa aatacctatt
    15361 ttctgtgaaa cccagtcgat atcaggaaag cgtttatcaa attctgataa tgtaatcgcc
    15421 tgctcaaccg tgataccact aggcacggtt attcgttgaa gaaactgatg tcgcgaaagc
    15481 gcataagcaa tctcgacctc gatatttttt tcactgagaa atgccataaa ttttttcggc
    15541 tcgcttaata aaagcatcaa caaagctatt tgctattcca aaaaaaacag gccctactaa
    15601 cttttcgagt aatcgattag aaaaagtata gtgtagatta aattcaattt tacactcatg
    15661 ctctgacaac gaaataaatc gccattcccc gtcgagttct tcaaaaggcc catccagtaa
    15721 tttcatttta atcaattcag gaacctgacg cgtattctct gtagtaaaac tatgtctaac
    15781 aggcccataa ttaatattca ccgtggcatg aacaatggta tcacttagct gttttactga
    15841 cgtaccatcg caccaaggca aaaattctgg ataattctca acagtatcca ctaatctaaa
    15901 catctgacct gctgagtaat caacatacac aacttttttg atttctgcca tacaattcaa
    15961 tgatcctttg ttttcaactg agttaagact ggaaaactga taaacttatc aattttaagc
    16021 tgctttcctt tattcttgcc aattcccgat gagtatcgca caaaataaaa aagcttttca
    16081 tgactatttt atcgaagaaa aatatgaagt aggtattgtt cttgaaggtt gggaagtcaa
    16141 atccattcgt gctggacgtg ttcaattaaa ggaagcctac gtcattattc gaaataatga
    16201 attatatttg attggaagtc atattagcgc tttacctact gcatcaacgc atattaatcc
    16261 tgatccatta agaacgcgca aattattatt acacgcagaa caaattaaac gattaattgg
    16321 caaagtcgaa cgagctggtt acaccctcgt tccacttgat atgcattata agacgggcag
    16381 aataaagttg gaaattggtt tagccaaagg aaaaaaacaa tacgataaac gtgaatcaga
    16441 aaaacaaaaa gaatgggacc gtaccaaaca acgattgtta cgggcccgat aattatttga
    16501 ttaaaaactc attaactttt aaagaattat gcaaaaacct atcgccatct ggctatttat
    16561 ctgttgtgcc cttgtctttg caatggttgt tgtaggcgga gtaacccgac taaccggttc
    16621 aggtttatcc attgtggaat ggcagccgct aattggagcg attccaccac tcagcgaagc
    16681 tgattgggaa attttgttag aaaaatatcg tcagataccg caatatcatg aagtaaacaa
    16741 aggcatgacg ctagaagaat tcaaagggat tttctggtgg gaatattttc atcgcttgct
    16801 aggacgtgct atcgggctcg tattcttcat tccctttctt tattttttga tacgcaagca
    16861 aatcgatcgc cccctcggtt taaagctctt gggtattttc gtgcttggtg ggctacaagg
    16921 cttgatgggt tggtatatgg tcatgagcgg tctcgttaac aatatctatg tcagccaata
    16981 tcgattaaca gcacatcttg gattagcgtt tatcatttat gcggcaatgt tctgggtagc
    17041 gacaggtttg ctatcacctg ctaagaaaca acaacagcct gaaacagaaa gttattattc
    17101 ccacttacgt cgttattctt tttatctgac actgctgatt tttatcatga ttttatcagg
    17161 cggctttgtt gcaggtatcc acgctggaaa agcctataat actttccctt taatggatgg
    17221 ccatcttatt ccgccaggct tgtttgtact agagccctgg taccgcaatt tttttgaaaa
    17281 cataacgacc gttcaatttg atcaccggtt gattgcttgg ctacttgcct tcttcgtccc
    17341 atttttctgg ttgcgatgta ttaaatactc actttctcca tccgcaaaaa tggcatgcca
    17401 tttgttgcta ctggctttaa ttttacagat tagcttaggg gttgcgacgc tattatcagc
    17461 agtaccctta acgcttgctg ccactcatca agcaggagcc atggtgttat ttactgcttc
    17521 gctctgggtt aatcgtaaat tatcatccat atgattaagt atctgactgg gaatgtagct
    17581 gatagtgctc agagcagcag tagaaatggt tatcagctac tatggcttct tttttaggga
    17641 tatggatttc gcaatagcga cattgcacca tgtcttcaat cccatcaatt gaattgtggt
    17701 gggttttagc ttttaatctc tggagacttt tcagcaccca gaaaataaac caaatcacga
    17761 taagtagtac taaccattta cccattccca catttcctga attgttcctt cgcgcataaa
    17821 aacaaggttg agtacttcaa ccttgtaata gtcaaataat acttgagtta atctgatcaa
    17881 tcaccattcc aagtaattac cgagcgatag gtttatacct tatccgttta ggtttggcac
    17941 cttctccgcc aagtcgctga cgtttgtcgg attcatattc ttggtaattt cctgcaaaaa
    18001 aagtaacttg cgaatctcct tcgaacgcta gaatatgggt cgcaattcga tcaagaaacc
    18061 accgatcatg agagataacc aatacacacc ctgcaaattc tagcaatgca ttctcaagcg
    18121 cacggagtgt ttccacatcc aggtcatttg aaggttcatc cagtaataaa acattccccc
    18181 ccgatatcag cgttttagcc aaatgtaacc gacctctctc tccacctgat agctgaccta
    18241 caattttctg ttgatctggg cctttaaaat tgaatcgacc aatataagct cttgatggcg
    18301 tttcgtattt accaaccact aaaatatcat taccacctga tattgcttca aaaacagttt
    18361 tatcactttc cagaacatcg cgtgattgat cgacataggc aaccttgact gtttctccaa
    18421 ttttaatttc ccctgaatca ggtttctctt tgcccatgat catgcgaaac aaggttgatt
    18481 ttcctgcacc attggggcca atgatgccaa caatggcacc gggtggaatc ttgaaactaa
    18541 gattatcaat cagcagtcga tccccaaaag ctttagaaac atccatcaac tcgatcactt
    18601 cattcccaag tctgtcggct actggaataa aaatttcctg agtctcgtta cgcttctgat
    18661 actctaaaga attgagttct tcaaaacgcg caatcctagc ttttgattta gcttgtcgtc
    18721 ccttaggatt ttgtctaatc cactccagct ctttctgcaa tgccttttgc cgcgccgatt
    18781 ctgacgattc ttcttgtttg aggcggattt ctttttgctc tagccagctg gaataattac
    18841 ccttccaagg aattccatgg ccacgatcaa gctccaatat ccactctgct gcgttatcga
    18901 gaaagtaacg atcatgggtt accgcaacta cagtccccgg aaatcggacc aaaaattgtt
    18961 ctagccattc cactgattct gcatccaagt gattcgtagg ttcatcgagc aacaacatat
    19021 ccggctttga aagtaataag cgacacagcg ctacccgccg cttttctccc ccagacaagt
    19081 tacgaatcac tgcatcccat tcaggcagac gtaatgcgtc agcagcgact tccatttgtt
    19141 gtgctgtatc accgccaccc gttgcaataa tggcttctaa tcgtgtttgc tctgccgcta
    19201 atgcgtcgaa atcagcatcc ggatcggcat aagccgcgta gatttcatct aatttctgtt
    19261 gcgcactggc gatatcacct aatccctcct gcacagcttc cctaaccgtt tgatctggat
    19321 taagttgagg ttcctgaggc aagtagccga tttttaaatt aggcatcggt gtgacttcac
    19381 cttcaatgtc tttatcaaca ccagccataa ttttaagcaa agtcgattta cccgagccat
    19441 ttaggccaag caagccaatt ttcgcgcctg ggaagaaact aagagaaata tctttaagaa
    19501 tcgtacgttt aggcggaaca attttgccaa cacgattcat cgttaaaaca tattgagcca
    19561 taaatatggg taaaagtgtg agttattaaa agtttaaatt taggaaattt atctaaaatt
    19621 ctattatctg ttaatcgata agtaatgaga tgccgaatat aaccatacat ttatacgttg
    19681 caccgatcca aaattgtttt taagcgatta accgctaaac atttttacga ataagcgcta
    19741 actaggagaa agttatgaag gtaatggtaa tttttaaaac gggcaccagt caggtattta
    19801 tcgttcctcg cgatattcta gcagttgaat tcagacgatt ggcagaatca gtcggtggtg
    19861 aaatccaccg catcgaattt atgcaaaaga ataaatttgc tgctcctaaa tatgcattaa
    19921 ttaaagatat ttaagattca acgaattcat aatgattcaa aaacgccagc agcgcccatc
    19981 ccgctcccga tacacatcgt aaccattcca tatttttgtt tatgccgacg cagcccatgt
    20041 aacagcgtcg cgactcgtat tgcgccagtt gcaccaagcg gatgaccgag tgcaattgct
    20101 ccccctaatg gattgacttt attacgatcc agtcctaaat cacgaatcac tgccagactc
    20161 tgcgcagcaa atgcttcatt taattcaatc caatcgagct cctcttgctt caatcccgtt
    20221 tgttttaata ctttaggaat agctttaatc ggccctattc ccattatttc cggtggtacg
    20281 ccagcaaccg cgtagccaac gaaacgccca atgggttcaa ggctaaatcg ttttagagct
    20341 gcagcactca taacaaccac ggcacctgca ccgtctgaca tttgagaact gttaccagcc
    20401 gtaacagtcc catctgctgc aaatactgac ttcagcttcg ccaaggcatc taaactggta
    20461 tctgcgcgcg gcccctcatc cgtgtctctc atcgttttaa ctgtgatgac ttcatgtgat
    20521 gacagatcag gtctattttc ctcaatggcg tagggcgcaa tttcatcgtc gaattcgccc
    20581 atatcgattg cattgatagc acgcgtatgg ctagttagtg caaattcatc ttgctgatca
    20641 cgggtaattt tccactgttg agcaactttc tcagcagtca tacccattcc ataggcaatt
    20701 gcaacatgct cattatgagt gaacattgca ggattcatcg caactttatt gcccatcata
    20761 ggaaccatgc tcatactttc tgttcctgca gcaatgatca catccgcttc tccaagacga
    20821 atgcgatctg cagcgatcgc aaccgcttga agccctgagg cacaaaagcg attaaccgtc
    20881 attcccggca cactatccgg caatccagca agtaacgatg ctacacgcgc cacattgata
    20941 ccttgttccg cttcaggcat tgcacaacca gcaaccacat catcaatggc agcaggatca
    21001 agaccagtac attgtttcat aaccgcttgc aatacatgga caagcatgtc atcaggccgc
    21061 acatttttaa acatgccacg gggcgcttta ccaacgggtg tgcgaacggc agcaacaatg
    21121 taggcatcct gagtttgttt agtcatcgct tttctccatt aaacgaataa tcgcttcact
    21181 attttatcgc taattgcgaa gtggtttgcc ggttttcagc gtatgtgcaa tccgggcttg
    21241 ggttttttca gtcgctagaa gttcaataaa tgctgtacgt tctaatcgca gaaaccactc
    21301 ctcatcaacc aaactccctg gggttagatt gccaccacac atgacatgag caattttgct
    21361 cccaattaag tagtcatgtt cagagataaa atgcccttcc agcatattca ccagcatccc
    21421 tttgatcgtt gcaaccccag ttgatcctgc gacgggaata tcacgaaatt gcaatggagg
    21481 acgataacct gcctctgcca tcgcattagc ttgtgttttt gcgacatgca gcaactcaaa
    21541 ccgattcatg acaatcatgt cagcttggcg caaatagcct aattctttgg cttgttctgc
    21601 agattttcca agttgagcca tcgccactgt ttgaaaataa tgtttcaatt taggaaatgg
    21661 atcaccgtca accgattctt tagcggccct taatgccaat tccttgcatc ctcctcctgc
    21721 aggtagcaac ccaacgcccg cttccaccag gccaatataa ctttccaatg tggcaacagc
    21781 tcgatcacag tgcataacga actcacaacc gcccccaagc gcaagtccat ctactgccgc
    21841 aacagttggg atcattgagt agcgtaaatg ctgtgatagc tgctgaaagc gtgtaatgag
    21901 cgtttccact tcagcgagtt tgctagccat aagtacatct gctagatcta aactacgtgc
    21961 ggcctgtaaa atcgtagact gtgcagtcct tttgactttt tttacaaatt gatcaaatgg
    22021 tgagggaggc tcatcatttt tgggcttctc agttgctttt tgtagattag cacccgctga
    22081 aaaaggtggc tctgtttgcc aaatgacgag ggcactccaa tggtgttctg cctcttcaat
    22141 cgcttgctgc aaaccatcca aaacttcgat atcgattgta ttcattttgc tcttaaaact
    22201 aacaatggcg atatcatctc cagtgtgcca catcctgact gcattatttt caaaaattgt
    22261 ttctccatag cgatgttctt cgccaatcag tcgatcagga aagggctgcc gccgatatac
    22321 ggataaggta gatcgaggtt gatatccctt tgttaggggc gcaaaagaac ccattgtacc
    22381 atggactccc gggggcgcag ttttacccaa ttccattacc caatcaggca agggagtctg
    22441 actcatggca cgaccagccg caatatcttc attaatccat ttcgtgattg gtacccaacc
    22501 agctgcttgc caaatttcaa aaggacccaa tgtccagcca aaaccccaac gcacagcaag
    22561 atccagatca cgcgcattat tagcaatttc tgctaggtga acggcacaat agtgaaataa
    22621 atcgcgatga atcgcccaca aaaattgagc ctgcggatga gggttctccc ttaattcaga
    22681 aaattttcta gcaggatctt tttgtttgag taactgttga atatcctcat cgatcgtagc
    22741 agttgaattt tcatattcat ttttcgcagg attaaatacc aaaatatctt tatttttttt
    22801 ctgatacacg ccacggctcg atttttgacc aagcgcacct tgtgtaatca attgctgcaa
    22861 ccatcccgga agagcataat agctatgcca aggatcctgc gctagtgaat ggcgcatagt
    22921 ctctaccaca tgcgataaaa catctaatcc cacaacatcg gctgttcgga atgtagcact
    22981 tttaggacgg ccaatgagcg ttcccgtgag ctgatctact aaatcaaagc ttaagcccat
    23041 tgcacttgca tgatgtatcg ttgccaacat cgaaaacaca cccactcgat ttgcaataaa
    23101 attaggtgta tctttagccc gtatcacccc tttaccaaga taagtggtta aaaaggactc
    23161 gagatgatcg agtgttgttt gatcactata ggtacaagga atcaattcga tcaaatgcat
    23221 gtagcggggt ggattaaaaa aatgaattcc acaaaaccga tgttgcaatt cttccggcag
    23281 cgctgctgcg agctgattaa tggaaagtcc agaagtatta cttgctacca aagtatgcgg
    23341 tgcaagataa ggaattatct ttgcgtataa ctcggttttc caatcgacgc gctctgcaat
    23401 ggcctcaatt actaagtcac aatccgtcaa cgaaccaaga tgctgatcat aatttgccgg
    23461 atggataaaa gtgcttttcg aaatcaccga taacggtgac ggctctagtt tttttaaccg
    23521 ctcaatcgct tttgttacat ttgcattagg attttctgct tcagcgggta attcaaataa
    23581 taaggtctcg atatttgcat tcacaagatg tgctgcaatc tgtgcaccca tgacgccagc
    23641 gccaagaact gctgccttgc gtaccataaa gcggtgatca ctcaattttc ttcctccatg
    23701 tgttatgctt tgctaaaagg ggttcctaga atccataatc ttagacaccg aacccttcac
    23761 tggcgaccgc tttcattacc ctctttgatg aatagagtgc atcttaaccg gaaccagaag
    23821 cgcagagcaa tgctaaatca aatatatcgc tgcaatttta aaaataatat cgcttaattt
    23881 ccacgcagta agcgaaccac actcagcaaa cgaacccaac gactttgatg attttttttg
    23941 agtgcggtga tatgatgcaa attctgtatt accttttagc actgaaattt caagtcaatt
    24001 gaaatttgac gtcgttagtt tgagagggta tattaccagc cttctcgggg tgtagcgtag
    24061 tctggtagcg cgcctggttt gggaccagga tgtcgggagt tcaaatctct ccaccccgac
    24121 caattattgt aatgcccgtt ttgagactcg tgcccgtagc tcaatcggat agagcaccag
    24181 ccttctaagc tgggggttac aggttcgatt cctgtcgggc acgcca
//
LOCUS       DAOQKQ010000013        24174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_870_length_24174_cov_6.166093, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000013 DAOQKQ010000000
VERSION     DAOQKQ010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 24174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24174
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_870_length_24174_cov_6.166093"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2059
                     /locus_tag="PKZ49_01690"
     CDS             <1..2059
                     /locus_tag="PKZ49_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HND35302.1"
                     /translation="NVFFVLLLLGIVGFLLWRLRKRLKTRVFGSRLAFRLLVVFSLMA
                     ILPGALVYGISVQFLGKSIESWFDVKVDQALEGGINLGQTMLDNLLEELEKKAKVTAL
                     ALADSPSQTLSSLNQLLAHSYIQEATLFNQDGKAIAFSSLDDVALFPEIPNATAMRHI
                     RMQKSYSAVESIPNKGLFLRVLVPVNVLSLEDDIRVLQVLQQVPSGLAENAEIVQTGY
                     RNYQELVLSKQGITRLYSVTLTFALLLALFSALASASLISEKLSAPLGMLAEGTRAVA
                     QGDFSRRHQVFSNDELGILTESFNLMTEQLDLARVNAQQSQHEIESARAYLESILANL
                     SSGVMVFDDKLKIRTVNRSAEQILQIPLIYLEGLAIDECVEKEPKFHLLASEIQNGFG
                     STQSGEWQRQVLHFSESKDQVLLLRGTRLPESSGGGGIVVFDDITGLLQVQRTVAWGE
                     IARRLAHEIKNPLTPIQLSAERIQHRLMDKLSGQDAKILQRSTETIVNQVEALKKMVN
                     EFSEYARIPELEMVQIDFNRLIHEVLALYETSNAKAENSQCPEIKLALSSDLLLIRGD
                     PARLRQVIHNLLQNAQDALADTKNGTITVSTDMISGNVRLCIIDNGKGFAEEIKTLAF
                     EPYVTTKPRGTGLGLPIVKKIVDEHNGAIQIENTQPHGAKVTITLPAINAGQVASTMN
                     RLA"
     gene            2123..3385
                     /locus_tag="PKZ49_01695"
     CDS             2123..3385
                     /locus_tag="PKZ49_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HND35303.1"
                     /translation="MANNTILVVDDEIGIRELLSEILSDEGYRVVLAENAEQARVCRN
                     QSRPDLVLLDIWMPDTDGITLLKDWANSGVLTMPVVMMSGHGTIDTAVEATRIGAVDY
                     LEKPIPLQKLLSTVKQALRGGQSKPALTISLANLGKGPQIVELKKKLEQIVNLVSPVL
                     LVGESGVGVEHCARFLHRVNTPWVEPEQLSFLADNPVGILEQVKNGLLFFREIGGLNK
                     LEQKGLLLLLGKLEKFNVRLVCATTQPLAELTAQGTYNSKLYEILSNLTINVPSLRTH
                     REDIPELANQLLSTSIETGEVPFVHFSTAALNTLRNYDWPGNLAQLKSVVHSLALTCT
                     GSEISAEEVQQVLTPPLSPTMSATPDIPLNVSLREARDLFEKNYFEKLIDQESGNMTR
                     VAERAGLERTHLYRKIKMLGIKLRGHGG"
     gene            3524..5539
                     /gene="tkt"
                     /locus_tag="PKZ49_01700"
     CDS             3524..5539
                     /gene="tkt"
                     /locus_tag="PKZ49_01700"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HND35304.1"
                     /translation="MGAFGDFKAPVFKNLTSAIRALAMDAVQKANSGHPGMPMGMAEI
                     AEVLWIHHMRHNPSNPKWADRDRFVLSNGHGSMLIYALLHLTGYDVSIEDIKNFRQLH
                     SKTPGHPEYGYTPGVETTTGPLGQGITNAVGMALAEKILANEFNRPGFDIVNHYTYVF
                     LGDGCLMEGISHEACSLAGTLGLGKLICFYDDNGISIDGHVEGWFTDDTPKRFEAYGW
                     HVIPNVNGHDPEAIEAAIEEAKKKQDKPSMICCKTVIGMGSPNKANTHAVHGAALGDE
                     EIAATRPHIGWHHLPFEVPKEVYDAWDARTKGKKLESEWNQKFSEYSQKYPKEAAEFT
                     RRVNGDLPKDWSAYVDGVVGRVNAKGETIATRKASQNAIEGLAPALPELLGGSADLAG
                     SNLTLWSGSKGVSSESGGNYIYYGVREFGMSAIMNGLSLHGGFIPYGGTFLMFSEYAR
                     NALRMAALMKIRSIFVFTHDSIGLGEDGPTHQPVEQTATLRLIPNMDVWRPCDTVEST
                     AAWARAIERKNGPTTLIFSRQNLPFQKREADVIKLIDKGGYILSEAADGKPRAIIIAT
                     GSEVGLAMAAQKTLADEGIHVRVISMPCTNLFDRQDKAYQEAVLPKGIKRVAVEAGVT
                     DYWRKYVGLEGEIIGIDTFGESAPAEVLFKHFGVTTENVVNAIKRII"
     gene            5609..6610
                     /gene="gap"
                     /locus_tag="PKZ49_01705"
     CDS             5609..6610
                     /gene="gap"
                     /locus_tag="PKZ49_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HND35305.1"
                     /translation="MAIKVGINGFGRIGRMVFRASIEKFSDIDVVAINDLLEPDYLAY
                     MLTHDSVHGRFSGTVAIEGNTLVVNGKRIRLTAIKDPAELKWNEVGADIVVEATGLFL
                     TKETCEKHLTAGAKKVIMTAPSKDDTPMFVYGVNDKAYTGQTIVSNASCTTNCLAPIA
                     KVLNDTWGIKRGLMTTVHAATATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGV
                     VIPELNKKLTGMAFRVPTSDVSVVDLTVELNKDATYDEICSVMKSTSQGTMKGVLGYT
                     DQKVVSTDFRGESCTSVFDAEAGISLDKNFVKVVAWYDNEWGYSCKVLEMVKVIASK"
     gene            6682..7863
                     /locus_tag="PKZ49_01710"
     CDS             6682..7863
                     /locus_tag="PKZ49_01710"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HND35306.1"
                     /translation="MSVIKLVDLDLKGKRIFIRADLNVPIADGKVTSDARITASMATI
                     KHCLERGGKVMVTSHLGRPEEGVWSEENSLKPVADNIAARLNKPVRLIKDWVNGGFDV
                     AEGELVVLENCRINKGEKKNVDETAQKYASLCDVFVMDAFGTAHRAEASTHGVAKYAP
                     VACAGILLTEELDALTKALDKPARPMVAIVGGSKVSTKLTVLESLSEKVDQLVVGGGI
                     ANTFLKAAGKNIGKSLCEADLVPTAKVLMDKMQQRNATIPIAVDVVVGKKFDANEPAI
                     TKSADTVADDDMIFDIGPNSAQELVDIIMKAGTVVWNGPVGVFEFDQFGGGTRRIAQA
                     IAETQAFTLAGGGDTIAAIQKYDIYDKVSYISTAGGAFLEFLEGKKLPAVEILESRAK
                     Q"
     gene            7885..9351
                     /gene="pyk"
                     /locus_tag="PKZ49_01715"
     CDS             7885..9351
                     /gene="pyk"
                     /locus_tag="PKZ49_01715"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HND35307.1"
                     /translation="MFRRTKIVATLGPACSDPAVLKRMIVAGVDVVRINFSHGTREQH
                     IAFAELTRSLAHSVGCTVGVLADLQGPKIRIGKFEQGKIWLNVGDEFILDANCQLGNK
                     ERIGLDYKELPNDVEEGAILLLDDGRIVLSVNRVKGSEIFCEVVQGGPLSNNKGINRK
                     GGGLSAPALTSKDIEDIKIAADLKADYLAVSFPRSGEDIRLARSLMQEAGGNSLIMAK
                     IERTEAILALDDIMEASDALMVARGDLAVEVGDAAVPALQKRMIRSARANNRLVVTAT
                     QMMESMITNPIPTRAEVSDVANAVLDGTDAVMLSAESAAGQFPVESVEAMARVCLEAE
                     KEYTVNQSLRRSPDTPPVSIEEAIARATMYTAGNLKIRAIAALTQSGKTALFMSRRSS
                     KVPIFALSPQKDTLGKMTLFRGVYPVEFGEGLYDPEVILDLAEDELFKRGAVRDGDVI
                     IMTIGEPVGKAGGTNTMKIVRVGDCRKPLNIDNFEANI"
     gene            9445..10509
                     /gene="fba"
                     /locus_tag="PKZ49_01720"
     CDS             9445..10509
                     /gene="fba"
                     /locus_tag="PKZ49_01720"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646216.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HND35308.1"
                     /translation="MALVSLRQLLDHAAENGYGLPAFNVNNLEQIQAIMQAADECNSP
                     VIMQGSAGARKYAGEAFLRHLIAAAVEAYPHIPIVMHQDHGASSAVCVNAIRSGFSSV
                     MMDGSLEEDAKTPSSYEYNVDVTSRVVAIAHAVGVSVEGELGCLGSLESGMGEAEDGH
                     GAEGVLTHDQLLTDPEQAADFVKKTGVDALAIAIGTSHGAYKFTRKPTGDILAIERIK
                     EIHKRIPNTHLVMHGSSSVPQEWLEIIREYGGEMKETYGVPVEEIQEGIKYGVRKVNI
                     DTDIRLAMTGAIRRHLAKNKSEFDPRKFLKDATAAAKDICKARFEAFGCAGQASKIKP
                     IMLDNMATKYAKGELKAIIK"
     gene            complement(10582..12126)
                     /locus_tag="PKZ49_01725"
     CDS             complement(10582..12126)
                     /locus_tag="PKZ49_01725"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HND35309.1"
                     /translation="MTREIELKLTIPPGHIKAIAQLPILSSLSIAPPKKQKLYSIYYD
                     TPDLSLRNQRCSLRIRRIGKQWIQTIKSGGNITAGLHQHNESEIPLNKAQPDLDHLQQ
                     TGVITLAQQRKIIRTLKPIFITQFTRDTHTLQTKDGSIIEFCLDHGKITADHLNDSIH
                     EIELELKSGNSAQLFQLALDIAQLTDFPIRLENVNKAERGYKLFIGDRTPPTKAASIE
                     INTKTPFLDALSKILSSCLLQLTKNENGVLFSEDPEYLHQMRVAIRRLRSAFIIFKPL
                     LSEQHQSITFFEQELKWLMHLLSPVRNWYVFANEILPPIISSCPDVEPIKVIQKLGQK
                     QRSKYTQQVQTNLQSKRYLAFILKLNLWIYDLRHDIKLESSISIGSFSRSYLLDQHSA
                     ITKNKEKLEKMKPAALHALRIQIKKQRYAAEFFYSLYAKQECFKYYVQLLAILQGALG
                     SINDNYMIDQQLESILSSQKQKKLARPIGVIHGWKAYITTQMKADLKNNWKEFLTMSP
                     FQRNGQ"
     gene            complement(12142..14223)
                     /gene="ppk1"
                     /locus_tag="PKZ49_01730"
     CDS             complement(12142..14223)
                     /gene="ppk1"
                     /locus_tag="PKZ49_01730"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HND35310.1"
                     /translation="MNDLSNSVQFLNRELSQIEFNRRVLAQAENKEMPLLERLRFLCI
                     VSSNLDEFFEVRVAGLKEQIKLDAPGFGLDGMMPRQAFKRVSEQIHQLVAQQYQLLNE
                     SILPELEQQGIRFIRRASWNEVQRDWIKAYFFRELIPMLTPIGLDPSHPFPRVLNKSL
                     NFIVELEGKDAFGRNSGIAIVQAPRILPRIIRLPSDVSTEEHSFIFLSSILHAHVGEL
                     FSGMQAIGCFQFRATRNSDLFVDEEEIKNLRIALQGELPQRHLGDAVRLEVADSCPPH
                     MITFLLDQLSLTQDDLYQVSGPVNLVRLMRVPDQIDRPQLKFPLFIPGLPFSSQKKKN
                     NDIFHQIRKNDILLHHPYQSFQPVIHFIQQASSDPDVVAIKQTVYRTGSDSTIMEALI
                     HAASKGKEVTVVVELLARFDEEANINWAGRLEEVGAHVVYGVVGHKTHSKMAMIVRRE
                     EGRLRRYVHLSTGNYHTGTAKLYTDFGLITCHEEICADVNAVFFQLTSLGKASKLKHL
                     WQSPFSLHHQIMDAINREAENAKAGKPAHIIAKMNALLEPGIIRALYAASNAGVIIHL
                     IVRGACALRPGVPGLSTNIKVRSVIGRFLEHSRIFYFRDNNQHHVYLSSADWMDRNFF
                     RRIEICFPVLDSRLKKRIITEGLKPYLKDNVQAWEMDCNGYYKRKVSHRGKQFCAQEA
                     LLAKLVAAQQT"
     gene            complement(14311..14589)
                     /locus_tag="PKZ49_01735"
     CDS             complement(14311..14589)
                     /locus_tag="PKZ49_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HND35311.1"
                     /translation="MARIVKCIKLGHEAEGLDAPTYPGELGKRIFENVSKQAWGQWIK
                     HQTLLVNEYRLNLADIKARKYLAGQMEAYFFGEGVDKPTGYVAPSDNT"
     gene            14863..17118
                     /gene="cas3"
                     /locus_tag="PKZ49_01740"
     CDS             14863..17118
                     /gene="cas3"
                     /locus_tag="PKZ49_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HND35312.1"
                     /translation="MADNVQNQSIALAHVRKNEEGKWKEHLLHEHLERVSYLAGQSAT
                     TFGSEDWARIAGLWHDLGKYREKFQQYIKSASGYDAEAHIEDATGRVDHSTAGAIHAI
                     EKLGLQGRILAYLIAGHHAGLPDWNNAEGSQSVLFQRIQNGKQNGYLQEALDAMPPAE
                     ILNQSRPQSLPPNGSLALWLRMLFSCLVDADFLDTEAFMDDKRSGLRSWYPSIVALKS
                     AFDQHMADKAANAKDSDVNRIRAEVLRQCREKAKLPSGLFSLTVPTGGGKTLSSMAFS
                     LNHAVHHGKDRVIYVIPYTSILEQTAEIFRSIFGNDNVIEHHSNLDPDREDARSRLAT
                     ENWDAPIIVTTNVQFFESLFAAKTSRCRKLHNIVNSIVVLDEAQLLPPEFLAPILHVI
                     QDLTNSYKVSFVLSTATQPAFSPRPKFTGLKNVCELMDNPERLYADLKRVKTELPDDF
                     ASPRTWESIARELQQYESVLCIVNSRRDCRELHSLMPKDTIHLSALMCGQHRSQVITG
                     IKQRLKDGILTRVISTQLVEAGVDMDFPVVYRALAGLDAIAQAAGRCNREGTLSEMGK
                     VVVFVPPKPAAPGLLRKAQQSGQEIMRLSKGDPLTRERFEAYFRHYYESLNSLDKANI
                     VSLLDLHNQAEVRKAEFNFRTAASNFRLIDEEGQTAVIVRYGESTRLIDALEKSQNMQ
                     PHERRGILRKLQRYTVNIREHECRKLLENREIQEIFPGIHIQNTDLLYHSQLGLLMSK
                     DVVFDPTQSVV"
     gene            17131..17796
                     /gene="cas5c"
                     /locus_tag="PKZ49_01745"
     CDS             17131..17796
                     /gene="cas5c"
                     /locus_tag="PKZ49_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018409881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
                     /protein_id="HND35313.1"
                     /translation="MKIYCLEVRGDFACFTRPEMKVERVSYDVMTPSSARAVFESILW
                     KPAIRWHIDKIEVLKPIRWVSIRRNEVGAVISVRNAQEAMNKGTGCLGLNIEDERQQR
                     AGLFLRDVAYRIHAHFELQPDAGENNTPAKFLDMFERRAEKGQCVNQPYLGCREFACD
                     FRLIDPMLEQSNPVKETRDLGWMLYDMDYSNAADPQPRFFRATLENGILNVPVWNSQE
                     VRG"
     gene            17793..19505
                     /gene="cas8c"
                     /locus_tag="PKZ49_01750"
     CDS             17793..19505
                     /gene="cas8c"
                     /locus_tag="PKZ49_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HND35314.1"
                     /translation="MILQALNEYYQRKTRLPDSNLAPPGFEHKPIPFLIVLTVDGHFA
                     GLDDTREGEGKKKIAKSYLVPQTVKRSSGVAANLLWDHSGYVFGADDKGKPERVREQH
                     LAFVEAIRSRFPASNDAGIQAVLSFLERNNFEKVFLHSSWPEILETGANLTFRLEGDS
                     GPVCERPEVAAALSTKDDVDSDKASLCLVSGEEDVIERLHPSVKGVWGAQSSGANIVS
                     FNLDAFSSYGKSQGENAPVGKRAVFSYTTALNHLLAKDSRQRIQVGDASTVFWAAAPG
                     HPLEELFPGWFSEPPKDNPDLGTAAVAALLSAPKTGAGSFEADDTRFYVLGLAPNAAR
                     IAVRFWHVKTVGELAVNIRRHFDDLRIVHAPHEPETLSLFRLLVATAMLGKSENIPPN
                     FGGDVMRSVIEGSPYPQTLLSGTIRRIRAEHEVTYPRASIIKACLNRQSGKEELKVSL
                     DENNTNTAYRLGRLFAVLERIQERTNPNINATIRDRYYGAASGTPVTVLPTLLKLKNH
                     HLAKLDNKGEVINYERALGQIMDGITDFPAQLNLQDQGRFAIGYYHQRQAFFTKSEST
                     IKGE"
     gene            19510..20391
                     /gene="cas7c"
                     /locus_tag="PKZ49_01755"
     CDS             19510..20391
                     /gene="cas7c"
                     /locus_tag="PKZ49_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HND35315.1"
                     /translation="MALNNRYDFVLLFDVRDGNPNGDPDAGNLPRVDAETGRGLVTDV
                     SLKRKVRNFVGLTKEEQPPFEIYVKEKAILNNQNKRAYMGIGKAELLEGDDKKRKGGD
                     AVDEARQWMCKNFFDVRTFGAVMSTGINCGQVRGPVQLTFARSVDPIVAQEHSITRMA
                     VATEAEAEKQGGDNRTMGRKFTVPYGLYVAHGFVSAHLANQTGFNEDDLELLWQSLVN
                     MFEHDRSAARGEMATRGLYVFKHDSQLGNAPAYNLFERIQPKLKDGIVVPRSFEDYQV
                     NVNETSLPSGVTLERKA"
     gene            20394..20609
                     /locus_tag="PKZ49_01760"
     CDS             20394..20609
                     /locus_tag="PKZ49_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35316.1"
                     /translation="MKNANEIIFLVEEAPEGGYTARALGESIFTEADDLANLHQQVRD
                     AVHCHFDEGKAPEIIRLHFVREEVIQT"
     gene            20606..20866
                     /locus_tag="PKZ49_01765"
     CDS             20606..20866
                     /locus_tag="PKZ49_01765"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HND35317.1"
                     /translation="MKLPRNISGARLIKALNRLGYGITRQVGSHVRLECQLPKPHHLT
                     VPIHDPLRVGTLASILNEIARHHSLSKEEVILRLFGKINDHN"
     gene            20853..21479
                     /gene="cas4"
                     /locus_tag="PKZ49_01770"
     CDS             20853..21479
                     /gene="cas4"
                     /locus_tag="PKZ49_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HND35318.1"
                     /translation="MTTIEVDEPIMLSALQHYSYCPRQCALIHVEQAFDENVHTMRGN
                     TVHQRVDEPGSESFEGVRSEKALPIWSERLGLIGKCDIVEFYSNGRIYPVEYKHGKKR
                     AKAHDDIQLAAQAICLEEMTGKTVTHGAIYHHSSRRRREVVINELLRQQVEETVQAVR
                     ALLVSGKLPPPVNDVRCKECSLKEICQPEVLADKARVLDAQIHLFEVG"
     gene            21489..22523
                     /gene="cas1c"
                     /locus_tag="PKZ49_01775"
     CDS             21489..22523
                     /gene="cas1c"
                     /locus_tag="PKZ49_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1c"
                     /protein_id="HND35319.1"
                     /translation="MYQILNTLYIMTPNAYVHLDNDTVRIDVERQKKLQVPLHHLGTL
                     VCFGNIMISPALMHRCADDGIGLVLLDANGRFKARLEGAVNGNILLRQMQHRKAIEFP
                     FSLAIAKAVIAGKLRNCRQVLLRGAREATDEQDQGVLSNAAATLANSIRNLPTAADLD
                     VIRGIEGDAAKVYFSALPNLVRHNIREYFTMDGRTRRPPRDRFNALLSFLYSLVMNDC
                     RSAIESVGLDPQLGFLHSVRPGRAALALDLMEEFRSILADRLALTLINRSQITEHDLK
                     IREGGAVQLENDARKVVVAAYQEKKQEEVTHPLLNTKVAFGLLPQLQARFMARAIRGD
                     IDSYIPFLIK"
     gene            22526..22816
                     /gene="cas2"
                     /locus_tag="PKZ49_01780"
     CDS             22526..22816
                     /gene="cas2"
                     /locus_tag="PKZ49_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HND35320.1"
                     /translation="MLIIVTYDVSTETKEGRRRLRRVAKICEGIGQRVQKSVFECKVD
                     LMQYEELERRLLAEIEEKEDNLRLYRLTEPVALHVKEYGKFKAIDFDGTLVV"
     repeat_region   22997..24173
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=22997..23027
                     /rpt_unit_seq="gtagcgcccagcatccaagctgggcgaggat"
BASE COUNT         6848 a         4798 c         5958 g         6570 t
ORIGIN      
        1 gaatgttttt tttgtgttgc tgctcctcgg tatcgttggg ttcttactat ggaggctaag
       61 aaaaaggctt aaaacacgag tttttggttc gcggttggcg tttcgactgt tagtggtttt
      121 ttcattaatg gcaattctcc ctggggcatt ggtatacggg atctcagttc aatttctcgg
      181 gaagagtatc gaatcttggt tcgatgtcaa agttgatcaa gcgcttgaag gtggaatcaa
      241 tctaggccaa accatgctgg ataatttact ggaagagctg gaaaaaaaag cgaaggttac
      301 agcactagcg cttgctgatt cgccgtctca aactctttct tcattaaacc aacttttagc
      361 acattcctat atacaagaag cgactttgtt caatcaagat ggaaaagcca ttgcgttttc
      421 aagtcttgat gatgtcgcgt tatttccaga gatacctaat gcaactgcta tgcgacatat
      481 tagaatgcaa aagagctata gcgcagttga gtcaattcct aataaagggt tgtttttacg
      541 ggttctcgtt cctgttaatg tgctgagtct ggaagacgat attcgcgtat tgcaagtgtt
      601 gcagcaagtg ccttctggcc ttgccgaaaa tgctgagatc gttcagaccg ggtatcgaaa
      661 ttaccaggag ttggtactat caaaacaggg gattacgcga ctatatagtg tgacattaac
      721 atttgcactg ctgttagcgc ttttttcagc gctagcgagc gcttctctta tcagtgaaaa
      781 attaagtgcg ccgctgggta tgctcgcaga agggacacgc gccgttgctc agggtgattt
      841 cagcaggcga catcaggttt ttagcaatga tgagctgggt attttaacgg aatcgtttaa
      901 cttaatgacg gagcaattag atctggctcg cgttaatgcg caacaaagtc agcatgagat
      961 tgaaagtgcg cgtgcttatt tagaaagtat tctggcgaat ctttcttctg gggtgatggt
     1021 atttgatgac aaactaaaaa taagaacagt caatcgcagc gcggagcaaa tcttacaaat
     1081 tccattaatt tatcttgaag gcctggcgat cgatgagtgt gttgagaaag agcctaagtt
     1141 tcatctttta gcatcagaaa ttcagaatgg atttgggtcc actcaaagtg gagaatggca
     1201 aaggcaggtt ttgcatttta gtgaaagtaa agaccaagtt ttgctgctac gtgggacgcg
     1261 attgcctgaa tcttcaggcg gtggtggaat cgtcgtgttt gacgatatta caggattatt
     1321 gcaggtgcag cgtaccgtgg cctgggggga aattgcgcgt cgactggcgc acgaaataaa
     1381 aaatccactg accccgatcc aactttctgc agagcggatt cagcataggt taatggataa
     1441 attaagcgga caggatgcta aaattttaca gcgttcgact gaaaccattg tcaaccaagt
     1501 tgaagccttg aaaaagatgg taaatgagtt tagtgaatat gcgcgtattc ctgaattgga
     1561 aatggttcag attgatttta atcggttaat tcatgaggtt ttggctttgt acgaaacctc
     1621 taatgctaag gccgagaatt cgcagtgccc ggaaataaaa ttagcgttat cctctgatct
     1681 tttgctaatt cggggtgacc cggcaagatt gcgacaagtg attcataact tactacagaa
     1741 tgctcaagat gcgctagcag atactaagaa tggtacgatt acggtgagta ctgatatgat
     1801 ttcaggaaat gttaggttgt gcattatcga taatggaaag ggttttgccg aagaaattaa
     1861 aacactggct tttgagcctt acgtaaccac taagccaaga ggtacaggac ttggattgcc
     1921 aattgttaaa aaaattgttg atgaacataa tggtgcaatc caaattgaaa atacccaacc
     1981 ccatggagca aaagttacaa ttacgttgcc ggcgattaac gctgggcaag tggcatcgac
     2041 aatgaaccga ttggcctgat agtggtaaag cataatgggg accaaggaag cagattgaac
     2101 tcggatacga aaggagtgat atatggccaa caatactatt ctagttgttg atgatgaaat
     2161 tggcatacga gaattattgt ctgagattct aagtgatgaa ggctaccgag tagtattggc
     2221 tgaaaatgcg gagcaggcgc gcgtttgtcg taatcagagt cggcccgatt tagtattact
     2281 cgacatatgg atgcccgata cggatggtat taccttactt aaggactggg caaatagtgg
     2341 tgtattgact atgccagttg tcatgatgtc aggacatgga acgattgata ctgctgttga
     2401 agcaacgcgc atcggtgcgg ttgattattt agaaaaaccg attccccttc agaaattatt
     2461 gagcacagtc aagcaagcgc ttcggggagg gcaaagtaag ccagcgttaa cgatttcttt
     2521 ggccaattta ggcaaggggc ctcaaatcgt tgaattgaag aagaaactcg agcaaattgt
     2581 caatctggtg tcgccggttt tgttggtggg cgaatccggt gtaggggttg agcattgtgc
     2641 tcgcttcttg catcgcgtta atactccctg ggttgagccg gagcaacttt cttttttggc
     2701 agataacccc gtgggaattt tagagcaagt gaaaaatggg ctgctctttt ttagagagat
     2761 cggtggtctt aataagctag aacagaaagg tttgctactt ttgcttggaa agttggaaaa
     2821 atttaatgtt cggttggttt gtgcaacgac gcaacccttg gcagagctaa ctgcgcaagg
     2881 tacctacaat tcaaaattgt atgagatact cagtaatcta acgattaacg tgccctcatt
     2941 aaggacacat cgtgaggata ttccagagct tgccaatcaa cttctttcaa cgagcattga
     3001 aacaggtgaa gttccttttg tccattttag tacagcagcc cttaacacat tgcgtaatta
     3061 tgactggcct ggaaatttgg cacagttgaa gagtgttgtg cattctttgg cgctaacatg
     3121 tactggcagt gaaattagcg ctgaggaagt tcaacaagta cttacgcccc cattatcgcc
     3181 caccatgtct gctacaccag atataccgct caatgtttcg ttgcgagaag cgcgggattt
     3241 gtttgagaaa aattattttg agaagctcat tgatcaggaa agtggcaata tgacacgtgt
     3301 tgcagaaagg gcaggtcttg aacggacgca tctttatcgg aagattaaaa tgcttggaat
     3361 taaactacgc ggtcatggtg gttaagtttc aacatcacat cgtggaatcg atattgtagc
     3421 gaatcctaag ttagtagata ataacagttt ttgccgactc gccttgtcaa ttttttgaca
     3481 gggaaggttt cgttttgatc attattgggg agctagtata taaatgggag catttgggga
     3541 ttttaaagcg ccagtattca agaatctaac aagcgcaatt cgtgcattgg cgatggatgc
     3601 agtacaaaag gctaattctg gccatccagg catgccaatg ggtatggcag aaattgctga
     3661 ggtgctttgg attcatcata tgcgccataa tcctagtaat ccaaaatggg ctgatcgtga
     3721 tcgttttgta ttgtctaatg gccatggctc aatgctgatt tacgctttgc tccacttaac
     3781 tggatatgat gtgtcaattg aagatataaa aaatttcaga caacttcatt ctaaaacacc
     3841 aggacatcct gagtatggtt atacaccagg ggtggaaaca acgactggcc cactcgggca
     3901 aggaatcacc aatgcggtgg gtatggcact cgcagaaaaa attcttgcaa atgaattcaa
     3961 tcgacccggt ttcgatattg taaatcacta tacgtatgta tttttaggtg atggctgtct
     4021 catggaagga atctctcatg aagcctgttc attagcagga acgcttggtt taggtaagct
     4081 catttgtttc tatgacgaca acggaatctc tatcgatggc catgttgaag gctggtttac
     4141 tgatgataca cctaagcgct ttgaggccta tggttggcat gtgatcccga atgtgaatgg
     4201 tcacgatcca gaagcaattg aagccgcaat cgaagaggct aagaagaaac aagataaacc
     4261 atcgatgatt tgttgcaaaa cggttattgg catgggttca ccgaataaag ccaatacgca
     4321 tgcggtgcat ggagcggcgc ttggtgatga agagattgct gcaacaagac cgcatatagg
     4381 ctggcaccat ctgccctttg aagtgcctaa agaagtctat gatgcatggg atgcacgcac
     4441 aaaaggtaag aaacttgaaa gtgagtggaa tcaaaaattt tctgaatata gccagaaata
     4501 tcctaaagag gctgctgagt ttacgcgccg ggtcaatggt gatttaccca aagattggtc
     4561 agcgtatgtt gacggtgtag tgggaagagt gaatgcaaaa ggtgaaacga ttgctacacg
     4621 taaagcttcc caaaacgcca ttgaaggctt ggcgcctgcg cttcctgaat tactgggggg
     4681 atcagcagat ttagctggct ctaaccttac cttgtggtct ggttcaaaag gggtaagtag
     4741 cgaatcaggc ggtaattaca tttattatgg tgtaagggaa tttggaatga gcgcaatcat
     4801 gaatggattg tcgctgcatg gtggttttat tccttatggc ggcactttcc tcatgttttc
     4861 agaatatgca cgaaatgcat tacgaatggc agcgttaatg aaaattcgga gtatttttgt
     4921 gtttactcat gattcaattg ggttaggtga ggatggtcca acgcatcaac cggtagaaca
     4981 aacagcaacg ttacgtttaa ttccaaatat ggatgtttgg agaccttgtg acacagtgga
     5041 atcaacagcc gcctgggcac gtgcgattga acggaagaat ggtcctacaa cgctgatttt
     5101 tagtcgccaa aatttacctt tccaaaaacg agaggctgat gttatcaagc taattgacaa
     5161 gggtggttat attttatctg aagcagcgga tggcaagcca cgagcaataa taattgcgac
     5221 tggatctgaa gtggggcttg caatggcagc acaaaaaacg cttgctgatg aaggaattca
     5281 tgtacgagtg atttcgatgc cgtgtactaa tttatttgat cgacaagaca aggcgtatca
     5341 agaggcggtc ttgcctaaag gaatcaagcg tgttgcggtc gaggcaggtg taactgatta
     5401 ttggcgtaaa tatgtaggcc ttgaaggtga aattattggc attgatacgt ttggtgaatc
     5461 tgcgcccgct gaagttttgt ttaagcattt cggtgttacg acagagaatg ttgtaaatgc
     5521 aataaaaaga ataatttaag cacgatgagt tttctcaaga tgctgtacta gttttatttt
     5581 agtaattgat ttgaggagta gaaaaattat ggcaattaaa gttggaatca atggttttgg
     5641 tcgtatcggt cgtatggttt ttagagcatc gatagaaaaa tttagcgata ttgatgtagt
     5701 tgcaattaat gatttgctgg agccagatta tttggcttat atgcttacgc atgattctgt
     5761 tcatggtcga ttcagcggca ccgttgcaat tgaaggcaat acattggtcg tcaatggaaa
     5821 gcgcatacgt ctcacagcca ttaaagatcc ggctgaactt aaatggaacg aagtgggtgc
     5881 agatatcgtt gtggaagcaa ccggtttgtt cttaacaaaa gaaacctgtg agaaacatct
     5941 gacagctgga gcaaaaaaag taattatgac agcgccttct aaagatgata cgccgatgtt
     6001 tgtttatggg gttaacgata aagcctatac gggtcaaacc attgtttcaa atgcatcttg
     6061 tacgacaaat tgcttagcac caattgctaa agtattaaat gatacgtggg gcattaaacg
     6121 tggtttgatg acaacagtgc atgctgctac tgctactcag aaaacagttg atggcccatc
     6181 taacaaagat tggcgtggtg gccgtggtat tcttgagaat atcattccat cttcaacagg
     6241 tgctgcaaaa gcggtaggtg tcgttattcc agaattaaat aaaaaattga ctggtatggc
     6301 atttcgcgta ccgacatctg atgtatcggt tgtcgatttg acggttgagc tcaacaaaga
     6361 cgcaacttat gacgagattt gtagcgtaat gaaatctact tctcagggga ccatgaaagg
     6421 cgtactggga tataccgatc aaaaagttgt ttcaactgat tttcgtggtg aaagctgcac
     6481 ttctgttttt gatgctgaag caggtatttc tctggataaa aacttcgtga aagtagtcgc
     6541 ttggtacgat aacgaatggg gttattcttg caaagtgtta gagatggtta aagtcattgc
     6601 aagcaagtaa agcgtcaaag ctggtcagtt aatgattgat ttgctgacca gaatcttcta
     6661 attagtttta atggagttaa cgtgtcagtc attaaactcg tcgatcttga tttgaaaggt
     6721 aaacggattt ttatccgtgc tgacctaaat gttcccatcg cagatggaaa ggtaacatcg
     6781 gatgccagga ttacagcatc catggcgact atcaaacact gcctagagcg gggagggaaa
     6841 gtaatggtta cctctcatct aggaaggcca gaagagggag tatggagtga agaaaattca
     6901 ctaaaaccag ttgcagacaa tatcgctgca cgccttaaca aaccggttcg acttattaaa
     6961 gactgggtga atggcgggtt tgatgtggct gagggcgagt tagtcgtact agaaaattgc
     7021 cggatcaata agggtgaaaa aaagaatgtc gatgaaacgg cacaaaaata tgctagcttg
     7081 tgcgatgttt tcgtaatgga tgcgtttggt acggcacacc gggctgaagc atcaacacat
     7141 ggcgttgcta aatatgcacc ggttgcatgt gctggaattt tattaacaga agagcttgat
     7201 gcgttaacca aagcattgga taagccagca aggccgatgg tggctattgt tggtggttca
     7261 aaagtttcaa caaaacttac tgtattggaa tcactctcgg aaaaagttga tcaactcgtc
     7321 gttggggggg ggattgcaaa tacctttttg aaggcggctg gtaagaatat cggtaaatca
     7381 ttgtgtgaag ccgacttggt tcctacagct aaggtattga tggataaaat gcagcaacgg
     7441 aatgcaacga ttcccattgc ggtcgatgtc gtggttggta aaaaatttga tgcaaatgag
     7501 cctgctatca ctaaatcggc tgataccgtt gcggatgatg acatgatttt tgatattggt
     7561 cctaatagtg cgcaagagtt ggttgatatc atcatgaaag caggcacagt tgtttggaat
     7621 ggcccagttg gtgtatttga gttcgatcaa tttggtggtg gtactcgacg aattgcgcaa
     7681 gccattgcag aaacacaagc atttacactg gctggcggcg gagacacgat tgccgcaatt
     7741 caaaaatacg atatttacga taaggtttca tatatttcaa cagccggggg cgctttccta
     7801 gagtttctag aagggaagaa acttccagcc gtagaaattc tggagtcaag agcaaagcaa
     7861 tagtttactt aggctgcctc ggtcatgttt agaagaacca aaatagttgc tacactgggt
     7921 ccagcttgta gcgatccagc tgtactcaaa cgcatgattg tggcaggagt tgatgttgta
     7981 agaatcaatt tctcccatgg gacgagagag caacatatcg cttttgctga attgacacgc
     8041 tccttagcgc attctgtggg ttgtactgtc ggggttttag ctgacttaca aggccctaaa
     8101 attcgaatag gaaaatttga acaaggtaaa atctggctca atgtgggcga cgaatttatc
     8161 ctggatgcta actgtcagct aggaaataaa gagagaatcg gtctcgacta taaagaatta
     8221 cccaatgacg ttgaagaagg tgcgatccta ttattggacg atgggcgcat tgttttaagc
     8281 gtaaacagag tcaaaggaag tgaaatattc tgtgaagtcg tacaaggcgg gccactttcg
     8341 aacaataaag gcatcaatcg caaaggcggg gggcttagcg cacctgcgct aacgagtaaa
     8401 gatattgaag atattaagat tgcagcagat cttaaagccg attatctagc cgtatccttt
     8461 ccccgttctg gagaagatat tcgcctagca cgttcgttga tgcaggaagc aggtggaaat
     8521 agcttaatca tggcaaagat tgaacgaact gaagctattc ttgcattgga tgacatcatg
     8581 gaagcatcgg atgcattaat ggtcgctcgt ggtgatctag ccgtagaagt aggggatgca
     8641 gcagtacccg cactgcagaa gcgcatgatc cgttcggcgc gtgcaaataa tcgattggtt
     8701 gttactgcaa cacaaatgat ggaatcgatg attacaaatc cgattccaac cagggcggaa
     8761 gtgtctgatg tagccaatgc cgttcttgac ggaactgatg cagtaatgct atccgctgag
     8821 tctgctgcag gccagtttcc tgtagaatca gtagaagcaa tggccagagt ttgtctcgaa
     8881 gcagagaaag aatatacggt aaatcaaagt ttacgacgct cgcccgatac gcctcctgtg
     8941 agtatagaag aggcaatagc gcgtgcaacg atgtatacag caggtaatct caaaattaga
     9001 gcgattgctg cgttaacaca aagtggaaaa acagcattgt ttatgtcgcg gagaagctcg
     9061 aaggtcccga tatttgcgtt gagtccccag aaagatacgc tgggtaaaat gacgcttttt
     9121 agaggggtct atcctgtcga atttggtgaa ggattatatg atccagaagt gatccttgat
     9181 ctggcagaag atgagttatt caaacgtggc gccgttcgtg atggagatgt aattatcatg
     9241 acgattggag agcctgttgg taaagctggg ggcaccaata cgatgaaaat tgttcgagtg
     9301 ggcgattgta ggaagccgct gaatatcgat aattttgagg ctaatatctg atcaatcaat
     9361 agcgatttaa tcaccgagaa gactcattta agattttatt ttgatatttt tttaattagg
     9421 taatatttag gaaggagaat atagatggca cttgtttcat tgcgtcaatt gttggatcat
     9481 gctgcagaaa atggctatgg tttaccagca tttaatgtaa ataatttaga acaaattcaa
     9541 gcgattatgc aggctgctga cgaatgtaat agccctgtaa ttatgcaagg ttctgctggc
     9601 gcaagaaaat atgcaggtga agcttttctg cgccatttga ttgcggcggc tgtagaagct
     9661 tatcctcata ttcctatcgt gatgcatcaa gaccatggcg cttcttcagc ggtatgtgtc
     9721 aatgcaattc gcagtggttt ttccagcgtg atgatggacg gatcattaga agaagatgct
     9781 aaaacacctt cttcttatga atataatgtt gatgttactt ctcgagtcgt agcgatcgcc
     9841 catgcagtcg gtgtatcagt tgaaggggaa ctgggttgcc taggttcact cgaatcagga
     9901 atgggtgaag cagaggatgg ccacggtgca gaaggggtgt taactcatga tcagttatta
     9961 actgatccag agcaagcagc tgattttgtt aagaagactg gcgtagacgc actcgctatt
    10021 gcaattggaa cctcacatgg cgcttataaa tttacacgta agccaactgg cgatattctt
    10081 gcgattgaac ggattaaaga aattcataaa cggataccaa atacgcattt ggtaatgcat
    10141 ggatcgagct ctgttcctca agaatggctt gaaatcattc gtgagtatgg tggagaaatg
    10201 aaagagacct atggtgttcc cgttgaagaa attcaagaag ggattaaata tggggtcaga
    10261 aaagtcaata tcgatactga tattcgtttg gcgatgacag gtgctattcg ccgccatctt
    10321 gctaaaaaca agagcgagtt tgatccacgc aaattcctaa aagatgcgac ggctgcggct
    10381 aaagatatat gtaaggcacg ttttgaagca tttggatgtg ccggtcaagc aagtaaaatc
    10441 aaacctatca tgcttgacaa catggcaacg aaatatgcaa aaggtgaact aaaagcgatt
    10501 attaaataat caaaagaaga tataaatgtg gttgattact gagcaatctt taatcaacca
    10561 catactaacc cctaccctca attattgacc atttctctga aatggtgaca tagtaagaaa
    10621 ttccttccag ttgtttttta agtctgcttt catttgcgta gttatatacg ctttccatcc
    10681 gtgaataacg cctattggtc gtgctagctt tttttgcttt tgtgatgaga gaatggattc
    10741 aagttgctga tcaatcatat agttatcatt gattgatccc agcgcacctt ggagtatagc
    10801 gagtaattga acgtagtatt tgaaacattc ctgcttcgca tagagtgagt aaaaaaattc
    10861 tgctgcatag cgctgtttct tgatttggat tcgtaaagca tgtaaggcgg caggtttcat
    10921 tttctccagc ttctctttat ttttggtgat ggcagaatgc tggtcaagta agtaggatcg
    10981 ggaaaacgat ccaatcgata tactcgattc tagcttgatg tcgtgacgca agtcataaat
    11041 ccataaattg agttttaaaa taaacgctag gtagcgctta gattggagat ttgtttgcac
    11101 ttgttgggta tatttactgc gttgcttttg tcccaacttt tgaataactt taattggctc
    11161 aacatcaggg caactagaga tgattggcgg taggatttca ttggcaaaaa cataccaatt
    11221 tctaactgga cttagcaagt gcattaacca cttaagctcc tgttcaaaaa aggtgatcga
    11281 ttgatgctgt tcggaaagta gtggcttaaa aataataaaa gcggagcgaa gtcggcgaat
    11341 cgcaacacgc atttggtgga gatattcggg atcttcacta aataagacgc cattctcatt
    11401 cttagttaat tgcaagaggc aactcgaaag aatcttactt aaagcatcta agaatggagt
    11461 cttggtattt atttcaatgg atgctgcttt ggtagggggc gtacgatcgc ctataaacag
    11521 tttgtaaccc ctttctgctt tattcacatt ttcaagccgg attggaaaat cggtgagctg
    11581 tgcaatgtct aaggcgagct ggaacagctg ggcagaattg cccgatttta gttcgagctc
    11641 gatttcatgg atggaatcat tgagatgatc cgcagtaatt tttccatggt ctaagcaaaa
    11701 ttcaataata ctgccatctt tggtttgtaa ggtatgggta tcacgcgtaa attgtgtaat
    11761 aaagattggc tttaatgttc taatgatttt gcgctgctga gcaagggtga taacacccgt
    11821 ctgttgtaag tgatctaaat caggttgagc cttatttaag ggaatctcag attcattgtg
    11881 ttgatgtaga cctgctgtaa tatttccacc tgatttaatg gtttgaatcc attgtttgcc
    11941 aatgcggcgt atgcgcaacg aacagcgctg atttcttagc gataaatcgg gtgtatcgta
    12001 ataaatacta taaagtttct gttttttcgg gggagcgatt gataatgaac tcaggatagg
    12061 taattgtgct atcgctttga tatgacccgg aggaatggtg agtttaagtt cgatttctcg
    12121 tgtcatagga ggctttggag cttaagtttg ttgagccgcc acgagcttcg cgagcaatgc
    12181 ttcttgcgca cagaattgtt tgccgcgatg tgaaactttt cgcttgtaat agccattgca
    12241 atccatttcc caagcttgaa cattatcttt taaataaggt tttaatccct cagtgataat
    12301 gcgcttcttg agacgtgaat ccagaaccgg gaagcaaatt tcgattcgcc tgaaaaaatt
    12361 tcgatccatc caatctgcgc tcgatagata aacatgatgc tggttattat cacgaaaata
    12421 aaaaatacgg gagtgctcga ggaaacgacc aatcactgaa cgtaccttga tatttgtgga
    12481 taagccaggt acaccagggc ggagcgcaca agcccccctt acgattaagt ggataattac
    12541 gcctgcatta gaggctgcat aaagcgctcg aataattcca ggttccagta atgcattcat
    12601 tttggcaatg atatgagccg gtttacctgc ttttgcattt tcagcttctc ggttaatcgc
    12661 atccatgatt tgatgatgca gactaaatgg tgattgccat aagtgtttta acttgctggc
    12721 tttaccaagg ctcgtgagct gaaaaaacac tgcattaaca tcagcacaaa tctcttcgtg
    12781 acaggtaatc aaaccaaagt cggtataaag ctttgcggtg ccggtatgat agttgcccgt
    12841 acttaaatga acatagcgtc gcaatctgcc ttcctcacga cgtacgatca tcgccatttt
    12901 agaatgagtt ttatggccaa ctacaccata aaccacatgg gcaccgactt cttcaaggcg
    12961 gccggcccaa ttgatattgg cctcctcatc aaatcgtgct aagagttcca cgacgacagt
    13021 aacttctttg cctttgcttg cagcatgaat cagtgcttcc ataatggttg aatcagagcc
    13081 agtccggtag acagtttgct tgattgcaac gacatcggga tcgcttgaag cttgctgtat
    13141 gaaatgaata accggttgaa aagactgata aggatgatgc aacaagatat cgtttttgcg
    13201 gatttgatgg aaaatatcgt tatttttttt cttttgtgat gaaaagggta ggccaggtat
    13261 gaacaatgga aatttaagtt gggggcggtc aatttgatcg ggaacgcgca ttagccttac
    13321 gagattaacc ggcccactca cttggtacag atcatcttgt gtgaggctta attgatcgag
    13381 taggaaggta atcatatggg gcggacaact gtcggccact tcaagtctaa ccgcatcgcc
    13441 caaatgtcgc tgaggaagct cgccctgaag tgcaatacgc agattcttga tttcttcttc
    13501 atcaacgaaa agatcactgt tacgagttgc acgaaattga aaacaaccta tcgcctgcat
    13561 acctgagaaa agctcaccca catgagcatg caaaatcgat gatagaaata taaaactatg
    13621 ctcttctgta ctgacatcac tgggcagcct aatgatgcga ggtagaatgc ggggggcttg
    13681 aacaatcgcg atacccgaat tacggccaaa cgcatcttta ccttccagtt ccacgataaa
    13741 gtttaggctt ttgttcaata cgcgtggaaa gggatgagag ggatcaagac caatgggcgt
    13801 taacatcggt attaattcac ggaagaaata tgctttaatc cagtctcgct gtacttcgtt
    13861 ccagcttgct ctgcggataa aacgaatgcc ctgctgctct agctcaggta ggatactttc
    13921 attgagcaat tgatactgtt gcgccacgag ttgatgaatc tgctctgaaa ctcgtttaaa
    13981 ggcttgtcgt ggcatcattc catctagacc aaaaccgggc gcatctaatt taatctgttc
    14041 ctttagtcca gcgacacgga cctcaaagaa ttcatcaaga ttactactga caatgcacaa
    14101 aaaacgtagt cgttcgagta acggcatctc cttattctct gcctgggcaa gaacacgacg
    14161 attaaactcg atctgactga gttctcggtt taaaaactga actgaattag atagatcatt
    14221 cattatgggt gcactacttt ttcaacggta tccacagcat gggttggtgc ctagaaaaat
    14281 acagtagatt accggatgat tgtgatgttt ttatgtattg tcactgggtg caacataacc
    14341 ggtgggtttg tctacgcctt caccaaaaaa ataagcttcc atttgtcccg ctagatattt
    14401 tcgtgctttg atatcagcca agttcaagcg atattcattc accagcaaag tttggtgctt
    14461 gatccattga ccccaagctt gttttgacac attctcaaaa atacgctttc cgagttctcc
    14521 ggggtaagta ggggcatcga gcccttctgc ttcatgtcct agtttgatgc atttaacgat
    14581 tcttgccata gttttaatag ttaaaatgag aaggtagggt taaaaaactt ctttaacaag
    14641 cattttattg tactaataac tgcgtggaga agctaagaat tactgtgatg gcggcgttct
    14701 atttcgacta aacaaataat attagaggtt gcaataaaat aaatctgcat tcattactga
    14761 aattaaacga gagaaaactg attttttagt agttctatga ggataaattt atgtaaagat
    14821 cttaggtaat acactattcg taatggttag tcgcttatta ttatggcaga taatgtacaa
    14881 aatcagagca ttgcgcttgc tcacgtaagg aaaaatgaag aaggtaaatg gaaagaacac
    14941 ttgcttcatg aacatctaga gagagtttca tatcttgcgg gtcaatctgc aactacgttt
    15001 ggtagtgaag attgggcaag gatagctgga ctctggcatg atttaggtaa ataccgcgag
    15061 aaatttcagc aatacatcaa atcggcgagc ggttacgatg cagaagcgca tatcgaagac
    15121 gcaacagggc gagtcgatca ttccacagct ggtgctattc atgccatcga aaaactcgga
    15181 ttgcaagggc gtattctagc ttatctgatt gccgggcatc atgccggttt gccggattgg
    15241 aacaatgcgg aaggtagtca atcggtgcta tttcagcgta tacaaaacgg taaacaaaat
    15301 ggctatttgc aagaggcgct tgatgcgatg ccaccggctg aaattctcaa tcaatcccgt
    15361 ccacaatctc tgccgcccaa tggatcgctg gcattgtggc tcagaatgct gttttcctgc
    15421 ttggtggatg cggattttct ggatacggaa gctttcatgg atgataaaag gagcggcttg
    15481 cgctcctggt atccttcgat agtagcgctg aaaagcgcat tcgatcaaca catggccgat
    15541 aaagctgcca acgctaaaga tagcgatgtc aatcgcattc gcgctgaagt actgcggcag
    15601 tgccgcgaaa aagccaaatt accttctggc ttattttcac tgaccgtacc gacaggtgga
    15661 ggaaaaacat tgtcgagcat ggctttttca ctcaatcacg ctgttcatca cggtaaggat
    15721 cgggtgattt atgtcattcc ttacaccagc attctggagc aaacagccga aatctttcgg
    15781 agtattttcg gtaacgataa tgtgatcgag catcacagca atcttgatcc cgatcgtgaa
    15841 gatgcgcgct cccggttagc aacagaaaat tgggatgccc ctattattgt taccaccaat
    15901 gtgcagtttt ttgaatcgtt atttgcggca aaaaccagcc gttgccgcaa gctgcacaat
    15961 atcgtcaaca gcatagtggt attagacgaa gcacaactgc tgccaccgga atttcttgcg
    16021 ccgattttac atgtgataca ggatttaaca aatagttaca aagtcagttt cgtcctatcc
    16081 acggcaacgc aacccgcttt ctcgccgcgc ccaaaattta ctggattaaa gaatgtatgc
    16141 gaattgatgg ataatccgga gcggctctat gccgacctga agcgggtaaa aaccgaattg
    16201 ccggatgatt ttgccagtcc tcgcacttgg gaatccatcg caagagaatt gcagcaatat
    16261 gagtcggtgc tttgtatcgt gaactcgcga agggattgtc gtgaattaca cagcttaatg
    16321 ccgaaagata cgattcattt gtcggcattg atgtgtggac agcatcgaag ccaagttatc
    16381 accggaatca aacagcgcct gaaagatggc atactaacgc gcgtgatcag tacccaactg
    16441 gtcgaagctg gagtcgacat ggattttcca gtggtttatc gggcattggc agggctggat
    16501 gcgatagcac aggcagcagg ccgctgcaac cgcgagggaa cactatccga gatgggcaaa
    16561 gtagtggtat ttgtgcctcc caaacctgct gctccaggtt tattacgcaa agcacaacaa
    16621 agcgggcagg aaatcatgcg attatctaaa ggtgacccgc tcactcgaga acgttttgaa
    16681 gcctacttcc ggcactatta tgaaagtctg aacagtcttg ataaagcaaa cattgttagt
    16741 ttattggatt tacataacca agccgaagta agaaaagcag aatttaactt ccgtaccgcc
    16801 gctagcaatt tccgcctgat cgatgaagaa ggccaaacgg ctgtcatcgt gcgctatggc
    16861 gaaagtacaa ggctgattga tgcgctcgag aaaagtcaaa atatgcaacc gcatgaacgc
    16921 cgaggcatat tacgcaaatt gcaacgttat acagtcaaca ttcgcgaaca tgaatgcaga
    16981 aagctattgg agaatcgaga aattcaggaa attttccccg gcatacacat acaaaacaca
    17041 gatttacttt accattcaca gctaggattg ctgatgagta aagatgttgt gtttgatccg
    17101 acacagagcg tggtctaaat aggaggacaa atgaaaatat attgccttga agtccgcggt
    17161 gatttcgcct gctttacccg accggaaatg aaagtcgagc gtgtgagtta cgatgtaatg
    17221 acgccttcgt ccgctcgcgc ggtattcgaa tccattctat ggaaacccgc gattcgctgg
    17281 catatcgata aaattgaggt actcaaaccg attcgctggg tttctatccg gcgcaatgaa
    17341 gtcggtgcgg taatttcagt gcgcaatgct caggaagcga tgaacaaagg tacgggctgt
    17401 cttgggttaa atatcgaaga tgaacgccag cagcgtgctg gattattttt acgcgacgtg
    17461 gcttatcgca ttcatgcgca tttcgagttg caaccagatg ccggagaaaa taatacgcca
    17521 gccaagtttt tggatatgtt cgaacgccgt gctgagaaag ggcaatgcgt taatcaaccc
    17581 tatcttggtt gccgggagtt tgcttgcgat ttccggctga ttgatccaat gctagaacaa
    17641 tcgaatcctg taaaggaaac gcgtgacttg ggttggatgc tgtacgacat ggactacagc
    17701 aatgctgccg atccgcaacc gcgatttttc cgcgctacac tggagaacgg catacttaat
    17761 gtgccagttt ggaatagcca ggaggtgcga ggatgattct gcaagcactc aatgaatatt
    17821 atcagcgcaa aacccggttg ccggatagta acctggcgcc gcccggtttc gagcataaac
    17881 caattccctt tcttatcgta ctgacagtgg atggccactt tgctggtttg gatgacacgc
    17941 gggaaggtga gggtaagaaa aaaatcgcaa aatcctatct cgtgccgcag actgtcaagc
    18001 gatcttcggg tgtcgcggcc aatttgttat gggatcactc cggttatgtt tttggagccg
    18061 atgacaaagg aaagccggaa cgtgttcgtg aacagcatct ggcattcgtt gaagcaattc
    18121 gctcccgctt ccccgcttca aatgatgcag gtattcaggc ggtattgtct tttcttgagc
    18181 gtaacaactt tgaaaaagtt ttcttacatt cttcatggcc ggaaattctg gaaaccggag
    18241 ccaatctgac attccggctg gaaggtgatt ctgggccagt atgcgaacgt ccggaagtgg
    18301 cggctgcgct atcgacgaag gacgatgtcg actctgataa agcctcactt tgtctggttt
    18361 caggcgaaga agatgtcatt gagcgattgc atccttccgt caaaggcgta tggggcgcgc
    18421 aaagttccgg tgcaaatatt gtttcgttca atctggatgc tttcagttcg tatggtaaat
    18481 cacagggtga gaatgcaccg gtcggaaaac gtgctgtctt ctcctacacc accgcgctca
    18541 atcatttatt ggcaaaagat tcgagacagc gtatccaggt gggcgatgca tcgacggttt
    18601 tctgggcagc agcaccgggg catccgctgg aggaattgtt tcccggatgg tttagtgagc
    18661 cccccaaaga caatcccgat ctcggcaccg cagcagttgc tgctttattg agtgcgccaa
    18721 aaaccggtgc aggcagcttt gaagccgatg acacccggtt ttatgtgcta ggtcttgcgc
    18781 ctaatgctgc ccgtatcgct gtacgcttct ggcatgtcaa aaccgtgggc gagctggctg
    18841 tcaatattcg ccgtcacttc gatgatctgc gcatcgttca tgcaccgcac gaaccggaaa
    18901 cactgtcgct gttccgcctg ctggtcgcaa cagcgatgct aggcaaatca gaaaacattc
    18961 cgcccaattt cggcggcgat gttatgcgca gcgtgattga agggtcgccc tatccgcaga
    19021 cgctgctttc cgggacaatt cgccggattc gagccgaaca tgaagtcact tacccgcgcg
    19081 cttcgatcat caaagcgtgc cttaatcgtc aatcaggcaa ggaggaactc aaagtgtcac
    19141 tggacgaaaa caacacgaat accgcttacc ggcttggccg gttgtttgcc gtactggagc
    19201 gtattcagga acggacaaat cccaacatta atgcaacgat ccgtgatcgc tattacggag
    19261 cggcttcagg tactccagtc actgttttgc caacgctgct taagcttaag aatcaccatc
    19321 tagctaaact ggataataaa ggtgaagtca tcaattatga aagagcgcta ggacagatca
    19381 tggatggtat taccgatttt ccagcacagc tcaatttgca agaccaaggc cgttttgcca
    19441 ttggctatta ccatcagcgg caagcttttt ttaccaaatc cgaatctaca atcaagggag
    19501 aataatcgca tggcacttaa caaccgttac gatttcgttt tgttgtttga cgtaagagat
    19561 ggtaatccca atggcgatcc ggacgcaggc aatttgccgc gtgtagatgc ggaaaccggg
    19621 cgtggattag taaccgacgt gagcctaaag cgtaaagtac gcaattttgt tggtttgaca
    19681 aaggaagagc agccgccatt cgaaatctat gttaaagaga aagcaatcct caataatcag
    19741 aataaacgcg cctatatggg tatcggcaaa gctgaacttc ttgaaggtga tgataagaag
    19801 cgtaagggtg gcgatgctgt ggatgaagca agacaatgga tgtgcaaaaa tttctttgat
    19861 gtacgtacct ttggtgccgt catgtcgacg ggtatcaact gcggacaagt ccgcggcccc
    19921 gttcaactaa cctttgcccg ttccgtcgat ccgattgtgg cgcaagaaca ctccatcacc
    19981 cgcatggcag ttgctaccga agcggaagcg gagaagcaag gcggcgacaa tcgcacgatg
    20041 gggcgtaaat tcaccgtgcc atacggcctc tatgtcgcac acggtttcgt ttcggcgcat
    20101 cttgccaatc agaccggatt caacgaagat gatctggaat tactctggca atcgttggtg
    20161 aatatgttcg agcatgaccg ttctgctgcg cggggtgaaa tggctacacg cggtttatat
    20221 gtttttaaac atgacagtca attgggcaat gcaccagcct acaatctctt tgagcgtatc
    20281 cagcctaaac tcaaggatgg tattgtcgtg ccgcgctcat tcgaggatta tcaagttaat
    20341 gttaatgaaa ccagtcttcc atccggtgta accttggaaa gaaaggcata acgatgaaga
    20401 acgctaatga aattattttt ctggtcgaag aagcgcctga aggtggctat accgcccgtg
    20461 cactgggaga atccattttt accgaagcgg atgatcttgc caatctgcat caacaggttc
    20521 gtgatgccgt gcattgtcat ttcgatgaag gaaaagcgcc tgagataatt cgcttgcact
    20581 tcgtgcgcga ggaagtcatt cagacatgaa attgccgcgg aatatatcag gagcacgctt
    20641 aatcaaggcg ctgaaccggc tcgggtacgg gattacccgg caggttggca gtcacgtccg
    20701 gctggaatgt cagctcccca aaccccatca tttaacggtt cctatacatg atccattgcg
    20761 tgtcgggacg ctggcttcta ttttgaatga aattgctagg catcattcct tatccaaaga
    20821 agaagtaatt cttagattat ttgggaagat taatgaccac aattgaagtt gacgaaccga
    20881 tcatgctctc tgcgttgcag cactatagct attgtccacg ccagtgcgca ttgattcatg
    20941 tcgagcaggc gtttgacgag aatgtgcaca ccatgcgcgg taataccgtg catcaacggg
    21001 ttgatgaacc tggttccgag tcatttgaag gtgtgcgcag tgagaaagct ttaccgattt
    21061 ggtcagaacg tttaggtctg atcggcaaat gcgatatcgt cgaattttat tcgaatggac
    21121 gaatttatcc ggtggaatac aaacatggca agaaacgcgc aaaagcacat gatgatatcc
    21181 aattggcagc gcaagcgatc tgcctggaag aaatgacggg taaaacagtg acacatggtg
    21241 cgatttatca tcattcatcg cgccggcggc gtgaagtggt tattaacgaa ttgcttcgac
    21301 agcaggtgga agaaaccgtg caagcggttc gcgctttgct ggtatctggg aaattgccac
    21361 cgccagtcaa cgatgtacgt tgcaaggagt gttcgttgaa agaaatctgt caacctgagg
    21421 ttttggcaga taaggccaga gttcttgatg cccagataca tttgtttgaa gtggggtaat
    21481 agtaaattat gtatcaaatt ctcaacacac tttacatcat gacacccaat gcttatgtac
    21541 atttggataa tgatactgtg cgcattgatg ttgaaaggca aaaaaaacta caagtgccac
    21601 ttcatcattt aggtaccttg gtatgttttg gtaatatcat gatttcaccg gcgctgatgc
    21661 atcgttgtgc agatgacgga attggcttgg ttttgttgga tgctaatggt cggtttaaag
    21721 cacgtctcga aggtgctgta aatggtaata ttttgcttcg tcaaatgcag catcgcaaag
    21781 cgatagagtt ccctttttca cttgcgatag ccaaggccgt gatcgctggg aagttacgca
    21841 attgccgcca agtattgttg cgtggtgcgc gagaagcaac tgatgagcag gaccagggtg
    21901 tgcttagtaa tgcagctgca acgttggcaa actccattcg caatttacct accgcagcag
    21961 atcttgatgt gatccgtggc atcgagggtg acgccgctaa agtgtatttc tcggcactac
    22021 ccaatttagt tcggcacaat attcgtgagt attttacgat ggatggtaga acacgtagac
    22081 cacctcgtga tcgcttcaat gcgctactat catttcttta ttcactggtg atgaacgatt
    22141 gccgttccgc gattgaaagt gttggccttg atccgcaatt gggcttctta catagcgtac
    22201 gccctggcag ggcagcattg gcgcttgatc taatggagga atttcgctct attctcgccg
    22261 atcggcttgc attaacactg attaatcgta gtcaaattac tgagcatgac ttgaaaatac
    22321 gtgagggcgg tgcagtgcag ctggaaaacg atgcacgaaa agttgttgtg gctgcttatc
    22381 aggagaaaaa gcaagaagaa gtcacgcacc cattattgaa tactaaagta gcatttggct
    22441 tactgcctca gcttcaagca cgtttcatgg ctcgcgctat tcgaggcgat attgattcct
    22501 atattccatt tctgatcaaa taacgatgct gatcattgtc acttacgatg tttctacaga
    22561 aacaaaagaa gggagaagac ggctgcgccg ggttgctaaa atttgtgaag gaataggaca
    22621 acgtgtgcaa aaatcagtgt ttgaatgtaa agtcgatctc atgcaatatg aagaattaga
    22681 gcgcagatta ctggccgaga ttgaggaaaa agaagacaat cttcgtttgt atcgtttaac
    22741 agaacctgtt gctttacatg tcaaagagta tgggaaattt aaagcaatcg attttgatgg
    22801 aaccttggtt gtttaggatg cgcgaacaac gagtgattgt gaaatagcta taggttcgcg
    22861 gaaggtataa cattctgaat gttaatagaa tttaataaag taggctttta tttgtgttga
    22921 taaattttct acttgctgcc cagttcgcgc atttacaaag attttttctg ttgtatcatc
    22981 aagttgtagc tgtactgtag cgcccagcat ccaagctggg cgaggattga aacacggcat
    23041 ttacaaccgc aattgatggc gtgtctagta gcgcccagca tccaagctgg gcgaggattg
    23101 aaactagagt tatagctcaa caaagggctg caagggatgt agcgcccagc atccaagctg
    23161 ggcgaggatt gaaactttgt tctgtagtag ctccgccgta atacattgtt gtagcgccca
    23221 gcatccaagc tgggcgagga ttgaaacacc cctttcagct tgtcaattga tctctgacta
    23281 aaggtagcgc ccagcatcca agctgggcga ggattgaaac aggacaaaga aaataaaatt
    23341 atagtgaccg cgcttgtagc gcccagcatc caagctgggc gaggattgaa acaatttttt
    23401 cgactcgatc caaaagaacc tcatggcgta gcgcccagca tccaagctgg gcgaggattg
    23461 aaaccaaaca atagtcgatc cgtatggaag cccgaacgtg tagcgcccag catccaagct
    23521 gggcgaggat tgaaacaaat tggttgctgg cgaataagat gcagatatag gtagcgccca
    23581 gcatccaagc tgggcgagga ttgaaactta tcatgcgcca tatttttata gtggaacccc
    23641 tgggtagcgc ccagcatcca agctgggcga ggattgaaac tgaagctgcc aaaaatgtac
    23701 aatcagttat ccctagtagc gcccagcatc caagctgggc gaggattgaa acaaaaaatg
    23761 cagcttcgct aattgctgct cctactggta gcgcccagca tccaagctgg gcgaggattg
    23821 aaacttttct ggaaccaaaa actccagaag aaaaacaagt agcgcccagc atccaagctg
    23881 ggcgaggatt gaaacaccgg caagctagat aagtttgcta ctgagatgag gtagcgccca
    23941 gcatccaagc tgggcgagga ttgaaaccac atacaataat atgcaatata cccatcccac
    24001 gagtagcgcc cagcatccaa gctgggcgag gattgaaaca catctaatat tgaccataaa
    24061 acatctagct tgtagcgccc agcatccaag ctgggcgagg attgaaacaa ttgctaatga
    24121 tattagattg actaccttgg aggtagcgcc cagcatccaa gctgggcgag gatt
//
LOCUS       DAOQKQ010000014        24004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_880_length_24004_cov_6.033905, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000014 DAOQKQ010000000
VERSION     DAOQKQ010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 24004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24004
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_880_length_24004_cov_6.033905"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /gene="tyrS"
                     /locus_tag="PKZ49_01785"
     CDS             complement(<1..800)
                     /gene="tyrS"
                     /locus_tag="PKZ49_01785"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HND35321.1"
                     /translation="MCSSTNQANEQLKIIKRGCQELLVEEEVIQKLTRDHPLRIKAGF
                     DPTAPDLHLGHTVLLNKLRQFQDLGHHVLFLIGDFTGMIGDPSGKNTTRPPLTREEVT
                     ENAKSYTTQVFKILNPEKTEIVFNSTWMDKLTAADLIKLSAAYTVARMLERDDFSKRY
                     RENQAIALHEFLYPLIQGYDSVALKADIELGGTDQKFNLLVGRELQKHFGLTPQCILT
                     MPLLEGLDGVNKMSKSLKNYIGITDSPTEIFGKIMSISDSLMWRYIELL"
     gene            938..2284
                     /locus_tag="PKZ49_01790"
     CDS             938..2284
                     /locus_tag="PKZ49_01790"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HND35322.1"
                     /translation="MFYTPLPSKTRILAQKAPVVTQKGLRWLTIISSIPLFGIVTAFG
                     IAPTTSSLQDIPVRQIVHSLPLPEIAVTNEDENQYSVSTFWHQENIRRGDTIAAILSR
                     LEVNKQDKADFIRAVRDNKVMQHLRSGEIIHAETSTEGELLTLRYFNIQGKQLNIGKI
                     GSFYEIDNETAETNARIRVGSGIIQNTLFAATDAAGLPNGIAAKIVEIFASDIDFYRD
                     LQKGDRFTVVYESLQDYGSTIQSGRVLAVEFINKGKPYRAVYFQEPGGEGDYYNPNGE
                     NLRKPFLRTPLEFSRISSGFTNARFHPILKQWRAHKGVDYAAPTGTPVMATADGVVEF
                     KGWQNGYGNMIILKHRGEYSTAYGHLSSFGRGLQKERRIKQGEIIGYVGSTGMATGPH
                     LHYELRVNGIQRDPSQVIMPAALSISKRNKAIFQKETKELIARLDLLRNANFASAK"
     gene            2334..3428
                     /locus_tag="PKZ49_01795"
     CDS             2334..3428
                     /locus_tag="PKZ49_01795"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HND35323.1"
                     /translation="MGQELYVGVMSGTSLDGIDVVLVDFRLSPPKLVKTYFLPYCASL
                     KKDLLALHLQGENELHRSALLANVLSSLYAQAINNLLIVAEISSESIIAIGCHGQTIR
                     HCPDSGYSIQLFNPALLAELTGITVVADFRSRDIAAGGQGAPLVPAFHQACFGHQTSH
                     RIIVNIGGIANITSLSPEDIVIGFDCGPGNMLMDAWCFENQGQSYDDNGNWASKGESH
                     SLLLEALLSYPFFSLPPPKSTGRDCFNLDWLRMHLNGTELPVDVQATLLELTVQSIVR
                     SIQSYFPAATEIYLCGGGAHNARLVNRISEAFLEKKVALTDQLGIAVDWVEAFAFAWL
                     ARQTIHKKPSNLPAVTGAKGERILGAIYYA"
     gene            3745..4314
                     /locus_tag="PKZ49_01800"
     CDS             3745..4314
                     /locus_tag="PKZ49_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35324.1"
                     /translation="MLNLKGSSILVGCALLLSFSLQAENKSDNAGKHLALNLVGTGTM
                     YENMVPDIDGDGQDDLAMCFDVQVFDAHTNRALGKGTDCLAMVTPTGTGVALVGTTYF
                     HLPGGILITRGKTSVQPVFHPTVTPRGQVITHITGASSDGNAIVGGTGRFAHAKGTAR
                     LSGMVSMANFSGDVGDPISFDCLFVVALD"
     gene            complement(4443..5834)
                     /locus_tag="PKZ49_01805"
     CDS             complement(4443..5834)
                     /locus_tag="PKZ49_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HND35325.1"
                     /translation="MLKIKKIQILMLSIAISSVLLLLPYQVEAASTAQTKKNTYAAKG
                     RIASANRNNNVGDLWTRVRSGFSMSELQGHEVRQNENGYTRHQKFIDHMVEQSRRYLF
                     HIVEEVERRKMPTEIALLPIIESAFNPVAYSHRHASGLWQFVPATGRAYGLEQNWWHD
                     DRRDVIAATNAALDYLQKLYRMFGDWKLTVAAYNWGEGALKRSIDENHGGDLKVSFHD
                     LQLPTETKNHVAKLIAIRNIIANPSQYGVKLKPLPNRPYFKQVKITQHMDVKLAAELA
                     DLPEHEFNALNPAYHRPVIRIKDKPRTLLLPVGSVKTFEVNLENYDKSLVSWRVYRVK
                     ENETLDDLSIKYRVDIAELAQVNGLSEYDTIEEGQTLLVPRKRNRNSNDDRLFVAQPT
                     FSELHMQPVKVSPIIHIVQKGDTLYDIAKRYSVDVRELKSWNGNKERLAIGQKLIVRL
                     ADSNRNQSVTKPS"
     gene            complement(6083..7270)
                     /locus_tag="PKZ49_01810"
     CDS             complement(6083..7270)
                     /locus_tag="PKZ49_01810"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HND35326.1"
                     /translation="MLTFDDEPLQSEAIQGKSILSNSRPDWAESTGITPVAPPPSTIS
                     LPQNEQLAEDIALRRVSVEDKRIINCKTDVNQLVPFKYKWAWEKYLSACANHWMPQEI
                     SMSRDIALWKDPNGLTEDERRLVKRNLGFFVTADSLAANNIVLGTYRHITNPECRQYL
                     LRQAFEEAIHTHAYQYIVESLGLDEGEIFNAYHEIQSIRDKDEFLIPFIDTLTDPKFK
                     TGTLETDQQLLKSLIVFACIMEGLFFYVGFTQILALGRQNKMMGAAEQYQYILRDESM
                     HCNFGIDVINQIKLENPHLWTKEFREEITTLMHKAVELEYRYAEDTMPRGVLGLNAAM
                     FKEYLRFIANRRCQQIGLDALFPEASNPFPWMSEMIDLKKEKNFFETRVIEYQTGGVL
                     SWD"
     gene            complement(7313..10183)
                     /locus_tag="PKZ49_01815"
     CDS             complement(7313..10183)
                     /locus_tag="PKZ49_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HND35327.1"
                     /translation="MQLTANSTSTVLNPIPHGEFSEGSSNQGVQLSLYKIIRRNGAVV
                     AFEPSKISVAMTKAFIAVEGGQGAASARVREVVARLTEEVVNALTRRQSGGGTFHIED
                     IQDQVELALMRSGEHDVARSYVLYREERARERAKQQEKQTTAKTPLIHIVENGQRIPL
                     DTKQLTELVDAACADLGNSVDSSLILKSTLKDLYDGVPANEVRKSLILSARALIEKDP
                     DYSFVTARLLLHSIRLEALGENISQKEMESRYADYFPNFIKQGIDAGLLDERLAQFDL
                     TQLAAELVASRDMQFGYLGLQTLYDRYFLHINDKRIELPQAFFMRVAMGLALNEIDRE
                     ARAIEFYHVLSKFDFMSSTPTLFNSGTCRPQLSSCYLTTVPDSLDSIYEAIKENALLS
                     KFAGGLGNDWTPVRAMGSRIKGTNGKSQGVVPFLKVVSDTAVAVNQGGKRKGAVCAYL
                     ECWHLDIEEFLELRKNTGDDRRRTHDMNTAVWIPDLFMKRVMENADWTLFSPSDTPDL
                     HEKYGKAFETAYLSYEEKASRGELSLYKQVSAQQLWRKMLTMLFETGHPWITFKDPCN
                     IRSPQSHVGVVHSSNLCTEITLNTSDKEIAVCNLGSVNLLAHIKDSKLDTEKLKRTIS
                     TAMRMLDNVIDINFYAVTKARNANLKHRPVGLGIMGFHDCLYQLGISYASQEAIEFAD
                     RSMEAVAFHAYWASTELAEERGCYATYRNSLWDRGILPQDTLDLLRDERGGFVDVDTT
                     TTLPWDKLRARIKKHGMRNSNCLAIAPTATISNIIGVSASIEPTYQNLYVKSNLSGEF
                     TIANRWLVNDLKKHELWDEVMIADLKYYDGAIGKIDRIPPLIRSQYATAFEVDPYWLI
                     ESAARRQKWIDQAQSLNIYIAGVSGKKLDEIYKLAWLRGLKTTYYLRSLGATTAEKST
                     VYTGALNAVPTSAGATMANAQQTAATEIKYCAIDDPGCESCQ"
     gene            complement(10520..10741)
                     /locus_tag="PKZ49_01820"
     CDS             complement(10520..10741)
                     /locus_tag="PKZ49_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35328.1"
                     /translation="MKYIIIAAALAGLSLAGCGGKPSQALPTTEKEAYEKIISGQSLD
                     CQFGIDANGNCLKEGEDPRPYGGKGKPGH"
     gene            complement(10894..11676)
                     /locus_tag="PKZ49_01825"
     CDS             complement(10894..11676)
                     /locus_tag="PKZ49_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HND35329.1"
                     /translation="MTHALKLWPLLTGTIRYEKTISTHNRGHGQWIDAPILAYLIETT
                     QGRILYDVGCDYHKLSDPALRQQFFASMYPVIETPQMQAEQRIPHYLERLGLTTADID
                     LILIGHLHFDHVGGLCDLPGCEVHLHQAELAAARTKQDSGVFPDELLQSDRWRLQHEE
                     YELAPGVQVLTTPGHTAGHLSLMIELPQGQPIILCGDAADLNENLTDEIAPGCCWQDN
                     FELALASIRKLKSIAISEQAQLWPNHDMVFFKQLPVFPTGHE"
     gene            complement(11723..12625)
                     /locus_tag="PKZ49_01830"
     CDS             complement(11723..12625)
                     /locus_tag="PKZ49_01830"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HND35330.1"
                     /translation="MSQQSNSSSISTDERIISGSNGWVMLVSLLALFAFSLFFTMTPG
                     PPIKLFGGILLAMLALFGCKGLFTLEPNQAAVMVFFGNYVGTVNESGFFWVNPFYNKT
                     RVSLRINNWNTPVLKVNDERGSPIEIAAVIAWRVQDTARAIFDVESTLNYLQIQSESA
                     VRQVASSHAYDDTEMIDGKRKKSLRTDLDAIAGLLRESIQEHVNVAGIVIEEAKIAHL
                     AYAPEIANAMLRRQQAEAVVMARQKLVDGAVGMVEVALKNLEEKQIVSLTSDQRAVLV
                     TNMMTVLLSESGAQPVIQMTQTTP"
     gene            12792..13364
                     /locus_tag="PKZ49_01835"
     CDS             12792..13364
                     /locus_tag="PKZ49_01835"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HND35331.1"
                     /translation="MPFSFSNQLSAIIGYAEQQKPLSILDIGVGMGQYGFLLRTNLEN
                     INLFEINGANARLRDKNQWRIKIDGIEGYAGYLTPVHNYAYNRILIGDALELLPTLAS
                     NSYDLVLAIDILEHFYKEQGVHFLKECRRIAKGSSLISTPKEFMEQDVPANPFENHRS
                     VWNDEDLKRCGFDTFLPNPFSWIALASHHE"
     gene            13477..13938
                     /locus_tag="PKZ49_01840"
     CDS             13477..13938
                     /locus_tag="PKZ49_01840"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HND35332.1"
                     /translation="MLRIKLLGCFIAIVSMLFSFSAFAYKEHSAEPHSAASGVSALQK
                     IDTTVGNGEEAIVGKTVKVHYSGWLYDESAPDKKGKKFDSSYDRKEPFAFMLGAGRVI
                     KGWDQGVIGMKVGGQRTLIIPSSMAYGAQGAGNVIPPHATLIFDVELVGFE"
     gene            14018..17392
                     /locus_tag="PKZ49_01845"
     CDS             14018..17392
                     /locus_tag="PKZ49_01845"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HND35333.1"
                     /translation="METDISSPTKKRFLTRKHLAISLGVIFSIVVLFGVLGYFWLPGF
                     AKAKLEQVLSETLARPVTVQSIDIKPFTLEVAVRGFRIGEKSTVSDQTTFFSFDQLYV
                     DLSAESLIHLAPVVTTISLVAPKIYVTREDKNQFNFSDLIEKFGQTPEEKPQEPKKPT
                     LFSINNISIQNGEITFADRIKNSQQHITAINLSIPFIANFKNVLTNWVEPNLSAKINE
                     APVTLSGKVLPFSDKQEATLSLKLDDIDLTNIDEYSPIPLGIRLLSGKFDSDLLVSFT
                     HVIDEPPNIDLSGQIALKGIQIENRTVEMPYILGVKQFNLKLNEVSFNETKPVKVGTT
                     FESIAITPIGEKQALLSLPKIELDSITVDRARHKIDLGTILLDGINGLIKRQPDGQLE
                     LNRMLAVTASSNSMQSEEDKVKKLSKPIQRNVSAIIPIPDKKPDNRVTSLAQTQAREQ
                     ELVAPSVITIPLPEHKPQIVARPEDTKQPQANMGKMAEETSSQSAPAAEETDQPWAIY
                     IDQIKLSDGAITFEDYTVSKVVPMHINPLNLTVRNIDLSGAQPVELSLQAQVNQQGSV
                     ETKGTLAWAPLAADLVLDLKQVDLVPLQGWFGNQLNILLSKGNFSFQGDVKADGEPLK
                     IALQGKAQLANFNVLDKVKLAEQLRWKSIDVTGIQFINEPLQIGMTTIDLTDLFARVI
                     LLADGKINLRDLTEANKAEASPVKPESTPVASAPNQAEAQGAANNKAPLPIKIDKVTL
                     KNTHINFTDQFIKPNYRVNLTELKGQVSPLHPGKAGKVDIRGTIDKSAPLQISGTIDP
                     FSEQLSFDIATTVKGIDLPTFSPYSGKYIGHLIEKGKLSVDVNYQIQQGQLSAENKIF
                     LDQLKIGEKVDSPDAVSLPLDLAISLLKNRKGEINLRFPVSGSINDPQFSISGVVWEA
                     FINLISKALTAPFALLGSFLGDGEELAEITFPSGYATLEPEAINHLQSLADALIDRPE
                     LKLEITGYADPLNDYETLKQVLLDRKVKTQKLSETVNKGQSVDLLDEIKLDDAEYARY
                     LEIVYKASDFEKPKNMIGLSKSLPVDEMKQLILTNTKVSDEALNELAEKRASVVFHWL
                     IEQGGVPSERVFVLGTHLESASAAEKPYSKAAFALK"
     gene            17426..18499
                     /gene="mutY"
                     /locus_tag="PKZ49_01850"
     CDS             17426..18499
                     /gene="mutY"
                     /locus_tag="PKZ49_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HND35334.1"
                     /translation="MPNFANLLIRWQQQHGRHDLPWQGTQNPYLIWVAEIMLQQTQVK
                     TVIPFYQRFIETFPTIAALAKADLDDVLAMWSGLGYYARGRNLHKAAGVIMNDYQGKF
                     PTCSSEIQKLPGIGRTTAAAIAAFCYGEPCTILDGNVKRVLARYFGIEGYPGLNATQQ
                     RLWEKAEAVLPRGNTQKNMPAYTQALMDLGAIICTRTSPRCTACPLQSHCTAFLENRI
                     SCLPTPKPSKALPSKETVFLLLLHHDKLLLEKRVNQGIWKELWCLPEAEKNDHYQNYC
                     AQMLGIDAVCVDLLLPPIDHTFTHFKLRMYTQRLQVKSIIQQPQCNPIRWVTTDEAFQ
                     LAIPTAIKKILSKYRAASTPTSL"
     gene            18509..19138
                     /locus_tag="PKZ49_01855"
     CDS             18509..19138
                     /locus_tag="PKZ49_01855"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HND35335.1"
                     /translation="MNDWQNWRKQQRAELIAQRMQISSSDRILWNQAITHFLIQGFPI
                     LQQHAVGFYSAYKGEYDPMPVMEHLNACGTTLALPEVIESGQPLIYRKWWPQVSIKKD
                     RYGLSVPCDTEAVLISAVMIPMVGFDDQGYRLGYGSGYFDRTLAAMTTHPLTIGIAFE
                     QQRIATTYPQSHDIPMDFIVTERSIYALNQRLHPLSIENCARVSTQSQS"
     gene            19297..19539
                     /locus_tag="PKZ49_01860"
     CDS             19297..19539
                     /locus_tag="PKZ49_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HND35336.1"
                     /translation="MTILKATEARTRLYNLIDEAAETHQPIVITGKRNNAVLISENDW
                     NAINETLYLVSIPGMRSSIREGMESNLSECSKEIDW"
     gene            19536..19799
                     /locus_tag="PKZ49_01865"
     CDS             19536..19799
                     /locus_tag="PKZ49_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HND35337.1"
                     /translation="MNWEIYYTKQAQKDALKISQAGLKPKVVELLKLLVENPLQNPPS
                     YEKLVGDLTGAYSRRITIQHRLIYQIYPEDKKIKVLRMWTHYE"
     gene            19909..20118
                     /locus_tag="PKZ49_01870"
     CDS             19909..20118
                     /locus_tag="PKZ49_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35338.1"
                     /translation="MKKSGKKNPTWNDLKRQLANFDHTGLLCLIQDLYVADKQNQAFL
                     HARFGLVTMRSHRTKPLLIAGFVPM"
     gene            20118..20453
                     /locus_tag="PKZ49_01875"
     CDS             20118..20453
                     /locus_tag="PKZ49_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35339.1"
                     /translation="MRNQDISVAKAKKAVSDYKKAIGRPEGIAELTVFYCESCMDFLG
                     YCGMDDEGYFNALILMFEQALKAIQNLDVKQQAGFVERLNVLQHAGHNWGMGDEIEDL
                     MVKYGFTKT"
     gene            complement(20822..23443)
                     /locus_tag="PKZ49_01880"
     CDS             complement(20822..23443)
                     /locus_tag="PKZ49_01880"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HND35340.1"
                     /translation="MKLRLRTSSGKYLSCDGASDTTPLNAGKSNKGETEEFSLVINNQ
                     VISSDHRGTLRISSGDRGTLRTTRNLTTFHMSQNYRQDAYVTTIDSRGRHQNRLFVQT
                     HFFGEKGDDFIITRLNRDGEVLLDGDEIALKFVETDKPGFLSSDSSGLLRIINKDNPG
                     SQEIFVVETMSELKLFWSDQRADNFSTATPLGELQAIQAGYAFVRTQGLVYMNSMPGC
                     NPLFLFWNNQNGDNLITATKEGEKEANDNGYVKVRIEGWIKSKPEPGTIPLRMYWKGA
                     ERKDSFTTTSIKEEQDAANAGYEFVRDEGYIIPPLSVIIPSVSILTPGTIQPNFDSIE
                     GVGNQNIDNSDINREGLINININSPRRRRGANKKALILSGGGAKGCFEVGAVKQLWSK
                     GWRPDIICGVSVGALNAAKLAERKDTSPAELEEIWRELRPEKRGGRAVYSKDYFVNLL
                     STWIPNMLSDGIDDLIGAKDIFEWLKYAASHLHAIHSMHPLRHLIHRNLNIGAIRTGG
                     TQLRIGITDLRTGQYFSVTEPFEAGALGLNMCGRIELEPDHRMGETWLTRPIFGADAY
                     AMSIEDAVYSSSVLPVFMDPKIVNLRTARPISYGNEKIVMLKVPGTAFNATYPLPAIE
                     RIMQFTRGEQPFGSQVFKDLKNSFAPPYNLDQILKESDDVFKGDSRQSQYPLFDGGLR
                     DTMAIRTAIRLGAREIVVITGDRLQAAHWNFKNPGLINSEEGKVDFSALPVAQYLFGL
                     LNVWFNESARNDVMVSIAQNEFLGWLYRCFSLMDNDKRRQIVAEFNEYWAKKGPILER
                     TLGGSSWIGGDLAKNYGTPFQDEGCSINYIAPQKDLVDALGFDRWDEIEEGIQMGYEA
                     ANSPVELSFPVPDHSIR"
     gene            23703..>24004
                     /locus_tag="PKZ49_01885"
     CDS             23703..>24004
                     /locus_tag="PKZ49_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35341.1"
                     /translation="MNGKSPDWISLIETSYSLEGNDSQWLRGLIDHVSKILNRGSEPV
                     GWTFHCTPTKFKLGSFSEGTSKALTYAARMSHALSTEKSLDLTYRTGVVIATASELV"
BASE COUNT         7065 a         5089 c         4924 g         6926 t
ORIGIN      
        1 agtaactcaa tgtaacgcca cattaagcta tcagaaatgg acataatctt tccgaaaatt
       61 tcggttggac tatctgtaat cccgatgtaa ttttttaaag atttagacat cttattgacc
      121 ccatctaaac cttccaataa gggcatggtc aagatacatt gtggggttaa gccaaaatgt
      181 ttttgtaatt ctcgacccac taataaatta aatttctggt cagttcctcc taactcaata
      241 tcagctttaa gtgcaactga gtcataacct tgtatcagtg gatacaagaa ctcatgaagt
      301 gcaatggctt gattttcccg ataacgttta ctgaagtcgt ctctttctag catacgcgct
      361 acagtgtagg cagcagatag tttgattaaa tcagctgcag ttaatttatc catccaagtg
      421 gaattgaaga caatttcagt tttttctgga ttgagaattt taaaaacttg ggtagtgtaa
      481 gattttgcgt tctcagtcac ctcttccctc gttaaagggg ggcgggtcgt attcttacca
      541 ctgggatcac caatcattcc agtgaaatcc ccgattagaa ataaaacatg gtggcccaaa
      601 tcttggaatt gacgaagctt gttgagcaat accgtatgcc ccagatgcaa atcaggcgct
      661 gtaggatcga aaccagcttt gatcctgagc ggatgatcac gggtgagttt ttgaataact
      721 tcttcttcga ccaataattc ttggcaaccc cgtttgataa tttttaattg ctcattggct
      781 tgattagttg aggaacacac gatttttatc taagtgatta agttttaaga gaaaagggtt
      841 gagaaaccta ttcagcgcta acgagttatg ctagggcgca ggccattttt tatgctagac
      901 ttagcgcaac tcacaatata taggagtgat acgtagtatg ttttatacgc cattacctag
      961 caaaacaagg attctagcgc aaaaagcacc agtcgtaaca caaaaaggtt tacgttggtt
     1021 gaccattatt tcgagtatcc ccttatttgg tatcgtaacc gcatttggaa ttgctccgac
     1081 gacatcttct ttgcaagata ttccagttcg tcaaattgtc cactcgcttc cgttgcctga
     1141 gatcgctgtt accaatgaag acgaaaatca atatagcgta agcactttct ggcaccaaga
     1201 aaatatccgt cggggggata ccatagcagc tatcctttcc aggcttgaag taaacaagca
     1261 agataaagca gattttatac gtgctgttag ggacaataaa gtcatgcagc atttaagatc
     1321 tggagagatc attcatgctg aaacttcgac agagggagag ctattaacct tacgatattt
     1381 caatatacag ggtaagcaac taaatattgg aaaaatcggt agtttctatg aaatcgataa
     1441 tgaaacagca gaaactaatg cacgcattag ggtaggatcg ggcatcattc aaaatacctt
     1501 atttgcagca acggatgctg cgggattacc gaatggcatt gcagcgaaaa tagtcgagat
     1561 ttttgcttcc gatatcgatt tttaccgaga tctacaaaaa ggggatcggt ttaccgtcgt
     1621 ttatgaatct cttcaggatt atggatcaac gattcaatca ggccgcgttc tggcagtaga
     1681 atttattaac aaagggaaac cttatcgcgc agtttatttt caagaacccg gtggagaagg
     1741 agactactat aatcccaatg gtgagaatct cagaaagcca tttttacgaa ctccacttga
     1801 gttttcacgt attagctcag gatttaccaa tgcccgtttt catccaatct taaagcagtg
     1861 gcgggcccat aaaggggttg attatgctgc accaacaggc actccagtga tggctacagc
     1921 agatggtgtt gtcgagttta agggctggca gaatgggtat ggcaatatga tcattctaaa
     1981 acatcgtggt gaatacagta ctgcttatgg tcatttgtct tcatttggta gagggttgca
     2041 aaaggaaaga agaattaagc aaggggaaat catcggatat gttggttcga ccgggatggc
     2101 taccggccct catcttcact atgaacttcg ggtgaacggg attcagcgcg atccatctca
     2161 agttattatg cctgctgcgc tatcaatcag taaaagaaat aaagctatat ttcagaaaga
     2221 gactaaagaa cttattgctc gccttgattt acttcgcaat gcaaactttg cgtcagcaaa
     2281 gtagcatagg aatcgtcggt acccaattag attagcattt gggaatccag ccattgggcc
     2341 aagagctgta tgtaggagta atgtctggca caagccttga tggtattgat gttgtattag
     2401 tagattttag gctcagtcca cccaagcttg taaagaccta tttcttaccc tattgcgcta
     2461 gtttaaagaa ggatttattg gcgttgcact tgcaaggcga gaatgaattg caccgttctg
     2521 cattactcgc taatgtatta tcaagtttat acgcacaagc aataaataat ttactgatcg
     2581 tagcagagat cagctcagaa tcgattatag caataggttg ccatggtcag accataagac
     2641 actgtccaga ttctggttat agcatccaat tgtttaatcc agcgttattg gcagaattaa
     2701 ctggtattac tgtcgttgct gattttagga gtcgtgatat tgctgcagga ggccaaggcg
     2761 caccactggt tcccgcattc catcaggctt gttttggtca tcaaacatct catcggatta
     2821 tcgtgaatat tggcggtatt gcaaatatta caagtctttc acctgaagat attgtaattg
     2881 gttttgattg tggccctggc aatatgctaa tggatgcttg gtgctttgag aaccaaggac
     2941 aaagctatga tgataatggt aactgggcaa gtaagggaga atcgcattcg ctgttgctag
     3001 aagcattatt atcgtatcct ttcttttctt tacctccccc caaaagcact ggtagggatt
     3061 gctttaatct tgattggctg agaatgcatt taaacggaac tgagcttcct gtagatgtgc
     3121 aagcaacctt actcgaattg acggtacaaa gcatagtgcg gagcatacaa tcttactttc
     3181 ctgctgcaac agaaatttat ctatgcggcg ggggagcgca taatgcaagg cttgtgaatc
     3241 gaataagtga agcatttctg gaaaagaaag ttgcactgac tgatcaatta ggaatcgctg
     3301 ttgattgggt agaagctttt gcttttgcct ggcttgcacg acaaacaatc cacaaaaaac
     3361 ctagcaatct acctgctgta actggtgcca aaggagaacg tattctaggt gcaatttact
     3421 atgcctagtt agtcctaaaa atattagagc cacaagatct tatagcggta tttctttctt
     3481 taaaacgcta aatagcagca cttgatatga aattgattca tttaaggtgt aagaaattat
     3541 gcttcagtta agtttgtggc atgaccagaa tcaaaagatt acaaatcttg ccgtgataaa
     3601 aatcacatcg tatcaatacc taaatcaata tcctgcaaaa taatttgaaa tttagcttct
     3661 tgaggtgatt tttagaaata aaacacaaca agaagctaga tatggggtgt tgttgattaa
     3721 tttttgatag gagagattat tgatatgttg aatttaaaag gaagctcgat tttagttggc
     3781 tgtgcattat tattatcatt ttcacttcag gcagaaaata agtcagataa tgcaggcaag
     3841 catcttgccc taaatctcgt aggaacagga acaatgtacg agaatatggt tcctgatatt
     3901 gatggcgatg ggcaagatga tctggctatg tgtttcgatg ttcaggtttt tgatgcgcat
     3961 acaaaccgag cgttaggcaa aggtaccgat tgtttggcga tggtgacacc aaccggaacg
     4021 ggggttgcat tagttggcac tacttatttc catttaccgg gtggtatttt aattacacgt
     4081 ggaaaaacct cagttcaacc tgttttccat ccaactgtaa caccccgcgg tcaggtgatt
     4141 actcatatta cgggtgcatc cagtgacgga aacgcgattg ttggtggtac aggtcgcttt
     4201 gctcacgcga aaggaacagc gaggctatct ggcatggtta gcatggcaaa tttttcaggt
     4261 gatgtgggag accccatttc gtttgattgc ttgtttgtag tagcattaga ctaattaaca
     4321 tcagagaacg ctacgtggga aggctacttg cagcctgata tttaaattgt gaccggctcg
     4381 gtaatctttc cgcttttgtt cgtttgtact ttagattcaa aaaaatatca gagtttgagt
     4441 agtcaagagg gtttagtgac cgattgattt ctattcgagt cggctagcct cacgattaat
     4501 ttctgcccaa ttgcaagccg ttctttattt ccattccatg atttaagttc ccttacgtct
     4561 acgctataac gctttgcgat gtcgtacagt gtatcccctt tttgtacaat atgaataata
     4621 ggggatacct tcactggttg catgtgtaac tcgctgaagg tgggttgtgc tacaaatagg
     4681 cgatcatcat tgctgtttct atttcttttg cgtggcacaa gcaacgtctg tccttcttca
     4741 atcgtatcgt attcagagag accattgact tgtgctagct ctgcaatatc gactcgatac
     4801 ttgatggata aatcatctaa ggtttcattt tctttaacac gatagacgcg ccaggaaaca
     4861 agcgatttat catagttttc taaattcact tcgaaggttt taacactacc aactggaagt
     4921 aatagtgtgc gtggcttatc tttgatgcga ataaccggtc gatgatatgc tggatttagt
     4981 gcattaaatt catgctctgg gaggtctgcc agttccgcag cgagtttaac atccatatgc
     5041 tgcgtaattt ttacttgttt aaaataaggt cgattgggga gcggtttaag tttgacgcca
     5101 tattggctag ggttggctat gatatttcta attgcaatta gctttgctac atggttcttt
     5161 gtttccgtgg gtaattggag atcgtggaag ctaactttta aatccccgcc atgattttca
     5221 tcgatactgc gtttgagcgc accttccccc caattatagg cagcaaccgt cagtttccag
     5281 tccccaaaca ttcggtatag tttttggaga taatcgagtg cagcattagt tgcagcgatt
     5341 acatcacgac gatcatcgtg ccaccagttc tgctctaacc cataagcacg accggtcgcg
     5401 ggaacaaatt gccagagacc tgaggcatgg cgatgagaat atgcaacggg attaaatgca
     5461 ctttcaatga tcggtaatag tgcgatttca gtaggcattt ttctccgttc gacttcttct
     5521 acgatatgga aaagataccg cctactctgc tcaaccatat ggtcgataaa tttttggtga
     5581 cgagtatagc cattttcatt ctggcgcact tcatgtcctt gtagctcaga catagagaat
     5641 ccgcttcgga ctctggtcca tagatcgcct acattgttat ttctatttgc tgaagctatt
     5701 cttccttttg ctgcataagt atttttctta gtttgcgccg tgctagctgc ttcaacttga
     5761 tatggtagta atagcaatac tgagctaatt gcgatactaa gcatcaggat ttgaattttt
     5821 tttattttta gcataagtat gttcatcctt aattttcaaa aatagtcaaa cttctgaaaa
     5881 ttcagattca gttacatctg ctgatagaaa aaccacttga taacgcagtg cgttaaatag
     5941 aaatatacgg ccttacaaaa acgcgaagtg gtattcagca gaaaggcact acacaacgat
     6001 gcagtgccaa ttcatgatta ttttaaggtc cctaaaagta ggtagcgtta attttggcta
     6061 ggccagattg agatttatca atctaatccc aactcaatac accaccggtt tgatattcga
     6121 tgacgcgtgt ttcaaagaaa tttttctcct ttttaagatc aatcatctcc gacatccaag
     6181 gaaatggatt gcttgcctct gggaagagtg catctaagcc aatttgctgg caacgccgat
     6241 tggcaataaa acgtagatat tctttaaaca tcgctgcgtt caatcccaat acgcctctag
     6301 gcattgtatc ttcagcataa cgatattcca attctacagc tttgtgcatc aaagtggtaa
     6361 tttcttcacg aaattctttg gtccaaagat gcggattttc taatttaatt tgattgatga
     6421 catcaatacc gaaattacaa tgcatcgact catcgcgtaa aatatattga tactgctcgg
     6481 cagcacccat catcttattt tgcctaccta gagctaaaat ttgtgtgaag cctacataaa
     6541 aaaacaaacc ttccataata caagcgaata caattagact cttcaacagc tgttgatcag
     6601 tctctagcgt acctgtttta aattttggat cggttagagt atcaataaaa ggaattagaa
     6661 attcatcttt atcgcgaatt gactgaattt catgataagc gttaaaaatt tcaccttcat
     6721 ctaaaccgag cgattcaacg atatattggt aggcatgggt atgaattgcc tcctcaaaag
     6781 cctggcgcaa taaatattgg cggcactctg gattagtgat atgacgataa gtacccagta
     6841 cgatattgtt ggctgcaaga gaatctgcag taacaaagaa gcccagatta cgcttcacta
     6901 atcgccgttc gtcttcagtt agcccattgg gatccttcca gagtgcaata tcacggctca
     6961 tgctaatttc ttgcggcatc cagtgatttg cgcaggcaga taaatacttt tcccaagccc
     7021 atttatattt gaaaggaacg agttggttaa catccgtctt gcagttaata atgcgtttat
     7081 cttccacgga gacccgtctc aacgcgatat cttctgccaa ctgttcattc tgtggaagag
     7141 agatggtgct aggaggcggt gcaacagggg taattccagt cgattcagcc caatccggtc
     7201 tgctattact taagatcgat ttgccttgaa tcgcttctga ttgcagtggc tcatcatcaa
     7261 atgtcagcat tgttattact ccttctatgt tttatttata agcacatacg ctttattgac
     7321 aagactcgca acctggatcg tcaatcgcac aatatttaat ctcagtggct gctgtttgtt
     7381 gtgcattagc catggttgct cccgcactag tcggtactgc attgagcgcg cctgtataga
     7441 cagttgattt ctcagcggtg gttgctccta aggaacgtaa gtaataagta gttttgagtc
     7501 ctctcagcca agccaattta taaatttcat ctaatttttt accggatacc cccgcaatgt
     7561 aaatatttag agattgcgct tgatcaatcc atttctgccg tcgagctgca gattcaatca
     7621 accagtacgg atcaacctcg aaggcagtcg catattgact acgaattagc ggtggaattc
     7681 gatcaatctt accaattgca ccatcatagt atttcagatc tgcaatcatg acttcatccc
     7741 aaagttcatg tttcttgaga tcgttgacta gccatcgatt ggcgatggtg aattctcctg
     7801 ataaattcga tttgacgtat agattttggt aagtcggttc aatgcttgct gatacgccga
     7861 taatattgga aattgtggca gttggtgcaa ttgctaagca gtttgaattg cgcatgccat
     7921 gtttttttat gcgtgcccgc agtttgtccc atggtaatgt tgtagtcgta tccacatcaa
     7981 caaagccacc gcgctcatca cgaagcaaat caagcgtatc ctgaggcaag ataccgcgat
     8041 cccaaaggct attacgataa gttgcatagc aaccgcgctc ttccgccaac tcagtcgacg
     8101 cccaataagc atggaaagcg actgcttcca tcgaacgatc agcgaattct atcgcttctt
     8161 gcgaagcgta agaaatacca agttgataca aacagtcatg aaaacccatg atgccgagtc
     8221 caaccgggcg atgtttgagg ttagcattac gcgctttagt gacagcataa aaattgatat
     8281 caatcacatt atcaagcatc cgcatcgcag tgctaatcgt gcgcttgagt ttttcggtat
     8341 caagcttact atctttgata tgcgcgagta ggttgacgga tcctagatta catactgcaa
     8401 tttctttatc actcgtattc agtgtaatct cggtgcagag attagagcta tggacaacgc
     8461 ccacatggct ttgcggagaa cggatattac agggatcttt aaacgtaatc caaggatgcc
     8521 cagtttcaaa cagcatcgtt agcattttgc gccacagttg ctgcgcagaa acttgcttat
     8581 ataaggataa ttcaccgcgc gacgccttct cttcataact caaataggct gtttcaaagg
     8641 ctttaccata tttttcatgg agatcagggg tatctgaagg ggaaaacaat gtccaatccg
     8701 cattctccat tacccgtttc atgaataaat ctggtatcca aacggccgta ttcatgtcat
     8761 gtgtacggcg gcgatcatca ccggtatttt tgcgcaattc aagaaactct tcgatatcga
     8821 gatgccagca ttctagatag gcacagactg cccctttacg ttttcctcct tgattgacgg
     8881 caaccgcagt atctgaaact actttaagaa aaggaactac accttgtgac ttgccattgg
     8941 ttcctttaat ccgcgagccc attgcgcgaa ccggtgtcca gtcgtttcct aatccacctg
     9001 caaattttga aagtaacgca ttttccttta tcgcttcgta aatactatcc aggctatccg
     9061 gcactgttgt cagataacag gaagaaagct gaggacgaca agttcccgaa ttgaatagtg
     9121 taggtgtcga gctcatgaaa tcgaattttg acaaaacatg ataaaactcg attgcacggg
     9181 cttcccgatc aatttcattg agtgcgagac ccatcgctac tcgcatgaaa aaggcttgtg
     9241 gcagttcaat acgcttatcg tttatatgca gaaaataacg atcatacaaa gtctgcaaac
     9301 ccaggtagcc gaactgcata tctcgacttg caaccaactc agctgccaat tgagtcaaat
     9361 caaattgcgc taagcgctca tcgagcaaac cggcatcaat gccttgttta ataaaattag
     9421 ggaaataatc agcgtagcgt gattccattt ctttctggga aatattctca cctagcgctt
     9481 cgagtcgaat agaatgcagc agcaatcgag ctgtcacaaa gctataatca ggatcttttt
     9541 caatcagcgc acgtgccgat aatatcagcg attttctaac ttcgttagca ggtacgccat
     9601 catacagatc tttaagcgtg gatttaagaa tcagtgagct atcaacgcta ttgcccaaat
     9661 cggcacaagc tgcatcaact aattccgtta attgtttggt atcgagcgga atgcgctgcc
     9721 cattttctac aatgtgaata agcggcgttt tggctgtagt ttgtttttct tgctgttttg
     9781 cacgttcgcg ggctcgctct tcacgatata agacataaga acgcgcaaca tcatgctcac
     9841 ctgaccgcat caaagctaat tctacttgat cttgaatgtc ttcaatatga aaggtgccac
     9901 cgccagattg acgtcttgtc aatgcattga caacttcttc agttaatcga gctactactt
     9961 cccttactcg agcagaagca gcaccttgac ctccttcaac agcgatgaaa gcttttgtca
    10021 tcgcaacaga aattttactg ggttcaaatg ccacaacagc gccattgcgc cggattattt
    10081 tataaagaga aagttgtaca ccttgattgg aagaaccttc agaaaactca ccatgaggaa
    10141 tcgggtttaa gaccgtgctt gtactattag ccgtaagctg catattcgaa ttctcctttg
    10201 agtcgcagat agagagacat actgaccgct ttgcttatca aaaacccagg tatgtactat
    10261 atatagtatg tgactggtaa tttatgattt acctattgta ctcaaaaaag ttattcgtat
    10321 tcaataaatt ttttgatcaa gcaaaaaaat tgctgatttt agattgctag agatttagaa
    10381 acgcagtgcc tagaataaat aaagtagaaa ttattacttt aggcaagaaa tgaaaacaac
    10441 aaaaataaaa accgcctcaa gggcggttta ggttacttca taacttcctt ttgcttcagg
    10501 aagctaagta atcactgcat taatgaccag gtttgccttt gccaccatat gggcgaggat
    10561 cttcaccttc tttcaggcaa ttaccattcg catcaatccc aaattgacaa tcaagacttt
    10621 gcccagaaat gattttttca taagcttctt tttcagtcgt aggaagagct tggcttggtt
    10681 tgccaccaca tccggctaac gataaacctg ctaaggccgc agcgataata atgtatttca
    10741 tccttaattt ccttatttta aataatgtaa caaaaagtct tgtgacggct tcagtatata
    10801 acgaaaacta ctttagactc aactaccaaa aaatcaaatt cttagaatct gtttttgtaa
    10861 atcgataaaa ggtttaagta gaaggggcag cgcttattca tgccctgttg gaaaaaccgg
    10921 taattgcttg aaaaaaacca tatcgtgatt aggccacaat tgtgcttgct cagaaatagc
    10981 gatagatttg agcttgcgta tactggctaa ggccagttca aaattatctt gccagcaaca
    11041 acccggcgca atttcatcgg ttaaattttc attcaaatcg gctgcatcac cgcatagaat
    11101 gataggttga ccttggggta actcaatcat caacgacaag tgtcccgcag tatggccagg
    11161 tgttgttaat acttgcacac caggtgctaa ttcatattct tcatgctgta atcgccatcg
    11221 atcagattgc aataactcat cgggaaaaac gcctgaatct tgtttggttc gggctgctgc
    11281 aagttcagct tggtgaagat gcacttcgca gccgggtaaa tcgcataaac cgccaacatg
    11341 atcaaaatgg agatgcccaa tcaaaatgag atcaatatca gcagtcgtta atcccaatcg
    11401 ttcaaggtag tggggaattc gttgttctgc ttgcatttga ggggtttcta taactggata
    11461 catactcgca aaaaactgct gacgcagagc aggatcagaa agcttgtgat aatcacaacc
    11521 tacatcatac aaaatgcgcc cctgagtagt ttcgatcaaa taagctagaa tgggtgcatc
    11581 aatccactgc ccgtggcccc ggttgtgggt tgaaatcgtt ttttcataac gaatcgttcc
    11641 ggtcaaaagg ggccataatt ttaaagcatg tgtcatcttg tcagaattgg aattaacctt
    11701 cgggcattct accaatttct agctatggag ttgtttgtgt catttgaatc acgggttgag
    11761 cacctgattc agaaagcaga accgtcatca tgttggttac gagcacagca cgttgatccg
    11821 aagtcagaga gacaatttgc ttttcttcaa gattcttcaa tgccacctca accattccaa
    11881 cggcgccatc cactaacttc tgacgtgcca tcacaaccgc ttctgcttgc tgtcgccgca
    11941 acatggcatt ggcgatttca ggggcgtaag caagatgagc aatcttggct tcttcaataa
    12001 cgattccagc cacgttgaca tgctcttgaa tcgattctcg taataaaccc gcgatggcgt
    12061 ctaagtctgt tcgcagactt tttttacgct taccgtcgat catttcagta tcgtcatagg
    12121 catggctact tgcaacttgg cgaacagcag actcgctttg gatttgcaga taattcaacg
    12181 tgctttcgac atcaaaaata gcgcgtgcag tatcctggac acgccacgcg atgacagccg
    12241 cgatctcaat gggactgccg cgttcatcat taactttaag aacaggggtg ttccaattat
    12301 ttattctgag cgaaacccgc gttttattat aaaaaggatt gacccagaaa aaaccgcttt
    12361 cattcacggt tccaacataa ttgccaaaaa ataccatgac ggcagcttga ttaggctcta
    12421 atgtaaataa tcctttgcat ccaaacaaag cgagcattgc taacaaaatt ccaccaaaca
    12481 attttatggg agggcctggt gtcatggtaa agaaaagact aaaagcaaat agagcgagta
    12541 gtgacactaa cattacccag ccatttgaac ctgatatgat tctctcatcc gtgctgatcg
    12601 aactactatt tgattgctga ctcacccttc ttctccttat ggataaacga aacttgaaaa
    12661 gtatacgatg atcgggagtt aattaattgg aagataaatc gagctatcac ccaattaata
    12721 ttattcttgc tatgcttttg tttttaattg tactcatatt aagaagaccc accctctaaa
    12781 caggagaaac gatgccattt agttttagta atcaacttag cgccattatt ggctatgctg
    12841 aacaacaaaa gccactatca attctggata tcggggttgg catgggtcag tatggttttc
    12901 tactgcgcac caatctggaa aatatcaatc tttttgaaat caatggtgcg aatgctcgat
    12961 tgcgcgataa aaatcagtgg agaattaaaa ttgatggtat cgaaggttat gcgggttatc
    13021 tcacgcctgt gcataattat gcctacaacc gcattctaat tggtgacgcg ctggagttac
    13081 ttccaacact cgccagcaat agctatgact tagttcttgc aatcgatatc ttggaacatt
    13141 tttataagga gcaaggtgtt catttcttaa aagaatgtcg acgtatcgcg aagggttcat
    13201 cgctcattag cacgcctaag gaattcatgg agcaggatgt tcctgcaaat cctttcgaga
    13261 atcatcggtc cgtctggaac gatgaagatt tgaaacgatg tggttttgat acgttcctgc
    13321 ctaatccatt cagttggatt gcgcttgcca gtcatcatga atgaggactc aattgctaag
    13381 acgcactgaa atttctcatc cagtattata ataaccaaga attcagcgat gagttgatta
    13441 ttcatatatt agcgaatgga ttatatggga gtacttatgt taaggattaa attattaggt
    13501 tgtttcattg caatcgtcag catgttattt tctttttcag catttgccta taaagagcac
    13561 tcagcagagc cgcatagcgc agcttctggt gtaagtgcat tacaaaaaat cgataccacg
    13621 gttggtaacg gggaagaagc catcgtcggt aaaacagtca aagttcacta tagcggttgg
    13681 ctctatgatg aaagtgcacc cgataaaaaa ggaaagaaat ttgatagttc ttatgatcgc
    13741 aaagagcctt tcgcatttat gctcggggca ggtcgagtta tcaaaggctg ggaccaaggt
    13801 gtaataggta tgaaagtagg cgggcagcgt accttaatta ttccatcgag tatggcttat
    13861 ggcgcgcagg gcgctggtaa tgtgattcca ccacatgcaa cgcttatttt tgatgttgag
    13921 ctggttggtt ttgagtaact aaggcattca agctagccgt tcaaaagcgg ctagtaagtc
    13981 actatcaagc aaaaaatgaa gggaatggct gatttctatg gaaacagaca tatcatcgcc
    14041 tacaaaaaaa cgtttcctga cacgtaagca ccttgcaatc agcttaggcg tcattttctc
    14101 gattgtagtg ctcttcgggg tattgggata tttctggtta cctggatttg cgaaagccaa
    14161 gctcgagcaa gttttgtcgg aaacgctagc aaggcctgtc accgtccaat cgatcgatat
    14221 taaaccgttt accctggaag ttgcagtgag aggcttccga atcggggaaa aatcaacggt
    14281 aagtgatcaa actacttttt tctcgtttga tcaattatat gtagatctta gtgctgaatc
    14341 actcatccat ctggcacccg ttgtgacaac tatttcatta gttgccccga aaatatatgt
    14401 cacaagggag gataaaaacc agttcaattt ctcagattta atcgagaaat ttgggcaaac
    14461 gcctgaagaa aaaccacaag agcccaaaaa accaacgcta ttttcaatca acaacatcag
    14521 tatccaaaac ggtgagatta cctttgccga tcgaatcaag aatagtcagc agcacattac
    14581 tgcgatcaat ttgagtattc cattcattgc caattttaaa aatgtcctca caaattgggt
    14641 ggagccgaac ctgagcgcca aaatcaacga ggcgcctgtt accttgtctg gcaaagtact
    14701 acctttttcc gacaagcaag aagcaacgct ttcgctcaaa ctggatgata ttgatttgac
    14761 gaatatcgat gaatattcac cgattccgtt agggatccgg ctactatcag gaaaatttga
    14821 tagcgatttg ttagttagct ttacccatgt aatcgatgag ccacccaata tcgatctatc
    14881 cggtcaaatt gcgcttaaag gaatacagat tgaaaataga acggttgaaa tgccttatat
    14941 attgggtgtt aagcaattca acctcaagtt gaatgaagta tcattcaatg aaacaaagcc
    15001 tgtgaaagtt ggaacgactt ttgagtccat tgcgataacc ccgattggag aaaagcaagc
    15061 tttactatcc ttaccaaaaa ttgaattaga tagcatcacg gtcgatcgcg cacggcacaa
    15121 aatcgacctc ggtaccatat tgttagatgg gattaatggg ttaatcaaac ggcaacccga
    15181 tggtcagctg gaattaaacc gcatgcttgc ggtaactgca tcatccaatt caatgcagtc
    15241 ggaagaagat aaagtaaaga aactaagcaa gccgatccag cgcaacgttt cagcgatcat
    15301 tccgattccc gacaaaaaac cagataaccg cgtaacgtct ttagcacaaa cacaagcgcg
    15361 tgaacaggag ctcgtggctc catcagttat aaccattcca ttgcccgaac ataaaccgca
    15421 gattgttgct agaccagaag ataccaagca accacaggct aacatgggaa aaatggctga
    15481 ggaaacatca agtcagtctg ccccagcggc tgaagaaaca gaccagccgt gggcaattta
    15541 tatcgatcag atcaaattga gcgatggcgc tatcacgttt gaagattaca cggtatccaa
    15601 agttgtacca atgcatatca atccgcttaa tctcacagtg cgcaatatcg acctaagcgg
    15661 ggcgcagcct gtggagctat cgctccaggc gcaagttaat caacagggga gtgttgaaac
    15721 gaaaggaacg ctcgcttggg caccactcgc tgctgatctt gtgttggatc tcaaacaagt
    15781 cgatctagtg ccattgcaag gttggttcgg caatcagctc aatatactgc tttcaaaagg
    15841 taatttttcg tttcaaggcg atgttaaagc tgatggggaa ccattaaaaa ttgctttgca
    15901 gggtaaagcg cagctcgcga attttaatgt gctcgataaa gtcaaacttg ctgagcagtt
    15961 acgttggaaa agtatcgatg tgaccggcat tcaatttatc aacgagcctt tgcaaattgg
    16021 aatgactacg atcgatttaa cggatctttt tgcgcgcgtc attcttttag ccgatggaaa
    16081 gattaatctg cgagatctca ccgaggcaaa taaagctgaa gccagtccag taaaacctga
    16141 atccacacca gttgcgagtg caccaaatca ggcagaagca cagggtgctg ctaacaataa
    16201 agctcctttg ccgatcaaaa ttgataaagt caccttaaaa aatacccata tcaactttac
    16261 cgatcaattc atcaaaccta attaccgagt aaaccttacc gagcttaaag gtcaagtcag
    16321 cccactccat cccggaaaag ctggaaaagt cgatattcga ggtacgatcg acaaatcagc
    16381 accgctgcaa atctcaggaa ctatcgatcc ttttagtgaa cagctttcct ttgatatcgc
    16441 gacaacggtg aaagggatcg atttaccaac tttcagccct tattcgggta aatacatcgg
    16501 acatctcatt gaaaagggca agctttcggt tgatgtcaat tatcaaattc agcaaggtca
    16561 attaagtgct gagaataaga tatttctcga tcaactaaag attggcgaga aagtcgatag
    16621 ccctgatgcc gtttcattgc ctttggattt agcgatatcc ctattaaaaa atcgcaaagg
    16681 ggaaatcaat ctgcgcttcc cagtcagtgg ctcgatcaat gatcctcaat tcagcatcag
    16741 cggagtagtt tgggaggcct ttatcaactt gatatcgaaa gccttgacgg caccctttgc
    16801 cttattgggt tcttttttag gtgatggaga ggaattagcg gaaatcacct ttccttcagg
    16861 ctatgccacg cttgaacctg aagcaatcaa tcacctccag agtttggccg atgctttaat
    16921 cgatcgaccc gagctcaagc tcgaaatcac gggctatgct gaccccctca acgattatga
    16981 aaccctcaag caagtattgc tcgatcgtaa agtcaaaaca caaaaacttt cagaaacggt
    17041 taataaaggg caatccgtcg atctactcga tgaaatcaaa ctagacgatg ctgaatacgc
    17101 acgttaccta gaaatcgttt acaaagcatc tgattttgag aaacccaaaa atatgattgg
    17161 attaagcaaa agcttgccag tcgatgaaat gaagcaactc attttaacga ataccaaggt
    17221 gagtgatgaa gcgctcaatg agttagcaga gaaaagggca agcgtagtat ttcattggct
    17281 cattgagcaa ggtggtgtgc cgagtgaacg ggtttttgtg ctcggaactc atttagaatc
    17341 tgcaagtgct gctgagaaac cttatagcaa agctgcattc gcgctcaaat aatctcccgg
    17401 taatcactta tccatcctgc aaggcatgcc taattttgca aacctgctca ttcgctggca
    17461 acagcagcat ggtaggcatg acctaccatg gcaaggcact cagaaccctt atctgatatg
    17521 ggtcgcagaa attatgctac agcagactca ggtaaaaacg gttattccat tttatcaacg
    17581 ttttatagaa acttttccta ccattgctgc gcttgccaaa gcagacctgg atgatgtgct
    17641 agctatgtgg agcggccttg gctattacgc acgcgggcgc aatcttcata aagctgcggg
    17701 tgtcatcatg aatgattatc aagggaaatt tccaacgtgt tcgtccgaaa tacaaaaatt
    17761 gccaggtatc ggtcgaacga cagccgccgc gattgcagca ttttgttatg gcgaaccctg
    17821 caccatcctc gatggtaacg tcaaacgcgt acttgcacgt tattttggta tcgaaggtta
    17881 tcctggatta aacgccacgc aacagcggct ttgggaaaaa gctgaagccg ttctgccgcg
    17941 tggtaatact caaaaaaata tgccagctta tactcaggcg ctcatggatt tgggcgctat
    18001 catctgcacg cgcacttccc cacgttgtac agcttgccct ttgcaatccc attgcaccgc
    18061 atttctggaa aatcgaatct cttgtctgcc aacgcctaaa ccttccaaag ctttaccaag
    18121 caaagaaaca gtttttctat tgctgctgca ccatgacaag ctattgctag aaaaacgagt
    18181 gaatcaaggt atttggaagg aattatggtg tttgcccgag gcagaaaaga atgatcacta
    18241 tcaaaactac tgcgcgcaaa tgctaggaat cgatgcagta tgtgtcgatt tattgttacc
    18301 accgattgat cacacgttca cccattttaa gctcaggatg tatacgcaac ggctgcaagt
    18361 caaatccatt attcagcaac cgcaatgcaa tccaatacgc tgggttacaa ccgatgaagc
    18421 atttcaattg gccataccga ctgcgattaa aaagattctg tcaaaatacc gtgcagcatc
    18481 aacaccaaca tccctttaga tcaagactat gaatgattgg caaaactggc ggaaacagca
    18541 acgtgctgag cttattgcac aacgaatgca aataagtagc agcgaccgta tcctttggaa
    18601 tcaggcgatt actcatttcc taatacaagg tttcccaatt ttgcaacaac acgcagttgg
    18661 cttttattca gcctacaaag gcgaatatga tcccatgccc gtcatggaac atctaaatgc
    18721 ttgtggcact accctcgcac ttcctgaagt gatcgagtca ggtcagcccc tcatttacag
    18781 aaaatggtgg ccacaggttt ccatcaaaaa agatcggtac ggtttatctg ttccctgcga
    18841 tactgaagca gtgctgatta gtgctgtgat gataccgatg gtggggtttg acgatcaagg
    18901 ctatcgctta ggctatggca gtggttattt tgatcgtact ctcgctgcta tgacaactca
    18961 cccgttaacg attggcattg cgttcgaaca acagcgaatt gccacgactt atccgcaatc
    19021 ccacgatatt ccgatggatt ttatcgtaac cgaaagatcg atctatgccc tgaatcagcg
    19081 attgcatcct ttgtcgatag aaaactgtgc gcgtgttagt acgcagtctc aaagttagcc
    19141 tccagaatcc acagttataa accacaaact tagaaattaa ctagtaaatc tgtacgtgtt
    19201 attttgaaga aaaattgaaa tttctcagct gaatatttct tgctctatcg tacgcattga
    19261 cgtacgatta tattcataaa aaatgaggaa gctatcatga ccatactcaa agctactgag
    19321 gcccgcactc ggctttacaa cttaattgat gaagcggctg aaacgcatca accaattgtt
    19381 attacgggca aaagaaataa tgcggtattg atatctgaaa acgattggaa cgcgatcaac
    19441 gaaacactgt atctagtatc gattcccggc atgcgatcgt ctattcgaga gggcatggaa
    19501 tccaatttat cggagtgctc caaggaaatc gactggtgaa ctgggaaatc tattacacca
    19561 aacaggctca gaaagatgca ctgaaaatta gtcaggctgg attgaaacct aaagttgtag
    19621 agctattgaa attactcgtt gaaaatccgc tccagaatcc cccctcctat gaaaagctgg
    19681 ttggagatct aacgggtgca tattcgagaa gaattaccat tcaacaccgg ctgatatacc
    19741 agatatatcc agaagataag aagatcaaag tgttgcgaat gtggacgcat tatgaataaa
    19801 aacttgattc aacaagacta agtttattga tatgattatc cgcagcattt aacacaactg
    19861 atcgataact tagcctttag aatcaccaaa agtaagtagc aaaatataat gaaaaaatcc
    19921 ggcaagaaga atcctacgtg gaatgacctc aaacgacaac ttgcaaattt cgaccacaca
    19981 ggactcctct gtctgattca ggatctctac gttgctgaca agcagaatca ggcttttcta
    20041 catgctcgct ttggcttggt gacgatgcgc tcgcaccgta caaaaccatt attgattgct
    20101 ggatttgtcc cgatgtgatg cgcaatcaag atatatcggt tgccaaagcc aagaaagcgg
    20161 tctccgatta caaaaaagcc atcgggcgac cagaggggat cgccgagctg acagtatttt
    20221 attgcgaatc ttgcatggat tttctcggct actgtggaat ggatgacgaa ggctatttca
    20281 acgccttgat tctcatgttt gagcaggctt taaaagcgat tcaaaatctt gatgtcaaac
    20341 agcaagcggg ttttgttgaa cgactcaatg ttcttcagca cgcagggcac aactggggca
    20401 tgggagatga aatagaggac ttgatggtga agtatggatt taccaaaacg taaatggagt
    20461 ttggagttgt cccatacagt ttgattattt tcattgtgtt ttttatagaa attcataaaa
    20521 aattgagtaa atcaaactgg aatcgcacta tctttgctcg attttatgaa gtcaggtgtt
    20581 ttgcaaaatt ctctttatgt taatacaaga ccagacccca tttatttgtg gacagttttt
    20641 ttgcttactg cgctaatatg attaactatt gtaacgctaa tatttttgtg ctgatctgca
    20701 gcaataggat caatggacct gagattttat tgatttctgg atcatatccc atcgcgagac
    20761 cggtactatt ttataattaa ctgtcaaata gtagattttc agtttttgta gaccattaca
    20821 cttatcttat tgaatgatct ggtaccggga atgatagttc aacaggagaa tttgcagctt
    20881 catagcccat ttgtattcct tcctcaattt catcccacct atcaaatcca agtgcatcaa
    20941 ctaagtcttt ctgtggggca atgtagttta ttgagcatcc ttcatcttga aaaggagtac
    21001 cataattttt tgcaagatct cccccaatcc atgaacttcc gccaagtgta cgctccagta
    21061 taggcccttt ctttgcccaa tattcattaa attccgctac tatttgccgc cgcttgtcat
    21121 tatccataag tgagaaacat cgataaagcc agcccaaaaa ctcattttgt gcgatggaaa
    21181 ccatcacatc gttacgagct gactcattaa accagacatt cagcaagcca aataaatatt
    21241 gtgcaacagg tagggctgag aaatctactt taccttcttc gctatttatt aagcctggat
    21301 ttttgaaatt ccaatgtgct gcctgcaagc ggtctcctgt aattacaact atttcccgag
    21361 cacctagacg tatagctgtt cggatcgcca ttgtatcgcg tagtccacca tcaaataatg
    21421 gatattgtga ttgcctagaa tcacctttaa aaacatcatc actctccttt aatatttgat
    21481 caagattata aggaggtgca aaagaattct taagatcctt gaaaacttga ctgccaaatg
    21541 gttgttctcc acgtgtaaat tgcattatcc gctctattgc aggtaaagga taagtagcgt
    21601 tgaaggcagt gccgggtacc ttaagcatta caattttttc attcccatat gaaataggcc
    21661 gtgcagtgcg aagattaacg attttgggat ccataaaaac tggtaataca gatgaagaat
    21721 aaactgcatc ctctattgac atcgcgtaag catcagcacc aaaaatcgga cgagttaacc
    21781 aagtctcacc catacgatgg tccggttcta actcaattcg cccacacata tttagcccca
    21841 atgctccggc ttcaaaaggt tcagttactg aaaaatattg accagttctc agatcagtta
    21901 taccaatacg taattgtgtg cccccagtcc taatcgcgcc aatgtttaaa ttccgatgaa
    21961 tcagatggcg taaaggatgc attgaatgaa ttgcatgcaa atgactggca gcatacttaa
    22021 gccattcaaa gatatcttta gcaccaataa gatcgtcaat tccatcagat agcatattgg
    22081 gtatccaagt tgacaagaga ttgacaaaat agtcttttga gtaaacagca cgccctcctc
    22141 gcttctctgg tcgaagttct cgccaaattt cctctagttc tgctggtgaa gtatccttcc
    22201 gttccgcaag ttttgccgca tttaaagctc caactgaaac tccacaaata atatctggcc
    22261 tccaaccttt actccaaagt tgctttacag ctccaacttc aaaacaacct ttggcgccac
    22321 caccacttag aatcaatgct tttttgttgg caccgcgtct acggcgaggc gagttgatat
    22381 taatattaat aagtccttct cgattgatat cgctattatc aatattctga ttgccaactc
    22441 cctctattga atcaaagtta ggctgtatcg taccaggtgt tagaatagat acagaaggga
    22501 taattaccga gaggggtgga ataatataac cttcatcccg aacaaattca taacctgcat
    22561 ttgccgcatc ttgttcttct ttaatactag tagttgtaaa tgagtctttc cgttcagcac
    22621 ctttccaata catacgaagc ggaatagttc ccggttctgg ctttgactta atccaacctt
    22681 caatccttac cttaacataa ccattatcat tcgcctcctt ttctccctct ttcgttgctg
    22741 taataaggtt atctccattt tgattattcc agaaaagaaa tagtgggtta caacctggca
    22801 tagaattcat ataaactaag ccttgtgtgc gaacaaatgc atatcctgct tgtattgcct
    22861 gtaactcacc aagtggtgtt gctgtggaaa aattatcagc tcgttgatcg ctccaaaata
    22921 gcttcagctc actcattgtt tcaactacaa aaatttcctg tgaccctgga ttatctttat
    22981 tgataattct caacagccca gaagaatctg acgataaaaa accaggtttg tcagtttcaa
    23041 caaactttaa agcaatctca tcaccatcca aaagtacctc accatcacga tttaatctag
    23101 taatgatgaa atcatcccct ttttcaccaa aaaaatgagt ttgtacaaag agtctatttt
    23161 ggtgacgccc cctcgaatca atagtagtga cataagcatc ttggcggtaa ttttgactca
    23221 tatggaatgt agttaaatta cgagtggtgc gaagtgttcc tctatcacca ctcgaaatac
    23281 gaagtgtacc tctatgatca cttgaaataa cttggttatt tatgactagc gaaaactctt
    23341 ctgtttcccc tttattactc tttccagcat tcaggggagt tgtatcagat gcaccatcac
    23401 agcttaaata ttttccactt gaggttctca aacgaagttt catattttta atctcccaca
    23461 atgagcagtc tttaaatagc cactgataat attcggaatt tagctataaa acccagattt
    23521 aaatcaaatc agaataaggt tttttaacaa tgctacctga tcaggtatct ggttcttcag
    23581 ctattacaat ttatacttat aaaataatct aatcccagga ataattctcc tactgagcgc
    23641 tactcaaatt ggtagtaaac gctactcttg atcatttata atttaatagg ggtatgtttt
    23701 aaatgaacgg taagtctcct gattggatat cattgatcga aacgagctat agcctggagg
    23761 gtaatgactc gcaatggttg agaggattaa ttgaccatgt atcgaaaata ttaaaccgtg
    23821 gatcggaacc ggtgggttgg acatttcatt gcacaccgac caaattcaag ctaggcagtt
    23881 tttcggaagg aacctctaaa gcattaactt acgctgcgcg tatgtcgcat gcgttatcga
    23941 ctgagaaatc cttggatcta acctatcgca ccggtgtcgt cattgcgacc gcaagtgaat
    24001 tggt
//
LOCUS       DAOQKQ010000015        22980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_948_length_22980_cov_5.797906, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000015 DAOQKQ010000000
VERSION     DAOQKQ010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 22980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22980
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_948_length_22980_cov_5.797906"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="PKZ49_01890"
     CDS             <1..596
                     /locus_tag="PKZ49_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35342.1"
                     /translation="ILSIVAIVSRRRLFSSSSAIGLLVIASASCAVYYQWLNPPKLAF
                     SEWLHTEVAAYGLLCTAFWVSSGRPMPSRFTSHSLAPILLITGIVLHWWSTPLVIQQL
                     IGVLCFVGAICVLSTTKGWFASALSWSPLRKLGLWSYSLYLWQQPFYLWSHSEQGPSA
                     WIALCAALFCGLISYYFVEQPVRIYLNTNWKTRRRDS"
     gene            814..1263
                     /gene="aroQ"
                     /locus_tag="PKZ49_01895"
     CDS             814..1263
                     /gene="aroQ"
                     /locus_tag="PKZ49_01895"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HND35343.1"
                     /translation="MQKNITAKNILVIHGPNLNLLGIREPDIYGTVTLEVINHKLLEM
                     AQAAGIQLNIFQNNSEGLLIDRIHSTFQDGTEAIIINPAGLTHTSVALRDALAATNLP
                     FVEVHLSNIYTRESFRHKSYFSDLAVGIISGLGHKGYEFALQFFIKS"
     gene            1411..1890
                     /gene="accB"
                     /locus_tag="PKZ49_01900"
     CDS             1411..1890
                     /gene="accB"
                     /locus_tag="PKZ49_01900"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HND35344.1"
                     /translation="MDLRKLKKLFELVDEHNITELEITEGEEKVRISKNSNQIIQGYT
                     IPPQQSHFSYSGVAEASQKQTVNESVDSDDKPKTELPDGHIVTSPMVGTFYRSSAPGA
                     KPFVETGHSVKVGDTLCIIEAMKLLNEIDADKDGIIKSILVENGQPVEYGQPLFVIG"
     gene            1952..3319
                     /gene="accC"
                     /locus_tag="PKZ49_01905"
     CDS             1952..3319
                     /gene="accC"
                     /locus_tag="PKZ49_01905"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HND35345.1"
                     /translation="MFEKILIANRGEIALRIQRACRAMGIKTVAVHSEADADAKYVKL
                     SDESVCIGPAPSAQSYLNIPAIISAAEVTDAEAIHPGYGFLSENADFAQRVEESGFAF
                     IGPRPETIRLMGDKVSAKNAMRQAGVPCVPGSDGALPESLDEIKKIAKSIGYPIIIKA
                     AGGGGGRGMRVVHTEAALSNALITTRNEAQAAFSNPTIYAEKYLENPRHIEFQVLVDS
                     YGNAVHLGERDCSMQRRHQKILEEAPAVKISSRLRDKIGERCTEACRRINYRGVGTFE
                     FLFENNEFYFIEMNTRLQVEHPVTETVTGIDLVRAQIKIAAGEKLELKQKDIIFQGHA
                     IECRINAEDPYKLTPSAGRILQYHSPGGPGIRVDTHVYHNYMVPPYYDSMIGKIIAYG
                     DDREQAIARMRIALSEMVIHGIKTNIPLHLDLLSDSHFLDGGTSIHYLEHKLALHNKS
                     DKGDR"
     gene            3338..4261
                     /gene="prmA"
                     /locus_tag="PKZ49_01910"
     CDS             3338..4261
                     /gene="prmA"
                     /locus_tag="PKZ49_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HND35346.1"
                     /translation="MYWNSLTLDVDAAQVELVSEKCFALGALSIDIRDAAADTADEKP
                     LFGEPDVIHDEIWDHTFVSALFEHHVDIAIILQEIKQTLNLPYPPKYALAQVHEQDWV
                     KLTQSQFDPIKISQRLWIVPTWHTPVDTNAINLILDPGRAFGTGSHPTTQLCLSWLDQ
                     QFQPGDAVLDYGCGSGILAIAALKLGASRVIGVDIDANAIQASQENAMINQCNPQSIL
                     FSTSLNLPIANFFEKRYVDKVVANILANPLILLAPLLANAVREGGEIALSGILREQAD
                     EVCQCYRQWFDIDIVDQKDDWVLLVGTKYLD"
     gene            4275..5075
                     /locus_tag="PKZ49_01915"
     CDS             4275..5075
                     /locus_tag="PKZ49_01915"
                     /inference="COORDINATES: protein motif:HMM:NF023334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon and DUF3426 domain-containing
                     protein"
                     /protein_id="HND35347.1"
                     /translation="MVLVTKCPVCQSVYRVTFPELHLHDGKVRCGQCEQAFNAFRALI
                     SIPESLIRMEDSQLALPVHEEVELPAINKHPAPSGLQSSEQAELTSASIADQADQNGV
                     AEVHTPNLIWKQVAASLALIILLCVQLIYFYRTELAIAIPSSRAILEKSCDLLQCEVH
                     LPKYSALLSLESSELRVNPNHQAEIVTLLATIRNHAPFQQAWPELRLTLTDLDDQPIA
                     SRILLASDYLNDNKLPQFFAPDSEIEIKIYFDNSELKAMGYRLELIYL"
     gene            complement(5120..5512)
                     /locus_tag="PKZ49_01920"
     CDS             complement(5120..5512)
                     /locus_tag="PKZ49_01920"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HND35348.1"
                     /translation="MAIEISWEAASNKTACQIQENGKTWISDIGLESGGSGQYPDPHQ
                     MLDSALGACTVLTIQIYAKRKAIPLERVLVSITHTEEAGLYQLQRNIQLKGQLTEAQR
                     DDLLRVANKCPIHKVLSGQFTIITQLEN"
     gene            complement(5642..7411)
                     /locus_tag="PKZ49_01925"
     CDS             complement(5642..7411)
                     /locus_tag="PKZ49_01925"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HND35349.1"
                     /translation="MLKYDYSLSKNEQIALFFFIIGLITVFGLLLFSNKSEERIAESS
                     THLQQDLTKQFYQQKLQQREAEILEVLKHTDPKHRRILEVELQDIQLLLQDIDNSFLL
                     LKTSINDRLKQLEVLHQAYPIQMLEQAIMALRQANDQQAIQLFRQIEKNNEHNPPLAA
                     EAAYQLSKLAEDSVRYTAGLEHAEKAIRLIPNNALYLSAAGFFSDKLAHYKKAISYHE
                     QALAILRTLHSENHPEIAAVKNDLGMAWDALGEYAKAIKFFEQALESLIQLHGENHPD
                     VAAIQNNIGMAWDSLGDYPRAIRYYEQSLEADLSHYGEDHPSIAKRRNQIDHAWESIE
                     AYHDAIQYFEAALDSDLKTYKTNHPSIARDRNDLGIAWESLGEYHKAIEYFEQALETS
                     VAIYGKHHPLIVTYLNNLGNAWDSLGKYRKAIEYFEQALETGLALHDENHPSIAVTRN
                     NLGNAWESLGELHKAISYYEQALAIDLKAYGQNHPNIARDKNNLGNAWESLGEHQKAI
                     YYFDQALSIVLQSHGENHPQVTTYRNNLGWAWYSSGHYQNAIDYYKQALVTAKKLLGE
                     DHPNTKVIQSNLRSAESKLIATP"
     gene            8170..9279
                     /gene="ald"
                     /locus_tag="PKZ49_01930"
     CDS             8170..9279
                     /gene="ald"
                     /locus_tag="PKZ49_01930"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HND35350.1"
                     /translation="MIIGVPKETKDHEFRVGLTPGTVHALTTQGHHIKIESGAGLGSA
                     FTDDDYRLAGAEIVTYPRDAWAAQLVIKVKEPTESEYAYLRQDLILFTYLHLASNKPL
                     TEQLLATGTTGIAYETVQLASGELPLLTPMSEVAGRMAAQIGALYLQKTLGGRGILMG
                     GVPGVAPANVVILGAGIVGVNAARVTVGMGAHVTILDINHSRLKYLDDLFRGQLFTRM
                     NDSFNLNELVYQADLVIGAVLIPGGRAPWLITREMLTKMRKGAVIVDVAVDQGGCVET
                     THPTTHSNPVYEVDGILHYCVTNMPGAVPRTSTLALNSQTATYIKLLADSGLDAVRNN
                     NALQKGLNTYQGLITHTAVAEAFGLQSTAPLQALA"
     gene            9489..10442
                     /locus_tag="PKZ49_01935"
     CDS             9489..10442
                     /locus_tag="PKZ49_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HND35351.1"
                     /translation="MASPLHAQEVVVYSARMEQLIKPMFDAFTTETGIKVKFTTDKEG
                     ALLARLQAEGKRTPADILITSDAGNLWEAARAGLLKSISSPILEANIPVQLRDPQGQW
                     YGLSIRARTLVYNKEKVDPSELSTYEDLANPKWKGRLCLRSSKKVYNQSLVAMMIAEH
                     GEAKTEQIVKGWVANLATEPLSDDTKAMEYVAAGRCDVTLVNSYYYGRLMETNPNLPL
                     VIYWPNQDNNGTHINISGAGVTQHSKHEAAAIKLLEFLSSEKAQNLFADVNFEFPANP
                     QIKPSPIVTSWGDFKKSPMSMIKAGELQATAVKLMDRAGYR"
     gene            10538..12136
                     /locus_tag="PKZ49_01940"
     CDS             10538..12136
                     /locus_tag="PKZ49_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HND35352.1"
                     /translation="MNFWRLAPFCVASIVLIPIIVVISSIFTPADDIWHHLAETTLPS
                     VLLNTFWLCLGVVLGAIILGTSLAWLTAIYDFPGRKFFSWALLLPMAIPAYVTAFVVL
                     GLFDYTGPIQTYLRSWTGAESNWIPDFYGRSGAILVMVLAFYPYIYLLARNAFITQGK
                     RLIEAAQSLGLSRKQGFFKVALPMARPWIIGGAMLTLMETLADFGTVSVFNYDTFTTA
                     IYKAWFSLFSLSAASQLASLLIILVFIIIIIEQQLRARMRFAESKSSRTTFRIPLLGW
                     KRLAACSYAFSILFIAFILPCSQLLIWAIDEINKNIDSRYIEFLIHSLTLSTIATLII
                     GSVAILLVYATRLYSDKATHIAVRIATLGYALPGAVLAVGIFISLAWIDTQLNQIILI
                     LFEIEAGFLIQGTIIAMLIAYLVRFLAVGHYPIDSAMQRVTRSIDEAARGFGLTGWSL
                     LSRIHLPILSSGIFTAATLVFVDVMKEMPITLMTRPFGWDTLAIRIFTLTSEGAWNQA
                     ALPAIALVLAGLIPIILLMRQTEQ"
     gene            12142..13212
                     /locus_tag="PKZ49_01945"
     CDS             12142..13212
                     /locus_tag="PKZ49_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HND35353.1"
                     /translation="MESLLKLTQIHQSYHQQEIIKHLSLELKKGEIGCLLGPSGCGKT
                     TVLRCIAGFESISSGEITINGKIVCNADFNLPPEQRRIGMVFQDYALFPHLSVSANIG
                     FGLQQYTKAERTHRINELITTVGLTEASDRYPHELSGGQQQRVALARALAPRPDLLLL
                     DEPFSNLDISLRERLSLEVRDILKDQHTTAILVTHDQTEAFSVADVIGVMHQGEILQW
                     DTAYNLYHHPTNRFVAQFIGQGTFVPGHLISPEKIQIALGIVENPDYSDYFEQSEHTT
                     NEAVDVLLRPDDIVYNEASALQATVVNKAFRGANILYTLKLSSGQTLLSLIPSHHNHS
                     IGESIGIQLAADHVVVFSREKM"
     gene            complement(13246..13833)
                     /locus_tag="PKZ49_01950"
     CDS             complement(13246..13833)
                     /locus_tag="PKZ49_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HND35354.1"
                     /translation="MRNLVLIILLLKPCWIFAGAQLYEPLSASTRTVLQKAISDQPIA
                     QFVTLPDAESRRWLAVMSARMEKYMPDQNEREGFLTTVHYEAMRAGLDSQLVLSIIHV
                     ESRFRKYAISSAGARGYMQIMPFWIDTIGSKEHNLFHLRVNLRYGCTIFRHYLDLEKG
                     DYFRALGRYNGSLGSATYPDLVFSAWQKNWFFSKD"
     gene            complement(13821..15533)
                     /locus_tag="PKZ49_01955"
     CDS             complement(13821..15533)
                     /locus_tag="PKZ49_01955"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HND35355.1"
                     /translation="MRVSNFFLSTLKEAPAEAELISHKLMVRAGLIKRLGSGLYSWMP
                     LGLRVLRKVEQIVREEMNRSGAQELLMPAVQPAELWQETARWEQFGPQMLKIKDRHEN
                     DYCFGPTHEEVITDIVRREIKSYRQLPINFYQIQTKFRDEIRPRFGVMRAREFIMKDA
                     YSFHVDQASLEKTYQIMYETYSRIFTRIGLDFRAVAADTGAMGGSSSHEFHVLADSGE
                     DAIAFCPHSDYAANVELATSLPGNSMPSEAPTGAKNKISTPGIKTCQEVAEFLGIPIQ
                     KTVKALAITADEQFFLLLLRGDHQLNETKVRKIPLLTNFEFASEERIITETGCKPGYI
                     GPIGMEKYLIADRSVLEMSNFVCGANEENFHFTQVNFGQDLKVPDRCFDLRNVVAGDL
                     SPDGKGQLEICRGIEVGHIFQLRTKYSEKMKATFLDDAGQIKVMEMGCYGIGVSRVVA
                     AAIEQNHDAAGIIFPTAIAPFEVAIVPIGYHKNQSVQQAADQLYQTLSQAGVEVFLDD
                     RDERPGVMFADMELIGIPHRIVIGERGLKEGAAEYRGRRDQAAQMIPIAEADHFLRAK
                     VCGT"
     gene            15763..16329
                     /locus_tag="PKZ49_01960"
     CDS             15763..16329
                     /locus_tag="PKZ49_01960"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HND35356.1"
                     /translation="MIDRNGYRPNVGIILLNSKNEVFWGKRVKQNSWQFPQGGIKTGE
                     TPTQAMYRELTEEIGLQPNHVEILGRTREWLRYNVPECWIRRECRKSYKGQKQIWFLL
                     RLLGRDTNVSLRKSPHPEFDAWRWNQYWVELDSVVEFKRQVYRQALLELSRLLGRDIG
                     SAYDYGYIENAQSNTNKVLSNSTVTLKD"
     gene            16469..17470
                     /locus_tag="PKZ49_01965"
     CDS             16469..17470
                     /locus_tag="PKZ49_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HND35357.1"
                     /translation="MMMRKLTVSLLSIGVIMASMHVTAANEPIQPIKPVKPENAELVE
                     LGKMLFFDPRLSKSGFISCNSCHNLSMGGTDNIQTSIGHKWQQGPINAPTVLNASMNL
                     AQFWDGRAKDLKEQAGGPIANPGEMASTHDIAVKVINSMPQYQAQFKKAFGSDGVDID
                     RVTTAIAAFEETLVTPGSRFDKWLEGDKNALSKEEQDGYNVFKTSGCAKCHNGPAVGG
                     NSFEKMGVHKPYETKSTAEGRKGVTGKEEDNLHFKVPTLRNVELTYPYFHDGGAATLE
                     EAVDTMAKIQLNKELSKAEIANVVAFLKTLTGKQPDFKLPILPPSNNNTPRPEPFDK"
     gene            complement(17574..19622)
                     /locus_tag="PKZ49_01970"
     CDS             complement(17574..19622)
                     /locus_tag="PKZ49_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HND35358.1"
                     /translation="MIEFFSALVRWLQLTANLVLFGSCVFLTLIWFGKKLPELGWVRK
                     LEQLFPWLVGIVLLGLIGILASTAGQATGDAINTWDLNAWWEIVLKTRVGHLWVARFG
                     LALLLLFIVLRLKRMHRVRWHYGLLAVTAALPLTVGSLVSHAGAEEISFASVTPYTLH
                     ILLAGVWFGTLPAIIYIIRDALNNSQAGTLLLHADFLKQFSVLALPVMILLFLTGWMA
                     ADRMIGDYYHTLVSSRYGWLLNLKLSILGIILWIAYRIRSVWLPLFIQENVRAGVAIG
                     QLKKWVGIEFLLALILVLVATLLANTLPGKHAIIDHWPYPFRLSIDATWDEPGVQNFF
                     WAGTLLFILAIGIAAMGTLNRWNQSKRVILPVIMGLVSLGIALPPLIIEAYPETYQKT
                     PIPFDAISISKGATLFAEHCVSCHGPQGKGTGTAPELFVADPETGEFPPDPTDLLTKT
                     HTARHTVGNFYHWLSYGVEGTSMPGFSAVLTDENRWDVVNYLHALSRGYMGRLLGSLV
                     TPNQPFLAPPVFSYFAHDGSGGSLKDFREKSNVLLVLFSWPQSQQRLFQLSDIYEMLK
                     NDFHTEILLIPNRALTEEEMAAVTRIVPFPVITEGWSEIRDSYLLFRRTMAVPDLLRK
                     GMTPEHIEYMIDRFGYLRARWVSQFDGFGWLITGVLTKQLTILNQEEKIKPPPDDHVH
                     "
     gene            19894..21168
                     /locus_tag="PKZ49_01975"
     CDS             19894..21168
                     /locus_tag="PKZ49_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HND35359.1"
                     /translation="MSDPIITCPNCHAAIHLTESLAAPLLAATRQQFQQQLAQKDEAI
                     SQREQAIRQQEQLLLEAKRNVDQQITEQVAAQLKAERDHLIADEAKKAKLAVAVELEN
                     NLRELANLKELLQTREQKLSEAQAAQADLLKKQRELDDTKRELELTIEKRVQQNLTTI
                     RNQARQEAEESLKLRVMEKDQTIAAMHQKIEELKQKAEQGSQQLQGEVQELELEALLR
                     SKFPQDSIEPVPKGEFGGDILHAVISPSGLPSGLILWESKRTKNWSESWLSKLRDDQR
                     NAKAELSVLISQALPKGIETFDIVDGVWVVHPRATLPVATILRHMLLQMSRTKQVSEG
                     QQTKTELMYQYLTGPRFRQRVEAIVEAFTTMQTDLDKERKAILKQWAKREEQITHVME
                     ATVGLYGDLQGIAGKSLQEIEGLELEYLEIKD"
     gene            complement(21293..22096)
                     /locus_tag="PKZ49_01980"
     CDS             complement(21293..22096)
                     /locus_tag="PKZ49_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HND35360.1"
                     /translation="MVYVYPVISRRSGGVSIGINLNPNNACNWRCIYCQVPNLTRGSA
                     PKIDLPLLEQELRIFLRELLEGDFMAQQVPPECQRVNDIALSGNGEPTSAKEFCEVIE
                     VIARVKHDFALAESIKLVLITNGSLINRVAVQRGLARMATLNGEVWFKFDSAIPDRRQ
                     LINGASISNGTIKRHLKIATTLCPTWIQTCMLQIDNQPPCESEVDAYLEFIAQARDAQ
                     IPLQGVLLYGLARPPMQAEADRLSKVSDQWLAQLADRIKALGIEVKAYP"
     gene            22168..22623
                     /gene="queD"
                     /locus_tag="PKZ49_01985"
     CDS             22168..22623
                     /gene="queD"
                     /locus_tag="PKZ49_01985"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HND35361.1"
                     /translation="MLITRRLEFDAGHRISTHTSQCRHLHGHRYVIEVTLSGNIIAEE
                     GVPEQGMVMDFSEVKRIVREQIVDPWDHAFLVYKNDTMVVDFLRSLENHKTVILDSQP
                     TAENLANVAFLILDEAFQKNYGDRLRLERVRLYETPNCWADATRAHHGI"
     gene            22650..>22980
                     /locus_tag="PKZ49_01990"
     CDS             22650..>22980
                     /locus_tag="PKZ49_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HND35362.1"
                     /translation="MFIDSHCHLDFPDLAANLPELLDNMVQNQVSHALCVSVNMEDFP
                     RVRALAENYDHLYASVGVHPDYENITEPTVAQLVELAQHPKVIAIGETGLDYFRLKGD
                     LEWQRERF"
BASE COUNT         6740 a         5129 c         4696 g         6415 t
ORIGIN      
        1 tcattctttc aatagttgcc attgtttctc ggaggaggct cttctcttca tctagtgcta
       61 tcggtttact cgtcatcgcc tctgcttctt gcgcggttta ctatcaatgg ctaaatcccc
      121 caaagctagc gttctctgag tggcttcata ctgaagtcgc tgcatacggg ttactctgta
      181 ccgctttttg ggtttcttca ggtaggccaa tgccgtctag atttacttca cactctctcg
      241 ctccaatact gttaataacg ggtattgtac ttcattggtg gtctactcca ctcgtaatac
      301 agcagttgat tggtgtttta tgctttgttg gcgccatctg cgtacttagc actaccaaag
      361 gctggtttgc aagtgctctt tcatggtctc ctctccgcaa acttggtcta tggtcgtact
      421 cactttattt atggcagcag cctttttatc tttggagtca cagcgaacaa ggtccatcag
      481 cctggatagc tttgtgcgca gctctgtttt gtggattgat ctcatactat ttcgtagagc
      541 agccagtaag aatctacttg aataccaatt ggaaaacaag aagacgcgac agctagctta
      601 gctgctttgg gcgggtgtat tctagctcaa aggggataac tccaactccc agagcgactc
      661 acaaaacatt tttgcattgc gcagttatcc taaaacgttc tctcagtagg aataaccact
      721 tgatagtttt ctatcgtgaa acttatcata aatgcgctga ttgtctttaa aaatctgtac
      781 atttttactt aaaaaagcga taatttcgta acaatgcaaa aaaatatcac tgctaaaaat
      841 atcctggtta tccatggacc aaatctaaat ttattgggaa taagagaacc tgatatctat
      901 ggcactgtaa cattagaagt aattaatcat aagttacttg aaatggctca agctgccggt
      961 atacaactta atatcttcca aaataatagc gaaggcctat taattgatcg tatccattct
     1021 acgttccagg atggaacaga ggctattatc attaatccgg ctgggttgac acatacgagt
     1081 gtcgcattac gggatgcact tgccgcaacc aacttaccct ttgttgaagt acacctgtct
     1141 aatatatata cccgcgaatc gtttcgccac aaatcttatt tctcagacct tgcagttggg
     1201 attattagtg gcttaggtca taaaggttat gagtttgcac tgcaattttt tattaaatca
     1261 taactatttc ttaatacacg tttagatagg atagatatca ctgaaatgtt tttaggcttg
     1321 aggtcaaatt taaacttctt taattgtcat gaaaaaaact gaaaatagcg aaaaaatacg
     1381 cgacaaggcg tttactttag gaggagtttt atggatttaa gaaaactgaa gaagctattt
     1441 gaattagttg atgaacataa tattactgaa cttgagataa cagaaggtga agaaaaagtt
     1501 cgtatcagca aaaattcgaa ccaaattatc caaggctata ctataccacc ccagcaatct
     1561 catttttctt atagcggtgt ggcagaagcc tctcagaaac aaactgttaa tgaatctgtt
     1621 gatagcgatg acaaaccaaa aacagaatta ccggatggtc atattgtgac ctcccctatg
     1681 gtcggtacgt tctatcgttc ctcagcacca ggtgcaaaac ctttcgtgga aaccggtcat
     1741 agtgtcaaag taggcgatac actctgcatt attgaggcaa tgaagctatt gaatgagatc
     1801 gatgcggata aagatggcat catcaaatcg atattagttg agaatggcca accggttgaa
     1861 tacggccagc cgttattcgt cattggataa gcaagtactg atataaatta tcctttctcc
     1921 cttcagattt tattttttta tatgagtcaa catgtttgaa aaaattctca tagcgaatcg
     1981 gggtgagatt gcgctacgta ttcaacgtgc atgccgggct atgggtatca aaaccgtagc
     2041 agttcattct gaagcagacg cagatgcgaa atatgtcaaa ctgtcagatg agtcggtatg
     2101 catcggccca gcgccatcag cgcaaagtta cctcaatatc cctgccatca tcagcgctgc
     2161 agaggtaacc gatgccgaag ccattcatcc aggttatggt tttctctcag aaaatgcaga
     2221 ctttgcacaa cgtgtcgaag aaagtgggtt tgcattcatt gggcctcgcc cagaaaccat
     2281 tcgactcatg ggtgataaag tctctgcaaa aaatgccatg cgacaagcag gcgtaccttg
     2341 cgttcccgga tcggatggcg ctttacctga atcgctcgat gaaatcaaaa aaatagcaaa
     2401 atcaattggc tatcctatca ttatcaaagc agccggtggc ggtggtgggc gaggcatgcg
     2461 cgtggttcat accgaggcag ccttatcaaa tgcgctgatt accacacgca atgaagcgca
     2521 ggctgctttt agtaatccaa cgatctatgc agagaagtac cttgagaatc cgcgtcatat
     2581 tgaatttcag gtgttggtcg attcgtatgg taacgctgtc cacttaggcg aaagagattg
     2641 ctctatgcag cgccgtcatc aaaaaattct tgaagaagcg ccggctgtaa aaatttcttc
     2701 gagattacgc gataaaattg gagaacgctg tactgaagcc tgtcgtcgca ttaattatcg
     2761 cggtgttggt acatttgagt ttttgtttga gaacaatgaa ttttatttca ttgagatgaa
     2821 tactcgtttg caagttgaac atccggtgac tgaaacagtg acagggatcg atttagttcg
     2881 tgcgcagatc aaaattgcag caggcgaaaa attggagtta aagcagaaag atattatttt
     2941 ccaaggccat gcgattgaat gtcgtatcaa tgctgaagac ccctacaaac taacgccttc
     3001 agcaggacgt attttgcaat accattcgcc gggagggcct ggtattcgtg ttgatacaca
     3061 tgtgtatcac aattatatgg ttccacctta ctacgattcg atgattggta aaatcattgc
     3121 ctatggggat gatcgtgaac aagctatcgc acgaatgcgt atcgcgctct cagaaatggt
     3181 cattcatggt ataaaaacta atataccgct tcatctcgat ttattatccg acagtcattt
     3241 tctagatggg ggtacttcga ttcattatct agaacacaag ctcgctctgc ataataaatc
     3301 ggataaaggt gatcgttaac ttttcctttg ctcaatcatg tattggaact cgctaacact
     3361 cgatgttgat gcagctcaag tagaattagt gagtgaaaaa tgctttgcgt tgggcgcatt
     3421 atcaattgat atccgtgatg cagcagccga tactgccgac gaaaaacctt tgtttggcga
     3481 acccgatgtt atccatgatg aaatctggga ccatactttt gtttccgctt tatttgaaca
     3541 ccatgtcgat attgcaatca ttttgcaaga gatcaaacaa acattaaatc ttccttatcc
     3601 acccaaatat gctttagctc aagtacatga gcaggattgg gtaaaattaa cacaatcaca
     3661 gttcgacccc attaaaatct ctcaacgatt gtggattgtt ccaacttggc acaccccagt
     3721 cgatacgaat gcgattaact tgattctcga tcctggtcgt gcgtttggta ccggtagtca
     3781 cccaacgacg caattatgcc tttcctggtt agaccaacaa tttcaacccg gtgatgccgt
     3841 gcttgattat ggatgtggct caggcatatt agcaattgct gcattaaaat tgggtgcaag
     3901 ccgggtcatt ggagttgata tcgatgcgaa tgcaattcaa gcgagtcaag aaaatgcgat
     3961 gattaatcaa tgcaatccgc agtctatttt attctcgaca tcgctcaatc tgccaattgc
     4021 taattttttt gagaagcgat atgtcgacaa agtcgtcgcc aatatcttag ccaatccact
     4081 catcctatta gcgccgttat tagccaatgc cgttcgggaa ggaggagaaa ttgcactttc
     4141 tgggatattg agagaacaag ctgacgaagt ttgccaatgc tatcggcaat ggtttgatat
     4201 cgatattgtc gatcaaaaag atgactgggt actgctagta ggaacgaaat accttgatta
     4261 aaagttgcta ggctatggtt ctcgtaacga agtgccctgt ttgccaatca gtttatcgcg
     4321 ttacttttcc agaattacat ttgcacgatg gaaaagtaag gtgtggacaa tgtgaacaag
     4381 cttttaatgc ctttagggcg ctgatttcaa ttccagaatc gcttatcaga atggaggaca
     4441 gccaactcgc gcttcccgtt catgaagaag ttgaacttcc cgctattaat aaacaccctg
     4501 cgccatcagg cctgcaatcc agcgagcaag cggagttaac gagcgcatct atagccgatc
     4561 aagctgatca aaatggggta gctgaagtac acacccccaa cttgatttgg aaacaagttg
     4621 ctgcaagctt agctttaatt atcctgttgt gcgtacaact catttacttt taccgtacag
     4681 aactcgcaat cgcgattcca agttccagag cgattctaga aaaaagttgc gatctattgc
     4741 aatgtgaagt gcacttacca aaatacagcg ccttattgag tcttgaatcc tctgaattac
     4801 gtgtcaatcc taaccatcaa gctgaaattg tgacattact tgcaacgatc cgtaatcacg
     4861 cgccctttca gcaagcatgg cctgaattac gattaacact aaccgatctt gatgatcaac
     4921 ccattgcaag ccgaattttg ttggcaagtg actatctaaa cgataataaa ctgccacaat
     4981 tcttcgctcc ggatagcgaa atagaaatca aaatctattt tgataatagt gagttgaaag
     5041 ccatggggta tcgtttggag ttaatctatc tttaaacgat ataggtggtt caagttgatg
     5101 atgcacaaac acgattgcat caattttcaa gttgcgtgat gattgtaaac tgtcctgaaa
     5161 gcactttatg gataggacat ttattagcaa ctcgtaacaa atcatcgcgt tgcgcctcgg
     5221 taagctgccc ttttaattga atattgcgct gtaattggta taaacctgct tcttcagtgt
     5281 gcgtaatcga tacgagaaca cgctctaatg gtatcgcctt acgtttcgca tagatttgaa
     5341 tggtaagaac agtgcaagcg ccaagcgcag agtcgagcat ctgatgcgga tcaggatatt
     5401 gaccagaccc accagactct agcccgatat ccgagatcca agtttttcca ttttcttgta
     5461 tttggcaagc cgttttgttg ctagctgctt cccaactgat ttcaatggcc atggcactcc
     5521 ccgatttgaa agttataatt cagtatgaat ttagcctaga ttttttacgt tagaatctgc
     5581 tcgctgctta atctgatgag ttccatattt cacatacacg gtttaactga tagcagtgaa
     5641 attagggcgt tgcgattagc ttactctctg cgcttcttaa attgctttga atcacttttg
     5701 tgttcgggtg atcttcccca agtaattttt tagctgtaac cagcgcctgt ttgtaataat
     5761 cgatggcatt ttggtaatgt cccgatgaat accaagccca gccaagattg ttgcgatatg
     5821 tagtaacttg tggatgattc tcaccatgcg attgcaacac aatggaaagc gcctgatcaa
     5881 aatagtagat ggccttttga tgctcaccca aggattccca agcgttcccc aaattatttt
     5941 tgtcgcgcgc aatgttgggg tgattctgac catatgcctt taaatcaatc gccaatgctt
     6001 gctcataata actgatcgct ttgtgcaatt ccccaagcga ttcccacgca ttgccgagat
     6061 tattacgggt caccgcaatg gaaggatgat tttcatcatg caatgcaagc ccagtctcaa
     6121 gcgcttgctc gaaatattcg atggcttttc gatatttgcc tagtgagtcc catgcgttac
     6181 ccaaattatt caggtaagta acgatcaatg gatgatgctt cccataaatc gcaacactgg
     6241 tttcgagtgc ttgctcaaaa tactcaatgg ctttgtgata ctcgccgagc gattcccagg
     6301 caattcctaa atcattacga tctctggcaa tactagggtg attggtttta taggttttaa
     6361 ggtcgctatc aagagccgct tcgaaatact gaatggcatc atggtaggct tcaatagatt
     6421 cccaagcatg gtcgatttga ttacggcgtt tggcaatact cggatgatct tctccataat
     6481 gactgagatc agcttccagt gattgttcat aatatcgaat cgcgcgagga tagtcaccta
     6541 atgagtccca agccattcca atgttattct gaatggcagc gacatcaggg tgattttctc
     6601 catgcaattg aatcaaactt tccagtgctt gctcaaaaaa cttgatcgct ttggcgtatt
     6661 caccgagtgc atcccatgcc atacccagat cattctttac tgcagcaatc tcaggatgat
     6721 tttcagagtg tagcgtgcgc agaattgcga gtgcttgctc atggtaacta atggctttct
     6781 tgtagtgcgc taatttatcg ctaaaaaatc ccgccgcgct gaggtataac gcattattag
     6841 gaatcaagcg gatcgccttt tctgcatgtt caagtcctgc cgtatagcga actgaatctt
     6901 cagctaattt gctcaattgg taagctgctt cagccgcaag gggtggattg tgctcgttat
     6961 ttttttcgat ttgacggaac aattgaatcg cctgctgatc attcgcctga cgtaaagcca
     7021 taatagcctg ttctagcatt tgaataggat aagcttgatg aagtacttct aattgtttta
     7081 ggcggtcatt gatacttgtt tttagcagta agaaactgtt atcaatatcc tgtaaaagca
     7141 gctggatatc ctgcaattca acttcgagaa tgcgcctatg tttagggtca gtgtgcttaa
     7201 gcacctcgag aatttctgct tcacgctgct gtaatttttg ttgataaaac tgctttgtga
     7261 gatcttgctg aagatgagtt gagctttcag caatgcgttc ttccgattta tttgaaaaaa
     7321 gcagcaggcc aaataccgta atgagcccga taataaagaa aaataatgcg atttgctcat
     7381 tcttcgataa cgaatagtca tatttcagca taatttccgc tcgcacctta aatcagatta
     7441 gatttcacaa cgaattaaca tatcataaca agacggagaa cgcccgtgtt tgtaaaatcg
     7501 aattagcttt gctaaagaag aacgaggttt tccaaaataa acccactcga agtttataaa
     7561 ggcaacgcga tgtgaagcag gaaagaatga agaataagaa tcaaaccagt tatttaccgg
     7621 tgcggtgacg gaatttgacg atcgccattt gactcatcaa aaataaaata agcttgtcag
     7681 atccgataat ttagatcatg acaagctttt atactaatac ctcatttctg ttaaagctat
     7741 tatcactaca taacatagtt agcatttcgc agaactttca tcgttattat tatatatttt
     7801 taataagcaa ttcacatgcc aattaatttt tacacctgtt attcattgat ttttatagat
     7861 aataaaagca actccaatcc tttcgatcag aattaaatga taactgtaaa ataatccgac
     7921 gctttttctt agtgagattt tgttagatat cataagcacg aaaaaatatc acactcactg
     7981 gaaaattgtt cgagggcaat tcgagtgtaa aaattcctga cataaaaaat atttaaaaaa
     8041 ataaatgtag tttaatgact tcactcgggc tttagctcaa ccataacccg accaaacaaa
     8101 ctgcaaaatg ataaactagc agacgaatac acaattctca atgcaaacaa tttatctgta
     8161 agagacccta tgattattgg agtacctaaa gaaacaaaag accatgagtt tcgagttggg
     8221 ctgacacctg ggactgttca cgcactcacc acacaaggac accatataaa aatagaaagt
     8281 ggtgcaggat taggtagtgc tttcactgat gatgactatc gattggcagg cgctgaaatc
     8341 gtaacgtacc ctagagatgc ttgggctgcc cagttagtta tcaaagtaaa ggaacccact
     8401 gaatccgaat atgcttatct aaggcaagat ttgattctat ttacctacct gcatttggcc
     8461 tcaaataaac cgctgactga acaactgctt gcgacaggaa caaccggtat agcctatgaa
     8521 acggtacaat tagcatcagg tgaattaccc ctactcacgc cgatgagtga agttgctgga
     8581 agaatggctg ctcaaattgg agcgctctat ttacaaaaaa cgttgggtgg aagaggaata
     8641 ttgatggggg gtgtccctgg cgttgctccg gctaatgtcg tcatccttgg tgcgggcatt
     8701 gtcggtgtta atgctgctag agtgactgtg ggaatgggtg cgcatgttac gatcttagat
     8761 atcaatcata gtcgtttgaa atacctcgat gatttgtttc gtggacaact ctttacccgc
     8821 atgaatgatt cattcaacct caatgaactc gtttatcaag ccgatctcgt tatcggtgcc
     8881 gtattgattc caggtgggcg ggcgccttgg ctcatcactc gagaaatgct aacaaaaatg
     8941 cgcaaaggcg ctgttatcgt ggatgttgcc gtagatcagg gggggtgcgt cgaaacgacc
     9001 catccaacca cacatagtaa ccccgtttat gaagtcgatg gtattctgca ttattgtgtt
     9061 accaacatgc cgggcgctgt accccgtacc agtacgcttg cattaaatag tcagactgcc
     9121 acttatatta agctactcgc tgatagcgga ttagatgcag taagaaacaa taacgcatta
     9181 cagaaaggtc tcaatactta tcagggattg atcactcaca cagcggttgc tgaagcattt
     9241 ggactacaat ctaccgcgcc gttacaggcg cttgcttgac aaatgacact tagcagagca
     9301 acccgtgacc ataaccgctt ttgctaactg atcattattc ttaatcatag tataatttac
     9361 cccattctga atgacgcttt atccctaacc ttattatttt tcttgttaat tatcgtataa
     9421 gaatgacctc attactttct gctaaacaac tgctaccgat tcttctgctc tgcattagct
     9481 ttttctttat ggctagccca ctccatgcgc aagaagtcgt cgtttattca gcccgaatgg
     9541 agcagttaat caaacccatg tttgatgcat tcacaactga aactggcatc aaagtcaaat
     9601 ttactaccga taaagaaggc gctttattag caagactcca agcagaaggc aaaagaaccc
     9661 ccgctgacat attgatcacc tcagatgcag ggaatctatg ggaagctgct agagctggat
     9721 tactaaaatc aatttcttcg ccgattttag aagccaatat cccagtacaa ctgagagatc
     9781 cacaaggcca atggtatggc ttatcgatac gagcacgcac cctcgtctat aacaaggaaa
     9841 aagttgatcc ttctgaactt tcaacctatg aagacttagc aaaccctaaa tggaaggggc
     9901 gcctgtgttt gcgctcttcc aaaaaagtgt ataaccaatc gctcgttgca atgatgattg
     9961 ccgagcatgg tgaagcaaag accgagcaaa tcgttaaggg ttgggtagca aatcttgcca
    10021 ccgaaccatt atcagacgat accaaagcaa tggaatatgt tgcagcaggc cgctgtgatg
    10081 tcacattagt caatagctat tattatggtc gattgatgga aacgaaccca aatttaccgc
    10141 tggttattta ttggcccaac caagataaca atggtaccca catcaacatt tcaggtgctg
    10201 gcgtaacaca acatagtaaa catgaagctg cagcaatcaa gttacttgaa ttcctgtctt
    10261 ctgagaaagc ccaaaattta tttgcggatg ttaactttga atttcctgcc aatccccaaa
    10321 ttaagccaag cccgattgtt acgagttggg gtgattttaa gaaaagtccc atgagtatga
    10381 ttaaagctgg ggaattacaa gccactgcgg ttaaattaat ggatcgtgcc ggctaccgct
    10441 aattgggtca atccatgtga atgacgaaca gccggataat ttgggagcaa aactttcagc
    10501 cagccatcga catccctctc ctcaactaaa aaataacatc aatttctggc gtttagctcc
    10561 tttctgcgtt gccagtatcg ttttaatacc gatcattgtt gttatttcct cgatttttac
    10621 accagcagat gatatatggc atcatcttgc tgaaacaacc ctaccctcag tcctgttgaa
    10681 tacattctgg ttatgtttag gcgtcgtctt gggagcgatt atcctgggca ctagcctagc
    10741 ttggttaact gctatttatg attttcctgg aagaaaattc ttttcttggg cgttattgct
    10801 accgatggcg attcctgctt atgtcactgc atttgtggta ctaggtctat tcgactacac
    10861 agggccgatt caaacctatt taagaagttg gactggtgcg gaatctaatt ggattcctga
    10921 tttttacggt agaagtgggg ctattctcgt aatggtcctt gcgttttatc cctatattta
    10981 ccttttagcg cgtaatgcat ttataacgca gggaaaacga ctgattgaag cagctcaatc
    11041 gctcggcttg agtcgcaaac aaggattctt taaagttgca cttccgatgg cgagaccttg
    11101 gattataggg ggcgccatgt taactttgat ggaaacactt gccgattttg gaacggtctc
    11161 ggtattcaat tacgatacct tcacaaccgc catctataaa gcatggttta gtttgttctc
    11221 actgtctgca gcttcccagc ttgcatcatt attgatcatt cttgtcttta tcatcattat
    11281 cattgagcag caacttcgtg cccggatgcg ctttgccgaa tcaaaatcaa gcagaacgac
    11341 ctttcgtatc ccactcttag gttggaagcg ccttgcagct tgtagttacg ctttctcgat
    11401 cctttttatc gcttttattt tgccttgctc tcaattactt atttgggcaa tcgatgaaat
    11461 taataaaaat atcgactcac ggtatattga atttctcatc cactcattaa cgttgtccac
    11521 aatcgctaca ttgatcatcg gctcggtcgc tattttgctg gtttacgcta ctcgccttta
    11581 ttcagataaa gccacgcaca tcgcggttcg tattgctacg cttggctatg cgttacctgg
    11641 cgcagtactc gcagtcggta tttttatatc gctagcttgg atcgatacac aactcaatca
    11701 aatcattctg atattatttg agatcgaagc tggttttctg attcaaggaa ccattatagc
    11761 catgcttatc gcttacttgg ttcgtttctt ggcggtaggt cattatccaa ttgatagcgc
    11821 gatgcagcgt gttactcgca gtatcgatga agctgcgcgc ggattcgggc taaccggttg
    11881 gtcattactt tctcgtatcc acttgccgat tctcagctcg ggtattttta cggctgccac
    11941 ccttgttttt gtcgatgtga tgaaagaaat gcccattacg ctgatgactc ggccttttgg
    12001 ctgggacacg ctagccatcc gaattttcac tttaacctca gaaggcgcat ggaatcaggc
    12061 tgcattgcct gccattgcac tcgtattagc cggtttaatt cccattatct tattgatgcg
    12121 acaaactgaa cagtaaacat cgtggaatca ttattaaaat taacccaaat acaccagtcc
    12181 tatcatcagc aagaaatcat caaacacctc tcgctcgaat tgaaaaaagg tgaaatcggt
    12241 tgtttattag gaccaagcgg ttgtgggaaa acgacggtat tgcgatgtat cgctgggttt
    12301 gagtccatct catctggtga aatcacgatc aatgggaaaa tcgtctgcaa tgctgatttc
    12361 aatctaccac cggagcaacg aagaattggg atggttttcc aagactatgc actatttcca
    12421 caccttagcg tttcagccaa tattggcttt ggtttacaac aatacacgaa agctgaacgt
    12481 actcatcgaa tcaatgaatt gataactacc gttggactca ctgaggcttc cgaccgctat
    12541 cctcacgaac tctccggagg acaacaacaa cgcgttgcat tagcacgcgc acttgcccct
    12601 cgtcctgacc tgctactgct tgacgaacct ttctcgaatt tggatattag tcttcgtgaa
    12661 cgcttgagtt tagaagtgag agatatttta aaagaccaac atacgacagc catattggtc
    12721 acccacgacc aaactgaagc tttctcggtt gcggatgtta tcggcgtcat gcatcaaggc
    12781 gaaattttgc agtgggatac ggcttacaat ctttatcatc atcctacaaa tcgctttgta
    12841 gcacaattta tcggacaagg tacttttgtt ccaggccatt tgatttcacc tgaaaaaatt
    12901 caaatagcgt taggtatcgt ggaaaatccc gattattcag attactttga gcaatctgaa
    12961 cataccacca acgaagcagt cgatgtttta ctccgacccg atgatattgt ttacaacgaa
    13021 gctagtgctt tacaagcaac cgtcgtcaat aaggcattcc gcggtgcaaa tatcctctac
    13081 acactcaagc tatctagcgg acaaactctc ctgtcgttaa tcccgagtca ccacaatcat
    13141 tcgattggtg aaagtatcgg gatccagttg gcagcggatc acgttgtcgt gtttagtaga
    13201 gagaaaatgt aaccacccat cagtaacttt ttagcaaata caggattaat cctttgagaa
    13261 aaaccaattt ttttgccaag cactaaatac caaatccgga taggttgctg aacccaggct
    13321 gccattatat cgacctagtg ctctgaaata atcacctttt tctaaatcaa gataatgccg
    13381 gaaaatggta cacccatagc gcaaattaac gcgcaaatga aacaaattat gttctttgct
    13441 cccaatggta tcaatccaaa atggcattat ttgcatatac cctcttgcac cagccgatga
    13501 aattgcgtat ttgcgaaatc tactctcgac atgaatgata ctcagcacaa gctgagaatc
    13561 aagaccggct cgcatcgcct cgtaatgaac tgtcgttaag aaaccttcac gctcattctg
    13621 atcaggcata tatttttcca tacgcgctga catgaccgca agccatcttc tactctccgc
    13681 atcaggcaaa gtcacgaatt gagcaatcgg ctgatcgcta atcgctttct gaagcacagt
    13741 tcgagtactt gctgataatg gctcataaag ttgagcacct gcaaaaatcc agcaaggctt
    13801 cagtaataaa atgattaata ctaggttccg catacctttg cgcgaagaaa atgatcggct
    13861 tcagcaattg gaatcatttg agccgcttga tcacgacgcc ctcgatactc tgctgcacct
    13921 tctttgaggc cacgttctcc aatcacgata cgatgcggga taccaattaa ctccatgtcg
    13981 gcaaacatga cgccaggacg ttcatcccga tcatccaaaa acacttccac acccgcttga
    14041 gataacgtct gatacagttg atcagcggcc tgttgtactg attgattttt atggtatcca
    14101 atcggcacga tagcaacttc aaaaggtgca attgcggtag gaaatataat ccctgcagca
    14161 tcatggtttt gttcaatcgc agctgccaca acgcgcgaaa cgccaatacc gtaacatccc
    14221 atttccatta ccttgatttg ccctgcatca tccagaaaag ttgctttcat cttttccgaa
    14281 tatttagtcc gcaactggaa aatatggccg acttcgatac cgcggcatat ctctaactgc
    14341 cctttgccat cgggagaaag atctcctgct acgacattgc gaagatcaaa acagcgatca
    14401 ggcactttca aatcctggcc gaaattgact tgggtaaaat gaaaattttc ttcatttgcg
    14461 ccacacacaa aattactcat ctccaatacc gatcgatcag cgatgaggta cttttccata
    14521 ccaatcggtc cgatataacc aggcttacag cccgtttctg taatgatcct ttcttcactt
    14581 gcgaattcaa aattagtcag taatggaatt tttctgactt ttgtttcatt gagctgatgg
    14641 tcccctcgca gcaacaataa aaaaaattgc tcatccgcag taattgcaag tgcttttacc
    14701 gttttctgaa tcggaatccc tagaaattcc gcaacctcct ggcaagtttt gataccaggt
    14761 gtggaaattt tattttttgc tccagtaggc gcttcagatg gcattgaatt tccaggcaag
    14821 gatgtggcta actccacatt ggctgcataa tccgaatggg gacaaaatgc aatcgcatct
    14881 tcccccgagt ctgcaagcac atgaaattcg tgtgaactgc tgccccccat tgctcccgta
    14941 tcggcagcca ctgctcgaaa atctaacccg attcgagtga atatcctcga ataggtttca
    15001 tacatgattt ggtacgtttt ttctaagctc gcttgatcta catgaaacga ataggcatcc
    15061 ttcatgataa attcacgcgc acgcatcacc ccaaatcgtg gacgaatctc atcacgaaat
    15121 ttggtttgaa tttggtaaaa gttgatgggt aattgacggt agcttttaat ttcacgacga
    15181 acaatatcgg taatcacttc ctcatgcgtc ggtccaaaac aatagtcatt ctcatgccta
    15241 tctttgattt tcagcatctg tggtccaaat tgttcccatc gtgctgtttc ttgccacagc
    15301 tctgcaggtt gtacagccgg cattaatagc tcttgtgccc cacttcgatt catctcttct
    15361 cgtactatct gttcaacttt ccttaaaact ctcagcccca atggcatcca actataaagc
    15421 ccacttccca aacgtttgat caaaccagcc ctaaccatga gtttatggct aattaattct
    15481 gcttctgctg gcgcttcttt aagtgtcgaa agaaagaaat tagaaacacg cataaaattg
    15541 attccactta tgattatttt atttaaaacg tgattctacc ttgaaaacca atcatcaata
    15601 cgttatagac ttgccagcag ctcatcttag cttgataaaa attgcgcaaa ggttagagca
    15661 aaaggaaaaa gtcccagctt tttgttttat ggtggctttt catcttatcc aaagtatgaa
    15721 aaaatgtccg ctaattaacg aattgaatga cggatgaatc aaatgattga tcggaacgga
    15781 tatcgtccta atgttggtat cattctgcta aattcaaaaa atgaagtttt ttggggaaag
    15841 cgagtcaaac aaaattcttg gcaatttcct caagggggaa tcaaaacagg agaaacccca
    15901 acacaggcga tgtatagaga actaacggaa gaaattggat tacaaccgaa tcatgtagaa
    15961 attcttggaa gaacgcgtga atggctacga tataacgtac cggaatgttg gatccgacgt
    16021 gagtgtcgca aaagctacaa aggacaaaaa caaatctggt ttttgttgcg attattggga
    16081 cgcgatacca atgtttcact gagaaaaagc ccacaccctg agtttgacgc atggcgctgg
    16141 aaccagtatt gggtagagct agactctgtc gtagaattca agcgacaagt ttatcgtcaa
    16201 gcgttgctgg aattgtctag actcttggga cgggatatcg ggtcggctta tgattatggt
    16261 tatatcgaaa acgcgcaatc caacacgaat aaagtattat ccaattctac agtaacccta
    16321 aaagattaat ttcatcgcga taggatatga tgagataaaa tcttactact aaattttaat
    16381 atgactttat tagatattat tccaccgaat aatattttgg cgtaatcggc tgcgccatta
    16441 cagccgtgct tttgaggaga gattctaaat gatgatgcgc aagctaactg tttcgctatt
    16501 atcaatcggt gtaatcatgg catcgatgca tgtcactgct gctaacgaac cgattcaacc
    16561 tatcaaacca gtaaagccag aaaacgcaga attggttgaa ttaggaaaaa tgctattttt
    16621 tgatcccaga ttatccaaat ctggtttcat ttcttgcaat tcttgccata atttgagcat
    16681 gggtggaacc gataacatcc aaacatcaat tggccataaa tggcagcaag gtccaattaa
    16741 cgcacctacc gtattgaacg caagcatgaa cttagctcaa ttttgggatg gtcgtgcaaa
    16801 agatctaaaa gaacaagcgg gtggcccgat tgcaaatcca ggcgaaatgg cttcaactca
    16861 tgatatcgca gtgaaagtaa tcaactctat gccgcaatat caagcgcagt ttaaaaaagc
    16921 gtttggctca gatggcgtag atattgatcg tgttaccacg gcaatcgctg cttttgaaga
    16981 aaccctagtt actccaggtt cacgttttga taaatggtta gaaggtgata aaaacgcctt
    17041 atccaaagaa gaacaagatg gttataacgt gttcaaaact agtggctgtg caaaatgcca
    17101 taatggtcct gccgtaggcg gaaactcttt tgaaaaaatg ggcgttcata agccttatga
    17161 aaccaaaagc actgctgaag gccgtaaagg agtaaccggt aaagaagaag acaatttaca
    17221 tttcaaagta cctactttgc gcaatgtaga actaacctat ccgtacttcc acgatggtgg
    17281 cgcagctacg ttagaagaag cggttgatac catggctaaa atacaattga acaaagaatt
    17341 gagcaaagca gaaattgcta atgtcgttgc tttcttaaaa acattgacag gtaagcaacc
    17401 tgacttcaag ttaccaattt taccgccttc taacaataac acaccaagac ctgagccttt
    17461 tgacaagtaa tagcttgttc gactgagtct ttaaaaacaa ctgtcgtcca ttaataaagg
    17521 ccacacaata atttaatttt gtgtggcctt tttatgtgct cctttaataa ccattaatgc
    17581 acatgatcat ccggcggtgg ttttattttt tcttcctgat tcaatatcgt gagttgcttg
    17641 gttagaacgc ctgtaatcaa ccaaccgaag ccatcaaact gactaaccca tcgagcgcgt
    17701 agatacccaa aacgatcaat catgtattca atatgctctg gagtcatccc tttcctgagt
    17761 aaatctggaa ctgccatcgt tctgcgaaat aataaataac tatctctaat ctcagaccaa
    17821 ccttcagtta tgactgggaa tgggacgatg cgcgttactg ctgccatttc ttcctcagtg
    17881 agcgccctat tcgggattaa tagtatttca gtgtgaaaat catttttcaa catctcgtag
    17941 atatcactta actgaaataa tcgctgttgc gattgcggcc atgaaaacaa caccagtaat
    18001 acattacttt tttcacggaa gtcttttaag ctcccaccag agccatcatg cgcaaaataa
    18061 gaaaaaacag gcggtgctag aaaaggctga ttgggtgtca ctaagctacc caataacctt
    18121 cccatataac ctctggaaag tgcatgaaga taattgacaa catcccaacg gttctcatcc
    18181 gttaagacgg ctgaaaaacc tggcatactg gtgccctcaa ccccatacga taaccaatga
    18241 taaaaattac cgacagtatg tctagctgta tgcgttttgg taagtaaatc agtgggatcc
    18301 ggtggaaact cacccgtttc gggatcagca acaaaaagct caggcgctgt acccgtgccc
    18361 tttccctgag gaccatgaca actgacgcag tgttcagcaa atagtgtagc gcccttcgat
    18421 attgaaattg cgtcaaatgg aatgggtgtt ttttgatagg tttcgggata ggcttcaatg
    18481 attagcgggg gcaacgcaat ccccagtgaa acaagaccca tgataaccgg aagaatgact
    18541 cgctttgact gattccatcg attcagcgtt cccatggcgg cgatccctat cgccaggatg
    18601 aataataaag tacccgccca gaaaaagttt tgaactccag gttcgtccca agtcgcatca
    18661 atcgagagcc ggaaaggata aggccaatga tcgatgatcg catgtttacc tggaagcgta
    18721 ttggctaata aggtagcgac taataccagg atgagtgcaa gcagaaattc aatcccaacc
    18781 cattttttta actgaccaat cgctacacct gcacgcacat tttcttggat aaatagcggt
    18841 aaccaaacag aacggattcg gtaggcgatc cataaaataa tgcccagtat cgataatttg
    18901 agattaagca accatccgta acgactagat accaatgtat gataatagtc cccaatcata
    18961 cgatcggctg ccatccatcc tgtgaggaat agcagtatca tgacaggcaa cgcaagtact
    19021 gaaaattgtt tcaaaaaatc ggcatgcagc aaaagcgtac cggcttgcga attgttaagc
    19081 gcatcacgaa taatataaat aatcgcaggc aaagtcccaa accaaacccc ggctaataag
    19141 atatgcaatg tatagggagt cactgaagca aaagaaattt cctcagcgcc tgcatgacta
    19201 accaatgatc ccacagtcaa aggtaatgcg gcagttactg caagcagccc gtaatgccat
    19261 cgaacgcgat gcatacgttt aaggcgaaga acgataaaca acaatagcaa agccaaccca
    19321 aaccgcgcta cccagaggtg tcccactcgg gtttttagca caatctccca ccaagcattt
    19381 agatcccagg tattaatcgc atcacccgta gcctgacctg cagtgctagc taaaatacca
    19441 atcaatccca ataaaacgat acctactaac caaggaaata attgctctaa ttttctgacc
    19501 caacccagtt ccggtagttt ttttccaaac caaatcagtg tcaaaaaaac acaactacca
    19561 aataaaacaa gattggcggt tagctgtaac caccttacca acgcagaaaa aaattcgatc
    19621 attgctttac aacattaaaa tcattgacga aactcgggga taaactaaac cttgagcaga
    19681 acgatagata ccagcttgat acataagata aaatgaaaaa acagaaaggc actgcaaaac
    19741 tgccctatac catagcgtat aaccgccttt cattcaagta agccatacta aggcttaaga
    19801 agtcaaaaac caatgattca atttgctacc ccggatgtct cattgcagta aatcgattat
    19861 gattgatcgc aatgataacc actgagacct tctatgagtg accctattat cacttgcccc
    19921 aactgtcatg ctgccattca tttgaccgaa tcgcttgcgg ccccactatt agctgcaacg
    19981 cgtcaacaat ttcagcaaca actcgcgcaa aaagatgaag caatctccca acgtgagcag
    20041 gcaatccgcc aacaagaaca actcctgctc gaagccaaac gcaatgttga tcagcagatt
    20101 accgagcaag ttgctgcgca actcaaggca gaacgcgatc acctcatcgc ggatgaagcc
    20161 aaaaaagcaa aattggcggt tgccgtagag ctcgaaaata atctacgtga attggccaat
    20221 ctcaaggaat tactacaaac ccgcgagcaa aaacttagcg aagcacaagc ggctcaagcc
    20281 gatttgttaa aaaagcaacg cgaactcgat gataccaaac gtgaactcga actcaccatc
    20341 gaaaaacggg tacaacaaaa tctaaccaca attcgcaacc aagcgcgcca agaagcggaa
    20401 gaaagcctca agctgagagt catggaaaaa gaccaaacca tcgctgccat gcatcagaaa
    20461 atcgaggaac ttaaacaaaa agctgaacaa ggctcccaac aattacaagg agaagtacaa
    20521 gaacttgaac tggaagcact gctacgcagc aaatttccac aggactccat cgagcccgtc
    20581 cccaaagggg agttcggtgg cgatatctta cacgcagtca tcagccccag cggtctaccg
    20641 agcggattaa ttttatggga atcgaaacgc actaaaaatt ggagcgaatc ttggttaagc
    20701 aaactgcgcg atgatcagcg caacgctaaa gctgagcttt cggtattgat tagccaggca
    20761 ttacccaaag gcatcgaaac ctttgatatc gtcgatggcg tttgggttgt ccatccccgc
    20821 gccacattgc cagtcgctac tattttgcgg cacatgctat tgcaaatgag tcgcaccaaa
    20881 caagtcagtg aaggccagca aaccaaaacc gagctgatgt atcaatacct gactggccca
    20941 cgtttccgtc aacgcgtaga agcgattgtg gaagcattca ccaccatgca aaccgatctc
    21001 gataaagagc gtaaagcaat tttaaaacaa tgggcaaaac gtgaagaaca gattacacat
    21061 gtcatggaag caactgtggg cttatacggt gatttacagg gcattgcggg aaaatcactg
    21121 caagagattg aaggattgga gctggaatat ttagagatca aggattagga catcaaaaaa
    21181 cctactcact ctgatctgat cagccatcaa ttctatagca cgctattcat acaatgtgaa
    21241 ttagtttgat cagcaattat ttttaactgc agttttacaa aaaagataaa ccctacggat
    21301 acgccttaac ttcaataccc agcgccttaa ttctatctgc taattgggct agccactgat
    21361 cggatacttt cgacaaccga tccgcttccg cttgcatcgg ggggcgcgcc aacccataaa
    21421 gcaatacgcc ttgtaacggg atttgagcat cgcgggcttg tgctataaac tccaagtaag
    21481 cgtcaacctc actttcacag ggcggttgat tgtcgatttg gagcatgcac gtttggatcc
    21541 aggtggggca cagcgttgtg gctattttca aatgacgctt aatcgtacca ttactgatgc
    21601 ttgctccatt aatcaattgt ctgcggtctg gaatggcgct atcgaattta aaccaaactt
    21661 cgccattgag cgtggccatc cgcgccaatc cccgctgtac cgcaacccga ttgattaaac
    21721 tcccattggt gatcaacacc agcttgatcg attcagctaa ggcaaaatcg tgtttcacac
    21781 gtgcgatgac ttcgataacc tcgcaaaact ctttggcgct ggtgggttca ccgttacccg
    21841 ataacgcaat atcgttaact cgctgacatt cgggtggcac ttgctgggcc atgaaatctc
    21901 cttccagcaa ttcgcgcaga aagatcctca attcctgttc tagcaacggc aaatcaattt
    21961 tcggtgcaga tccacgcgtt aggttcggca cttgacaata gatacagcgc caattacagg
    22021 cgttattggg gttgagattg atgccgatcg ataccccacc cgagcgacgc gaaattacag
    22081 ggtaaacata gaccatgccg acaatatcgc ggcgatgatc ttgcgttgtt aagattttcg
    22141 tttgattggc aatgatataa tttccctatg ttaattacac gtcgtttaga attcgatgcc
    22201 ggtcatcgga tttccactca taccagccag tgtcggcatt tacatggtca ccgctatgta
    22261 attgaggtca ccttatcggg gaatattatc gctgaggaag gtgtaccaga acaaggcatg
    22321 gtcatggatt tttctgaggt caaacggatc gtacgcgagc aaattgtcga tccgtgggat
    22381 catgcctttc tcgtctataa aaacgatacg atggtcgtcg attttctgcg ttccttagaa
    22441 aaccataaaa cggtcattct ggattcccaa ccgactgctg aaaacttagc taacgttgcg
    22501 tttttgattt tagatgaagc attccagaaa aattatggag atcggctgag attagaacgg
    22561 gtgcgactgt atgaaacccc taattgctgg gcagatgcga cccgagcgca tcacgggatt
    22621 taaccattct ttattggatt tatttgtcta tgttcattga ttcacactgc catctcgatt
    22681 ttcctgatct tgctgcaaac ttgcccgaat tactcgataa catggtacaa aaccaggtaa
    22741 gccatgcgct ctgtgttagc gttaatatgg aagattttcc tcgagtccgc gcgcttgctg
    22801 aaaactacga ccacctctat gcttcagttg gagttcaccc cgattatgaa aacatcactg
    22861 aaccaaccgt ggctcagctc gttgagctcg ctcaacatcc taaagtgatt gccataggtg
    22921 aaacagggtt agattatttt cgattaaaag gagatctgga atggcaacgc gagcgcttcc
//
LOCUS       DAOQKQ010000016        22121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_1005_length_22121_cov_5.642708, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000016 DAOQKQ010000000
VERSION     DAOQKQ010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 22121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22121
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1005_length_22121_cov_5.642708"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..1428
                     /gene="clpX"
                     /locus_tag="PKZ49_01995"
     CDS             142..1428
                     /gene="clpX"
                     /locus_tag="PKZ49_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HND35363.1"
                     /translation="MSEKMNSDKLLYCSFCGKSQHEVRKLIAGPSVFICDECIDLCND
                     IIREEMQTTETAKFVKSNLPVPKEICSILDQYVIGQESAKKILSVAVYNHYKRLRTIS
                     KTVDADDIELAKSNILLIGPTGSGKTLLAQTLARLLDVPFIIADATTLTEAGYVGEDV
                     ENIIQKLLQKCNYDVEKAQQGIIYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIE
                     GTTALVPPQGGRKHPNQEFIQIDTTNILFICGGAFDGIDKIIQARSEKGGIGFGAEVI
                     SRKNQKEINEVLKSIEPEDLIKYGLIPEFVGRLPVVATLDELNEVALIQILTEPKNAL
                     VKQYWKLFNMEGGVELEFRDQALKAIAKKALLRKTGARGLRSILEETLLDIMYDLPSL
                     ENVQKVVIDYGSVNSDIKPILIYSDQSKSAKNPKQA"
     gene            complement(1470..2450)
                     /locus_tag="PKZ49_02000"
     CDS             complement(1470..2450)
                     /locus_tag="PKZ49_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HND35364.1"
                     /translation="MNRNILSLLIAGALTASTYAVAQPKDTINDEHAHHDHSSHSIEQ
                     HKEQSTDQNHDHSHHSEHQHSSDHAISHEHDHATDHNQHTTHSPEHSVSGADDHHHHH
                     MDHDHIMDHEGTMIMGQNFDKLPSSCQSISEEVEITVRAGRKYSAKFPGTAFAFDQQQ
                     WKVKPCSKITWHFVNEDNIRHQFMMHGLPKYLYKLGMFHLEVTGPKTVSGTMIVPGKD
                     ETFLVHCDISHHMEKGMKAQLVVGKGGTDIPSIPGLTPYNLTDTYEAENLPKVGDKVE
                     SSKMVRQITQFTSDNLQTYGVMLIGILLGLLCIPFIKKTLKLRKKTENNN"
     gene            complement(2450..4156)
                     /locus_tag="PKZ49_02005"
     CDS             complement(2450..4156)
                     /locus_tag="PKZ49_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HND35365.1"
                     /translation="MPTYIFIALLAPFIMLSSVTYGASKLPATVKVNPEALTSEPMCD
                     PVIAPSWREAQEIEGVKIEASPVCSPDNPAWIAAAVKGTNNISMDTLMKTGLSPDAII
                     KTDDIDGDGDPDRITIRLEIMELNGKSPDFPGIVPTFDIAPGIQPGAWVFSPKMSGMS
                     TENFESIRANPLLRLPSPVIRVEVGDIVQIVLENTHYFPHSIHLHGVDHPFAHHEHGG
                     QDGVPQTSEIELMPGDRRVYQFQARQPGTMFYHCHVQTHTHLAMGLAGMIIIEENKPN
                     NWLQTLNIGAGHVRHPSVAVREHYDQEYDLHYHAMDKELGQIIQRHNDPRLIARDMNQ
                     RYDITDSTEDYYTLNGLSFPYTLRESLIVVEPDQKIKLRLLNSGGELIAIHTHGHHAT
                     ITHYDGVEHNPVAQIMRDVFDMAPAQRLDLKLETVNDGKHSYGEGDWLIHDHREKGIT
                     TNGMAEGGSMSSIVYKSYLGESNMPKVSHFGIDLNKYFTKEYYERRLPVWQDLDEWSS
                     LGSPGEGSGLEDKTQTSLIYGAIGLLFGLMFYWIFSKRKSITQVMLGTISRINASKGS
                     NQ"
     gene            4426..5313
                     /locus_tag="PKZ49_02010"
     CDS             4426..5313
                     /locus_tag="PKZ49_02010"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HND35366.1"
                     /translation="MLDLDKKQFSKHFRTILEKIEQIPPFPESARLLSDLRQNPDANI
                     DQLSEIIESDPGLTAFILKYARIPIFGYGHRIHSVDQAIRLVLGFNTASSLMMSIASS
                     SCLRMQKHGQLGYIKIWSKTLECAALCRELSLMMSNRSQIDSDLAHLCGLFQNFGYLL
                     FGHFFPKEYSYLNDLVARYPEQSFRTIQLQFFGITHDTIGKYLMNAWNLPEEVIVAIA
                     EQNYPDYSGKHAIYVKLIAVANRLLQSELQNIDGCAYVETTSLIDDLGINENEALKAK
                     ENILGCQNDFAILSQSLAA"
     gene            complement(5329..7452)
                     /gene="pnp"
                     /locus_tag="PKZ49_02015"
     CDS             complement(5329..7452)
                     /gene="pnp"
                     /locus_tag="PKZ49_02015"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HND35367.1"
                     /translation="MKLTKKSFTYGRHEVTLETGEIARQAHGAVIVTMGETVVLVTVV
                     GDKNAKPGQDFFPLTVDYQEKFYSAGRIPGSFFKREGKPSEKETLTSRLIDRPIRPLF
                     PAGFYNEVQILAMVLSSDSEIDADIPAMIGTSAALVISGIPFDGPIGAARVGYFNNEY
                     ILNPTTTELKNTQMNLVVAGTQQAVLMVESEATELSEDVMLGAVVYGHEQMQIVINAI
                     NEFADEAGVVPWEWQPVERDPALIEKVTQAAEADLRNAFKIKQKQARTEAIKAVWQRV
                     YADLGIGTEEGPDEHIVNEIGFDLEAKIVRNQILDGEPRIDGRGTRTVRPITIRHGLL
                     PRTHGSALFTRGETQALVVATLGTARDEQKIDALQGDYSERFMLHYNMPPFATGEIGR
                     VGTPKRREIGHGRLAKRALVSVLPSTEEFGYSLRVVSEITESNGSSSMASVCGGCLAL
                     MDAGVPLKSHVAGIAMGLIKEGNRFAVLTDILGDEDHLGDMDFKVAGTENGITALQMD
                     IKIQGITKEIMQVALLQAKEGRLHILNIMKDALPANRESISTHAPRIIKFKINPEKIR
                     DVIGKGGAVIRALTEETNTTIDITDDGTVMIACVSSEGGEQAKKRIEDITAEVEVGKI
                     YVGTVLKLLDFGAIVSIFPGKDGLLHISQIANERVEDVAAHLKEGQSVRVKVLEADDK
                     GRLRLSMKAAIADDIDNASSAPQPN"
     gene            complement(7587..7856)
                     /gene="rpsO"
                     /locus_tag="PKZ49_02020"
     CDS             complement(7587..7856)
                     /gene="rpsO"
                     /locus_tag="PKZ49_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HND35368.1"
                     /translation="MAVTTEQKAQVISDYQRIKGDTGSPEVQIALLTTRINDLIGHFK
                     AHVKDHHSRRGLLKMVNHRRKLLDYLKRKNSESYLKIIERLSLRK"
     gene            8085..8405
                     /locus_tag="PKZ49_02025"
     CDS             8085..8405
                     /locus_tag="PKZ49_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35369.1"
                     /translation="MKRILLLGMLLIPLITMAEDLSLRQRANYAFEQMKAAERDLTTI
                     TQTIEVKKGRLEYFKQKAIEAEQELANAYKESQEINKRVTSTRNRWEQLSNQLLQGDS
                     ESRD"
     gene            8720..9556
                     /locus_tag="PKZ49_02030"
     CDS             8720..9556
                     /locus_tag="PKZ49_02030"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HND35370.1"
                     /translation="MAKKALCIGINNYPGTHMDLQGCVNDAQDWSNVLQSRGFVISAL
                     LDQQATKAAMMSAMSNLINPAVAGDSLVITFSGHGTYQPDKDGDEVDGLDEALCPYDI
                     QTKGEALTDDEIHTLFKVRKPGVKLVLISDSCHSGTVTRAAAAEPEADAPRPRFMPMG
                     NWMSDKDLPHSRSGGIAPFINIGASTSPFLAAYTRSEDDLLLSGCQEGPNNFSYDAKI
                     SGRYNGAFTYYALKTLKTLSVNATYKDWHDAIRRYLPSASYPQTPQLVGTLTARQMKV
                     FS"
     gene            9635..15130
                     /locus_tag="PKZ49_02035"
     CDS             9635..15130
                     /locus_tag="PKZ49_02035"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HND35371.1"
                     /translation="MSVDEYQYRVIGIEQPTVASPQVKEVKVIKKRSLVPGGTRSANV
                     GDFDIKANEIVHLKLNNGFSLWLRADDLLDEFGKPNISRGSQHTWEIRVDPAKFQANR
                     SARGLAGLAIKVLDFFDVDLQSYTASKLCSLWEDKLLEKKAANLYRCSLEDTYALSEK
                     GELKYEQPLLLFIHGTASNSQKSFGGLWEASSKVGKAARERLKKKYGEAVYAFEHRTL
                     TESPIQNALQLVKQLPNNAKLHLITYSRGGLVGELLCLGDLKSTLPDTETIHNLFAAD
                     RTIALQLGLNPLDESAQKERNKAYDDDREALQTLLKELKQRQIKVERFVRVACPASGT
                     TLASGRLDRWLSALNYLSGDGLFGDTVDFLLAVLKERTDPRTLPGLEAMMPGSALTRL
                     LQDPSLETNADLSIIAGDIEGDSVWSQLKLWVTDWFYGSDHDLVVNTGSMYGGLRRLP
                     QQARFLCDRGPAVHHFNYFNNDKTINWLLQGLERLDGSNGPFQAIEQAKPEQPRWRSA
                     VQRSRSSATPRPLAVVLPGTMGSTLKVGKEEVWLNYWTLAQGGLKKIQMGAIGVQPDD
                     LLDDFYGPLLEFLALTHRVEIVPYDWRLSIREAAKRLIETLQEHLPEAERNYQPVHLI
                     AHSMGGLVVRAMLADGKEGTALWQRIVKLPKSRFLMLGTPNQGSYEAVRWLTGNNATQ
                     AKLSLLDLTQNTDEIANLVRRYPGLLELLPFDPDSKDFTQSNLWADLKAQLSAAWLPP
                     ETLDLVNIRDTWSLLKNPAQSVDPKYTCYVAGCQDATVVDYRLVDCGGWLHANSNCKR
                     FEFLATREGDGTVSWKSGRLPDVPLWYLEDTAHDDLCRQKRAFPGYLDLLMNGDTSHL
                     SKTPPGRARAPVGEPELFALPAYPVIDSIPSEASLRNFGFGSSRPMREMESGLTIPII
                     EVSICHGNLAYAKHPVLVGHYRGDGIFSAEKALDERLECQLSTRIRLGLYPGAIGSHA
                     LFYAKEPNAKPSGAIVIGLGQVGDLTPGLLTLGVQNALLECALQIAQWPAEDTRFSTE
                     AGKRSIDITSMLVGTGAGGMTVRDSIEAILRGAIAANEKLAEAELVDQVAINRLEFLE
                     LYEDIAIAAAEALNLILANNQLSNHVNWPDATVRAGEGGYTRLRYDEMGGWWHRMEIS
                     ETEGREMLRFVSTTDRARAEETLATGQLKLADNFIRQASQDAGANSEAAKTLFEMLLP
                     NRLKELAPQQEGLVLIVDESSARYPWELLENRWSHNGKPPAVSAGLIRQLKTSVFRPS
                     PVHTTKLNALVIGNPNLSGWSSFPDLVGAREEAQKVSELFNGNGFQAIDYIDANADDI
                     LSGLHKDEWRVLHLAGHGVHDFDVSKADSSALDLLDNATQSVYSKKKLISGMVIGKNT
                     FLTPGDIEQMRWVPELVFINCCYLGKTQSVIEKPYGALAANLAAQFIVMGVKAVVSAG
                     WAINDQAGLAFADSFYQKMLLGMSFGDAVRLAREENWNNFKTLNTWGAYQCYGDPDYR
                     LMNKGTDLLAPSTKKKTYYAPAELLTDLKNDTAFIRMQLNDKRKTEAELSDLKQMIDG
                     RLTAIPQDKQKAWLAREDVKIAFGLAYGELRAWDEAIQYLEAALQAGNAECSIKVMEQ
                     LTNFRVRAASQRWSQVDAVTDAKAQHQCRTDIEKAIKELELLCEFFPTSERYSIMGSA
                     YKRLARIQTNKKSLQNALEKMALYYRKAYEKSGSTDYYPFTNMVTANWLIKNLGKPSG
                     NDFDALINDCDRIIEITQEKNSRSPNFWNSVALADCALIKWMLRGELSEASTNEVITL
                     YQRAIQRGASPREIGSVKENFQFLMDLTQSLAQPIHEAINTVFESLP"
     gene            complement(15200..15853)
                     /locus_tag="PKZ49_02040"
     CDS             complement(15200..15853)
                     /locus_tag="PKZ49_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35372.1"
                     /translation="MTDVVEERKKVHAELRTELFKRQLSNSENFDKAILTYSSAGLAL
                     SLGFLKDFIPITQACAAWLLFLSWGCFSLAVVITLSSFISSQQGIKKQLEINDRYYLK
                     MDESALSEKNCCARYTDWLAYAAGFTFVIAIVCSTIFVSINLERIAIMVDKKQASLKE
                     DAPTPTIQQTHKTEQKGAPVPGIQQVPQQTTQSTGFAAATSKSETPASSNTNRNEKP"
     gene            complement(16033..18018)
                     /locus_tag="PKZ49_02045"
     CDS             complement(16033..18018)
                     /locus_tag="PKZ49_02045"
                     /inference="COORDINATES: protein motif:HMM:NF024677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAFs-binding domain-containing protein"
                     /protein_id="HND35373.1"
                     /translation="MQPHAFVAMPFGTKKDSQGNLIDFNRVYRELIQPALRAAGLQPF
                     RADEEHRAGDIRTDMFQELLLADLVIADITIDNPNVWYELGIRHGLRARGVVLICGGE
                     ISTAFDLYTDRKFRYHIKAEGPDPLLVGADIQNLTEMVKATMQSWHGRKTSPVYQLLP
                     HLQEPDWKSLRIGGIQEYWEKYQAWENRIKLAVNAGYLGDVLVLADEAPVAALRAEAW
                     IKAGKALRQHEHFHFALDQLERGLAIEPDNLLGLHEKGICLQRLAQRNESGYSLDRAR
                     THYQSILSQESYQHDAETWALLGRIDKDAWYSAWYQPNQTTQSYRDEAAYQSPLLKNA
                     IESYRTAYRSNPSHYYSGINALTLMYLYEDLTHDTRYHAEMSAMAGAIRFAAFVEAET
                     QPSFWPQATLGDLEILVGTPETVKAAYKEAIADNDNQGFTFHSSLQQLQLLNQLGFHP
                     ENVAAGIATFERALQRVTQAKAKLETPRQVFLFSGHMIDAPDRAKPRFPADKETSAAQ
                     HIANALKNRGAGPQDWALTQGACGGDLLFTEACQQLGVKVQWLQPFDEPEFIQESVVC
                     HSEAWRSRYLAAKAKLAFEIRAAPQALGPIPTDSDPYERCNLWLLYTALAHGTQKVQF
                     ICLWNGDGGDGPGGTAHMYNEVKRRTGQVTRIDTHTL"
     gene            18595..20037
                     /locus_tag="PKZ49_02050"
     CDS             18595..20037
                     /locus_tag="PKZ49_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HND35374.1"
                     /translation="MFSIRCIESTIRIMLPAAFLLVLTPLSADDKHGHANQLPNMFTS
                     TNAHGKDATYSTAGFIDLNNPFFKNLGSNGRSCASCHIPSQGWTITPQGVQAIFDKTA
                     GLDPIFRLVDGANSPTADVSTVEERREAYSMLLNKGNIRVGIGIPADAEFILVEADDP
                     YGFANENELSLFRRPMPTTNLKFLSTVMWDGRETTRDANSTDCIYGTAVCFSPVSFDL
                     STQSNHATLGHAEALRELTQEERDAIVAFELELFTAQIHDKKAGELTAEKGRGGPKKL
                     VKQDYYFGINDTLVGDYKTLAPFNSEVMSLYDSWNRYAPGNSHQVKSARAAIARGEDL
                     FNSKPIQITGVSGINDDLGVAVLPGTCTTCHNTPNSGNHSTPMPLNIGIADESRRTPD
                     MPLYTLQNKATGEIVKSTDPGRALITGKWKDIGRFKGPILRAVASRPPYFHDGSAANL
                     AEVVEFYNNRFSMGLTTQEKADLVAFLAAL"
     gene            20493..21944
                     /locus_tag="PKZ49_02055"
     CDS             20493..21944
                     /locus_tag="PKZ49_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35375.1"
                     /translation="MKVPFRSKTTYCAGILASLCFSQSVLADTLITHWNEAALQAIRV
                     THPGPPIVARALAITHTCMYDAWSAYDAKAKGTRLDDDLRRPDNEHTMANKSKAVSFA
                     AYRCLSDLFPSEVASFDGIMKSLGYNPADQSTDTNTAEGIGNVAAAAVIQYRHQDGAN
                     QLGDLNGGAPYSDYTGYTPVNSPSSIVDPNHWQPLQVGSNMQSFITPHWGLVEPYALK
                     SGDEYRKKIPAPANYHTEPARYRVQAQQVVDYAAHLTDEKKVIAEYWADGPLSELPPG
                     HWTLFATFVSKRDRHNLDQDVKMFFALTNAIFDASIVSWDAKRHFDYVRPVTAIHFLF
                     SGQQIPSWQGLIDGADWKPYQADSVVTPPFPEYFSGHSIFSAAGAETLKLFTKSDNFG
                     HSVVVPAKSSQVERNVVPDNDLVLFWATFSDAADEAGISRRFGGIHFIDGDLEARKVG
                     RVVAQNAWKKTLKYFGDKDHKHDKDDDHGKGKK"
BASE COUNT         6233 a         4505 c         5013 g         6370 t
ORIGIN      
        1 atgaagctgt aaaatatggt ttggttgatg cagtattaac aagtagagtt gtaggataga
       61 atggggcatt cggcgtgctt gatacaacta atctaatatc cgtgtaagac atgggtaggt
      121 aatttcttct gaggtgacag gatgtctgaa aaaatgaata gcgataagct actgtattgt
      181 tctttttgcg gaaaaagtca gcacgaagta aggaagctaa ttgctggacc ctctgtattt
      241 atttgcgatg agtgtataga tttatgtaat gacattattc gtgaggaaat gcaaacaact
      301 gaaacggcca aatttgtcaa atctaacttg ccagtaccga aagaaatttg ttcgattctt
      361 gatcaatatg taatcgggca ggagtctgca aaaaagattt tatctgttgc tgtatacaat
      421 cactataagc gcttaaggac gatatcgaaa actgtagatg ctgatgatat agagcttgcc
      481 aaaagtaaca tattattaat tggacctaca ggttcaggga aaacattact cgcgcaaaca
      541 ctagcgcgtt tattagatgt accttttatc attgctgatg cgacaacgct tactgaagct
      601 ggttatgttg gtgaagacgt agaaaatatt attcaaaaat tattgcagaa atgtaattat
      661 gatgtagaaa aagctcagca aggcataata tatattgatg agattgataa gatttcacgt
      721 aaatctgata atccatcgat tacaagagat gtttcaggtg aaggtgttca gcaagcatta
      781 ttgaaattaa ttgaaggaac aaccgcgtta gtgcctcctc aaggcgggcg taagcatcca
      841 aatcaagagt tcattcaaat tgatactacg aatattcttt ttatctgtgg tggtgcattt
      901 gatggtatag ataaaattat tcaggcccga tcagagaagg gtggtattgg ctttggtgca
      961 gaagtgatta gccgcaagaa tcagaaggaa atcaatgaag ttctaaaatc gattgaacct
     1021 gaagatttaa ttaaatatgg tttgatacca gagtttgtgg ggcgtttgcc tgttgtcgct
     1081 acgcttgatg agttgaacga agttgcatta attcagattt taacagaacc taaaaatgca
     1141 ctggtcaaac aatattggaa attattcaat atggaaggtg gtgttgagtt agagtttcgt
     1201 gatcaagcac ttaaggcaat agctaaaaag gcattactga ggaaaacagg tgcgcgtggt
     1261 ttacggtcta ttcttgaaga aacattgctc gacattatgt atgatttacc ttctctagag
     1321 aatgttcaga aagttgtgat tgattatggc tcagtgaata gtgatattaa accaatatta
     1381 atttattcag atcaatctaa atcagctaaa aatcccaaac aagcatagtt aagggccagt
     1441 ccgactggcc cttaacttct actatagatc tagttattgt tctcggtttt ctttctgagc
     1501 tttagtgtct ttttgataaa agggatacag agtaatccta gcaagatacc aataagcatc
     1561 acgccatagg tttgcagatt atcgctcgtg aactgagtta tttggcgaac catcttacta
     1621 gattctactt tatccccaac cttaggtaga ttttctgctt cgtaggtatc cgtcaaatta
     1681 taaggtgtta agccgggtat acttggaata tcagtaccac ctttacccac cacaagttgg
     1741 gctttcattc ctttttccat gtgatgcgaa atgtcacagt gaaccaggaa agtttcatct
     1801 tttccaggta cgatcatggt tcctgataca gttttagggc cagtcacttc caaatggaac
     1861 ataccaagct tatagagata ttttggtagg ccatgcatca tgaattggtg gcgaatatta
     1921 tcttcgttaa caaaatgcca tgtaatttta gaacatggtt ttaccttcca ttgttgttga
     1981 tcaaaggcaa atgctgtgcc aggaaactta gctgagtact ttctaccagc ccgtacagta
     2041 atttcgactt cctctgaaat actttgacag ctactcggca gtttatcaaa attctggccc
     2101 atgatcatcg tgccttcatg gtccataata tgatcatgat ccatatgatg gtgatggtga
     2161 tcatcagcac cactgacgct gtgttctggc gaatgagtgg tatgttggtt atggtccgta
     2221 gcatgatcat gttcgtgact tattgcatga tcactcgaat gctgatgctc actatgatgg
     2281 ctgtgatcat gattctgatc ggttgattgt tccttatgtt gctctatact gtggctacta
     2341 tgatcgtggt gagcatgctc atcattgatt gtatcttttg gctgtgctac tgcataggtg
     2401 ctagcagtca gagctccagc aatcaataag gataaaatat ttctattcat tattgattac
     2461 tccctttact tgcgtttatc cgtgaaattg ttccgagcat aacttgcgtg atagatttac
     2521 gtttagaaaa aatccagtaa aacatgagtc caaataacag accgatggct ccataaatca
     2581 aagaagtctg ggttttgtct tctaatccag atccttcccc aggagatcct aaactagacc
     2641 attcatcgag atcttgccat acgggtaatc tcctttcgta atattctttt gtgaaatatt
     2701 tgttcaggtc aattccaaag tggctaactt ttggcatgtt gctttcgccc aaataggatt
     2761 tatagacaat cgagctcata ctaccgcctt cagccatgcc attggtcgta attcctttct
     2821 cgcgatgatc gtggattaac caatctcctt caccatagct gtgcttacca tcgttgactg
     2881 tttccaattt taaatctagt cgttgagccg gagccatatc aaatacatca cgcataatct
     2941 gcgcaacggg attatgctct acaccatcat aatgagtaat cgttgcgtga tggccatgcg
     3001 tgtgaatggc aattaattcc ccaccactat ttaggaggcg gagtttaatt ttttgatcgg
     3061 gttcaaccac gattagggac tcccgcaatg tgtacggaaa ggagaggcca ttcagcgtgt
     3121 agtaatcctc agttgaatca gtaatgtcat agcgctgatt catatcgcgc gcaattaatc
     3181 taggatcgtt gtgccgctga atgatttgac ctaattcttt atccatcgca tgataatgta
     3241 aatcatattc ttgatcgtaa tgttcacgaa ctgctacgga tggatggcga acgtggccag
     3301 caccgatatt gagtgtctgt aaccagttat taggcttgtt ttcctcaata ataatcattc
     3361 ctgccaggcc cattgccaaa tgggtatgcg tttgcacatg gcaatgatag aacatcgttc
     3421 cgggttggcg agcctggaat tggtaaacac ggcgatcgcc aggcatcaat tcgatttcac
     3481 tcgtttgtgg cacgccatct tggcccccgt gttcatgatg tgcgaacggg tgatcaacac
     3541 catgaaggtg aatgctgtga ggaaaatagt gcgtattttc tagcacgatc tgaacaatat
     3601 cacccacttc aacccgaata accggggagg gtaagcgaag taagggatta gcgcgtatgc
     3661 tttcaaaatt ctcggttgac atgccgctca tcttaggtga aaacacccat gcacccggtt
     3721 gtataccagg agcaatatcg aaggttggta cgattccggg gaaatcgggt gatttaccgt
     3781 taagctccat gatctcaagt ctaatcgtaa tccggtcagg atcaccatcg ccatcaatat
     3841 catcagtttt gatgatagca tctggagata agcctgtttt catcaaagta tccatggata
     3901 tgttattggt acctttaact gctgctgcaa tccatgcagg attatctggg ctgcaaacgg
     3961 gtgaagcttc gatcttgaca ccttcaattt cttgagcttc acgccatgag ggcgcaatca
     4021 ctgggtcaca cattggttct gatgttaaag cttctgggtt tacttttacg gttgcaggta
     4081 attttgaagc gccgtaagta accgaactta acatgatgaa aggggctagc aatgcaataa
     4141 aaatataggt tggcatatta catactctaa attcaatgat taactaaata taaccctaca
     4201 ttatacagta gataccctag tgtggttttg ttggttttac caactgctag ctagcatagg
     4261 gatttaaccg agttaaattt aattaagaac gtagacaact tgtcgttcaa ttgaagtgat
     4321 ttcttcagct aaacataccg tgagtagcag caacttagaa tttagtttgg agaaaatcaa
     4381 aaatcaactt catgtacaca tttttacgat aaaggcgatc gattgatgtt agatcttgat
     4441 aaaaaacaat tcagtaaaca tttccgcacc attctagaga aaatagaaca aataccaccc
     4501 tttcctgaat cagctcggtt actttcagat cttcgacaaa atcctgatgc caatatcgat
     4561 caattgagtg aaattatcga aagtgatcct gggctcactg cttttattct aaaatatgca
     4621 cgcataccca tttttggtta tggccaccga attcatagtg ttgatcaagc tatcaggctt
     4681 gttctaggtt ttaatactgc aagtagcctt atgatgagta ttgcatcttc aagttgctta
     4741 agaatgcaaa agcatgggca gctaggctac atcaaaattt ggtcaaaaac cctagaatgt
     4801 gctgctttgt gccgagagct ttctttaatg atgagtaatc gatcacagat tgatagtgat
     4861 ttggcacatt tgtgtggatt attccagaat tttggctatc tgctatttgg tcatttcttt
     4921 ccaaaagaat atagctattt aaatgatctc gttgctcgtt atccagagca aagttttcgt
     4981 accattcaac ttcaattctt cggtatcaca catgatacga ttggaaaata cttgatgaac
     5041 gcctggaatt taccagagga agtgattgtt gcgattgctg agcaaaatta tccggattac
     5101 tcaggaaaac atgcgatcta tgtaaaattg attgcagtgg caaatcgttt actacagagt
     5161 gagttacaaa atattgatgg ctgtgcttat gtagaaacga cttcattaat tgacgacttg
     5221 ggtattaatg aaaacgaagc cttaaaagcc aaagaaaata tattaggctg ccaaaatgat
     5281 tttgcgattc tgagtcagag tttagcagcc taacagttat taattttcct agttcggttg
     5341 tggagcagaa gaggcattat caatgtcatc tgctattgct gctttcatac tcagacgcag
     5401 gcgtccttta tcatcagctt ctagcacttt aacgcgtact gattgtcctt ctttaaggtg
     5461 agcagcaaca tcttcaacgc gctcatttgc aatttgtgaa atgtgcaata gtccatcttt
     5521 cccaggaaaa atacttacaa ttgcgccaaa gtcgagtaat tttaaaacag ttcccacata
     5581 gatttttcca acttcaactt cagcagtaat atcttcaatc cgttttttgg cttgctcacc
     5641 accttcgctg ctgacacaag caatcatcac cgttccatcg tcggtgatat caatagtcgt
     5701 attggtttcc tcagttaatg cgcgaattac agcaccacct ttaccgatca catcacggat
     5761 tttttcggga tttattttaa acttaatgat acgaggtgca tgcgttgaaa ttgattctcg
     5821 gtttgcaggt aatgcatctt tcattatatt gagaatatgt aggcgacctt ctttggcttg
     5881 taacaaagca acctgcataa tttctttggt aataccctga atcttaatat ccatttgtag
     5941 tgcggtgatg ccattctcag tgcccgcaac tttaaaatcc atatcgccga gatgatcttc
     6001 atcgccgaga atgtcagtta aaactgcgaa gcggttccct tctttgatca atcccattgc
     6061 aataccagca acatgagatt tgagtggaac gcctgcatcc attagtgcaa ggcacccgcc
     6121 acagacagaa gccatagagc tagatccatt ggattccgtt atttctgaaa cgacgcgtaa
     6181 tgagtatccg aattcctcag ttgaagggag tactgaaacg agtgcacgct tagcaaggcg
     6241 accatgtcca atttctcggc gtttaggagt gccgacacgc ccaatttcac ctgttgcaaa
     6301 gggcggcata ttgtaatgca gcataaaacg ttcagagtaa tcgccttgaa gtgcatcaat
     6361 tttttgctca tcgcgagccg ttcctaatgt agccacaacc aaggcttgtg tttcgccgcg
     6421 ggtaaataat gcagatccat gagtacgcgg taataagcca tggcgaatgg ttattggtct
     6481 aacggtgcga gttccccgtc catcaatgcg aggttctcca tctaaaattt ggttacgaac
     6541 aatttttgct tctaaatcaa agccaatttc attaacgata tgttcgtcag ggccttcttc
     6601 agtgccaatc cctagatccg cataaacacg ttgccagaca gctttgattg cctcagttct
     6661 agcttgtttt tgcttgattt taaatgcatt acgaagatca gcttctgccg cttgtgtaac
     6721 tttctcgatt agagcgggat ctcgttcaac gggttgccat tcccaaggaa ctacgccggc
     6781 ttcatcagca aattcattga ttgcgttgat tacgatctgc atttgctcgt ggccatagac
     6841 gacagcgcct agcatgacgt cttcggacaa ctctgtagct tctgattcaa ccattaatac
     6901 agcttgttgt gttccagcaa cgactaagtt catctgcgta tttttgagct ctgttgttgt
     6961 tgggttgaga atatattcat tattgaaata acccacacga gctgcgccaa taggtccatc
     7021 gaaagggatt ccagaaataa ccaatgctgc tgaagtacct atcattgcgg gaatatctgc
     7081 atcgatctcg ctatctgaag ataataccat ggcaagaatt tgcacttcat tataaaaacc
     7141 agcagggaat agcggccgga tcggacgatc gatcaatcga gaggtcagtg tttctttctc
     7201 ggaaggtttt ccttcacgct taaaaaagct accaggaatt cgacctgcag aataaaattt
     7261 ttcttgataa tcaacagtta atgggaaaaa atcttgacca ggttttgcat ttttatcacc
     7321 aaccacggtt accaatacaa ccgtttcccc cattgtaacg atcacagcgc catgggcttg
     7381 tctcgcaatt tcaccggtct ccaatgtgac ctcatgacga ccgtaggtaa agctcttttt
     7441 ggttaatttc aattaacgat ccttctatat ttgtatactc gtttttcttt agttatgtaa
     7501 caaaacaaga atcaaacgct caaaaatctt acacttacgt aattattccc acccgtttca
     7561 tactaacaat aaattctagt cactaattat tttctgagac ttagacgctc aataattttg
     7621 agataacttt cactattttt tcttttcaaa taatccaata acttacgtcg gtgattgacc
     7681 attttaagca atccgcggcg ggaatgatga tccttgacat gcgctttgaa gtgaccaatt
     7741 aaatcattga tacgcgttgt taaaagtgca atttgcactt caggggagcc ggtatcacct
     7801 ttgatccttt gatagtcact tatcacctgg gctttttgct ctgttgtgac tgccataaaa
     7861 ttttctccag ttcagttaaa aagtagctta ttcaattacc tatcatctgt aaatgaaggc
     7921 gaaattctac tctttaatcc atattttgtc caaaaagggt gaatatcaac tttgacttcg
     7981 atttgttcta catcaatttt tactaaattg tttgtaatga ggataacaga taaactgagc
     8041 gccctattgg gttaagtttg taaaatagaa attaaggaaa acgtatgaaa agaattttgc
     8101 tactcgggat gctattgata cccttaataa cgatggctga agatttgagc ttgcggcaac
     8161 gcgcaaatta tgcgtttgag caaatgaaag cagcagaacg agatttaaca accattactc
     8221 aaacaattga agttaagaaa ggccgtttgg aatatttcaa acaaaaagca atcgaagcgg
     8281 aacaagaatt agcaaacgct tacaaagaat cgcaagaaat caataaacga gtaacatcga
     8341 cccggaatcg ttgggaacaa ctttccaatc aattgttaca aggtgatagt gaaagccgcg
     8401 attaaagtaa taatggccta acttgttgta acgcatcgat cgatatcaag acaatttcca
     8461 caatagccta tttggggaaa tataagcaga tttccgttct tgccctgctt cagtagcacc
     8521 tcttttttcc ataccgagct aagcttctat tgctcattca tctgatctct tcagttgatc
     8581 aaaatcatac accgcgattt atttttctgg catgattgct gagaggtcgg ttgaatcgaa
     8641 taggcgctat ttctacgtta ccatctcagc aatggttagg ctgtatgtga aacaccataa
     8701 aaatacacag gagggcgata tggctaaaaa agcactttgt attggaatca ataattatcc
     8761 aggaacacac atggatttac agggttgtgt caatgatgca caagactgga gcaatgtatt
     8821 gcagtcgcgt ggatttgtga ttagcgcttt actggatcag caagcaacca aagcagccat
     8881 gatgagtgca atgagtaatc tcatcaatcc tgcggtggca ggggatagct tagtcatcac
     8941 gttttctggg catggcacct atcaacctga taaagatgga gatgaagtcg atgggttgga
     9001 tgaagcgctt tgcccttatg atattcaaac taaaggggaa gccttaacgg atgatgaaat
     9061 tcacacgctc tttaaagtgc gcaaaccggg tgtcaagcta gtccttattt cagatagctg
     9121 tcactcggga acagttacac gtgccgcagc agcggaacct gaagccgatg caccacgccc
     9181 tcgttttatg ccgatgggca actggatgtc tgataaggat ttgccgcaca gtcggagcgg
     9241 cggtattgcg ccattcatca atatcggtgc gagtacttcg cctttcttgg cggcttatac
     9301 aagaagtgag gatgatttat tgctttcggg ttgccaagaa ggccccaaca attttagcta
     9361 tgatgcaaaa attagcggac gctacaatgg tgcatttact tattatgcgc taaaaaccct
     9421 taaaacatta agtgtcaatg caacgtataa agactggcat gatgcgatta ggcggtattt
     9481 accttctgct agttatcctc aaactcctca actggtagga acattaactg cgcgccaaat
     9541 gaaagttttt tcctagtaat tcagcgaatt attaagtgca gataagaatt gtagattcta
     9601 aatctactga gcttagatga tcggaggtag agtaatgagc gttgatgaat accaatatcg
     9661 tgtcatagga atagaacaac cgacagtcgc ttccccgcaa gttaaggaag tcaaggtcat
     9721 taaaaaacga agcttggtgc ccggcggtac tcgatctgcg aatgtgggtg actttgatat
     9781 taaagccaat gaaatcgttc atcttaagtt aaacaatggt ttttccttgt ggctgcgtgc
     9841 agatgattta ttggatgagt tcgggaaacc gaatattagc cgtggaagcc agcatacttg
     9901 ggaaatcaga gtcgacccag cgaaattcca agctaatcga agcgcacgag gtttggcagg
     9961 gttagccatt aaggtactcg atttcttcga tgtcgatctt caatcctata ctgcaagtaa
    10021 gctttgttca ttatgggaag ataaacttct tgagaagaaa gcagctaatc tgtatcgatg
    10081 ttccttagaa gatacttacg cattaagcga gaagggggaa ttaaagtacg aacagccttt
    10141 actgctcttc attcacggaa ccgcttcgaa tagtcagaaa agctttggtg gattgtggga
    10201 agcgtcttca aaagtaggta aagcagcgcg ggagagattg aagaaaaaat atggtgaagc
    10261 agtttatgct ttcgaacacc gtacattaac agaaagccct atccagaatg cgctgcaatt
    10321 agtcaaacaa cttccaaata atgcaaaact ccatttgatt acttactcac ggggtggatt
    10381 ggtgggtgag ctactttgtt tgggggattt aaaatcaacc ttacccgata cagaaacaat
    10441 ccataatcta tttgcagccg atcgtaccat cgcgttacag cttggattaa atcctttgga
    10501 tgaaagcgcg caaaaagaaa gaaacaaagc ttatgacgat gatcgcgaag cactgcaaac
    10561 gttactaaaa gaacttaagc aaaggcaaat caaagttgaa cggtttgtgc gtgtggcgtg
    10621 tcccgcaagt ggaacgacat tagcttccgg tcgtctcgat cgctggttat cggcgttaaa
    10681 ttatctatcc ggggatggat tgtttggcga tactgtcgat tttttactgg cggtccttaa
    10741 ggaacgtact gatccccgaa cactgcccgg gttagaagcg atgatgcccg gttctgcatt
    10801 gacgcgctta ctccaagatc catcgttaga aaccaatgcg gatctttcta ttattgcggg
    10861 cgatattgag ggtgattcgg tatggagtca gctaaaacta tgggtgactg actggtttta
    10921 tggcagtgat catgatttag tagtgaatac cggttccatg tatggagggc tccgtcgctt
    10981 accccaacaa gctcgttttc tctgtgatcg aggtccagct gttcatcatt tcaactattt
    11041 taacaacgac aaaacgatta attggttgct gcagggattg gaacgtctcg atggttctaa
    11101 tggtccattt caggctattg agcaagccaa gccagaacaa ccgcgttggc gatctgcagt
    11161 gcaacgcagc cgtagttctg caacgccacg tcccctggct gttgtattgc cagggacgat
    11221 gggaagcacc ctaaaggttg gaaaagaaga agtatggtta aattattgga cgcttgcaca
    11281 gggtggatta aaaaaaatac agatgggtgc gataggagtt cagcccgatg atttattaga
    11341 tgatttttat ggtccattgc ttgagttttt ggcgctgacg catcgcgttg aaatcgttcc
    11401 ttatgattgg cgtctttcaa tccgagaagc ggcgaaacgc cttatcgaaa cattgcaaga
    11461 acatttaccc gaagcggaac gaaattatca accagtacat ctgattgccc attctatggg
    11521 aggcttggtt gtaagagcga tgctagcgga tggcaaggaa ggcactgcgt tatggcagcg
    11581 cattgttaaa ttacccaaaa gtcgcttcct aatgctagga acaccgaacc agggttcgta
    11641 tgaagccgtg cgctggttga ctggcaataa tgcaacacag gctaagctga gcttgctcga
    11701 tctgacgcaa aatacggatg agattgccaa tctagtacgg cgctatccgg gtttattaga
    11761 attgttaccg tttgatccag acagtaaaga ttttacccaa tcgaatcttt gggctgatct
    11821 taaagcgcaa ttatcggcag catggctgcc accggaaaca cttgatctcg tcaatattcg
    11881 cgatacttgg tctttgctta agaatccagc gcagtcggtt gatccaaaat atacctgtta
    11941 tgttgctgga tgccaggatg caaccgtagt ggattatcgg ttagtcgatt gcgggggatg
    12001 gctgcatgcc aattcaaatt gtaagcgttt tgagttcttg gctacccggg aaggagatgg
    12061 aacagtcagc tggaaatcag gtcggttacc cgatgttccc ctatggtatt tggaagatac
    12121 tgcccatgat gatttatgca ggcagaaacg cgcttttccg ggttacctgg atttgttgat
    12181 gaatggagat acttcgcatc tatcaaaaac tccacccggt cgtgcgcgtg ctccagtggg
    12241 tgagcctgaa ttgtttgccc tgccggccta tccggttatt gatagtattc ccagtgaagc
    12301 aagtttgcgt aactttgggt ttggatcgtc acgaccgatg cgtgagatgg aaagtgggct
    12361 gacaataccc atcattgaag tcagtatttg tcacgggaat ctagcctatg ccaaacatcc
    12421 cgtgttagtg gggcattatc gcggagatgg gatttttagc gctgagaaag cattggatga
    12481 gcgtctggag tgtcagttat caacccggat tcggctagga ttatatcccg gtgcaatcgg
    12541 tagccatgcg ttgttttatg ccaaagaacc gaatgccaaa cccagtgggg cgattgtaat
    12601 tgggttgggg caagtgggag atctgacgcc gggattattg acccttggtg tccagaacgc
    12661 gctactggaa tgtgcattac agattgccca atggccagcg gaagataccc gattcagcac
    12721 tgaagctgga aaacgttcta ttgatatcac gtcgatgctg gtcgggactg gcgcgggtgg
    12781 gatgaccgtt cgggattcaa tcgaagcgat tcttcgcggc gccattgctg ccaacgagaa
    12841 attagcagaa gctgagttag tcgatcaagt ggcgatcaac cgtttggagt ttcttgagct
    12901 atatgaggat atagcaattg cagcggctga ggcactcaat ttgattttgg cgaataatca
    12961 attatccaat catgtcaatt ggccagacgc taccgtgaga gcgggggagg gtggttatac
    13021 tcgcctacgc tatgatgaaa tgggcggttg gtggcatcgg atggaaatta gcgaaactga
    13081 agggcgggaa atgctgcggt ttgtctcaac cacggatcgg gcccgtgcag aagaaaccct
    13141 tgcaacaggc cagctcaaac ttgctgataa ttttattcgg caagcgtccc aggatgcagg
    13201 tgccaatagt gaagcagcaa agacgctatt tgaaatgttg ctgccaaatc gtcttaaaga
    13261 attagcgcca caacaggaag ggcttgtttt aatcgtggat gaatcttcag cgcgttatcc
    13321 ctgggaatta ctcgaaaatc gctggagcca taatggcaaa ccaccggccg ttagcgctgg
    13381 attaattcgc caactcaaaa catccgtatt tcgcccttcg cccgtacata ccacaaagct
    13441 caatgctttg gtaataggta atcctaacct ttctgggtgg agtagttttc ctgatttggt
    13501 tggggcacgc gaagaagcgc agaaagtgag tgaactattc aatgggaatg gttttcaagc
    13561 catcgattac atcgatgcca atgcggacga tattctttca gggttgcata aagatgaatg
    13621 gcgtgtgctt catttggcag gtcatggtgt tcatgatttt gacgtcagca aggcagattc
    13681 cagtgcgcta gatctgctag ataacgctac tcaatcagta tatagcaaga aaaaattgat
    13741 ctcagggatg gtgattggaa aaaatacatt tttgacacca ggcgatatcg agcaaatgcg
    13801 ttgggtgcca gaattagtat tcatcaactg ttgttatctt ggtaaaaccc aatcggtgat
    13861 tgagaaaccc tatggtgccc tggccgcaaa cctggccgca caatttattg tcatgggcgt
    13921 taaagcagtc gtttcagcgg ggtgggcgat caatgatcaa gcaggtttag cttttgcaga
    13981 cagtttttac cagaagatgc ttctagggat gtcgtttggc gatgccgtta ggcttgcacg
    14041 tgaagaaaat tggaacaatt ttaaaacgct caatacgtgg ggtgcatatc aatgttatgg
    14101 tgatccagat taccgcttaa tgaataaagg aactgactta ttagcacctt ccactaagaa
    14161 aaaaacctat tatgcacccg cagaattgtt aaccgatttg aagaacgata ccgcattcat
    14221 tcgcatgcaa ttgaatgata agcgcaaaac agaagcggaa ttgagtgatc tgaagcaaat
    14281 gatcgatggt agattaacag caattcctca agataagcaa aaggcctggt tggcccgcga
    14341 agatgtgaag attgcattcg ggcttgccta tggtgaatta cgtgcatggg atgaagccat
    14401 tcagtatttg gaagctgcgt tacaagcagg taatgctgaa tgttccatta aagtgatgga
    14461 acagctcact aacttccgtg ttcgcgcagc aagtcagcga tggagtcagg ttgatgcggt
    14521 aacagacgca aaagcccaac accaatgtcg aactgatatt gagaaagcaa taaaagaact
    14581 agaactttta tgtgaattct tcccaacatc agagcgatat tcgattatgg gcagcgctta
    14641 taaacgcctg gcccgcatac agaccaacaa aaaatccctg caaaatgcgt tagaaaagat
    14701 ggcgctttat tatcgaaaag cttatgaaaa aagtggatcg actgattact atccttttac
    14761 caatatggtg actgcaaatt ggctcatcaa gaatttaggg aaaccgtctg gtaacgattt
    14821 tgatgcgtta atcaacgatt gtgatcgaat aatcgagatt actcaagaga aaaacagccg
    14881 tagccctaat ttctggaata gcgtagcgct ggcagattgc gctttaatca aatggatgct
    14941 gcgaggggaa ttatcggaag catcaactaa cgaagtgatc acgctttatc agcgggcaat
    15001 tcaaagaggg gcaagcccac gggagatcgg gtcggttaag gagaatttcc aattcctcat
    15061 ggatttgacc caatcacttg cgcaaccgat acatgaagca ataaatactg tttttgagtc
    15121 attgccttga ataagttgtt aaaagcgtaa accttgctag aaaaagatac ctaagttagg
    15181 attactttga aatagtaaat cagggctttt cattgcgatt agtgtttgag cttgcaggtg
    15241 tttctgattt gctcgtagct gctgcaaagc cagtactttg ggtagtttgc tgaggaacct
    15301 gttgtatgcc tggtaccgga gcgcctttct gctctgtttt gtgcgtctgc tgaatagtag
    15361 gagttggcgc atcttctttt agagatgctt gttttttgtc gaccatgatc gctatccttt
    15421 caagattgat tgagacaaaa attgtactac atacaattgc gataacgaat gtgaaaccag
    15481 cagcataagc gagccagtca gtatatcgtg cgcagcaatt cttttccgaa agggcagatt
    15541 cgtccatttt gaggtaatac cgatcattga tttcgagctg ttttttgatt ccttgctgtg
    15601 atgaaataaa cgaggataga gtgattacaa ctgccaaact gaaacaaccc catgacaaga
    15661 acaaaagcca tgcagcacag gcttgagtaa taggaatgaa gtcctttaga aagccaagag
    15721 atagtgcaag accagcagat gaatatgtga gaatagcttt atcgaagttt tccgagtttg
    15781 agagttgacg tttaaataac tctgttctta gctctgcatg aactttcttt cgttcttcta
    15841 caacatcggt catatcaaca aatcctaact agaatgagat atctgaaatt tccaatttgt
    15901 ttggccataa atggaattta atacaatcct atttaacata tttatcagtg ttttaaacaa
    15961 tacctcattc aacagctatc atattagacc tttagatcta caaaagaatg ggtatgagga
    16021 aagagatagc agttacagcg tatgagtatc gatccttgtc acctgcccgg ttcttcgctt
    16081 gacttcatta tacatatgtg cagttccacc ggggccatca ccaccatctc cattccacag
    16141 acaaataaat tgcactttct gggtgccgtg cgcgagtgca gtataaagca accaaagatt
    16201 gcagcgttca tagggatcgc tgtcagtcgg tatggggcct aatgcttgag gggcagcgcg
    16261 aatttcgaat gcaagtttgg ctttggccgc taaatagcgg gatcgccagg cttcgctgtg
    16321 gcagaccact gattcctgga tgaattcggg ttcatcgaaa ggttgcagcc attgtacttt
    16381 tacccccaat tgctgacatg cctcggtgaa taataaatca ccgccacaag caccttgtgt
    16441 gagtgcccaa tcctgaggac cagcgcctcg gttttttaat gcgttcgcaa tgtgttgcgc
    16501 tgcgctggtt tctttgtccg ccgggaaccg cggtttggct cgatcgggcg catcaatcat
    16561 atgaccgcta aacaggaaaa cttgacgggg tgtctccagt ttggccttcg cttgagtgac
    16621 gcgttgcagc gcgcgctcaa acgttgcgat gcctgctgct acgttttcag gatggaaacc
    16681 caattgattg agcagttgca attgttgcaa actggagtgg aacgtaaagc cttggttgtc
    16741 attgtctgca atcgcttctt tatacgcagc tttcacggtt tccggggtac ccaccaatat
    16801 ttccaaatcg ccgagcgtcg cttgcggcca gaatgaaggt tgagtctccg cttcaacaaa
    16861 agccgcaaag cggatcgcac cggccatggc gctcatttcg gcgtgatatc gagtatcgtg
    16921 agtgagatct tcgtataaat acatcagggt tagcgcatta attcccgagt agtaatggct
    16981 ggggttagag cggtatgcag tgcgatagct ttcgatagcg ttttttagta atggcgattg
    17041 gtaagcggct tcgtcgcggt aactttgtgt cgtttgattg ggctgatacc aggcgctata
    17101 ccacgcatct ttgtcgatcc gacccagcaa tgcccaagtt tcagcgtcgt gctgatagct
    17161 ttcctggctg aggatgcttt ggtaatgcgt gcgcgcccga tctaaagaat agccagattc
    17221 gttgcgctgc gctaagcgtt gcaaacaaat gcctttttcg tgtaacccca gtagattatc
    17281 cggttcaatg gcaaggccac gttcgagttg atcgagcgcg aagtggaaat gctcatgttg
    17341 acgaagcgct ttgcccgctt taatccaggc ttctgcacgt aatgccgcga caggcgcttc
    17401 atctgcgagc accaacacat cccccaggta tccagcgttg actgcaagct tgattcgatt
    17461 ttcccaagct tggtatttct cccagtattc ttggataccg ccaattcgca gcgatttcca
    17521 atccggttcc tgaagatggg gcagtagttg ataaacaggg ctggttttgc ggccatgcca
    17581 tgattgcatg gtagctttga ccatttcggt caagttttga atatcagcac cgactaacag
    17641 gggatctggg ccttccgctt tgatgtggta gcgaaatttg cgatccgtat agagatcaaa
    17701 cgcagtggaa atctcaccac cacaaattaa caccacaccg cgtgcccgca ggccatgccg
    17761 tatccctagc tcgtaccaaa cattgggatt gtcgatcgtg atatccgcga tgacgagatc
    17821 ggccagtaaa agctcttgaa acatatccgt gcgaatatca ccagcacgat gctcttcatc
    17881 ggctctgaat ggctgtaagc cagcagcgcg cagtgctggt tggatgagtt cacgatagac
    17941 acgattgaaa tcgattagat taccttggct atcttttttg gtaccaaagg gcattgcgac
    18001 aaacgcatga ggttgcatgg ctggcctctc ttaaatgata ggggtggtga tagtttactg
    18061 gaaggaaagg aatcgttaaa cgcaacagaa atagaaagat tgaaaacatc tctactcggg
    18121 tcagtttcca agtatgtatt atgcgcttcg tgttgtttca atgtgtcttg taatctttta
    18181 taaaaatcaa ttttatattg gtattcgtcc tgtactaagg ggccaataga tgaagcaagc
    18241 gattttcgac agaattcggc gtgttggtta ctgatgatag tcaatcttta tgtaagcaac
    18301 ttaaagggtt cttacaaaat ctgcgcatgt gttttttcac acagacccta tgaatccgaa
    18361 ttagtaagat ttttatcggc agtgggcaaa ttagaaattg aatttgtttt cgatgcctac
    18421 agttgatgta tcttatgact gaaaattaat tcttataagg gaagtgaaag cagactcaag
    18481 tgataagtgt aatcaagtga tgcaataaat aaatcaactt gctgttctca aaataattag
    18541 gtagtaaatc taggtaattt tcttaaacta atagacaaat aaacaggaga tcaaatgttt
    18601 agtattcgtt gtattgaatc gacaatacga attatgttac cggctgcatt cttgctggtg
    18661 ttaacgccgc tgtcagcaga tgataagcat ggacacgcta atcaattacc taatatgttt
    18721 acctcgacga atgcgcatgg aaaagatgcg acatacagta ctgcaggttt tattgatctc
    18781 aataatccat tttttaaaaa cttggggagt aacggacgta gctgtgcttc atgccatatt
    18841 ccttctcagg gatggactat tacccctcag ggcgtgcaag caatttttga taaaacggct
    18901 ggactcgatc ccatttttcg tctggtagat ggtgctaatt ctcccacggc ggatgtttcg
    18961 acggttgaag aaagacgcga agcctatagc atgctgctca acaagggaaa tattcgggta
    19021 gggattggta ttccggctga cgccgagttt attttagtcg aagccgatga cccctatggc
    19081 tttgcaaacg agaatgaact ttcactgttc cgtagaccca tgccaaccac taatcttaaa
    19141 tttttaagca cagtgatgtg ggatggtcgt gaaacgaccc gagatgctaa ttcaactgac
    19201 tgtatctatg ggacagcggt ttgtttttca ccggtatcgt ttgatttatc tactcaatcg
    19261 aatcacgcta cattagggca tgcagaagcg ctaagagaac taacgcaaga agagcgtgat
    19321 gccatcgtgg catttgaact ggagttattt acagcacaga tccatgacaa gaaagcgggg
    19381 gagctaacag cagaaaaggg acgaggtggg ccaaagaaat tagttaaaca agattactac
    19441 tttggtatca acgatacgct ggtcggtgat tataaaaccc ttgcaccttt taattcagag
    19501 gtgatgtcgc tttatgatag ctggaatcgt tatgcgccgg gtaacagtca tcaagttaaa
    19561 tctgcccgcg cagccattgc acggggtgaa gatttattca acagcaaacc gatccaaatt
    19621 accggtgtca gtggcattaa tgatgatcta ggcgtagcag tattgccggg tacttgcact
    19681 acttgccata atacccccaa ttctggaaac cactcgacac cgatgccatt aaatattggt
    19741 atcgccgatg aatcacggcg tactcccgac atgcctttat atacgttaca aaataaagcg
    19801 acgggtgaaa tcgttaaatc aacagatcct ggccgtgcgt tgattaccgg gaagtggaaa
    19861 gatattggtc gttttaaagg accaatccta cgcgctgttg catcacgccc accttatttt
    19921 catgatggtt ctgctgctaa tttagcagaa gttgtggagt tttataacaa tcgctttagt
    19981 atgggtttaa caactcaaga gaaagcagac ctcgttgcat tcttggctgc gttgtaattc
    20041 ttatcactcg ttattactgg atacgctagt tcatagggta tccaggatgc ttgatacgat
    20101 caggtgatcg tatgtactag ctaacaactc gctcctgaat ctctgaagag agctcatttg
    20161 tttttttatt acgctatcca ctcaaaaaat agcgcttcct gcaattcata ctactttctc
    20221 ttggaaagta gtatgtacca atgaccaata gatgaagtga cagcctttcg acagaattca
    20281 atgtgttgat tactgatggc agtcagcctt tgtataagca ccacggaagt tttttacaaa
    20341 ggatcgactc atgtgatttt tcacacagat tctatcaatc ttaatttgta agattttcac
    20401 tggtagcgag caaactggaa attcaatttg ttttcgatgc ccacaatgta aataccttct
    20461 gactgaaaat taattcttat aaaggggaaa aaatgaaagt tccatttcga tctaaaacaa
    20521 cttattgtgc tgggatctta gcatcgcttt gctttagcca atctgtttta gctgatacgc
    20581 tcattactca ctggaatgaa gctgcacttc aggctattcg cgtgactcat ccaggtcctc
    20641 ctatcgtggc aagagcactg gcgataactc atacctgcat gtatgatgct tggtctgctt
    20701 atgatgccaa ggcgaaaggc acgcgactgg acgatgattt acgccgtcct gataatgagc
    20761 ataccatggc taataaatct aaagcagtga gttttgcagc atatcgttgt ctatctgatc
    20821 tcttcccatc ggaagtggcc agttttgatg ggataatgaa atcgcttggt tataatccag
    20881 cagatcagtc gactgatacc aataccgcag agggaattgg gaatgtggct gccgctgctg
    20941 ttatccagta tcggcatcaa gatggtgcaa atcagctcgg agatttgaat ggcggcgctc
    21001 cttatagtga ttatacaggc tatacgcctg tcaattcgcc ttcttccatt gttgacccca
    21061 atcattggca accgttgcag gttggttcca atatgcagag ctttattacc ccgcactggg
    21121 gccttgtgga accttatgcg cttaaatcgg gtgacgaata tcgtaagaaa attccagccc
    21181 ctgctaatta tcataccgag cccgcacgct atcgtgtgca agcacaacaa gtggtggatt
    21241 atgcagcaca tttaaccgat gaaaagaaag tcattgctga atattgggct gatggtccgc
    21301 tttctgaatt gccacctggg cactggacct tgtttgcaac atttgtctca aaacgtgatc
    21361 gccataacct tgatcaagat gtaaaaatgt tttttgcgct aaccaatgcc atttttgatg
    21421 ccagcattgt ttcttgggac gcaaagcggc attttgatta tgtacggcct gtcacggcaa
    21481 ttcacttcct tttttctggg caacagattc catcttggca agggttgatc gatggtgcag
    21541 actggaaacc ttatcaagct gatagtgttg ttacgccgcc attcccagag tatttctcag
    21601 ggcacagtat tttcagcgca gctggcgcag aaacactcaa attatttacc aaaagcgaca
    21661 attttgggca tagcgtcgtt gtgccggcga aatcatccca ggttgagcga aatgtcgtcc
    21721 cagataacga tctcgttttg ttctgggcga catttagtga tgcagccgat gaagcgggta
    21781 tttcgagacg gtttggtggg atacatttta tcgatggcga tcttgaagca cgtaaagtgg
    21841 gtcgtgtcgt tgctcaaaat gcgtggaaga aaaccttgaa atatttcggc gataaagatc
    21901 ataagcacga caaagacgat gatcatggca aaggtaagaa ataatagcgt tagtactagc
    21961 ttgctaagtt aagcttattg catatacgta accgtaacca actgcctcat cattcatgat
    22021 gaggcagttg gtttttagga atctcttcat tttacaacca tgagcgagcg actcacataa
    22081 gcgacaaaaa tgattgcaca acaatagggg ccaattaatc c
//
LOCUS       DAOQKQ010000017        21609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_1045_length_21609_cov_6.350515, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000017 DAOQKQ010000000
VERSION     DAOQKQ010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 21609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21609
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1045_length_21609_cov_6.350515"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            349..2781
                     /locus_tag="PKZ49_02060"
     CDS             349..2781
                     /locus_tag="PKZ49_02060"
                     /inference="COORDINATES: protein motif:HMM:NF040115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35376.1"
                     /translation="MSIQGFTPAQIAAFTTSQIQSLTTDDFKKLSTAQINALTSEQIG
                     AIETEDLSVLSATQIKSFDAQEFADGMLTYQIEALSQTHIAALSTDQLSVLTTDQIQA
                     IETRDLIKLSAAQLNALSTEQFNNLSSDQIQSLTATQIKGLSTDQLSTLTTDNVQLLT
                     GAQIASLSITQLNSFDSDQIAAIDVADVAAFTATQVKGMDSSQIAALTTSQITQLSNN
                     QLAALTTTQIANIETDDLAAMTSSQITAFSAKQIVAFTSDQLNNMTSDQIQALTATQI
                     KGLTTDQVSSLTSDNIQLLTSAQVAALSTAQLNSLDSDQIAALDTGDVAALTANQIKG
                     LGTTQIQDLTSDQLQALTANQIKGLTTDQIANIETDDLAAMTSSQIAAFSAKQIAAFT
                     SDQLNNMTSDQIQVLTAAQIKGLTTDQMSSLTSDNIQLLTSTQVAALSTTQLNSLDSD
                     QIAALDTEDVAALTATQIKGLGTTQIQDLTSDQLQALTANQIKGLTTDQIANIETDDL
                     AAMTSSQIAAFSAKQIAAFTSDQLNNMTSDQIQVLTAAQIKGLTTDQMSSLTSDNIQL
                     LTSTQVAALSTTQLNSLDSDQIAALDTEDVAALTATQIKGLGTDQVVALTTSQAAALQ
                     YAQIAALSVNQIAVFETDDLAQLSSDNIKALSSLQIRALTTDQIDSLSTEQIQGLNAA
                     QIAAMTSEQISTLGTEDIQALTTAQISALTSEQISGLTTEHISNLESEDLQALSAIQI
                     KLFNTDQITNGLSTTQIQQLTTTQVAALTTDHIPNLSTDQIDALSTDQVRALTSDQIA
                     VLTTDQIPYLHF"
     gene            2871..5105
                     /locus_tag="PKZ49_02065"
     CDS             2871..5105
                     /locus_tag="PKZ49_02065"
                     /inference="COORDINATES: protein motif:HMM:NF025214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HND35377.1"
                     /translation="MYDQPHSDYTAWIQGIWQRVVKFSDLITYANQKNFPSSLAAVLY
                     QTWLKRNHTTDDVFAWFNLGVSLFSEKDFIGAREAYEAALAIKPNFIAARFNLGLIYE
                     RLNNTQAALAQWKQVEETANSAVPDDLAILVLALNSTGRLQETLKQYQLACEALEKSL
                     LLKPNQSDVIHHLIFMRQKQCRWPIYESVGATGESALRKATSALAMLNISDDPYTQLE
                     GALNYSRAKIPTDLPRLAPPNGYQHKKIRIAYCSGDFCTHPVAMLTVELFEQHDKDKF
                     EIYAFCWSPNDGSNLRQRIIQGVDHYIPIHELNEADSARLIREYEIDILVDLHGQTSN
                     ARIQMLAHRPAPIQITYLGLPATTGLPCIDYVIADRFLIPQEYANFYSEKPLYMPDIY
                     QVSDRKREISSAPTRESCGLPKNKFVFCSFNNNHKYTLEVFTTWMNILRRVPESVLWL
                     LADNPWAKSNLKKQAELQGIDPTRLIFAKRTLPADYLARYHVADLFLDTFPFNAGTTA
                     NDSLWAGLPVLTLCGRSFASRMAGALLTTADLPELITYDLKSYEDKAVELAQNPKVVK
                     KLRKKLADAKINSPLFDTTRFARNLETHYTNLVAAFNYQASSVQSAKQAASVSEQPQK
                     LKQLTDIVTKAEKLQAQDDIISAIQTYHQWIKYSCSQDKWIAWFNLGALLSSSGDLTG
                     AEQAFQVVLQQQPGFIQARKALEVLHSCMPKSKQLCSENDKNALDFWKCVTSETKKPL
                     VAHS"
     gene            complement(5211..5541)
                     /gene="tnpA"
                     /locus_tag="PKZ49_02070"
                     /pseudo
     CDS             complement(5211..5541)
                     /gene="tnpA"
                     /locus_tag="PKZ49_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_044282811.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(5589..6179)
                     /locus_tag="PKZ49_02075"
     CDS             complement(5589..6179)
                     /locus_tag="PKZ49_02075"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HND35378.1"
                     /translation="MKKVLNVGGNNKSIPLPPQYVEFEHLLLDIDPRGEPDFVCDARN
                     LATLDADQFDAIYCSHNLEHYYRHDVKKVLNGFLHVLNENGFVHIRVPDIQEVMRITM
                     ERNLDLDDFLYQSSAGPIMVSDVLYGYSVEIERSGQDFFSHKTGFTQKSLEKALQNAG
                     FYKIYTTTRNLEIVALAFKGVPDSYVSSLFDLPLLS"
     gene            complement(6570..7442)
                     /locus_tag="PKZ49_02080"
     CDS             complement(6570..7442)
                     /locus_tag="PKZ49_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA4780 family RIO1-like protein kinase"
                     /protein_id="HND35379.1"
                     /translation="MKTPKQIESLIEEGLVDSVICQLMSGKEALVYVVQCGSELRCAK
                     VYKETHRRNFRHSANYTEGRYLKNFRRTREIQKRSCFGKVAREEAWQNTEINALCRLS
                     AAGVKVPRYINFFEGVLLMELITDSQGNVAPRLNDIRLTTEQAKSYHTLLINQVVRML
                     CRGMIHGDLSEYNVLLGSQGPVIIDFPQAIYAVENNNARWLLKRDIDNITKYFSHFSP
                     ELAKTDFGSEIWSLYERGKLDWDTPLTGVLVETHKPVNIDGLLKTIDSVMKKELAWQR
                     YKQERWTSQRSLAF"
     gene            complement(7484..7669)
                     /locus_tag="PKZ49_02085"
     CDS             complement(7484..7669)
                     /locus_tag="PKZ49_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35380.1"
                     /translation="MTLKHNQHGVSSHSSKVTIGTSEFGENKPQTKSIKQNTTTHLSQ
                     MEDASWHRSKSARFQQN"
     gene            7878..9029
                     /locus_tag="PKZ49_02090"
     CDS             7878..9029
                     /locus_tag="PKZ49_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HND35381.1"
                     /translation="MDFDEENLFPHQDALAQLHENRPLSEKLNFLHQLIREDFPFIDR
                     LAIALLDEKTRTLKTYTHSTDDGNSPITAYEASLDSAPSLLEIIKRRRPRLVQDLAIF
                     NKGAHQHTKLIAAKGYKASYTMPLYQSGVFVGFLFFNSYESHPFLPPILRQIDIYGHL
                     IALMVISELTAIRTLLSAVKAARSMTHYRDVETGSHVDRTAHYARLIAKELAQHYQLR
                     DEQIEHIFLFSPLHDIGKIGIPDSILRKPGKLDDQEFAVMKTHPGKGREIIDSILKDF
                     SLGTFGHVEILRNIAEYHHEAIDGSGYPNQLQGNEIPIEARISAVADVFDALTSSRTY
                     KAAWSNEEAFAMLQRLSENKLDKDCVEALIKNKDKILEIQQLFRDSEDK"
     gene            9083..11488
                     /locus_tag="PKZ49_02095"
     CDS             9083..11488
                     /locus_tag="PKZ49_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HND35382.1"
                     /translation="MSTPIQTNTLNSTQAKIISLIATELTAKTPQVSAAIKLLDEGAT
                     VPFIARYRKEATGNLDDTQLRTLQERLLYLRELEERRQVILSSIEQQGKLTEQLRNTI
                     EQTTTKQLLEDIYLPYKPKRRTRAQIARLAGLEPLAEALLTNPLLNPELEAEKYIRIE
                     PATDQSEAINVPTAKAALEGARDILVEKFSETAELLATMRAKLWNEGILSTTVIPGKE
                     QAEEEKFRDYYAYSEPIRTIPSHRALALFRGQALDVLRIDLKLADTQEELDFHPCAAL
                     IAKQFHIDNKNRPADKWLLEVCQWTWRVKLHTQVCVELFMQLREAAETEAIRIFSKNL
                     QELLLAAPAGAKVVLGIDPGFRTGCKIAVVDKTGKLVETTTIYPHQPQNCWKESLLTI
                     TQLVTQHKVELIAIGNGTASRETDKLTDEVIKFIEASSSHLRLAKIVISEAGASIYSA
                     SAFAAAEFPDLDVSLRGAVSIARRLQDPLAELVKIEPKSIGVGQYQHDINQRALTRAL
                     DATVEDCVNAVGVDINTASAPLLARVSGLNRTLAQNIVQYRDTTGPFINRQAILSVPR
                     LGNKTFEQAAGFLRITDGNNPLDRSAVHPEAYPVVERILNKLKKGISEVMKQPEALKG
                     LSPEEFTDETFGLLTIRDILSELEKPGRDPRPEFRTATFQEGIETLADLKVGMLLEGV
                     VTNVAAFGAFIDIGVHQDGLVHVSALANHFVKDPHQIVKPGQIVKVKVLAVDIPRQRI
                     SLTMRLDDPVDLHDQGKTQSSQSTSKPKPQRSNAKPEKPINTQPASALAIALSRMKTK
                     PEH"
     gene            11667..13145
                     /locus_tag="PKZ49_02100"
     CDS             11667..13145
                     /locus_tag="PKZ49_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme peroxidase family protein"
                     /protein_id="HND35383.1"
                     /translation="MLHGNVYRSDSMPPSSRFYDQGKFGRMFPNLPPFSLDSPSLRTA
                     LMKIGERRGIMDAQDDVDKDPSELILNPALRVNNPDNPDMSAGMTFLGQFLDHDMTLD
                     ITSSLEQQVDPEMIQNFRTPTFALDSIYGTGPSGSPYLFDQTIDHGFTTLLLEPITGS
                     ETKSRDGSLKYDLPRNSQNTPLIGDPRNDENLVLSQMQVAFIKFHNNLVIRLKAEGLT
                     NPVEIFLEAQRLTRWHYQWIILHEFLPKTIGQALVDDILQNGRKFYQWNNAPYIPVEF
                     SVAAYRFGHSQVRPSYRVNFGTNDANQIFALFFNDNLTNPVTIDMRGGSRAPERFIDW
                     QTFFDFGDNNARNNKRIDTKISTVMFDLPGMPANEPQSLAQRNLLRHLTFKVPSGQRV
                     AAAMGIPALLENDLADLKPFNLHNRTPLWFYVLREADVKADAKRLGPVGGRIVGEVMI
                     GLLQGDNTSYLSQDPDWTPHLGSNGNFTMVDLLKFAGVVTTL"
     gene            complement(13205..14315)
                     /gene="prfB"
                     /locus_tag="PKZ49_02105"
     CDS             complement(join(13205..14233,14235..14315))
                     /gene="prfB"
                     /locus_tag="PKZ49_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964773.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HND35384.1"
                     /translation="MEAEQLNTLRNQLVELNDRQKSLRRYLDFDNKKLRLIEVSRHAE
                     DPAVWNDAQRAQEVGREKKSLENVVLLLESLEKQLQDADELLQIAIEENDATAFQSIV
                     ADVAQLEKTIADMEFRRMFSNPMDPCNCFVDIQSGSGGTEAQDWAQMLERMYLRYCER
                     HGFEVELLEESAGDTAGIKSATLKVSGEYAYGYLRTETGVHRLVRKSPFDSGNRRHTS
                     FASVFVYPEVDDSIDIEINPAELRIDTFRASGAGGQHINKTDSAVRITHLPTNIVVQC
                     QSGRSQHRNKADAMAMLKSRLYEAELRKRNETKQAVEDAKTDIAWGYQIRSYVLDQSR
                     IKDLRTNVEIGNTQAVLDGDLDDFIQASLKQGVAS"
     gene            14518..15477
                     /locus_tag="PKZ49_02110"
     CDS             14518..15477
                     /locus_tag="PKZ49_02110"
                     /inference="COORDINATES: protein motif:HMM:TIGR03508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DmsE family decaheme c-type cytochrome"
                     /protein_id="HND35385.1"
                     /translation="MSRQIKKGLLHLLPMLVLALALGISPNSEAKSASEDADDESERG
                     IVLRKDRECTDCHDESSEYPILAIGKTKHGTTADSRTPDSCKSCHGESNDHLDGETTM
                     PMPSITFGKNSKNPIDERNQICLNCHQGGKRMHWLSSTHANRDTACTACHQIHAAQDK
                     VRDRIAQAEVCFACHKEQRAQINRPSRHPIREGKVVCSDCHNPHGSAGPSKMIRDSVN
                     ETCFSCHMEKRGPFIYNHPPVQENCAICHNPHGTTAPNLLKVRSPFLCEQCHEPNTHQ
                     SSPATLTGYYRRNTLARGCMNCHTQIHGSNNPENIRNEEVFQR"
     gene            15498..17606
                     /locus_tag="PKZ49_02115"
     CDS             15498..17606
                     /locus_tag="PKZ49_02115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtrB/PioB family decaheme-associated outer
                     membrane protein"
                     /protein_id="HND35386.1"
                     /translation="MKQRNIFHLSTIAAALIITMPLETLGDDTIKQMTKPQSTIQFGA
                     GYLSDDNARFGQFNGLRDEGAYGIFNVDILRRNDDSGTWFKIIGRNLGLQNREIRLEH
                     SKQGNWGYSIEFNQTPRYEPFTAITAVEGIGSSQLEVPTTPTTGNPTQLKTEREAISV
                     GINKILPGNFELNLHFRNEEKDGSRIFGRGNRLGAPSPTAVGGFEFTPEPINFTTRQF
                     GATLDYTGKDLQVTGGYHGSIFLNKNSALHINGGSDVGGAYAPSNFSPIALPPDNQAH
                     QAFLSGGYSFTPTTRGTLKASYTRATQSDSFFPTQLSTPGIGSNLGGQVDTALVQSSL
                     TARPLPKLSLLANARFEDRDDKTPVFLYNPMALELDFNGNRWSGLNQPRSFRTWNSKF
                     ESSYVLPMNFRIIGGIDYEHWDRTGLPATTGHRSTTDEISYRAELRRTLAETVTGTIS
                     YIHSDRFGSHFLTNRTATGDPFFNFIAPINLADRKRDKVRLSINWEPIDPLSVQVMID
                     ESWDNYGHRAGSDLGVQKGSARNYSIDAVYTFSEAWQATGWFSRNETFLDQASLTPAP
                     PRQLQERWTVGLENISTAFGAGLNGKPTDKIDVGANLEYSDITDKFNQSVITNAQTYT
                     APNINFQLARFRLFARYAIQRNYGIRLDYILDHFKTNEWTWNSNIPAQNWVYTDGTQL
                     TQDRSQLINFVGFSAYYSWQ"
     gene            complement(17666..18346)
                     /locus_tag="PKZ49_02120"
     CDS             complement(17666..18346)
                     /locus_tag="PKZ49_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HND35387.1"
                     /translation="MSTHFSNPLATIELTTGSNPTHSIIWMHGLGADGNDFVPIIEEL
                     DLPKTIAIRFIFPHAPKQPVTVNGGFIMRAWYDIRSADLDHAEDEVGLKRSEQLIMQL
                     IEKEKSRGIASENIVLAGFSQGGVMSLFVGLRYAEKLGGVMVLSGYLPLAHQFESSAH
                     LVNHTTPIFIGHGREDMIVPMQLANKAKQQLIVKGYLVEWHEYNMAHSVCNDELADIS
                     KWLRRVLL"
     gene            18430..19527
                     /locus_tag="PKZ49_02125"
     CDS             18430..19527
                     /locus_tag="PKZ49_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HND35388.1"
                     /translation="MKRREFIKWVGMGATLLPTSTTILAQSLIPNQWRGYRLTYQVTL
                     PPQGKQAHLWLPLPDSIDTPRQLIQGSVWNGTTKAGKLFTLPKTDTNLFHAEWQGFSE
                     RTVTVSSIVKTADRKVDLRFYTPSAKSSYPENIKRFLQPTQHIVIDGAIRKLADSIIK
                     KEEIRSPLNKARAIYDWVIDHSQFDKNIRGVGKGDIKQITINDPMLGKSADISALFVA
                     LARAVGIPARQQFGLRIDESAIHHDLGKLGDITTAQHCRAGFFLAELGWIPVDPAEVC
                     EVIALENLAEQPEKIAALKERLFGSWEMNWIALNDDEDVSLSHGKLTTKLPFLIYPHA
                     LIDGEPIDSLNPNEFSYKITSALLVGTGGKF"
     gene            complement(19658..19981)
                     /locus_tag="PKZ49_02130"
     CDS             complement(19658..19981)
                     /locus_tag="PKZ49_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3470 domain-containing protein"
                     /protein_id="HND35389.1"
                     /translation="MTYVVTESCIKCKYTDCVDVCPVDCFREGPNFLVIDPDECIDCT
                     LCVAECPVEAIYAEDDVPDNQRDFIALNGELSKLWKPIIEKKDALADADDWAKVTDKV
                     DHLER"
     gene            complement(20082..21284)
                     /locus_tag="PKZ49_02135"
     CDS             complement(20082..21284)
                     /locus_tag="PKZ49_02135"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HND35390.1"
                     /translation="MRNNKTKKVFLKAGKEKSLLRRHPWVFSGAVDRTENLPTLSDTV
                     HIFSAQGEFLAQAAYHPQAQIAARVWSWEQELSIDADFIHQRIAQAVAFRRQMGFAKL
                     STGMRIIHGESDGLPGLVVDQYDRVVVMQISSVGIERWREACIKSLRDEFDPICIYER
                     SDTEVRVLESLPERCGVVFGDLPANITIKEHGLSFQIDVSSGQKTGFYLDQRDNRALV
                     GALAADKDVLNCFCYSGGFTVYAKQAGARSVLSIDASQSALTLAMQNVALNGFATDES
                     EWVCADVFQALRKLRDQNRSFDLIILDPPKFAPTASFAEKAARGYKDINLLGFKLLRP
                     GGILASYSCSSGISSDLFQKIIAGAALDAGVEAHIIAYQNAAADHPVLLSFPEGAYLK
                     GLILKKRL"
     gene            21534..21609
                     /locus_tag="PKZ49_02140"
     tRNA            21534..21609
                     /locus_tag="PKZ49_02140"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21567..21569,aa:Asn,seq:gtt)
BASE COUNT         6497 a         5058 c         4224 g         5830 t
ORIGIN      
        1 tatcccatta tttttgatat tcgtaaaaca aaaacaaaat aaatcactct ggattgcctc
       61 attctttcat cgttctgccc tcccgagata aaggttcctg taagtccaat gcgatccagg
      121 gagattaata tcaaaaacca aggacaattt accgcctgcg gctagggtaa tcctgatgta
      181 ataactaatt tgggatcagc tgattgatta gcttgcaaaa tgcgggcatt tatgtacggt
      241 atttaataaa tcggtgttct taagaagcca ttcaattgac cgttagctat ttcaatcatt
      301 tttttacggt ctctacatac tatatacatc taaagggggt atttgattat gtcaattcag
      361 ggatttacgc cggcacaaat tgccgctttt acaactagtc agatccagag tctgacaacc
      421 gacgatttca agaaactttc taccgcacaa atcaatgcac tcaccagtga acaaattggc
      481 gctattgaga cagaagatct gagcgtactc agcgctactc agatcaagag ttttgatgct
      541 caagaatttg cggatggcat gctaacttac cagatcgaag cattaagcca aactcatatt
      601 gccgcactga gtaccgatca attgtcggta ctgacgactg atcaaatcca agcaatagaa
      661 actcgtgact taatcaaact cagcgcagcc caacttaatg cactcagtac tgaacaattt
      721 aacaacctaa gttccgatca aatacagtcg ctgacggcca ctcagatcaa aggactctcg
      781 actgatcaat tgtccacatt gacgactgat aatgttcaac tactcactgg ggcacagatc
      841 gcctcactat ccatcacgca attaaacagc tttgactcgg accaaattgc agccattgat
      901 gttgccgatg tagctgcctt taccgcaacc caagtcaagg gaatggatag cagtcaaatt
      961 gctgccttaa ccaccagtca aatcactcag ttgagcaata accaacttgc agcattgaca
     1021 accactcaga tcgccaatat tgaaacggat gatcttgctg ctatgacaag tagtcaaatt
     1081 actgcattct cagctaaaca aattgtcgcc tttacaagcg atcagctcaa caatatgaca
     1141 tctgatcaga ttcaggcact aactgctact cagatcaaag gccttactac tgaccaagta
     1201 tcaagtttaa caagcgataa tatccagtta ttgaccagcg ctcaggtcgc tgcgctgtca
     1261 acagctcagt tgaacagcct tgacagcgat caaatagccg cgctcgacac cggagatgtg
     1321 gccgcactga ctgccaatca aatcaaaggt cttggcacga cacaaatcca agacttaact
     1381 tctgatcaat tgcaggcatt gactgccaat cagatcaagg gacttacgac tgatcaaatc
     1441 gccaatattg aaacggatga tcttgctgct atgacaagta gtcaaattgc tgcattctca
     1501 gccaaacaga tcgccgcttt cacaagcgat cagctcaaca atatgacatc tgatcaaatt
     1561 caggtactaa ctgctgctca gatcaaaggc cttactactg accaaatgtc aagtttaaca
     1621 agcgataata tccagttatt gaccagcact caggtcgctg cattgtccac aactcagttg
     1681 aacagccttg acagcgatca aatagccgcg ctcgacaccg aagatgtggc cgcactaact
     1741 gcaacgcaga tcaagggact tggtacgaca caaatccaag acttaacttc tgatcaactg
     1801 caggcattga ctgccaatca gatcaaggga cttacgactg atcaaatcgc caatattgaa
     1861 acggatgatc ttgctgctat gacaagtagt caaattgctg cattctcagc caaacagatc
     1921 gccgctttca caagcgatca gctcaacaat atgacatctg atcaaattca ggtactaact
     1981 gctgctcaga tcaaaggcct tactactgac caaatgtcaa gtttaacaag cgataatatc
     2041 cagttattga ccagcactca ggtcgctgca ttgtccacaa ctcagttgaa cagccttgac
     2101 agcgatcaaa tagccgcgct cgataccgaa gatgtggccg cactgactgc aacgcagatc
     2161 aagggacttg gtactgatca ggttgtggca ctaactacca gccaagcagc tgcattgcaa
     2221 tatgcacaaa ttgccgcact cagtgtgaat caaattgcag tctttgaaac tgatgattta
     2281 gcgcaactga gcagtgacaa tatcaaagcg ttgtcatcct tgcagatcag ggcactaacc
     2341 actgatcaaa ttgatagttt atcgaccgag caaatccagg gactgaatgc cgcgcagatt
     2401 gcagcaatga catcggagca aatcagcaca ctcggaacag aagatattca ggcactaacc
     2461 acagcacaaa tcagcgcttt aacgagtgag cagatttctg gtttaaccac ggaacatata
     2521 tccaacctag aaagcgagga tttgcaagca ttgtctgcta tccagattaa actgtttaat
     2581 acagaccaaa tcaccaatgg tctttcaaca acgcaaatac agcaattgac tacaacacaa
     2641 gttgctgcgt tgacaacaga tcatatcccg aatctaagta cagatcagat cgatgcgtta
     2701 tctaccgatc aggtaagagc attgacatca gatcaaatcg cagtgcttac caccgatcag
     2761 attccttatc tgcacttcta atcggaaaca aatccataac gctgcacaac ttatcccacg
     2821 gtatagtatc tcgtgatcta taccgtggtc tttctctggg agagcattga atgtacgatc
     2881 agccgcattc cgactatacc gcctggattc agggtatctg gcaaagagtt gttaaatttt
     2941 cagatcttat tacttacgcc aatcaaaaaa acttcccgtc gagtctagcc gctgtactat
     3001 atcaaacttg gctcaagcgc aatcacacta cagacgatgt attcgcttgg ttcaatctgg
     3061 gtgtcagcct attttcagaa aaagatttta ttggtgcccg agaagcttat gaagcagcac
     3121 tagccattaa acccaatttt attgcagcac gtttcaacct tggattgatt tacgaacggt
     3181 tgaataatac tcaagcagcg cttgctcaat ggaaacaagt agaagaaact gccaattcag
     3241 ccgtacctga cgatcttgct attctcgttt tagcactgaa tagcactggt cgactacaag
     3301 aaacgctcaa acaataccaa cttgcatgtg aagctctaga aaaaagccta ttactaaaac
     3361 caaaccagtc ggatgttatt catcatttga tttttatgcg tcaaaaacaa tgtcgctggc
     3421 caatttatga gtcagttggt gctaccggag aaagtgcgtt gcgaaaagcg acttcagcgc
     3481 ttgctatgct gaatatttcc gatgacccct atactcagct tgaaggcgca ctgaactata
     3541 gccgagcaaa gatacccact gatctaccta ggcttgctcc acccaatggc taccagcata
     3601 aaaaaattcg tatcgcctat tgctctggtg atttctgcac acaccccgtt gctatgctga
     3661 ctgttgaatt atttgagcaa catgacaaag ataaatttga gatttacgct ttctgctggt
     3721 cacccaatga tggttcgaac ttacgccagc gcattattca gggagtagat cattacatcc
     3781 caattcacga gctcaatgaa gcagattccg cccgactgat ccgcgagtat gaaattgaca
     3841 ttctggttga tctacacggt caaacaagca acgctagaat ccaaatgcta gcacatcgcc
     3901 ctgcacccat ccaaatcacc tacctaggac tccccgcgac aacggggcta ccctgtatag
     3961 actatgtaat tgcagatcgt ttcctaattc cccaagagta cgcaaatttt tattcagaga
     4021 agcccctcta catgccggat atataccaag tcagtgatcg caagcgtgaa atcagttccg
     4081 ctcctactag ggaatcttgc ggactaccca aaaataaatt tgtattctgc tccttcaata
     4141 acaatcacaa atataccctg gaagtattta ctacatggat gaatatttta cgacgtgttc
     4201 ctgaaagcgt tttatggtta cttgctgata atccttgggc aaaatccaat ctcaagaagc
     4261 aagcagaatt gcaaggtatc gacccaactc gattgatttt tgcaaaacgc accctgccag
     4321 ctgactatct agcacgctat catgttgccg atctatttct agatacattt cctttcaacg
     4381 ctggaactac agccaatgat tccttgtggg ctgggcttcc tgttcttacg ttatgtggtc
     4441 gctcatttgc ctcccgaatg gccggtgcct tgttgacaac cgcagattta cctgagctca
     4501 tcacttacga tttgaaatcc tacgaagata aggctgtcga gctagcacag aatcctaaag
     4561 tagtaaaaaa attacgcaag aaactagctg atgccaaaat aaatagccca ctatttgaca
     4621 ctacccggtt cgccagaaat ctggaaacac attacactaa tctggttgct gcctttaatt
     4681 atcaggcatc ttcagtccag tctgccaaac aggctgcttc agtttccgaa caaccccaga
     4741 agttaaaaca acttaccgat attgtcacca aagcagaaaa acttcaggcc caagacgata
     4801 taatcagtgc aatccaaact tatcatcagt ggatcaaata ttcttgttca caggataaat
     4861 ggatagcctg gtttaatcta ggtgcgctgc tcagtagtag cggcgatctc actggagcag
     4921 aacaggcttt tcaagtagta cttcagcagc agcccggttt tattcaggca agaaaagcgc
     4981 tggaggtatt acatagttgc atgccgaaaa gtaagcaact atgtagcgag aatgataaaa
     5041 atgcactaga tttctggaag tgtgtaacct ctgaaactaa aaagccgcta gtagcacatt
     5101 cttagattga tgtgtctaca cttttctgga cacaaagtta agcaacattt tgctgcaact
     5161 cgataaagaa ccccgccaca agtagcgggg tatttggtct caaaattact aaccgtactc
     5221 gcaaaataac catcagccca aaattcttcg ccccatagtt tcttcttaac gagggtaacg
     5281 cttgaaaatt tctcgtgcag ttatgctctt aataagctag tagcacttat gtcggcactg
     5341 actataccaa gaaatcaaga tgatccttat cagtaccaat ctctaaaaac ttcacttgat
     5401 agcgctctgc caattcaagg caaatacctt tgaaaacaac attaacttgc tcatcaaaaa
     5461 ctacacctct gtatcttgcc agaaatacta agtagtacag taaaacttga cattatgact
     5521 cttatgtata tattcgctca tccacaaatt ctacgctgca agcggcagga aatatcatcc
     5581 aaagaaattc aactgagcaa cggtagatca aaaagagagc ttacatagct gtcaggtacg
     5641 cccttaaacg ctaacgcaac aatttcaaga tttcttgttg tagtatatat tttatagaaa
     5701 cctgcatttt gtagcgcttt ctcaagtgat ttctgagtaa aacctgtttt gtgactgaag
     5761 aaatcttgcc cactacgctc gatttcgaca ctatagccat ataaaacatc tgacaccata
     5821 atcggcccag cggaagattg atacaaaaaa tcatcaagat ctagattccg ctccatcgta
     5881 atgcgcataa cttcttgaat atctggcacc cggatatgga caaatccatt ctcgttcaga
     5941 acatgcaaga aaccgttcag aaccttcttg acgtcatgcc gataatagtg ctcaaggtta
     6001 tgtgaacaat agattgcatc aaactggtca gcatccagag ttgccaaatt ccgtgcatcg
     6061 cacacaaaat caggctcgcc tcgcgggtca atatcgagca ggagatgttc aaactctaca
     6121 tactggggcg gcagtgggat cgatttattg tttcccccta cgttcaggac ttttttcatt
     6181 ttcagcttcc ttgtatatat aacgtcaaag agctgttaat ttaactaata cagataatat
     6241 tttcttttgg aactatgcga gaaaaaccgg ttatttctag cttctctcgc tatctaacac
     6301 cataaactga cagtacacag acaataaaaa atgcagtgta ctgcgttcag gcactttgca
     6361 aaaagctgaa tttatatttg caagcgaaaa cgtaggtcaa aataacaatt tgatggcaaa
     6421 catcataaag aagcggcgcg tgattttaaa tgatcaaggt ggggggaaga atcacttaaa
     6481 cgcgccgcta ggagggagac acaaaaatct aacttgttgc gtaaaaatta ataatttcta
     6541 ttaaccgagt aataaaaaag agattccttt tagaaagcta agcttcgctg gcttgtccat
     6601 cgctcttgtt tatagcgctg ccacgccagt tctttcttca taaccgaatc gatcgttttt
     6661 agtagcccat caatattgac tggcttatgt gtttctacca atacgcctgt caatggagta
     6721 tcccaatcta gtttcccgcg ttcgtatagt gaccaaattt ccgatccaaa atctgtctta
     6781 gcgagttcag gggaaaaatg actaaaatat tttgtgatat tgtcaatatc tcgcttcaaa
     6841 agccaacgcg cattattatt ctctactgca taaatggctt gcggaaaatc gatgattaca
     6901 ggtccttggg aaccaagcaa tacattgtat tcggataaat caccatgaat catgccacga
     6961 catagcatac gaaccacctg attaatcagt aatgtgtgat aggatttcgc ttgttccgtc
     7021 gttaacctga tatcgttcaa acgaggtgcc acattacctt gggaatccgt gataagttcc
     7081 attaaaagca caccctcgaa gaaattaatg taccttggaa cttttacacc tgctgcactc
     7141 agtcggcata atgcattaat ttcagtattc tgccaggcct cttctcgagc tacttttcca
     7201 aagcagcttc gtttctgaat ctcacgtgta cgacgaaaat ttttcaagta acggccttca
     7261 gtataatttg cgctatgccg aaaattgcgt cgatgtgttt ctttgtatac tttggcacat
     7321 cgcagctcac ttccacattg cacaacataa accaacgctt ctttaccact catgagttgg
     7381 caaatcactg aatcaactaa accttcttct atcaatgatt cgatttgttt aggggttttc
     7441 atttttgtct catctctata ttagaaaaca attgattaat tgctcaattt tgttgaaaac
     7501 gtgctgattt actgcgatgc caggatgcat cttccatctg acttaaatgt gtcgttgtat
     7561 tctgcttgat cgatttcgtt tgtggcttgt tttcaccgaa ctcactggtc ccaattgtaa
     7621 ctttgcttga gtgagacgaa acaccatgct gattatgttt gagcgtcata cggatctcct
     7681 agttagtaag gtcattcatt actgcaatga tttactcatg gctgcattgt aaagttaaaa
     7741 aaattacttt ggaatgtggc atattgccgc gcgacaattt gtcgcagcgc atgattgaag
     7801 cagaccatta gtcgcaaatc gattaaaata gcgccttaca actttcataa acaatagaat
     7861 agagtcattc atcgaatatg gattttgacg aagagaactt atttccacat caagacgcat
     7921 tagcgcagct acatgaaaac cgtcctctat ccgagaagct caatttttta catcagctaa
     7981 tccgtgaaga ctttccgttt attgatcgtt tggcgattgc gctgcttgac gagaaaacga
     8041 gaacactcaa aacctatacc cacagtaccg acgacggcaa tagcccgatt actgcctatg
     8101 aagcttctct cgattccgcc ccatccctgc tggaaatcat taagcgccgt cgtcctcgac
     8161 tcgttcagga tttagcaatt tttaataaag gtgcccacca gcacacaaaa ttgatcgcag
     8221 ccaaaggcta taaggcgagc tataccatgc cactgtatca aagtggtgta ttcgtaggat
     8281 tcttattttt taactcttat gaatctcatc cattcttacc accgattctg cgtcaaatcg
     8341 acatttatgg tcatttgatt gcgctaatgg ttatcagtga attaacggcc atccgcacat
     8401 tactctccgc agtaaaagca gcgcgtagta tgacgcacta tcgcgatgtg gaaaccggct
     8461 cccacgttga tcgtacagcg cattatgcgc gtttgattgc aaaagaactg gcacaacact
     8521 atcagttgcg tgatgagcag atcgaacata tttttttgtt ctctccgtta cacgatatcg
     8581 ggaaaattgg tattccagat agcatcttgc gtaaaccggg taaattagat gatcaagaat
     8641 ttgccgttat gaaaacccac cctggcaaag gacgagaaat tattgatagt atcttgaaag
     8701 attttagttt aggtacattt ggacatgttg aaattttgcg caatatcgca gaataccatc
     8761 atgaagcgat tgacggcagc ggttatccta accaacttca aggtaatgag attcctatcg
     8821 aagccagaat cagtgccgtt gccgatgtat ttgatgcctt gacttccagt cgaacgtata
     8881 aagccgcttg gtcgaacgaa gaagcatttg ccatgttgca gcgcttgtcc gaaaacaaac
     8941 ttgataaaga ttgtgtggaa gcgcttatca aaaataaaga taagatatta gagatacaac
     9001 aactctttcg tgattcagaa gataaataac ttgtcacaat caaaatcacg atcccacaat
     9061 ttgtccttga tacccttcaa aaatgagcac acccatccaa acgaatacgt taaattctac
     9121 gcaagcaaaa attattagtt tgattgcaac tgaattaact gcaaaaacac cccaagtctc
     9181 cgcagccatc aaactactcg atgagggagc cacggttccc tttattgctc gctatcgcaa
     9241 agaagccaca ggtaatctcg atgatacaca actgcgcacg ttgcaagaac gtttgcttta
     9301 tttacgagag ctcgaagaac gaaggcaagt cattctatcc tcaatcgagc aacagggaaa
     9361 actaacggaa caactgcgca acacgattga gcaaaccacc accaagcaat tactcgaaga
     9421 tatttattta ccttataaac ctaaacgccg tactcgcgca cagattgcgc gcttagctgg
     9481 attagaaccg ttagctgaag cgctattaac aaatccttta ctcaatcctg aattagaagc
     9541 tgagaaatat attcgaatcg agccagcaac cgatcagagt gaagcgatca atgttccaac
     9601 tgccaaggct gcactagaag gtgcgcgtga tatcttagtg gaaaaattct cagagacggc
     9661 cgaattactt gccacaatgc gcgcgaagct atggaatgaa ggcatactct cgacaactgt
     9721 aattcccggt aaggagcaag ctgaagaaga aaaatttcgt gattattacg cttattccga
     9781 gccgattcga acgatcccct ctcatcgggc attagcactc tttcgagggc aagcactcga
     9841 tgtgcttcga atcgatttaa aattagcaga tacccaggaa gagcttgatt ttcatccctg
     9901 cgcagcgctc atcgctaaac aatttcatat tgataataaa aatcggcctg cagacaaatg
     9961 gctgctagaa gtatgtcagt ggacctggcg agttaaatta catactcaag tctgtgttga
    10021 gctgttcatg caattaaggg aagctgctga gactgaagca atcaggattt ttagcaaaaa
    10081 cttacaggaa ctactattag cagcacctgc tggcgctaaa gtcgtactag gcattgaccc
    10141 aggattcaga accggttgta aaattgcagt tgttgataaa acaggaaaat tagttgaaac
    10201 gactacgatt tatccccacc aaccacaaaa ttgctggaaa gaatcgctat taacgattac
    10261 acagttagtt acccaacata aggttgaatt gattgccatt ggcaatggta ctgcgagcag
    10321 agaaacagat aagttgacgg atgaagttat taaatttatc gaagcaagtt cttctcatct
    10381 tcggctcgct aaaattgtga ttagcgaagc aggagcttct atttactcag cttcagcctt
    10441 tgctgccgca gaatttcctg atcttgatgt tagtctgcgg ggagccgtgt cgattgcacg
    10501 tcgcctacaa gaccctcttg ctgaactcgt taaaatcgaa cctaaatcca ttggtgttgg
    10561 gcaatatcaa catgatatca atcaacgcgc actaacacgt gcacttgatg caacagtcga
    10621 ggattgcgtt aatgcagtgg gtgttgatat taacacggct tctgcaccgt tattggcgcg
    10681 tgtatcagga ctgaatcgca cgttagcaca gaatatcgtg caatatcgtg atacgacagg
    10741 cccttttatc aatcggcaag cgatattgag tgttccaagg ctcggaaata aaacattcga
    10801 gcaagcggct ggttttctac gtattaccga tggtaacaat ccgcttgacc gctctgctgt
    10861 tcatccagaa gcgtatcctg tcgttgagcg aattttaaat aagctcaaaa aagggattag
    10921 tgaggtcatg aaacaacccg aggcgctaaa aggactttcc cctgaagaat ttacggatga
    10981 aacctttggt ctgctaacta tccgcgatat tttgtctgaa ctcgaaaagc caggacgtga
    11041 cccccgtcct gaatttagaa cggctacctt tcaagaagga attgaaacac ttgctgatct
    11101 aaaagtagga atgcttctgg aaggtgtcgt aaccaatgtt gctgcttttg gggctttcat
    11161 tgatatcggt gttcatcaag acggattagt tcatgtatcc gccctggcca atcattttgt
    11221 caaagaccct catcaaattg tcaaacccgg tcagattgtc aaagtaaaag tattggctgt
    11281 ggatattccc cgccaacgga ttagcttgac aatgcgatta gacgacccag tcgatcttca
    11341 tgatcaaggt aaaactcagt catcccaatc gacttcaaaa cccaaaccgc agcgttctaa
    11401 tgctaagccg gaaaaaccca tcaatactca acctgcaagt gctttagcaa tcgctttatc
    11461 tcgaatgaaa acaaaacctg aacattgatc tctgccatcc ctccacacac gaccattcga
    11521 tttaaaccga gaaggctact catcgctatg cgtgtgagaa atcacacatt catataagcg
    11581 aacaaaacct atcatgcatc tgttgatgga aaaattgaat gtttccttca tacagacaac
    11641 ccaattagct tgtataggag aataaaatgt tacatggcaa tgtttaccgt tcagatagta
    11701 tgccgccaag ttccagattt tacgatcaag gaaaatttgg aagaatgttt ccaaacctgc
    11761 cacctttttc attagattca ccgagcttgc gtaccgcgct catgaaaatc ggtgaacgac
    11821 gtggcatcat ggatgcacaa gatgatgtag ataaagatcc ttcagaactc attcttaatc
    11881 ctgcactgcg ggttaataac cctgataacc ctgatatgag tgctgggatg actttccttg
    11941 gtcaatttct tgaccatgac atgacgctcg atattacttc tagcctagag cagcaagtcg
    12001 atcctgaaat gattcagaat ttccgaacac caacctttgc attagatagt atctatggta
    12061 caggcccatc tggctcacct tatctatttg atcagacaat cgatcatgga tttactactt
    12121 tgttgctaga accaattacc ggctctgaaa ccaaatcccg tgatggatct ttgaaatatg
    12181 atctaccccg caatagtcaa aatacaccgc tgattggcga tcctcgcaat gatgaaaatc
    12241 tagttttgtc ccaaatgcaa gtcgcgttca tcaaatttca taataacttg gtaatcagac
    12301 tcaaagcaga ggggctaacc aatccggttg aaatctttct tgaagcacag cgtttgacgc
    12361 gttggcatta tcaatggatt attttgcatg agttcttacc caaaacaatt ggtcaggcct
    12421 tggtagatga tatattgcaa aatggtcgga aattttacca atggaacaac gccccttata
    12481 ttccagtcga gttttcagtt gctgcctatc gttttgggca ttcacaagta agacccagct
    12541 atcgggtcaa ttttggtaca aatgacgcta atcagatttt tgccctattt ttcaatgata
    12601 acttaacaaa cccggtcacc attgacatgc gtggcggttc aagagcaccc gaacgtttta
    12661 tcgattggca aacatttttt gattttggtg ataacaacgc acgcaataat aagcgtattg
    12721 acacaaaaat ctctacagtt atgttcgatc ttccaggtat gcctgcaaat gagcctcaat
    12781 cccttgcgca acgcaatcta ctgcgccact tgacattcaa agttccgtct ggccaacgcg
    12841 ttgcagctgc catgggaatt cctgcactac ttgaaaacga tctggctgat ctaaaaccat
    12901 ttaatctcca taatcgcaca cccttgtggt tctatgtatt acgtgaggct gatgtgaaag
    12961 ctgatgcaaa acggttaggc ccagttggcg gccgtattgt cggcgaagtc atgattgggc
    13021 ttttacaggg agacaatact tcctatcttt ctcaagaccc cgattggaca cctcatttag
    13081 gttcaaatgg taactttacg atggtagatt tactcaaatt tgcgggtgtt gtcacaacac
    13141 tataaatatc tcgcatttca agttgggttg atcatcaacc caactttttc taatcacacc
    13201 gatctcaaga agcaacccct tgttttaagc ttgcctggat gaaatcatcc aaatctccat
    13261 ccagcactgc ttgggtatta ccaatttcga cattggtacg tagatctttg atacgcgact
    13321 gatcaagcac ataggaacgt atctgataac cccacgcgat atctgttttt gcatcttcaa
    13381 ccgcttgttt ggtttcattg cgcttgcgca attccgcttc ataaaggcgc gacttgagca
    13441 tcgccatcgc atcagcttta ttgcgatgct gcgagcgccc actctggcac tgaacgacga
    13501 tattagttgg aagatgggta atcctaaccg ctgaatcggt tttattgata tgttgaccgc
    13561 cagcaccgga tgcgcggaac gtatcgatcc gcagttctgc tggatttatc tcaatatcaa
    13621 tactgtcatc gacttctgga taaacaaaca cgctagcaaa ggatgtatga cgccggttac
    13681 cagagtcaaa cggagatttt ctgaccaaac gatggactcc cgtttcagtc cgtaaatagc
    13741 cataagcata ttcaccggat actttaagcg ttgcactttt tatacctgcc gtatccccag
    13801 ccgattcttc taataattcc acctcaaaac catggcgctc gcaatagcgc aaatacatcc
    13861 gttctagcat ttgcgcccaa tcttgcgcct ctgtccctcc ggaacccgat tggatatcta
    13921 caaaacaatt gcaaggatcc attggattgg aaaacattct ccggaattcc atatctgcaa
    13981 tggttttttc gagttgcgca acatcggcga cgatgctctg aaacgccgtt gcgtcgtttt
    14041 cctcgattgc aatttgaagg agttcatccg catcttgcag ttgtttttct aatgactcga
    14101 gtagtaacac tacattctca agcgatttct tttcacgccc tacttcttgg gctcgctgcg
    14161 catcattcca gacagctggg tcctccgcat gtctagagac ttcaatcaac cgtagcttct
    14221 tattatcgaa gtcaaagata cctccttaac gatttttggc gatcgttcag ctcgactaat
    14281 tggtttctaa gcgtattgag ttgttctgct tccatcatga ttgccaccca aaaaatacaa
    14341 aattatacag gtttggctaa cccctcgtta aacgatactg caattgaatt tatttctcag
    14401 atcgtgagat atcacacaga tcggaatagc cctccagtta gataattttt tcatcttttg
    14461 ttgaagcaag aaacggcttc aaccctaacc ttaactaact tagagaggct atccatcatg
    14521 tcaagacaaa taaaaaaagg cttattacac ctactaccga tgctagtgtt agcactagca
    14581 ctcgggatat cccccaattc tgaagcaaaa agcgccagtg aagatgctga tgacgaatcg
    14641 gaacgcggta ttgtcctcag gaaagacaga gaatgtactg actgccacga tgaaagcagt
    14701 gagtatccaa ttcttgctat tggtaagact aaacatggca caacagcgga tagcagaaca
    14761 ccagactcct gcaaaagctg tcatggtgaa agtaatgatc atttggatgg tgaaaccacg
    14821 atgcccatgc catcgattac gtttggaaaa aactccaaaa accctatcga tgaaagaaat
    14881 caaatctgtc taaattgtca ccaaggcggt aaacgcatgc attggttgag cagcacccat
    14941 gcaaaccgag atacggcttg caccgcctgt catcaaattc atgcagcgca agacaaagtt
    15001 cgtgaccgaa ttgctcaagc cgaagtatgt tttgcgtgcc ataaagaaca gcgagctcaa
    15061 atcaatcgac cttcaagaca cccgattcgt gaaggcaaag tcgtgtgttc cgattgccat
    15121 aaccctcatg gttcagcagg gccaagcaaa atgattagag atagtgtcaa cgaaacctgc
    15181 ttttcctgtc atatggaaaa acgaggaccc tttatttata accacccacc cgtccaggaa
    15241 aactgtgcca tttgccataa tcctcatgga acaacggcac cgaatttact gaaagtacgt
    15301 tctccttttc tctgcgaaca atgccacgaa ccgaacacgc atcaaagtag cccagccacc
    15361 cttacgggct attaccgtcg gaatactcta gccaggggat gcatgaattg ccacacgcaa
    15421 attcatggca gtaacaaccc tgagaatatt cgcaatgaag aagtcttcca gcgttaatcg
    15481 acaaggagga atgaatcatg aaacagcgaa atatttttca cctcagcacg attgctgcag
    15541 cgctaatcat cacaatgcca cttgaaacgt taggcgatga taccatcaag caaatgacta
    15601 aacctcaaag tacgattcaa tttggtgctg gttatttatc cgatgataac gcccgctttg
    15661 gacaatttaa tggtttgaga gatgaggggg cctatggcat tttcaatgtt gatatcttaa
    15721 gaagaaacga tgatagtggc acctggttca aaataatagg tcgtaatctg ggtttacaaa
    15781 atcgcgagat acgtctcgaa catagtaaac aaggaaactg gggatattct atcgagttta
    15841 atcaaacacc gcgttatgaa ccttttactg ccataacagc agtcgaaggt attggaagtt
    15901 cgcaactgga agtaccaacg actccaacga caggtaatcc tactcagctc aaaacggaaa
    15961 gggaagcgat cagcgtgggt atcaataaaa tcttacctgg aaattttgaa ctgaatcttc
    16021 atttccgtaa cgaagaaaaa gatggctcac gtatttttgg ccgtggaaac cgactgggag
    16081 cacctagccc cactgctgtc ggcggttttg agtttacacc tgagccaatt aattttacga
    16141 cgcgtcaatt tggggcgaca ttggattaca cgggtaaaga tttgcaagta acaggcggtt
    16201 accatggttc aattttcctg aataaaaatt ctgcacttca tatcaatggg ggcagtgatg
    16261 ttggtggagc ttatgcgccg agtaactttt cacccatagc gctcccccct gataatcagg
    16321 cacatcaagc ttttttatcc ggtggctata gctttactcc gacaacacgt ggaacactga
    16381 aggcctctta tacacgtgca acgcaatccg attcattttt tcctacccag ctctccacgc
    16441 ctggtatcgg aagtaatttg ggaggacagg tcgatacagc ccttgtacag agtagtttaa
    16501 ccgctcgccc tttacccaag ctttcattgc ttgcaaatgc acgatttgaa gaccgtgacg
    16561 ataagacacc tgttttcctt tacaacccta tggcactaga actggacttc aatggcaatc
    16621 gttggtcagg ccttaaccaa cctcgatcct ttagaacctg gaacagtaaa tttgagagca
    16681 gttatgtatt acccatgaat ttccgaatta ttggtggtat cgactatgag cattgggatc
    16741 gaacgggatt accggcaacc acaggccatc gcagcacaac tgacgaaata tcgtatcgag
    16801 ctgaattgcg acgcacactc gctgagaccg taacaggaac aatttcctac atccatagcg
    16861 atcgctttgg ttcacatttt ctgacaaatc gaacggctac tggtgatccc ttttttaatt
    16921 tcatcgcccc catcaatcta gccgatcgga aacgcgataa agttagatta tccatcaact
    16981 gggagccgat tgatcccctt tcggttcaag tcatgattga tgaatcatgg gataactacg
    17041 gtcatcgcgc gggctcagac ttaggggttc aaaaaggctc tgcaagaaat tattcaatcg
    17101 atgccgttta tactttctct gaagcctggc aagcaaccgg atggttttca cgaaatgaaa
    17161 catttctcga tcaagcctca ctgactcctg ccccacctcg tcagctccaa gaacgttgga
    17221 cagttggcct agagaacatt agcactgctt ttggtgctgg attaaatggc aaacccactg
    17281 acaaaatcga tgtcggtgct aatctcgagt atagcgatat tactgacaaa tttaaccagt
    17341 cggtgattac caatgcgcaa acttatacgg ccccgaacat caatttccag ctagctcgat
    17401 ttagattatt tgctagatac gcgattcaaa gaaactatgg aattcgcttg gattacatcc
    17461 tcgatcattt caaaaccaac gaatggactt ggaactcaaa tatccctgct cagaattggg
    17521 tctataccga tggaacgcag ctaacgcaag atcgcagtca attgattaat tttgtaggat
    17581 tctccgctta ttacagctgg caataatcat ccgtgcattc tgaaaacacg atctttaccc
    17641 tggtaaatgc tctcttcaga atgcactaca ataaaacgcg ccgcagccat ttactgatat
    17701 ccgcgagttc atcgttgcaa actgaatggg ccatgttgta ctcatgccat tccacaagat
    17761 agccttttac aatgagttgc tgcttggctt tattcgcaag ctgcatcggt acgatcatat
    17821 cctcgcggcc atgtccgata aatatcggtg tagtgtgatt aacaagatgg gcgctgctct
    17881 cgaattgatg ggcaagtggc aagtaaccgg acagtaccat cacaccaccc aatttctcag
    17941 catatcgcaa cccgacaaaa agcgacatta cccccccttg tgagaatccc gccagaacga
    18001 tattttccga tgcaattccg cgtgatttct ctttttcaat cagttgcata atcaattgct
    18061 cagatcgttt tagccctacc tcatcttcag catgatctaa atcagcgctg cgtatgtcgt
    18121 accaggcgcg catgataaac ccaccattga ccgtgaccgg ttgcttgggt gcatgcggaa
    18181 agataaatcg gatggctatt gttttaggga gatcgagctc ttcaataatg ggcacgaaat
    18241 cgttgccatc agcaccaagc ccatgcatcc agatgattga gtgagtcgga tttgagcctg
    18301 ttgttaattc gatcgttgct aacggatttg agaagtgagt agacatgaat tgaaggaact
    18361 cgccctggta aaatagtaga attcaaattt gaaaattcta tactaaactt aataacagag
    18421 gagacaggta tgaagcgcag agaatttatt aaatgggttg gtatgggagc tacgctactc
    18481 cccacaagta caaccattct tgcgcaatcg ttaattccga accaatggcg tgggtatcgg
    18541 ttaacctatc aagtaacctt accaccacaa ggaaagcaag cccatctctg gttaccgctc
    18601 cccgacagca ttgatactcc gcgtcaatta atacaaggaa gcgtttggaa tggcacaact
    18661 aaagctggaa aactttttac actacctaaa actgatacga atttattcca tgctgaatgg
    18721 caaggtttta gcgaacgcac cgtaaccgtc agtagcattg taaaaacagc cgatcgcaaa
    18781 gttgatttgc gattctatac accgtctgca aaaagtagct acccggagaa tatcaagcgt
    18841 ttcctccagc ctacccaaca catcgtaatc gatggagcca ttcgtaaatt agccgactcg
    18901 atcattaaga aagaagaaat tcgctctcca ctcaataaag cgagagcaat ttacgattgg
    18961 gtcattgatc actcacaatt tgataaaaat atccgcgggg taggaaaagg cgatatcaaa
    19021 caaatcacta taaacgaccc tatgcttggt aagagtgccg atatcagcgc gttgtttgta
    19081 gcgctagcgc gagcagtggg tattccagca cgtcagcaat ttggcttacg gattgatgag
    19141 tctgccattc atcatgattt aggaaaatta ggcgatatta caactgctca acactgccgt
    19201 gccggattct ttctcgcaga acttggatgg attccagttg atcctgcaga agtatgtgaa
    19261 gtcatcgcat tagaaaacct tgctgagcaa cccgaaaaaa tcgcagcact taaagaaaga
    19321 ttatttggct cttgggaaat gaactggatc gcactgaatg atgatgaaga tgtaagccta
    19381 agccatggaa aacttaccac taagctgcca tttcttattt atccacatgc tttgattgat
    19441 ggtgaaccaa ttgatagcct caatcccaat gaatttagct ataaaatcac ttcagcctta
    19501 ttagtgggta ccggcggtaa attctaaaaa gaaacgctat attgcatgaa tcaattacta
    19561 caatcttaaa acatcggtag taattgattc ataatagaaa ctggttacct accagtgatt
    19621 gacaattctt tacgtaaaat taatttaaga aataatctta acgctctaaa tgatcgactt
    19681 tatcggtcac ttttgcccaa tcatccgcat cagctagcgc atcttttttc tcgatgatcg
    19741 gtttccacaa cttagataat tcgccattta acgcaatgaa atcgcgctga ttatcgggca
    19801 catcatcttc agcataaatc gcttcaacag gacattccgc gacacaaagc gtgcaatcaa
    19861 tacactcatc tggatcgatg actaaaaaat taggtccttc acgaaaacag tcaaccgggc
    19921 atacatccac gcaatccgta tatttacatt taatacaact ttcagttact acataagtca
    19981 tgatgaaatt ccttgcaagg cactttttaa tatcagaacg tataatttta tcagaaaatc
    20041 gaagcggact tctaccctcc tacgctacga tcaaaacgta tttatagtct cttttttaaa
    20101 attaagcctt tgagataagc cccctcagga aaactaagca ataccggatg atcagctgct
    20161 gcattctggt aagcaatgat atgcgcttca actcctgcat ctagggctgc tcctgcgatg
    20221 attttttgaa ataaatcact actgatgcca ctggagcatg aataacttgc taagattcca
    20281 cctggtctaa gcaatttgaa ccccaacaaa ttaatatcct tatacccacg cgctgctttt
    20341 tcggcaaaac ttgctgtagg agcaaatttt ggggggtcta aaataattaa gtcaaagcta
    20401 cgattttgat ctcttaattt tcgtaatgct tgaaaaacat cagcacaaac ccattcactc
    20461 tcatcggtcg caaatccatt gagcgcaaca ttttgcatcg caagtgtcag cgccgactgc
    20521 gaggcatcga tcgaaagcac tgagcgtgcc cccgcttgct ttgcataaac tgtgaatccc
    20581 ccgctgtaac agaagcaatt gagcacatct ttatctgccg ctagtgcacc aaccaaggca
    20641 cggttatcgc gctgatctaa ataaaacccc gttttttgcc cactactgac atcaatctga
    20701 aaagacaaac catgctcttt aatggttatg ttagctggta aatcaccgaa tacaacacca
    20761 catcgttcag gcaacgactc aagtacccgc acttcagtat ctgaacgctc ataaatacaa
    20821 ataggatcaa attcgtcccg caagctttta atacaggcct cccgccagcg ttcgatacca
    20881 acgctgctga tttgcataac taccacccga tcatactgat caaccacaag cccaggcaac
    20941 ccatcagatt caccatgaat gatgcgcatc cctgtactta gtttagcaaa acccatttga
    21001 cggcggaaag caacggcttg agcaattctt tgatgaataa aatcagcatc gattgataat
    21061 tcttgctccc aagaccaaac ccgagcggca atttgtgcct gcggatgata agcagcctgc
    21121 gctagaaatt caccctgcgc agaaaaaata tgtacggtat cactcaatgt tggtaaattt
    21181 tccgtacggt caactgcacc cgaaaaaacc caaggatggc gacgtaataa tgatttctct
    21241 tttcctgctt taaggaatac ttttttagtt ttattgtttc tcatgcagtc tcttcaaaat
    21301 ttagcctttg cttcagcaga caagctaata actaggtgat catatcccga agcgacatat
    21361 ccttcccaaa ataatttgat catgcctctt ttttctttga tttgaatata actcattcaa
    21421 ttcacgaaaa aatatgtatt ttacatttag ataaagtctg tttcctgata agaacagcgc
    21481 acaacgaagt tgaccaatga atatcaaagc gttactataa gcatcgcttc cattccctga
    21541 tagctcagtc ggtagagcga cggactgtta atccgcaggt cccaggttcg agccctggtc
    21601 ggggagcca
//
LOCUS       DAOQKQ010000018        21541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_1054_length_21541_cov_5.775994, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000018 DAOQKQ010000000
VERSION     DAOQKQ010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 21541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21541
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1054_length_21541_cov_5.775994"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="PKZ49_02145"
     CDS             <1..497
                     /locus_tag="PKZ49_02145"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480501.1"
                     /note="catalyzes the formation of N-carbamoyl-L-aspartate
                     from (S)-dihydroorotate in pyrimidine biosynthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HND35391.1"
                     /translation="RSPNHIAATITAHHLLFNRNAIFTSGIRPHYYCLPILKREKHRQ
                     SLVAAAISGSPKFFLGTDSAPHSIANKESNCGCAGIYSAHAGIEFYVEAFDRAGALDK
                     LEAFASFHGADFYQLPRNEHQITLIKQDWIIPAQIRFGDEQLIPLMAGEKLSWKLSNH
                     FLAW"
     gene            694..1296
                     /gene="rpoE"
                     /locus_tag="PKZ49_02150"
     CDS             694..1296
                     /gene="rpoE"
                     /locus_tag="PKZ49_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HND35392.1"
                     /translation="MSDREIDQQLVERVQQGDKRAFDLLVVKYQRKLARLLSQLIRDP
                     VEVEDIAQETFIKAYRALPSFRGDSAFYTWLYRIGINTAKNFLVSQGRKVPTIEGNFD
                     NEDAENFEEAAKYKEVSTPESELMSKQVAQTVNKTLDSLPEELRTAIVLREIEGLSYE
                     DIASMMGCPIGTVRSRIFRAREAIAEKLRPLLGTSKDKRW"
     gene            1307..1858
                     /locus_tag="PKZ49_02155"
     CDS             1307..1858
                     /locus_tag="PKZ49_02155"
                     /inference="COORDINATES: protein motif:HMM:NF015810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HND35393.1"
                     /translation="MMKEKMSELMDGELDHRNIDSVFTEFKRNDELIKDWETYHMISD
                     AIKRPASFQSFDIADKVRKQLNHEPIIFVPNASKVAKHKLYTLSAAASVAVLVGSWLL
                     LQIENIQPVTTVAEKTKERILVSQSPASQPTPTFTYTLPSLEYHYFHHQPQPLARKVF
                     LPNGAMYGPLTSEYQVPEAGNSR"
     gene            2018..3412
                     /locus_tag="PKZ49_02160"
     CDS             2018..3412
                     /locus_tag="PKZ49_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HND35394.1"
                     /translation="MQKFLLSLLFVTSIGIVNAKELPDFTGLVEQYGAAVVNISTIQT
                     QQISGGQIFPDIPNLPEDSPFYDFFRRHMQPFSAPRKFESRSLGSGFVISKDGYILTN
                     AHVVESANEITVKLNDKREFNAQVIGTDRKTDIALIKIDAKDLPVVTQGDPENLKVGE
                     WVIAIGSPFGFENSVTAGIVSAKGRSLAQENYVPFIQTDVAINPGNSGGPLFNMKGEV
                     VGINSQIYSRTGGFMGLSFAIPINVATEIADQLKEHGKVSRGKIGVMIQEVTEELAES
                     FGLSKATGALVVSIEKGGPADKAGIEVRDIILEFNGKPIANSSDLPRIVGNTKPKSEV
                     TTQVWRKGSTQSLTVLVGEMPIEDKSERIQKQGKVPDKANRLGLALSELTDKLKKQLG
                     VEGGLLVEEVQPGTASRVGIRAGDIILGLNSQDIKTVKQFNQMLDKISKGKNIALLIK
                     RGDMTTFITMKLDN"
     gene            3442..3699
                     /locus_tag="PKZ49_02165"
     CDS             3442..3699
                     /locus_tag="PKZ49_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HND35395.1"
                     /translation="MDQPNLIVYVREACHLCQEMVALLHQYQQKTAFKLEIIDIDDDP
                     RLVQQYNELIPVLISADNQQSEICHHHLDITALDAYFNKIR"
     gene            3813..5603
                     /gene="lepA"
                     /locus_tag="PKZ49_02170"
     CDS             3813..5603
                     /gene="lepA"
                     /locus_tag="PKZ49_02170"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HND35396.1"
                     /translation="MRNIRNFSIIAHIDHGKSTLADRIIQYCGGLSEREMEDQVLDSM
                     DLERERGITIKAQTVALKYVGRDGQAYLLNLIDTPGHVDFSYEVSRSLAACEGALLVV
                     DASQGVEAQTVANCYTAIEQGVEVLPVLNKIDLPAADPDRVISEIEDIIGIDAKHALR
                     VSAKTGEGIQDMLEAIIDRIPEPQGDSSAPLKALIIDSWFDNYVGVVVLVRVVDGVLK
                     PGDKIMLMATRATQLCEQVGVFLPKAEHRDALSAGEVGYVISGIKELKSAKVGDTITL
                     VDSPANEPLTGFKEIKPQVFAGLYPVESNQYEALRAALEKLQLNDSSLQYEPETSQAL
                     GFGFRCGFLGLLHLDIVQERLEREYDMDLITTAPTVIYQVLMRDGSEIEIENPSKMPD
                     LSKIAEIREPIITTTILVPEEYVGTVMLLCNGKRGVQRNMQYMGRQVMLVYEMPLNEV
                     VMDFFDKLKSVSRGYASLDYEFKEFRASDLVKLDILINAEKVDALSLIVHRSSSQYRG
                     RELAQKMRELIPRQMFDIAVQAAIGAHVIARENIKALRKNVLAKCYGGDITRKRKLLE
                     KQKAGKKRMKRVGNVEIPQSAFLAILQVDK"
     gene            5711..6514
                     /gene="lepB"
                     /locus_tag="PKZ49_02175"
     CDS             5711..6514
                     /gene="lepB"
                     /locus_tag="PKZ49_02175"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HND35397.1"
                     /translation="MNFPLVLLILLVITGTIWLIDFFILKHKRTVDSAEPWWVEYPKS
                     FFPIILIVFCLRSFLVEPFKIPSGSMIPTLLVGDFILVNKYIYGIRLPVINQKIIDID
                     LPKRGEVLVFRFPEDTSIDYIKRVIGVPGDTVTYRNKQLSINGTPLKLEQDGDYKYIE
                     SGLAFIYAQRFKEINGTGSYSIIINHDIPPLHLSAVHSFPYRDNCVFDRTGFTCNVPE
                     GNYFTLGDNRDGSSDSRYWGFVPEKNIVGKAFLVWWNFSDLSRIGLSIE"
     gene            6548..6916
                     /locus_tag="PKZ49_02180"
     CDS             6548..6916
                     /locus_tag="PKZ49_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HND35398.1"
                     /translation="MYPSTSRKQQGISLSGLMIWSFVLILVSIVGMKLIPAYIENATI
                     KRTLLAVATESNLRTNNEEIRQSFNRRASIDNIKAINGQDIIIDKKSDKVVLSTSYSV
                     KIPLFANVSLYIDFTAASNQ"
     gene            6913..7635
                     /gene="rnc"
                     /locus_tag="PKZ49_02185"
     CDS             6913..7635
                     /gene="rnc"
                     /locus_tag="PKZ49_02185"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HND35399.1"
                     /translation="MILTSSPNTSPKLSEKTRFNLFFETIGYSFKELTLLREALTHRS
                     FGLPNNERLEFLGDSVLNCAISGLLFKKFPTITEGDLSRIRSNLVNQSTLFNLALSIG
                     IADLIRLGEGEKKSGGNNRPSILANALEAILGAVFLESGFKKTETCIKKIYAPLLQTL
                     EFDAIDKDAKTTLQEHMQSLKLDLPEYIVLAVSGLAHRQKFMVECVIAKLNIRVVGEG
                     TSRRRAEQDAARQAYALATQSG"
     gene            7713..8609
                     /gene="era"
                     /locus_tag="PKZ49_02190"
     CDS             7713..8609
                     /gene="era"
                     /locus_tag="PKZ49_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HND35400.1"
                     /translation="MNNSPQFRTGYVAIIGKPNVGKSTLLNFLIQQKISITSKKVQTT
                     RHRIKGILTDQQSQFIFVDTPGYQVRYSNKLNTIMNKIVIQSINEVDVVLFVIEAINF
                     DSNDELILRQLPANKPVILVINKIDQLSDKSKLLPFLAQISTKYPFTEMVPISAIKKM
                     QLTQLLGAVRAYLPENPPLFGEDEICDRNERFLAAEMIREKLFRLMGDEIPYSASVSI
                     DRFEQLTHIRKIYACILVDKTSQKIMIIGQGGEKLKQIASLARKDMEKLFGSKVYLEV
                     WVKVKAGWANDAKTLSGLGYEG"
     gene            8643..9368
                     /gene="pdxJ"
                     /locus_tag="PKZ49_02195"
     CDS             8643..9368
                     /gene="pdxJ"
                     /locus_tag="PKZ49_02195"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041353082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HND35401.1"
                     /translation="MVTLGVNIDHVATLRQARKTIYPSPVMAALIAESAGADAITLHL
                     REDRRHIQEYDVETLRQQLKIRMNLECAITEEMVTFACRIKPHDVCLVPERREELTTE
                     GGLDVVRYFKQVKTVCSRLADAGIFVSLFIDAELDQIETAVKAGAPAIEIHTGPYADA
                     KDSDDQQSELEKICAAVAYGNRLKLKVNAGHGLHYENTRPIAAIEGISELNIGHAIVS
                     HALFVGFEQAVKQMKTLIQEASQ"
     gene            9365..9742
                     /gene="acpS"
                     /locus_tag="PKZ49_02200"
     CDS             9365..9742
                     /gene="acpS"
                     /locus_tag="PKZ49_02200"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HND35402.1"
                     /translation="MIYGIGTDLVKPSRITRSLEKYGERFARRLLSEWEWQDYVNNNQ
                     PALFLAKRFAAKEALSKAMGTGLRPPVTLTNIAIAHDSLGKPYFEFHPELATLIKNEG
                     ITHHHLSISDELNLATAFVILER"
     gene            9745..10794
                     /gene="nagZ"
                     /locus_tag="PKZ49_02205"
     CDS             9745..10794
                     /gene="nagZ"
                     /locus_tag="PKZ49_02205"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HND35403.1"
                     /translation="MSLGPLMLDIGSTQLTQAEITRLKHPLTGGVILFARNFESSKQL
                     IELTAEIRSIRNPQLLIAVDHEGGRVQRFQKEFTRLPAMHELGKIWNHRPQQAKHLAK
                     QIGFVMSAELSACGIDFSFTPVLDVDHGQSSVIGDRAFHRSSSAIAELAHSLMIGLKL
                     NGMSAVGKHFPGHGFIKADTHHELAIDPRIYQDIEMEDLIPFKQLIDFGITGMMAAHV
                     IYPNVDKHPAGFSSYWLKTILRNELGFEGCIFSDDLSMKGTEGYGNIVDRTRISLEAG
                     CDMVLVCNDSLSCDLLLDQLKWETSAISLARLARMRGRSHASSLIKLHEEASFVQAVN
                     EIDQIGKDNLELSFT"
     gene            10848..12314
                     /gene="lpdA"
                     /locus_tag="PKZ49_02210"
     CDS             10848..12314
                     /gene="lpdA"
                     /locus_tag="PKZ49_02210"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HND35404.1"
                     /translation="MAKLFDVAVIGAGPGGYVAAIRCAQLGLNTVCIDEWKNSEGTPS
                     LGGTCLNVGCIPSKALLESSENFERAAKKFGEHGIKLENLSIDVPAMIARKNKIVKAF
                     TSGIAMLFKKNKITSMHGRGMLLDRQNENQLWNIQVSNGDQSEVVQAEFVILATGSTP
                     RPFPLVEIDNDKVLDNVGALALSEVPKRLGVIGAGVIGLEMGSVWRRLGAEVTILEAQ
                     PGFLLSADESVAKEAQKVFTRELGLVIQTGVDINSIKQNKQQISIAYTDSNKESQKLE
                     VDKLVVAIGRVPNSKGLASDGNSLSVNDQGFIEIDVHCRTNLPNIFAVGDVVRGPMLA
                     HKASEEGVAVAELIATVKKQAANNESLPKVDLTVVPWVIYTSPEIAWVGKTEQMLKAE
                     NIPYKVGQFPFMANGRARALGETHGFVKILAHAETDRILGVHMIGPFVSELIAEAVVA
                     MEFKASSEDLARIIHAHPSLSEVLHEAALGVDKRALHI"
     gene            12401..13468
                     /locus_tag="PKZ49_02215"
     CDS             12401..13468
                     /locus_tag="PKZ49_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35405.1"
                     /translation="MGIEQLNNLIGKWVSGFSINVITDRTWVELASILAPSALLILIL
                     FGIAVSLENKTWRSFNLWICLLVTIFYLPYELYRQSSNLVKAQTNIGEVQTNLKLLLD
                     SAHLNHVGNLIDEEVSSHVLGEMIHSGEKKDLLYISWLLSENEKKLLAEQENKQKMIV
                     NELNSALSNLKQEIVETQEPVEKIANDILSRIDGTISQLIESKIQHFNQALNQSLENF
                     QSEINTFVQNELNTYQAKLTEITQQNTDELQSYTRSAKQTFADQINQTNKESLQKLDE
                     TTKSIDQMKAALGGINFEQVVGQVQQLSNSIKSAQERNEALFEYNECIRTAGIIDLIG
                     KEEDCKKKLNNRLNELTHSNK"
     gene            13599..13964
                     /locus_tag="PKZ49_02220"
     CDS             13599..13964
                     /locus_tag="PKZ49_02220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HND35406.1"
                     /translation="MNKRFLSQLIISLGICSLLLIPMTVFAEEGTAVHYSNKFQGRKT
                     ASGEVFDQKKLTAAHKQYPFGTKVRVTNLENNRSVIVKINDRMATKNSNIIDVTSRAA
                     NELGFRKKGKAQVSLEAVD"
     gene            complement(14129..15667)
                     /locus_tag="PKZ49_02225"
     CDS             complement(14129..15667)
                     /locus_tag="PKZ49_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HND35407.1"
                     /translation="MNNPTPVFSTSQIREAERIAINQLNTPSLMEQAGLAAATLAKEA
                     MLTSTMQRVLIVAGPGNNGGDALVVARHLKNWGYPVTVIFTGNESKLSVEAKQAFEKW
                     IAVEGTIVQCIPTNEQWDIIIDGLFGIGLTSTRPLCDDYQALIHSINQMKLPVLSLDV
                     PSGLDADTGNIAGAAIKATITATFIGLKPGLLTHDGCNYSGKIVLCNLGLNSTILVTP
                     HSWLLNFSFIKSCLPTPRLINSHKGTYGSVGVIGGTAGMVGAAILAGTAALKLGAGRV
                     YIGLIAKEALSVDVQQPELMLHSIDHLFELNQINSLIVGPGLGTELAACIYLEKALQL
                     SIPIILDADALNLIARYPELQELVCSRQLATILTPHVAEAARLLQVDTATVQANRINA
                     TENLVKKFNCAVVLKGAGSICGFPNGYIYFNTTGNPGLSSAGTGDILSGMIAALIAQN
                     LKPENALLLAVYLHGAAADALLKTLHGPIGMTATEIIDSARCLINQWVYGTDVSSRYL
                     RLFL"
     gene            15727..19725
                     /gene="purL"
                     /locus_tag="PKZ49_02230"
                     /gene_synonym="purI"
     CDS             15727..19725
                     /gene="purL"
                     /locus_tag="PKZ49_02230"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HND35408.1"
                     /translation="MLQFRGRQALSSFRLGKLSHQIRSIVSEFIKIESEYWYFCSLQQ
                     NLTQNELIRLQQLLDGHLIETLPQVPHGFFLVLPRLGTVSPWSSKATDIAHHCQLQSI
                     GRIERGVAFNIEFQSHITDESRKRVEALLHDRMTESIFSSLQEASKLFRHIDPGSLQT
                     IDIQTAGSMALQHANQAMGLALSPDEIDYLADYFLKQSKRNPTDVELMMFAQANSEHC
                     RHKIFNADWAIDGVPQTQSLFSMIRHTHQQTPQGTLIAYSDNAAVIEGDIIDRFYPGK
                     SNVYGYEKELTHLLMKVETHNHPTAISPFPGAATGVGGEIRDEGATGRGAKPKAGLTG
                     FSVSNLRIPDFVQAWEVESEWGEAGYGKPDRIASALDIMLAGPIGGAAFNNEFGRPNL
                     AGYFRTFELSMNGVMRGYHKPIMLAGGLGQIADKHTTKQSFSSDVLLIQLGGPGLLIG
                     LGGGAASSMDTGTNAEDLDFDSVQRGNPEMQRRAQEVIDRCWQLEKIGQANPILSIHD
                     VGAGGLSNALPELVYGAGCGGKFDLCAIPSEESGMSPMQIWCNEAQERYVLAIKRESL
                     SLFHAICERERCPYAVVGESKQVDQLLLTDSRSTIPPIDMPLSVLLGKPPKMQRTVVH
                     VDNPLKPIDFSHIQLKDAIYRILRLPTVADKSFLITIGDRSIGGMTARDQMVGPWQIP
                     VADVAVTAMGYQTHLGEAFAIGERTPLALINAAASARMAIGEALTNIAAASIERLDYI
                     KLSANWMAAAGHANEDAALFDAVHAAAIALCPQLGISIPVGKDSMSMKTTWWQENNVG
                     ENQKKEVIAPLSLIISAFARVKDVRQTLTPQLRNDLGETALILIDLGFGKNRMGGSAL
                     VQVYNQLGDQAPDLETNPEIEKFKSFLGVIQQLKESNQIVAYHDRSDGGLFVTLCEMV
                     FAGHIGVTINLDQLCFDPSASDLDGAEIKPEALQGRFFERLLSVLFNEELGAVIQIQD
                     VHHQAVLQAFAEVGLANHCFVIGKLNQSDEIQILRNNIAVIREKRVDLHRAWSETSYH
                     MQRIRDNPACAQQEYDRILDTADPGLHSKLTFNVHDDIAFPSIQMRSRPKIAILREQG
                     VNGQVEMAAAFDQASFSAVDVHMSDILAGRDLLTDYKGLVACGGFSYGDVLGAGKGWA
                     KSILFNPVARDAFQTFFNRQDTFALGVCNGCQMMSNLEEIMPGTAHWPRFERNQSEQF
                     EARFILVEIPESPSILFKGMEGSRLPITVAHGEGFASFQTSQQLATASHYIALRYIDH
                     YGNPTERYPFNPNGSALGITGLTSVDGRFTILMPHPERVFRTIQHSWHAEDWPTYSPW
                     MKMFRNARQWVG"
     gene            19800..21068
                     /locus_tag="PKZ49_02235"
     CDS             19800..21068
                     /locus_tag="PKZ49_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HND35409.1"
                     /translation="MTNNKNKHKLPLNLSMRVWFALIVILSSNASAEIIPLVKPIQTS
                     RAASSTKQCVPVASWVIPGKNVKTSFTEIASHANQHSVILLGETHVNREHHRWQLQML
                     AALHTIRPDMIIGFEMFPRSAQKALDQWIAGELSESDFLQKTNWNSVWGTDASLYLPL
                     FHFARMNRIPMRALNIDSELRRRVSEKGFDSVPENEREGVTRPAPPSAAYLDFLLPIY
                     ELHDRKHKQQAKSNKYDPDFLRFVVGQQLWDRAMAQEIKAALDLLEKRKNSLVVGIMG
                     TGHILHGFGVPHQLKDLGVNGITTLIPWDTNKSCKQLTDGYADAVFGLTPQNIEVPPS
                     FQRLGIRYEMMVEQGGARVMEVEKASIAESAGLLTGDIIREIAGVAVKDTNDIVTVVK
                     RQAPGTWLPISIERGKELKQIIAKFPALAK"
     gene            complement(21292..>21541)
                     /locus_tag="PKZ49_02240"
     CDS             complement(21292..>21541)
                     /locus_tag="PKZ49_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HND35410.1"
                     /translation="STIIPLNILMNTEMEKADLEAFLRSLTDERVRYRKAPFDHPQLF
                     IPVGYKPDSNSAKNIKEIFLEIEAVGREGGKPLKPFLR"
BASE COUNT         6588 a         3914 c         4736 g         6303 t
ORIGIN      
        1 tccgatctcc taaccacatc gcagcaacta tcacggcaca tcatttgtta tttaatcgta
       61 atgcaatttt taccagtggc attcggccac attactactg tttaccgatt ttaaagcgtg
      121 agaaacatcg gcagtcactt gtcgcagcag cgattagtgg ttccccaaaa tttttcttag
      181 gtaccgatag tgcaccgcat tcaatcgcaa ataaagaaag taattgtggc tgtgcaggaa
      241 tctattctgc acatgcggga atcgaatttt atgttgaagc gtttgatcgg gctggtgccc
      301 tagacaaact tgaagctttt gcgagttttc atggtgcaga tttttatcag ctaccgagaa
      361 acgaacatca aataacgttg attaagcaag attggataat acctgcgcaa attcgttttg
      421 gtgatgaaca attgattccc ctaatggcgg gtgaaaagct ttcctggaaa ctgagtaatc
      481 attttttagc atggtaggcc acgtaaatca taataagcac ctaatttatc agattattta
      541 gcattttctg cggtgaacta attcctaata cagattgtct tttagttgat aggtcgtaca
      601 cgatcgtttc ctgaaaaaag gtatactttc aaattctgtt tgaaaacgag tttagaaaag
      661 gatcaatatc ttaatgttta ggaaacaaga agtatgagtg atcgcgaaat cgatcaacag
      721 ctcgtggagc gtgttcagca aggtgataaa agggcgtttg atttattagt cgtcaagtat
      781 cagcgcaaat tggctcgact attgtcacag ctcattcgcg atcctgtaga agtcgaggat
      841 attgcccaag agacttttat taaagcgtat cgtgcactgc cttcatttcg aggtgatagc
      901 gctttttata cttggctata ccgaattgga attaatacag caaaaaattt tcttgtgtca
      961 caaggtagaa aagtaccgac gattgaaggt aattttgata atgaggatgc tgaaaatttt
     1021 gaggaagcgg ctaaatataa agaagttagt acacccgaaa gtgaactaat gagtaaacaa
     1081 gttgcccaaa cggttaataa aacgctcgat tccctacccg aagagctaag gactgcgatt
     1141 gtactacggg aaattgaagg gttaagctat gaagatattg catcaatgat gggatgtccg
     1201 atcggaacag tgcgttcacg tatcttccgc gcacgtgaag caattgccga gaaattacgt
     1261 cccttactag ggacgagtaa agataaaaga tggtgatgga gagaatatga tgaaagaaaa
     1321 aatgtctgag ctgatggatg gtgaactaga tcatcgcaat atagattcgg tgtttacaga
     1381 atttaagaga aatgatgaac taatcaagga ttgggaaaca taccatatga ttagtgatgc
     1441 gattaaacga cctgcttcgt ttcaatcatt tgatattgct gataaagtta gaaaacaact
     1501 caatcatgag cctattattt ttgtgccgaa tgcatcaaaa gttgcgaagc ataaactcta
     1561 taccctttcg gcagctgcct cggtagcagt tttagtaggt agctggttgc tgctacagat
     1621 agaaaatatt caacccgtta caaccgttgc tgaaaaaacc aaagaaagaa ttctagtttc
     1681 tcaatctcct gcatctcagc caaccccaac atttacttac acacttccat ctttggaata
     1741 ccattatttt catcatcagc ctcaaccatt agctcgaaaa gtttttttac ctaatggcgc
     1801 gatgtatggt cctttgacaa gtgaatatca agtcccagaa gctggaaact caagatagta
     1861 aggatctctg agtgaacggc attaaaatat caatgccgtt actttttctt taagatagaa
     1921 tctaagtaga tagatttcta gtcattttat ttatatttta cagcactact cttaaactaa
     1981 ttagtaactt taacttgatt caaaatagca gagatttatg caaaaatttt tactgagttt
     2041 attgtttgtt acttcaattg gaattgtaaa tgcaaaagaa ttgcctgatt tcacagggct
     2101 cgttgagcaa tatggagcgg cggttgttaa tattagtaca atccaaacgc agcagatatc
     2161 tgggggtcag attttccctg atattcctaa tctgcctgaa gactcccctt tttatgattt
     2221 ttttcgcagg catatgcaac cgttttctgc gcctagaaaa tttgaatcgc gctccttggg
     2281 ttcagggttt gttatcagta aggatggtta tatcttaacg aatgcacatg tcgttgaatc
     2341 agcaaatgag attacagtaa aacttaatga taagcgtgaa tttaacgcac aagtaattgg
     2401 tactgatcgt aaaacggata ttgcattgat taaaatcgat gcaaaagatt taccggttgt
     2461 gacgcaaggg gatcctgaaa atctaaaagt aggggaatgg gttattgcga tcggttctcc
     2521 gtttggattc gaaaatagtg taacagcggg tatcgttagt gcaaagggga gatcgctcgc
     2581 gcaagaaaat tatgtacctt ttattcaaac cgatgttgca attaatccgg gtaattcagg
     2641 tggtcccctt tttaatatga aaggggaagt cgttggtatc aactcacaga tctatagtcg
     2701 tacgggtggt tttatgggat tgtcttttgc tattccaatc aatgtagcga ccgaaattgc
     2761 tgatcagtta aaagaacatg gaaaagtatc acgtgggaaa attggcgtta tgattcagga
     2821 agttactgag gagttagctg aatcatttgg tttatcgaaa gcgaccggcg cgttggttgt
     2881 atcgattgaa aaaggagggc cagcggataa agcaggaata gaagttcggg acattattct
     2941 cgaatttaat gggaaaccaa ttgcaaattc tagtgatctt cctcgtatcg ttggtaatac
     3001 aaagccaaaa tcagaagtga caacgcaagt ttggcgtaaa ggttcaactc agtcgcttac
     3061 ggttcttgtc ggtgaaatgc ctattgaaga taagagtgaa cgcattcaaa aacaaggtaa
     3121 agtacctgat aaagccaatc gacttggcct tgctttaagc gaattaacag ataagctaaa
     3181 aaaacaactg ggcgtggagg gtggactact tgttgaagaa gttcaaccgg gaacggctag
     3241 tagggttggt atacgcgctg gagatattat tctcggattg aatagtcaag acattaaaac
     3301 agttaagcaa tttaaccaaa tgctagataa aattagcaag gggaaaaata ttgcgttatt
     3361 gattaaacgc ggtgatatga ccacatttat taccatgaag cttgataatt aatcttaatt
     3421 tcaaatttaa agaaaacatc tgtggatcaa cctaatttaa tcgtttatgt aagagaagca
     3481 tgccatttat gtcaggaaat ggttgcgcta ttgcaccaat atcagcagaa gactgcattt
     3541 aaattggaaa tcatcgatat cgatgatgat cctcggttgg tccagcaata caatgaactt
     3601 attccagtac taatcagtgc agataatcaa caaagcgaga tttgtcacca tcaccttgat
     3661 attactgcat tagatgctta tttcaataaa attaggtaga atccacttcg ctgattaatt
     3721 tcaacgagat tgtgtaagta agcataaaca tggtaaagct tacttacacg catcaacaac
     3781 aactcaacta aaataagctt ttttcttccc ggatgcgaaa cattcgtaat ttttccatta
     3841 ttgcgcatat tgatcatggt aaatccacat tagcggaccg aatcattcaa tattgtggtg
     3901 gtctttcgga acgtgaaatg gaagatcaag ttctcgattc gatggatctt gagcgggagc
     3961 gtggcattac cattaaagcg caaacagtag cgctaaaata tgtgggtcgt gatggtcagg
     4021 cttatttatt gaatttaatt gatacaccag ggcatgtcga tttttcttat gaagtatccc
     4081 gatcattggc agcgtgtgaa ggggccttgc tcgttgtaga tgcttcacag ggtgttgaag
     4141 ctcaaacagt agcgaattgc tacacggcaa tcgaacaagg tgttgaggtc ttgcctgttt
     4201 taaacaaaat cgatctacct gcagcagatc ctgatcgagt catatctgag attgaagata
     4261 ttatcggtat cgatgctaag catgctctaa gggtgagtgc taaaacgggt gaaggcattc
     4321 aagatatgct tgaagcgatc attgatcgaa ttcctgaacc gcagggagat agcagtgctc
     4381 ccctcaaagc gctcattatt gactcttggt ttgataatta tgtaggcgtt gttgttctcg
     4441 ttcgcgtagt ggatggtgtt cttaaacctg gggacaaaat tatgctaatg gcgactcgag
     4501 ctacacaatt atgtgagcaa gtgggtgtat tcctacccaa agcagagcat cgtgatgcgc
     4561 tgagtgcagg agaagttggt tacgttattt ctggaatcaa agaattaaaa tcagcaaaag
     4621 taggggatac tataacgcta gtcgattctc cggcgaatga acctttaact ggatttaaag
     4681 aaatcaagcc ccaggttttt gcaggtcttt atcctgtcga atccaatcaa tatgaagcat
     4741 tgcgggctgc gctagagaaa ttgcaattga atgactcttc attgcagtat gaacctgaaa
     4801 catcccaagc attaggcttt ggatttcgtt gtggttttct aggcttactg cacttagaca
     4861 ttgtgcaaga gcggttagag cgtgaatatg atatggacct cattacaacg gctccgactg
     4921 ttatttatca ggtattgatg cgcgatgggt cagagatcga gatcgaaaat ccttccaaaa
     4981 tgccggatct ttcaaaaatt gcggaaatta gagagcctat aattacaacg actattcttg
     5041 tacctgaaga atatgttggc actgtaatgt tactatgcaa tggtaagcga ggcgttcagc
     5101 ggaatatgca gtacatgggt aggcaagtaa tgctggttta tgaaatgcct ttaaatgaag
     5161 tagttatgga tttttttgac aaattgaaat ccgttagccg aggttacgca tctcttgatt
     5221 acgaatttaa ggaatttcgt gcatccgatt tagtaaagct tgatatattg attaatgctg
     5281 aaaaagttga tgcattatca cttatagtcc atcgctcaag tagtcagtat cggggccgcg
     5341 agttagcaca aaaaatgcgt gaactcatac cgcgtcaaat gtttgatatt gctgtacaag
     5401 cagcaattgg cgcacatgtc attgctagag aaaatatcaa agcgttacgc aagaatgtat
     5461 tagcaaaatg ttacggtggg gatattacac ggaaacgcaa gctacttgaa aaacaaaaag
     5521 cgggtaagaa acgcatgaaa cgtgtaggta acgtagaaat ccctcaatcg gcctttttag
     5581 caattttaca ggttgataaa tagctgattg ccacaaaata tgcgatttac aagttaaaat
     5641 atgccaatat tgaataacgt gattatcttg taatgcagtc cgaatcatca ttttttgatt
     5701 ttaacgataa atgaactttc ccttagtgct tcttatcctc ttggtcataa cgggtacgat
     5761 atggctgatt gattttttta tacttaaaca taaacgtacg gttgattcgg ctgaaccttg
     5821 gtgggtcgaa tatcctaaaa gttttttccc aattattttg attgtatttt gtcttcgctc
     5881 atttttagtc gagccattta aaatcccatc aggttctatg attccgacgt tgttggtggg
     5941 cgattttatt ttagtaaaca aatatattta tggtattcgc ttaccggtaa ttaatcagaa
     6001 gataattgat atcgatttgc cgaagcgcgg tgaagtattg gtgtttaggt ttccagagga
     6061 cacttcgata gattatatta agcgtgtaat cggtgtgcct ggggatactg ttacgtatcg
     6121 aaataagcaa ttgagtatca atggtactcc cctaaaatta gaacaagatg gggattataa
     6181 gtatattgaa tcaggacttg cgtttatcta tgcacagcga tttaaagaaa tcaatggaac
     6241 aggtagttac agtatcatta tcaatcatga tattcctccc ttacatttat cagccgttca
     6301 ttcttttcct tatcgagata attgcgtatt tgatcgcact ggttttactt gcaatgtacc
     6361 tgaaggaaat tattttacgt tgggtgataa tcgggatggc agtagtgata gtcgatattg
     6421 gggattcgta ccggagaaaa atatcgttgg gaaagcattt ctagtttggt ggaattttag
     6481 tgatctcagt agaattgggc tttcgattga ataataaagt taattattaa ttgaaatgag
     6541 gagttatatg tatccttcaa catcgagaaa gcagcaagga attagcttat cgggattaat
     6601 gatttggtca tttgtattga ttttagtatc tattgtcggg atgaaattaa tccctgcgta
     6661 tatcgaaaat gcaacgatta aacgtacctt acttgcagtc gctacggagt ctaatttaag
     6721 aacgaataat gaggaaattc gtcaatcttt taatagaaga gcgagtatcg ataatattaa
     6781 agcgattaac ggacaagata ttattatcga taagaagagt gataaagttg tactgagcac
     6841 gagctattct gtgaaaattc cattgttcgc caatgtttcg ttatacattg attttactgc
     6901 tgcaagtaat caatgatttt aacgtcttct cctaacacgt ctcctaaatt atctgaaaaa
     6961 acaagattca atcttttttt tgaaaccatt ggttactcat tcaaagagtt aaccttactg
     7021 cgggaagctc tgacacatcg aagttttgga ttgcccaata atgaacggtt ggagtttcta
     7081 ggggatagcg tattgaattg cgctatatcg ggattacttt ttaagaaatt tcctacgatc
     7141 actgagggag atttatcgag aatccggtca aatcttgtaa atcagagtac acttttcaat
     7201 ctagcattat ccattggaat cgctgatttg atccgcttag gagaggggga aaagaaaagc
     7261 ggtggtaata atcgaccttc tattttagca aatgcgctcg aggccatcct tggtgctgtt
     7321 tttcttgaaa gcggcttcaa aaaaactgaa acatgtatta aaaagatata cgcgccttta
     7381 ttacaaacac tagaattcga tgcaattgat aaagatgcta aaacaacgtt acaagaacat
     7441 atgcaaagtt taaaacttga tttaccggaa tatatcgtgc ttgcggtaag tggtttggcg
     7501 catcgccaga aatttatggt tgaatgtgtg attgccaagc tcaatataag agttgtgggt
     7561 gaaggaacca gtcgtcgcag agctgagcag gatgcggcaa ggcaagctta cgcgctagca
     7621 acacaatcgg gttgattgac agcgactaat ctgatttagt ctagcaaaac cttctaagaa
     7681 aacgatatat aaacagccta tatacccaat taatgaacaa ctcacctcaa tttaggactg
     7741 gttatgttgc gattattggt aaaccgaatg taggtaagtc cacattactt aattttttga
     7801 tccaacaaaa aattagtatc acttctaaga aagttcaaac gacgcgccat cgcatcaaag
     7861 ggattctaac ggatcagcaa tcacaattta tttttgtaga tactccaggt tatcaggttc
     7921 gctattcgaa taaattaaat actataatga acaaaatagt gattcagagc atcaatgagg
     7981 tcgatgttgt gttgttcgtc attgaagcga tcaattttga tagtaatgat gagttgatat
     8041 tgcgtcagtt acctgccaat aaacctgtta tattggtaat taataaaatc gatcaattga
     8101 gcgataaatc aaaacttcta ccttttcttg ctcaaatttc aacaaaatat ccatttaccg
     8161 aaatggtgcc gatcagtgcg atcaaaaaaa tgcaattgac tcagctgctc ggagcagtta
     8221 gagcctatct tcctgaaaat cctcccttat ttggtgaaga tgaaatatgc gatcgtaacg
     8281 aacgttttct agctgctgaa atgataagag aaaagttgtt tcgattaatg ggagatgaaa
     8341 ttccatattc agcgagtgtt tcgatcgatc gattcgagca gctaacccat attcgaaaaa
     8401 tttatgcttg tattttggtt gataaaacca gtcaaaaaat aatgattatt gggcaaggtg
     8461 gcgaaaaatt gaagcagatt gcatcactag ctcgaaaaga tatggaaaaa ttatttggaa
     8521 gcaaagttta tttagaagtt tgggtgaaag tgaaagcagg ttgggccaat gatgcaaaaa
     8581 cgttaagtgg actcgggtat gaaggctaat ttagctattt taataatagg actaaatctt
     8641 aagtggttac actaggtgtt aatatcgatc atgtcgcaac tttgcggcag gcaaggaaaa
     8701 caatttatcc aagcccagtc atggctgcgt tgatagctga atcagcgggt gctgatgcca
     8761 ttactctcca tttaagggaa gatcgacgtc atatccaaga atatgatgtt gaaacattac
     8821 ggcagcaact taaaattcgt atgaatttag agtgcgcgat cactgaagag atggttactt
     8881 ttgcatgtcg aattaagccc catgatgtat gtttagtgcc tgaacgacgt gaagaattga
     8941 caacggaagg aggattagat gttgttcggt attttaagca agtcaaaaca gtttgtagcc
     9001 gattagcgga tgctggaatc tttgtttcat tgtttattga tgccgaattg gatcaaattg
     9061 aaacagcagt taaagcaggc gcacccgcaa ttgaaattca tacgggaccc tatgccgatg
     9121 ctaaagactc tgacgatcag caaagcgaat tagagaaaat ctgtgcagcg gtagcctatg
     9181 gcaatcgtct caagctgaag gtaaatgccg ggcatggttt acattatgaa aatacccgtc
     9241 ctatcgcagc catcgaaggt atttcagaat tgaatattgg ccatgcgatt gtatcgcacg
     9301 ccttatttgt aggctttgaa caagctgtga agcaaatgaa aacattaatt caggaagcaa
     9361 gccaatgatc tatggcatag gcacagatct tgtaaaacct tctcgtataa cacgatctct
     9421 cgagaaatat ggagaacgtt ttgctagacg cttactatct gaatgggagt ggcaggatta
     9481 cgtcaataat aatcagcccg ccctttttct agctaaacgc tttgctgcga aagaggcgct
     9541 gtcaaaagcg atggggacgg gtctgcgccc cccagttacg cttacgaata tagcaattgc
     9601 ccatgattcg ttaggtaaac cctattttga atttcaccca gagttggcta cgttaattaa
     9661 aaatgaggga atcacgcatc atcatttatc gattagcgat gagttgaatc tagcaaccgc
     9721 ttttgtgata ctggaaagat aaatatgtca cttggtcctc tgatgcttga cattggaagc
     9781 acgcaattga cccaagcaga gattacgaga ttaaagcacc ctttaactgg tggagttatt
     9841 ctttttgcgc gtaattttga atcgagtaaa caattaatag aactgactgc agagattcgt
     9901 tcaataagaa atccacagct attgattgcg gttgatcatg aagggggcag agtacagcga
     9961 ttccagaaag agtttacgcg attaccggcc atgcatgagc ttggaaaaat atggaatcat
    10021 cgtccacaac aagcgaagca cttagcaaaa cagattgggt ttgtgatgtc tgctgaattg
    10081 tccgcctgtg gtattgattt tagctttaca ccggtactcg atgtagatca tggacagagt
    10141 agtgttatcg gtgatcgtgc gtttcatcgg tcatcttctg ctatcgctga gttagcacat
    10201 agtttgatga tcggtttaaa attgaatggt atgtctgctg ttggaaaaca ttttccaggt
    10261 catggattta ttaaagcaga tacgcaccat gaactcgcga ttgatcctcg gatctaccag
    10321 gatattgaga tggaagacct catccccttt aaacaactca ttgattttgg aatcacggga
    10381 atgatggcag cacacgtcat ttatcctaac gttgataaac atccagcagg attttccagc
    10441 tattggttaa aaactatttt acgcaatgaa ttgggatttg aagggtgtat atttagcgat
    10501 gatttgagta tgaaaggtac ggaaggctat ggcaatatcg ttgatcgtac tcgaatatcc
    10561 ctagaagcgg gttgtgatat ggtattggta tgtaatgatt ctttatcctg cgatttgctg
    10621 ctagaccaat taaaatggga aacgtcggca atcagtcttg cccgattagc ccgtatgcgt
    10681 ggccgatctc atgcttcttc actgattaaa ttacatgagg aagccagctt tgttcaagct
    10741 gtaaacgaaa tagaccaaat tggcaaagat aacttagaac taagctttac ttaaaagata
    10801 tttcaaacac tttaaaccta atcgttttta atttataaaa agaaatcatg gctaaattat
    10861 tcgatgtcgc tgttatcggt gcaggaccgg gtgggtacgt tgctgcaatt cgctgtgctc
    10921 aacttggact gaatacagta tgtattgatg aatggaaaaa tagtgaagga acaccgagtt
    10981 tggggggaac ttgccttaat gtgggttgta ttccatccaa agcgttattg gaatcttcgg
    11041 agaattttga acgtgctgct aaaaagtttg gcgagcatgg aatcaaatta gaaaaccttt
    11101 ccatcgatgt accagcaatg attgcccgta agaataaaat tgtaaaagca tttaccagtg
    11161 gaatagcaat gcttttcaaa aaaaataaaa tcacctcgat gcatggccgg ggaatgttac
    11221 ttgatcgcca gaacgagaat caattatgga atatccaagt aagcaatggc gatcaaagtg
    11281 aagtggtcca agctgagttt gttatattag cgaccggatc aaccccccga ccttttccac
    11341 tggtcgagat tgataatgat aaagtgttag acaatgtcgg tgcacttgct ttatctgagg
    11401 tacctaagcg tcttggggtg attggtgccg gtgttatcgg attggaaatg ggaagcgtct
    11461 ggcgtcgttt aggtgcagaa gttacgattc tcgaagcaca acctggtttt ttgttgagtg
    11521 ccgatgagtc agttgctaaa gaagcacaaa aggtcttcac ccgtgaattg ggtttggtga
    11581 ttcagactgg ggttgatatc aactcaatta aacaaaataa acaacaaatt tctatagcct
    11641 atacagattc aaataaagaa tcacagaaac tcgaggttga caaactcgta gtcgcaattg
    11701 gtagagtgcc aaattctaag ggcttagcca gtgatggcaa cagtttatct gtgaatgatc
    11761 agggttttat cgagattgat gtgcattgtc gtacgaattt gcctaatatt tttgcagtag
    11821 gcgatgtagt aagaggaccg atgttagccc ataaagcatc agaagaaggt gttgctgtcg
    11881 ctgaactaat tgctactgtt aaaaaacaag cagctaataa cgaatcttta ccaaaagttg
    11941 atctgaccgt tgttccatgg gttatttata catcgcctga aattgcctgg gtaggtaaaa
    12001 cggagcaaat gctaaaagct gagaatatac cctacaaggt aggtcaattt ccgtttatgg
    12061 cgaatggccg ggcaagagca ctcggtgaaa cacatggttt tgtcaaaatt ttagcgcacg
    12121 ctgaaaccga tcgaatttta ggggtccata tgataggacc ttttgtctca gaattaattg
    12181 cagaggcagt tgtagcaatg gaatttaaag cgagtagtga agatcttgcg cgtattattc
    12241 atgcgcatcc ctcattgtca gaggtgttac atgaagctgc gctaggcgtt gataaacgtg
    12301 ctttacatat ttagggtagg ttaaatattt tcgattggta taaataatca atatcaacaa
    12361 tattagaaaa tagaagagat ttcatagggg agggtagcga atgggaattg aacaattgaa
    12421 taatttgatt ggaaaatggg tgagtggttt ttcaatcaac gtcataaccg ataggacttg
    12481 ggttgagctt gcatccattt tggcgccctc agcattgtta atcttgattt tgtttggtat
    12541 cgcagtttct cttgagaata aaacttggcg atcatttaat ttatggattt gtttattagt
    12601 caccattttt tatctacctt atgagcttta tcgccaatct agcaaccttg ttaaggctca
    12661 aacgaatatt ggtgaagttc aaacaaattt aaaattacta cttgattcgg cccatttgaa
    12721 tcacgtaggc aatctaatcg atgaagaagt gtcttcccat gtgctcgggg aaatgatcca
    12781 ttctggagag aagaaagacc tcctgtatat ttcgtggtta ctgagcgaga atgaaaaaaa
    12841 attgttggca gaacaagaaa ataaacaaaa aatgattgtc aatgaattga attcggcgct
    12901 atctaattta aagcaggaga ttgttgagac ccaggaaccg gtagaaaaaa tagcgaacga
    12961 tattcttagc cgtatcgatg gaacgattag ccagttgata gagagtaaaa ttcaacactt
    13021 taatcaagca ctgaatcagt cattagaaaa tttccaaagt gaaattaata cgtttgtaca
    13081 aaatgagtta aatacttatc aagcaaaatt aactgaaatt acacaacaga atacggatga
    13141 attgcaaagc tatacacgct cagcaaaaca gacttttgca gatcagatta atcaaactaa
    13201 caaagaatct ctacaaaaac ttgatgaaac gactaaaagt attgatcaaa tgaaagctgc
    13261 tctcggtggt atcaactttg aacaggtagt aggccaagta cagcaactat caaattcgat
    13321 aaaatcagcg caagaaagaa atgaagcgtt gttcgaatac aatgaatgca ttcgaactgc
    13381 aggaataatc gatctaatcg ggaaagaaga ggattgtaag aagaaattaa ataatcgtct
    13441 aaacgaattg acgcactcga acaaataagc tgcaaactaa taagcaaccc attttaggga
    13501 tattaacgct tgtcatgaca ggtttattgt tttagtttaa aacagtggtt agaatggctg
    13561 agtaaaataa ataattcaat actagaagga ataataaaat gaataaacgt tttctatcac
    13621 aattaattat tagtcttggc atttgcagtt tgctattgat tcctatgaca gtatttgctg
    13681 aagaagggac tgctgttcac tatagcaata aatttcaagg cagaaaaact gcaagtggtg
    13741 aagtctttga tcaaaagaaa ttaaccgctg cacacaaaca atatcctttt gggactaaag
    13801 ttagagtcac gaatttagaa aataaccgca gtgtaatcgt taaaatcaat gatcgcatgg
    13861 caactaaaaa tagcaatatc attgatgtga ccagtcgtgc agcaaatgag cttggtttta
    13921 ggaaaaaagg taaagctcaa gttagcctcg aagcagttga ttaataaaat agcacgaatc
    13981 agctggaaga gtcgaagcta acaagtaaat ttctattatc tttagtaatt gattaagccc
    14041 tatatcagca gattaagttt gctaatatag ggcttaagtt ttatatgacc ttacctaagc
    14101 aggtaaaagt aatatctatc aaatgaattt ataggaaaag tcgtaagtat cgtgatgaaa
    14161 catcagtgcc atatacccat tgattaataa ggcatcgtgc tgagtcgata atttcagtgg
    14221 cagtcatgcc gatcgggcca tgcagtgttt tgagtagagc atcggctgct gcaccatgta
    14281 aataaactgc aagtaataat gcattttctg gttttaaatt ttgagcaatc aaagcagcaa
    14341 tcataccgga caagatatca ccggtgcccg cactgcttaa accggggttt cctgtcgtat
    14401 taaaatagat atatccatta ggaaaaccac aaatactacc tgctcccttt aatacaacag
    14461 cacagttaaa tttttttact aagttttcag ttgcgttaat acggttcgct tgaacagttg
    14521 cagtatcgac ttggagtaaa cgcgcagctt cagccacgtg aggcgtgaga atcgtggcga
    14581 gttggcggct acagactagc tcctgaagct cagggtagcg tgcaattaaa tttaaagcat
    14641 ccgcatctaa tatgattgga atggatagct gtagtgcttt ttccaaataa atacaagctg
    14701 ctagctcagt tcctaaacca ggcccgacaa tcaagctgtt gatttggttt aactcaaaca
    14761 aatgatcgat ggaatgtagc attaattcag gttgctgcac atcgacgctt aaagcttctt
    14821 ttgcaatcaa gccaatataa actcgcccag ctcctaattt gagtgcggca gtgccagcaa
    14881 gtatcgctgc accgaccatt cctgctgttc ctccaatgac cccgacactt ccataagttc
    14941 ctttatgact attaatgagt ctaggagttg gtaagcaaga tttaataaac gaaaaattga
    15001 gtagccatga atgaggtgtt actaagattg tcgaattaag accgagattg caaagtacga
    15061 tttttccaga ataattacag ccatcatggg ttagtaatcc aggtttaagc ccgataaatg
    15121 tagccgttat cgttgcttta attgcggcac cggcgatatt accagtatct gcatcgagac
    15181 cactcggcac atcaagagaa agaacaggca gcttcatttg attaatggaa tgaatcaacg
    15241 cttgataatc gtcacacaaa ggccgcgtag atgtcagtcc tattccgaaa agaccatcga
    15301 taataatgtc ccattgctca ttggttggaa tacattgaac aatcgtacct tcaaccgcaa
    15361 tccatttttc aaaggcttgc tttgcttcaa cagaaagttt agattcattt cctgtgaaaa
    15421 ttactgttac tggatagccc caatttttga gatgtcgagc aacaaccaat gcatctccgc
    15481 cattatttcc cggtcccgct acaatcaaaa cacgttgcat cgtactcgtt agcatggctt
    15541 ctttcgcaag cgttgctgca gcaagacctg cttgttccat caacgatggt gtattaagct
    15601 gattgatcgc gatacgttca gcttcacgaa tttgtgaggt agaaaaaaca ggggtcgggt
    15661 tattcatttc aataatcgct tctcgtgtaa aattacgcgt ttaaccattc tcataattta
    15721 atattaatgc ttcagttccg cggtcgtcag gcgctttcct cttttcgttt agggaaatta
    15781 tctcatcaga ttcgttccat cgtatccgaa tttattaaga ttgaatcgga atattggtat
    15841 ttttgctcgc ttcagcaaaa tttaactcaa aacgagttaa taagattgca gcagttgtta
    15901 gatggtcacc tcattgagac acttcctcaa gttccgcatg gtttttttct agtattaccc
    15961 cgcctaggaa cggtttcacc ctggtcatcc aaagcgactg atatcgcgca tcactgtcaa
    16021 ttgcaaagta tcggacgtat cgaacgtggt gtcgcgttca atatcgaatt tcaatctcac
    16081 ataaccgatg agtctagaaa aagagttgaa gcattactgc atgatcgcat gacggagtct
    16141 attttttcct cattgcagga ggcatcaaaa ttgttccggc acatcgatcc tgggtcgctt
    16201 caaacaatcg atattcaaac cgcgggttca atggctttgc agcacgctaa ccaagcaatg
    16261 ggtttggcct tatcacccga tgagatcgat tatttagcag attatttctt aaagcaaagt
    16321 aagcgtaacc ccaccgatgt ggagctcatg atgtttgcgc aagcgaactc tgagcattgt
    16381 cgccataaaa tcttcaatgc agattgggca atcgatggcg ttcctcagac acaatcatta
    16441 ttctcgatga ttcgtcatac ccatcaacag actcctcagg ggacactgat tgcttattct
    16501 gataatgctg cagtgattga aggcgatatt atcgatcgct tctatcccgg taaatccaat
    16561 gtatatggtt atgaaaaaga attgactcat ctattgatga aagttgaaac ccataatcat
    16621 ccaacagcaa tttcaccttt tcctggggca gcaactgggg ttggtggaga gattcgcgat
    16681 gaaggggcta ccggcagagg cgcaaaaccg aaagcagggc taacgggttt ttctgtttca
    16741 aatcttcgaa tcccagattt tgttcaagcc tgggaagttg aatctgaatg gggtgaggcc
    16801 ggttatggta aacctgatcg gattgcatct gctttggaca tcatgttggc agggccaata
    16861 ggtggcgctg ctttcaataa tgaatttgga agacctaatt tagcaggcta tttccggaca
    16921 tttgaattgt caatgaatgg tgtaatgcga ggctaccaca agccgatcat gttagccggt
    16981 ggattaggcc aaattgccga taagcatacg actaagcaat ctttttcatc ggatgtgttg
    17041 ctcattcaac taggtggacc aggtttgcta attgggttag gtggcggcgc cgcttcaagc
    17101 atggatacag gcaccaatgc ggaagatctt gattttgatt cagtacaacg aggcaatcca
    17161 gaaatgcagc ggcgtgcgca ggaggtcatt gatcgttgtt ggcaattaga aaaaattggg
    17221 caggccaatc caattctttc tattcatgat gtgggagcag gtgggctttc aaatgcgcta
    17281 cctgaacttg tttatggtgc tggttgtggt ggaaagtttg atttatgtgc gattccatca
    17341 gaagaatcgg gtatgtcacc gatgcaaatt tggtgtaacg aggcacaaga acgttacgta
    17401 cttgcaatta agcgcgaatc attgagcttg tttcatgcga tttgtgaacg ggaacgctgt
    17461 ccctatgcag tggtaggaga gtcaaaacaa gtagatcagt tattactcac agattcacga
    17521 tcaacaatac cgccgattga tatgccgctt tccgtgctat taggtaagcc acctaaaatg
    17581 cagcgaactg tcgtgcatgt tgacaatcca ctcaaaccga tcgatttctc tcatatccaa
    17641 ttgaaagatg cgatttatcg aattctaaga ttaccgactg ttgcggataa atccttcttg
    17701 atcacgattg gtgatcgcag tattgggggt atgactgccc gtgaccaaat ggtaggtcca
    17761 tggcaaatcc cggttgccga tgttgcggtg acagcgatgg ggtatcaaac tcacttaggg
    17821 gaagcttttg ctatcggtga acgaactccc ctggcactca taaatgcagc cgcttctgct
    17881 cgaatggcga ttggcgaagc gctaactaat atcgctgctg cttcgatcga acgattggat
    17941 tatattaaac tgtcagccaa ttggatggct gctgccggtc atgcaaacga ggatgctgcg
    18001 ttgtttgatg cagttcatgc tgcagcgata gcattgtgtc ctcaactggg tatcagtatt
    18061 ccagttggaa aagattctat gtccatgaaa acgacttggt ggcaggaaaa taatgtgggt
    18121 gagaatcaga agaaagaagt gatagcaccc ttatcattga tcatttctgc ttttgcacgg
    18181 gtcaaggacg ttcgtcaaac actaacaccg cagctgcgca atgatctagg agaaacagcg
    18241 ctcattttaa tcgatttggg atttggaaaa aatcgaatgg gaggttctgc actagtccaa
    18301 gtttataatc aattgggcga tcaagcgcca gatctagaaa ctaaccctga aatagaaaaa
    18361 tttaagtcat tcttgggtgt gattcagcag ctcaaggaat caaatcagat cgtggcttat
    18421 catgatcgtt ccgatggtgg gctttttgta acattgtgtg aaatggtttt tgctgggcat
    18481 atcggggtta ccattaattt ggatcaactc tgctttgatc catcggcaag tgatcttgat
    18541 ggtgctgaaa taaagccaga agccctacaa ggtcgcttct ttgaacgctt actgagcgta
    18601 ttgttcaatg aagaattagg tgcggttatt caaattcagg atgtgcatca tcaagccgta
    18661 ttgcaagctt ttgctgaagt tgggttggct aaccattgtt ttgtaattgg aaaattgaat
    18721 caatctgatg agattcaaat tttacgcaac aacatcgccg ttattcgtga aaaacgtgtc
    18781 gatttgcatc gcgcatggtc agaaacgagt tatcacatgc aacgtatccg cgataatcca
    18841 gcgtgtgctc aacaagaata tgatcgtatt ttagatactg ctgatccagg gctgcattca
    18901 aagctaactt ttaatgtgca cgatgatatt gcatttccat cgattcaaat gcgatctcgc
    18961 cctaaaatcg ctattctgcg cgaacaaggt gtcaatgggc aggttgaaat ggctgctgct
    19021 tttgatcaag ctagtttttc agcagttgat gttcacatga gcgatattct tgctgggcgt
    19081 gatttattaa cagactataa aggcctagtc gcttgtggtg gcttttcata tggtgatgtt
    19141 ctaggcgcag ggaaaggttg ggcgaaatcg attttattta accccgttgc acgcgatgca
    19201 ttccaaacct tttttaaccg gcaagatacc tttgctttag gcgtttgcaa cggttgtcag
    19261 atgatgagca atctagaaga aatcatgcca ggtactgcgc actggcctcg ttttgagcgt
    19321 aatcaatctg aacagtttga agcacgcttt atattagttg aaatacccga aagcccttct
    19381 attttgttta aaggcatgga aggcagtcga ttaccgatta ctgtcgcgca tggtgaggga
    19441 tttgcttcgt ttcaaacctc gcagcaatta gcaacagcat ctcattatat tgctttgcgc
    19501 tatatcgatc attatggtaa tccaactgag cgatacccat ttaaccccaa tggttcggcc
    19561 ttaggtataa ctggcttaac ctcagtggat ggtcgattta ctattttaat gccacaccca
    19621 gaaagggtat ttcgtacaat tcagcattct tggcatgctg aagattggcc aacatattca
    19681 ccttggatga aaatgtttag gaatgcccgg caatgggtag ggtaaccgtt gaactaattg
    19741 cgtgttccgt gagtcatcct tattaactca tttacaataa ccctcaaaga aattaatcga
    19801 tgaccaataa taaaaataag cataagttac cacttaactt atcaatgcgc gtatggtttg
    19861 ccctcattgt tattttatct tcaaacgcat ccgctgaaat tatcccactc gtcaaaccca
    19921 ttcaaacttc acgagcagct tcatccacta aacaatgtgt tcctgtggca agttgggtga
    19981 tacctgggaa aaatgtaaag acttctttta ctgagattgc ttctcacgct aatcaacatt
    20041 ctgtcatttt actcggtgaa actcatgtta accgtgaaca tcatcgttgg cagctacaga
    20101 tgcttgctgc tttgcatacg atacgtcccg atatgatcat tggctttgag atgttccccc
    20161 gaagtgctca gaaggccctt gatcaatgga tcgctggtga attatcggaa tcggattttt
    20221 tacagaaaac aaactggaat tcagtttggg gaacggatgc aagcctttat ttgccgctgt
    20281 tccattttgc ccgcatgaat cgcataccta tgcgagcgct taatattgat agcgagctaa
    20341 ggaggcgagt ttctgagaaa ggttttgata gcgtaccgga gaatgagcgc gaaggagtta
    20401 ctagacctgc accaccaagt gctgcttatc ttgattttct tttaccgatt tatgagttac
    20461 atgatcgtaa gcataaacaa caagctaaat ctaataagta tgaccccgat tttttaaggt
    20521 ttgtagtcgg tcagcaatta tgggatcgtg cgatggccca agaaattaag gcagcgttgg
    20581 atttacttga aaaacgcaaa aattctctgg tagtgggtat catgggaaca gggcatattc
    20641 tgcatggttt tggtgtgcct catcaattaa aagatcttgg agtcaatggc ataaccactc
    20701 tgattccttg ggatacgaac aaatcctgta aacaattgac cgacggttat gccgatgcag
    20761 tgtttggatt aacaccccaa aatattgagg tgcctccttc ctttcaacgg ctaggtattc
    20821 gctatgaaat gatggtagag caaggagggg cccgtgttat ggaagttgaa aaagcgagta
    20881 ttgctgaatc ggcagggctg ctgacagggg atattatccg tgaaattgca ggggtcgcag
    20941 tgaaggatac aaatgatatc gttaccgtgg ttaaacgtca ggcaccgggt acatggctac
    21001 cgattagcat agaacgcgga aaagagctaa agcaaatcat tgcaaagttt ccggcgctag
    21061 cgaagtaacg cattggaatc attaatgaca ggtttaagag aaaaagagat taaatcaacc
    21121 ggaactaaat aataatttcc agttgattta atcaacctct acttaagagg aaaggataag
    21181 ggaagctatg aaaaacatac gctatgatat gcttgagtga agtaataaat caatgtgaca
    21241 tattgtcgcg taggcaagaa acgaaagtta atcgatgaca cacaatgaaa ctcagcgtaa
    21301 aaatggtttt aatggttttc ccccttcacg accgactgcc tcaatttcta gaaaaatttc
    21361 cttaatattc tttgcgctat tagaatctgg cttataacca acaggaataa aaagctgagg
    21421 atgatcgaaa ggtgcttttc gatagcggac acgctcatcg gtgagcgatc gtaaaaatgc
    21481 ttccaaatca gctttttcca tttccgtatt catgaggata ttcagcggaa tgattgtaga
    21541 g
//
LOCUS       DAOQKQ010000019        21431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_1062_length_21431_cov_6.258655, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000019 DAOQKQ010000000
VERSION     DAOQKQ010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 21431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21431
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1062_length_21431_cov_6.258655"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..1074
                     /locus_tag="PKZ49_02245"
     CDS             172..1074
                     /locus_tag="PKZ49_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HND35411.1"
                     /translation="MNPQLLPPAFPTSWANEWGEDEFGLYLDCNVNGIVQRFRWIVPG
                     TFTMGSPENEAEREEDETQHFVTLTQGFWLADSTCTQALWLAVMGNNPTFPIIIGENL
                     FDNLSDKVKATQQQLFSNDDLDPSLVHPVTRVSWDDVQKFIQTINAQLPDLQARLPTE
                     AEWEYACRAGTITPFSFGNNITPEQVNYHGEYSYAGAEKGLHRGRTVPVKSLPPNPWG
                     LYEMHGNVWEWCADRYTAYPSFAVIDQSGSATKEYRSLRGGSWASHAGRVRSAFRDGD
                     EANFNDGVIGFRLALGRKASTSQV"
     gene            1266..2108
                     /locus_tag="PKZ49_02250"
     CDS             1266..2108
                     /locus_tag="PKZ49_02250"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HND35412.1"
                     /translation="MSGIFISYRRHDSQSAAGRLADFLERSFGADQIFRDVETIEPGV
                     DFVQAIEKAIASCQVMLVVIGPRWLTITDQQGNRRLDKADDWIRIETKLALQRNIRVI
                     PVLVENAQPPTMDALPDEIKSLARREAHELSDKRWDYDVQCLIEALEKIPGIRRKVQT
                     TTFSQNAPVQPVTTRLTTKLGKVLAWIGGIFVALIMIGVFLDDTTTSITPPLSPPLTP
                     VTPQPLMQGLPSGSPTSTCGCWGYVEVGATRPNPQCASGIDTVVMCQGVCAGGGAPWQ
                     SLCQ"
     gene            2278..3138
                     /locus_tag="PKZ49_02255"
     CDS             2278..3138
                     /locus_tag="PKZ49_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HND35413.1"
                     /translation="MDKSALLSALAAEIEQGKMIFPTSTSAAINIKKMLDDPDCELDK
                     VTRLVQAEPLLATKVVAVANSAVFNRSGKRVTDVRSAISLLGFNMVRNLATAILTRQL
                     AGKAANSEVVTQLWKHSAYVASLAQVIARRVTKQNPDTAMFAGMIHEISWFYLLSREK
                     DFPGLTDGNFASSWVSDEDLDDETEIECEIKIGTAILKALAVPAPVVEGITCLWEGYL
                     AYPPTTLGDTLLFADQLSPIRSPFVLPSDETGEDIRAKMDTLTDQETLSEILRDSEEE
                     VKSLTEALGT"
     gene            complement(3203..3634)
                     /locus_tag="PKZ49_02260"
     CDS             complement(3203..3634)
                     /locus_tag="PKZ49_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35414.1"
                     /translation="MSFISVLLNAFLSYVYLAYYPAVLQFVFEPDAAMIITGIVLFVL
                     LLMLARRSSIRNSFGTNYRLRPGITGSLIGCFGLLACYVAVNIMMEQPLPGSPSGQLL
                     ATIVDQIGPWITAAVIAGIGLQFVLFGLRLLRDTFHSKIYH"
     gene            3949..5019
                     /gene="aroG"
                     /locus_tag="PKZ49_02265"
     CDS             3949..5019
                     /gene="aroG"
                     /locus_tag="PKZ49_02265"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HND35415.1"
                     /translation="MKYQTDDLRIVGMHELTPPVELHREYPVTEVATETVYTARQEIH
                     EILHGHDDRLLVITGPCSIHDVEAALEYAARLKHQREKFKQQLLIVMRVYFEKPRTTI
                     GWKGLINDPDLDNSFHINKGLRMARHLLLDLNRMGIPSATEYLDLISPQYLSDLIAWA
                     AIGARTTESQGHRELASGVSCPIGFKNGTYGNLNIAIDAIAAASRPHHFLSVTKEGTT
                     AIFATKGNEDCHIILRGGRKPNYDAASVTSAVEELQKAKLPARLMVDFSHANSHKDYR
                     RQVEVAENVAQQIAEGSHAIFGAMIESHLVEGNQKAEGKPREQLVYGQSITDACINFE
                     TTEQVLQQLAHAVDKRRKRRKR"
     gene            complement(5021..6277)
                     /locus_tag="PKZ49_02270"
     CDS             complement(5021..6277)
                     /locus_tag="PKZ49_02270"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HND35416.1"
                     /translation="MFLKVAGYSLLYRKFSVLLTVLSVTLSTFVLLGIEHIRTEARES
                     FGNTVSGVDLIVGARTGQLNLLLYSVFHIGHATNNISWASYQALLADQKLAWAIPISL
                     GDSHRGYRVVGTTGDFFRHYRYGEQQPLTFAAGHALVHRFDVVLGAQVAKNLGYRLGD
                     SIHLVHGIGQTQFSLHDERAFNIVGILKPTGTPIDHALYISLEGMEAIHLNWRDGVKL
                     PSHSSSDTEIDTRPLTPKAITAIFVGLQTKMATFQVQRHINDYRKEPLLAILPGIALT
                     DLWQITRTMENTLQLIAALVLLSSLLGLSTLLLSSIRERQREIAVLRAIGARPSFVFW
                     MIELEALLITLSGIALALIALVMATQSAQTVLAENYGLFITPFTLNPAALTVLGIIII
                     SALLLATIPALSAYRVSLHAGLALKQ"
     gene            complement(6267..6974)
                     /locus_tag="PKZ49_02275"
     CDS             complement(6267..6974)
                     /locus_tag="PKZ49_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HND35417.1"
                     /translation="MSSPAPPQAVMLRDLQFQYPGKATTYVLDIPFWSIAQGDRLFIQ
                     GPSGSGKSTLLNLIAGILIATCGKIEVLGQDLSELSPAQRDRFRAQHIGVVFQQFNLI
                     HYLSVIDNVRLAAYFANSNLAEVTTRAKHLFAALNLNESLFTQQADRLSVGQQQRVAI
                     ARALINQPEILIADEPTSALDQATRDRFIKTLLTLSSAQNTTLIFVSHDNTLAPHFQH
                     HINLAELNRAGEAQHVS"
     gene            7099..7719
                     /locus_tag="PKZ49_02280"
     CDS             7099..7719
                     /locus_tag="PKZ49_02280"
                     /inference="COORDINATES: protein motif:HMM:NF023164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HND35418.1"
                     /translation="MAILLLAVMLLLVACGKESSDKPNTSALTPSTAQQALDSSFKTI
                     EWIDLMPQDDLDALLNPPDYLDEIEDGSEEDQLSNQFQTAIAQASDDRYQQALVSTRI
                     KPEFDNQKIRLPGFIVPLEFGKNQTVTRFFLVPYFGACIHLPPPPPNQIVYGVFDKGL
                     KVDVLHEPFWINGVLHTTLMENDIAKAAYTMTVTEIVPYVDEEIAN"
     gene            complement(7716..8282)
                     /locus_tag="PKZ49_02285"
     CDS             complement(7716..8282)
                     /locus_tag="PKZ49_02285"
                     /inference="COORDINATES: protein motif:HMM:NF022439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2796 domain-containing protein"
                     /protein_id="HND35419.1"
                     /translation="MMKLSQFTFSQFLLGLLGWLFSGASLLAADAHHTHGAHVHGEAE
                     LYIIYEHSRLFIEFTSPAINLIGFEHPPQNEKQQNALSHAKQVLAEGKQLFQIESPHC
                     QLETATASAPYIDGTPSAEHFHPQGEHPDFHASYTFNCQANTKLTTIHTTLAAKFPGI
                     ERIKVLWIAYNQQGSTQLTANHATISLN"
     gene            8493..8765
                     /locus_tag="PKZ49_02290"
     CDS             8493..8765
                     /locus_tag="PKZ49_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014751147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HND35420.1"
                     /translation="MNKSDLIDAIAKSASISKASAGEALNGALAAIKEALKKNESVTL
                     VGFGTFKVGKRAARTGRNPRTGAEIKIKAAKVPKFAPGKALKDAVN"
     gene            8790..8865
                     /locus_tag="PKZ49_02295"
     tRNA            8790..8865
                     /locus_tag="PKZ49_02295"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8823..8825,aa:Val,seq:tac)
     gene            8901..8977
                     /locus_tag="PKZ49_02300"
     tRNA            8901..8977
                     /locus_tag="PKZ49_02300"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8935..8937,aa:Asp,seq:gtc)
     gene            9057..10943
                     /locus_tag="PKZ49_02305"
     CDS             9057..10943
                     /locus_tag="PKZ49_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HND35421.1"
                     /translation="MFDFVHRKKTIVQIILFLVMLPFLFWGIQSYRNESQSAVATVEG
                     EEIPRWEFDQAMRNQHDRLRNMLKDNFNSAMLDSPEMRLAVLEGLIQQKIMHREAEEI
                     GLTVLDSRLASEIQSISAFHDENGKFSYQRYEELLRRQQMTPVIFEDKVGAEILQQQL
                     LEPLSSSAMVADAIVQAVIRLSEVKREINQVEIAPDQYLSEVNPDEDAIKTYYDQHQA
                     EFLLPERVRIEYLVLSLNDLARQETVTSEEVRNYYEDHQSDYGQPEERLASHILIAVK
                     DTTSEEAHAEAKRKVEDILAQVTQDPDKFAELAKEFSDDSGSAKSSGELGFLGKGVLV
                     KEFEDALFQMEPNEIRGPVETAFGFHIIKLSEIKPAQVAPVEEVEDSIEEMLKRQKIT
                     SRFGEIAEEFSNIVYEQSDSLLPAADRFNLTVKQSDDWINKKSREPVEIANEKLMQAL
                     FSEDVIKEGRNTDAIEVMPDTFVSARVLEHKPATAQSLEVVRDQIVEKLKLQLAAEKT
                     VEEGSDKLARLIAGETDLVDWGDTPKTVSYMQSQGLDIETLRAIFQADVTQLPAYVGV
                     TNLQGKYNLVRINQVIEPDAADKEKLQSFSGQLKQMLLQEELAAYQASLRQRYSVKIR
                     PESY"
     gene            complement(10937..11722)
                     /locus_tag="PKZ49_02310"
     CDS             complement(10937..11722)
                     /locus_tag="PKZ49_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HND35422.1"
                     /translation="MGFLANKRILITGLLSNRSIAYGIAKAMQREGAELAFTYQGADL
                     RDRVAKLANEFNSTLLFSCDVQDDQQITQCFEDLGKHWDSLDGIVHSIAFAPREALTG
                     DYLESVNREAFHIAHDVSSYSFAALAKAGLKLMEGRNAALLTLSYLGAVRVMPSYNVM
                     GLAKASLEANVRFMASSLGKKGIRVNAISAGPIKTLAASGIGNFGKLLGYAEKVAPLR
                     KNVTTEEVGNVAAFLCSDLASGITGEITYVDAGFNTVAFDLDQ"
     gene            complement(11805..12908)
                     /locus_tag="PKZ49_02315"
     CDS             complement(11805..12908)
                     /locus_tag="PKZ49_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HND35423.1"
                     /translation="MSFHLFQINELYSNANGAIQFIELTIGNINGESFWQGQSIDVIQ
                     GNAVHSFRFTTNLPNPNTANKSVLIATQGFADLNFITPDYIVPDGFLFTSGSATVNFA
                     GADAVTYANLPINSVHSIDRNGTIQINSPKNFAGVTGTVPSNTILGTSGPDQLVGTNA
                     DNTIEAGDGNDRLNGLLGNDLLNGGTGIDTAIYSDNSSAYTINSTKDGISISGPEGSD
                     TLINMERLDFLDKNLAFDLAQGQSAGNTVRIIGAAFDANNIIPEYVAIGLQLFDSGQS
                     LLSVSELVVNSTLFQSMASSTNNEAFVNHVYRNVVGELPSTAERDLYVGMLKGSGGTL
                     TQAELLILAANAVVNETNINLVGLLQNGAEFSA"
     gene            complement(12960..13796)
                     /locus_tag="PKZ49_02320"
     CDS             complement(12960..13796)
                     /locus_tag="PKZ49_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018280564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HND35424.1"
                     /translation="MARPLRIEFPGSFYHVTSRGDRHEPIYLDNADRINWLALFGHVC
                     QRFNWICHTYCLMDNHYHIVVETIDGNLSKGMRQLNGVYTQAFNRRHDRVGHVYQGRY
                     KAILIEKDNYLLELSRYVVLNPVRAGIVKNVDQWPWSSYSAMIGKSDCPNWLQTKWVL
                     SQFSKQRKRAIAAYINFVHAGIGLPSIWNDLQGQIYLGKEAFIKKMQQHVQLDKNISE
                     IPRIQRSTQAKPLSYYSSFKERNTAIVAAYQTGNYTMKAIADEFGVHYATVSRIVKKA
                     EI"
     gene            13891..14337
                     /locus_tag="PKZ49_02325"
     CDS             13891..14337
                     /locus_tag="PKZ49_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HND35425.1"
                     /translation="MYQRILIPIDGSATSSCALKEAIKLAQEHSAQLALVNVYEDILY
                     WVDESYINYEELQETIRVNAEKLLAKGQTEVIQAGLPVETKLLEAKNDRIENVIIGEA
                     DRWHADLIVIGTHGRSGFSRLMLGSVAEGVARLTSVPVLLIHCAEK"
     gene            complement(14518..15291)
                     /locus_tag="PKZ49_02330"
     CDS             complement(14518..15291)
                     /locus_tag="PKZ49_02330"
                     /inference="COORDINATES: protein motif:HMM:NF016952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016603 - glutaminyl-peptide
                     cyclotransferase activity [Evidence IEA];
                     GO_process: GO:0017186 - peptidyl-pyroglutamic acid
                     biosynthetic process, using glutaminyl-peptide
                     cyclotransferase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HND35426.1"
                     /translation="MRKILIHCLESIGLKVLLSEIYFHLRTIGIKKVKPQIIRSLPHD
                     STAFTQGLAYYNHTLYESTGLVGHSRLRCIDPENGNLINEIEIDGEWCEGIAVVNNRL
                     IQLTYTSGRAIVYDVNSLKPYNEFSYEGEGWGLARCGNLFLMSNGTNRLFYRDENFKI
                     ISELPVWLNQRQLNQINDIECVGDKIFANVLFENCIYEIDNKTGRVRHIIDCREIVKN
                     SGRRNAQDVLNGIAYVPSTKTFYITGKCWPMLFEILIPE"
     gene            complement(15336..16517)
                     /locus_tag="PKZ49_02335"
     CDS             complement(15336..16517)
                     /locus_tag="PKZ49_02335"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HND35427.1"
                     /translation="MTPSNTETITWKLVSTSSQANIDSEAFKKKMLLDQDPRWLITFV
                     TGEDKQLARFVLEQSGAIIASATFFVHPSALRIAISDWTLFSIPVKRYTLFAEIWFDP
                     TITADKSLYLLALFAQLRKELSINEVIFLQAVVSDSALSDVLAQKSSLSTSYHVLNYG
                     EPYNHRYIDFGGDYNHYLSQLSSSTRSDLKRTQKRFVSAVNGQYKTICYQSPEEVGTF
                     LDAAMAVSALTYQYQLLGAGLRDRNILENRLLTTAKLGWFHSYVLFVDQKPVAFQVGH
                     LYQECYYAQEIGYDPDWAKLQVGIFLHTEIISNLLATPDLVKRFDFGNDDNTHKQRLS
                     NAQTTEKYVYLIPRHWRNNLFVFTMKITNRLSTSVGHLLNKYGLRKRVRQLLWKLGVM
                     K"
     gene            complement(16671..17873)
                     /locus_tag="PKZ49_02340"
     CDS             complement(16671..17873)
                     /locus_tag="PKZ49_02340"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HND35428.1"
                     /translation="MSNTTLEWIDLASLGSEVRQPVLNQIERLIQKHPDANIDHDPYW
                     LSAKCGNDKSSKLFVCITPDNTVAGYAPFFIHPSALSFQLFGISVYDYRIRRYCITAT
                     PLLAEEYQSLSSALLEKLLITLREIIGDRDSLFGLGVEKSSNFSQFILENTELRKYYQ
                     VLPYGQSYFRRLISLPKDFEAYLKGLGNSTRYEMRRALRLLEKDTEITTSYRIFTQPE
                     EVVELLQLLQQISLKTYQHQLLNLGINNNAENRHTFELAARKGWLRSLILFSNNEPIA
                     FQHGFLYNKCFYLTHIGYDPAWAKWSVGTITHAYLIQNLIEVGAEKFDFLYGDNGSKA
                     RLSNDQREEQNFYIVPRKFPLSFIASMLQAFNFVMNTLGKAIEKFGIKTLIRRFLRRR
                     ATSSSEKN"
     gene            complement(18283..19836)
                     /locus_tag="PKZ49_02345"
     CDS             complement(18283..19836)
                     /locus_tag="PKZ49_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HND35429.1"
                     /translation="MKKLVKQFDPFRLLALILWLCPTLALFLFGLIWLWQIDGFVYWL
                     IALILCSGAGYLLQQQLIRRDRQLLSEAVTEPNPDWPPSAEIAWQKVDQLANQCDPQA
                     WSIEDASWIVELGRQTLDTVSHCYHPDAEKPYLELTVPHTLLIIEQASRDLQRDITEN
                     IPFSDRFTIGDLLRLQRWKDKAEQAINVYRAGRALVNPMHALFGEIWQHLRQRSFSLA
                     RDELYRWFLRAYIRKVGYYAIDLYSGRRPIIDKTATVPTESPPTEPQQEKTRYPSEAS
                     HPPLRFLVLGRSNTGKSSLINALFGKLTTASDILPDTTRQFSSFAIEREGMEQALIFD
                     GPGCDSQLFDEQQMIIAALNADLIFWVTAANRPDRQDERRFLDHLRAEQLKRLNHRPP
                     SIIVVVSHIDLLRPASVWQPPYNLIDPTDTKAMNIRAAMQFIATDLAIPIEQIIPVCL
                     KPGREYNVQDTLWACILDQQDVAMRVRLLRCLDARKQTEDWALLRRQMISAGRFLWSL
                     PKSNEKRVI"
     gene            complement(19840..21054)
                     /locus_tag="PKZ49_02350"
     CDS             complement(19840..21054)
                     /locus_tag="PKZ49_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HND35430.1"
                     /translation="MKLSKITDWLTYWQKLQTILADPKIDEAALDASLRQVRQKIPLP
                     VFWLIGKTQSGKTSIIHALTGSEAAEIGNGLQPCTRFSSFYDFPAQMPVVRFLDTRGL
                     GEIDYDPSEDIHYCESQAHLLIAVMKVADLQQQIVLKVLQTVRTRHPDWPIILVQTGL
                     HELYGPHDQHLTPWPFDQDPLPNEVPTDLQRALVAQRQTAIALPGSAPMLWVPVDLTL
                     PEDGFSPTHYGLEPLWKAIELVLPLGLQRQLAGEKEIQDFFARTAHQHIVGYSLTAAG
                     LGALPAVDLVMVTTLQAKLLRDLAKLYGQKWNKQTTIEFFSLLGTAITSSYFVRMIGR
                     TLTKLIPGVGQTVGAVWGASASAATTYALGKAAVYFFTQRQNGLNINPELLRKAYAEA
                     LEASASLLKQRI"
BASE COUNT         5981 a         4431 c         5108 g         5911 t
ORIGIN      
        1 cccaataggg cccgttaccg gacaaggccg cgtgttgcgc ggcggcgcct ggggcgggcc
       61 tgacctccgt agcagcctct ttggcttccg gcttgcccta gggcataagc ctgccagtaa
      121 ggccggtaag gctggtgaga caggccgtca aggcaccgat agggaggcat gatgaatcca
      181 cagctactac cgcctgcctt tcctacttcc tgggccaatg aatggggtga ggatgaattc
      241 ggcttatacc tcgattgtaa tgttaacgga atcgtgcaac gctttcgctg gattgtgccg
      301 ggtacgttta caatgggttc acctgaaaac gaagcggaaa gagaagaaga tgagacacag
      361 cattttgtta ccttgacaca gggattctgg cttgccgata gcacttgcac tcaagcgtta
      421 tggctagctg ttatgggaaa taatcctact ttccctatta ttataggaga aaatttattc
      481 gataatttat cggataaagt gaaagcaaca cagcagcaat tattttctaa tgatgattta
      541 gatccttcac tcgtccatcc ggtaacacga gtgagctggg atgatgtaca aaaattcatc
      601 caaacgatta atgcacagtt accggatttg caggcaaggt taccgaccga agcggagtgg
      661 gaatatgcgt gccgtgcggg cacgattacg cccttctctt ttggcaacaa tattacaccc
      721 gaacaagtaa attatcatgg tgagtattct tatgctggcg ctgagaaggg gctacatcgt
      781 ggaagaactg taccggttaa atcattgccc cctaatccct gggggttata cgaaatgcac
      841 ggtaacgttt gggaatggtg tgcggatcgg tacaccgctt atccttcatt tgcagtgatc
      901 gatcaatcag ggtcagctac taaagaatat cgttcattgc gtggtggatc gtgggccagc
      961 catgctggta gggttagatc tgcttttcgt gatggagatg aagctaattt taacgatggt
     1021 gttattggtt tccggcttgc cctcggtcga aaggcgagca caagccaggt gtgagtcagc
     1081 ggcagccgag tggccgtagc ggagcaaggc ctagcgctgc cgcacagtat taaatcagct
     1141 attccgccat gaggatgtta gcgccaaaac aaagcattac aaaccgatgc catcgcattg
     1201 agatcgcata aaacctgcgt ataatgagga caggtaaccc atcgatacaa gtggagtgga
     1261 atagtatgtc aggcattttt atcagctacc gacgccatga tagccaaagc gcagccggta
     1321 ggttggctga ttttttagag cgcagctttg gggcggatca aatttttcgc gatgtcgaaa
     1381 caatcgaacc cggggtcgat tttgtacaag cgatcgaaaa ggcgattgcc tcttgccaag
     1441 tcatgctggt ggtgattggg ccacgctggc tcacgattac cgatcaacag ggcaatcgtc
     1501 gtttggataa agccgatgat tggattcgca tcgaaactaa gttggcgttg cagcgcaata
     1561 tccgcgtgat tccggtgtta gtggagaatg cgcaaccccc gaccatggac gcattacccg
     1621 atgaaatcaa atcgctggcg cggcgtgaag cgcatgaact cagcgataaa cgctgggatt
     1681 atgatgtgca gtgtttgatc gaagcgctgg aaaagattcc cggtattcgt cgcaaagtac
     1741 aaacgacgac cttttcacag aatgcaccgg tgcaaccggt taccactcgt ttgacgacga
     1801 aattgggtaa agtgctagcc tggattggtg gaattttcgt ggcgctgatt atgatcggcg
     1861 tattcttgga tgacacgacg acatcgataa ccccaccgtt atcgccgccg ctcacgccgg
     1921 ttacaccgca accgttgatg caaggattac cttccggttc gccaacttcg acgtgtgggt
     1981 gttggggtta tgtggaagtc ggcgcgacac gtcccaatcc gcagtgcgct tccggtatcg
     2041 atactgtggt gatgtgtcaa ggcgtttgtg cgggtggtgg cgcgccttgg caatcgttgt
     2101 gccaatagta ccttaaaggc gctactacgc ttttttgatc gcgctgaagc tgaataaccc
     2161 taccaaaagc cattttattt cagcctttta gcgcaacatt tttgcgtagt attatctcct
     2221 atgattgact ttagagagtg tcgtgctgga ttttgcaggc ggccgtagga gtgtgacatg
     2281 gataagagtg cattactcag cgcattggcg gctgaaattg agcaagggaa aatgattttt
     2341 ccaacttcga ccagtgcagc gatcaatatc aaaaaaatgc tggatgatcc cgactgcgag
     2401 ttggacaaag tgacacgatt ggtgcaagcc gaaccgttgc tagcaaccaa agtggtggca
     2461 gtggctaact cggcggtgtt caaccgctcc ggtaaacgtg tcaccgatgt acgttcagcg
     2521 attagcttgt tgggttttaa catggtacgc aatttggcaa ccgcgatttt gacacgtcag
     2581 cttgcgggta aggcagccaa tagcgaagtc gttacccagt tatggaagca ttcggcgtat
     2641 gtggcatcgc tggcgcaagt aatcgcgcgc cgcgttacga aacaaaaccc cgatactgcg
     2701 atgtttgccg gcatgattca tgaaatcagt tggttttatt tgttgtcgcg tgaaaaagat
     2761 tttccagggc taaccgatgg caattttgcg agttcttggg tgagcgatga agatttggat
     2821 gatgaaactg aaatcgagtg tgaaatcaaa atcggtactg cgatcctcaa agcgcttgcc
     2881 gtgccagcgc cggttgttga gggtattact tgcttgtggg aaggatattt ggcttatcca
     2941 ccgacgacgc tcggcgatac cttgctgttt gccgatcagt tatccccaat ccgttcaccg
     3001 tttgtattgc cgtcagatga aaccggcgag gacatcagag caaaaatgga tacactgacc
     3061 gatcaagaaa cgttgagcga gattctcaga gattcagaag aagaagttaa atcgcttacc
     3121 gaagcgttag gcacttagtt tttgattaca cgctgagcgc tgcctttaat cagcgcttca
     3181 acctgcaatc gtgttctttt cttcagtgat aaatcttgga atgaaacgtg tcgcgtagca
     3241 gtcgcaggcc aaacaataca aactgcaagc caatgcccgc aatgacggcg gcagttatcc
     3301 aaggaccgat ttgatcgacg atagtggcta ataattgccc actgggacta cccggtaacg
     3361 gttgctccat catgatattt accgcaacat agcacgcgag caagccaaaa caaccaatca
     3421 agctgccagt aatgcctggg cgtagtcgat agtttgtacc gaaggaatta cgaatcgagc
     3481 tgcgcctagc gagcatcagt aacagtacaa atagtacgat tcctgtaata atcatggcag
     3541 catcgggttc aaaaacgaat tgcagcaccg caggataata cgctaggtaa acataagaca
     3601 gaaatgcatt gagaagcaca ctaatgaagc tcataacaca cctcctgata ggaatggatt
     3661 gttagcatgg agtgccgtca tcgcatcgat cgccaggata gaggttgttt gatagccccg
     3721 ttatcatcgg atcgagggtg gtttgatgct agcaaaattc ccaaccgagg catgtgacaa
     3781 aaaacacacg aattcgacga taaattgtcc ttaagtttcc aaacttttgt tgagcgttat
     3841 caaaatttcc cttcgccctt ggaatcgcct ctaggttaaa ataggcactt tatttccaac
     3901 tgatcaatcg ctggttttta gccagtttgt ttaaacaagg gaaacaccat gaaataccag
     3961 accgatgatt tacgcattgt cggcatgcat gaattaacac cgcccgtgga gttacaccgg
     4021 gaatatcccg tgactgaggt cgcaactgaa accgtatata ccgcacggca agaaatccat
     4081 gagattttgc atggccacga tgatcgccta ttggtgatta cagggccttg ctcgatccat
     4141 gatgtagaag ctgcgctgga atacgcagca cgcttgaaac accagcgtga gaaattcaag
     4201 cagcaactgt tgatcgtaat gcgggtttat ttcgaaaagc cgcgtacgac catcggttgg
     4261 aaaggattga tcaatgatcc cgatttggac aatagttttc atatcaataa ggggctgcgg
     4321 atggcgcggc atttattgct cgatttgaat cgcatgggga ttccctctgc taccgaatat
     4381 ctggatttga tcagcccaca gtatttgtcg gatttgattg cttgggcggc gattggcgca
     4441 cgtaccacgg aaagccaagg acaccgtgag ctagcttctg gcgtttcctg ccccatcgga
     4501 ttcaagaatg gtacttatgg caatttgaat attgctatcg atgcaattgc agccgcgtcg
     4561 cgcccgcacc attttttgtc agtgaccaaa gaagggacca ccgcgatttt tgcgaccaag
     4621 ggtaatgagg attgtcacat cattttacgg ggtggtcgta agccaaatta tgatgctgcc
     4681 agcgtgacct ccgctgtcga agaattgcag aaagcaaagc taccggcgcg cttgatggtg
     4741 gattttagcc atgccaacag tcacaaagat tatcgtcgtc aagtggaagt cgctgagaac
     4801 gttgcccagc aaattgccga aggttcccat gcgatttttg gtgccatgat cgaaagccat
     4861 ttggtcgaag gaaaccaaaa agcagagggt aaaccgcgtg agcaattggt gtatggtcag
     4921 agcattaccg atgcttgcat caattttgaa accactgagc aagtattgca gcagttggcg
     4981 cacgcagtgg ataaacgtcg taagcgccgc aaacgttgat ttattgctta agggcgagcc
     5041 cagcgtgcaa tgaaacgcga taggcgctca aggcagggat ggttgccaat agcaaagcac
     5101 taataattat gataccgagc acggtgagtg cggcaggatt gagtgtgaaa ggggtgatga
     5161 atagaccgta attttcagcc agaaccgttt gcgccgattg ggttgccatt actaatgcaa
     5221 tgagcgcaag cgcgatgccg ctgagcgtaa tcaacagtgc ttccagttcg atcatccaaa
     5281 acacgaaaga cggccttgca ccaatggcac ggagcacggc gatttcacgt tgtcgctccc
     5341 ggatcgatga tagtagcagc gtgctcaagc ctagcagtga tgaaagcagc accagtgcag
     5401 caatgagttg tagggtgttt tccatggtgc gggtgatttg ccataaatcg gtgagggcaa
     5461 tgcctggcag aatggctagc agaggctcct tgcgataatc attgatgtgg cgttgcacct
     5521 gaaacgtggc cattttggtt tgcaggccga cgaagatggc ggtgatcgct ttcggggtga
     5581 gcgggcgagt atcgatctcg gtatccgatg agctgtgact gggaagcttg acgccatcgc
     5641 gccagtttaa atgaatcgcc tccatgcctt ccagactgat atataacgca tgatcgatgg
     5701 gggttcccgt tggtttcaga attcccacga tattaaaagc ccgctcatcg tgcagactga
     5761 actgagtctg cccaatacca tgcactaaat gaattgaatc accgaggcga taaccgaggt
     5821 tttttgcaac ctgtgcacct agcacgacat cgaatcgatg aaccagtgca tgacctgcag
     5881 caaatgttag cggttgttgc tcaccataac ggtaatgcct aaagaaatca ccggtagtac
     5941 ctaccacgcg atagccgcga tgagaatcac ccaaagaaat gggaatggcc caagcaagct
     6001 tttgatcggc gagtagtgct tgataactgg cccaggaaat attgttggtg gcatggccaa
     6061 tatggaatac tgaataaagc aataggttga gttggccggt gcgcgcgccg acgatcaaat
     6121 ccacaccgga gacggtgttg ccaaaacttt ctctagcttc agtgcggata tgttcaatgc
     6181 cgagcagtac aaacgtgctg agggtgacgg acagtaccgt caataatact gaaaatttac
     6241 gataaagcag gctatacccc gcaactttaa gaaacatgtt gagcctctcc ggcgcgattg
     6301 agttcagcca aattgatgtg atgctggaaa tgaggcgcaa gcgtgttatc gtggctgaca
     6361 aaaatcaatg tcgtgttttg tgcgctgctg agggtaagta atgttttaat gaaacgatcg
     6421 cgcgttgctt gatctagggc agaagtgggt tcatctgcga tcaatatttc aggttgattg
     6481 atcaatgcgc gggcaatggc aacccgttgt tgttgaccga cgctgagtcg atccgcttgt
     6541 tgtgtaaata atgattcatt aagattcaag gcggcaaata ggtgttttgc acgggttgta
     6601 acctcagcaa ggttgctatt cgcaaaataa gcggctaaac ggacgttatc gatcacgctc
     6661 aggtagtgaa tcagattgaa ctgttgaaag actacaccaa tatgttgagc acggaatcgg
     6721 tcgcgctgcg cgggtgacaa ttccgataag tcttggccga gtacttcgat tttaccgcac
     6781 gttgcgatca atatgccggc gatgagattg agtaatgtcg atttacctga gccggaaggg
     6841 ccctggatga agagtcgatc tccctgagca atcgaccaga aaggaatatc gagcacgtag
     6901 gttgttgctt ttccagggta ttggaattgc agatcgcgaa gcataacggc ttgaggtggc
     6961 gcaggggatg acacggaatc gactccttcc ttaacacatt ttccagtggg tttaattcgt
     7021 tcgattagca aggatgatat actggctgct caatattcaa aatgcttttt tatgaatcca
     7081 ggcttgcgga atagaaacat ggcgatttta ttgctcgctg tcatgctact gttggttgct
     7141 tgtgggaaag aatccagtga taaaccgaac acatcagcac ttacgccaag caccgcgcag
     7201 caagcccttg actcgtcgtt caaaactatt gagtggatcg atctgatgcc gcaggacgat
     7261 ctcgatgcat tattgaatcc gcccgattat ttggatgaga tcgaggatgg ttcggaagaa
     7321 gatcaactta gcaatcagtt tcaaactgcg attgcacagg ctagcgatga tcgttatcag
     7381 caagcattgg tatcgacgcg catcaagcct gaattcgata atcaaaaaat tcgtttgccg
     7441 ggttttattg tgccattgga atttggtaag aatcaaacgg taacgcggtt ttttttagtt
     7501 ccttattttg gagcctgtat tcatttaccg cccccgcccc cgaatcagat cgtttatggg
     7561 gttttcgata aaggattgaa agtcgatgtg ctacatgaac ccttctggat caacggagtg
     7621 ctgcacacca cgttgatgga aaacgatatt gctaaagcag cttataccat gactgttaca
     7681 gagatcgtgc cttatgtcga tgaggaaatc gctaattaat tgagcgaaat cgtcgcatga
     7741 tttgcggtta attgtgtcga tccttgttga ttgtatgcga tccatagcac tttaatgcgt
     7801 tcaatgcctg gaaattttgc tgctaacgtt gtatggatgg tggtaagttt tgtgttagct
     7861 tggcagttga atgtataact cgcatgaaaa tcagggtgtt ctccctgcgg atgaaaatgt
     7921 tcagcactgg gcgtaccatc gatataaggc gcgcttgcgg ttgcggtttc taattggcaa
     7981 tggggcgatt caatttgaaa taactgctta ccctcggcca gaacctgctt agcgtgagac
     8041 aaggcgttct gttgtttctc attttgagga gggtgctcaa agccgataag attgatggcg
     8101 ggggaagtga attcgatgaa taatctgcta tgctcataga tgatataaag ttcagcttct
     8161 ccatgaacat gcgcgccatg cgtgtgatgg gcgtcagcag ctaataggct agcgcccgaa
     8221 aacagccagc cgagtaaacc caataaaaac tgtgagaaag taaattggct tagtttcatc
     8281 attgcggtga ttgattaacg ataggttaat gatatgataa tttgagccta gattcaggtg
     8341 gttgttcaaa ttcttaccaa gaccactaag atttttaaga atttgttagg taacacttga
     8401 ccatgttcaa atggcttgat ataagggggt ttgcagaatc ttctgttaac gatttactac
     8461 taataaccca ataaaccgaa aaggggattt aaatgaataa atctgatctt attgatgcga
     8521 ttgcaaagtc tgccagtatt tcaaaagctt ctgcaggtga ggcgcttaac ggtgcattgg
     8581 ccgcgattaa agaagcattg aagaaaaatg aaagtgtaac attggttggt tttggaacat
     8641 ttaaagtagg aaaaagagct gcgcgaactg gccgcaatcc ccgtaccggt gccgagataa
     8701 aaattaaagc agcaaaagta ccaaaattcg cccctggtaa agcgcttaaa gatgcagtaa
     8761 actagcaacc tatatcgatt aagagaaaag ggtgcttagc tcagctggta gagcgtcgcc
     8821 cttacaaggc gaatgtcggc ggttcgatcc cgtcagcacc caccattaaa ggtggcttgc
     8881 caaatataaa attataagtt ggagtggtag ttcagttggt tagaataccg gcctgtcacg
     8941 ccgggggtcg cgggttcgag tcccgtccac tccgccattt tttcagtcag ctgtttaaag
     9001 ctgataagtg ttaacgctcc aatatacttc cattacttct taattaggtt ctagtcgtgt
     9061 ttgattttgt ccatcgcaaa aaaacgatcg ttcagattat cctcttttta gtcatgttgc
     9121 cctttttgtt ttggggcata cagtcgtatc gcaatgaaag tcagagtgcg gttgccacgg
     9181 ttgaaggtga agagattccc cgttgggaat ttgatcaagc gatgcgcaat caacatgatc
     9241 gcttacgtaa tatgctcaag gacaatttca atagcgccat gctggacagc ccagagatgc
     9301 gcttagccgt attggaagga ttgattcagc aaaagataat gcatcgcgag gctgaagaga
     9361 ttggacttac ggttttagat tctcggttag cgagtgaaat tcaatcgatt tctgcttttc
     9421 atgatgaaaa tgggaaattt tcttatcagc gttatgaaga gttattacgt cgtcagcaga
     9481 tgacgcctgt catttttgag gataaagtgg gtgctgaaat tttacaacag cagcttctgg
     9541 aaccactttc gagtagcgca atggttgctg atgcgattgt gcaggcagtt atccgtttga
     9601 gcgaagttaa acgtgaaatt aatcaagttg aaattgcgcc tgatcaatat ttgtccgagg
     9661 tgaacccgga tgaggatgcg ataaaaacct attatgatca acatcaagct gaatttttat
     9721 taccagaacg ggtgcgtatt gaatatttgg ttctgtcact taacgacttg gcgcggcaag
     9781 aaaccgtaac ttcagaagaa gttagaaatt attatgaaga tcatcaaagc gattacggtc
     9841 aacctgagga acggttagcg agccatatcc tgatcgcagt aaaagatacg acatcagaag
     9901 aagcacatgc ggaagctaaa cgtaaagtag aagatatttt ggctcaagtc acgcaagacc
     9961 ctgataaatt tgctgaactt gctaaggagt tttccgatga tagcggttct gcaaaatcga
    10021 gtggtgaact gggcttccta ggcaaaggtg tgttggtgaa agaatttgaa gatgcgttat
    10081 ttcaaatgga accaaatgaa attcgcggac cggttgaaac tgcttttggg tttcatatca
    10141 tcaagctttc tgaaataaaa ccagctcaag tcgctccagt tgaagaagtg gaagatagca
    10201 tcgaagaaat gttgaaacgc caaaaaatta ccagtcgatt tggtgaaatt gctgaggaat
    10261 tttctaatat cgtatatgag caaagcgata gcctattgcc tgcagcagat cgtttcaatt
    10321 taacggtgaa acagtcagat gattggatca ataaaaaatc gagagaacca gtagaaattg
    10381 cgaatgaaaa acttatgcaa gcgcttttct ctgaagatgt tatcaaagag ggacggaata
    10441 ccgatgcaat tgaggtgatg cccgatacct ttgtttctgc tagagtgctg gaacataaac
    10501 cagcaactgc gcaatcgttg gaagtggttc gggatcagat tgttgaaaag ctcaaacttc
    10561 agctagctgc tgagaaaact gtagaagaag ggagtgataa attggcccga ctcattgcgg
    10621 gagaaaccga tctcgttgat tggggtgata caccgaaaac cgtttcgtat atgcagtcac
    10681 aaggattgga tatcgaaacg ttacgtgcga tttttcaagc ggatgtaact cagctaccgg
    10741 cttatgtcgg tgttactaat ttgcaaggca aatacaattt agttcgtatt aatcaagtga
    10801 tcgaacctga tgctgccgat aaagaaaaat tgcagtcttt tagcggccaa ctcaagcaaa
    10861 tgttattgca ggaagagttg gcagcgtatc aggcaagttt gagacaacgt tattcagtaa
    10921 agatacgacc tgaaagctat tgatctaagt caaatgcgac agtattgaat cctgcgtcga
    10981 cataagtgat ctctccagta attccgcttg cgagatcact gcataaaaaa gctgcgacat
    11041 tacctacttc ttctgtcgtc acatttttgc gaagtggcgc aactttttcg gcatagccga
    11101 gcagttttcc aaaatttcca atacctgaag cagctaacgt tttgatcgga cccgctgaaa
    11161 tggcattaac gcggattcct ttcttaccaa ggcttgaggc catgaaacgg acattggctt
    11221 ctaggctagc tttggcaagc cccattacgt tataactcgg catgactctc acggcgccca
    11281 gatagctcaa tgtgaggagc gccgcatttc taccttccat tagctttaat cctgctttgg
    11341 caagtgctgc aaaactatat gaactgacgt catgcgcgat atggaaagcc tcacgattga
    11401 cgctttcgag gtaatcccct gttaatgctt ctcgcggtgc gaaggcaatt gagtgcacga
    11461 taccatcgag actatcccag tgtttaccga gatcttcaaa acattgtgtg atttgctgat
    11521 catcttgcac atcacaggaa aatagcagtg tactattaaa ttcgttagca agctttgcca
    11581 cacggtcgcg taagtctgca ccttggtagg tgaaagcaag ctcagcacct tctcgttgca
    11641 ttgccttggc gataccataa gcgatagagc gattgctgag taatcccgta attagtattc
    11701 gtttatttgc tagaaacccc atactcatac ctttttaatt gataatcgaa tcattatagc
    11761 gcaacgttcc tagaatgcga ggctctgcga aatcattgta gaacttaagc gctaaactca
    11821 gctccatttt gaagtaatcc aacaagattg atgttggttt cattgacgac tgcattggca
    11881 gcaagaatta ataattctgc ttgcgttaat gtgccgccac tgcctttaag cataccaaca
    11941 tagagatctc gctctgcagt agacggtaac tcgcctacta cattgcggta aacatggtta
    12001 acaaaagctt cgttattggt tgaactggcc atggactgaa aaagtgtgga attaacaacc
    12061 aattcactga cactgagtaa tgattggccg ctgtcaaata attgcaagcc aatggcgaca
    12121 tactcaggaa tgatattgtt agcatcaaaa gcagcaccaa tgatgcgcac ggtgttgccc
    12181 gctgattggc cctgtgcaag atcaaaagcc aaattcttgt cgagaaaatc gagccgttcc
    12241 atattgataa gggtatcgct accttcgggt cctgaaatgc taataccgtc tttcgttgaa
    12301 ttaatagtat atgcgctgct gttatcactg tagatagcgg tatcgattcc ggttccgccg
    12361 ttgagcagat cattaccgag tagcccgttt aatcgatcgt ttccatcgcc agcttcaatc
    12421 gtattgtctg cattagttcc aacaagctga tcgggtccac ttgttccaag aatagtatta
    12481 ctgggtacgg ttcccgtcac gcctgcaaaa tttttgggtg agttgatctg aatcgttcca
    12541 ttacgatcaa tggaatgaac gctattaatt ggcaggttag cataggtgac tgcatcagca
    12601 cctgcaaaat taacagtagc gctaccactc gtaaaaagaa atccgtcagg tacgatgtaa
    12661 tctggtgtga tgaagtttaa atctgcaaag ccttgagtgg caatgagtac cgatttattc
    12721 gctgtgttag gattaggtaa attggtggta aatcgaaaag aatggactgc attgccctgt
    12781 attacatcga tagattgacc ttgccaaaat gattcgccat tgatattacc aatcgtaagc
    12841 tcaataaatt gaatcgcgcc attagcattc gaataaagtt cattgatctg aaacaggtgg
    12901 aatgacatat tatctccttc taaaaagata tggggtcaga tcttgcatta atatagacac
    12961 tatatttctg cttttttaac aatacgactg actgtagcat aatgcacgcc aaattcatct
    13021 gcaattgctt tcatagtgta gttgcctgtt tgataggctg cgacgatcgc tgtatttctt
    13081 tctttaaacg atgaatagta tgaaagtggt ttggcttgag tacttctttg aatacgtgga
    13141 atttcactga tatttttatc aagctgaaca tgttgctgca ttttttttat aaatgcttcc
    13201 ttgccaagat aaatctgtcc ttgcaaatca ttccatatgc ttggtagtcc aatacccgcg
    13261 tgtacaaaat tgatataagc agcaatcgct cgcttacgtt gtttgctaaa ttgacttaat
    13321 acccatttcg tttgaagcca gttggggcaa tcgctttttc cgatcatggc agaatagctg
    13381 ctccagggcc attgatcgac attttttaca atacctgccc gcactggatt caatacaaca
    13441 taacgcgata attcaagcag atagttatcc ttttcaatga gaatggcttt atagcgtcct
    13501 tgatatacat gtccgacacg atcatgccta cgattaaagg cttgcgtata aacgccattt
    13561 aattgccgca ttcccttgga caaatttcca tcgatcgttt ctacaacgat atgatagtga
    13621 ttatccatca gacaatatgt gtggcaaatc cagttaaacc gttgacaaac atggccaaac
    13681 aaagcaagcc agttgattcg atcggcattg tcgagataaa tcggctcatg gcgatcacct
    13741 cgcgaagtta catgataaaa gctgcccgga aactcaattc ttaaaggtct ggccataact
    13801 atattaaaat tttattattg tgtagtagat tatatcgtta attattttat ctcaagacct
    13861 gaccccattt tgctttattg gagaaaagct atgtatcaac gaatccttat tccgattgat
    13921 gggagcgcta catcaagttg tgcgttaaaa gaagcgatca agttggcgca agaacattca
    13981 gcacagttgg cacttgtaaa tgtttatgaa gatattcttt attgggttga tgaaagttat
    14041 attaattatg aagaattgca ggaaacgatt cgggtaaatg cagaaaagct tctggctaag
    14101 gggcagacag aagttattca ggcggggttg cctgtcgaaa cgaaattgct tgaagcaaaa
    14161 aatgatcgaa ttgagaacgt gataatcggt gaagcagatc gttggcatgc tgacttgatt
    14221 gtgatcggca cgcatggacg ctcagggttt agtcgtttaa tgctaggaag tgttgcggaa
    14281 ggcgttgcaa gattaacgtc agttccggtg ttattaattc actgtgctga aaagtgactg
    14341 ataaaaatat tgataaagta caggctatgt gtagtagttg cgacgatcta tcgaatccct
    14401 cggatagata gtgtgtttga taataaaata ggagctttac cttagattac tggtaaattt
    14461 caatacaaaa ttctgggaga gtctcggaag atcatttatt tagttctacc agtcgcatca
    14521 ctctgggatg agaatctcga aaagcattgg ccagcatttt cccgtaatat aaaacgtttt
    14581 agtagaaggc acgtacgcga tcccattgag tacatcctgt gcattacgac gtccagaatt
    14641 ttttacaatc tcacgacaat caatgatatg acgcaccctt cctgttttat tgtcgatctc
    14701 gtagatacaa ttttcgaaaa gtacattcgc aaatatttta tcgccgacgc attcaatatc
    14761 gttgatctgg tttagttgac gctgattaag ccaaacaggg agttcagaga tgattttgaa
    14821 attctcatca cgataaaaca accgatttgt accattgctc atcaggaata aattaccgca
    14881 gcgcgccaag ccccaacctt caccctcata tgaaaattcg ttatagggct ttaagctgtt
    14941 aacatcataa acaatcgcgc gtccagaagt gtaagtaagt tgtattaaac gattattaac
    15001 aactgcgatg ccttcacacc attccccatc tatttcaatt tcattaatta ggttgccatt
    15061 ctcaggatca atgcatcgaa gtcgagaatg cccaactaaa ccagtgcttt cataaagcgt
    15121 atggttgtaa taagcaagtc cttgagtaaa agcggttgag tcatgcggca atgatcttat
    15181 gatttgaggt tttaccttct tgatacctat cgttcggaga tgaaaataga tttcactcag
    15241 gagcacttta agaccgatcg attctaagca atgaatcagt atttttcgca tcagttaaga
    15301 gaatcttgct aattatgtaa cgataatgat atttgctatt tcattacgcc taatttccac
    15361 agcagttgcc gaacgcgttt acgcaatcca tacttattaa gtaagtggcc tacacttgta
    15421 gataatcgat ttgttatttt catcgtaaat acaaagagat tatttcgcca atgacgaggg
    15481 ataagataaa catatttttc ggtcgtttgg gcattactga gtctttgttt gtgcgtatta
    15541 tcatcattcc caaaatcaaa acgcttaact aaatcagggg ttgcaagcaa attagagata
    15601 atttcggtat gtagaaaaat acctacttgt aattttgccc aatctggatc gtatccaatt
    15661 tcttgtgcgt aataacattc ttggtataag tgaccaactt gaaatgcaac tggtttttga
    15721 tcaacaaaca aaacatagct atgaaaccaa ccgagttttg ctgttgttaa aaggcgattt
    15781 tcaagaatat tgcggtcacg gagacctgcg cctaataact ggtattgata ggttagagcg
    15841 gatacagcca tcgctgcgtc taagaatgtg ccaacttctt caggtgattg atagcagatc
    15901 gttttatatt gaccattgac agccgaaacg aatctttttt gagtacgttt gagatcactt
    15961 cgtgtagacg agcttaattg gctcagatag tgattgtaat caccgccgaa atcaatatag
    16021 cgatgattgt acggttcccc gtaattaaga acgtgatagc tggtacttaa tgatgatttc
    16081 tgagctagca catcagataa tgcagaatca ctaaccacag cctgtaaaaa aattacttcg
    16141 ttgattgata gttcttttcg gagctgagca aataacgcta acaaatataa agatttatct
    16201 gcggtaatag ttggatcgaa ccaaatttca gcaaacagtg tatagcgttt tacgggtatg
    16261 ctaaatagtg tccaatcact aatcgcgatg cgaagtgccg aaggatgtac gaaaaaagta
    16321 gcactagcga ttattgcgcc agattgttca aggacaaatc ttgctaattg cttatcttca
    16381 ccagtgacaa aggttattaa ccagcgcgga tcctgatcaa gtaacatttt tttcttaaag
    16441 gcttctgaat caatattcgc ttgcgaacta gttgagacta acttccaagt tatcgtttct
    16501 gtatttgatg gagtcataac tacgtataga aatttattaa gttttaatgg taacgattaa
    16561 attaagttta aattttgttg agttttaatg atttaatctt attcgccaga aaacacatcc
    16621 aaactagcct tatccatttt tgatctagcg catctatttt taaatagaat ttaatttttt
    16681 tctgaagaag acgttgcgcg acgtcttagg aaacgacgaa ttagcgtttt gatgccgaat
    16741 ttttctatgg cctttcctaa cgtattcatc acaaaattaa aagcttgaag cattgatgca
    16801 atgaaagaaa gcggaaattt acggggaaca atatagaaat tctgttcttc acgctgatcg
    16861 ttacttagcc ttgctttgct tccattatca ccgtataaaa aatcaaattt ttcagcacca
    16921 acttcgatta aattttgaat taagtaagca tgtgtaattg tacccacaga ccacttagcc
    16981 caagctggat catagccaat atgcgttaga tagaagcatt tgttgtagag aaagccatgt
    17041 tgaaatgcga tgggctcatt gttgctaaaa agtatcaagc ttcttagcca accttttcta
    17101 gctgcgagct caaaggtgtg gcggttttcg gcattattgt tgatgcctaa attaaggagc
    17161 tgatgctgat atgtttttaa tgatatttgc tggagtagtt gcaaaagttc tactacttcc
    17221 tcgggttgag taaagatgcg ataggatgtg gtgatttccg tatctttttc caataaccgt
    17281 aaagcacgtc gcatttcgta acgagtacta ttacccagtc cttttaaata agcctcaaaa
    17341 tctttaggca aagaaatcaa acgcctaaag taggattgtc cataaggtag aacttgatag
    17401 tatttacgaa gctcggtatt ttctaatatg aactggctga aattagaaga cttttctaca
    17461 cctaagccaa acaaactatc acgatcccca ataatttcgc gtaatgttat aagtagtttt
    17521 tctaataaag ctgaggaaag ggattggtat tcttcagcta gtagcggggt tgcagtaata
    17581 cagtagcgac gaattctata gtcgtataca gaaattccaa atagctggaa gcttagtgca
    17641 gatggatgaa tgaaaaaagg tgcgtagcca gccactgtat tatcgggagt aatacatacg
    17701 aatagtttgc tagacttatc attcccacac tttgctgaga gccaataggg atcatggtct
    17761 atgttagcat cagggtgttt ttgtattaag cgttcaatct gatttaaaac aggctgacga
    17821 acttctgacc ccaatgacgc taaatcaatc cattctagag tggtattact caaaataatc
    17881 cttaaatttt gatttgctaa aacaatcttt caaggtagta attaatctcg ctgcaaaggt
    17941 acgatcagcc ggaatagatt attattgcat ttaaacaagc tcagccccac gattagtagt
    18001 attactgcaa gtatgcatta aattaacaag caagtaagat tagaataacc cagtttcatg
    18061 atagtaattc aagttaggct cagttcagag taatgcccaa ggcgctacgc taatacataa
    18121 ttattttaca tccttccagc ttgattgcta cgaaacttag gactaggtat tcttgtaagt
    18181 agtcggataa cacactgtga tatgttccca atatatcttg agattaccca ttgcgatatt
    18241 aacccatgac agtgattttc ccgctttgtt tggtgggaca agctatatca cgcgtttttc
    18301 gttgcttttg ggaagactcc ataagaatcg acctgcgcta atcatttgcc ggcgcagtaa
    18361 agcccaatct tcggtttgtt tgcgcgcatc aaggcaacgt agcaaccgaa cgcgcatagc
    18421 gacatcctgc tgatcaagaa tgcaggccca gagtgtgtct tgaacattgt attctctacc
    18481 cggtttaaga caaaccggga tgatttgttc gatcggaatg gccagatcgg ttgcgatgaa
    18541 ttgcatggct gctcgaatat tcatcgcttt ggtgtcagta gggtcgatta aattataagg
    18601 gggttgccaa acgcttgcgg gtcttaataa atcgatatga ctgactacaa cgataatcga
    18661 aggagggcga tgattgagtc gtttgagttg ttcagcacga agatgatcta ggaaacgtcg
    18721 ctcatcctgg cgatccggtc ggtttgccgc ggttacccaa aagatcaaat cggcattcag
    18781 cgcagcgatg atcatttgtt gctcatcaaa cagttggcta tcacaaccgg ggccatcgaa
    18841 aattagcgct tgctccatcc cttctcgttc tatagcgaag gaagaaaatt gacgcgtggt
    18901 gtcaggaagg atatcgcttg cagtggttaa ttttccgaat aatgcgttga tcagactgga
    18961 tttgccggta ttgcttctac cgagtacaag aaaacgcaaa ggagggtgtg aggcttcact
    19021 gggatatcgg gttttttctt gttgtggctc tgtgggagga gattcagtcg gcacagttgc
    19081 cgttttatcg ataatcggcc ggcgaccact atataaatcg atcgcgtagt agcctacttt
    19141 gcgaatataa gcgcgcaaaa accagcgata aagctcatcg cgtgccagac taaagctacg
    19201 ttgcctgaga tgttgccaga tttctccgaa cagcgcatgc atgggattca ccaatgctct
    19261 accggcgcga tagacattga tcgcctgttc ggctttatct ttccaacgtt gaagtcgcaa
    19321 taaatcacca atggtgaagc gatcactgaa agggatattt tctgtgatat cgcgctgtaa
    19381 atcgcggctc gcttgctcaa tgatcagtaa cgtatgcgga acggtcaatt cgaggtaggg
    19441 tttttctgcg tctgggtgat agcaatggga gaccgtgtct agcgtttgtc gccctaactc
    19501 cacgatccat gaagcatctt caatggacca agcttgagga tcgcattgat tggcgagctg
    19561 atcgactttc tgccaggcaa tttcagcgct tgggggccag tcgggattcg gttccgtgac
    19621 cgcttcgctt aatagctgtc ggtcgcgccg aatcaattgt tgctgcagca gataacctgc
    19681 gccactacat agaatcagtg cgatgagcca gtatacgaaa ccatcgattt gccatagcca
    19741 tatcaaaccg aataaaaata acgccaacgt agggcatagc cacagaatga gggctaataa
    19801 ccgaaagggg tcgaattgtt tgacgagttt tttcatgcgc taaattcgtt gtttaagaag
    19861 ggatgcgcta gcttcaagcg cctcagcata ggcttttcgc agcaattcag ggttaatgtt
    19921 taagccgttt tgtctctgcg taaagaaata caccgctgct ttacccagcg cgtaggtagt
    19981 cgcggcgctg gcactggctc cccaaaccgc acctaccgtt tgtccgacgc ctggaatcag
    20041 cttggttagc gtgcgcccta tcattctaac aaaatagctc gaggtgattg ccgttcccag
    20101 taggctaaaa aattcaatgg tcgtttgttt attccatttc tgcccataca gctttgctaa
    20161 atcacgaagt agcttggctt gcagtgtcgt gaccatcacc agatcgactg ctggcagtgc
    20221 gcctaatccg gcagccgtta gtgaatagcc cacaatatgt tgatgcgccg ttcgcgcaaa
    20281 aaaatcctga atttcttttt caccggcaag ttggcgctgt aatccaagcg gtagcactaa
    20341 ctcaatcgcc ttccatagcg gctccaatcc atagtgggtt ggactgaagc catcttccgg
    20401 tagcgttaga tcaacaggaa cccaaagcat gggtgcagaa ccgggtagtg ctatcgcagt
    20461 ttgacgttgt gcaaccaaag cacgttggag atcagtaggt acttcattgg gcaaagggtc
    20521 ctggtcaaat ggccatgggg taagatgttg gtcgtgtggc ccgtaaagtt catgtaaacc
    20581 tgtttgtacc aggatgatgg gccaatcggg gtggcgtgtt cgtaccgtct gaagcacctt
    20641 caacacgatc tgctgctgaa gatcagccac tttcatcacg gcaatgagaa ggtgggcttg
    20701 ggattcgcaa tagtgaatgt cttcgctggg gtcgtaatcg atttcaccca aaccgcgcgt
    20761 atccagaaac cgtaccacgg gcatttgtgc tgggaagtca taaaaactcg agaagcgcgt
    20821 gcatggttga agaccattgc cgatttcagc ggcttcactg ccagtgaggg catgaataat
    20881 tgatgttttt ccagattggg ttttaccgat aagccaaaat acgggaagcg gtattttttg
    20941 gcgtacttgc cttaatgaag cgtctaacgc tgcctcatcg attttggggt cggccaaaat
    21001 ggtttgcagt ttttgccaat aagtgagcca gtcagtgatt ttgcttaatt tcacaattgc
    21061 agtcttgaac gattaacgat gattattgta cagaacctcg ggcgtgtgtt agcaaagtta
    21121 gtctatttag gcagcaaaga catcacgaat catgggtggt agcgtaaatg ggttaaatcg
    21181 tcgggtgtga ataaattccc aagccgattt aacgccaact gatatggcgg tgttatacta
    21241 tctgacagta cggaattcgc gggttgatta cgattgcaag cagcaagatt tttcctcatg
    21301 gcagacacta ataatccatc accttcaaaa gcgccttcta atcagcaggt attcatcagc
    21361 tatagtcata gagaccgtga agcgtgtatt ctgctgcgtt ccatgttgga aacagcaggg
    21421 ttaagtgtat t
//
LOCUS       DAOQKQ010000020        21385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_1067_length_21385_cov_6.204923, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000020 DAOQKQ010000000
VERSION     DAOQKQ010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 21385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21385
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1067_length_21385_cov_6.204923"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..1306)
                     /locus_tag="PKZ49_02355"
     CDS             complement(74..1306)
                     /locus_tag="PKZ49_02355"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HND35431.1"
                     /translation="MALIVQKYGGTSVGSTERIKHVAQRVAKYHSQGHQVVVVVSAMS
                     GETNRLIALAKEIQSNPNPRELDVMVSTGEQVTVALLSMALMALNIKAKSYTGGQVRI
                     LTDSAHTKARILKIDEEKIRADLNAGSVVVVAGFQGVDEANNITTLGRGGSDTTGVAL
                     AAALKADECQIYTDVDGIYTTDPRIVPEARKLATITFEEMLEMASLGSKVLQIRSVEF
                     AGKYRVKLRVLSSFEEEGEGTLITFEEDNNMEQAVISGIAFNRDEAKITVLGVPDHPG
                     IAYQILGPIADANIDVDMIIQNVGHDGTTDFSFTVHRNELANAMAILKNSILPHIGAR
                     DVIGGDRIAKVSVVGVGMRSHAGIASRMFRVLAEEGINIQMISTSEIKISVVVDEKYM
                     ELAVRVLHKAFELDQVVQ"
     gene            complement(1457..3718)
                     /locus_tag="PKZ49_02360"
     CDS             complement(1457..3718)
                     /locus_tag="PKZ49_02360"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HND35432.1"
                     /translation="MMTSINLFLSTVSAEFRSYRDALRHDLTRPNVTVKVQEDFIVTG
                     TETLDMLDDYIRQCDAVIHLVGDMTGALAQAPSLAWIQSRYPDLAQRLPPLSPCLQPG
                     VPALSYTQWEAWLALYHRKRLIIACPEPNTPRDASYQMIDEQRAQQRQHLERLAAYER
                     YPLRFSSADRLVAEIWRSKLLDLLVHADSIQKSINPPPPKPIILPLASLGTLFKGRDA
                     LLEELQQSLSKAPSAADTPTVVRVLKGLGGVGKTRLALEYARQHVDDYSALLFIVGDS
                     AEALQRNLAALCGLLNLPARQEVDESKQCAAALTWLRQYPGWLLIIDNVDSESAALAV
                     ETLAPQLLGGHVLVTTRLAHWSHHLQPLLVDVLSSEAAVQFLLERTQTERRLLPNDAS
                     EAATLVDELGNLALALEQAGAYICQRRLTFEGYLQQWHRQRDAVLSWYNARQMHYPNS
                     VAVTWQTSFDQLSAPARQLLQCLAWLSPAPVPETLLEVPLADSDNAIDLFAALAELES
                     YSLVSRDDNPSFTVHRLVQEVTRRQQSDLENVHLSEALFWLNTAFSGDPQDVRDWPQL
                     NPLLPHAHAVAEYADLVGITTPTVRLFNQLGVMYLTKSLYDKAEPLMCRALEIDEKSF
                     GAYHPNVAVDLSNLAQLLQTTNRLSEAEPLMLRALKIDEKNFGADHPNVAIRLSNLAL
                     LLKATNRLSEAESLMRRAVIGFIRSLGTTHPNSQVVLNNYIVLLFDLGRTENEIETQL
                     HELMQWLQQNGSS"
     gene            3985..5139
                     /gene="ppk2"
                     /locus_tag="PKZ49_02365"
     CDS             3985..5139
                     /gene="ppk2"
                     /locus_tag="PKZ49_02365"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HND35433.1"
                     /translation="MVTAKKPTETAVVRKLTRPRRAASGDVGPDNSDKSFEDFAVKEG
                     IAAAQASKAVAVKDIIAHLSGKNTGALPQSLQAIFDGVSHDDALALRKALLELHGDAA
                     TKGRRNADDELADDWRDGGYPYKNLLSRKSYEKQKYHLQVELLKLQAWVKETGQRVII
                     LFEGRDAAGKGGTIKRFMEHLNPRGARVVALEKPSEVEQGQWYFQRYISHLPTAGEIV
                     LFDRSWYNRAGVERVMKFCTNDEYLEFMRQAPDFERNLVRSGIHLVKFWFSVSREEQR
                     RRFKEREMHPLKQWKLSSIDVASLDKWDDYTKAKEAMFFHTDTADSPWTVIKSDCKKR
                     ARLNAMRYVLHKLPYSNKKLESIGPLDPLIVGRAHIVYERGEKNNGIALL"
     gene            complement(5187..5393)
                     /locus_tag="PKZ49_02370"
     CDS             complement(5187..5393)
                     /locus_tag="PKZ49_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion lipoprotein TrbK"
                     /protein_id="HND35434.1"
                     /translation="MKFKSGILFLGVIGLNIALAGCSGPPTPSPANCSGRGLEVALSN
                     FRGDEVARQAYMDKCDEINAGADK"
     gene            complement(5550..7832)
                     /gene="metE"
                     /locus_tag="PKZ49_02375"
     CDS             complement(5550..7832)
                     /gene="metE"
                     /locus_tag="PKZ49_02375"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HND35435.1"
                     /translation="MITTHNLGFPRIGGKRELKFALEQYWSNRISESALLEIAAELRS
                     QHWQYQSKINWMPVGDFSLYDHVLDASFMVGNIPERAQSLAGNELDYYFRVARGRSNA
                     DIGASSCIHAAEMTKWFDTNYHYIVPEFNHATRFCLNAHRLFEQIQQAGKQFTQIKPV
                     ILGPVTYLWLGKSKDDSDKLNLLDDLIEVYAQLFQRLHQSGITWVQIDEPLLVMELPD
                     SWKHALRKTYFQLRATPLKLLLATYFGQLQDNLQLACELPVDGLHLDALSAQDEVAKF
                     VDWLPSHKILSLGIINGRNIWKTDLLDTLDFLEPIADRLQDRLWLAPSCSLLHVPVNL
                     EHEQKLDDEIRSWLSFAVQKLEELNLLAVALNQGRSAVAGELQKNKLAITARQQSDRV
                     NQASVKARMHSANDAMGMRQSPYQQRAVMQQNRFQLPLFPTTTIGSFPQTQSIRRIRQ
                     DFKAGKISDQSYVQAMQREIDCCIQEQEALGLDVLVHGEPERNDMVEYFGEQLNGFAF
                     TEFGWVQSYGSRCVKPPIIFGDVSRSKNITVDWIKYAQSLTKRLVKGMLTGPVTMLNW
                     SFVRDDQPRADTCLQLALAIRDEVLALEQSGIQMIQIDEAALREGLPLRKKQWNSYLD
                     WAIRAFRITANGVKDETQIHTHMCYSEFNDIMQAIAGMDADVITIETSRSSMELLEAF
                     RNFQYPNEIGPGVYDIHSPNIPSVDSIVALLQNAVQRIPKARLWVNPDCGLKTRSWEE
                     VKPALHNMVAATYFCRNMDG"
     gene            7935..8828
                     /locus_tag="PKZ49_02380"
     CDS             7935..8828
                     /locus_tag="PKZ49_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HND35436.1"
                     /translation="MIEHSHLTIIEALHTHGTLTEAANTLCLSQSALSHQIGYLEKKL
                     GVALWKREGRNLRLTQAGMLLLEVAQQILPVLAQAEKKLTAYREGRQGILRIGVECYP
                     CFEWLTGIIGAFMQSLPDIDVDIIQKFQFTGLEGLQNYHIDVLITPDLIKKENITYET
                     LTEYQLVLVVSIDHPLAGIEKLTPEQLSNEVLLTFPVPQERLDILTCFLTPAHSVPKM
                     LKPIESLEIMLQMTALGRGVCVLPEWLADIKMRSHRLKKIKIGNFGLNQKLYLALRES
                     DQAIPYIRKFIEIGRQSAHHR"
     gene            complement(8830..10173)
                     /locus_tag="PKZ49_02385"
     CDS             complement(8830..10173)
                     /locus_tag="PKZ49_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HND35437.1"
                     /translation="MKWERWLKVISLLSIFFIAGYPLISRADNLKSFLRSNRIEVGGW
                     IHGGATFNPSQSVGYNGPVIFADQANRFQLNQFNLFLQRSVPSEGKAWHFGGRFDFMF
                     GTDAIFTQAFGVPTFDVNSGEALKRSNWDLDLCCASTKTYGIALPQAYLEALVPVGNG
                     VNIKLGHFYTPTGFETVPAPDNFFYTRAYTLNAGEPFTHTGLLSNYTINPNWSILGGA
                     LTGSANGGWDGGWDKELGNWSGLAGFTWTSDNHATSFHFSGTYGNTSSRSNEVWAFYN
                     VVFKQKLGAKALLVLHHVLGHAGGVLLNNLKYTNVVRDADWMSAVAHLYYDLSENLSV
                     GIRGEWYRDEDGFRNPSPFRIAAATNIVDGTAKSYAGSLSAVTITPADYYDVTVGLSW
                     KAARSLQLKWDAIRKLVVRPNIRYDRVAAYHADTYRPFAGHKDQILFSLDFVLPF"
     gene            complement(10318..10689)
                     /locus_tag="PKZ49_02390"
     CDS             complement(10318..10689)
                     /locus_tag="PKZ49_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35438.1"
                     /translation="MKVINLLVLVVGYVLSASAFAETDLTNASHETLVTYHENIVKEA
                     ELKLAEHKQNLEDYEVHSYYYGRQGQDFQSHETANIEYYEEVITENLKAAELHRKMAS
                     EQNDSIKSANVDNSNVTRTFH"
     gene            11017..11922
                     /locus_tag="PKZ49_02395"
     CDS             11017..11922
                     /locus_tag="PKZ49_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35439.1"
                     /translation="MDQGNLSFFKLLFIASIILLLAACTTMQSISSTSEPQEPKIGFP
                     AKISLVSLAKEFGTIDETFKVENNECVVIDKEKPENSASLFNKLTDKLIKEGAVNRAD
                     LDEQQIKEAFNLVKSGEFKNDLNDTRDSLIKNIRVFQQSLRTKIKETVTNPQSAFDLI
                     RHSPEYLQINPSNNAQCEQGIKALLTHQNYDNLGTCAPGAVGKIIDKLKTDKTTSAII
                     ETAHIFTNKNNRSSRLAIIAYARMHGIKLTEDDLDKLDQFLDTTNESDLSPLIKVGID
                     KLAEKYGGKDIQAFIRKKDQECSLN"
     gene            complement(11942..12430)
                     /locus_tag="PKZ49_02400"
     CDS             complement(11942..12430)
                     /locus_tag="PKZ49_02400"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HND35440.1"
                     /translation="MDRIVVPQATWMRFAIDDAQRFMGLAEGGPFGACIVKGNEVIAV
                     AHSTVLREHDPTCHAEINAIRQAARALNQHHLNDCVIYSTTEPCPMCFSAIHWARIHT
                     IVFGTRIADVKTLGFNELDLSNEYMKREGHSQVEIVSEFLRDECEQILKTWQKMSHGI
                     AY"
     gene            complement(12506..13795)
                     /locus_tag="PKZ49_02405"
     CDS             complement(12506..13795)
                     /locus_tag="PKZ49_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HND35441.1"
                     /translation="MSKLIQAPVHRHEIFAWALFDFANSGYTTVVLTAIFNAYFVGVI
                     AQAYSSGTATLWWTITTAIANGVVLLTAPIIGAIADDRAWKKRFLMISASGCVGFTTL
                     LATAGPGDVSYAMALVILASICFFTGENLIAAFLPEIATPDKMGRISAFGWTIGYLGG
                     LLTLVLCLWVTLNTATQGAEMADAVPLTLLIVAAMFVVGSLPTFIWLRERVQPCERTA
                     INYCSIAYQRLHTTWQHARFHRDLFQFLIALTIYTCGINTVIVLAAVYAQEAMGFTTE
                     DNIQLILIVNISAALGAFVFGQIQDRLGSLRTLSMTLVIWICALLLAYFTYDRMMFWV
                     VANLVGIALGSSQSAGRAIIGLFSPPARAGEYFGLWGLATKLAAVIGPLCYGLITYVT
                     QGDHRTALLVTTCFFMVGLLSLIGIDEKRGRGSAIQD"
     gene            complement(13990..14622)
                     /locus_tag="PKZ49_02410"
     CDS             complement(13990..14622)
                     /locus_tag="PKZ49_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35442.1"
                     /translation="MKKRVWVIILILIGLTGCSTIYFLEGKEYEGRRQYIAARAEMYQ
                     RCIEGTISLNPPPIQRKLIALIPSQEAIFRTHFDNIKESNPNFPVTMEDLRQDPIYFG
                     MNENYRLVVELIKKRNLYQDLQIEEYETLTVPEVDEESDVFYIALPKDTKHRDMYYLN
                     TEKRGKLRVDYGPMDPDCETARAPFLALIPCEQFRDQLLTAIEILAKQSP"
     gene            complement(14636..15580)
                     /locus_tag="PKZ49_02415"
     CDS             complement(14636..15580)
                     /locus_tag="PKZ49_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nudix family hydrolase"
                     /protein_id="HND35443.1"
                     /translation="MIEVAAAVLVRPDGHFLLTSRPAGKPYSGYWEFPGGKIEKDESV
                     LQALARELYEELGIQIEQASPWITKIYAYQHATVRLHFYRVFTWHGELIGRENQQLSW
                     QTPDKVIVAPLLPANQPIIRSLSLPSVYGITQAAELGESTMLQKIELAAQQGLRLLQV
                     REKSMNKTQLKQFIDAIFKRTHPYQMKILINEDIALANAVGASGVHLTSSQLMEMSVR
                     PDLAWCGASCHNIEELYHAEQLELDFVVLGPVLPTKSHPDAITLGWRKFSSMIRDYPL
                     PVYALGGMIQADLPFAIEQGAHGIAMLRDIWKIEAVLK"
     gene            complement(15591..16487)
                     /locus_tag="PKZ49_02420"
     CDS             complement(15591..16487)
                     /locus_tag="PKZ49_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HND35444.1"
                     /translation="MSQFDLVLDRLNDLLIRVEALLPNQQDMTNFDAATAFRWRKQGH
                     NGYLQPVTHPHQIQLDALQGIDEQKQLIVQNTYQFVLGYPANNVLLTGARGTGKSSLV
                     KALLHQYADKGLRLIEVEKQDLIDLPDITEQIYQRPERFILYCDDLSFEMHEPGYKAL
                     KAILDGSIARASENILVYATSNRRHLMPESMKDNLDTRHIDGDIHPSESVEEKISLSE
                     RFGLWLSFYPFDQTQYLDIVRYWLAHYGIQHMNDAVRLEALRWALARGGRSGRIAWQF
                     ARDWSGKRKLAQSLRRKTRQPS"
     gene            complement(16494..17723)
                     /gene="argJ"
                     /locus_tag="PKZ49_02425"
     CDS             complement(16494..17723)
                     /gene="argJ"
                     /locus_tag="PKZ49_02425"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HND35445.1"
                     /translation="MPVNISPLLSEQLLPIAGISLGIAEAGIKRPNRKDLLTIVLDEN
                     VNVAGVFTQNRFCAAPVIVAKDNLTQASSIRALVINTGNANAGTGEQGIADARATCQH
                     LAKLINCHVAQILPFSTGVIMEPLPVEKIVTHLPQALADLNPDHWFKAAQAIMTTDIV
                     PKGVSRQIEIQGKTVTITGIAKGSGMIHPNMATMLGYIGTDACVAKAILQQMVRHVAD
                     HSFNCITVDGDTSTNDALILMATGRAGHSEITQLESQDYLKLQTAITAVARELAQMIV
                     RDGEGATKFITVCVEKGKNREECSRVAYAIAHSPLVKTAFFASDPNLGRILAAIGNSG
                     IIDLDVDLVQLYLDDVLVAEQGGRAQSYQEEAGQRVMKQAEITVRVVLNRGTMTATVW
                     TCDFSYDYVKINADYRS"
     gene            17812..18087
                     /locus_tag="PKZ49_02430"
     CDS             17812..18087
                     /locus_tag="PKZ49_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HND35446.1"
                     /translation="MKLTKTQPLLAKRENPFRADRELDVRGLVCPLPILRTKKALSEM
                     STGQVIKIVATDPGSVIDFQVFAEQTANDLLSFTESVDEFIFYLKKR"
     gene            complement(18116..19474)
                     /gene="pmbA"
                     /locus_tag="PKZ49_02435"
     CDS             complement(18116..19474)
                     /gene="pmbA"
                     /locus_tag="PKZ49_02435"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HND35447.1"
                     /translation="MNPDTSSGQNFSYSFAELQQVANDILNHAKRGGATACEANVSDG
                     FGLNVTVRQNAVETIEHNRDKGLSVTVYIGQKRGNASTSDFSPQAINDTVAAALSIAR
                     HTAEDNCAGLAEANLLATTFSDLDLYHPWNLPVEAAIELAQRCEAEALVVDKRITNSE
                     GASVSVGASQFVYANSLGFMGGYPVSRHSISCAVIASEADMMQRDYWFSVARDAHDLE
                     QIEAVGKKAGMRTVARLGAKKLSTCEVPVLFEAPLASSLIGHMVSAISGGSLYRKSSF
                     LLDSIGKQILPSDIQLHERPHIPKGLASCTFDEEGIATKDRSIIENGVVQGYFLNSYS
                     ARKLGLHSTGNAGGNHNLILSCAEPLGFEALLKKMNRGLLVTELLGHGVNGVTGDYSR
                     GAMGFWVENGEIGYPVEEITIAGNLKVMLSGIIGVGSDVIIRGSKQCGSILIDRMMVA
                     GL"
     gene            19606..20121
                     /gene="yjgA"
                     /locus_tag="PKZ49_02440"
     CDS             19606..20121
                     /gene="yjgA"
                     /locus_tag="PKZ49_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HND35448.1"
                     /translation="MIHDSHDDNESEEILSKTRRKKAMHALQGIGEQLVELDSQFIAE
                     LDLPEILVDAIREAKQLTKHEAKRRQMQYIGKLMRNIDAAPIQEKIDYWKNRDVNHTA
                     WLHQLENWRTRLLADENSINEFKQHYPETDIQHLRALIRNAHKETLSQKPPKSFRAIF
                     QELRTIIPEKK"
     gene            20178..20354
                     /locus_tag="PKZ49_02445"
     CDS             20178..20354
                     /locus_tag="PKZ49_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35449.1"
                     /translation="MIDDNSRFQMAVAKAWEDWLAVLEEDSFDSKLDGQEDLIDNLTL
                     SCPQIFQKDCLTLY"
     gene            20593..21306
                     /locus_tag="PKZ49_02450"
     CDS             20593..21306
                     /locus_tag="PKZ49_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35450.1"
                     /translation="MKKVLQNNWIAKGFVVIFMVLASFSLKAQLMLAHEGHHAGHGGC
                     EVKTGSFVVTVSIYEVPEGNIPPMHSYCNHVPKPGKVNVTIELPSDAREIPLAVRLVK
                     DDSHAGHGSGSPQSNATEKAASAVNEGDDHAHHHDETHGAGEFGLHYMPPMEHHSGII
                     VVAAELTELGQYAILLEQIDDKDDVRIVSKVPVHVGGDGGHAGHGSGIGLMEIVIGLM
                     LVVGGIGAFMFRKKKPQAS"
BASE COUNT         6225 a         4889 c         4432 g         5839 t
ORIGIN      
        1 atactccctt agcaggggag cgccttcgac cactcggcca tctctccgta tttcttagaa
       61 acgcgtaaac tgcttattgc acaacttgat caagttcaaa cgctttatga aggactctaa
      121 ctgctagctc catatatttt tcatcgacca cgaccgaaat tttaatttcg gaagtcgaga
      181 tcatttgaat gttgatgcct tcttcagcaa gtactcgaaa cattctactc gcaattccag
      241 catgagagcg cattcctaca cctacgaccg aaactttagc tattctatca ccaccaatca
      301 catctcgtgc gccaatatga ggcaagatac tatttttaag gattgccatt gcattggcta
      361 attcattacg atgaacagta aatgaaaaat ctgttgtgcc atcatgtcca acattctgga
      421 taatcatatc gacatcaata ttggcatcgg caatcggtcc caagatctgg tacgcaatgc
      481 ctgggtgatc cggcacacct aagactgtta tttttgcttc atcacgatta aatgcaattc
      541 ctgaaataac agcttgttcc atattattat cttcctcaaa cgtgattagc gtcccttcac
      601 cttcttcttc aaaactcgac aacactctca gcttaacacg atacttaccc gcaaattcta
      661 ctgagcggat ttgcaacact tttgaaccca aacttgccat ttccagcatt tcttcaaagg
      721 taatcgttgc tagtttcctc gcttcaggca caatgcgtgg atcggtggta tagattccat
      781 ctacatccgt gtagatctgg cattcatcag ccttaagtgc tgccgcaagc gcaactcctg
      841 tcgtatccga tccccctcta ccgagggtgg taatattatt ggcttcatct accccttgga
      901 aaccagccac cacgacaaca gagcctgcat tgagatcggc acggattttc tcttcgtcaa
      961 tcttcaaaat acgcgcttta gtatgggcgc tatcggtaag gattctcact tgcccacccg
     1021 tatagctctt cgctttgata ttgagcgcca tcagcgccat ggaaagcagt gcaacggtca
     1081 cttgctcccc cgtcgaaacc atgacatcca gttctctcgg gttagggtta ctttgaattt
     1141 ccttggctag ggcaattaaa cgatttgtct caccactcat cgcagaaacg actacgacaa
     1201 cttggtgccc ttgggaatgg tatttggcaa cccgctgtgc aacatgcttg atgcgctccg
     1261 tgctgcctac cgacgtgccc ccatattttt gaacaattaa cgccacttaa aaataccgat
     1321 cagaattttg aataacaaaa atgttttagc tttaataccg tcgactcatt aactcagcga
     1381 ttttacacat ttcttgaaaa gaagactatg ctcattttac gattgaatta cacaaggcaa
     1441 ctaacgataa acttgcttaa gacgagccgt tttgctgcaa ccactgcata agctcatgga
     1501 gctgcgtttc aatttcgttt tccgttctac ctaaatcgaa aagtagcaca atatagttgt
     1561 tcagcacgac ctgcgagttg ggatgcgtcg tgcccaaact ccggataaat ccaatcactg
     1621 cacggcgcat caacgactcc gcctcactta aacgattggt tgctttaagt agtaatgcca
     1681 ggttgctgag gcgaatggcc acattgggat gatccgcccc aaagtttttc tcatcgatct
     1741 ttaaggcacg gagcatcaat ggttccgctt cgcttaagcg gttggttgtc tgaagcagtt
     1801 gcgccaggtt actaagatca acggcaacat tagggtggta cgctccaaag cttttctcat
     1861 cgatttccag cgcgcggcac atcaacggtt ccgccttatc atataaggat ttagttaaat
     1921 acatcacccc caactgattg aacaatcgaa ccgtgggggt agttattccc actaaatccg
     1981 catactcagc gacagcatgt gcatggggca acagcggatt caactgtggc caatcgcgca
     2041 catcttgcgg atcaccgcta aacgcagtat tcagccaaaa caaggcttcg ctcagatgca
     2101 cattttccaa gtcgctttgc tggcgacgcg tgacttcttg cactaagcga tgcacggtaa
     2161 aactgggatt atcatcacgt gacactaatg aataggattc cagctcggct aatgctgcaa
     2221 acaagtcaat tgcgttgtcg ctatcggcta gcggcacttc gagcaaggtt tccggaaccg
     2281 gcgcagggct gagccaggct aagcattgta ataactgccg ggcgggagcg ctgagctgat
     2341 cgaacgaggt ttgccaggtt accgcgacac tgttcggata gtgcatttgc cgcgcattgt
     2401 accaagacaa taccgcatca cgctggcgat gccattgctg aagatagccc tcgaaggtta
     2461 ggcggcgctg gcagatataa gcgccagctt gttccagcgc caatgcgagg ttgcccaatt
     2521 catcgaccag agtggctgcc tcgctagcat cattgggcaa caaacggcgc tcggtttggg
     2581 tgcgctccag caggaattgg acagccgcct cacttgataa aacatctact agcaatggtt
     2641 gcaagtgatg actccaatgt gccaagcggg tggtcaccag cacatgaccg cccaacaact
     2701 gtggcgccaa tgtctcaaca gctagcgcag cagattcgct atcaacatta tcgataatca
     2761 gcaaccagcc gggatactgg cgtaaccatg tgagagcagc agcacattgc ttgctctcgt
     2821 caacttcctg tcgcgctggc aaattcagta atccacataa tgccgccagg ttgcgttgca
     2881 gcgcttctgc gctatcaccc actataaaca ataacgcact atagtcatcg acatgctgtc
     2941 gggcgtattc caacgctaat cgagtttttc cgaccccacc gaggcccttc aacacacgta
     3001 ccacagtcgg tgtatcagca gcgctaggtg ccttgctcaa actttgctgc agctcttcca
     3061 ataacgcatc gcgaccttta aacagcgtac cgagacttgc gagtggaaga ataataggct
     3121 ttgggggtgg aggattaata gacttttgaa ttgagtcagc atggacaaga agatcgagta
     3181 gttttgaacg ccatatctcg gctaccaatc ggtcagcact ggaaaacctt agcgggtaac
     3241 gctcgtaagc agctaggcgc tcgaggtgtt gccgctgttg cgcgcgctgt tcgtcgatca
     3301 tctgatacga agcatcgcgc ggtgtattcg gttctgggca ggcgataatt aatcgtttgc
     3361 gatgatacaa cgctagccag gcttcccact gagtgtagga tagcgcgggt accccgggtt
     3421 gcaaacaagg gctcagcggt ggcaagcgtt gcgcaagatc gggataacgc gattgaatcc
     3481 atgccaacga cggcgcttgc gccaaagccc ccgtcatgtc gccgactaga tggatcactg
     3541 catcgcattg gcgaatgtag tcgtcgagca tgtccaatgt ctcggtgcca gttacgataa
     3601 aatcttcctg cactttgacg gtaacgttag ggcgggtaag atcgtgtcgc aacgcatcgc
     3661 gatagctgcg aaattctgca ctcacggtgg ataaaaagag attaatggat gtcatcattg
     3721 tttttgttgc gatttatggg cagcttacca ttcagtaagt tactatttgc caaaacacca
     3781 tgcgatcata cctctgtatt tttacgctcg gatgccataa taatttcaaa ttcgctggaa
     3841 tcacaaatat ttggcttcaa ctccctgaat ttaacaaaat gacacgtctt ttcttgtttt
     3901 ttatgcgtat aaaaatctta taatgattac aagcaatcaa aagcgatgtt tagctgcata
     3961 ccatccgaaa taaggagatt gaacatggta actgcaaaaa aaccaactga aacggcggtc
     4021 gtcagaaaat taacgcgtcc acgtcgcgct gcatcaggtg atgtaggccc tgataatagc
     4081 gataaaagct ttgaagattt cgctgtcaag gaaggaattg cggcagcgca agcatccaag
     4141 gcggttgcgg ttaaagatat catcgcgcat ctttcgggaa aaaataccgg ggcgttaccc
     4201 caatccctgc aagccatctt cgatggcgtc tcacatgatg acgcgttagc gttacgcaag
     4261 gctttgctag agttacatgg ggatgccgca accaaaggcc gcagaaatgc cgatgacgaa
     4321 ctcgcagatg attggcgaga tggcggttat ccctataaga atctcctttc tagaaaatcc
     4381 tatgagaagc agaaatatca tctgcaagtt gaactgctca agctacaggc gtgggtcaag
     4441 gaaaccgggc agcgcgtcat tattcttttc gaggggcgtg atgctgcagg aaaaggagga
     4501 acgattaaac gtttcatgga acacctcaat ccgcgggggg cacgcgtggt tgcattggaa
     4561 aaacccagtg aagtagagca agggcagtgg tatttccaac gctatatttc gcatttaccc
     4621 accgccgggg agatcgttct gtttgatcgc tcttggtata accgtgcagg ggtagagcgt
     4681 gtcatgaagt tttgcactaa cgatgagtat ttagaattca tgcggcaagc acctgatttt
     4741 gagcgtaatc ttgtgcgaag tggcatccac ctcgttaaat tttggttctc ggtaagtcgg
     4801 gaggaacaac ggcgtcgatt caaggaacgt gaaatgcacc ccctcaagca atggaagctc
     4861 agttcaatcg atgtagcgtc gctcgacaaa tgggatgatt atacgaaagc gaaagaagcc
     4921 atgttttttc atacggatac cgctgattca ccctggacag tcatcaaatc cgactgcaag
     4981 aagcgagcgc gtttgaatgc gatgcgctat gtattacaca aacttccgta ttcaaataag
     5041 aaacttgaat cgattggtcc actcgatcca ctgattgtgg gtcgggctca tatcgtctat
     5101 gagcgcggtg agaaaaataa cggtattgcg ttattgtgat agatatccag ctacgctcag
     5161 gtagctggag taggcagact atttaattat ttatctgcac ctgcattgat ttcgtcacat
     5221 ttatccatat aagcttgccg tgctacttca tcaccacgga aattagataa cgcaacttcc
     5281 aggcctcttc ctgaacaatt cgctggactt ggtgtcggtg ggccgctgca ccccgctaat
     5341 gcgatattca aaccaatcac acccaaaaat aaaattcctg atttaaactt cattattatt
     5401 tcctcccaat gagatacaca acacaatctg atgacttaat cagattatgc ctacttttgt
     5461 cgatcacgcg gatctaacat tagaattcta cctgaatctg cttgattaca gcagtaggca
     5521 tgagaaataa gttttgacta ccggataaat cagccgtcca tattcctgca aaaataagta
     5581 gcagcaacca tattatgtaa tgctggctta acttcttccc aactgcgcgt ctttaatcca
     5641 cagtctggat taacccataa ccgcgctttg ggaatccgct gcaccgcatt ttgcaataaa
     5701 gctacaatcg aatcgactga cggtatattc ggcgaatgga tatcataaac gccaggccca
     5761 atctcgtttg gatactgaaa atttctaaac gcttcgagta actccatgct ggaccgagac
     5821 gtttcaatgg taataacatc tgcgtccata cccgcaatcg cttgcatgat atcgttgaat
     5881 tccgagtagc acatgtgcgt atgaatttga gtttcgtctt tgacgccatt tgcagttatc
     5941 cgaaaagcgc gaatcgccca atctagatag ctattccatt gtttcttgcg gaggggtaac
     6001 ccttcacgca atgctgcttc atcgatttgt atcatttgaa tgcctgattg ctccagcgct
     6061 aatacttcat cgcgtatcgc taacgccaat tgtaagcagg tgtcagccct tggctgatca
     6121 tcgcgcacaa acgaccagtt cagcatcgtg acaggaccgg ttaacatgcc tttgacaagt
     6181 ctttttgtta atgactgcgc gtacttaatc caatctaccg ttatattttt tgagcgtgat
     6241 acatcaccaa aaataatcgg tggtttaacg caacgtgagc cataagactg tacccaacca
     6301 aactctgtga atgcaaatcc attcaattgc tcaccaaagt attcaaccat gtcgttgcgc
     6361 tccggttcgc catgaactaa cacatccagc ccaagcgcct cttgttcttg gatacagcag
     6421 tcaatttcgc gttgcatcgc ttgaacatag gattggtcag atattttccc agccttgaaa
     6481 tcttgccgaa ttctccgaat cgattgggtt tgcgggaaag agccaatcgt tgtcgttgga
     6541 aacaagggta actggaatct gttttgctgc atcactgctc tctgctgata cggtgattgg
     6601 cgcatgccca tggcgtcgtt agcggagtgc atacgcgctt taaccgaagc ttgattaaca
     6661 cggtcagatt gctgacgggc tgtaattgcc aacttatttt tctgtaactc acctgccaca
     6721 gcacttctac cctggtttag ggctaccgct aatagattta attcttccag tttctgtact
     6781 gcaaatgata gccacgatcg aatttcatcg tctaattttt gctcatgttc cagattaaca
     6841 ggcacatgaa gtaatgaaca ggacggcgca agccataaac ggtcttgtaa gcgatcagca
     6901 ataggttcca gaaaatctaa tgtatccagt aaatcagttt tccagatatt gcgtccatta
     6961 atgatgccta atgagaggat tttatggcta ggcaaccaat caacaaattt ggcaacttcg
     7021 tcttgagcgg acaaagcgtc caaatgcaag ccatcgaccg gtaactcaca ggcaagctgt
     7081 aaattatctt gcagttggcc aaaataagtt gcgagcagca gcttaagcgg ggttgcacgt
     7141 aattgaaagt aggtttttct gagggcatgt ttccatgaat ctggcaactc catcacgagc
     7201 agcggttcat cgatttgtac ccaagtaatc cctgattgat gaagtcgttg aaataattgc
     7261 gcgtagacct cgatcaaatc atcgagcaag tttaacttgt cactatcatc tttagacttc
     7321 ccaagccaca agtatgtaac aggccctaga atgaccggtt tgatttgtgt aaattgcttg
     7381 cctgcttgct gaatttgttc gaataatcga tgggcattca agcagaaccg cgtagcatga
     7441 ttaaactctg gcacgatgta gtggtagttt gtatcaaacc atttcgtcat ctcggctgcg
     7501 tggatacaag atgatgcccc aatatccgca tttgaacgtc ctcgagctac ccggaaataa
     7561 taatctagct cgttaccggc caacgactgc gctcgttcag ggatattgcc caccataaaa
     7621 ctcgcatcca atacatgatc gtataatgaa aaatcgccaa cgggcatcca attgattttg
     7681 gattgatatt gccaatgctg acttctcaat tccgctgcga tttccagcaa cgctgattca
     7741 ctgatcctgt tactccagta ctgttctaag gcaaatttca attcacgctt accaccaata
     7801 cgcgggaatc ccagattatg tgttgtaatc atcttttatc actcaaagta aagaataaag
     7861 ttaagtcact tattctagga gtgataatct atgaaaaata atgatatatt ttatagattc
     7921 aattagttta actcatgata gagcatagcc atttaaccat tatcgaagcg ctgcatacgc
     7981 atggaacgct tacggaagcc gccaatacac tttgcttaag tcaatccgcc ttatcacatc
     8041 agattggcta tctggaaaaa aaattggggg tagcgctatg gaaacgcgaa gggcgcaatt
     8101 taagactgac ccaggcggga atgctattgc tagaagtcgc tcagcaaatc ttacccgtgt
     8161 tggcacaagc agaaaaaaaa ttgactgcgt accgagaggg tcggcagggt attttacgga
     8221 ttggtgtgga atgttatccg tgcttcgaat ggctcacggg tatcattggt gcatttatgc
     8281 aatcattgcc cgatattgat gtcgatatca ttcaaaaatt tcaattcaca ggcttggagg
     8341 gcttacaaaa ttatcacatc gatgtactca tcacgcccga cttgatcaag aaggaaaata
     8401 ttacctacga aacgttgact gagtatcaac tggttctggt tgtttctatt gatcacccat
     8461 tggctggaat tgagaagctg acacccgaac aattatccaa tgaagttttg ttgacattcc
     8521 ccgtaccaca ggaacgactg gatatcttga cgtgttttct aacgccagca cacagcgtac
     8581 cgaaaatgct aaaacccatt gaatcactgg aaatcatgct gcaaatgacc gcattaggac
     8641 gtggtgtatg cgtgctaccc gagtggcttg ctgatattaa aatgcgatct catcgcctta
     8701 aaaaaatcaa aatcggaaat tttgggctca atcagaaatt atatctggcg ttacgggaat
     8761 ccgatcaagc tattccctat attcggaaat tcattgagat tggccggcaa tcggctcatc
     8821 accgctaaac taaaaaggta aaacaaaatc caatgagaat agaatttggt ctttatgtcc
     8881 agcaaagggg cgataagtat ctgcatgata tgcggcaact cgatcgtatc tgatattagg
     8941 ccgcaccacc aattttctaa tcgcatccca ttttaactgt aaggatcttg cagctttcca
     9001 gcttaatcct acggtaacat cataataatc agccggtgta atggtaactg cacttaaact
     9061 tcctgcataa cttttggcgg taccatcaac gatattagtc gctgccgcaa ttctaaaagg
     9121 ggatggattt cggaaaccgt cttcgtcacg gtaccattct ccccttatcc caaccgataa
     9181 attttctgat aagtcatagt ataaatgcgc aactgcactc atccagtccg catcacgcac
     9241 cacgttagta tattttaaat tgttcaataa caccccccct gcatgcccga gcacatggtg
     9301 taatactaat agtgctttag cacctaattt ttgtttaaat accacattgt agaaagccca
     9361 tacctcatta gatctagagg aggtattgcc ataagtcccc gaaaaatgga atgaagttgc
     9421 atgattgtcg ctcgtccaag tgaaaccagc tagaccgctc cagtttccta attctttgtc
     9481 ccaaccgcca tcccaaccac cattagcgct gcctgtaagc gcgccaccca gaatggacca
     9541 gtttggattg attgtgtaat tggataataa cccggtatgg gtaaacggtt cgcccgcgtt
     9601 aagcgtataa gcacgcgtat agaaaaaatt atctggtgct ggaaccgttt caaaacctgt
     9661 tggtgtataa aaatgaccta gtttgatatt gacaccattt ccaacaggta caagggcttc
     9721 gagataagct tggggaaggg caattccata agtcttggtt gatgcacaac atagatctag
     9781 atcccagtta cttcttttaa gcgcttctcc tgaattaaca tcaaaggttg gcacaccaaa
     9841 cgcctgagta aaaatggcat cggtaccaaa cataaaatca aaccgcccgc cgaaatgcca
     9901 tgcttttcct tccgaaggca cggaacgctg taaaaataga ttaaattgat taagctgaaa
     9961 tcggttggcc tgatcagcaa aaataacggg gccgttataa ccaactgact ggcttggatt
    10021 aaaagttgca ccgccatgta tccatccgcc tacttcaatt ctgttacttc tcagaaaaga
    10081 ttttagatta tctgcccgtg atataagtgg atagccagca ataaaaaaaa ttgataataa
    10141 tgatataacc ttaagccaac gttcccactt catatcattc aagcaagaaa aataaatact
    10201 cgagaaactt gaatctctcg tattagcact attagaaaaa agattttata catgcgaaag
    10261 cgcccttcct cggtaaagga agggcgcttt gtttttagtt aattaagttt cgtgtactta
    10321 atgaaatgtg cgcgtcacat tgctgttatc aacatttgca cttttgatac tatcattttg
    10381 ctcactcgcc atttttctgt ggagttcagc agctttaaga ttttcagtaa tgacttcttc
    10441 gtaatattca atattagctg tttcgtgcga ttgaaaatcc tgaccttgcc ttccataata
    10501 ataactatgt acttcgtagt cttcgagatt ttgtttatgt tcagcaagct tcaattcggc
    10561 ttctttcaca atattctcgt gataagtaac caatgtttca tgactcgcat tagtaagatc
    10621 tgtttcagca aaagcagaag cacttaatac atatccaaca actaaaacta agagattaat
    10681 tactttcatt gctgcatccc ccgtgaggtt aataaaattt ttccattggt gttatcgtaa
    10741 ttcaatgaag gctagttagt atattgggga aaatttgatt tttatctaag ggtttccctt
    10801 agatagcaga aatgaattgt agccaatagg caacctggaa atattaacgc aaaggctcct
    10861 atcattgatt ggtgattaga tttttgtgcc aatgacttta ttgtattagg atatggccta
    10921 atagattttt tattagcgcg atgggaactc atttccgcaa actagacaat cagataccaa
    10981 catagcacta tcttcttacc taacgaggag cattctatgg atcaaggtaa cctatctttt
    11041 tttaaactac tgtttattgc cagtattatt ttgttgctag ctgcttgcac tacaatgcaa
    11101 tctatatctt ctacaagcga accgcaagaa ccaaaaattg gttttccagc aaaaatttca
    11161 cttgtctcat tggctaaaga atttggcact attgatgaaa cgtttaaagt tgaaaacaat
    11221 gagtgcgtgg tgattgataa agaaaagcct gagaattcag caagcttatt taataagctt
    11281 actgacaaac tgatcaaaga aggagctgta aatcgcgcgg atttagatga gcaacaaatc
    11341 aaagaagcgt ttaatcttgt taaatcagga gaatttaaga atgatttaaa tgatacgcga
    11401 gattctttga taaaaaatat tagggttttt cagcaaagct taagaacaaa gataaaagag
    11461 actgtcacta atccccaatc cgcttttgat ttgatccgtc attccccaga atatctgcag
    11521 atcaatccca gtaataatgc gcaatgcgag caagggatta aagcactact cacgcatcaa
    11581 aattatgaca acctaggtac ttgcgcgcca ggtgcagttg gaaagattat tgataaatta
    11641 aaaacggata aaacaacttc agcaataatc gaaactgcac atatctttac taacaaaaat
    11701 aatcgatcca gcagacttgc tatcatcgcc tatgcaagaa tgcatggcat caaactgacc
    11761 gaagatgatt tggataaact agatcagttt ctagacacga cgaatgaatc tgatctgtct
    11821 ccgttgatca aagtgggtat cgataagctt gccgaaaaat acggaggcaa agatattcaa
    11881 gcgttcatcc gcaaaaaaga tcaggaatgt agtctcaatt gatctgtaag taggcaaggc
    11941 atcaataagc aatgccatga ctcattttct gccatgtttt gaggatctgc tcgcactcat
    12001 cacgtaaaaa ttcactcaca atctcaactt gactatggcc ttcgcgtttc atgtactcat
    12061 tgctcaaatc gagctcattg aaacctaatg tcttgacatc tgcaatacgc gttccaaaca
    12121 cgatcgtatg aattctagcc caatgaatag cactgaaaca catcgggcaa ggctcagttg
    12181 tggaatagat tacacaatca ttaagatgat gttgattaag cgctcgcgca gcttgacgaa
    12241 tggcattgat ttctgcatgg caagtgggat cgtgctcacg taacacagta ctatgcgcca
    12301 cggcaatcac ttcgttaccc tttacgatac acgctccaaa tggtcctcct tccgctaatc
    12361 ccatgaaacg ctgagcatca tcgatagcaa accgcatcca tgtcgcttga ggtacaacga
    12421 ttcgatccat ttcatcacac cattacaaaa ttattttagg aaaaacccta acgtaacgat
    12481 cacataaaat caagatttgc gctgatcagt cttgaattgc actacctcta ccacgcttct
    12541 cgtcaatacc gatcaaggat agtaacccaa ccataaagaa acatgtcgtt accaacagtg
    12601 ccgtgcgatg gtctccctgc gtaacatagg tgatcaaccc atagcacaat gggccaatca
    12661 ctgctgccag tttagtcgct aatccccata aaccaaaata ttcccctgct cttgcaggag
    12721 gagaaaataa tccaataatg gcacgcccag cactttggct tgatcctaat gcgataccca
    12781 ctaaattggc aactacccag aacatcatgc ggtcatatgt aaaataggcc agcaacaatg
    12841 cgcagatcca gataactagg gtcatcgata acgttcgcaa cgaaccaagt cgatcctgaa
    12901 tttgcccaaa gacaaatgcg cccagcgcag cactgatatt aacgattaaa atgagttgga
    12961 tattatcttc tgtcgtaaaa cccatcgctt cttgcgcata aacagcggcc agcacgatga
    13021 ccgtgttaat tccacaggta taaattgtca aggcaatgag aaattgaaat aaatcccgat
    13081 gaaaacgcgc gtgctgccat gtcgtgtgta ggcgttggta agcaatcgag caataattga
    13141 tagcagttcg ctcacaaggt tggacacgtt cgcgcaacca gatgaaagta ggtaagctcc
    13201 ccacgacgaa catcgctgcc acaatcagca atgtcagtgg cacagcatcg gccatttctg
    13261 ccccctgcgt cgcagtgttt aaggtaaccc atagacacaa caccaaagtc agtaagccac
    13321 cgagataacc aattgtccat ccaaacgctg aaattcgacc cattttatct ggcgttgcaa
    13381 tttccggaag aaaagcagca ataagattct cgcccgtaaa aaaacaaata cttgccaata
    13441 tcactaaagc cattgcgtaa cttacatcac caggacctgc ggttgccagc aacgtggtaa
    13501 agcctacaca gccacttgcg gaaatcatta gaaagcgttt tttccaagca cgatcgtctg
    13561 cgatcgcccc gataatgggg gcggtcaata aaacaacacc attggcaatt gctgtagtaa
    13621 tggtccacca cagcgtcgca gttccagaag aataagcttg tgcaatcaca ccaacgaaat
    13681 aagcattgaa tatcgcggtt aatacaacgg tggtataacc agaattggca aaatcaaata
    13741 gtgcccaagc aaaaatttcg tgtcggtgta ccggtgcttg gattaatttg gacatcgttc
    13801 gatttatctt ctaggccatt gaatttataa tttaaagtaa tacgataagc caaatattga
    13861 actgagcatc acttaccgag tgttactgca aataaaactt acacacttta gctgcttaaa
    13921 taatacgatt gttagtgacg cggtgatgcg agtgacgcaa tgctgagaag aaccccagcc
    13981 taaccaagat taaggtgatt gtttcgcgag tatctcaatg gcagtgagta actgatcacg
    14041 aaattgttca caaggaatca atgctaagaa cggtgcgcgc gctgtttcac aatcaggatc
    14101 catcggacca taatccacgc gtagcttacc ccttttttca gtattcagat aatacatatc
    14161 tcggtgcttg gtatctttgg gtaaggcaat atagaacaca tcactttctt catcgacttc
    14221 gggtactgtt agtgtttcat attcttcgat ttgtaaatct tgatagagat ttcgtttttt
    14281 gatcaactca accactaacc gataattctc attcatccca aaataaatag gatcttgtcg
    14341 caaatcttcc atcgtaaccg gaaaattggg attggattct ttgatgttat cgaaatgagt
    14401 tctgaaaatc gcttcctgtg aaggaatcaa tgcgatcaat tttcgttgta tcggtggagg
    14461 gttaagggat atcgtacctt caatacagcg ttgatacatc tcagcacgag cagcgatata
    14521 ttgtcttctg ccttcatatt ctttgccctc aaggaaataa atcgtactgc aaccagttaa
    14581 tccgatcagt atcagaataa tgacccacac cctttttttc ataagaatct tcaccttatt
    14641 tcagtactgc ttctatcttc catatatccc gcaacatcgc aataccatga gctccctgtt
    14701 caatcgcgaa aggtaaatct gcttgtatca tgccacccaa tgcataaacc ggaaggggat
    14761 aatcccgaat catacttgaa aattttctcc agcctaatgt aatcgcatcg ggatgactct
    14821 ttgtcggtaa tactggaccc aacaccacaa aatctaattc aagttgttca gcgtgataaa
    14881 gctcttcgat gttgtgacaa gaggccccgc accaagccag atcgggtcgg acagacatct
    14941 ccatcaattg gctagaagtt aaatgcacac cactggctcc aaccgcgttc gctagcgcta
    15001 tatcctcatt tatcaaaatc ttcatttgat acgggtgagt acgcttaaaa atcgcatcga
    15061 taaattgttt tagctgtgtc ttattcatcg atttctcgcg aacctgcagt agtcttaagc
    15121 cttgttgtgc tgcgagttca attttttgca gcatggttga ttcgcccaat tcagcggctt
    15181 gagtaattcc ataaacagag ggtaaggata ggctacgaat aatcggctga ttagcaggaa
    15241 gcagcggtgc aacaataacc ttgtcaggcg tttgccagga caattgctga ttctcacgac
    15301 caataagctc accatgccaa gtaaacacgc ggtagaaatg cagtcttact gttgcatgct
    15361 ggtaagcata aatttttgtt atccaagggc ttgcctgctc aatttgaatt cctagctctt
    15421 cgtacagttc acgtgcgagc gcttgtaaaa cagattcatc tttctcaatt tttccaccag
    15481 gaaactccca ataacctgaa tagggttttc ccgctggtcg gctggttagc aaaaaatgcc
    15541 catcgggtcg aactaacaca gcagcagcaa cttcaatcat ttactcaaaa ttatgagggt
    15601 tgacgagtct tgcggcgtag tgattgtgct aattttcgtt tgccagacca atcacgcgca
    15661 aactgccagg caatacgccc gcttcttccg cctcttgcta atgcccaacg caatgcctct
    15721 aacctaacgg catcattcat atgctgaata ccgtaatgcg ctagccaata gcgaacgatg
    15781 tcgagatatt gcgtctgatc aaaaggataa aaagaaagcc ataaaccaaa ccgttctgat
    15841 agcgaaattt tttcttctac ggactcactc ggatgaatat caccatcgat gtgacgtgta
    15901 tccaaattat ctttcatcga ttctggcatc aaatggcgtc gatttgaggt cgcgtaaacc
    15961 aagatatttt ccgatgcacg agcaatcgaa ccatctaaaa tcgctttgag tgctttatag
    16021 cctggctcat gcatttcaaa cgataagtca tcacaatata gaataaaacg ctctggtctt
    16081 tgataaattt gctcagtaat atcgggcaag tcgattagat cttgcttttc tacctcgatt
    16141 aagcgtaacc ctttatccgc atattgatgg agtaatgctt ttactaagga agattttcct
    16201 gtaccgcgtg cacccgtcag taacacattg ttagcaggat aacccaagac gaactggtag
    16261 gtattctgaa cgattaattg cttttgttca tcgataccct gcaatgcatc gagctgaatt
    16321 tgatgagggt gtgttaccgg ttgcagataa ccattatgac cttgtttgcg ccagcgaaaa
    16381 gccgtcgctg cgtcaaaatt tgtcatatcc tgctgattag gcagcaatgc ttcaacgcgt
    16441 atcagcaaat cattgagccg atcaagaacc aaatcgaatt gtgacatcat caatcaactg
    16501 cgatagtcag cattaatctt gacgtagtcg taagaaaaat cgcaggtcca taccgttgct
    16561 gtcatggtgc ctcgatttaa aaccacccgt accgtgattt cagcttgttt catcacccgt
    16621 tgccctgcct cttcttgata actttgggct ctaccgcctt gttctgccac caacacatca
    16681 tccagataga gctgcactaa atctacatct aaatcgatga ttcctgagtt accaattgca
    16741 gccaagatac gacccagatt agggtcagaa gcgaaaaaag ccgtcttcac taacggtgaa
    16801 tgtgcgatgg catatgcgac ccgtgaacac tcttctcggt tctttccttt ttccacacaa
    16861 accgtaatga atttggttgc gccctcgcca tcacgcacga tcatttgtgc taattctctt
    16921 gccacggccg ttatcgctgt ttgaagcttc agataatctt ggctttctag ttgagtaatc
    16981 tctgagtgcc cagctcgtcc tgtcgccatt agtatcagcg catcattggt agaagtatca
    17041 ccatcgactg tgatacagtt gaatgagtga tccgctacat gacgaaccat ctgttgcagg
    17101 atagccttgg caacgcaagc atcagtgcca atatagccaa gcatggttgc catattgggg
    17161 tggatcatac ccgagccttt tgcaattccg gttatagtca ctgtttttcc ttgaatttca
    17221 atttggcgcg acacgccttt gggaacgata tcggttgtca tgatcgcttg cgcagcctta
    17281 aaccaatgat ctgggttaag gtctgctaat gcctgcggca aatgagtcac aatcttctcg
    17341 accggaagcg gttccataat tacaccggta gagaagggta gaatctgcgc tacatgacaa
    17401 tttataagct tggctagatg ctgacatgtc gcacgggcat ctgcgatgcc ctgctctcca
    17461 gtgcctgcat tagcattccc tgtattgata accaaagcac gaattgaaga ggcttgggtt
    17521 aaattatcct tggctacaat cactggtgca gcacaaaacc gattttgcgt aaaaacacct
    17581 gctacattga cgttttcatc taacacgatg gtcaataagt ctttacgatt tgggcgctta
    17641 atccccgctt ccgcaattcc aagggatatt ccagcaatag ggagtaattg ctcagaaagt
    17701 agtggcgaaa tattaactgg catacgttaa aatataaaaa aatgagttat cgtatctcaa
    17761 cacgattgag atattttgaa aatctaaaaa tagatgctca ataagaaaat tatgaaacta
    17821 actaaaacac aaccattgtt agcaaaaaga gaaaacccat tccgtgctga tcgcgaatta
    17881 gacgtacgtg gactagtctg cccactgccc atattacgca ctaaaaaagc cttgtcagaa
    17941 atgtcaacag gacaagtcat caagatcgtc gctacagatc ctggttctgt aattgatttt
    18001 caggtttttg ccgaacaaac tgcgaatgat ttattatctt tcactgaatc agtcgatgaa
    18061 ttcatttttt acctaaaaaa gcgctaatga ttggctaatg agcgcttaag gccatttaaa
    18121 gcccagcaac catcatccga tcaattaata ttgacccaca ttgtttggat ccgcggataa
    18181 tcacatcgct acccacgcca atgatacccg atagcatgac ttttagattg cccgcaatgg
    18241 taatttcttc aacggggtaa cctatttccc cattctctac ccaaaatccc attgcacctc
    18301 ttgaatagtc cccagtcaca ccatttacac catgacctaa taattcagtc acgagtaatc
    18361 ctctattcat ttttttcaga agcgcctcaa agccaagcgg ttcagcacac gacaatataa
    18421 gattgtgatt accccctgca tttcccgttg aatgtaagcc cagctttctt gctgaatagc
    18481 tattgaggaa ataaccttgc accacaccat tctcaataat gctacgatct ttcgtcgcga
    18541 ttccttcttc atcaaaagta cagcttgcta gacctttggg aatatggggt cgctcgtgaa
    18601 gctgaatatc agacggaaga atttgtttac caatgctatc caataagaac gatgatttac
    18661 ggtataaact cccaccactg atagcgctta ccatgtgccc tattaaactc gaggcaaggg
    18721 gcgcttcaaa taacacgggt acttcgcacg ttgaaagctt cttggcccct aaacgtgcaa
    18781 ccgtgcgcat tcccgctttt ttacccacag cttcaatctg ctccagatca tgcgcatctc
    18841 gtgcaacact aaaccaataa tcgcgttgca tcatatcggc ctcactcgca atcacagcac
    18901 agctaatact atgtcgtgaa acaggatatc ctcccataaa acccaagcta ttggcgtata
    18961 caaattgaga agcgccaact gaaactgagg ctccttcaga attcgtaata cgtttgtcga
    19021 caacgagcgc ctcggcttca cagcgctgag ccagttcgat agccgcctca acgggtaaat
    19081 tccatgggtg atacaaatcg agatcagaaa atgtggttgc caataaatta gcttctgcaa
    19141 ggccagcaca attatcttct gcagtatgtc gcgctatgga caatgctgca gcgactgtat
    19201 cattgatagc ttggggtgaa aaatcagaag tactggcatt gccccgtttc tgacctatat
    19261 aaacggttac cgataatcct ttatctcgat tatgctcgat cgtctcaact gcattctggc
    19321 ggacagtgac atttaaacca aaaccatctg acacgtttgc ctcgcaggca gttgctccgc
    19381 ctcgcttagc gtgattcaaa atatcatttg caacttgctg caattccgca aaagaatagg
    19441 aaaaattttg tccgctcgaa gtatctggat tcataccgcc tttacttatt taaactatga
    19501 aatacattag caccgcgcta aaagcaacgg tgcgtgaaat tcaaaaaaaa ttttacaaaa
    19561 ccgctatgat agcagcttct tcacacagct ataaatcgat ctgaaatgat acacgatagt
    19621 catgatgaca atgaatccga agaaattctc agtaagacac gccgtaagaa agcaatgcat
    19681 gcactccaag gaattggtga gcaacttgtc gaattagatt cccaattcat tgctgaactg
    19741 gatttaccag agatattggt cgatgcgatc cgagaagcga agcaactgac caaacatgaa
    19801 gccaaacgac gtcagatgca atatattggt aaactgatgc gtaatatcga cgctgcaccg
    19861 attcaagaaa aaatcgatta ctggaaaaat cgtgacgtta atcataccgc ttggttacat
    19921 cagttggaaa actggcgcac gcggctcttg gctgatgaga attcaattaa tgaattcaaa
    19981 cagcactatc ctgaaactga tatacaacac ttacgagcct tgattcgtaa tgcccataaa
    20041 gaaacacttt cgcagaaacc acctaaaagc ttccgtgcaa tttttcaaga attgcgcacg
    20101 attattccag agaaaaaata accgaataag ttttaagtta atgtgagaat ccagcaaggt
    20161 tgagttcacg atcttttttg attgatgata attcaagatt ccaaatggct gtagctaaag
    20221 cttgggaaga ctggttggct gttttggaag aagattcgtt cgatagtaag ctagatggtc
    20281 aggaagattt aattgataat ttgactttgt cttgtcctca aatttttcaa aaagattgtt
    20341 taactctata ttagatggcg tctagaattt agatgaaaaa taagaatttt taattcttgc
    20401 tgagaagtaa atccaattat ttgattttat tgatctagaa aagaaaaaaa acagatttag
    20461 ataatgctta gtatagtaat ttaaaagcat tattccttca gtttcttttt tttgttatac
    20521 aatcacgcga tacaagcgcg tagttaaaaa actgcgtgac aaatttttag agatattatt
    20581 gaaagggagt gtatgaaaaa ggtgttacaa aataattgga tagcgaaggg attcgtggtt
    20641 attttcatgg tattggcttc gttttcattg aaagcacagt tgatgttggc ccatgaagga
    20701 catcatgcag ggcatggcgg ctgtgaggta aagacaggtt ctttcgttgt aacagtaagt
    20761 atttatgagg tgcctgaagg taatattcca ccgatgcatt catattgcaa tcatgtacct
    20821 aaacccggaa aagtgaatgt aacgatcgag ttgccatcag atgctcgtga gattccacta
    20881 gcagttcgtc tggtgaaaga tgactcgcat gcaggacacg gaagtggatc tcctcaaagt
    20941 aatgcaactg agaaagcagc gagtgctgta aacgaaggag atgatcatgc gcatcatcac
    21001 gatgaaacgc atggcgcagg agagtttggt ctgcattata tgccgccaat ggaacatcac
    21061 tctggaatta ttgtggtcgc agcggagtta accgagctag ggcaatatgc gattttgctt
    21121 gaacaaatcg atgataaaga tgatgtgaga atagtttcca aagtgccggt tcatgttgga
    21181 ggtgatggag gccatgctgg ccatggatca ggtattggtt taatggaaat tgtaattggg
    21241 ctcatgctgg ttgtgggtgg aatcggtgcc tttatgttta gaaagaaaaa gccacaagca
    21301 agctaatcta aatttaaagg tttgagagtg aatcgaacag gaagtaacgg gaagagttaa
    21361 gaaaaggagg agagaataga tgaag
//
LOCUS       DAOQKQ010000021        21283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_1074_length_21283_cov_5.903901, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000021 DAOQKQ010000000
VERSION     DAOQKQ010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 21283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21283
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1074_length_21283_cov_5.903901"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1386)
                     /gene="rpoN"
                     /locus_tag="PKZ49_02455"
     CDS             complement(<1..1386)
                     /gene="rpoN"
                     /locus_tag="PKZ49_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HND35451.1"
                     /translation="MKPTLQLKLTPNLTLTPQLQQSIRLLQLSTHELNQEIERMAQEN
                     PLLELENTIHEQPYRDDAPSESQSSPTLSNEEGVQSEDSYLNNNELSPSSASEIELEW
                     YGDQDSPYTKRSDDDDEWDLFQQTAESISLREHLATQISLSQIDDRERRIVGLLIDSL
                     DDAGYLSQDLMELSELIPSELGIQLKDLETALKHLQRLDPPGIGARNLRECLTLQLQS
                     LPETTPYRDKALQLVMTDLECLAAKDFRQIKKMLQCDDHCLRAIQQLIIHLNPRPGEA
                     YKNDFARYIVPDVIAAKSEGAWIAKLNPSSLPRLSINHLYANILKHNKNESTQSLIGQ
                     LNEAKWLIKNIQQRSDTILRVSNEIVTRQQDFFENGAVSMRPLIMREIAESLELHEST
                     VSRVTTQKFIHTPHGIFELKYFFSSHVATDTGDTCSAVAIRSLIKQLIQVENQKKPLS
                     DLQISKILGERG"
     gene            complement(1421..2143)
                     /gene="lptB"
                     /locus_tag="PKZ49_02460"
     CDS             complement(1421..2143)
                     /gene="lptB"
                     /locus_tag="PKZ49_02460"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HND35452.1"
                     /translation="MSILSAKNLKKSYKSRTVVKDVSFSVASNEVVGLLGPNGAGKTT
                     CFYMIVGLVPLCGGEIYLDDRNLSKLPIHERARLGLSYLPQEASIFRRLSVEKNILAV
                     LELQNLSADEIQQRLDNLLHDLHISHLRENMGISLSGGERRRVEIARALASQPNFVLL
                     DEPFAGVDPIAIIDIQKIIRFLKDQGIGVLITDHNVRETLGICDRAYIISDGTVLASG
                     KPDEIINNEQVREVYLGEHFRL"
     gene            complement(2177..2707)
                     /gene="lptA"
                     /locus_tag="PKZ49_02465"
     CDS             complement(2177..2707)
                     /gene="lptA"
                     /locus_tag="PKZ49_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HND35453.1"
                     /translation="MVVGVLPSSLALAERADRNKPIQLESDSATVEDYKRKDTFRIST
                     FVGNVILTQGTIVIKADKIIMKEDLKGYRYATAHGDLVSFRQKRDGANEFIEAWSQRV
                     EYDDKTDKIELFGKARLKRGPDEVKGDYISYDVARDFFQVKGNNTKNEVENPDRRVRV
                     VIQPKATPKIEDDSEN"
     gene            complement(2799..3365)
                     /gene="lptC"
                     /locus_tag="PKZ49_02470"
     CDS             complement(2799..3365)
                     /gene="lptC"
                     /locus_tag="PKZ49_02470"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HND35454.1"
                     /translation="MIRRLLPGPVIILAILVLFTFWLDQSIQQPKKRYDTDQTKNPDY
                     VIENLSGIQMVYAQAAERHFTAKILKHYPVTNVSALEHVHFTHNQTNEPTLKINADSA
                     ELSKGNEDIYLFGNVNVVRGDEANKDIVTMVTDSLHLVPDQEIVKTDRPVTITKMNTI
                     VNSVGMQLNNKTGIIELHSKVKARDVKL"
     gene            complement(3661..5373)
                     /locus_tag="PKZ49_02475"
     CDS             complement(3661..5373)
                     /locus_tag="PKZ49_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HND35455.1"
                     /translation="MIRSTLSLLFLAIGTYSMEIQANPVNISKDLAITTEETLTDPIP
                     EPILKGHLRLRLKLIAQGLTAPNWAIPAPGDTDHLYVGDQNGKLWKINLLDGNKESFL
                     DLSQLLVPLGIFGENSFDERGFLGFAFHPQYTQNSLLYTYTSEPAGEKSDFSTISSGS
                     NPNHRSVIREWRINSANLAKDMPNRVLLSIDQPQFNHNAGALNFGPDGMLYIALGDGG
                     GADDRDGQPFIGEPIIGHGTHGNSQDLTNPLGSLLRIDPSGTNSINGQYGIPNDNPFS
                     NSNNALGEIYAYGFRNPFRFSFDSVSGALLLADVGQNTIEEVNIVKSGGNYGWGLKEG
                     RFRFEPNGNGPGFITNAPIIGDFIDPILEYDHDEGTAIIGGFVYQGKMIPELKGHYVF
                     GDVAKTSNGDGRLFYSDGSKIQELDLTQQSQPGFWILGFGQDNDGELYVLGNSTGLPF
                     GSTGVVYKIVPNSHFDGTEVAIPAVKVITSIDKAETYHARLRLIPDSQPLRFELIHAE
                     KLPDRIIDTDNAIFNSAMGTLNLPFINIVSQGSEVTTYQVQLRQVSNQPTLTFELTQS
                     VLIR"
     gene            5536..>5855
                     /locus_tag="PKZ49_02480"
     CDS             5536..>5855
                     /locus_tag="PKZ49_02480"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HND35456.1"
                     /translation="MKATVTELLQRMPGKVSEEWPMGEPFAVALAHGSMSVELYAPKD
                     VDIQTPHDQDELYFIHSGKGEIVIAGNRYAFEPGTVFFVPAHIEHRFENFSADFATWV
                     VFWGP"
     assembly_gap    5856..5955
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6083..6790
                     /locus_tag="PKZ49_02485"
     CDS             6083..6790
                     /locus_tag="PKZ49_02485"
                     /inference="COORDINATES: protein motif:HMM:NF019311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HND35457.1"
                     /translation="MFQRKIMLVTILFTFLLVDVTAAPVPVGPALMGSGDGLKMRWVN
                     TDFSPHSITDAAQALNLRPGDVGYVGEVSGVTKVIDFEDGNYGDLFRDTDQAVPLGAD
                     DNFAVLYKGFLNVTVSGNYTFRSFTDDGFRLTIGGEVVSLFNSDRSPGETTTSVFLNE
                     GLYHFEFIGWEQGGQFVNELAWIKPGEVEFTQPGDRTNLVFFTTQGDASVPEPMMLSL
                     FLIGLIVMGYARQSRQS"
     gene            complement(6841..10230)
                     /locus_tag="PKZ49_02490"
     CDS             complement(6841..10230)
                     /locus_tag="PKZ49_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HND35458.1"
                     /translation="MKTETRLIVAICLTMIYSLFAMSASASDETFHHQMQITLIPSAS
                     EIQVTSTIQVPKHLLKSDSETKLDFSLHADLSITEMNGAKLSPQQKINTLLARPVPVK
                     RYTVTLPAQQNSFQLSFQGKIHHTVQGPGEEYARSFSYTPGLISEEGVFLANSTAWYP
                     QFENAMVSFQLEVKVPQDWDAVSQGTLLSEQKSSTTQQVVWEEKHPQDDIYLIAGRFE
                     RYTQPATTTITSYVYLRGSDKALAQKYLDTTAQYIAMYNKLLGPYPYTKFALVENFWE
                     TGYGMPSFTLLGPKVVRFPFILHTSYPHEILHNYWGNGVFVDYTQGNWSEGLTAYLAD
                     HLVAEQGRKGEEYRRDVLQKYTDFVNKEKDFPIKQFTSRHSSSSEAVGYGKTMMFFHM
                     LRQTLGDDDFVRLLRAFYQQYKFKQATFQDLLASINHYTRSDFTPFFAQWINQSGAPN
                     LHLQEAQAEPAANGFRLKVTLKQTQPGKPYDLTLPIAIHLEGQPQAYQHSIKLNQTVQ
                     TISIDLPQRPVRIDIDPQFDVFRRLDSREIPSALSQGFGAENALVILPSKADPALLQA
                     YQQLAANWQKTQSIRLEIIQDHQLDKLPVDRTIWVLGWENRFSQPVLSTLESQKVSYQ
                     ANTLQIAQQHYHQAEHAIVLTARQPENADKTLLWVAAHHPSAIAELARKLPHYRKYSF
                     LVFKGDELTNIDKGQWPITQSPLTQKIHQQDDPSFASDHVGSLSPRRTLAELPPLFSE
                     TRMLADITHLASATFKGRELGTPELDLAADYIAQQFHQAGLLPGGDNQRFFQVWQHDV
                     GPPKGIVSLRNVVGILPGSNPQLDKESLVIGAHYDHLGMGWPDVRHDNQGKIHYGADD
                     NASGIAIMLELARQIAPKWQPQRTIIFIAFTGEEANLLGSTYYANHDNNYPTKKIIAM
                     LNLDTVGRLGNNPVTIFGTGTAREFVHIFRGIGFVTGIPINSVLDDFGSSDQAAFIKA
                     GIPAIQLFASAHEDYHAPGDTVDKIDASGLVKVANVLKEATEYLANRHDPLTVTIQTT
                     ESRAQIKPMTEKRKVSLGTVPDFSYQGKGVRVENVMPGSPAELIPLQHGDILIALANQ
                     PISDLATYSAILKTLEPGQQTTLQFMRDGKTITVDIMLTER"
     gene            10379..11479
                     /locus_tag="PKZ49_02495"
     CDS             10379..11479
                     /locus_tag="PKZ49_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter Tol"
                     /protein_id="HND35459.1"
                     /translation="MEKHVQSFKRSSWSIGFLSLALLCLAMSVTASDFIVEDHQLSDK
                     EVRVGEAYYRADMKWMVYQAEVSESNPFYQIYLKDLVSGKVQQVSPGDGKTTCAWVHP
                     SQEKVLFSSTHEDSDAKAKMLAEIERRKSGEKQSYAWDYDEYYDIYETDFTGGRIKNL
                     TNTLGYDAEASWSPDGKLIAFASNRRAYTEKLSDEEAVLLKKDPSAFIDIYIMNADGS
                     NVKRLTHTNVYDGGPFFSPDGKRIVWRRFSTNGREAEIFTMNIDGSDQKQITHLNMMS
                     WAPFYHPSGQYIIFSTNLHGHRNFELYIVDVDGKKQPVRVTDKDGFDGLPVFTPDGKA
                     ITWTSDRTPEKKGHLFHGKWDHTKALESLSLQ"
     gene            11609..11857
                     /locus_tag="PKZ49_02500"
     CDS             11609..11857
                     /locus_tag="PKZ49_02500"
                     /inference="COORDINATES: protein motif:HMM:NF018961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HND35460.1"
                     /translation="MSIAKVFEIISSSKVSFDDAIKKGVARAADTMTDITSAWVKDQS
                     VEISGGKIVEYRVVLKITFVLKPAPKAAVSKTKAKATK"
     gene            11894..13018
                     /gene="earP"
                     /locus_tag="PKZ49_02505"
     CDS             11894..13018
                     /gene="earP"
                     /locus_tag="PKZ49_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HND35461.1"
                     /translation="MGWDLFCRVIDNYGDIGFCWRLSRQLTTEYGLAVRLWVDDLASL
                     QRICPSVDPHQTFQQRCGVQINHWTDPFPDIIPADVVIEAYGCELPEKYIQAMLPSHS
                     RSRQPVWINLEYLSAESWVEEYHGLCSPHPRLPLTKYFFFPGFTANTGGLLREKDLLV
                     QRDSVQHNAANWWLQNDLPIPDETEIVISLFCYDTAPISNLLEAWAHSQTPIRCVIPE
                     GQALTLLRNGLNRSALKAGDVIQQDKLTVQIIPFLPQAQYDQLLWLCDCNFVRGEDSF
                     VRAQWAARPFVWQIYPQADNVHYVKLEAFLNRYSQSLTKESAQALRAFHHGWNGSERL
                     DWNHFWQHHAIFHQHALTWSSQLAVIPDLTFNLVEFCKRQ"
     gene            13157..13762
                     /locus_tag="PKZ49_02510"
     CDS             13157..13762
                     /locus_tag="PKZ49_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HND35462.1"
                     /translation="MRISFDFGELKLRMTGRTQATALRAIYQLKVTLNYIRPPIWRRF
                     LIASTDSLANLHLVVQIVMGWSDSHLHEFAKGGERYGLPDEDFPSAIHDESKFRISQI
                     LKQEKDKIRYTYDFGDNWDHEIVLEKILPFEMGTKLPTCLKGSRACPPEDVGGPPGYE
                     MFLEALADPSHPEHGGILEWFGEPFDAEHFDLTEVNHLLHN"
     gene            13951..14928
                     /locus_tag="PKZ49_02515"
     CDS             13951..14928
                     /locus_tag="PKZ49_02515"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HND35463.1"
                     /translation="MKTTFLDFEKGIEEFEEKIEQLRFAQDNSALDISAEIAKLQAKS
                     QALTKSVYAKLTPWQISQVARHPQRPYALDYIQHLFTDFEELHGDRNFADDHAIVGGL
                     ARFNGQSIMVIGHQKGRDTKEKIYRNFGMPKPEGYRKALRLMSLAEKFSIPLITFIDT
                     PGAYPGIDAEERGQSEAIGKNLYVMAGLEVPIVSVIIGEGGSGGALAVAVGDMVLMMQ
                     YATYSVISPEGCASILWKSAEKASEAAEIMGITADRLKEMSLIDGIINEPIGGAHRDY
                     PATMLSIKNVLQESLRKLQDISIESLLERRFNRLMGHGKYKEVKPERHT"
     gene            14888..16249
                     /gene="tilS"
                     /locus_tag="PKZ49_02520"
     CDS             14888..16249
                     /gene="tilS"
                     /locus_tag="PKZ49_02520"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HND35464.1"
                     /translation="MGSIRKSSLNGIRDTVEVVLRQYVSEQNHLALALSGGVDSVVLL
                     QILAQISKQFTFNLSAIHVEHGISPNAKQWSDFCQSYCDALGVPLTVFHLSIKKQSQV
                     SLEAAARRGRYAIFKAISADFIVLAHHLDDQAETLQLQLLRGAGLRGLAGMPIMRSLG
                     SAANNVKLLRPLLTVSRDIIQDYALQNKLMWVTDESNDDTTYHRNFLRHQIFPVLEQR
                     YPAYRETLARTSRHIGEAIQLLDGLAENDSQSVMVMGDIHIERLRKLDFLRAKNLLRY
                     IFSRHDIIQPSTQKLEEILRQLWAVKSDNHLHLVFGEFEIRLYKNLVKLLPKAKPPDF
                     DQDQLFYWHGELNWLIEPLGGVIKWSNSTGTGINAKKLMENPVAVRLRKGGERFQPNC
                     RRPRRSLKKIFQEAAIPEWERNDLPLLLSGDQLVWVAGIGIDCGFQVMPGEQGLMPSW
                     HPS"
     gene            16275..17321
                     /gene="rfbB"
                     /locus_tag="PKZ49_02525"
     CDS             16275..17321
                     /gene="rfbB"
                     /locus_tag="PKZ49_02525"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HND35465.1"
                     /translation="MILVTGGAGFIGANFILDWLSRIDEPVVNLDKLTYAGNLQNLIS
                     LQSDARHIFVQGDICDGQKLNQLFDNYQPRAVIHFAAESHVDRSIHGPDDFIQTNIVG
                     TFRLLEAARHYWNKLTGKAHYDFRFLHVSTDEVYGSLAPDVPAFTEKHPYSPNSPYSA
                     SKASSDHLVRAYHHTYGFPTLTTNCSNNYGPFQFPEKLIPLVIVNALAGKSLPVYGDG
                     LQIRDWLYVKDHCSAISRVLEAGVPGETYNIGGWNEKPNIEIIRLICDLLNEFCPGDY
                     HRLITYVTDRPGHDRRYAIDAQKIESELAWRPLETFETGIRKTVQWYLAHKDWVANVQ
                     SGEYRKWITTHYQQ"
     gene            17327..18223
                     /gene="rfbD"
                     /locus_tag="PKZ49_02530"
     CDS             17327..18223
                     /gene="rfbD"
                     /locus_tag="PKZ49_02530"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HND35466.1"
                     /translation="MKILLFGKNGQVGWELQRSLAPLGRVIALDRFSQEYCGDITDTA
                     GITQIIRAIAPDVIVNAVAYTAVDKAESEPVLARQINTEATAVLAHEAKRAGAWLVHY
                     STDYVFNGSGNKPWLETDNPAPLNVYGQTKLEGEEAIRETGCLYLIFRTSWVYAVRGN
                     NFIKSILRLANERDQLTVINDQIGAPTGADYLADTTAHAIMMAINRPELSGLYHLVAE
                     GEISWHGYASFIINFAQAQGIDLKLKTNAIVPVPASNFKTPAKRPCNSRLSTDKFQKT
                     FRLNVPQWQIGVSRALTEILER"
     gene            complement(18246..19196)
                     /locus_tag="PKZ49_02535"
     CDS             complement(18246..19196)
                     /locus_tag="PKZ49_02535"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HND35467.1"
                     /translation="MTELPNQKEGWEKELLEKVVLSTLREQRRTRLWSIFFRSLAFAY
                     LFILLFWGMGLFSDDSTSSSDRHTALIDVRGEIMADSLSSADNVNAGLQKAFKDKNTA
                     GVIIRINSPGGSPVQAGNINDEIRRLRTKHPQIPVYAVVEDICASGGYYIAVAADKIF
                     VDKASIVGSIGVVLDSFGFTGTLEKLGVERRLLTAGENKSFLDPFSPSDPKQREHARK
                     MLGEVHEQFIRIVQHGRGDRLKDSPEIFSGMVWTGQKSIDLGLTDELGNTEYVAREII
                     KAERIIDYTTKEGLAEHFAKRFGKVATGLFINTFSSLELR"
     gene            complement(19315..19989)
                     /locus_tag="PKZ49_02540"
     CDS             complement(19315..19989)
                     /locus_tag="PKZ49_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HND35468.1"
                     /translation="MKKIIKFVPLATIIMLSSCATISDTQRGTAQGAAIGAGAGAAIG
                     AIFGGGKGAAIGAGSGAVLGAGAGYIWSQRMEEQKKQMESATAGTGVQVSQTTDNRLK
                     LNIPSDISFDTGRDDIKSNFRPILDNFATSLLNNPGTSVMIVGHTDNTGTDAINNPLS
                     VNRAASTRDYLVSKGVPINRIQIDGRGSGEPVAANDTPANRAENRRVEIFVSEIQEEA
                     PPVSKP"
     gene            20081..20389
                     /locus_tag="PKZ49_02545"
     CDS             20081..20389
                     /locus_tag="PKZ49_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HND35469.1"
                     /translation="MLAVKLLFIDKEKEKSEKMTQSIIDCELHDYLEVACLYGYEVQL
                     TLKDQQIIQGKAKNIITTAEKREFLIVENATGLQQIELIQLQKLQVITPNAQFKEIIF
                     "
     gene            20810..21148
                     /gene="raiA"
                     /locus_tag="PKZ49_02550"
     CDS             20810..21148
                     /gene="raiA"
                     /locus_tag="PKZ49_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HND35470.1"
                     /translation="MNLNLTGNHVEVTPAMREYVVSKISKITRHFDHVIDVSVILSVE
                     KHKQKAEANVHVRGKDIFVEADGADMYASIDSLVDKLDRQILKYKEKNMERRNHDTIK
                     KKEIDQEPEA"
BASE COUNT         5905 a         4077 c         4811 g         6390 t
ORIGIN      
        1 accccgttca cctagaattt ttgatatttg aagatcgctg agtggtttct tttgattttc
       61 aacttgtatt aattgtttga ttaagcttcg aattgcaacg gctgagcagg tgtcaccggt
      121 atccgtcgcg acatggctac tgaaaaagta tttcagttca aaaataccat gcggggtatg
      181 gatgaatttt tgagtagtaa cgcgtgaaac tgttgactca tgaagctcga gtgattctgc
      241 aatttcgcgc atgatcagcg gtcgcataga aactgcacca ttttcgaaaa aatcttgttg
      301 ccgcgtaaca atttcgttgg atactctcag aatcgtatct gagcgctgct gaatattttt
      361 gatgagccat ttggcttcat tgagttggcc gatcaaagat tgagtggatt cattcttatt
      421 gtgcttgaga atatttgcat agagatgatt aatactaagc cgtgggagtg aactgggatt
      481 gagctttgca atccaggctc cctcggattt agcagcaatc acgtcaggaa cgatgtagcg
      541 agcaaaatca tttttatatg cttcgcctgg ccgtggattg agatgaatga ttagctgctg
      601 tatggcacgg aggcaatgat catcgcactg cagcattttc ttgatctgtc taaaatcctt
      661 cgctgctaga cattcgagat cagtcataac cagttgtaaa gctttatcac ggtaaggtgt
      721 tgtttctgga agtgactgta actggagcgt gagacattct cttaaattac gtgcaccgat
      781 cccaggtgga tcgagtcgtt gcagatgttt aagcgcagtt tcaagatctt ttagctgaat
      841 acccaattca gaaggaatga gttctgaaag ctccattaaa tcctgactta aatagcctgc
      901 atcatcaaga ctatcaatca gtagtcctac aattctccgt tctcgatcgt ctatttggct
      961 gagactaatt tgtgttgcga gatgctctct tagactaatc gactcagcag tctgttgaaa
     1021 taaatcccac tcgtcatcgt catcgcttcg tttagtatag gggctatctt gatcgccata
     1081 ccattcaagc tcaatttcag aagcgctact tggcgataat tcgttattat tgagatacga
     1141 atcttcactt tgaacgcctt cttcatttga aagtgttggt gaagattggc tttcgctggg
     1201 tgcatcatcc cgatagggct gttcatgaat cgtattttct aactctaaca agggattttc
     1261 ttgagccatg cgctcgatct cttgattcaa ttcgtgagtt gaaagttgaa gtaaacgaat
     1321 cgattgctgt aattgaggcg ttagcgttag attgggggtg agttttagct gtagtgttgg
     1381 tttcatgatc taaatttcac ttttcttagc ttattcatgc ctaaagccgg aaatgctccc
     1441 ctagataaac ttctctaact tgctcattgt tgatgatttc gtctggtttt ccactcgcta
     1501 gaaccgttcc atcactaatt atataagcac gatcacaaat accaagtgtt tctcgtacgt
     1561 tatgatctgt aatcagaacg ccaatgccct gatcttttag aaagcggata attttctgga
     1621 tgtcgataat ggcgatagga tctacccccg cgaatggctc gtcgaggagt acaaaattgg
     1681 gttgtgaagc taaggctcta gcaatttcta ctctgcgacg ttccccacct gacaaactga
     1741 tgcccatatt ttctcgtaaa tgactaatat gcaggtcatg tagtagatta tctaaccgct
     1801 gctgtatttc atccgcactt aaattctgta gttccagaac ggctagaata tttttctcaa
     1861 ccgacaagcg tctaaaaatt gaagcttctt ggggtaagta actcaatcct aagcgagctc
     1921 gctcgtgaat gggtaattta cttagattgc ggtcatccag gtagatttca ccaccacata
     1981 atggaactaa accgacgatc atatagaaac aagttgtttt gccagcccca ttgggcccta
     2041 ataaaccaac aacttcgttg ctagcgaccg agaaggaaac atctttgacg acggttctcg
     2101 atttatagct tttttttaaa tttttagcgc ttaagatgct catggataaa cgattgtctg
     2161 ttataaacta aaaaatctag ttttctgagt catcttctat ttttggagtc gctttgggtt
     2221 gaatgacaac gcgtacgcga cgatctgggt tctcaacctc gttcttcgta ttatttcctt
     2281 taacctggaa aaaatctcga gcaacatcat aagaaatata atccccttta acttcatctg
     2341 gacctcgctt taatcgcgct ttaccaaata attcgatttt gtcggtttta tcatcatatt
     2401 cgacacgttg gctccaggct tcgataaact catttgcgcc gtctcttttt tgtcggaaac
     2461 tgacgagatc gccatgggct gtcgcatagc gatagccttt tagatcttct ttcataatta
     2521 ttttatctgc tttaatcaca atggttcctt gggtgaggat gacattgcca acgaacgtgc
     2581 tgatacgaaa ggtgtctttg cgtttatagt cttcgaccgt tgcgctatca gattctaatt
     2641 gaatcggttt gttgcgatct gcgcgttcag caagcgccaa actagagggt aatacaccaa
     2701 cgaccatagc gagcaagcag ctaatcaaat gtacccgctt aaaagtggga taaatcaaag
     2761 tatcgcgatt acgcaagatc gatttcattt tttttgcttc atagtttgac atcccgcgcc
     2821 tttacttttg agtggagttc aatgatgccc gttttattat taagctgcat tcctactgaa
     2881 ttcacaatgg tattcatttt tgttatggtt accggccgat ctgtttttac gatttcttga
     2941 tcgggaacta agtgcagcga gtcagttacc atggtaacga tatctttgtt ggcttcatca
     3001 cctcgaacaa cattaacatt accaaataag taaatatctt cattcccttt ggaaagctcg
     3061 gctgaatctg cattaatttt taatgtcggt tcattggtct ggttatgggt aaagtgaacg
     3121 tgctcaagcg ctgaaacatt cgtcactgga taatgcttca aaatcttggc agtgaaatgc
     3181 cgctctgcag cctgcgcata gaccatctgt attcctgaca agttttcgat aacataatca
     3241 ggatttttgg tttggtcggt atcgtaacgt tttttaggtt gctgaatgga ttgatctagc
     3301 cagaatgtga ataagacaag aatggctaat ataattacgg ggccaggtaa taagcggcga
     3361 atcattttat tagtaattcg tgagaatgat gacttagatg aatagggttt aataaattaa
     3421 tctgactatt gatttaatgg ttataaattt taacaaaaca taaaaaaaat aactgtatga
     3481 gcagtaaagt aattaatgtt tatttaatgc atatcagtga agcaaatcaa ctgaattaag
     3541 tagtggagtt taaatatggg acaactaagc agtaaaaagc agtctttccg ctcgcttttg
     3601 tttactggat ccttatgaat ggtggatact attataacac tttgtaacct atcctgatta
     3661 ttagcgtatc agtactgatt gagttaattc aaaagtcagc gttggttgat tcgatacttg
     3721 tcgaagctgc acctggtagg ttgttacttc actcccttgc gagacgatat tgataaacgg
     3781 taagtttagt gttcccattg ctgagttaaa tattgcattg tcagtatcaa taatacgatc
     3841 tggtagcttt tctgcatgaa tcaattcaaa gcgtagcggt tgactatctg gaatgagccg
     3901 aaggcgagca tgataagttt cagctttatc gattgaagtg ataaccttaa ctgctggtat
     3961 tgcaacctca gttccatcaa aatgactatt gggaacaatt ttataaacga cccctgttga
     4021 tccaaagggg agtcctgtgc tatttccaag tacataaagt tcgccatcat tatcctgacc
     4081 aaaaccgagt atccagaacc caggttgcga ttgctgggtc aaatcgagtt cttgaatttt
     4141 actgccatct gagtagaaga gtctgccatc tccattgctt gttttggcga catcgccaaa
     4201 aacataatgt cctttcaact cgggtatcat tttaccctgg tagacaaaac caccgataat
     4261 cgcagtcccc tcgtcatgat cgtattccaa gattggatcg ataaaatcac caattatggg
     4321 ggcattggtt ataaaacctg ggccatttcc atttggttca aagcgaaagc gaccttcttt
     4381 tagtccccag ccataatttc ctcctgattt aacgatattc acttcttcta tggtgttttg
     4441 accgacatcg gctaatagta gagcacctga tacggaatca aatgaaaagc ggaacggatt
     4501 gcgaaatccg taagcataga tttcacccaa cgcattatta ctgttagaaa aagggttatc
     4561 atttggaatg ccatattgcc cattgattga attggttcca ctgggatcga ttctgagtag
     4621 gctacctaac ggattggtta aatcctggct attgccatga gtgccatgcc caatgatggg
     4681 ttccccgata aaaggttgtc catcgcggtc atcggcgccc ccgccatcgc caagggcgat
     4741 ataaagcatc ccatctggac caaaatttaa tgcacctgca ttatgattga attgcggttg
     4801 atcgatactc agtaaaaccc gattgggcat atcctttgct aaattagcgg agttgattct
     4861 ccattcccgg atgacggagc ggtgattggg gtttgagcca gaagagatcg ttgagaaatc
     4921 acttttctca cccgctggct ccgaagtata agtgtagagc aagctatttt gcgtatattg
     4981 agggtggaag gcaaaaccga gaaaacctct ctcgtcaaat gaattttcac caaatatgcc
     5041 gagtggcacg agtagttgag aaagatcaag gaaactttct ttgttgccat caagcaagtt
     5101 gattttccat agctttccat tttgatcacc tacatagagg tgatcggtat cccctggtgc
     5161 tgggatagcc caattgggtg ccgtcaaccc ttgtgctatg agttttagtc tcagacgtag
     5221 atggcctttt aaaatcggct caggaattgg atcagttagc gtttcttcag tagtgatggc
     5281 taaatcttta gaaatgttta ctgggttagc ctgtatttcc atgctatacg tgccaatagc
     5341 taaaaatagc aacgataatg tgctgcgtat cataatactt accccccttg agcggataaa
     5401 cgcttactca tcgatcatac tccagtatag atgaatccat acttatttat tttatttcat
     5461 agagttattg cttgaaattt ttttgactat ctggataatg ttgcatatga catatttttt
     5521 atggaaacat gctgtatgaa agctactgta actgaacttt tgcaacgtat gcctggaaag
     5581 gtttctgagg aatggccaat gggggagcct tttgccgttg cacttgcaca tggttcgatg
     5641 tcggtagagt tatatgcgcc aaaggatgtc gatattcaaa ctccacatga tcaggatgaa
     5701 ctgtatttta ttcattccgg aaaaggtgag atcgtgattg cggggaatcg ctacgcattt
     5761 gaacctggta cggttttctt tgttccagcg catattgagc atcgttttga aaatttttct
     5821 gctgactttg caacctgggt tgttttctgg gggccnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnatcgt tttgaaaatt tttctgctga ctttgcaacc tgggttgttt
     6001 tctgggggcc caaaggggga gaaaaagcgt aatgggtgtt gcaaagaaaa ttttacctcg
     6061 atcgaacgtc aaaggaggcg tcatgttcca aagaaaaata atgctagtca caatactgtt
     6121 cacttttttg ttggtagatg tgacagcagc gccggttccc gttggtccag cgctaatggg
     6181 aagtggggat gggttaaaga tgcgttgggt aaatacggat tttagcccgc actcaataac
     6241 agatgctgct caagccctta atctcaggcc aggtgatgtg ggctatgtgg gagaagtcag
     6301 cggcgtcaca aaagtcatcg actttgaaga cggtaattat ggagatttat ttcgggatac
     6361 tgaccaggcg gtcccgctcg gcgctgatga taattttgct gttctttata aaggttttct
     6421 gaatgttact gtgagcggta actatacatt tcgcagtttt accgatgatg ggtttcgctt
     6481 aacgattggt ggagaagtag taagtctttt taatagtgat cgtagccctg gtgaaacaac
     6541 tacgagtgta tttttaaatg aagggcttta tcattttgaa ttcattggtt gggagcaagg
     6601 ggggcaattt gtaaatgagc ttgcttggat caaacctgga gaagttgagt tcacccaacc
     6661 tggggatcgt actaacttag tattttttac gacgcaagga gatgcttctg ttccagagcc
     6721 gatgatgctg agtttattct tgatagggtt aattgtaatg ggatacgcaa ggcaatctcg
     6781 ccaatcctga ggttttgttt gattgatgag ggtaataatg aagctaccct cttgtaatac
     6841 ttaacgttca gtcaacatga tatctacggt tattgttttt ccatcacgca taaactgcaa
     6901 ggtggtttgc tggcctggtt caagtgtttt taaaatcgct gaataagttg ccagatcgct
     6961 aatcggttga ttggcaagtg caatgagaat atcaccgtgc tgtagtggga ttagctcagc
     7021 aggcgaacct ggcatcacat tctctacccg aaccccttta ccttggtagg aaaaatctgg
     7081 aaccgtaccg agactgactt tgcgtttctc agtcatgggc ttgatttgag ctctgctttc
     7141 agtagtttga atggtcaccg tgagtggatc gtgtcgattt gcgagatact cggtagcttc
     7201 cttgagtaca ttcgcgactt tgactaagcc cgaagcatcg attttatcaa cggtgtcacc
     7261 gggcgcatga taatcttcgt gggcgctggc aaataactga atcgcgggaa tgcctgcctt
     7321 gatgaaggca gcttgatcgc tggagccaaa atcatcgagt accgagttga taggaatgcc
     7381 agttacaaaa ccgatacctc ggaaaatatg cacaaattca cgtgcggtac cggtaccaaa
     7441 gatcgttacc ggattgttac caagccttcc aaccgtatcg aggttgagca tggcgatgat
     7501 tttttttgta gggtagttgt tatcatggtt tgcgtaataa gttgagccca gcaaattcgc
     7561 ctcctctcca gtaaatgcaa taaaaatgat tgttcgctgc ggttgccatt ttggggcaat
     7621 ttgtctggca agctccagca ttatggcaat accgctcgcg ttatcatcgg caccatagtg
     7681 aattttacct tggttgtcgt gccgaacatc aggccaaccc atcccaagat gatcatagtg
     7741 agccccaatc acaaggcttt ctttatctag ttgtgggtta gatcccggca atattcccac
     7801 cacgtttcgt aaggaaacga tacctttagg tggaccaaca tcatgttgcc aaacttggaa
     7861 aaaacgctga ttgtcaccac cgggtagcaa ccctgcctgg tgaaactgct gtgcgatata
     7921 gtctgcggct agatccaatt cgggggttcc aagctctcgc cctttaaagg ttgcgctcgc
     7981 taagtgcgtg atatcagcta gcattcgtgt ttcagagaac agtggaggta attctgcaag
     8041 ggtgcgacgg ggtgatagtg agccgacatg atcagatgcg aaagaaggat catcttgctg
     8101 gtggattttt tgcgtaagcg gtgattgcgt aattggccat tgtcctttat caatattcgt
     8161 taattcatcg cctttgaata ccaagaaact gtatttacgg tagtggggta attttcgggc
     8221 tagttctgca atcgcactgg gatgatgtgc ggcaacccat agtagggttt tgtctgcatt
     8281 ttctggttgc cttgcagtta atacgattgc gtgctcagcc tgatgatagt gttgctgggc
     8341 aatttgtaat gtattcgctt gataggaaac cttttgtgat tcgagcgtag acagcacggg
     8401 ttgactaaat cgattctccc atcctaatac ccatatggtg cgatcgactg gcagtttgtc
     8461 gagttgatga tcttggatga tttcgagtcg tattgattgg gttttctgcc aattagcagc
     8521 taattgctga taggcctgca gcaaagcagg atcagccttt gaaggtaata tcactaatgc
     8581 gttttctgca ccaaatccct gagaaagtgc tgatggaatt tcacggctat cgagtcgtct
     8641 gaatacatca aattgtggat cgatatcgat tcggacgggg cgctgcggta aatcaatcga
     8701 gatggtttga acggtttggt taagtttgat cgaatgctga tacgcttgtg gttgcccctc
     8761 aagatgaata gcaatcggta gcgttaagtc gtagggtttc cctggttgtg tttgtttaag
     8821 cgtcactttg agtctaaagc cattagcagc cggttcggct tgagcttctt ggaggtgtag
     8881 attgggcgcc ccactttggt tgatccattg cgcaaaaaat ggcgtaaagt cactgcgggt
     8941 atagtggttg atacttgcta gtaaatcctg aaatgttgct tgtttgaatt tgtattgctg
     9001 ataaaaagca cgcaatagac gaacaaaatc gtcatcccct aatgtttgac gcagcatatg
     9061 gaaaaacatc atggttttac catagcccac agcttctgag ctagagctat ggcgcgaagt
     9121 aaactgtttg atggggaaat ctttttcttt attaacgaag tcagtgtatt tttgcagtac
     9181 atcgcggcga tattcctcgc cttttctgcc ttgttcggca actagatgat ctgcaagata
     9241 agcagttaat ccttcagacc aatttccttg agtgtaatcg acgaataccc cgttacccca
     9301 gtagttgtga agaatttcat gggggtaaga cgtgtgcaaa ataaaaggaa agcgaacgac
     9361 tttggggcct aataaagtaa aagatggcat tccatacccg gtctcccaga aattttcaac
     9421 cagcgcaaat ttagtgtaag gatagggtcc gagtaacttg ttatacatcg cgatatattg
     9481 cgcggtggta tccaaatatt tttgagctaa tgctttatcg gagccacgta gataaacata
     9541 ggaagttatg gtcgtagtgg ctggctgggt atagcgttca aagcgacccg cgattaggta
     9601 gatatcgtct tgggggtgtt tttcttccca aaccacttgt tgcgtggtgc ttgatttttg
     9661 ctcacttagc aatgtacctt gactgacagc atcccagtct tgtggtactt tgacttcaag
     9721 ctgaaaagaa accatcgcgt tttcaaattg gggataccag gctgttgaat ttgctagaaa
     9781 gacaccctct tcagaaatga gacctggtgt gtagctaaaa ctgcgtgcat attcttcacc
     9841 agggccttgt acggtgtggt gaatctttcc ttggaacgat aactgaaatg agttttgttg
     9901 cgcggggagg gtgacagtat aacgtttgac tggaacaggt ctcgcgagca gcgtattaat
     9961 cttttgttgt ggagaaagct tagcaccatt catttctgtg atggataagt cagcatgcaa
    10021 gctaaaatcg agctttgttt cgctatctga tttcaataag tgcttaggta cctgtattgt
    10081 gctggtgact tgaatttcag aagcgctagg tatcagggtg atttgcattt gatgatgaaa
    10141 ggtctcatcg ctcgcactag cagacattgc gaaaaggcta taaatcatcg tgaggcaaat
    10201 ggctactatc aggcgagtct cagttttcat ggttttattc tcttgttcga tcatgtcgtg
    10261 cattttggca gatagttatg gttattggga atagctttga aactaagctg gcaggcttac
    10321 tttctttttc aagctatgtt taaatggatc aaattaattt gtgaggttta tcagtcctat
    10381 ggaaaaacac gttcaatcat ttaagcgatc aagctggtca attggatttc tgagtttagc
    10441 gttactttgc ttggcaatga gtgttactgc ttccgatttt attgtggaag atcatcaatt
    10501 gtcggataaa gaggtgagag ttggagaagc ctactatcgg gccgatatga aatggatggt
    10561 ctatcaagca gaagtgtctg aaagtaaccc gttttaccag atttatttaa aagatctggt
    10621 cagcggtaag gttcagcaag tctctccagg ggatgggaaa acgacttgtg catgggttca
    10681 tccatcgcaa gagaaagttt tattttcatc gacgcacgaa gattcagatg caaaagcaaa
    10741 aatgcttgct gagatcgaac ggaggaaatc aggtgagaag caatcttatg cctgggatta
    10801 cgatgaatat tatgatattt acgaaaccga ttttacgggt ggcagaatca agaatctaac
    10861 gaataccctc ggctatgatg ccgaagcttc ttggtcaccg gatggtaagc ttattgcatt
    10921 tgcttcgaat cgcagggctt atactgaaaa attatccgat gaggaagcag tattattaaa
    10981 aaaagatcct tctgctttca tcgatattta catcatgaat gctgatggct ccaatgtgaa
    11041 acggcttact cacacgaatg tctatgatgg agggccattt ttcagccccg atggaaaaag
    11101 aatcgtatgg cggcgattct caaccaatgg tcgagaagct gaaatcttta caatgaatat
    11161 cgatggatca gaccagaaac aaattacgca cttaaacatg atgtcgtggg cgccttttta
    11221 tcatccctct gggcaataca tcattttctc aacgaatctt catggtcatc gtaattttga
    11281 gctttatatc gttgatgtcg acggaaaaaa acaaccggtt cgtgtaacgg ataaggatgg
    11341 attcgatggc ttacctgttt ttacaccgga tgggaaagcg attacttgga caagcgaccg
    11401 tacacctgag aaaaaagggc atttgttcca tggaaaatgg gatcatacaa aagcactgga
    11461 aagcctttcg ttgcagtaag gttgagtcag aattgtaggt taattggttg ggttgttgtt
    11521 gcgtatgaaa ttcatacagt tagctttgat ttggtaataa aatgagcttg tgaaggtttt
    11581 tctcaataac aattcatagg ggattgatat gtcaattgca aaagtttttg aaatcatttc
    11641 atcgagtaaa gtgagttttg atgatgcgat taagaaaggg gttgcccgtg ctgcagatac
    11701 tatgaccgat atcacctcag cgtgggtgaa agatcaatct gtagaaatct caggtggaaa
    11761 aattgttgag taccgcgtcg tgttgaaaat aacctttgtt cttaaaccag cgcctaaagc
    11821 tgcggtatct aaaaccaaag caaaagcaac caagtaattg gcacaatgat actatcaatc
    11881 gattagctgg gtagtgggct gggacttatt ctgccgtgtc atcgataact atggtgatat
    11941 tggcttttgt tggcgattat cacggcagtt aacaacagaa tatggcttag ctgttcgatt
    12001 gtgggtggat gatttagcga gtctgcagcg catttgcccg agtgttgatc cacatcaaac
    12061 gtttcaacag cgttgcggtg tgcaaatcaa tcattggact gatccattcc cagatattat
    12121 tccggctgat gtggtgattg aggcttatgg gtgcgagctg ccggaaaaat atatccaggc
    12181 aatgcttccg agtcattctc gaagccgtca accggtatgg atcaacttgg aatatctatc
    12241 tgcagaatca tgggtggagg aatatcatgg actttgctca ccgcatcccc gtttacctct
    12301 aacgaaatat tttttctttc caggatttac agcgaatacc ggtggattac tgcgagagaa
    12361 agatctacta gtgcaacgcg atagcgtgca acacaatgca gctaattggt ggttgcaaaa
    12421 tgatttacct attcctgatg aaaccgagat cgtcatttct cttttttgct atgacacagc
    12481 gccgatttct aatttgctag aagcttgggc tcattcgcag actccgattc gctgtgttat
    12541 ccccgaaggt caggcgttga ctctactcag aaatgggtta aaccgatcag cattaaaagc
    12601 gggagatgtt attcaacagg ataaacttac cgtgcaaatc attccatttc tacctcaagc
    12661 gcaatatgat caattactgt ggctatgtga ttgcaatttt gtcagagggg aagattcatt
    12721 tgtacgagct caatgggcgg cacgtccctt tgtttggcaa atttatcccc aagctgataa
    12781 tgttcactat gttaagcttg aagcatttct taatcgatat tcacagtcat tgaccaaaga
    12841 gtctgcccag gcactccggg ctttccatca tggttggaat ggaagtgagc gtctcgattg
    12901 gaatcatttt tggcaacacc atgcaatttt ccaccaacat gctttaactt ggtctagcca
    12961 gttagcagta attcctgatt taacctttaa tttggtcgaa ttttgtaaaa gacaataaga
    13021 ctaggtgctt aggaagcaaa atggttagcg ctacaggaaa acaatgaatc gatttctacg
    13081 aagaaaccag accatcatct tgatggaatt ataatattaa gaatctaata gattgcggtt
    13141 gttagcaaca atttagatga gaatcagctt tgattttggg gagttaaaac tacgaatgac
    13201 aggacgcaca caggcgacag ctttacgcgc gatttatcaa cttaaagtta ccttgaatta
    13261 tattcgtcca ccgatatggc gcaggttttt gattgccagc actgacagcc tagcaaattt
    13321 acatcttgtc gtgcaaatcg tcatgggttg gagcgattcg cacttgcatg aatttgcaaa
    13381 aggtggagaa cgctatggcc tacccgatga agattttcct tcagcgatac acgatgaatc
    13441 caaatttcgt attagccaga tattgaaaca ggaaaaagac aaaatccgtt atacctacga
    13501 cttcggtgat aactgggatc atgaaattgt tctagagaaa atcctaccct ttgaaatggg
    13561 aactaaatta ccaacctgtc ttaaaggaag tcgtgcctgc ccaccggagg atgttggtgg
    13621 cccgcctggg tatgaaatgt tccttgaagc cttagctgac cctagtcacc cggagcatgg
    13681 cggtatactg gaatggttcg gtgaaccgtt cgacgccgag cattttgatc taacggaagt
    13741 gaatcactta ctgcacaact aaaagtgtga acggattact catggattca tccggtttca
    13801 aaaatagcga tgatggtttt taaaatttta ggcaatttac ccaaaaattg aaattcttgt
    13861 tctggcgtgt ccatatgcaa ttgctggtaa agtgttcttg gtaaatgctg tatcatttcc
    13921 tactttaaga tggaataaat tttagttttc atgaagacaa cgtttttaga ttttgagaaa
    13981 ggaattgaag aatttgaaga gaaaattgag caattacggt ttgctcaaga taactcagct
    14041 ttggacattt ctgcagaaat tgctaaattg caagcaaaaa gccaggcact gactaaaagt
    14101 gtctatgcaa aattaacacc gtggcagatt tcacaagtcg ctcgtcatcc acagcgccct
    14161 tatgcgctgg attatattca gcatcttttc acggattttg aagagctgca tggggatcgt
    14221 aattttgcag acgatcatgc aatcgtgggt gggctggcac ggtttaatgg tcaatcgatc
    14281 atggtgatcg gtcatcagaa agggcgtgat acgaaagaga agatttatcg aaattttggt
    14341 atgccaaaac cagaagggta tcgtaaggcg ctgcggttga tgtcgttagc tgaaaaattt
    14401 tcaatccctt tgattacgtt tatcgatacg ccaggcgctt atcccgggat tgatgctgaa
    14461 gagcgcgggc aatcagaagc cattggaaag aatctctatg taatggcagg gttagaagtg
    14521 ccgatcgttt ctgtcatcat tggcgaaggt ggatctggtg gtgcactggc tgtagcagtg
    14581 ggtgatatgg ttttgatgat gcaatatgcg acttattctg ttatttcgcc tgaaggttgc
    14641 gcctctattt tgtggaaaag tgctgagaaa gcgtctgaag ctgcggagat tatgggaatt
    14701 accgctgatc gattgaaaga gatgtcatta atcgatggca ttatcaatga acctattggg
    14761 ggagcccatc gtgactatcc agccacaatg ctatccataa aaaatgtatt acaagaatcc
    14821 ctaagaaaac ttcaagatat atctattgaa agcttattag aacggcggtt taatcgttta
    14881 atgggacatg ggaagtataa ggaagtcaag cctgaacggc atacgtgata cggttgaagt
    14941 cgttttacgg caatatgtgt ccgagcaaaa tcacctagcg cttgctttga gtggtggcgt
    15001 tgattcagtg gttttactgc aaattcttgc tcagatttca aaacaattta cttttaatct
    15061 ttctgcgatt catgttgaac atggcattag tcctaatgca aagcagtgga gcgatttctg
    15121 tcaaagttat tgtgatgcgc ttggggttcc actgaccgtt tttcatctga gtatcaagaa
    15181 acaatctcaa gtaagtttgg aagcagcggc gcgacgcggt cgctatgcta tatttaaagc
    15241 gatatcagcg gattttattg tactagcgca tcatttagat gatcaggctg agacactcca
    15301 attgcaattg ttacgtggcg caggactgag aggattggct ggaatgccta ttatgaggtc
    15361 acttggttca gcggcgaata atgtaaaact attgagaccc ctcttaactg tttcgcgcga
    15421 tattattcaa gactacgcct tacaaaataa attaatgtgg gtaaccgacg aaagcaatga
    15481 cgatacgacc tatcatcgga attttctgcg ccaccagatt tttcctgtat tagagcaacg
    15541 atatcctgct tatcgagaaa cattagcgcg aacaagtcga catattggtg aagccattca
    15601 attattggat ggtttagcgg aaaatgacag ccaatcagtc atggtgatgg gagatatcca
    15661 cattgaacga ttacgcaagc tagactttct gcgagctaaa aatttactgc gttatatttt
    15721 ttcacgacac gatatcattc agccgagcac tcaaaaatta gaagaaattc tacggcagtt
    15781 gtgggcagtt aaatcagaca atcatcttca tttggtattt ggggaattcg agattcgctt
    15841 gtataaaaat ttagtcaaac tgctaccaaa agccaaaccg cctgatttcg atcaagatca
    15901 actcttttac tggcatggag aactgaactg gctaatcgaa ccattgggtg gtgtgattaa
    15961 atggtcaaat agtacgggaa ctgggataaa tgctaaaaag ttaatggaaa atccggttgc
    16021 tgtgcgtctg agaaaagggg gtgaacgatt tcaaccaaat tgtaggcgtc ctcgacggag
    16081 tttgaagaaa attttccagg aagcggcaat accagaatgg gagagaaatg atttaccatt
    16141 gttgctaagt ggcgatcagc ttgtctgggt tgcaggaatt ggtattgatt gtgggtttca
    16201 ggttatgcca ggtgagcaag ggctaatgcc ctcatggcat cccagttaaa aattttagag
    16261 gtaaagagaa atttatgatt ctggttacag gtggtgcagg ctttattgga gcaaatttta
    16321 ttctggattg gctaagccgt attgatgagc ccgttgttaa tttagataag ttgacttatg
    16381 caggtaattt gcaaaacctt atttccttac agagtgatgc tcgtcatatt tttgtacaag
    16441 gcgatatctg tgatggacag aaattgaatc aactgtttga taactatcaa cccagagcgg
    16501 tgatccattt tgcagcggag agccatgtcg atcgttctat ccatggacct gatgatttta
    16561 ttcaaaccaa tatcgtcggt acttttcgtt tgctagaggc agctcgccat tactggaata
    16621 agctaacagg aaaggcgcat tacgattttc gttttctaca tgtttcgact gatgaggttt
    16681 atggttcact cgcaccggat gtacctgctt ttacggagaa gcatccttat agccccaata
    16741 gtccttattc tgcaagcaag gcttcaagcg atcatctagt tcgggcttat caccatactt
    16801 atggatttcc gacactgacc acgaattgct caaataacta tgggcccttt caatttcctg
    16861 agaagctgat tcctctcgtg attgtgaatg ctttagccgg gaagtcattg ccagtctatg
    16921 gtgacggttt gcagattcga gattggcttt atgtgaaaga tcattgtagt gctatttcac
    16981 gtgtcttgga ggcaggggtt ccaggagaaa cctacaatat cggggggtgg aatgaaaagc
    17041 caaatattga aattattcgt ttaatctgtg atttactgaa tgagttttgc cctggagatt
    17101 atcatcgatt gataacatat gttaccgacc gaccgggtca tgatcggcgc tacgcgatcg
    17161 atgcacagaa gattgagagc gaactggcat ggaggcccct agagacattt gaaactggaa
    17221 ttcgtaaaac ggtgcaatgg tatttagccc ataaagattg ggtagccaac gtacaatccg
    17281 gtgaatatcg aaaatggata actacacatt accaacagta aattaaatga aaatcctgtt
    17341 atttggaaag aatgggcaag taggttggga attgcagcgt agtttagcgc ccttaggtag
    17401 ggtaatcgca ttggatcgat tcagccaaga atattgtggt gatatcacgg atacagcggg
    17461 cattactcag ataattaggg caatcgcacc ggatgtgatt gttaatgctg ttgcttatac
    17521 tgcggttgat aaagcggaaa gcgagccagt tttagcacgg caaatcaata ctgaagcgac
    17581 tgctgttctg gcacatgagg caaaacgtgc tggcgcttgg ctggttcatt attctacgga
    17641 ttatgtattc aatggaagtg gaaacaaacc ttggttagaa acggataatc ctgcaccact
    17701 caatgtctat ggtcagacaa agctagaggg tgaagaagca attcgagaaa ccggctgttt
    17761 atatctaatt tttcgaacga gctgggttta tgcggtacgg ggcaataatt tcattaaatc
    17821 gatattacga ctcgcgaatg aacgggatca actgacggtg attaatgacc aaattggtgc
    17881 accaacgggg gctgattatt tggcagatac aacagcgcat gcaattatga tggcgatcaa
    17941 tcgacctgag ttatcgggac tctaccatct tgttgcggaa ggtgaaatct catggcatgg
    18001 ctatgccagt tttattatta attttgcgca agcacagggc atagatttga agttgaagac
    18061 taatgctatt gttcctgtac cggcaagtaa ttttaagacg ccagcaaagc gaccgtgtaa
    18121 ttctcgatta agtacggata aatttcagaa aacgttccgc cttaatgtac ctcaatggca
    18181 aattggagta tcgagggctc ttaccgaaat cctagagcgg taaactttgc cagtcataaa
    18241 aatactcagc gtaattccaa actactgaat gtattgataa ataggcccgt tgcaactttc
    18301 ccgaaccgtt tggcaaaatg ttcagccaga ccttcttttg tggtgtaatc gataatacgt
    18361 tctgctttga tgatttcgcg agccacatat tctgtgttac ctaactcatc agttaagccg
    18421 agatcaatgc ttttttgacc tgtccaaacc atcccactaa aaatttcggg tgaatctttt
    18481 aatcgatcac ctctgccatg ctgaacgata cgaatgaact gctcatgaac ttcgccaagc
    18541 atttttcttg catgctctct ttgtttgggg tcagatggtg agaacggatc gagaaagctt
    18601 ttattctctc ccgcagtcaa taatcttcgc tcaacaccta atttttctaa ggtaccagta
    18661 aatccaaagc tatctaacac aacaccaatc gatcctacga tacttgcctt atccacaaag
    18721 attttgtcag cagcgaccgc aatataatat ccaccggaag cgcaaatatc ttcgacgact
    18781 gcataaactg ggatttgagg atgtttagtg cggagtctgc ggatttcatc attgatatta
    18841 ccagcttgta ctggacttcc tcctgggctg ttgatgcgaa taatcacacc agccgtattc
    18901 ttatctttaa acgctttttg gagacctgca ttgacgttat ctgcgctact gaggctgtca
    18961 gccatgattt ctccgcgcac atcgattaat gcagtgtgac gatccgacga gctggtggaa
    19021 tcatcgctaa atagccccat tccccaaaac aataatatga aaagataagc gaaggctaaa
    19081 gatctaaaaa agatgctcca aagtcgcgtc cgacgttgtt cgcgtagcgt tgataacaca
    19141 actttttcta gtaattcttt ttcccaacct tctttttgat ttggtaactc tgtcatagcg
    19201 tcttatagaa taaattttga gtaaatgatg ctactgatcg gggatagatt atctcccaat
    19261 gaaacagcgt tgctattaac gagcaaccct gcttttttgt gttaattata ccgactaggg
    19321 tttagaaaca gggggcgctt cttcttgtat ttctgaaaca aaaatctcaa cgcgtctatt
    19381 ttctgcacga ttcgctgggg tatcattggc agcaacgggt tcaccggagc cacggccatc
    19441 aatttgaatg cgattaatcg gtactccttt gctaaccagg tagtcgcgcg tacttgcagc
    19501 acgattgacc gataatgggt tattgatggc atctgtgcca gtattatcag tatgtccaac
    19561 gatcattaca gaagttccgg gattattgag taacgatgtg gcgaagttat ccaatatagg
    19621 acgaaagttg gacttaatat catcacgtcc agtatcgaaa gaaatatcac tgggtatatt
    19681 caactttaaa cgattatccg tagtttgtga aacttgcacg cccgtgccag ccgttgcaga
    19741 ctccatttgt ttcttttgtt cttccatgcg ttgagaccaa atatagccgg caccagcacc
    19801 gagtacagcg ccggatcccg cgccgattgc agcaccttta ccgccaccaa aaattgcccc
    19861 gatggctgca ccagcacctg caccaattgc cgcaccctgg gctgttcccc gttgagtatc
    19921 tgatatcgtt gcgcaactgc tcagcatgat aatggttgca agcggtacaa atttgatgat
    19981 ttttttcata aattgctcct tgataataat ttgtactgta gcagatgaat caaaaaattc
    20041 aatgttagag actgcttaac taattttcta caattctaga atgttagcag taaaattact
    20101 gttcattgat aaagaaaagg aaaaatcaga aaagatgact caatccatca ttgactgtga
    20161 actgcatgat tacctggaag tggcttgctt gtatggttat gaggtgcagt tgacattaaa
    20221 agaccaacaa ataatccaag gtaaagcgaa aaatatcatc actactgctg aaaagcgaga
    20281 gtttttgatc gttgagaatg caacaggatt acagcaaatt gaactcattc agttgcaaaa
    20341 actgcaggta ataacaccta atgcacaatt caaagaaatc attttttaaa ccaattcctt
    20401 tttaggggtc gtatcaaggt tagtgtgtat tgtataatcg ctaattgaaa aaaattagtg
    20461 tgtattgatt tgagttaaaa aattgaagcg ttacaaataa tacaattaac cattcgattt
    20521 tccgttaatc tgaaataaat tggttggatt tgttttctaa tgatcagttt ttattataga
    20581 tctgatatcc caaagttctg ttgactatct tgagttattt ctataaatcc ttacttaagg
    20641 ccaataataa aataattaat atagcgaata gactcaattc gatatccatt atgaattcat
    20701 ttaccgtttt tttcttgaaa tttgaataga aaagacaagg caaggatgtt catgggggcc
    20761 tgtcaattta gctgagaaca atgaaattac ttaatcgaag ggggaaatga tgaatttaaa
    20821 tcttacaggc aatcacgtag aagttacgcc ggctatgcgt gagtacgttg tttcgaagat
    20881 aagtaaaatt acgcgacatt ttgatcatgt aatcgatgta agcgttattt tgtcagtaga
    20941 aaaacataaa cagaaagcgg aagccaatgt ccatgtccgg ggtaaagata ttttcgttga
    21001 agcggatggc gctgatatgt atgcctcaat agatagcctg gttgataagt tagatcgaca
    21061 aatccttaaa tataaggaaa aaaatatgga gcgtcgtaat cacgacacga tcaagaaaaa
    21121 agagatcgat caagaaccag aagcgtaatt accaatcgat ttttttaatg atttcatcga
    21181 tcagattgat cttagactga tcagtaagtg gtagttgttt aaagtaaccg tttatttttc
    21241 ttatgaatct tgtttctcag tttttacctg aatcgaatgt aat
//
LOCUS       DAOQKQ010000022        21095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_1087_length_21095_cov_5.185361, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000022 DAOQKQ010000000
VERSION     DAOQKQ010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 21095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21095
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1087_length_21095_cov_5.185361"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1231)
                     /locus_tag="PKZ49_02555"
     CDS             complement(<1..1231)
                     /locus_tag="PKZ49_02555"
                     /inference="COORDINATES: protein motif:HMM:NF016635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HND35471.1"
                     /translation="MSQITVEQFANELGMLPDALLEQLQTAGVSKQSGEEIITEGDKK
                     QLLEYLRKSHGAAETTKKKIALTRKKTTEIKKSDVAGKQRTIEVKVRKTRVYTKPVEE
                     TVEQSEDKETKAAIAPIEVSVSVPETSSVRKPIISADELAQREEEARRHAKLIELQEI
                     EFRKKAEKKIQPKVSEVIKEEPKQEVPVEKVTNTAELASQPTAQQPKSTITVKHEEKP
                     EKKKKQIKQQNLLSEETGKRREIKKHDLNQADGWRIRKTNKNAKASSNESAELHRFTE
                     PTEPIKYEVMVPETISVAALAQKMSVKAAEVIRVLMKMGIMVTINQMLDQDTAMIVVE
                     ELGHIAKYAAPDNPESYLEEEKASQSDAIAEPRAPVVTVMGHVDHGKTSLLDYIRRTR
                     VAGGEAGGITQHIGAYHV"
     gene            complement(1340..2812)
                     /gene="nusA"
                     /locus_tag="PKZ49_02560"
     CDS             complement(1340..2812)
                     /gene="nusA"
                     /locus_tag="PKZ49_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HND35472.1"
                     /translation="MSREILLLVDALAHEKNVSKAVVFTALEMALASATKKHFREDIE
                     VQVRIERDTGNYRCFQSKQVVPDDALENPFRQVILSEALKLNAEIKLGDFIEEPIETL
                     EFDRIGAQAAKQVIFQKIRDAEREQNLNDFLDRKEYLISGTIKRMERGNAIVESGRIE
                     AILPRDQMIPKENLRVGDRVRGYLQRVDRTSRGPSLVISRISPEFLIKLFQLEVPEIE
                     EKLLEIKAAARDPGSRAKIAVKSNDPRIDPIGTCVGMRGSRVQSVITELTGERIDIIL
                     WSEDPATYVVNALAPAEISSIVVDEDKHAMDIVVNDENLAQAIGRGGQNVRLASELTG
                     WVLNIMTEEESQKRNEEETSVISKLFIDKLDVDEEVAAILVEEGFSTLEEIAYVPLEE
                     MLEIEALDEETITELRHRASNALLTAAIATEERIERVSEDLLALEGMDIQLVRELAAK
                     GINTQEELADLAADDLVEMTGIDIERANQIIIKAREPWFV"
     gene            complement(2840..3268)
                     /gene="rimP"
                     /locus_tag="PKZ49_02565"
     CDS             complement(2840..3268)
                     /gene="rimP"
                     /locus_tag="PKZ49_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HND35473.1"
                     /translation="MALYELLDSTLNNMGYELVDVEFSPSGKLLRVFVDKEGGISIDD
                     CVAISNHLSRLLVVEQIDYGRLEVSSPGLDRPLRKERDFVRFLGRLVKVKSHIALQGQ
                     RNFVGILREVSDGKLGLEIEGKILHIELNNLDKARLVPEL"
     gene            complement(3500..5680)
                     /locus_tag="PKZ49_02570"
     CDS             complement(3500..5680)
                     /locus_tag="PKZ49_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HND35474.1"
                     /translation="MLHFSLFNRTPVILQTEAAECGLACLAMIASFHGHQINLNGLRA
                     QHAASLRGSTLADLIQIAEQLKLASRPLRLDLDQLAELKLPCILHWNFNHFVVLTRVQ
                     RDRVVLHDPASGECELTLAEFSKHFTGIALELTPTSDFVTRNEIQRVKLRSLIGRLRG
                     LTGAISQILILALILQVFAILAPFYMQWVVDHALVAYDHDLVTILGVGFLLLAVVQVS
                     VTALRSWVLMVLSTTLNLQMMTNLFHHLIRLPMQWFNKRHIGDIVSRFDSLNVIQRTL
                     TTGFLEALIDGVMVLITLGMMLLYSVKLALIAMAAAGLYGLLRLVLYRPLRLATEDHI
                     VRGAKQQSHFLETVRGIQSIKLFAYEASRAANWQNLVIDQFNASIRTQRFNILYQGLN
                     GLLFGLENIITIWIGAMLILDTATGTGFSIGMLFAFIAYKTQFVQRISALIEKGLELR
                     MLSLHTERVGDVALTPIEQVNELHPISATPLTGRIEVKHLTFRHSETDPLVLQDIQFS
                     VQAGESVAIVGPSGCGKTTLVKLMLGLLQPTGGQIEIDGVPLSRMSLARYRQAVASVM
                     QDDQLFAGSIAENICFFDATPDFERVMASARLAAIHDDIMAMPMQFNTLVGDMGAVLS
                     GGQKQRVLLARALYRQPAILFLDEATSHLDIHCEQNVNQAIHTLKLTRIIVAHRPETI
                     ASADRVIRLVAGRIVSDTIVPAMANYRSESQLNELESQYVIAAN"
     gene            complement(5683..6930)
                     /locus_tag="PKZ49_02575"
     CDS             complement(5683..6930)
                     /locus_tag="PKZ49_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HND35475.1"
                     /translation="MARPLFRSEAIAHQQDTRQGEVLIARSLTIRLFVWVALIFTLGL
                     LAFAYWGEYTRKTHVVGYLSPTTGMIRIYSPQLGTIQEKHVREGQVVKQGDPLLTISS
                     ERTTSNAREAQAAMLTELKQRQVSLRFEQGKQSEIDALTGTSINARIRGLENEIRETQ
                     AQLALQSSRVASAERMVVRYQQLVAERFMSEVALQEKQEALLDQRNQLVQIKRTIASL
                     NRELNTARSELSASGLKKVNNRAAIERQISELEQQLTETDLRRSIVLTAPTEGIVTTL
                     LAEMGQMAQPNLPLLSILPAGAQLQAQLLVPTHSVGFIKPGQMVSLRYQAFPYQRFGH
                     HLGEIIEVSRAVIQPNEANLPVSIQESVYRATVRLPQQQIIAYGELLPLQPEMILDAD
                     IHLDRRRLIEWVFDPLLSITGRI"
     gene            complement(7018..7188)
                     /locus_tag="PKZ49_02580"
     CDS             complement(7018..7188)
                     /locus_tag="PKZ49_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35476.1"
                     /translation="MNEFDFNESVSLDEIEVHTLTADELSLIAGGMMKMDEGVNVGKL
                     AAYKEMYGDMWF"
     gene            complement(7311..8348)
                     /locus_tag="PKZ49_02585"
     CDS             complement(7311..8348)
                     /locus_tag="PKZ49_02585"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HND35477.1"
                     /translation="MGPTGLSRILQIHPSRQCNLSCLHCYSSSSPHEKEHLTLKLLCD
                     AISDAAAEGFNYVSLSGGEPMLYKPLVELLEHAHQLGMRTAVTTNGMLLTEQKIAQLR
                     EVVDVLAISIDGVPSSHNRIRGSQIAFDTMRSRLPLLRAEGLTFGFIFTLTQYNLDEL
                     NWVREFAMGEGAKLLQIHPLEEVGNAATRLKGNSPDATESAYAWILAQQKIDSDIKIQ
                     VDLAFSEVIKQHPELVFAGDCANLETKPFGELISPLVIEADGTVSPIQHGFSRNFSVG
                     NLKAFPLSTLLEDWREYGLPAFHALCREVYEEIANTSSPLFLNWNSLIGKRAESALFN
                     PQDQIRKFMVV"
     gene            complement(8363..8581)
                     /locus_tag="PKZ49_02590"
     CDS             complement(8363..8581)
                     /locus_tag="PKZ49_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35478.1"
                     /translation="MSQEMRGSEKLKATCQTMASLTPEQIAQVAGGFSWSGQISPYWL
                     INGIPFDPHFIKFQPLQLNEFNINQQQF"
     gene            complement(8952..9197)
                     /locus_tag="PKZ49_02595"
     CDS             complement(8952..9197)
                     /locus_tag="PKZ49_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35479.1"
                     /translation="MSNEVNSREKMGGMTVLDIDALQAVSGGWCGTPYPGSWGIPVVP
                     HPEPDPWVNVFSSKLSLVSLNPQPLPPAELQGFTVGY"
     gene            9420..10574
                     /locus_tag="PKZ49_02600"
     CDS             9420..10574
                     /locus_tag="PKZ49_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HND35480.1"
                     /translation="MAFKGQITHLSQKGLGVVQNKQDGIAYFVSGTWPGDHGEFEIID
                     RTLPNKKYGYAKLVQLIQPSVERQQAPCPFLGTTETACAGCPWMIASYTSQLEQKRNR
                     FKYALQRTGFNLESLSIPPVHPSPVQYGYRNRFQLKTDGNQLGFVAEGSHRIAPIQDC
                     LVLNEACKTLLHKLHNQLPRSDWQPIKGANWNFIDLDDDLSFEQIRLNHKRPFKQGNC
                     AQNAWIKAWLSSKLAANPVPQKIIELFCGSGNFTEILANSGCRSIIAYEAELDAIQQL
                     RAKHLPNVDAQSIDLFKPLVWKIIKKVISDADTLVLDPPRAGLKNMKGFFEHFLALRT
                     IYYISCDPESFARDAWHFTKKGWKLAEIQLIDLFPHTPHVETLAILKKQQ"
     gene            10697..11743
                     /locus_tag="PKZ49_02605"
     CDS             10697..11743
                     /locus_tag="PKZ49_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HND35481.1"
                     /translation="MKTDTIQITPELLVLIAEIDEFKGAWRALGTLAPERLKALRHIA
                     TIESIGSSTRIEGSKLTNREAEQLLGSLEIKRFSSRDEQEIVGYAEVMETVFYVWADI
                     PITENHIKQLHRDLLRHSDKDERHRGEYKTLRNDAGAFDAAGNMIGIVFETATPFDTP
                     RRMQELVTWLNEARERKHLHSLLIVAVFIVTFLEIHPFQDGNGRLSRILTTLLLLQAG
                     YAYVPYSSLESAIENSKEGYYLALRQTQGTIRTETPNWQPWLVFFIRALQQQKSHLAL
                     KMEREKNTLAVLPALAVKILDYVRDHGRVTTRDMVRETGTSPNTLKATFTSLVEKGFL
                     VRRGGGRSTWYALP"
     gene            11867..13312
                     /locus_tag="PKZ49_02610"
     CDS             11867..13312
                     /locus_tag="PKZ49_02610"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HND35482.1"
                     /translation="MKAQKEELAIVLGQFNPWWRGESIPDLPDWHRASFGELMQWIMD
                     PPAARAIFLSGARQVGKTTLLLQAINHLLAQGIPASNLLYATFDHPIIKLAGADAVIQ
                     TWREREPKREGQEFIFLDEAQFINEWGTWIKHQVDFFKQRRIIFTGSAMPILQADQES
                     GVGRWHSIRLTTLSFYEYLQLKTITLPTLPTLRSLKELFTWSPNELRHISEQAQPYIG
                     HFHNYLVYGGFPQTALVKSVTQAQKLLREDIIDKAIKRDMTALFGVRRILDLEHTFLY
                     LCRHDGGLLDLTALSSNLKVTRPTAQHFIDLLEAAHLVYRLAPFGYGKEVLRGRYKIY
                     LADAAIAPAVMLKGQSIIDDPDALGIAAEASVLKHLFARYYQQNVRFTYWRGKKELEV
                     DLVAEIAGQIIPFEVKYRSQPVDARSLKGLIAFCHEKSIKRGYIITKSLDDLGLLPQK
                     EENAVPLFRIPATLLCFWMGEMELRQQQEID"
     gene            13430..14659
                     /locus_tag="PKZ49_02615"
     CDS             13430..14659
                     /locus_tag="PKZ49_02615"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HND35483.1"
                     /translation="MKDFFISYNSADEDDATWIAWQLEETGYTVIIQAWDFKKGNNFV
                     IEMNKASIAAKQTIAILSPNFLASQYTLPEWAAAFAQDPTGTQRKLIPVRVKPVEITS
                     GLLAAINYIDLVGIDRETAKQKLLQGVKGERLKPATEPTPSFHLQPNEKIKAPNNFAI
                     AEYKIGQLNRTTQYKHFKAHIPKECCLQQGKPFGFILAGPEQEWPVAIRFRLAYLLEK
                     VLIGDSKHMPNFVRLNTDLGLANEPVENDNQYLWDLLGDALNCQREQTALQEKLSQLP
                     ECHIFFREALQEEINAPNFLANLLSAWKNLQLHPSSKSHFLLFICEIDNPQQSWQEQI
                     ASALQQSNIEQALLPQLQSLNYHGDIKDWIKLNIDNDTLRDSISEALAEFAQQADIPL
                     IKLKKILKPLIERYPSH"
     gene            14670..15803
                     /locus_tag="PKZ49_02620"
     CDS             14670..15803
                     /locus_tag="PKZ49_02620"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HND35484.1"
                     /translation="MTTTSDGDYYTGKGEQKESSAWQNLPSFDPDELTHPKDYLAPAG
                     LAAAVNVALELGLPLLLTGEPGCGKSQLAYSLAWELGFPTQPLSFTVKSDTQSKDLFY
                     HFDTLGRFRAARIEDEEADARRYLRFEALGLAILHALGPDRIHALGLTSCLETLPPQP
                     QRSVVLIDEIDKAPREIPNDILNEIDRMSFDIPEIFQAGKARTVVSLSNHDNHGKQNL
                     LRPIVIITSNRERELPEAFLRRCVYYHLDIPPFRRQLTNGSNPDQGITLEQIVEKRLG
                     FEFDSRPDQPNNQKNLWNAAISLFDYLRNEIVLQKNPSTAELLNWIQLLKKRGGSPQL
                     TLSEASWRNLFVSSAKVTLFKHQEDQNRAQQLIEKWLELPVTA"
     gene            15814..19065
                     /locus_tag="PKZ49_02625"
     CDS             15814..19065
                     /locus_tag="PKZ49_02625"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HND35485.1"
                     /translation="MTTTIETAFHHLDQLADLLRAEGFACGPDVWLSVYRLLAQFKRK
                     NKLPHDTDQLRLLLGPLFCRNPEEQARFKAIYERWWTIHTQATSADYQKDGQSDAFTL
                     SAPNRLSAPGQAALIALRQKIKQVGRKWMFAIFAVIAVVGTLIYFSLKYPTPPVTTPP
                     QKQLTVPSKPDGDKQPSTQALPSTPIIDHIAPRSQPEPLRLTPNEKQWLTTLAYTVLA
                     LPWLIALLGLMRHYHAQRILKRQPATGDDLFNQLRFEKVLTPIWGGAQAEQALRNLRA
                     AQLIPSHRLDIEATVEATARQGDYFYPVYRNRRIAPEHLMLVRSMHRNDQKAALVEEL
                     EQRFRELGLHIETYRFRDDPRWLMRWKDDTPTKPQYIHLDQLYARFNGARLIIISETD
                     IIFHPLSGEPRLWLDALTPWRDKIWLHPQDAHAPHAALLAGHQFLLLPLLRASFAQWV
                     NYFTTAQRTEPVAVQSANPIKLPELIISEPDAWLTEKPPYGVDLTQLTQQLAQFLGPY
                     GLRLLRTIAAYPKPNWQLTQALDYLLYGHLNTVQQTMDPPERREQRLMRISRLPWLTH
                     AHFPYWLRVHLLSDLDDTERERISSAWQRLFTQLTNKESAGTLKLEIRTLSKQALLLR
                     LNELQASDTKQAINDPIFVQVLLGGQLDPLDFKLPSASAKELPHATRLLILRPALIVS
                     FLAAIATLILYVGWKHVDESGWYKLRHSLTVRENSTWPVQIRHAQTTQPLALALSEAL
                     RFKQFTVKLDDRVTPDSTTGDKQANANHIRYAPGGQAIADQIAVSLRWLTYGESIESE
                     EDSTLPASTIQVTLNRTYQPLTAFNDELHVPYVVPADEKSAPSTGLPFEEPQMVRIPP
                     GSFIVGSPEWEAGRRSDEGPQRKVTLDYPFEIGRYEVTFAQYDAFAKETQYPLPDDRG
                     WGRGDRPVINVNWHDAQAYVQWLAKKTGKKYRLPSEAEWEYVARAGTTTAYWWGEAIG
                     QSNAVCNGCGSQWDNQQTAPIGSFKANGFGVYDTAGNVLEWTQDCWHDDYTDAPTDGS
                     AWLEQNGGHCSRRVLRGGSWNFGPQDLRSALRYGVIPSGVNVNIGFRVARDLP"
     gene            19114..20217
                     /locus_tag="PKZ49_02630"
     CDS             19114..20217
                     /locus_tag="PKZ49_02630"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HND35486.1"
                     /translation="MNVYPYLIGFILTLLIASNLLAVANNDSAAEAKQIQSTQTQPSQ
                     QQPQQSNLESPLQPIIIEPLGKPDDAAQAVTKRLAATCQTDPQQAPTMVIIPPGQFLM
                     GSSPDTDADHQSDEAPQHWVTIPKPFALSRCEITVGQFRQFVQETRYQTTAETDGKGC
                     YSWDTAQKKAIQHSSLNWQNPGFSQDDHHPVVCISWQDTQHYLAWLSQRTEVRYRLPS
                     EAEWEYAARARTITPYFYQPDQQCTYANGAGQETKAIAASNWMLAQCTDSFVYTAPVA
                     SFIENPFGLFDMAGNVWEWTQDCWHDNYTEAPTNGSAWLDQNDGDCSRQVLRSGSWNF
                     NPQNLRSAIRFRLSPDDTFNINGNIGFRVARDF"
     gene            20540..>21095
                     /locus_tag="PKZ49_02635"
     CDS             20540..>21095
                     /locus_tag="PKZ49_02635"
                     /inference="COORDINATES: protein motif:HMM:NF025149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4172 domain-containing protein"
                     /protein_id="HND35487.1"
                     /translation="MNHYIHELPDWPDFYWDHKKLSGLLASVRHRQGRLIGKMEALGF
                     PSQAEATLQTLTLDVLKSSEIEGEILDRDQVRSSIARRLGLDIGALVPTDRHIDGIVD
                     MMLDATQNYAQPLSRERLFGWHAALFPTGYSGMHKIIIGQWRDDSNEPMQVVSGALGK
                     ERIHFEAPAANRLKKETDMFLTWFN"
BASE COUNT         5978 a         5367 c         4246 g         5504 t
ORIGIN      
        1 caacatgata agccccaatg tgctgggtaa ttcctcccgc ctctcctcct gcaacacgcg
       61 ttcttcgaat ataatccagc aaggatgttt tcccatgatc aacgtgaccc atcactgtca
      121 cgacaggggc acgcggctca gctatagcgt ctgactgaga agctttctct tcttccaaat
      181 aagattcagg attatcaggt gccgcatatt tcgcaatatg acctaattcc tcaaccacga
      241 tcattgccgt gtcttgatca agcatttgat tgatcgttac cataatgccc attttcatta
      301 aaacccggat tacttcagcc gctttaactg acattttttg agcaagcgca gcaactgaaa
      361 tcgtttcagg gaccattact tcatatttaa taggctctgt cggctcagta aaccgatgta
      421 actcagcgct ctcattggag cttgccttag catttttatt ggttttacga atacgccaac
      481 catcagcttg gtttaagtca tgttttttta tttcacgacg cttacccgtt tcttcactta
      541 gcagattctg ctgtttgatt tgctttttct ttttctccgg cttttcttca tgtttaaccg
      601 taatagtcga tttaggctgt tgcgccgtcg gttgagacgc cagctctgct gtgtttgtca
      661 ctttctccac aggtacttct tgcttaggtt cttcctttat aacctcacta acttttggct
      721 gtatcttctt ctcagctttt tttctgaatt caatttcttg gagttcaatc aactttgcat
      781 ggcgtctcgc ttcttcctca cgctgcgcta gctcatcagc actaataatc ggttttctta
      841 cagaagaggt ctccggtaca ctaacagaaa cttctatcgg agcaatcgct gctttggttt
      901 ctttatcttc agattgctca actgtttctt ctacaggctt tgtataaaca cgcgttttac
      961 gaactttaac ctcaatcgtc cgctgcttac ctgccacatc agattttttt atttcggttg
     1021 ttttcttacg ggttaatgca attttcttct ttgttgtttc tgccgcacca tgagacttac
     1081 gaagatactc gagcaactgc tttttatcgc cttctgtaat aatttcttca ccagattgct
     1141 tactaacacc agcagtttga agttgctcta gaagcgcatc aggtagcata cctaactcat
     1201 tggcaaattg ttcaaccgtt atttgagaca tcaaaaaccc ttcacttact ccaaatttat
     1261 aaattgcaga cttattcccg cattggttga ggctctcatt gacggaaatg ataacaacct
     1321 catacttatt caataaaagt tacacaaacc aaggctctcg agccttaata ataatctgat
     1381 tcgctcgctc aatatcgata ccagtcatct caaccaaatc atctgctgct aaatccgcta
     1441 gttcttcttg tgtattaata ccttttgcag caagttcccg aaccagttga atatccattc
     1501 cttctaaagc gagcaagtcc tcggaaacgc gttcaattct ctcttcagtc gcaattgcag
     1561 cggtaagcaa tgcattgcta gctcgatgac gtaattcagt aatcgtctct tcatcaagcg
     1621 cttcaatttc aagcatttct tcgagtggaa cataggcaat ttcttctaat gtagaaaacc
     1681 cttcttctac gaggatagcc gctacttctt catcaacatc caatttatcg atgaacagtt
     1741 tactgatgac tgaagtctct tcttcatttc gtttctgaga ttcctcctct gtcatgatat
     1801 tcaataccca acccgtcaac tcagaagcaa ggcgtacatt ttgaccacct cttccaatag
     1861 cttgcgctaa attttcatca ttaacaacga tatccatcgc atgcttatct tcatcaacaa
     1921 cgatactact gatttctgct ggagcgaggg cattaaccac ataagtcgca ggatcctctg
     1981 accatagaat gatatcaatc cgttcacctg tcaattccgt tataaccgac tggactctcg
     2041 agccacgcat tccgacacaa gtaccaatgg gatcaattcg tggatcgttt gatttcacag
     2101 caattttggc acgtgagccc ggatctctcg cagcagcttt gatttccagt aatttttcct
     2161 ctatctcagg cacttccagt tgaaatagct taatcaaaaa ttcaggagaa attcgagata
     2221 tgactaagct cggcccacgc gaagtccgat cgactctttg aagataaccg cgaactcgat
     2281 cccccaccct caaattttct ttaggaatca tttgatctcg aggcaaaatc gcttcaattc
     2341 tacccgattc tacaatggcg ttaccacgct ccatgcgctt gatcgtaccc gaaatcaaat
     2401 attctttacg atcaagaaaa tcatttagat tttgctcccg ttcagcgtca cgaatttttt
     2461 gaaaaatgac ctgctttgca gcttgtgcac caattcgatc aaactctaat gtttctatag
     2521 gctcttcaat aaaatcacct aatttgatct cagcattaag ctttaaagcc tcgctcaata
     2581 taacctgccg aaaaggattc tctaatgcat catccggtac aacctgctta ctctgaaagc
     2641 atcggtaatt ccctgtatct ctttctatcc taacctgtac ttcaatatct tcacggaaat
     2701 gttttttagt ggccgacgcc aatgccatct ccaaagcagt aaaaacaacc gctttactga
     2761 cattcttttc atgcgccaat gcatctacta atagtaaaat ctcgcggctc atagtcctcc
     2821 aaccaaatat ttattacgcc tatagttctg gaaccaaccg tgctttatct aaattattta
     2881 actcgatatg tagaattttc ccttctatct ccaaacctaa tttaccgtca ctcacttccc
     2941 gcagaatccc aacaaaattt ctctggccct gcaaagcaat atgcgattta accttaacta
     3001 atcttcctaa aaaacgcaca aaatcacgct ctttcctgag aggcctatcc aaacctggtg
     3061 aagaaacttc aagtcgccca taatcaattt gttctaccac cagcaaccga ctcaaatgat
     3121 tactgatcgc cacacaatca tcaatggaga tcccaccttc cttatctaca aatacacgca
     3181 ataacttgcc agatggtgaa aattccacat ccaccaattc atagcccata ttgttcagtg
     3241 ttgaatccaa taattcgtat aaagccatag cgcaaccaca aataaaaaat gggctaataa
     3301 gcccacccct gatatacgct ctattataac aacaatcaga tatcaacaaa atagaaattt
     3361 attttctagc aatgtttcca atatgttagc gcccattatc caatcaaaaa aacaacattt
     3421 gcattaatat taacctgagc aacctccaaa tctgctggta aatttccatt gattatttct
     3481 aacctaaagt tttcagtatt taatttgccg caatcacata ttgactttcc aactcattca
     3541 gctgcgattc agaacgataa tttgccattg ctggaacaat cgtatcacta acaatcctgc
     3601 cagcaaccaa ccgaatcact cgatctgcgc ttgcaatcgt ttcgggacgg tgtgcgacga
     3661 taattcgcgt taacttaagc gtgtgaatcg cttggttaac attctgctca caatgaatat
     3721 cgagatgact cgttgcttca tccaaaaaaa gaatggccgg ttgtcgatac aatgcacgtg
     3781 cgagcaatac ccgctgtttc tgtccaccag acaaaacagc ccccatatca cccactaacg
     3841 tattgaattg catcggcatt gccataatat catcatgaat ggcagccaga cgagcactag
     3901 ccataactcg ctcaaaatca ggggttgcat caaaaaagca aatattctcg gcaatcgagc
     3961 ccgcaaaaag ctgatcatcc tgcatcacac tggccacagc ctgacggtat cgcgccagac
     4021 tcatccggct caaaggcaca ccatcgattt cgatctgtcc gcctgtgggt tgtaacaaac
     4081 ctagcatgag tttaaccaag gtcgttttac cacagcccga tggcccaaca attgccactg
     4141 actcacctgc ttgaacgctg aactgaatat cttgcaaaac cagaggatcc gtttcactat
     4201 gccggaaagt tagatgttta acctcaattc gacctgttag gggggtagca gaaatcggat
     4261 gtagttcgtt aacttgttca atcggtgtca gcgccacgtc tcccactcgt tcggtatgaa
     4321 gacttaacat acgcagctct agtccttttt cgattaatgc tgaaattctt tgcacaaact
     4381 gagttttata cgcaataaac gcaaatagca ttcctatcga aaatccagtt cctgttgctg
     4441 tgtcgagtat caacatcgcg ccgatccata tcgtaataat attttccaag ccgaataaaa
     4501 gaccatttaa gccctggtat aaaatattga atcgctgagt acgaatgctc gcgttgaact
     4561 gatcgatcac caaattttgc caattcgcag cgcgtgatgc ctcataagca aacagcttaa
     4621 tactttgtat cccgcgtacg gtttccagaa aatggctttg ctgctttgcc ccccttacga
     4681 tatggtcttc tgtcgccaaa cgaagtggcc gatatagcac taaacgcagt aacccatata
     4741 atcctgctgc ggccattgca atcaacgcga gcttgacact atagagcagc atcataccta
     4801 acgtaatcaa caccatcaca ccatcgatta atgcttcgag aaaacccgtc gtcaaggttc
     4861 gctgaataac attcaacgag tcgaaacgag acacaatatc gccaatatga cgcttattga
     4921 accactgcat tggcaagcgg atcaaatgat ggaataagtt tgtcatcatt tgcaggttca
     4981 gcgttgtact taacaccatc aacacccaag atctcagcgc agtcacactc acttgtacca
     5041 cggctaacaa aagaaatccc acacccaaga tggtcacaag atcatgatca taggcgacca
     5101 acgcatggtc aacgacccac tgcatataaa aaggtgccaa aatggcaaaa acttggagga
     5161 tcaatgcgag aatcaagatt tggctaatcg caccagttaa accccgcaat cgaccaatca
     5221 aggaccgcaa cttgactcgc tgaatctcat tgcgcgtaac aaaatcacta gtaggggtta
     5281 attcaagtgc aatacccgta aaatgctttg agaattcagc caacgtcaat tcacactcac
     5341 ccgaggcagg atcatgcagc acgacccggt cacgttgcac gcgtgtcaag accacaaaat
     5401 gattaaaatt ccaatgtaaa atacaaggta gtttcaactc agctaactga tccagatcca
     5461 gacgtaaggg tcgtgaagcc agcttcagtt gctcagcaat ttgaatcaaa tccgcgagcg
     5521 tagagccgcg cagcgaggca gcatgctgtg cccgcaatcc gttcagattg atttgatgcc
     5581 catgaaaact cgcaatcatt gctagacagg ccaatccaca ctccgctgcc tcagtttgta
     5641 gaataaccgg tgttcggtta aataaagaga aatgcaacat gatcaaatac gtcctgtaat
     5701 cgacaataaa ggatcaaaca cccattcaat caaacgtcgt cggtccagat gaatatcagc
     5761 gtctaatatc atttctggtt gcagtggaag caactcaccg taggctatta tttgctgttg
     5821 tggtaggcgc accgtcgcac gataaactga ttcctggatg gatactggta aattagcctc
     5881 attgggttga atcacagcac gactaacctc gataatctcc cccaaatgat gtccaaagcg
     5941 ttgataagga aatgcctgat aacgcaaaga aaccatttgc cctggtttga tgaatccaac
     6001 tgaatgcgta ggaacgagca attgggcctg caactgcgca ccggcaggca aaatagacag
     6061 caggggcaaa ttaggttgag ccatttgccc catttcggct aaaagtgtag tcactatccc
     6121 ttcagtcggc gcagtcagca cgatactgcg acgcagatct gtttcggtaa gttgttgctc
     6181 caattcggaa atctgccgtt cgatcgctgc tcggttattc actttcttta gaccagaagc
     6241 tgacaactcg gatcgagccg tattcaattc acgattgagg ctagcaatag tccgtttgat
     6301 ctgaacaagc tgattacgct gatcaagcaa tgcttcttgt ttctcctgca gagcaacttc
     6361 cgacatgaat cgctctgcca ccaattgctg atagcgtacc accatacgct ctgcactggc
     6421 tacacggctc gattgcaatg ctaattgcgc ctgtgtttca cgaatttcat tctcaagtcc
     6481 acgaatgcgt gcattaatgc tggtaccggt tagtgcatcg atttcgctct gtttaccttg
     6541 ctcaaaacgc aagctcacct gccgctgttt taactcggtg agcatcgctg cttgagcctc
     6601 acgcgcgtta cttgttgttc gttcactcga aatcgtcaat aaaggatcgc cttgttttac
     6661 aacttgccct tcccttacat gcttctcctg tatggtacct aattgcggtg aataaatacg
     6721 tatcatacct gtggtgggcg ataaatagcc taccacatgg gtcttacgcg tatactcacc
     6781 ccaataagca aaagccagca acccaagggt aaaaataagc gccacccaga caaacaaacg
     6841 aatcgttaat gatcgcgcaa tcagtacttc accttgtcga gtatcttgct gatgggcgat
     6901 tgcctcagaa cgaaaaagcg gtcgtgccat gcacaaactc ctggaaatga aatagtcact
     6961 gcattcaaat aatggtgcaa tcgcgagtat attgcgattg caccgaacta ctattcatca
     7021 aaaccacata tcgccataca tttctttata tgcagcaagc ttgccgacat taaccccctc
     7081 atccattttc atcatcccgc ctgcgatcaa acttaattca tctgcggtca gcgtatggac
     7141 ttcaatctca tctaaagaaa ctgattcatt aaaatcaaat tcattcatga ttattctcct
     7201 aagcaattta atttaattaa cttcattcaa agttgactaa gctttctctt ctaagttaac
     7261 cctgtaagcg ttaacttact gcttctaagc aaaattgact gtgcagaaac tcacaccacc
     7321 ataaattttc taatttgatc ttgtggatta aaaagagcag attctgcccg tttacctatc
     7381 agactgttcc aattcaaaaa taaaggtgag gaagtattcg caatttcttc atacacttca
     7441 cgacacagtg catgaaaagc tggcaagcca tattctcgcc aatcttccaa taaggtactc
     7501 agcggaaatg cttttaaatt acccactgag aaattccgcg agaacccatg ctgaatcggg
     7561 ctgactgttc catccgcctc aatgaccagt ggtgaaatga gctcaccaaa cggctttgtc
     7621 tctagattag cgcaatcgcc tgcaaacacg agttctgggt gctgtttaat cacttcgcta
     7681 aaagcaagat caacttggat tttgatatcg ctgtcgattt tttgctgtgc gaggatccag
     7741 gcataagcgc tttcggtcgc atccggtgag ttccctttta atcgagtcgc tgcattgcct
     7801 acttcttcga ggggatgaat ttgcagtaat tttgcccctt cacccatcgc gaattcacga
     7861 acccaattaa gctcatccaa gttatattgc gttagcgtaa aaatgaaacc aaacgtcaaa
     7921 ccctctgcgc gtaataaagg taagcgggat ctcatcgtat caaacgcgat ctgagaacca
     7981 cggatccggt tatgagatga gggaacccca tcgatactga tggcaagcac atcaacaacc
     8041 tcacgcagtt gtgcaatttt ctgctcggtt aacagcatgc cattcgttgt aacggctgtg
     8101 cgcattccta attggtgagc atgctcaagc aactccacta acggcttgta aagcattggt
     8161 tcaccacccg aaaggctaac gtaattaaaa ccttccgctg cggcatcaga aatcgcatca
     8221 cataacaact taagcgttaa atgctccttc tcgtgcggag aggaagagga atagcaatgg
     8281 agacaactca aattacattg ccgagaaggg tgaatctgta aaatgcgaga taatccggtt
     8341 ggccccatcg ctagcctcct tcttagaatt gttgctgatt tatattgaac tcattcagct
     8401 gtaaaggctg aaacttaata aaatgaggat caaacggtat cccattgatc aaccagtatg
     8461 gagaaatctg tccagaccat gaaaatcccc ccgcaacttg ggcaatctgc tcgggcgtta
     8521 aacttgccat ggtttgacat gttgctttca atttttcaga accacgcatc tcttgtgaca
     8581 tgattgaatc tccttaaaaa aattaataaa ttgaataaat cgattgatga tccattgcgg
     8641 gctgaattcc aagatatctc cgccaagact acaaaccatc acgccatctt gattaccaac
     8701 tcgcattgca ttcaatctgg ttcaacaata attccaaatt cgaggcaaaa cagtgagaaa
     8761 aggcacaaaa gaacaaaact aagccattaa ttcgttttta aggaagggaa tgcgcctaac
     8821 ccaaagcgca aataacagga caaccctgcg ataccagaaa gaacatctgt ttaaacgaag
     8881 caaacattgg gagcaattga gtttatcgat aaacccgatt gctccctttg ctgattcaac
     8941 tcagcttatt gctaataacc aaccgtaaag ccttgcagtt cagcaggtgg taatggttgt
     9001 ggattcaagc ttacgagcga taatttgctg ctaaatacat taacccaagg atctggttca
     9061 gggtgaggaa caacaggaat tccccaagat ccaggataag gcgttccaca ccagcccccg
     9121 gaaaccgctt gtaatgcgtc aatatccaac accgtcattc cacccatttt ttcacgacta
     9181 ttcacttcat ttgacatgat cattctccta aaaaattaaa tcgattaagt gattatctgc
     9241 aaaataacaa tccactttgc actcaaccca gtcctaccat aactttcatt cacaaaagca
     9301 tcagtgtgta aaatcacgct ggcgaagctt gatcaggtct aaaaaccgac ttgaagcatt
     9361 attgagctta tgagaccatg cgcctcaaaa tcgcatcgac aaagaggaac gagtagcaca
     9421 tggcatttaa gggtcaaatc acgcatctct cacaaaaagg cctgggagtc gttcaaaaca
     9481 aacaagatgg cattgcttat tttgtttcag gcacctggcc cggggatcac ggtgaattcg
     9541 aaattatcga ccgcactctc ccaaataaga aatatggtta cgcaaaactc gttcaactga
     9601 ttcagccttc tgtagaacgc cagcaagccc cttgcccatt tctgggaaca acagaaaccg
     9661 cctgcgcagg atgtccatgg atgattgcat cttatacaag ccaactcgaa caaaagcgga
     9721 atcgctttaa atacgccctg caacgcactg gattcaacct cgaatcgcta tcgattcccc
     9781 ccgttcatcc ctcacccgtg cagtatggct accgtaatcg cttccaactc aaaacggatg
     9841 gtaatcaact tggcttcgtt gcagaaggct cccaccggat tgcaccgatt caggattgcc
     9901 tcgttctcaa tgaagcttgc aaaacgttat tgcataaact gcacaaccaa ttacccagaa
     9961 gcgattggca gccgattaaa ggcgctaatt ggaactttat tgacttagac gatgatctat
    10021 cctttgaaca gatccgcctc aaccacaagc gccctttcaa acaaggtaat tgcgcacaaa
    10081 acgcctggat caaagcttgg ctcagcagta aattagcagc aaaccctgtt ccgcaaaaaa
    10141 tcatcgaact cttttgtggc tccggtaatt tcactgaaat ccttgcgaat tcaggctgcc
    10201 gttcgatcat tgcctatgaa gcggaattgg acgcgattca gcaacttaga gccaaacatc
    10261 tacccaatgt tgacgcacag tcgatcgatc tattcaagcc cctcgtttgg aaaattatca
    10321 aaaaagtaat cagcgatgca gatactctcg tgctcgatcc tccccgcgct gggctcaaaa
    10381 atatgaaagg cttttttgaa cactttctcg ctttacgaac gatctactac atctcgtgcg
    10441 acccagaaag ctttgcacgc gacgcctggc atttcaccaa gaaaggctgg aaactcgctg
    10501 aaattcaact gattgatctg tttccgcaca cccctcatgt agaaacactc gctatattga
    10561 aaaaacagca gtgatttttt acaaaaaaac tcagttatag acattccgca gcaagctgta
    10621 tcgttatatt ttcaggtata aatcggtatt tatttcggta gacaatccgg tataatccgg
    10681 catatattcc ggtattatga agaccgatac cattcagatc acgccagaac tcttggttct
    10741 gattgctgag attgatgagt tcaaaggtgc atggcgcgcc cttggcaccc ttgcaccgga
    10801 acgactaaaa gcattacgtc acattgccac cattgagagc attggttctt ccacccgcat
    10861 cgaaggtagt aagttgacca accgcgaagc agaacaatta ttgggcagtt tggaaatcaa
    10921 acgcttcagc agtcgcgatg aacaagaaat cgtcggctat gctgaagtga tggagaccgt
    10981 cttctacgtg tgggccgata ttccaattac cgagaaccat atcaaacagc ttcaccgtga
    11041 tcttttgcgc cacagcgaca aagacgagcg acaccgtggt gaatacaaaa cgttgcgcaa
    11101 tgacgcggga gccttcgacg ctgctggcaa catgatcggt atcgtcttcg aaacagccac
    11161 accattcgac acgccaagga gaatgcagga actcgtcacc tggctgaatg aagcccgcga
    11221 acggaagcac cttcactcgc tgctgatcgt tgctgtcttc atcgtcacct tcctggaaat
    11281 ccatcccttc caagatggca atggccgttt gagtcgcatc ctaaccacgc tactcctgtt
    11341 gcaggctggt tatgcctatg taccgtattc gtcacttgaa agcgcgatcg aaaacagtaa
    11401 ggaaggatat tacctcgcct tgcgccaaac ccaaggtacc atccgcacag aaacccccaa
    11461 ttggcagccc tggcttgttt ttttcatacg tgctttgcaa cagcaaaaat cccacctagc
    11521 tttgaaaatg gagcgtgaga aaaatacact tgctgtttta cccgcgctcg ctgttaaaat
    11581 cttagactat gtccgcgacc atggtcgcgt taccacccgt gatatggtgc gcgaaacagg
    11641 aacaagtccc aatacgctca aagcaacctt cacatcgtta gtcgaaaagg gatttctagt
    11701 acgtcgcggt ggtggtcgct caacttggta tgccctgccc tgatttagaa tttccccacg
    11761 cgcgctattg aaaaaatagc agtaattttt cacaaaaaat cattaaaaat gatttatttg
    11821 tatagtatag tttaactata tgatgaattt aagctaaaaa tacgtaatga aagcacaaaa
    11881 agaagagctt gcgatcgttc tagggcaatt caatccttgg tggcgtggtg aatcgattcc
    11941 agatttacca gactggcacc gcgcttcatt cggtgaatta atgcaatgga ttatggatcc
    12001 gcctgctgcg cgtgctatct ttttatcggg agcacgtcag gtaggtaaaa ccacgctact
    12061 gctccaagca attaatcacc tcctagcgca aggtattcca gctagcaacc ttttatacgc
    12121 gacctttgat caccccatta tcaaattagc aggcgctgac gcagttattc aaacctggcg
    12181 agagcgtgaa cccaaacgag aagggcagga attcatcttt ttggatgaag cgcaatttat
    12241 taatgaatgg gggacttgga tcaagcatca agtcgatttc tttaaacaac ggcgcatcat
    12301 tttcactgga tctgccatgc caatcttaca agccgatcaa gaatctggcg ttggtcgctg
    12361 gcacagcatc cgcctaacaa cgctatcctt ctatgaatac ttacaactca aaaccataac
    12421 gcttccaaca ctaccaacct tacgctcgct caaggaacta tttacttggt cccccaacga
    12481 gttgcgccat atcagtgagc aagcccaacc gtatatcgga cattttcaca attatctggt
    12541 ttatggcggt tttcctcaaa cagcgctagt gaaaagcgtc acacaagcgc aaaaattact
    12601 acgcgaagac atcatcgaca aagcgatcaa acgcgatatg accgcacttt ttggtgtaag
    12661 gcgcattctt gatcttgaac atactttctt atatctatgc cgtcacgatg gtggcttact
    12721 cgatctaacc gcattaagca gcaatcttaa agtgacacgc ccaacggctc agcactttat
    12781 cgacttactt gaagcggctc atctcgttta ccgtctggcc cctttcggct atggcaaaga
    12841 agtattgcgc ggccgctaca aaatatatct cgctgatgcg gcgattgccc ctgcagtgat
    12901 gttgaaagga caatcgatca tcgacgaccc tgatgccctg ggtatcgctg ccgaggcatc
    12961 agttttgaaa catctttttg cgcgctatta ccagcaaaat gtacgcttca cttattggcg
    13021 tggaaagaaa gaattggaag tcgatttagt tgccgaaatt gccggtcaga tcatcccatt
    13081 tgaagttaaa tatcggtcac aacccgttga tgcacgcagc ctcaagggac tcattgcgtt
    13141 ttgccatgag aaaagtatca agcgcggcta tatcattacc aaatcccttg atgatttagg
    13201 tctgctcccg caaaaagaag aaaatgcagt gccgctattt cgcatccctg ctaccctcct
    13261 ctgcttctgg atgggagaaa tggagttacg tcagcaacaa gaaatcgatt aaagtctctt
    13321 cgctcactat ttttaagtca atttctcaaa catcgcggga tatgacttga atccgcccac
    13381 agttatgata gattcattat tatttaccta ataataaaaa gaatcagtca tgaaagattt
    13441 cttcatcagc tataacagtg cagacgaaga tgacgcaacc tggattgcct ggcagcttga
    13501 agaaactggt tatacagtta ttattcaggc gtgggatttt aagaagggga ataattttgt
    13561 tatcgagatg aataaagcaa gcatagccgc caagcagact atcgcaattc tctctcctaa
    13621 ttttctagcg tctcaatata ctctcccgga atgggcagca gcatttgcac aagatccaac
    13681 tggtacgcag cgaaaattaa ttccagtccg agtaaaacca gttgaaatca ccagtggttt
    13741 gttagccgca atcaattata ttgatttagt cggtatagac cgcgaaaccg ccaaacaaaa
    13801 attattgcaa ggtgttaaag gagaaaggtt aaaaccagcc acggaaccga ccccaagctt
    13861 tcacctacaa ccgaacgaaa aaataaaagc tccaaataat tttgctattg cagaatataa
    13921 aattggccaa ttaaatcgaa cgactcaata caagcatttt aaggcgcata tccccaaaga
    13981 atgctgtctt caacagggaa aacccttcgg ttttatatta gctggacccg agcaagaatg
    14041 gcctgtcgcg attcgatttc gcttagctta cttactcgaa aaagttttaa tcggtgactc
    14101 caaacatatg cccaacttcg tgagattaaa tactgacttg ggtttagcca atgaaccagt
    14161 cgaaaacgat aatcaatatt tatgggattt attgggtgat gcgctcaatt gccaacgaga
    14221 gcaaaccgca cttcaggaaa aattaagtca actgcctgaa tgccacattt tctttcgcga
    14281 agcgcttcaa gaagaaatta acgcacctaa ttttctagca aacctcctaa gcgcttggaa
    14341 aaacctgcaa ttgcacccca gctccaaaag tcattttcta ttatttatct gcgaaattga
    14401 caacccccag caatcctggc aagaacaaat agcatctgcg ctgcagcagt ctaatataga
    14461 acaagcgctg ttacctcagc ttcaatccct caattatcac ggtgatatta aagattggat
    14521 taagcttaat atcgataatg atacgttacg cgactccatt agcgaagcgc tagcagaatt
    14581 tgcgcagcaa gcggatattc ccttgattaa actaaaaaaa attctaaaac ctttgataga
    14641 acgttaccca tctcattaat ggagaaacca tgacaaccac ttccgatgga gattattaca
    14701 ccggtaaagg tgagcaaaaa gaatcatccg catggcagaa cttacccagc tttgatcccg
    14761 atgaactcac gcatcctaaa gattacttgg cacctgctgg acttgccgca gcagtcaatg
    14821 tcgcattaga actcggttta cccttgctat taacaggcga accggggtgt ggcaaatctc
    14881 aactcgctta tagcctcgca tgggaattag ggtttccaac acagccctta agttttaccg
    14941 ttaaatccga cacccaaagc aaagatttgt tttatcactt cgatacctta gggcgtttcc
    15001 gcgctgcaag aatcgaagac gaagaagccg atgcacgccg ttatctccgt tttgaagcgc
    15061 ttggactcgc catcttacat gcgttaggcc cagatcgcat tcacgcatta ggattgactt
    15121 cttgcctgga aacattgccg ccgcaacccc agcgttcagt cgtgctcatc gatgaaatcg
    15181 ataaagcacc ccgtgaaatt cccaacgata tcctcaacga aattgaccgc atgagcttcg
    15241 atattcctga aatttttcag gcaggaaaag cacgcaccgt cgtatcgctc agtaatcatg
    15301 acaaccacgg aaagcaaaac ttattgcgcc ccatcgtcat cattaccagt aatcgcgaac
    15361 gtgaattacc ggaagcattt ctccgtcgct gtgtttatta tcatctggat ataccgcctt
    15421 tccggaggca gctaacgaat ggtagtaacc cagatcaggg catcaccttg gaacaaattg
    15481 ttgaaaaacg cttagggttt gagttcgatt caagacccga tcaacccaat aaccagaaaa
    15541 atctctggaa tgccgccatt tcgctttttg attatctacg caatgaaatc gtgctacaaa
    15601 agaatccctc aaccgcagaa ctgttaaatt ggattcagct cttgaagaaa cgcggtggct
    15661 ctccgcaact aacgcttagc gaagcatctt ggcgaaatct gtttgtcagc agcgccaaag
    15721 taacgctgtt taaacatcaa gaagatcaaa acagagcgca gcaactaatt gaaaagtggc
    15781 tagaactccc agtgaccgct tgattcaatc gctgtgacaa cgaccattga aaccgctttc
    15841 caccacctag atcagcttgc agatctctta cgtgcggaag gattcgcttg tggacccgat
    15901 gtttggcttt ccgtttatcg actgctagcg caattcaaac gaaaaaataa actaccgcac
    15961 gataccgatc agttaaggtt attattaggc cctctctttt gtcgcaaccc tgaagaacaa
    16021 gcgcgtttca aagcgattta tgaacgttgg tggaccatac acacccaagc aacgtctgcg
    16081 gattatcaaa aggatgggca aagcgatgcc tttacccttt ctgcccccaa tcgcttaagc
    16141 gctccagggc aagcggcgct catcgcattg cgccagaaaa tcaagcaagt tggccgtaaa
    16201 tggatgttcg cgatctttgc cgtgatcgcg gtcgttggca cattgattta tttttccctg
    16261 aaatatccga caccaccggt aaccacgcca ccccaaaagc agcttaccgt tccatctaaa
    16321 ccagatggcg ataagcaacc gtcaacccaa gctttacctt ccacaccgat catcgatcac
    16381 atcgcgcctc gatcacaacc agaaccatta cgcttgacgc ccaatgaaaa gcaatggtta
    16441 accacgctgg cttacaccgt cttagcctta ccttggctta tcgcgctctt aggattaatg
    16501 cggcactacc acgcgcaacg gatactcaag cgtcaaccag ccactggaga cgatttattt
    16561 aatcaactgc gctttgaaaa ggtgttaacc cccatctggg gaggcgctca agcagaacaa
    16621 gcgctacgta atttacgcgc agctcaactc atcccgtcgc atcgtctcga tatcgaggca
    16681 acggttgaag caaccgcacg ccaaggtgat tacttctatc ccgtttaccg taaccgccgc
    16741 attgcgcccg aacatctcat gttggttcgc agtatgcatc gtaacgatca aaaagccgca
    16801 ctcgtcgaag agttagaaca gcgctttcgt gaattagggc tacacatcga gacctatcgt
    16861 ttccgtgacg atccccgttg gttaatgcga tggaaagatg acacaccgac taagccacag
    16921 tatattcatc tcgatcaact gtatgcgcgt tttaatggcg ctcgtctgat catcatcagt
    16981 gaaactgaca ttatttttca cccgttgagt ggagaaccga gactctggct cgatgcctta
    17041 accccttggc gagataaaat ctggttacat ccacaggatg cccatgcacc tcatgccgcc
    17101 ctattagcgg ggcatcaatt tttactactc ccgctcctgc gcgcaagctt tgcgcaatgg
    17161 gtcaattatt ttaccactgc ccaacgcaca gaaccggtcg cagtacaatc cgcaaatccc
    17221 atcaaattac ccgaattaat catcagcgaa ccggatgcct ggctaacaga aaaaccgcct
    17281 tatggcgtcg atcttaccca actcacccag cagctagccc aatttttggg gccgtatgga
    17341 ttgcgtttac tcagaaccat tgccgcatac cccaaaccca attggcaatt aacccaagcg
    17401 ctcgattatc tactatatgg tcatttaaat accgtgcagc aaacgatgga cccccctgag
    17461 cggcgtgagc aacgcttaat gcggatcagc cgtttaccct ggctaaccca tgcccatttc
    17521 ccttattggc tgcgggttca tttgttgtcc gatttagatg atactgaacg cgagcgcatc
    17581 tcgtcggcct ggcaacgctt atttacgcaa ttgacgaata aagaaagcgc tggcaccttg
    17641 aaacttgaaa tccgtacctt atctaaacaa gcgctcctgc ttcgcctcaa tgaattgcaa
    17701 gccagcgata ccaagcaagc cataaacgat ccaatttttg tgcaggtgtt actgggcggt
    17761 caattagatc cattagactt caaattaccc agcgcttcag caaaagagct accgcacgca
    17821 actcgtttat tgattctccg ccctgcgctc atcgtcagct tccttgcagc gattgccaca
    17881 ctaatccttt acgttgggtg gaaacacgtc gatgaatcgg gctggtataa actgcgacat
    17941 agcttgaccg tgcgagaaaa tagcacttgg ccggtgcaaa ttcgccatgc ccaaacgact
    18001 caaccgctag cgctggcgct cagtgaggcg ctacggttca agcaattcac ggtcaaactg
    18061 gatgatcgtg ttactcctga ttcgacaacg ggtgacaagc aagctaacgc taatcacatc
    18121 cgctacgcac cgggtggtca ggccattgcg gatcaaatcg ccgtgagttt acgctggctc
    18181 acttatggag aatcgattga gtccgaggaa gacagcacgt taccggcctc aaccattcaa
    18241 gtgacgttaa accgcaccta tcaaccgctc actgcgttta acgatgaatt gcacgtgcct
    18301 tatgttgtgc cagcagatga gaaatccgct ccgtcaaccg gtctaccctt tgaagaaccg
    18361 caaatggtgc ggattccacc gggaagcttt atcgtgggat cacctgaatg ggaagcgggt
    18421 cgacgttccg atgaagggcc acagcgcaag gttacccttg attacccttt cgaaatcggt
    18481 cgctatgaag tcacgtttgc gcaatacgat gcctttgcta aagaaacaca atacccactg
    18541 cccgatgatc gtggttgggg acgtggcgat cgacccgtga tcaacgtgaa ttggcatgat
    18601 gcgcaggcct atgtgcaatg gttagcgaaa aaaaccggta aaaaatatcg tttaccgagc
    18661 gaggcagaat gggaatatgt ggcacgcgct ggcacaacca cggcttattg gtggggtgag
    18721 gccattgggc aatctaacgc agtttgtaat ggttgcggca gtcagtggga taaccaacaa
    18781 accgcaccga tcggttcctt caaagccaat ggatttgggg tatacgatac cgccggaaat
    18841 gtattggaat ggacgcagga ttgctggcat gatgattata ctgatgcacc tactgatggt
    18901 tctgcttggt tggaacagaa tggtggccat tgctcgcgcc gggtgttgcg cggcgggtcc
    18961 tggaacttcg gcccacaaga cctgcgttca gctctccgct acggggtcat cccgagtgga
    19021 gtcaacgtca acataggctt ccgtgtcgcc agggatttac catgagcaag cgtttaattg
    19081 ctgtgcatag taaccgacta aaagcataat gctatgaatg tctacccata cttaatcgga
    19141 ttcatactca cgctgcttat cgctagcaac ttactagccg tcgctaacaa cgatagtgca
    19201 gccgaagcga aacaaattca gtctacccaa acccagccat cgcaacaaca accgcaacaa
    19261 tccaaccttg aatctccgtt gcaaccgatc atcattgaac cactcggcaa accggatgac
    19321 gcagcgcaag cagtgacaaa gcgtttggca gctacttgcc aaaccgatcc gcaacaggcc
    19381 cccacgatgg tcatcattcc ccctgggcag tttttgatgg gatccagccc ggatacggat
    19441 gccgatcacc aatccgatga agcgccgcaa cattgggtca cgattcccaa accgtttgcg
    19501 cttagccgtt gcgagattac ggtggggcaa tttcggcagt tcgtgcagga aacccgctat
    19561 caaaccacgg ctgaaaccga tggcaaaggt tgttatagct gggatacagc acaaaaaaaa
    19621 gccatacagc actctagctt aaattggcaa aatccaggat tttcgcagga tgatcatcat
    19681 ccggtggtgt gtatctcctg gcaggatacc cagcattatc tcgcttggtt atcgcagcgt
    19741 accgaggtgc gttatcggct gccgagcgaa gccgaatggg aatatgccgc acgtgcgcgc
    19801 accatcactc cttattttta tcaaccagat cagcagtgca cctacgccaa tggggccggt
    19861 caagaaacca aagcaattgc agcttcaaac tggatgttag cgcaatgcac tgactccttc
    19921 gtttataccg cgccggtagc cagttttatc gagaatccat tcgggttatt cgatatggcc
    19981 ggtaatgtgt gggaatggac ccaggattgc tggcatgata actataccga ggcacctact
    20041 aatggttctg cctggctgga tcaaaatgat ggcgattgct cgcgccaggt gttgcgcagc
    20101 gggtcctgga acttcaatcc acagaacctg cgttcagcta tccgcttcag gcttagccca
    20161 gatgatacct tcaacatcaa cggcaacatc ggctttcgcg tcgccaggga tttttgattt
    20221 ttgtgctttg gggtttgccc ctttgtgccg ttggggtgca ggggcgtagc ccctgcgcga
    20281 tttttttatt tcaatataat tcggggttga tttttaacta agcgcgtgat gcttagtcat
    20341 cttttgcaca tgtgccaaaa ataaaaatcg cgtgattcaa attctactcg tctgaagtct
    20401 atcctcgcaa tcgctaaatt cataccgaaa acatcagtcg atgcggcgaa tagccatccc
    20461 aataaccgca tcttatgcgg atattgaagt tgcaacatgc ggagaaaagg gtgcataatc
    20521 ttcgcatgag gtgcgtagaa tgaatcacta tatccatgaa ttacctgact ggcctgattt
    20581 ctactgggat cataaaaaat tatctgggct gttggcgtct gtgcgtcacc ggcagggacg
    20641 tttgattggc aaaatggaag cgttgggatt tccctcacaa gcggaagcca cactccagac
    20701 cctaacactt gatgtgctga aatcttccga aattgaaggc gaaattctgg atcgtgacca
    20761 agttcgctca tcgatcgccc ggcgactcgg attagatatt ggcgcgctag tgcccactga
    20821 ccgtcatatt gatggcattg tcgacatgat gctggatgca acgcaaaatt acgcgcaacc
    20881 cctcagtagg gaacgcttgt ttggctggca tgctgccttg tttccgactg gttatagcgg
    20941 catgcacaaa atcatcatcg gacaatggcg cgatgattca aacgaaccga tgcaggtggt
    21001 atccggcgcg cttggaaaag aacggataca ttttgaagcg cctgccgcca accgccttaa
    21061 aaaagaaacc gatatgttcc taacgtggtt taaca
//
LOCUS       DAOQKQ010000023        21040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_1090_length_21040_cov_6.254753, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000023 DAOQKQ010000000
VERSION     DAOQKQ010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 21040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21040
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1090_length_21040_cov_6.254753"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(665..740)
                     /locus_tag="PKZ49_02640"
     tRNA            complement(665..740)
                     /locus_tag="PKZ49_02640"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(705..707),aa:Lys,seq:ttt)
     gene            complement(835..3234)
                     /gene="gyrB"
                     /locus_tag="PKZ49_02645"
     CDS             complement(835..3234)
                     /gene="gyrB"
                     /locus_tag="PKZ49_02645"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HND35488.1"
                     /translation="MTNKDDREYDSDSIKILKGLDAVRKRPGMYIGDTSDGTGLHHMV
                     FEVVDNAIDEALAGHCDEIIVIIHPDNSVSVHDNGRGIPTGIKQDDELKRSAAEIVMT
                     ELHAGGKFDDNSYKVSGGLHGVGVSVVNALSEWLRLTIRRDKKIHQMEFRKGVPVAPL
                     AVTGESEKHGTEVHFLASKEIFGDVNYHYEIFAKRLRELSFLNNGIKIKLIDQRHDKE
                     ETFAFSGGIRNFVEYINRTKTVLHPAIFYANGSKDNIAVEVAIQWNDSYVEQVLCFTN
                     NIPQKDGGTHLTGLRAAMTRTLNSYIEKNELAKKAKVDTTGDDMREGLTCVLSIKLFE
                     PKFSSQTKEKLVSSEVRPVVEDIVAQKLTEFLLEHPSDTKIICNKIIDAARAREAARK
                     ARELTRRKGVLDNLGLPGKLADCQEKDPANCELYLVEGDSAGGSAKQGRDRKFQAIMP
                     LKGKILNVEKSRFDKLISSQEIISLITALGTGIGKDEYNPDKLRYHRIIIMTDADVDG
                     SHIRTLLLTFFYRQMPELIERGHIYIAQPPLYKIKHGKQERYVKDDHELNQYVLGLAL
                     EGAELQISESNPPIMGEALTSIAREYLLAEAVIDRMSRLIDRSVMYALLKEPDIDLSN
                     QDNAQQSAAKLAAIVQTVQINPEFDDVFEQYRLKLIRTHHGNIQTSYLDQDFVKSNDF
                     EQIKKAAQVLQGLITNGAVVKRGNDEHTITEFKEALDWLLAEAKKGINIQRYKGLGEM
                     NPAQLWETTMDPNNRRLLRAQIEDSILTDEIFTTLMGDVVEPRREFIEKNALQVTNLD
                     V"
     gene            complement(3275..4396)
                     /gene="dnaN"
                     /locus_tag="PKZ49_02650"
     CDS             complement(3275..4396)
                     /gene="dnaN"
                     /locus_tag="PKZ49_02650"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HND35489.1"
                     /translation="MNLINTKRDTLLKPLQIVSGIVEQKRTLPILSNVLIRKEQEKIF
                     FVTTDLEVEIEVHSIDNENLSKENFSITVSARKFLDILRAFSADTSVVLNKTDNRLQV
                     NISKSRFNLQILPMEDFPRMAIEEKPEVSLTLSQKDLKNCLYHVQYAMAQQDIRYYLN
                     GLLLLTDGQQLKAVATDGHRLGYISMQFEAELIKKETILPRKTVHELVKLLEDSDKTV
                     TIDFYSKKISFSFSGIILISKIIDGKFPDFDRVIPKNNTKQFIIKRLDFLQALQRAAI
                     LSNTSDKFRGVKLIIANNRLSILCKNSEQEEAQEDLDIDYNSQEIIDISFNITYLLDV
                     LNNLDSETIQCSFENANSGTLITIPGDEAFKYVVMPMRL"
     gene            complement(4609..5997)
                     /gene="dnaA"
                     /locus_tag="PKZ49_02655"
     CDS             complement(4609..5997)
                     /gene="dnaA"
                     /locus_tag="PKZ49_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HND35490.1"
                     /translation="MQEFEAFWYTCLKHFEKELNAQQFNTWIKPLQLDISEGCGDQPI
                     LIAPNRFVLQWVKDNFIPHIEAMAQQHFSKRIQFQLVLGEITTKKATPKVNPIIDTSR
                     EDQAVSTVSVADPTPSKKASNKHYLNPNFTFDNFVTGKANQLARAGAIQVAERPGTAY
                     NPLFIYGGVGLGKTHLIQAIGNFILERNHEAKIRYVHAEKYVSDVVSAYQHKSFDKFK
                     KYYHSLDVLLVDDVQFFGGKNRTQEEFFYAFNSLIEASKQVIITCDCYPKEISGLEER
                     LVSRFGWGLTVAIEPPELEMRVAILIKKAALEKISLEENIAFFIAKHIRSNVRELEGA
                     LKRVLAYSRFTGQDLSLELVKEALKDLLAVQNRQISIENIQKTVADYYKIKVAEMYSK
                     KRTRTIARPRQIAMAIAKELTQLSLPDIGEAFGGRDHTTVLHAHRKINELRASDPLIT
                     RDYNALIHILRG"
     gene            6263..7483
                     /locus_tag="PKZ49_02660"
     CDS             6263..7483
                     /locus_tag="PKZ49_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HND35491.1"
                     /translation="MTLKPAVLAHHLSKCYQLYTQPKDRLKQLIWGRRRQYYRELWAL
                     REINLAIMPGEVIGIIGKNGSGKSTLLQLICGTLTPTTGEVEINGRLAALLELGAGFN
                     PEFTGRENIWMNAAIIGLTQQEIADRIEQIIDFSEIRPFVDQPVKTYSSGMYVRLAFS
                     IAISVDPDILIIDEALSVGDGAFARKSFNRIMQLRDSGKTILFCSHSLFQIESLCNRA
                     FWINQGELIAEGDVKQVVTAYQQFLDLGVLSSDNNDIAATIQQNDYLANAKGYARLET
                     VVVEIDGAPCAVAVAESGQSAIQVDVKFSTDPSLPCPTVAVTLHSMDGRVITSAASWE
                     DSIIPRLTPEGIGEIKLLFEQLPLLKGEYLVSVYLLCEQGIHLYDSVYGATKLQVQQQ
                     NRLQGFFMIPHQWK"
     gene            7563..9365
                     /locus_tag="PKZ49_02665"
     CDS             7563..9365
                     /locus_tag="PKZ49_02665"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HND35492.1"
                     /translation="MNGFSSKQEISDGSITINSPSLFKNFDFPLNVYAHTLFLNEGRV
                     DYLHYGLFESEQINLSQAQQYSTNLILDRLPLPPSRILEVGAGLGTTCHLLTQRSYQV
                     HSITPDRQQIAEIHRRFATGLNVTQQRYEDLELITESYDVILFQESAQYIEPLVIFNK
                     AQDLLPEEGHIFIIDEFALQQSETAIGRLHRLKAMLQLSDRLGFELVEHLDLSSKATP
                     TLKYLLHAIGKHRQQLLDDLDLESSRLDQLVQSNQDYLNKYVSGQFGYALLHFRKKRQ
                     LPWRLSRLNTNQMPHMLALFEKIFKHKMTSEMWQWKYGSEQCRAMGVWYDNELIAHYG
                     GMGRSILYFGQPQMAVQIGDVMVDRVNHPGLVKKGPFFQMAATFLEHYIGYGKPYLLG
                     FGFPNDRAMKVAKRHGLYAETGRMVEIAWTPYSRRPLWLSQLKIIDQHNINDNWVVDA
                     INQCWHGMASDLNAAIIGIRDWAYLRDRYLNHPQHHYRIVLVLSRLSRKARGILVLQS
                     NSNGCEIMDMVAPLDQISLLITHTRRIARINGDQRVFCQITHNFSDRFISASGEGMLS
                     ELPIRIPANAWSDGPSPQLLMNHWWLMSGDMDFR"
     gene            9404..12337
                     /locus_tag="PKZ49_02670"
     CDS             9404..12337
                     /locus_tag="PKZ49_02670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HND35493.1"
                     /translation="MENLLIPYQATTIIPCKKVLVFAPHPDDEVFGCGGAIMRHLAQG
                     VAVEVIIVSDGAYGAKSEESSRQYVLQRQEESNLAAKILGYGLPSFWSYPDRKVGYGE
                     KLIQDILAAINQSQADLIYAPSVFEVHPDHRSIGMAAIEAVRRIGKSLKLALYEVGMP
                     MRPNCLLDISEFVERKMAAMQCFSSQNQKQRYDLDIAALNRYRTYTLPAEIQAAEAYL
                     IISAEELVKDPLKLYQSEYARQRELGVAIDRGDIPLVSVIIRSMDRPTLTRALDSLAL
                     QTYPNIEVVLVNAKGGMHEQSSDWCGNFPLRLINQDGEPLLRSKAANIGLDACQGSYI
                     SFLDDDDAIDPDHIANLIEGIQSVSTCSIAYTGVRGKDEQDENKPFVEFKTAEVNFAK
                     LLLGNVIPIHAILFPATLLKQGAVFDEKLALYEDWDFWLQLSRYTPFNFINRTSATYF
                     TGGGSEVSPLHNDPVRVKQATVNLYQKWLKLLTASEFAAVADLHHRTNNQFQAEMGGL
                     RSEISRLQDLQQQTRFQLEQKQQELDILYASRSWRLTLPLRCCLERVRALKSVFKRCY
                     YTLPGAYRIYRFVREFSQQRRRQSSSQIDQSGGPWPIGPAGQILLIEHSVPRPDQDAG
                     SMMIYNFIKVFIEMGYGVTFYPVDLAYDPVYTPRLIELKVRCLNKPQVNALEHHLAAI
                     DRSYDFVLSCRPVYTEALIPIIKKYCTTAHFIYETHDLHFIRLQRQAEVEHNREMLAH
                     SSFLKAMELNIAAEADCTIVVSHKEKEILLQENPNLYIEVIPVVSEVFGRKSDFTQRN
                     DLVFIGNYEHRPNVDAVLYFVQDIFPHVLKKIPTIKFFIVGSNPPQEITALSSSNIVV
                     LGFVPDITDLMNQIRISINPLRFGAGIKGKLITCMSYGIPCIGTGIAAEGMSLTHEKN
                     ILIADTPSAFSDCIINLYSNPILWEQLSNEGLAFVQQNFSLNVAKEGFTQIFNRLRLK
                     NKTVK"
     gene            12337..13263
                     /locus_tag="PKZ49_02675"
     CDS             12337..13263
                     /locus_tag="PKZ49_02675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HND35494.1"
                     /translation="MIDMQNRVLELNPTISVIIPLYNHERYIDDAIDSLLQQTIPPFE
                     IIIIDDGSHDNSWQRVQHRAKEEPRIITWSQKNQGAHYTLNNGLNHATGEYLAILNSD
                     DVYHPQRLEFCLQAMQANPVPDVICTALAFINEKGKSCSNAWYEEALAFHRKVSDLSL
                     ALINGNFLMTTSNLFMHRSVWADLGTFANLRYAHDLDFFLRAILFNKRIVILENPLLH
                     YRTHATNTINEGVLKVKLEWAAVVAYYAWKMKRNDWSYWEQLVEITNRHDLTRMLFFF
                     FKYYQQNVTERLAVDDWLADKAFCKFLTDTIR"
     gene            13260..14513
                     /locus_tag="PKZ49_02680"
     CDS             13260..14513
                     /locus_tag="PKZ49_02680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HND35495.1"
                     /translation="MKILTISNLYPPNIVGGYEVLCFEVMRGLAEKGHEINVLTSDYG
                     GKQAIYPNQQVQRLLTLLATEGNIYKPLNISAIERNNINQKNLQTLSRTLDEINPDLI
                     FIWNLHFFDVSLLDALSLVKKKQVYLLSDNWLINFYNSDFVANYFSREVYVKRDRWYA
                     CWHRFKSRLFNGQKKRSMLGYAIFASRFMQNLYSRAGFEFANSKIIYHGISSGVEESL
                     PFAKRETFIANDELRLLFAGRVVDIKGVHTAIEAMPHIINALPNLKISLSIVGDMRDQ
                     QYVANLKKIIAELGVEERVCFIAPVEESKLFGLFQDYDIYLFPSLYEPFSLTLIHALR
                     AGIPTIASDVGGNPEIVFHEQTGLLFRPADARHLAAQVVKFVHNKNLRVTVSSGARDH
                     ANQFTFNRMINQIEDYLLYSLESNQ"
     gene            14510..15466
                     /locus_tag="PKZ49_02685"
     CDS             14510..15466
                     /locus_tag="PKZ49_02685"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HND35496.1"
                     /translation="MNILVLGARGFIGKHLCSALVAANHRVRGFDLQPVNGNWPDIPG
                     VEWTAGNLDHVSDVMTALDKIDLVFHLISTTIPQTSNENPLLDLQQNVGATLKLLELV
                     IKQPRKPRFIFLSSGGTIYGIPHIIPVPEEHPTDPLSAYGIAKLAIEKYLALYGYLYK
                     LNYSILRLANPYGEFQSHRSSQGVIAAFVHKALHHKPVEIWGDGSIVRDYLYIGDVIS
                     AMITLIDYDGPEKIFNIGGGKGHSILELLSVIKELFQHDIAYYHLESRACDVPINVLD
                     IKRAASILGWKPTTPLHEGIKQVIDYQRSVDFSLNDNSSKNR"
     gene            complement(15470..17563)
                     /locus_tag="PKZ49_02690"
     CDS             complement(15470..17563)
                     /locus_tag="PKZ49_02690"
                     /inference="COORDINATES: protein motif:HMM:NF025665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HND35497.1"
                     /translation="MLKLLLQNITSKHRQEKFIREVKTNGLLTAIKKAYILLRQLQKP
                     LSNQLPNIDTKSYFAQLFQQAFKSESYIPFRINQIQSLRSDIKLIAFYLPQFHPIKEN
                     DEWWGKGFTEWTNVTKAVPQFLGHYQPHLPDVLGFYDLRLKEVQQQQITLAKQYGIYG
                     FCYHHYWFNKKKVLETPLETVLNNPDLDFPFCINWANQNWTRRWDGLEQDILLAQQHS
                     AEDDIAFIADSARYMRDPRYIRIEGKPLLILYRPGLLPDARATAMRWRKWCRENSIGE
                     IFLALTHAFEHTKPNEIGFDAAIEYAPNTYPVKPITQQIIASGKMINPLYQGNIYDYN
                     EAASIGQNQIMPSYKKFRGLFPGWDNEARIPGRGTTYIDSSPLRFHQWLATLISLSQK
                     QFKPSEQFIFINAWNEWAEGAHLEPDRKFGFSFLESCRIAQQLEILSQQKNNLISQEN
                     YPKVAIVIHAYYPDIFDEILENLSSTDKYKIKLFITTPSYQVSLIENRLISHGMEYQI
                     LGVNNQGRDIFPLIKILKEIYQQHFSFIVKVHTKKSKHRTDGTIWRKDLFFKLLTKSM
                     LEKNIQYLVDHPKVGILAPEGHLVPMNHYIAANEKAIIELSARLGVEMETVMKLHFVA
                     GTMFTARIDALLPLLTLSFEDTDFGVEKGQLDGTLAHALERLISIGNHRIGYQIRTLS
                     GQTTSHYAHADVTSR"
     gene            17697..18611
                     /locus_tag="PKZ49_02695"
     CDS             17697..18611
                     /locus_tag="PKZ49_02695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HND35498.1"
                     /translation="MNLPSPVSLVIVNHNAGLLLTDCIRSAQSQVQEIIVIDNASTDT
                     SLAEIEQRFSSKEKQLLTIKRLDQNIGFAAACNIGIQETRSPFVLFLNPDCMLPVNAI
                     DRLMQVLQSDSQIGMVGGRLLNADGSEQGGGRRAIPTPWRALVRATGLYRLERFWPNL
                     FFDFHLHKQPLPLNPIDVEAISGALMLVRRDAIDEVGVWDEGYFLHCEDLDWCMRFRH
                     KGWRIVFVPDVQVLHHKGACSQARPLFVAWHKHKGMLRFYQKFFRDQYPSVLMWLVST
                     GVWFRLGAVVFFHYLSQLGRFCLRQRRG"
     gene            18604..19497
                     /locus_tag="PKZ49_02700"
     CDS             18604..19497
                     /locus_tag="PKZ49_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HND35499.1"
                     /translation="MVNQSIGVFGATSLVGKRLLPLLIQNGWEVTAFSRQKIDSEFIP
                     TTSAIKWCSLESLRENQLNFNLDQKIPYWFYLAPIWTLPAYFDFLSKQMVKKIIVLSS
                     TSIFTKVTSSDTSEIKIAKKLEEGEANLRNWAERHEINWIILRPTLIYGYGQDRNVCE
                     IARFIQRFHFFPLLGNANGLRQPIHADDVAAACYAAFLSQQVTNRVYNISGNELIAYR
                     DMVERIFVALDKSPILLKIPYWFFQLAIKLLKPFPRYSKWSIAMVERMGRDLVFDHSE
                     ASRDFGFMPRAFKLGKEDIPL"
     gene            19648..>21040
                     /locus_tag="PKZ49_02705"
     CDS             19648..>21040
                     /locus_tag="PKZ49_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HND35500.1"
                     /translation="MKAQKLPHSELAEILISFKRAFRTVGVFSAVINLLLLMPALYML
                     QLYDSVLTSRNEMTLLMLTLIMLGAYIFMGTLEFIRSFVLIRIGAQFDLKLNKRVYDA
                     AFEQSLRIGDSNAGQALQDLTNLRQFLTGNALFAFFDAPWFPIYILVIFLFNVWLGVF
                     ALFGTAVLIVLAYINEKISHKPLAEANAMAVASSALATNNLRNAEVIEAMGMLPNIQA
                     RWYKIHSRFLNLQAEASEKSGVVTAISKATSVALQSMMLGLGALLVLEDSISPGMMIA
                     GSILLGKAIGPVQLLISVWRQFGNTRSAYERLTKLLEENPARKPGMALPKPKGIIAVE
                     NVIAAPPGSKVAVIKGLTFSIAAGEVLGIIGPSGSGKSTLARLLVGVWPAASGKVRLD
                     GADVYQWNKDELGPHIGYLPQDIELFSGTVSENIARFGEVDAEQVILAAKRAGVHEMI
                     LNMPKGYDTPLGDG"
BASE COUNT         5948 a         3904 c         4489 g         6699 t
ORIGIN      
        1 aatgattaag aggactaact gcttcacttt acgttaagtt tagagataat cggggttaaa
       61 tagcgggagg atatggaaaa aaacgctcct ttttactaaa gttcattata cgcaaatcgg
      121 atgtttagga aatacgctgc taaacgatca aaaaaacatt tgattttatt tacaaataat
      181 cggggccccc cattagtaaa gaccggcgcg cgtattacta ttgcgtgatt tgctagtgcg
      241 tcatgatgat gatccgtcag ccgattattg tcgattttgt agtcctatgc ttttccttgg
      301 catagacgag gatattttca atttgccacg cagtccgaac tggtgatacc gcaccgccaa
      361 aacagccaac aaaggcgaca ttggagctgt cctgctgatc ccagcgcttc aattccgcca
      421 accaacctca acattcatct cttgatccag tctatacccg gtttgggaaa tataggcaaa
      481 tgaaatggca gatatagata aaacgattac tcccgaagaa tatagccagg aattctatga
      541 gaagtattgc aaataaatag tttttctaga gagcggctat caaaagttaa ttttgtaatg
      601 taacccccgc gtaaccccag aaaataaaaa agggttagcg attttgctaa ccctttgatt
      661 ttattggtag gccgtgcgcg attcgaacgc gcgaccaacg gattaaaagt ccgctgctct
      721 accggctgag ctaacgaccc gtaatttggc attataccaa ttcatggtac tttataaaac
      781 cctttatcat tatctaaatt tcactcgagt cattttcgag cgttaatttc gaaactaaac
      841 atctaaattt gtcacttgaa gggcattttt ttcaataaat tcacggcgag gctcaacgac
      901 atcacccatc aaagtcgtaa atatctcatc cgtcaaaata ctatcttcaa tttgtgcccg
      961 cagtagacga cgattattcg gatccatggt cgtttcccat aactgagcag gattcatttc
     1021 tcctaagcct ttgtagcgtt gaatattgat gcctttttta gcttcagcaa gtaaccaatc
     1081 gagtgcttct ttaaattctg taatggtatg ctcatcattg ccgcgcttaa ctactgctcc
     1141 atttgttatc aaaccttgta agacttgtgc agcttttttg atctgctcga aatcattgct
     1201 tttgacaaaa tcttgatcga ggtaactggt ttgaatattt ccatgatggg tgcggatcaa
     1261 ctttaagcga tattgctcga aaacatcatc aaattcaggg ttgatttgaa cagtttgaac
     1321 aattgcggca agctttgctg cgctttgctg tgcattatct tggttgctta aatcgatgtc
     1381 aggttccttg agcaaagcat acatgacgga acgatcgatc aatcggctca tgcggtcgat
     1441 aacagcttca gccagcagat attctcgtgc aatgctggtt aaagcttctc ccattatcgg
     1501 tggattgctt tcactaatct gtaattcagc gccttccagg gccaatccca agacatattg
     1561 attgagttca tgatcatctt taacgtagcg ctcttgtttc ccatgcttga tcttataaag
     1621 cggcggttgc gcaatgtaaa tatgaccacg ttcgatcaat tcaggcattt gacggtagaa
     1681 gaaagtcaat aataaggtgc gaatatgaga gccatcgaca tcagcgtccg tcatgataat
     1741 gatgcggtgg tatcgcaatt tatccggatt atattcatcc ttgccaatcc cggttcccaa
     1801 cgcggtaata agggaaataa tttcttgtga ggaaattaat ttatcaaatc gtgatttctc
     1861 gacattcaaa attttacctt ttaaaggcat gatggcttga aacttacggt ctctgccttg
     1921 ctttgcagaa ccacctgccg aatctccttc cacaagatag agctcacagt tagctggatc
     1981 tttttcctga caatctgcaa gcttacccgg taaaccaaga ttatcgagaa ccccttttct
     2041 tcgggttaat tcacgtgctt ttcgagccgc ctcgcgtgct cttgccgcat cgatgatttt
     2101 attgcagata attttggtat cgctgggatg ttccaacaaa aattcagtaa gcttttgtgc
     2161 aacgatatct tctacgacgg gacgaacttc tgaagaaacg agtttttctt tcgtttgtga
     2221 agaaaatttc ggttcaaata gtttgattga taaaacacag gttaaacctt cgcgcatatc
     2281 gtccccggta gtatcaactt tcgctttttt ggctaattca tttttttcaa tgtaactatt
     2341 aagcgtccgg gtcatggcag cacgtagccc cgttaagtga gtaccgccgt ctttttgcgg
     2401 gatattattg gtaaaacaca atacttgttc tacatagcta tcgttccact gtatggcaac
     2461 ctcaaccgca atattgtctt tactcccgtt cgcgtaaaaa attgctggat gtaatacagt
     2521 tttagtgcgg ttgatatatt caacaaaatt acgaataccg cctgaaaaag cgaatgtttc
     2581 ttctttgtca tggcgttgat cgatgagctt gatcttaatt ccgttattta agaaagaaag
     2641 ttctcgcaac cgtttagcaa aaatttcata gtgatagtta acatcaccaa aaatctcctt
     2701 actggctaaa aaatgaacct ccgttccatg cttttctgat tcacctgtta ctgccaatgg
     2761 agcgacagga acaccttttc gaaattccat ttgatggatt ttcttatcac gccgaatcgt
     2821 tagtctgagc cattccgaca atgcattcac gacagaaaca ccgacaccat gtaagccacc
     2881 agaaacttta taggaattgt catcaaattt accgcccgca tgtaattcgg tcatgacaat
     2941 ttcggctgcc gagcgtttca actcatcatc ttgtttgatt ccagttggaa taccacgtcc
     3001 attatcatgg acgctgacag agttgtcggg atgaatgatg acaatgattt catcacaatg
     3061 tcctgctaat gcttcatcga ttgcattatc aacgacttca aataccatat ggtgcaagcc
     3121 tgttccatcg ctggtatcgc cgatgtacat tccaggacgc ttacgaacag catctaatcc
     3181 tttcagaatt ttgatgctgt ccgaatcata ttctcgatcg tctttattgg tcattttgtc
     3241 agtcgtgctt ttaagagttg atcaaattga attactagag tcgcatgggc attacgacat
     3301 atttgaaagc ttcatcacct ggaatggtga ttagtgtgcc gctatttgca ttttcaaagg
     3361 aacattgaat tgtctctgaa tcgagattat taagtacatc gagcaaataa gtaatgttaa
     3421 agctaatgtc gatgatttcc tgactattat agtcaatatc caaatcttct tgcgcttcct
     3481 cttgttcact atttttgcaa aggatgctta aacgattatt tgcaataata agtttcacgc
     3541 ctctaaattt atcactcgta ttagaaagaa tggcagcgcg ctgaagtgct tggagaaaat
     3601 ccaggcgttt gattataaac tgctttgtat tattttttgg gataacacgg tcgaaatctg
     3661 ggaattttcc atcaatgatt ttagaaatta aaatgatgcc agaaaagcta aagctgatct
     3721 ttttagaata aaagtcaatc gttaccgtct tatcactatc ttcgagtaat tttacaagct
     3781 catgaaccgt tttacggggg aggattgttt cttttttgat tagctccgct tcaaactgca
     3841 tggagatata gccaaggcgg tgaccatcgg ttgcaacagc tttaagttgt tgaccgtcag
     3901 ttagcagtaa caaaccattt aaatagtagc ggatatcctg ctgtgccatt gcatattgaa
     3961 catggtacaa gcaatttttt aaatcttttt gcgacaacgt tagggaaacc tctggctttt
     4021 cttcgatcgc cattcttgga aaatcttcca tcggaagaat ttgaagattg aagcgacttt
     4081 tgctgatatt aacttgtaag cgattatcag ttttgttaag aacgacactg gtatcagcag
     4141 aaaatgcgcg aaggatatcg aggaattttc ttgctgatac tgttattgaa aagttttctt
     4201 tggaaagatt ttcattatcg atcgagtgta cttcgatttc cacttccaga tcagtggtaa
     4261 cgaaaaaaat tttttcttgt tccttacgaa tcaatacgtt tgaaagtatt ggcaaggttc
     4321 gtttttgttc aacaatgcca cttacaattt gtagcggttt aagtaatgtg tcacgttttg
     4381 tattaattaa attcatttaa tttaaaacta aataataata gataaagagg gcttaattcg
     4441 gggatatgaa ataataaata ttaaaaatca aagacatgtt taaataatat tggctgtata
     4501 agttaaaaat ataaaaggga taaattgtgg ataattctta tttaattttt aaatgatgag
     4561 ttatccacaa ttgatacatc actattatta agttttattc cttagttatt atcccctcaa
     4621 aatatgaatc agcgcattgt aatcccgggt aatcaaagga tctgatgcgc gtaattcatt
     4681 gattttgcgg tgggcatgta atacggtagt gtgatctctg ccaccaaaag cttcaccaat
     4741 gtccggcagg cttaattgtg ttagttcttt tgcgattgcc atggcaattt gtctcggtct
     4801 tgctatcgtt cgcgttcgct ttttagaata catttcagca actttgattt tgtagtaatc
     4861 cgcgacagtt ttttggatat tttcaatcga gatttgtctg ttttgaacgg caagaagatc
     4921 ttttagggct tcttttacta attccaggga taaatcctgg cctgtaaaac gagaataagc
     4981 caatacgcgt ttcagcgcgc cttcaagttc acgaacattc gaacgaatgt gtttcgcaat
     5041 gaaaaatgcg atattttctt ctagtgaaat tttttctagc gctgcctttt tgattaagat
     5101 tgcgacccgc atttctaatt ccggcggttc gatcgcaaca gttaaacccc agccaaatcg
     5161 cgatactagt cgttcttcta atcctgagat ttcttttgga taacaatcgc atgtaatgat
     5221 gacttgcttg gatgcttcaa ttaaagagtt gaaagcatag aagaattctt cttgggttcg
     5281 attttttcca ccaaagaatt gaacgtcgtc cactaatagt acatcaaggg aatgataata
     5341 ttttttaaat ttgtcgaacg atttatgttg gtaagcactt acaacgtctg aaacatattt
     5401 ctctgcatgg acatatctaa ttttcgcttc gtgattgcgt tctaagataa aattaccaat
     5461 cgcttgaatt aagtgagttt ttcctagccc aacgccacca tatataaata atggattata
     5521 agcagtacct ggtctttcag cgacttggat agcgccagct cgcgccagtt gattggcttt
     5581 tcctgtaacg aaattatcaa aagtaaaatt cgggtttaga taatgcttat tactcgcttt
     5641 tttggagggt gtcggatcag caacggaaac ggttgaaacc gcttgatctt cccgtgaggt
     5701 gtcgatgatc gggtttactt taggagtagc ttttttggta gtgatttcac caagcacgag
     5761 ttgaaattga atccttttag aaaaatgttg ctgcgccata gcttcgatat gagggataaa
     5821 gttgtcttta acccattgaa gtacaaaccg attaggagca atcaaaatag gttggtcgcc
     5881 acaaccttcc gagatatcga gctgcaatgg tttaatccat gtattgaatt gctgagcatt
     5941 taattccttc tcaaaatgct taagacatgt ataccagaat gcttcaaatt cctgcatgga
     6001 ctaacgatgt gaattggact caataaacgt gcttatatac attctacact gatgtttgat
     6061 ttcacgttgg tagttcatcg caatgatcgg ttaattcttt gtttttgaac gtatttagtt
     6121 aattttattg aagtaatgaa cttgttaata actttagaag ttaaaataag ctaattcatt
     6181 ggttaaatca tgaaacaatt tatactggaa actgctttaa gggtaatttc aatcaagcct
     6241 tgtatgttca tgatcaaata ctgtgacact taaacctgcc gttcttgctc atcatttgag
     6301 taaatgttat caactttata cacagcccaa agatcgtttg aaacaattga tctggggtcg
     6361 gcgtcgtcag tattatcgag agttgtgggc attgcgtgag attaatcttg caattatgcc
     6421 aggtgaagtg atcggaatta ttgggaaaaa tggttcgggt aaatcaacct tattgcagtt
     6481 aatttgtgga acgctaacac cgacaacagg agaagtagag atcaatggtc gtttagctgc
     6541 gttgctcgaa ttgggcgcag gctttaatcc tgagtttaca ggtcgagaaa atatctggat
     6601 gaatgcagca ataataggac tgacacaaca agaaattgcc gaccgaattg aacaaattat
     6661 tgatttttcg gaaattcgtc cttttgttga tcaacccgtt aaaacttact ccagcggaat
     6721 gtatgtaagg ttggcttttt cgatcgcaat cagtgttgat ccggacattt tgattatcga
     6781 tgaggctttg tcagttggag atggcgcttt cgcgcgcaaa tcatttaatc gtatcatgca
     6841 gttacgggat agcggcaaaa cgattttatt ttgctcccac tcactttttc agattgagtc
     6901 attgtgtaat cgtgcatttt ggatcaatca aggagaattg atagcagaag gtgatgtaaa
     6961 gcaagttgtt acggcttatc aacaattcct ggatctgggt gtattaagca gcgataataa
     7021 tgatattgcg gcgactattc agcagaatga ttatcttgct aatgcaaagg gctatgcaag
     7081 gcttgaaaca gtggtcgttg aaattgatgg agcaccctgc gctgtggcgg tagccgaaag
     7141 tgggcaatcg gcgatccagg tcgatgtcaa attttctacg gatccctcgt taccctgtcc
     7201 gacagttgca gttacacttc attctatgga tggtcgagta ataacaagcg ctgcgagttg
     7261 ggaagatagt atcatccctc gattaacgcc agaaggaata ggggaaataa aactcctctt
     7321 tgaacaactt cctttactca aaggtgaata tttagtcagt gtgtacttat tatgtgagca
     7381 gggaattcat ctctatgatt ctgtttacgg cgccacgaaa cttcaagtcc agcaacaaaa
     7441 tcgattacaa ggttttttta tgattcctca tcaatggaag taaattgagt gatggtattt
     7501 tggggatcat ctcgtctatt taattacagc caaagtttaa cgtgtcttca atatctaatt
     7561 gcatgaatgg attttcatct aagcaagaga tcagtgatgg gtctattaca atcaatagcc
     7621 catcgttatt taaaaacttc gattttccgc ttaatgtcta tgcgcacaca ctttttttaa
     7681 atgaaggtag ggtagattat cttcattatg ggttgtttga atcagaacag attaatttaa
     7741 gccaagcaca acaatattca accaatttga ttctggatcg tttaccactt ccccctagtc
     7801 gaattctgga ggttggtgca ggtttaggta cgacttgtca tttgttgacg caacggagct
     7861 atcaagttca cagtattaca ccggatcgtc agcaaattgc cgaaattcat cgacggtttg
     7921 cgactgggct caatgtaacg cagcaacgtt atgaagattt ggagcttatt actgaaagct
     7981 atgatgtcat tttgtttcag gaatccgctc agtatatcga accgctcgtg atatttaaca
     8041 aagcgcaaga tttattacca gaagaagggc atattttcat tattgatgag tttgcattgc
     8101 agcaatcgga aactgcgatt ggaagacttc accgattgaa agctatgtta cagttgtcag
     8161 atcgtttagg cttcgagtta gtggaacatt tggatttatc ctcaaaagca acgcctacat
     8221 tgaagtattt actgcatgcc attggcaagc atcggcagca attgctggat gatcttgatt
     8281 tggaatcgag tcgtctcgat caattagtgc agtccaatca agattatcta aataaatatg
     8341 tatcaggtca atttggctat gcgctattac attttcgaaa aaaacgccaa ttaccatggc
     8401 gactgtcccg gcttaacacc aaccaaatgc cccatatgct cgcgttattt gagaaaatat
     8461 ttaagcacaa gatgacgtcg gaaatgtggc aatggaagta tggttcggag caatgtcgag
     8521 caatgggtgt ttggtatgat aatgagctaa ttgcgcatta tggtggaatg ggtcgaagca
     8581 tactttattt cggccaaccg caaatggccg tccaaatcgg tgatgtcatg gttgacaggg
     8641 taaatcatcc tggattagta aaaaaaggcc ctttttttca gatggcagca acattcctgg
     8701 aacactatat cggttatggt aaaccttatt tattaggatt tggttttccc aatgataggg
     8761 cgatgaaagt tgctaaacgt cacggattgt atgctgaaac ggggcgtatg gtggaaatag
     8821 catggactcc ttattcaagg cgacctttgt ggctttctca attgaaaatc atcgatcagc
     8881 ataatatcaa cgataattgg gtagtcgatg ccattaatca gtgttggcat ggtatggcat
     8941 ctgatcttaa tgcagcgatt ataggaatac gcgattgggc ctacctacgt gatcgttatt
     9001 tgaatcatcc tcaacatcat tatcgtattg tgctcgtcct gagtcggttg agtagaaaag
     9061 cgaggggaat tctggtactc caatctaatt caaatggttg tgaaatcatg gatatggttg
     9121 caccactcga tcagatttcc ttgctgataa ctcatacgag acgaattgca cggattaatg
     9181 gtgatcaacg tgtattttgc caaattaccc ataatttctc tgatcgtttt atttcagcga
     9241 gtggtgaagg aatgttgagt gagttaccca ttcgaatacc cgcaaatgct tggagtgatg
     9301 gtccatcacc acaattactc atgaatcact ggtggttaat gagtggtgac atggatttta
     9361 ggtagtgcac tatccattaa tttttgtata aaaaatattc tcattggaaa atcttctcat
     9421 accttatcaa gcgacaacaa taataccgtg caagaaagtg ctggtgtttg cccctcaccc
     9481 cgatgatgaa gtatttggat gtggcggcgc gattatgcgg catcttgcac aaggcgttgc
     9541 tgtggaggtg attatcgtct ctgatggtgc gtatggagca aaatcggaag aaagttcaag
     9601 gcaatatgtg ctacagcgtc aagaagaaag taacttagca gcaaagattt tagggtacgg
     9661 tcttccatca ttctggtcat atccagatcg taaggttggc tatggagaaa agctcatcca
     9721 agacatttta gctgctatca atcaatccca agcagattta atctatgcgc cctctgtttt
     9781 cgaggtgcac cccgatcacc gttcgatagg gatggctgcg attgaagcag tcagaagaat
     9841 aggtaaatca ctgaagctcg cattgtacga agtgggtatg ccgatgcgac ccaattgctt
     9901 actcgatatc agcgaattcg ttgagcgtaa aatggctgca atgcagtgct tcagctcgca
     9961 aaaccagaag cagcgctacg atctcgatat tgcggcactt aatcgctatc gaacctatac
    10021 tttgccagct gaaattcagg cagcggaagc ctatttgata atttcggccg aagaattggt
    10081 taaggatcca ttaaagcttt atcaatccga atatgcgcgt caaagagagt tgggagtagc
    10141 gatcgatcgt ggtgatattc ctttagtgag tgtcattatt cgaagtatgg atcgacctac
    10201 gttaacgaga gctttggata gtttagcgtt acaaacgtat cccaatattg aggttgtttt
    10261 ggtgaatgca aaaggcggga tgcacgagca atcgtctgat tggtgtggaa attttccgct
    10321 tcgtttaatt aaccaggatg gtgaaccttt gcttcgctct aaagcagcga atattgggtt
    10381 agatgcttgt caaggtagct atataagttt tcttgatgat gatgatgcga tcgatccgga
    10441 tcatattgct aatttgattg agggaatcca gtcagtatcg acatgctcaa ttgcatatac
    10501 cggtgtgcgt ggtaaagatg agcaggatga aaataagccg tttgttgaat ttaaaacggc
    10561 tgaagtcaat tttgcaaaat tgttgctagg gaacgttatt cctatccatg caatcctctt
    10621 tccagcaacg ttgttaaaac aaggcgcagt gtttgatgaa aagctagctt tatacgagga
    10681 ttgggatttt tggctacaac ttagccggta cacccccttt aattttataa atcgaacgag
    10741 cgctacttat tttacgggtg gtggatcgga agtttctccg ctgcacaatg atcctgttcg
    10801 agtaaagcaa gcaacggtta acctgtatca aaaatggctg aaattactta cagcaagcga
    10861 attcgcagcg gttgccgatt tgcatcatcg aaccaacaat caatttcaag ctgaaatggg
    10921 cgggttacgc agtgaaatta gcagattgca agacttacaa cagcaaacac gttttcaatt
    10981 agagcaaaaa cagcaggagc tagatatttt atatgcgtca cgttcctggc gtctcacact
    11041 accattgcgc tgctgtttgg agcgcgttag agcattaaaa agtgtattta agcggtgcta
    11101 ttacacactt ccaggcgcat acagaattta tcgttttgtt agggaattca gtcagcagcg
    11161 tagaaggcaa tcgagttctc aaattgatca aagtggggga ccctggccaa ttggacctgc
    11221 tgggcaaatc ctgttgattg agcacagtgt gcctcgacca gatcaggatg ctggttctat
    11281 gatgatctat aactttatca aagtttttat tgagatggga tatggtgtaa cattttatcc
    11341 agttgattta gcttatgatc cagtctatac accgcgattg attgaactaa aagtacgatg
    11401 tttaaataag cctcaagtta atgcattaga gcatcatttg gctgcgatag atcgttctta
    11461 tgatttcgtt ttatcatgcc gccctgtcta taccgaagca ttgataccta taatcaaaaa
    11521 gtattgcact acggcgcatt tcatttatga aacacatgat ttgcatttta ttcgtttaca
    11581 acggcaggcc gaggttgaac ataatcgcga gatgttggcc cattccagct ttctcaaagc
    11641 aatggagtta aatatcgcgg ctgaagctga ctgtactatt gtggtgagtc ataaagagaa
    11701 ggaaattttg ttgcaagaaa accccaatct ttatatcgaa gtaattccgg ttgtcagcga
    11761 ggtgtttggt cgtaagagtg actttacaca acgcaatgat ttggtattta ttggaaacta
    11821 tgagcatcgt ccaaatgtag atgcagttct ttattttgtg caagatattt ttccacacgt
    11881 attgaaaaaa attcctacaa tcaaattttt catcgttggc agtaatcctc cacaagaaat
    11941 tactgcttta tctagtagca atattgttgt tctaggcttt gtgccagata taactgattt
    12001 aatgaatcaa attagaatat ccattaatcc tttacggttt ggcgctggaa taaaaggaaa
    12061 gctgatcact tgtatgtctt acggtattcc atgcattggc acaggcatag ctgctgaagg
    12121 tatgtcgttg acgcacgaaa aaaatattct gatagctgat acaccttcgg cgttctctga
    12181 ttgcattatt aatctttatt ccaatccgat tttatgggaa cagctatcaa atgaaggact
    12241 tgcatttgtt cagcaaaatt tttcactgaa tgtagcaaaa gaaggattta cccaaatatt
    12301 taatcgtttg cgattaaaaa ataaaacggt taaataatga ttgatatgca aaaccgtgtt
    12361 cttgaattga atcccacaat ttcagtgatc ataccgcttt ataaccatga gcgatatatt
    12421 gatgacgcga ttgatagctt gttacaacag acgatacctc catttgaaat tatcattatt
    12481 gatgatggtt cacatgataa ttcctggcaa cgagtacagc atcgcgcaaa agaagaacca
    12541 cgtatcatta cttggtcgca gaaaaatcaa ggcgcacatt acacgctgaa taatggcctt
    12601 aatcatgcaa ctggtgagta tttagccatt cttaattcag atgatgttta tcatccacaa
    12661 cgccttgaat tttgcctgca agcaatgcaa gccaatcccg ttccggatgt aatttgtaca
    12721 gcactcgctt ttattaatga aaagggtaag tcttgtagca atgcttggta tgaagaagcg
    12781 ctagcgtttc accgaaaagt cagtgatctt tcattagcac tgattaatgg taatttcttg
    12841 atgacaacct ctaacctatt tatgcatcga tcggtatggg ctgatctggg tacctttgca
    12901 aatcttcgtt atgcgcacga tctcgatttc tttttgcggg cgattttatt taataaaaga
    12961 attgttattc tagaaaatcc attattgcat taccgaacgc atgctaccaa taccatcaat
    13021 gaaggggttc tcaaagtcaa attggaatgg gctgctgtgg tagcctatta tgcttggaag
    13081 atgaagcgaa atgattggag ctattgggag caactggtcg aaataaccaa tcgccatgat
    13141 ttgacaagga tgcttttttt cttttttaaa tactatcaac agaatgtcac tgaaaggctt
    13201 gcggttgatg attggctcgc tgataaagct ttttgcaaat ttcttactga cacgattcga
    13261 tgaaaattct tactatcagt aatctttacc cccctaatat agtcggtggc tatgaagtac
    13321 tgtgctttga ggtaatgcgg ggactggctg aaaaagggca tgaaatcaac gtgctgacca
    13381 gtgattatgg cggcaaacaa gcgatttatc cgaatcagca agtacagcgc ttacttactt
    13441 tacttgccac agaaggtaat atttataaac cattgaatat ttctgcgatc gagcgcaaca
    13501 atattaatca aaaaaatctg cagacattgt ctagaacgct ggatgaaata aacccagatc
    13561 ttatttttat ttggaatctg catttttttg atgtgtcgct gctcgatgca ctttcattgg
    13621 ttaagaagaa acaggtttat ttgctttctg ataattggct tatcaatttt tataatagtg
    13681 attttgttgc taattatttt tctcgtgagg tttatgtaaa gcgcgatcgt tggtatgctt
    13741 gttggcatcg ttttaaaagt agactcttta atggtcagaa gaagcgaagt atgctgggtt
    13801 acgctatatt tgctagccga tttatgcaaa atctttattc tcgtgctggg tttgagtttg
    13861 caaacagtaa gattatttat cacggtattt catcaggagt agaagaatcg ttaccgtttg
    13921 ctaaacgcga aacgtttatt gctaacgatg agctacgtct attattcgct ggacgtgtag
    13981 tggatatcaa aggtgtccat acagcgatcg aagccatgcc ccatataata aatgcgttac
    14041 caaatttgaa aataagccta tctatcgtag gtgatatgcg cgatcaacag tatgtggcta
    14101 atctgaagaa aataattgcg gagcttggag tagaagaaag agtttgcttt atcgcaccag
    14161 tagaggaatc gaaactgttt ggtctttttc aagactacga tatttacctt tttccctctt
    14221 tatatgagcc tttttctctt acgttaattc atgcacttcg ggcgggtatt cccacgattg
    14281 catcagatgt tggcggcaat cctgaaattg tttttcatga acaaacgggt ttattgtttc
    14341 gcccagcaga tgctagacat ctggctgctc aggtagttaa atttgtccat aataagaatt
    14401 tgcgggtgac tgttagttct ggagctcgag atcatgctaa tcagtttact tttaatcgaa
    14461 tgattaatca gatcgaagat tatttgttgt attcgctaga aagtaaccaa tgaacattct
    14521 tgtactcggg gcaagaggtt ttattggaaa acacctatgc tcagctttag ttgctgcgaa
    14581 tcatagggtt agaggttttg atttgcagcc agtcaatggt aactggccag atattcctgg
    14641 tgttgagtgg actgcaggaa atttggatca tgtgtcagat gtaatgactg ccctcgataa
    14701 gatcgattta gtatttcact tgattagcac aacgattcct caaacatcta atgaaaatcc
    14761 tttattggat ttacagcaga atgttggtgc taccctgaaa ttacttgagc ttgtaattaa
    14821 gcaaccgaga aaaccgcgtt ttattttcct ttcatcgggt ggaacaatat atggaattcc
    14881 tcacataatt cctgtaccag aggaacatcc aaccgatcca ctcagcgctt atggtatagc
    14941 caaattagcg atcgaaaagt accttgcact ctatggttat ttgtataagc tcaattatag
    15001 tattctgcga cttgctaatc cttatggtga atttcagtcc catcgttcta gccagggcgt
    15061 catagctgct tttgttcata aagcattaca tcataagcct gttgaaatct ggggggatgg
    15121 gagcattgtt cgcgactatc tttatatcgg tgatgtcatt tctgcgatga ttaccctaat
    15181 tgactatgat ggacccgaaa aaatattcaa cattgggggt ggcaagggtc atagtattct
    15241 tgagctttta agcgttatta aggagctatt tcagcatgat atcgcgtatt atcatcttga
    15301 aagtcgcgcg tgcgacgttc cgattaatgt tttagatata aagcgtgcag caagtatctt
    15361 agggtggaag ccaacgactc cattgcatga aggaataaaa caggtcattg attatcagcg
    15421 ttcagtagat ttttctttaa acgataattc ttcaaaaaac aggtaatatt tatctggagg
    15481 ttacgtcggc atgcgcgtaa tggctcgtgg tttggccgct aagtgttctg atctgatagc
    15541 ctatgcggtg atttccaatt gatatcaaac gttcaagtgc atgggccagg gttccatcca
    15601 attgcccctt ttccactcca aagtctgtat cctcaaagct caatgttagc aaaggaagca
    15661 atgcatcaat tcgcgcagtg aacatggttc ctgctacgaa atgtagcttc ataacggttt
    15721 ccatttcgac acctaatcga gcagataatt ctatgattgc tttttcattt gcggctatat
    15781 agtggttcat gggaacaaga tgaccttctg gagctaagat accaacttta ggatgatcaa
    15841 ccaaatattg aatgtttttt tctagcatag attttgtaag taatttaaaa aataaatctt
    15901 tacgccagat agttccatct gttctatgct ttgatttttt tgtgtgaact tttactataa
    15961 atgagaagtg ttgctggtag atttctttta agattttaat aaggggaaaa atatcgcgtc
    16021 cttgattgtt tacacctagg atttggtact ccatcccgtg agatataagt cgattttcta
    16081 tgagtgatac ttggtagctg ggtgttgtga taaataattt gattttatat ttgtccgtac
    16141 tacttaaatt ttcaagtatt tcatcgaaaa tatctgggta gtaggcatga ataactatag
    16201 ctacttttgg gtaattttct tggctaatga ggttgttttt ttgctgactt aaaatttcta
    16261 attgctgagc aatccggcaa ctttcaagaa agctaaatcc aaattttctg tcgggttcga
    16321 ggtgagcgcc ttctgcccat tcattccaag cattaataaa aataaactgc tcactcggtt
    16381 taaattgctt ttgagaaagt gagattaagg tagcaagcca ttgatgaaat ctgagtgggg
    16441 aggaatcgat ataagttgtt ccacgtccgg gtatgcgcgc ctcattatcc cacccaggaa
    16501 ataatcctct aaatttttta tatgagggca tgatttgatt ttgtccaatc gacgcggcct
    16561 cgttgtaatc gtatatattg ccctgatata gcgggttaat catttttccg gaagcaataa
    16621 tttgctgtgt tatcggtttt accggatatg tattgggtgc atactctatt gccgcatcaa
    16681 agccgatttc atttggtttg gtgtgctcaa atgcatgagt gagtgctaga aagatttcac
    16741 caatcgaatt ttctcgacac cacttgcgcc aacgcatggc ggttgctctc gcgtcaggaa
    16801 gcagtccggg gcgataaaga attaataaag gcttgccctc gattcgaatg taacgaggat
    16861 cgcgcatata ccttgctgaa tcagcgatga aagcaatatc atcttcggca gaatgttgct
    16921 gggctagtaa aatatcttgc tctaggccat cccaacgtcg ggtccagttt tgattggccc
    16981 aattgatgca gaaaggaaaa tcaagatctg ggttgtttag aacagtttca agcggagttt
    17041 cgagaacttt ttttttgtta aaccagtaat ggtgataaca gaatccataa attccatact
    17101 gcttggctaa tgtaatttgt tgctgctgta cttcttttag gcgtaaatca tagaaaccca
    17161 gtacatcggg taagtgcggt tgataatgcc cgagaaattg tggaaccgct ttggttacat
    17221 tcgtccattc ggtaaaacct tttccccacc attcatcatt ttctttgatt ggatgaaatt
    17281 gtggaaggta aaatgcgatt aatttaatat cagaacgcag agactgtatc tgattaattc
    17341 taaaaggaat atagctttct gatttaaagg cttgctggaa tagctgagca aaataagatt
    17401 tcgtatcaat attagggagt tgattggata agggtttttg taactgccgc aacaagatat
    17461 atgctttttt aattgcggtt aacaaaccat tggttttgac ttcccgtatg aatttttctt
    17521 gtcgatgttt gctagtaata ttttgaagca ataatttgag cattgattga agctgtggtt
    17581 ttacgaattt gcttataaat aaacggttga gctatataat ggagattaga tttatactac
    17641 ttgcgattgg cttggatgta gattgattga atatgcagca tcttattaat gaataaatga
    17701 atcttccatc accggtatcg cttgtgattg taaatcataa tgcaggattg ttactaactg
    17761 attgcattcg ctcagctcaa agtcaagttc aggaaattat tgttatcgat aatgcttcca
    17821 ctgatacgag tttggctgaa atcgagcaaa ggttttcaag taaagaaaaa caactgctca
    17881 cgattaaacg cttagatcaa aatatcggat ttgctgctgc ctgtaatatt ggaattcaag
    17941 agacccgatc cccttttgtt ttattcttaa atcctgattg tatgcttcca gtcaatgcga
    18001 ttgatcgtct catgcaagtg ctgcaatccg atagccaaat cggaatggtt ggaggccgac
    18061 tccttaatgc ggatggaagt gagcaaggcg gtggtcggcg agccattcca acaccttggc
    18121 gtgcactggt gcgagcgacc ggtttatatc gcttggagag gttttggcct aacctttttt
    18181 ttgattttca tttgcataaa cagcctttac ccctaaatcc tatcgatgta gaggctattt
    18241 ctggtgcatt gatgttggtg cgccgtgacg caatagatga agtgggtgtt tgggatgaag
    18301 gttattttct tcattgcgag gatctcgatt ggtgcatgcg gtttaggcac aaaggatggc
    18361 gaattgtctt tgttccagat gtgcaggtat tgcatcataa aggcgcatgt agccaagcta
    18421 ggccgctctt cgttgcttgg cataagcata aaggtatgtt gcggttttac cagaaatttt
    18481 ttcgtgatca gtacccttcc gtgttaatgt ggcttgtctc tacaggtgtt tggtttcgtt
    18541 tgggggcagt agtttttttt cattatcttt cccagctagg gcgtttttgc ttgagacaac
    18601 gacgtggtta atcaaagtat tggtgttttt ggagcaacta gtctggttgg aaagcgctta
    18661 ttaccactac taatacaaaa tggttgggaa gtgacggcat tttcgaggca aaaaattgat
    18721 tctgaattta tcccgaccac ttctgcaatt aaatggtgct cattggaatc attgcgtgag
    18781 aatcagctta attttaattt agatcagaag ataccctatt ggttttattt ggcgccgatc
    18841 tggactttgc ctgcttattt cgatttttta tctaaacaaa tggtaaaaaa aataattgtt
    18901 ttatcttcta caagtatttt taccaaagtg acttcctcgg atacaagcga aataaaaatc
    18961 gccaaaaaac ttgaggaagg tgaagcaaat ctacgaaatt gggctgagag gcacgagatt
    19021 aattggatca ttttacgccc tacccttatt tatggttatg gccaagatcg aaatgtttgt
    19081 gaaatcgctc gcttcattca acggtttcat tttttcccct tattaggaaa tgcaaatggc
    19141 ttaagacaac ctatccatgc agatgatgtt gctgcggcat gttatgctgc atttctatcc
    19201 cagcaagtaa ctaatcgagt atataatatt agcgggaatg aattgattgc ctatcgggac
    19261 atggtcgaac ggatatttgt cgcattagat aaatcgccaa tcttgctcaa aatcccgtac
    19321 tggttttttc aattagcaat aaagctgtta aaaccatttc ctcgatacag caaatggtct
    19381 atcgcaatgg ttgagcgaat gggaagagat cttgtatttg atcattcgga agcctcgcga
    19441 gattttggtt ttatgccgcg cgcgtttaag ttaggtaaag aagatatccc actttagcca
    19501 tcatgcgcat caagaatctg tgagaaatta cacattcgca ttaaggtatt ttttggttta
    19561 atggccgaca ttcttgactt ggcttgattg gcactattac caagagatca tctgtaagac
    19621 aaatcgctta tttttctaat tttttaaatg aaagcacaga agttacctca tagtgaactt
    19681 gcagaaattt taattagttt taaacgggct tttcgcacgg ttggtgtgtt tagtgcggtc
    19741 ataaatttac ttttgttaat gcctgcgttg tatatgttac agctttatga cagcgttttg
    19801 actagccgta atgaaatgac cctcttgatg ctgaccctaa ttatgttagg ggcttatatc
    19861 ttcatgggaa cgttagagtt tattcgaagt tttgtgctga ttcgaatcgg ggcccaattc
    19921 gatttgaagt tgaataagcg tgtctatgat gctgcctttg aacaaagtct gcgaattggc
    19981 gatagcaatg caggccaagc attacaagat ctaaccaatt tacgtcaatt tttaacagga
    20041 aatgcattgt ttgctttttt tgatgcgccc tggtttccca tttatatatt ggttattttt
    20101 ctatttaacg tttggcttgg tgtgtttgca ctatttggaa cggccgttct tatagtgctt
    20161 gcttatatta atgaaaaaat atcgcacaaa ccgcttgctg aagcgaatgc catggctgta
    20221 gcttcttcag cacttgcaac caataatctc cgaaatgcag aagtaatcga agcaatgggg
    20281 atgcttccaa acatccaggc gcgctggtat aaaattcata gccgatttct taatttacaa
    20341 gcagaagcaa gcgagaagtc aggcgtcgtt actgccatta gtaaggcaac ttcagttgct
    20401 ttgcaatcaa tgatgctagg tttgggggca ttgctcgtac tcgaggacag catttcgccc
    20461 ggtatgatga ttgcgggctc tattttgctt ggtaaagcga tagggccagt gcaattattg
    20521 attagtgttt ggcgacaatt tggtaatacc cgcagtgcct atgaaagatt gacaaagtta
    20581 ttggaagaaa acccagcgcg taagccaggt atggcattgc ctaaaccgaa agggataata
    20641 gcagttgaga atgtaatcgc tgcaccgccc ggttcaaaag tggctgtcat caagggattg
    20701 accttttcga ttgcggcggg tgaagttctg ggtattattg ggcccagtgg atctgggaaa
    20761 tctaccctgg cacggttact cgttggtgta tggcctgctg catctgggaa agtacggtta
    20821 gatggtgcgg atgtttatca atggaataaa gatgagctag ggcctcatat cggttattta
    20881 ccccaagata tcgaattatt ttctggaact gtgagtgaga atattgctcg ctttggcgaa
    20941 gtcgatgctg agcaggtcat attggcggca aagcgtgcgg gtgttcacga aatgattttg
    21001 aatatgccca agggttatga tactcccttg ggagatgggg
//
LOCUS       DAOQKQ010000024        20924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_1102_length_20924_cov_6.474148, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000024 DAOQKQ010000000
VERSION     DAOQKQ010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 20924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20924
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1102_length_20924_cov_6.474148"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PKZ49_02710"
     CDS             complement(<1..228)
                     /locus_tag="PKZ49_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase system-associated protein, TIGR04073
                     family"
                     /protein_id="HND35501.1"
                     /translation="MAKDYPTKVGEKLGTGIANVVTGVAEVPKTMMITGRKEGATYGM
                     TAGLFIGIIHMAGRLFTGALDIATFPVPTTPI"
     gene            complement(366..2051)
                     /locus_tag="PKZ49_02715"
     CDS             complement(366..2051)
                     /locus_tag="PKZ49_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HND35502.1"
                     /translation="MTTSSTASNFIRNIIEDDNRSGKWNQRVETRFPPEPNGYLHIGH
                     AKSICLNFGLAKDYAGFCHLRFDDTNPEKEAQEYVDAICDSVKWLGFDWGKHIYYASD
                     YFEQLYAFAEFLIENGKAYVDSSSAEEIRQLRGTLNQPGINSPYRDRTIKENLDLFRR
                     MRAGEFPDGSHVLRAKIDMSSPNINLRDPVIYRIRHIHHQRTGDKWCIYPMYDFTHCI
                     SDALEKITHSLCTLEFEDHRPLYDWVLDQLQDKVPCHPQQIEFARLNLTYSVMSKRKL
                     IELVENKLVDGWDDPRMNTLAGVRRRGYTPEAIRLFTERIGISKADSWIDMTVLEDCL
                     REDLNERALRRIGVLNPLRLIIDNYPENQEEECYAPNHPQRPELGKRTLSFTKTLYID
                     QDDFMENPSKGYFRLSPGAEVRLRYAYIVKCTSIIKNDRGQIEAIHCTYDPDTKSGTP
                     GADARKVKGNIHWLSSKYARSAEIRLYDRLFTQPYPDAGEQDFKTYLNPDSKKIAMAY
                     LEPSLFEVQPEQIFQFERHGYFIADRVDTSPGKPVFNRAVTLRDTWGKIAGQG"
     gene            2232..3740
                     /gene="lysS"
                     /locus_tag="PKZ49_02720"
     CDS             2232..3740
                     /gene="lysS"
                     /locus_tag="PKZ49_02720"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HND35503.1"
                     /translation="MTEESELTPSQDENHIIAERRAKLAELRKRANPFPNHFRRDSFA
                     MSLQDTYCNQTKEDLEGQQISVKVAGRMMFKRLMGKASFATIQDMSGKIQLYVSNDHT
                     GEAAHEAFKHYDLGDILGAQGTLFRTKTGELSVRVTHLQLLTKSLRPLPEKFHGLTDQ
                     EQKYRQRYLDLITNENTRKVFIVRSKVIQAIREFLVARDYLEVETPMMHPIPGGATAR
                     PFSTHHNALDMPLYLRIAPELYLKRLVVGGMEKVFEINRNFRNEGISTRHNPEFTMLE
                     FYEAYQDYTYLMDLTESMLREVARAVMGTTHITYQEQEMDLAKPFAKLTITQAIQKYF
                     PEYSTAQLNDREFLISRLQALNIAYKPEDGVGGLQLSLFDETIEHLLFEPTFIIDYPA
                     EVSPLARRNDANPAITDRFELYIAGREIANGFSELNDPEDQAARFLEQAKAKDAGDNE
                     AMHYDGDYIRALEYGLPPTAGEGIGIDRLIMLFTDSPSIRDVILFPQLRKED"
     gene            3817..4503
                     /locus_tag="PKZ49_02725"
     CDS             3817..4503
                     /locus_tag="PKZ49_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35504.1"
                     /translation="MLSVQTENKHLLIWVVTVLIFFMIKEVSAQNYPPPEIIQGSRHA
                     INEQTNPKTQLADNPATQVLPPQPKTRSAVTYSTRSVLQKDSTDTSAHNSVRIRSVEA
                     GRIVRPYGQPACVNCGVINYVYPSGHARSGGGYGNAMAGIPGGVVAIPLGEDGEGRFI
                     APIGSILGNTSPYPNQISQGQTGGSVMYYDIGVMLENGTQTVVRQETKPHFNIGERVK
                     LIDGVLLPSP"
     gene            complement(4507..5685)
                     /locus_tag="PKZ49_02730"
     CDS             complement(4507..5685)
                     /locus_tag="PKZ49_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF839 domain-containing protein"
                     /protein_id="HND35505.1"
                     /translation="MKAHSFNTQRRQLLRLSGLVLGTLLTQSFWTSRNSWASSTPLSS
                     VGPLQLSDKNGVRLPKGFTSRIVAASGQNLFGYRWHSAPDGGATFTTEDGGWIYVSNS
                     EIDKKMGGTGALHFNSEGTVINAYSILKNTSRNCSGGTTPWQTWLSCEEIERGYVWEC
                     DPFGKNKAQVRPELGCFRHEAIAFDVNSNKLYLTEDQPDGCLYRFTPYEMMDSGHVSL
                     DEGFLEVAEVIVSGHAKIIRWHRLPDPSAAAIETRKQVKHCTQFNGGEGIWYYGGKVY
                     FTTKGDNRVWVYNTQNHSLHIIYDASHYTSPVLIGVDDITSNELGELLVAEDKGNMQI
                     VILSGSTIKPLLQLVGHDQSEITGLAFSPDGSRLYFSSQRGKTGRNENGVTFEISGFR
                     "
     gene            complement(5697..6374)
                     /gene="pgl"
                     /locus_tag="PKZ49_02735"
     CDS             complement(5697..6374)
                     /gene="pgl"
                     /locus_tag="PKZ49_02735"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HND35506.1"
                     /translation="MLERRHYENINTLSTAVADFAADRLHTALQQNDVASLVIPGGST
                     PKFYLPTLARLPINWQKVMITLSDERWVDTRSQDSNEFLIRNCFLNCMENPARFIGLK
                     TTHDHPSLALLTINDRLSQLPLPINLAILGLGEDGHIASIFPGHSINSASNSLCEVAV
                     PPIAPSIRISLAFSLLASSDHIIVVVTGQNKRKRLEQFIDSQDPQIPFIRLVQQTHSP
                     ITIFESN"
     gene            complement(6368..7012)
                     /locus_tag="PKZ49_02740"
     CDS             complement(6368..7012)
                     /locus_tag="PKZ49_02740"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HND35507.1"
                     /translation="MLFLNWQAIIFDFDGVIVESGDIKTQAFGELYRSYGPEVEAAVM
                     EYHKQNGGMSRYRKFHYFQEQLLHQPPLTPQEEQDLDQRFSELVIEAVINSESVPGAE
                     ALIHNAYKHIPLFIASGTPEKELQRIVEQRGLASYFIDVRGSPQTKQALIAEILGAHA
                     LTPEKVLMVGDAMADYLGAKANNTAFLGRVRPGDTNPFPADVKVVSDLHPLLTC"
     gene            complement(7016..7960)
                     /locus_tag="PKZ49_02745"
     CDS             complement(7016..7960)
                     /locus_tag="PKZ49_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HND35508.1"
                     /translation="MNNASVSDKMVVVFGASGFLGSHVADALSGAGYQVRLFDHISSP
                     YQRIDQEMIVGDIMDIDQVIEATRGAHAVYNFAAIADIDEAHNKPVATATINVLGNMH
                     VLEAARIAGANRFVFASSVYVYSESGSFYRASKQAAERFTETYHDRYGLDYTILRYGS
                     LYGRRADLRNGIYRMLHEAIQKHSITYHGSGNAMREYIHVEDAARMSVQVLSPEFANR
                     HLILTGQERMRIRDVMTMISEIMPWNVELHFEEANAISHYEITPYAFQPRIGRKLVLN
                     EHVDIGQGILDCIREIHQKICHTEDEDNAFPSTSDNKA"
     gene            complement(7953..8888)
                     /locus_tag="PKZ49_02750"
     CDS             complement(7953..8888)
                     /locus_tag="PKZ49_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HND35509.1"
                     /translation="MSKIVISTSSFDIQNNPYIERLTKRGMQIVLNPYQRKLSEDEIL
                     TLLGNDTIALLAGIEPLTKKVLTAARGLKVIARCGTGLDSVDLEAAHQCNILVSNTPE
                     APAQAVSELTMGLILSVLRQICSTHRLIQDGQWPRTQGHLLAAQTVGIIGLGHIGRRV
                     ATLCLAFGARVIAHDPYLQQAHPDIPLVNFEQLIKEANIISLHIPYSQGTHHLIDADT
                     LSRMKPGTVLINAARGGLIDENALVEALTSGHIAGAALDVFEQEPYQGPLLNCSNVVM
                     TSHMGSLAKESRQRMEHEAAENLLNGLITAGLLNE"
     gene            complement(8885..9646)
                     /locus_tag="PKZ49_02755"
     CDS             complement(8885..9646)
                     /locus_tag="PKZ49_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HND35510.1"
                     /translation="MSLKERLARSELTIGSWITLGHPAIAEIMASAGFDWLVLDMEHS
                     VMELSEAQIIIQVLDKQQCPAIVRLTSNHPDQIKRVMDAGATGVMIPMIKSAEDAKAA
                     VSSVYYPPQGQRGVGLARAQGYGARFQEYRQWLIENAVIVVMIEHIKAVEQIDSILAV
                     PGVDAYIIGPYDLSGSMGMPGELDHPQVQDAIKHVLDAGKRLQKPGGIHVIEPDSAAL
                     QQRIRSGFSFLGYSLDIRILDTICRTHLQSIKKQL"
     gene            complement(9661..10410)
                     /locus_tag="PKZ49_02760"
     CDS             complement(9661..10410)
                     /locus_tag="PKZ49_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HND35511.1"
                     /translation="MKIIAIIPARMGSTRFPGKPLANLLGRTMIEHVYKRVALSKFIS
                     ATYIATCDEEIRQAATAFGAPVIMTADTHERASDRIAEAVANIKGDLIVMVQGDEPMT
                     HPEMIDTAVRPFENDSQLGCVNLVRRIEQEADYHDVNTIKVVMNQQGDALYMSRQPIP
                     TLSKAGFSATQAYKQVCIIPFRRDVLLRYTQLAPTPLEKLESIDMLRLLEHGYPVRMI
                     ETQYNSQAVDTEADLARVAQLMATDPLLSQY"
     gene            10660..11940
                     /locus_tag="PKZ49_02765"
     CDS             10660..11940
                     /locus_tag="PKZ49_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35512.1"
                     /translation="MKALFFLRHYNDIDHITPIIAKWVECGHHCDVVLIGASRFRKDF
                     RIAYLSKLPGTRVNHIREVFSAWLFLKWRLQMLLLTGNLRRLFIGPFVAWLAKRYDAD
                     RRLPLWQETTNNLLSRCFDQTNQGIVAFDWIERNSVIAVEWVETVIGMARARGLGTVS
                     LPHGDSPHANQLIRRGEWKVGPDPVFSTARIFDRLAVPNELCAKRFEPFLPKQAIAVL
                     GSPRYCEEWLDKLSQLMPPSSLEHAEHKLKIVMFLRKANFTTFWEEVSEVVHLLATFP
                     NVELIIKPHTRSGWKQSLTRSKSIKQLANVKIADDQMHSIQLMNWADVCIDLATSVVF
                     EAVRLKKPVLAADYLHAGRSAIAVYMPETELRCRDDVYEKIQRFLTEGTEDFYIESHR
                     QHFLDQMLDVGGKDVLAHYIALLESTVKLNEKKQ"
     gene            11975..12742
                     /locus_tag="PKZ49_02770"
     CDS             11975..12742
                     /locus_tag="PKZ49_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HND35513.1"
                     /translation="MLDFLKKLVVMAYMLKGGQTKVTVRNQPLVISVDHWRVLGRAKN
                     YSIKEPDTLDWLDGFEPGTCYFDIGANIGQFSLYPAKKYGQKVEIYAFEPQSNNYYAL
                     NKNIYLNQLKDSITAYCVAISGESKFDKLYVPKFIPGGNRSQFGEESQAAMGVPTSHI
                     QGMFGITLDDLCEKWNFPYPHYMKIDVDGIEISILRSASKVLKHPNLKSVIVELGTPL
                     EQQEAIAIMKQAGLELKHQSTRNWGETCFIFERTSTA"
     gene            12775..14040
                     /locus_tag="PKZ49_02775"
     CDS             12775..14040
                     /locus_tag="PKZ49_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35514.1"
                     /translation="MKALFFLRHYNDIDHMTPVIAKWIETGHICDVVMISKKRFDGDY
                     RIEYLKHLQGVRLAHIRDLLPVFDFFMWRLQTLLLVRSSYRSFLKPLVMFLATIFRQE
                     KRKKVWEKVSRYLLKRSFNDLNNGVVAFDWIERNSETAVEWVDVFVNLARNQGLGVIS
                     LPHGDSPHASQLIRHGEWLLKPDVSFSAAGMFDRVVVPNALCAIRFRPFLDDRKLVVL
                     GSPRYCEEWLTKLKSLLPESPLQNSRSRLKIVLFLRKANFTTFWEELSEVVQMLSTFE
                     EVELAIKPHTRGGWKQSFTKSAAIQHLKNVKIIESDIHSIHLIHWADVVIDLATSIAY
                     EAIRAKKPLLAADYLHAGRSAAAQYMPETELRCRDDVYERIHHFLQNGCENYYIEGNR
                     QKFITEMLESPGTDVLPHYVGLLEALASK"
     gene            14102..15340
                     /locus_tag="PKZ49_02780"
     CDS             14102..15340
                     /locus_tag="PKZ49_02780"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HND35515.1"
                     /translation="MSEKQISMTYLLSERLISVEWRERFIRFSLIFFCFALWQKWSFF
                     YAFFLLSGAWILDGGLRNFKQLIREPLVQGILVFCSVVAIGLLWGDYIETNQNKWKKY
                     FILLTFIPFFALLNKDSARFPWVSNTFLIVYGSVLLVGAYQHFVQGLIGVPALDISYL
                     SFSAILGVGVITTIFLAQVSQTILQNMIWWFISALMLLLQFNQGARTVLFATMVAAII
                     LIFFMGQKKRKMRLIGISSFIGLALLLMATNSVMQERLSLVSHDWAAIKLGNYETSLG
                     YRLAIWDIGLNGIQEQPWIGHGTGNPEVYFEKETQTYKDGIYRNLPAFQKTSHYHNDW
                     IEVGMHWGGLGIMALAFLIFSWLRIFKENQLANLGIAFVSYIVLAGVTDTFLLYNRVP
                     PILLVITALLVCWQRSKMKS"
     gene            15396..16076
                     /locus_tag="PKZ49_02785"
     CDS             15396..16076
                     /locus_tag="PKZ49_02785"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HND35516.1"
                     /translation="MKRFEPLQSEHLVDLIPVNPAIECYMQGLIERRTDSVLVEMEAL
                     AKQKNFPIVGRLVGTSLEVLAKMINAKRVFEFGSGYGYSAYWFARAVGLNGQVICTDN
                     KLANKEQAEIFLSSVDLWKRVTFYIGYAQAVFSQTEGFFDICYNDVDKSEYPSVWKMA
                     REKIKPGGLYIADNVLWHGKVIAENNTDEWTQAILEHNQLIFDDPEFDAFINPTRDGV
                     MVARRKMA"
     gene            16152..16409
                     /locus_tag="PKZ49_02790"
     CDS             16152..16409
                     /locus_tag="PKZ49_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2024 family protein"
                     /protein_id="HND35517.1"
                     /translation="MQIHVYDTYVKAKDGHIMHFDVFTSVRDDQKAIGYAKQWLTSIG
                     EEDAIVSSKECRFCHSQGAPDDVIEAINKDGYFIYKMEGCR"
     gene            16453..16968
                     /locus_tag="PKZ49_02795"
     CDS             16453..16968
                     /locus_tag="PKZ49_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HND35518.1"
                     /translation="MKQVPFKRSLFIALFLACGLSHAADPKMIGEFNDWTAYVYMEGK
                     NKVCYMVSKPKKEEGSYTKRGSVYALLTHRPAENSKNVFSFMAGYPFKKESEVTVSIG
                     NQNFKLFTDNETAWAQDSDTDNKIANAIRNGSALIIKGVSARDTATTDTFGLKGSSSA
                     YGAISKECGIK"
     gene            17016..17363
                     /locus_tag="PKZ49_02800"
     CDS             17016..17363
                     /locus_tag="PKZ49_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HND35519.1"
                     /translation="MDEVITIYQKPTCSKCRATLELLTQSGEEFQSINYYEDPISEDL
                     LRELLQKLNMTAREIFRSDEPAARGLQSATDEELLKRMAANPDLIQRPIVVRGEKAIL
                     ARPPEQVLQLLKK"
     gene            17603..18037
                     /gene="rplM"
                     /locus_tag="PKZ49_02805"
     CDS             17603..18037
                     /gene="rplM"
                     /locus_tag="PKZ49_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HND35520.1"
                     /translation="MKTFSAKPLEVQHDWFLIDATDKVLGRLATAIAHRLRGKHKPIF
                     TPHVDTGDYIVVINVDKIKVTGNKAEDKKYYRHSGYPGGIYETTFKKMHARFPARPLE
                     KAVKGMLPKGPLGYAMIKKLKIYAGDTHPHSAQQPKNLEIKA"
     gene            18041..18433
                     /gene="rpsI"
                     /locus_tag="PKZ49_02810"
     CDS             18041..18433
                     /gene="rpsI"
                     /locus_tag="PKZ49_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HND35521.1"
                     /translation="MAINYNYGTGRRKSSVARVFIKPGKGAFVVNDKPLDEYFSRETG
                     RMVVRQPLELTGNTANFDIMVNIHGGGESGQAGAIRHGITRALIDYDETLKPVLSKAG
                     FVTRDAREVERKKVGLRKARRRKQFSKR"
     gene            18513..19550
                     /gene="argC"
                     /locus_tag="PKZ49_02815"
     CDS             18513..19550
                     /gene="argC"
                     /locus_tag="PKZ49_02815"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HND35522.1"
                     /translation="MIKVGIVGGTGYTGVELLRILAQHPEVSLKVITSRQEAGLAVDE
                     LFPSLRKYVSLKFVDPEKAKLDKCDIVFFATPNGVAMRQARELVDAGVKVIDLSADFR
                     IKDISEWEKWYGMSHACPELVSEAVYGLPEVNRKQIKKARIIANPGCYPTAVQLGFLP
                     LIEAKVIDTNSLIADTKSGVSGAGRKAEVHTLFPEAADNFKSYGVPGHRHLPEIRQGL
                     SNVAGKLVGLTFVPHLTPMVRGIHATLYAKLMSIKTKVDLQTLFEERYKNEHFVDVLP
                     AGSHPETRSVRGSNYCRIAVHRPQGGQTVVVLAVTDNLVKGAAGQAVQNMNLMFGFSE
                     TLGIEHVPILP"
     gene            19616..19981
                     /gene="erpA"
                     /locus_tag="PKZ49_02820"
     CDS             19616..19981
                     /gene="erpA"
                     /locus_tag="PKZ49_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HND35523.1"
                     /translation="MQLNSLEENQAIQGQLVFTDSAASKVKQLIEEEGNPNLKLRVFV
                     SGGGCSGFQYGFTFDELENEDDLVIEKEKVKLLIDSMSYQYLIGAEIDYVENSQGAQF
                     VIKNPSAASTCGCGSSFSV"
     gene            20028..20516
                     /locus_tag="PKZ49_02825"
     CDS             20028..20516
                     /locus_tag="PKZ49_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HND35524.1"
                     /translation="MPSFDIVSEVDKTEVKNAIDQTNKEVGTRFDFKGSDARVEQAEM
                     VLTIYADNDFQLSQVQDILNTKLSKRGVDMRCLDVSDKIEKVSGNKVKRSCTVKVGIE
                     TELAKKIIKLIKDSKIKVQASIQADTVRVSGKNRDDLQQCIAFVKKTIAEFPLQYQNF
                     RD"
     gene            complement(20531..>20924)
                     /locus_tag="PKZ49_02830"
     CDS             complement(20531..>20924)
                     /locus_tag="PKZ49_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HND35525.1"
                     /translation="NWKEAVDAGQNPRDIKVTFAQEIVTRFHSKAAAEHALIDFETRF
                     KNGLLPDDIPQKTIQINGNEVTISQLLKQVDLTSSTSEAFRMIEQGGVKIDGDRISDK
                     SLKIASGKTITIQVGKRKFARVTINSAK"
BASE COUNT         5985 a         3989 c         4757 g         6193 t
ORIGIN      
        1 aatgggcgtc gtaggaactg ggaatgttgc gatgtcaagc gccccagtaa acaggcggcc
       61 tgccatatga attataccga tgaaaagtcc tgcggtcatt ccataggttg ctccttcttt
      121 acgtcctgtg atcatcatcg ttttaggcac ttccgctaca cccgtcacca catttgcgat
      181 tccggtacct aatttttcac ctactttggt tgggtaatct ttggccatac tggtggtagg
      241 aagtagaaaa aagatcacga gtagcaagaa gagttttgat gcaaaaattt ttttcatgtt
      301 atttttttcc caaaaaaacg gaatcataca ctattgaatt atcgattggc ttcgatgatt
      361 gaagattatc cttgacctgc aatttttccc caagtgtcgc gtaacgttac ggctcgattg
      421 aatacgggtt ttccaggcga agtatctacg cggtccgcaa taaaatagcc gtgtcgctca
      481 aattggaaaa tttgctcagg ctgtacttca aacaatgacg gttcaagata agccatggca
      541 atttttttgg aatcagggtt gaggtaagtt ttaaaatcct gttcacctgc atcaggatag
      601 ggttgggtaa ataaccgatc atatagcctt atttctgcag aacgagcgta tttactggaa
      661 agccaatgga tatttccttt aactttacgc gcatcagcgc cgggtgttcc gcttttggta
      721 tcgggatcat aggtgcaatg gattgcttcg atttgtccgc ggtcgttctt gataatagaa
      781 gtgcatttta cgatataggc gtagcgtagg cgtacctcgg ccccgggaga taagcggaaa
      841 tagcctttgc ttggattctc catgaaatca tcttgatcaa tgtaaagcgt tttggtgaaa
      901 gaaagcgtac gtttacctaa ttcaggccgt tgtgggtgat taggcgcata gcattcttct
      961 tcttggtttt ctgggtagtt gtcaataatc aaacgaagtg gattcagtac ccctattctc
     1021 cgtaaagcgc gctcgttcaa atcttctcgt aaacaatcct cgagcacggt catatcgatc
     1081 caagaatctg ctttgctgat cccgatccgc tcggtaaaga gtcgtattgc ttccggcgta
     1141 tagccacgcc gacgaacgcc tgctagtgta ttcattcttg ggtcatccca accatcgact
     1201 agcttattct caacaagctc gatcagctta cgtttgctca tcacactgta ggttagattt
     1261 aatcgagcaa attcgatttg ttgtgggtga caaggaacct tgtcttgcaa ctgatcgagg
     1321 acccaatcat acaatggacg atggtcttca aattccagtg tgcaaagtga atgcgtgatc
     1381 ttttctaagg cgtccgaaat acaatgggta aaatcataca tggggtaaat acaccattta
     1441 tcaccggtac gctgatgatg gatatggcga atacggtaaa taactggatc ccgcaaattg
     1501 atattgggag aactcatatc aatttttgct cgcaatacat gactgccatc tggaaattca
     1561 cccgcacgca tgcgccggaa taagtctaga ttttctttga ttgtgcgatc acggtacggg
     1621 ctattgatcc ctggttggtt taatgtgccc cttaattgcc gaatttcctc ggcagatgag
     1681 ctatcaacat aggctttgcc gttttcgata agaaattcag cgaatgcata aagctgctca
     1741 aaataatctg aggcatagta aatatgtttt ccccagtcaa aaccaagcca tttgacagaa
     1801 tcacagattg cgtcaacata ttcctgcgct tctttttctg gattggtgtc gtcaaaccgg
     1861 agatggcaaa atcccgcata gtccttagcc aatccaaaat ttaggcaaat actttttgca
     1921 tggccaatat gcaagtagcc atttggctct ggggggaagc gagtttcaac ccgctgattc
     1981 catttgcctg aacggttatc gtcttcgatg atgttacgga taaaatttga agctgtagaa
     2041 gatgtcgtca caaaaattct ttcgattgat tagattgaga atgtagttat cagcgaacta
     2101 tatataatat ggtattaatc gctttatatt atagtgcgtg attcgtttga taaaacgaga
     2161 ataagcaaat agttgggcat ttgttttctg tacctagcta attatccgta attcctacct
     2221 aatctaatca tatgaccgaa gaatccgagt taactccctc ccaagatgaa aaccatatta
     2281 tcgctgagcg acgcgccaaa cttgctgagc taaggaagcg agccaatcca tttccaaacc
     2341 attttaggcg tgatagcttt gctatgtcgc ttcaggatac ttactgtaat cagacgaaag
     2401 aagatttgga aggacagcaa atttccgtga aagttgctgg acgaatgatg tttaagcgcc
     2461 ttatgggtaa agccagtttt gcaacgattc aggatatgag tggcaaaatt cagctctatg
     2521 tgtccaatga tcatactggg gaggctgcgc atgaagcatt taagcactat gatcttggag
     2581 acattctcgg tgcgcaaggc actttattca gaaccaagac aggcgaactt tcagtgcgtg
     2641 tcacgcattt acagttatta acgaagtcat tgcgaccctt acctgaaaaa tttcatggac
     2701 ttacggacca ggaacaaaaa tatcgtcaac gctacctgga tttaattacc aatgagaata
     2761 cacgcaaggt atttatcgtt cgttcgaaag tgatccaagc aattcgggaa tttttggttg
     2821 ctagagatta tcttgaagtg gaaacaccaa tgatgcatcc aattccagga ggagcaactg
     2881 cgcgaccgtt ctcgactcat cataatgcac tcgatatgcc cctgtatctg cggattgctc
     2941 ctgaattata tttgaaacga ttagtcgtcg gtggaatgga gaaagtgttt gaaattaata
     3001 gaaacttcag gaatgaaggg atatctactc gacacaatcc agagtttaca atgctggaat
     3061 tttacgaagc ataccaagac tatacctatc taatggactt gacagaatcc atgctgcgtg
     3121 aagtggcgcg ggctgtaatg ggaacgacac atattaccta tcaagaacaa gaaatggacc
     3181 ttgcaaaacc ttttgcaaaa ctcaccatta cccaagcgat tcaaaagtat tttcccgagt
     3241 attcgacggc tcaacttaat gatcgagagt tcttgattag tcggttacaa gcactcaata
     3301 tagcgtacaa acctgaagat ggggtgggtg gcctccaact ttcattgttt gatgaaacga
     3361 ttgaacattt gttatttgaa ccgactttta ttattgatta tcctgcagaa gtatcacccc
     3421 ttgctcgtcg gaacgacgca aatccagcaa ttactgatcg ttttgaattg tatattgcgg
     3481 gacgagagat cgcgaatgga ttctcagaat tgaacgatcc agaggatcag gctgcacggt
     3541 tccttgaaca agctaaagcc aaagatgccg gtgataatga agccatgcac tatgatggag
     3601 attatattcg ggcgcttgaa tatggtttac caccaacagc aggcgaggga atcggaatcg
     3661 atcgattaat catgcttttc acggatagtc cgagcatacg tgacgtcatt ttatttccgc
     3721 aactccgtaa ggaggattga acttattccc atttaacaag gccttatttc agtcgatgat
     3781 atagtaacaa ttgatactag ttcggagaaa ctgataatgt tatctgttca aactgaaaac
     3841 aaacatttat tgatatgggt agtcactgtt cttatttttt tcatgataaa agaagtgagc
     3901 gcacagaatt atccacctcc tgaaataata caaggatcac ggcatgcaat aaacgagcag
     3961 actaacccca agacccagct cgctgataat cctgcaacgc aagtattacc acctcagcct
     4021 aaaacgagat ccgcagttac ttattctact cgaagtgtgc tccagaaaga ctcaaccgat
     4081 acctcggcac acaatagtgt acgtattcga agtgtcgaag cagggagaat tgtaaggcct
     4141 tatggtcaac ctgcttgtgt gaattgtggt gtgattaatt atgtttatcc ttctggccac
     4201 gcaaggagtg ggggtggata tgggaatgca atggcaggaa ttcctggcgg tgttgttgcc
     4261 attcctttgg gtgaagatgg ggaaggtcgg tttatcgcac cgattggtag tatattagga
     4321 aatacttcgc cgtatcctaa tcaaattagc cagggtcaga ctggtggtag cgttatgtac
     4381 tacgatatcg gggtgatgtt ggaaaatgga acgcaaaccg tagttaggca agagactaag
     4441 ccgcacttta atataggtga gcgagtcaaa ctgatcgatg gcgttctatt gccaagccct
     4501 taattgttat cggaatccgc tgatctcaaa cgtaacacca ttttcattcc gccccgtttt
     4561 tcctcgctgt gaactaaaat agagtcgaga gccgtcaggg ctgaatgcaa gtcctgtaat
     4621 ttcagactga tcatgaccaa cgagctgtaa caatggctta atcgtgcttc ctgacaaaat
     4681 gacaatttgc atattgcctt tgtcttcggc cacgaggagt tctcctagtt cattggaagt
     4741 aatatcatca acacctatca atacagggga agtatagtgt gatgcatcat aaatgatatg
     4801 gagtgaatgg ttttgcgtgt tatagaccca aactcgatta tctcctttag tagtgaagta
     4861 tacttttcct ccatagtacc aaataccttc tccaccgttg aactgcgtac agtgtttgac
     4921 ttgtttgcgt gtttcaattg cagctgcaga tggatcaggg agtcgatgcc aacgaattat
     4981 ttttgcatga cccgatacga taacttctgc tacttccaga aatccttcat ctaaggatac
     5041 gtgacctgaa tccatcattt cataaggggt gaaacgatat aggcagccat cgggctgatc
     5101 ttcggttaga tagagtttat tactgttgac atcgaaagcg atggcttcgt ggcggaaaca
     5161 accgagttcg ggtctcacct gggctttgtt ttttccaaat ggatcacatt cccatacata
     5221 gccacgttca atttcttcac acgataacca agtttgccat ggcgtggtgc cgcctgagca
     5281 atttcgtgag gtgtttttta aaatagaata ggcattgatc accgttcctt cagaattaaa
     5341 gtgaagtgca ccagtacccc ccatcttttt atcgatttca ctatttgaaa cataaatcca
     5401 gcccccatcc tcagtcgtaa acgttgctcc accgtcaggt gctgaatgcc atcgatagcc
     5461 aaaaagattt tgccctgaag cagcaacgat tcttgaggta aatcccttag gtaagcgaac
     5521 tccattttta tcggaaagtt ggagggggcc tactgatgaa agtggtgtgg aacttgccca
     5581 gctatttcga gatgtccaga aggattgggt aagcagtgta ccaagcacta aaccgcttaa
     5641 ccttaataac tgacggcgct gggtattgaa cgaatgagct ttcataagtt ttcaggctaa
     5701 ttggactcaa aaattgtaat aggggagtga gtctgttgaa caagcctgat gaaaggtatc
     5761 tggggatcct gactatcgat gaattgctca agacgtttcc ttttattttg accggtaacg
     5821 acaacgataa tgtgatcact ggaagcgagc agcgaaaatg ccaagctgat gcgaattgag
     5881 ggcgctattg gtggaactgc tacttcgcat aatgaattac tcgctgagtt tatagaatga
     5941 ccgggaaaaa tagaagcgat gtgaccatct tcccctaagc ctaatatcgc taagttaatc
     6001 ggtagaggca attgtgaaag ccgatcgttg attgtcaata aagccagaga aggatgatca
     6061 tgagtggttt ttaaaccgat gaagcgtgct ggattctcca tgcaatttaa gaagcaattt
     6121 ctgatcagaa actcgtttga atcttgtgaa cgggtatcca cccagcgttc gtcactcagc
     6181 gtgatcatca ccttttgcca atttatgggc aagcgtgcta atgttggcaa gtagaatttg
     6241 ggtgtgcttc caccgggaat cactaaactc gcaacatcgt tttgttgcaa agctgtatgt
     6301 aaacgatctg ctgcaaaatc agcgacagcg gttgaaagtg tatttatatt ttcatagtgc
     6361 cgacgctcta gcatgttaat aaggggtgta gatcagatac aactttgaca tcggctggga
     6421 aaggattggt atcgccaggc cggactcgac ctagaaaagc ggtattgttg gcttttgcac
     6481 caagataatc ggccattgca tcaccaacca tcaggacttt ctcaggagtc agcgcatgtg
     6541 cacctagaat ctcagcaatc agtgcttgtt tggtttgtgg tgagccacgt acatcgatga
     6601 aataagatgc cagtccgcgt tgttcgacaa tgcgctgtag ttctttctca ggtgtgcctg
     6661 aagcaataaa caggggaata tgcttataag cattgtgaat gagcgcttca gcacccggaa
     6721 ccgattcgct gttaatgact gcttcaatga ccaattcaga aaatcgttga tccaaatctt
     6781 gctcttcttg gggtgttagc ggtggttgat gtaggagctg ttcttgaaaa taatgaaatt
     6841 ttcgatagcg agacattccg ccattttgtt tatgatattc catcacggct gcttcaactt
     6901 ccggtccata ggagcggtat agctcaccaa atgcctgtgt tttgatatca ccggattcaa
     6961 cgatgacgcc atcaaaatca aagatgatcg cttgccaatt caaaaatagc atctattagg
     7021 ctttattatc tgaagtggaa gggaatgcgt tatcttcatc ttccgtatgg cagatttttt
     7081 ggtgaatttc cctgatacag tcaagtattc cctgcccaat gtcaacgtgc tcatttagga
     7141 ctagttttct tccgattcga ggttggaaag catagggtgt aatttcataa tgcgaaatcg
     7201 cattggcttc ttcaaagtgc agttcaacat tccatggcat gatttcagaa atcattgtca
     7261 ttacatcgcg tatccgcatt ctttcttgtc ccgttaaaat cagatgtcgg ttagcaaatt
     7321 cgggggagag aacctgaaca ctcatccgtg ccgcgtcttc aacatgaata tactctcgca
     7381 tggcatttcc gctaccatga taagtgatac tatgtttttg tatcgcttcg tgtaacattc
     7441 ggtaaattcc attgcgcaga tcggcccgtc gaccatataa agagccgtag cgtaatatcg
     7501 tataatctag gccatatcga tcgtggtaag tttcagtaaa acgttcagca gcttgcttgc
     7561 tggctcgata aaacgaccct gactctgaat agacataaac actactagcg aaaacaaagc
     7621 gatttgctcc cgcgattctc gctgcttcta ggacgtgcat gttacccaat acattgatgg
     7681 tagctgttgc aactggttta ttatgagctt catcaatgtc cgcgattgca gcaaaattat
     7741 agacagcatg agcgccacgc gttgcttcaa taacctgatc gatatccatg atatcgccta
     7801 cgatcatctc ttgatcgatg cgctggtagg gagaagaaat atgatcgaac aatcgaactt
     7861 gataacccgc tcctgacaat gcatctgcca catgactgcc taggaagccg ctagcaccga
     7921 ataccacgac cattttgtct gatacagaag cgttattcat tgagtagacc tgctgtgatt
     7981 aatccattca ataaattctc agctgcctca tgttccattc gctgacgaga ttctttagcc
     8041 agtgatccca tatgagaagt catgacaaca ttactgcagt taagcaacgg tccttggtaa
     8101 ggttcttgct caaatacatc caacgctgct ccagcaatat ggccagaagt aagtgcttcc
     8161 acaagtgcat tttcgtcaat caagcctccc cgtgcagcgt tgattagaac cgtaccgggc
     8221 ttcatacgac ttagggtatc tgcatcaatg agatgatgcg taccttgaga ataagggata
     8281 tgaagtgaaa tgatattcgc ttccttgatg agttgctcaa agttgactaa tggaatatca
     8341 ggatgtgctt gctgtaggta aggatcatgt gcgatgactc tggccccaaa agcaaggcac
     8401 aatgttgcaa cgcgtcgacc gatatggccc agcccaataa tcccaaccgt ttgagcggca
     8461 agaagatgtc cctgcgttct tggccattgg ccatcctgaa ttaatcggtg agtggagcaa
     8521 atttgtcgca acacagaaag gattagtccc atcgttaatt ctgaaactgc ctgagcaggg
     8581 gcttcaggtg tattggaaac taggatgtta cactggtgag cagcttctaa gtcgacgcta
     8641 tctaaaccag taccacaacg ggcgataact ttgaggcctc gagcagcagt caaaactttc
     8701 ttagtgagtg gctcaatacc agccagaagg gcgattgtat cgttgccaag taaagtgaga
     8761 atttcgtctt cactgagctt gcgttggtag ggattgagta caatctgcat tcccctcttg
     8821 gttaatcgtt cgatgtaagg gttattctgg atatcaaacg atgaagtaga tataacgatt
     8881 ttactcataa ttgttttttt atcgattgta ggtgcgtgcg acagatggta tctaaaattc
     8941 gtatgtctag actatagccc aagaaactaa agcccgaacg aatgcgctgc tgtaaagctg
     9001 ctgaatccgg ttcgatgaca tgaataccgc caggtttttg taatcgctta cccgcatcta
     9061 aaacgtgttt gattgcgtct tgcacttggg ggtgatctag ctccccaggc atgcccattg
     9121 aaccggaaag atcataaggt ccaataatat aggcgtctac accaggaacc gctaaaattg
     9181 aatcaatctg ctcaaccgct ttgatatgtt caatcatcac cacgatgact gcattttcga
     9241 ttagccattg acggtactcc tggaagcggg caccatagcc ctgggcgcgt gctaatccaa
     9301 caccacgctg tccttgcggg ggataataga ccgagctcac tgcggccttg gcgtcttcag
     9361 cagacttgat catggggatc atgactccag ttgcgccagc atccatgacg cgtttgattt
     9421 gatccgggtg attggatgtc agtcgaacga ttgcagggca ctgctgtttg tctaatactt
     9481 gaatgatgat ttgagcttcg ctgagttcca ttacactatg ttccatgtcc agaaccagcc
     9541 aatcaaatcc agccgatgcc atgatttcgg caatagcagg atgccctaat gtgatccagg
     9601 aaccaatcgt caattcagag cgagcaagcc tttcttttag cgacatcgtg tagtcctata
     9661 tcaatattga gacagtaagg gatcagttgc catcaattga gcaacacgag ccaaatcagc
     9721 ttcggtatca actgcttggg agttgtattg ggtttctatc attctgactg ggtagccatg
     9781 ttcgagtaat cgaagcatat cgatggattc cagtttttcc agcggtgtag gcgctagctg
     9841 cgtataccgt agcagtacat cgcgacgaaa gggaatgata caaacttgct tataagcctg
     9901 ggtcgcagag aatccagctt tagataacgt tggaataggt tggcgcgaca tatagagtgc
     9961 atccccttgc tgattcatga cgactttaat cgtattgaca tcatggtaat cagcttcttg
    10021 ttcgatacga cgcaccaagt taacacaccc gagttggcta tcgttttcaa aaggtctgac
    10081 cgcagtatcg atcatttctg gatgtgtcat cggctcatcg ccttggacca tcacgattaa
    10141 atcccccttg atattcgcaa cggcttctgc aatccgatca ctcgctcgtt catgggtgtc
    10201 ggccgtcatg ataacaggtg ctccaaatgc ggtagcagct tggcgaattt cttcatcaca
    10261 ggttgcaata taagttgcag aaataaattt acttaaggcg acccgcttgt aaacatgctc
    10321 tatcattgtt ctaccgagta aattggccag tggtttaccg ggaaaacgtg tcgatcccat
    10381 gcgagcggga ataattgcaa ttattttcat tagtcgatat aaatttttag ggtcaaatta
    10441 ctaacaataa caggaaaaag cgtttgtagt gatgggttgt attctaaaag gaagtttaag
    10501 tctgtatgtt cctgaagatg ataatccgcg aattgattat gttttttggt aaataaaatc
    10561 gcgcacgtgt aaaattacaa attcattatt tcaattatca cagcaacacc taaacttttt
    10621 tcctacgctt tacgcatcca ctacaatcat aacataggta tgaaagcgct cttttttctt
    10681 cgtcattata atgatatcga tcacattaca ccgattatcg ccaagtgggt cgaatgtggg
    10741 catcattgtg atgtggtgct gattggtgcg agtcgttttc gaaaagattt tcggattgcc
    10801 tatctaagta agctgccagg aacaagggtt aatcatattc gtgaggtttt ttcggcttgg
    10861 ttattcttga agtggcgttt gcaaatgttg ttgttgaccg gtaatttacg tcgtttattc
    10921 ataggtcctt ttgttgcatg gcttgcaaag cgttatgatg cagatcgaag gctgccattg
    10981 tggcaggaaa ctacaaacaa tttattatcg cgatgctttg atcaaacaaa ccaaggtatc
    11041 gtcgcttttg actggataga aagaaattct gtgattgcag tcgaatgggt cgagacagtg
    11101 attggcatgg cacgtgccag ggggctgggt actgtttcat tgccccatgg cgatagcccc
    11161 catgcgaacc agcttatccg cagaggtgag tggaaagtgg gtcctgaccc cgtgttttcc
    11221 acggccagaa ttttcgaccg actcgctgtg cccaatgagt tatgcgcaaa acgatttgag
    11281 ccgtttttgc cgaaacaagc gattgctgtc ttgggttctc cgcgttattg tgaggaatgg
    11341 cttgataaat taagccaatt gatgccccct tcgtcgctgg aacatgcgga gcataaactc
    11401 aagattgtga tgtttttgag gaaagccaat ttcacgacgt tctgggaaga agtaagtgaa
    11461 gtggttcatt tgctagcaac atttcctaac gtagaattaa ttatcaagcc gcatacccgc
    11521 agcggctgga aacaatcgct cacccgaagt aagtcgataa aacagttagc caatgtcaaa
    11581 atagcggatg atcagatgca ttctatccaa ctgatgaatt gggcagacgt ttgtatcgat
    11641 ttagcgacct ccgtcgtatt cgaggcagta cgactcaaaa aaccagttct cgctgctgat
    11701 tatctgcatg cggggcgttc tgctatcgca gtttatatgc cagaaacgga attgcgttgc
    11761 cgagatgatg tttatgaaaa aattcagcga tttcttaccg aaggaactga ggatttttat
    11821 atcgaaagcc atcgccaaca ttttttagat caaatgctag acgttggggg aaaggatgta
    11881 ttggcacact atattgcgct acttgaatcg acagtaaaac tgaacgagaa aaaacaataa
    11941 tggtagttat actccgattt cattcaggaa ttttatgctg gattttttaa agaagctcgt
    12001 tgtaatggct tatatgctca agggagggca aaccaaagtc accgtcagaa atcagccgct
    12061 cgtgatttct gtagatcatt ggagagtctt ggggcgcgca aaaaattatt cgatcaaaga
    12121 acccgataca ctagattggt tggatggttt tgaaccagga acttgttatt ttgatatcgg
    12181 tgcaaatatt gggcaattct cgctttatcc agctaagaaa tatgggcaaa aagtagaaat
    12241 ttatgctttt gagccccaat caaataatta ttacgcactg aataagaata tttatcttaa
    12301 tcagcttaaa gactccataa cggcctactg tgttgccata tcgggtgaaa gtaaatttga
    12361 taaattgtat gtgccaaagt ttattcctgg cggtaatcgt tctcaattcg gtgaagaatc
    12421 gcaagcagct atgggggtgc caacttctca tattcaagga atgtttggaa tcacgttaga
    12481 tgacttatgc gagaaatgga attttcctta tcctcactat atgaaaatcg atgtagatgg
    12541 cattgaaatt tctattctta gatcagcttc taaggtactt aagcacccaa atcttaaatc
    12601 tgtcattgtc gagctgggta ccccgcttga gcaacaagaa gcaatcgcta tcatgaagca
    12661 agctgggctt gagttgaaac atcaatccac tcgtaattgg ggagaaactt gttttatttt
    12721 tgagagaacc agcacggcat gatatttcgc tttatctgtt attgctgatt gtttgtgaaa
    12781 gcactctttt ttcttaggca ttacaacgat atcgatcaca tgacgcctgt catagcaaaa
    12841 tggatcgaga cgggtcatat ttgtgatgtt gtaatgatta gtaagaagcg ctttgatggt
    12901 gattaccgaa ttgaatatct aaaacatttg cagggagttc gtctagcaca tattcgagac
    12961 ttactgccag tttttgattt tttcatgtgg cgattgcaaa cactacttct tgtcagaagt
    13021 tcgtaccgct catttcttaa acctttagta atgtttctgg ctacgatttt tcggcaagaa
    13081 aaacgaaaga aagtttggga gaaagtatca cgctatttat taaagcgtag tttcaatgat
    13141 ctaaacaatg gggttgttgc atttgattgg atcgaacgta attcagaaac ggctgttgaa
    13201 tgggttgatg tgtttgttaa tcttgcacgt aaccaaggtt tgggtgtgat ttcgctgccc
    13261 cacggagata gtccacacgc aagtcagttg atccgtcatg gggaatggtt gcttaagcct
    13321 gatgtatcgt tttctgctgc tggaatgttt gatcgagtgg tcgtgccaaa tgcgttatgt
    13381 gcaatacgct ttcgtccgtt tctagatgat cgaaagctag tggtgctagg gtcacctcgc
    13441 tattgtgaag aatggctaac aaaacttaag tcattattac cagaatctcc attgcaaaat
    13501 tccagaagtc ggttaaagat tgtgctattc ctcagaaaag ctaatttcac gacattttgg
    13561 gaagaattaa gtgaagttgt tcaaatgttg tcaacgtttg aggaggttga attagcgatt
    13621 aagcctcata cgcggggagg atggaaacaa tctttcacta aaagtgcggc aatccagcat
    13681 ttaaagaatg tcaaaattat cgagagtgat atccactcaa tccacctgat tcattgggca
    13741 gatgttgtga ttgatctagc tacttcgatc gcttatgaag cgataagagc aaaaaaaccg
    13801 ttgctcgctg cagattatct tcatgctgga cgctcagcag ctgctcagta tatgccggag
    13861 actgagctgc gctgtcgtga tgatgtttac gaaagaatcc atcattttct tcagaatggc
    13921 tgtgaaaatt attacatcga gggaaatcgg cagaaattca ttacggagat gttggaatct
    13981 cctggaacag atgttttgcc tcattacgtt ggactgcttg aagcgttagc ttcaaaataa
    14041 ttctttccta tctaaaaatt ttatctgaat tcaagctatc gttaaacgat gggatacctt
    14101 aatgagtgaa aaacaaatca gcatgaccta tttattgagt gaacgtttga ttagcgtcga
    14161 atggcgggag cgatttattc gattttcctt gattttcttt tgctttgcac tctggcagaa
    14221 atggtcattc ttttatgctt ttttcttgtt atcaggggca tggatactcg atggcgggct
    14281 aagaaacttc aagcaactga taagagaacc gctcgttcaa ggaatattgg ttttttgtag
    14341 tgttgtggcg attgggttgt tgtggggtga ttacattgag accaatcaga ataagtggaa
    14401 gaaatatttt attctcttaa cgtttattcc tttttttgcg ctgctgaata aagatagcgc
    14461 gcgttttcca tgggtttcta atacgttctt aatcgtctac ggtagtgtgt tgttagtcgg
    14521 cgcctatcag cattttgtgc aagggttaat cggtgttccc gcattagata tttcttacct
    14581 tagtttttct gcgatattgg gcgtaggtgt aattaccaca atttttttag ctcaagtgag
    14641 ccaaacgata ttgcagaata tgatctggtg gttcatttct gcgctaatgc tgctgctaca
    14701 gttcaatcag ggagcaagaa ctgtcttgtt cgcaacgatg gtagctgcaa ttattctgat
    14761 cttttttatg ggccagaaga aaagaaaaat gaggttaatc ggaatttcgt catttatcgg
    14821 tttagcgctg ctgcttatgg ctacgaattc agtgatgcaa gaacgattga gtctcgtgag
    14881 tcatgattgg gctgcgatta aactaggcaa ttatgaaact agtctgggtt atcgattagc
    14941 aatatgggat attggtttga atggtataca agaacagcct tggataggtc atggaaccgg
    15001 taatccagag gtttattttg aaaaagagac tcaaacgtat aaagatggaa tttatcgtaa
    15061 cttgcccgca tttcaaaaaa cttcgcatta tcataatgat tggattgaag ttggaatgca
    15121 ttggggtgga ttgggtatta tggcgttggc ttttttgata ttttcttggt taagaatatt
    15181 taaagaaaat caattagcta atctaggaat tgcgtttgtt agttatatag tgctggctgg
    15241 ggtgacggat acttttcttc tctataatag agtaccgccg atattattgg tgattacagc
    15301 attgctggta tgctggcagc gctcaaaaat gaagagctga ataagaaatg acaatctttg
    15361 ttacgctagt gtaggactac ataagggatc ctatcatgaa aagatttgaa ccgttacaga
    15421 gcgagcatct agttgacttg attccggtta atccagcgat agaatgctac atgcaaggat
    15481 taattgagag aaggacagat tctgtattag ttgagatgga ggccttagca aagcaaaaaa
    15541 attttcccat tgtaggtcgc ctagttggca cgtcattaga agttttagct aagatgatta
    15601 acgcaaaacg tgtttttgag tttggtagcg gctatggcta ttctgcatat tggtttgcac
    15661 gtgctgttgg attaaatgga caggtgatct gcacggacaa taagcttgcc aataaagagc
    15721 aagcagaaat ctttttatca tccgtcgatt tatggaaaag ggtaactttc tatatcgggt
    15781 atgctcaagc ggttttttcc caaacggaag gtttctttga tatctgctat aacgatgtag
    15841 ataaaagtga atatccgtca gtatggaaaa tggctagaga aaaaatcaaa ccaggtggtt
    15901 tatatattgc agacaatgta ctctggcatg gaaaagtaat cgcagaaaat aataccgatg
    15961 aatggacgca agcaattctg gagcataatc aattgatttt tgacgatcct gagttcgatg
    16021 cgtttattaa tccaactcgt gatggggtta tggtagcaag aagaaaaatg gcataataag
    16081 cgcactggat tcagtcgatt ctgtaattga attgattttt atttacgcat taatttttta
    16141 aggatagatt tatgcaaatt catgtctatg atacttacgt taaagctaag gatggtcaca
    16201 tcatgcattt tgatgtattt acttccgtta gagacgatca gaaagcgatt gggtatgcaa
    16261 aacagtggct aacttcgatt ggtgaggaag atgctattgt ttcaagtaag gaatgtaggt
    16321 tttgtcatag ccaaggagcg ccagatgatg tgattgaagc cattaataaa gatggttatt
    16381 tcatttacaa aatggaagga tgcagataac ttctgggcag ttagttatta acccaattcc
    16441 aaggattgat agatgaaaca agtacctttt aagcggagtc tttttattgc tttattctta
    16501 gcatgtggat tgagccatgc tgctgaccct aaaatgatag gtgaatttaa tgattggaca
    16561 gcctatgttt acatggaagg taagaataaa gtttgttata tggtgagtaa acctaaaaaa
    16621 gaagaaggga gctacacgaa acgtggtagc gtatatgcgc tgttgacgca tcggcctgct
    16681 gaaaacagta aaaatgtttt tagctttatg gctgggtacc cttttaagaa agaaagtgaa
    16741 gttacggtta gtatcggtaa ccaaaacttt aagcttttta cagataatga gactgcttgg
    16801 gctcaagata gtgatacgga taacaaaatc gcgaatgcca ttcgtaacgg cagtgcgttg
    16861 atcattaaag gtgtgtctgc tagggataca gcaacgacgg atacgtttgg attgaagggt
    16921 tcgtcatcag cttatggcgc aatttctaaa gagtgcggca ttaagtagca gcaatttatt
    16981 aatatgcgtc ttcgtgttta ggattaatag tttaaatgga tgaggtcata acgatttatc
    17041 aaaaaccaac ttgcagtaag tgcagagcta cccttgaact gcttactcaa agtggtgagg
    17101 aattccaaag tattaattat tatgaagatc ctatttcaga ggatttattg cgcgagcttc
    17161 ttcaaaaact caatatgaca gcacgcgaaa tttttcgatc tgatgagcca gcggctagag
    17221 gtttgcagtc agcgaccgat gaggagctat tgaaacgaat ggctgcaaat cctgatttga
    17281 ttcagcgacc tattgttgtc aggggtgaga aagcgatctt ggctagacca cccgaacaag
    17341 ttttacagtt attaaaaaaa taggattgct tcattgcgct atgtatctgg atttcatcga
    17401 tttaaactaa tcagcaagct tataggatca tttttgagtg attgttttat cctagttgta
    17461 acggatggct aatgtagggt aaaatcggcg gtttggattt cagattaaat tagcagcgat
    17521 tattctattt cataagatat tttggttaaa tagaatttag aatgttaatt taagaaatta
    17581 tcgtaaagtg agagatactt aagtgaagac cttttcagca aaaccattag aagttcaaca
    17641 tgattggttt ctaatcgatg caaccgataa agttttaggg cgactggcaa cagcgatcgc
    17701 acatcgttta cgtggaaaac ataaacctat ttttactcct catgttgaca ctggagatta
    17761 catcgttgtt atcaatgttg ataaaataaa agtaactgga aataaagcag aggataaaaa
    17821 gtactatcgt catagcggtt atccaggagg aatttatgaa acaaccttta agaagatgca
    17881 tgctcgtttt cctgcaagac cgcttgagaa agcagtaaaa ggtatgctac caaaaggacc
    17941 gctgggatat gctatgatta agaagctgaa aatatatgca ggtgatacgc atccccatag
    18001 cgcacaacaa ccaaaaaatt tagagataaa ggcgtaattt atggcgataa attataatta
    18061 tggaacgggt cgaagaaaaa gctcagttgc tcgagtattt ataaagccag gtaaaggtgc
    18121 ttttgtcgtt aatgataaac cactcgatga gtatttttct cgggaaacgg gtcgcatggt
    18181 tgttcgccaa ccgctggaat taacgggaaa tactgctaat tttgatataa tggttaatat
    18241 tcatggcgga ggagaatctg gtcaggcagg cgcaattcgg catggtatta cacgtgcgct
    18301 cattgattat gatgaaacat taaaaccggt tcttagtaag gcagggtttg taacaagaga
    18361 tgcgcgtgaa gtggaacgca aaaaagttgg tctgcgtaaa gctaggcgac gtaagcagtt
    18421 ctctaaacgt taaacgtatc tgtttctcat taaagccgcc tttaccaggc ggctttttat
    18481 tttgtacatt ttgttttttt cattgattca atatgattaa agtaggaatc gttggtggaa
    18541 ctggttatac aggagtcgag ttgctgcgta tcttagcgca acatccagaa gttagtctga
    18601 aagtgattac ttctcgacaa gaagcaggtt tggctgtcga tgagttattt ccgagtttaa
    18661 gaaaatatgt ttcattaaaa tttgttgatc ccgaaaaagc caagctagat aaatgtgata
    18721 tcgtgttttt tgccactccc aatggagtag ctatgcgcca agctcgagag ttagtggatg
    18781 cgggtgttaa ggtgattgat ctatcagcag attttaggat aaaagatatt tctgagtggg
    18841 aaaagtggta tggtatgtcg catgcctgcc ctgaacttgt ttctgaagcg gtatatggat
    18901 taccagaagt taatcgcaaa cagataaaaa aagcacgtat catcgcaaat cctggttgct
    18961 atccaacagc ggttcagtta gggttcttac cattaattga agctaaggta attgatacca
    19021 attcattaat tgcagataca aaatcaggtg tatcaggcgc tggtcgaaaa gcagaagtac
    19081 atacactttt tccggaagcg gctgataatt ttaaatctta tggcgtaccg ggtcatcgtc
    19141 atcttccgga aattcggcag ggtttgtcca atgtagcggg taaactggtg ggcctaacat
    19201 ttgttccaca cttgactccg atggttcgtg gaattcatgc tacgttatat gcgaaattga
    19261 tgagtattaa gactaaagtt gatcttcaaa cattgtttga agaacgctat aaaaatgagc
    19321 attttgttga tgttctacct gcaggcagtc atcctgagac tcgttcagtg cgaggctcta
    19381 actattgtag aattgcggtt catcgaccac agggcggaca aacagtcgtg gttcttgcgg
    19441 ttaccgataa tttagttaaa ggagcagccg gtcaggctgt tcaaaatatg aatttaatgt
    19501 ttggtttttc tgaaacatta gggattgaac atgttccaat attaccgtaa agcaaactta
    19561 attcgttttt ttgcttacaa atatcaatga ataactgcga atgggaggag catagatgca
    19621 attaaattct ttagaagaaa atcaagcgat acaaggccaa cttgttttta ctgatagtgc
    19681 tgcgagtaag gttaagcagc ttatcgaaga agagggaaat cctaacctaa agctaagggt
    19741 ttttgtcagt ggtggtggat gctctggttt tcagtacggt tttacttttg atgaattaga
    19801 aaatgaagat gatttggtaa tcgagaaaga gaaagtcaag ttgctgattg attcaatgag
    19861 ctaccagtat ttgattggtg ctgaaatcga ttatgtagaa aattctcaag gtgcccagtt
    19921 tgtcatcaag aatccttctg ctgcaagcac ctgtggctgc ggctcttcct tctcggttta
    19981 gaaaatattt ttgacacaaa cgtaagcggt ttatttgaat atacactatg ccatcattcg
    20041 atattgtatc cgaggttgat aaaacagaag tcaagaatgc gattgatcag accaataaag
    20101 aagttggtac gcggttcgat tttaaaggtt ccgatgcacg tgtagaacaa gctgaaatgg
    20161 tcttaacgat ttatgcggac aatgatttcc aattgagtca ggtgcaagat attcttaata
    20221 ctaaattaag caagcgaggc gtagacatga gatgccttga tgttagcgat aaaattgaaa
    20281 aagtgagtgg gaataaggtt aagcgttctt gtactgtaaa ggtagggata gaaaccgagt
    20341 tggcaaaaaa aattattaaa ttgattaagg acagtaaaat taaggtacaa gcaagcattc
    20401 aagctgatac cgtgcgtgtt tccgggaaaa accgtgacga tttacagcag tgtattgcat
    20461 ttgtgaagaa gacgatcgca gaatttccgc tacaatatca aaattttaga gattagtacc
    20521 cttaggagaa ttatttcgca gagttaatcg taactcttgc aaacttacgt ttgccgactt
    20581 gaatagttat agtttttcca gaagcaattt ttagggattt atcactgatt ctatcaccgt
    20641 caatctttac gccgccttgc tcgatcatac gaaaagcttc gctcgtgcta ctggttaaat
    20701 caacttgttt cagtagctga ctaatcgtta cctcatttcc gttaatctgg atagttttct
    20761 gagggatatc atctggcagc aaaccattct taaaacgtgt ctcaaagtcg attaatgcat
    20821 gttctgccgc agctttacta tgaaaacggg taacaatttc ctgagcaaac gttaccttga
    20881 tatcccttgg gttttggcca gcatcaactg cttctttcca gtta
//
LOCUS       DAOQKQ010000025        20710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_1121_length_20710_cov_5.960107, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000025 DAOQKQ010000000
VERSION     DAOQKQ010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 20710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20710
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1121_length_20710_cov_5.960107"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PKZ49_02835"
     CDS             <1..352
                     /locus_tag="PKZ49_02835"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HND35526.1"
                     /translation="LAFTGFPIGMVLGFYPVTHRAMIASITPIILPVTNTKQLSAEKI
                     KQLNKTAFHVFQLDGTAYPGSSGSPLYDSDSGEVYGVVNLVFVKGKKESALSNPSGIS
                     YAIPANFVTELLKY"
     gene            494..1852
                     /locus_tag="PKZ49_02840"
     CDS             494..1852
                     /locus_tag="PKZ49_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03013 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HND35527.1"
                     /translation="MALICTEICLLMIVFYIGAGIRYASWENIEINLFLSELPGALTF
                     TLVTIICMAAMGMYQSDFKLDFESILLRLMPAFALSFAIMTLIFYVLPDLYFGRGLLA
                     IAMVLAFALLLLLRMLFFRLPGVEFFRPRAIVLGTGNSAHELYKLIEEKSFFRNHNIV
                     GYVPFYGEERKIPVTDIIEKSSNLSVLATQKYVSEVIVATQERRGGGFPIQELLECKM
                     NGINVMDIATFYEKERGYIRMDSLQPSWLVFSRGFDQSLIRSLIKRIFDLCASILLLI
                     VTFPIMLITMIIILLEDGKPIFYRQERIGRGGSIFSVIKFRSMRNDAEKGGKPQWSTQ
                     DDPRITRIGKIIRKLRIDELPQIINVLKGEMSFVGPRPERPYFVDMLIAQVPYYNIRH
                     SVKPGITGWAQVRYPYGSSVEDSIEKLQYDLYYVKNHSLFLDLIILIDTIGVVLLGKG
                     GR"
     gene            1871..3943
                     /gene="prsK"
                     /locus_tag="PKZ49_02845"
     CDS             1871..3943
                     /gene="prsK"
                     /locus_tag="PKZ49_02845"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM system histidine kinase PrsK"
                     /protein_id="HND35528.1"
                     /translation="MLTLPISALSYAAASLGYFVLFILLVTSWRGKFYGILLAIACIT
                     SSLWAANISAQSYWGYPSIFSIEILEIIKNLSWSLFLIILLGPFQKNESKSSTLKLKS
                     SVIGILLLYLVAIFTAIYSQDTKGVFSGGYQTDYLVSVIMPLIGMILIEQYYRNTPVD
                     QRWGIKFFCLGIGGFFVYDFFLFSDALLLGKVNTDIWDARGWVNLLTVPLVGLSAARN
                     PKWSVGISLSRQILFYSSTLFGAAVYLLAMAGVGYYIRLSGGTWGTVLQLTFLFGAIV
                     LLLVILSSGTTRSWLKVFISKNFYSYNYDYREEWLRFTRTLSEESLELRTRVIKSLAQ
                     LVESPAGILWYKSEQGEFKLTASWNFSLKTQDIADHSNDEFCQFLNQKRWVIDLGEYR
                     LHPQKYSGLALPSWVLANEKLRLIIPLILNNELVGFIALAEPRSIIKLNWEVRDLLWV
                     ASTQATSYLVQYEVTTALSLARQFESFNRMSTFVVHDIKNLIFQLSLLLTNAEKHKEN
                     PEFQKDMMETVSFSVNKMQRLLEKLNSDRETVKTECVFLDQLLRQIVQRKLICEPQPS
                     LDISDEKLMVKADLTRLERVIGHLIQNAIEATSKTGFVKIKLSKNEDKALIKIEDNGH
                     GMSEAFIKNKLFRPFETTKTAGMGIGVFESKEYISELGGQLEVSSREFIGTIFLITLP
                     LMENQKGI"
     gene            4145..5503
                     /gene="prsR"
                     /locus_tag="PKZ49_02850"
     CDS             4145..5503
                     /gene="prsR"
                     /locus_tag="PKZ49_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM-box response regulator transcription
                     factor"
                     /protein_id="HND35529.1"
                     /translation="MKTSDDKRKLLVIEDDPGLQKQLRWSFDNCEVFVAGDRESALVQ
                     VRRHEPSVVTMDLGLPPDPDGASEGLATLKQILALAPNTKIIVLTGNQNRENALKAIE
                     LGAYDFHQKPFDSKMLSLIIDRAFYLHALQRENRSLLLSHSDSPISGIVTRDPNMIRM
                     CRNIERVAPSDVTVMLLGESGTGKEVLAKALHQLSTRKEGRFVAINCAAIPESLLESE
                     LFGHEKGAFTGAIKQTLGKIELAQGGTFFLDEVGDLPLSLQAKLLRFLQERVIERIGG
                     REEIPVDVRVVCATHQNLKKLIEEGRFREDLFYRLGEISIKIPPLRERLGDAVLLAHH
                     FRNKFATKEGRHSMSFSQEALSAIESYSWPGNIREMENCIKRAVIMAEGPQIALDDLG
                     IQESAKPQEPLNLREIREKEECKAVVRALAKVDGNIARAAELLGVSRPTLYDLMNRHG
                     LK"
     gene            5566..7143
                     /locus_tag="PKZ49_02855"
     CDS             5566..7143
                     /locus_tag="PKZ49_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA ligase (AMP-forming), exosortase A
                     system-associated"
                     /protein_id="HND35530.1"
                     /translation="MPHLFHELIYKAANCGAKSEALISQQHRLDYLELAEQTQKLATG
                     FIKIGIARGERLAVYLEKRVEGVTALFAASAAGGVFVPINPLLKAEQVSYILSDCNVR
                     ILVTSISRFNLLKSVLTNCHDLHTIIVIDDNVELPSVKGLNLIRWQEVIAEKASCYTH
                     RTIDSDMAAILYTSGSTGKPKGVVLSHRNLVTGAKSVSTYLKNNSDDRILSVLPLSFD
                     YGLSQLTTAFYVGATCVLMNYLLPRDIMDEVIKEDITGLAAVPPLWIQLSQLKWEGLT
                     SLRYITNSGGAMPKATLDQLRGLFPKTSIFLMYGLTEAFRSTYLPPEEINKRPDSIGK
                     AIPNVEVLVLREDGSQCAPGEPGELVHRGPLVSMGYWNDVEKTSACFRQLSPRQSGLT
                     IPEIAVWSGDTVRTDEEGFLYFIGRRDDMIKTSGYRVSPTEIEEIIYSTELVAEVAAV
                     GVPHPVLGQAIVVVVVPRQDVNLNADVLLSACKQALPAYMLPALVDIRKHSLLRNPNG
                     KIDRKSLANELQHSFIN"
     gene            7157..8392
                     /locus_tag="PKZ49_02860"
     CDS             7157..8392
                     /locus_tag="PKZ49_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase, exosortase A
                     system-associated"
                     /protein_id="HND35531.1"
                     /translation="MTNNRPKHASFDQFPIRDHCIQIGGMSLNRLTQRVGSTPYFAYD
                     RKLITERILFLRQCLPNKIKLHYAMKANPMPAVVQHIAGLVDGIDVASLGEIKVALDT
                     GMLPSKISFAGPGKNEHELSCAIAAGIVINIESELELERIVALAERLGITPKVAVRIN
                     PDFELKSSGMKMGGGPKQFGIDAERAPIVLRRIVEFGIVFEGLHIFSGSQNLKVEAIQ
                     DAHEKTLQLAVRLAKESSVSIPSLNMGGGFGIPYFPGESPLNLVAVGENLQHLLNKIE
                     SELLDTQFVIELGRYIVAEAGIYVCRVLDKKNSRGQLFLITDGGMHHHLAASGNFGQV
                     IRKNYPVTVGTKAETAETEVVSVVGPLCTPLDLLADQMELPKAEIGDLIVVFQSGAYG
                     LTASPISFLSHPIPLEVLV"
     gene            8454..10301
                     /locus_tag="PKZ49_02865"
     CDS             8454..10301
                     /locus_tag="PKZ49_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HND35532.1"
                     /translation="MSGVCGWVGYGQSVVENQQISESMAQVLTRFDHSRSQIYVDENS
                     SLAIAASEQQLHYSHKNDIIVGIWGHAKFNNDELNQLILSQGIAGVLLDQWVNNGPSV
                     IESLLGEFICCLVNAKSHEVFIAVDRMGTRPLYYQILENGLLFGTSLDAMMRHPLAKD
                     EISNQGIYDYIYFHMVPGPNTIYREPRRLLPGEYIYYKHGTLTVERYWNAQFRENEAQ
                     SFSELKQSFISLLHTSMEDAIAGYQHVGAFLSGGTDSSTIAGMLQEVTGKPAKTFSIG
                     FDAPGYDEMDYARIAARHFSTNHHEYYVTPEDVASAIPQVAAIFDQPFGNASAIPSFY
                     CAQMAKKDGIDLLLGGDGGDELFGGNVRYAKQYVFSLYERIPSVLREKIITPFALSTL
                     AGSNISLFRKIHSYISQASIPMPHRMETYNLLIRYGYQDVFTEDFLSNIDINEPASQI
                     NQTYHKAETTTLINRMLAYDWKYTLTDNDLPKVTKACELAAVDVAYPFLNSEMVSFSE
                     RLRPELKLKKTKLRYFFKEALRDFLPKEIITKQKHGFGLPFGIWLQENKLLQAQAADC
                     LTDLKKRHIFRVNFIDKILSQYLHQHASYHGTMVWLLMMLECWYKQRSR"
     gene            10691..11482
                     /locus_tag="PKZ49_02870"
     CDS             10691..11482
                     /locus_tag="PKZ49_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM/exosortase system-associated
                     acyltransferase"
                     /protein_id="HND35533.1"
                     /translation="MSDIADAFHEYFEIVVADTLELKKEVYRLRYRIMCLNTGIFDTT
                     LYPDELEKDEFDDHSIHILLRHKPSNAFMGTARLILSDPSPLANRAFPVELHTQFYPA
                     FSNMNIIREHTVEISRFAIHSDFFRRKDDFRFSVDKAASQGIRKNERRRFPHPMLGLA
                     VGIIQLCAKHDFFFWFSAMDPALNRLLGFYGMQLNPIGPIVDYYGPRRPYHVCVIDVL
                     ERMYVNYPEVWELVTDNGQIWPSDLQSVRERNQHILLASNEMVTC"
     gene            11498..11956
                     /locus_tag="PKZ49_02875"
     CDS             11498..11956
                     /locus_tag="PKZ49_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35534.1"
                     /translation="MVARNRFNYCYRIVSLFFFVFGVSYFATHTYANDSVEAVIHPSV
                     ELKSYSRNSLRAAFGMRLKTWPNGMPVRVFVMPDDAVLHTIFTKEKLNIFPYQLRAGW
                     DKLIFSGTGQAPFLVRTEEEMRERISSTPGAIGYLKRANIDESIHVLNIE"
     gene            11925..13226
                     /locus_tag="PKZ49_02880"
     CDS             11925..13226
                     /locus_tag="PKZ49_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35535.1"
                     /translation="MKAYMYSTLNKQYSILNYTMMCRLASVVLLLIIMMQEGIAQDVK
                     GIRVHGFLSQGYLKSTGNNDVFGNSSGNGSFDFREIGANASYRPLPNLQFSGQLLSRS
                     AGEDSKGGIRIDYGFVDYTAISTESMDFGIRLGRTKNPLGFYNDTRDVPFTRPSILLP
                     QSIYFDRTRNLGLASDGAQIYGESRNSWGDLNAQFGVVFPQAGDRSTEFAILQADRPG
                     GITPHLSYIGRLIYEYDGGRVRLALSGAQVNTGYDAAQVDPFSGLGSFQFTPIIFSAQ
                     YNTERWSLTSEYAIRHIERYDFANRSARDLNLTGESYYFQGIYRFSPNWEGVLRYDAY
                     YADRSDRDGKDFAKFIQIQAPDFQDNFPSYSRFAKDITVGLRWNVTPSIMLRAEYHYI
                     NGTGWISSLDNRAPNTTLQRFLPAITDQHWNLFAFQASYRF"
     gene            13238..16177
                     /locus_tag="PKZ49_02885"
     CDS             13238..16177
                     /locus_tag="PKZ49_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HND35536.1"
                     /translation="MKLSHLVSLTRLDREIHKTLSKFFSLKWKISLLSSLILLIVVTM
                     FSVINYRSLIDNVDYQSSLQYQRYVTEVENLIEQTSNNSRQLIRLIPFLKEMDTALVL
                     QDSAQITQAFEQHWSLFQLHNGIETIRFYDQSDQLLVGWDNFEEDSQRDALILEQVHD
                     VNSQELPASLLICIEMCLQYTIEPLLIDGVRAGAMVIGASLADVFLGFKRTFGSDIGL
                     LLKMKADGVDIQQNDVEFSNWGVRLAALTNREKNLEILHQIAQSHPTIQDLRVGIHTE
                     WDNRHHRIKLLSLRGVNVLDRAYLVVLTDITPTILSIRDSTWRIIIIGLVGLLVSELI
                     LFAILSLPLTRLKHIVFTLPLLAGGSFGIFRATLASVSRKRWLKDEVDLLYEAAVALS
                     HKLEKLENKVAYRTKLLVQQRDELSKEKDFIAHLLDTAQVIVLTQNVSGKILMLNAYG
                     ELLMRYSKEEIQTKYFLEVLAPDNDQQYNLVNYLEEIRRGHRDQLRHEAMTLCNDGSK
                     RHIVWLHSRLTWQSETDPSVLSVGLDITEYKRVEGHLAWLADHDPLTNLFNRRRFSEE
                     LEQMLSWAERYQHPGALLFFDLDRFKYINDTSGHQAGDALLKMVAEMLSRTIRTADVT
                     GRLGGDEFAVILPEISAGGAIEVAKKILLRLSETQLNVNGRTYKIAASVGIALFPEHG
                     KNVHDLLAAADLAMYQAKEIGRGTWHLFSNDDKTRERLYTLVHWKEKIEYANLYDHYL
                     LYFQPIMSLHDRAITHYEVLLRMHDNDGTIHSPAAFIAAAEHTGLIHAIDHMVLRKAI
                     TQVAKINSKSTKPIRFSINLSAHAFNDPELLPVIRHELTSSGVDPTLLFFEITETAAL
                     DNLPGTRNLLREIKKLGCGFVLDDFGVGFSSFYYLRELPVDAVKIDGSFIRNLANNPD
                     DQILVRALCSVAKGFGKKITAEFVESEEILMLLGKMDVDYAQGYHVGKPAAAEDIFRL
                     FEENEIS"
     gene            complement(16187..17686)
                     /gene="lnt"
                     /locus_tag="PKZ49_02890"
     CDS             complement(16187..17686)
                     /gene="lnt"
                     /locus_tag="PKZ49_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HND35537.1"
                     /translation="MDLKSKSFLALILGTLTVLGFAPFYYFPVPVITILLLFHLWRSS
                     TQSWHSAVIGFSFGMGLFGAGVTWLYVSLHDFGGMPLPIAVVALLLLCAYLSLFPALT
                     GWFATKIRHIHFSAWAACVSACWVMSEWLRGTLFTGFPWLTLGYSQVPTSPLIGYAPI
                     LGVYGISLLLVFSIALYIAWPKQKNLNHYLWILGGIWVSGYGLQHYQWVDPEGSPITV
                     SLLQGNISQDLKWREDHLVSTMNTYADLVLESNSRLIVTPEISIPLFYEDVPTDYLTL
                     LSDHAKQNQADVLIGFAETVPHSIDRNEYYNSMFSMGSSPEQRYRKHHLVPFGEFIPF
                     KPLFGWIIQVLNIPLSDFSRGSLDQQPLDLAGQKIAINICYEDVFGEEIILQLPQASI
                     LVNVSNDAWFGRSIGPRQHLQISQMRALETGRYMLRATNTGVTAIINERGQIVQELEM
                     FVTQGLHGTAQGFKGATPYVQYGNTLVLTIIGLCLSLGGFIAFFRKEND"
     gene            complement(17749..18126)
                     /locus_tag="PKZ49_02895"
     CDS             complement(17749..18126)
                     /locus_tag="PKZ49_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6164 family protein"
                     /protein_id="HND35538.1"
                     /translation="MSKLLFKLKNVPDDEADDVRQLLVDHQIDYYETTAGNWGISMPA
                     IWITHDGQFGEAKQLFDAYQTQRALNVRAEYAQLKKEGKHETLLGRVKENPLLYFIYT
                     LAVILLLYLPYKLILEIAEKLQN"
     gene            complement(18255..19160)
                     /locus_tag="PKZ49_02900"
     CDS             complement(18255..19160)
                     /locus_tag="PKZ49_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HND35539.1"
                     /translation="MQQQRYDQEFNFFLIISMVFSFGLVLFTSPVAAHDRAKALEQEV
                     LELKKRLISIQDELSQIRTDSSRVEQQVIESKQAIKEERVIVQQKIESEKEVIKKAIL
                     EESPRNMIFFRGGWARQDHHHNGISVQSSVAPVGAQEQAPQNAWYVGAGFDFGLTKDT
                     WGLIPKTELIAELMFEYKQFGTVLGNVLANEPTQLAGGDLNPRQVTSSTVTVSAAPKI
                     KFLEGYRLRPWIIPAGLAIHIISPPSETITFFQPAVMFAAGADYKLWKNIVLGVDARY
                     HLMGGHLDGIRLNGLTLGGYLGIGF"
     gene            19405..20271
                     /locus_tag="PKZ49_02905"
     CDS             19405..20271
                     /locus_tag="PKZ49_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HND35540.1"
                     /translation="MNEWMDKVGEMNKSILITGCSTGIGYVTAHKLRERGYRVFATAR
                     RAESVAMLQAEGFESLQLDLDDSNSIQQAFAEIMQRTGNELFALFNNGAYGIPGAVED
                     LSRAALRAQFETNVMGWLELTNLVLPIMRNQGYGRIIQNSSVLGFAAMPFRGAYNASK
                     FAIEGLTDTLRLELMGTQIYVCLIEPGPISTQFRANALKALQKFIPVEQSVHREKYQA
                     VLSRLNKPGPAVPFTQPPEAVAKRVVEALEAKVPQPRYYVTFPTYLFGFLKRVLSIRL
                     LDRILAKAGTQN"
     gene            complement(20526..>20710)
                     /locus_tag="PKZ49_02910"
     CDS             complement(20526..>20710)
                     /locus_tag="PKZ49_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HND35541.1"
                     /translation="PTTESVTYLAPGMMFGAGFDYRIWKEFFLGADARYHETFGKTDG
                     VNVSGLTAGAYLGIGF"
BASE COUNT         6001 a         3810 c         4546 g         6353 t
ORIGIN      
        1 gcttgctttt acagggttcc caatcgggat ggttttaggg ttctatcctg ttacacatag
       61 agcgatgata gcttcaatta caccaattat tttacctgtt acgaatacta aacaattgag
      121 tgccgaaaaa ataaagcagt taaataaaac tgcttttcat gtttttcagc tagatggcac
      181 tgcttacccc ggcagtagtg gaagtccact ctatgattct gattccgggg aagtttatgg
      241 ggttgttaat ttggtttttg taaaaggaaa aaaagaatcg gcactcagta atcccagtgg
      301 gattagctat gctattcctg ctaactttgt tacagaactg ttaaagtatt agtaatggta
      361 atctcatcct atggaattat aagtaagtag ggattatcaa atgaattctc tattattatt
      421 tctataaaat tttttcttta ttaggtgagg aaatttgatt cgcgtatata accattacat
      481 ttctcgatta actttggcac ttatttgtac cgagatttgt ttattgatga ttgttttcta
      541 tattggtgct ggaattcgct atgcttcttg ggaaaatatc gaaatcaatc tttttttgtc
      601 tgaattgcct ggcgcgctga cctttacgtt agtaactatc atctgtatgg cggctatggg
      661 tatgtatcaa tcagatttta aattagattt tgagtcaata ttattgcggt tgatgcctgc
      721 gttcgcattg agctttgcaa taatgacttt gattttttat gttttacctg atctttattt
      781 tgggcgtggt ttgcttgcta ttgcgatggt tcttgctttt gcattgctat tgctgttaag
      841 aatgttattt tttcgattgc ctggcgtgga gttctttcgc ccgagagcaa tcgtacttgg
      901 aacgggaaat agtgcccatg aactctataa actgatcgaa gaaaaatctt tctttcgtaa
      961 tcataatatt gttggatatg ttccttttta tggggaagag cgaaaaattc cggttacgga
     1021 tattattgag aaaagtagta atctaagtgt tttggcaacc cagaaatatg taagtgaagt
     1081 gatcgttgca actcaggagc ggagaggagg agggtttcca atccaagaat tattagaatg
     1141 taaaatgaat ggaatcaatg tgatggatat agctaccttt tatgagaaag aaagaggtta
     1201 tattcgaatg gattcacttc agccaagttg gttagttttt agtaggggat ttgatcaaag
     1261 cctgattcga tcattaataa agcgtatttt tgatttatgt gcaagcatac tgctattgat
     1321 tgttacgttt ccaatcatgc tgatcacgat gatcatcatt ttattagaag atggaaaacc
     1381 gattttctac cgtcaggagc ggattgggcg tggaggaagc attttttcag tcattaaatt
     1441 tagaagtatg agaaatgatg cggaaaaagg agggaaaccc caatggtcta ctcaggatga
     1501 tcctcgtatt actcggattg gaaaaataat tcgtaagctg cgaattgatg agcttcctca
     1561 aatcattaat gtgttaaagg gtgaaatgag ttttgttggt ccgcgccctg aaagacccta
     1621 ttttgtagat atgcttatcg cacaagtacc ttactacaat attcggcata gcgttaaacc
     1681 cggaataacg ggttgggctc aggttcgtta tccctatggc tcatcagtag aagattccat
     1741 cgagaaatta caatacgatc tatattatgt gaaaaaccat agcttgttcc ttgatctcat
     1801 tattctgatc gatacgatag gcgttgtgct attgggtaaa ggtggtaggt aggaataatc
     1861 agttgatcat atgcttacgc tacccattag tgcattgagt tatgccgctg catcgctagg
     1921 atattttgtt cttttcattt tgctggttac aagttggcgt ggaaagtttt acgggatatt
     1981 gttggcaatt gcgtgcataa cgtcatcact ctgggctgct aatatctcag cccagtctta
     2041 ttggggttat ccttccattt tctcgattga gatactagaa ataattaaaa acttaagctg
     2101 gtcattgttt ttaattattc tacttggtcc ttttcaaaaa aatgaaagta aatcaagtac
     2161 attaaaactt aaatcatccg taatcgggat cttactactc tacttggttg ctatttttac
     2221 tgccatttac tcccaggata cgaagggagt attctcaggt ggatatcaaa cagattattt
     2281 agtgagtgtc atcatgccgc tgataggtat gattttaatc gaacagtatt atcgaaatac
     2341 ccctgtagat cagcgctggg gaatcaaatt tttttgctta ggaatcggtg gtttttttgt
     2401 atacgatttt tttcttttca gtgatgcttt attgttaggt aaagtaaata ctgatatttg
     2461 ggatgctcgt ggctgggtca acttgctcac tgtgccatta gttgggttat ctgcggcaag
     2521 gaatccgaag tggtcagtcg gcatttctct atctcgccaa attctttttt actcatcaac
     2581 cttatttggt gcggctgtat atttactcgc aatggcggga gttggttatt acataagact
     2641 ctccggagga acgtggggaa ctgttctgca gcttactttt ttgtttggtg caatagtatt
     2701 acttttggtt attttatctt caggaacgac gcgttcctgg cttaaagtat ttatcagtaa
     2761 aaatttttat agctacaatt atgattatcg tgaggagtgg ttgcgtttta cacgcacctt
     2821 atcggaggag tcgttagaat taagaactag ggtaattaaa tcactcgcgc aattagttga
     2881 gagccctgct ggaatacttt ggtataaatc agagcaagga gaatttaagt taacagcgag
     2941 ttggaatttt tcacttaaaa ctcaagatat tgctgatcac agtaatgatg aattttgcca
     3001 atttcttaac caaaaaagat gggttatcga cttaggcgag tatcgattac atccacaaaa
     3061 atattcaggc ttggcgctgc catcatgggt tttagcaaat gagaaacttc gattaattat
     3121 tccccttatt ctcaataatg agttggttgg gttcattgcg ctggctgaac cgagaagtat
     3181 aattaaattg aattgggaag ttagggatct gctatgggtt gctagtacac aagcaaccag
     3241 ttatttagtt caatatgaag ttacaacggc gttgtcctta gcgcgacaat ttgaatcttt
     3301 taatcgtatg tctacttttg tagttcatga tataaaaaat ttaattttcc agttatcact
     3361 tttactcacg aatgctgaga agcataaaga gaatcccgaa tttcagaaag atatgatgga
     3421 aacagtgagt ttctcagtaa ataaaatgca acgcttgttg gaaaaattaa atagcgatcg
     3481 tgaaacagta aaaacagaat gcgtgtttct ggatcaattg ctgagacaaa tagtacagcg
     3541 aaaattaatt tgtgagccac aaccttcgtt agatatatcg gatgaaaagc ttatggtaaa
     3601 agctgatcta acacggcttg agcgagttat tgggcatcta attcaaaatg caatcgaagc
     3661 aacatctaag actgggtttg taaaaattaa actttctaaa aacgaagaca aagcgctaat
     3721 taaaattgaa gacaacgggc atggtatgag tgaagcgttt atcaaaaata aactattccg
     3781 accttttgaa acaactaaaa cagcgggtat gggaattggt gtttttgaga gcaaagagta
     3841 tattagtgag cttggagggc agttggaagt ttctagtcgt gaatttattg gaaccatttt
     3901 tttgattact ttaccgctta tggaaaatca aaagggaatt tgatctaatt ttttgctttt
     3961 tatcagctaa ttactcgata gcgccatttg ctaacatgga cagatgtcca cgaggtattc
     4021 tggtttgctg agctgaaacg tataatattt cttacgaata atcgcttgcc tcagcttatc
     4081 gctaatattc ccgtataagt tattttggta aaaggttata gagtaaagag gctatagaga
     4141 gaacatgaaa acaagtgatg acaaaagaaa gttacttgtg attgaagatg atccaggtct
     4201 tcagaagcaa ttacgttgga gttttgataa ctgcgaagtt tttgtggctg gtgatagaga
     4261 aagtgcactc gtacaagttc gacgtcatga gccttctgtg gttactatgg atttagggtt
     4321 gccacctgat cctgatgggg cttcagaagg acttgctact ctaaagcaaa tactcgcatt
     4381 agcgcctaat actaagataa ttgtattaac gggaaaccag aatcgagaga acgcacttaa
     4441 ggcgattgaa ctaggtgcct atgatttcca tcagaaacct tttgattcaa aaatgctaag
     4501 cttaattatc gatagggctt tctacttgca tgcgcttcaa cgtgagaatc gttcactatt
     4561 attgtctcat tcagattcac ctatctcagg aattgtaact cgtgatccta atatgatcag
     4621 aatgtgtcgc aatatcgaga gggttgcgcc ttctgatgtc acagtcatgt tactcggaga
     4681 gagtggtaca ggtaaagaag ttctagctaa agcgctgcat caattgagca ctagaaaaga
     4741 agggcgtttt gttgcgatta attgcgctgc tattcctgaa agcttattag aaagtgagct
     4801 gttcggtcat gaaaagggtg cttttaccgg tgcaattaag cagacattag gtaaaattga
     4861 actggcacaa ggcggtacct tcttccttga tgaagtgggt gatttacccc tatcattaca
     4921 agcgaaattg ctgagatttc tccaagaaag agttatcgaa cgaattggtg ggcgcgaaga
     4981 aataccagtc gatgttcgag ttgtttgtgc tacccatcag aatcttaaga aattgatcga
     5041 agaagggcgt ttccgagagg atttgttcta tcgattgggc gagatttcca tcaaaattcc
     5101 acctttacgc gagaggttag gagatgcagt actgctggct catcattttc gaaataaatt
     5161 tgcgactaaa gagggtcgtc attcaatgag tttttcccaa gaagcattat cagctataga
     5221 gtcttacagt tggccaggaa acattcgtga aatggaaaac tgtattaaac gcgctgttat
     5281 tatggcagag ggcccacaaa ttgcattgga tgatctagga attcaggagt ctgctaaacc
     5341 tcaagagccg cttaatttgc gtgagattcg tgaaaaagaa gaatgtaagg cggttgtgag
     5401 agcactagcg aaagtggatg gaaatattgc tagggcagct gagttattag gcgttagtcg
     5461 acctaccttg tatgacttaa tgaatcgaca tggacttaaa tagcaattct agaaagacga
     5521 gaatctcttg agatctattc atttaatgat ttattatatt cgcttatgcc gcatttattc
     5581 catgaactga tttataaagc cgctaactgc ggtgctaaat cggaagcgct tattagtcaa
     5641 cagcatcggc tggactattt agaattagca gagcaaactc agaaactagc aactgggttt
     5701 attaaaattg ggattgcacg tggtgaacgg ctggctgtat atcttgagaa aagagttgaa
     5761 ggagttaccg cattattcgc agcttctgca gcaggtggtg tattcgttcc aatcaatcct
     5821 ctgctcaagg cagagcaagt tagctatatc cttagtgatt gtaatgtaag gattctagtt
     5881 acatcaataa gtcgttttaa tttgctgaag tcagttttaa caaattgcca tgatttgcat
     5941 acgattatcg ttatagacga taacgtggag ttacctagcg tcaaggggct aaaccttatt
     6001 cgttggcaag aagttatagc tgaaaaagca tcttgctata cacatcgcac gatcgatagt
     6061 gacatggctg ctattctcta tacgtcagga agtacaggga agccaaaagg tgtagttctc
     6121 tctcaccgta atcttgtcac gggtgcaaaa agtgtttcta cttatttaaa aaataactca
     6181 gatgaccgga ttttatcagt attaccgcta agctttgatt atggattaag ccagttaaca
     6241 actgcatttt acgtgggtgc gacttgtgta ttgatgaatt acttgctacc tcgtgacatt
     6301 atggatgaag taatcaaaga ggatattact ggcctggcag cagttccccc cctgtggata
     6361 cagctttccc aactaaaatg ggaaggcttg acatcactgc gttatattac caactcgggt
     6421 ggcgcaatgc ctaaagcgac tttagatcaa cttcgtggcc tttttccaaa aacaagtatt
     6481 ttcctgatgt atggcttaac agaagcattt cgctcgactt atttgccccc tgaagagatt
     6541 aataaacgtc ctgattcgat cgggaaggct atacccaatg tagaggtttt agtattacga
     6601 gaagatggat cacaatgtgc gccgggtgaa ccgggtgaac ttgtacatcg aggtccgctt
     6661 gtttcgatgg gctattggaa tgatgtggaa aagacatcgg cttgttttag acaattgtcg
     6721 cctcgtcaaa gtggtttaac tattcctgaa attgctgtgt ggtcaggaga cacagtgcga
     6781 acggatgagg aagggttttt gtatttcatt gggcgtcgcg atgatatgat taaaacttcg
     6841 ggttatcgcg ttagtcctac ggaaatagag gaaattattt attcaaccga attagttgct
     6901 gaggttgctg cggtaggcgt cccacatcca gtattggggc aggccattgt ggtagtagtc
     6961 gttccaaggc aagacgtaaa tcttaatgct gatgttttat tgtccgcttg caagcaagcg
     7021 cttcctgcct atatgttacc tgcgctagtc gatataagaa aacatagctt acttcgtaat
     7081 cctaatggga agattgaccg taaaagtcta gctaatgaac tccaacattc atttataaat
     7141 taagtatgga ctagcgatga caaataatcg acctaagcat gcgtctttcg atcagtttcc
     7201 gatacgcgat cattgcattc agatcggagg tatgtctctt aatcgtttaa ctcagcgtgt
     7261 tgggagtact ccttattttg cttatgaccg gaaactgatt actgagcgta ttttgttttt
     7321 gcgccagtgc ctacccaata aaataaaatt acattatgca atgaaagcga atccaatgcc
     7381 ggcggtcgtt cagcatattg caggtttggt tgatggtatt gatgtcgctt cattgggaga
     7441 gattaaggta gcactagata cagggatgtt accgagtaaa ataagttttg ctgggcctgg
     7501 aaaaaatgaa catgagctct cctgcgcaat tgctgctggt attgtgatta atattgaatc
     7561 ggagcttgag ttagagcgaa ttgtagctct tgctgagcgc ttaggaataa cgcctaaagt
     7621 agccgttcgc attaatcctg atttcgaact gaaatcctca ggaatgaaaa tgggaggagg
     7681 gcctaaacag tttggaattg atgctgaacg tgccccaatt gtacttcgtc gaattgttga
     7741 attcggtata gtatttgaag ggcttcatat ttttagcggt tcacagaatc ttaaagtaga
     7801 agcaatccaa gatgcgcatg aaaaaacact tcaactagca gttaggcttg cgaaagaatc
     7861 ttcagtttct atcccttctc ttaacatggg tggagggttt ggtatacctt attttcctgg
     7921 ggaatcgccg ttgaaccttg ttgctgtggg tgagaatcta cagcatttgc tgaacaaaat
     7981 agagtcagaa cttttagata cacagtttgt gattgagctg ggtcgatata ttgttgccga
     8041 ggcagggata tatgtatgtc gggtgttaga taaaaaaaat tcgaggggac aattattttt
     8101 aattaccgat ggtggcatgc atcatcatct agcagcttca ggtaattttg gtcaagttat
     8161 tcgaaaaaat tacccagtca cagttggaac taaggcggaa accgcggaga ctgaggtggt
     8221 atcagttgtt ggtccattat gtactccgct tgatttgttg gcagatcaaa tggaattacc
     8281 caaagcagag attggtgatc tgatagtggt atttcaatca ggtgcttatg gtttgacagc
     8341 gagccctata tcatttctca gtcaccccat acccttagag gttctggttt agtctactta
     8401 cttctctaga taagctcagg cttaaatttc tataactata aaaaggaatc gatatgagtg
     8461 gagtgtgtgg ttgggttggt tatgggcaat cagttgttga gaatcaacag attagtgaat
     8521 cgatggctca agtacttacc cggtttgatc acagtcgctc gcagatttat gtcgatgaga
     8581 actcatctct tgcaatcgca gcaagcgaac aacaacttca ttactcccat aaaaatgaca
     8641 ttatcgttgg aatatgggga catgcaaaat ttaataatga tgagctgaat caactcattc
     8701 tctctcaggg aatcgctggt gttttacttg atcagtgggt gaacaatggc ccaagtgtta
     8761 ttgaaagcct actcggggaa tttatctgtt gtcttgtcaa tgctaaatcc catgaagtat
     8821 ttatagccgt tgatcgaatg ggaactcgcc cactttacta tcaaattcta gaaaatggtc
     8881 tactatttgg tacttcgctt gatgcgatga tgaggcaccc actcgcaaaa gacgaaatta
     8941 gcaatcaggg tatttatgac tatatttatt ttcatatggt gcccggtcct aatacaattt
     9001 accgtgaacc gagacgctta cttcctggtg aatatattta ctataagcat gggacgctta
     9061 cggttgaacg ttattggaat gcgcaattta gagaaaatga ggcacagtcg tttagtgaat
     9121 taaaacaaag ctttatttcg ctgctgcata cgagtatgga ggatgcaatt gcaggttacc
     9181 agcatgtggg cgcattcctt agtggtggaa cggatagttc gacaatcgca ggtatgctcc
     9241 aagaagttac gggaaaacct gctaaaacat tttctatcgg ctttgatgcc ccgggttatg
     9301 atgaaatgga ttatgcacgc attgctgccc ggcatttttc gacgaatcat catgaatatt
     9361 atgtgacacc agaagatgtt gcatcggcta taccacaggt tgcagctatt tttgatcaac
     9421 cttttgggaa tgcatctgct atacccagtt tttattgtgc tcagatggca aagaaagatg
     9481 ggattgatct gttattaggt ggtgatggtg gtgatgagct ttttgggggt aatgttcgct
     9541 atgctaagca atacgttttt tctttatatg aaagaattcc tagcgttctt cgggaaaaaa
     9601 taattacacc atttgcgcta tcaacccttg ctggatcgaa tataagctta tttcggaaaa
     9661 ttcatagcta catttctcaa gcatccatac cgatgccaca tcgtatggag acctataatt
     9721 tactcattcg ttatggttat caagatgttt ttaccgaaga ttttttatca aatattgata
     9781 taaacgagcc tgcctcacaa attaatcaaa cctatcataa agctgagaca accacactta
     9841 tcaatcgtat gctagcttat gactggaaat atacattaac ggataatgat ttaccaaaag
     9901 tcactaaagc ttgtgaatta gcggcagtag atgttgccta tccttttttg aatagcgaaa
     9961 tggtttcttt ttctgagaga cttagacctg aacttaagtt aaagaaaacg aaactacgtt
    10021 atttctttaa ggaagcacta cgtgatttcc ttccgaaaga gattattact aaacaaaagc
    10081 atggattcgg attgccattt ggaatttggc tccaagaaaa taaattactt caagcgcaag
    10141 ctgcagactg tttgacagat ctaaaaaaac gtcatatttt ccgcgtcaat tttatagaca
    10201 aaattttaag tcagtatctg catcagcatg cgagttatca cggaacaatg gtttggttgc
    10261 ttatgatgtt agaatgctgg tataagcaac gttctagata atcgtctact tctgctttat
    10321 tctacctatt aatctgcgca tgtatctatc aggctttaca tcccagcgcg actttataat
    10381 ctagcgtaat tcacttctgt aattccatat cgataaaact tctttgcgta taaagtatgt
    10441 acgtttggag gttttcatga tatttttcaa ctaatcaaag acatatacta acaggttagt
    10501 atgttatgta attattgtta tttaatctaa agaattattt agtttaagaa ggcagaagct
    10561 cactttgcta ataataatta attgaaataa aggtaaacct agaatcaatt ccaaacttta
    10621 ggtctactaa tgattagtac aatctagttt ctttagatta cattaaccca ttaatttggg
    10681 aaggattatc atgagcgata ttgccgacgc ttttcatgaa tattttgaaa ttgtggtagc
    10741 ggatacccta gaattaaaaa aagaagtgta tcgtctgcgt taccgaatca tgtgtttaaa
    10801 tacgggaatt tttgatacta cgctatatcc agatgagcta gaaaaagatg aattcgatga
    10861 ccattcaatt catattttgc ttcgtcataa accttctaac gcttttatgg gcacagcgcg
    10921 cttaatcttg tctgaccctt ctccattagc aaatcgagct ttcccagttg aactgcatac
    10981 acagttttat cccgcattct ctaatatgaa tatcattcgc gaacatacgg tcgagatttc
    11041 tcggtttgcg attcatagcg atttctttag aaggaaagat gattttcgtt tcagcgttga
    11101 caaagctgca agccaaggta ttcgaaaaaa tgaacgcaga agatttccac atcccatgct
    11161 agggctcgct gttggcatca ttcagctatg tgctaagcat gatttctttt tctggttttc
    11221 tgccatggat ccggctttaa atcgattgct gggattttat ggaatgcaat taaatccaat
    11281 tggtccaatt gttgattatt atggtccacg ccgaccttat catgtctgtg ttattgatgt
    11341 tttagaacga atgtatgtca actacccaga ggtatgggaa cttgtaaccg acaatgggca
    11401 gatttggcct tctgatctac aatcagttcg agagcggaat cagcatattt tgttagcgag
    11461 taatgaaatg gtgacttgtt aaggttctat agcctatatg gtagcgagaa accgttttaa
    11521 ttactgctat cgaatcgtct cgctattctt ttttgttttt ggcgtgtcgt attttgcaac
    11581 acacacttac gccaatgatt ccgtcgaagc agtaattcac ccgagtgtcg aattaaagag
    11641 ttattcaaga aattctttac gcgctgcgtt tggaatgcgc ctaaaaacct ggcctaacgg
    11701 catgccagtc agggtttttg ttatgcctga tgatgctgtg ttgcatacca tcttcacaaa
    11761 agaaaagctc aatatttttc cctatcagct tagagctggg tgggataaat taattttctc
    11821 aggaacagga caggctcctt tcttagtaag aactgaagaa gaaatgcgtg aacgtatatc
    11881 cagtactcct ggcgcgattg gttatttaaa gagagctaac atcgatgaaa gcatacatgt
    11941 actcaacatt gaataaacag tactcaatac tcaattatac aatgatgtgt agactcgctt
    12001 ccgtagtcct attgcttatt attatgatgc aggagggtat tgcacaggat gtaaaaggaa
    12061 tccgagtaca tggttttctg agccaaggct atttgaaatc aacaggtaac aatgacgtat
    12121 ttgggaatag cagcggaaat ggcagttttg attttagaga aataggtgct aacgcttctt
    12181 atcggccatt gcctaactta caattttcag ggcagttgtt atcgcgaagt gcgggtgagg
    12241 atagtaaagg tggtattcgt attgactacg gttttgttga ttacactgca ataagtacgg
    12301 aatcaatgga ttttggtata aggttaggta gaacgaaaaa tccattagga ttttataacg
    12361 atacgcgcga tgtaccgttt acgcgaccca gcattttact gccgcagtcg atctatttcg
    12421 atcgcactcg aaatttaggg ctggcatccg atggcgctca gatttatggt gaatccagga
    12481 atagttgggg agatttgaat gcacaatttg gggttgtttt tccgcaagcg ggggatcgaa
    12541 gtaccgagtt tgctattctg caggcagatc gtcctggagg catcacgccg cacttgtctt
    12601 acattggtcg cttgatctat gaatacgatg gtggaagagt gcgacttgct ttgagcggcg
    12661 ctcaggttaa tactggttat gacgctgctc aggtagaccc tttttcagga ttaggatctt
    12721 tccagtttac tccgattata ttttcggcgc agtacaatac ggaacgttgg agtttaactt
    12781 cagaatatgc aattcgccat atcgagcggt atgattttgc caacagatct gcacgcgatc
    12841 taaacctcac aggggagagc tattattttc agggaatcta ccgtttctcg ccgaactggg
    12901 aaggtgtgct gcgctatgat gcctactatg cggatcgcag tgatcgagat ggaaaggatt
    12961 ttgctaaatt catacaaata caggctcctg attttcaaga taattttcct tcctatagcc
    13021 gttttgccaa agatatcacg gtagggttac gttggaatgt gacaccatcg atcatgcttc
    13081 gtgctgaata tcattatatc aatggcacag gctggatttc ttcgttggat aatcgagcgc
    13141 caaatactac tttacaacga ttcctgcctg ctattaccga tcaacattgg aatttgtttg
    13201 cgttccaagc ttcatatcgt ttctagtagt caatattatg aagctcagtc atttggtgtc
    13261 actcactcgc ctcgacaggg agatccataa gacattatca aaattcttta gcttgaaatg
    13321 gaaaatttca ttgctaagta gtttgatttt gctgattgta gtcacgatgt ttagcgtgat
    13381 taattatcga agcctcattg ataacgttga ttatcagagc agtttgcaat accaacgcta
    13441 tgttaccgaa gttgagaatt taatcgagca aacgtcgaat aattctcgcc aattaattag
    13501 actgattcct ttcttgaaag aaatggatac tgcattagtg ctgcaagata gcgctcaaat
    13561 tacccaagcc tttgagcagc attggtcctt atttcagtta cataatggca tcgagacaat
    13621 tcggttttat gatcagtcag atcaactgtt ggttggttgg gataactttg aagaggattc
    13681 gcagcgtgat gccctcatat tagagcaggt ccatgatgtc aattcacaag aattaccggc
    13741 gagtttattg atttgtatcg aaatgtgttt gcaatatacc atcgagccct tattgattga
    13801 tggagttcgc gccggagcga tggtcatcgg cgcttcatta gcagatgttt ttctgggatt
    13861 taaacgaaca tttggatccg atattggatt gctcttgaaa atgaaggcag atggcgtaga
    13921 tatacaacag aatgatgtcg agttttcaaa ttggggcgtg cgcttagctg cactgacgaa
    13981 tagggaaaag aatttagaga tactccatca aatagcccaa tcacatccta ctattcagga
    14041 tcttagggta ggaattcata ctgaatggga taatcgtcat caccgaatca agctactttc
    14101 gttgcgaggt gtcaatgtat tggatcgtgc ttatttggtt gtacttacag atattacgcc
    14161 aacgattctt tccattcgag attctacctg gcggattatc attattggat tagtgggatt
    14221 actggtatcc gaactcatat tatttgcaat cctttcatta ccccttacga gactaaagca
    14281 tattgtattt acactacccc tcttggcggg tggaagcttt gggatttttc gtgctacgct
    14341 agcttctgtt agccggaaac ggtggcttaa agatgaagta gatcttctat atgaagcagc
    14401 cgtggcgtta tcccataagc ttgagaaatt agagaataag gtcgcgtatc gtaccaaatt
    14461 gcttgtccaa caaagagatg agcttagtaa agaaaaagat ttcattgctc acctgttgga
    14521 taccgcacaa gttatcgttc taacccaaaa tgttagcggg aagatcctca tgttgaatgc
    14581 gtatggtgaa ctgttgatgc gctattctaa agaagaaatt cagaccaagt attttcttga
    14641 agttttagca cccgataacg atcaacaata taatcttgtc aattaccttg aagaaattcg
    14701 ccgtggacat cgtgaccagt tgcggcatga agcgatgacc ttgtgtaatg atggctcaaa
    14761 gcgccatatt gtatggctgc attcacgatt aacctggcaa tctgaaacag atccttcagt
    14821 tttatcggtt ggtttggata ttaccgaata taagcgcgtt gaaggacatc ttgcttggct
    14881 ggcggaccat gatccactga ctaatttatt taatcgacga cggtttagtg aagaattgga
    14941 gcaaatgctg agttgggcag aaagatatca gcatccaggt gctttattgt tttttgatct
    15001 cgatcgtttt aaatatatca atgataccag tggacaccaa gcgggcgatg cattattgaa
    15061 gatggtggct gagatgctgt cgcgcacgat tcggactgct gatgtaacgg gtcgcttggg
    15121 aggggacgaa tttgcagtca tcttgccaga aatttctgca ggtggagcga tcgaagttgc
    15181 aaagaaaatt ttgttgcgct tgagtgaaac gcaattaaat gtcaatggaa gaacctacaa
    15241 aattgctgca agtgtaggga tagccctttt tcctgagcat gggaagaatg tgcacgattt
    15301 actggctgcc gctgacttag ctatgtatca agctaaagaa attgggcgag ggacgtggca
    15361 tttattttca aatgatgaca aaacgcggga gcggctatat acactggttc attggaaaga
    15421 aaaaatcgag tatgctaatt tatacgatca ttatttactc tatttccaac cgatcatgtc
    15481 attgcatgat cgagcgatta cgcattatga agtgttactg cgtatgcatg acaatgatgg
    15541 cacaattcat tcacccgctg catttatcgc agccgctgag cataccggac tgatccatgc
    15601 aattgatcac atggttttac ggaaagcgat tacccaggtc gccaaaataa atagtaaatc
    15661 gactaaaccg attcgattct ctatcaatct ctcggcccat gcattcaacg acccggaatt
    15721 attaccggtg attcggcatg aattaacttc atcgggtgta gatcccacgt tactattttt
    15781 tgaaataaca gaaacagcgg cgttagataa cttacctgga acgagaaatc tacttcgcga
    15841 gataaaaaaa ttgggttgtg ggtttgtgct tgatgatttt ggcgttggat tttcttcgtt
    15901 ctattattta cgagagctac cggtggatgc cgtgaaaatt gatggttctt ttattcggaa
    15961 tttagcgaat aatccagatg accagattct agtgagagcc ctatgtagtg ttgcaaaggg
    16021 ttttggaaag aaaattactg ctgagtttgt agaaagtgaa gaaattttaa tgctgctggg
    16081 aaaaatggat gtggactatg cgcaaggcta tcatgttgga aagccagcgg ctgctgagga
    16141 tattttccga ttgttcgagg aaaacgagat aagttagacc tggctgctaa tcattttctt
    16201 ttcgaaagaa agcgatgaat ccacccagtg ataaacataa tccaataatc gttagaacta
    16261 aagtattacc atactgaaca taaggtgtag cgcctttaaa tccctgagct gtaccatgta
    16321 gcccttgtgt tacaaacatt tcgagttctt gcacgatctg gccgcgttcg ttgatgatcg
    16381 cggttactcc tgtattggtt gcacgaagca tataacgccc tgtctccaat gcgcgcattt
    16441 gagaaatttg taagtgttgt cgtgggccaa tcgagcgacc aaaccaggca tcattactga
    16501 cattcactaa tatcgaagct tgtggaagtt gtaaaatgat ttcctcaccg aatacatctt
    16561 catagcaaat attaatggct attttttgcc cagccaaatc taatggttgt tgatcgagcg
    16621 aaccacgaga gaaatcggat aaaggaatat ttaatacctg aatgatccaa ccaaacagcg
    16681 gtttaaaagg aataaattcc ccaaaaggga ctaaatgatg tttccggtac cgttgttcag
    16741 gagaactacc catgctaaac atcgagttgt aatattcatt acgatcgatc gaatggggca
    16801 ccgtttctgc aaagccaatt aaaacatcgg cttgattttg ctttgcatga tcactcaata
    16861 gcgttaaata atccgtaggc acatcttcat aaaataatgg aattgatatt tcgggggtga
    16921 cgatcaagcg actgttgctt tcaagtacca agtctgcata agtattcatc gtgctaacaa
    16981 ggtgatcttc tcgccatttg agatcttgcg agatattacc ttgcagtaag ctaacggtta
    17041 tcgggctacc ttcaggatcg acccactgat aatgttgtaa tccatagcca ctgacccata
    17101 ttccacccaa tatccataga taatggttta gattcttttg ttttggccaa gcgatatata
    17161 aagcaatgct gaataccaat aataacgaaa taccgtaaac gccaaggata ggcgcataac
    17221 caattaaggg gcttgtgggg acttgtgaat aacctagagt caaccaagga aatcctgtga
    17281 atagcgtccc tcttagccat tcagacatga cccagcacgc tgaaacacag gctgcccaag
    17341 cgctaaaatg aatgtgtcgg atttttgtag caaaccaacc cgttaatgca gggaagagcg
    17401 ataaataggc gcaaagtaac agcagcgcta cgaccgcgat cggtaagggc atgcccccaa
    17461 agtcatgcag gctaacgtaa agccaggtta caccagctcc aaataatccc ataccaaacg
    17521 agaaaccgat gactgctgaa tgccaggatt gagtgcttga acgccataga tgaaataata
    17581 gtaaaatagt aatgactggc actgggaaat aataaaaagg tgcaaatccc aagacggtca
    17641 gcgtaccgag aatgagtgcc agaaagcttt ttgattttag gtcgatttct ttagatgaat
    17701 acacggaatg attatgaata ttgttgaact ggctcgagat agcatcgttt aattctgcaa
    17761 tttttctgca atctcaagaa tgagtttata gggcaagtaa agtaatagta tgacagctaa
    17821 ggtataaata aaatatagca gtggattttc ttttacccta ccaagtagcg tttcatgttt
    17881 accttctttt tttaattgag catattcagc tcgcacgttc aaagcgcgtt gcgtttggta
    17941 agcatcaaat aattgtttcg cttcaccgaa ttgcccatca tgggttatcc aaattgcagg
    18001 catggagata ccccaatttc cggcagtagt ttcataataa tcaatttgat ggtctaccag
    18061 taattgacgg acatcatccg cttcatcatc tgggacattt ttgagtttaa acagtagttt
    18121 tgacatcgct tagcattatg atcattacaa tgcgatgcta gagtgaagct ccgtttaatg
    18181 tcaagtcagt cttatctaaa gaaagcgcta tccccaccag tgaagcgggg gtagcatgca
    18241 agtcatcgta aatattagaa accgatccct aaatatccac ctagtgttag accatttagc
    18301 cggataccat ccaagtgacc gcccatcaaa tggtagcgcg catcaacacc taacacgata
    18361 tttttccata gtttgtaatc cgcgccggct gcaaacataa cagcaggctg gaagaaagtg
    18421 atggtttcgg agggagggct gatgatatga attgctaaac ctgccggaat gatccacggt
    18481 cttaaccggt aaccttctaa gaatttgatt ttaggtgctg cggatacagt tacggtacta
    18541 gatgttacct ggcgtggatt caaatcacca ccggcaagct gagtcggctc attagccagc
    18601 acatttccca atacggttcc gaattgttta tattcaaaca tcaattccgc gatgagttca
    18661 gttttaggaa tgagtcccca tgtgtctttg gttaagccaa agtcaaatcc agcgccaaca
    18721 taccaagcat tctggggcgc ttgttcttgt gctccgaccg gtgcaacact gctttgcacc
    18781 gaaataccat tatggtgatg atcttgtcgt gcccaaccgc cacggaaaaa aatcatattt
    18841 cgtggtgatt cttccagaat cgctttcttg atcacttcct tctcggattc tattttttgt
    18901 tgaacgatta ccctttcttc tttaatcgct tgtttgcttt cgataacttg ttgttcaact
    18961 cgtgacgagt ccgtgcggat ttggctcaac tcatcttgga tagagattaa tcgttttttt
    19021 agttctagaa cctcttgctc taatgctttg gcccgatcgt gtgccgctac cggtgatgta
    19081 aataggacta agccaaaact gaacaccata ctgatgatta agaaaaaatt gaattcttgg
    19141 tcgtagcgtt gttgctgcat tttttctcct ttgttaataa tgattaatgt tttttactta
    19201 taactccctc agctcagcag aaaaaatcaa taatctggat attaaatctg ttgaggttaa
    19261 tcaaaggctc agttactcca gcatcgagat ctctcggttt atggtattaa acgtttttcc
    19321 aggaacaaca cgatgctatg atggtacttg ctgtgacaaa aaactgagcg atgcccatct
    19381 taggggtaag ggtataaaga aatcgtgaat gaatggatgg ataaagtggg tgaaatgaat
    19441 aaatcgatat tgataacagg ttgttcaacc ggcattggtt acgtgactgc ccataaactt
    19501 cgtgaacgcg gctaccgtgt tttcgcgaca gcgcgtcgtg ctgaaagtgt agctatgttg
    19561 caagcggaag gttttgaaag cctgcagctt gatttagatg attcaaactc gattcaacag
    19621 gcgtttgcag aaatcatgca acgcacagga aatgaattat ttgcattatt taataacggg
    19681 gcctatggaa taccgggggc tgtagaagat ttaagccggg ctgccttaag agcgcagttt
    19741 gaaacgaatg tgatgggttg gctggaattg accaatttgg tgttgccaat catgcgcaac
    19801 caggggtatg ggcgtatcat tcagaatagt tcagtgctgg gatttgcagc gatgccattt
    19861 cggggtgcct ataacgcttc gaagtttgcg atcgaaggac tgaccgatac attgcgattg
    19921 gaattgatgg gcactcaaat ctatgtttgt ttaatcgagc cgggccctat ctccacgcaa
    19981 tttcgggcaa atgctttaaa agcgctacaa aaatttatcc cagtcgagca aagtgtgcat
    20041 cgtgaaaaat accaagcagt gctcagtcga ttaaataaac cagggcctgc tgttcccttt
    20101 acacaacccc ctgaagcagt agcgaaacgt gttgtagaag cattggaagc aaaagtgccg
    20161 cagcctcgct attacgtgac gtttcctact tatttatttg gttttcttaa gcgagtgctc
    20221 agcatacggt tactcgatcg cattcttgcc aaagcgggga cacagaattg atcatttgaa
    20281 ataaatgtaa aaaaaaagca ccttacttgt taaggtaagg tgcgaaggct tcgtcacaaa
    20341 atcacagctt gtcacaatga ccttgttaga aacaagagaa gaaaaataca actaacttac
    20401 actttcacta actactacac caactaacaa caaccaacaa tcaactacaa cagagacttt
    20461 aatcttatat tcttatgtat cgattaatta atctgcctat aaaactttaa aaaccatact
    20521 actgcttaga aaccaattcc aagatatgca ccggcagtta aaccactgac attgactcca
    20581 tcggttttac caaaggtttc atggtaacgc gcatcagcac ctaagaagaa ttctttccaa
    20641 atgcggtaat caaaccctgc gccaaacatc attcccggcg ctaaataagt aactgactcg
    20701 gtagtcggac
//
LOCUS       DAOQKQ010000026        20077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_1182_length_20077_cov_5.914294, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000026 DAOQKQ010000000
VERSION     DAOQKQ010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 20077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20077
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1182_length_20077_cov_5.914294"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PKZ49_02915"
     CDS             complement(<1..212)
                     /locus_tag="PKZ49_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HND35542.1"
                     /translation="MSEAIQQNNESRSKTLPAIEPLTTLEYGGNKITILGTAHVSQAS
                     ADKVKELIATGQYDAVAVELCPSRYKA"
     gene            complement(301..1680)
                     /locus_tag="PKZ49_02920"
     CDS             complement(301..1680)
                     /locus_tag="PKZ49_02920"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HND35543.1"
                     /translation="MFISVLDLFKVGIGPSSSHTMGPMRAAHDFLSRIDEISSSFSRQ
                     SVYSVRCILKGSLAYTGKGHATDRAVTLGLHNYLPEELAKEDVNALVTRLELEDTLVL
                     KNGVKLKFKPDEDIVFDKGEPLPQHPNGMIFTVVDENKKIWLTETYFSIGGGFINTLA
                     EIDQLNAPLKMETAASCPYPFDSARSMLRMSRESTLSIAEMKKANELQKITADELNQG
                     LDKIWSAMQQCVENGLKAEGRLPGGLKVERRAKKLYNQLNQDPIKATLNDWLCAYAMA
                     VNEENAAGHLVVTAPTNGAAGVIPAVLYYMTRHENGSIENVRDFLLTAAAIGGIIKHR
                     SSISGAEVGCQGEVGSAAAMAAAGLCAAQGGTAQQIENAAEIALEHHLGMTCDPVGGL
                     VQVPCIERNGFGAIKAHTAAALAKRGTGEHFMPLDSCIAAMKQTGLEMSHKYKETSLG
                     GLAVSVTEC"
     gene            complement(2168..2527)
                     /locus_tag="PKZ49_02925"
     CDS             complement(2168..2527)
                     /locus_tag="PKZ49_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HND35544.1"
                     /translation="MVSTNPILISITLLIISNIFMTFAWYAHLKELNSSPWFIAALIS
                     WLVAFFEYLFQVPANRIGYTVLSVGHLKIMQEVITLSIFVPFAIFYLKEPLKLDYLWA
                     GLCMVGAVYFMFRDKIN"
     gene            complement(2534..3919)
                     /gene="fumC"
                     /locus_tag="PKZ49_02930"
     CDS             complement(2534..3919)
                     /gene="fumC"
                     /locus_tag="PKZ49_02930"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HND35545.1"
                     /translation="MSNFRYEHDSMGEIQVSEGVLWGAQTQRSLLNFKISTEKMPFHL
                     IQALALVKRAAAKVNLELGLLDKTRAEAIISAANEIIAGQHSAEFPLAVWQTGSGTQT
                     NMNVNEVIANRASEILGGGRGNLRNIHPNDDVNKAQSSNDVFPTAMHIAAVQAVCNHL
                     MPALALLRNTLALKAESFKHIVKIGRTHLQDATPLTLGQEFSGYVAQLDHGMAHLKAA
                     LPHLYEIALGGTAVGTGLNTHPEFAHRVAKEIAGLTHFPFISAPNKFEALAANDALVH
                     AHGALKTLAASLMKIANDIRWLASGPRCGIGEIKIPENEPGSSIMPGKVNPTQSEAMT
                     MLCCQVMGNDVAINMGGALGNFELNVMKPLIIHNFLQSVRLLADGCISFHEHCVNGIV
                     ADTNRIDQLMRNSLMLVTALNPHIGYDKAAEIAKKAYHDNSSLKEAAVALGYLSAEQF
                     DNWVIPEKMVG"
     gene            complement(3958..4374)
                     /gene="queF"
                     /locus_tag="PKZ49_02935"
     CDS             complement(3958..4374)
                     /gene="queF"
                     /locus_tag="PKZ49_02935"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HND35546.1"
                     /translation="MLNKPSKKLEAFDNPFPNRDYRIHMEIPEFTCLCPKTGQPDFAR
                     LILDYIPDKKCVELKSLKLYVWSFRNEGVFHEAVTNQILDDLIALLKPRFIRITAKFF
                     VRGGIFTNVVVEHRKKNWKPVIPVILEAITEQSNTR"
     gene            4469..8038
                     /gene="smc"
                     /locus_tag="PKZ49_02940"
     CDS             4469..8038
                     /gene="smc"
                     /locus_tag="PKZ49_02940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HND35547.1"
                     /translation="MRLFQIKLAGFKTFVEPTTITIPGKLVGIVGPNGCGKSNIIDAI
                     RWVLGESRAGTLRGDSIQDVIFSGSVHRKPVGRASVELVFDNSLSKITNQWSSYAEIS
                     IKRVVQRDGDSAYYINNTHVRRRDIIDLFLGTGVSSRGYAIIEQGMISRIVEAKPQEL
                     RSFLEEAAGIAKYRERRHETELRLIDASANLLRIKDILQELEKQLLHVKEQAAAAQRF
                     NALQTELRQINSLLWLHSQQEAINLRTRTTAEIEQLMLKLEQETNNWQESDKNLEALR
                     VQHAAISDDHQRAQALLYAIEARLARTEQELRHIEENKQAIQLQIDEANTQLHRNQSN
                     KEQFQTELTHLSLEREKLDNLQKAHQQQHETENEKLPEFDHRFRISQDELFQIKQKHA
                     VTQEASKLFLNQLTHSEKIINQLTQKQTRLLQEKNNQIDSQQDKIYVLQTELEQLTFE
                     IESKQTSLTEIEEQLNLLEQSRKETLHTVQQVQQRLTSAKARYDALAQLQRQLENNEA
                     LTNWIIHNQLDQLPRLWLHIEIEPGWENALESILQEHLNAALVNRLDIALNWAEPAPG
                     PWCIVEECLNDKIKDNNALSIEFTNTSSKNNYPYKKPLSSLVKFTNSRIEEIVKQLLL
                     DVFTIDDIETALRFRHELAPTERFITSQGHLISQNSLFYYAPHTQYHGVLQRQREIVQ
                     FKNLIDQYQKEFLDIDEIYAEIEKSNEYCKQSRSTLQHELAQIKQAKHDLSLELIKLS
                     EFTQRIRDRNQQIDEELIDISAELETEFIQKQTIESKLAAHQSEIDQLSLQIDQLNES
                     VESAEKILITHRQLVHNLSNQWRDTSVQFKDCQNRIVQTENNINNLDQSAETYRNNIE
                     KLSQSLCLLENQDLQLHLAEQTDERHHCELSVTKFRQVLEETVQIIRNSELARMKSEQ
                     AINQYKESLNQLRLKQQEACLIENQFKEKLMESGSDVSTLLPQIEKVKPSVLKAELNR
                     INAELTSLGLVNLAAIEELQTIQSRFTFLDLQSADLENAVMTLENAIHQIDGETRERL
                     QETFDRVNLLFHELFTSVFGGGNAKLTLSGEEILDATIQLIAQPPGKKNNSIHLLSGG
                     EKALTALTLIFSLFQLNPAPFCLLDEVDAPLDDINAMRFCELVKRMANETQFLFISHN
                     KISMQMAEQLIGVTMAEQGVSRIVSVNFEDVINPVQDQEARLV"
     gene            8125..9252
                     /locus_tag="PKZ49_02945"
     CDS             8125..9252
                     /locus_tag="PKZ49_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HND35548.1"
                     /translation="MSDLQISLIIIGAIIIAGVAIFNWVQQIQYQRNIKKAFEHDHED
                     ALLKPSKFVSKEERIEPKFNQQANESELEPIVPIATEDIPTIPAHISVTPTHEQKPIH
                     IDSVINYITSITTTDLITHDKLTELLQQKFDFGKPVTWYGLNRDLQAWEEITIETLHG
                     RGFYSQLKGCLQLADRAGPVSEVNLSKFRDMVEDFASQLNVIAECPDIAQSHTNAITL
                     DKFCADVDVVLGINVVSKDGGAFVGTKIRAMAEASGFKLESEGMFRFRDENNATLFSL
                     CNYETTPFSPANMRTLTTHGITFLFDVPRVANGEKVFEQMAHLAKLFASTLNGIMVDD
                     NRVPLSDNGLKRSKQQLKNIQAAMSAHNILAGSDVALKLFV"
     gene            9283..9516
                     /locus_tag="PKZ49_02950"
     CDS             9283..9516
                     /locus_tag="PKZ49_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HND35549.1"
                     /translation="MSEIKKFSPIEVTLEANRQYYWCSCGLSKSQPFCDGSHKGTTFT
                     PLAFSVEQTKSVWLCTCKKTANKPYCDGTHNKI"
     gene            9550..10545
                     /gene="lpxK"
                     /locus_tag="PKZ49_02955"
                     /pseudo
     CDS             9550..10545
                     /gene="lpxK"
                     /locus_tag="PKZ49_02955"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
     gene            10570..10749
                     /locus_tag="PKZ49_02960"
     CDS             10570..10749
                     /locus_tag="PKZ49_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HND35550.1"
                     /translation="MDTKLLDILVCPLCKGSLIYKKVEKELICKRDRMAFPIRDGIPI
                     MLEDEARLLPPEEEV"
     gene            complement(10855..11025)
                     /locus_tag="PKZ49_02965"
     CDS             complement(10855..11025)
                     /locus_tag="PKZ49_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35551.1"
                     /translation="MRPQIITQLSDFPESIDEQIPDTRVMKVIDDTDFTDDDFEVEEI
                     EVNKVINDLLLH"
     gene            11356..12186
                     /locus_tag="PKZ49_02970"
     CDS             11356..12186
                     /locus_tag="PKZ49_02970"
                     /inference="COORDINATES: protein motif:HMM:NF022591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HND35552.1"
                     /translation="MNDQQLKILIKLNQIPQSPVSTIADNSEITESHDEEKLPFDWLK
                     ISAALLLLFLLLGVSYWLLTGSEKESAESSIFSNEDRSQSTDSNQQAPSDLESIVDAT
                     SPETARTIVVPSDLGKQTESVSNAVTSNENHAQQAMIIPSSKPESKGLKLNNSQSKEK
                     PTQTQSNYVIKAQLTTSIRKQESINSIDHIELQRGMNQTIYYSLHIRNLKGEKIFVNW
                     YYQDKQVAKIPFSINTNDWQAHSSKILTKTSLGRWRVTAMDHLGNILSEQFFSVSNRS
                     "
     gene            12234..12776
                     /locus_tag="PKZ49_02975"
     CDS             12234..12776
                     /locus_tag="PKZ49_02975"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HND35553.1"
                     /translation="MNYCSHCSAPVELRIPDGDSLPRFVCGTCNTIHYQNPKIIVGCI
                     PEWNNQILLCRRAIEPKIGLWTIPAGFMENQETLAEAAIRETNEEANANIEIGDLYAV
                     YSLPHISQVYVLYRAKLIDTNFFPGIESQETKLFHEHEIPWGDIAFKVIHDSLKRFIE
                     ERRQNSIQFYTGVITRAPSQ"
     gene            13077..14960
                     /locus_tag="PKZ49_02980"
     CDS             13077..14960
                     /locus_tag="PKZ49_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HND35554.1"
                     /translation="MSLEQLMNVSITGASKYEQKQSEVAAAVTVITRNDIRAYGWRTL
                     SEILTSLPGVFATYDFSYNYIGVRGFNVLGDFNTRILVSINGNRINDATYDQGPMGRD
                     FPLDVDLIERIEFIPGPGSAVYGQNAMLGVVNIITRKGSNVNGVELSGSYQTAEVMPQ
                     ERATLGKKFDNGVDALVSVSGVQARGENLFFDFRDAAISGVAHHMDGENIKQIFARAE
                     KGPVSFDFIYGNRKKDDPTAIYFSDPLISGQYLKDRRLNSQLQYNDNFVNDTLNVLGR
                     LFIGKYDYDSPLVYEGKKTLSTGPSFWHGAELRLVSSAITNHKLMAGVEYQNNTHIKQ
                     TFEYLESLADNITVRSSVVRTGVYLQDEWRITDTLSSTLGVRYDYNNWIGNRLSPRGA
                     LIWQATPKAAFKAIYGRAHRSPNSYERDYGDGVSQISNPGLRGETIDAAELVADYFPE
                     PNLNLRASLYAWDVYNIIALGFNPLLEASQYQQTHDKVRARGVELYFDKTWNWGARLR
                     SSFGIQDASQKGSHIPNSPQLLGKINVLVPIPLMSGLKGGYELQYFGERKTLNGPNTD
                     SYFLSNLNLMTDIRWVKGLEASLAVYNLFNEHYLHPVSDSSWHNSLLQPGRTVRFRLE
                     YRF"
     gene            15134..15601
                     /locus_tag="PKZ49_02985"
     CDS             15134..15601
                     /locus_tag="PKZ49_02985"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HND35555.1"
                     /translation="MLYSPNSFGQPEEPTEYRLKAAFLYNFASFPEPPTKIDKHSTFN
                     MCFLDYIAQRKVKDLQVTILLKSDPDKLSECHLLYISRSVASNKLESILDRISSGPIL
                     TVADSTGICQKEVVTNMGINAGKTTFKTNLTPAHTAKLELSSQLLRFAKEAYQ"
     gene            15601..18378
                     /locus_tag="PKZ49_02990"
     CDS             15601..18378
                     /locus_tag="PKZ49_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HND35556.1"
                     /translation="MNPLLPATTTIKNKLQKISLISQSTAMFLLAGLVILSSFIINLY
                     SLLETSQSTAKILAENATATLMFQDKNSAQTLLYSLNNLSRVNAAAIFSENHHQFAEY
                     PNNHHAFPHFDSLNEEKILIDAQYIKLLQPIRFNDQSLGALYLEVTMLPLYWQMLWQV
                     LITIAAATIALYVAYRLLQHLNRSVLNPLNQLSEVIEHVTSNADYKARTPKSNIVELD
                     MLANGFNNMLEVIQERDSKLANHLDHLEDEVSKRTSELVLAKEAAEAASKAKSEFLAT
                     MSHEIRTPMNGILGMTELLLNTELSKDQYRFADTVQRSGQHLLGIINDILDFSKIESG
                     HMELETVDFELVKLIEDTLIMFAQPADEKGLELAAQFIPPNIPIAIKGDPFRLKQVLV
                     NLINNAIKFTSQGEVIIRTRIIEESETQIHINICVADTGIGIPEAYHDKIFQHFSQAD
                     GSTTRQYGGTGLGLTICKRLLELMNGRIWVESSSGHGSKFWIDIKMEKCSLAAIAALP
                     NLEILHNTRVLIVDDNQTNREILELQLKSWNVKVNCAENAERALIMMSKAVEDCDPYR
                     LAILDMHMPKMDGLQLAKIIASDANLSKTRLMMLTSTYSNASQLERQNVGILRCANKP
                     VRQRELLEIILDVLQRSDKSAYSETPIKQSTSALPAKNLTQGKVLLAEDNLVNQEVAK
                     AMLAKLGFKVDIANDGQQALDFMNKQRYDIVLMDCQMPIMDGFEATTQIRKQYGQTIP
                     IIALTANATEDDRNHCIEAGMDDFLSKPYTLNQLNQVLTKWETKTQSNLVDAMSESHV
                     PKENTEIKKMPLLNPVLLDQIRSLDTSNGHELVNKILCAFLESAEKYIELLDRAFNDK
                     DIETIRKTAHTLKSSSANIGAEELSTIFKQIEAYSKNGELALAESLYSGMLAIYQQVI
                     IEVKAIINQSQ"
     gene            18375..>20077
                     /locus_tag="PKZ49_02995"
     CDS             18375..>20077
                     /locus_tag="PKZ49_02995"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HND35557.1"
                     /translation="MSRTFKILLADDDVTIRFLMQAALEQAGFEVILASNGEEAIQLF
                     NTSSPDMVMLDVEMPQMNGYQVCTYLRKQIGNELPIIMVTGMDDVQSIDHAFEVGATD
                     FIAKPINWNLINYRLLYLRRAYLNLLDLKNANARSKAIFSAIPDTMFIIDNNGHIVDT
                     QSSSDKNTFLNFQTGDDLNQRMPANITQAHLEAAGRARKYGAVEHFEYQLNGDSDREK
                     YYESRVVAIDSNEILCLVRDITERKDSENRIFRLAYFDSLTGLPNRQSFIDRLKREIK
                     RAKYIGSKLAILFLDLDGFKSINDTMGHNTGDVILQWAADRLQSSTRSSDFVSRSNTT
                     GSEIELARLGGDEFTVVIPNLTRAEDALVLAHRIRETMRRPFRLDSRDVVLTTSIGIA
                     LYPDDGEDVEALLKHADTAMYHAKTEGRDNCQFYSVTLTQEAEKRLNLENDLRNALKN
                     NEFHLLYQPQFDVERSHFKSAEALIRWNHPKQGLVSPLDFIPLAEENGLIIPIGEWVL
                     RTACSDAAHWLKNGGDLKVAVNLSPLQFKNPDLIKSVLSILTETGLPPHKLTLEVTEG
                     A"
BASE COUNT         6382 a         4010 c         3857 g         5828 t
ORIGIN      
        1 gctttgtatc gactagggca taactccact gcaaccgcat catattgtcc agtggcaatt
       61 aattccttaa ctttatctgc acttgcttgg gaaacatgtg ctgtgcctaa aatcgttatt
      121 ttgttgccac cgtattcaag tgtagtaaga ggttcaatgg caggtaatgt tttagaacga
      181 gattcattat tttgttgtat tgcttcagac atgaacaacc taattgatgg tcggaaaaat
      241 taaagggttg aaattatgaa tgactgttgg tatgagtagt cagtttgtaa aaatagctag
      301 ctagcattct gtaacactca ctgccaagcc acccaatgag gtttctttat atttgtgcga
      361 catttctaaa ccagtttgct tcattgcagc aatacagcta tctaaaggca tgaaatgttc
      421 tccagtgccc cgttttgcaa gggcagctgc tgtatgggcc ttaatagcac caaacccatt
      481 acgctcgata cagggaactt gtaccaaacc accgaccgga tcacaggtca tacctaaatg
      541 atgttcaagt gcgatctcag cggcattctc gatttgttgt gctgttccac cttgagctgc
      601 acacaatcct gctgctgcca ttgctgctgc agaacctact tctccttgac aaccgacttc
      661 agcacctgaa atcgagctgc gatgtttaat aatgcctcca atggctgcgg cagttaatag
      721 aaaatctctt acattctcaa tactgccatt ttcatggcgt gtcatgtaat acagtacggc
      781 agggatgaca ccagctgctc cattcgttgg ggcggttaca actaagtgac ccgccgcatt
      841 ttcttcatta accgccatcg cataagcaca tagccagtca ttcagcgtgg ctttgattgg
      901 gtcttgattt aattggttat atagcttttt tgcacgccgt tctactttaa gtccaccggg
      961 cagtcggcct tctgccttaa ggccattttc aacgcattgc tgcattgccg accaaatttt
     1021 atctaacccc tgattgagtt catcagcagt tatcttttgt aactcattgg cctttttcat
     1081 ttctgcaatg gaaagagtgc tttcacgaga catccttagc atacttcgcg ctgaatcaaa
     1141 tggatagggg catgatgccg cagtttccat tttaaggggc gcattgagtt gatcaatttc
     1201 tgctaaggta ttgatgaatc cacctccaat cgagaaataa gtttcggtaa gccaaatttt
     1261 cttattttca tcaacgacgg taaaaatcat tccatttgga tgctgtggaa gcggttcgcc
     1321 tttatcaaag acaatgtcct catcaggttt aaatttaagt ttgaccccat tcttcagtac
     1381 gagcgtatct tccagttcaa gccgcgttac tagcgcattg acgtcttctt tcgcaagctc
     1441 ctcaggtaga tagttatgaa gaccaagcgt aactgcacga tcggttgcat gacctttacc
     1501 cgtataagcc aaggaccctt tcaaaataca gcgaacacta taaaccgatt gacgtgagaa
     1561 tgaggagctg atttcatcaa tacgcgatag gaaatcatga gcagcccgca tcggtcccat
     1621 ggtatgagaa cttgaagggc caattccaac tttaaagaga tccaaaacac ttataaacac
     1681 gaatgtaaac cttatctatt agatagcgaa aatgaatgat taatatatgg ggagctatat
     1741 taatataaaa agaacttgtg atgtctgata aagaaagggg aatgttatct gctttacagc
     1801 actatttatt tcgaaaataa ttttatttaa cataatatac attatacgca attaagggtc
     1861 tgttatcaga gcaaatttac tctgtctagt tttatctccg atgaatcatt tgctaggcgt
     1921 tacagcatgc cgcttgacga tgttttagat ttgtgtgttc aaggtcgttt cattgaagct
     1981 tcttacagcc cttcaaagcg tcgttggttt atcccgattc ccgtgaggat gtcgagagcc
     2041 atgattaaac gttgctacaa acgcaagcca tttaaaccaa tcgattacga acgattcaaa
     2101 gaaacccgct tgctttcaag aagcctaagt aggcggcaaa acttcagcct tgtttttttt
     2161 tattaatcta attaatctta tctctaaaca tgaaataaac cgcaccaacc atgcacagtc
     2221 ccgcccataa ataatctaac ttgagcggtt ctttgagata gaaaatagca aatggtacaa
     2281 aaatactgag cgtgatgact tcttgcataa ttttcaaatg tccgacactt aaaacggtat
     2341 aaccgattcg atttgctggc acttggaaaa gatactcaaa aaaagcgacc agccaactga
     2401 ttaaagccgc aataaaccac ggtgatgaat tgagctcttt gagatgcgca taccaagcaa
     2461 aagtcatgaa aatattgcta ataatcaata aggtaatact gattaatata ggattggtac
     2521 taaccatagc tgattaaccg accatttttt caggaataac ccaattgtcg aattgttcag
     2581 cagacaaata acccagtgct accgccgcct cttttagaga actattgtcg tgataagctt
     2641 tttttgcgat ttcggctgct ttgtcatagc cgatatgagg gttcaatgcc gtcactaaca
     2701 tgagcgagtt tcgcattaat tgatcaatgc ggttagtatc agcaacaatt ccatttacac
     2761 agtgttcatg aaagctaata cagccatccg ctaacaaccg tacactttgt agaaaattat
     2821 ggataatcag cggtttcatt acattcagct cgaagtttcc taacgcccca cccatattaa
     2881 tcgcgacgtc attacccatc acttggcaac acagcatcgt cattgcttca gattgagttg
     2941 gattgacttt tcctggcatg atggagctac ctggctcatt ttcaggaatt ttaatttcac
     3001 caattccaca acgaggacca gaagctaacc agcggatatc attggcgatt ttcatcaagg
     3061 atgcggctag cgtttttaaa gcaccgtgtg catgcacgag tgcatcgttt gcagctagcg
     3121 cttcaaattt attaggcgct gatataaaag gaaaatgagt cagacctgct atctctttag
     3181 caactcggtg agcaaattca ggatgcgtat ttaatccggt tcccactgcg gttccaccca
     3241 gtgcaatttc atataaatgc ggtaaagctg cttttaaatg cgccattcca tgatctaatt
     3301 gtgctacata tcctgaaaat tcttggccta gcgtcaacgg tgttgcatct tgcagatgtg
     3361 tacgtccgat tttgacgata tgtttaaatg attcagcttt taaagccaac gtattacgaa
     3421 gcagcgctaa cgccggcatt aagtgattac aaactgcctg aacagccgca atatgcatcg
     3481 cagttggaaa tacatcgttt gaagattgag ctttattaac atcatcgttg ggatgaatgt
     3541 tccgtaaatt tccacgccct cctcctaata tttcagaagc gcgattggca atcacctcat
     3601 tcacattcat attagtttgt gtgccggagc cggtttgcca aactgctaat ggaaattcag
     3661 cactatgctg acccgcaatg atctcatttg ctgctgaaat aattgcctct gcccgtgttt
     3721 tatcaagcaa tcccagttca agatttactt tagctgctgc tcgcttgacc aaggccaacg
     3781 cttgaatcaa gtggaaaggc attttttcag tagatatttt gaaattgagg agtgaacgtt
     3841 gtgtttgtgc tccccataac acaccttctg atacttgaat ttcacccatc gaatcatgtt
     3901 catatctgaa attactcata aataaatcga ccgttgagtt aaagtaatgt aaacgttcta
     3961 gcgtgtattc gattgttcgg taatcgcttc taatataacg ggtattactg gtttccagtt
     4021 ttttttgcgg tgttctacga ctacattggt aaaaattcca ccccgaacaa aaaactttgc
     4081 agtaatccga ataaaccgtg gttttagaag cgcgatgagg tcatcgagaa tttgattcgt
     4141 aacggcttca tgaaatacac cttcattacg aaaagaccaa acatacaatt ttaaactttt
     4201 taactcgaca cattttttat cgggaatata gtccaatatc aaacgcgcaa agtcaggttg
     4261 tcccgtttta ggacacaggc acgtaaattc aggtatctcc atatgaatac gataatcccg
     4321 atttggaaat ggattatcaa aagcttccaa ttttttggag ggtttattca gcatgcgtta
     4381 cattccatct aggattgatt aagtgaattc agataaaata gattaaaacg atttagttct
     4441 gaattatacc aattatttac cctctcaatt gcgcctcttc caaatcaaac tggctggttt
     4501 taaaacgttt gtcgaaccca caacgatcac gatcccaggc aaattagttg gtatcgttgg
     4561 accgaatggg tgtggaaaat ccaatatcat cgatgcgatc cgctgggttt taggtgaatc
     4621 acgtgctgga actttgcggg gtgattcaat ccaagatgtg attttcagcg gttccgtcca
     4681 ccgaaaacca gtcggtcggg ctagtgtcga attggttttc gataactcct taagcaaaat
     4741 cacaaatcaa tggtcgtctt atgcagaaat ttccattaag cgtgtcgtgc aaagagatgg
     4801 cgattccgcc tactacatca ataacaccca tgtaagacgt cgcgacatta ttgacctttt
     4861 cttgggaacg ggtgtgagca gtcgcggcta tgccattatt gagcaaggga tgatttcccg
     4921 cattgtggaa gctaaacctc aagaattacg atcatttcta gaagaagctg ccggtatcgc
     4981 aaaatatcgc gaacgtcgcc atgaaaccga gctacgtttg atcgatgcaa gcgctaactt
     5041 gctgcgtata aaggacattt tacaggagct tgagaagcag ctacttcatg ttaaagaaca
     5101 agctgctgct gcccaacgtt ttaatgcact gcaaacagaa ttacgtcaaa ttaacagttt
     5161 gttatggctt catagtcaac aagaagcaat caatttacgc actagaacta cagccgaaat
     5221 tgagcaatta atgcttaagc ttgaacaaga gaccaataac tggcaggaat ccgacaaaaa
     5281 ccttgaagcg ttacgggttc aacacgctgc tatcagcgat gaccatcaac gcgcgcaagc
     5341 tttgctatat gccatcgaag caaggcttgc tcgaactgaa caagaactca gacatatcga
     5401 ggagaataaa caagcgatcc aattgcaaat tgacgaagct aatacgcaac tgcatcgtaa
     5461 tcaatctaat aaagaacaat ttcagactga attaacgcac ttatcgcttg aaagagaaaa
     5521 acttgataat ttgcaaaaag cccaccagca gcagcacgaa acagaaaatg aaaaattacc
     5581 tgagtttgat catcgattcc gaatctcgca agatgaatta tttcagataa aacagaaaca
     5641 tgctgttacc caagaagcta gcaagctttt tctgaatcaa ttaacgcata gtgaaaaaat
     5701 tattaatcag ctaacacaaa agcaaacgcg gttgcttcaa gaaaaaaaca accaaattga
     5761 tagtcagcaa gataaaattt atgtgttgca aaccgagcta gagcaactca cgtttgagat
     5821 tgaatcaaaa caaaccagtt tgaccgaaat tgaggagcag ctaaatctct tagagcagtc
     5881 tagaaaagaa acacttcaca cagtgcaaca agtacaacaa cgattgacat cggcaaaagc
     5941 acgttacgat gcattagctc aattacagcg ccaacttgaa aataatgagg cgctaacaaa
     6001 ctggataatt cataatcaac tcgatcaatt accgcgatta tggctgcaca tcgaaatcga
     6061 accgggatgg gaaaatgcat tagaatcgat tttacaagaa catctcaatg ccgctttggt
     6121 aaatcgctta gatattgcac taaactgggc tgagcctgct ccaggcccat ggtgcatagt
     6181 agaagaatgt ttgaatgata agattaaaga caataacgct ttatcgattg aatttacaaa
     6241 cacttctagc aaaaacaact atccgtacaa gaaaccgttg agttcgcttg ttaaatttac
     6301 aaactcacgt atagaagaaa tcgttaaaca attactattg gatgttttta caatcgatga
     6361 cattgaaacg gctttgcgtt ttcgccatga attggcgcca actgagcgat ttattacgtc
     6421 tcaggggcat ttaatttcac aaaatagttt attttattac gcacctcata ctcagtatca
     6481 tggtgttttg caacgccaac gcgagatcgt tcaatttaaa aatttaattg accaatatca
     6541 aaaagaattt ctcgatatag atgaaatcta cgctgaaata gaaaaaagta atgaatattg
     6601 caaacaatcg cgttcaacac tgcaacatga gttagctcaa ataaagcaag cgaaacatga
     6661 tctatccctg gaattgataa agctatcaga atttacgcag cgtattcgcg atcgaaatca
     6721 acaaatcgat gaagaattga ttgatatttc agctgaatta gagactgagt ttatacaaaa
     6781 gcaaacaata gaatcaaagc tagcagcaca tcaatccgag attgaccaat tgagtttaca
     6841 aatagaccaa ctcaatgaat cagttgaatc agcggaaaaa attttgatca cgcatcgtca
     6901 gttagttcac aatttatcaa atcaatggcg agatacttca gttcaattca aggactgcca
     6961 aaatcgaatt gttcaaactg aaaataatat caacaatctt gatcaatccg ctgaaacata
     7021 ccggaataat atcgaaaaac taagtcaatc gttatgttta cttgagaacc aagatctaca
     7081 acttcacctc gctgagcaaa ccgatgagcg ccatcattgt gagttatcag ttactaagtt
     7141 ccgtcaagtt cttgaagaaa ccgttcaaat tatacgcaac tccgaattag ctcggatgaa
     7201 atcagagcaa gctatcaacc aatataagga atcactgaat caattaaggc tcaaacagca
     7261 agaagcgtgt ttaattgaaa atcaatttaa agaaaaatta atggaaagcg gttcggatgt
     7321 cagtacactt ctgcctcaaa tagaaaaagt taaaccatca gtattgaagg ctgaactcaa
     7381 tagaattaat gctgagctga catccttagg acttgtgaac cttgcagcaa tcgaagaatt
     7441 acaaacgatt caaagtcgtt tcacatttct tgatctacaa tctgcagatt tagaaaatgc
     7501 ggtaatgact ttggagaacg caatccatca aattgatgga gaaactcgag aacgcttaca
     7561 agaaacattt gatcgcgtaa acttgttatt tcatgaatta tttacttcgg tttttggtgg
     7621 aggtaatgct aaattaacgt taagtggtga agaaattctt gatgctacga tccaattaat
     7681 cgcgcaacct cccggcaaaa aaaataactc aattcatctc ttgtctggtg gcgaaaaagc
     7741 gttaacagcg cttacgctca ttttctcatt atttcaactg aacccggcac cattttgcct
     7801 tttggatgaa gtcgatgcgc cattggatga tattaatgca atgcgtttct gtgagctagt
     7861 aaagcgcatg gccaatgaaa cacaattctt atttattagc cacaataaga tttccatgca
     7921 aatggcagaa cagcttattg gcgttaccat ggcagaacaa ggggtttctc gtatcgtctc
     7981 cgtgaatttc gaggatgtga taaatcctgt acaagatcag gaagcaagac ttgtctaaaa
     8041 cttactatca gattacttat tagtggctat aattggtatt taaaaaagct taatcaagtt
     8101 taataatatt gaatggatca aagtatgagt gatttacaaa ttagcctaat cattattggc
     8161 gcgattatta ttgccggcgt tgctattttt aattgggtac aacagatcca atatcaacga
     8221 aatattaaaa aagcatttga acatgatcac gaagatgcgc tactaaagcc gagtaaattt
     8281 gtatcgaaag aagaacggat cgaaccaaaa ttcaaccagc aagcgaatga gtctgagtta
     8341 gagccaattg taccgattgc aactgaagat attcctacaa ttcctgcaca tatttctgtc
     8401 acaccaacac atgaacagaa accaattcat attgatagtg tgatcaatta catcacatca
     8461 attaccacaa ctgatttgat tactcacgat aaattaactg agttactcca acaaaaattt
     8521 gattttggta aacccgtaac atggtacggt ctgaatcgtg acttacaagc gtgggaagaa
     8581 attaccattg aaacccttca tggcagagga ttttattctc aattaaaagg atgcctccag
     8641 cttgcagacc gggcaggccc tgtgagcgag gtcaatctat ccaaatttcg cgatatggtt
     8701 gaagattttg catcacagtt aaatgttatt gccgaatgtc ctgatattgc tcaatcccat
     8761 acaaatgcga taacacttga taaattctgt gctgatgttg atgtcgtgct aggaatcaat
     8821 gttgtcagta aggatggcgg tgcctttgta ggtaccaaaa ttagagcgat ggctgaagca
     8881 tcgggtttta aacttgaatc agaaggtatg tttagattcc gggatgaaaa taatgcaaca
     8941 ttattttcac tttgtaatta tgaaacaaca cctttctccc ctgccaacat gcggacgctc
     9001 accactcacg gcatcacctt tttatttgat gtaccccgcg ttgcaaatgg cgaaaaagtt
     9061 tttgaacaaa tggcccatct tgccaaactt tttgcctcaa ccttaaatgg cattatggtt
     9121 gacgacaatc gtgtgccact gagtgataat ggtctaaagc gaagtaaaca gcaattgaaa
     9181 aatattcaag ctgcaatgtc agcccataac attctagccg gcagcgacgt tgcacttaag
     9241 ctatttgttt aacattaata actgaactaa gaaggtcatc caatgagcga aattaaaaaa
     9301 ttttctccga ttgaagtaac actcgaagcc aatcggcaat attattggtg ttcatgtggt
     9361 ttaagcaaat cgcaaccttt ttgtgatggt tcacacaaag gaactacttt tacacccctc
     9421 gccttcagcg ttgaacaaac taaatctgtc tggctttgta cctgtaaaaa aactgctaat
     9481 aaaccctatt gtgatggtac gcataataaa atttaaccag aatcaacttt taggtattta
     9541 gttaaattta tgcatgtaag ctggtctgaa ttatactggc agcgattaac accacttcat
     9601 gcagttttat ggccaattag tatactgctt gagctatcat ttaagcttaa acgctcaact
     9661 tatcgctatg gcctgctttc cccaatacga tatcccgttc cgattatcat tgttgatagc
     9721 ataacggtta atagcattga taagtcggcg ttcactgtct ggttagtaaa ttttttaaca
     9781 tctaatgggt tgcgacctgc tgttattagc tgtagctacc ctgatagcta tcaggaatcg
     9841 ccaattgaag tgacaacttc gagcgataaa gctcaagttg gagatactgc ttcattcctg
     9901 gcttttcatt gtagtaagca tcattgccct gtctggatcg gacaggatat tagtacggtt
     9961 ggattcgatt tactaaaggc tcaccctgat cggaatgtat tgatatgcaa taacgggttg
    10021 catgactatc gtttgtttcg cgatttagaa atattagttt tagatagcga taaacattat
    10081 tttggaaatg gctttacttt gccagctggc ccacttcgag ataatcttac gcgtttaaat
    10141 ttggccgatg caattgtttt aaacgaaaaa aataggcagt cttttgaaca agtcgataac
    10201 tcaaaaattt actatgtcaa aaccagtagt agcgaattta ttaatttttt tcatactagc
    10261 gatcatttta aaaaattaac tacatcgggc cagaaactac acgctattgc cagcattaaa
    10321 catgctaaac gtttttttga ccagcttgct tatttgaata ttactgcaag caaacaatcg
    10381 atactgaatg aatcacaatt taccaaacgt tatattgatt cacttaacgc agatattatc
    10441 ttgatcaaag aagaagatgc ggttaaatgt ttgagtttta ttgatgatcg tttctggatt
    10501 ttgaaagaat cgattgttgc aaatacagcg ctacaagtaa cgttattgaa caaactgaga
    10561 gaaaaattta tggatactaa attattggat attttggttt gccccttatg taagggttct
    10621 ttgatctata aaaaagtgga aaaagagctg atttgtaaac gtgaccgaat ggcgtttcct
    10681 attcgtgatg gcattccaat tatgctagag gatgaagctc gcttacttcc acctgaagaa
    10741 gaagtttaat cttcttgaaa tttcctgaaa acaactacga atctaagtcc atatcatcgg
    10801 tgaaagtaag caacttcttt gtactgtcat tggatcagac acttcatggc ttgtttaatg
    10861 taataacagg tcatttatca ccttatttac ttcaatttct tctacttcaa aatcatcgtc
    10921 agtaaaatca gtatcatcaa taactttcat cactcgagtg tccggaattt gttcatcgat
    10981 tgattccgga aaatcagata gttgagtaat aatttgtggt ctcattttaa atattctcca
    11041 tatagaattt taataatgaa gtgcaatgtg cttaccatta atctatacgg aagaattgaa
    11101 attaaccgtg agaaaaaaca caaagatttt tccaaaaaat aatttttttt attgtcatta
    11161 acaccgtatt ctcacccagt agccatggcg tttgtagatt ctttttctaa gttgacttaa
    11221 gccacacgga atttggatat aatttagtaa ttatttagcc ttcatgacca aggctcggaa
    11281 ttaatgacca ataagcatct ctctgcaaca atagcctaga atatttctta aaccatacta
    11341 tcgatatact gcaaaatgaa tgatcagcaa cttaaaatcc ttatcaagct taaccaaatt
    11401 cctcagtccc cagtatctac gattgccgat aattctgaaa taactgaaag ccatgatgaa
    11461 gaaaaactgc cctttgattg gcttaagatt tcagcagcct tattgctatt attcctgcta
    11521 ttaggagtga gttattggtt actaaccggt agcgagaagg aatcagctga atcatcgatt
    11581 ttctcaaatg aagataggag tcaatccact gatagcaatc aacaagcacc ttctgatcta
    11641 gaatcaatcg ttgacgcgac ttcacccgaa acagccagaa cgatagtggt ccccagtgat
    11701 ttagggaaac aaactgagtc cgtaagtaat gctgtcacta gcaatgaaaa tcatgctcag
    11761 caagcgatga ttattccttc atccaaaccc gagtcaaaag ggttgaaatt gaataattcg
    11821 caatccaaag aaaaaccaac gcagacgcag tcaaattacg ttataaaagc acaattgact
    11881 acatcaatcc gaaaacaaga atctattaat tcaattgatc atattgaact ccaacgagga
    11941 atgaatcaga ctatttatta ctccttgcat attcgtaatc taaaggggga aaaaatattc
    12001 gtgaattggt attaccaaga taaacaggtt gctaaaattc cgttttcaat caataccaat
    12061 gattggcaag cccactcaag taaaatttta actaaaacaa gcttgggtcg atggcgtgtt
    12121 acagcaatgg atcacttagg taatatttta tccgagcagt ttttctcggt tagcaatcgt
    12181 tcttgattca tctcatggcg tgagttttaa cctaaattac aattagcaaa cacatgaatt
    12241 attgtagcca ttgcagtgct cccgttgagt taagaattcc agatggcgat tccctacccc
    12301 gcttcgtatg cggaacctgc aacaccattc attaccaaaa tcctaaaatc attgtgggat
    12361 gtatcccaga atggaacaat caaatcctat tatgtcgacg cgcaatagaa ccaaagattg
    12421 gattatggac aattccagct ggatttatgg aaaatcaaga aacattagca gaggctgcga
    12481 ttcgtgagac caatgaagaa gccaatgcaa atattgaaat tggtgacctc tatgcagttt
    12541 atagcttgcc ccatatcagt caggtttatg ttttgtatcg ggcaaaatta atcgatacca
    12601 atttttttcc aggtatcgaa agtcaagaaa ccaaattgtt ccatgagcat gagattcctt
    12661 ggggtgatat cgccttcaaa gtcattcatg attctttaaa acgctttata gaagaacgcc
    12721 gtcaaaatag cattcagttc tatacgggcg tcatcactcg cgctccatcg caataaaatt
    12781 tatttcttaa cattgggtaa attattaccc aatccaccga tacgtttcgc agtatttcat
    12841 aggaccagta ggctcaatag cagtcacctg ctatggttac tgtgaaatgt ccctgatttt
    12901 agcgttttca attcaattca agcgcatacc tcaactgata aattagcaaa ttagtccttt
    12961 tgctacttat tggcattaat tatcactctt aagttcttat catgcgctgt atttatttaa
    13021 ctttagtttt gacttttttt tcgtttcaag tgctagcttc caagcaattt gaagatatga
    13081 gtctggaaca attaatgaat gtcagtatta caggcgcctc caagtatgag cagaaacaaa
    13141 gcgaggtggc tgcagcggtt acggttatta cgcgtaatga tattcgagct tatggttgga
    13201 gaacattaag tgagatatta acaagccttc caggtgtttt tgctacttat gattttagct
    13261 acaactatat cggtgtaagg ggttttaatg ttctcggcga ttttaatacg cgtattttag
    13321 tttcaatcaa tggtaaccga atcaatgatg cgacttatga tcaaggtcct atggggcggg
    13381 attttccgct agatgtcgat ttaatcgaac gcatcgagtt catcccaggg ccggggagcg
    13441 cggtttatgg gcagaatgca atgttaggtg tcgttaacat tattactcgc aaaggctcta
    13501 atgtgaatgg cgttgaactt tctgggtctt atcaaactgc agaagtaatg ccccaagaac
    13561 gtgccacctt agggaaaaaa ttcgataacg gtgtcgatgc tttagtttcg gtttcaggcg
    13621 ttcaagcacg cggtgaaaat cttttttttg attttcgtga tgctgctatt tcaggcgttg
    13681 ctcaccatat ggatggcgaa aatattaagc agatttttgc aagagcagaa aaagggcccg
    13741 tttcctttga ttttatctac ggtaatcgca aaaaagacga ccccacggca atttattttt
    13801 cagacccgct catttctggg caatacctta aagatcggcg tctgaattct caattgcaat
    13861 ataacgataa ttttgtcaat gacacgctta atgtacttgg gcgacttttc atcggtaaat
    13921 acgattacga tagcccatta gtttatgagg ggaaaaaaac attatcaacg ggtccaagtt
    13981 tctggcatgg tgctgaatta aggttagttt caagcgcaat cacaaaccat aaattgatgg
    14041 ccggtgttga ataccagaat aatacacata tcaagcagac atttgagtat ttggagagcc
    14101 tcgcggataa tattaccgtt agaagctcag tagttagaac aggggtatat cttcaagatg
    14161 aatggcgcat taccgatacc ctatcatcaa cattgggggt gagatacgat tacaacaact
    14221 ggatcggcaa tcgcctaagt cctcgtggtg ctttaatctg gcaagctaca cctaaagctg
    14281 catttaaagc catttacggt cgtgcacacc gatcccccaa ttcctatgag cgtgattatg
    14341 gagatggcgt ttctcaaatc tcaaacccag ggttaagagg agaaaccatt gatgcagcgg
    14401 agttagtcgc agattacttt cctgaaccca atctaaattt gcgggcttcg ctatatgctt
    14461 gggatgttta caacattatc gcattgggtt ttaatccgtt gttggaagct tcccagtatc
    14521 agcaaaccca cgacaaagta cgcgcaagag gggtagagct ctattttgat aaaacctgga
    14581 attggggagc tcgcctacga agtagcttcg gtattcaaga tgcttcacaa aaaggatcgc
    14641 atattcctaa ttctccccag ctattaggaa agattaacgt tttggttcca attccactta
    14701 tgtctggcct aaaaggcggc tatgagcttc aatatttcgg tgaaagaaaa acgttaaatg
    14761 gtcccaacac cgatagttat tttctctcta acttaaactt aatgacagat attcgttggg
    14821 tgaaaggatt agaggcatcg ctcgctgttt ataatttatt taatgagcac tatttacatc
    14881 cggtttcaga ctcaagctgg cacaatagtt tattgcaacc tggtagaacg gttagattta
    14941 ggctcgagta ccgtttttaa ttaacaatga ttagtcggat tattaacatc gattgcaaaa
    15001 caaatagcaa ttaatgcaat cgatcagcat aaataagcca aaaaaacctt ttaaatgtaa
    15061 acagaactct agctatcatg ggtttatact aatagtcaat ttaataataa cattacccat
    15121 ttggcttacc gctgtgcttt attcacccaa ttcttttgga caacccgaag aaccgactga
    15181 ataccggttg aaagctgcat ttctttataa ttttgcaagc tttcccgagc cacctactaa
    15241 gattgataaa cacagtactt tcaatatgtg tttcctcgat tacatcgctc aaagaaaagt
    15301 aaaagacttg caagttacca ttttattgaa gtctgatccc gataaattaa gtgaatgcca
    15361 tcttttgtac atttcccgct ctgttgctag taataagctt gaaagcattc ttgaccgcat
    15421 aagcagtggc cctattttaa cggttgctga tagtacggga atctgccaaa aagaagttgt
    15481 aactaatatg gggattaatg cgggcaaaac tacgttcaaa acaaatttga cgcctgctca
    15541 cacagcaaaa ttagaactca gctcacaatt acttcgcttt gctaaagagg cttatcaata
    15601 atgaatcccc tattacctgc tacaaccaca ataaaaaata aactacaaaa aattagtttg
    15661 atttcacaaa gtacagcaat gtttttactt gctggattgg ttatattgag tagctttatc
    15721 attaatcttt actcactttt ggagacgagt cagtccaccg cgaaaatcct ggcagaaaat
    15781 gcaactgcaa ctttaatgtt tcaagataaa aattctgcac aaacattact ctactcacta
    15841 aataacctaa gtcgagtcaa tgcggcggct attttcagtg agaatcatca ccaatttgca
    15901 gaatacccaa ataaccatca tgcttttccc cattttgatt ctttaaatga agaaaaaatc
    15961 ttaatcgatg cacaatatat caaattactt caaccaatcc gcttcaatga tcaatcatta
    16021 ggggcgctct atttagaagt cactatgttg cctttatatt ggcaaatgtt atggcaggtt
    16081 ttaatcacaa ttgctgctgc aactattgca ctttatgttg cttatcgact attgcaacat
    16141 ttaaatcgct cagtattgaa tccactcaat cagctctcag aagtcattga acatgttacg
    16201 agcaatgcag actataaggc aagaactccc aaaagtaata tcgtagagct cgatatgcta
    16261 gcgaatgggt tcaataacat gctggaagtc attcaagaac gagattccaa attagccaac
    16321 catcttgatc atttagaaga tgaagtttca aaacgaacat cagagttggt tctcgcaaaa
    16381 gaagcagcgg aagcagcgag taaagccaaa agtgagtttc ttgctacgat gagtcatgaa
    16441 atacgcacac ccatgaatgg catactggga atgacagaat tgttactgaa tactgagcta
    16501 tctaaagatc agtatcgatt tgcggatact gtgcaacgat cgggccagca tttgctcggc
    16561 attattaacg atattctgga tttttcaaaa attgaatccg gtcacatgga gcttgaaacg
    16621 gttgattttg aactggtcaa attaatcgaa gataccctca tcatgtttgc tcaaccagct
    16681 gatgaaaaag ggttagagtt ggctgcacaa ttcatacctc ccaatatacc gattgcgata
    16741 aaaggcgacc catttcgctt aaaacaagtg cttgtgaatc taattaataa tgcgattaaa
    16801 ttcaccagtc aaggcgaagt cattattcgg acacgcataa tagaagaatc tgaaacgcaa
    16861 attcatatta atatttgtgt tgcggataca gggattggta ttcctgaggc ttatcatgac
    16921 aaaatattcc aacatttctc gcaagcggat ggttcaacaa cccgccaata cggtggaaca
    16981 ggccttgggc tcactatttg taagcgttta ttagagctga tgaatggtcg aatttgggtt
    17041 gaaagtagtt cagggcacgg ctccaaattc tggatcgata tcaagatgga gaaatgttca
    17101 cttgcagcca ttgccgcttt accaaatcta gaaatactgc acaataccag agtattaatc
    17161 gttgatgaca atcaaacaaa tcgtgaaata ctagaattac aattaaaaag ttggaatgta
    17221 aaagtaaatt gtgctgagaa tgcggagcga gcactaatca tgatgagcaa agcagtagaa
    17281 gattgtgatc catatcggct agctatcctc gatatgcata tgcctaaaat ggacggcctt
    17341 cagcttgcaa aaattattgc atccgatgct aacctgagca aaactcgcct catgatgctt
    17401 acctctacct atagcaatgc ctcacaatta gagcgacaaa atgttggcat tctacgctgc
    17461 gctaataagc cagtacgaca acgggaacta ctcgaaatca tcctagatgt cttgcaacgc
    17521 tctgataaat cagcctattc agaaacacca ataaaacaat ctacttcagc attaccagca
    17581 aaaaacctga cacaaggcaa ggtgctactg gctgaagata acttagtcaa tcaagaagta
    17641 gcgaaagcga tgcttgctaa gttaggtttc aaggtcgata ttgctaacga tggtcagcaa
    17701 gcgttagatt ttatgaataa acaacgttac gatatcgttc tcatggattg ccaaatgcca
    17761 atcatggatg gatttgaagc gacgacccag attcgtaaac aatacggtca aacaataccg
    17821 attattgcgt tgactgctaa cgcgactgaa gatgatcgta accattgcat cgaagcaggt
    17881 atggatgatt ttctttcaaa accctataca ttgaatcagt taaatcaagt cctcactaaa
    17941 tgggaaacaa aaactcaatc aaacttagtc gatgcaatgt cagaatctca tgtaccaaaa
    18001 gaaaatactg aaataaaaaa aatgccttta ttgaatcccg tattgttaga tcagatccgt
    18061 agccttgata catcaaatgg acacgaatta gtcaataaga ttttatgcgc ctttctcgag
    18121 tctgcagaaa aatatatcga gttacttgac cgagcattta atgataaaga tattgaaacc
    18181 attaggaaaa cagcacacac attaaaatca agttcagcta acattggtgc tgaggaatta
    18241 tcaacaatat tcaaacaaat tgaagcatat agtaaaaatg gggaattagc gctcgctgaa
    18301 tcgttgtatt cagggatgtt agctatttat cagcaagtaa taatcgaagt taaagcgatc
    18361 atcaatcaat ctcaatgagc agaactttta aaatactact tgctgacgat gatgtaacga
    18421 ttcgcttttt gatgcaagcc gctttggaac aagcaggctt tgaagttatt ttggcaagta
    18481 atggggaaga agcgattcag ttatttaata catcctcacc cgatatggtg atgttagatg
    18541 ttgagatgcc ccaaatgaat ggttaccaag tgtgtactta cctacgtaag caaattggca
    18601 atgaacttcc gattatcatg gtcaccggta tggatgatgt tcaatcaatc gatcatgcat
    18661 ttgaagtggg cgccactgat tttattgcaa aaccaattaa ctggaatctg atcaattatc
    18721 gattgttata tctcaggcgg gcctacctca acttgttgga tttgaagaat gcaaatgccc
    18781 gtagcaaagc tatttttagc gccattcccg acacgatgtt catcatagat aataatggcc
    18841 atatcgttga tacacaaagt agttctgata aaaatacctt tttaaatttc caaacgggag
    18901 atgatttaaa ccagcgaatg cctgcaaata ttacccaggc ccatttggaa gcagcaggaa
    18961 gagcccgtaa atatggcgca gtcgaacact ttgagtatca attaaatggc gatagtgatc
    19021 gtgaaaaata ctacgaaagt cgtgttgtag ccattgattc caatgagatc ttatgcttgg
    19081 tacgggatat tacggagcga aaagattctg agaatcgaat ctttcggtta gcttattttg
    19141 atagcttaac cggacttcct aatcgtcaat cgtttattga taggctaaaa cgcgaaataa
    19201 aacgtgcaaa atatatcggc tcgaaacttg ctatcttatt tcttgatctc gatggcttca
    19261 aaagtattaa tgacacaatg ggccataaca ccggggacgt tattcttcag tgggctgcag
    19321 atcgacttca aagtagcact cgctcctctg attttgtatc tcgtagcaac acaactggct
    19381 ccgaaataga gctcgcacga ttggggggag atgaattcac tgtcgtaatc ccgaacctga
    19441 cacgggcaga agatgcattg gtacttgcgc atcgaatacg tgagaccatg cgacgtccat
    19501 tccgattaga cagccgcgat gtagtactca ccacaagtat tgggattgca ctgtatcccg
    19561 atgatggtga agatgttgaa gcattactta aacacgctga tacagcaatg tatcatgcaa
    19621 aaacggaagg gcgagataat tgccaatttt acagtgttac gctgacacaa gaagcggaaa
    19681 aacgcctgaa tttagaaaat gatttgcgta atgcattaaa aaataatgaa ttccatttgc
    19741 tatatcaacc tcaatttgat gttgagagat cacactttaa gtccgctgaa gcgttgattc
    19801 gctggaatca tcccaagcaa ggattagtct ctcccttaga tttcatccca ctcgccgagg
    19861 aaaatggctt gatcataccg atcggagagt gggtattaag aactgcctgt agcgatgcgg
    19921 cccattggct aaaaaatggg ggtgatttaa aagttgctgt caacttatct ccgttacaat
    19981 ttaaaaaccc tgatttaatc aaatcagtgc tttcgatttt aactgagaca gggttgccgc
    20041 ctcataaatt gacccttgaa gttactgaag gcgcatt
//
LOCUS       DAOQKQ010000027        19760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_1211_length_19760_cov_6.177823, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000027 DAOQKQ010000000
VERSION     DAOQKQ010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 19760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19760
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1211_length_19760_cov_6.177823"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="PKZ49_03000"
     CDS             complement(<1..486)
                     /locus_tag="PKZ49_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35558.1"
                     /translation="MPQTNQAQIAYILKGFPRLTETFISNEIYLLEQLGTKLVLFSIK
                     PGELNKQHEVIQKIKSPLTYLPSVTSLSGSYLLIWVLRNIRPYLKHHANMVWVSPGRY
                     FATFRKACALAWQHRNGFLRLRKVFIKEFIQAGFIANQIQSRPNILLLHAHFCHGATT
                     VA"
     gene            complement(473..1804)
                     /locus_tag="PKZ49_03005"
     CDS             complement(473..1804)
                     /locus_tag="PKZ49_03005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HND35559.1"
                     /translation="MSDRSNQVDVSHPNRVTGDSVQVSTRVGILVRCYPKLSETFILG
                     EILGLEKENIPLHIFSLFPPSDQLVNPAVNFVKASVTTLANGDSAMSMHIIKKHLQFF
                     VSHPKAYLSMLARILFSNEYGKIKDFWFAGKLATELISQKITHLHVHFISTPAAIGRI
                     ASDLCNITYSISAHAKDIYLSRADELAKKLHTAAFTVTCTQYNWVYLRSKTNYPEKIH
                     CMYHGINLAPFNQVLAASNDQPTDLPLILSVGRLREKKGFSTLIAACKKLSDLGQPFE
                     CIIAGYGPEQDRLQLQIDTLSLQNQVKLVGKKSHPEIIQLYSRARVFTLPCRVASDGD
                     RDGIPNVMLEAMAMGLPVVSTIVSGIPEVITHGENGLLLEPDDVTGLAAALLKLIRDP
                     LTGKAFGMKGRALIEEKFSTSRNLEILIGLLKQADQTLQKSSNKVNANATN"
     gene            complement(1801..3144)
                     /locus_tag="PKZ49_03010"
     CDS             complement(1801..3144)
                     /locus_tag="PKZ49_03010"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HND35560.1"
                     /translation="MKRIMIYSHDTFGLGNIRRMLSIAEFIAAGREDYSILLITGSPM
                     LHAFRISQKIDYIKLPSLQRDIEGNYAVRSLALSMDEIIAIRANIILTTVEDYQPDIL
                     IVDKKPLGICAELQPAMEYLNRGENARHVRRLLLLRDILDSSEVTREIWKKRGYYDAI
                     EQYYDRVLVVGQSEIFDLTKEYAFPPAAQKKLEYCGYIRREAGLKGRQVVRSELGLSD
                     QHKLVFVTPGGGKDGFRLITNYLMGIDRYKNDSVYSLVLCGPEMLEEHVVTIKALAHG
                     KSRCLIQSFTDDLMSYMAAADVVVSMFGYNTACEILTLGKPAVVVPRIHPVREQWIRA
                     ERLAELGLVKIIHPELLESESLYQAIDSLLFQGERLRIPEQQINLNALPVIKQIIADE
                     IDIQNQLVNLSTGVTKTLSCLDRQPMEFDQQLAGQSKSLTPDLSLQSSQIEEKEL"
     gene            3448..4338
                     /locus_tag="PKZ49_03015"
     CDS             3448..4338
                     /locus_tag="PKZ49_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6502 family protein"
                     /protein_id="HND35561.1"
                     /translation="MSTIPPTLVSALKQVLRPLIKLMLAKGLTFTYLIELLKEIFVEI
                     ADKDYKINNKPSTDSHLSLLTGIHRKDIKRLRNYSQTVEEAIPPNVSLGSRLVSIWTS
                     ETRFLDENNQPKPLPRFIKEGGEISFEGLVASVSKDIRSRVVLDEWLRIGIVHFNDEK
                     CVCLNTAAFVPSQGFDEKAYYFGESLHDHAAAATSNLLGGAKPYLERSVYYDALSTSS
                     IQQLAEQSEKLGMETLLAINKQAMALEQEDILGKNPKYRMRFGIYFYSEPVTPPISNN
                     ELDQPLPRSLVEAENNITQS"
     gene            4335..6131
                     /locus_tag="PKZ49_03020"
     CDS             4335..6131
                     /locus_tag="PKZ49_03020"
                     /inference="COORDINATES: protein motif:HMM:NF039822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HND35562.1"
                     /translation="MKPWLHFLLHTVTLVITILLAVPNVFAKNNCETSLTQVNPAAGI
                     GGTGSKVAESGGGGTGIYAGGGIGGTGHPEKGIGGTGSKLAEDGGIGGTGIIGTITGF
                     ASVCVNGVEVHYDNNTPVTVDGRLSNARELAVGQVVITRAIGSGQEVTAKHIAVVHAA
                     VGPISHINPLTREMHVLGQVVQVTESRDLSNFKPGDWAQISGHRLANGAIIASRIEAA
                     TTATDAKINGHITHVDAKGFEVNGARIQHDAKSFPTKVSVGMEIAVAGHWNGNHLQAQ
                     HMQIEPTRQAIGQVSHLVIEGYVHAVRGKEINIANQPITLDSNAKVTGDNKEDLKLNQ
                     RVQVSGRVGPDQRIIADRVELKPESPTSLPERIDRNQTENDNRNKKSDSTNELGPRPN
                     NKSDEDKSNQNRSGESDRGKENSKRESSDHSGKQEAASDNKSRSSESLSSGKDGRDQT
                     DRQSQSTRSEDSERDQKNLSSDHDSRKSDSTSSSDTIAKDKLEKPIEEKPSNSSEKTD
                     SKLRLDHPSDLSKQDREQSESKTIDADSQMVPERMPMEKSQISDQPEKSTISDRDSLD
                     RPERPRDSDDLTERPSTDLNNIRDVMPDRIRD"
     gene            6151..7530
                     /locus_tag="PKZ49_03025"
     CDS             6151..7530
                     /locus_tag="PKZ49_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35563.1"
                     /translation="MSQTITRFSNRSIWTQTAVALCFLWNCSPISANGLSLSGGMDFT
                     TGNYGGSSATDIWYAPFTARYDYKKFSFRTTIPYLNLTGPGNVLGPGIGGVTIGDGGI
                     VPGVGGGSIGGGSGGVFVCNEEDGDCSTTEDDPDDDNSGSGGGGSDPDDDDNSGSGGG
                     GSDPDDDDNSGSGGGGSDPDDDDNSGSGGGGSDPDDDNSGSGGGGSDPDDDNSGSGGG
                     GSGPDDNSGSGGGGSGSDDNSGSGGGGSDPDDDNSGSGGGGSGSDDSSGSSSGSSEAD
                     ESPFVESALNQVGIGFPSNAPRSNHSGLGDVVTALTYNVFNHEPTGIIFDITGRIKAP
                     TASAANNLGTGQVDYAIQGDLIKSMSRFMLSASFGYRILGNPSGVNFHNVFYGAAGIA
                     FRYSPDTTIGTSFNIGQSPVRLQDSRDFTLYVSHRLNDNFRLNIYGLRGASERSPDWG
                     GGINLRYVF"
     gene            complement(7621..8073)
                     /locus_tag="PKZ49_03030"
     CDS             complement(7621..8073)
                     /locus_tag="PKZ49_03030"
                     /inference="COORDINATES: protein motif:HMM:NF012342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HND35564.1"
                     /translation="MQQMQKGFTLIELMIVVAIIGILAAVAVPAYQNYTIKSRFTEVI
                     NATAPFKTAVELCVQSGNCLNGANIVVAATGARNQIEVPDDQTAAVGKIGAITVSNVG
                     VILATPVASQGVTAADTYQLTPARQANGTILWTIGGGCLNSTNAGKIC"
     gene            complement(8277..11069)
                     /gene="glnE"
                     /locus_tag="PKZ49_03035"
     CDS             complement(8277..11069)
                     /gene="glnE"
                     /locus_tag="PKZ49_03035"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HND35565.1"
                     /translation="MILHGSHTTTESLLEKILPFSRYVQRLLASEPDLQGYLMDKGAS
                     QFLREEMLDFLNSACVAPSLNEAGLNAVLRKLRKRVMLRLAVRDLAGLAGLPEVMMTM
                     TQLAEVTIQFALKHHQIWMSHPEHYGEPIGEKSASPRQLLVIAMGKLGGGELNVSSDV
                     DLIFLYPEEGETNGKKSISNHEFFTRLGRKLIASLNDYTVDGYVFRVDMRLRPHGENS
                     PLTISFAMLEEYLTTQGREWERHAWLKSRVIVGSLAEEAILMEQIVRPFVFRKYLDFE
                     AYEAMRRLHAQLRKEVDRREMHDNIKLGPGGIREIEFITQVFQLIRGGRDADLCIRPT
                     LDTLGRLLEKQPLPSQTIQELIDAYQFLRKLEHRLQYLDDQQTQSIPENADDQALIAT
                     AMGFASPPELFAKLDIYRSQVTRHFELIFAVPRKSPTHDILTALWQEQPSDLMQVKAA
                     THQLTTLGFSDPEKISARIQQFYVSQFFQKMPLASQQKITAVMPTLVEAVAQFPPIEL
                     TLERMLQLLEHISQHPAYLALLLEHPQTLPRVAKLVNSSQWASNYLGRHPILLDELLA
                     PQGLYQLPDWKALKVELMRQLNHINSPKKNITEWQMDVLRHFQHAQVFQLLAVDLEGV
                     LLLETLSDHLTELADLILGNVLNLAWQGLKKKHQEQPVFAIIGYGKLGGKELGYSSDL
                     DIVFLYDDNHPDALEIYTKLSQSINTWLTSHTSAGGLYETDLRLRPEGTSGLLACSLD
                     AFKHYQQSQAWVWEHQALTRARFVVGNEQMGAAFEAIRKEILCKPRDLTKLKQDILMM
                     REKMLDAHPNPTSLFDIKHDRGGIIDVEFMVQYLVLGYSNQFPQLTGNIGNIALLKLA
                     GNLGLIPSEMAQHVLSTYREYRRIHHRLRLSGDPELTGAASTKEASISYARIEATQLN
                     DARRVVLQLWEEVFAK"
     gene            11250..15134
                     /locus_tag="PKZ49_03040"
     CDS             11250..15134
                     /locus_tag="PKZ49_03040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HND35566.1"
                     /translation="MNQQTPRHQPYSHSIARLSIVCLFALAALFSVTILALRYWILPN
                     IDNYRLEIAQIISKRAEQLVSIDRINAHWNGVHPYLQLHGVKVYDQAGTPALILTEVE
                     GTLSWHSILHQELRFREIKIDRPTLIMRRDAHNVIHIAGTNIYDEKNDTENSFIDWLL
                     RQEKLVINQTDIYWLDEYRNAPALYLKIVNLLLHSHNLTNRHQLGLRVAALDSTAMSM
                     DIRGDFTGKSIESFLHWQGRLYFELKQIDLAVWRQWIPFPDEIDIRQGKGALRGWLTL
                     AEGTITDWLIDTALHQISANLGTNLPKLELDFLQGRIDVKQSNQSGSLVQEWTAHQLN
                     FKLAHQQIITNPINAIWRQYHDPKQSESSNYLQIDEIDVGVWANLLEYLPVDASVRKQ
                     VEKYSPHGKIEHMQLSWKGSWSEPHAYQLDTQFNDLSLNNIQIGHLISIHGLSGNIQG
                     TEQEGRLNLNSQSVSLQLRQPFPQSIVFDTLATRVGWKKIAEQESTTYKIEDLAFNNQ
                     HLAGSAIRGSYVHKQNKPDWIDIQGNLNQADVNGLSRLLLPFTNKTQLAWIDESSLSG
                     QLKNTQFNIQGNLSDSSANDFSWSFATEMVNISASIGAQWPSITNISGHLTASSTLIN
                     AIVSEGNIETIQFKDTSIHIADLQSKKPLLKIKGEATGKTKQIIQLIKESPLTQNTFD
                     IDIPGVITGKGKLQFESTIPILSKEKIVDIDLKGQYQFINNQIDLGPELPAISKVNGK
                     VSFTHNTWDFDEIHAQLLGGPLVIRSKPLNNDKKQIALEGQANFDRFNTRLSQKSPSA
                     LQFWLQHIHGTTDWTAIIDMNNSQITALQIASSLNGVTSVLPAPFSKTHDEIIPLTFT
                     KTAIDSANEKLFCQLGEKVTTEIHRTQTKQGDFIPVRGVMSFGNRSATLPSEKLTLMH
                     GPIPLLEWDKWQEMLDQHEAIVGLSKQQSRGIDALLTHHIRFDLNVGVLDFLGSRFND
                     FILHADKQGSEWTTDVTSREVTGKIYWNATGKKKATARLKKLVVPESLPENGSAERKK
                     HESQNWPIIDLHADEFFYKEKLLGQLTLLAEQNKENWVVDQMEISHKHSIFTAKGIWK
                     NRQEPFSMYAEIKLTTSDIGKFLNRVGYPDRIARGEGSLEGHLSWQGKPFSVDFPTLS
                     GQLKLAAKHGQFTKFKPGMSKLLGIFDLKALPRRLTLDFYDIFSQGFGFDDILGNVRI
                     DHGIATFVDLEIAGSSAYLAVSGEINLDQETQSLRIKMFPSLGLVTPVIGIASMIADL
                     SLKDPFGRVMFNEYAITGTWDEPLVKKLSEEN"
     gene            15185..16048
                     /locus_tag="PKZ49_03045"
     CDS             15185..16048
                     /locus_tag="PKZ49_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HND35567.1"
                     /translation="MLNSTNSFSSSNSDKEENSKIRVAAIQMASGPSVSANLEEALRL
                     IELAVTQQAKLVVLPEYFCIMGMQDTDKLKVREKPGEGVIQTFLSETAKRFRIWLIGG
                     SVPLISQIPDKIFNSCLVYNDAGEQVARYDKIHLFGFSHGNERFAEEKTIKAGDKVIA
                     IDSPFGRIGLSICYDLRFPELYRMMGKIDIILAPSAFTAITGKAHWETLIRARAIENQ
                     AYVIAPAQGGYHVNGRETNGDSMIVDPWGGIMNRLSRGPGVVVAAIDPAYQASVRTSL
                     PALEHRCLYPQ"
     gene            16106..17584
                     /gene="tldD"
                     /locus_tag="PKZ49_03050"
     CDS             16106..17584
                     /gene="tldD"
                     /locus_tag="PKZ49_03050"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HND35568.1"
                     /translation="MVNHKTDMQIDREEHFSIAERCLLTPFQLDTHRLDQVFAAMLSH
                     HIDYADLYFQHNRTEGWVLEEGIVKSGSFNIDQGVGVRALSGEKTAFAYSDDISISAL
                     HSAAKATRAIANQGNSSSARIITHNNDHHPLLYLPHDPITSITDSNKVQLLEKLERYA
                     RTLDNRIVQVLASLSGEYEVIMVIRSDGKLVADVRPLVRVSLQVIAEENGRREQGVAG
                     GGGRFDYSYFTDEILSNYAQQAVHQAIINLSAKPAPAGTMTVVLGSGWPGILLHEAIG
                     HGLEGDFNRKGSSAFSDRIGERVAADGVTVVDDGTLVNRRGSLTVDDEGNPAQNTVLI
                     ENGVLRGYLQDSLNARLMKQPLTGNGRRESFAHIPMPRMTNTYMLNGDKAPEEIIASV
                     KQGLYAVNFGGGQVDITSGKFVFSAAEAYMIENGKITYPVKGATLIGNGPDVLTRVSM
                     IGNDLSLDPGIGTCGKEGQSVPVGVGQPTMRIDGLTVGGTGN"
     gene            17674..19377
                     /locus_tag="PKZ49_03055"
     CDS             17674..19377
                     /locus_tag="PKZ49_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 catalytic subunit"
                     /protein_id="HND35569.1"
                     /translation="MTEELTGAEITIRCLQEEGVEYIFGYPGGAVLFIYDELFKQDKV
                     KHILVRHEQAALHAADGYARSSHKVGVALVTSGPGVTNAVTGIATAYMDSIPLVIISG
                     QVPTAAIGQDAFQEVDTVGITRPCVKHNFLVKDITELAATIKKAFFIASSGRPGPVLV
                     DIPKDVTQQKTHFSYPNSIHMRSYNPVTNGNTKQIKKAAELILSAKRPMFYSGGGVIL
                     DNAAGVLTELVQLLDYPCTNTLMGLGGFPATDKHSVGMLGMHGTYEANMSMQYCDVLI
                     AVGARFDDRVIGNPKHFYSEDRKIIHIDIDPSSISKRVKVDIPIVGSVSQVLKELIKL
                     IKASKEKTDAIALKEWWKQINLWRERDCLKYDRSSHVIKPQMVIEKLYAITKGDAFIT
                     SDVGQHQMWAAQFYPFDKPRRWINSGGLGTMGFGLPSAMGVQFANPTGTVACITGEAS
                     IQMCIQELSTCKQYQLPLKIINLNNRYMGMVRQWQEFFHGNRYSESYMDALPDFVKLA
                     ESYGHVGMKIEKPGDIEGALKDAFKLKKRLVFLDFITDQTENVFPMVPGGKGLSEMIL
                     V"
     gene            19381..>19760
                     /gene="ilvN"
                     /locus_tag="PKZ49_03060"
     CDS             19381..>19760
                     /gene="ilvN"
                     /locus_tag="PKZ49_03060"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HND35570.1"
                     /translation="MRHIISLLMENEAGTLSRVAGLFSARGYNIESLNVAPTEDPTLS
                     RMTLVTIGSDEVIEQITKQLNKLIEVVKIIDLTEGDHIERELMLVKVKAIGDGRDEIK
                     RLTDIFRGSIIDVTDKSYTIELTGT"
BASE COUNT         5936 a         4298 c         4193 g         5333 t
ORIGIN      
        1 agccactgtc gttgcgccat gacagaaatg cgcatgcaac aacaaaatat taggacgact
       61 ttgaatttga ttggcgataa aaccagcttg gataaattct ttaataaata ctttgcgtaa
      121 gcgtaagaaa ccattccggt gctgccatgc taaagcacaa gctttccgaa aagtagcaaa
      181 gtaacggcct ggactcaccc ataccatgtt tgcatggtgc tttaaataag gtcttatatt
      241 tctaagtacc cagatcaaca aataactacc ggataatgat gtgactgaag gcaaataagt
      301 caaaggagac ttaatcttct ggatgacctc atgttgtttg tttaactccc ccggtttgat
      361 agaaaacaag acaagttttg tgcctaattg ctctagcaag tatatttcgt tcgaaataaa
      421 ggtttcagta agcctaggga accccttcaa tatataggct atttgggctt gattagtttg
      481 tggcattggc attcacctta ttactgcttt tctgtagcgt ttgatcagcc tgcttcaaca
      541 aaccaatcag tatctccaga tttctacttg tagagaactt ttcctcgatc agcgctcttc
      601 ctttcattcc aaacgccttg ccagtgagtg gatctcgtat cagcttcaat agtgcggcag
      661 ctagcccagt aacatcatcc ggttcaagca ataagccatt ttctccgtgc gtaatcactt
      721 ctgggatacc ggaaactatc gttgaaacca cgggtaaacc cattgccatt gcttctaaca
      781 taacattggg aattccatca cgatcgccat ctgatgccac acggcatggt aatgtaaata
      841 cccttgcccg actgtataac tgaataatct ctggatgtga ttttttcccg actaatttca
      901 cctggttttg gagtgaaagc gtatcgattt gtagctgcaa cctatcttgt tcagggccat
      961 aaccagcaat aatgcattca aaaggctgac ccaagtcgct taattttttg caagcagcga
     1021 ttaatgtgga aaatcctttt ttctcacgta aacgtcctac actcaatata agcggtaaat
     1081 ctgtaggctg atcattgctc gctgcaagta cctgattaaa aggcgctagg tttatgccat
     1141 ggtacatgca atgaattttt tctggataat tcgtttttga tctaagatag acccagttat
     1201 attgggtaca tgtgaccgta aaggcggcag tatgcaattt ctttgctaac tcatcagcac
     1261 gagacaaata aatatctttg gcgtgcgccg aaatactata agtaatgttg caaaggtcgc
     1321 ttgcgattcg cccaatcgcg gcgggtgtag aaataaaatg tacgtgaaga tgtgtaattt
     1381 tttgactgat cagctcagta gccagtttcc ctgcaaacca aaaatctttg atcttcccat
     1441 attcattgct gaataaaata cgtgccagca ttgatagata agctttagga tgagatacaa
     1501 aaaattgcaa atgttttttg atgatatgca ttgacattgc actatcacca tttgccagtg
     1561 tcgttaccga tgctttgaca aaattgacag caggattaac tagctgatcg cttggcggaa
     1621 ataacgaaaa aatatggagt ggaatgtttt ctttttctag tcctaaaatt tctcccaaaa
     1681 taaatgtttc agaaagttta gggtaacacc taaccaatat gccaacacgt gtactcactt
     1741 gcactgaatc gccagttaca cggttaggat gactgacgtc gacctgattg gaacggtcac
     1801 tcataattct ttctcttcaa tttgcgatga ttgcagagat aaatcggggg tcaaggactt
     1861 ggattgtccc gctagctgct gatcaaattc cattggttgg cgatctaaac acgagagtgt
     1921 cttagttacc cctgtagaga ggttgacaag ctgattttga atatcaattt catcagcgat
     1981 gatctgcttg attacaggaa gcgcattcaa gtttatctgc tgctctggta tccgtaatcg
     2041 ttcaccctga aataacaatg aatcgatggc ttgataaaga ctctcacttt ctaaaagctc
     2101 cgggtgaatg attttaacca aacctaattc tgctaggcgc tcagcccgta tccactgttc
     2161 tctaacaggg tggattctag gcacgacgac agcaggtttt cctaacgtca aaatttcgca
     2221 cgccgtgtta tagccaaaca tcgatactac gacatcagct gccgccatat agctcattaa
     2281 gtcatcagtg aatgactgaa tcaaacatcg ggattttccg tgagcgagcg cttttatggt
     2341 tacaacatgc tcttcaagca tctctggacc acataacacc aatgaatata ccgaatcgtt
     2401 tttataacga tcgatcccca tcagatagtt ggtaattagt ctaaatccat cctttccacc
     2461 acctggcgtc acaaatacca acttatgctg atcgctgagg ccgagttcac tccgtactac
     2521 ttgccggcct ttgagtcccg cttcacgacg gatgtagcca caatactcaa gtttcttttg
     2581 tgctgctgga ggaaaggcat actctttggt caaatcgaag atttctgact ggccaacgac
     2641 cagaactcga tcataatatt gctcaatcgc atcgtaatat cctcttttct tccaaatctc
     2701 acgtgtgact tcagaactat caagaatatc gcgtagcaat aacaatcttc gaacatgccg
     2761 agcattctca cctcgattta agtattccat ggcaggctgt agttctgcgc aaattccgag
     2821 tggtttctta tcgacaatca aaatatctgg ctgataatcc tcgactgtgg tcaggataat
     2881 gttggcgcgt atggcaataa tctcatccat actcagtgcc aatgagcgga cagcatagtt
     2941 gccttcgata tcacgttgca gagaaggcaa tttaatgtaa tcgattttct gggaaatccg
     3001 gaaagcatgt agcatgggtg atccggttat gagcaaaata ctgtaatctt cgcgcccagc
     3061 cgcaatgaac tcagcaatcg ataacatgcg ccggatattg cccaacccga aagtgtcatg
     3121 cgaataaatc atgatgcgtt tcattattta tatccctact tttcagtttg ataacgttca
     3181 gtaccttaaa tttggtttca ggattccaag tcgcctttac ttaacgaaac agaattatta
     3241 tcgtaaatcc aattatatgt gtgaatattt ccctcgattt gatataatat acgcatggtc
     3301 gattcctgtg tcaacacatt gatgtaaata tttcccacac atatgataga attgctgcta
     3361 taactgattg gcaaggatcg aatatttatc tagcaacgaa ccgagataca aacttttccg
     3421 ataatcagaa ttacaataaa tataacaatg tcgactattc cacccacact tgtatccgca
     3481 ttaaaacaag tgctgcgtcc gctgattaag cttatgctag ccaaagggtt aacattcact
     3541 tacctaattg aattactaaa agaaattttt gtagaaatcg ctgacaagga ttataaaatc
     3601 aacaataagc cctcgacaga cagtcactta agtttactca cagggattca tcgtaaagac
     3661 atcaagcgac ttcgaaatta ctcccaaaca gtagaagaag ccattccgcc caatgtttcc
     3721 ttaggatctc gcttagtgag catttggacc agtgaaactc gtttcctaga tgaaaacaac
     3781 caacccaagc ctttaccccg atttatcaaa gaaggaggag aaatttcctt tgaagggcta
     3841 gttgcaagcg tcagtaaaga tattcgttca cgcgtagtgc tggatgaatg gctacgcatc
     3901 ggcatcgttc atttcaatga tgaaaaatgc gtttgcctga atactgctgc ttttgtaccg
     3961 agtcagggat ttgatgaaaa agcctattac ttcggagaaa gtctccatga ccacgcagca
     4021 gcagctacta gcaatctact cggcggtgcc aaaccttact tagaacgcag cgtttattat
     4081 gacgcactga gtacatcttc tattcaacaa ttagcggagc aatcagaaaa actcggaatg
     4141 gaaacgctgt tagcaatcaa taagcaagca atggccttgg agcaggaaga tattcttgga
     4201 aagaacccta agtatcggat gcgttttggg atttatttct atagcgaacc ggttacaccg
     4261 cccatatcca ataatgaatt ggatcaacca cttccgcgca gcttagtgga agctgaaaat
     4321 aacataacgc aatcatgaaa ccctggcttc acttcctctt acatacagtc acgctcgtaa
     4381 tcacgattct attggctgtt ccgaatgtat ttgccaaaaa caattgtgaa acgagtctaa
     4441 ctcaggttaa tcccgctgct ggcattggtg gaactggttc aaaagtagct gaatcgggcg
     4501 gtggtggcac tggaatctat gcaggaggag gaatcggtgg tacaggtcat cctgagaaag
     4561 gaatcggtgg cacgggtagc aaacttgctg aagatggcgg tatcggtggc accggcatca
     4621 tcggaaccat cactggtttt gccagcgtct gcgtcaatgg tgttgaagtt cattacgata
     4681 ataatacacc tgtaactgtg gacggtcggc tttccaatgc ccgtgagctt gccgttggac
     4741 aggtggtcat cacccgtgca ataggttctg gacaagaagt cactgccaaa catattgcgg
     4801 tcgttcatgc ggcagttggc cccatcagcc atatcaatcc actgactaga gaaatgcatg
     4861 tgttgggaca agtcgtacaa gttactgaat cccgtgatct ttctaacttt aaaccaggcg
     4921 attgggctca aataagtgga caccgtttag ccaacggcgc aattattgca tcacggattg
     4981 aagcagcaac taccgcaacg gatgccaaaa tcaatggcca tatcacacat gtcgatgcca
     5041 aagggtttga ggtcaatggc gctcgaatac aacatgatgc gaagtctttc ccaacaaaag
     5101 tttcagtagg aatggaaatt gcggtagccg gacattggaa tggcaatcat ctgcaagcgc
     5161 agcatatgca aattgaacca acacgacaag ccattggcca ggtaagtcat ctcgtaatcg
     5221 agggctacgt ccatgccgta cgtggtaaag aaataaacat tgccaatcag ccgatcacac
     5281 ttgactccaa tgcgaaggta acaggtgata acaaagaaga tcttaagtta aatcaacgtg
     5341 tccaggtcag tggtcgagta ggtcccgatc agagaattat tgctgatcgg gtagaattaa
     5401 aaccggaatc accaacatcg ctaccagaaa gaatcgatcg gaatcaaacg gaaaacgata
     5461 atcggaacaa aaaatcggat tcgaccaatg aactggggcc tcgcccaaac aataaatccg
     5521 atgaagataa atctaaccag aatcgatctg gtgaatctga ccgcggaaaa gagaacagca
     5581 aacgtgaatc atccgatcat tcaggaaaac aggaggctgc ttctgataat aaatcccgtt
     5641 ctagcgaatc tctcagctct ggaaaagatg ggcgagatca gactgaccgt caatcacaat
     5701 caactcgatc tgaagattcc gaacgtgatc agaaaaactt gtcttcagat catgattcac
     5761 gaaaatcgga ttcaacgagt tcatcagata ctattgctaa agacaaattg gaaaagccca
     5821 ttgaagaaaa accatctaac tcgtcagaaa aaacagatag caagctaaga ttagatcatc
     5881 cctctgattt atcgaaacaa gaccgcgaac aatctgaatc aaaaaccata gacgctgatt
     5941 cacaaatggt tcctgagcga atgcctatgg agaaatcaca aatttcggat caaccagaaa
     6001 aatcaacgat ctcagatcga gattctctgg atcggcccga gcgccctagg gattctgatg
     6061 atctaactga acgcccaagt accgatttaa acaatatcag agatgtgatg cctgatagaa
     6121 ttcgagatta acccctgcta atttataact atgagtcaaa ccattacccg attttctaat
     6181 cgatcgattt ggacacaaac agccgttgca ctctgttttc tgtggaattg ctcgccaatc
     6241 tcggcaaatg gactcagctt gagtggcggt atggatttta ccaccggtaa ctacggcgga
     6301 tcatcggcaa cggacatctg gtatgcgcca tttacagcac gctacgatta taaaaaattc
     6361 tcattcagaa caaccatccc ttatctcaat ttaacgggcc caggcaatgt actcgggcct
     6421 ggtattggtg gagttacgat tggtgatggt gggatcgtcc ctggagtcgg tggtggaagc
     6481 atcggtggcg gaagtggggg ggtttttgtt tgtaatgaag aagatggtga ttgctcaacg
     6541 actgaagacg atcccgatga cgataattcc ggttccggcg gcggtggttc cgatcccgat
     6601 gatgacgata attccggttc tggcggtggt ggttccgatc ccgatgacga cgataattcc
     6661 ggttccggtg gcggtggttc cgatcccgat gatgacgaca attccggttc tggcggcggt
     6721 ggttccgatc ccgatgatga taattccggc tctggtggcg gtggttccga tcccgatgat
     6781 gacaattccg gctctggtgg cggcggatca ggcccagatg ataattccgg ttctggtggc
     6841 ggtggatcag gttcagatga caattccggt tccggtggtg gtggttccga tcccgatgac
     6901 gataattccg gttccggtgg cggtggatca ggttcagatg atagctcagg ctctagtagt
     6961 ggcagctcag aagcagatga gtctccattt gttgaaagcg cactcaatca agttggaatc
     7021 ggttttccat cgaatgcacc tcgttcaaat cattcagggt taggtgatgt tgtcactgca
     7081 ctcacttaca atgtcttcaa tcatgaaccg acaggaatca tttttgatat cacaggccgc
     7141 ataaaagctc caactgccag cgcagccaat aatttaggga ctggacaggt cgattatgcg
     7201 atacaaggtg atttaatcaa atcgatgtca cgatttatgc tcagcgcgag ctttggctat
     7261 cgaattcttg gaaacccgtc aggggtaaat ttccataatg tgttctatgg cgctgcgggt
     7321 attgcattcc ggtattcacc cgataccacc atcggcacta gtttcaatat tgggcaatcg
     7381 cccgttcgac tgcaagattc aagagacttc acgctttacg tgtctcatcg ccttaatgat
     7441 aactttagac tcaacattta cggactcaga ggcgcgtcag agcgcagccc tgactggggc
     7501 ggtggtatta acttacgcta cgtgttttaa atgattgcca actaaaaatt tgcctaaaaa
     7561 gtaaaagaca aaaaaatacc ccattgctgg ggtattttgt aaaaagccgt tatgaagttc
     7621 ttaacaaatc ttccctgcat ttgttgaatt cagacacccg ccaccaatcg tccataatat
     7681 agtcccatta gcttgacgag ctggggttaa ttgatatgta tcagctgcag taacaccctg
     7741 gcttgcaacg ggtgtagcta gaataacgcc aacatttgaa acagtaatag ccccaatttt
     7801 accaacagct gcagtttgat catctggtac ttcaatttga ttacgtgcgc ctgtagcagc
     7861 aacaacaatg tttgcaccgt ttaaacaatt acctgattgc acacatagct ctacagccgt
     7921 cttaaatggt gctgttgcgt taatcacttc tgtaaatcta gatttgattg tatagttttg
     7981 atacgcaggt acagcaaccg ctgccaaaat accaataatg gcaacgacaa tcattaactc
     8041 gatcaatgta aaaccttttt gcatttgttg catgtatcat ctcctttggg ttgaaaaaaa
     8101 acacactcgc gtgtggtata agccgtgtag tgaaggcaaa cccctacact atttgttgtt
     8161 aatagaacac tgctgtcact ttaacgactg acgattaaaa tagtttaatg agaataacgt
     8221 gaagttatgc gcttatatcg aacgaatagc tgagaattaa tcgtgataat gctttttcat
     8281 ttagcaaata cttcttccca gagctgaagc acaaccctgc gtgcatcgtt cagttgagtg
     8341 gcttcaatcc gtgcatatga aattgaagct tcttttgtag acgcagcacc tgtcaactcg
     8401 ggatccccac ttaatcgcaa gcgatgatgt atacggcgat attcccgata agttgataga
     8461 acatgctgtg ccatttcaga tggaatcaag ccgagattgc ctgcgagttt caataacgcg
     8521 atattcccga tattaccggt cagttgagga aactggtttg aataacctag cacgagatat
     8581 tgcaccataa actcgacatc gatgatccca ccacgatcat gtttgatatc gaataatgac
     8641 gtaggattgg gatgcgcatc gagcattttc tctcgcatca tcaaaatatc ttgcttaagc
     8701 ttggttaagt cacgtggctt acaaagtatt tccttacgga tggcttcaaa tgcagccccc
     8761 atttgttcat tccctactac aaagcgagca cgagtcaatg cttgatgctc ccatacccag
     8821 gcttgactct gttgataatg cttaaaagca tccaaagaac aagccaatag accactcgta
     8881 ccttcaggtc gcagccgtaa gtcggtttca tacaaaccac ccgcagaggt atgactcgtc
     8941 aaccaggtat taatactttg actcagcttg gtataaattt ccaatgcatc aggatgatta
     9001 tcatcataga gaaaaacgat gtccaaatct gaggaataac ctaattcctt accgcctaac
     9061 tttccatagc cgatgatcgc aaaaaccggc tgctcttgat gtttcttctt aagcccttgc
     9121 caagccaaat tcaatacatt ccccaaaata agatcagcga gctcagttaa gtgatcactc
     9181 agggtttcca gcaacaaaac accttccaga tcaactgcaa gcaactgaaa aacttgggca
     9241 tgttgaaagt gcctcaatac atccatttgc cactcagtaa tatttttctt aggactattg
     9301 atgtgattga gttgccgcat caattcaact tttagcgctt tccaatccgg tagttgataa
     9361 agtccctgag gcgctaacag ttcatcaagc aaaataggat gtcttcccaa ataattactc
     9421 gcccactgac tggaattcac taatttcgcg acccgcggta atgtttgagg atgttctaat
     9481 agcaaggcca aataagccgg atgctgactg atatgttcca gcaattgcag cattcgctct
     9541 agcgttaatt caataggtgg aaactgcgcc acagcttcaa ctaaagttgg catgaccgcc
     9601 gtaatttttt gttggctcgc taaaggcatt ttctgaaaaa attgactcac ataaaattgt
     9661 tgaatccgag cagaaatttt ttcgggatca ctaaacccta acgttgttaa ttggtgcgtt
     9721 gccgctttga cttgcatcaa atcactcggc tgttcttgcc agagcgcagt gagaatatcg
     9781 tgagtgggtg atttcctcgg tactgcaaaa atcaactcaa aatgccgtgt cacctggctg
     9841 cgatagatgt ccagcttagc aaataattca ggtggacttg caaaacccat agcggtagcg
     9901 atcaatgctt gatcatcggc attttcagga atcgattggg tttgctgatc gtctaaatat
     9961 tgcaatcgat gctcgagctt acgcaaaaat tgataagcat cgattaattc ttgaatagtc
    10021 tgagatggca aaggttgttt ctcgagtaaa cgacctaacg tgtccagtgt agggcgaata
    10081 cacaaatctg catcacgccc gcctcgaatc agctgaaata cttgcgtgat aaattcaatc
    10141 tcccgaattc caccggggcc cagtttaatg ttgtcatgca tttcgcgccg atcaacttcc
    10201 ttgcgcaatt gtgcatgaag tcgccgcatg gcttcatacg cttcaaaatc gagatatttc
    10261 cgaaaaacga atgggcgaac gatctgctcc atcaaaattg cttcttcagc caatgaacca
    10321 acaatcacgc gacttttgag ccaggcatgt cgttcccact cacgcccctg agtcgttaaa
    10381 tactcctcca acatcgcgaa actaatagta agtggactat tttcaccatg cggtcgtaac
    10441 cgcatatcaa cccgaaaaac gtaaccatct accgtgtagt cattcaagct ggcaatgagc
    10501 ttacgtccaa gtcgggtaaa gaactcatga ttcgaaattg atttttttcc attggtttca
    10561 ccttcttcag gataaagaaa aatgagatcg acgtcggaag agacatttaa ctcccctcct
    10621 cccaatttgc ccatcgcaat aactagtagt tgtcgagggg aagcactttt ttcgccaatc
    10681 ggctctccat aatgttcggg gtgagacatc caaatctggt gatgttttag cgcaaactga
    10741 atcgtgactt cagcaagttg agtcatggtc atcatgactt ctggcagacc tgccaaaccg
    10801 gccaaatcac ggactgctag ccgcaacatc actcgtttac gaagcttccg taatactgca
    10861 ttcagccccg cttcattcaa tgaaggagca acgcaagctg aattcaagaa atccagcatt
    10921 tcttctcgca gaaattggga tgcgccctta tccataaggt agccttgcaa atctggctca
    10981 ctcgctaaca atcgctgaac atagcgacta aaaggaagaa tcttttccag tagggactca
    11041 gttgtcgtgt gggatccgtg taaaatcatg gcaaaatagg cagcaaaatt cgctaactaa
    11101 gtatataaga attattgtca aaccagtcac gatgtatcac tcatcatgaa gtgtactttg
    11161 tatgattacg actgcaacac agctcatttt gctgtttcta cgttaaccca gttaagctaa
    11221 ctgtaattta taagaaactg aatactccat tgaatcaaca aaccccacgc caccaaccgt
    11281 atagccactc gattgcacga ctttctatcg tgtgtctatt tgctttagct gccctttttt
    11341 ctgtcaccat cctggcgcta cgttattgga ttttgccaaa tattgataat taccgattgg
    11401 aaattgcgca gattatctca aaaagggcgg aacaactcgt gagcatcgat cgaattaatg
    11461 cccattggaa tggcgtacac ccttaccttc agcttcatgg ggtaaaagtc tacgaccaag
    11521 cgggtacccc tgctttgatc ttaactgaag tagaaggaac gctttcatgg cattctatcc
    11581 ttcatcaaga gctgcgtttt cgcgaaataa aaattgatcg cccaacgcta attatgcggc
    11641 gtgacgcaca taatgtgatt catatcgccg gcactaacat ttatgacgaa aaaaacgata
    11701 cagaaaatag ttttatcgat tggctgttgc gccaggaaaa acttgtaatc aatcaaaccg
    11761 atatctattg gcttgatgaa taccgcaatg cgccggcgct gtacctcaaa attgtcaatc
    11821 tactactgca tagccataac ttaaccaatc ggcatcaatt aggcttaaga gttgctgcct
    11881 tggatagtac agcgatgtcg atggatattc ggggagactt tacaggtaaa tcgattgagt
    11941 cctttttgca ttggcaaggg cgcctctact ttgaattaaa acaaatcgat ttagccgttt
    12001 ggcgtcaatg gattccattt cctgatgaaa tcgatatcag acaaggcaaa ggtgccctaa
    12061 gaggttggtt aacgctagct gaaggtacaa ttactgactg gctaattgat accgctttac
    12121 atcaaatcag cgcaaaccta ggcacaaact tacccaaact cgaactcgat ttcttgcaag
    12181 gacgtattga cgtcaagcaa tccaatcaat ccggaagctt agtccaagaa tggacggcac
    12241 accaattaaa cttcaaatta gcgcatcaac aaatcataac aaacccaatc aatgcgatat
    12301 ggcgtcaata tcacgatcca aaacaaagtg aatcctcaaa ttatctacaa atcgacgaaa
    12361 tcgacgttgg agtgtgggct aatttacttg agtatttgcc ggtagatgca tcagtgcgta
    12421 agcaagttga aaagtactca ccgcatggaa aaattgaaca catgcagctc agttggaaag
    12481 gaagttggtc agaaccacac gcatatcaac tcgatacaca attcaatgat ttatccttaa
    12541 ataacattca gataggacat ttaatctcaa tccatgggct cagtggaaac attcaaggga
    12601 ctgagcaaga agggagactc aatttaaatt cccagtccgt ctctttgcaa ttacgacagc
    12661 cgtttccaca atcgattgta tttgatacct tagccacaag agtaggttgg aagaaaatag
    12721 cagaacaaga aagtaccact tacaaaatcg aagacctagc atttaacaac caacatttag
    12781 caggatccgc cattcgtggc agctatgtac acaaacaaaa taagcctgac tggattgata
    12841 tacaaggcaa tttaaaccag gccgacgtta acggtctctc tcgcttatta ttacctttca
    12901 ctaacaagac tcaactagcc tggatcgatg agtcatcatt atcagggcaa ttaaaaaata
    12961 cgcaatttaa tatccaaggt aatttaagcg attcttccgc aaacgatttt tcctggtcat
    13021 ttgctaccga aatggtcaat atatcagcta gtattggagc gcaatggcca agcattacca
    13081 atatttcagg gcacttgacg gctagctcta cactgattaa tgcaattgtg tcagaaggca
    13141 atatcgaaac gattcagttc aaagatactt ccatccatat cgctgatctt cagtcaaaaa
    13201 agccgcttct aaaaataaaa ggcgaagcaa caggaaaaac aaaacaaatc atccaattga
    13261 taaaagaatc accactaacg cagaatacat ttgatatcga tattccaggc gtgataactg
    13321 gcaagggaaa gttacaattc gaatcaacca tccccatcct gtcaaaagaa aaaattgttg
    13381 atatcgatct taaaggtcag tatcaattta tcaacaatca aatcgatttg ggtcccgagc
    13441 tacccgctat cagtaaagtc aatggaaagg tttcctttac tcataacaca tgggattttg
    13501 atgaaattca cgcacaactt ctgggcgggc cattagtaat ccgctctaag cctctcaaca
    13561 atgataaaaa acaaattgcc ttagaaggtc aggcaaattt cgaccgcttc aatacacgcc
    13621 tatcccaaaa atcaccgagt gctttgcagt tttggctaca gcatattcac ggcacgactg
    13681 actggacagc gatcatcgat atgaacaata gtcaaataac ggccctgcag atagcatcat
    13741 cactaaacgg tgttacttcg gtattaccag ctcctttttc taaaacacac gatgaaataa
    13801 ttccactcac ttttactaaa accgcgatcg actcagcaaa cgaaaagtta ttttgccaat
    13861 taggagaaaa agtcaccact gagattcacc gcacccagac aaaacaggga gatttcattc
    13921 cggtgcgtgg tgtcatgagt tttggcaatc gttcagcaac gttacccagc gaaaaattga
    13981 cattaatgca tggccctatt cctttactcg agtgggataa atggcaagaa atgcttgatc
    14041 aacacgaagc gatcgttggg ctgtcaaagc agcaaagtcg cggtatcgat gctttactca
    14101 cacatcacat acggtttgat ttaaatgtgg gggtccttga tttcttagga agtcgattca
    14161 atgattttat tttacatgcg gataaacagg gttcagagtg gactaccgac gtgacaagtc
    14221 gagaagttac cgggaaaatc tactggaatg cgacaggcaa aaaaaaggca accgcgaggt
    14281 taaaaaaatt agttgtacct gaatctcttc cggaaaacgg ttcagccgag agaaaaaagc
    14341 acgagtctca gaattggccg attatcgact tacatgcaga tgaatttttt tacaaagaaa
    14401 agctactcgg tcaattaacg ttattagccg agcaaaacaa agaaaattgg gtagttgatc
    14461 aaatggaaat ctcccacaag cacagtatct tcactgccaa aggcatctgg aaaaaccggc
    14521 aggaaccttt ttcaatgtat gcggagatca aattaacgac gagcgatatc ggcaaattcc
    14581 tcaaccgcgt tggttacccc gatcgcattg ctcgtggcga aggaagcttg gagggccacc
    14641 tgtcatggca agggaaacca ttctcagttg atttccccac cttatcaggg caactgaagc
    14701 tagctgctaa gcatgggcaa tttactaaat tcaaacccgg catgagcaag ttattaggca
    14761 ttttcgatct caaagcactg ccacggagac tgacacttga tttttatgat attttcagtc
    14821 aaggttttgg tttcgatgat attcttggaa atgttagaat tgatcacggg atcgcaacct
    14881 ttgtagattt agaaattgca gggagctctg cctatctcgc cgtttcagga gaaattaacc
    14941 ttgatcagga aacacaatca ttgcggatta aaatgtttcc atcgttgggg ttagtcacac
    15001 cggtgatcgg aatcgcttca atgattgcag atctttcact caaagatcca tttggaagag
    15061 tgatgtttaa tgaatatgct ataacaggaa catgggatga accgctggtc aagaaactat
    15121 ctgaggaaaa ctaataaaac atgaatacgc tacaagctat atctgctgtt ttgggagtca
    15181 gaatgtgtta aacagcacaa attctttttc ttcatcaaat tcagataaag aagaaaactc
    15241 caaaatacgg gttgcagcaa tccaaatggc ttccggcccc agcgtctcgg caaatttgga
    15301 ggaagcgctg cgcctaattg aactcgcagt cacacaacaa gcaaaactcg ttgtactacc
    15361 cgaatacttt tgcatcatgg gaatgcaaga cacggataaa cttaaagtcc gcgaaaagcc
    15421 gggggaagga gtaatacaaa cctttttgag cgaaactgcc aaacggttcc gaatatggct
    15481 tattggtgga tcagttccgc tcatctccca aatacccgat aaaattttta atagctgctt
    15541 agtttataat gatgccggcg aacaagttgc ccgctatgac aagattcatt tatttggttt
    15601 ctcacacggt aatgaacgtt ttgcagaaga aaaaaccatt aaagcgggtg ataaagttat
    15661 tgcaattgac tccccatttg gtcgaattgg gctttcaatc tgctacgatc taagatttcc
    15721 tgagctctac cgaatgatgg ggaagattga tatcatttta gctccgtccg cttttactgc
    15781 cattaccgga aaagcacatt gggagacatt aattcgcgca cgcgccattg aaaaccaggc
    15841 ttatgtgatt gcacctgcgc aaggtggata tcatgtaaat ggacgcgaaa ctaacggaga
    15901 tagcatgatc gtcgaccctt ggggtggaat aatgaaccgc ttatcccgcg gcccaggtgt
    15961 tgttgtcgca gctatcgatc ctgcttatca ggccagcgta agaacaagct tgccagcgct
    16021 tgagcatcgc tgtctctatc cacaatagtt caaagcgtga atctattaat acaaagtaaa
    16081 gcagtaataa aaaatactag ataacatggt aaatcataaa acggatatgc aaatagatag
    16141 agaagaacac ttctctattg cagaacgctg cctactaact ccatttcagc tggataccca
    16201 tcggctagat caagtatttg cagcaatgct atcgcaccat attgattatg ctgatcttta
    16261 ttttcagcat aatcgaaccg agggttgggt tcttgaagaa ggtattgtta agtcgggcag
    16321 ctttaatatt gatcaaggtg ttggtgttcg cgctctaagt ggcgaaaaaa cagcctttgc
    16381 gtattccgat gatatcagta tctctgccct acactctgcg gccaaagcaa ctcgtgccat
    16441 cgcaaatcag ggaaattcat cttctgcgcg aatcattact cacaataacg atcatcatcc
    16501 tttgctttac ttaccgcatg atccgatcac aagcataacc gatagcaata aggtccaatt
    16561 acttgaaaaa ctggaaagat acgcacgtac cctcgataat cgcattgtcc aagtattagc
    16621 ctccttatcc ggtgaatatg aggtcatcat ggtaattcgt agcgatggta agcttgtcgc
    16681 tgatgtaaga ccacttgtac gagtctccct tcaagtaatt gccgaagaaa atggaaggcg
    16741 tgaacaaggt gttgcagggg gaggcggtcg tttcgattac tcctatttca cagatgaaat
    16801 attgagcaac tatgcacaac aagccgtgca tcaagcaatc atcaatttga gtgcaaaacc
    16861 tgcgccggct ggtacaatga cggttgtgct tggtagtgga tggcctggta tcttattaca
    16921 tgaggcaatt ggtcatggat tagaaggcga ttttaaccgt aagggtagct ctgctttttc
    16981 tgaccgcatt ggagaacgtg ttgctgcaga tggtgtgaca gtcgttgacg atggaaccct
    17041 ggtcaatcga agaggatcgc ttaccgtaga tgatgaggga aatcctgctc aaaatacggt
    17101 tcttattgaa aatggggtac tccgaggtta tttacaagat agcctaaatg ctcgattgat
    17161 gaagcaaccc cttaccggca atggaaggcg cgaatccttt gcgcacatcc ctatgccacg
    17221 tatgaccaat acttatatgt taaatggcga taaagcccct gaagaaatta tcgcctcagt
    17281 taagcaaggc ctatacgcgg tcaattttgg aggggggcag gttgacatta cgagtggaaa
    17341 atttgttttt tctgctgcgg aagcgtatat gatagaaaat ggaaaaatca cctatccggt
    17401 aaaaggagca accttaattg gaaatggtcc agatgttctc acacgtgttt cgatgatcgg
    17461 gaatgatttg tcgcttgatc ctggtattgg aacttgtgga aaagaaggtc aaagtgttcc
    17521 agtaggcgtt gggcaaccca caatgcgtat tgatggatta actgtaggtg gcaccggcaa
    17581 ctaggtagta aagtaaattc taattttaca tttataatca gctttttaat gaagcaaccc
    17641 taagaaaaat atcaataata ggatgcaata ggaatgaccg aagaattaac tggtgcagaa
    17701 attacgatcc gttgcctaca ggaagaaggg gtagagtaca tttttggcta ccctggcggt
    17761 gctgtattat ttatttatga tgagctattt aaacaagata aagttaaaca tatcttggtc
    17821 aggcacgaac aagcggcatt gcatgctgcc gatgggtatg cacgatctag tcataaagtg
    17881 ggtgttgcac ttgtaacgtc aggaccgggt gtaaccaatg cggttacagg aatcgccacc
    17941 gcctatatgg attcaattcc attagtcatc atcagtggtc aagtaccaac tgctgccatt
    18001 ggacaagatg ccttccaaga agttgacacg gttggaatca ctcgcccgtg tgtcaaacat
    18061 aatttcttag ttaaagatat tactgagctt gctgccacga taaaaaaagc tttttttatt
    18121 gcttccagtg gaagaccagg ccctgttctc gtggatattc ctaaagatgt gacacagcaa
    18181 aaaacacact ttagctaccc gaatagtatt cacatgcggt cttataatcc cgtcaccaat
    18241 ggcaatacaa aacagataaa gaaagcagct gagcttatcc ttagcgcgaa acgccctatg
    18301 ttctattcgg gtggcggtgt cattcttgat aatgcagctg gtgtattgac tgaattagta
    18361 caactgctgg actatccctg tacaaataca ttaatggggc tggggggatt tccagcaacg
    18421 gataagcatt ccgtaggaat gctcggcatg catggcactt atgaggccaa tatgagcatg
    18481 cagtattgtg atgttttgat tgctgttggc gcacgttttg atgatcgagt cattggtaac
    18541 cctaaacact tttacagcga agatcggaaa atcattcata tcgatattga tccttcctcg
    18601 atttctaaac gcgtgaaagt agatataccg attgttggta gtgtttctca agtattaaaa
    18661 gaactcatta aactcattaa ggccagtaaa gagaaaacag atgccattgc attaaaagaa
    18721 tggtggaaac aaatcaatct gtggcgtgag cgtgattgct taaaatacga tcgttcaagt
    18781 catgtcatta aaccgcaaat ggtcattgag aaactctatg cgatcacaaa aggagatgcg
    18841 tttatcacct cagatgtggg tcagcatcaa atgtgggctg ctcaattcta tccgtttgat
    18901 aagccacgcc ggtggattaa ttcaggtgga ttaggtacga tgggatttgg cttaccttcc
    18961 gcgatggggg ttcaatttgc caaccctacg ggaacagtcg cttgcataac aggggaagca
    19021 agcattcaaa tgtgtatcca agaattatcg acttgcaaac aataccaact cccattgaaa
    19081 atcatcaacc ttaataatcg gtatatgggc atggttcgac aatggcaaga attttttcat
    19141 ggcaatcgct actcagaatc ttatatggat gcactaccgg attttgtaaa gcttgctgaa
    19201 agctacgggc atgttggtat gaaaattgag aagcctggtg atattgaagg tgctttgaag
    19261 gacgcgttta aactaaaaaa acgcctcgtc tttctagatt ttattacgga tcaaacagaa
    19321 aatgtattcc cgatggtgcc tggtggtaaa ggtttatcag aaatgatttt ggtataaaaa
    19381 atgcgacata ttatttcttt attgatggaa aacgaagcag gcacgctatc gcgagtggct
    19441 ggattatttt ctgcacgcgg ttataacatt gaatcactca atgttgcgcc gacggaagat
    19501 cccactttat cgcgaatgac attggttacg attggatctg atgaagtcat tgaacagatt
    19561 actaaacaac tcaacaaatt aattgaagtt gttaaaataa ttgacctcac agaaggcgat
    19621 catattgaac gcgaattaat gttagtaaaa gtaaaagcaa tcggggatgg gcgagacgaa
    19681 ataaaacggc taacggatat cttccgcgga agtatcatcg atgtaaccga taagtcctat
    19741 accattgaat taactggaac
//
LOCUS       DAOQKQ010000028        19541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_1233_length_19541_cov_6.259006, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000028 DAOQKQ010000000
VERSION     DAOQKQ010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 19541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19541
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1233_length_19541_cov_6.259006"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1448
                     /locus_tag="PKZ49_03065"
     CDS             <1..1448
                     /locus_tag="PKZ49_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HND35571.1"
                     /translation="APAYSLTGALGHGAAESGYQLPIVFILSVIPMYFVALSYKHLTT
                     AAPDAGTIFTWGTKAIGPRFGWLGGWALLLASILAGVAATQITVNAIIALMGLKDSPT
                     WLNLVIAILFILSTTYLTALGAKESSRTTMLLTAMQYGVLVVLAAILTLRVLQGDAVA
                     TANPFSLEWLNPFAIASFESFLGGFLIALFIFWGFDASLAMSEETAGTSEQIGRSGTT
                     AMMITVITYVVFSITALAYAGVDAQDPSSLTFEGNIDSIVTALATDTIGEMGAMIAAL
                     VIAISAFSATISTIMPAARVALAMATYRAIPAKFAYVSGVTKTPLVTTWTIGIMSLAI
                     YITLSSISESIVKDSIHSVSIAICTYYTVAALSCVFYFHRTAFNHWYHALSQVILPLI
                     AAVILIAVGIYQAWSMMDPNYGSSGSIAGVGAVFLIGGAALMLGVPLMILWNLREPRF
                     FQGKTLPLERAHMVLDSEDTKKNNSVLKVEK"
     gene            1445..2065
                     /locus_tag="PKZ49_03070"
     CDS             1445..2065
                     /locus_tag="PKZ49_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HND35572.1"
                     /translation="MSEYETLITSLALTMGASWASGINLYAVLLVLGIGSATNNIQLP
                     TELSVLENPFVIGAAGIMYTIEFFMDKIPGLDSVWDSIHTFIRIPAGALLAVGTVGDV
                     TPALEIAAGILGGSVAATSHATKAGTRLMLNTSPEPVSNWTASISGDFLVLGGLWTAL
                     NHPIFFLILFIGFIGFSIWFLPKLWRFIKILLQKIGTFFGLRGGQN"
     gene            2119..2574
                     /locus_tag="PKZ49_03075"
     CDS             2119..2574
                     /locus_tag="PKZ49_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35573.1"
                     /translation="MKFERLITAITNLAVVLGLVLVVFELQQNREAIQITHQLSLAGL
                     MSQIHLSVASDHELAELITRAKHDDTKNFSIAETERVNHWVLAILEPRISSYHLRSNE
                     FVVLEDWCNLMRDFTDLYRHPYFGNIVKSELSYADAIAADIENRCAKYR"
     gene            2646..3227
                     /locus_tag="PKZ49_03080"
     CDS             2646..3227
                     /locus_tag="PKZ49_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35574.1"
                     /translation="MTNTDWFTLTLVLITAFYAYATFKILRANEAMVTTMRDQQNAAM
                     RPYIIISANVRMGTQLFYLSIKNVGMTAAIDLSLSMDKNFYQFGEKREERNIANRVAF
                     SQTINSLPPDGQFLFLLGNGPTLYGESNSDELSPLLFTVTANYRSITDSYTETSVVDL
                     RPYINTDAPYDPIVEELRNLRQEVTKLAKLLEK"
     gene            complement(3275..3472)
                     /locus_tag="PKZ49_03085"
     CDS             complement(3275..3472)
                     /locus_tag="PKZ49_03085"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HND35575.1"
                     /translation="MLSPVLLELEQVKQHQGEILKQCATLFDNNKLTVSVTHTFSLSE
                     AATAQHFLEQNHPIGKLVLLA"
     gene            complement(3755..4030)
                     /locus_tag="PKZ49_03090"
     CDS             complement(3755..4030)
                     /locus_tag="PKZ49_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HND35576.1"
                     /translation="MKNNEPLPTAVALAYREGQTAPKVVAKGRGLIAEEIIHRAKEAG
                     IYVHESRDLVALLMQVDLDDRIPPQLYVAVAELLAWLYKLEHSKYSP"
     gene            complement(4020..5039)
                     /locus_tag="PKZ49_03095"
     CDS             complement(4020..5039)
                     /locus_tag="PKZ49_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HND35577.1"
                     /translation="MSPITAKPDLITPLTQIKSITPVSTDLDSQKNSSEFLPGQKYQA
                     LVDARLSNGNARVLIANQWFQMQLPENFQSGAKIELLVTSRLPELQFQIINGTQEQAS
                     DQPATISSTGKLISALLQDAHKQSTPTSQNTQISPSISSESSINSTELPSLLQKAISQ
                     SGLFYESHQAQWINGEKTLQSLHQEPQNKMNSTVMAAMDESVVRAVAASSNTMDVVVN
                     NQINTLVTQQLSTLETGQVVWRGEIWHNQPMEWEIYEQPEDHKQNEHEQTVQWHTKLH
                     LTMPNLGEITAKIAFGPHGLTIKLDAIDRNTIDLLKNNQQPLASQIQAAGLNVQSVEI
                     HQYEK"
     gene            complement(5137..5382)
                     /locus_tag="PKZ49_03100"
     CDS             complement(5137..5382)
                     /locus_tag="PKZ49_03100"
                     /inference="COORDINATES: protein motif:HMM:NF017237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HND35578.1"
                     /translation="MVAAKNGEWDLLISLEKECKHLTDLLKEKKPEPNLSDELLQEKI
                     EIIHQILEDDDQIRAITEPWMIRLQEILCANGYNRSL"
     gene            complement(5546..6541)
                     /locus_tag="PKZ49_03105"
     CDS             complement(5546..6541)
                     /locus_tag="PKZ49_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HND35579.1"
                     /translation="MKAVYFDKGGSADVLHYGDIATPNDCGENQVLVRIKAIGINPID
                     CKLRAAPERFPVTFPVIPGCDGAGIVEAVGTKVSHFKSGDEVYFSQPGFKGRQGTYAE
                     YTLVDASLLALKPRTLSFEQAVAAPLVFITAWEALHDRARIMHDQIVLIHAGAGGVGH
                     AAIQLAKLAGARVITTVSNEEKAAFVKQLGADLIINYRTQDVIAEVMRWTDSNGVDIA
                     FDTVGPTVLHSCFRCVKPYGDVVTILQATTDTDWSEARKRNVRFSHELMLSPVLLELE
                     QAKQHQGEILKQCAALFDNNKLTVSIVRTFKLSEAATAQHFLEHNHPAGKVILVV"
     gene            complement(6565..7065)
                     /locus_tag="PKZ49_03110"
     CDS             complement(6565..7065)
                     /locus_tag="PKZ49_03110"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HND35580.1"
                     /translation="MNPPIYLLAAVARNRVIGCDNKMPWHLPEDLKHFKQLTLGHIVI
                     MGRKTYESIGKPLPERINIVITRQPMFSAANLIVVASIEAALQACTPYPDKKRFVIGG
                     SEIYQQTLPMSQRLYLTEIQKDFVGNRYFPEFDRNEWSEAAREIHHQGTADGLEYHFV
                     ILDRRS"
     gene            complement(7062..7856)
                     /locus_tag="PKZ49_03115"
     CDS             complement(7062..7856)
                     /locus_tag="PKZ49_03115"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HND35581.1"
                     /translation="MHQYLELMRSILKQGHQKSDRTGTGTLSMFGYQMRFDLAAGFPL
                     VTTKKCHLKSIIYELLWFLRGDTNIHYLKQNGVSIWDEWADERGNLGPVYGHQWRSWT
                     VADGGAIDQISQVIEQIKKTPDSRRLIVSAWNVGDLDKMHLAPCHVLFQFYVVDGKLS
                     CQLYQRSADVFLGVPFNIASYALLTLMIAQVCNLKPGEFIHTFGDAHLYLNHLEQAQE
                     QLKRQPRQLPTMHIDPSVHRIFDFDYHHFELKDYDPYPAIKAPVAV"
     gene            7942..9411
                     /locus_tag="PKZ49_03120"
     CDS             7942..9411
                     /locus_tag="PKZ49_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HND35582.1"
                     /translation="MLATLIQNNTSINESVIASHVLVLLPKLEKLPNKYDVLGEETLK
                     ALLARRQMEFDRLSETPVCANLNNGVLCAWVMVDLEQPIFDQQTTIRKALQLLLDEEP
                     SSVHLAVNGNAHQKQSLARLAVYVAWVNGAVLPVRKNRPDRKPLDTIYLYGYEEADSF
                     TLLRAQAEGNLLARGLTVLPPNELTPKTYREQIKKLAQNESWNYQEYDLATLRKMGAG
                     AFVAVAQGSDTEDAAIVHLQRHSSKKHCKTIALVGKGICFDTGGHNLKSARYMHGMHE
                     DMNGSAVALGILLAATRTDLPFNIDCWLAIAQNHISPRAYKQNDVITAVNGTTIEIIH
                     TDAEGRLVLADTLALAEKLKPDLIIDFATLTGSMFTALGSRYSGIFTNRDYLAEHALA
                     AGKSSGERVCVFPMDKDYETELESNIADTKQCKLDSDFDHILAARFLSRFVNDIPWLH
                     MDLSACNHKNGLGAVGTDVTGFGVAWAAAFLEKLPKAEA"
     gene            9503..10324
                     /gene="amrB"
                     /locus_tag="PKZ49_03125"
     CDS             9503..10324
                     /gene="amrB"
                     /locus_tag="PKZ49_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HND35583.1"
                     /translation="MLTKRLPAVAGLFYPADCRTLGEEVNHYLRLTPISEIIPKALIV
                     PHAGYIYSGAIAAVGYNTLRSIAARIRLVVVLGPAHRVALRGLALPGVDSFMTPLGQV
                     EIDTDAVERIKNLPQVIVNAQAHESEHALEVQIPFLQTMLGEFKLLPLVVGMASTEEV
                     AEVLDCVWGGEETLIVISSDLSHYLPYESAQNKDSKTALSILQLRPLVNHEQACGAIP
                     INGLLAAARQHHLVAHLLDLRNSGDTAGSRDQVVGYGAFAFSEPHSPLARQYHVA"
     gene            10314..10892
                     /gene="amrA"
                     /locus_tag="PKZ49_03130"
     CDS             10314..10892
                     /gene="amrA"
                     /locus_tag="PKZ49_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HND35584.1"
                     /translation="MLLDQGNVLLAVGRAAIAQALNLDFQYPVDESASWLQAWGASFV
                     TLTQHQELRGCIGSLEAYRPLVADVRSNAVSAALHDPRFLPLSADEFETIAIEVSILS
                     LTQRIAALDEADAIRQMRPGIDGIVLEYGRYRSTFLPQVWDELEQPQQFLAMLKRKAG
                     LPVDFWAADLKLARYTVSKWRETDGMEDYRHG"
     gene            10885..11979
                     /gene="amrS"
                     /locus_tag="PKZ49_03135"
     CDS             10885..11979
                     /gene="amrS"
                     /locus_tag="PKZ49_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HND35585.1"
                     /translation="MDESISLEEQYPAKYWHQLQDSRVQCDLCPRDCKLHEGQRGACF
                     VRGRAGDRMVLTTYGRSSGFCVDPIEKKPLNHFYPGSSVLSFGTAGCNLACNFCQNWD
                     ISKSRSFDKLADQASPETIALTAKRLGCKSVAFTYNDPVIFAEYAMDVADACHAQGIN
                     TVAVTAGYIHAEPRADFFAKMDAANVDLKAFTESFYVKQTGSHIQPVLDTLKYLKHET
                     NVWLELTTLLIPGLNDSAQELQALITWVANELGSEVPLHFSAFHPDYKMRDIEATPLS
                     KLIEARRIAMAAGLRYVYTGNIHHPEGDTTYCSSCGRVLIERDWYEIKHYHLTANDHC
                     PDCGTAIAGRFGKSAEHFGRQRIPVLLGYQ"
     gene            11976..12620
                     /locus_tag="PKZ49_03140"
     CDS             11976..12620
                     /locus_tag="PKZ49_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HND35586.1"
                     /translation="MMTTISITDDTARLEGELMIPPGAQGIVVFVHGSGSSRFSPRNN
                     YVAKIIRDRGLGTLLFDLLTRQEDQNYTTRFEISLLTQRLLAATAWLGAETKTSKLKI
                     GYFGASTGAAAALQAAAKLGRKIAAVVSRGGRPDLAGVSALEKVMSPTLLLVGRADDG
                     VIELNEQAYSRLQCEKQLVLIPGATHLFEEPGTLEQVANQAADWFAHYLTDTAA"
     gene            complement(12623..13252)
                     /locus_tag="PKZ49_03145"
     CDS             complement(12623..13252)
                     /locus_tag="PKZ49_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HND35587.1"
                     /translation="MFRDRNDAANQLAKQLDHYRDLRPLILAIPRGAVPMGKMIADML
                     SGDLDVVLVRKLRAPGNPEFAIGSIDESGWTYLADYAGQFVHDSQYIEAEKAAQLKTM
                     QAQRKQYTPIRPPFDPENRIVIVIDDGLATGSTMIAALHTLKAKHPKKLICAVPVAPP
                     DTLEKIKPYADEIVCLNSPRDFYAVGQFYQDFPQVSDEEVIQSLKLSRH"
     gene            complement(13365..15851)
                     /locus_tag="PKZ49_03150"
     CDS             complement(13365..15851)
                     /locus_tag="PKZ49_03150"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HND35588.1"
                     /translation="MSKKLIIAEKPSVANDIAKVLGNFTKEADYFENDEYILSSAVGH
                     LLELAVPEEYEVKRGKWSFDHLPVIPPHFDLIPLEKTATRLKTLTKLIKRKDVDTLIN
                     ACDAGREGELIFRYIVRHSNTQKPIKRLWLQSMTPSAIKEGFNNLLNDSDLQPLAEAA
                     VSRSESDWLVGINGTRAMTAFNSQEGGFHKTTVGRVQTPTLAILVEREEAIKKFIPKN
                     YWELHATFSTNSHSYKGKWFDEKFTKSKENAEARAERIWDLANAEAIRLKCLGKSGTV
                     SEESKPAKETCPLLYDLTSLQREANSRFGFSAKSTLGLAQALYEKHKVLTYPRTDSRA
                     LPEDYLETVKETLQSLTASPYHEFASQILVSDWVKPNKRIFNNAKISDHFAIIPTSLE
                     PKKLNEAELKLYDLVTKRFLAIFYPSAEYLVTTRITRVENETFKTEGKILVNPGWQIV
                     YGKSSPSADSDQENLTLAAIGPNATVKTEEIDTIANQTRPPARFNESTLLSAMEGAGK
                     LVEDEELRAAMSSKGLGTPATRAAIIEGLVSEHYLLREGRELLPTAKAFSLITLLRGL
                     KIPELISPELTGDWEYKLRQIEQGQLKRQDFMNKIAEMTKHMVDQAKSHRGETISGDF
                     AVLKTPCPKCGGILHETYKKFQCQQCDFALWKILAGRQFEVDEMETLISQREVGPLLG
                     FRSKMGRLFTASIKLTDTFEMKFEFGNGDDLEAEPIDFSNHQLLGTCPQCNHHVYEHS
                     TNYVCEKAVGPSPTCNFKVGKVILNRVIEQEQIMKLLTHKKTDLLTNFISKKGRPFSA
                     YLVVNADNKIGFEFEERKPSKVRSKKTSKN"
     gene            complement(16021..16479)
                     /locus_tag="PKZ49_03155"
     CDS             complement(16021..16479)
                     /locus_tag="PKZ49_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HND35589.1"
                     /translation="MFEILIYLFENYFDSENCPDSETLTRKLTLAGFGEDEISQILGW
                     LTDHTKHEAGKYPVTLSQSKSFRIYSSSELEVIDVESQGFILFLEQAGVLNSLQRELL
                     IDRILQMDGSSSCLDKIKLVVMFDLWVQNQLTNHDLIEKLFIASNPRNRH"
     gene            complement(16537..17640)
                     /gene="dprA"
                     /locus_tag="PKZ49_03160"
     CDS             complement(16537..17640)
                     /gene="dprA"
                     /locus_tag="PKZ49_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HND35590.1"
                     /translation="MNIQPDIESWLTLSFIEGLGDESFRKLLLTFKHPTDILNAEAQL
                     LERYVKKPIAADIANRRVDQKKIIATLKWLEDPANAIITLNDDNYPKRLLNIADPPPL
                     LYLKGQRELLNTPSIAIVGSRNGTPQGIVNASAFAEAASSAGLCVVSGMALGIDTAAH
                     QGALLGSSSSIAVVGTGLDIVYPAKNHQLAHQLAKEGALISEFPLGMPAIGRNFPRRN
                     RIISGISLGCLVIEATLKSGSLITARQALEQGREVLAVPGSIHSPLSKGCHALIKQGA
                     KLVENINDILDEFNYQPLNLANPLATDEAIKQTYAQENTILLSHLDYEATDIDKLCIR
                     SGLTVETVSAMLLALELDGIVASLPGGRYQRIR"
     gene            complement(17675..18706)
                     /locus_tag="PKZ49_03165"
     CDS             complement(17675..18706)
                     /locus_tag="PKZ49_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HND35591.1"
                     /translation="MRTISIIAMILAISIFSDRAAKAKDIELLANAPERYVVSQGDTL
                     WGIASRYLKDPWQWTEIWDMNQKQIKNPNNIYPGDIIILVRAADGSSKLMFANEKATI
                     KLSPKMRIEKLEAEKIPSIPSKKLKPFLSQPLVIEKNGLDRAPLILGSSDNRVILSTG
                     NTVYVQNMPFDQGMAWQVFRAGKPLIDPEDNDRVLGHEAVYLGDVEVSNFDAISSVRI
                     THSVQEILKGDRLIPASTLHVDDYIPRAPDFPVTGRIISVYGGVTEIGQNAIIALNKG
                     SSDGLEVGHVLAVYRKSTAKLQDSKIISLPDERIGLVFLFRVFDKVSYALVLHSAQAI
                     KVFDAVQTP"
     gene            18827..19354
                     /gene="def"
                     /locus_tag="PKZ49_03170"
     CDS             18827..19354
                     /gene="def"
                     /locus_tag="PKZ49_03170"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HND35592.1"
                     /translation="MAILKILQYPDERLHKIASVVPAITRDTRVLVENMAETMYHASG
                     IGLAATQVNVHQRVIVIDTSETHDQLNVFINPEIITKAGDAEFQEGCLSLPGIFDKVK
                     RAELVTVRALNLQGESFEMQAEGLLAVCIQHEIDHLEGKVFVEYLSQLKQSRIQTKLK
                     KQQRLTKPSVLSMSV"
     gene            19377..>19541
                     /locus_tag="PKZ49_03175"
     CDS             19377..>19541
                     /locus_tag="PKZ49_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HND35593.1"
                     /translation="MRIVFAGTPAFAAIALKALIDAQFQIVLVLTQPDRPAGRGLKLQ
                     ESEVKKVASEY"
BASE COUNT         5142 a         4013 c         4570 g         5816 t
ORIGIN      
        1 ctgcaccagc ttattcgctg accggtgcat tggggcatgg tgctgcagaa agtggttatc
       61 agttgccgat tgtgttcatt ttgtcggtga ttccgatgta cttcgtggcg ttatcttata
      121 aacatctcac gaccgcagca ccggatgctg gaacgatttt tacctggggc actaaagcga
      181 tcggcccacg atttggctgg ctcggcggat gggcgttgtt attggcgagt attctcgcag
      241 gggttgctgc aactcaaatc acggtcaacg ccatcatcgc tttgatgggt ttaaaagata
      301 gccccacttg gttaaatctg gttatcgcca ttttgtttat tctcagcacc acttacttga
      361 cggcgctggg cgcgaaagag tcatcgagaa cgacgatgtt gctgactgcg atgcaatatg
      421 gtgtgttagt ggtgctcgcg gcaatattga cgctacgtgt tttgcaaggt gacgcggttg
      481 ctactgcgaa tcccttttcc ctggaatggt tgaacccgtt tgccattgct tcattcgagt
      541 catttctggg tggatttctg atcgcacttt tcattttctg gggatttgat gcttcgctgg
      601 ccatgtcaga agaaaccgca ggaacttcag agcaaatcgg tcgcagtggc actaccgcaa
      661 tgatgattac cgtcattacc tatgtggtat tttccattac cgcgttggcg tatgctggcg
      721 ttgatgctca ggacccatct agtttaacgt ttgaggggaa tatcgattcg attgttaccg
      781 cgttggcaac ggataccatc ggtgaaatgg gcgccatgat tgcagcattg gttatcgcga
      841 tttctgcatt ctcagcaacg atctcgacga tcatgcctgc agcgcgggtt gcacttgcga
      901 tggcaactta ccgggcgatt cctgcaaaat ttgcttacgt cagtggcgtc acaaaaacgc
      961 ctttagtgac gacctggacg ataggcatca tgtcgttagc gatctatatc acgttaagtt
     1021 caattagcga atcgatcgtc aaagattcta tccatagcgt tagcattgct atctgcactt
     1081 actacacggt tgcggcgcta tcttgtgtgt tttattttca ccgtacggcg tttaatcatt
     1141 ggtaccacgc tttgtcacaa gtcattttac ctttaatcgc agcggttatt ctgattgcgg
     1201 tgggaattta ccaagcttgg agtatgatgg atcccaatta tggttccagt ggttcgattg
     1261 ctggggtggg cgctgtattc ttgatcggcg gtgctgcatt aatgttaggt gttccgctga
     1321 tgattctttg gaatctgcgt gaaccgcgat ttttccaggg caagacatta ccccttgaaa
     1381 gagcgcatat ggttctcgat agcgaagata cgaagaaaaa taatagtgtg ttgaaggtgg
     1441 aaaaatgagt gaatatgaaa cgctgattac atcattagcc ctgacaatgg gcgcatcatg
     1501 ggcgagcggc attaatttgt atgcagtatt gttagtgctc ggcatcggta gcgcaaccaa
     1561 caatatacag ttgccaaccg agctttcggt attagagaat cctttcgtga tcggtgctgc
     1621 aggtatcatg tatacaattg agttcttcat ggacaaaata cctggattgg atagcgtttg
     1681 ggattcgatt cataccttta tccgtattcc agccggtgca ttactggcgg ttggcacagt
     1741 cggtgacgtt actccagcgc tggagattgc tgcaggtatt ttgggcggca gtgttgctgc
     1801 cactagccat gcaaccaaag cgggaacgcg gctgatgctc aatacttctc ccgagcctgt
     1861 atcaaattgg actgcatcaa tcagtgggga ttttttggta ttgggtggtt tatggaccgc
     1921 actcaatcat cccatctttt ttctgattct gtttattggt tttattggtt tctcgatttg
     1981 gtttttacct aaattgtggc gattcattaa aatcttgttg cagaagatag gaaccttctt
     2041 tggtttaagg ggcggtcaga attgaaagtc ttgatttctt ttacggtaag gcttacgagt
     2101 tgatagtggg taacatccat gaaatttgaa cgattgatca ccgcgattac aaatcttgct
     2161 gtggtattgg gcttagtgct tgttgtattt gagctgcagc aaaaccgtga agcaattcag
     2221 attacgcatc agttatcgct agcggggcta atgagccaaa ttcacttgtc ggttgcatcg
     2281 gatcacgaac tggcagagtt gatcactcga gcaaagcatg acgatacaaa gaatttcagt
     2341 attgcggaga ctgaacgcgt aaatcactgg gttttggcaa tccttgaacc acgcatcagt
     2401 tcataccatc tcagaagtaa cgaatttgtt gtcttggaag actggtgtaa tcttatgcgc
     2461 gattttacag atctttaccg gcatccctat ttcggcaata tagtaaagtc agaacttagt
     2521 tacgcagatg ccatcgcagc agatattgaa aatcggtgtg ctaagtatcg atgacgccta
     2581 tatttatcaa tgtcgatttg gggaagaaat aatcaaatct agttgctatg cttttacttg
     2641 aatccatgac gaatacagat tggtttacgt taacgctcgt acttattaca gcgttttatg
     2701 cgtacgctac tttcaaaatt cttcgcgcta atgaagcaat ggttacaact atgcgagacc
     2761 agcagaatgc cgctatgcga ccatatatca tcatatctgc aaatgtaaga atgggaactc
     2821 aactctttta tctatcgatt aagaatgttg gaatgactgc ggctattgac ttaagcctct
     2881 ctatggataa gaacttctac cagtttggag aaaaacgtga agagcgcaat attgcgaata
     2941 gagtagcttt ctcacaaacc atcaatagct taccacctga tggtcaattt ctatttttac
     3001 taggaaatgg gccaacctta tatggtgaat ctaatagtga tgagctttct ccgctattat
     3061 ttacagttac tgcaaactat agatctatca ctgattcata cacagagact tcggttgtgg
     3121 atcttcgtcc atatattaat accgacgccc cctatgatcc aattgtcgaa gagttgagaa
     3181 acttacgtca ggaagttact aagctcgcca agcttctaga aaaatagttt taatgtgaat
     3241 gggatctgga tatccttcag aggctcgatt attgttatgc caaaagtacc agctttccaa
     3301 tcggatgatt ctgctccaga aaatgttgtg ctgttgcagc ctctgaaagt gagaaagtat
     3361 gtgtaaccga tacagtgagc ttgttgttat caaacaatgt ggcacattgc tttaatattt
     3421 cgccctgatg ttgcttgact tgctctaact ccaacaaaac cggtgacagc atgaattaaa
     3481 acgatttgat cttgcgtgat tcttgcccaa tcatgcagtg cctcccacgc ggtgataaac
     3541 actaatggtg ccgcagctgc ttgttcaaag gataataagc gcagttttaa tgccagcaag
     3601 ctggaatcga ctactacaaa ctcggcgtaa gtgccttgtc ggccattaaa accgggttga
     3661 gaaaaataaa cttcatttcc aggtttgagg tcgctaacct ttgtaccaag tgcttctaca
     3721 atacctgcac catcgcagta atatcaccac tgatttaagg tgaatacttg ctatgttcca
     3781 gtttatatag ccaggcaaga agctcggcga cagcaacata aagctgcgga ggaatacggt
     3841 catcaagatc aacctgcatc aataatgcaa ccaagtctct cgattcatgg acatagattc
     3901 cggcttcttt ggcacgatgg ataatttctt cagcgatcaa acctcttccc ttggcgacca
     3961 ctttgggcgc agtttgacct tcccgataag cgagcgctac agcggtgggc agaggctcat
     4021 tatttttcat actgatggat ttcaacagac tgaacattca agccagctgc ttgtatttgc
     4081 gaggcgagag gctgttgatt atttttcagc agatctattg tgttcctatc gatggcatcc
     4141 agtttgatgg ttaatccatg gggtccgaat gcaattttgg ctgtaatctc acctaggttt
     4201 ggcatcgtga gatgtaattt cgtgtgccat tgaacagtct gctcgtgttc attttgcttg
     4261 tgatcctcag gttgctcata aatctcccac tccatgggtt ggttatgcca tatctcaccg
     4321 cgccaaacaa cctgtccggt ttccagtgtg gatagttgct gcgtcaccaa cgtgttgatc
     4381 tggttattca ccacaacatc catagtattg gaactagccg ctactgctct aactacggat
     4441 tcatccattg ctgccattac cgttgaattc attttgttct ggggttcttg gtgaagactt
     4501 tgcagtgtct tctctccatt aatccactgt gcttgatgag attcatagaa taatccactt
     4561 tgagaaattg ctttctgcaa taaacttggt agctcagtgc tgtttatgga agactctgat
     4621 gaaatggaag gagatatttg agtattttgt gaagtaggcg ttgattgctt gtgtgcatct
     4681 tgtagtagag cgctgatcaa tttcccagta gagctgatcg ttgcgggttg atcgcttgct
     4741 tgctcttgag ttccattaat aatttgaaac tggagttcgg gtaatcgcga tgtgactaat
     4801 agttctattt ttgcacccga ttggaaattt tcgggtaatt gcatctggaa ccactgattg
     4861 gcaatcagga cgcgtgcatt gccatttgat aatcgtgcat caacgagggc ctggtatttt
     4921 tgcccgggta ggaattcaga gctgtttttt tgtgaatcga ggtcggttga aacaggtgta
     4981 attgatttga tttgagttaa gggggtaatc agatcaggtt tggcagtaat cgggctcatg
     5041 atgattttcc cccttcaact cacatctttt ttcgttacaa agatgcatct attcgggttg
     5101 aaacgatcag atgttctcga atgattgata ggtttgttag agacttctat tgtatccatt
     5161 ggcacacagg atttcttgca gtcttatcat ccatggttca gtgatggctc tgatctgatc
     5221 gtcatcttcc aagatctgat ggataatttc gattttctct tgtaataact catcgcttaa
     5281 gtttggttct ggttttttct cttttagtaa atcggttaaa tgtttgcatt ctttctctaa
     5341 tgatataagc aaatcccatt caccattttt agcagcaacc agtatttttc ccgttgtttc
     5401 taaaatagct gtacggcaag gaatatcact tattcatgaa gatgagtgcg gttggagtga
     5461 cacaaactca gggtgaatta agaattaata aaaatcctat actgagcaga attgtcatgt
     5521 cagttcagcg tatctacgat tatttctaaa caactagaat cactttccca gctggatgat
     5581 tgtgctctag gaaatgctgc gctgttgcag cttctgagag tttaaaggta cgtacaatcg
     5641 atacagtaag cttattgtta tcaaacaatg cggcgcattg ctttaatatt tctccttgat
     5701 gttgcttggc ttgctctaac tccagtaaaa ccggtgaaag catgagttca tgactaaagc
     5761 gcacattgcg tttacgcgcc tcgctccaat cggtatcagt cgtggcttgt agaatagtga
     5821 cgacatcgcc gtaaggtttg acgcaacgga agcaactgtg caggactgtt ggaccaacgg
     5881 tatcgaaggc aatgtctacg ccgttgctat cagtccagcg catgacttca gcaataacgt
     5941 cttgggtacg gtagttaata atcaaatcgg cgccgagctg tttcacgaaa gcggcttttt
     6001 cctcattgct gacggtggtg ataacgcgag cgccagctag tttagctagc tgaatcgcgg
     6061 catggccaac accaccggcg ccagcatgaa ttaaaacgat ttgatcgtgc atgatccttg
     6121 cccgatcatg tagtgcctcc cacgcggtga taaatactaa tggcgcggcg accgcctgtt
     6181 caaaagataa cgtacgcggc ttcaacgcaa gtaaactggc atcgactaag gtatactcag
     6241 cataggttcc ttggcgacct ttaaaaccgg gctgtgaaaa ataaacttca tcgcctgact
     6301 tgaaatggct gaccttggtg ccgacggctt ctacaattcc tgcaccatcg caaccgggta
     6361 tcacgggaaa agttacgggg aaacgttcgg gtgctgcacg gagtttgcag tcgatgggat
     6421 tgattccaat cgctttgata cgaaccagta cttgattctc accgcagtcg tttggggttg
     6481 cgatatcacc gtagtgcaat acgtcggctg accctccttt gtcaaaataa actgctttca
     6541 taattggatt tccataaaat gactttaact tcttcgatca aggattacaa aatggtactc
     6601 gagtccatct gccgtgcctt gatggtggat ttcacgcgct gcttcagacc attcattgcg
     6661 gtcgaattcg ggaaagtagc gatttcccac aaaatctttc tgaatctcgg taaggtagag
     6721 gcgttgactc ataggaagtg tttgctggta aatttctgaa ccaccaatca caaagcgttt
     6781 tttatcgggg tagggtgtgc aggcttgcaa tgcagcttcg atggatgcaa cgacgatcaa
     6841 gttagcagcc gagaacatgg gttgtcgcgt aatcacgata ttgattcgct ctggtaaggg
     6901 tttgccgatc gattcatagg tttttcgccc catgatgacg atatgcccca gtgtcagttg
     6961 cttaaaatgt ttcaagtctt ctggaagatg ccatggcatc ttattatcgc aaccaatgac
     7021 ccgattacgg gcaacggcgg ctaataagta aataggggga ttcatactgc cacgggcgct
     7081 ttaatcgcag gatagggatc atagtctttc aactcaaaat ggtgatagtc aaagtcaaaa
     7141 atacgatgaa ctgatgggtc aatgtgcatg gtcggcaatt gtcttggttg tcgtttgagc
     7201 tgttcttgcg cttgttccag atgattaagg taaagatggg catcgccaaa ggtatgaatg
     7261 aattcgccag gttttaggtt gcacacttga gcaatcatta gcgtcaacaa cgcataagaa
     7321 gcaatattga aaggaacgcc taaaaaaaca tccgcactgc gttgatagag ttggcaagat
     7381 aacttgccat ccacaacata aaactggaaa agtacatggc aaggcgctaa gtgcatttta
     7441 tccaagtcac ccacgttcca agcggataca atgagacgcc gcgaatcggg tgtttttttg
     7501 atttgttcga tcacttgcga gatttgatca atcgcgcctc catcggcaac tgtccatgaa
     7561 cgccattgat ggccatagac cgggccgaga ttgcctcgct catccgccca ctcatcccag
     7621 attgaaacgc cattttgctt cagatagtgg atattggtat cgccgcgcag aaaccataag
     7681 agctcataga taatggattt caagtgacac tttttcgtgg tgaccaaagg aaatcctgct
     7741 gcgagatcaa atcgcatttg gtagccaaac atggacaagg tgccggttcc agttctgtcc
     7801 gatttctggt ggccttgctt aaggatagaa cgcattaatt caagatattg gtgcataggt
     7861 ggtgagtata cttctgagaa gaaaaaacca ggtagcccct tataaagcta taataaggac
     7921 aacttaatga taggaattct gatgcttgca acactcatac aaaataatac atccattaac
     7981 gaatctgtaa tagcttctca tgttttagta ctattgccaa aactcgaaaa attacctaat
     8041 aaatatgatg tattgggaga agaaacacta aaggcactgc ttgctcggcg tcaaatggag
     8101 tttgatcgac tgagtgaaac gccggtatgc gctaatctga acaatggtgt gttgtgtgcc
     8161 tgggttatgg tggatttgga acaaccaata ttcgatcaac aaaccacgat tcgaaaagcc
     8221 ttacaactat tgctggatga agagccaagc tcggttcacc tggcagtaaa tggaaatgct
     8281 caccagaaac aatcactggc taggttagcg gtgtatgttg cttgggtgaa tggtgctgtt
     8341 ttaccggtac gcaaaaatag gcctgatcga aaaccactgg atacgattta tttatatggg
     8401 tacgaagaag cggatagctt cacgttgctc cgtgctcaag cagaagggaa tttgttagcg
     8461 agagggttaa cggttttacc gccgaatgaa ttaacaccca aaacgtatcg cgagcagatt
     8521 aagaaattag cccaaaatga aagttggaac taccaagaat atgacctagc gactctccgt
     8581 aaaatgggcg caggtgcatt tgtggcggtt gctcagggta gcgataccga agatgctgca
     8641 atcgtacatt tgcagcgaca cagttcaaag aaacattgca aaacgatcgc attggtcggt
     8701 aaggggatat gctttgatac gggagggcac aacctaaaaa gtgcgcggta tatgcacggt
     8761 atgcatgagg atatgaatgg ctcggctgta gcgttaggca ttttattagc agcgacccgg
     8821 actgatttac cgtttaacat cgattgctgg ttagcaattg ctcaaaatca catcagcccc
     8881 cgcgcctata agcaaaatga tgtgattacc gcagtcaatg ggacgacaat cgaaatcatt
     8941 cataccgatg cagaaggacg cttggttctc gcagatacct tagctttggc cgaaaaactc
     9001 aaacctgatt taattatcga ttttgcaaca ttgacgggga gtatgtttac cgcgctagga
     9061 tcacgctata gcggtatttt cacgaatcgc gattatttag cggaacacgc attagcggct
     9121 ggtaaatcaa gcggggaacg tgtgtgtgta tttccgatgg ataaggatta tgaaacagag
     9181 ttggaaagta atattgctga caccaaacaa tgtaaattag acagcgattt tgatcatatt
     9241 ctcgctgctc gctttttgag tcgttttgtc aacgatatac catggctcca tatggatctt
     9301 tcagcctgca atcataaaaa tggtttgggc gctgtcggga ccgatgtgac tggatttgga
     9361 gtggcttggg cagccgcgtt cctggagaaa ttaccgaaag ctgaggcgta gataacttcg
     9421 gttttattct aaagttttat tattgccctt gtttttctga tacatattcc catattgata
     9481 tcgttcaata cagggaaggt ttatgttgac aaaacgattg cctgctgtgg caggcttgtt
     9541 ttatccagcg gattgtcgca cattaggtga agaagttaac cactatttaa ggttgacacc
     9601 tatctcagaa ataataccta aggctttgat tgttccccac gcaggttata tttactcagg
     9661 cgcgattgca gcagtcggtt acaacacgtt gcgctcaatt gcagcgcgca ttcggctcgt
     9721 tgtagtgtta gggccagcgc atcgggttgc tttgcgagga ttagcattac ccggtgtcga
     9781 tagttttatg acgcctttag gtcaggttga gattgatact gacgcagttg agcgtataaa
     9841 aaacttaccc caagttatcg tcaatgccca agcgcatgag tcggagcatg cattggaagt
     9901 gcagatccct tttttacaaa cgatgcttgg tgagtttaaa ttattaccat tagtggttgg
     9961 gatggcatcc accgaggagg ttgctgaggt attggattgt gtctggggtg gagaagaaac
    10021 actgattgta atcagctcag atctttctca ttacttacct tatgagtcgg cccagaataa
    10081 agacagtaaa acagcgctat caattcttca gttacgaccc ctggttaatc atgagcaagc
    10141 atgcggcgcg atcccgatca acggcctgct tgcagctgcg cgtcaacatc atttggtagc
    10201 acatctattg gatttgcgaa attcgggcga taccgctggg tcgcgcgacc aagttgttgg
    10261 ctatggtgct tttgcattct ccgaaccaca ttctccatta gcgaggcagt atcatgttgc
    10321 ttgatcaagg gaacgtgtta ttagcagttg gacgtgctgc gatcgcacag gcattgaatc
    10381 tcgattttca ataccctgtt gacgagtcag cgtcttggtt gcaagcctgg ggtgcgagtt
    10441 ttgttacgct gactcagcat caagaattgc gcgggtgtat tggttcgctc gaagcctatc
    10501 gacctctagt agcagatgtt agaagtaacg cggtatcggc cgcattgcat gatccccgtt
    10561 ttttaccttt gagtgcagac gagtttgaaa cgattgcgat tgaggtttca attttatctc
    10621 taacgcagcg aatcgctgct ctggatgaag cggatgcgat aagacaaatg agaccgggta
    10681 tcgatgggat cgtgcttgag tatgggcgct accgcagtac ttttttaccc caggtttggg
    10741 atgaacttga acaaccccag caatttttag cgatgctaaa acgcaaagcg ggcttaccgg
    10801 ttgatttctg ggctgctgat ttaaagcttg ctcgttatac ggttagcaaa tggcgtgaaa
    10861 ctgatggcat ggaggattat cggcatggat gagtccatta gccttgagga acaatatcca
    10921 gctaaatatt ggcatcaatt gcaagatagc cgtgttcagt gcgatctgtg tcctcgggat
    10981 tgtaaattac atgaagggca gcgtggtgct tgttttgtca gaggaagagc tggggatcgg
    11041 atggtgctga cgacctatgg acgatcatct ggcttctgtg tggatcccat tgagaaaaaa
    11101 ccgcttaacc atttttatcc gggcagtagt gtcctatcgt ttggtactgc aggttgtaac
    11161 ctggcttgca acttttgcca gaattgggat atttctaagt cgcgtagttt tgataaatta
    11221 gctgatcaag caagtcctga aaccattgcg ctaaccgcta aacgcctggg ttgtaaaagt
    11281 gttgcattta cctacaatga tccagtgatt tttgctgaat atgcaatgga tgtcgcggat
    11341 gcttgtcatg cacagggcat caatacggtt gcggttacgg caggttatat ccacgctgaa
    11401 cccagagctg atttttttgc caagatggat gcggccaatg tcgatctcaa agcgtttact
    11461 gaatccttct acgtgaaaca aactggttcc catatccaac ctgtgcttga tacattgaaa
    11521 tatctcaaac acgaaacgaa tgtgtggctt gagctaacca ctttactgat accaggattg
    11581 aacgattcgg cgcaagaact ccaggcattg atcacatggg tagccaatga attgggtagc
    11641 gaagtcccct tgcatttctc ggcttttcac ccggattaca aaatgagaga tattgaggca
    11701 actccgctca gtaagttaat tgaggcgaga cgaattgcga tggcggctgg cctccgctat
    11761 gtttataccg gaaatatcca tcaccctgaa ggcgacacga cgtattgctc atcgtgtggt
    11821 cgcgtgttaa ttgagcggga ttggtatgaa attaagcact atcatttaac tgcaaatgat
    11881 cattgccccg attgtggtac tgcaattgct gggcgtttcg gaaaatctgc tgaacatttt
    11941 ggtcggcagc gcatccctgt tttattagga tatcaatgat gacgacgata tcgattaccg
    12001 atgatacagc acgattagag ggtgaattaa tgattcctcc aggcgcgcaa gggatcgtgg
    12061 tgtttgtgca tggcagtggt agcagccgat ttagtccgcg taataattat gtggcaaaaa
    12121 tcattcgaga tcgtgggtta ggaacattgc tgtttgatct gttgactcga caggaggacc
    12181 agaattatac aacccgtttt gagatcagct tgttgacgca acgtttgctt gcggcaactg
    12241 cttggcttgg agcagaaact aaaaccagca agctaaaaat cggatacttt ggcgccagta
    12301 cgggtgctgc tgcagcttta caagcggctg ccaagctcgg aagaaaaatt gcagccgtgg
    12361 tatcacgagg aggacgtcct gatcttgctg gtgtgtcagc gttagaaaaa gtgatgtcac
    12421 ctaccttact tttagttggc agggcggacg atggggtaat cgaattaaac gagcaggcct
    12481 attcacggtt acagtgtgaa aagcaactag tattaatacc gggagcaact catttgtttg
    12541 aggaacctgg gaccttagaa caagtggcaa atcaggctgc agattggttc gctcattatt
    12601 taaccgatac cgcagcatga gattaatggc gactgagctt taaggattga atgacctctt
    12661 cgtcgctgac ctgcggaaag tcttgataaa actgaccaac tgcgtagaaa tcacgcggag
    12721 aattgagaca aacgatttcg tcagcgtagg gttttatttt ttctaaggta tcgggtggtg
    12781 cgacgggtac agcacagatt agtttttttg gatgtttggc ctttaatgta tgtaatgctg
    12841 caatcatggt ggatcctgta gccaaaccat catcgatcac aattacgatc cgattttctg
    12901 gatcgaatgg cgggcgaatt ggggtatatt gcttgcgttg cgcttgcatc gtttttaact
    12961 gagcagcttt ttccgcttca atatactgag aatcgtgcac aaattgtccc gcgtaatctg
    13021 ctagataggt ccaaccgctc tcatcaatgg aaccgatagc aaactcgggg ttacctggtg
    13081 ctcgcaactt cctgacaaga actacgtcca aatcacctga gagcatatca gcaatcattt
    13141 ttcccatcgg aacggcaccg cgtggaatcg ctaaaatgag cgggcgaaga tcgcgataat
    13201 gatcgagttg tttagctaat tggtttgctg catcattacg atcacggaac attttgaatc
    13261 aaatcgcttg gtcgcgttat tgaatgaact gtttacgatt gaatttccta actaagtata
    13321 acttaaagaa atagtgtgag ggttaggtat tcattctgag aaattcaatt cttgcttgtt
    13381 ttcttacttc gtacttttga tggttttctt tcctcgaatt caaaaccaat tttgttatca
    13441 gcgttaacta caagataggc agagaaaggt cggccttttt tggagatgaa attcgttaat
    13501 aaatctgttt ttttatgtgt aagtaatttc attatctgtt cttgctcaat cacacgattg
    13561 agaatgactt tcccgacttt gaaattgcag gttgggctag ggccaactgc cttctcacag
    13621 acgtagttgg tgctatgttc atagacgtga tgattgcatt gagggcatgt tcccagtaat
    13681 tgatgattgg agaaatcaat tggttctgct tctaaatcgt caccattgcc aaattcaaac
    13741 ttcatttcaa aggtatcagt gagtttgatg ctggcagtga ataaacgtcc cattttgctt
    13801 ctaaagccga gtaaaggacc tacttcccgt tgcgagatca atgtctccat ttcgtcgact
    13861 tcaaactggc gtcccgccaa tattttccat aacgcaaaat cgcattgctg gcattgaaat
    13921 tttttatagg tttcatgcaa aataccgccg catttggggc acggtgtttt aagcactgca
    13981 aagtcaccac taatggtttc tccacggtga ctctttgctt gatcaaccat atgcttggtc
    14041 atctcagcga ttttattcat aaaatcttgc cgctttaatt gaccttgctc aatttgccga
    14101 agtttatatt cccaatctcc tgtcaattca ggcgagatca gttcaggaat ttttaatccg
    14161 cgtaataatg tgatcaatga aaatgctttg gccgtgggga gtaactctct tccttcacgc
    14221 agcaaataat gctcagagac taagccttca ataattgcag cgcgtgttgc tggtgtgcct
    14281 agtcctttcg agctcatcgc agctctgagt tcttcatctt caactaattt tccagcgcct
    14341 tccatggccg atagtagcgt tgattcattg aatcttgcgg gcgggcgggt ttgattggca
    14401 atggtatcga tttcttctgt tttgacagtt gcgttagggc caattgccgc taaagttaaa
    14461 ttttcttgat cgctatcagc gctagggctt gatttgccat agacgatttg ccatcctgga
    14521 ttgaccagga ttttaccttc cgtcttgaag gtttcatttt caacacgagt gattcttgtg
    14581 gtaacgagat attcagcgga gggataaaaa atcgcaagaa aacgcttggt gactaaatca
    14641 tagagcttca attccgcttc gtttagtttt ttgggctcga gtgaagtcgg aatgatcgca
    14701 aagtgatctg aaattttagc gttgttaaaa atgcgcttat tgggtttcac ccaatcagaa
    14761 acaagaattt gactggcgaa ttcgtgatag ggactcgctg ttaaagactg tagggtttct
    14821 ttgacggttt caagataatc ttccggtagc gcgcgtgaat ccgttctggg ataggttaaa
    14881 actttatgtt tctcataaag cgcttgggca agcccgaggg ttgattttgc tgaaaatcca
    14941 aagcggctat tggcttctct ttgcaggcta gttaaatcat aaagtagcgg gcaagtttct
    15001 ttggctggtt tgctttcttc gctgacagtg cctgatttac cgagacattt tagcctgatc
    15061 gcttctgcat ttgctaaatc ccagatacgt tctgcgcgtg cctcagcatt ttctttactt
    15121 ttagtaaatt tttcgtcaaa ccatttgcct ttataagaat ggctgtttgt actgaaggtg
    15181 gcatggagct cccagtaatt tttcgggatg aattttttga tggcttcttc tcgctcgact
    15241 aagattgcga gcgtaggtgt ctgaactcgg ccgaccgtcg ttttatgaaa tcccccttct
    15301 tgggaattaa aggcggtcat ggcacgagtg ccattgattc caaccaacca gtctgattca
    15361 gagcgactga ctgcagcctc ggccaagggc tgtagatcac tatcattgag tagattatta
    15421 aacccttctt tgatagcgga tggcgtcatg gattgaagcc acaagcgttt gatgggcttt
    15481 tgggtattac tatggcgcac aatatagcga aatatcagtt caccctctcg gcccgcgtca
    15541 caagcattga ttaacgtgtc gacatctttt cgcttgatga gttttgttag tgtttttaag
    15601 cgtgttgctg ttttttctaa agggattaga tcaaaatgag ggggtatgac cggtaagtga
    15661 tcaaagctcc atttgccgcg tttgacttca tattcttcag ggaccgctaa ttcgagcaag
    15721 tgtccgacag cagaggatag aatgtactca tcattctcga aataatcagc ttctttcgta
    15781 aaatttccta gaaccttggc aatatcgttt gcaacagaag gcttctcagc gataatcagc
    15841 tttttactca taaattctgt tattactttc tattagatat tttttaggtg attgggttat
    15901 gttagtactt catctgatgc tggccagatt gagtaaccaa ggttattgaa gggtgatcag
    15961 atcgatgagg ttgttttttt aagttttttc atggcattag agactcatca cgagtcaatt
    16021 ttagtgtcga ttgcgtggat tgctggcaat aaataatttt tcgattaaat cgtgattcgt
    16081 tagctggttt tgtacccata gatcgaacat gacgactaat ttgattttat ccaagcagga
    16141 actactgcca tccatctgaa gaatacgatc aattaacagc tcacgttgta atgaattgag
    16201 cacaccggct tgttcaagaa aaagaataaa cccttgactt tcaacgtcaa tgacttctaa
    16261 ctcagaagaa gaataaattc gaaacgattt actttgagat aaagttaccg ggtactttcc
    16321 tgcttcatgc ttggtatgat ccgttaacca gccaagaatc tggctaatct catcttctcc
    16381 aaatcctgcc agtgttaatt tccgtgttaa cgtctctgaa tctgggcagt tttcagaatc
    16441 aaaataattt tcgaataagt aaattaaaat ctcgaacatc gcttcgctct tcatgattat
    16501 atttcgcatt agaattttaa tgaattgtat tgttaatcaa cgaattcgtt gatatcgacc
    16561 ccctggtaaa ctagccacaa tcccatctaa ttccagagct aatagcatgg cggatactgt
    16621 ttccacagtc aagccactcc gaatacacaa tttgtcgata tcagttgctt cataatctaa
    16681 atgagaaagt aaaatcgtgt tttcttgagc gtaagtttgt ttgatcgcct catcagttgc
    16741 taacggatta gccaagttaa gcggttgata attaaactca tcgagaatgt cattaatatt
    16801 ttctacaagt tttgcacctt gcttaatcaa cgcatggcaa cctttcgata aaggtgaatg
    16861 aattgatcct ggaactgcca ggacttcacg tccttgttca agcgcttgtc gagctgtgat
    16921 gagcgagcca cttttcaaag tcgcttcgat gacgagacaa cccaagctga tgccactgat
    16981 gattcggttt ctacgaggaa aatttcttcc gattgctggc atacccaagg gaaattcaga
    17041 aatgagagcg ccttctttgg ctaattgatg ggctagctga tgattcttgg ctggataaac
    17101 gatatccaat ccggttccca caacagcaat actactggaa gaaccgagca gagcgccctg
    17161 gtgagcagct gtatcaatac ccaatgccat accgcttacg acgcaaaggc cagcactgct
    17221 tgcggcttca gcaaatgcac tcgcattgac aatgccttga ggcgtaccgt tacggcttcc
    17281 gacgattgca atggagggag tattgagtag ttcgcgctga ccttttagat agagcagtgg
    17341 aggagggtcg gcaatattga gcaatcgctt gggataattg tcatcattta atgtaatgat
    17401 tgcattagca ggatcctcaa gccacttcag tgtagcgatt attttctttt gatcaacgcg
    17461 acgattggcg atatctgcgg caattggctt ttttacatag cgctcgagaa gttgagcctc
    17521 ggcattgagg atgtccgtag gatgtttaaa agttaaaagc agcttacgga aggattcatc
    17581 accaagtcct tcaatgaagc tgagggttag ccaggattca atatctggct gaatattcat
    17641 aaccaatcaa attgttttta ttaaattgtt ttttttatgg tgtttgcacg gcatcaaata
    17701 ctttaatcgc ctgagcgcta tgcaaaacaa gagcatacga tactttatca aaaactcgaa
    17761 atagaaaaac taatccaatg cgttcatccg gtaacgagat gattttgctg tcttgcaatt
    17821 tggcggtgct tttacgataa acagcaagca catgacctac ttctaatcca tccgatgatc
    17881 ctttattaag ggcgataatc gcattttgac cgatttcagt tacaccgcca taaactgaaa
    17941 tgattcgtcc tgtgaccgga aaatcaggtg cacggggaat gtaatcgtca acatgtagtg
    18001 tagaagcagg aatcaggcgg tcgcccttga gaatttcctg caccgaatgg gtaattctaa
    18061 ctgagctgat cgcatcaaaa tttgagacct ctacatcgcc tagatatact gcctcatggc
    18121 caaggacgcg atcgttgtct tccggatcaa tgagtggttt tcctgcacgg aaaacttgcc
    18181 aagccatgcc ttgatcaaaa ggcatatttt gtacataaac agtgttgcca gtgcttaata
    18241 taacgcgatt gtcgctggaa cctaatatta atggcgcccg atccaatcca tttttttcaa
    18301 tgactaaagg ttgacttaag aacggcttga gtttttttga tggaatcgat ggaattttct
    18361 cagcctcaag tttctcaatg cgcattttgg gtgagagttt gatcgttgct ttttcattgg
    18421 caaacattag tttactgctt ccatccgcag ctcggactag aataataata tcaccagggt
    18481 agatgttatt tgggttcttg atttgttttt ggttcatgtc ccaaatttct gtccattgcc
    18541 agggatcttt aagatagcga gaggcaattc cccaaagcgt atccccttga gatacaacat
    18601 atcgttcagg cgcattggcg agtaattcga tatcttttgc ttttgcagcc cggtcagaaa
    18661 atatactgat agctaaaatc atcgctataa tggaaatagt acgcataaat ttatttttcc
    18721 aagagtgtag tggggacaaa acaaaatgga tttaaattcg attatcatcg ccgcgatttt
    18781 acgatttaac ttcggtattc agtagcgtga attacgcata tttgttatgg caattctgaa
    18841 aatattacag tacccagatg agcgattgca taagattgcg tcagttgtgc cagcaattac
    18901 tcgagatact cgtgtgttag ttgaaaatat ggcagagacg atgtatcacg catcaggaat
    18961 tggtttggct gcgacccaag ttaatgttca tcaacgtgta attgtgatcg atacttccga
    19021 aacacatgac caactcaatg tatttattaa cccagaaata ataactaagg cgggtgacgc
    19081 tgagtttcaa gaagggtgtt tatcgttacc tggcatattt gataaggtta aacgtgctga
    19141 gctggttacc gtcagggcgc taaatctcca gggggaatcc ttcgagatgc aagcagaagg
    19201 tttgctcgcg gtgtgtatac aacatgaaat cgatcatttg gaaggtaaag tttttgttga
    19261 gtacttgtcg caattaaaac aatcaagaat ccagaccaaa ttaaagaaac agcagcgcct
    19321 taccaaacct tcggtgttat cgatgagcgt ttgatgatac atcgggttcg atttttatga
    19381 ggatagtttt tgcaggaacc cctgcttttg ctgcaatcgc attaaaggca ctcatcgatg
    19441 cgcaatttca aattgtgctc gttctaacgc aacctgatcg acccgctggc cgaggattga
    19501 aactgcaaga aagtgaagtc aagaaagttg caagtgaata t
//
LOCUS       DAOQKQ010000029        18807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_1309_length_18807_cov_5.816126, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000029 DAOQKQ010000000
VERSION     DAOQKQ010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 18807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18807
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1309_length_18807_cov_5.816126"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..903
                     /locus_tag="PKZ49_03180"
     CDS             115..903
                     /locus_tag="PKZ49_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HND35594.1"
                     /translation="MILTWIILAGLAGGMLSVLFAAFLTLKTSSAWVSTLVSYAIGAL
                     LGAAFINTLPEALAIADDSRSITIIILMGILAFFILEKLVLWRHCHLDECEAHDINQH
                     TMVNIQHDHGRSGIMITLGDIFHNFVDGVLIAAAFLVDVQTGIVTSIAIIAHEIPQEA
                     GDFIILLNSGYSRLQAILLNLLASTATLVGGVLAYFMLNQLNYLIVPMLGIATASMIY
                     VAMADLIPSLHKRPAISATIQQVVLIMMGIGSIWLIEVLFSHHH"
     gene            1239..2414
                     /locus_tag="PKZ49_03185"
     CDS             1239..2414
                     /locus_tag="PKZ49_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HND35595.1"
                     /translation="MKMHAATVHQNTPSDAIFDTHPEISLDLSHVEAVLKKGYKKPFL
                     IVDRALIRNKFRRFRAVMPRVHPHYAVKANPDPRVLATLIEEGAGFEIASIAELDLLL
                     SLGVPAAEIFYSNPIKSRAYVEYAAAKGVEWYVLDSVEELRKIVSIKPDAKLYLRIDT
                     PNIGSDWPLAGKFGSHLADVKEIISEAVKLKADLAGVTFHVGSQCRNPQNWRVGIERA
                     KSVFADMEQSGLKPRLLNIGGGYPVRHTKPIPSIEVIAEVINAAIEDLSPEIHIMAEP
                     GRYLVSDSAYFVCQVVGTATRNGKRWMYWDAGIFGGIIEVSEGLRYEILTDRKGKKIP
                     WTIAGPTCDSVDILMRDELLPEDLQEGDFIYIPNAGAYTTAYASNFNGFPLPDVIVI"
     gene            complement(2487..2867)
                     /locus_tag="PKZ49_03190"
     CDS             complement(2487..2867)
                     /locus_tag="PKZ49_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35596.1"
                     /translation="MQKQYKIFVNQIIALLLALLPFGYIPQVQAQASCSGCLCPGDPC
                     RLCPLPAMTSDTVADDEPETCKKIRQEVPPIDSQPGSNEYFASLDQSTMSCVKNGGDV
                     IKNSRRNEIFTSRYYCKPDLSTRK"
     gene            complement(2959..4047)
                     /locus_tag="PKZ49_03195"
     CDS             complement(2959..4047)
                     /locus_tag="PKZ49_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HND35597.1"
                     /translation="MKLSLSSHHQLLLDALINPSTLTQYTYAQWELLLRLARKVKLLG
                     FLAVELERRKLLDTIPVRVVSQLRSSLIRAKKQQQLAFWELDRINWALKSIRIPIIVL
                     KGVAYQLAKLPTAEGRFFVDLDILVPKDDLDQVEKALLKEGWKHREISAYDEHYYRAW
                     SHEIPALIHQERETEIDIHHTISPITSKIKIDSRLLIDAAIASQSGHKILNPTDMALH
                     CVVNLFQNNDLVDNLRDLLDLHDQLQYFASHDSAFWNKLIERANQLRLGHSLYYGMQY
                     SRLIFQTAIPENTEQRLIQKPSVVSQLIMRQLVPLALFPLHPDKASKRAKFARFLLYL
                     RSHWIRMPFYLLIPHLLYKTMLRFKPGK"
     gene            complement(4037..4945)
                     /locus_tag="PKZ49_03200"
     CDS             complement(4037..4945)
                     /locus_tag="PKZ49_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HprK-related kinase A"
                     /protein_id="HND35598.1"
                     /translation="MIVEQIPVDQFIAQLSHSGIRVKLGPINVLIRTKVPQLAKQLHF
                     LYTYYDIVENEIVDIHTHVLPKFSFNNPFSSFVRFTVDGQSPFPPFPADQALAALEWG
                     INLVIAVRVNHLLMFHSAAVERNGSLILLPAWPGSGKTTLCTALIHRGYRLFSDEFGL
                     MDPISDFFTPIPRLMPLKNQSIQVIRDYLPEAVLGEEIYNTQKGTIAHVRPPRDHIFK
                     TDQVARAKWIIFPKWTANAPLKLEPLAQSEAFLLLASNSFNYEILGESAFRAVTRLIR
                     KCQCYQLVYSDLDSALAMFNKLTDEA"
     gene            complement(4962..5252)
                     /locus_tag="PKZ49_03205"
     CDS             complement(4962..5252)
                     /locus_tag="PKZ49_03205"
                     /inference="COORDINATES: protein motif:HMM:TIGR04353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr-rel-A system PqqD family peptide chaperone"
                     /protein_id="HND35599.1"
                     /translation="MEECWKTLNYGSLIIEEWDGEFTVFQPDAGKTHYLNEMSVQILI
                     YIENNASVALSTADICDYLVKKFQLQTDTAFPKQINKTLRRFDELGLVKQIS"
     gene            complement(5261..5692)
                     /locus_tag="PKZ49_03210"
     CDS             complement(5261..5692)
                     /locus_tag="PKZ49_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35600.1"
                     /translation="MSENHRNKEIEPGKDSLLDKPAQARRRLLKKSAAIPVIMTLHSG
                     AALARTSNIGTPIPDINAAATGGSSGDILCVHPDSSNGGPLGDGRYDLGTAPMVSGNY
                     QDPDNLENQALECNTRGGIIISATAYTSLSGRGVLPDSSTL"
     gene            complement(6129..9284)
                     /locus_tag="PKZ49_03215"
     CDS             complement(6129..9284)
                     /locus_tag="PKZ49_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HND35601.1"
                     /translation="MFERILRLSIDHSGLILVAVLAMALLGMNNYLRLPIDAVPDITN
                     VQVQINTTAAGYSPLEAEQRITFPIEMAMSGLPNLEYTRSLSRYGLSQVTVIFKDGTD
                     IYLVRQLVSQRLQEIRTKLPIGVIPTMGPISTGLGEIFMWTVDAEPDARKVDGTSYTP
                     TDLREIEDWIIKPRMRMVKGITEVNSVGGYVKQFHVTPYPEKLISYGLTLQDLITALE
                     RNNINVGAGYIEKSGEQYLVRVPGQVANLTEIGEIILGSNQGVPIRIKNVADVLIGKE
                     LRTGAATQNGHEVVLGTAHMLIGENSRVVSQAAAEKLAEINRSLPPGITATPVYNRTT
                     LVDKTISTVSNNLLEGAALVIVVLFIALGNLRAALITALIIPLSMLFTISGMVASNVS
                     ANLLSLGALDFGIIVDGAVIIVENCIRRLALEQEKLGRELTTAERFEIVFDASKEVRR
                     ALLFGQVIIMVVYLPVFALSGVEGKMFHPMAFTVLLALLGAVFLSITFVPAAVALFLS
                     GKMMEKEGKAVLWAKKLYAPALDSAMNNKGLTVTIAVVIITLSLLLTTRMGSEFVPNL
                     DEEDIALHALRIPGTSLSTAIEMQNELEETIKRFSEVKRVFSKIGTAEIATDPMPPSV
                     ADIFIIIKPQSEWSGKYRSKEELIAAMEKAVREVPGNNFEFTQPIQMRFNELLSGVRA
                     DVAVKVFGDDLDVMLTIAERIEKIIKNVPGAADVRIEQITGLSVLSIQMDREKMARYG
                     LNGNDVQDAINIAIGGRTAGLIFEGDRRFELQVRLPESIRGDIESLKRLPIKLPSVLI
                     PAVGQNVQPALTPNYVTLAEIASLEIVKGPNQISRENGKRRVVITANVRGSDIGSFVT
                     EAEQQIINSVKLPSGYWLAWGGQFEQLILAAERLQIVIPLALGLVFFLLYMMLNSLRD
                     SLLVFSAVPLAITGGIVALWLRDIPLSISAGVGFIAMSGVAVLDGLVLISFIRDLRQQ
                     GLPLNDAIRTGSLLRLRPVLITTWVESLGFLPMAISTSTGAEVQRTLATVMIGSTVSQ
                     SLLSLLVLPVLYQLTHRNNRS"
     gene            complement(9323..10822)
                     /locus_tag="PKZ49_03220"
     CDS             complement(9323..10822)
                     /locus_tag="PKZ49_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HND35602.1"
                     /translation="MNKARLIPILIVIIVGIVLGALILTVDKSPGNSPEQAADNKTMQ
                     LEPNQSNTGPRGGSYFSDNEFGLELTIFEQGVPPQFRIYLYEKGKALPPIAAKVSIAL
                     SRLGAPIQLIYFSPEADYLLGDQVINEPHSFELVITAEWNGKTMRWKMSQVEARLEMT
                     NEMLKTIGVEIQTAGPATIQSTLRLPGEIVLNPDRMVQVVPRLPGVVIAVNFEIGQTV
                     KKGQVLAVVESQILADLHSQYIAAQKRLALAQTVYEREKLLWEEKISAKQDYLAAQLA
                     LNEAKIASDLSSEKLRALGVQPKVGLQAKDLTRYEILAPISGLIVTRTIALGAALKDD
                     VTIFTLADMASVWATITVYPKDLSLIKIGQKASIKATAFDVEGSGIVSYVSPLIGGQT
                     RTATARVILDNQDGRWHPGMFVNIELVSEEIEVPIAVPVQALQALRDWTVVFGRYGDY
                     LEARPLELGRSDGKMVEIIKGLSAGEQYATGNSFAIKADMEKAGTSHDH"
     gene            complement(10850..12160)
                     /locus_tag="PKZ49_03225"
     CDS             complement(10850..12160)
                     /locus_tag="PKZ49_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HND35603.1"
                     /translation="MNDLIKTTVTEQNKLNADDGKNITLINAINLTLLHNPELAALAK
                     EIGALGGATLQAGLFRNPELSFNVENPGNIQKLRGDINSPDSVAQEVVQQLTTIRIGQ
                     LLELGGKRMARVQAAQLNEELAVRDYESRRIEIIARVANVFTEVLAGQERLKLSEETK
                     LLAQNVVDKVVLRVQAGKVPPIEETRAKIGLSTARIEWEQAQRDLASARKRLALMWGS
                     TLPQFDQALGSLETSTPPPDFHRLQELVMSSPMALRAMKNIEHRKALVEVEETRRIPN
                     LTVSAGIVHHAQLGGTTAVAGLMIPLPLFDRNQGNLKEAHVRVNKAEDERQALELRLK
                     TELTQSYESLTAAWNEINILRNEILPGAKSAFNVMRRGYELGKFGLLELLDSQRVLFQ
                     NQLLYVRALANYQRLINDIERLIAAPIDTISPSQTNDTQSGKNY"
     gene            complement(12478..12813)
                     /locus_tag="PKZ49_03230"
     CDS             complement(12478..12813)
                     /locus_tag="PKZ49_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35604.1"
                     /translation="MLTNTLGWSFNSKVYTHELEHLRYSELFTANPENHRELHRQLSD
                     NGEIDAATHLCLHAAGQYQPFYFNPIITAIMVLNSIEAILVNNPKLLPEIILEVFLRP
                     PISSNTSFF"
     gene            complement(12986..13585)
                     /locus_tag="PKZ49_03235"
     CDS             complement(12986..13585)
                     /locus_tag="PKZ49_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HND35605.1"
                     /translation="MNRSIITASKIILGSSSQYRRELLSRLKLPFEVVSPEVDESALP
                     FETPEVTAMRLTKKKASVIAQSYPDALIIAADQVAAMGNIHLGKPLSHAKAKIQLSMM
                     QGKEVNFYTALCLLNSRTNHLQEHITRYIVKFRPLSDQQIENYLTKEQPYHCAGSAKA
                     EGLGIALIEKMTGNDPNALIGLPLIALVDMLLNENVEVL"
     gene            13657..14154
                     /locus_tag="PKZ49_03240"
     CDS             13657..14154
                     /locus_tag="PKZ49_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HND35606.1"
                     /translation="MENIFIIDPLSFAQNKRIRHGKIAGDKLDRLRELIDSDEGFAYV
                     IEGDTDKLARPTLRLQIKGKIAVQCQRCLDKLNHEVDIDTLLILARNARELELYDADN
                     TIDAVLALHELDMGALIEDEIILSLPISPRHQEGGCNKDMQSILKREHKESPFSSLVS
                     LKQTH"
     gene            14181..14363
                     /gene="rpmF"
                     /locus_tag="PKZ49_03245"
     CDS             14181..14363
                     /gene="rpmF"
                     /locus_tag="PKZ49_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HND35607.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDFLTNPPLAIEPTTGETHLRHHISP
                     SGYYRGRKVIKTKNDE"
     gene            14428..15453
                     /gene="plsX"
                     /locus_tag="PKZ49_03250"
     CDS             14428..15453
                     /gene="plsX"
                     /locus_tag="PKZ49_03250"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HND35608.1"
                     /translation="MNITVAIDCMGGDHGPHVTVPSAAEYLLADPETDIILVGIPDAI
                     EAELKAIGVSLSTRLRIHAASEVVSMDEVPAFALRYKKNSSMRVAIDLVKNGEALACI
                     SAGNTGALLATSRFVLKTIPGIDRPALAVVLPTIKGHTYILDLGANVNCTAEHLLQFG
                     VMGATLVSSVENKKMPSIGLLNIGEEDIKGNEVVKQAAELLRTSGLNFYGNIEGDDIY
                     KGTTDVVVCDGFVGNVALKTSEGVAQMLATFLRQEFKRNIFTKLVGIVASPVINAFKQ
                     RVDHRRYNGASLLGLRGIVIKSHGSADKYAFTAAIRRATDEVRGGMLKHISERVAELQ
                     QGIGKNN"
     gene            15485..16441
                     /locus_tag="PKZ49_03255"
     CDS             15485..16441
                     /locus_tag="PKZ49_03255"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HND35609.1"
                     /translation="MYSKIAGTGSYLPEKILTNIELESIVDTSDEWIQTRTGIAQRHI
                     AADDQMASDLALAASRRALDAANLKAQDIDLIILATTTPDMIFPSTACILQEKLGITD
                     GPAFDVQAVCSGFIYALATADMFVSSGKSKNALVVGTEIYSRIIDWKDRSTCVLFGDG
                     AGAVVLSQSEQPGILSSHLHANGGYSKILAAPGSISGGKIQGTPFIAMEGNTVFKFAV
                     KALEEAAIESLAANNLQASDIDWFIPHQANIRIIKSTAHKLGIPMEKVVTTVDRHGNT
                     SAASVPLALDLAVRDGRIQPDHYVLLEGVGGGFTWGSILLQW"
     gene            16446..17396
                     /gene="fabD"
                     /locus_tag="PKZ49_03260"
     CDS             16446..17396
                     /gene="fabD"
                     /locus_tag="PKZ49_03260"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HND35610.1"
                     /translation="MIIAFTFPGQGSQSVGMLNGYSDLPIIKQTFDEGSEILGYDCFE
                     LAKHGPVEQLNLTIHTQPIMLMAGIAVYRAWKSLGGGSPDYLAGHSLGEYTALVAAEA
                     LAFRDAIKLVRYRAEVMQECVPNGMGAMAAIIGLEDDAVKAICNQIVGDNEKVVLEPA
                     NFNSPGQIVIAGHKAAVLEGMALAKSKGAKLVTLLPLSIPSHCSLMYPAAEKFQKLLE
                     QIDFYSPKIPVIHNADVQQHQQSNEIKKILMQQIHSPVHWVNTIQSFVSQGVTHIVEC
                     GPGKVLSGLTKRIDKTPTNYSLTDSESLQQAIEKLNQPAF"
     gene            17406..18158
                     /gene="fabG"
                     /locus_tag="PKZ49_03265"
     CDS             17406..18158
                     /gene="fabG"
                     /locus_tag="PKZ49_03265"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HND35611.1"
                     /translation="MEKKLLEGNIAFVTGASRGIGMAITLELAKQGAIVIGTATTQNG
                     ADGITRQLEEISAPGMGVVMNVNDVDQIGKAFEMTNKQFGDISILVNNAGITRDNLLV
                     RMKDEEWDEVMTTNLKSIFYLSKIALRAMMKARSGRIINISSVVGATGNPGQANYAAS
                     KAGMIGFTKSLAREVGSRNVTINCVAPGFIDTDMTSSLTTEQRLNLLKQIPLGRLGQS
                     ADIAAAVVFLASPAASYITGTTLHVNGGMFME"
     gene            18262..18507
                     /gene="acpP"
                     /locus_tag="PKZ49_03270"
     CDS             18262..18507
                     /gene="acpP"
                     /locus_tag="PKZ49_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HND35612.1"
                     /translation="MDNTDVEQRIRKIVAEQLGVNESDVKNESSFVNDLGADSLDTVE
                     LVMALEEEFDCEIPDEEAEKINTVQEAIDYVNSHSSN"
     gene            18543..>18807
                     /locus_tag="PKZ49_03275"
     CDS             18543..>18807
                     /locus_tag="PKZ49_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HND35613.1"
                     /translation="MSKRRVVVTGLGIISPVGNNVSDAWQNIVSGNSGITRINRFDAS
                     NFSSQIAGEVKNFDITHYLSAKDARRMDIFIHYGMAAGIQAIRD"
BASE COUNT         5397 a         3628 c         4122 g         5660 t
ORIGIN      
        1 aataagctat attaagattt aagtaattct gatcgtgaat gtatagatga atccagcact
       61 cggatgttta tgtgctggat tttgtttttt tgatagatta ttctgaataa ttttatgatt
      121 ttaacttgga tcatcttggc tggtttagcg ggaggaatgt taagcgtttt atttgcggcc
      181 ttcttgacat taaaaacgag tagtgcctgg gtatcaacac tcgtttctta tgcaatcggt
      241 gcattacttg gcgcagcttt tattaataca ctccctgagg cattagcaat tgcagatgat
      301 tcacgatcca ttactatcat catattaatg ggaatattgg cattctttat tttagaaaaa
      361 ctggtgttat ggcggcattg ccatttagat gagtgtgagg cgcatgatat taatcagcac
      421 acgatggtga atattcaaca tgatcatggt cgtagtggaa tcatgataac cctcggcgat
      481 atttttcata actttgtcga tggtgttctg attgctgctg cttttttagt tgatgttcaa
      541 acgggtattg ttacctcgat agccattatt gcgcatgaga ttccgcagga agcgggagat
      601 tttattattt tactcaattc gggctatagt cggctgcagg ccatattatt gaatctgctg
      661 gctagtactg ctacattagt aggaggggtg ctcgcttatt ttatgctcaa tcaacttaat
      721 tatttgattg taccgatgct tggcattgca actgcaagta tgatctatgt agctatggct
      781 gacttgatcc cgagcttaca taaacgccca gcaatttctg caactataca gcaagtggtt
      841 ttgattatga tgggtattgg gtctatttgg cttatcgagg ttttatttag ccatcatcac
      901 taagatgggg ataccatcag agactacatt aacggtttcc tgattgaata ccctaaaaat
      961 tcttcccctc tcttgcaatg tagtaatcca ctgcgtgagc gtttataacg gagcgagtcg
     1021 cgaagttatt cttgctttaa aaatgccgct atttctttag ttaaaaagag cttgattctt
     1081 gaaatttgaa gactataatt cgtcccttta acatatccat tattgtagtg tttgtaagga
     1141 ggtcatcatg aaaagcaaag taattttttc tattccaaca gacctagttt ttattcatat
     1201 tcctgaaacg tagtgacttt tggaaggaaa tcaaaatcat gaaaatgcat gctgccactg
     1261 ttcatcaaaa taccccatct gatgcgattt ttgacacgca tccagaaatc agcctagatt
     1321 tatcccacgt tgaagcggtc ctaaaaaaag gatataaaaa acctttttta attgttgatc
     1381 gcgctttgat tcgcaataaa ttccgacgat ttagagcggt aatgccgcgt gtgcatcctc
     1441 attatgcggt caaggcaaac cctgatcccc gtgttctagc aaccttaata gaagaggggg
     1501 ctggttttga aattgcatca attgctgagc ttgatttatt gcttagctta ggcgtgcctg
     1561 ctgcagaaat cttttatagt aatccgatta aatctagagc ctatgtcgag tacgccgcag
     1621 ctaaaggtgt tgaatggtac gtgctggata gcgtcgaaga attacgtaaa attgttagta
     1681 ttaaacctga tgcaaagctt tatttgagaa tcgatacacc caatatcggt agtgattggc
     1741 ccctggccgg aaaatttggt tctcatttag cggatgtaaa agaaattatc agtgaggctg
     1801 tgaaattaaa agcagatctg gcaggtgtaa cttttcatgt aggctcacaa tgtcgtaatc
     1861 ctcaaaattg gcgagtagga attgaacgcg ctaaaagtgt atttgctgac atggagcaat
     1921 ctggacttaa acctcggcta cttaatattg gtggggggta tccagttaga catactaaac
     1981 ccatcccttc gattgaggtt attgctgaag ttattaatgc tgccattgaa gatctttcac
     2041 ctgaaattca tatcatggca gaacctggca gatatttagt gtctgattct gcctattttg
     2101 tttgtcaagt tgtgggtact gcaacgcgta atggtaagcg ctggatgtat tgggatgcgg
     2161 gaatatttgg cggtatcatc gaagtcagtg agggattgcg ttatgaaata ttgacagacc
     2221 gcaaaggaaa gaagattccc tggactattg ctggaccaac ttgtgattcg gttgatatac
     2281 tcatgcgtga tgagttatta ccggaagatc tccaagaagg tgattttatt tatataccaa
     2341 atgctggcgc ttatactacg gcttatgcca gcaactttaa tggttttcca ttaccggatg
     2401 taattgtaat ttaagtgata cgcgttatac ctactcgttc tagtagattt aatcaatcac
     2461 tagaacgggt tagtgggttt ggactactat tttctagtgc tcaaatcggg tttacagtaa
     2521 tagcgtgaag taaaaatctc gttccggcgc gagtttttga ttacatctcc cccatttttt
     2581 acacacgaca tcgttgactg atcgaggcta gcaaaatatt cattggagcc tggttgtgag
     2641 tcaatgggtg gtacttcttg ccgaattttc ttgcaagttt caggttcatc gtcagcaaca
     2701 gtatcgctag tcatagcggg taaagggcat aaacggcatg ggtctccagg gcaaaggcat
     2761 ccactgcatg aagcctgggc ctgaacttga ggaatatagc caaacggaag tagtgctaat
     2821 aatagcgcga tgatctgatt cacaaatatt ttgtattgtt tctgcattta atacctcgtt
     2881 ttatctgaat ttaatactca atttaccagc tattccactt agagagtaaa attatacgtg
     2941 aagtgattgt tgttcacttt atttccctgg tttgaatcgc aacatcgttt tgtataacag
     3001 atggggtatg agtaagtaga aaggcattct gatccaatgg ctccgcaggt agagcaagaa
     3061 acgtgcaaat tttgctcgct tagaagcctt atcaggatgg agtggaaata aggccaatgg
     3121 gaccagttgt cgcatgataa gctgggaaac tactgaaggt ttctgaatta ggcgttgctc
     3181 agtattttca gggattgctg tttgaaatat caatcgagaa tattgcatgc catagtaaag
     3241 tgaatggcct aaccggagct gattggcccg ttctatcaat ttattccaaa acgctgagtc
     3301 atggcttgcg aagtattgca attggtcatg caaatcaagt aaatcgcgta agttatctac
     3361 taaatcgtta ttttgaaata gattaacaac gcaatgtaga gccatatcgg tagggttaag
     3421 aattttgtgg ccggattggc ttgcaatggc tgcatcaata agcaaacgtg aatcaatttt
     3481 gattttgctg gtaatgggcg agatcgtatg atgaatatcg atttcagttt ctctttcttg
     3541 gtgaatcagc gcgggaatct catggctcca agctcgataa taatgttcgt cataagctga
     3601 aatttcgcga tgcttccatc cttcttttaa taatgctttc tcaacttgat ccagatcatc
     3661 tttgggaacc aaaatatcga gatcgacaaa aaaacgcccc tcagcagtag gcaatttagc
     3721 aagttgataa gcaacacctt tcaatacgat aatggggatc cgaatagatt taagagccca
     3781 atttattcta tccaactccc agaatgcaag ctgttgttgc ttctttgcgc gaattaaact
     3841 ggaacgtaat tgactcacca cccttaccgg gattgtatct agcagctttc ttctttccaa
     3901 ttccaccgcg agaaagccta atagtttgac tttccttgct aaccgtaata gtaattccca
     3961 ttgcgcatag gtatactgag tcaatgtact tggattaatg agcgcatcca gtaacagttg
     4021 atggtgacta gataaactaa gcttcatcag taagtttatt gaacatagct aatgcgctat
     4081 caagatcaga gtaaacaagc tgataacatt gacactttct aataaggcga gttactgctc
     4141 tgaacgctga ttcgcctaaa atttcatagt tgaaagaatt gctagcaagt aataaaaatg
     4201 cttcagattg tgcgagaggt tccagtttta aaggcgcatt tgcagtccat tttgggaaga
     4261 tgatccactt cgccctagca acctgatctg ttttaaagat atgatcacga gggggacgaa
     4321 catgggcaat ggtgcctttc tgagtattgt aaatttcctc acctagtacc gcttctggta
     4381 gataatctct aatgacctga atagattgat tcttgagtgg cattaatcga ggtatgggtg
     4441 tgaaaaaatc tgaaatggga tccattaaac caaactcatc cgagaacaac cgatatcctc
     4501 tgtgaataag agcagtgcag agcgtagttt tgccacttcc tggccaagca ggtagtagta
     4561 ttagcgaccc atttctttcc accgcagcag aatgaaacat taacaagtga ttgactctta
     4621 ccgcaataac caaattaatc ccccattcta acgcagctaa tgcctgatca gccgggaaag
     4681 gtggaaaggg cgattggcca tctacggtaa agcgaacgaa tgatgagaaa ggattgttaa
     4741 atgaaaattt aggcagaaca tgcgtgtgaa tatcaacgat ttcattttcg acaatatcgt
     4801 aataggtata aagaaaatga agctgttttg ctagctgggg aaccttggtt cgaattagaa
     4861 cattgatagg acccagttta actcggattc ctgaatgaga taactgagct atgaactgat
     4921 caaccggaat ctgctcaacg atcacacgaa ttgttttatc tttaagaaat ctgctttact
     4981 aaacctaatt catcaaagcg acgcaaggtt ttattgattt gcttaggaaa agcagtatca
     5041 gtttgtagtt gaaatttttt gactaaataa tcacatatat ccgctgttga taaagcaacg
     5101 gaagcattgt tctcaatata tattaatatc tgaacactca tttcattcaa ataatgagtt
     5161 tttcccgcat ctggctgaaa aacagtaaat tcaccatccc attcttcaat aattaaagaa
     5221 ccatagttta gtgttttcca acattcttcc acgaatgagc ttatagtgtg cttgagtccg
     5281 gtaaaacccc tctaccactt aaggaagtat aagccgtagc tgaaataatg ataccacctc
     5341 ttgtattaca ctctaaggcc tgattttcta aattatcagg atcttgataa tttccactga
     5401 ccattggtgc agttcctaaa tcatagcggc catcacccag tgggccgcca ttgctgctat
     5461 caggatgtac gcataggata tcgccgctac taccacccgt agcagctgca ttgatatcag
     5521 gaataggggt ccctatatta ctggttctcg caagtgctgc accgctatgc agtgtcatta
     5581 tgactggtat tgcagccgat tttttcagta atcgtcttct tgcttgtgct ggcttatcca
     5641 gcaaagaatc ctttcctggt tctatttctt tgtttctatg attttcagac atatttacga
     5701 aactcctaat ggctatcgag gaattcgatt tacacgcgca ttaaagtatt taaatgctaa
     5761 ctatttacaa tctctatcga tagccattat agcacttact taaagatgat cgagtatttc
     5821 aaattaccta cagattatca gtatcttaga tcacttattc tctaagagtt aaatcttttc
     5881 aaagtgggat actcattaat atatattaat atttatttgt ttctaggttt aatcaagtcg
     5941 aatcttcata acttaaggta ataacctaaa agctttattt tttaatacgc aagatacatg
     6001 ccatccagta gaatattttt gtaaatcaaa tagaaacaag tggatggaaa gatatttttt
     6061 tcctttaggt tagttaatag aaaaatatgt aaagaaattc gacagactat atcacctggt
     6121 tactaaaatt aactgcgatt attacgatgt gtaagttgat aaagcactgg tagcacgaga
     6181 agggaaagaa gtgactgaga gacggtactt ccaatcatta cagttgcaag tgttctctgt
     6241 acttctgcgc ccgtgcttgt gctaattgcc atcggcaaga aaccaagtga ttctacccaa
     6301 gttgttataa gaacggggcg gagtcttagt agagagccgg ttcgaatagc atcattaagt
     6361 ggaagtcctt gttgtctgag atcacggata aatgatatca gcactaatcc atctaataca
     6421 gcaacgcctg acatggcaat aaatcctaca ccggccgaaa tcgataatgg aatatcgcgc
     6481 aaccataagg caacaatacc accggtaatg gcgagcggca ccgcgctgaa aacgagcaaa
     6541 ctgtccctga ggctattaag catcatatat aagagaaaaa atacaaggcc gagcgccagt
     6601 ggaataacaa tttgcaaacg ctctgcagct aagattagct gctcgaattg tcctccccaa
     6661 gctaaccaat agcctgaagg aagcttaacg ctattgataa tttgttgctc agcttctgtt
     6721 acaaagctac caatatcgct tccacgaaca tttgcagtaa taacgacccg gcgttttccg
     6781 ttttcgcgac tgatttgatt tggcccctta acgatttcta ggctggctat ttccgctaat
     6841 gttacgtaat tgggtgtaag cgcaggttga acattctggc caacggctgg gattaaaact
     6901 gatggtaact tgatcggtaa acgtttcagt gattcaatat caccgcgaat cgactcgggt
     6961 agtcgaactt gtaactcgaa gcgacgatct ccttcgaaaa taagccctgc agttctacca
     7021 ccaatcgcaa tattgattgc atcttgaacg tcatttccat tgagaccata gcgagccatt
     7081 ttttctcgat ccatttgaat cgaaagcacc gataaaccgg taatttgttc aattcgtaca
     7141 tctgcagcac cggggacatt tttaatgatt ttctcaatcc gctcggcgat ggttaacatg
     7201 acatcaaggt catcaccaaa tacttttacc gctacatcag cacgcacgcc agacaataat
     7261 tcgttgaagc gcatttgtat cggttgcgta aactcgaaat tattaccggg tacttctcgt
     7321 acggcttttt ccatagcagc aatgagttct tctttgctgc gatatttgcc agaccattca
     7381 gattggggtt tgatgatgat aaaaatatca gcaacgctgg gcggcatcgg atcggtagcg
     7441 atttcagccg tcccaatttt agaaaatacg cgctttactt ccgagaatcg cttgatggtt
     7501 tcctctaact cgttttgcat ttcgattgct gtactaagac tagttccagg aatacgaaga
     7561 gcgtgcagag cgatatcttc ttcatcaagg ttgggaacaa actcgctacc catacgagtg
     7621 gtcaataata gagacaatgt gatgataacc acagcgatgg ttacggtgag tcctttgttg
     7681 ttcatcgcac tatcgagggc tggtgcgtag agtttcttag cccaaagtac ggccttacct
     7741 tctttctcca tcattttgcc agaaagaaat agcgctactg cagcaggtac aaatgtgatt
     7801 gacaaaaaaa cagccccgag taacgccaat aaaacggtga aggccattgg atgaaacatc
     7861 tttccttcca cacccgaaag tgcaaaaaca ggtagataga ccaccataat aattacctgc
     7921 ccaaagagca aagcacgtcg cacttctttt gaagcatcaa aaacgatctc aaagcgttcg
     7981 gcagtagtta gctcacgccc taatttttct tgttctagcg ctaggcgtcg aatacagttt
     8041 tctacaatga tgactgcacc atcaacaata ataccgaagt caagcgcacc caagctgagt
     8101 agattggcac tgacattgct ggctaccata ccgctgatcg taaatagcat ggaaagtggg
     8161 atgattagag cagtgatgag tgcagcacgt agattgccca gtgcgataaa caaaaccacg
     8221 attacaagcg cagcaccttc tagtagatta ttggaaacgg tgctaatggt tttatccacc
     8281 aaagttgtgc gattgtaaac aggcgtggca gtaatacccg gaggcaaact gcgattgatt
     8341 tcagcaagct tttcggccgc agcttgcgaa accacacggc tattttctcc aatgagcatg
     8401 tgggctgttc ccagcacgac ttcgtggccg ttttgtgtgg ctgcacccgt tcgcaattct
     8461 ttgccgatta acacatccgc tacattcttg atacggattg ggacaccttg attgctgccg
     8521 agaatgattt cgccaatttc agtcaaatta gccacttggc cgggtactct aactaagtat
     8581 tgttccccac ttttttcgat gtagcccgcg cctacattaa tattgtttcg ttccagtgcg
     8641 gtaatgagat cctgtaaggt aagcccataa gaaatcagtt tctcagggta gggcgttaca
     8701 tggaattgtt tcacgtaacc accaactgaa ttgacttcgg tgataccttt caccattcgc
     8761 atacgtggtt tgataatcca gtcttcaatt tctcgtagat cggttggggt atatgatgtc
     8821 ccgtcgactt tgcgtgcatc gggttccgca tcaaccgtcc acatgaaaat ttcacctagc
     8881 cctgtggaaa tggggcccat ggtcggaata acgccgattg gtaacttcgt tctaatttct
     8941 tgtaagcgtt gactaacaag ttgccggact aggtagatat ccgttccgtc tttaaaaatg
     9001 accgtcactt gcgataagcc ataacgcgat agagaacgag tatattcaag gttaggcagc
     9061 ccagacatag ccatctcgat cggaaaggta atgcgctgtt cagcttctag tggagaatag
     9121 ccagcggctg tcgtattgat ttgcacttgt acattggtaa tatcgggcac ggcatcaatc
     9181 gggagcctga ggtagttatt catgcccaat agcgccatgg ctaatacagc aacaaggatc
     9241 aatccactat gatcaatgga taggcgtaga atgcgctcaa acatattttt tccttgtgag
     9301 ttttctatcc agaaaaattg gcttaatgat catggctggt tcctgctttt tccatatcag
     9361 ccttaatggc aaaactattg cctgttgcat attgctcacc ggcagacaag cctttgatga
     9421 tttctaccat tttgccatca ctgcgtccga gctctagggg ccttgcttct aaatagtccc
     9481 catagcgacc aaaaaccact gtccaatcgc gcaaggcttg taaggcttgt accggaacgg
     9541 caataggcac ttcaatttct tcagaaacaa gttcgatatt gacgaacatt cctggatgcc
     9601 atcggccatc ttgattatct aaaatgacac gggctgtggc ggtacgagtt tgccctccaa
     9661 tgagggggga aacataagag acgataccgc tgccttctac atcaaatgca gtcgctttga
     9721 tcgaagcttt ctgaccgatc tttatgagac ttaagtcttt tggataaacc gtaattgttg
     9781 cccaaactga agccatatca gcaagtgtga aaatagttac atcgtcttta agcgcagcac
     9841 cgagtgcaat cgttctcgtt acaataaggc cagaaatagg tgcaagaatt tcatatcgag
     9901 ttagatcttt tgcttgtaag ccaactttag gctgcacacc caatgcacgt agtttttctg
     9961 aggataaatc agaagcaatt ttggcttcat tcagtgcaag ttgtgctgct agataatctt
    10021 gcttggctga gattttctct tcccaaagca atttctccct ttcatataca gtttgagcca
    10081 gtgcaagtcg tttctgagca gcaatatatt ggctatgtaa atctgctaga atttgacttt
    10141 ctacgactgc gagtacttgc cctttcttta cagtctgtcc aatttcaaag tttactgcaa
    10201 taacaacacc aggtaggcgt ggtacgacct gtaccatccg atctgggtta agaacaatct
    10261 caccgggtaa cctaagggta gattgaatcg tcgctggtcc cgcagtttga atttcaaccc
    10321 cgattgtttt taacatttca ttagtcattt ccaagcgagc ttcaacctga ctcattttcc
    10381 accgcattgt tttaccgttc cattctgcag taatgactag ttcaaatgag tggggttcgt
    10441 tgatgacttg atcacctaat aagtaatctg cttcaggtga aaagtaaatg agttgtatcg
    10501 gtgcaccgag gcgtgataat gcgatagata cttttgcagc aatgggaggc agagctttac
    10561 ctttttcata aagataaatt cggaattgag gcggaacgcc ctgctcaaaa atagtgagtt
    10621 ccaaaccaaa ttcattgtcg gaaaagtagc tgccgccccg tggtccggta ttggactgat
    10681 tgggttcaag ttgcatggtt ttattatcag cagcttgctc gggtgagttt cctggggatt
    10741 tatcgactgt taggatcaat gctccgagga ctatgcccac gataataacg attaagattg
    10801 gaatcaatcg cgctttattc ataatttttc cttttaattc ggtagcgctt taatagtttt
    10861 tgccagactg agtatcattt gtttgggaag gcgagatagt gtcgattggt gctgcgatta
    10921 gacgttcgat atcattgatg agacgctgat aattggctag tgcacgaaca taaaggagct
    10981 gattctgaaa gaggacccgt tgtgagtcca atagctccaa taagccaaat ttaccaagct
    11041 catagcctct gcgcattacg ttaaaggcgc ttttggcgcc aggtagtatt tcattccgca
    11101 aaatatttat ttcattccaa gcagctgtca aagattcata ggactgtgtg agctcggttt
    11161 ttagtcgaag ctctagcgct tggcgctcat cttccgcttt attaacacgg acatgtgctt
    11221 cttttaaatt accttgatta cgatcgaata agggtaaagg aatcatgagt cccgcaaccg
    11281 cagtggttcc acccagttgt gcgtgatgga cgataccggc actgacggtg agattgggta
    11341 tgcggcgcgt ttcttctact tctacgagtg ctttccggtg ttcaatgttc ttcatggcac
    11401 ggagcgccat tggactggac atgacaagct cttgcaatcg atgaaagtca ggaggaggcg
    11461 tggatgtctc tagagatcct agcgcttgat caaattgtgg caacgtacta ccccacatta
    11521 atgcaagcct tttgcgtgca gaagctaaat cgcgttgtgc ttgttcccat tcaatgcgag
    11581 cagtcgagag acctatctta gctcttgttt cttcaatggg agggactttt ccagcctgta
    11641 ctcttaatac aactttatcg actacatttt gtgcaagtaa tttagtttcc tctgacagtt
    11701 tgagacgttc ttgacctgct aatacttcgg tgaacacgtt agcgaccctt gcaataatct
    11761 ctattcgcct ggattcataa tctcttactg ctaactcttc gttcaattga gcagcttgaa
    11821 ctctggccat tcgtttgccg cctaattcaa gtagttgccc tatcctgatt gtagtgagct
    11881 gctgaacgac ttcttgagca actgaatccg gggagttaat gtcgcctcgt agtttctgga
    11941 tgtttcccgg attttcaaca ttaaatgaca attctggatt tctgaataac cccgcttgta
    12001 atgttgctcc ccctaaagcg ccgatttcct tggctagcgc agcgagttcc ggattatgta
    12061 gtaaagttaa attgattgcg ttgattaatg taatattttt accatcatcc gcatttaact
    12121 tattttgctc agtcactgtg gtttttatca ggtcattcat gaatagctca gaattggctt
    12181 ttgctggttg tacgcaactg attactgaga ataacagcaa ccaaataatt tgcttaggta
    12241 gcaaccaatt taaccttagc cgattgagat gtatacaacc catgctatcc ctctaatatg
    12301 aaaatactta acttgatgcg gatccaacag atctaattca gttgcgttga aacctaaccg
    12361 gtaatactac taatttttac taacagcgaa ggaaagaatc aatattccta ttgatgacgc
    12421 aataccacaa agctgtattt tgtagaaact ttaactagat caaccagttt aactaaacta
    12481 gaaaaatgat gtatttgatg agatgggggg gcggagaaaa acttcaagga ttatctctgg
    12541 aagcagttta gggttattta ctagtatggc ttcaattgaa ttaagaacca tgatagccgt
    12601 gataattgga ttgaaataga agggttggta ttgtccggcg gcatggagac aaagatgggt
    12661 ggccgcatcg atttcaccat tatcagaaag ttgacgatgc aattcgcggt gattctcagg
    12721 attggctgta aacagctcgc tatatcgaag atgctctagc tcatgggtgt ataccttgct
    12781 attaaatgac caacctaacg tattcgttag catggttata atgaaaagca agcagaccgt
    12841 ctttattaga taagaatggt aaggtataag aaacataacc tattttaatc gatctagttt
    12901 ttttagcgat gcttattata gtatagcgtt tgagtaatct agatcatttt ttttaagcgg
    12961 tacttcttta accctatagc aaaaatcaga gtacttcgac gttttcattc agtaacatgt
    13021 caacgagtgc aatcaacggt aggccaatta atgcattagg gtcgttccca gtcatttttt
    13081 caatcaatgc aatgccaagc ccttctgctt tagcgctgcc agcacaatga tagggctgct
    13141 cctttgttaa gtaattttca atctgctgat cacttagggg gcggaattta acaatatagc
    13201 gtgtgatatg ttcttgtaaa tggttagttc gactatttag aaggcaaagt gcggtataga
    13261 aattcacttc ttttccttgc atcatgctaa gttgaatttt tgctttagca tgacttaacg
    13321 gttttcctag gtgaatattt cccattgcag caacttgatc agcagcaata atgagcgcat
    13381 cggggtagga ttgggcaatc acgcttgctt tctttttagt caatcgcatt gcggttactt
    13441 ctggcgtttc aaatggcaat gcgctttcat cgacttccgg gctaactacc tcaaatggta
    13501 gttttaatcg cgataagagc tctcgacgat actgagagct agagcctaaa ataattttcg
    13561 aagcagtgat aattgaacga ttcacagaaa attcttatta taaatagatg cgttgttttt
    13621 gacactgatt tctaaaaaat atagcattgc aatgttatgg aaaatatttt tattattgac
    13681 cctttaagtt ttgctcagaa taagagaatt cggcatggta aaattgcagg ggataagtta
    13741 gatcgtctac gtgaattgat cgattctgat gaagggttcg cttacgttat tgagggcgat
    13801 acggataaac ttgctagacc tactttgagg ttgcaaataa aaggaaaaat tgctgttcaa
    13861 tgccagcgct gccttgataa gctaaatcat gaggttgata tcgatacgct attgattttg
    13921 gcgagaaatg cacgtgagct tgagttatat gatgcagaca atacgattga tgcggtgtta
    13981 gccttgcatg agttagacat gggtgcatta atagaagacg aaattatcct aagtctacct
    14041 atttctccac ggcatcagga gggggggtgt aataaagata tgcaatcgat cttgaaacgt
    14101 gagcataaag aaagtccgtt ttcttcgctt gtatcactga aacaaacaca ttaatcaaat
    14161 taattgggag tagtaatttt atggctgttc aacagaataa gaaatcacca tcgaagcgtg
    14221 gtatgcatcg ttcacacgac tttttgacaa atccaccact tgctattgag ccgacaacag
    14281 gtgaaacgca tttgcgtcat catatcagtc cttctggtta ctatcgtggt agaaaggtaa
    14341 ttaaaaccaa aaacgatgaa taaagctttt tagagactta tttttcatta tctttttatt
    14401 gaatagtatt ttgataatca acgcaaagtg aacattactg tagcgattga ttgcatggga
    14461 ggcgatcatg gtccccatgt tacagtaccc tctgcagcag aatatttatt agctgatcca
    14521 gaaacagaca ttatattggt cggtatacct gatgcgatcg aagctgagtt aaaagccatt
    14581 ggtgtatcgt taagtactcg tttaagaatc catgcggcaa gtgaagtagt tagtatggat
    14641 gaagtgccgg cattcgcttt acgctacaaa aaaaattcgt cgatgcgagt ggcgatcgat
    14701 ttggtcaaaa atggtgaagc tttagcttgt attagtgctg gtaacacggg tgcgttatta
    14761 gcaacctcgc gttttgtatt aaaaacaatt ccaggtattg atcggccagc gctagcagtc
    14821 gttttaccga cgataaaggg gcatacttat attttagatt taggggctaa tgtcaattgt
    14881 accgctgagc atttgctaca atttggcgta atgggtgcga cgctagtatc ttctgttgag
    14941 aataaaaaga tgcccagtat cggtttgctt aatatcggtg aagaagatat taaaggtaat
    15001 gaagttgtta aacaagccgc ggagctatta agaacaagtg gtttaaactt ctacggtaat
    15061 atcgagggtg atgatattta taagggaact accgatgtgg tcgtgtgtga tggctttgtg
    15121 gggaatgtgg ctttaaaaac ttctgaaggg gtggcgcaaa tgttggccac ttttttgcgc
    15181 caggagttta aacgaaatat ttttacaaaa cttgtcggta tagttgcatc accagtcatc
    15241 aatgctttta agcagcgggt tgatcatcgt cgatataatg gggctagttt gttgggtctt
    15301 agggggattg tgattaagag tcatggttct gctgataaat atgcttttac tgcagccatt
    15361 aggcgagcaa ctgatgaagt acgtggaggt atgctaaagc atattagcga gcgagtggct
    15421 gaattacagc aaggcattgg aaaaaataat taaattaagc tcttattgag tgataaaaat
    15481 ggcaatgtat tcaaaaatcg ctggaactgg tagctattta ccagagaaaa ttctgactaa
    15541 tattgagtta gaaagtatcg tggatacaag tgatgagtgg atacaaactc gaactggcat
    15601 tgctcagcga catattgcag cagatgatca aatggcaagc gaccttgctt tagcggccag
    15661 ccgtcgagca ctagatgcag caaatcttaa agcacaggat atcgatctga tcatccttgc
    15721 tacgactacc ccagatatga ttttcccaag tacggcctgt attttgcaag aaaaactcgg
    15781 tattaccgat ggtcctgcat ttgatgtaca agctgtctgc agtggtttta tatatgcact
    15841 ggcaacagcg gatatgtttg ttagctcggg aaaaagtaag aatgcccttg tggttggtac
    15901 ggagatatat tcgcgtatta ttgattggaa agatcggagt acatgcgtat tgtttggtga
    15961 tggtgccggt gcggttgtgt tgtcgcaaag tgaacaaccg gggatactct caagccattt
    16021 acacgcgaat gggggttaca gcaagatact agctgcaccc ggcagtattt ctggaggaaa
    16081 gattcaagga acgcctttta ttgcaatgga agggaacacg gtattcaagt ttgcggttaa
    16141 agcactggaa gaagcggcaa ttgaatcttt ggcagctaat aatttgcaag cctctgatat
    16201 cgattggttt ataccgcatc aagctaatat tcgtatcatt aaatcgacag ctcacaagct
    16261 tggaataccg atggagaaag tagtcacaac ggtagatcga catggtaata cttcagctgc
    16321 atccgtgccc cttgcattag atcttgcagt ccgtgatgga cgtattcagc cagatcacta
    16381 tgttcttcta gaaggagtag gtggcggttt tacatgggga tctatattgc ttcagtggtg
    16441 aaaaaatgat tattgctttt acgtttcctg gacaaggttc ccaatcggtt ggcatgctga
    16501 atggctacag tgatctgcca attattaaac agacatttga tgaaggctct gaaattctag
    16561 gctatgactg ttttgaattg gcaaaacatg gtccagtaga acaacttaat ttgacgattc
    16621 acactcaacc aatcatgttg atggcgggta tcgcagttta tcgagcctgg aaatctttag
    16681 gggggggaag tccagattac ttagcaggac atagcctggg cgaatatacg gcattagtgg
    16741 cagcagaagc gttagctttt agagatgcca ttaagttagt gcgataccgt gcagaggtca
    16801 tgcaggaatg cgtgccaaac ggaatgggtg caatggccgc tataattgga ttagaggatg
    16861 atgctgtcaa ggcaatttgt aatcaaattg ttggtgataa tgagaaggtt gtacttgagc
    16921 ctgccaattt taattcgcct ggtcaaatcg tgatcgctgg acataaagct gcggtattag
    16981 aaggaatggc gctagctaaa tcgaaagggg caaaattagt taccctgctt cctttgagca
    17041 tcccctcaca ttgttctctg atgtatcctg ccgctgaaaa atttcagaaa ttgcttgagc
    17101 aaattgattt ttatagcccg aaaataccag ttatacataa tgctgatgta caacaacatc
    17161 aacagagtaa tgaaatcaaa aaaatattaa tgcagcaaat ccatagtccg gtgcactggg
    17221 ttaataccat tcaatctttt gtttctcagg gagtgacgca tattgttgaa tgtgggcctg
    17281 gaaaagtact ctctggcctt acaaaacgca ttgataaaac cccgaccaat tattcactaa
    17341 ccgatagtga atcgctccag caagcgattg agaaattgaa tcaacctgct ttttgataag
    17401 aatgtatgga aaaaaaattg cttgaaggaa atattgcttt tgtgactgga gcaagccgtg
    17461 gaataggtat ggctattacg ttggagcttg ctaaacaagg tgcaattgtc attggaacag
    17521 caacaaccca gaatggtgct gatggtataa cgcgccaatt agaagagatc agtgcccctg
    17581 gaatgggtgt tgtcatgaat gtcaatgatg ttgatcaaat tggtaaggca tttgaaatga
    17641 ccaataaaca atttggcgat atctcaatat tggttaacaa tgccggtatc acgcgagata
    17701 atttactggt tcgaatgaaa gatgaagagt gggatgaagt catgacaacc aatcttaaat
    17761 caatttttta tctttccaaa atagcactcc gagccatgat gaaagcgcgt tctggacgaa
    17821 ttattaatat ttcttctgtt gtcggagcga ctggtaatcc ggggcaggct aattatgcag
    17881 catcaaaggc tggtatgatt ggttttacaa aatctctagc gagagaggtc ggaagtagaa
    17941 atgttacgat caactgtgta gctcctggtt ttattgacac agacatgaca agttcgttaa
    18001 cgactgagca gcgccttaat ttacttaagc aaatacctct gggtcggctg ggtcagtcag
    18061 cggatattgc agcagcggtg gtgtttttag cttcgcctgc tgcaagctat ataactggaa
    18121 ctacgttgca tgttaatggc ggaatgttta tggagtaatc acgaaatctt attgataaac
    18181 ctgatacgat taggtttggt tttttaaaaa acgaaatttt gttaaaatca cttagttaat
    18241 tacctcacaa aaggatacca gatggataat acagatgtag aacaaagaat tagaaaaatt
    18301 gttgctgagc agctcggtgt gaacgaaagt gatgtaaaaa acgaatcctc ttttgttaac
    18361 gacttaggcg ctgattccct cgatacagtt gaactggtga tggcacttga agaagaattt
    18421 gattgtgaaa ttccagatga agaggctgaa aaaatcaaca ccgtacaaga agcaattgat
    18481 tacgtaaatt cacattcctc caattgatat tatttatata gctttttctt taggagatac
    18541 atttgtcaaa acgcagggtg gtagtaacgg ggttaggtat aatatcgcct gtcggcaata
    18601 atgtatcgga tgcttggcaa aatatcgttt cagggaattc aggtatcacg cgtataaatc
    18661 gttttgacgc ttcaaatttt tcttcccaaa ttgcaggtga agtcaaaaat tttgatatta
    18721 ctcattattt atcagcgaaa gatgcacgta ggatggatat ttttatacac tatggcatgg
    18781 ctgctggtat tcaggcgatt agagatg
//
LOCUS       DAOQKQ010000030        18296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_1373_length_18296_cov_6.205581, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000030 DAOQKQ010000000
VERSION     DAOQKQ010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 18296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18296
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1373_length_18296_cov_6.205581"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="PKZ49_03280"
     CDS             <1..910
                     /locus_tag="PKZ49_03280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HND35614.1"
                     /translation="SRPNILLLHAHFCHGATTVAWFVSQMTGLPFSFTAHAKDIYQKQ
                     LNPGRLLEQKLAAAQFVTTCTKTNHDYLTALHDENCPIHAVYHGINTKKFIPRTNYQL
                     NVGQPLILAVGRHVEKKGFVYLIEACAKLHLANIDFQCLIIGESGDQTDMIKTTIQNK
                     KLESQVVLRNAVTQEELRELYQQATIFALPCVITKDGDRDGIPNVMAEAMATGLPIVS
                     TPISGIPELVEDQVNGLLVPPRDVEGLAGALKLLLADEMLRHKLGKAARQTICEQFDA
                     TVTIRSLQQLFQQALTYSKQHKPDNE"
     gene            903..2819
                     /locus_tag="PKZ49_03285"
     CDS             903..2819
                     /locus_tag="PKZ49_03285"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HND35615.1"
                     /translation="MNSPSKPTLIWDTSYDALSPAQRLNYFSERQHIHFFPVLDREMT
                     EEAFVDDVLDNRFNFNGEVFLLTDPIDWLCNPSPDIEWHILLHKFYYAPGLGKAWVET
                     QDRRYLEKWVELITSWMEQTPIDFIAPDVTGRRIQNWIYSWYYFVASNRTERIDPQFH
                     DRFLDSIQKQVNYLCTHLAPARNHRTIALYSIFLASIVFPETTDAKHWQDFSLREIYQ
                     NVLQDLLPDGVQCELSTDYHHLVLKNYLAIRLLAKLNQITIQPEFDERLNRALDFAMF
                     AHKPDGEVPSFSDGDVRSFEDCLLQGASLYQREDLLFVGTRGQQGIAPSQRNAGFDVS
                     GYYILRSGWGQENTSYQDERYLMIDCGPLGEGNHGHFDALSFEAAAFGRSLIVDPARY
                     TYHEAKEVNWRVKFRSTESHNTVLVDGKNQTRYLAGLKRYKIKGPQPVTQLHAFQTQR
                     RVDYFHGSTRSSEYDALHHRQIAFIQGEYWIIVDSLYSASWHEYEQLFHLSTHAFQSV
                     QTIQDENCCQVITPNLVIATPANQNHQLIVETGYVSRRYGEKKEAPVLRFRKRAKNTR
                     FVSVLYPFKDGNPDIYVSIVEMNAGNHFEVNCLVYQKSGLGFADSCQFPKPELVTGEH
                     FSWDRKRIHSGDRI"
     gene            2822..4234
                     /locus_tag="PKZ49_03290"
     CDS             2822..4234
                     /locus_tag="PKZ49_03290"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HND35616.1"
                     /translation="MAMVGHENKELPASDYYDACLLHMASLLDADLMADVLKAQLFPF
                     QPETFVVVSCKLVRVKYKPLKNCLAVYDFTVEHILTGEKRHFYITCRTYEFQGSRRRF
                     DRAVRQHDRNPSDFPAIFHVPELEIIGWVFPCESKLLSLPFISHAHYLKHVVLPRLIE
                     SLCGKDWQIVKFSSTVMHYVPEHTCTIRVELSLTRQGQKLAKTVFGKCYYNEAGEQTF
                     SIMRKLHESSLNGITPLRFPAPLFYDAELKLLWQEGLSGQTLRDATAGVESRRELLPQ
                     AARAIALFHRIPVDHQLSVVKITDMLTTLVNRMEVLSHVTPLDRRKLNQVILSLHRTV
                     PILAPTEIVTLHGDVHPQNLFLQNQSVAFIDLDSVVAGPAYHDVASWLCGMLYWGAMQ
                     HRSLQTLLPELGFFVTNYNQYAPFRVDEQDLSWFIAMALINERIFRCITRFKSGRLQI
                     INELLDWAEQFACGQQRIAI"
     gene            4261..5982
                     /locus_tag="PKZ49_03295"
     CDS             4261..5982
                     /locus_tag="PKZ49_03295"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HND35617.1"
                     /translation="MKRIFQIFLPMLWEYRKALFIAYLWSFIAVAAIILSPWPLKFVI
                     DYVLIDKALPDWLTHLTLGLSPVAQVLLLALVLALISAIGAFASATEKNRNAKIREQL
                     DLDVRERLLNHIQSVPAILFDNQKSGELVLRLVDDGQKVVRLLTKTTPVIFRYLTTTL
                     FAFVAMCMVSPFLGILGGLIVLMLSILVRRYAGPLQEASRFKRKTEGDVSALAQEIIK
                     GLANTQAHGMEHEVSQRFQSKTRISKRAGVEETRVAVLMELSMQLANGLAVSLVIGGG
                     GYLVLQDQLSLGSLTVCLAYMTQLLKPVEKINELAATVSRALVRGEYLIHLFDQFSPI
                     PDRPNATHLLQPKGIIRLEDVGYAYSSRDNNQSQPVLQHVNLVLEPNKLVVLVGPSGS
                     GKSTLLKLLLRMMDPTEGTLLFDGVPYQNIQLKHLRRQFAVMMQRNHVFSGTLRTHFF
                     IPGQDIDDQKIWNALELVAMADYVKQLPQELDSPLKEDGLNLSGGQRARLDLARTFLL
                     DRPVLLLDEPLANVDPESSQIIMSALDRIRVGRSCLVATHQMEMAKRADQIVQLKNGK
                     LLVGGFA"
     gene            6013..7188
                     /locus_tag="PKZ49_03300"
     CDS             6013..7188
                     /locus_tag="PKZ49_03300"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HND35618.1"
                     /translation="MKKPRLLFYCQHSMGMGHLVRSFRLAEVLVDAFDVVFLNGGPIP
                     SGISPPVDITLINLPALGMSEQDHLLFSHDTRYSVDEAKSIRQKMVLQAFDHYQPEVI
                     LIELFPFGRKKFADELIPLLSNAHQKNPKPLVLSSLRDILVQKRADQHRFENQVSQTL
                     NRYFHAVLLHCDPAFATLSEYFKPTIALQVPVFNTGFVAPPVADPGYSHNPGIIVSAG
                     GGIVGGELYRTAIHAQKLIWKEIAIPMTLVAGPFLPEEEWQHLVKIGHDVGGLTLHRT
                     VSGLMSLLSPRNISVSQCGYNTAMELLQSGVKALVVPFCTAHEDEQMSRAQKLMSAGA
                     VKLLHPALMTDKTLAGAILELWQAEKFQSKPLNTQGALNTLEIILRLRRTEYGGEVQ"
     gene            7185..7943
                     /locus_tag="PKZ49_03305"
     CDS             7185..7943
                     /locus_tag="PKZ49_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35619.1"
                     /translation="MNQTWLLPLQQALERLDRSVTFFYRDDDAGWDTPALLRLLDLFD
                     DLQLPIDLAVIPKAITPELAAILNTRLVQCHNLIGLHQHGYAHVNHQTVGRKCEFGEM
                     RQFDQQLHDIVAGKEILHDYFGNRIDPIFTPPWNRCSQTTLDVLNTLPFTVLSRAEKA
                     TPFSLNQMVEISIHIDWLKRTHGERWSREQVIRLIAEKILMNSSIGIMLHHQEMDQED
                     LIAFKQFARLIERHPNIRHKRMRDLTDQIESAMR"
     gene            8010..9557
                     /locus_tag="PKZ49_03310"
     CDS             8010..9557
                     /locus_tag="PKZ49_03310"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HND35620.1"
                     /translation="MRTDKSSHLSSGKRIALYPGAFRPPHAAHMHAVQYLANHPDIDE
                     VVIIISGRFRHIPGTDLALGPEVAEAIWQIYLEGLTNVRVVIASQTAVSYALDYFDKV
                     LPDQTLLFCIGEADLQSGDSRFYQIHQRAKETGISASIIMVPTGDLKIRSTALRASLV
                     DVENGSQIFMDCLPNQLNLAQQMQVWAICQNGWKTVIEVTRDRIQKVLSYSMQVRLAT
                     QLLPGKNRLDPVFFTQTEQGEHWVIKFAGSSLEVENNAQTDRPKSRNRLSVERLALKW
                     LNHHFRSGIKFPETLFWMKKEKMLIMTRLGFTGHSLAHRLSEGYFDRWVLNQVNAFLA
                     DNYHADSPAEAFWGDQSDDKYQWQHILEEHLKSARSHFFNNDFDQYLRQLQIKSLAAT
                     CHQLVHLDLQPKHILLNDSEIAIVDWESSSTFGDPAFDLGKLLGHFLFYAIQAQQTES
                     IVCLIEEVVLTFQSRTIGTCDHDFISRVYGFIGSTLLSLGQNIKESQFKQLTACVRKI
                     EFTYYSN"
     gene            complement(9602..10507)
                     /gene="galU"
                     /locus_tag="PKZ49_03315"
     CDS             complement(9602..10507)
                     /gene="galU"
                     /locus_tag="PKZ49_03315"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HND35621.1"
                     /translation="MKKITKAIFPVAGMGTRFLPATKASPKEMMPVIDKPLIQYAVEE
                     AVSAGITEMIFITGRNKRSIEDHFDKAYELESALELRGKQETLAELRSILPKHISCIY
                     IRQAEALGLGHAILCARPIICNEPFAVLLADDLIDAEKPVMQQMVEIYANNPYSILGI
                     QNVDPHKTNEYGIVECDLGPKNLHKITNMIEKPEPHNAPSTLGIVGRYILNPRIFRHL
                     EKITPGAIGEIQLTDAIASLRLEESVLAYQFAGTRHDCGSKLGYLKATIALALKHPEL
                     GKELSNYLSTQHALNLATHEDYTPA"
     gene            complement(10577..11596)
                     /locus_tag="PKZ49_03320"
     CDS             complement(10577..11596)
                     /locus_tag="PKZ49_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HND35622.1"
                     /translation="MKTLVTGAAGFIGMHVTKKLLEDQFDVIGLDNLNDYYDIKLKED
                     RLKQLQAFKNFRFIALDIADKIAMEKIFNEEKFQLVINLAAQAGVRYSLKNPYAYINS
                     NIMGFMNILEGCRHHPVSHLVYASSSSVYGINTKMPFSVTDNVDHPASLYAATKKSNE
                     LMAHTYSHLFGIPTTGLRFFTVYGPWGRPDMSPSLFAHAIFQEKPIDVFNHGKMQRDF
                     TYIDDIVEGVIRILKKPATADPLYSRINPNPARSDAPYAIYNIGSHTPILLDDFIGEL
                     ETVIGKKAIKHLLPMQPGDVEATYASVADLKKAVGFEPKTSLSDGIKQFIDWFRKYYG
                     YTHQN"
     gene            complement(11608..12930)
                     /locus_tag="PKZ49_03325"
     CDS             complement(11608..12930)
                     /locus_tag="PKZ49_03325"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HND35623.1"
                     /translation="MKISVIGTGYVGLVTGACLAEMGNDVMCVDIDEKKIALLQEGKI
                     PIYEPGLESLVERNQAYGRLSFTTDIKKSTQHGLAQFIAVGTPPDEDGSADLQHVMAV
                     ARAIAEHMQEYKLIIDKSTVPVGTAKKVQAVITETLYRQGKVIDCSVVSNPEFLKEGN
                     AVEDFMKPERIVVGADDRKAIGLMKILYAPFNHNHNRLVLMDTASAELTKYAANAMLA
                     TRISFMNELALLAEKLGADIEHVRHGIGSDSRIGHHFLYAGCGYGGSCFPKDVQALQH
                     IAKEQDHELKILGAVELTNNRQKKVLLEKIVTKFNGNLNGKRIAIWGLAFKPNTDDMR
                     EAPSRILIEGLWSHNATVVAYDPAAMEQAKRIFGNNPRLQLADTPYSALENADALAIV
                     TEWKAFRTPDFSLIKNKLKQPIIFDGRNMYDPEAVRVEGIEYYSIGRN"
     gene            complement(12995..14419)
                     /locus_tag="PKZ49_03330"
     CDS             complement(12995..14419)
                     /locus_tag="PKZ49_03330"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HND35624.1"
                     /translation="MSISCESFSNDQIRRGRQNTVIDDVDSRAMGLGGDDERLRPKAT
                     RHFQYGNTDISFTGAATLLDFSFENNFSFRENDAVDQFRFRPLVDLNTIFDFPKGYSL
                     FTEFRLRDSIKLQDDTQPSNFFELQVREFFLTAPILETIPIDLTLGRQQFFDLRRWYF
                     TDRMDAARLSAKSGPFDLFTAISTPVLVTNHSLQVFDNIAIKKEQMDILFNLDYALTE
                     RSFISGYLILGNKHQDRIEPGLLRNENPMWSGIRFIGDEKFKLASSESEFLSNLLKPR
                     IQYWLDAAFVSGSADSKKISGLGIDLGVTAIARKFQAKPYVTFGYAYGSGDSNPENDT
                     DHNFRQTGFQANSGRFGGVVNFDYYGVLYNPELRNMHILTAALGIRPTERSSIELVYH
                     RYAQDHAFARLPRDLRDGPITFSRNPDGIHSHLGDELDLVLGYRGINNVRLRSRTGYF
                     LPGAAYGEQRGSAFFTRLDVQFFF"
     gene            15309..16106
                     /locus_tag="PKZ49_03335"
     CDS             15309..16106
                     /locus_tag="PKZ49_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35625.1"
                     /translation="MSSRDDDNSSGRSSDDGINHDINDDHGRGRGSDDGVNHDINDDH
                     GGRRNDDRSSDDNSSGGINVSGNHKAFKFEIVNGQVTAVFEVKDGELKSKSLTDGGRK
                     SYTVDGNDVIRTEIKPFGTEITRYSDDNGDGIYLRTSEQWQVSSNSNGVTPKFTDIIH
                     FSHTSGDDRIAVRGGEDCRGGNGADDFVVREAAHLHIGDFSSREGDLLIFDTGLGLTS
                     RDQLASYVTDTHHDGQNFIVNFGSDVSITLVGVQADQVSWNDVSVLS"
     gene            16184..18004
                     /locus_tag="PKZ49_03340"
     CDS             16184..18004
                     /locus_tag="PKZ49_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper oxidase"
                     /protein_id="HND35626.1"
                     /translation="MLYRLNKAMQSMAYGIVSLFCLVLSTGSVLAKDNEVSFALTDNA
                     GRWFDTGTAIAGNRSIAVATPGVRVNFSGNSNTVHTRTSLIYPTGAAGMPFNTEPKKG
                     GDSITLTTPGLYVFTCSIHPYMFGAVIVDDPATSGLDLGASISLINGITVPTSSDLAT
                     RLLRTFFIATNPANWQNYASNAPWHITYPNVDVRVDIGVVNLPAVLNARYGNDISLPA
                     LSNPATQAVGEVWVATQFETTSGKSKPGTVTAVDGSSWQVTRKVALPSINMNNPHNMW
                     TDKDQNVIYVTQWFDKKLTVYNRQTGALIRNVSVGEAPAHVMTLTDNDRIHVTNNGDT
                     RTDSVMELSPLATNVLRRIDIGRGNPHAHWMSHDGKFMVTPNVLTGDTSQYNFRTNDV
                     EAILPVSGPLSHPLATGMMPDASKYYVANLLDSTITVVDMESHNVLKHINLIANYHPV
                     TGAITGPVGALPIQTPVSPNGKNMVTANTLTGTITILDTATDEIVAMLPCDPGCHGVQ
                     YGAKLGGGYYAYVTSKFSNRMLVVDPDPNNDGNPADAAIVGSIGLFATNRTLKDATIS
                     GNAGMGGQGILPIPVVYNGWVQKLPSNWSNLLTPAQRNPW"
BASE COUNT         5110 a         3639 c         4117 g         5430 t
ORIGIN      
        1 aagtcgtcct aatattttgt tgttgcatgc gcatttctgt catggcgcaa cgactgtggc
       61 ttggtttgta agccaaatga cgggactacc ttttagcttt actgcccatg ctaaggatat
      121 ttatcagaag cagctcaatc ccgggcgact cttagagcaa aaattagcag ccgctcaatt
      181 tgtcacgaca tgcacaaaaa ccaatcatga ctatttgacg gcgctgcatg atgagaattg
      241 ccctattcat gctgtgtacc atgggatcaa taccaaaaaa tttattccac gaactaatta
      301 ccaattaaat gtggggcagc cgctaatttt agctgtaggg cgtcatgtag agaaaaaagg
      361 atttgtctat ttaatcgagg cgtgcgcgaa acttcatctg gcaaatattg attttcaatg
      421 tttgattatc ggagagtccg gtgatcaaac agacatgatc aaaacgacaa ttcagaataa
      481 gaaactagaa tcccaagtcg tgctcagaaa cgcagtgact caagaggaac tccgggaact
      541 ttatcagcaa gcaacgatat ttgcgttgcc ctgcgtcatt actaaagatg gggatcgaga
      601 cggaatacct aatgtgatgg cggaagccat ggcgactggg ctgccaatcg tttctacccc
      661 tatatcgggt attccagagc tagtggaaga ccaagtcaac ggtttactcg ttccgcctag
      721 agatgtggaa ggactagccg gcgcactgaa attactgtta gctgatgaaa tgctgcggca
      781 taagctagga aaagcggcac ggcaaacgat ttgtgagcag tttgacgcga ctgtaaccat
      841 tcgctcgttg cagcaactat ttcaacaagc actaacttat tcaaaacagc ataagccgga
      901 taatgaatag ccccagtaaa cctaccttga tctgggatac ttcctatgat gcactgagtc
      961 ccgcgcagcg tttaaattat tttagtgaaa ggcagcatat ccattttttt cctgtgctgg
     1021 atagagaaat gaccgaggag gcctttgttg atgatgttct agacaatcgc ttcaatttta
     1081 atggagaggt ctttttattg accgacccaa tagactggct gtgcaaccca agtccagata
     1141 tagaatggca tattctatta cacaagtttt attatgcgcc agggcttggc aaagcatggg
     1201 ttgagaccca agacagaaga tatttagaaa aatgggttga actaataaca agttggatgg
     1261 agcagactcc aattgatttt atcgctcctg atgtaacagg tcgtcgcatt caaaactgga
     1321 tctacagttg gtactatttt gttgcttcaa atcggactga gcggattgat ccacagtttc
     1381 atgaccggtt tcttgattca atccaaaaac aggtcaacta cttgtgcact catctcgctc
     1441 cagcccgaaa tcaccgaact attgcgctat attcaatttt tcttgcttcg attgttttcc
     1501 ctgaaactac tgatgcaaag cattggcaag atttttcgct gcgagaaata tatcaaaatg
     1561 tgttgcaaga cttattacct gatggtgtgc aatgtgaatt gtcgacagat tatcatcact
     1621 tggtgttgaa aaactatcta gccattcgac tgctggcaaa actaaatcaa attactatcc
     1681 aaccagagtt tgacgagcgc ttgaatcgtg ctttggattt tgcgatgttt gcacataaac
     1741 ccgatgggga ggttccttct tttagtgatg gtgatgttag aagttttgag gattgcttgc
     1801 tccaaggggc atcattgtat cagagagaag atttgctatt tgtcggcact cgggggcagc
     1861 aagggatagc gccttctcaa cggaatgctg gttttgatgt cagtgggtac tatattctta
     1921 gaagtgggtg gggtcaggaa aatacttcct atcaggatga gcgttactta atgatagatt
     1981 gcggtcccct tggtgaaggc aatcatgggc actttgatgc attaagtttc gaagcagcag
     2041 cttttggtcg ctcactgatc gttgatcctg cacgatatac ctatcacgag gcaaaagagg
     2101 ttaactggcg ggtaaaattt cgtagcacag agtcgcacaa cacggtgcta gtcgatggca
     2161 agaatcagac ccgatatctt gcgggtttga agcgatacaa gataaaaggt ccgcaaccag
     2221 ttacccaact gcatgcattt caaactcagc gtcgtgttga ttattttcat ggttcgacac
     2281 gaagttctga gtacgatgca ctgcatcata gacaaattgc atttattcaa ggtgaatact
     2341 ggattattgt tgatagcttg tacagcgcat cttggcacga atatgaacaa ctctttcact
     2401 tatctacgca tgcattccag tctgttcaaa ccattcaaga tgagaattgc tgtcaagtca
     2461 ttacacctaa tttggtgatt gctacaccag caaaccagaa tcatcaatta attgtagaaa
     2521 caggatatgt ttctaggcgt tatggtgaga aaaaagaagc gcctgttcta agatttagaa
     2581 agcgcgcaaa aaatacgcgg tttgtatcag tgctatatcc ctttaaagac ggtaatccgg
     2641 atatttatgt ttccatcgtt gaaatgaatg caggtaatca ctttgaagtg aattgcctgg
     2701 tttatcaaaa aagtgggctt ggttttgctg atagttgtca attccctaaa ccagaattgg
     2761 ttactggaga gcattttagc tgggatagaa agcgcataca ctcaggagac cggatttgat
     2821 tatggcaatg gttggacatg agaataaaga actacctgct tctgattatt atgatgcttg
     2881 tcttctgcac atggcctcat tgcttgatgc cgatttaatg gccgatgtgt tgaaagcgca
     2941 attgtttcca tttcaaccag aaacatttgt ggttgtctca tgtaagctcg ttcgcgttaa
     3001 gtataaaccg cttaaaaatt gtcttgcggt ttatgatttt actgttgagc atatattgac
     3061 tggggagaaa cgacatttct acatcacctg cagaacctac gagttccaag ggtctaggcg
     3121 acgttttgat agagcggtaa ggcagcatga tagaaaccca tctgattttc cagcaatatt
     3181 tcatgtccct gaactagaaa tcattggttg ggttttcccc tgcgaatcga aactattgag
     3241 cttgccattt ataagccatg cacattacct gaagcacgta gtgttaccgc gactgattga
     3301 gtccctgtgc ggtaaagatt ggcagatcgt taaattttca agtacggtaa tgcactatgt
     3361 gccagagcat acctgcacca ttcgtgttga gctatcgttg actaggcaag gccagaagct
     3421 tgctaaaaca gtatttggta aatgttacta caacgaggca ggggagcaaa ctttcagtat
     3481 catgagaaag ctacacgaat cttccctcaa cggaatcacg ccacttcgct tccccgcacc
     3541 actattttat gatgcagagt tgaagctact ttggcaagaa ggtttaagtg gccagaccct
     3601 tcgcgatgca acggctggtg tggagtcacg ccgggagtta ttgccacaag cggcaagggc
     3661 aatcgcatta tttcatcgca ttccagtaga tcatcagctt tcggtggtca agattactga
     3721 catgcttaca acgttggtta atcgtatgga agttttgagt catgtaacgc cactcgatag
     3781 acgcaaacta aaccaggtta ttttatcgtt acaccgcaca gtacctatac tggcgcctac
     3841 tgagattgtt actttgcatg gggatgtgca tccacagaat ttatttcttc agaaccaatc
     3901 ggttgctttt attgatctcg atagcgttgt tgctgggcca gcttaccatg acgttgcaag
     3961 ctggctttgt gggatgcttt actggggtgc gatgcagcat agatctctgc aaacgctgct
     4021 gccagagtta ggtttttttg taacgaacta taaccagtat gcaccatttc gagttgatga
     4081 gcaggatctg agctggttta ttgcgatggc acttattaat gagcggattt ttcggtgtat
     4141 aacacggttt aaatctggcc ggttacaaat aatcaatgaa cttctagact gggctgaaca
     4201 atttgcatgt ggtcagcagc gaatcgcaat atgaaaaaga atgatcacgc gcagtctagt
     4261 ttgaaaagaa tatttcaaat ttttctaccc atgttatggg agtatagaaa agccttattc
     4321 atcgcatact tatggagttt cattgcagtt gccgcgatta ttctatcccc ctggccacta
     4381 aaatttgtta ttgattatgt gctcatcgat aaggcgttac ctgattggtt aactcattta
     4441 acgctaggat tatctcctgt tgcacaagta ttgctacttg cgttagtgct cgcgcttata
     4501 tcagcgatcg gtgcttttgc atctgcaaca gaaaagaatc gaaatgcaaa aatacgtgaa
     4561 caacttgatc tagatgttcg tgaacgatta ctcaatcata ttcaaagcgt tcctgccatt
     4621 ctgtttgata atcagaaaag tggggagctc gttcttcggt tagtagacga tggtcagaaa
     4681 gttgtcagat tattgactaa aacaacgccc gtcatttttc gctaccttac gacaacacta
     4741 tttgcttttg tggcgatgtg catggtttcg ccattcctag gaatactagg gggactcatt
     4801 gtcttaatgt tgtcgatatt ggtcagacga tatgcagggc cacttcagga agcgtcgcgt
     4861 tttaaacgta agactgaagg agacgtgtct gcattagccc aagagataat taagggactg
     4921 gcaaatacac aagctcatgg tatggaacat gaagtcagtc agcgatttca aagtaaaacg
     4981 cggatcagta agcgtgctgg cgtcgaagag acacgtgttg ccgtattgat ggaactcagt
     5041 atgcagttgg ccaatgggct ggcagtttcc ctcgtcattg gaggaggtgg gtatttggtg
     5101 ctccaggatc aactttcact tggcagtttg acagtatgtt tggcttacat gacacaatta
     5161 ttaaagccgg tggaaaaaat caatgagctg gctgccactg tttctcgtgc cttggttcgt
     5221 ggagagtacc tgattcattt gtttgatcaa ttttcaccaa taccagatcg tcctaatgcg
     5281 actcatctct tgcagcctaa agggataatt cgcctagagg atgttggtta tgcctattca
     5341 tcgcgagata ataaccaatc gcaacctgtt ttgcagcatg taaaccttgt attagagccg
     5401 aataaactcg ttgtattggt cgggcccagt ggtagtggga aaagtacatt actgaagtta
     5461 ttacttcgta tgatggatcc gactgaaggt actttgcttt tcgatggagt tccttaccag
     5521 aatatccagc ttaaacattt gcggcggcag tttgctgtca tgatgcagcg taatcatgtt
     5581 ttttctggaa cattgcgaac ccattttttc attccagggc aggatatcga cgatcagaaa
     5641 atctggaatg ccctagagtt agttgctatg gcggactatg ttaagcagct accacaagag
     5701 ttagactccc cgcttaaaga ggatgggctt aatctgtcgg gtgggcagcg cgctaggctc
     5761 gatttagcgc gtactttctt actcgacagg cccgtgttat tgctcgatga accgcttgcc
     5821 aatgttgatc cagagtccag tcaaattatt atgagcgcgt tggatcgtat tcgagtcggt
     5881 cgcagttgtc ttgttgcaac gcaccaaatg gaaatggcta aacgtgcaga tcaaattgtc
     5941 cagctgaaga acggcaaatt gttggttggt ggtttcgcat gactctgctt tctttaattt
     6001 aagaaactta atgtgaaaaa accaaggctg ctattttatt gccagcattc catgggaatg
     6061 ggacacttag ttcgttcttt cagacttgct gaagtattgg tagacgcctt tgatgttgtt
     6121 tttctcaatg gaggcccgat tccatcgggt attagccccc ctgtcgatat aacattgata
     6181 aaccttcccg cgttgggaat gtctgagcag gatcatttgt tgttcagcca cgatacgcgt
     6241 tatagcgtag atgaggcaaa atcgattcgg cagaaaatgg ttctgcaagc gttcgatcat
     6301 tatcaaccgg aggtaattct aattgagcta tttccttttg ggcgaaagaa atttgcggac
     6361 gaattgattc ctttgctatc gaatgcccat caaaagaatc cgaaaccact ggtgttgtct
     6421 agcctgcgtg atatcttggt acagaagcgt gctgaccagc atcgatttga aaaccaagtc
     6481 agccaaactt tgaatcgtta ctttcatgca gttctgcttc attgcgaccc tgcttttgcc
     6541 acgttaagcg aatacttcaa gcctacgatt gcactccagg ttcctgtttt taatacgggt
     6601 ttcgtcgctc ctcctgtggc tgatccgggt tactcgcaca atcctggaat cattgtttct
     6661 gccggaggag gcattgtagg aggagagtta tatcgaactg caattcatgc acagaaacta
     6721 atttggaaag aaatcgcaat tccaatgacg ctggttgctg gcccatttct tcctgaggag
     6781 gaatggcagc atctcgtgaa aatcggacac gatgttggtg ggctaacgct tcatcgaacg
     6841 gtatcagggt tgatgtcgtt gctatctccc cgcaatattt cagtcagcca gtgtggctat
     6901 aacacggcga tggagctact ccaatctggt gtgaaagcat tagtggttcc attttgtacc
     6961 gcccatgaag atgaacaaat gagtcgtgcg cagaagctga tgagcgcggg agctgttaag
     7021 ctgcttcacc cggccctgat gaccgacaaa acacttgctg gagctatctt ggagttgtgg
     7081 caagcggaaa aatttcagag taagcccctc aatactcagg gggcgctaaa tacgcttgag
     7141 attattctca ggctgagacg aacagaatat gggggcgaag tacaatgaat cagacatggc
     7201 tgcttccact acaacaagcg ctggagcgat tggatcgatc ggttactttc ttttatcgtg
     7261 atgatgatgc gggttgggac actccggcat tattgagatt acttgatcta tttgatgatc
     7321 tgcagttgcc tattgatctc gcggtaatcc ctaaagcgat tactcctgag ctggcagcaa
     7381 tacttaatac caggcttgtg caatgccata atcttattgg attacatcaa catggctatg
     7441 cgcatgtcaa tcatcaaacg gtaggcagaa aatgtgaatt tggcgaaatg cggcaatttg
     7501 accagcagct gcatgacatc gtagcaggta aagaaatctt gcatgattat tttggtaatc
     7561 gcatcgatcc catttttaca ccaccttgga atcgctgtag tcaaacgacc ctggatgtgc
     7621 tcaatacatt accatttaca gttttgtctc gagcagaaaa agctactccg ttttccctta
     7681 atcagatggt tgaaatatcg attcatatcg attggctaaa gcgaactcat ggggaacgtt
     7741 ggagtcgtga gcaagttata agactgatcg cagaaaaaat tttaatgaat tcaagcattg
     7801 gaatcatgct gcatcaccag gaaatggatc aagaagattt aatcgccttt aagcaatttg
     7861 ctcggttgat agagcgacat cccaatattc ggcacaagcg gatgcgtgac ttgactgacc
     7921 agatagaatc cgcaatgaga tgagtagatt gtattttggt ctcgggctga aagtgggcac
     7981 cgtgctatcc ctatttgatc gactaatcca tgagaaccga taaatcatcg catttgtctt
     8041 caggtaaacg gattgctctg tatccaggag catttcgtcc gccccatgca gcacatatgc
     8101 atgcagttca atatctggcg aatcatccag atattgatga agtcgtaatt atcatttctg
     8161 ggaggttccg gcatataccc ggcactgatt tagcgctagg tcctgaggta gcagaagcga
     8221 tctggcagat ttatcttgaa gggttaacca atgtgcgagt cgtaattgct agccagactg
     8281 ctgtcagtta tgcgctcgat tattttgata aggtattgcc agatcagacc ttactttttt
     8341 gtataggaga agcggacctt caatctggcg attcgcgatt ttatcaaatc catcagcgag
     8401 caaaggaaac aggtatctct gcatcgatca tcatggtgcc cacgggagat ttgaagattc
     8461 gttctacagc cttgcgtgca tctctagtag atgttgagaa tgggagtcag atattcatgg
     8521 attgcctacc taatcaattg aatcttgctc aacagatgca agtttgggcg atttgccaaa
     8581 atggctggaa aactgtgatt gaagttacac gcgatcgtat acagaaagta ttatcctatt
     8641 ccatgcaagt aaggctggct actcaattgc ttccaggaaa gaatcgactg gatccagtat
     8701 tctttaccca aacagagcaa ggtgaacatt gggttattaa gtttgcaggt agttcgcttg
     8761 aggtagaaaa caatgcccaa actgatcgac caaaatcgcg caatagattg agcgtggaga
     8821 ggctagcgct gaaatggttg aatcatcatt ttcgatcagg catcaaattt ccagaaacgc
     8881 tcttttggat gaagaaagaa aaaatgctga taatgactcg attggggttt acaggtcatt
     8941 cacttgcaca tcgattgtct gaagggtatt ttgatcgatg ggtgttaaat caggtcaatg
     9001 cgtttcttgc tgataattat cacgcagatt ctcctgctga ggcattttgg ggagaccagt
     9061 ctgacgataa gtatcaatgg cagcacattc tggaagagca cctaaaaagc gcccgaagtc
     9121 atttttttaa taatgatttc gatcagtatc tcagacagct acagattaag agtcttgcag
     9181 cgacgtgtca tcaactagtg catttagatc ttcaaccaaa gcatatcttg ttgaacgata
     9241 gtgagattgc gattgttgat tgggaatcga gcagtacatt cggcgatcct gcctttgatt
     9301 tggggaaact actcggtcat tttttgtttt atgcaattca ggcacaacag acagaatcaa
     9361 tcgtgtgtct gatagaggaa gtcgtattga cgtttcagtc acgcacaatc ggtacttgcg
     9421 atcatgattt tatttcgaga gtctatggtt ttattgggag tacattgctt tcattaggcc
     9481 agaatataaa agaaagccaa tttaaacagt tgacagcgtg tgttagaaaa atcgaattta
     9541 cttactattc aaattagaaa atctccagaa gagttgtgct ggtttaagtg aaaatttcaa
     9601 atcatgcagg ggtatagtct tcgtgggtag ccaaatttag agcatgttgg gttgacaaat
     9661 aatttgacaa ctctttaccc aattctgggt gtttgagtgc aagcgcaatg gttgctttga
     9721 ggtagcctaa tttgcttccg cagtcatggc gggtacctgc aaattgataa gcaagaacac
     9781 tttcttctag gcgtaaagat gcgattgcat ctgttagttg gatttctcct atggcgccag
     9841 gcgttatctt ctccaagtgc ctgaagatcc tggggttaag tatataccgc ccaacaatgc
     9901 ccagagttga aggcgcgtta tggggttcgg gtttctcaat catattggta attttatgga
     9961 gatttttcgg acccaaatca cattctacaa tgccatattc attagtttta tgtggatcta
    10021 cgttttgtat tccgagaatt gagtaaggat tattggcata aatttctacc atttgttgca
    10081 taactggttt ttctgcatcg attagatcat cagcaagtag aacagcaaat ggttcattac
    10141 aaatgatcgg gcgcgcacat aagatggcat ggcccagtcc taacgcttcc gcttgtcgga
    10201 tatagataca gctaatatgt ttgggcaaga tactgcgcaa ttcggctagg gtttcctgtt
    10261 taccccttaa ctcaagtgct gactcaagtt cataggcttt atcaaagtga tcttcgattg
    10321 aacgcttatt tcgtcctgtg atgaagatca tttcggtaat tcccgcagat acagcttctt
    10381 caaccgcata ttgaattaat ggtttatcga taaccggcat catttccttg ggactcgctt
    10441 tagtcgctgg tagaaaacgt gtgcccatgc ctgcaactgg aaagatagct ttagttattt
    10501 tcttgatcat gacggtctcc tgctttgggt gattgagata aatgactcat ttgatttact
    10561 cgtttgacta gtcattctag ttttgatggg tgtacccata atatttcctg aaccaatcga
    10621 tgaattgctt aattccatca gatagcgaag ttttcggctc aaaaccgact gcttttttta
    10681 aatctgccac agaagcatag gtagcttcaa cgtctcctgg ttgcatgggt aataaatgct
    10741 tgatcgcttt tttaccgatc actgtttcga gttctccaat aaaatcatct aatagaatag
    10801 gtgtatggct accgatgtta tatatcgcat aaggagcgtc gctacgagct gggttgggat
    10861 tgattcggga atatagcgga tcagctgtgg ctggtttctt taggatgcgt attacgccct
    10921 ctacaatgtc gtcgatgtaa gtaaaatcgc gctgcatttt cccatgattg aatacatcaa
    10981 taggcttctc ttgaaaaatt gcatgagcaa acagacttgg tgacatatct ggcctacccc
    11041 atgggccata aacagtaaag aatcgaagtc ccgtagtggg aattccaaat aaatgactat
    11101 aggtgtgagc cattaattca ttcgattttt tagtggcagc atacaggcta gctggatgat
    11161 caacgttatc ggtgacgcta aatggcattt tagtgttaat accataaaca ctggagctgc
    11221 tcgcatacac aagatgactg actggatgat ggcgacaacc ttccaggata ttcatgaatc
    11281 ccattatatt gctgttaata taagcatagg gattcttgag tgaataacga accccagcct
    11341 gggcagcgag gttgatgact agctgaaatt tttcttcatt aaaaattttc tccattgcga
    11401 ttttgtctgc gatatcaagc gcgatgaagc ggaaattttt aaaagcttgt agttgcttaa
    11461 ggcgatcttc cttgagcttg atatcataat aatcattaag attatcaaga ccgatcacat
    11521 cgaattgatc ttctaagagt tttttggtga catgcatacc gataaatcca gcagcacccg
    11581 tgacaagtgt tttcatcgtt ttaagggcta atttctaccg atcgaatagt attcgattcc
    11641 ttcaacacgg accgcctctg ggtcgtacat atttctccca tcgaaaatga tgggttgttt
    11701 gagtttgttt ttgatcaatg agaaatctgg tgtacggaat gctttccatt ctgtaacaat
    11761 cgcgagggca tcggcatttt ctagtgccga atacggtgta tcggcgagtt gtaaacgtgg
    11821 attgttacca aaaattcgtt tagcttgctc catggccgca ggatcatatg caactacagt
    11881 cgcgttatgt gaccacaacc cttctatcaa aatacgactt ggtgcttctc gcatatcatc
    11941 tgtattcggt ttaaatgcga gtccccaaat tgcaattctt ttaccattta ggttgccatt
    12001 aaattttgta acgatctttt ctaacaaaac ctttttttga cggttgttgg ttaattcaac
    12061 tgccccaaga attttcaatt cgtgatcttg ctctttagcg atatgttgta atgcttgtac
    12121 atccttgggg aagcaggatc caccataacc acaacccgca taaagaaaat gatgtccaat
    12181 gcgtgaatca gaaccgattc catgacgaac atgctcaata tctgcaccta atttttctgc
    12241 aagtaatgct aactcgttca tgaaggatat gcgtgtcgcc agcattgcat tggcagcata
    12301 tttggttaat tctgcggatg ctgtatccat tagaactagg cgattgtgat tatgattaaa
    12361 gggtgcatac aggattttca tcagaccaat ggctttgcga tcatctgcgc caactacaat
    12421 gcgctctggt ttcataaaat cttctaccgc attcccttct tttaaaaatt cgggattcga
    12481 aacaacactg caatcaatta cttttccttg gcgataaagc gtttccgtaa tcactgcttg
    12541 taccttttta gcggttccta cagggacagt ggatttgtct atgattaatt tatattcttg
    12601 catgtgttct gcaatagcgc gtgctacagc catgacatgc tgtagatcag ctgatccatc
    12661 ttcatcgggc ggtgtcccaa cagcgataaa ttgcgctaaa ccgtgttgag tgcttttttt
    12721 tatgtcggtt gtgaaggaaa gacgcccata agcttgattt ctttctacca ggctttcaag
    12781 tcctggctca tagataggta tttttccttc ttgaagcagg gcaatttttt tttcatcgat
    12841 atcaacacac atcacatcat tgcccatctc agctaggcaa gcgcctgtaa ctaatccaac
    12901 atagcctgta ccgatcacgc tgattttcat tttcgtccct tattgtttgt gttaattact
    12961 ttgaattacg aaggttgtta aatatcgaaa tacgctaaaa aaagaactgc acatccaaac
    13021 gtgtgaagaa tgctgatcct ctctgttcgc cgtaggctgc tcctggtaaa aagtagccag
    13081 ttctggaacg taaccgaaca ttgttgatgc cacgatagcc gagaaccaaa tcaagttcgt
    13141 caccgagatg ggaatgaata ccatccggat ttcgactgaa tgtgataggc ccatctcgaa
    13201 ggtctcgcgg taatcttgca aaagcatggt cttgagcata gcggtgataa actagttcga
    13261 tagaagaacg ttcggttggg cgaattccca gggcagcagt caaaatgtgc atattccgga
    13321 gctcggggtt atataaaact ccgtagtaat caaagttgac aacgccacca aatcttccgc
    13381 tattggcctg aaagcctgtc tggcggaaat tatgatcagt atcattttct ggattgctat
    13441 caccggatcc gtacgcatag ccaaaggtga cataaggttt tgcctgaaat tttcgagcaa
    13501 ttgcagtaac accaagatca attcctaatc cactgatctt ttttgaatca gcgctacctg
    13561 aaacgaaagc agcatccaac cagtattgaa ttctgggttt taataaattt gaaagaaatt
    13621 ctgattcact ggatgcgagc ttaaactttt catcaccaat gaatcttatg ccgctccaca
    13681 ttggattttc atttctaagc aacccaggtt cgattctgtc ctgatgcttg ttacctaaga
    13741 ttagatatcc gcttatgaaa gatctttctg ttaaggcata atcaagattg aaaagaatat
    13801 ccatctgttc tttcttgatt gcaatattgt caaaaacttg taatgagtgg ttggtaacca
    13861 aaactggtgt ggagatggct gtaaataaat cgaatggccc acttttagct gataaacgtg
    13921 cggcatccat tcgatcagta aaataccacc gccgtaaatc aaaaaactgt tgtctaccta
    13981 aagttaaatc aattgggatg gtttccaaaa tgggcgctgt cagaaaaaac tcacgtactt
    14041 gcagttcgaa gaaattgctt ggttgagtat cgtcttgtag ttttatgcta tcccgtagac
    14101 gaaattcagt gaataaagaa tagccttttg gaaaatcaaa aattgtattg agatcaacta
    14161 atggtctaaa tctaaattga tctactgcat cattttcccg aaatgagaag ttgttttcga
    14221 aagaaaaatc aagcagtgtg gcagcgcctg tgaaactaat gtctgtattt ccgtattgaa
    14281 agtgccttgt tgctttaggt cgcaatcgct catcatctcc tccaagcccc attgctcttg
    14341 aatcaacatc gtcaatgacg gtgttttgcc ttccccgcct gatttgatca ttagagaaag
    14401 actcacaact gattgacata atagttatac atagaatttg acaaaccaaa catgtcgcag
    14461 atatgtgaaa acggtctata tatcttctta atgttttggt attcatgagt ttgcatgggc
    14521 gatattttgt ccggtcgtaa gaagatcgat ttgtaaggtc ctagcaattt aattgaaatg
    14581 gctaaatata gatttcatac cgcatggctc tttttattga gtttgaaact cataaacgag
    14641 tgattatttc cctcgatcta aatttaatat acgtgggtta atttcccgtg tcaaataaat
    14701 ttagatgagt aactgcaaat caaatgagag aaaatttgga agcgctaaac taaatagagt
    14761 caggcgctag taagcctgat tgttggtcgg tagaaaaaat tgaatgttat cgataaataa
    14821 aaatatccga ttccttaggc gctctttatg gccatgccca aggagtaaga tagtgcttgt
    14881 ttactttttc actagccttc ggtgtatatc agtggtaatt acctcaacct gaataaaagg
    14941 aacaaaataa ctcttgcttc ttaataactt cctgaactcc tcagaagctg ttcacagttc
    15001 tttttttaac caatagcatc aaataatatt tattgttgca gtacgctcgg taatagaaat
    15061 aagcgtttac gatgcgcttg ttgcgtatga cgctttgggt aagcttgcac aagttttatc
    15121 tagcgctaat aaataaaata aaactgaaca tataagccgg agtaaatttt tattacatca
    15181 ataaaaaaat agttgacgtg taaaattttc acacatataa tttttctcat gcgtgaaaaa
    15241 attccatgca tttcggtagg taatttaata atgcttcatt tctaattaat taactttagg
    15301 agaaaaacat gagctcacgt gatgatgaca atagcagcgg tcgtagcagt gatgatggga
    15361 tcaatcatga tatcaatgac gatcatggta gaggacgtgg tagtgatgat ggcgttaacc
    15421 atgatattaa cgatgatcat ggcggacggc gcaatgatga cagaagcagc gatgacaact
    15481 cttctggggg tatcaatgtt tctggaaatc ataaagcttt caagtttgaa attgtaaatg
    15541 gccaagtaac cgcagttttt gaagtgaaag acggggaatt aaaatctaaa tcactaacgg
    15601 atggtggaag aaagagctat accgtcgatg gtaatgatgt tattcgtact gaaattaaac
    15661 catttggaac agagattacc cggtattcag acgataatgg ggacggaatc tatttacgta
    15721 cttctgagca atggcaggtt tcttctaatt ccaatggagt cacgcctaaa tttactgaca
    15781 tcattcattt ttcgcatacc agcggtgatg atcgtattgc ggtgagaggt ggggaagatt
    15841 gtcgcggtgg gaatggagcg gatgatttcg ttgtgcgtga agcagcacat ttacatatcg
    15901 gtgattttag ctcacgtgaa ggtgatttgc ttatttttga tacgggatta ggattaacat
    15961 ccagagatca gttagcgagc tatgttactg atacgcatca tgatggtcaa aatttcatcg
    16021 ttaattttgg ctcagatgtg tccattacct tagtaggtgt tcaggctgac caagttagct
    16081 ggaatgatgt gagtgtttta agttaaacat cgtaaatggc gattgtgagg ggataataat
    16141 ttcttcacaa tcagtaaatc aaaatagtaa ggggggattt ttgatgctat atcgactcaa
    16201 taaagcgatg caatccatgg cttatggcat agtaagtttg ttctgtttgg tactatcgac
    16261 aggaagcgtt ctagcaaagg ataacgaagt cagttttgca ttaactgaca atgctgggcg
    16321 ttggttcgat accggcactg caattgcggg aaatcgttca attgccgttg caacacccgg
    16381 tgtccgtgtt aatttttctg gaaactcaaa tacggttcat acaaggacca gtttgatcta
    16441 ccccactggc gcagcgggca tgccatttaa taccgagcct aagaagggtg gggatagtat
    16501 tactttgaca acccctggcc tctatgtgtt cacgtgcagc attcacccgt atatgtttgg
    16561 tgcagtgatc gtagatgatc cagccacaag cgggcttgat ttaggcgctt cgattagctt
    16621 gatcaatggc attacagtac cgaccagtag cgatctggca acgcgattac tgcgtacttt
    16681 ctttattgca accaatccag caaactggca aaactatgca agcaatgcgc cttggcatat
    16741 tacttacccc aatgtggatg tacgtgtcga tattggagtt gtgaacctac ctgcagtact
    16801 caatgcgcgc tatgggaatg atattagtct gccagcctta agtaatcctg ctacgcaagc
    16861 tgtcggtgaa gtctgggtgg caacgcaatt tgaaacgaca tctgggaaat ctaagcccgg
    16921 aactgtgacc gcagtcgatg gttcttcctg gcaagtaacc cgtaaagtcg cattaccgtc
    16981 gatcaatatg aataatccac ataatatgtg gactgacaaa gatcaaaatg tgatctacgt
    17041 tacacaatgg tttgataaaa aattgaccgt ttataaccgc caaactggtg cgctgatcag
    17101 gaatgtttca gtgggcgagg cacccgccca tgttatgaca ctgaccgata atgatcggat
    17161 tcatgtcacc aataatgggg atacgcgaac cgattcagtg atggaattgt ctccgttagc
    17221 aactaatgtg ttgcgccgaa ttgatattgg gcgtggcaat cctcacgcac attggatgag
    17281 ccatgacggt aagttcatgg taacgcctaa tgtgttaaca ggagatacat ctcaatataa
    17341 ttttaggact aatgatgtcg aagcgattct gccagtcagt ggaccgctaa gccatccttt
    17401 agcgactggt atgatgccgg atgcgagcaa atattatgtg gccaacttac tcgatagcac
    17461 gatcacggtc gttgatatgg aatctcacaa tgtgttgaaa catattaatt taatcgcaaa
    17521 ctaccatccg gttacgggtg cgataacagg gcccgtaggt gctttaccga tccaaactcc
    17581 ggtttcaccc aatgggaaaa atatggtgac cgctaataca ttgaccggga cgattaccat
    17641 tctcgatact gcaacagatg aaatcgtagc gatgttgccc tgtgatccag gctgtcacgg
    17701 tgtgcaatat ggtgctaagc tcgggggcgg ttattatgca tatgtaacca gtaaattctc
    17761 gaatcgcatg ctagtggttg accctgatcc taataatgat ggaaatccgg cagatgcggc
    17821 tattgtgggg agtatcggac tttttgctac gaatcgtacg ttaaaagatg ccaccatttc
    17881 tggtaatgcc ggaatgggtg ggcaagggat tcttcctatt cccgttgtgt acaatggttg
    17941 ggtacaaaaa ctaccttcta actggagtaa tctgttaaca cctgcgcagc gcaatccatg
    18001 gtaactacta cttttaaacg ttagcgaaga ttatgctgtc agttaatatt tgaatctgcc
    18061 atttaacctt gcggctagat taaaaactta taagttacaa aataaaacca agctgatatg
    18121 gacgttcagc ttggtttttg ttttttctag gaattgattt cgatagatca gtcaggaatg
    18181 tagttatgct tcctaaacac tatgctggaa agctgtgcag aatgttaatc gatagcaaaa
    18241 cgttgaatta catctacgat tcatttagca tgatagaatc ctgctatact taaaac
//
LOCUS       DAOQKQ010000031        17836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_1424_length_17836_cov_5.671616, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000031 DAOQKQ010000000
VERSION     DAOQKQ010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 17836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17836
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1424_length_17836_cov_5.671616"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="PKZ49_03345"
     CDS             complement(<1..980)
                     /locus_tag="PKZ49_03345"
                     /inference="COORDINATES: protein motif:HMM:NF013124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HND35627.1"
                     /translation="MDQSNRNIQLGQKSVYAEQGDVYVTYAGERRIPHALTPPPFLTE
                     IFLGRQDDLQLIHDKLFASGSNLLLVNGEGGIGKTTIASKYYHTYQHEYIHVAWVLSE
                     KSIANALLLLAVPLNLQFDERMNTDERIGLLLAALMNLEKPCLLVIDNANELSDLEAN
                     YQRLRRSSNFHLLLTTRITHFERAEACAINGLPQDDALALFKKYYRVLDDGEEALFFQ
                     IREAVGGNTLVLELLAKNLVGQNRLKPRYSLSNLLADLQSQGLLQLSHSQAVGTDYQS
                     KGAMRRETPEAIISAMYDLSGLPAQEGALLSVFAVLPAESIPFARLETLLP"
     gene            complement(992..1570)
                     /locus_tag="PKZ49_03350"
     CDS             complement(992..1570)
                     /locus_tag="PKZ49_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35628.1"
                     /translation="MKKIKIFLASSKELQSEREHFEREIYRKCKVWFDRGIFLHLDIW
                     EDLSARMSLEGSQSVYNQYVKAADLFVLLVHSKVGMYTAEEFDIAFGQFQSTKKPFIF
                     TYFKTPNGVATDASLAEFHHKLKDLHHFYSPFNDENDLWVQFNKELDRLETVEFTEFK
                     HENMHQTGRSITQGDNSVYIEKAGDGTTINIQ"
     gene            complement(1642..1851)
                     /locus_tag="PKZ49_03355"
     CDS             complement(1642..1851)
                     /locus_tag="PKZ49_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35629.1"
                     /translation="MNFGAPHDSVVWLIFLIVRWLEEIYDHVRGLLPVYIVKAGFDRL
                     EEVACYRTIAGVVILYHASTSIHQK"
     gene            2064..2984
                     /locus_tag="PKZ49_03360"
     CDS             2064..2984
                     /locus_tag="PKZ49_03360"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HND35630.1"
                     /translation="MSMADRDGVIWYDGKLVPWRNATTHVLTHTLHYGMGVFEGLRAY
                     ETDQGPAIFRLPEHTERLFHSAHIFKMPIPFDPATLMQAQKDVVRENNLKSGYIRPII
                     FYGPEAMGLSAKNLSVHVAIAAWSWGTYLGPDSLLKGIRVKTSSFTRHHVNINMCRAK
                     SVSTYTNSILAHQEVALDGYQEALLLDVDGYVAEGSGENIFVIKNNKLYTPDLTACLE
                     GITRASVIELAGEIGLPIIEKRITRDEVYCADEAFFTGTAAEVTPIRELDNRTIGNGS
                     RGPITERLQTLFFECVSGKSKKHTDWLHYV"
     gene            3009..3194
                     /locus_tag="PKZ49_03365"
     CDS             3009..3194
                     /locus_tag="PKZ49_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HND35631.1"
                     /translation="MNNRQIEVTVDDLPLHCPMPSMLSWNGHPRVYLPIEATREALCP
                     YCGTHYVLKGEVKTNHH"
     gene            3307..4683
                     /locus_tag="PKZ49_03370"
     CDS             3307..4683
                     /locus_tag="PKZ49_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HND35632.1"
                     /translation="MNDIPKEIFKAYDIRGIVNTVLTPDMVERIGHAIGSEAVKRKLS
                     SIAIGRDGRLSSPSLAQALAKGLQKSGLHVIDVGMVPTPVLYYAAHELCGYTGVMITG
                     SHNPPEYNGLKMVLGGETLAAETIQSLRKRLEENDLTHGEGSYKTHEITSEYLQRITS
                     DVKLSRPMKIVVDCGNGVTGAMAPALYRQMGCEVIELFCEIDGNFPNHHPDPSVPENL
                     QNVIETLAATDAEIGFAFDGDGDRLGLVTKDGSIIFPDRQLMLFAADVLTRNPGAKII
                     FDVKCTRNLALWIEQHGGIPIMWKTGHSFIKGKLKETGALLAGEMSGHIFFKERWYGF
                     DDALYTGARLLEFLSKQSDMNAVLHALPDAINTPELQIKLKEGENYALVDLLQREARF
                     DNPERIITTDGLRVEYRDGFGLIRSSNTTPVLVLRFEADNSDALQRIKLDFRRVLKQF
                     KPDISLPF"
     gene            4750..6267
                     /locus_tag="PKZ49_03375"
     CDS             4750..6267
                     /locus_tag="PKZ49_03375"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HND35633.1"
                     /translation="MKKYFHFIFILALLLPISTIADDLPELGDVSQATITPRQERQLG
                     YQIMRQIRADPSYLDDPEITSYLNNLGRRLLSTSNETQPGQSFEFFALQDPTINAFAL
                     PGGFMGFNSGLIIAAQSESELAGVMAHEIAHVTQKHLARMIAGQQHMGLISTIAGLAI
                     AILASRSNPQAGSAAIIASQAAAIQSRLNFTRKHEKEADRIGFNILHRAGFDPHGMSA
                     FFERLQLAGRYRENGAPSYLRTHPLTYERIADIESRMRELPYRQIPDSIDFHLVRAKL
                     KAALGNSTDTVAQFDSRIHDKRYSNELAERYGLIYALLRDKRFKRAEKELESLYQKMQ
                     SDPTIKNLRNHPLGDLILIESDYLQSGAMIDTLAARVKIANKQIPDAFKLYQAALKTY
                     PQHRALIYDYAAALLSHQGPQAALNFINKQSQVIHDDIHLYRLEAQCHSALGNHMLKH
                     RAQAEIYIQEGKLHAAVEQLEIALRYSNGNFYQLSSVEARLRQLKEFIASFEEES"
     gene            6425..7519
                     /locus_tag="PKZ49_03380"
     CDS             6425..7519
                     /locus_tag="PKZ49_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HND35634.1"
                     /translation="MIYGYTRNHQPSAKEIYKTRLVDRGDVIQVISANGTLTPLELVN
                     VGTQVSGTVAKIYVDFNDQVKKGQILAELDQALLRAQLLQSEANYQSAQATLNITQNK
                     ALRGRTLLQKGFISKEALDDLEQQLVLAKAQVAISKATVERDRANLNYSVIRSPISGV
                     VVARDINVGQTVAANFQTPILFQIAKDLYQMQINISVAEADIGQIQTTQEITFNVDAF
                     QEREFTASVKQIRLNPTIQENVVTYNVVAAVENRDGILLPGMTANVRFVINHKRDVLR
                     ISNAALRYKPNRDEAQISGVNQAPQGTQLYRLKNQVQQPVQVTTGVTDGHMTEIVSGD
                     LNVGDEIIVSDNKSSKSKESSASNFRFRMF"
     gene            7519..8256
                     /locus_tag="PKZ49_03385"
     CDS             7519..8256
                     /locus_tag="PKZ49_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HND35635.1"
                     /translation="MSVVNQPAPNPILIQIDRLCKSYELTDRSGELISQPVLFDISLT
                     INQGEFIAIMGHSGSGKSTLMNILGCLDTPTSGNYWLSGRNVATMNSNELAHVRNQNI
                     GFVFQSFNLLKRMTALENIAIPLLYAGIGRKESLKRSQAMLDLIGLGKYRDHLPNQLS
                     GGQQQRVAISRALINQPQLLLADEPTGNLDTQTSHDIMTLFTQLNRNQGITIVLITHE
                     EDIAAHAQRLIRLKDGRIVQNEIKLSN"
     gene            8258..9481
                     /locus_tag="PKZ49_03390"
     CDS             8258..9481
                     /locus_tag="PKZ49_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HND35636.1"
                     /translation="MNLLVIIGEALRALKLNRLRTGLTMLGMIIGVAAVVLMLSIGQG
                     AQININNTIASMGSHLLIVVPGATSSGGLRFGSGSVRTLTISDAQAVAELPSIEATAP
                     VISGIAQLSYSANNWSTSITGVTPDYFSVSNWELADGSIFADADLRSAIRVAVLGSVT
                     AENLFGNEDPIGKTFRISNKPFLVVGILAVKGQSLSGRDQDDNVFVPITTAQRQILGN
                     QFPGSINHMLVQAKSSDTLDEAEAEITQLLRQRHRIADKMENDFTVRNLTALATVAST
                     TAKVMAWMLGSIASISLLVGGIGIMNIMLVSVTERTREIGIRMAIGASYRIILMQFLL
                     EALVICILGGLIGVALGIGGAWMVSQSTDMPIVVTFNMIGLAFIFVTAIGIFFGFYPA
                     RKAAALKPVDALRYE"
     gene            complement(9587..9724)
                     /locus_tag="PKZ49_03395"
     CDS             complement(9587..9724)
                     /locus_tag="PKZ49_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35637.1"
                     /translation="MRFALFAAVLAAFMLTACGDPKPGQYPPSIMMERDSMPDKGEDK
                     K"
     gene            10008..10778
                     /locus_tag="PKZ49_03400"
     CDS             10008..10778
                     /locus_tag="PKZ49_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HND35638.1"
                     /translation="MIISSVLIAFRNLFNLKIQWILIWPLIVSCLIWILLSHFLWAHV
                     SGGIVEFISTTQIKDWLEESYLVKIANSLEGLVNILLFIALVIITTLMITALFVMPSL
                     INFVAKQYYPHLERKRGGSVMGNVKHVLSALLVFLLLWSIALPLWFFGIGIVISFIAA
                     AYFNQRLFCYDALSEHASPEELTQLLASNKPSLWSLGLLTGFLQFVPFLNLFAPTWTA
                     LAFVHYELGRLDKLRASTKQENPIANLSLNHKQSVDEE"
     gene            complement(10786..11337)
                     /locus_tag="PKZ49_03405"
     CDS             complement(10786..11337)
                     /locus_tag="PKZ49_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HND35639.1"
                     /translation="MNVLNSTEIEQPSLLIVDDDSVFCEVLTKAMVKRGFEVICAHTI
                     ASAMEKIESLVPEYAIIDLKLADESGLTLVEKIRKLDPGTRIIMLTGYASIATAIEAI
                     KLGATHYLAKPVDVNEILAAFERTSGEANVPISPNPLSVDRLEWEYIHRILAENNNNI
                     SVTARILNMHRRTLQRKLAKKPV"
     gene            complement(11365..12672)
                     /locus_tag="PKZ49_03410"
     CDS             complement(11365..12672)
                     /locus_tag="PKZ49_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HND35640.1"
                     /translation="MKSNARASHMFNRLLLSPLNKNLHRLFLLRSIAIILQCPLFFLV
                     YSFIEQDIPWAELIAVVVGLAIINMMTWYRLHQNWPVTNLEFFLQLLVDVLALTALLY
                     FSGGSTNPFISFYLLPLIIAATILPWRYTWLIAAISIACYTILLFEYVPLPHDHSDDH
                     SAHLMEFNLHVSGMWFTFVLSTVLIAWFIVRMSHSIRQRDKELAEAREQALCNEQIIA
                     LGTLAAGAAHELGTPLSTMAVIAGELQQEFPENGEYQKNIYTLRDQITHCKHILTQLL
                     ANAGQARTENASTQSVDVFLKQVLDKWTLIRPSVKFQYSCDGQQPVPHIMNTQLLSQS
                     ILNLLNNAADASVNQVKIRSYWDQNSLNLEIHDDGEGLTPEVMQHAGEAFFTSKAPGQ
                     GFGIGLFLANANIERFGGSVHLFNHPEGGACIHLTLPLLPQSS"
     gene            complement(12811..14184)
                     /gene="mpl"
                     /locus_tag="PKZ49_03415"
     CDS             complement(12811..14184)
                     /gene="mpl"
                     /locus_tag="PKZ49_03415"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HND35641.1"
                     /translation="MHIHILGICGTFMGGLAVLARESGHKVTGCDLNVYPPMSTQLRS
                     LGIELIEGYSPEQLKLNPDLYVIGNVVTRGNPLMEAILNQNLPYSSGPQWLAEHVLRK
                     RWVLAVAGTHGKTTTSSMLAWILSFAGLDPGFLIGGIPENFGVSARLTGESVSGTNRE
                     ASPFFVIEADEYDTAFFDKRSKFVHYHPRTLILNNLEYDHADIFPDLASIQKQFHHLI
                     RTVPSNGLIVANGRESSLQQVIAQGCWTPIEFFGVPTEWAVKLSSQGAIEVYFNEACQ
                     GTLDWDLLGEHNLMNALAAIAAARHAGVPSQMGLLALSQFKNVKRRMEVKGIVNNITV
                     YDDFAHHPTAIRTTLEGLRTKVGEARIIAVLEPRSNTMKMGVWKDSLAQSLNIADQIF
                     CYTDQVDWDVTSALQSIKHKAVTYSQMQELINAVALMAHAGDHILIMSNGGFGGIHEK
                     LLLMLRK"
     gene            14346..15287
                     /gene="gluQRS"
                     /locus_tag="PKZ49_03420"
     CDS             14346..15287
                     /gene="gluQRS"
                     /locus_tag="PKZ49_03420"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HND35642.1"
                     /translation="MRQIQETTKYRGRFAPTPSGMLHFGSLVTAVGSYLDARSHQGEW
                     LVRIEDVDHPRVKAGISEKILSTLEQFGMEWDGPVIYQSKCMNRYQDALHFLGSLGLT
                     YPCSCTRKEIADSALLGLEGMVYPGHCRNGLPPDHNQRPQAVRIKTKPDRIEFQDRLQ
                     GSVYQSIANEIGDFVLRRADGIYTYQLAVVADDGWQSITHIVRGADLLASTPRQIFLQ
                     RLLGYPTPSYLHLPVVTTAQGEKLSKQTLAHPIDTENPLLQLMAAIDFLGQHPPSELI
                     EGDLNSFWQWAINHWSVTRIAQVTYAPYNPSLNIVNE"
     gene            15369..16526
                     /locus_tag="PKZ49_03425"
     CDS             15369..16526
                     /locus_tag="PKZ49_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HND35643.1"
                     /translation="MAVLPFYISNKKNLKIVIVGGGYAGIAALTTIARYQPSAEITLI
                     DPKIDHIKITHLHETFRYPLTDFLIPFTDIEQEFGCRHLPLELPFSESTLLQWERDKT
                     ISINNESIPFDYLLIASGALNNNADQDSGDVMGLNAFMKHPGSEILSQFLSKPGTEAP
                     ISVIGGGATGIQFLFEIEHFLRYHKYKNKLRLIDSGDQVLKQFPDRFARYVEAAMKNL
                     NIEYYPTTYFRGQQHSQIRLEDKKTSHQFELPSSLTFLFSGKSQGSVLKANAFGQVIV
                     DQQILQHIFVAGDCAYYQSIGSNALTAQSAVRKGKLAARNLLRHSGLLKILEPYLHRD
                     IGYVVSLGPADAIGWLALENNVVTGLAAYTIKEIVEAQYDLLLAGIDTYLL"
     gene            16551..17021
                     /locus_tag="PKZ49_03430"
     CDS             16551..17021
                     /locus_tag="PKZ49_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide cyclase"
                     /protein_id="HND35644.1"
                     /translation="MFNLFDDKPVAGKASTVIQSPDDKLFNFIGHELLTNYPRWSPEV
                     KELEKLTDSPLGLGTLCRQVRVDQGNRSESTFKIAVFEPNKQICFNGVSNPFRCDYSL
                     ESKNQESTQITFTFELLSLELHMRPFEKLIRIAVQDGAERTVRNIKRLIEFNTI"
     gene            17087..17539
                     /locus_tag="PKZ49_03435"
     CDS             17087..17539
                     /locus_tag="PKZ49_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HND35645.1"
                     /translation="MDFIVEKDPGLIPQVLSNILDSCVNGITLADPDQEDLPLVYANK
                     AFETITGYTQAETIGKNCRFLQGNDRDQQERHQLREAVLNKQSIEITLRNYRKNGEMF
                     YNHLVITPLFDSKGNVLYYLGVQFDVTQQKKAEEEIKRLTEQLAKLSK"
     gene            17658..17745
                     /locus_tag="PKZ49_03440"
     tRNA            17658..17745
                     /locus_tag="PKZ49_03440"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17692..17694,aa:Ser,seq:tga)
BASE COUNT         5217 a         3883 c         3750 g         4986 t
ORIGIN      
        1 ggcagcagtg tttcaagcct tgcgaaagga atactttccg ccggcagcac ggcaaaaacc
       61 gagagcagcg cgccttcttg cgcaggcaat ccgctgagat cgtacatggc agagataatg
      121 gcttccgggg tttcccggcg cattgcaccc ttgctctggt aatcagtgcc cacggcttgc
      181 gaatggctga gttgcagcaa accctgactt tgcaggtcgg cgagcaggtt ggacagacta
      241 tatcgtggct tgaggcggtt ctgcccgacc aggtttttag ccagcagttc gagcaccagc
      301 gtattgccac ccactgcctc gcggatttgg aagaataatg cttcttcgcc atcgtcgagg
      361 actcgatagt attttttaaa cagtgctaga gcatcatcct ggggtagtcc gttgatggca
      421 caggcttcag ctcgttcaaa atgggtgatg cgcgtggtga gcagcaggtg gaaattgctg
      481 gaacggcgca accgctggta attcgcttcc agatcagaca attcgttggc gttgtctatt
      541 accagcaggc agggcttttc aagattcatc aatgctgcca gtaaaagccc aatgcgttcg
      601 tcagtattca ttctttcatc aaactgcaaa ttgagcggca cggcaagcag caacaaagcg
      661 ttggcaatgc tcttttcgct cagtacccag gcaacatgaa tgtattcatg ctggtaagtg
      721 tggtaatact tggaggcgat ggtggtttta cctatgccgc cctcgccgtt cacaagcaac
      781 aagttgctac cggaagcgaa caatttgtca tgaataagct gcagatcatc ctggcgccca
      841 aggaagattt cggtgagaaa aggcggtggg gtgagggcat gagggatgcg gcgttctccc
      901 gcataggtta catacacatc cccttgttcg gcatagacgg atttttggcc tagctgaatg
      961 ttccgattgc tttgatccat gatggtagcg gttattgaat gttaatcgtc gtgccgtcgc
     1021 cagctttttc aatataaaca ctattgtcgc cttgcgtaat gctacggcca gtttggtgca
     1081 tattctcgtg tttgaattcc gtaaattcaa ctgtttccag tctgtccagt tccttgttga
     1141 actgcaccca aaggtcattt tcatcgttga aagggctgta gaaatgatgc aagtctttga
     1201 gtttatggtg gaactcagca agacttgcat cagtagccac cccattgggc gttttgaaat
     1261 aagtgaaaat gaatggtttc ttcgtcgatt gaaactgtcc gaaagcgata tcaaactctt
     1321 cggcggtata catgcctacc ttgctatgta ccagcaacac gaacaggtcg gcagccttga
     1381 catactggtt atacacgctc tgcgaacctt caagcgacat gcgggcagac aaatcttccc
     1441 agatatcgag atgcaagaaa attccccggt caaaccaaac cttgcatttg cgatagattt
     1501 ctcgttcgaa atgttcacgc tcggattgga gttctttaga tgaggcgaga aagattttaa
     1561 tttttttcat ttttaatcag gatgatgaat tcgttagttg aaaaaatatc atgcctctat
     1621 ttgcccattt tggggctgtg atcatttctg gtgtatcgat gtactagcat gatataaaat
     1681 gaccacgcct gcaatcgtcc gataacaggc aacttcttct agccgatcaa atcccgcctt
     1741 tactatataa actggtaata aaccacgaac atgatcgtaa atttcttcaa gccaacgcac
     1801 gatcaagaaa atcaaccaca cgacggaatc gtgcggtgct ccgaaattca ctacatgaat
     1861 cgccccgcgt ttgaactgcc ccccagaagt tggacattta caaaatatca ccaaatgggt
     1921 cagcgcactt taacgttaac ccattccggc atacagtgga actaaacagg gagattactc
     1981 aggttaggta cttcaagctt tcattggtta attaaaaaat aagctagaat attatttttg
     2041 gaatttttat cgaggatttt gtaatgtcaa tggctgatcg cgatggtgta atttggtatg
     2101 acggaaagtt ggttccttgg cgaaatgcta caacccatgt gctaacccat acgttgcatt
     2161 atgggatggg tgtgtttgaa gggttgcgtg cttacgaaac cgaccaaggg cctgctattt
     2221 ttcgcttacc ggagcatacg gagcgcttat ttcattcggc acatatcttc aagatgccca
     2281 ttccattcga tccagcaaca ctcatgcaag cgcaaaagga tgttgtacgc gaaaataatc
     2341 ttaaatcggg ctatattcga cctattattt tctacggccc agaagccatg ggactctctg
     2401 ccaaaaatct atcggtacat gtcgcaattg cagcttggtc atggggcact taccttggac
     2461 cggatagtct tttaaaagga attcgcgtca aaacttcatc gtttacccgg catcatgtca
     2521 atattaatat gtgtcgagcc aaatcagttt cgacttatac caattcgatt cttgctcacc
     2581 aagaggtagc actcgatggt tatcaagaag cgcttttact ggatgtcgat ggttatgtgg
     2641 ctgaagggtc cggtgaaaac atctttgtta ttaaaaataa taagctgtat acccccgatc
     2701 tgacggcctg tctcgaagga attacacgtg cttcggttat cgaattggcg ggggaaattg
     2761 gccttcctat catcgagaag cgaattacca gagacgaagt ttactgtgcc gatgaagcct
     2821 tctttacagg aacggctgca gaagtaacac cgatcaggga gctagataat cgaacgatcg
     2881 gaaatggctc gagaggtccc ataactgagc ggttacaaac tttatttttc gaatgtgtca
     2941 gtggcaaatc aaaaaaacat actgattggc tacattatgt ctgattttat taaggggaat
     3001 ctattacgat gaataaccga caaattgaag taaccgtaga tgacttgccg ctgcactgcc
     3061 caatgccctc catgttgtcc tggaatggcc atccccgtgt ctatttaccc attgaagcga
     3121 ctcgcgaggc cttatgtcct tactgcggca ctcactatgt tttaaaagga gaggttaaaa
     3181 caaatcatca ctgaaataat ggtattgggt gtaaagcaca ttcaatacag gaaagaaaat
     3241 cattaatcct atcttttctg ctatgcatac cactcacatc taacttaatc gataaagagc
     3301 gctgctatga atgacatccc taaagaaata ttcaaagctt atgatattag aggcatcgtc
     3361 aacaccgttt tgacccccga catggtagag cgaattggcc atgccatagg gtcagaggcg
     3421 gttaaacgta aactctcctc aattgccatt ggtcgtgatg gaagactatc cagcccttcc
     3481 cttgcgcaag cgctggccaa agggctacaa aaaagtgggc tacatgtcat cgatgtcggc
     3541 atggttccaa cccctgtgct ctattatgca gcgcatgagt tatgcggtta tacgggggta
     3601 atgatcacgg gcagccataa tcctcctgag tataacggtc tcaaaatggt attaggtggc
     3661 gaaactttag cagcagaaac cattcaatcc ttacgcaaac gcttagagga aaatgatcta
     3721 actcatggtg aaggtagcta taaaacccat gaaatcacat cagaatacct ccaaagaatt
     3781 accagtgatg tgaaattatc gcgcccgatg aaaatcgtcg ttgattgtgg taatggtgta
     3841 accggtgcga tggcgcccgc gctttatcga caaatgggtt gtgaagtcat cgaattgttt
     3901 tgtgaaattg atggtaattt tccgaatcat catcctgacc catccgttcc tgaaaatttg
     3961 cagaatgtca ttgaaaccct tgcagcaact gatgcagaaa ttgggtttgc atttgatggc
     4021 gatggggatc gattaggtct cgttaccaaa gatggcagta ttatttttcc tgatcgtcag
     4081 ctcatgttat tcgcggctga tgtattaacc cgcaatccag gggcgaaaat tatttttgat
     4141 gttaaatgta cccgcaacct ggcgctatgg atcgaacaac atggtggtat tcctattatg
     4201 tggaaaaccg ggcattcctt tattaaaggc aaactaaaag aaacaggggc actattagca
     4261 ggtgaaatga gtgggcatat ctttttcaaa gagcgatggt atggatttga tgacgcctta
     4321 tatacgggtg cacgtttatt agaatttttg agtaaacaat cggatatgaa cgcagtgctt
     4381 catgcattac ccgacgcgat caatacaccc gaactacaaa ttaagctcaa agaaggtgaa
     4441 aactatgcct tagtcgattt actgcaacga gaggctcgtt ttgataatcc tgagcgcatc
     4501 atcacaaccg atgggttgcg tgttgagtat cgtgatggat ttggtttgat tcgttcttcc
     4561 aacaccacac ctgttttagt attgcgtttt gaggcagata attctgatgc gctacaacgg
     4621 attaaactcg attttcgtcg agtattaaag caatttaaac cggacatcag tttgcccttt
     4681 taaaaacttg acccaaccag gcaatcgctg tcactggaac tcgaatcgaa agtcatcatc
     4741 caatctggca tgaaaaaata ttttcacttt atttttattt tggcgctgct attgccaatt
     4801 agcacgattg ctgatgattt gccggagctc ggtgatgtct cacaggcaac cattacacct
     4861 cggcaagaaa gacagcttgg ttatcaaatt atgcgccaaa tccgagctga tcctagttat
     4921 ctcgatgacc ctgaaattac cagttactta aacaacctgg gaagaagatt gctatcgact
     4981 tccaatgaaa cacaacccgg ccaatcattt gaattctttg ctttacaaga tcctacgatt
     5041 aatgcgtttg cattacctgg gggattcatg ggttttaata gcgggttaat cattgccgca
     5101 caaagcgaat cagaactggc cggtgttatg gcgcatgaga ttgcccatgt cactcaaaaa
     5161 cacttagcaa gaatgatagc ggggcaacaa cacatggggt tgatatcaac cattgccggt
     5221 ttagctatag caattctagc atctcgctct aatccacaag ccgggtcagc cgctatcata
     5281 gcaagccagg ccgccgccat tcaatcacgc cttaatttta cgcgcaaaca cgaaaaggaa
     5341 gccgatcgca ttggcttcaa tattctccat agagcgggat tcgatcccca tggaatgtca
     5401 gctttttttg aaagattaca attggcgggt cgctaccgcg aaaatggcgc accttcctat
     5461 ttaagaactc accccctgac ttacgagcgc atcgcggata ttgaaagcag gatgcgtgag
     5521 ttaccctatc gtcaaatacc ggatagcatc gactttcatc tagtacgcgc taaattaaag
     5581 gctgccttgg gaaactcgac tgacaccgtt gcgcagtttg attcgcgcat tcacgacaag
     5641 cgctactcaa atgagttagc agaacgttat ggtttaattt atgcgctcct acgagacaag
     5701 cgctttaagc gtgctgaaaa agagctagaa agcctttatc aaaagatgca atctgatccc
     5761 acgataaaaa acctccgcaa tcaccctctt ggagatctta tcttaatcga aagtgattat
     5821 ttacaatcag gggcaatgat tgatacgctt gcagcacgtg taaaaatcgc taataaacaa
     5881 ataccggatg cctttaagct ttatcaagca gcattgaaaa cctatccaca gcaccgggca
     5941 ttaatctatg actacgcagc cgccttattg agtcaccaag gcccacaagc cgccctcaat
     6001 tttatcaata aacaatcaca agttattcac gatgatattc atctttaccg cctagaagcg
     6061 cagtgccatt ctgcgcttgg caaccatatg ctcaagcatc gtgctcaggc agaaatctat
     6121 attcaagaag gcaagctaca tgccgccgtt gaacaacttg agattgcgct tcgctatagc
     6181 aatggtaatt tttatcagtt atcgagtgtt gaagcgcgct taaggcagct taaagaattc
     6241 attgcaagtt ttgaggaaga gagttgatct attgccttga aattgaccca atcatgctaa
     6301 caaggttaca cacacttagt tagcaaatcc ctaatcagca ccaacacttg ttttatctct
     6361 ttccatgata cgtcgcaatt tcagaacttt gtccttcata ttatttctcc taatattagg
     6421 cgctgtgatc tatggatata ctcgaaacca tcaaccgtca gcaaaagaaa tctacaaaac
     6481 acgccttgtc gatcgaggag atgtcattca agtcatttct gcaaacggca cattaacacc
     6541 gcttgagctg gtcaatgtag gaacacaagt atccggcacg gttgcaaaaa tatatgtaga
     6601 ttttaatgat caggtcaaaa aaggacaaat attagctgaa ctggatcaag ctttacttcg
     6661 cgcgcaattg cttcaatcgg aagccaatta ccaaagtgca caagccacct tgaacatcac
     6721 tcaaaataag gcactgagag gtcgaactct gttacaaaag gggttcattt ctaaggaagc
     6781 tttagatgac cttgaacaac agcttgtact ggccaaagct caagttgcaa tcagcaaagc
     6841 aacggttgaa cgtgatcgag ccaatcttaa ctatagcgtg attcgctccc ctatttctgg
     6901 tgttgtcgtt gctcgagata tcaatgtagg ccaaaccgtt gctgcaaatt tccaaacacc
     6961 gatcttattt caaattgcaa aagatcttta ccaaatgcaa atcaatatca gtgtcgctga
     7021 agcggatatt ggtcaaattc aaacgacaca agaaatcaca ttcaatgtcg atgcgtttca
     7081 agaacgcgaa tttaccgcat ccgtcaaaca gatacgctta aaccccacta tccaagaaaa
     7141 tgtcgtaacg tataacgttg tcgcagcagt tgagaatcga gacggaattt tactccctgg
     7201 aatgactgct aatgttcgat ttgtcattaa tcacaaacga gatgtgctaa gaatctccaa
     7261 tgcagcgttg cgatataaac cgaatcgtga tgaagcacaa atcagtggtg ttaatcaggc
     7321 accgcaaggt acccagcttt atcgtttaaa aaatcaagtg cagcaaccag tccaagtcac
     7381 cacaggcgta acagatggtc acatgaccga gattgtttct ggtgatctga atgtgggtga
     7441 tgaaatcatt gtgagtgata acaaaagtag taaatcgaaa gaatcttctg ccagcaactt
     7501 tagattcaga atgttttaat gtctgtagtt aaccagcctg ctcccaaccc aatcttgatc
     7561 caaatcgatc gtttgtgtaa atcctatgaa ttgacggatc gttcgggaga gttgatcagc
     7621 caacctgtat tattcgatat cagcttaaca atcaatcaag gtgagtttat agccatcatg
     7681 gggcattctg gatctgggaa atcaacgctg atgaatatct taggttgcct tgatacgcca
     7741 accagtggca attattggct ttctggccgc aatgttgcca cgatgaatag taatgagtta
     7801 gcgcatgttc gtaatcaaaa tattgggttt gtgtttcaaa gctttaattt gttaaaacgc
     7861 atgacagcat tagagaatat cgctatcccc cttctttatg ctggcattgg acgcaaagaa
     7921 agtctaaaac gatcgcaagc aatgcttgat ttaataggat taggcaaata tagagatcat
     7981 cttccaaacc aactttctgg ggggcagcaa caacgcgttg caattagtcg tgcgctcatc
     8041 aaccaacctc agcttctatt agcggatgag cccacaggta atctcgatac tcaaactagt
     8101 cacgacatca tgactttatt tacacagctc aatcgcaatc aaggaattac tatcgtatta
     8161 attacccacg aagaagatat cgccgcgcac gcacaacgcc tcatccgatt aaaagatggt
     8221 cgtatcgtcc agaatgagat caaactgagt aactagcatg aatctactcg ttatcattgg
     8281 tgaagcgtta cgtgcattaa aactcaatcg attacgaacc gggttaacaa tgcttggcat
     8341 gattattggt gtcgctgcgg tcgtactgat gctatcgatt ggccaaggtg ctcaaattaa
     8401 tatcaataat acgattgctt ctatgggaag tcatttattg atcgttgttc caggcgctac
     8461 ttcttctggc ggtttacgat ttggtagtgg cagtgtgcgg acattgacta tcagcgatgc
     8521 acaagccgtt gctgaattac ccagcattga agcgacagcg cccgttatct caggaattgc
     8581 tcaactcagc tatagcgcta acaattggag cacctcaatc accggcgtaa cccccgatta
     8641 cttttctgtc agcaattggg aattagcaga tggttcaatc tttgccgacg cagatttacg
     8701 ttctgcgata cgcgttgcag tcctaggtag cgttaccgct gaaaatttat ttggtaatga
     8761 agatcccatt ggtaaaacct ttcgaatttc caataagccc tttctcgtgg taggaatact
     8821 agccgtaaaa gggcaaagct tgagtgggcg tgatcaagac gataatgtct ttgttccaat
     8881 cacaacggcg caacgccaga ttctgggcaa ccaattccca ggatcaatta atcatatgct
     8941 tgttcaagca aaatcatccg atacattaga cgaagcagaa gcagaaatta ctcagttatt
     9001 aaggcagcgg catcggatcg cagataaaat ggagaatgat tttacggtgc gtaacttaac
     9061 tgcattggca accgttgcaa gcactaccgc taaggtaatg gcctggatgc tcggttcaat
     9121 cgcctcgatc tcgctattag tcggcggaat cggtattatg aatatcatgc tcgtttctgt
     9181 tacggaacga acccgagaaa ttggtattcg tatggcaatt ggcgcaagct atcgaatcat
     9241 tctaatgcaa tttcttctag aagcgttggt tatctgtata ttaggcggac taataggagt
     9301 tgctttaggg attggtggcg cttggatggt gagccaatcc actgatatgc ccatcgtggt
     9361 cacgtttaat atgatcggat tagcatttat attcgtaaca gcaatcggta tattttttgg
     9421 attctaccca gcgagaaaag cagctgcttt aaagccagtc gatgcactcc gttacgagta
     9481 gagcgttaac acaccgttca agtgccatta ttcaatttta aatccactac caataaaaaa
     9541 accgccccca gtgatgaagg gcggtttcga aattgagtca attttcttat tttttatctt
     9601 cacccttatc aggcattgag tcacgttcca tcataatgct gggaggatat tgccctggtt
     9661 ttgggtcacc acacgcagtt aacataaacg ctgctagtac agctgcaaaa agtgcaaatc
     9721 tcattttagt ctcctattaa acaaatatga tgaattaact tattttattt aacttcttta
     9781 actgctctta gaccgttcat atctaacagc cacctcttta atagcagaat tacacaaggc
     9841 tgtccagaaa tttatgtatc aagaaaatga atgagcagtt attacctcaa cgatacaaga
     9901 gcttactcta taggtaacaa aactaactat ataataacca ctgtcaattt tttattaaca
     9961 tttaggctcg ttcaatttta ttttgtaatg aagaggttca atgacacatg attatcagtt
    10021 cagttttaat tgcttttcgt aatcttttta atttgaagat tcaatggata cttatttggc
    10081 cattaatcgt ctcatgcttg atttggatct tgctgagtca ctttctttgg gcccatgtat
    10141 cgggtggtat cgtagagttc atttcaacga ctcaaattaa agattggctt gaagaatcat
    10201 acttagtaaa aatcgcaaac agcctggaag gtcttgttaa tatccttctt ttcattgcgc
    10261 tcgttatcat cactacgcta atgataaccg ctttgtttgt tatgccttcc ctcatcaatt
    10321 ttgttgctaa acaatactat ccccatcttg aacggaagcg gggcggtagc gtcatgggta
    10381 atgtaaagca tgtactatca gcgctattgg tatttttatt gctttggagc attgcattgc
    10441 cgttgtggtt tttcggcata gggattgtga tttcatttat tgcggccgcg tatttcaatc
    10501 aacgattatt ctgctacgac gcactatccg aacacgctag tccagaggaa ttaacccagt
    10561 tgttggcttc taacaaaccc tctctctgga gtcttgggtt gctaacagga tttctgcagt
    10621 ttgtcccctt tcttaattta tttgcgccca catggactgc gctcgctttc gttcattacg
    10681 aacttggacg gcttgataaa ttacgggcta gtacaaagca agaaaacccg atagcaaact
    10741 tatcacttaa ccataagcaa agtgtggatg aggagtaaca accgattaaa ccggtttttt
    10801 tgcaagtttg cgctggagtg ttctgcgatg catattcaag atccgagccg ttaccgaaat
    10861 attgttatta ttctctgcaa gaatgcggtg aatatattcc cattctaagc gatccacgga
    10921 caatggatta ggactaatgg gaacatttgc ttcacccgat gttcgctcaa atgcagctaa
    10981 tatctcgttc acatcgacgg gtttggcaag gtaatgagtc gcgccgagtt taatggcttc
    11041 gatggcggtt gcgatactcg catagccggt cagcatgatg attcgggtcc ctgggtctaa
    11101 ttttctgatt ttttcgacga gcgtcaagcc agactcatct gcgagtttta aatcgataat
    11161 cgcgtattca ggcaccaacg actcaatttt ttccattgca gatgcaatgg tatgggcgca
    11221 aatcacttca aatccccgct tgaccattgc ttttgtaagc acttcgcaaa aaacactatc
    11281 atcatcgaca atgagcaatg aaggttgctc tatttctgtg ctattcagta cattcattga
    11341 cagttcattg cataagcaat tgcgttagga agattgaggt agtaggggga gtgtaagatg
    11401 aatacacgcc cccccttctg gatggttaaa taaatgtacg ctgcctccaa agcgctctat
    11461 attggcattg gcgagaaaca atccaatacc aaaaccttgg ccaggcgctt tactggtaaa
    11521 gaaggcttcc cctgcatgtt gcatcacttc gggtgttagg ccttcaccat catcatgaat
    11581 ttctaggttc agcgaattct gatcccaata actccgaatt ttgacttgat tcacagatgc
    11641 atcagccgca ttgttcaata aattaagaat agactggctg agtaactggg tattcatgat
    11701 atgcggtaca ggttgttgac catcacaact atactgaaac tttacagacg gacgaatgag
    11761 cgtccactta tccagcactt gcttcaagaa cacatcgaca gactgagtgc tcgcattctc
    11821 agttctggct tggccggcat ttgccaatag ctgagtcaat atatgcttgc aatgcgtaat
    11881 ttgatcgcgc aatgtataga tatttttttg atattcacca ttctcaggaa attcctgttg
    11941 gagctcacct gcaataacag ccatcgtcga caaaggtgtt cccagttcat gagccgcgcc
    12001 ggctgcaagt gtcccaagtg caataatttg ttcgttacac agcgcttgct ctcgcgcttc
    12061 agcaagctct ttatctcgtt gacgaatgga atggctcatt ctaacgataa accatgcaat
    12121 taagacggta ctcaatacaa aggtaaacca catgcctgaa acgtgcagat taaactccat
    12181 gagatgtgca ctatgatcgt cagagtgatc atggggtaac ggtacatatt caaacaataa
    12241 aatggtataa catgcgatgg aaattgcggc gattaaccag gtataacgcc agggtaagat
    12301 cgttgcagcg ataatcagtg gaagcaagta aaacgagatg aaaggattgg ttgatccccc
    12361 gctgaaatac aagagcgcag taagcgccaa tacatcgacc agaagctgta aaaaaaattc
    12421 tagattggta actggccaat tctgatgaag tcgataccaa gtcatcatat tgataatggc
    12481 tagcccgaca acgacagcaa tcaattcagc ccagggaata tcttgttcga taaaagaata
    12541 aaccaagaag aacaaagggc attgcaatat gattgcaata cttcttaaaa gaaatagccg
    12601 atgtaaattt ttgttgagtg gggaaagaag gagtcgattg aacatgtgac ttgcgcgcgc
    12661 attacttttc atacaattta attttaatgc tacattagcg taaattaacc aactaaacca
    12721 tgcgacaaat tgtcgcacat caagtatctt agccgctcaa attcccctcg atcccctggc
    12781 aggaacgtat ccaccgacaa ttgaatcgat ttactttcgc agcattaaca gcagtttttc
    12841 atggatgccg ccaaaaccgc cattactcat aattaaaata tgatcacctg catgcgccat
    12901 gagggcaacc gcattgatca attcttgcat ctggctgtat gtaactgctt tatgtttgat
    12961 tgattgtagc gctgacgtta catcccaatc gacttgatcg gtatagcaaa atatttgatc
    13021 tgcgatattt aagctttgag ccaaactatc tttccatacg cccattttca tggtgtttga
    13081 gcgaggttcc aaaacagcaa taattcgagc ctcaccaact ttagtgcgta acccctctaa
    13141 cgttgttcga attgcagtag gatgatgagc aaaatcgtcg tatacggtaa tattgttgac
    13201 gatacctttc acttccatac gacgcttgac atttttaaac tgcgataaag caagcaaacc
    13261 catttgagag ggaacgccag catggcgcgc tgccgcaatt gcagccaagg cattcattag
    13321 attatgttcc cccaataaat cccaatctaa tgtaccttga caggcctcgt tgaaataaac
    13381 ctcaattgca ccctgtgaag ataacttaac tgcccactct gtcggtactc caaaaaactc
    13441 gattggtgtc cagcagcctt gagcaataac ttgctgcaga ctactctcac gcccattcgc
    13501 aacgataagc ccgttgctag gaacggtccg gattaaatga tgaaactgtt tctgaatcga
    13561 tgctaaatca ggaaaaatat cagcatgatc atattcaagg ttatttaata ttaatgttct
    13621 gggatgataa tgaacaaatt ttgaacgctt atcaaagaaa gctgtgtcat attcatccgc
    13681 ttcaataaca aaaaaaggcg aagcttcccg gttcgttcca gaaacagatt ctccggttag
    13741 tcgtgctgag acaccaaaat tttctggtat ccctccaatc aaaaaacccg gatccaatcc
    13801 agcgaaagaa agtatccaag ctaacatcga gctcgttgtt gtttttccat gggtaccggc
    13861 tacagctagc acccatctct ttctaagaac atgctcagcg agccactgag ggcctgaact
    13921 ataaggtaaa ttctgattaa gaattgcttc catgagggga ttaccccgag tcacaacgtt
    13981 accaattaca tacaagtcag gattgagttt gagctgttcc gggctatacc cttcaattaa
    14041 ctctataccc agagaacgga gctgagtact catcggtggg tagacattaa gatcacagcc
    14101 ggtaacctta tgacccgatt cgcgcgccag cacagccaat ccacccataa atgttccaca
    14161 gataccaaga atatgaatat gcacgatggc ctctacccaa aaaatagtct atcgaattga
    14221 atacaaagtt gttcactcat catcttaaat gcgcttaaat tatactgacg attgatagtc
    14281 gttctgatag tagttcgaaa atgggttaac ccactatttc aataacacgg tatccagcct
    14341 gaataatgcg tcagatacaa gaaactacaa agtatagagg acgttttgca cccacaccaa
    14401 gtggtatgtt gcattttggc tcgctcgtta ctgcagtggg tagttatctt gacgcacgat
    14461 cacaccaagg tgaatggtta gttcgtatcg aggatgtcga tcaccctcgg gtgaaagcag
    14521 gtatatcgga aaaaatttta tccacattgg aacaattcgg tatggagtgg gatggtccag
    14581 tcatttacca aagtaaatgc atgaatcgtt accaagatgc gctccatttt ttggggtcat
    14641 taggattgac ttacccctgc agttgcacgc gtaaagaaat tgcagattcc gccttattgg
    14701 gattagaggg tatggtttat ccggggcatt gtcgtaatgg cctccctcca gaccataatc
    14761 aacgtcccca agcggtgaga ataaaaacga agcctgatag aattgaattt caagatcgtt
    14821 tgcaagggag cgtttaccaa tcaatcgcaa atgaaattgg cgattttgtt ttaagaagag
    14881 ccgatgggat ctatacctat caattggcag tcgtagctga tgatggttgg caaagtatca
    14941 ctcatatcgt gcgaggtgct gatttgttgg catcaacccc tcgccagatt tttctacagc
    15001 gtcttctagg atatcctacc ccctcctatc ttcatttacc tgtagtaaca actgctcaag
    15061 gtgagaaatt aagcaagcaa acacttgcac acccaattga tacggagaat ccactcttgc
    15121 aactcatggc agcgatcgat ttcttgggcc agcatcctcc atcagaatta attgaaggcg
    15181 atttgaattc gttctggcaa tgggctatca atcattggtc agtaacacgc atcgctcaag
    15241 tcacgtatgc tccctataac ccgtcactaa atatagtcaa tgaatagact tctcatcgcc
    15301 atttcgtgct acgcttgttc aattagctca ataaataacg attcaatcta ggaattcgag
    15361 gtgggtgtgt ggccgtttta ccgttttata tctcaaacaa aaaaaatcta aaaattgtca
    15421 tcgtaggcgg tggatatgct gggatagctg cactaacaac cattgctcgt tatcaaccca
    15481 gtgctgagat aacgctgatc gatccaaaaa tcgatcacat caaaattact catctccatg
    15541 aaacctttcg ctatcctttg actgattttt tgataccttt cactgatatc gaacaagaat
    15601 tcggctgtcg ccatctaccc ttagaattac ctttcagcga atcaacgcta ctacagtggg
    15661 aacgtgataa aactatttcg ataaataatg aatcaatccc atttgattat ctgctaatcg
    15721 cttcaggtgc gctgaataat aatgctgatc aagactcagg cgatgtaatg ggattgaacg
    15781 cttttatgaa gcatccggga tccgaaatac tgagtcaatt cttatcgaaa ccaggtactg
    15841 aagcacctat atccgttata gggggaggcg caaccggtat tcaattttta tttgagattg
    15901 agcatttctt acgctatcac aaatataaaa acaaattgcg cctgatcgat tctggagatc
    15961 aagtattaaa acagttcccc gatcggtttg cgcgctatgt tgaagccgcg atgaagaatc
    16021 taaatatcga atattaccca acgacttatt ttcgtgggca gcagcatagc caaataagat
    16081 tagaggataa aaaaacaagc caccaatttg aacttccttc aagtttaact ttcttatttt
    16141 cgggaaaaag ccaaggcagc gtattaaaag ccaatgcatt tggtcaggta atcgtagacc
    16201 aacaaatact gcaacatatt tttgtagcgg gtgactgtgc atactatcag tcgattggtt
    16261 ctaatgcttt aactgcccaa tcagcggttc gcaaaggaaa attagcagcc cgtaatcttt
    16321 taaggcattc aggcttgctt aaaatacttg agccttatct acaccgagat atcggctatg
    16381 tagttagtct tggacctgca gatgcgattg gatggttagc cttagaaaat aatgtggtta
    16441 caggcttagc agcttatact atcaaagaaa ttgtagaagc tcagtatgat ttattgctag
    16501 caggaatcga tacatatcta ctataaaaat atgatgcagg gagttattta atgttcaatt
    16561 tgtttgatga taagcctgtt gcaggaaagg ccagcacagt gattcagtcg ccagatgaca
    16621 agctatttaa ttttattggc cacgaactgc tgacgaatta ccctcgctgg tcccctgaag
    16681 taaaagaact tgagaaacta accgattctc cgttgggatt aggaacactg tgccgccagg
    16741 ttcgagttga tcagggaaat cgatcggagt cgacattcaa aatcgcggta ttcgaaccca
    16801 acaaacaaat ttgctttaat ggcgtgtcaa acccgtttcg ctgtgattat tccctcgagt
    16861 caaaaaatca agaatcaact caaattacct ttacttttga attactaagc ttagaactgc
    16921 atatgagacc ctttgaaaaa ctcattcgaa ttgccgtgca agatggtgca gaaaggacag
    16981 ttagaaacat taagcgatta attgaattca acacgattta attcagcttg aaaagaataa
    17041 cccgaagaag cttatacaac cctttatatt gaattggaga aaaagtatgg attttatcgt
    17101 tgaaaaagat cctggattaa ttccacaagt attatctaac atccttgatt cctgcgtaaa
    17161 tggcattact ttggctgatc ctgatcagga ggacttaccc cttgtttatg caaataaagc
    17221 gttcgaaaca ataaccggtt atacacaagc tgaaaccatt gggaaaaatt gtcgattctt
    17281 gcaaggcaat gatcgagatc agcaagagcg tcatcagtta cgagaggctg ttttaaataa
    17341 gcaatccatt gagataacgc tgagaaatta tcgaaaaaac ggcgaaatgt tttataacca
    17401 tctcgttata acgcctttgt ttgattcgaa aggaaatgtt ttatactacc tcggtgtaca
    17461 attcgatgta acacagcaga aaaaagctga ggaagaaatt aaacgcctta cagagcagct
    17521 agcgaaatta tcaaagtaaa ttaatttttt tgattactac tagcaaaaca ggtcttaaat
    17581 gagtatcatc agcgcaaatc aaatgtgact taccatgaca agttacagtt tttattgatt
    17641 gacttcatca aataaccgga agcgtggccg agcggtttaa ggcactggtc ttgaaaacca
    17701 gcgatgggca actatccgtg agttcgaatc tcaccgcttc cgccaatcat tcactatcca
    17761 tcccgtttta accccaatcc gctcccatca aaacatttct ggtttaaagc ctagtttttt
    17821 gggatcgctg cacaga
//
LOCUS       DAOQKQ010000032        17537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_1458_length_17537_cov_5.149010, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000032 DAOQKQ010000000
VERSION     DAOQKQ010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 17537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17537
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1458_length_17537_cov_5.149010"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..2334
                     /locus_tag="PKZ49_03445"
     CDS             100..2334
                     /locus_tag="PKZ49_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HND35646.1"
                     /translation="MEKEIKKQCDRCFRFDNSKIIWLGIVIVVGILSACSRPIWNSPY
                     PSEDASKNILYNAFAERPKHLDPVQSYSSNEILLTAQIYQPPLQYHYLKRPFTLIPQT
                     THGMPLVKYIDEIGNESHAEKDITNIAYSIYEISIKPDIRYQPHPAFARNDQGNLRYH
                     ALTQKETESIFKLSDFTALGSRELLAEDYAYQIKRLAHPKLHSPIFGLMSEYIVGLKE
                     YAETLRKIADTLPKGTHLDLRDFPLEGVEIVDASTYRIKIKGKYPQFIYWLAMPFFAP
                     IPWEAERFYSQPGLAEKNITLDWYPVGTGAYMLVENNPNRIMVLERNPYFQGEYYPAD
                     GMPEDEESGLLADAGKPLPFIDRVIFSREKENIPRWNKFLQGYYDASGIGSDSFDQAV
                     QLTSHGEATVTEEMQQQGIRLETAVAASTYYMGFNMLDPVVGGRTDQEKAAARKLRQA
                     ISIAVDYEEYVSIFANGRGIPAQGLIPPGIMGYREGKEGMNDIVHQWIDGRVKRRSIE
                     EAKKLLAEAGYPDGIDIKTGAPLVLYFDATSRSADDKSILDWMRKQFKKINIQLVVRS
                     TDYNRFQDKMRKGNAQIFEWGWNADYPDPENFFFLLYGPQSKAGNHSGNNAANYDNPE
                     YNQLFERMEKMDNGPERQAIIDQMVEILRYDAPWLWGYHPKDYALYHAWFQNVKPNKM
                     SYNNMKYLRINAELRERKQSEWNQPVLWPIGLAVIILMLSLLPGFIAYRQKERKTEAK
                     QLVG"
     gene            2346..3323
                     /locus_tag="PKZ49_03450"
     CDS             2346..3323
                     /locus_tag="PKZ49_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HND35647.1"
                     /translation="MLNYIIRRICYAIPILIGVNLITFVLFFMVNTPDDMARMHLGVK
                     YVTDDAIEKWKQDRGYDKPLIYNQTAEGFSKLTQTIFFEKSVAMFVFDFGQADDGRDI
                     SHEIKSRMVPSLAIALPVFLLGIWVYISLALMMVFFRMTYIDFWGVVLCVALMSISSL
                     FYIIGGQFLISKIWHLVPISGYGTGLDASKFLLLPVVIGVISSIGANTRWYRTIFLEE
                     MNKEYVRTARAKGLPESTVLFKHVLKNALIPILTGAVVTIPLLFLGSLIAESFFGIPG
                     LGSYTIEAINSQDFAIVRAMVFIGSFLYIIGLILTDISYTLVDPRIRLM"
     gene            complement(3400..3645)
                     /locus_tag="PKZ49_03455"
     CDS             complement(3400..3645)
                     /locus_tag="PKZ49_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35648.1"
                     /translation="MDESTDVLLISILAIPFVIAIQAWVKKRKDSRRNEEGEEEFKTT
                     REALFAFLLEGLAIVGGLAILIAATSGILRYVINTYS"
     gene            3837..5150
                     /gene="aroA"
                     /locus_tag="PKZ49_03460"
     CDS             3837..5150
                     /gene="aroA"
                     /locus_tag="PKZ49_03460"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HND35649.1"
                     /translation="MGWLDLTISQCALGTVKLPGSKSISNRVLLLAALAQGDTLVQNL
                     LVSDDTRHMLDALKMLGISITQIDTVDYQIQGSGGKFPNQEAKLFLGNAGTAFRPLLA
                     VLAIMGGHYYLSGVSRMHERPIGDLVDALKQLHAEIQYLGNPGYPPLEIKLAEIHNTP
                     VTIKGNVSSQFLSGLLMALPLIKEATSIIVDGELISKSYVSLTLAQMEKFGVHVINEN
                     WQRFILPGLQTYQSPGKIYVEGDASSASYFLAAGAIGGGPVRVVGVDKDSAQGEIKFA
                     EALERMGAKITYGENWVESAAISCNPTTRYLSAIDLDCNHIPDTAMTLAVLALFAEGT
                     TKLRNIASWRVKETDRIAAMATELRKLGASVEDGQDYLVITPPKSITPNVLIDTYDDH
                     RIAMCFSLVSFAVPVRINNPECVAKTFPDYFQRFTAIAHPPIGSN"
     gene            5180..5845
                     /gene="cmk"
                     /locus_tag="PKZ49_03465"
     CDS             5180..5845
                     /gene="cmk"
                     /locus_tag="PKZ49_03465"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HND35650.1"
                     /translation="MDKIPVITIDGPSASGKGTVAQRVAKQLTFHYLDSGALYRLVAL
                     QAMRSKTDITDEHALAHIAENLVVCFNDSTIILNQEDVTDLIRSEECGKYASRIAAFP
                     LVRTMLLNKQRAFRQQPGLVTDGRDMGSVVFPNASLKIFLTASAEERAQRRYKQLIEK
                     GINANINILQQDIYERDARDSKREIAPLQQFADTKLLDTTFLSIDQAVNEVVKWYQAI
                     RNT"
     gene            5953..7668
                     /gene="rpsA"
                     /locus_tag="PKZ49_03470"
     CDS             5953..7668
                     /gene="rpsA"
                     /locus_tag="PKZ49_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HND35651.1"
                     /translation="MTTAANLKDSSENFAQLFEESLARKEMRVGEVITAEIVRVDYNF
                     VVVNAGLKSESYIPIDEFKNDRGEIEVKAGDFVSVAIDALEDGYGETRLSRDKARKLN
                     AWHDLEEAMEKGTIVSGMVNGKVKGGLTAMVNGIRAFLPGSLVDIRPVKDTTPYENKE
                     MEFKVIKLDKKRNNVVISRRAVLEETQGADRESLLANLQEGSTVHGIIKNITDYGAFV
                     DLGGIDGLLHITDLAWRRVKHPSEVINVGDEVTAKVLKFDQEKNRVSLGLKQLTEDPW
                     VGLSKRYPQGTRLFGKVTNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVYPSKIVQLG
                     DEVEVMIIEIDEERRRISLGMKQCKPNPWEEFASGHEKGEKVSGQIKSITDFGIFIGL
                     PGNIDGLVHLSDLSWSQSGEEAIANYKKGDEVEAVVLSIDVEKERISLGIKQLEGDPF
                     TIYVAEHDKNSIVKGKVKAIDPKGAVIGLSEEVDGYLRASEITRDKVEDIRTLLKEGD
                     EVESMIINVDRKNRNINLSIKAKEQQEESSALQMVSSKTPASTGTTNLGALLKAKMDN
                     KNTEE"
     gene            7685..7978
                     /locus_tag="PKZ49_03475"
     CDS             7685..7978
                     /locus_tag="PKZ49_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HND35652.1"
                     /translation="MTKSELISRLSARYPQLLTKDTELAVKMILDAMANSLSKGERIE
                     IRGFGSFDLNFRPARVGRNPKSGEKVLVPEKYVPHFKAGKKMREHVDFDKEKS"
     gene            8035..8244
                     /locus_tag="PKZ49_03480"
     CDS             8035..8244
                     /locus_tag="PKZ49_03480"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HND35653.1"
                     /translation="MYIFIWLAGFVFFLLLLTFSAKNTDLVTVNYYFDFHWQVPLVVL
                     FLIFFALGSCFGYLSCFVKHLRKKT"
     gene            8263..8973
                     /gene="pyrF"
                     /locus_tag="PKZ49_03485"
     CDS             8263..8973
                     /gene="pyrF"
                     /locus_tag="PKZ49_03485"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HND35654.1"
                     /translation="MSEPRIIVALDYSDKKQALDLVNQLDKTCCRVKVGKELFTAAGP
                     QLVEQLMMLGFEVFLDLKFHDIPNTVAQACKVAANLGVWMVNVHASGGKKMLLAARDA
                     LPPGKTKLIAVTVLTSLDQFALNEIGWYRAPMDIATQLASLTRDCGLDGIVCSAAEAA
                     IIRKNIPQKNFWLVTPGIRPADSAQHDQARVTTAKVAILSGADYLVIGRPITQSANPM
                     ATLQQLNHEILSAYSAIN"
     gene            complement(9009..9995)
                     /locus_tag="PKZ49_03490"
     CDS             complement(9009..9995)
                     /locus_tag="PKZ49_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HND35655.1"
                     /translation="MRFHLIIAYILLNFSTLVIAQSPELGNSSFDKFEFALIGDVPYS
                     RDDFDKLDRLIHEINTDPRLTWVLHAGDIKNGISVCSDRMFIDRLQRFQQFKIPFILT
                     PGDNEWTDCHRFFTGSYNPLERLARLRGLFYANPGNSLGQKTVTLRTQASDPHFSEYV
                     ENLRWRKNHVVFATVHVVGSNNGWAQFSNRTEADDMEAKRRTEAAIVWIKQTFEEARN
                     TDARGVFVLFHADLNFEYAKGSHARLGFDGIIEVFEQESAQYLRPILLAHGDSHRFRV
                     DKPLQNNKKQTINHVTRVETFGASNVHWVRVAVDPASAEVFTIQKQIIKKNR"
     gene            complement(10046..10888)
                     /locus_tag="PKZ49_03495"
     CDS             complement(10046..10888)
                     /locus_tag="PKZ49_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HND35656.1"
                     /translation="MLWKGRRQSSNIEDRRGQYARRGGAIGGGSIIIALIAVFIFGQD
                     PLVVLQELQQQAGMVETSNSNYEPNPEEQQVFELISVVLADTEDTWNQIFQKAGYDYQ
                     APKLVLYSGMTPTACGTGQAASGPFYCPGDQKIYLDLSFLSELQRMGASGDLAVAYVL
                     AHEVGHHIQTLTGVSTKVRNLQNQTNQVGANAIQVKMELQADCLAGIWVNHTQKRTQF
                     LEQGDIEEALQAASAVGDDQLMRKAGLPPRREAFTHGSSKERMSWFKQGLKTGDLAAC
                     ETGI"
     gene            complement(10982..11689)
                     /locus_tag="PKZ49_03500"
     CDS             complement(10982..11689)
                     /locus_tag="PKZ49_03500"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HND35657.1"
                     /translation="MKPVVIIRFFPIEGPGYFATFLDNNHIPWKVVKVDQAETIPSTL
                     QAFSGLVLMGGPMSVNDDLPWIPQVLGLIQQAIELNIPVLGHCLGGQLMTKAMGGVVT
                     QGPIKELGWGKVDVMDHPVAKAWLGDITEFEAFHWHGETFSIPEKATLLFSSPYCAHQ
                     AFATDIHLAMQCHVEVTEEMVPTWSTFNTAEIEQNLSSPAVQTAPAMQERLPQRIAAL
                     NGVADQLYRKWIQALKA"
     gene            11843..13228
                     /locus_tag="PKZ49_03505"
     CDS             11843..13228
                     /locus_tag="PKZ49_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HND35658.1"
                     /translation="MNVNQKNFSDLVHLEQLRHIETNKGSELSYASVADMRLHSAREF
                     SYRYLLNHLTTRAKRLIHDNALTEAVEQPHKLFVRATRICVIIAAILGALAVGHAVGE
                     SATLNIYWLITVLLGFNFLSILLWILGISFNLQSLRNGVVAQLACWLPYRNKDSDSIR
                     ALATRAWWETCLTGSIGKWRISTLTHQFWLAYLGAGLTFLILLMMAKQYDFIWGTTLL
                     PDNSLPKLTQFLGTPVEMIGFRSPDTQQIASSRVGLAIHDAETRSAWASFLLGMLLVY
                     GLFPRIILLSISLFMLRLAESRYKLDLYLPYYITLRQQLMSHEFEAQVIDADPQIAQE
                     RPVQMIKPAASIAPANALVVGIELEESVQWPQTMKCNLNVVDQASFDAAAATIKQSNQ
                     PLIIGASLQRLPDRGVQRMIHELVTSTSQPVWLYLLRKDTTASVNDARKLAWFRLAEA
                     CTIPAEHVIYQ"
     gene            13240..14643
                     /locus_tag="PKZ49_03510"
     CDS             13240..14643
                     /locus_tag="PKZ49_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3482 domain-containing protein"
                     /protein_id="HND35659.1"
                     /translation="MSKPSDQALDSLLNIAVVGHTNTGKTSLIRTMLRSTDFGKIEDA
                     AGTTRHVERATIYAGSEAILNLHDTPGIEDAHGLLKRLKSTDSRNKSPASPAEILESF
                     VNSVSVSDAFEQEAKVVRQVLRSDVLLYIIDVREPILEKYFIELEILSKAMKPIIPVF
                     NFIAGHKDELAAWRKKLATINIHASLEFDTVAFSFEAEKRLYQKIQTLVEAHYDRLQK
                     LIDYRSKVWDQLCFSAAKRIVELIVSVSCYRLSKIESPEDSHQSPAATQLQDFVRKAE
                     QRCLEDLLAIFNFSEKDIALQKIPVSNGHWQLDIFAPGVLKAYGLDAGSAALKGAAAG
                     AGIDLMVGGLSLGAASALGALAGTGWSTFKRYGKEIKAKIQGTEWLCADENTLKILYL
                     RQQQLLSKLMQRGHAAYDPDHIEETVTGELPAKWSEIVSILQQNPLWQKPSPQRDQNQ
                     QFQTLQSQLINALLDNK"
     gene            14735..15514
                     /locus_tag="PKZ49_03515"
     CDS             14735..15514
                     /locus_tag="PKZ49_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35660.1"
                     /translation="MRHIGCLIVVRSHISTTPTYRFRVHRAATDCSQDWRVWIEGSEV
                     YLAPRSVGHQYKASFHSSGQCQVGLSSSLRESLIGDPLWEGKSRLFSVWNVPTTFSRN
                     ERVILVELFFPGSYLDNVTSKPGKSVELLECPDNHVVSICLLKSSLQSGVSFKSSNSS
                     FRELSRLPCTDGCSLSVLYRVLPENDEYRNYLRYRYWSHYLAEPSSDGLTFGTWGDIP
                     TSADVRALIWDGRTEPKQWHEVSVRKLHALGPPQTDTHGVR"
     gene            complement(15704..16786)
                     /locus_tag="PKZ49_03520"
     CDS             complement(15704..16786)
                     /locus_tag="PKZ49_03520"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HND35661.1"
                     /translation="MVVNKTKDRAIGAIMGAFIGDALGLGPHWYYDLNELRRDYGDWI
                     DNYTDPRPNRYHAGCKAGQLSQVGFILVLTLRSLVECGEYNKDDFCRRMDEELFPLLD
                     GTPMSGPGSYTSQSIREAWRCRVQHKLPWGQTGSHADTTEAIERTLAIAVRYAFQPNQ
                     LAAAITDNTMLTQIDHTVVSMTVAYGAVLGLLIQGHLLNTDLSGKLMELVKDGKLPFH
                     AVTRDALQPPRPGDPDPPRAGRFASPDALLTPSYMAAAYENPEIRIEPAWKVSIVYGM
                     PCAIYHQLPAAYYLAARFKDDFESAVLHAINGGGQNQARAILTGALTGAQTGLTGIPQ
                     RFLDGLDGCADLLRLAEHLAAKVGNF"
     gene            complement(16954..>17537)
                     /locus_tag="PKZ49_03525"
     CDS             complement(16954..>17537)
                     /locus_tag="PKZ49_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35662.1"
                     /translation="IYNLLFCDNLAAFKPKEGERAATWQIVLFAEPADRSALLALAND
                     VTQESRIRYLAYTRLRLLGENVPTKVLLGVIVEVSLADGLDALAAYSDGGVRYINHSG
                     ELVFFEGSTELEPHVKKLLAASEPVVARIGPWKKPRRSPPESDNVRLTFLVSDGLYFG
                     EGPMSIMQHEEMAGPVIQSATTLLQTIVSRASE"
BASE COUNT         4976 a         4042 c         3719 g         4800 t
ORIGIN      
        1 gcatcaagat caaaatttgg taacggcacc cggatggcct gcccatccag attggctcgc
       61 aaagtttcta gatctactgg gaacgagaat caaaccctaa tggaaaaaga aatcaagaag
      121 cagtgtgatc gttgttttag gtttgataac agcaaaatta tttggcttgg gatcgtaatc
      181 gttgttggga tactatctgc ctgtagtcga ccaatttgga atagccccta ccccagtgaa
      241 gacgcaagta agaatattct ctacaacgcg tttgctgaac gacctaagca cctggatccc
      301 gttcaatctt acagttccaa tgaaatttta ctcaccgcac agatttatca acccccacta
      361 caatatcact acctaaaacg cccttttacg ctgattccac aaacaaccca tggaatgcct
      421 ctcgtcaaat acattgatga aataggcaat gaatcacacg cggaaaaaga tattacaaat
      481 atcgcctatt cgatttacga aatttcgata aaacctgata ttcgctatca accccatcca
      541 gcatttgcta gaaacgatca gggaaacctt cgctatcatg cattaacgca aaaagaaact
      601 gagtcaatct ttaaactgag cgactttacc gcattgggct cccgcgaatt gctagcagaa
      661 gactatgctt accaaatcaa acgattggcg catcctaaat tacattcccc catttttggc
      721 ttaatgtcgg aatatattgt aggactcaag gagtatgctg aaacactgag aaaaatagca
      781 gatacattac ctaaaggcac acacttggat ctacgcgatt ttccattaga aggagtggaa
      841 atcgttgatg cttctaccta ccgaatcaaa atcaaaggaa aataccctca attcatctat
      901 tggctagcta tgcctttttt tgcgcccatt ccttgggaag cagaacgatt ttattctcag
      961 ccagggttag ctgaaaaaaa tatcacgctc gattggtatc cagttggaac cggtgcttat
     1021 atgcttgtag agaacaaccc taatcgcatc atggtactcg aacgtaatcc ctactttcag
     1081 ggtgaatatt atccagcaga cggtatgcct gaagatgagg aaagtggttt actcgctgat
     1141 gcaggaaaac ctttaccctt catcgacaga gtcattttta gtcgtgaaaa agaaaatata
     1201 ccgcgctgga ataaattttt acaaggttat tacgatgcat ccgggattgg ttcggatagt
     1261 tttgatcagg cagttcagct aacaagtcat ggcgaagcaa cggtaacaga ggaaatgcaa
     1321 caacaaggta ttcggctgga aaccgctgtt gctgcatcga cctactatat gggttttaat
     1381 atgcttgatc ctgttgtagg aggccgaaca gatcaagaaa aggctgctgc tagaaaatta
     1441 cggcaagcca tttcaattgc ggttgactat gaggagtatg tctccatttt tgccaatggg
     1501 agaggaatac ccgcccaggg gcttatccct cccggcatca tgggctatcg agaaggtaaa
     1561 gaaggtatga acgatattgt tcaccagtgg attgatggtc gtgttaagcg aagatccatt
     1621 gaagaagcca aaaaactact tgctgaggct ggataccctg atggaattga cattaaaacc
     1681 ggcgccccac ttgttctcta ttttgatgct acttcacgga gcgccgatga taaatccata
     1741 cttgattgga tgcgcaagca attcaaaaaa atcaatatcc aattggttgt aagaagcaca
     1801 gattacaatc gattccaaga caaaatgcgc aaaggaaacg cacaaatctt cgaatggggt
     1861 tggaatgctg attatcctga tcccgaaaat tttttctttc tgctgtatgg acctcaaagt
     1921 aaggccggca accatagcgg taataatgct gccaattacg ataatccaga atataaccag
     1981 ttatttgaac gcatggagaa aatggataat ggccccgaac ggcaagcaat tatcgatcag
     2041 atggtggaaa ttctacgcta cgatgcccct tggttatggg ggtatcaccc caaagactat
     2101 gcgctttatc acgcatggtt tcaaaatgta aaacccaata agatgtctta taacaatatg
     2161 aaatacttgc gcattaatgc tgaattacga gagcgcaagc aaagcgagtg gaatcagccg
     2221 gtgctttggc ccattggcct cgctgtaatt atactcatgc tgagcttact accagggttc
     2281 attgcatacc gccaaaaaga acgtaaaact gaggccaagc aacttgtagg ttaaaaaagt
     2341 aaacaatgct taattatatt attcgccgta tctgttacgc gattccaatt ttgattggcg
     2401 tgaatctcat tacattcgtt ttgtttttta tggtcaatac acccgacgac atggcaagaa
     2461 tgcaccttgg ggtcaagtat gtcaccgatg acgcgatcga aaagtggaag caggatcgag
     2521 gttatgataa gccactgatt tataaccaaa cggctgaagg cttttcaaag ctaacacaga
     2581 ccattttctt cgaaaaatca gttgccatgt ttgtgtttga ttttggtcaa gccgatgatg
     2641 gccgcgacat cagccatgaa attaaatctc gcatggttcc tagcttagcc attgcattac
     2701 cggtattttt gttgggaata tgggtctaca tctcacttgc cttgatgatg gttttctttc
     2761 gcatgactta tatcgacttc tggggtgtcg tactatgcgt cgcactgatg tcgatttcaa
     2821 gcctttttta tatcattggc ggccaattct taatcagcaa aatctggcat ctagtcccta
     2881 tttcaggcta tggtactgga ctcgacgcga gtaaattttt gttattacct gtagtaattg
     2941 gggtaatcag tagtataggc gctaatactc gctggtatcg aacaatcttt ctggaggaaa
     3001 tgaacaaaga atatgttcgt accgcacgtg ccaaaggctt acctgaaagt actgtcctat
     3061 ttaaacacgt gcttaaaaat gccctcattc caattctaac gggcgcagtc gtcacgattc
     3121 cgctcttatt tctaggcagc cttatcgctg agtcattttt tggtattccc ggcctcggca
     3181 gctataccat agaggctatc aattctcaag attttgccat cgttcgtgcg atggtattca
     3241 ttggttcgtt tctttatatc atcggcctta ttctgacaga tatttcctac acgctagtcg
     3301 atcccagaat aaggctaatg tagtgatcag cctttcaaga agtcaaatta atagcctact
     3361 accaactatc aactcagcta atcaacaagt atctcaagtc taactataag tgttaatcac
     3421 atacctaaga ataccgctag tagctgcaat caaaatggct agaccgccta caattgctag
     3481 cccttcaagc aggaaagcaa ataaagcttc gcgcgttgtt ttaaattctt cttcgccctc
     3541 ttcgtttcgt cgactatctt tccgtttttt cacccacgct tgaatcgcta tgacaaacgg
     3601 gattgccaag atagaaatca gtaacacatc tgtagattca tccattcgat cgttccttta
     3661 agctataatt attattggtt gaaaacatta actttttcta gattattttt cattatagca
     3721 gacatcgtaa tacctgctct ctatctctat cgttccccct tattgaatgc tttgttcttc
     3781 ctttaaaaaa gtagcgttaa tgactagaaa aagaattaaa aaaatttatt taaactatgg
     3841 gatggcttga tctaactatt tctcagtgtg cgcttggaac cgttaaactg ccgggctcta
     3901 aaagtatctc caatcgggtc ttattgctcg cagcacttgc tcaaggggat acactcgtgc
     3961 aaaacctcct ggtatctgat gatacgagac acatgctcga tgcgcttaaa atgctgggca
     4021 ttagcatcac gcaaatcgat actgtggatt atcagattca aggaagcggt ggaaagtttc
     4081 ctaatcagga agctaaatta tttctaggaa atgcgggaac cgcttttcgt ccccttttag
     4141 cggtactcgc aatcatgggt ggtcactatt atctctcagg tgtctcacgc atgcatgagc
     4201 gtccaatcgg ggatctcgtc gatgcgttaa aacaactcca cgctgaaatt caataccttg
     4261 gcaatccagg ctacccccct ctggaaatta agttagcaga aatacacaat acaccagtta
     4321 cgataaaagg gaacgtatcc agtcaatttc tgagtggatt attaatggcg ctcccactta
     4381 tcaaagaagc gaccagtatt atcgtggacg gcgagctcat ttcaaaatcc tatgtgagcc
     4441 tcaccttagc acagatggaa aaatttggag tccatgtcat taatgagaac tggcagcgct
     4501 ttattttacc tggcctacaa acctaccaaa gcccagggaa aatttatgtc gagggcgatg
     4561 cgtcttctgc ttcctacttc ctcgctgcag gtgctattgg aggaggccca gtacgagttg
     4621 tcggtgtcga caaagatagc gcgcaaggcg aaataaaatt tgccgaagca ctcgaaagaa
     4681 tgggtgcaaa aattacctat ggtgaaaact gggtggaatc tgcggccatt tcatgcaacc
     4741 ccacaacacg atacttaagt gccatcgatc tggattgcaa ccatattcct gataccgcaa
     4801 tgactttagc agtgcttgct ctctttgcag agggaacgac aaaattacgt aatatcgcaa
     4861 gctggcgagt caaagaaaca gaccgcattg ctgcgatggc cactgaattg cgtaaactcg
     4921 gtgcatctgt tgaagatggg caagattact tagtgattac cccccccaaa tccattacgc
     4981 ccaatgtgtt aatcgatacc tatgatgatc accgcattgc tatgtgcttt tcgctcgttt
     5041 cattcgccgt gccggttcga attaataacc ctgaatgtgt cgctaaaact tttcctgatt
     5101 attttcaacg attcaccgct attgctcacc cgccgatcgg gtcaaattaa cttatttttt
     5161 tcaagagatc tcttttaaaa tggataaaat cccagtcatc accatcgatg gcccttccgc
     5221 atcggggaaa ggaaccgtag cacaacgtgt agcaaagcaa ctcacattcc actatttgga
     5281 tagtggagcg ctctaccgcc ttgttgcatt acaagccatg cgttcaaaga ccgatatcac
     5341 cgacgaacac gcattggctc acatcgcaga aaatctagta gtttgtttca atgattccac
     5401 tatcatactt aatcaagaag atgtcaccga tttgatacgc agtgaagaat gcggcaaata
     5461 cgcgtctcgt attgcggcct ttccactcgt cagaacgatg ttattaaata agcaacgtgc
     5521 attccggcag caaccaggat tggtcaccga cggacgggac atgggatccg ttgttttccc
     5581 caatgcatcc cttaagattt ttcttactgc aagcgccgaa gaacgtgccc aaaggcgcta
     5641 taagcagttg atcgagaaag gaataaatgc taacatcaat atcttacagc aagatattta
     5701 cgaacgagat gcgcgtgata gcaaacgaga aattgctccc ttgcaacaat ttgcagatac
     5761 gaagttactc gatacaactt ttctgagtat cgatcaagcc gtgaacgaag ttgttaagtg
     5821 gtatcaagca atacgcaata cataaatctt cgttctttct ttaagtaaat aatctttttt
     5881 tcaacccaat aaccaacaat cccctgctta taaggggatt ggattgatta acaaattagg
     5941 taatttttta taatgactac tgccgctaac ctgaaagact cctcagaaaa ttttgctcaa
     6001 ctctttgaag aaagtctcgc acgtaaagaa atgcgtgtgg gagaagtaat tacagcagaa
     6061 atcgttcgtg ttgattacaa cttcgtcgtt gttaacgcgg gcttaaagtc tgaaagttat
     6121 atccctatcg atgaatttaa gaatgaccgg ggagaaatcg aggttaaagc cggtgacttt
     6181 gttagcgtgg ctattgatgc acttgaagat ggttatggag aaacaagact atcgcgggat
     6241 aaagcaagaa agttaaatgc gtggcatgat cttgaagaag ccatggagaa aggaacgatt
     6301 gtcagtggga tggtaaacgg aaaagttaaa ggcggtctca ctgctatggt taatgggatt
     6361 cgcgctttcc ttccgggttc attggttgat attcgcccag ttaaagatac gactccgtat
     6421 gaaaacaaag aaatggagtt caaggttatt aaactggaca aaaaacgcaa taatgttgtt
     6481 atttctcggc gtgcagtgct tgaggaaaca caaggtgcag atcgcgaatc attgcttgca
     6541 aacctccaag aaggttccac tgttcatggc attatcaaaa atattaccga ttatggcgcc
     6601 tttgtcgatt taggtggaat tgatggtcta ttgcatatca ctgacttagc atggcgtcgc
     6661 gttaaacatc cttcagaagt tatcaatgtc ggggatgaag tgactgcgaa agtcttaaaa
     6721 tttgatcaag aaaaaaatcg tgtgtcgctt ggattgaaac aattaacaga agacccatgg
     6781 gttggactct ccaaacgcta cccacaaggc actcgcttat ttggcaaagt aactaatcta
     6841 accgattatg gtgcttttgt tgagatagaa caaggaatcg aagggttagt gcatgtttcc
     6901 gaaatggatt ggacgaataa gaatgtctat ccttcaaaaa ttgttcaact gggcgatgaa
     6961 gttgaagtga tgattatcga gatcgatgaa gaacgccgca gaatttcgct aggcatgaaa
     7021 caatgcaaac caaatccttg ggaagaattt gcttctggcc atgaaaaggg tgaaaaagtt
     7081 tcaggtcaaa tcaaatcgat taccgatttc ggtattttta ttggcttacc tggcaatatc
     7141 gatggcttgg tccatttgtc tgatctttct tggagccaat ccggtgaaga agcaattgct
     7201 aattataaaa agggagacga agtagaagcc gtcgtcctat cgatcgatgt ggaaaaagaa
     7261 cgaatttctc ttggcatcaa gcaacttgaa ggagatcctt ttactattta tgtggcagag
     7321 catgataaaa atagtattgt gaagggtaaa gttaaagcaa ttgatccgaa aggagccgtt
     7381 atcggcttat ctgaagaagt tgacggctat ttacgcgcat ctgaaatcac gcgtgataaa
     7441 gttgaggata ttcgtaccct acttaaagag ggtgatgaag tcgaatcaat gattattaat
     7501 gtcgatcgca aaaatcgtaa tatcaatctc tcaatcaaag ccaaagagca gcaagaagaa
     7561 agtagtgccc tccaaatggt tagttcaaaa acacctgcaa gtacaggtac aaccaatctc
     7621 ggtgccttgc taaaggcaaa aatggataat aaaaacacag aagaataata ggtaagaggc
     7681 atcaatgaca aaatctgagt tgatttcgag gctctcagcc cggtatcctc aattattgac
     7741 caaggatacc gagttagcag taaaaatgat actggacgcc atggccaata gcttatcgaa
     7801 aggcgaacgt attgaaattc gtggatttgg cagctttgat ctaaatttcc ggcctgcgcg
     7861 tgtaggccgg aatcccaagt ctggtgaaaa agttctagtt cctgagaaat atgttccaca
     7921 cttcaaagcg ggtaaaaaga tgcgagaaca tgttgatttc gacaaagaaa agtcttaatt
     7981 tgaaacctac atagagcgcg ttcctatcta ctcaatctta aaggaaagac gattatgtat
     8041 atcttcattt ggttagcagg ttttgtgttt ttcttattgt tattaacatt ctctgctaag
     8101 aatactgatc tcgtaacagt taactattat ttcgattttc attggcaagt tccactcgtc
     8161 gtgttatttc tgatcttttt tgcactaggc tcatgttttg ggtatttatc ttgcttcgtt
     8221 aagcatttgc gtaaaaaaac ttagcgatct actcgccctt atatgagtga acctcgtatt
     8281 atcgttgcac ttgattattc ggataagaaa caagctttag atttagtcaa tcaattagat
     8341 aaaacctgct gccgcgtcaa agtaggaaaa gaattattta ctgcagcagg acctcaatta
     8401 gtcgagcagc tgatgatgct cggctttgag gtgttcttag atttgaaatt ccacgatatt
     8461 cccaataccg ttgcacaggc atgcaaagtt gcagcaaacc taggcgtttg gatggtcaac
     8521 gttcacgcat cgggagggaa aaaaatgttg ctcgcagcac gcgatgcgct accacccggc
     8581 aaaaccaaat taattgcagt aaccgtgctc accagcttag accaattcgc cttgaatgaa
     8641 atcggttggt atagagcacc aatggatatt gccactcagc tcgcttcatt aacccgtgat
     8701 tgtggcttgg atggtatcgt ttgttcggct gctgaagctg ctatcattcg gaaaaatatc
     8761 cctcaaaaga acttttggct cgtgacccca ggaattcgtc cagcggatag cgctcagcac
     8821 gatcaagctc gcgttacaac ggccaaagtt gctattctaa gtggtgctga ctatttagtg
     8881 attggacgac cgattacgca atcggctaat cctatggcaa cccttcaaca gttaaaccac
     8941 gaaatcctgt cagcttactc agcaatcaat taacaactga ctcgatcaac agcgtggctc
     9001 agtccaagct accgattctt tttaatgatt tgcttctgga tggtaaatac ttcagccgat
     9061 gctggatcaa ctgcaacacg aacccaatga acattactcg caccaaaggt ttcgacacgg
     9121 gtaacatggt tgattgtttg cttcttgtta ttttgtaacg gtttatcgac gcggaaacga
     9181 tgagaatcgc catgggcaag taaaatcggc cttaagtatt gcgcagactc ctgctcaaat
     9241 acctcgatta ttccatcaaa acccagtctt gcatggcttc ctttcgcata ttcaaagttt
     9301 agatctgcat gaaatagcac aaatacgccg cgtgcatccg tatttctcgc ttcctcaaag
     9361 gtttgtttta tccaaactat cgcagcctcc gtgcgacgtt tagcttccat atcatcagct
     9421 tcagtacgat tagaaaattg cgcccaacca ttattgctcc cgacgacatg tactgtcgca
     9481 aaaacgacgt gatttttgcg ccaacgaaga ttttctacat attcggagaa gtgtggatca
     9541 cttgcttgtg ttcgtagcgt aactgttttc tgtcctaggc tatttcctgg attggcataa
     9601 aacaatcccc ttagccgcgc taagcgctcc agcgggttat atgaccctgt aaaaaagcga
     9661 tgacaatcgg tccattcatt atctccaggc gttaggataa atggaatttt gaattgctga
     9721 aaacgctgca aacgatcaat aaacatccta tctgagcaaa ctgatatgcc gttcttgata
     9781 tcacccgcat ggagcaccca tgtcaatcga gggtcagtat tgatttcatg aatcaagcga
     9841 tcaagtttat caaaatcatc cctgctatag ggcacatcac caatgagtgc aaattcaaat
     9901 ttgtcgaatg agctattgcc taattctggg gattgcgcaa tcaccagcgt agaaaaattc
     9961 agcaaaatat aggcaattat taagtgaaag cgcatcacat acattgatct gatatcgatt
    10021 cagttgatta ccatttatcc agaagctaaa taccggtttc acaagccgct agatcaccgg
    10081 tttttaaacc ttgcttaaac caactcattc tttccttaga agaaccatgc gtaaaggctt
    10141 cacgccgagg tggcagtccg gctttacgca taagctggtc atcgccaacc gcagatgccg
    10201 cttgtagtgc ttcttcgata tcgccttgct caagaaactg agttcttttt tgcgtatgat
    10261 taacccaaat gcccgctaaa caatccgctt gtaattccat cttaacctgg atcgcatttg
    10321 cgcctacctg gttggtttga ttctgaaggt tccgcacttt agttgaaacg cccgtgagcg
    10381 tctgaatatg atgccccacc tcatgagcca gcacataggc cacagccaga tcaccgcttg
    10441 cacccatacg ctgtaactca cttaagaatg ataaatccag atagatcttc tggtcacctg
    10501 gacaatagaa aggccccgac gctgcttgac ctgtcccaca agcggtcggc gtcatccccg
    10561 aataaagaac cagtttcggc gcctgatagt catagcctgc tttctggaat atttgattcc
    10621 acgtatcttc agtatctgca agtaccacac taatcaattc gaaaacctgc tgttcctcag
    10681 ggttaggttc ataattggaa ttcgatgttt caaccatgcc cgcttgctgc tgaagttctt
    10741 gtagcacgac taatggatct tgcccgaaaa taaacaccgc aatgagcgcg atgataatgc
    10801 taccaccacc aatcgcgcca ccacgcctcg catattggcc ccgtctgtct tcgatattgc
    10861 tactttgtcg ccttcctttc cacagcatat ctaactctcc ttgccctttt atgtttcttt
    10921 aggattgatg atatcagatc gacccgtgta agcgcttcca cgcatcacac tttgaagtca
    10981 ttcaagcttt caacgcttgt atccattttc tatacaattg atcagccacc ccatttagtg
    11041 cagcgatacg ttgtggaagt ctttcttgca tcgctggtgc tgtttgaact gcggggctag
    11101 ataaattttg ctcaatttcc gcggtattaa aagttgacca ggtaggcacc atctcttccg
    11161 ttacttctac atggcattgc atcgccaaat gaatatcagt ggcaaaagct tgatgcgcgc
    11221 aataagggct tgagaatagc aacgttgcct tttcaggtat ggaaaaggtt tctccatgcc
    11281 aatgaaacgc ttcaaattca gttatgtccc ccaaccaagc ttttgcaacc gggtgatcca
    11341 tcacatcgac cttaccccaa cccaactcct tgatcggtcc ttgcgttacc acgcctccca
    11401 tcgctttagt cattagctgc cctcccaagc aatggcccag gactggaata ttcagctcaa
    11461 tcgcttgttg gattaaacct agtacttgtg gaatccaagg caaatcatca ttaacactca
    11521 tcggtcctcc catcaacacg agaccgctga atgcttgcag cgttgatggg atagtttcag
    11581 cctgatctac ctttaccact ttccacggga tatgattatt atcaagaaat gtggcaaaat
    11641 aaccgggccc ttcgatagga aagaatctaa taattacaac aggtttcata atcaagcctc
    11701 tctagctttt acaaacgttt caatctttat aacacgcgct ttccattaat cttttgaaat
    11761 tctttatgtt gagtgcttta ccattgttaa tcacccatta caaaattgca aagtgtagcg
    11821 cctttagcaa atcgctttca caatgaatgt taatcaaaaa aacttttctg atctcgttca
    11881 cttagaacag ctacgtcata tcgaaaccaa taagggatca gaattatcct atgcaagtgt
    11941 tgcagatatg cgcttgcaca gtgctcggga atttagctat cgatatcttc tgaaccatct
    12001 tacgacgcgt gctaaacgct tgattcacga taatgcgttg actgaagctg tcgagcagcc
    12061 ccataaatta tttgtccgcg ccactcgaat ttgcgtcatt attgcagcca tacttggcgc
    12121 attggcggtt ggccacgcag tgggggaatc tgcgacgctg aacatttact ggcttatcac
    12181 cgtgttgtta ggctttaact tcctctccat tctgctctgg attttaggta tcagctttaa
    12241 tttacaaagc cttcggaatg gcgtcgtggc tcaactcgct tgttggttac cttatcgcaa
    12301 taaagacagt gattcgatcc gcgcattagc aactcgcgct tggtgggaaa cctgcctaac
    12361 cggatccatt ggaaaatggc ggatcagcac gttgacgcat cagttctggc ttgcttattt
    12421 aggggctgga cttaccttcc tcattttgct gatgatggcc aaacaatacg atttcatctg
    12481 gggcacaacg ttgcttcctg ataacagttt gcccaaattg acccaatttc tggggacgcc
    12541 cgttgaaatg atcgggtttc gatcccccga tacacagcaa atcgcctcga gtcgtgtcgg
    12601 cctagctatc cacgatgctg aaacacgcag tgcgtgggca agttttctat taggcatgct
    12661 cctcgtctat ggtttgttcc cgcggattat cctgttgagc atttcacttt tcatgctaag
    12721 gcttgcagaa agtcgctata agctcgattt atacctcccc tactacatca ctttgcggca
    12781 gcagctcatg tcgcatgaat ttgaagccca ggttatcgat gctgatcctc aaattgcgca
    12841 agaacgaccg gttcaaatga tcaagccggc agcgtccatc gcgccggcga atgcgctcgt
    12901 tgttggtatc gaattggagg aatccgtgca atggcctcaa acgatgaaat gcaatctcaa
    12961 tgtggtcgat caagcttcct ttgatgccgc agccgcaacc attaagcaat caaaccaacc
    13021 gttaatcatt ggtgcttcat tacagcgctt gcccgatcga ggtgtgcaac gcatgattca
    13081 tgaactggtc acgagtacct cacaacccgt ctggctttat ttgctgcgca aggataccac
    13141 tgcttccgtc aatgatgccc gcaaattggc ctggttccgc ctagccgagg cctgtactat
    13201 ccctgctgag cacgttatct atcaatgacc atgaaaaccg tgagcaaacc ttctgatcaa
    13261 gctttagatt ccctactcaa tattgcagtc gtcgggcaca ccaatacggg aaagacctcg
    13321 ttaatccgca ccatgttgcg cagcactgat tttggaaaaa tcgaagatgc agcaggcaca
    13381 acacgccacg tggaacgcgc cactatttat gccggtagtg aggctatcct caatttacac
    13441 gatacgcctg ggatcgaaga tgcgcacgga ttattgaaac gcctcaaaag caccgattcc
    13501 agaaacaaat caccagcatc ccctgcagaa atactcgaat ccttcgtcaa ttccgtatcg
    13561 gtgagcgatg cgtttgaaca ggaagccaaa gtcgttcggc aagtattacg cagtgatgtg
    13621 ttgctctaca tcatcgatgt gcgagaaccg attctcgaaa agtatttcat cgagctggaa
    13681 atactgagca aagccatgaa accgatcatc ccggtcttta attttatcgc gggacacaaa
    13741 gatgaattag ccgcatggcg aaaaaaactg gccacgatca atattcacgc ttcactcgaa
    13801 ttcgataccg ttgccttttc ctttgaagct gaaaaacggc tttatcagaa aatacaaacg
    13861 ttggtagagg cgcattatga ccgcttacaa aagcttatcg attatcgctc caaggtttgg
    13921 gatcagcttt gcttttcggc tgcaaaacgc attgtagaat tgatcgtgtc ggtcagttgc
    13981 taccggctca gcaaaatcga atccccggaa gatagccatc agtcacctgc cgccactcaa
    14041 ctacaagatt ttgtgcgcaa agctgaacaa cgctgcctgg aagatttgtt agctattttt
    14101 aatttctctg aaaaagatat cgcgctgcaa aaaatcccag ttagcaatgg gcattggcaa
    14161 cttgatattt ttgcaccggg tgtcctcaaa gcttatggac tcgatgctgg aagcgctgca
    14221 ctcaaaggtg cagccgctgg ggcaggcatt gatttgatgg tcggcggttt gagcttaggg
    14281 gcagcgagtg ccttgggcgc cttagcggga acaggatggt ctaccttcaa gcgttacggc
    14341 aaagaaatca aggccaaaat ccaaggaaca gaatggttat gcgctgacga aaacactctc
    14401 aaaattttgt atcttcgcca gcaacaatta ctgtcaaaat tgatgcagcg cggacacgct
    14461 gcttatgatc ccgatcacat tgaagaaacc gtcactgggg aattgccggc aaaatggtca
    14521 gagatcgtct cgattctcca acaaaaccca ctttggcaaa aaccttcccc acagcgcgat
    14581 cagaaccaac aattccagac actccaatcc cagcttatta atgcgttgtt ggataataaa
    14641 tagctacgta gaattgagga atatttagct cgaaaaaaac ataagggaat aacatgaaca
    14701 attccatatt atttttcatt tgtagagtag tatattgcgt catatcggat gtttaattgt
    14761 agttaggtct cacattagca caacacccac ttaccgcttc cgagttcatc gcgcagccac
    14821 cgactgctct caggattggc gcgtatggat tgaaggatct gaagtgtatc tcgccccgcg
    14881 aagcgtaggt caccagtaca aggctagctt tcattcctct ggacagtgcc aagtcggtct
    14941 ttcgtcaagt cttcgagaat ctctgattgg agatccttta tgggaaggga agagtcgctt
    15001 gtttagtgtc tggaatgttc ccacaacttt ctcaagaaac gaacgcgtca tactcgtcga
    15061 acttttcttt ccaggttcgt atttagacaa tgtcacgtca aagccaggta agtctgtcga
    15121 actactcgaa tgcccggata atcacgtggt gagtatatgc ctcctcaaat catccctaca
    15181 gtcaggcgtt tcattcaagt catccaactc atcgtttcga gaactttcgc gcctcccgtg
    15241 cacggacggt tgttcgctgt cagtacttta tcgtgtgctc ccggaaaatg atgagtaccg
    15301 caactatttg cgctatcggt actggtcaca ctatttggcc gaaccaagct cagatggtct
    15361 tactttcggt acctggggcg acatacctac atccgcagat gttagggcgc taatctggga
    15421 tggccgaact gagccaaagc aatggcacga ggtgtcggtg cgcaagctcc atgccctcgg
    15481 ccccccacaa accgatactc atggcgtacg gtgaagtcta attcaaatct tgcatgaatt
    15541 tattacatga tacatttgca aaattacata aatttcttat ttcagcgcga ttctaagctt
    15601 attgttaagt tattttacga atctcctcgc atcatttttt gccctaattg atggcttgaa
    15661 ctcaaaccaa aatgataaga aggaattaag cttcaatata agctcaaaaa tttccaacct
    15721 tagcagcgag atgctcagct aacctgagta aatcagcaca tccatcaagc ccatcaagaa
    15781 atcgttgcgg aattccggtt aatccagttt gagcgccggt gagcgcaccc gtgagaatcg
    15841 cccgtgcctg attctgacca cccccattaa tcgcatgaag gacagctgat tcaaaatcat
    15901 ccttaaatct agcagccaaa taataagccg ctggtagctg atgataaata gcgcatggca
    15961 ttccatagac aatagaaact ttccatgccg gttctatgcg aatttccgga ttctcataag
    16021 ctgcagccat ataagacggt gtaagtaaag catctggaga agcgaaacgg cctgcgcggg
    16081 gcggatcagg gtcaccaggg cggggcggct gtaaagcgtc tcgtgtgact gcatgaaaag
    16141 gtaacttgcc atccttgact agctccatga gctttcctga cagatcagta ttgagtagat
    16201 gtccttggat taaaagacct agtactgcac cataggcgac cgtcatcgaa acaacggtat
    16261 gatcaatctg agtcagcatc gtgttatcgg taatggctgc tgcaagttga ttcggctgaa
    16321 acgcgtaacg aactgcaatc gccagcgtac gttcaatcgc ctcggtagta tcggcatgac
    16381 tcccggtttg cccccacggt agtttatgct gcacccggca tcgccatgct tcacgaattg
    16441 attggctggt ataactcccg ggaccactca tcggcgtgcc atcaagcaac ggaaaaagtt
    16501 cttcgtccat tcgccggcaa aagtcatctt tattatattc accgcattca acaagcgaac
    16561 gaagagtaag caccagaata aatcccactt gagataactg ccctgcttta catccggcat
    16621 gatatctatt gggcctgggg tcagtatagt tatcaatcca gtcaccataa tcgcgacgaa
    16681 gttcgttgag atcatagtac caatgggggc ccagacctaa cgcgtcacca atgaacgcgc
    16741 ccatgattgc ccctatcgca cgatccttgg ttttattcac gaccatattt tttcctatat
    16801 tctgtcctct atctagacag tttcaaaatg ttattagctc tgtcattgtg aaatcccagg
    16861 ggccatctac tatcgttgtg tgtcaaaggg gtcaggtctt acattaacac attattgatt
    16921 ctatcgtacc agatgcaaca aaattagatg ccttcattca ctcgccctgg aaacgatagt
    16981 ctgcaaaagc gtggtggcag actggattac cggtccagcc atttcctcat gttgcatgat
    17041 ggacataggt ccttcaccga agtaaagtcc atcggacaca agaaacgtga gtcgaacgtt
    17101 atcggattca ggaggagatc ggcgtggttt cttccatggg ccgatacgcg caactacagg
    17161 ctcagatgct gctaaaagct ttttcacatg gggctcgagc tcagtggaac cttcaaaaaa
    17221 taccaattcc ccgctatggt tgatatagcg aactccgccg tcggaataag ccgcaagcgc
    17281 gtctaggcca tcggcaagag aaacttcaac gatcacgccc agtaaaactt tggttggcac
    17341 attttcccca agcaaccgca atcgggtata ggccagatac cgaatgcgac tctcttgcgt
    17401 aacatcattc gctaatgcca gcaacgcaga tcgatcagcc ggttcagcaa acagcacgat
    17461 ttgccaagta gcagcgcgct ccccttcctt gggtttgaag gctgctagat tatcgcagaa
    17521 cagtaaatta taaatct
//
LOCUS       DAOQKQ010000033        17523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_1461_length_17523_cov_5.597664, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000033 DAOQKQ010000000
VERSION     DAOQKQ010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 17523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17523
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1461_length_17523_cov_5.597664"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="PKZ49_03530"
     CDS             <1..691
                     /locus_tag="PKZ49_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HND35663.1"
                     /translation="FFRWAHLAKIVVIGMLLWLIPMSLMMQYLGWQHALTQMGWFFTK
                     AALLTFGGAYAVLPYVYQGAVDYYQWLLPYQMIDGLALGETTPGPLIMVVTFVGFVAG
                     WNLTLMGPDHLLLAGIIAGVVVTYFTFLPSFIFVLAGGPLVESTHGNLKFTAPLTAIT
                     AAVVGVIINLALFFAWHVLFPGNQQNFDFVAGLITLFAVIALFRFQVGVIPVLIYSGI
                     LGLSLTVLGLL"
     gene            887..1381
                     /locus_tag="PKZ49_03535"
     CDS             887..1381
                     /locus_tag="PKZ49_03535"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HND35664.1"
                     /translation="MRLPKILISFFLLSLYGISLLANAEQAQLKHFASGSYQQLLKQY
                     AGKPIVVMIWSIHCTICLEKMPLLSELRKNKPNINLIMIATDDVSATNQVRSILNENK
                     LDQVDNWIFADANPQKLRYEIDPKWYGEVPRTYFLDKDHQRVGISGSVPRETMETMIR
                     EIFN"
     gene            1497..2750
                     /locus_tag="PKZ49_03540"
     CDS             1497..2750
                     /locus_tag="PKZ49_03540"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HND35665.1"
                     /translation="MRFLILEKSFAQKSALKRYVFIGICLLLLIFSGYLIAESETNLP
                     EQKIYTAAAFGPDGRLWRIVPSQNFVSVDYSTDYGKSFSKQVRVNSVDIPIHLWDENP
                     PTLSVDRKGRIYALYFADDEKASTSFFSYSDNGTQFSVPIKISTEANHWYHYQTEMLI
                     DVTNRVHFIWHDVRDRAEYKKQGGGDLSIYHTATKVGEIIQFPAFDRRIAKNICSCCR
                     TAMAEDIDGSLVILARFVYPGNIRDHGLLKLSADGEVSEPWRVTFDDWKIEGCPAHGP
                     ALSISADGRYHITWFTQGEKRSGLFYAWSDDQGKTFSDPIQIGDSNRLPGRGDVLALG
                     RQVALVWKIFDGDKTYIEAMYSEDRGLHWSTPRSIAESSSQSTHPALISDGKQIFLSW
                     NSMDTGYRLIPIKQSDSNDQLSIKP"
     gene            complement(2763..3197)
                     /locus_tag="PKZ49_03545"
     CDS             complement(2763..3197)
                     /locus_tag="PKZ49_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35666.1"
                     /translation="MKQSSLKKAAIFISTFILIGQVNIASSHQLNNSLTAADAVDHFL
                     VTCGERGNHHLFVQIDDLSVVDSRQFNVLVAKDKVISSATNEDGKSSAVLRVAGGTGT
                     YHVYVSQTRGGEKAANYQLLYHCEDVDNDHLETSIFTKINQP"
     gene            3627..3863
                     /locus_tag="PKZ49_03550"
     CDS             3627..3863
                     /locus_tag="PKZ49_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HND35667.1"
                     /translation="MVAKAIFYHAGCPVCVAAEHAVANAIDRSKYQVEIVHLGTDKSR
                     VAEAEAAGVKSVPALVMDGAAFHINFGAGIEVLK"
     gene            complement(4024..4962)
                     /gene="lipA"
                     /locus_tag="PKZ49_03555"
     CDS             complement(4024..4962)
                     /gene="lipA"
                     /locus_tag="PKZ49_03555"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HND35668.1"
                     /translation="MTIRETGSAKTARNPIKINTQQSSELLRKPSWIRVRSSNSQTFN
                     EVKRMLREHKLHTVCEEASCPNIGECFGKGTATFMILGDLCTRRCPFCDVAHGRPLAP
                     DPEEPVHLAQSIAALKLKYVVITSVDRDDLRDGGAQHFADCIQQIRLHSPTTKIEILV
                     PDFRGRLSVALEKLATHLPDVLNHNLETVPRLYKQCRPGADYLHSLQLLSDFKLAFPN
                     IPTKSGLMVGLGETDDEIKQVMRDLREHHVDMLTIGQYLQPSIGHLPVMRYVAPEDFK
                     VFEHLAESMGFSHAACGPMVRSSYHADQQAHEAGIS"
     gene            complement(4979..5566)
                     /gene="lipB"
                     /locus_tag="PKZ49_03560"
     CDS             complement(4979..5566)
                     /gene="lipB"
                     /locus_tag="PKZ49_03560"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HND35669.1"
                     /translation="MGMKDYGETWQAMKTFTMNRDTDTSDEIWLLQHFPVYTQGIAGK
                     PEHLLYNNQIPVVNTDRGGQITYHGPGQLIVYTLLEFRRLGLTVRGLVTQLEKAVIDL
                     LAVYCIKAESRREAPGVYVNGAKIASLGLKIKNGFCYHGIALNVDMDMEPFAAINPCG
                     YKGLQMTQLVDLGVPDDIDVISQRIAEKLIIRLSE"
     gene            complement(5631..5900)
                     /locus_tag="PKZ49_03565"
     CDS             complement(5631..5900)
                     /locus_tag="PKZ49_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HND35670.1"
                     /translation="MDAEESSLIEYPCDFPIKVMGKSQSGFAQVVLSIVKTHAPDFDD
                     AMMEVRLSKNGTYLSLTCTILATSRTQLDNLYQELHDHPMVMLVF"
     gene            complement(5926..6792)
                     /locus_tag="PKZ49_03570"
     CDS             complement(5926..6792)
                     /locus_tag="PKZ49_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HND35671.1"
                     /translation="MIYLNGKFLPIEQAYIPVLDRGFIFGDGVYEVIPVYSRKAFRLE
                     HHLARLQDSLNGIRLSNPHTNDQWCQLITQLIDYHDSDDQYIYLHITRGVAKRDHAFP
                     VNISPTIFMMSNALLTPPKELLEKGVLAITAEDNRWGRCDIKAISLLPNVLLRQLAVD
                     AGATETILLRDGFLTEGAASNIFIVKESKLLAPPKSHLMLPGITYDVVLELVENHALP
                     YDVRPVAEQELRSANEILLTSSTKEIMPIVSLDGKLIGDGRPGPFFHKLNQLYQHYKE
                     LTMRGKQVPRDY"
     gene            complement(6799..7938)
                     /locus_tag="PKZ49_03575"
     CDS             complement(6799..7938)
                     /locus_tag="PKZ49_03575"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HND35672.1"
                     /translation="MNFILKIMKLWVMFMVSLIVTPSFAQLPELSIAAKSYTLLDYQS
                     GQTLLSQNAHERYEPASLTKLMTAYVVFSALKEKRISMDQVVPVSTTAWKMVGSRMFI
                     EPNKPVTVKELINGMIVQSGNDACVALAELIAGSEDIFAQLMNKEAERLKMTNTHFMN
                     STGLSHPNHYSSAHDLSILASAIVRDFPEYYSLYSMRDFTYNGITQSNRNRLLWLDPN
                     VDGMKTGWTDKAGYCLITSAKRDNRRLISVLMGAASESMRSKESQRLLNFGFQFFDTA
                     QLYKKHQHISSIQVWKGSQNKLNVGFDHDVYFSLPKGQSEKLKAKMEYKQPLIAPINK
                     GQEVGMVRFSIDDQQIAAYPLVALEAVDTAGIFGRAWDSVQMLFN"
     gene            8182..9033
                     /locus_tag="PKZ49_03580"
     CDS             8182..9033
                     /locus_tag="PKZ49_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HND35673.1"
                     /translation="MKHCDIDYKKIATAQPGIITKIIEINEESQNCFREIDALLRSDQ
                     AIAAFILRVANSPIYSRNQVIKTLPIAISLLGVSIIRSLVILAFSRAVFAKSRNELVC
                     KHVWHHSLLTAIASHQICYKLGNHEAGEEAFVAGLLHDIGKVLLFTHVQNEYMEALHY
                     ALEQHCPCADAEQKFLGINHRQVGEEAIKAWKLPEHFNRFVHNDLDRLAVGHIQDKVL
                     LSLAAANSIIKGRGFGANTIDPSIQKEKLIQYGLSEEFSSQLLDEAFFEELLTNDIYQ
                     FCANVHN"
     gene            9020..10669
                     /locus_tag="PKZ49_03585"
     CDS             9020..10669
                     /locus_tag="PKZ49_03585"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HND35674.1"
                     /translation="MSIINLSHEIKTILPSSLELGKDTIQITSLEQHVWSDIENKILS
                     RLNHLKCPQLIDSTSLLLHKLISNMVNTMHHRVFQQFVESDLYLGGVSGDNFFEELYQ
                     NEIEEHGDKNIASYCRRNDTQITLGFPDDLETLLTIRYPICCDSAVYLDSKLSEALGF
                     SLIKQYAGEASAHYYLAKLTRVNFEYQSSHFPGYFPLLLTRASELDKMKAIFSKLSYG
                     IVCFSSTGEILSISSAILDSLKLDSSSLSIQTFSKIIPPHFYNDVIWGLALETTEGIF
                     ENYRVRIKLPNDSGQSILFNISGYRSHDSTIQSLWQVVFLDPKSVDSLTEGSILNEAR
                     IHNITRNYVPQLVEQKARDVVRLGGKELTNEECYITVLFCDIVNFTAYVENNEKEESV
                     IHTLNLVLGRISRTVNQFEGSIDKFMGDCVMVLFKNPLNAVLAALEIQKHSLDINSLR
                     KNAGKEMLLLRIGIHWGQVIIGNIGISGRLDWTTIGDVVNTASRLEKNCQPGAVLISQ
                     ILYEVIATVEQPGIKFGDIFNIKLKGKRKELTVRYVHSDDH"
     gene            complement(10711..12156)
                     /gene="sbcB"
                     /locus_tag="PKZ49_03590"
     CDS             complement(10711..12156)
                     /gene="sbcB"
                     /locus_tag="PKZ49_03590"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008852 - exodeoxyribonuclease I activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HND35675.1"
                     /translation="MPTSLYWHDYETTGSDPHCDRPIQFAGVRTDESLQEISEPLVIY
                     CAPPRDRLPQPEACLLTGITPQFADEHGLIEFEFITKIHQALAQPNTCGVGYNSLRFD
                     DEVTRFTLYRNFYDPYAREWQQGNSRWDLIDVVRMTYALRPDGIIWPINEAGSPIFRL
                     EDLTRSNHLTHDSAHDALSDVRATIQLARLIRDRQPRLYNWLFELRDKHKVIPLLNSH
                     DHTPIVHTSRMYPAEIGCTTLVMPIGQDPRNSNSVLVYDLRYDPSAFLRMTIDELSHH
                     LFTPRSALPENSIRLPIKAIRVNKCPAIAPRSVLNDESIARIKLDLPACNQYWQIIKD
                     DKTNFMKKMVNAYSRTAFEEATDVELALYDNFFSSNDQNLIKMVRSTPPTELSNKYFQ
                     FNDKRLPELLFRFRARNWPETLTAEELECWKIHCYDYLTNKLNPNNLTISEYNETITL
                     LRGVYQENIIANDILDKIEIWGKNLVKEVQC"
     gene            complement(12161..13018)
                     /gene="metF"
                     /locus_tag="PKZ49_03595"
     CDS             complement(12161..13018)
                     /gene="metF"
                     /locus_tag="PKZ49_03595"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HND35676.1"
                     /translation="MQSQKKFPATFSFELFPPQTPEGIEKLRVARSQLAQFNPKFFSV
                     TYGAGGSTRDRTIKTVLEIQAEGRTVAPHLSCIGSTRENIYALLKKYQENGIHHLVAL
                     RGDLPSGMAQAGEFRYANELVTFIRQEFGDLFRIEVAAYPEYHPQSRSAQDDFKNFIK
                     KIEAGADSAITQYFYNADAYFHFIDQCEAANLRIPVVPGIMPINKFTQLVRFSDTCGA
                     EIPRWIRKKLEGYGDDSASITAFGLDVVTELCDRLLKAGAPGLHFYTLNSATLTTKIW
                     QRLFSNLSS"
     gene            complement(13137..14564)
                     /gene="ahcY"
                     /locus_tag="PKZ49_03600"
     CDS             complement(13137..14564)
                     /gene="ahcY"
                     /locus_tag="PKZ49_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020162970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HND35677.1"
                     /translation="MNAVTNPSGLSNFNDYKIADATLAEWGRKEIAIAETEMPGLMAV
                     REEFANKKPLAGARIAGSLHMTIQTAVLIETLVELGAEVRWASCNIFSTQDHAAAAIA
                     ANNIPVFAYKGETLSDYWDYAHRIFEWPNNTHANMILDDGGDATLLLILGSKAEKDAS
                     ILNNPTNEEEQALYSAIKSKLTQYPNWYSTRLAKIHGVTEETTTGVHRLYEMQKKNEL
                     PFPAFNVNDSVTKSKFDNLYGCRESLVDGIKRATDVMIAGKIAVVCGYGDVGKGCAQS
                     LRGLGATVWITEIDPICALQASMEGYRVVTMDDACDKADIFVTATGNIRVIGHDHMIK
                     MKNQAIVCNIGHFDSEIDIASIHQYSWENIKPQVDHVIFPTGRRIIVLAQGRLVNLGC
                     ATGHPSFVMSSSFTNQVLAQMELWQNGSNYQHEVYVLPKHLDEKVARLHLSKLGATLT
                     ELTDEQSNYLNLSKTGPYKPENYRY"
     regulatory      complement(14582..14666)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            complement(14721..15884)
                     /gene="metK"
                     /locus_tag="PKZ49_03605"
     CDS             complement(14721..15884)
                     /gene="metK"
                     /locus_tag="PKZ49_03605"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HND35678.1"
                     /translation="MSNYLFTSESVSEGHPDKVADQISDAVLDAILQQDPNARVACET
                     MCSTGLIVLSGEITTHASVDYNIIARETVKRIGYTSSDIGFDSTTCAVLTAFNKQSPD
                     IAQGVNRSKEEEMDQGAGDQGLMFGYACDETSQLMPMPIYYAHRLVERQALLRKNGRL
                     PWLRPDAKSQVSARYIDGKPHEVETIVISTQHDPDIPYAALKEAVIEEIIKPVIPETI
                     RSKQIQYLVNPTGRFVVGGPMGDCGLTGRKIIVDSYGGSAHHGGGAFSGKDPSKVDRS
                     AAYAGRYVAKNIVAAGLASRCEVQIAYAIGVAKPVSLMVDTFGTGKISDEALVQLIEQ
                     HFDLRPRAIIHQLDLLRPIYGKTAAYGHFGREEDGFTWEKTDMATQLRSAAGI"
     gene            complement(16116..17045)
                     /locus_tag="PKZ49_03610"
     CDS             complement(16116..17045)
                     /locus_tag="PKZ49_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HND35679.1"
                     /translation="MNFQQLRIISETVRQNYNLTEVANALFTSQSGVSKHIKDLEDEL
                     DIELFIRKGKRLIGLTEPGKELVKIVERILLDTKNIKRLAEQFSQHDQGQLTVATTHT
                     QAQYALPPVIREFKQLFPKVHLILHQSSPDEIVSMLLDGQADIGIATEAVKNTNELVS
                     FPFYTWHHVIIVPTGHPLQSIQPLTLEAIAEFPIITYHQGFTGRSRIDRAFEQENLTP
                     EIAMSALDADVIKTYVTLGLGVGIIASVAFMPERDFQLVKLEAPHLFEKNTTYIAIRE
                     NHYLRGYAYRFIELCVPTLSESDIRANTKPEID"
     gene            17162..>17523
                     /locus_tag="PKZ49_03615"
     CDS             17162..>17523
                     /locus_tag="PKZ49_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HND35680.1"
                     /translation="MAKTKVNNEEPLEKQLWKAADKLRKNIDAAEYKHVVLGLMFLKY
                     ISDAFGELHDQLKAGEGELAGADPEDKDEYQAENVFFVPAEARWPYLVARAKQPDISL
                     HVDAAMDAIEKENPSLKGV"
BASE COUNT         5148 a         3445 c         3583 g         5347 t
ORIGIN      
        1 cttttttcga tgggcgcatc tggctaagat tgttgtaatt ggcatgttac tttggctaat
       61 tccgatgagt ttgatgatgc aatatttagg ttggcaacat gccttgactc agatgggttg
      121 gttttttacc aaagcagctt tactaacatt tggtggcgcg tatgcggtgt tgccatatgt
      181 ttaccaaggc gcagttgatt attatcaatg gctactaccc tatcaaatga ttgatggtct
      241 tgcgttagga gagaccactc ccggaccact tatcatggta gttacttttg tggggtttgt
      301 tgcaggatgg aatctcacat taatggggcc tgatcatttg ctgctagcag gtatcattgc
      361 gggggttgtt gttacgtatt ttaccttctt accgagtttt atttttgtac tagcgggtgg
      421 accgttagtt gagtcaacac atggaaattt gaaatttacg gcgccactga cagcgattac
      481 agcagctgta gtcggcgtta ttattaactt ggcgttattt tttgcttggc atgtattatt
      541 tccaggtaat cagcaaaatt ttgattttgt ggctgggtta ataacattat tcgcagttat
      601 agcattattc cgtttccagg tcggggtcat cccagttctg atttattcag ggatactagg
      661 attatcgctt accgttctag gccttttata aaaagcttgg catgaagtac taagctaaat
      721 tcaccatggg taaatcaatc gtttaattga tcccacctgc gacaaattgt cacatgttca
      781 agaaactcag ttttatggat aattgaaatc atcaattaaa ttattttttg agtgtcgtgg
      841 tgtagttatt taatcatccc acgatcgaac attagcgaat gccatcatga gattacctaa
      901 aattcttatt tctttttttc tactgtcact ctatggaata agcttattgg cgaatgctga
      961 gcaggcgcag ctgaaacatt ttgcttctgg tagttaccag caattgctga agcaatatgc
     1021 tggcaaacca atcgtagtga tgatttggtc tattcattgc acgatctgtt tagagaaaat
     1081 gccattgttg agtgagttgc gtaaaaataa accaaacatt aatttgatca tgattgccac
     1141 tgatgatgtt tcagcgacaa atcaggttag gtccatattg aatgaaaata aactcgatca
     1201 ggtagataac tggatttttg ctgatgcaaa tccacaaaag cttcgctatg agattgatcc
     1261 gaaatggtat ggtgaagtgc cccgcactta ttttctcgat aaagatcatc agcgtgtcgg
     1321 tattagtggt tctgtaccgc gtgagacaat ggaaactatg attcgggaaa ttttcaacta
     1381 aatttctgca atagtaattt aacacgcttg gtcatgctga ttaatagcac atgtggaaat
     1441 tactggggtt tctctgattc tggttattcc gctttacata tttgatcctg aacgaattga
     1501 gattcttaat tttagaaaaa tcttttgcac aaaagagcgc cttgaaacgc tatgttttta
     1561 ttgggatatg tttattattg ctgatttttt cagggtattt aatcgccgaa tctgaaacca
     1621 atctaccaga acaaaaaatc tatacagcag cagcatttgg cccagatggc cgattatggc
     1681 gaattgtccc ttctcagaat ttcgtttcag ttgattattc tactgattat ggaaaatctt
     1741 ttagcaaaca ggttagggtt aattcagttg atatacccat acatttatgg gatgagaatc
     1801 ctccgacatt atccgtagac cgaaaaggta gaatttatgc gctttacttt gcagatgatg
     1861 agaaagcaag cactagcttc tttagttatt ctgataatgg aactcaattc agtgtgccga
     1921 ttaaaataag tacggaagcc aatcattggt atcactacca aaccgaaatg ctcatcgatg
     1981 taaccaatcg tgtgcatttc atttggcatg acgtgcgcga tcgtgcggaa tataagaaac
     2041 agggtggtgg agatttatca atttatcata cggcgaccaa ggtcggggaa atcattcaat
     2101 ttccagcctt tgatcggcgt attgcgaaaa atatctgttc ttgctgccgt accgcaatgg
     2161 cggaagatat tgatggcagc ttggtcatat tggcgcgttt tgtttatcca ggaaatatcc
     2221 gcgatcatgg tttattaaag ttatcagccg atggagaagt gagcgaaccg tggcgggtta
     2281 cgtttgatga ttggaaaatc gaaggatgtc ctgcccatgg tcctgcttta tcgatcagtg
     2341 ccgatggcag ataccacatc acctggttca cccaagggga gaaacgcagt gggctttttt
     2401 atgcgtggtc tgatgatcaa gggaaaactt tctcagatcc aatacaaatc ggtgattcca
     2461 atcgattacc tgggcgaggg gatgtattag ccttaggtag acaagtagct ttagtatgga
     2521 aaatatttga tggcgataaa acttatatag aagcgatgta ttctgaagat agaggattac
     2581 actggtcaac gccccgatca atagctgaat cgagttctca atcaacacat cctgccttaa
     2641 tttctgatgg aaagcagatt tttctatctt ggaatagtat ggatactggc tatcgcttaa
     2701 ttccgattaa gcagtctgat agtaatgacc aactatcaat aaaaccctaa atcctgattc
     2761 tgctatggtt gattaatctt agtgaagatc gatgtttcaa ggtgatcgtt atcgacatct
     2821 tcgcagtgat aaagtaattg ataattagct gctttctcgc cccctcgagt ctgactgaca
     2881 tatacatgat aagtcccagt tccgcctgct acccgaagaa cagcgcttga tttcccgtct
     2941 tcattcgttg cgcttgaaat gactttgtct tttgcaacaa gaacattgaa ttgcctgcta
     3001 tcaactacgc tgagatcatc gatttgtaca aataagtgat gattacccct ttccccacaa
     3061 gttaccagaa aatgatcgac agcatcggct gcagtaaggc tattattcaa ttgatgcgat
     3121 gaagcaatgt ttacttgtcc aatcaatatg aaagtactaa taaaaatggc agcttttttt
     3181 aacgatgatt gtttcatgat aattccttag atacacttat tatcgatgga gctagaattt
     3241 ttcctggaaa ttaggattaa ctaccataaa gcgcctgaat tcaataaaag cgtatagatt
     3301 gatagtatgt cgcaagtgat ttagaaaatt aacgcttgct cagactaaat cttatggact
     3361 ggagagtcaa tgcgacagtt tgtcacactg atgtgatgga ctgacccgat ttttccagag
     3421 gtttcttaat ttgagttaaa agaccacatt agctgacccc ttcagttggc aatattgata
     3481 tctcgctgtg tcgattacaa tcgcgtattg cccaaaccaa agctgattca ccgatacctt
     3541 tagactcatt tcgtattgga agagactcta tgttataatc tatgccaata tattgataat
     3601 ctttattttg aagaggaaaa aactttatgg tagctaaagc aattttttat cacgcagggt
     3661 gtcccgtttg tgttgcagct gagcatgcag tagcgaatgc gattgatcgt tcaaaatatc
     3721 aagttgaaat tgttcacctg ggtacagaca aatctcgtgt tgctgaagca gaagctgccg
     3781 gcgttaaatc agtacctgcg cttgttatgg acggggctgc attccatatt aactttggcg
     3841 caggaattga agttttgaaa taaataagtt taacaattaa actttttgat gttcaaaaaa
     3901 ccccgatatg gggttttttg ttttttagcg attaactcta gtttgttccg ttcaaactaa
     3961 accgtaaagt tgattgatat caacgctacc aggatcaaat cactatctac gttatctgat
     4021 aatttaactg atccctgctt catgggcctg ctgatctgcg tgataacttg atcgtaccat
     4081 agggccacaa gctgcgtgac tgaaccccat tgattctgct agatgctcaa atactttaaa
     4141 atcttctgga gccacatagc gcataactgg taaatgacct atgcttggtt gcagatattg
     4201 gcctatcgtt agcatatcaa cgtgatgttc tcgtaaatca cgcatgactt gttttatttc
     4261 atcatccgtt tcgcctaaac caaccataag tcctgacttg gttggtatgt taggaaaagc
     4321 aagtttgaaa tcactcagta actgtagtga atggagataa tcagcacctg ggcgacattg
     4381 tttgtaaagg cggggtactg tttctaagtt gtgattcaaa acatcaggaa gatgtgtggc
     4441 aagcttttct agcgctaccg ataatcgacc cctaaaatca ggaaccaaga tttcaatttt
     4501 tgttgtaggg gaatgtaacc gtatttgttg aatgcaatca gcaaaatgtt gagcaccacc
     4561 atctctcaaa tcatcccgat ctacactggt gatgactaca tatttcagtt tcagcgcagc
     4621 aatggattga gcgaggtgga ccggctcctc aggatcgggc gccaaaggtc ttccatgcgc
     4681 tacatcacag aatggacaac ggcgcgtgca cagatcaccc aaaatcataa aagtggccgt
     4741 tcctttacca aagcactcgc caatgtttgg gcatgatgct tcctcacaaa ctgtatgtag
     4801 tttgtgttct cgcaacattc gtttcacttc attaaatgtt tgactattcg aagagcgtac
     4861 ccgaatccac gacggtttac gtaatagttc gctggattgt tgtgtattga ttttaatggg
     4921 gttccgtgcc gttttggcgc ttcctgtttc tcttatagtc atggtttata attgttcctt
     4981 attcggatag tcttatgatt agtttttcag caattctctg acttataaca tcaatatcat
     5041 caggaacacc taagtcgact aattgtgtca tttgtagtcc tttatatcca caggggttaa
     5101 tggcagcaaa aggttccata tccatatcga cgtttaaagc tatgccatgg tagcaaaagc
     5161 cattcttaat ttttaatcct agcgatgcaa tttttgcacc attaacatag acacccggcg
     5221 cttcacgcct actctcggct ttgatgcaat acactgctag taaatcgata acagccttct
     5281 caagctgggt aactaaccct cttacagtta aaccgaggcg acgaaactca agtagtgtat
     5341 agacaatgag ttgtcccggt ccatggtaag taatctgtcc ccctcgatcg gtattcacta
     5401 caggaatttg gttattgtag agcaaatgtt ctggtttccc tgcaattcct tgcgtataaa
     5461 ctggaaaatg ttgaagtagc cagatctcat cggatgtatc ggtatcacga ttcattgtga
     5521 aagttttcat ggcttgccat gtttcaccat aatccttcat tcccatatat tttatttgag
     5581 taggaatgaa tgtttctgat cgcgacttag taatcgttct cacgagatta ttaaaatacc
     5641 agcattacca tgggatgatc atggagctct tggtagagat tatctaattg agtgcgtgaa
     5701 gtcgctaaaa tggtacaggt taagcttaag taagtgccgt ttttacttaa acgaacttcc
     5761 atcattgcat catcgaaatc tggcgcatgg gttttaacaa tactcaatac cacttgagca
     5821 aagccacttt gtgattttcc cattacttta attggaaaat cacaaggata ttcaatgagc
     5881 gaagactctt ctgcatccat agtactatcg tattgtattt aaatttcaat agtctctagg
     5941 aacttgctta cctcgcatgg tcagttcttt atagtgttgg tagagttgat taagtttatg
     6001 aaagaagggg cctggtctac cgtcaccgat caatttaccg tctaatgaaa caatcggcat
     6061 aatttctttc gtagaagaag ttagtagaat ttcattggcc gatcgtaact cttgctcagc
     6121 gactggcctt acgtcataag gtaaagcatg gttttctacc agttcaagca caacatcgta
     6181 ggtaatgcct ggtagcatta ggtggctctt gggaggtgct aacagcttgc tttctttaac
     6241 gataaagata ttgctcgctg caccttcagt taagaatcca tctctaagta atatggtttc
     6301 agtcgctccc gcatcaactg ccagttgacg cagtaacaca ttgggtagca atgagatagc
     6361 ttttatatcg catcggcccc aacgattatc ttcagcggta atggctaaaa cacctttctc
     6421 gagtaattct ttagggggtg ttagtagcgc attgctcatc ataaatatcg taggtgagat
     6481 attgacggga aacgcatgat ctcgttttgc tacaccccgg gtaatatgca aataaatata
     6541 ttgatcatcg ctgtcatgat aatcaatcag ttgtgtaatt aactggcacc attgatcatt
     6601 cgtatgggga ttgctcagtc gaattccatt taagctatct tgtaaacgcg ctaagtggtg
     6661 ctccaatcga aaggctttac gagaataaac tggtataact tcataaacac catcaccaaa
     6721 gataaatcct ctatccaata caggaatata ggcttgttca attggcaaaa attttccgtt
     6781 cagataaatc atcattaatt aattaaataa catttgaaca ctatcccaag cgcggccaaa
     6841 aatacctgcg gtatctactg cctctaaagc tactaatgga taagctgcaa tttgttgatc
     6901 atcgatacta aatctaacca taccaacttc ttgaccttta ttgataggcg caatcagcgg
     6961 ttgcttatat tccattttag cttttaattt ctcagattga cctttaggga gtgagaaata
     7021 aacatcatga tcaaagccca catttaattt attttgtgaa cctttccata cctgaatgga
     7081 tgaaatgtgt tgatgtttct tataaagttg tgccgtatca aaaaattgaa agccaaaatt
     7141 caataggcgc tgactttctt tgctcctcat actttctgat gcagcaccca ttaatacaga
     7201 aatcaggcga cgattatcgc gttttgctga agtaattaga caataacccg ctttatcagt
     7261 ccaccccgtt ttcataccat ctacattggg atccaaccac aataatcgat tccggtttga
     7321 ttgcgtgatt ccattgtagg tgaaatcacg catcgaatag agcgaatagt attctgggaa
     7381 atcacgaaca attgcagaag ctagaatcga taaatcgtgt gcactactat agtgattagg
     7441 atgcgatagg ccggtcgagt tcataaaatg agtatttgtc attttgagac gctcagcttc
     7501 tttattcatt aattgcgcaa agatatcttc tgaacctgca atcaattctg cgagagcaac
     7561 acaggcatcg ttacctgatt gtacgatcat cccattaatt agctctttga cagtaacagg
     7621 tttattaggc tcaataaaca ttcttgagcc aaccattttc catgcggttg ttgaaactgg
     7681 gacaacctga tccatcgaaa ttcgtttttc tttaagcgca gagaacacca catacgctgt
     7741 catcaactta gtcagtgacg ctggttcata gcgttcatgt gcattctgac taagaagcgt
     7801 ttgaccactt tgataatcta aaagtgtgta ggattttgct gcaatcgata attcaggtag
     7861 ttgcgcaaat gaaggtgtaa caatcaagct caccatgaac ataacccaaa gtttcattat
     7921 tttaagaata aaattcattt ttcttgtccg ctcaatttca aaaaaacacc ctaaacacta
     7981 ttaatactcc tgatcccaga tcaatagttg cgatataccc attattatct caacgttgct
     8041 tgataaaaaa ttacttgaca aatcccattt aaatttaacc gtgcaaatgc cgtagtttca
     8101 cttacacatt cgataagtaa cctataaggt caatatgata gcaaattaag acatcgttaa
     8161 tttgttgtta ttaaacattc tatgaaacac tgtgatattg attataagaa gattgctaca
     8221 gctcaaccgg gaattatcac aaagatcatt gaaatcaatg aggaatcaca gaattgcttt
     8281 agagagatag atgctctatt acgttctgac caagcgattg ccgcatttat ccttcgagta
     8341 gcgaattcac ctatttatag tcgaaatcaa gttatcaaaa cactaccgat tgccattagt
     8401 ttattgggtg taagtattat tcgatctcta gtcattttag cgttttcgag agctgttttt
     8461 gctaaaagta gaaatgaact cgtttgtaag catgtttggc accattcact attaaccgca
     8521 attgcaagcc atcaaatttg ttataagctc ggtaatcacg aagcggggga agaagcattc
     8581 gttgctggac tactccatga tatagggaaa gtgcttcttt ttacccatgt gcagaatgaa
     8641 tacatggagg cactgcatta tgcgcttgag cagcattgtc cttgtgctga tgcagagcaa
     8701 aaatttttag gaattaacca ccgtcaagtt ggtgaagaag caataaaagc atggaagcta
     8761 ccggaacatt ttaatcgttt tgttcataac gatctagata ggttagcagt aggccatatt
     8821 caagataaag tacttctgag tcttgcagca gctaacagca taattaaagg caggggattc
     8881 ggtgccaata cgatcgatcc atccattcaa aaagaaaaac taattcaata tggattgagt
     8941 gaggaatttt cgagtcaatt actcgatgaa gccttttttg aagagttgct cacaaacgat
     9001 atttatcagt tttgcgcaaa tgtccataat taatctttct cacgaaataa aaacgatcct
     9061 gccaagttca ctcgaactag gtaaagatac gattcaaatt acatcgctag aacaacatgt
     9121 ttggagtgat atcgaaaata aaatcttatc gcgattaaat cacctgaagt gtccacaatt
     9181 aatcgattca acatcgttat tacttcataa gctcatttcc aatatggtga atacgatgca
     9241 ccatagggta tttcaacaat tcgttgaaag cgacttatac ctgggtggag tcagtggcga
     9301 taattttttt gaagaacttt atcagaatga aatagaagaa catggtgaca aaaacatcgc
     9361 ttcctactgc agaagaaatg atacccaaat tacactcggc ttcccggatg atttggaaac
     9421 attattaacc attcgatacc ctatttgttg tgatagtgca gtatatctcg acagtaaact
     9481 ttctgaagca ttaggattct ctctaatcaa acaatatgcg ggagaagcga gtgctcatta
     9541 ctatcttgcc aagctaacaa gagtaaattt tgagtatcag agttcacatt ttccaggata
     9601 ctttccactg ctattaacac gagcatcaga gttagataaa atgaaggcta ttttctctaa
     9661 actcagctat ggtattgttt gtttctctag tacaggtgag attctatcga tttcttcagc
     9721 gattctcgat agtttaaaac tcgatagctc ttcattatca attcaaactt ttagcaaaat
     9781 tatccctccg catttttata acgatgttat ttgggggtta gcgctagaaa caacagaagg
     9841 gatttttgag aattataggg tacgaattaa gcttccaaac gattctggtc aatctattct
     9901 tttcaatatc agtggttatc gtagtcatga ttcaaccatt caatcattat ggcaagttgt
     9961 atttcttgac ccaaaatcag ttgattcact aactgaagga tcaatattaa atgaagcaag
    10021 aatccacaat attactcgaa attacgtgcc acaacttgta gagcaaaaag cgcgcgatgt
    10081 agtaagactg ggcggaaaag aattaaccaa tgaagaatgc tatatcacgg ttttattttg
    10141 cgatattgtt aactttactg cttatgttga aaacaacgaa aaagaagaat cagttataca
    10201 tacacttaat ctggttttag gaagaatatc tagaactgtc aatcaatttg agggatcaat
    10261 tgataaattc atgggagact gcgtcatggt tttattcaaa aatccattaa atgctgttct
    10321 agcagcgctc gaaatacaga agcattcact tgatataaat agtttaagaa agaatgcagg
    10381 gaaagaaatg ttgctactga gaatagggat tcattggggg caagttatca ttggtaacat
    10441 tggcatttca ggtaggcttg attggactac aattggcgat gtagtcaata ctgcgtcacg
    10501 cctagaaaag aattgccaac caggagctgt actaatcagc caaatactgt atgaagttat
    10561 tgcaacagtc gagcagcctg gtattaaatt tggtgatatt ttcaatatca aacttaaggg
    10621 aaaacgaaaa gagttaaccg ttcgctatgt tcattcagat gatcactgaa aagaatgtga
    10681 ctaaattttc tcctcattac agttttatga tcagcattgc acttctttaa ccaaattctt
    10741 tccccaaatc tcaatcttat caagtatgtc gtttgcgatg atattttctt gataaacacc
    10801 gcgaagcagg gttatcgttt cgttatattc agaaatcgtt agattatttg gattcagctt
    10861 gttagtgaga tagtcatagc aatggatttt ccaacactct aattcttcag ccgttagcgt
    10921 ttctggccaa ttgcgagctc ggaaacggaa taatagttct ggtaatcgct tatcgttaaa
    10981 ttgaaagtat ttgttgctga gttcagtagg tggagtgctt cgcaccattt ttatcaaatt
    11041 ctgatcgtta ctagaaaaaa agttgtcata aagtgcgagc tcaacatcgg tagcttcttc
    11101 aaatgctgtt ctactatagg cattcaccat ttttttcata aaatttgttt tgtcatcttt
    11161 gataatttgc caatattgat tacaagcagg taaatccagt ttaattcgcg caatactttc
    11221 atcattgagt acacttctag gagcgatggc agggcattta ttcactctta ttgctttgat
    11281 tggtaaacgg atactgttct caggtaaagc actacgggga gtaaatagat ggtgtgagag
    11341 ttcatcgatt gtcatgcgga ggaaggcgct tggatcatag cgtaaatcat atactaaaac
    11401 gctattacta tttctcgggt cttgcccaat tggcatgacc agtgttgtgc atccgatttc
    11461 agccggatac attcgagaag tatgaacaat tggcgtatga tcgtgtgaat taagtagagg
    11521 gataacttta tgtttatctc ttaattcaaa aagccagtta tatagacgtg gctgtcgatc
    11581 tcgaatcagg cgagcaagct gtatggttgc tctaacatct gataatgcat catgagcaga
    11641 atcatgcgta aggtgattag atctcgttaa atcctctagt cggaaaattg ggctaccagc
    11701 ttcattgatt ggccaaatga tcccatccgg tctaagcgca taggtcattc gtacgacatc
    11761 aatgagatcc caacgcgagt taccctgctg ccattcccgt gcataaggat catagaaatt
    11821 acgatataac gtaaagcgtg ttacttcatc atcgaatctt aacgagttgt aaccaactcc
    11881 acatgtgttc ggctgtgcta acgcttggtg aattttggta ataaattcaa attcgattaa
    11941 accgtgctca tcagcaaact gaggtgtaat tcccgttaac aaacaagctt ctggctgggg
    12001 taaccgatct cggggaggag cgcagtaaat aacgagtggt tcacttattt cttgtagtga
    12061 ttcgtcagtt cttacaccag caaattggat agggcgatca caatggggat ctgagccagt
    12121 tgtttcataa tcatgccaat atagcgatgt tggcattcca ttaagaagat aagtttgaga
    12181 acaatcgttg ccagatttta gtcgttaacg ttgctgaatt taatgtatag aaatgaaggc
    12241 caggtgcacc agccttgagt aggcgatcac ataattcagt gactacatct aatccaaacg
    12301 ctgtgattga cgcagagtca tcgccatatc cttccagttt ttttctaatc cagcggggaa
    12361 tttctgcgcc acaggtatcg gaaaatctta ctaattgagt aaatttattg attggcatga
    12421 tgcctggaac gactggaatc cttaaattag cggcttcaca ttgatcaatg aaatgaaaat
    12481 aagcatccgc attatagaaa tattgagtga tggccgaatc agcacctgct tctatttttt
    12541 tgatgaaatt tttaaaatca tcttgtgctg agcgcgattg aggatgatat tctgggtaag
    12601 ccgcaacttc tatcctgaaa agatcaccaa attcttggcg aataaacgtg acaagttcat
    12661 ttgcgtatcg gaactcacct gcttgtgcca ttcccgatgg cagatcgcct cgtaaagcta
    12721 caaggtgatg gataccgttt tcttgatatt tcttaagtaa tgcataaata ttttcgcgag
    12781 ttgagccaat acaagataaa tggggagcaa ccgtacggcc ttctgcctgg atttcaagaa
    12841 cggttttgat cgtacgatca cgggttgatc cccctgcacc ataggtaacg gagaaaaatt
    12901 ttggattgaa ttgcgcgagt tgcgaacgtg ctacacgaag tttctcgatt ccttcagggg
    12961 tttgaggagg aaataattca aaactaaatg tagcaggaaa ttttttttgt gattgcatct
    13021 cgcctaacaa tgattgaggt aagactccta aacagagtca tttatgaaaa tgtattaaag
    13081 ggtcagcata gcataattga gaatccgctg accctttagt tcgattaaat agaaaattag
    13141 taacgatagt tttcaggctt ataaggacct gttttactca gattcagata attagactgc
    13201 tcatcggtca attcagttag tgttgcgccc aatttagata aatgaagtcg agctactttt
    13261 tcatctaaat gtttcggtaa aacataaact tcatgctggt agttactgcc attttgccaa
    13321 agttccattt gagccaatac ctgattagtg aaagaactcg acattacaaa tgaagggtgt
    13381 ccagttgcac aaccaagatt gacaagccta ccttgagcta acacgataat tcttcgaccc
    13441 gtagggaaaa taacatgatc gacttgaggt ttaatatttt cccatgagta ttggtgtatt
    13501 gaagcaatat caatttctga atcaaaatga ccaatattgc agacaattgc ctgatttttc
    13561 atcttaatca tgtggtcatg accaatgacc cgaatattac cagttgcggt tacaaaaata
    13621 tccgctttgt cgcaagcatc atccatggtt accacgcgat atccttccat ggaagcttgt
    13681 agtgcgcaga ttggatcgat ttccgtaatc cataccgttg caccgagacc gcgcaatgat
    13741 tgagcacatc ctttcccaac gtcaccatat ccgcatacaa cagcaatttt cccagcaatc
    13801 attacatcgg ttgcgcgttt aatgccatcg acaagagatt ctctacaacc atataaatta
    13861 tcaaattttg atttggttac cgaatcattt acattgaaag cagggaaagg gagttcattt
    13921 tttttctgca tttcgtataa acgatgcaca ccggtagttg tttcctcggt aacgccatga
    13981 atcttagcca aacgagtcga gtaccagtta gggtactgag taagtttact tttaattgct
    14041 gaatagagtg cttgctcttc ttcattagta ggattgttga gtatcgaagc atctttctca
    14101 gctttactgc caaggatcag aagtaatgtt gcatcacccc catcgtctaa gatcatgttt
    14161 gcatgggtat tattgggcca ttcaaaaata cgatgcgcat aatcccagta atcgctgagt
    14221 gtttcgcctt tgtaggcaaa cacaggaata ttgtttgctg caatagctgc tgcagcgtga
    14281 tcttgggtag aaaagatatt gcaagaagcc cagcgaactt cggcaccaag ttcaaccaat
    14341 gtttcaatga gtaccgccgt ttgaatggtc atatgcagcg agccagcaat ccgtgctcct
    14401 gccaaaggtt ttttatttgc aaattcttca cgcactgcca taaggccagg catttcagtt
    14461 tcagcaattg cgatttcttt tctgccccat tcggccaatg tagcatcggc tattttataa
    14521 tcattaaaat tacttaggcc tgaagggtta gtcactgcgt tcatagtcaa gtctcctgaa
    14581 gaatgaacga gcgccgttga aacgatatta atcttatcaa ctgagcctaa cggggttaat
    14641 caaccgctgc agcgctcctc agttaataag ataaaagctt agattattat cattgattga
    14701 taaatgatga aaattctatt ctagataccc gcagcgctac gaagttgtgt cgccatatcg
    14761 gttttttccc aagtaaaacc atcttcttca cgaccaaaat ggccatatgc agcagtttta
    14821 ccgtaaatag ggcgcaataa atcgagttgg tgaataatcg ctctcggacg taaatcaaaa
    14881 tgttgttcga tcaattgcac taacgcttca tccgatattt taccggtacc aaaagtatcg
    14941 accataagcg aaaccggttt tgcaacgcca atcgcgtagg cgatttgaac ttcacatcga
    15001 cttgctaaac cagcagcaac gatatttttt gccacatatc ttccagcgta agctgcagaa
    15061 cgatcgactt tggaaggatc tttgccggaa aatgcaccac caccatgatg agcagagcca
    15121 ccatagctat cgacgataat tttacgtcct gttaatccac aatctcccat tgggccaccg
    15181 actacaaaac gaccggtagg attaactaag tattgaatct gtttgcttct gatggtttca
    15241 gggataacgg gtttgatgat ttcttcaatg accgcttctt taagcgctgc ataaggaata
    15301 tctggatcat gctgagttga aattacgata gtctcaactt catgcggctt gccatcaata
    15361 taccgagctg atacttgaga tttagcatca ggccttaacc agggtaaccg cccatttttt
    15421 ctcaataacg cttgtcgttc gaccaaacga tgcgcgtagt agattggcat gggcattaac
    15481 tgagaagttt catcgcaagc ataaccaaac atcaaacctt ggtcgcctgc accttgatcc
    15541 atttcttcct ctttgctgcg attgacgcct tgtgcgatat caggggattg cttattaaac
    15601 gcggtaagaa cagcacaagt cgttgaatca aaaccaatat cagaacttgt ataaccaatt
    15661 ctcttaaccg tttcacgagc aatgatattg taatcgactg aagcatgcgt agtaatttca
    15721 ccggataaaa caatcaaacc agtgctacac atagtctcgc aagcgacccg tgcattggga
    15781 tcttgttgca gtatcgcatc gaggacggcg tctgaaattt gatcagcgac tttatcagga
    15841 tgcccctctg aaacggactc ggaagtaaag agatagttgc tcatttcgta agtatactca
    15901 cctaaaatat tcaattataa caaaaattct tacattaact ttctagataa ctaggttgcc
    15961 aatacaaaca gtttcatagc gtacgactaa aaatagtaat tgcttagaat aaataagcag
    16021 ggtaaaaatg catatatgta tcaatagcac ttgttggatt tctaaatcag gtgatagcgc
    16081 ctattttttt gttaggcata acctctctct gacgtttaat caatttcagg ttttgtgtta
    16141 gctcggatat ctgattcact cagggtaggc acgcatagtt cgatgaatcg ataggcgtag
    16201 ccgcgcaagt aatgattctc acgaatcgca atatacgttg tgtttttctc aaagaggtga
    16261 ggtgcttcga gtttgactaa ttgaaaatcg cgctctggca taaaagcgac tgaagcaata
    16321 atcccaaccc caagtcctag cgtgacgtaa gttttgatta catccgcgtc gagtgccgac
    16381 atggcaattt caggtgtcag gttttcttgc tcaaaagctc gatcgatacg ggaacggcca
    16441 gtaaatccct gatgataggt gatgatcgga aattcagcga tagcctctaa tgtcaagggt
    16501 tgaatcgact gtagtggatg gccagtcggt acaatgatca catggtgcca agtgtaaaac
    16561 gggaatgaaa ctaattcgtt ggtatttttc acggcttccg tggcgattcc aatatcggcc
    16621 tgcccatcga gtaacatgga aacgatttca tctgggctag attggtgcaa aatcaaatga
    16681 accttaggaa atagttgctt aaattcacgg atgacgggcg gcaaagcata ttgggcttga
    16741 gtgtgggtgg tggcaacggt taattggccc tgatcatgct gactaaactg ttcagccagg
    16801 cgtttgatat ttttagtatc gagtagaatt cgttccacga ttttcacaag ctctttaccc
    16861 ggttcggtga gcccgatgag ccgcttacct ttacggatga ataactcaat gtccaattca
    16921 tcctctaagt ctttgatatg cttgcttaca cccgattgag aggtaaataa tgcgttagca
    16981 acctcggtca gattataatt ctggcgcacg gtctcactaa tgatacgaag ttgttgaaaa
    17041 ttcaaaaata tacctcattc atcgttggtt tcatttaagt gcggctattg tactcgcgca
    17101 ttggattagg gaacaaatcg aatgggcgta ttatgccaag caatgaggaa aacaatagat
    17161 catggccaaa actaaagtaa ataacgaaga gccattagaa aaacagctct ggaaagccgc
    17221 tgataaactg cgcaaaaata tcgacgcggc tgaatacaag catgtggtgc tcggattgat
    17281 gttcctcaaa tacatctccg atgcgttcgg cgaactccat gatcagctca aagcggggga
    17341 gggcgagctg gccggcgccg atccggaaga caaggatgaa taccaagcgg agaatgtctt
    17401 cttcgttccc gcggaagcgc gctggcccta tctggtggcg cgcgcgaagc aaccggatat
    17461 cagcctgcat gtcgacgccg cgatggacgc gattgagaag gaaaatccct cgcttaaagg
    17521 cgt
//
LOCUS       DAOQKQ010000034        17423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_1470_length_17423_cov_6.113772, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000034 DAOQKQ010000000
VERSION     DAOQKQ010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 17423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17423
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1470_length_17423_cov_6.113772"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..378)
                     /gene="rpsT"
                     /locus_tag="PKZ49_03620"
     CDS             complement(115..378)
                     /gene="rpsT"
                     /locus_tag="PKZ49_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HND35681.1"
                     /translation="MANTAQSKKRARQTIVRRGRNFRLRTRMRTAIKSVRKAIESGDK
                     ELAQSVFRESTKILDSIASKGIIHKNKAARHKSRLSHSIKVMA"
     gene            986..2086
                     /locus_tag="PKZ49_03625"
     CDS             986..2086
                     /locus_tag="PKZ49_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HND35682.1"
                     /translation="MSIQSIQLGRLKKAKLAYTPRNIALDKATSLLSGQDITPASGDL
                     VLAMVEKIGQHTSLELASGRRSTLFVGDEIIVCYGNRYAPDQFESEVPNDLLSCDLVA
                     SGGLASRMKSRHTNMKSPTKIYPIGLLTDNNGQRLNLSSIALPNLIPYAQRPYTIAVV
                     GTSMNAGKTTTAATLIRGLHNANYKVSAAKITGTGSGRDTWLMMDAGSAVTLDFTHAG
                     YPSTYLLTPEQINNVFATLTSQLSKDSPDVIVLEIADGLYQNETAKLLDSDMFAQSVD
                     SIIFAAGDAMGATSGVQWLTQKKLPIVAVSGRLTASPLAIQETVKATGLSVFNKETLS
                     SPAIANLLNIPRKKTQASSAVNWRLATMACNY"
     gene            2109..3818
                     /locus_tag="PKZ49_03630"
     CDS             2109..3818
                     /locus_tag="PKZ49_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HND35683.1"
                     /translation="MKIPVILKDQRRGLFIRLVINGLIQAGLTISTAWIVKEIFDRFL
                     LTQHLMLNFDVIGFGIALIVIAACIGWLRMVERLDAEQIGQNYIAETRILLYDYFCTL
                     APRSIQKRSRGTVLLRFVSDMTALKRWISLGLSRLTVAGVSTLTALFILAIINWQLTL
                     AVTLSLVTGGYIAYALGSRLRIAARETRRRLSYLAANLSDKVANIAVVQLFDQVGRER
                     ERIIRQNKQLHDSMIERARVAGQIHGITEFSAAVTSAIALFTGIIAVASGEATVGSVV
                     AAITVVGILMPPLRELGRVQEYWHGACVSREKLKTFLETPSFVTEISNAPALNLTSGR
                     LEFDSVTVSGGIKQLSGSIEPGQVVALVGPNGAGKSTLLQLAARLIDPDEGKILLDGQ
                     DLSQHSLKSIRRSIGMASPDLPLLRGSIEKNLRYRWRNVPEEEIIRVKTLCGIDEILA
                     QLPNGAKTKLSEGGTGLSTGQRQRIALARALLGNPLLLLLDEVDANLDAQASMIIDRI
                     IAEHHGTILLATHQLKHIKAADIVWYLEGGQLIEAGPPNKILYQNGPTVYFLGLHQQS
                     AAN"
     gene            3845..4696
                     /locus_tag="PKZ49_03635"
     CDS             3845..4696
                     /locus_tag="PKZ49_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HND35684.1"
                     /translation="MLVQSAIVMAEETTSCSGQLPRGVILSRNLPTDNQLKYYYYLPQ
                     HCTQQSNIFVTVHGISRNAEEHARKFAPYAEQYGVILIAPYFSENHFPDYQRLGRKGR
                     GKRADLALQEIIKDISKLAGINLPQKIYMFGYSGGAQFAHRYMLAYPKQVAKVVLGAP
                     GWYTFPDVSQKFPQGIQASPTLPYLTFNPSDFLKIPTCVLVGEFDKQRDNELNQSQKI
                     DRLQGKNRIERGQRWITEMTRQSRQLGLTTPYHFKLIPNSPHSFSLSMLKGGMGEKTF
                     AFLFGQN"
     gene            4750..6090
                     /locus_tag="PKZ49_03640"
     CDS             4750..6090
                     /locus_tag="PKZ49_03640"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HND35685.1"
                     /translation="MSDFWFKFPLYFIIYVSLILPPLSFAQEDISDRIEKKTKLNDTK
                     KNKKKKKKTGIFFGNAGLGYRWGKGRSEIRLSGFMQIDTRSFFDRGDDWSNSDLLLRR
                     IQPSLDVKLNSTYSFHIMPSFASAPGILDIFIDGKFSDLINLRIGKFKSPFGLERLQS
                     ATALAFNERAFPTNLSPNREIGAQLFGELLNRTTEYQIGLFSGAVDNSSGLRDSVIYS
                     NHSGIDFVSRIFSHPFYHSEHSILNGLGMGIAYSYGTQQGHASGESNLPFFLSPGQQV
                     IAAYTPGAFADGVRQRVSPQLYYHYGPLGIMSEYVISQQTITQGNFSDKVTHDAFQVQ
                     ISWVLFNGNASFRRIRPNKAIDLETMNSWGAVQLVARYAELHLDANAFKNNLLNPNLS
                     TQSANDFGVGVNWYLNRNIKLQLNYDQTHFTKGAENGVDRPDEKILFSRLQITF"
     gene            complement(6125..6613)
                     /locus_tag="PKZ49_03645"
     CDS             complement(6125..6613)
                     /locus_tag="PKZ49_03645"
                     /inference="COORDINATES: protein motif:HMM:NF025447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4262 domain-containing protein"
                     /protein_id="HND35686.1"
                     /translation="MPHPKPSNDYERKLVANIAKYGWQCTSVRSPEGDTDSVNFSYTI
                     GLNSSYGQPEFIIFGLPVETAHSILSLVADAAASGKPIDVSQPSSTLIKNYEVVFVPV
                     PRDQFSEYLCSVIWWEQGKDFAAVQIIWPDKNGLFPWHLNAPESFKMSQPILGIYTDT
                     KG"
     gene            6893..8941
                     /gene="ligA"
                     /locus_tag="PKZ49_03650"
     CDS             6893..8941
                     /gene="ligA"
                     /locus_tag="PKZ49_03650"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HND35687.1"
                     /translation="MVSPPEAVIQQIQFLRATIELHNYRYYVLNEPTIPDAEYDRLFR
                     ELQQLEQQYPELITLDSPTQRVGTAPLDEFSQFTHRTPMLSLSNAFEAAEVETFDRRL
                     CDEITVHTIEYSVEPKFDGLAVSLVYAQGLLTVGATRGDGYTGEDITHNLRTIQSIPL
                     RLPLDNPPAYLEVRGEVMILKADFIQLNQQQEKKGEKLFANSRNAAAGSLRQLDPNIT
                     AQRRLTFFAYGIGVLEPPSESLKRQSDVMTFLTSLHFPIAKEHQIVQGTIGLLKYYQW
                     MMSQREQLPYDIDGVVYKVNDLNDQIKLGFVSRAPRFAIAHKFPAQEAVTQLLDIDVQ
                     VGRTGTLTPVARLAPVFVGGVTVTNATLHNEDEIKRKQIMIGDFVIVRRAGDVIPEVV
                     AIVPEQRSVNVTAFTMPDHCPACGSRAVRLTGETIIRCTGGLFCPAQRKQAILHFASR
                     RAMDIEGLGEKLVDQLVDHAIVRTPADLYKLGIAALAQLARMAEKSANNTLHAIEHSK
                     HTTLTRFIYALGIRHVGETTAKDLARYVGQLDRLMAMNVEQLQQVHDIGPIVAESIVD
                     FFTEPHNREVIEQLRAAGVHWDENPEALEYSPSSKVQGKTFVLTGTLPTLSREAAKTL
                     IEQSGGKVTNSISTKTHYVVAGAEPGSKYHKAIELDIPILDEATLLNLLETQTIKPTS
                     "
     gene            8946..9494
                     /locus_tag="PKZ49_03655"
     CDS             8946..9494
                     /locus_tag="PKZ49_03655"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HND35688.1"
                     /translation="MKETLENAQQILSHAELICPPQSLNLAIERLSKEISDVLSKQFP
                     LVLSVMGGAIVFTGQLLPLLNFPLDFDYVHASRYNHAFQGSTLHWKIAPPEQVSGRVV
                     LVLDDVLDEGYTMTAIRQRVLAMGAKQFYCAVLADKSIGKEKPLQADFVGITLPNRFV
                     FGFGMDIKGMWRNLPAIYAIKD"
     gene            9498..10244
                     /locus_tag="PKZ49_03660"
     CDS             9498..10244
                     /locus_tag="PKZ49_03660"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HND35689.1"
                     /translation="MLAIIGGSGMTKLSCLEISRRQIVRTPYGEPSGPLMFGKINDCE
                     IVFLARHGHGHTIPPHLVNYRANLWALHQFSPQRVVAVVSVGGIRADLVPGSIVVPDQ
                     IIDYTYGRKFTFFDNTDRPITYTDFTYPYCQETRAHLLEAAKKSGELLIDGGVYAVTQ
                     GPRLETAAEVNRLENDGADMIGMTGMPEAILAKELDLPYAAIAAVANHAAGRGTSARA
                     IPLEEVYAVLEKAMISVRNILEHMVIEHGD"
     gene            10234..10767
                     /gene="def"
                     /locus_tag="PKZ49_03665"
     CDS             10234..10767
                     /gene="def"
                     /locus_tag="PKZ49_03665"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HND35690.1"
                     /translation="MAIKTVLRMGDPVLLQVAKKVEQFDTPELQTLLQDMHDTMASLN
                     GAGLAAPQIGVSLQVVIFGVDYNPRYPDAETVPYTILINPKITPLSKKMEEDWEGCLS
                     VPGMRGKVPRYTKIRYQGVDQHGNPIDRSVEGFHARVVQHECDHLIGILYPMRIRDFR
                     QFGFTDVLFPGQEIMDD"
     gene            complement(10785..11198)
                     /gene="hemJ"
                     /locus_tag="PKZ49_03670"
     CDS             complement(10785..11198)
                     /gene="hemJ"
                     /locus_tag="PKZ49_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HND35691.1"
                     /translation="MLWIKSFHIIFMVTWFAGLFYLPRLFVYHAMCTDQAGNDRFKIM
                     ERKLFYGIMTPGALLTIVFGIWLWLGYGFAGAWLHAKLALVVVLIAYHIYCRKILLDF
                     KHDRNQHSHVYYRWFNEIPVVILIAAVILVVVKPW"
     gene            11367..12611
                     /locus_tag="PKZ49_03675"
     CDS             11367..12611
                     /locus_tag="PKZ49_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35692.1"
                     /translation="MKQLQPGDHLQLKPGVYRKGLPIHHLPGNRLAPITISGPSGQQS
                     AIFIGRPGHNTVSIIDSSHITIRNLVIDGIGLPVDGIKCEGHANWAHHITLENLQIYG
                     HDHNQEIVGISTKCPAWNWIIRHNIIKNAGTGIYLGNSDGRAPFIAGLIEYNLITHTM
                     GYNLQIKHQLPRPSIPDIPIEKQHTIIRHNVFSKADQSSTGKAARPNLLVGHWPTDGN
                     GKDDQYLIYGNFLYQNATESLFQGEGRIAFYNNLLVNHQGNAIRLQPRSAQSDHISIF
                     FNTILTSEIDIFLAKPKNKSNSTYWITHNAIFTGKSATASSLTKNNLVYDYPLAGHYL
                     IQPYLMPGQMNLNLIPQDRMVPKLNINHLKNHLDWNVDFDGEISEQRFGAYTNEKRSR
                     WYPNLEIKPVLTTKQKNNLTTH"
     gene            12626..14806
                     /locus_tag="PKZ49_03680"
     CDS             12626..14806
                     /locus_tag="PKZ49_03680"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HND35693.1"
                     /translation="MKTLLVITLLIIYGSLYPGDFLTPEPDALEKFFTDLRWTTSLSD
                     MLGNVMLFVPFGLVSSLVTVTHYHSRTRLVLFFFSGFILAFALQIAQVWLPSRSAILA
                     DVIWNLVGLCIGIIIAHFVKKFTQTRIHALDTKIIIPLATLILWMISELVPLVPAIDL
                     QKFKDALKPLFLEFDLDFPTALLHMAGILLAASVLKVVTHSPLRWLTLILLTVIVGKI
                     TIISLQLDITTLVGLTAGYLFSIALLYLETSKLLAISFWVLLLAGTLERITPFVLASD
                     GTLSLIPFATMLKGSMQTSAVGLSQSLFIYTALLWLTQKMGGNFKGITIAVVIWTVFI
                     ELTQMTLLGRTADITEPLLVIFISWVFAEFQHYTNKTQKQSIPDKPYTDSSYSSPQAI
                     STIPVQGNGFLLVSIILMLGITGAIWFILRLPGIPYNLKELFLGNGNLLFIFIFVQAL
                     FWIGTGAAWVSHKVATSIRPYLILPCWTMLVSLISLILLSISVTQESIADIAGSNNLY
                     WFVMNRDIWGENWKPIFEWIGPDTVSILERIVRYTALYSPLIIFLILFTLPMFEQKKL
                     TIQQSTLLIIVTLLWLWLAKAIAFDWSSTDNLNELIARDGPMGWGGGGYLYALLLVLC
                     FNATLLAKTVNFRLSSIFSFALTIAALPLCWWLLTNGLEANVEKYGNTFSGTQFLLGP
                     DRKQALPEIELFIRWSIVQIGFVTVIGFGIRNYYQIKSLASKIK"
     gene            14913..15302
                     /locus_tag="PKZ49_03685"
     CDS             14913..15302
                     /locus_tag="PKZ49_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase system-associated protein, TIGR04073
                     family"
                     /protein_id="HND35694.1"
                     /translation="MSKPFIIFLWLTVALFFFSSQAVMAQGYSTMGSGGRTGQSYIAM
                     SGEKLATGVVNIATGIVELPKTVILTSQRDGAPYGLTVGLITGIMHSVGRTVFGALDV
                     VTFLIPTPPTVRPKYIWENFDKETTYG"
     gene            15472..15828
                     /locus_tag="PKZ49_03690"
     CDS             15472..15828
                     /locus_tag="PKZ49_03690"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HND35695.1"
                     /translation="MIFTQDKSEKRTHERVTLRTQATILLSDRKAIKVRTLDIALGGM
                     AIVADGNPNSGVVFNIQLTLPLHTDIPPLFEAKVRVAHSVLSGKEGGFKIGLQFIDLN
                     DAAESMLLQFLEQNSK"
     gene            16037..>17423
                     /gene="ubiB"
                     /locus_tag="PKZ49_03695"
     CDS             16037..>17423
                     /gene="ubiB"
                     /locus_tag="PKZ49_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HND35696.1"
                     /translation="MRFLRLLKILLIALRFGLDDILLSHAKLKILRPLTLLNPFRTRL
                     KLPRAVRLRLALEKLGPIFIKFGQMLSTRRDLLAQDFADELAKLQDQVPPFPSDLAIA
                     TLERVYEKPVNEVFLQFERQPIASASVAQVHLAVLHNGTEVAVKILRPAIAPIISHDV
                     ALMETTAWLLESIWPDGKRLKLREVVSEFARHLEDELDLVREASNCSQLRRNFLDSPL
                     LFVPEVYWDYCYPEVMVMERVKGIPISHVDTLREHNIDIPQLARMGVEIFFTQVFRDG
                     YFHADMHPGNIFVGSDGRYIAIDFGIMGSLSDEDKNYLAQNFLGFFQRDYRRVAQTHV
                     DAGWAPRNTRVDDFEAAIRAVCEPIFDKPLKEIYFGRILLRLFQASRQFNVEIQPQLV
                     LLQKTLLNIEGLGRDLDPDLDLWKTAKPFLENWMSEQIGWRGLLTHLQKEAPNWATIL
                     PEFPRLIHYHLS"
BASE COUNT         5090 a         3864 c         3401 g         5068 t
ORIGIN      
        1 gcatcattcc cctcttccat cccaggctct tcatctattc tctcctcctt ttcttgtttt
       61 aaaaagaaaa actagcacga ataatcggca ttcatatatc aagtaagacg tttattaggc
      121 cattactttt attgaatgcg ataaacgact tttatgccgt gctgctttgt ttttatggat
      181 aattccttta ctcgcaatcg agtctaatat tttcgtcgac tctctaaaaa ctgattgcgc
      241 caattcctta tcgcctgatt cgattgcttt tctaacactt ttaattgcag ttctcattct
      301 tgtccgcaat cgaaagttcc gacctcttct tacgatcgtt tgtcttgccc gcttctttga
      361 ttgtgctgta ttagccataa taaattaaat cttttttcta cctacaaata ctccgaagtc
      421 tgaatgttac gttcattcgt ttaagaatgc aaggctaagc cttattctct gaattactct
      481 tcctagatct tcgtacattt caatacgcag ccacaaaatc aattgaatga ccaaagaatc
      541 atttaattga ttattcacct tcgttggtcc aatagcgtgc ttttttaata acaatgaaac
      601 acaaaaaagt taatctcaat ttttacgcgt atcttatcca ccgcagcaac atcatgtaaa
      661 aaaatcacac aaatgtattt acgtgggaat aaattacaca tataatgttt tcatgtactg
      721 aatttcacac gcaaaaaact tcaataatcg atttgattta gcaacctgat aacactagga
      781 gatacaaaat atgagcttga aaaaaatgaa tcagaattta attatcagtt tttgctcaga
      841 tgtgcctatc acatatagca gatttcaagc tgatcaaatt agctggaatg atgccagtat
      901 attgagctag tccagcatcg ttaagtgagg agcaagtgct tctaacagtt cacataatct
      961 tatatatgag tagaggacaa tacccatgag catacagtcc attcaattag gccgccttaa
     1021 aaaagcaaaa ttagcgtata caccgcgcaa tatcgcatta gataaagcca cttctctact
     1081 atccggtcaa gacatcactc cagcatcagg tgatttagta cttgccatgg tagagaagat
     1141 tggccagcac acatcgcttg aactggcctc cggccgccgc tcaactctat ttgtgggaga
     1201 tgaaattatc gtttgttatg gcaatcgtta tgctcccgat caatttgagt ctgaagtacc
     1261 taacgatttg ttgtcttgcg atctagttgc atcaggcgga ctagcatcgc gcatgaaaag
     1321 ccgtcatacc aatatgaaaa gtccaactaa aatttaccct attggcttat taacagataa
     1381 taatggacaa cgcctcaatt tatcgagcat tgcactaccc aatctaatcc cttatgcaca
     1441 acggccttat accattgcag tcgttggaac ttcaatgaat gctggcaaaa caacgacagc
     1501 cgccaccctc attcgtggac tacataacgc aaactacaaa gtcagcgctg ctaaaatcac
     1561 tggaactggt tcgggtagag atacttggct gatgatggat gctggctccg ctgttacact
     1621 ggattttacg catgcaggat atccatcaac ttatcttttg accccagaac aaataaacaa
     1681 tgtatttgca acccttacca gtcaattaag taaagacagt ccggacgtaa tcgttcttga
     1741 aattgctgat ggcttatatc aaaacgaaac ggcaaagcta cttgattcag atatgtttgc
     1801 tcaatctgtc gatagcatca tttttgcagc aggagatgcg atgggagcca cttccggcgt
     1861 tcaatggctt acacaaaaaa aattaccaat cgttgcagtc agcggacgct taacggcttc
     1921 tccgctagcg attcaggaaa cagtaaaggc taccggtcta tctgttttta ataaagaaac
     1981 tctgagttcc cccgccattg ctaatttact taatattcca cgaaaaaaaa ctcaagcttc
     2041 ctcggctgtt aattggcgct tagctaccat ggcctgcaac tattaattat tcattcaatt
     2101 ctcgatagat gaaaatccct gtaatactca aagatcaacg tcgtgggcta tttatacgac
     2161 tggtgatcaa cggacttatt caagccggat taactatcag tactgcttgg atagttaaag
     2221 aaatatttga tcgctttctg ttaacccagc atctaatgct aaattttgat gtgatcggtt
     2281 tcggtattgc actcattgtc atcgcagcct gtataggttg gcttcgcatg gtagaacgat
     2341 tagatgcaga gcagataggg caaaactata ttgctgaaac ccgtatttta ctttatgact
     2401 atttctgtac acttgcaccc cgctctatac aaaaaaggag tcgtggtact gtattactcc
     2461 gcttcgtcag tgatatgact gccttaaagc gctggatcag tctcgggcta tcacgcctca
     2521 ctgtcgctgg cgtttcaact cttacagcat tattcattct tgcaataatc aactggcaat
     2581 taacccttgc ggtaacactt tcactagtaa caggcggtta catagcctat gcactaggaa
     2641 gtcgccttag aatagcggca cgtgaaacaa gacgccgctt atcttactta gcagctaatc
     2701 tatcagataa agttgctaat atcgctgtgg ttcagctatt tgatcaagtt ggtcgagaac
     2761 gagaacgcat cattcggcaa aataaacaac tgcatgattc aatgattgaa cgtgcacgtg
     2821 tagccggtca aattcatgga attactgaat tctcagcagc agtgacttcg gctattgcac
     2881 tatttacagg aatcattgca gttgcttcag gagaagctac tgttggttca gtcgttgctg
     2941 ctatcactgt tgttggaatt ctgatgcctc ccctcagaga attaggtcga gtacaagaat
     3001 actggcatgg tgcttgtgta tcccgcgaga aacttaaaac attcttagaa accccctctt
     3061 ttgtgaccga aatttctaat gcgcccgcac ttaatcttac ctctgggcgc cttgagtttg
     3121 attcggtcac tgtttcagga ggaataaagc aattaagcgg ttcaatagaa cccggtcaag
     3181 ttgtcgcact tgttgggccg aatggagccg gaaaatcaac attattgcaa ttggcagccc
     3241 gcttaattga tccggatgaa ggtaagattc tgctcgatgg ccaggattta tcacaacata
     3301 gcctaaaatc aattcgtcga tcaattggca tggcaagccc tgatctaccc ttattaaggg
     3361 gctctattga aaagaactta cgctatcgct ggcgtaatgt acccgaagaa gaaattatca
     3421 gagtcaaaac attatgtggt atcgatgaaa ttcttgcgca attacccaac ggcgcaaaaa
     3481 caaaactctc tgagggggga acaggtttat ctacgggcca acgtcagcga attgcccttg
     3541 ccagagcatt actaggcaac cccctactac tattattaga tgaagttgat gcaaatcttg
     3601 acgcgcaggc cagcatgatc attgatcgaa ttattgcgga gcaccatgga acaattctac
     3661 tcgcaactca ccaactcaaa catattaaag cagccgatat cgtttggtat cttgaaggtg
     3721 gtcagctaat cgaagcaggg cctccaaaca aaattttgta ccagaatggc ccgacagtat
     3781 attttcttgg gcttcatcag cagtcagccg ccaattgaaa ttactttttt ggggaatctt
     3841 attcatgtta gtgcaatctg ccatcgtaat ggctgaagaa actacatcct gttcaggtca
     3901 gctaccgcgt ggcgttattc tgtctcgcaa cctaccaacg gataatcagc tgaaatatta
     3961 ttattatctt ccccagcact gcacacaaca atcaaatatt ttcgtaactg tccatggtat
     4021 ttctcgcaat gcagaagagc atgcaagaaa atttgcaccc tatgccgaac aatatggcgt
     4081 catcttgatt gctccttatt tttcagaaaa ccactttcca gattatcaac ggttaggaag
     4141 aaaagggcgt ggcaaaagag cagatttagc tcttcaagaa atcatcaagg atataagcaa
     4201 gctcgctggt atcaatctac cccaaaaaat ctatatgttt ggttattccg gtggtgcaca
     4261 atttgcccat cgttatatgc tcgcttaccc caaacaagta gcaaaggtcg tgctaggtgc
     4321 gcctggatgg tatactttcc ctgatgtaag tcaaaaattc ccccaaggga tacaggcctc
     4381 tcccacactg ccttacctta cttttaatcc gtccgatttc ttgaaaattc ctacgtgcgt
     4441 tttagttgga gaattcgata aacaacgcga caatgaactc aatcagtcac aaaaaatcga
     4501 tcgattacaa ggtaagaacc gcattgagcg cggacaacgc tggattactg aaatgacacg
     4561 acaatcgcgt cagcttggat tgacgacacc ttatcatttc aagctaatcc caaactcacc
     4621 gcactctttc tcgctaagca tgctaaaagg tgggatgggt gaaaaaacat ttgcatttct
     4681 atttggccaa aattaaccca ttgcccgtag ccaaacttta aataacctaa gggttgattc
     4741 aaattcaaaa tgagcgactt ctggtttaaa ttcccgcttt atttcattat ctatgtttcc
     4801 ttgatactgc ctccgttaag ctttgctcaa gaagatatat ctgatcgaat cgagaaaaaa
     4861 actaaattaa acgatacaaa aaagaataaa aagaaaaaga agaaaacggg catattcttt
     4921 ggaaatgcag gcctgggcta ccgatggggt aagggaagaa gcgaaattcg cctaagcgga
     4981 ttcatgcaaa tcgatacgcg aagtttcttt gatcgtggag atgattggag caatagcgat
     5041 ttattgctac gtcgcataca accttcactc gatgtcaagc taaatagcac ttatagcttc
     5101 cacataatgc ctagttttgc atcagcgcca ggcatcttag atatcttcat cgatggcaaa
     5161 ttctcagatt taattaacct ccgtatcggt aaatttaaat cgccttttgg tctagagcgt
     5221 cttcaatctg ctaccgcatt agccttcaat gaacgcgctt tcccaaccaa tctatcacct
     5281 aatcgagaaa tcggcgcaca acttttcgga gaactcctca accgtaccac tgaatatcaa
     5341 atcggcttat ttagtggcgc tgttgataat agttcaggat tacgcgacag tgtcatctac
     5401 tccaatcata gtggcattga tttcgtgagt cgaatattct cccacccttt ttaccacagc
     5461 gaacactcaa tcttaaatgg tctgggaatg ggaattgcct atagttatgg aactcagcaa
     5521 ggtcatgcaa gtggagaatc taatctaccc ttttttcttt cacccgggca gcaagtcatc
     5581 gcggcctata caccaggtgc atttgctgat ggagttcgac aacgcgtttc accgcagctt
     5641 tattatcact atggtccgct tggcatcatg agtgaatatg tcatttctca acaaactatc
     5701 acacagggca atttcagcga taaagttacc catgacgcat tccaagttca aatatcatgg
     5761 gtattattta atggcaacgc ttcttttcgt agaatccgac ctaacaaagc aatcgatttg
     5821 gaaacaatga atagctgggg agccgtgcag ctagtagcgc gctatgcaga acttcatctt
     5881 gatgcaaatg cttttaaaaa taacctactt aatccaaatt tatcaacaca gtctgcgaat
     5941 gattttggcg taggtgttaa ctggtatcta aaccgaaata tcaaactcca attaaattat
     6001 gatcaaactc acttcacaaa aggtgcagaa aatggggttg atcgacctga tgaaaaaatt
     6061 cttttttccc gtttgcaaat aactttctga ctttgattat ctaaaaacac agttaactag
     6121 cttattaacc cttcgtatct gtgtaaattc caaggatagg ctggctcatc ttgaaagatt
     6181 caggtgcatt aagatgccag ggaaacaagc cattcttgtc aggccaaata atctgtaccg
     6241 cagcaaaatc tttaccctgt tcccaccaaa taacggaaca aagatattca gaaaattggt
     6301 ctcgcggtac tggaacaaaa acgacctcat aattcttaat cagcgtcgag cttggttgag
     6361 agacatcgat cggcttacct gaagccgctg catcggcaac tagacttaaa atactatgcg
     6421 cagtttctac tggtaatcca aaaatgataa attcaggctg tccatagctg ctattcaaac
     6481 cgatcgtata tgaaaaattt actgaatctg tatcaccttc tggtgaacga acactggtac
     6541 attgccaacc atattttgca atatttgcaa caagctttcg ttcataatca ttactaggtt
     6601 taggatgtgg catgaatcat tccctgactt tcagttcaat aagttttttg tagctgctag
     6661 ctcaatttaa gtatcctggt ctgcaataat tgcctacgct aatttggata tttcaagctg
     6721 attcttatga aatctatcga attgatttag tccgatcata ccaaattcta tttgacttat
     6781 aacctagcgc ataatgagat tcaacctact caccctttac ctctgccatg atgactaatg
     6841 gtatgatttg gattttccaa caccgtcccg tcacaactaa tcaagcttag caatggtaag
     6901 cccacctgaa gcagttattc agcaaatcca attcttacga gcgacgatcg agttacataa
     6961 ttatcgctat tatgtgctga atgaacccac aattccggat gctgagtacg atcgtctgtt
     7021 tcgtgaatta caacaactgg agcagcaata ccctgaatta atcacattgg attcacccac
     7081 tcaacgcgtc ggaactgcgc cgctggatga gttctctcaa ttcacgcatc gtacccccat
     7141 gctatccttg agcaatgcgt ttgaagcagc tgaagttgaa acatttgacc ggcggctctg
     7201 tgatgaaatt acagtgcata ccattgaata cagcgttgaa ccgaaatttg acggactagc
     7261 cgttagttta gtgtacgcgc aagggctact taccgttggt gcgacccgtg gtgatggtta
     7321 taccggcgaa gatatcacac ataacttacg cactatccag tcgataccgt tacgtttacc
     7381 cctagacaat cctcctgcat accttgaagt acggggagaa gtcatgattc tcaaagctga
     7441 ttttatacaa ctcaatcagc aacaagaaaa aaaaggggaa aaattatttg ccaactcacg
     7501 taacgccgct gctggttcac tccgacaact cgaccctaac ataaccgctc agcgccgact
     7561 cacttttttt gcatatggaa tcggtgtcct tgagccgcca tctgaatcgc ttaagcgaca
     7621 aagtgatgtc atgacttttt tgacctcact gcacttccca attgctaaag aacatcaaat
     7681 cgtacaaggt acaatcggtt tactaaaata ttaccagtgg atgatgtcgc aacgggaaca
     7741 gttaccttac gacatcgatg gtgtcgttta taaagttaat gacttaaatg atcaaatcaa
     7801 acttggcttt gtttctcgag caccccgctt tgcaattgcc cataagtttc cagcacaaga
     7861 agcagtaacg caattactcg atattgatgt gcaagttggt cgcacaggaa ccctcactcc
     7921 ggttgcccga ctagcacctg ttttcgtggg tggagtaacc gtcaccaatg caacattgca
     7981 taatgaagat gaaatcaaac gtaaacaaat catgattggt gattttgtca tcgtacgaag
     8041 agccggcgat gtcattccag aagtcgttgc aatcgttccg gaacaacggt cagttaatgt
     8101 aactgctttc accatgccag accactgtcc ggcatgcgga tcacgcgcag taagactgac
     8161 tggtgaaacg atcatacgtt gcacgggtgg cttattttgc ccagcgcaac gcaaacaagc
     8221 gattctgcat tttgcttcac gccgtgcgat ggatattgaa ggcttaggtg agaaactggt
     8281 tgatcaatta gttgatcatg ccattgttcg cactcctgct gatctttaca aattgggtat
     8341 cgctgcacta gctcaattag cgcgtatggc tgaaaaatca gcgaacaata cgctccatgc
     8401 tatagaacac agcaagcata cgaccctcac tcgatttatc tatgcgctgg gtattcgcca
     8461 tgtaggcgaa acaactgcca aggatttagc acgttatgtt ggacaattgg atcgattaat
     8521 ggcaatgaat gtcgaacaat tgcagcaagt acacgatata gggcctatcg ttgcggaaag
     8581 cattgtcgat ttttttaccg aacctcataa tcgtgaggtg atcgaacaac tccgcgccgc
     8641 aggtgtacat tgggatgaaa atccagaagc cttggaatat tcacctagtt ccaaggtgca
     8701 aggtaaaact ttcgtgctca ccggcacact ccctacccta tcacgcgaag cagcaaaaac
     8761 tttgattgag caatccggtg gcaaagttac aaacagtatt tctaccaaaa cccattacgt
     8821 ggtggctggc gctgaacctg gcagtaaata ccacaaagct attgagttag atatccctat
     8881 tcttgatgaa gctacattgc ttaatttgct agaaactcaa acgataaaac caacctcatg
     8941 atgttatgaa agaaaccctg gaaaatgcac aacagattct aagccacgcg gagctaatct
     9001 gcccacctca atcgctcaat ttggctatcg aacgtttatc taaagaaatc agtgatgtac
     9061 tatcaaaaca atttccactg gtactcagcg tgatgggggg tgctatcgtt tttacaggac
     9121 agctattacc attgcttaac tttccactcg atttcgatta tgtacatgca agtcgctata
     9181 atcatgcttt tcaaggctca accttgcact ggaaaatcgc accaccagaa caagtctcag
     9241 gtcgagtagt ccttgttttg gatgatgttc tcgatgaagg gtatacgatg accgcaattc
     9301 gccaacgcgt actcgcaatg ggagcaaaac aattttattg tgcagtattg gctgataaat
     9361 cgatcggtaa agaaaaaccg cttcaagctg attttgtcgg tattacatta ccgaatcgat
     9421 ttgtttttgg ttttggaatg gatatcaaag gaatgtggcg aaatttacct gcaatttatg
     9481 cgattaagga ttaatcaatg cttgcgataa tcggtggcag tggaatgacc aagctttctt
     9541 gcttggagat atcgcgccgt caaattgtgc gaacacccta tggcgaacca tcgggcccgc
     9601 tcatgttcgg aaaaatcaat gattgcgaaa ttgtgtttct ggctcgtcat ggacatggcc
     9661 acacgattcc tccacatctt gtaaattatc gtgctaattt atgggcgctg catcaattca
     9721 gcccccagcg agtcgtagca gtagtatctg taggcggtat tcgtgctgat ttagtaccag
     9781 gcagcattgt tgtacctgat cagatcatcg attataccta tgggcgcaaa ttcaccttct
     9841 ttgataacac ggaccgcccc attacctaca cggattttac ttatccctat tgccaagaaa
     9901 cacgcgctca tttactagaa gctgctaaaa aatcgggtga gctccttatt gatgggggcg
     9961 tttatgcagt cacacaaggt cctcgcctcg aaacagccgc tgaggtcaat cgactagaga
    10021 atgatggtgc tgatatgatt ggtatgacag gtatgcctga agccatttta gccaaagaac
    10081 tcgatctacc ctatgctgca attgctgcag ttgcaaacca tgcagctggt cgcggaacca
    10141 gtgcgcgtgc aattcccctt gaagaagttt atgctgtact ggaaaaggca atgattagtg
    10201 tccgtaatat tctagaacat atggtaattg aacatggcga ttaaaactgt tttaagaatg
    10261 ggtgatccgg tattactcca ggttgccaag aaagtagagc aatttgatac tccagaattg
    10321 caaaccctct tgcaagacat gcatgatacg atggcttcac tcaatggtgc aggcttagct
    10381 gcccctcaaa ttggggtcag cttgcaagtc gtcatttttg gcgttgacta taatccacgt
    10441 tatcctgatg cagaaactgt cccctacacg attttgatca atccaaaaat cacaccgcta
    10501 tccaagaaaa tggaagaaga ttgggaaggg tgcttaagcg ttcctgggat gcgcgggaaa
    10561 gttccgcgct ataccaaaat acgctatcaa ggtgttgatc aacatggaaa tcccattgac
    10621 cgaagtgttg aaggctttca tgcgcgggta gtgcagcacg agtgtgatca cctcattgga
    10681 attttatatc cgatgcgaat tagagatttc agacaatttg gttttacaga tgtcttattt
    10741 ccagggcagg aaataatgga tgactaacga attgatcacc ccaatcacca aggtttcact
    10801 acaacgagaa ttacagcggc gattagaatc acaacgggaa tttcattaaa ccaccgataa
    10861 tagacatgac tgtgttgatt ccgatcatgt ttgaaatcca gtaagatttt tctgcaatag
    10921 atatgatagg caatcaatac tacgaccagc gcaagtttag cgtgtagcca agcgccagcg
    10981 aaaccgtacc ccagccatag ccatattcca aaaacaatcg tcaataaagc acccggggtc
    11041 ataattccat agaacaactt gcgttccatg attttaaatc gatcattacc tgcctgatca
    11101 gtgcacatag catgataaac aaacaagcgt ggcaagtaga ataatccagc aaaccaggtg
    11161 accatgaaaa tgatatgaaa cgattttatc caaagcattg tactttcttc ccaagaatgt
    11221 ttttaattta agttttaaat aaagattata acgggtgtag cttacaattg aatttccgat
    11281 tgctctcttt cttgttgtta ttttatggta cttcagtaat agcgagtaat acttacttag
    11341 aagacccaga tagctattac gcacgattga aacaattaca acctggcgat catttgcagc
    11401 taaaaccagg cgtctaccga aaaggattac ctattcatca cttaccaggc aatcgtcttg
    11461 cgccaatcac aatttctggg cctagcggtc aacaatcggc tatttttata gggcgaccgg
    11521 gacataatac ggtaagtatt attgactcaa gtcatatcac cattcgtaat ctcgtcattg
    11581 atggaattgg gttacccgtt gatggaatca agtgtgaagg tcatgcaaat tgggcacatc
    11641 acattacctt ggaaaattta cagatttatg gccacgatca taatcaagaa attgtgggaa
    11701 tctcaactaa atgccccgcc tggaattgga ttattcgcca taatattatt aagaacgcgg
    11761 gaacaggaat ttatcttgga aattccgatg gtagagcacc ttttatcgct ggtctgattg
    11821 aatacaattt gatcacccat accatgggct ataacctcca aataaaacat caactccctc
    11881 gcccgagtat tcctgatatt cctatcgaga aacaacacac catcatccga cataatgttt
    11941 tcagcaaagc tgatcaatca agtacgggaa aagcagcgcg acctaattta ctggttggtc
    12001 actggcctac tgacggcaat gggaaagatg atcaatatct catctatggg aacttcttat
    12061 atcagaatgc tactgaatca ttattccaag gtgagggcag aatcgccttc tataataacc
    12121 tactggtaaa ccatcaaggc aatgcaattc ggttacaacc ccgctcagcg caatccgatc
    12181 atatcagtat attctttaac accatactga cctccgaaat tgatattttt ttagctaaac
    12241 ccaagaataa atcaaattcg acctattgga taacgcataa cgccattttt actggaaaat
    12301 cggctactgc atcaagtctg accaaaaaca acttagttta tgattatcca ttagcaggac
    12361 actatcttat ccaaccttac ttaatgccag gccagatgaa tttaaatctc attcctcagg
    12421 atagaatggt tcccaaactc aatatcaatc atttaaaaaa ccaccttgac tggaatgtgg
    12481 attttgatgg ggaaatcagt gaacaacgtt ttggtgctta tacaaatgaa aaaagatctc
    12541 gctggtatcc caacttggaa atcaaaccag ttttaacaac gaagcaaaaa aataacttaa
    12601 ccacacacta agccaatcaa ttcatatgaa aactctgctg gttatcactt tattgatcat
    12661 ttacggatca ctgtatccgg gggatttctt gacacctgag cccgatgctc tcgaaaaatt
    12721 tttcacagat ttgcgttgga caacatctct gagcgatatg ctgggaaacg tgatgctctt
    12781 cgttcccttt ggtctagtta gtagcttagt gactgttaca cattatcact ctcgcacacg
    12841 attagtccta ttcttttttt ctggttttat cctcgctttc gctctgcaaa ttgcgcaagt
    12901 ctggctaccg tcgcgttcag ccattctcgc tgatgttatt tggaatctgg taggcctatg
    12961 cataggaatc atcattgcac attttgtaaa aaaatttaca caaacaagaa tccatgcctt
    13021 agacactaag attattatcc ccttagccac tctcatcctc tggatgatca gtgaattggt
    13081 accacttgtg ccagccattg atctacagaa atttaaggat gcacttaaac ccctttttct
    13141 cgaatttgat cttgatttcc ctaccgcgct actccatatg gcaggaattt tattagcagc
    13201 cagtgtttta aaagtggtta ctcattcgcc actacgttgg ctaacactta tcttgttaac
    13261 agtgatcgtc ggtaaaatta ctatcattag cctacaactc gatatcacta cgctagtcgg
    13321 tttaacagcc ggctatttat tcagtatcgc cctgctatat cttgaaacat ccaaactctt
    13381 agctataagc ttctgggtac ttttattagc aggtacttta gaacgcataa ccccttttgt
    13441 gcttgcttcc gatggcacac ttagcttaat tccttttgcc actatgttaa aaggctctat
    13501 gcaaaccagt gcagtggggt tatctcaatc gctgttcatc tatactgctt tactctggct
    13561 aacccagaaa atgggcggaa actttaaagg aattacaatt gcggttgtta tttggacagt
    13621 cttcatagaa cttactcaaa tgacgttact tggacgcact gcggatatta ctgagccgtt
    13681 attggtcatc ttcatttcct gggtattcgc tgagttccag cactatacca ataaaactca
    13741 aaaacaatcg attcctgaca agccatatac tgattcgagt tactcctcac cccaagctat
    13801 cagtacaatc cctgttcagg gaaatggctt tttgctggta tcgatcatcc tgatgttagg
    13861 aataaccggt gcgatttggt ttatcctacg cttgccaggt atcccttaca accttaaaga
    13921 gttattcctt ggaaatggga acctgttgtt tatctttatt tttgttcaag cgcttttctg
    13981 gattggcact ggagcagctt gggtaagtca caaggtagcc acgagtatca ggccttacct
    14041 aatattacca tgctggacta tgttggttag ccttattagt cttatcttac tctctattag
    14101 tgtgacacaa gaaagtatcg ccgacatcgc cggctccaat aatctttatt ggtttgtgat
    14161 gaaccgagat atctggggag aaaattggaa acctattttt gaatggattg gccctgatac
    14221 ggtctcgatt ctcgaacgaa tcgttagata tactgcttta tattctcctc tcattatttt
    14281 tttaattttg ttcacattgc ctatgtttga gcaaaaaaaa ttaaccattc aacaatcaac
    14341 ccttcttatc atcgttacat tactctggct ctggctggct aaagctatcg cctttgactg
    14401 gtcttcaaca gataatctga atgaattgat tgctcgagat ggtcccatgg gatggggagg
    14461 tggaggttat ctctatgcct tgttgttggt gctttgcttt aacgccacac tactcgcaaa
    14521 aaccgttaat tttcgattaa gcagtatatt ctcgtttgca ctcactattg ccgcattacc
    14581 cctctgttgg tggctattaa ccaacggttt agaagctaac gttgaaaaat atggaaatac
    14641 cttctcagga actcagtttt tactaggacc agatcggaaa caagcgcttc ctgaaattga
    14701 actgtttatt cgttggagta tcgttcaaat tggctttgtg acggtaattg gttttggcat
    14761 tcgcaactat tatcaaatta aatcactggc ttctaagata aaatgagcga aattgcatcc
    14821 ttaattcaat tctatctttc agcttaatat gaattagaat tcacgcaata gtccaagctg
    14881 ttattaattc cttttactta caggagaaag taatgagtaa gccttttata atattcttat
    14941 ggttaacagt agcactgttc tttttttcat ctcaagcggt aatggcacag ggttactcaa
    15001 ccatgggcag cggaggtaga acagggcaaa gctacatcgc catgtcgggc gagaagctgg
    15061 cgactggtgt cgttaatatc gcaaccggga ttgtagagtt acctaaaacc gttatcttaa
    15121 ccagtcaacg cgatggagca ccctacggtt taaccgttgg ccttatcact ggcattatgc
    15181 atagcgttgg ccgcactgta ttcggcgcat tggatgttgt gacttttctg attccaaccc
    15241 ctccaaccgt tcgtccaaaa tacatttggg aaaactttga taaagaaacg acctacggtt
    15301 aattagtggc tcgatcagtg tggatcaata agcatttatc aatcgcattc atgcatattg
    15361 atccactttt aaaatgaccc gtctgcactt tacgtttagc aagaaacata cactggattt
    15421 cttccatcaa aaaaataaaa catcctagat gtagctaata agcgatgaag catgatcttc
    15481 actcaagata aatccgagaa acgtacacac gaaagagtaa ccctgcgaac tcaagcaacg
    15541 atccttttgt cggacagaaa agcaataaag gttcgtacgc ttgatattgc attaggtggc
    15601 atggcaattg tggcggatgg taatcctaac tctggcgttg tttttaatat tcaacttacg
    15661 ctcccactgc atacagatat tcctcctcta tttgaagcaa aagtaagggt agcgcatagt
    15721 gtcttatcag gtaaagaagg aggattcaag attggcttac aatttatcga cctcaatgat
    15781 gctgccgagt caatgctatt gcagtttctg gagcaaaatt caaagtaaag gtgcctctta
    15841 acggtatttc caaccagatt ggaatagcca tccaatcatc ggcactaatc aaagcacatt
    15901 actaacaaac agcacttttt ttgttatgcg ctatgatcac aaaatttcat ttcagacaaa
    15961 ttgcacagaa acaagttaaa attggcagca atgcaatagt cgatgactcg tgatttacca
    16021 acctaattac aaataaatgc gttttctacg cctcctaaaa atcttattga ttgccctccg
    16081 cttcggctta gatgatattt tactaagcca tgcaaaactt aagattttaa gacctcttac
    16141 actgctgaat ccttttcgta cacgtttaaa gttgccgcgt gctgtacggc ttcgtttggc
    16201 actcgagaag ctagggccga ttttcatcaa gttcggtcaa atgctttcaa cacggcgtga
    16261 tctcttagcg caagattttg ctgatgagtt agctaaatta caagatcagg tccctccttt
    16321 tccttctgat ttggcaatcg ccacattaga gcgtgtttat gaaaaacctg tcaatgaagt
    16381 atttttacaa tttgagcgac aacccattgc tagcgcttcg gttgctcaag ttcaccttgc
    16441 cgttctgcac aatggaaccg aggtagcagt caaaattcta aggcccgcta ttgcaccgat
    16501 tatttcgcat gatgttgctt tgatggaaac aaccgcttgg ctattagaat caatttggcc
    16561 ggatggaaaa cgcctaaaat tacgtgaagt cgtgtcggaa tttgcaagac accttgaaga
    16621 tgaacttgat ctcgttcgtg aagcgtctaa ctgtagtcag cttcgtcgca attttctcga
    16681 ttcaccttta ttattcgttc cagaagtcta ttgggattat tgttatcctg aagtaatggt
    16741 aatggaacgg gttaaaggaa ttccaattag tcatgttgat accttgcgtg aacataatat
    16801 cgatatccct caacttgctc gtatgggcgt tgaaatattc tttacacaag tattccgaga
    16861 tggttatttc catgctgata tgcaccctgg taatatcttt gtaggttcgg atgggcgtta
    16921 tattgcaatc gattttggaa ttatgggatc acttagcgat gaggataaaa actacttagc
    16981 gcaaaatttc ttaggcttct ttcagcgtga ctatcggcgg gttgcgcaaa cccatgtcga
    17041 tgccggatgg gcccccagga atacccgtgt tgacgacttc gaagctgcta tccgtgctgt
    17101 ctgtgagccg atttttgaca aaccactcaa agaaatttat tttggaagaa tcttattgcg
    17161 cctattccag gcatctcgcc aatttaatgt agaaattcaa cctcaactcg ttttgttaca
    17221 aaaaacacta ctcaacattg aaggtctggg gcgagatctt gatcctgatc ttgatttatg
    17281 gaaaacagcc aagcctttct tggaaaactg gatgtcagag caaattggtt ggcgcggtct
    17341 gctcacccat ttacaaaaag aagcccctaa ttgggccaca attcttcctg aatttccccg
    17401 actcattcat taccacctga gtc
//
LOCUS       DAOQKQ010000035        17376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_1476_length_17376_cov_5.318746, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000035 DAOQKQ010000000
VERSION     DAOQKQ010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 17376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17376
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1476_length_17376_cov_5.318746"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="PKZ49_03700"
     CDS             <1..725
                     /locus_tag="PKZ49_03700"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HND35697.1"
                     /translation="MELRSLMLISNLDSLSIRLSLLLNDCLRLCTSILSGGLLLLPFL
                     ATEVLAQQSARNVEGCSIRRGTFCVDMSLNGADLQNTNLANSQFTRSILSGANLTKAV
                     LDEANLSDAQLRGTILNQASLIKTNARRARFNDAQLVGANLRSAILQNADFSGANLTD
                     TVLTSADLTNAKLVGASLNNANIQRANLRSAHLQNASLVNANLQGVDFTGADLSNTDL
                     SGANLDQAIFTMARTKGCKGCP"
     gene            855..1181
                     /locus_tag="PKZ49_03705"
     CDS             855..1181
                     /locus_tag="PKZ49_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HND35698.1"
                     /translation="MMLLPGQRMKEEVKQRLQRTETWVQGFFILFFVFVSGVVESLII
                     MIMFFQYVSILVTGKTNTHLLRLGKYLAAYVYQIVLYLTFNTGQRPFPFTIWPTEASD
                     KEGSKT"
     gene            1579..2463
                     /locus_tag="PKZ49_03710"
     CDS             1579..2463
                     /locus_tag="PKZ49_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HND35699.1"
                     /translation="MRKLILLTILSLVVTAVSANQSSSELFLDDLPVIDKDPNELFAL
                     AVKYEHAEGFPEDKTKAAELYCEAARLGSADAQFALGWMYANGRGVERDDSIAAQLFE
                     MAAEQGHHYAQKMIQYTPAMLPVSLPACLIPSIEIFDDEEDSDYTYPEGPVLHLVHKL
                     APDYQLDPKLVLALISIESAFKVKAISPKNAQGLMQLIPATAQRFQVKDTFDPEENIK
                     GGMAYLRWLLAFFKGDVTLAVAAYNAGEGAVEKYRGVPPYRETLNYVEKIRGRYKKVT
                     HPYQAEVVSRASILVSAR"
     gene            2528..3292
                     /locus_tag="PKZ49_03715"
     CDS             2528..3292
                     /locus_tag="PKZ49_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HND35700.1"
                     /translation="MVKPVLLVALVILLLTHYLTEHVNASWRTASRESVGLAPSPEQT
                     PEALIQVYGARAYGWRGYFGVHTWIAVKPSNANAYTVYEVIGWYLRRGGSVVAIHEQI
                     PDRRWFGNMPQLLIEKRGEGVDALIERVEQAAYQYPYHYEYWIWPGPNSNTFTAWISR
                     AVPELDLDLPPTAIGKDFLGKEWVATAPSGHGWQWSMFGLLGVTVSRIEGFELNILGL
                     TFGIDPDPFAIKLPLIGRIDIPILDLLSASSTIQEK"
     gene            complement(3303..4478)
                     /gene="clsB"
                     /locus_tag="PKZ49_03720"
     CDS             complement(3303..4478)
                     /gene="clsB"
                     /locus_tag="PKZ49_03720"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HND35701.1"
                     /translation="MNNPDFIDHNKIILLNNGSQYFPALVSAIEQAKYEIHLQTYIFK
                     YDATGIIISEALTRAAKRGVAVHLLMDGFGSLDFPNKIIEKLITEGVQVLIYRKELLT
                     FKFLKHRLRRMHRKVVVIDAQIAFIGGINIIDDHDPRASQPLPRFDHAVRIEGPLLAN
                     IYPVVKRLWMLVAWVHFKKRWIKKLPELKPQLKSSGNQRAALVIRDNIRHRRDIEHAY
                     LKAIATAEEEIIIANAYFLPGRNFRKALIHAARRGVRVILLLQGKIEYRIQHYASRAL
                     YGNLLEAGIKIYEYHKSYLHTKVAVIDKYWSTVGSSNIDPFSLLLAREANIIVTDHAF
                     SSELRANLQQALHESNPVSARYWKKRAWLDRLLNWISYYTLRFLQGTLGYGQKSTRI"
     gene            complement(4475..5227)
                     /locus_tag="PKZ49_03725"
     CDS             complement(4475..5227)
                     /locus_tag="PKZ49_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HND35702.1"
                     /translation="MTNTLHIATYNIHKGFSLFNQRIVLHEMREKLRTLGTDIIFLQE
                     VVGAHRKHAMRFSNWPSNPQYEFLADSVWRDFAYGKNAVYDEGHHGNAILSRFPIIRW
                     ENIDISAHRFEQRGLLHCEMDIPGWQEPLHCICVHLGLFKRGQAMQLRAIEKRLTEII
                     PPTAPLVIAGDFNDWHGSAKRILTESMKLVEVFEQTHGRTARSFPSLFPLLRLDRIYV
                     RGFQVKNAVVHQSPTWARISDHAALTANIVRR"
     gene            complement(5298..6398)
                     /gene="nadA"
                     /locus_tag="PKZ49_03730"
     CDS             complement(5298..6398)
                     /gene="nadA"
                     /locus_tag="PKZ49_03730"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HND35703.1"
                     /translation="MQANTIDFENYELLQDEICAQRIVAAKAKLGKKAVILAHHYQRA
                     DVYCHADLTGDSLKLSYLAAQTDADYLVFCGVHFMAEVADILSSPQQIAILPDLSAGC
                     SMADMANLSKVERAWRELSEILNPDEKITPVTYINSSADLKAFCAEHGGIVCTSSNAT
                     KVLQWSFEQREKVLFFPDQHLGRWSGYKMNIPLDEMLIWDFDEPMGGLSEAQIKKAKI
                     LLWKGHCSVHQMFQPQHIIRFRNQYPDGIVISHPECSFEVCKASDYVGSTEYIIKTIR
                     EAAPGTRWLVGTELNLVGRIAEEFKAQGKIVQFMSPMVCMCSTMARIDPQHLAWSLEN
                     LVAGKIVNQIKVPDQEAQLAKLALERMLAISK"
     gene            complement(6504..8711)
                     /gene="glgB"
                     /locus_tag="PKZ49_03735"
     CDS             complement(6504..8711)
                     /gene="glgB"
                     /locus_tag="PKZ49_03735"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HND35704.1"
                     /translation="MKSKQLSTVSYPLQHELLTARLHDPFAYLGAHPVSNETVVRAFL
                     PHAEKVWLQTEAGFTEMACAHVDGLFEWRGKDSIPKCYRIRMRKKNDAAPENLHEFYD
                     PYSFPLQISDLDLYLFNEGKLHQAYRMLGAHHLTINGIAGIRFSVWAPNAERVSVVGD
                     HNGWDGRVHPMAVHHQSGVWELFIPGFPGHGIYKYEIRNRHSGEILLKTDPFANYHEI
                     RPNNAAYTPTHSSFTWSDDLWMNQRKHWDWLHAPLNLYEIHVGSWKRHPDGKFFNYRE
                     LAHHLIPYLLEMGYTHVELLPISEHPLDESWGYQTTGYFAVTSRYGSPEDFKFFINAC
                     HQANIGVILDWVPAHFPQDSFSLARFDGTALYEHEDPRLGFHHDWGTFIFNYGRNEVK
                     SFLLSSAHYWLNNFHLDGLRVDAVASMLYLDYSRQHGEWLPNKYGGRENLEAIDFLRE
                     LNIMVHDAFPGALTFAEESTAWPAVSRPTYVGGLGFSMKWNMGWMNDTLSYIRQDPIY
                     RRYHHNQLTFNQLYAYSENFVLPFSHDEVVHGKGSLLGKMPGDAWQKFANLRLLLTYQ
                     MTCPGKKLNFMGNEFAQAREWQVNQQLDWNLLDYKLHRGVQLAIHDLNHLYLETPALH
                     QLDFEQAGFCWADCNDADQSVISYLRYAQDGSFSLIVLNFTPVPRENYRIGVPARGIY
                     HEVFNSDSTYYGGGNVGNPKQIFSVAEPWLEYQDSVIITLPPLAGIILALTPA"
     gene            8915..10162
                     /gene="glgC"
                     /locus_tag="PKZ49_03740"
     CDS             8915..10162
                     /gene="glgC"
                     /locus_tag="PKZ49_03740"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HND35705.1"
                     /translation="MATRNTLALILAGGRGSRLHNMTDWRAKPAVPFGGKFRIIDFPL
                     SNCVNSGVRRIGVVTQYKAQSLIRHIQRGWSFLDGRFKEFVELLPAQQRTEETWYQGT
                     ADAVFQNLDILRNHDPSYVLILGGDHIYKMDYSKLLDEHIQKGADMSIACLEVSFEEA
                     RAFGVMAVDEQWRITSFAEKPENPTAIPGQPDRALASMGIYVFNSQFLFEQLIRDHDD
                     NASTHDFGKDIIPYLVNSQYRVYAHRFTNSCVNMASGIPYWRDVGTIDAYWEANIDLT
                     TVTPDLNLYDEDWPIWTYQEQLPPAKFVFDDDNRRGQALDSMVSGGCIISGATIRRSL
                     LFSNVQVRSFGIIEDSVILPNVDIGRHARLKNVVVEKNCIIPEGLRVGFDAEEDGKRF
                     HVTKKGITLITPEMLGQGIHELR"
     gene            10225..11907
                     /locus_tag="PKZ49_03745"
     CDS             10225..11907
                     /locus_tag="PKZ49_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HND35706.1"
                     /translation="MWHMHQPDYRDHETGEFLLPWVYLHAIKDYTDMAYHLEKHPRVK
                     AVINFVPILLEQLEDYSDQFLQETLRDPLLRLLITPDLNLISLEDRMFILNQCFQSNH
                     DTMLKPYRAYQHLRDIYETLIGNGDAEMSYVSGQYLADLLTWYHLAWTGESVRKNCEL
                     VGKLMQQGKSFTYSDRKQLFELIGTLIQDLISRYRKLAVSGQIELSTTPHYHPLSPLL
                     IDFNSAYDSLPDAVLPSSSDYPGGRDRVAYHLELAKESHKQRFHSYPFGVWPAEGGIS
                     KDFVEILAASELKWTASGEGVLAQSLRKSYPDRQLPERSEYLYRAYKVLTNKEDVICF
                     FRDDKLSDMIGFEYAKWYGRDAAENFVHSLERIHHEVKDAEDPVVTIILDGENAWESY
                     PYNGYYFLNDLYEMIGNHLAIKTSTFRDYLVHLKESDTEAVFLPNLAAGSWVYGNFST
                     WIGSHDKNLAWDILCKAKKSYDAVLKIGNLTDEEQAAAKRQLASCESSDWFWWFGDYN
                     SSQAVASFDRLFRKNLANLYSLLKQPIPSELSEPISYGNSNARMIETMRRSN"
     gene            12015..14042
                     /locus_tag="PKZ49_03750"
     CDS             12015..14042
                     /locus_tag="PKZ49_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1926 domain-containing protein"
                     /protein_id="HND35707.1"
                     /translation="MSKPVSLLFGVHAHQPVGNFPEVLEDAHIRCYKPFLHVVHRYPD
                     FKFAVHFSGWLLDYLFDHHPEDMKLLKAMVKRGQVELFSAGDTEPVLATIPNRDRIGQ
                     LETFSKKLNKKLGQRPQGAWLTERVWESTVIPALVDCGISYVIVDDYHFLCAGKTKAE
                     LNGYFTTEEDNQRLDLFPISEALRYKLPFSPASDAIAYIESLSEHQAASQRVAAIYFD
                     DIEKFGIWPETYQWVYEKGWLEQFIQGVLASPQIMTSHYRDYHSSEKSRGIIYLPTTS
                     YIEMNEWTLPADLANRYADLVQQSKVSGSYEHNKPFLRGGIWKNFFSRYPESNWMHKR
                     MLSLSQRFASLPVKQQSATMREKLYASQANDAYWHGLFGGLYLPHLRRAVFNNIVELE
                     GMLDLIAPRPTIYIEDTDLDGVEEIYLNNGILQVISKLDGAASICEFDAYKLKHNFGD
                     TLRRQEEHYYHKIKYDMPNLQQSGSGIASAHDRLSFKHQISSADLEGGRAAQALFVES
                     IDDHLVMYQFESKHDHGIKFKADFDGYKINKKIELIENQLLITYTSKSRVEGYLKTEF
                     NLAMPSCDGVGGKYIRDDLVLGGFGQELELMDFISIFLEDSTLKGRLLLSVSNESRFS
                     SKPFYSVSQSEGGFEKIMQAIKLVLVWPCIPERLTISLTVVELGGVVEVYS"
     gene            14226..14603
                     /gene="rpsL"
                     /locus_tag="PKZ49_03755"
     CDS             14226..14603
                     /gene="rpsL"
                     /locus_tag="PKZ49_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HND35708.1"
                     /translation="MPTTSQLIRKPRQDKRVKSKVPALESCPQKRGVCTRVYTTTPKK
                     PNSALRKVARVRLTNGFEVSSYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQGRSKYGSKKPKVS"
     gene            14649..15119
                     /gene="rpsG"
                     /locus_tag="PKZ49_03760"
     CDS             14649..15119
                     /gene="rpsG"
                     /locus_tag="PKZ49_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HND35709.1"
                     /translation="MSRRREIPKREILPDPKYSNIEVAKFVNVLMTRGKKSVAERIIY
                     GALSQVEKKTGKNPVEVFTIALGNIRPIVEVKSRRVGGANYQVPVEVRSVRRNALAMR
                     WLRDAARKRNEKSMDLRLAVELIEASEGRGGAIKKREEVHRMAESNKAFSHYRF"
     gene            15185..17278
                     /gene="fusA"
                     /locus_tag="PKZ49_03765"
     CDS             15185..17278
                     /gene="fusA"
                     /locus_tag="PKZ49_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HND35710.1"
                     /translation="MSRKTPIERYRNIGIMAHIDAGKTTTTERVLFYTGVSHKLGEVH
                     DGAATMDWMEQEQERGITITSAATTCFWSGMAKNYPEHRINIIDTPGHVDFTIEVERS
                     LRVLDGACTVFCAVGGVQPQTETVWRQANKYKVPRLAFVNKMDRSGANFNRVQEQIKS
                     RLKALPVPIQLPIGAEDKFEGIVDLIKLKAIYWDETSQGMRYDERDVPEGMLDSVKAA
                     RESMIESAAEASEELMNKYLESGELAAEDIKNGIRIRTLANEIVPMLCGSAFKNKGVQ
                     AMLDAVIDFLPSPLDKPAIQGLKSNGEKDERHASDEEPFSSLAFKIATDPYVGQLIFF
                     RVYSGVVNSGDTVYNSVKQKKERVGRLLQMHANQRDEIKEVRAGDIAAAVGLKEVITG
                     DTLCDTEHIITLERMDFPEPVIHVAVEPKTKNDQEKMGIALNRLAQEDPSFRVRTDEE
                     SGQTIISGMGELHLEIIVDRMKREFSVEANVGAPQVAYREAIKKKVEIEGKFIKQSGG
                     RGQYGHVWLRMEPNEAGKGFQFIDEIKGGVVPREYIPAVEKGLIDSLTNGVLAGYPVV
                     DVKIALFDGSYHDVDSNENAFKMAASIAFKDGMKKATPILLEPIMSVEVETPPDFMGN
                     VVGDLSSRRGMIQGMDDTVGFKIIKAEVPLAEMFGYSTVLRSATQGRATYTMEFKHYS
                     EAPKSVAEAIINKNK"
BASE COUNT         4952 a         3151 c         4144 g         5129 t
ORIGIN      
        1 ttatggagtt gcgctcgctt atgttaatta gcaatctcga ttcattatcg attcgcctaa
       61 gtttattgct gaatgattgt ttgcgcttat gtactagcat ccttagtggt gggctgttgc
      121 tactcccatt tctcgctacg gaggtgcttg cgcaacaaag cgcgcgcaat gttgaagggt
      181 gtagtattcg acgtggtacg ttttgcgttg atatgagtct aaatggcgcc gatctgcaga
      241 ataccaatct ggcgaattca caatttaccc gttccatatt atctggcgcc aatctgacca
      301 aggcggtgct cgatgaagca aacttatctg atgcgcaatt acgcggaacg attctaaatc
      361 aagcttcgct aatcaaaacg aatgcaagaa gagcacgatt taatgatgcg caattggtgg
      421 gagcaaatct aagaagtgcg attctacaaa atgctgattt tagtggcgca aatttaacgg
      481 ataccgttct aacgagtgcg gatttgacta acgccaaact ggttggggca tcattaaata
      541 atgcgaatat tcagcgtgcg aatctgcgtt ctgcccattt acaaaacgca tccttggtga
      601 atgccaattt gcaaggcgtt gattttactg gcgctgattt gagtaatacc gatctgagtg
      661 gtgccaatct cgatcaagct atctttacca tggcgcgtac taagggatgt aagggctgtc
      721 cctaagatct tattgagggg ttgattggct agcactagtt tggttgtttt ttgttcacac
      781 tgatctataa agagttttgc taatctttat aaattcattt actgtgtagt ctactctcaa
      841 cgcatagtac ggatatgatg ctacttcctg ggcaaagaat gaaagaagaa gtgaagcaac
      901 gattgcagag aacagagacc tgggtacaag gtttttttat cttgtttttt gtttttgttt
      961 ctggtgttgt tgaatctttg attatcatga ttatgttttt tcagtacgtt tccattttgg
     1021 taacggggaa aacaaacacg catttgctca gattggggaa gtatctcgca gcttacgtgt
     1081 atcagatcgt tttgtatttg acgtttaaca ccgggcaacg tccttttccc tttactatct
     1141 ggccaactga ggctagcgat aaggaaggaa gcaagaccta acaattcttc gttgctgtga
     1201 tttttcacag cgatatcttt ggtaatttgt cgtttaatga caaatcctat gtggtttggt
     1261 ttaacataaa gcaagtgtcg gaaaatttat cacttttttt aagttggctc cgggtcgtgc
     1321 tgttatcttg ctgatttaaa agcattcatt atcgctggca tagtagctgc tttagtaatg
     1381 gcaaagaaag tattagtaac aaaaccaaac agcccaggtt ttgctgttta gtagccgacg
     1441 cagtaaaatc aacattcgat cgtattggac tttttggtat ttatccattg tctactttta
     1501 gtaagagcgg tcagattctt tcgattaagt tcaactgtgc aggcgtttat tcggaatttg
     1561 tcagaaaaat cattttttat gcgaaaacta atattactca ctattttgtc attagttgtt
     1621 accgcagttt ctgccaacca aagttcatca gagctatttt tagatgactt gccggttatc
     1681 gataaagatc ctaatgaatt atttgcgcta gcagttaagt atgaacatgc tgaaggtttt
     1741 cctgaagata aaaccaaagc agcagagtta tattgcgaag cggcacgctt aggatcggca
     1801 gacgcgcaat ttgcactggg ttggatgtac gcgaatgggc ggggtgtaga acgcgatgat
     1861 agtatcgccg cacaattgtt tgagatggcc gcagaacagg gacatcatta tgcacagaaa
     1921 atgattcagt atacaccggc catgttaccg gtttcgcttc ccgcttgctt gattccgagc
     1981 attgaaattt ttgatgatga agaagacagt gattacacct atccagaggg ccctgtactt
     2041 catttggttc ataaattagc gcctgattat caattagatc ctaaactcgt tttggcactt
     2101 atctcgattg agtccgcatt caaggttaaa gcgatttctc ctaagaatgc gcaaggattg
     2161 atgcaattga taccagcgac ggcacagcgc tttcaagtaa aggatacatt tgatccagag
     2221 gaaaatatca agggcgggat ggcctactta cgctggctgt tagccttctt caaaggtgac
     2281 gtgacgcttg cagtcgcggc ctataatgct ggggaaggcg cggttgagaa gtaccggggc
     2341 gttccacctt atcgggaaac gctgaattat gtggaaaaaa ttagagggcg ctataagaaa
     2401 gtaacgcatc cttatcaagc tgaggtcgta agccgcgctt cgatcttggt ttcagcgcga
     2461 taatcccgtt ttcttaggga gttcgttttg ttacctggat cgatttaatc acggatcaca
     2521 ctgacaagtg gttaaacctg ttctgttagt agcgttggtc attctgttac tcacgcatta
     2581 tcttaccgag cacgttaacg caagctggcg cacagcgagt cgtgagtcgg ttggattagc
     2641 tccaagtccg gaacaaactc cggaagcatt aattcaggtt tatggtgccc gtgcatatgg
     2701 ttggcgtggg tattttggag ttcatacctg gattgcggtc aaaccatcga atgcgaatgc
     2761 ttataccgtt tatgaagtaa tcggttggta tttaagacga gggggttcgg ttgttgccat
     2821 tcatgagcaa ataccggatc gccgctggtt tggaaatatg ccacagttgc ttattgaaaa
     2881 acgtggagag ggggttgatg cgctgattga gcgggttgag caagcagctt accaatatcc
     2941 ctatcattac gagtattgga tatggcctgg ccctaattcg aataccttca ctgcttggat
     3001 ttcacgtgct gtgcctgaac tagatttgga cttaccaccg acagcaattg gcaaagattt
     3061 tctagggaaa gaatgggtag caacagcgcc cagtggtcat ggatggcagt ggtcaatgtt
     3121 tgggctgctt ggagtgactg tgagtcggat tgaaggattt gaactcaata tcttggggtt
     3181 aacgtttggg attgaccccg atccatttgc gattaagtta ccactcatcg gtcgtatcga
     3241 tataccgatt ctagatttat tatcggcttc atcaacgatt caagaaaaat aattttctgc
     3301 aattaaatac gtgtactctt ttgcccgtaa ccgagcgttc cttgcaaaaa tcttagtgtg
     3361 taatagctaa tccagtttag cagtcgatcg agccaagcgc gttttttcca atatcttgca
     3421 gaaaccggat tggattcgtg cagtgcttgc tgtaagttgg cgcgtagttc tgaagaaaat
     3481 gcatgatcag taacgataat attggcttcc cgagctagca gcagactaaa ggggtcaatg
     3541 ttggatgaac cgacggttga ccagtattta tcgatgacag ctacttttgt gtgtaaataa
     3601 ctcttgtgat attcatagat tttgataccc gcctcaagca aatttccata tagtgcacgc
     3661 gaggcatagt gttggatacg gtattcaatt ttcccttgaa gcaataatat aaccctgaca
     3721 cctcgtcggg ctgcgtggat caacgccttt ctaaaatttc ttcctggtaa gaaataagca
     3781 ttagcgataa taatttcttc ttctgcggtg gcgattgctt ttaagtaagc atgctcgata
     3841 tcacgtcgat ggcggatatt atcgcgaatg accagtgcgg cacgctgatt accgcttgat
     3901 ttgagttgtg gttttaattc tggcagcttc ttaatccatc gttttttgaa gtgtacccac
     3961 gcaacgagca tccataatcg tttcaccaca ggataaatat ttgccaatag tgggccttct
     4021 attctgactg cgtgatcgaa ccggggtaac ggctgactcg ccctagggtc atgatcatcg
     4081 atgatattga tgccgccgat aaaggcaatt tgagcatcga ttaccacgac tttgcgatgc
     4141 atacgccgta atcgatgttt taagaactta aaggtaagca attccttacg gtagattagc
     4201 acttgaaccc cttccgtgat tagcttctct atgattttgt tggggaaatc aagtgatcca
     4261 aatccatcca tgagtaaatg aacggctacg cctcgtttgg ctgcccgggt tagtgcttct
     4321 gatatgatga ttcctgtggc atcgtactta aaaatataag tttggagatg gatttcgtat
     4381 ttggcttgct caatagctga tactaaagcg gggaagtact gagaaccatt gtttagtaat
     4441 atgattttat tgtgatcaat aaaatcaggg ttgttcatcg acgaacgata tttgcagtaa
     4501 gtgctgcgtg atcggatatt ctagcccacg ttgggctttg gtgaacgacg gcatttttta
     4561 cttggaaacc cctgacatag atacggtcta agcgcaataa tggaaatagg gaagggaagc
     4621 ttcgcgctgt tctgccatgg gtctgttcaa aaacttctac tagcttcatc gattcggtca
     4681 aaatacgttt tgcagagccg tgccaatcat taaaatcacc agcaataacg agtggggctg
     4741 ttggggggat gatttcagtc aaccgttttt caatggcacg cagttgcatt gcttggccac
     4801 gcttgaatag acctaaatgc acacaaatac agtggagtgg ttcttgccat cctggaatat
     4861 ccatttcgca gtggagtaag ccacgttgct caaaacgatg tgcggaaata tcgatatttt
     4921 cccaacggat aatgggaaat cgactgagaa tagcattgcc atggtgacct tcatcgtaaa
     4981 cagcgttctt accgtaagca aaatcgcgcc acacggaatc cgctagaaat tcatattgag
     5041 ggttcgaagg ccaattgcta aagcgcatgg cgtgcttgcg atgcgctccc accacttctt
     5101 gaagaaaaat gatatcagta cctagcgttc ttagtttttc ccgcatttca tgtaagacaa
     5161 tgcgctgatt gaacaaggaa aatcccttgt gaatattata cgttgcgatg tgtaaggtgt
     5221 tggtcataaa ttggcttaat caatcaattt tccagaacac aaagcgccgc agtatccaaa
     5281 agatgattca cttcctccta tttggagatc gctagcattc tttcaagcgc cagtttagct
     5341 aattgtgctt cctgatcggg tactttgatt tggtttacga ttttcccggc caccagattt
     5401 tctagtgacc aagccaagtg ttgtggatca attcgagcca tggttgaaca catgcaaacc
     5461 atgggcgaca tgaactgcac gatttttcct tgtgctttaa attcttcggc gattcgtccg
     5521 actagattta attctgtgcc tactaaccat cttgttccag gcgctgcttc gcgaatggtt
     5581 ttgatgatgt attctgttga accgacataa tccgatgctt tacagacttc aaagctacat
     5641 tccggatgtg agattacgat accatctgga tattggttgc ggaaacggat aatgtgctga
     5701 ggttgaaaca tttgatgaac tgagcagtgt cctttccata ataaaatttt tgcttttttg
     5761 atctgcgctt cagacaatcc tcccatcggt tcatcaaaat cccatatcaa catctcatcc
     5821 aagggtatgt tcattttata gccactccaa cgtcccaaat gttggtcggg gaagaataga
     5881 actttttcac gttgttcgaa ggaccactgc aagacttttg ttgcattact cgacgtacaa
     5941 acgatgcctc cgtgttcagc acagaaagcc tttagatcag cactggaatt aatataagtg
     6001 acaggggtga ttttttcatc aggattaaga atttctgaaa gctcacgcca ggcgcgctct
     6061 actttggaaa ggtttgccat atctgccatt gagcatcccg ctgagagatc aggtaagatc
     6121 gctatttgtt gcgggcttga gagaatatct gctacttctg ccatgaagtg tacgccacag
     6181 aataccaaat aatctgcatc ggtttgggca gccaagtagg aaagcttgag cgaatcgccc
     6241 gttaaatcag catgacagta aacatctgcg cgttggtaat gatgcgccag aataacggcc
     6301 tttttcccta gcttggcttt ggctgcaacg attctttgcg cacaaatttc atcttgtagt
     6361 aattcgtagt tttcaaaatc gattgtattc gcttgcatgg tcatgttaac ggttagccct
     6421 atatttgagt acgagttgct tttattatat ggtggttgga gatcaagttt aaagaggata
     6481 tcgatttaaa aacttgtcaa tgatcaagca ggggtaagcg ctaaaataat tccagctaaa
     6541 ggcggtaatg tgatgatcac ggaatcttga tattctaacc agggttctgc aacagaaaag
     6601 atttgtttgg gattaccgac atttccaccg ccgtagtagg ttgaatcgct attgaaaact
     6661 tcatgataaa taccccgtgc gggtacaccg atccgataat tctcccgtgg aaccggtgtg
     6721 aaattcagga cgatgaggct gaatgaccca tcttgcgcat aacgcaaata actgatgact
     6781 gattgatcgg cgtcattaca gtctgcccag caaaaacctg cttgttcaaa atcgagttga
     6841 tgaagtgcgg gtgtttcaag gtatagatga tttaagtcat gtatggctaa ttgtacgccg
     6901 cgatgcagct tgtaatcgag taggttccag tcgagttgct gattgacttg ccattcacgg
     6961 gcttgtgcaa attcattgcc catgaagttt agtttttttc ctgggcaagt catttggtag
     7021 gttaataata gccttaaatt tgcaaacttc tgccaagcat caccgggcat cttacctaat
     7081 aaagaccctt ttccatgaac gacttcatcg tgagaaaagg gtaatacaaa gttctcagaa
     7141 taagcgtaga gttgattgaa ggtaagctga ttatgatgat agcgccgata aatcggatcc
     7201 tggcgaatgt agctcaacgt atcattcatc cagcccatat tccacttcat cgagaaaccg
     7261 agtcctccca cataagttgg acgtgaaacc gcaggccatg cagttgattc ctctgcaaat
     7321 gtgagtgcgc ccggaaatgc atcatgaacc atgatattga gctcgcgcag gaaatcgatg
     7381 gcttcgagat tttcacgacc gccatattta ttcggtagcc attcgccgtg ttgacgggag
     7441 taatcgagat aaagcattga ggccacggca tctacccgta atccatcgag atgaaaatta
     7501 tttaaccagt aatgcgcact ggatagtaaa aacgatttca cttcattccg accataattg
     7561 aagataaaag taccccagtc gtgatgaaac ccgagccgag ggtcttcatg ctcgtagaga
     7621 gcagttccat caaatcgtgc cagtgagaaa ctatcttgcg gaaaatgcgc gggaacccaa
     7681 tccagaataa cgcctatgtt tgcctgatgg caggcattga taaaaaattt gaaatcctca
     7741 ggtgagccat agcggcttgt tactgcaaag taacctgtcg tttgatagcc ccacgattca
     7801 tcaaggggat gttcggaaat gggcaataac tcgacatggg tataacccat ttcaagtaaa
     7861 taaggaatta aatggtgcgc taactcacga tagttgaaga atttaccatc ggggtggcgt
     7921 ttccatgatc caacatgaat ttcatataga ttaagcggtg catgaagcca atcccaatgt
     7981 tttctttgat tcatccatag atcgtcactc catgtaaaac tggagtgagt cggtgtatat
     8041 gcagcattat tcggtcgaat ctcatgataa tttgcaaaag gatcggtttt tagtagtatc
     8101 tcaccgctat ggcgattacg aatttcatat ttgtaaatcc catgacctgg aaatcctggt
     8161 ataaataatt cccaaacacc actttgatga tgaacggcca tgggatggac acgcccatcc
     8221 cagccattgt gatctcctac gacactgact cgctcggcat tgggcgccca aaccgaaaag
     8281 cgtattcctg cgataccatt gattgtcaag tgatgcgccc ctagcattcg gtaggcttgg
     8341 tgtaatttac cttcattaaa taaatataaa tctaaatcag aaatttgcag tgggaacgag
     8401 taagggtcat aaaattcatg taaattttct ggtgccgcat catttttttt acgcattctg
     8461 atgcgatagc attttgggat tgaatcttta ccgcgccatt caaagagccc atcgacatga
     8521 gcacaggcca tttcagtaaa acctgcttca gtttgcagcc aaactttttc tgcatggggt
     8581 aagaaagctc ttacaacggt ttcattggaa accgggtgcg cgcctagata agcaaatgga
     8641 tcatgaagtc tagctgtaag cagctcatgc tgtagagggt aagaaacggt tgatagctgc
     8701 tttgatttca tggctagtaa tgctattagg gatcaagtaa gatggaacaa tagaagaata
     8761 aacaattcga tgaacagcca cagtttaaat cagcctgtgg cgatgggaaa gtgatacctg
     8821 actttagatt tcaaagacgc acttttttgt ttataaatca ttgggatagt aattcggagt
     8881 tagaaagtaa acttttttat cgggggaatg ttgtatggcg acacgaaata cccttgcact
     8941 aattttggca ggtggccgcg gcagtcgctt gcataacatg actgattggc gagccaaacc
     9001 ggctgtgccc tttggtggca agtttcgtat cattgatttt ccactttcta attgcgtgaa
     9061 ttccggtgtt cgtcggattg gggtcgtaac tcagtataaa gcgcaaagtt taattcgcca
     9121 tatccaaaga gggtggagtt ttcttgatgg tcgctttaag gaatttgttg aattattgcc
     9181 tgctcaacaa cgaacagaag aaacttggta tcaagggact gccgatgcgg tgtttcagaa
     9241 tttggatata ttgcgcaatc atgatcctag ctatgtattg attttaggtg gcgatcatat
     9301 ttataagatg gattacagca agctgctgga cgagcatatt caaaaaggcg cggatatgtc
     9361 tatcgcgtgt ttggaggttt cgtttgaaga agcgcgagcg tttggagtta tggcggttga
     9421 tgagcaatgg cgcataacga gctttgcaga gaaaccagag aaccctaccg cgattccagg
     9481 gcagccagac cgtgcgctcg ctagtatggg catttatgtt tttaattcac agtttttatt
     9541 tgagcaattg atacgggatc atgatgacaa tgcttcaacg catgattttg gaaaagatat
     9601 tatcccttat ttagtcaata gccaatatcg agtgtatgcg catcgcttta ccaatagttg
     9661 tgtcaacatg gcatcaggta ttccttattg gagggatgtg ggaacgattg atgcttattg
     9721 ggaagcaaat atcgatctga caaccgtgac tcccgatctt aatctttatg atgaggactg
     9781 gcctatctgg acataccaag agcaactgcc gcccgccaag tttgtttttg atgatgataa
     9841 tcggcgtggg caagcgctgg attccatggt gtcgggcggc tgtattatta gtggtgcgac
     9901 gattcgccgc tctttattgt tttccaatgt tcaggttaga agttttggaa taattgaaga
     9961 ttcggtgata ctgcctaacg tcgatattgg acgacacgca cgtttaaaaa atgttgttgt
    10021 agaaaaaaac tgcatcattc cagaagggct tagggtaggg tttgacgctg aagaggatgg
    10081 taagcggttt catgtaacta aaaaaggaat tacgttaatt acgcctgaaa tgcttgggca
    10141 gggtattcat gaattacgtt aagtgaggaa tttttaaatt ttttgttgga tcgtttggga
    10201 atgaattcgc ttaatttaat tttaatgtgg catatgcacc agcctgacta tcgggaccat
    10261 gaaactggtg aatttttgct tccgtgggtt tacttgcacg cgatcaaaga ttacaccgat
    10321 atggcctatc acttagagaa acatccaaga gtaaaggcag tgatcaattt tgtacctata
    10381 cttcttgaac agcttgaaga ttactctgat caatttttgc aagaaacgct gcgggatcca
    10441 ctactacgct tattgattac tccagatttg aacttgattt cactagagga tcgaatgttt
    10501 attttgaatc agtgttttca aagtaatcat gatacgatgc tcaaacccta ccgagcctat
    10561 cagcatttac gggatatcta tgaaacgttg ataggcaacg gggacgcaga gatgagttac
    10621 gtttcaggtc agtatcttgc tgatttgttg acttggtatc acctagcctg gacgggtgag
    10681 agtgttcgca aaaattgtga attagttgga aaactgatgc agcagggtaa aagttttacc
    10741 tatagtgatc gcaagcagtt atttgaattg atcggaacat taattcagga tttgatatcg
    10801 cgctatcgca aacttgcagt atcaggacag atcgagttat caactacccc acattatcac
    10861 ccactttcac ccttattgat cgatttcaat tcagcctacg atagccttcc tgatgctgtt
    10921 ttaccgtcaa gttctgatta tcctggagga cgagaccgtg ttgcttatca tcttgaattg
    10981 gctaaagaat ctcataaaca acgctttcat tcttacccat ttggtgtatg gcctgcagaa
    11041 ggtgggattt caaaggattt cgtagaaatt cttgctgcat cggagttgaa atggactgcg
    11101 agtggtgaag gggttttggc tcagagcttg cgtaaatcct atcctgatag gcagttacct
    11161 gagcggagtg agtatttata tcgcgcttat aaagtattaa caaataaaga agatgtgatt
    11221 tgtttttttc gtgatgacaa gttatcggat atgatcggat ttgagtatgc taaatggtat
    11281 ggtcgggatg ctgcagaaaa ttttgtacat tcactggaaa ggattcatca tgaggtaaag
    11341 gatgctgagg atcccgtggt tacgatcata ctagatggag aaaatgcatg ggaatcctat
    11401 ccttataatg gatactactt tctaaacgat ctttacgaaa tgatagggaa tcatttggca
    11461 attaaaactt ccacgtttcg agactactta gtgcatttaa aagaatcgga tactgaggct
    11521 gtttttctgc cgaatttggc cgcggggagc tgggtttatg gtaatttttc aacctggatc
    11581 ggaagccatg acaaaaattt ggcttgggat atcctatgta aggctaagaa aagttatgat
    11641 gcggtcttga aaataggtaa tttaaccgat gaggaacaag cagcagctaa acggcaactt
    11701 gcatcatgcg aaagttcaga ttggttttgg tggttcggcg attataattc ctcacaggct
    11761 gttgcaagtt ttgatcgcct atttcgcaag aatcttgcga atctatatag cctacttaaa
    11821 caaccaatac cgagtgaatt atcagaaccc attagctatg gcaacagtaa tgctcgcatg
    11881 atagaaacga tgcgacgttc aaattaaatc atttttttgt tgagtaatta ctcagaattt
    11941 ggttatgtca gaattgagta tgagtctatc cagacgcgtg taattattct acccttcgtg
    12001 agggaaattg agtcatgtca aaacccgtat cattgctttt tggtgttcat gcccaccagc
    12061 ctgttggaaa ctttccagag gtcttagaag acgcgcacat tcgctgctat aaaccttttt
    12121 tgcatgtagt acatcgatat cctgatttta aatttgcagt tcacttttct gggtggttac
    12181 tcgactattt attcgatcat catcctgagg atatgaaatt gctcaaagca atggtaaagc
    12241 gaggtcaagt tgagttgttt agcgcaggtg atacggagcc tgttctagca acaataccca
    12301 atcgcgatcg aatcggacag ctagaaacat tctctaaaaa attaaataaa aaattagggc
    12361 agcggcccca gggagcatgg ctaacagaga gagtttggga gtcaactgtt attcctgcat
    12421 tagttgattg tggaataagc tatgtcatcg tagatgatta ccattttctt tgtgccggaa
    12481 aaacgaaagc tgagctcaat ggctatttta cgacagaaga agataatcag agactggatc
    12541 tttttccgat ttctgaagcg ttacgctaca aacttccttt ttctcctgcg agtgatgcaa
    12601 ttgcatatat agagtcactc tcggaacatc aagcggcaag tcaacgagtt gcagcaatct
    12661 attttgatga tattgaaaaa tttggaattt ggcctgaaac ctaccaatgg gtttatgaaa
    12721 aagggtggtt agaacagttt atacaggggg tacttgcttc tcctcaaata atgacaagtc
    12781 attatcggga ttatcatagt tcagaaaaat ctcgaggaat catttatctt ccaacaactt
    12841 cttatatcga gatgaatgag tggactttgc ctgcagattt agcgaatcgg tatgctgatt
    12901 tagttcagca atctaaagta tcaggatcgt atgaacacaa taaacccttt ctaagggggg
    12961 gtatctggaa gaattttttc tcacggtatc ctgaatcgaa ttggatgcat aagcggatgc
    13021 tgagtttatc acagcgtttt gcctcgcttc cggttaaaca gcaaagcgca acaatgcgcg
    13081 agaagctcta tgcctcacaa gccaatgatg catattggca tggtttattt ggtggtttat
    13141 atcttccaca tcttaggcgt gctgttttca ataatatcgt tgagcttgaa gggatgttgg
    13201 atctaattgc accaagaccg actatctaca tagaagatac agatttggat ggagtagaag
    13261 agatctattt gaataatgga atattgcaag tgatttcaaa gcttgatggg gcagctagta
    13321 tttgtgaatt tgacgcttat aagcttaagc acaacttcgg tgataccctt cgtaggcagg
    13381 aagagcatta ttatcacaaa attaaatatg atatgcctaa tttacaacaa agtggatcgg
    13441 gtattgcatc ggcacatgat cggttgagtt ttaaacatca aattagctca gcggatttag
    13501 aagggggcag agcagcgcaa gcactttttg ttgaatctat cgatgatcac ttagtgatgt
    13561 accaatttga atcaaaacat gatcacggga taaaatttaa agcagacttc gatggttata
    13621 aaatcaataa aaagattgaa ttaattgaaa atcaattgct gataacgtat acttctaagt
    13681 cccgagtaga gggttacctc aagacagaat ttaacttggc tatgcctagt tgtgatggag
    13741 ttggtggcaa atacattaga gatgatttag ttttgggcgg attcgggcaa gaattagagt
    13801 taatggattt catcagtatc tttctagaag acagtacatt aaaaggtagg ttgttacttt
    13861 cggtatcaaa tgaaagtagg tttagtagta aaccttttta ttcagtatca cagtctgaag
    13921 gtgggtttga aaagattatg caggctatta aattggtgct agtttggcca tgcatacctg
    13981 agcgactgac gatttcgtta acggtagtgg agttaggcgg ggtggttgaa gtgtattctt
    14041 gacatatgga tttaaacccc atagaatccc caatcttttg tatctggcta tatgcgctta
    14101 tcggcctggc gtatagttag gtcaattatt atgcgttatg aaaattatgt ggctagtaag
    14161 ctacgagtta agcaagctag ctagttaaat aagttaaatg aaagatgtaa gtcatagatt
    14221 ataaaatgcc aacaacaagt caattaatta gaaagccacg gcaagataaa agagttaaga
    14281 gtaaggttcc cgctttggaa agttgcccgc aaaaaagggg tgtgtgtaca cgtgtatata
    14341 caacaacacc taaaaagcca aattctgctt tgaggaaagt tgctagagtg agattaacaa
    14401 atggcttcga ggtgtcgagc tatattggtg gagaaggtca taatctgcaa gagcattctg
    14461 tggtgttgat acggggtggg cgtgtaaagg atcttcctgg tgtaagatat catacggttc
    14521 gcggtagtct ggatacggcg ggagtgaaag atagaaagca aggtcgatct aagtatggtt
    14581 caaaaaaacc taaagtatct tagtcggaat ttggttgatt ttatttgaat ttttttcaag
    14641 gtaggtaaat gtcaaggcgt agagaaatac caaagcgaga aattttgcct gatccaaagt
    14701 atagcaatat tgaagtggct aaatttgtga atgttttgat gactcgcggc aaaaagtctg
    14761 tggctgaaag aattatttat ggtgcacttt cgcaagtaga gaaaaagacg ggtaaaaatc
    14821 ctgtagaagt ttttacaatt gcattgggca atataagacc gattgttgag gttaaaagtc
    14881 gtagggtagg tggtgcaaat tatcaggtgc cagttgaggt cagatcggtg cgccgtaatg
    14941 cgctggctat gaggtggctt agagatgctg cccgtaaaag aaatgagaaa tcaatggatt
    15001 tgcgtttggc tgtagaattg atagaggcat ccgagggacg tggcggcgca ataaagaaaa
    15061 gagaagaggt gcatcgcatg gcggagtcaa ataaagcatt ctcacattat cgattctaga
    15121 ggtttttaag attttttaga aagcgctaga gaattcagtt taattaaagt tagtaaggtt
    15181 taatgtgtct agaaaaacac ctatagagcg ttatcgtaat attggcatta tggctcatat
    15241 agatgccgga aaaacaacaa ctacggagcg ggttttgttt tacaccggag tgtctcacaa
    15301 attgggtgag gtgcatgatg gggctgcgac tatggattgg atggagcaag agcaggaaag
    15361 aggtataacg attacgtctg cagcaacgac atgcttttgg agcgggatgg ccaaaaatta
    15421 tccagagcat cgcattaaca ttatagacac tcctggtcac gtagatttta caattgaggt
    15481 ggagcgttcg ctaagggttt tggatggggc ttgtacagtt ttttgtgcgg tgggtggggt
    15541 tcagccgcaa acagagacgg tttggcgtca agcaaataaa tataaggtac caagattggc
    15601 gtttgtaaat aaaatggatc gatcgggtgc taattttaat cgagttcagg agcaaattaa
    15661 atcgagactt aaggcgttgc cagtgccgat tcaattgcca attggtgctg aggataaatt
    15721 tgaagggatt gtggatttaa taaaattaaa ggcgatctat tgggatgaaa cctctcaagg
    15781 aatgagatat gatgagcgtg atgttcctga gggtatgcta gattctgtaa aggctgcgag
    15841 agaatcaatg atagagtctg ccgcagaggc ttcggaagag ctaatgaata agtatctaga
    15901 aagtggagaa ttggctgcgg aagacataaa aaatggaata aggattagga cgcttgcaaa
    15961 cgaaattgtg cccatgcttt gcggctcggc atttaagaat aagggtgttc aagcaatgct
    16021 tgatgcggta atcgattttt taccatcacc gctagataag ccggcaatac agggattaaa
    16081 atcaaatgga gaaaaggatg agcgtcatgc aagtgatgaa gagccatttt ctagtctggc
    16141 ttttaagata gcgactgatc cttatgttgg gcaattgatt ttctttcgtg tttattcggg
    16201 agttgtaaat tctggtgata ctgtatataa ctcagttaag cagaaaaaag aaagagttgg
    16261 tcgattgctt caaatgcatg ctaatcaaag agatgagata aaagaagtgc gtgcgggtga
    16321 cattgctgct gcagtcggtt tgaaagaagt aatcacaggc gatacactat gtgatacgga
    16381 gcatataata acgctggaaa gaatggattt tcctgagcct gttattcatg ttgcggttga
    16441 gccaaaaaca aaaaacgatc aagagaaaat gggcatagcg cttaatagat tggcacaaga
    16501 agatccttcg tttagagtta gaaccgatga ggagtcaggt cagacaatta tatctggaat
    16561 gggtgagctc catttggaaa ttattgtaga tcgtatgaaa agggaattta gtgtggaggc
    16621 taatgttggt gctccgcaag tggcgtatag ggaagcaatt aaaaagaaag tagagattga
    16681 aggtaaattt atcaagcaaa gtggcgggcg aggacagtat ggccatgttt ggttaagaat
    16741 ggagccaaat gaagcaggga agggttttca atttattgat gagataaagg ggggggtggt
    16801 gccaagagag tacatccctg ctgttgagaa gggattgata gactctctta ctaatggcgt
    16861 attggctggt tatcccgtgg tggatgtaaa aatagcgttg tttgatgggt catatcatga
    16921 tgtagattca aatgagaatg cttttaagat ggcggcttca atcgcattta aagatggtat
    16981 gaaaaaagca accccgattc ttttggagcc gattatgtca gtcgaagtag aaactccccc
    17041 agactttatg ggtaacgtgg taggtgattt gtcttctaga cgtggcatga ttcaaggaat
    17101 ggatgataca gttggtttta aaattattaa agctgaagtt cctctggctg aaatgtttgg
    17161 atactcgaca gtattaagat ctgctacaca aggtagggca acttatacaa tggaatttaa
    17221 acattattct gaagcgccta aaagtgtggc tgaggcgatt attaataaga ataaataatt
    17281 ttgattttca tagattatta aattataggg tagaactgaa ttatggcgaa gagtaaattt
    17341 gagcgattga agccgcatgt gaatgtgggg acgata
//
LOCUS       DAOQKQ010000036        17206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_1502_length_17206_cov_6.559151, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000036 DAOQKQ010000000
VERSION     DAOQKQ010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 17206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17206
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1502_length_17206_cov_6.559151"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="PKZ49_03770"
     CDS             <1..863
                     /locus_tag="PKZ49_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HND35711.1"
                     /translation="IRSQRIRPGRDEKILTSWNALMIKGLTRAGVFFQCEAWIEAAQH
                     AIDFIHGNLWKDNRLLATYKDGKAHLNAYLDDYAFLLDSLLEMLQVQFRQTDFEFAIA
                     LAEVMLDQFEDKHLGGFFFTSHDHEKLIHRPKVGSDNAIPSGNGIAVIALQRLGYLLG
                     ESRYLQAAERALNLFYSEMLHHPSIYCSLLVALEEWLTPPKMIILRGDETRMKEWWRQ
                     IKSQTPYTFVIALPMELTGLSSSLNKPIPVNNDVNAWVCQGEKCLSEIVDLQELLQVC
                     KDKGKMAFPL"
     gene            886..1197
                     /locus_tag="PKZ49_03775"
     CDS             886..1197
                     /locus_tag="PKZ49_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HND35712.1"
                     /translation="MRNVWLSVLAASAVLLVGNVQADADLAKNSGCLNCHSVDTKLVG
                     PALKDIAAKYAGQDGAADMLAGKIKNGSNGVWGAIPMPPNANVSDENAKVLADFILTL
                     K"
     gene            complement(1249..1950)
                     /gene="flgA"
                     /locus_tag="PKZ49_03780"
     CDS             complement(1249..1950)
                     /gene="flgA"
                     /locus_tag="PKZ49_03780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HND35713.1"
                     /translation="MKKERILAFIGGILLLLSELVFGATTQSISSLLESAENFAYQGT
                     RDLPGSVRITLNKMDSNIALPKCSRLESFLPTGSRLWGKTSIGVRCNDQQAWTIYIQV
                     DIEVITKVIHSAKPILRDHTINEGDIVFKEVNLVRMSEGAFTDSEKVIGKVAVTNINV
                     GQPIYSQSVRAPYIIQRGQKVKLIVEGSGFSVSTEVEALSAAAEGQILQIRNQSGRIM
                     TGIARQDGIVEIKRK"
     gene            2200..2610
                     /gene="flgB"
                     /locus_tag="PKZ49_03785"
     CDS             2200..2610
                     /gene="flgB"
                     /locus_tag="PKZ49_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HND35714.1"
                     /translation="MINKLNKTLDFYHKALNVGAARHELLSSNIANADTPKFKARDVD
                     FMKTLQNAMTFNPSNMTTTSTMHMNGDRGFRSIASSELLYRIPQQPSIDGNTVDMDTE
                     RMQFINNSIKYDAGLTFINNQFRFVLSAIREGNA"
     gene            2613..3017
                     /gene="flgC"
                     /locus_tag="PKZ49_03790"
     CDS             2613..3017
                     /gene="flgC"
                     /locus_tag="PKZ49_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HND35715.1"
                     /translation="MSLFNVFNIAGSAMAAQSQRLNVVASNLANADSVASSNGQPYRA
                     RQVVFSTLSTATKEAKGVKVAGVIEDKSPMKVVFDPKHPMADAKGYVTMPNVNVVDEM
                     VNMMSASRAYQNSVNLMNTTKALLQKTLTIGQ"
     gene            3029..3703
                     /locus_tag="PKZ49_03795"
     CDS             3029..3703
                     /locus_tag="PKZ49_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HND35716.1"
                     /translation="MTQVQNSNPITSSFLDSVKTSNNTGKKEEIDPQDRFLKLLVTQM
                     KNQNPLKPLDNAEVTSQIAQISTVTGIEKLNTTLQSLVSDANSRRSLEAATMIGREVL
                     VPGSIMKLEEGEGLAGIELTEAADKVTMTITDSSGVKVREIDLGAKAAGINPIIWKGD
                     TESGAAAANGNYKFTVSAWRDGQEINVTSLAVGKVESVSTSDKGPTLDVGDLGHVNLS
                     DIKQIL"
     gene            3716..4993
                     /gene="flgE"
                     /locus_tag="PKZ49_03800"
     CDS             3716..4993
                     /gene="flgE"
                     /locus_tag="PKZ49_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HND35717.1"
                     /translation="MGFQHGLSGLRAASTNLDVIGNNVANANVIGFKQSQAQFSDIFA
                     NSLGGAGSNQVGIGTQVNTIAQSFSQGNISNSTNPLDMAIVGGGFFRLSDNGAISFSR
                     NGQFHLDKSGFLVNADNLNVTGYPADKNGKIIVSGPTNLQLSTAEMTPQATTTFETGV
                     NLDSRTLVPATLPAFDATDPDTYARSTSGTIFDSLGNSHVFTLFFQKSAPSTWDVYAT
                     VDGAVDASGVPVGVQLDGGSPLALTFDSNGVMTAPTGSVTASVDLSAVATNLGIADWG
                     ATTPLDFDFDITSSTQFGSNFAVNKLSQDGFTSGRLAGYGISDDGTIQGNYTNGENRT
                     LGQLVLASFANPNGLKPQGNNQWVETAASGLPLIGVPLTGTLGEIQSAAVEEANVDLT
                     TELVNLITAQRVYQANAKTIETQDAVLQTLVNL"
     gene            5046..5792
                     /gene="flgF"
                     /locus_tag="PKZ49_03805"
     CDS             5046..5792
                     /gene="flgF"
                     /locus_tag="PKZ49_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HND35718.1"
                     /translation="MDRLIYTAMTGAIHTMTQQATTAHNLANVSTAGYRAEVDSFRAV
                     PVYGDGLPTRAFVLDSTTGADFTPGPIHRTGRDLDVAVQGSGWIAVQLENGEEAYTRH
                     GNLQVSPNGLLTTTQGGAAVKGEGGAISLPPDSRVAIGKDGTVSMIPNDPRVTAVIVV
                     GRIKLVNPPPEQLKKGLDGLFRLKEGKEAPVDPNVVLVEGAIEGSNVNVVHEMVNMIS
                     LARQFDMQMKILDSAQQNSQRASEFMVYSG"
     gene            5827..6609
                     /gene="flgG"
                     /locus_tag="PKZ49_03810"
     CDS             5827..6609
                     /gene="flgG"
                     /locus_tag="PKZ49_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HND35719.1"
                     /translation="MIRSLWISKTGLDAQQTKMDVIANNLANVSTNGFKRMRPVFEDL
                     LYQTIRQPGAQSSQQTRLPSGLQIGTGVRPVATENIMLQGNFSQTGNTRDIAIKGEGF
                     FQIMLPDGTFAYTRDGAFQSDDQGQVVTSSGFVLQPPVSIPPNATSITVGRDGTVSAI
                     LPGATLSTQIGNIQLVSFINSAGLQRAGENLYFETVSSGPPNPNIPGTNGLGLLYQGF
                     VETSNVNVVEELVEMIQTQRAYEINSRSIQTSDQMLGRLTQL"
     gene            6665..7345
                     /locus_tag="PKZ49_03815"
     CDS             6665..7345
                     /locus_tag="PKZ49_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HND35720.1"
                     /translation="MLGLMIIIIGAAGCSLLTPAPKVHQPMTMQPHPVPSITPREANG
                     SIYHVAYSHPTQRYIPLFEDRRARGIGDTIIVNLNERTNASKRSASNIGRESQTSFSI
                     PSFLGLPLKKIFQGVDLETESENKFDGKGASSSNNNFTGTITVTVVDVLPNGNLLVSG
                     EKQVGINQGHEFIRLSGVINPINIMNNTVSSIQVADARIEYRGSGYLDEAQTMGWLSR
                     FFLTVLPF"
     gene            7400..8515
                     /locus_tag="PKZ49_03820"
     CDS             7400..8515
                     /locus_tag="PKZ49_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HND35721.1"
                     /translation="MLIFRRWILWFSLINCLILPSYSFAERIKDLASIQGVRANQLIG
                     YGLVVGLDNSGDMTTQTPFTVQSIVSMLGQLGINLPPGTNLMLRNVAAVMVTATLPAF
                     SRPGQLIDVTVSSMGNAKSIRGGTLLMTPLKGIDNQVYAVAQGNVLVGGAGGSAAGSS
                     VQVNHLASGRIPGGGIVERAVESALGQGEFVNLELHSTDFTTARRITEAINRIFPHGT
                     AYSVDGRVIQLKAPQDVNQRVAFISQIESLDVTPAQGIAKVIVNARTGSVVMNQTVTL
                     EASAVAHGNLSVIINSQPIISQPGAFAERGETVVTSQAQIEVRSDEGNLMMVQSGANL
                     AEVVQALNTIGATPQDLLAILQALKAAGSLRAELEII"
     gene            8541..9572
                     /gene="flgJ"
                     /locus_tag="PKZ49_03825"
     CDS             8541..9572
                     /gene="flgJ"
                     /locus_tag="PKZ49_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HND35722.1"
                     /translation="MKLLNTFDTSTQLAIDTKGIDNLRLQARQNEDEGLKQTAKQFES
                     LMVNMMLRSMRSATPKNGIFDSEQSQFYTQMLDQQLSQNLSSQGIGLAEMLVQQLKKD
                     SSGNLIDHPISEAESILSSIESSAGKNGLISLAPSEPKDLPNSLWTTPKQETNRPISM
                     FQSYQSTVMPLEVNKTNASKSMQSLQGKPNDFVNLVQPYARAIAKETGIPEQFMIAQA
                     ALETGWGKHQIRLSENQPSYNLFGIKAGKNWKGPVAEAVTTEYINGVAQKVVDKFRVY
                     RSYEESFRDYANLLLTNSRYSGVLKTQSAVAFAWGLQQAGYATDPYYAQKLIKILNSE
                     VFQINKIPS"
     gene            9667..11580
                     /gene="flgK"
                     /locus_tag="PKZ49_03830"
     CDS             9667..11580
                     /gene="flgK"
                     /locus_tag="PKZ49_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HND35723.1"
                     /translation="MGNSILNIGVSGLQAAQAALLTTSHNISNAHTPGYNRQQAIQST
                     NIPQPAGTGFLGQGVHISTVQRVYSEFLYSQGLQAQTQSSQLDSYYTQIKQVDNLLAD
                     TTSGLTPALHNFFKAVHDVASNPSSVPSRQSLLSNAEALVGRFQSMDQRLSEIREGIN
                     SQITSTVDEVNSLAKEIATLNINIQAAEGSTNGQPVNDMLDQRDTLVRQLSELVNVTV
                     VRQDNGHHNVFIGNGQALVVGSQSMALKALPSPDDPGKITIALDAGNNTILLPEKQLQ
                     GGSLGGMLDFRQNSLDKAQNTLGLIAIGLAQTFNDQHHLGQDLNGALGEDFFEVPEPK
                     VISLAGNSSTSTITASISSVGDLNTSDFQFNYDGSQYTLTRLSDNVSVSSATAPSIGS
                     PLTLSGISVTAATIQPNESFVIQPARNGAKDIATAINDTSKIAAAAPIRSQAASTNTG
                     SATISAGIVNPLPIDPDLSQSVTIKFHTPSDGMFDVTGTGASLPAMNQTYTEGADISF
                     NGWTVQISGVPEAGDEFTISPNTGGIADNRNALLLAGLQTKNTLQDGTASFQSTYAQL
                     VSQVGNRTRELEVTSKAQANLLAQTDQSIQALSGVNLEEEAANLLRYQQAFQASGKVI
                     EVSHSLFDTLLRI"
     gene            11615..12841
                     /gene="flgL"
                     /locus_tag="PKZ49_03835"
     CDS             11615..12841
                     /gene="flgL"
                     /locus_tag="PKZ49_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HND35724.1"
                     /translation="MRVSTNTMYDQGVQSMLKQQEALFKLQQQISVGKRIVTPSDDPV
                     AAARAHEVSQAASINSQYAENRDKAKSSLGLVESNLQGVSTIIQNVRSIAVAAGNPIL
                     SESDRRVMAIELRGRLEELIGLANSADSEGNFLFSGYQAKVQPFVKNAGDVQYAGDQG
                     QRLTQVTSSRQIAVSDTGANIFERVMDGNGVFTTSANATNIGTGIIGVGSVTDPTNLT
                     GDDYEINFHVTQDPLTLKDIITYDVLNTTTSSTISTGNTFVDGNMIAFDGLQFDIKGK
                     PADGDQFKVEPSSQQSVFKTIDNLINVLETTTNDQSGKTNLTNSLNTAMQNLDQGLDN
                     VLSIRASVGVKLQEIDALQSIGEDNALQYDQTLSRLQDLDYAKALSDFTRQQGLLEAA
                     QQSFIRISGLSLFNRL"
     gene            complement(12857..13909)
                     /locus_tag="PKZ49_03840"
     CDS             complement(12857..13909)
                     /locus_tag="PKZ49_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HND35725.1"
                     /translation="MDSKHSIDRHFLFWVLLIAVSGFLTYLLSPILTPFLLAAVIAYI
                     CNPFVSWLNTHNISRTIGTILVMVLSLAIFTALILVMMPLFEREILRLIERTPEYLET
                     IRETGIPWLKSTLGIDLQLDIAVLKQAFTEHWKSAGGIAAKMLPSLTSGGMAILDFIM
                     NLLLVPVVLFYLLRDWNSLLAEFNEWIPSIWKKQIYPMVREADRILAEFLRGQIAVIL
                     LMCIFYVTGLWLTGLEFSLPIGLVAGILVFVPYLGAIVGLSLATFAALVQFQDWGDII
                     WVLVVFGSGQLLESMLVTPWLVGDRIGLHPVIVIFALLAFGQLFGFIGILLALPISAV
                     LLVWLRHLHQQYLNKT"
     gene            13971..15032
                     /gene="purM"
                     /locus_tag="PKZ49_03845"
     CDS             13971..15032
                     /gene="purM"
                     /locus_tag="PKZ49_03845"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HND35726.1"
                     /translation="MTSSDSKYSTADQLSYRDAGVNIDAGNLLVERIKPFAKRTWRPE
                     IIGGIGGFGALFEISNKYRQPVLVSGTDGVGTKLKLAFHLNRHDRIGIDLVAMSVNDI
                     LVQGAEPLFFLDYFACGKLNVDIAAKVIEGIAVGCEQAKCALIGGETAEMPGMYPEGE
                     YDLAGFAVGVVEKDQIIDGTSIAENDVILGMASSGAHSNGFSLIRKILEYHPVDSDSK
                     LVDDVKLLDAIMEPTRIYVKAILELKNHIPIKGLAHITGGGLLENIPRVLPANLRAVL
                     HKGSWAVPPLFTWLQAHGNVADEEMYRVFNCGIGMIIVLAPEYVDEALAVLKAYNETV
                     WQIGTVQPNPTNDQRVLIV"
     gene            15044..15679
                     /gene="purN"
                     /locus_tag="PKZ49_03850"
     CDS             15044..15679
                     /gene="purN"
                     /locus_tag="PKZ49_03850"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HND35727.1"
                     /translation="MQSLVILISGRGSNLQALITANLPVKSIVVISNNPSASGLAVAQ
                     SHQIATHVIDHRCYLDRDSFDAELARQIDRYQPDLIALAGFMRILGDKFINRYHGKLI
                     NIHPSLLPSFPGLNTHARALEQGVKIHGCTVHFVTNQLDHGPIIIQAAVPVFPDDMPD
                     MLANRVLKQEHLVYPTAVRWFLESRIKLKDGQVKISSALNVDQTLYSPAID"
     assembly_gap    15751..15850
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            15860..17119
                     /locus_tag="PKZ49_03855"
     CDS             15860..17119
                     /locus_tag="PKZ49_03855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HND35728.1"
                     /translation="MRLTTIQLEMAIQAIRKILPLNFPADILMRGFFRENPMLGHNDR
                     AIIAEIVFGILRHKYFLDTLAEKATPRALLLAYLAKFQGINSRELISFATESEIKWLA
                     HVKGAQIAMQPLAIQAELPQWLIEKLQTYLPDEEILKLGVALQQPAPLDLRVNTLLAK
                     REEILTQLEQAEVKANAMPYSPIGIRLAEKTAINQHPLFLEGKVEIQDESSQLSVYLL
                     APRRGEMIVDFCAGSGGKTLLIGALMQSHGRIYAFDVSDKRLNNLKPRLKRSGLSNIH
                     PIRIDNENDLRVKRLAGKIDRVLIDVPCSGLGTLRRNPDLKWRQSPESIEELKIKQRA
                     ILNVASKLLKPGGRIVYATCSLLPEENEEIVESFLANHTQFIALNCGELLAQQKIPLN
                     TGNYLQLSPANHHTDGFFAAALELKKN"
BASE COUNT         5232 a         3229 c         3783 g         4862 t
ORIGIN      
        1 aaatacgcag tcaacgtatt cgtccaggtc gtgatgaaaa gatattgact agctggaacg
       61 cgctgatgat taaaggatta acgcgtgctg gtgttttctt tcaatgtgag gcatggatag
      121 aggccgccca gcatgctatt gattttatcc atggtaatct ttggaaagat aatcgattgc
      181 ttgcaactta taaggatggt aaagcgcact taaatgccta tctcgatgat tatgcatttt
      241 tgttggatag tttgttggaa atgctccaag ttcaatttcg tcagacagat tttgagtttg
      301 caattgcact tgctgaggta atgcttgatc aatttgaaga taagcattta ggaggatttt
      361 tttttacaag tcatgatcat gagaagctaa ttcatcgccc taaagttggg tcagataatg
      421 cgataccttc aggaaatgga atcgctgtaa ttgcattgca gcgactgggt tatttacttg
      481 gggaaagccg ttatttacag gctgctgagc gtgccctgaa tttgttttat tctgaaatgc
      541 tccatcatcc cagtatttac tgcagcctgt tggtggccct agaggagtgg ttaactccgc
      601 caaaaatgat tattcttcgc ggggatgaaa ccagaatgaa agaatggtgg cggcaaatta
      661 aatcacaaac accctatact tttgttatcg ctttgccgat ggaactaact ggtttgtcga
      721 gtagtctgaa taaaccaata cctgtaaata acgatgtcaa tgcatgggtt tgtcaaggtg
      781 aaaaatgctt gtcagagata gtagatttac aggaattgct gcaagtctgt aaagataagg
      841 gtaaaatggc attcccttta taattttcaa aataaggaat atacgatgag aaatgtttgg
      901 ttaagtgttt tagctgcatc agcagtatta ttggtaggaa atgtacaagc agatgctgat
      961 ttagcaaaaa atagcggttg cttaaattgc catagtgttg atactaaatt ggtaggccct
     1021 gctcttaaag acatcgcagc aaaatatgcg ggtcaagatg gtgctgctga tatgctagct
     1081 ggaaaaatca aaaatggtag taatggtgta tggggagcca ttccaatgcc acccaatgcg
     1141 aatgttagtg acgaaaatgc taaagtttta gcagacttta ttttaacact taaataattc
     1201 tagtttactg agtgtgataa agccgatgta atgattacat cggctttttt attttcgttt
     1261 tatctcaacg ataccatctt ggcgcgctat cccagtcatg attcgacccg attgattgcg
     1321 tatttgtaaa atttgtccct cagctgcggc agaaagcgct tcaacctcag tactgacact
     1381 gaaaccagac ccctctacaa tcaacttgac tttctgccct ctctgaatga tatagggagc
     1441 tcgtaccgat tgactatata taggttgtcc cacattaata ttggtaactg caactttacc
     1501 gattactttt tctgaatcgg tgaatgctcc ttcagacatt cgtaccagat ttacttcttt
     1561 gaatacgata tcaccttcat ttatggtgtg atcacggaga atgggttttg cagaatgaat
     1621 tactttagta ataacttcta tgtcgacctg aatataaatt gtccacgctt gctgatcatt
     1681 acagcgaaca ccgatagatg tttttcccca tagccgactt ccagtaggta ggaaggattc
     1741 taatcttgaa cattttggta gtgcgatatt tgaatccatt ttgttaagag tgatcctaac
     1801 cgacccagga aggtcgcgag tgccttgata agcaaagttt tcagcggact ctaagaggga
     1861 tgatatggat tgagtagttg ctccaaaaac caactcggaa agtaataata aaattcctcc
     1921 aataaacgcc agaatccgct cttttttcat accctcatca ccattcatgg tgtaaatgtt
     1981 ctagaacaat tgctacacaa gagaattttt ttgccggtca tggcaatttt tgccgcctgc
     2041 aaaagatagt agcgttaaat tccggtgcct aaatcgtgaa ctgaagacta atttctactt
     2101 atttagaata tttcgttgtg tttttaaagc aagaaagact ggcatggtta atgcgtatat
     2161 aagaaagcag aaagataaaa ctttttagga aagaaagcga tgatcaataa gctaaataag
     2221 acacttgatt tctatcataa agccttaaat gtcggtgcag ccaggcacga gttgctatca
     2281 agcaatattg cgaatgcgga tacacctaaa tttaaggcaa gagatgttga ttttatgaaa
     2341 acgctacaaa atgccatgac atttaatcct agcaatatga cgacaacttc aacgatgcac
     2401 atgaatggtg atcgtggttt tagatcaatt gcttcttcag aattgttata tcgaattcct
     2461 cagcagccaa gtatagacgg taatacggtt gatatggata ccgaacgcat gcagtttatc
     2521 aacaactcga tcaaatacga tgctgggcta acgttcatca acaatcagtt tagatttgta
     2581 ttgtcagcta tcagagaagg aaacgcataa aaatgtcact atttaatgtc tttaatattg
     2641 ctggttcagc gatggctgct caatcacaac gactcaatgt ggtcgcaagt aatttggcga
     2701 atgcagatag cgtggcaagt tcaaatggcc aaccctatcg cgcgcgacaa gttgtattta
     2761 gcacattgag cactgctacc aaagaagcaa aaggtgtaaa agttgcgggt gttatcgaag
     2821 ataaatcacc catgaaggtt gtttttgacc cgaagcaccc aatggcagat gcaaaaggct
     2881 atgtcaccat gcctaatgtc aatgtggtag acgaaatggt caatatgatg tcagcctcgc
     2941 gcgcttatca aaatagcgtg aatttgatga ataccacaaa agctttgttg cagaaaacgt
     3001 taactatcgg tcaataagag gaacagctat gactcaagtg caaaattcaa acccaattac
     3061 atcgtctttc ttggactcag ttaaaacatc aaacaatact ggaaaaaaag aagagattga
     3121 tccacaggat cgatttttaa aactactggt tacacagatg aaaaatcaaa atccattgaa
     3181 accattggac aatgcggaag taaccagcca gatcgcacag attagtacag taacaggtat
     3241 cgagaagctt aatactacgc tacaaagttt agtgagtgat gcaaactcaa gacggtcttt
     3301 agaagcagca accatgattg ggcgcgaagt gttagtaccc ggcagcatta tgaagctaga
     3361 agagggggaa ggcctggctg gtatagagct aactgaagca gcagacaaag taacgatgac
     3421 catcacggat agctctggtg tcaaagttcg tgagattgat ttaggagcaa aagctgctgg
     3481 tatcaatcct attatttgga agggtgatac tgaaagtggt gctgctgcag ccaatggtaa
     3541 ttataaattt acagttagtg catggcgcga tggacaagag atcaatgtga cgtcattagc
     3601 agttggcaag gttgagagcg tatctaccag tgataaagga cctacattgg atgtgggaga
     3661 tctggggcac gttaatttat ctgatattaa acaaatactc taagaggagc gcattatggg
     3721 ttttcaacat ggcttaagtg gcttaagagc agcatcaacg aatttagatg tgataggtaa
     3781 taatgtggcg aatgcaaatg tgattggatt taagcaatcc caagcgcagt tcagtgacat
     3841 ctttgcaaac tcactcggtg gcgcaggaag taatcaggtc gggattggaa ctcaagtcaa
     3901 cactattgct caatcctttt cacaagggaa tatcagtaat tcaactaatc cattggatat
     3961 ggcgatcgtg ggtggtgggt tctttcgctt aagtgataat ggtgcgatta gcttcagtcg
     4021 caatggccag tttcacctcg ataagagtgg attcctggtt aatgcagata acttaaatgt
     4081 gaccggttat cctgctgata agaatggaaa gatcattgtg agcggaccaa cgaatttaca
     4141 actttcaact gccgaaatga ccccgcaggc tacaacaaca tttgaaacag gagtcaatct
     4201 tgattcgcgc acgctggtac cagctacttt accggctttt gatgctaccg atcctgacac
     4261 ttacgctagg tccacttcag gaactatctt tgatagttta ggcaactcac acgtgtttac
     4321 cttatttttt caaaaatcag cgccaagtac ttgggatgtc tatgcgactg tcgatggtgc
     4381 agtcgatgct tcgggtgtgc ccgttggtgt tcaattggat ggtggttctc cgttagcact
     4441 aactttcgac agtaatgggg ttatgactgc gccaaccggt tcagtaactg cgtcagtaga
     4501 tttgagcgct gttgcaacca atctaggaat tgctgactgg ggtgctacga caccactcga
     4561 ttttgatttc gacattacca gctctacaca atttggtagt aattttgcgg taaacaaact
     4621 gagtcaagat ggatttactt caggcagact cgctggctat ggtatttctg atgatggcac
     4681 tatccaaggt aattacacga atggagaaaa tagaacactg ggacaacttg ttctggctag
     4741 ttttgcaaat cccaacggtt tgaagccaca aggaaataat caatgggttg aaacggcagc
     4801 ttctgggtta ccactgatcg gtgtgccact aactggaacg ctcggagaga ttcaatcggc
     4861 agcagttgaa gaagcaaatg ttgatttgac gacagagctt gtcaatttga taacagcgca
     4921 acgggtttac caagcaaatg ctaagaccat cgagacacaa gatgctgtat tacaaacact
     4981 cgttaatctt taatcagaat ttatttttta tttgatttga gtgattgaac ttacaggatt
     5041 tagctatgga ccgattgatt tacactgcca tgactggcgc aattcatacg atgactcaac
     5101 aagcaacaac tgcccataat ttagccaatg tttcgactgc cggctaccgt gctgaagttg
     5161 attcattccg ggcagtacct gtttatggag atggattgcc aactagagcg tttgtattag
     5221 attcaaccac tggcgctgat tttacaccgg ggccgattca tcgtacgggt cgtgatttgg
     5281 acgtggctgt acaagggtcg ggatggatcg cggttcagct agaaaacggg gaagaagcct
     5341 atacgcgtca cggtaatcta caagttagtc ctaatggatt attgactaca acccaaggtg
     5401 gcgcagcagt taaaggtgag gggggcgcca tttcacttcc accggatagt cgtgtggcaa
     5461 ttggtaaaga tggaacggtt tcaatgattc ccaatgatcc aagggtgacc gcagtgattg
     5521 tagtcggtcg aattaaactc gtgaatccac cgcctgagca gctcaagaag ggccttgatg
     5581 gcttattccg gttaaaagag ggaaaagaag cgccagtcga cccaaatgtt gtattagttg
     5641 aaggagcgat tgagggaagc aatgtcaatg ttgtgcacga gatggtcaat atgatttcgc
     5701 ttgcgaggca attcgatatg caaatgaaaa tcttagatag tgcacagcaa aattcccaac
     5761 gtgccagtga atttatggtt tatagcggtt aattagtagc attctgacag aaaaggtaaa
     5821 aaaattatga ttcgttcatt atggatatca aaaacagggt tagacgcaca acaaaccaag
     5881 atggatgtca ttgctaacaa cttggcaaat gttagcacga acggttttaa acggatgcgg
     5941 ccagtttttg aagacttgct ctatcaaact atccgtcagc ctggggccca gtcttctcag
     6001 caaacacgtc tgccatcagg acttcaaatc ggaacagggg tacggcctgt tgcaacagaa
     6061 aatattatgc ttcaaggtaa tttttcacaa acaggaaata cgcgggatat tgccattaaa
     6121 ggggaaggct tttttcaaat catgttaccc gatggtacat ttgcttatac gcgtgatggt
     6181 gcattccagt ccgatgatca aggtcaggta gttacatcga gtggctttgt tttgcagcct
     6241 ccggtttcaa ttccacctaa tgctaccagc attaccgttg ggcgtgatgg aactgtatca
     6301 gccatattgc caggcgctac attgtctacc cagatcggca atattcagct tgtaagcttc
     6361 attaattcag ctggtttgca aagagcagga gagaatttat attttgaaac cgtctccagt
     6421 gggccaccta atcccaatat ccctggtaca aatgggctag gtttactcta tcaaggtttc
     6481 gttgagactt ctaatgttaa tgtggtcgag gagttggttg aaatgataca aactcaaagg
     6541 gcctatgaaa tcaattcgag atctatacaa acttccgatc aaatgcttgg taggctaact
     6601 cagctataac atcatcaata tgcgaaatct tattaacctc atacttagaa gcaataagat
     6661 cagaatgttg ggattgatga taataatcat cggggcagcg ggttgttcat tgctgacacc
     6721 cgctccaaag gttcatcaac cgatgacgat gcaacctcat ccggttccca gtatcacacc
     6781 tagagaagca aatgggtcaa tttatcatgt tgcttacagc catcctaccc agcgctatat
     6841 tcccttattt gaggaccgcc gtgctcgtgg aattggtgac acaatcattg tcaatctcaa
     6901 tgaaagaacg aatgccagta aaagatctgc gagtaatatc gggcgagaaa gccaaactag
     6961 cttttctatc ccatcgtttt tgggattgcc attaaagaaa atctttcaag gcgtagatct
     7021 tgaaacagag tcagaaaata agtttgatgg aaagggtgca agttccagca ataacaattt
     7081 caccggaacc attactgtta cggtggttga tgttttacct aatggaaact tactggtaag
     7141 cggtgaaaag caagttggta ttaatcaagg acatgaattt attcgtcttt ctggtgtaat
     7201 taatccaatc aatatcatga ataacacggt ttcttcaatc caggttgcgg acgcacgtat
     7261 tgaataccgg ggaagtggtt atctggatga agcgcaaacg atgggttggt tatctcgatt
     7321 ctttcttacc gttttgccat tctaatcatg caatagctga ttcatttatc gattcaaaac
     7381 ctaactaaga gacatgagta tgttgatttt ccgtagatgg atattatggt ttagtttaat
     7441 aaactgcctg attctaccga gctatagttt tgctgaacgt atcaaagatc ttgcaagtat
     7501 ccaaggagtg agggccaatc aattgattgg ctatggcttg gtcgtagggc tggataatag
     7561 tggcgatatg acaacccaaa ctccttttac ggtacaaagt attgtcagta tgctcgggca
     7621 attaggtatt aatttaccgc ctggaactaa tctgatgttg cgcaatgttg ctgcggttat
     7681 ggttaccgca actttaccag cattctcaag gccagggcag ttgattgatg ttacggtttc
     7741 atcaatggga aatgctaaaa gtattcgggg aggaacatta ttgatgaccc ctttaaaagg
     7801 aattgataat caagtttatg cagtcgcaca agggaatgta ctggtgggcg gtgctggagg
     7861 ttctgcggca ggcagtagtg ttcaagtaaa tcatttagca tcgggtcgta tccccggtgg
     7921 aggtattgtt gagcgtgcgg ttgaatcagc tttgggtcag ggagaattcg tgaatcttga
     7981 gttacattcc acagatttca cgactgcacg acggattacc gaagctatta accgtatatt
     8041 tcctcacggg acggcttact ctgtggatgg tcgtgttatt caacttaagg cgccgcagga
     8101 tgttaatcaa cgtgttgcat ttatttctca aattgaaagc cttgatgtga cccctgcgca
     8161 ggggatagca aaggtgattg ttaatgctcg aaccggttca gttgtcatga accaaaccgt
     8221 aactttagaa gccagtgcag tagcgcatgg caacctttct gtcatcatta acagccagcc
     8281 gattattagt caaccaggcg cttttgctga acggggagaa acggttgtta cttcacaagc
     8341 acaaattgaa gttcgctcgg acgaagggaa cctcatgatg gtacagagtg gtgcaaattt
     8401 ggccgaggtt gtgcaagcgc taaataccat tggcgcgaca ccacaagatt tgttggctat
     8461 tttgcaagcg ctaaaggctg caggctcact gcgtgcagag ttagaaataa tttaatcgta
     8521 atgttcaaca aggctgttgc atgaagttac taaacacatt tgatacctca actcagttgg
     8581 caattgatac caagggtatc gataaccttc ggctacaagc aagacagaac gaagacgaag
     8641 gattgaaaca aactgcaaag caatttgagt cattaatggt caatatgatg ctaagaagta
     8701 tgcgaagcgc aacgccgaag aacggtatct ttgatagtga acagtcacag ttttatacac
     8761 aaatgctcga tcagcaactt tcacaaaatc tttcttccca agggatagga ttagcggaaa
     8821 tgctggtcca gcagcttaaa aaagattctt ctgggaattt gattgatcat ccaatctcag
     8881 aagctgagtc aatcctatca tccatcgaat caagtgctgg aaaaaatggg ttgatttcat
     8941 tggcgccaag tgaacctaag gatttgccaa attcgctgtg gacaaccccg aaacaggaga
     9001 cgaataggcc gatatcaatg ttccaatcat atcagtcgac agtgatgccg ttggaagtaa
     9061 ataaaacaaa tgcttccaaa tcaatgcaat cattgcaagg taagccaaat gattttgtta
     9121 atttggttca gccttatgcg agagccattg ctaaagaaac aggtattcca gagcagttta
     9181 tgattgcaca ggcagcgctt gaaactgggt ggggaaaaca tcaaattcgt ttgtcagaaa
     9241 atcaacctag ctacaatctt tttggaatta aagcaggaaa gaactggaaa gggcctgtgg
     9301 ctgaggccgt tacgactgaa tatattaatg gagtagcaca gaaagtagtc gataaatttc
     9361 gtgtttatcg ttcatatgaa gaatccttcc gtgattatgc caatcttctc ttaacgaatt
     9421 cacgttacag tggggtactt aagactcaaa gtgcagttgc ttttgcttgg gggctacagc
     9481 aagcggggta tgcaactgat ccatactatg cgcaaaagtt aattaaaatc ttgaatagtg
     9541 aagtattcca aattaataaa attccaagtt aaagagcttc taattgctta actgatgaat
     9601 atttttgccg ttaacaatac tagttgaaat tgatttaatt gtaatatgcg ataaaggaac
     9661 aagataatgg ggaatagtat attaaatatt ggagtttcag gacttcaagc agctcaggca
     9721 gctttattga cgactagcca caatattagc aatgcgcata caccaggtta taatcgacaa
     9781 caggctatac agagtactaa tattcctcaa ccggctggaa caggatttct tggtcaaggt
     9841 gttcatattt caacggtgca gcgagtttac agtgaatttc tttatagcca aggattgcaa
     9901 gcgcaaacac agagtagtca gctagatagt tactatactc agattaagca agtggataat
     9961 ctgcttgcgg atacgacttc aggccttacg cctgcattgc ataatttctt taaagcagtg
    10021 cacgatgtgg cttccaatcc ttcatcagtc ccttcaaggc agtccctgtt gagtaatgca
    10081 gaagctctgg ttgggagatt ccagagtatg gatcaacgtc tatcggaaat acgcgaaggt
    10141 atcaatagtc aaataactag tactgttgat gaggttaatt cattagctaa agaaatcgct
    10201 acattaaata tcaatattca ggctgcagaa ggttcaacta atgggcaacc ggttaatgat
    10261 atgcttgatc agcgtgatac attagtcagg caactgagtg agctagttaa tgtgacggtt
    10321 gtacgtcaag ataatgggca tcataatgtt tttattggta atggtcaggc gcttgtagtc
    10381 ggatcgcagt caatggccct taaagcactt ccttcccctg atgatcctgg caaaattact
    10441 attgcgctcg atgcaggtaa taatactatt ttattacctg aaaaacagct acaaggtgga
    10501 agtttgggtg gaatgctaga ttttcgccag aactcattag ataaagcaca aaatacacta
    10561 ggattaattg caattggact cgcccaaact tttaatgatc aacaccattt aggtcaagat
    10621 ttaaatggtg cgctgggtga agattttttt gaagttcctg aacccaaagt aatatcgtta
    10681 gcggggaata gttcaacttc aacaataaca gcgagtatta gtagtgtagg tgatcttaat
    10741 acgagtgatt ttcagttcaa ctacgatgga tcacagtata cgttaacaag gttgtctgat
    10801 aatgtatcag ttagttcagc aacagcccca tccataggga gcccactcac attaagtggt
    10861 atcagtgtta ccgctgcaac tattcaaccc aatgaaagct ttgtaattca acctgctcgt
    10921 aatggtgcaa aagatatagc aaccgctatt aatgatacat ctaaaattgc agcagcagcc
    10981 ccaattcgtt cgcaagccgc aagtacgaat accggatcag ctacgattag tgctggaatt
    11041 gtaaatccat tacccattga tcctgattta tcacaatcag taacgattaa gtttcataca
    11101 ccttctgatg gtatgtttga tgttactgga acaggagcga gtttgcctgc catgaatcaa
    11161 acctacactg aaggtgccga tattagtttt aatggctgga ctgtgcaaat tagtggagtc
    11221 cctgaagctg gtgatgagtt tacgatttct ccaaatacag gaggtattgc tgataaccgg
    11281 aatgcactat tgttagcggg attgcaaacc aagaatacac ttcaagatgg gacggcctca
    11341 tttcaatcaa cttatgcaca attggtcagt caggttggta atagaacacg agagctcgaa
    11401 gttactagta aagcacaggc taatttactt gctcaaacag atcaatcgat acaggctttg
    11461 tcaggtgtta accttgaaga agaagcagct aatttgctac gttatcagca agctttccaa
    11521 gcgtctggga aagtcattga ggttagtcat tcgttgtttg atactttact cagaatttaa
    11581 aagaaccgat tcaaattaga ggagttaacc gagcatgcgc gtaagtacaa ataccatgta
    11641 tgatcaagga gtgcaatcaa tgcttaagca acaagaagct ttatttaaac tccagcaaca
    11701 aatctccgtg ggcaaaagaa ttgttacgcc atccgatgat ccagtagcgg ctgcacgtgc
    11761 tcatgaagta tcccaagctg cttcgattaa ttctcagtat gctgagaatc gtgataaagc
    11821 aaaatccagt ttgggcttag ttgaaagtaa tcttcaaggt gtttcaacaa ttatacaaaa
    11881 tgttcggtct attgctgtgg ctgcgggcaa cccaattttg tctgaaagtg atcgaagggt
    11941 aatggctata gagctaagag gtcgattgga ggaattgatc ggattagcta atagtgcaga
    12001 tagcgaaggt aattttttat tttctggtta tcaggcgaaa gttcaacctt ttgtaaagaa
    12061 tgcaggtgat gttcagtatg ctggtgatca aggacaacgt cttacccaag tcacgagttc
    12121 tcgtcagatt gcagtgagtg acacaggtgc aaatatcttt gaacgggtca tggatggtaa
    12181 tggggttttt actacttcag caaatgctac taatattggg actgggatta ttggtgttgg
    12241 ttcggtaact gatccaacta atttaacggg cgatgactat gaaataaatt ttcatgtgac
    12301 ccaagatccg ctcactctaa aagatatcat tacctatgat gtgttgaata cgacaacaag
    12361 ttctacaatt tccaccggta atacttttgt ggatggaaat atgattgctt ttgatggatt
    12421 acagttcgat attaagggta aaccagctga tggcgatcaa tttaaagtag aacccagttc
    12481 tcaacaaagt gtttttaaaa cgatcgataa tttgattaac gttttagaaa cgacaacgaa
    12541 tgaccaaagt ggtaaaacaa atttaacaaa ttcattgaat actgctatgc agaaccttga
    12601 tcagggtctg gataatgttt tgagtatacg agcatcagtt ggagtgaaac tgcaagagat
    12661 cgatgcttta cagagtattg gagaagataa tgccttgcaa tatgatcaaa cactctctcg
    12721 attacaagat ctggattatg caaaagcgtt atctgatttt acaagacaac aaggtcttct
    12781 agaagctgcg caacaatctt ttattcgtat ttcagggcta tcacttttta acaggctata
    12841 aaagaaacat ttaatgctat gttttattga ggtattgctg atgcagatgt ctaagccata
    12901 ccaataaaac agcgctgatg ggaagcgcca ataatatacc aataaaaccg aaaagctgtc
    12961 caaaagcaag cagagcaaag ataactataa ctgggtgtaa gccaatacgg tctcccacta
    13021 gccagggtgt aaccagcata ctttctaata attgaccaga accgaataca actaaaaccc
    13081 agataatatc tccccaatct tggaattgga ccagtgcagc aaacgtagcc aatgacaaac
    13141 caacgatcgc acctaaatag ggaacgaata ctaaaattcc agcaactagg ccaataggta
    13201 aagagaactc taatccagtc aaccatagtc cagttacata gaagatacac attaataaaa
    13261 taacagctat ttgcccccta aggaattcag caagaattcg atctgcttcg cgtaccattg
    13321 ggtaaatctg ttttttccat atggatggga tccattcgtt gaactctgca agtaaactgt
    13381 tccaatctcg taataaataa aagagtacga cgggtaccag tagcagattc ataatgaaat
    13441 ctaatatagc catacctcca cttgtcagag aaggtagcat tttggctgca atgcctccag
    13501 cgcttttcca atgttcagtg aacgcttgct ttagtacagc aatatctaat tgaagatcaa
    13561 tcccaagcgt agattttagc catgggattc ctgtttcacg aattgtttca agatattcgg
    13621 gtgttcgttc gattagccgc aaaatctctc gctcgaataa gggcatcatt actaggatta
    13681 atgccgtaaa aatggcaagt gataaaacca tgaccagaat tgttccaatc gttcgagaga
    13741 tattatgagt gttcaaccaa gaaacgaatg gattacagat ataagcaata actgctgcta
    13801 atagaaacgg tgttaatatc ggacttagca gataagttaa aaaaccagaa acggcgatta
    13861 gtagcaccca aaaaagaaag tgacgatcaa tagaatgttt ggaatccatt tgcggtaaaa
    13921 ttacgaatgc aaatagtgcc actctaactg taaccaattg agaactcaac ttgacttcat
    13981 ctgattctaa gtattctact gctgatcaac tctcgtatcg tgacgcgggc gtcaatattg
    14041 atgcgggtaa tttattagta gaaagaataa aaccctttgc aaaacgtacc tggcggccag
    14101 aaattattgg agggattgga gggtttggcg cactatttga aatttcaaat aaatatcgac
    14161 aacctgtttt agtttcagga actgatggtg tgggtaccaa gctaaaatta gctttccacc
    14221 ttaatcgcca tgatcgtatc ggtatcgact tagttgcaat gagtgtcaat gatattttag
    14281 tccagggtgc tgaacccctc ttttttctag actacttcgc ttgtggtaag ttgaatgtcg
    14341 atatagcggc gaaagtcata gaaggtattg cagtaggctg tgagcaagct aaatgtgcac
    14401 ttattggtgg ggaaacagca gaaatgcccg gaatgtatcc tgaaggggaa tatgatttag
    14461 caggttttgc agtaggcgtt gtagagaagg accaaataat tgatggaacc tcaatcgctg
    14521 aaaatgatgt aattctgggt atggcctcaa gtggggcgca ttctaatggt ttctcactta
    14581 ttcgtaaaat tcttgaatac catccagttg attcagattc taagcttgta gatgatgtta
    14641 agctcctaga tgcaattatg gaacccacac gtatctatgt taaagccata ctagaattga
    14701 aaaaccatat accaattaaa ggcctggctc atatcacggg tggaggtctt cttgaaaata
    14761 ttccacgtgt tcttccagca aatcttagag cagtgctgca taaaggttct tgggccgtac
    14821 caccattatt tacatggctg caagcacatg gaaatgttgc tgatgaagaa atgtatcgag
    14881 tttttaattg tggaatagga atgataatcg ttctagcacc ggaatatgtt gatgaagctt
    14941 tggcagtgct taaagcttat aatgaaaccg tctggcaaat cggtacagtt caaccaaatc
    15001 ctacaaatga tcaacgtgta ttgattgttt gatttgggaa atgatgcaat cgttggtcat
    15061 tcttatttct ggtcgaggta gtaacttgca agccttgatc acagcaaact tacctgtcaa
    15121 gtcaatcgtc gttattagta acaatccttc ggcatcagga ctagcagttg ctcagtcgca
    15181 tcagattgca acgcatgtta tcgaccatcg atgttatctg gatcgtgatt catttgatgc
    15241 cgaacttgct agacaaatcg atagatacca acctgattta attgcactgg ctggttttat
    15301 gcgtatactt ggtgataaat tcattaatcg ctatcatggt aagttaatca atattcaccc
    15361 atctttacta ccgtcttttc caggactaaa cacccatgcg cgagctttgg agcaaggtgt
    15421 aaaaattcat ggttgtaccg ttcactttgt aacgaatcaa ttagatcatg gcccgattat
    15481 tattcaagcc gcagttcctg tttttcctga tgatatgccc gacatgttag ctaatcgggt
    15541 cctaaagcaa gaacatttgg tttaccctac cgcagtacgc tggtttttag aaagccgtat
    15601 caaactaaaa gatggtcagg tcaagatatc atcagccttg aatgtggacc aaacgctcta
    15661 ttcacccgct attgattaga agttcttccc ccgggttttc aaagagataa tgagtagtag
    15721 ctatctacac aactagcgtg taattattaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15841 nnnnnnnnnn ctattcatca tgcgcttaac aactattcaa ctggaaatgg ctattcaagc
    15901 tatcagaaaa attcttcccc tcaattttcc tgccgacatt cttatgcgtg gatttttccg
    15961 tgagaatcct atgctggggc ataatgatcg agccattatt gcagaaattg tgtttggtat
    16021 tttacgccat aagtattttt tagacacact agctgaaaaa gctacgccgc gcgcgctttt
    16081 gttagcttat ttagctaaat ttcaaggtat aaattcacgt gagttgataa gttttgcaac
    16141 cgagagtgaa ataaaatggc ttgcacatgt aaaaggtgct cagattgcaa tgcagccgct
    16201 tgcgatacaa gcggagttac cgcaatggtt gatcgagaag ttacaaactt acctgccaga
    16261 tgaagagatt cttaaacttg gagttgcatt gcaacaacct gctcctttag atctgcgggt
    16321 taatacgttg cttgcaaagc gtgaggaaat actaacccag ctagaacaag cggaagtaaa
    16381 agcaaacgca atgccttact caccgattgg aattcggtta gcagaaaaaa ccgcgatcaa
    16441 tcaacatcca ttatttcttg agggaaaagt agaaattcag gatgagagta gtcaattatc
    16501 tgtgtactta cttgcgccgc ggcggggaga aatgattgtt gatttttgtg cgggttctgg
    16561 cggaaaaacg ttgctgatag gggcactcat gcagtcacat gggcgtattt atgcattcga
    16621 tgtttcggat aaacgtctta ataatctgaa acctagactt aaacgttctg gtttgtctaa
    16681 tattcatcct atacgtatcg ataatgagaa tgatttaaga gttaaacggt tagcaggaaa
    16741 aattgataga gtacttatcg atgtgccgtg tagtggttta ggcacattac gccggaatcc
    16801 agatttgaaa tggcgacaat ctccagaaag tatcgaagaa ctaaaaatta agcaaagggc
    16861 tattctaaac gtagcaagta agctattaaa accgggtgga aggatcgttt atgctacgtg
    16921 tagtttactc ccagaagaga atgaagaaat cgttgaaagt tttttagcaa atcatactca
    16981 gtttatagcg ctaaattgcg gtgagttgct ggcccaacag aaaataccgc ttaatactgg
    17041 aaattattta caactgtcac ccgctaatca tcatacggac ggcttctttg cagcagcact
    17101 tgaacttaag aaaaattagc ttttttaagt aaaagaacag aagatatttt agaatatgcc
    17161 tctaaactat tttcagttta atctgggtgt gactcaaaca ggcatt
//
LOCUS       DAOQKQ010000037        17146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_1512_length_17146_cov_5.692470, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000037 DAOQKQ010000000
VERSION     DAOQKQ010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 17146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17146
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1512_length_17146_cov_5.692470"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2715
                     /locus_tag="PKZ49_03860"
     CDS             <1..2715
                     /locus_tag="PKZ49_03860"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HND35729.1"
                     /translation="LSVFQDAISIRAGDRWVTKLEEALRHCSAFVVLIGGEGIQRWVG
                     AEVQVALLRHLSPHDDAERLPIFPVLLDDVEPNTLPSFLQLFQATHWTPAEQLPKDLI
                     QAIRTHRIRINSQHGREDRCPYLGLSAFTRNDAKFFFGRRQEILEALSCLGDQQQTNP
                     EALNKSGGAVYHRWLQIEGNSGSGKSSLVQAGMLPMIEQGALWARTGFDQWTIIGPMM
                     PGKKPLQKLAETLEQGLIRDPEKRDSLSRFNRLKEDKSGLALASTIKDFKQPQVNGAF
                     LLIVDQFEELFTFAEEDERKQFDKLLSNALQDTNCPLFLISTVRADFLDRFEDLQHLQ
                     ALYNSHCKRYFLPTISVKGLQEIIEQPAQLAGLDVQEITAVILKDAENEIGALPLVEN
                     ALTLLYQHQQQPNKLSGDYYRQQGGIAGMLSEEADKLLAHIDQAVPQGKLATLELLLR
                     LTRINDEGRHARQRITLEEAISIAGNGNDESGRRVIAMLSGQRDINLLNAGHQNVLRL
                     ITLNEESGQHYVDLIHETLIRARSKEVGSGKRTGYWPALFEYIENNRDRDFYRQQLVI
                     ESKRWQQSKGLGRLWHLTYWGLHKYKDLRIPRNSIEGRFRVWSKRMQFALIVLLSGMA
                     LYVAESIYWIQDHDLPPDMIWTLQHFRFGDPVLPELTDKPIPAGSFAMGEQDAVFSAN
                     LGEYEKNFGVPGKAVSITQPFHLSNTEVTYQQYDYYVWQQHRIGNTQVKFPNTANGGR
                     DNQPVVNVSWIEATAYANWLGEQIHRQCRLPTEAEWEYAARGGTQTAYPWGDKPGNNN
                     ANCGGCGSQWDGKASSPVGSFAANAFGLKDMSGNVWEWTCSNWRDQFDGSEQRCNNDS
                     NDTQFRVLRGGSWLNSPDYLRVSLRNYALPDFRLNDFGFRVLCLSPIE"
     gene            complement(2931..3251)
                     /locus_tag="PKZ49_03865"
     CDS             complement(2931..3251)
                     /locus_tag="PKZ49_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HND35730.1"
                     /translation="MTTTKRKQRILDEMHETARGLHHAGLISKRRMREFDALCHLDVE
                     EMSPQKIKSLREQARVSQAVFAAVLNTSLSTVQKWEIGDKKPSGPSLKLLNLIERKGL
                     EAVL"
     gene            complement(3232..3618)
                     /locus_tag="PKZ49_03870"
     CDS             complement(3232..3618)
                     /locus_tag="PKZ49_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HND35731.1"
                     /translation="MRRVFLTRTFSRWMRKVGLPNDAICHAVFEMSQGLIDADLGGNV
                     VKKRIGLSGQGKRGGVRVIVATKMADRWFFLYGFGKNERANIDKNELKVLQEVAKELL
                     ELNNQQLAIALSAGEMIEVCNDNNKT"
     gene            4070..5557
                     /locus_tag="PKZ49_03875"
     CDS             4070..5557
                     /locus_tag="PKZ49_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HND35732.1"
                     /translation="MTLDSNIQRAPGNAGWRLDNSYARLPDYFYAQLSPVPVRAPQAV
                     LLNHALAESLGLGLQALSAEEGAALFAGNILPEGAQPIAQAYAGHQFGHFNMLGDGRA
                     ILLGEHLTPDGRRVDIQLKGSGQTPFSRRGDGRAALAPMLREYIISEAMHALGIPSTR
                     SLAVVTTGEQVQRETMLPGAILTRVAASHIRVGTFEYAAGHGGKEAVQILADYTIQRH
                     YPELQNSDNPYFALLNRVIERQAALVAQWSMVGFIHGVMNTDNTSISGETIDYGPCAF
                     MDAYNPDTVFSSIDQNGRYRYRHQPMMMQWNLARFAETLLPLFDPSQQQAVVMAEESI
                     RAFPGIYQTHWIDGMRKKLGLFTAEENDSELIAGLLDWMTQHQTDYTNTFRALTEPAL
                     PQDAMFRESSFMEWHSRWQQRLARQPQPISESMQLMQANNPVIIPRNHRVEEALTAAT
                     EQGDYTVLQRLLAVLAQPYEDKPEYCDYRIPPAPSERVYQTFCGT"
     gene            5594..5977
                     /locus_tag="PKZ49_03880"
     CDS             5594..5977
                     /locus_tag="PKZ49_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HND35733.1"
                     /translation="MSITLNHTIVPSFDKVESAKFYSRVFGFEYVGAFSHFIVVRVND
                     TLCLDFDNETQFESHHYAFKVSEREFDDIFARLLAEKIPYGSGPGSANDMAINHNYGG
                     RGVYFCDPNRHLLEMLTADYVMPPQ"
     gene            complement(6073..7284)
                     /locus_tag="PKZ49_03885"
     CDS             complement(6073..7284)
                     /locus_tag="PKZ49_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HND35734.1"
                     /translation="MPLVDMRDMLDHAYQNNYAIGGFGLVSLDFLHAILSAAEYCRSP
                     VIFNLSEPLFKHFGTQLILSSIEHAAHHAKVPVAIHFDHGQSYESIVQAIKLGCNSVM
                     LDASKDTFPANATQTTRVTEMAHGCGVAVEGALGFVGGSEGENAISHLGEIIFTSAEE
                     AEAYVTRTHVDFLAVSIGTSHGRQPSRAKLDFKRLKRIDETLKIPLVLHGGSGLVEEQ
                     YHKLILSGIAKINCYTELSDLAAHQIQANLNAQSYNGYIEAMTGVHDRLFERIKQYMQ
                     FWGSAGRAAEVLIQCRHWNSVEQIILFNPNQPQNIDLLSAQGSALLNKIPSVRNVFLG
                     KAITEPTRYQLCWHIQLSHEDALPHYLAHPSYTAFINQCCYTLSSERINITFTPSHFA
                     KNHELQHIDRL"
     gene            complement(7329..7685)
                     /locus_tag="PKZ49_03890"
     CDS             complement(7329..7685)
                     /locus_tag="PKZ49_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35735.1"
                     /translation="MIEADTLALPLRKFPKKINAKCYNRGRLAILRIGQPLRIALEHH
                     RGLEVILNNTIWLCVDSFADDQPVLAWREFRTQDRNQLHRPIACELWLYHSCAGLIMG
                     SALEDLQQTLEKLTLH"
     gene            complement(7729..8637)
                     /gene="cbbX"
                     /locus_tag="PKZ49_03895"
     CDS             complement(7729..8637)
                     /gene="cbbX"
                     /locus_tag="PKZ49_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbX protein"
                     /protein_id="HND35736.1"
                     /translation="MTTETVSVDLEAEFQDSNIKEVLEKLDRELIGLKPVKTRIHEIA
                     ALLLVDRVRKQLGLSAGAPSLHMCFTGNPGTGKTTVALRMSEILHRLNYVREGHLVSV
                     TRDDLVGQYIGHTAPKTKEVIKKAMGGVLFIDEAYYLYKPENERDYGAESIEILLQTM
                     ENNRDDLVVILAGYKDRMDKFFYSNPGMRSRIAHHIDFPDYSANELLAIAKLMLAAQN
                     YRFSPSGENAFSEYLQLRMKLEHFANARSVRNALDRARLRQANRLFTSAKKTLTSIDL
                     MTIEEEDIRSSRVFLEGKLDTGSSKI"
     gene            complement(8770..9204)
                     /locus_tag="PKZ49_03900"
     CDS             complement(8770..9204)
                     /locus_tag="PKZ49_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose bisphosphate carboxylase small subunit"
                     /protein_id="HND35737.1"
                     /translation="MMTNTGNRLTQGQFSFLPPLTDKQISSQIKYALKKGWAIGIEYT
                     DDPHPRNTYWEMFGNPMFDLKDPAGILMEINNCRKTFPNHYIRVTAFDSSRGVESPTM
                     SYIVNRPENEPGFSLMRQESSSRNINYTIHSYATDKPEGERY"
     gene            complement(9222..10697)
                     /locus_tag="PKZ49_03905"
     CDS             complement(9222..10697)
                     /locus_tag="PKZ49_03905"
                     /EC_number="4.1.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-bisphosphate carboxylase large subunit"
                     /protein_id="HND35738.1"
                     /translation="MASETIKEGKERYKSGVIPYKKMGYWEPDYIPQDTDVIALFRIT
                     PQEGVEPEEAAAAVAGESSTATWTVVWTDRLTACELYRAKAYRVDPVPNTGPGTKTDQ
                     QYFAYIAYDLDLFEEGSIANLTASIIGNVFGFKAVKALRLEDMRIPVAYLKTFQGPAT
                     GIVVERERLDKFGRPLLGATTKPKLGLSGRNYGRVVYEGLKGGLDFMKDDENINSQPF
                     MHWRDRFLFCMEAVNKAAAATGEVKGHYLNVTAATMEDMYERAEFAKSLGSVIVMIDL
                     VVGYTAIQSMAKWARKNDMILHLHRAGNSTYSRQKNHGMNFRVICKWMRMAGVDHIHA
                     GTVVGKLEGDPLMIRGFYDTLRETNIERNLEHGIFFDQGWASLRKVMPVASGGIHAGQ
                     MHQLIDYLGEDVVLQFGGGTIGHPQGIQAGATANRVALEAMIMARNEGRDYVAEGPKI
                     LAEAAKWCSPLKLALDTWKDITFNYESTDTADFVPSTTMSQ"
     gene            10868..11824
                     /locus_tag="PKZ49_03910"
     CDS             10868..11824
                     /locus_tag="PKZ49_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HND35739.1"
                     /translation="MRHSTIRQLEVFEAIARLKSFTRAAEELFLTQPTVSMQIKKLTD
                     DIGLPLFEQVGKKIYLTDAGKELYQTCLGIFEHFERFEMIASNMRGLKAGKLRLAVVT
                     TAKYFTPRLLGMFCKKYPGIEVKLKVTNRERVLERLLHNQDDLYILGQVPEEIDAVAA
                     PFLDNPLVVLASTNHPLAKARNIPVQSLSEEAFIMREPGSGTRMATERFFTDRGLKIN
                     IRMELGSNEAIKQAIVGELGIAVLSRHTLALDAPMGQLVMLHVEGFPLKRSWYYAYPS
                     GKQLSIVAETFLEYLRQAPDFLVDLSCYHASVGCCPLLNKSN"
     gene            12032..12511
                     /gene="rnhA"
                     /locus_tag="PKZ49_03915"
     CDS             12032..12511
                     /gene="rnhA"
                     /locus_tag="PKZ49_03915"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HND35740.1"
                     /translation="MQNTVQKIEIYTDGACKGNPGVGGWGALVMYDGHTQELFGGEVS
                     TTNNQMELMAAIRALQALESLPLAESARVHLFTDSQYVQKGISEWIENWKKRGWRTAD
                     RKPVKNESLWKTLDQLSQRYSIEWCWVRGHTGHNGNERADALANRGVETVMVGNHSD"
     gene            12526..13224
                     /gene="dnaQ"
                     /locus_tag="PKZ49_03920"
     CDS             12526..13224
                     /gene="dnaQ"
                     /locus_tag="PKZ49_03920"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HND35741.1"
                     /translation="MRQIFLDTETTGLDVKSGHRIVEIAAVEVCNRQLTGRHFHYYLN
                     PERESEASALQIHGLTTEFLQDKPKFPDICEAFIQFIENAELLIHNAPFDVSFLNNEL
                     VLCNQNTLSSYCTEIIDTLILAKELHPGKRNNLDALCERYQVDHSHRVLHGALLDSEL
                     LAEVYLRMTRGQDSLLMDFDTPVLDISTDEALKGLQLVVLSADPSELERHAKRLEAIN
                     KVSKGNCLWQSLND"
     gene            13279..13773
                     /locus_tag="PKZ49_03925"
     CDS             13279..13773
                     /locus_tag="PKZ49_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Appr-1-p processing protein"
                     /protein_id="HND35742.1"
                     /translation="MIHEVQGDILLSKAQVIIHGVAPNDHFDSGLALSLREQWPAMVK
                     DFRHFCQTTHPKSGAAWLWSGTGGVHILNLLTQEEALGHQTKPGKASTENVNHALRAG
                     RHIIEKEGFRSVALPRLATGVGGLDWNSVMPLIQNHLGDLAIPVYIYAQFIKGKQALE
                     PSNP"
     gene            complement(13789..14124)
                     /locus_tag="PKZ49_03930"
     CDS             complement(13789..14124)
                     /locus_tag="PKZ49_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HND35743.1"
                     /translation="MIIEIKTILIFLLTAVAEIVGCYLPYLWLKEGKSIWLLVPAALS
                     LALFAWLLSLHPTAAGRVYAAYGGVYIAVAIFWLWAIDGIKPTIWDIIGACVALIGMA
                     IIMFAPRNT"
     gene            complement(14114..14875)
                     /locus_tag="PKZ49_03935"
     CDS             complement(14114..14875)
                     /locus_tag="PKZ49_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HND35744.1"
                     /translation="MAELERYLTKQTLKIFEVHEKKERTYQAYSATALFDFIFGSDWR
                     AAEEIVFICEDQFQPSIPVTKFLQYNAYFAFASADNHPFTLKNLLQNNEKVHLGPLYL
                     VWDNLSSKELQYEGAVDMPYQIIGIELTTFSARFPAIFPPNDTSAEVQRGFLHFRKHC
                     FACHTINGQGSGKAAELNYPVSVTEYIQPTYLKLWIDNPTAIRLNSKMLSLAADIPDR
                     DQVIDEIIAYLTAMRRVKPQSKTMHIQHNNQSDDN"
     gene            complement(15042..16082)
                     /gene="tsaD"
                     /locus_tag="PKZ49_03940"
     CDS             complement(15042..16082)
                     /gene="tsaD"
                     /locus_tag="PKZ49_03940"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HND35745.1"
                     /translation="MIVLGIETSCDETGIALFHTEKGLLSHTLYSQIEMHNEYGGVVP
                     ELASRDHINRVLPLIKQNLAIAQIDLKAINAIAYTQGPGLAGALLVGASIANALAFAL
                     RIPILEIHHLEGHLLSPLLTNPTITFPFVALLVSGGHTQLIRVDKVAHYALLGETLDD
                     AAGEAFDKTAKLLGLGYPGGKSLSLLAERGESGRFTFPRPMRNSGDFNFSFSGLKTSV
                     QTLIKHIEITDQIKADIALAFQEAVVDILVEKSLAALKHCSLSNLVVAGGVGANSQLR
                     QRLTLEASQLGANVHFPELALCTDNGAMIAFAAAMRISSGDKNYLETHSNYRYTVKAR
                     WNLETTAYNHCI"
     gene            complement(16139..16894)
                     /gene="zapD"
                     /locus_tag="PKZ49_03945"
     CDS             complement(16139..16894)
                     /gene="zapD"
                     /locus_tag="PKZ49_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008057722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HND35746.1"
                     /translation="MICYEHPLNERVRTLLRLENLFSKVDFFSKCNTPTEHHSALVAL
                     FEILDVTSRADLKSDLLQELEKQRTMLESLRKNPDVSEDALNRVLTDIKVTFRDLLNI
                     PGKIGGHLRDNEWLMSIKQRMSIPGGACEFDLPSYHYWLSLSPEHRHQDFKVWIEPLI
                     PIREGFKIVLMLLRNSGRSYHYTAKQGQFQQTGSDHMAHMIRLRIDENLPCVPEISAN
                     KYALSIRFIPFSPEQKVKVYEDDIEFELTLCNL"
BASE COUNT         4566 a         3719 c         4041 g         4820 t
ORIGIN      
        1 ttaagtgtat ttcaagacgc aatttccatt cgtgcaggcg atcgctgggt aacgaaactt
       61 gaagaagctt tgcggcattg ttcagctttt gtggtattga tcggcggtga aggcattcag
      121 cgctgggtgg gtgcagaagt ccaagtggct ttattgcggc atctgtcacc gcatgatgat
      181 gccgagcggt tgccgatttt tccggttctg ctcgatgacg tagaaccgaa cacactgcca
      241 tcgtttctcc aattgttcca ggccacacac tggacacctg ccgaacagtt accgaaagac
      301 ctgattcagg ctatacgaac acatcgtatc cgcatcaatt ctcagcatgg ccgcgaagat
      361 cgctgccctt acctcggact cagcgctttt acgcgcaatg atgccaagtt tttctttggg
      421 cggcgccaag aaattttaga agccctgtcc tgcctcggcg atcagcagca gaccaatccg
      481 gaagcgctca ataaaagtgg tggtgctgtt tatcaccgct ggttgcagat tgaaggcaac
      541 agcggttcgg gcaagtcctc actggttcaa gcaggcatgt tgccgatgat tgaacaaggc
      601 gcactgtggg cacgcacggg cttcgatcaa tggacgataa tcggcccgat gatgcccggc
      661 aagaagccgt tgcaaaaact ggctgagacg ctggaacaag gattgattcg tgaccctgag
      721 aagcgtgatt cactgagtcg attcaatcgg ctcaaggaag ataaaagcgg gctagcgctg
      781 gcatcgacca tcaaggattt caaacagccc caggtcaatg gcgctttttt gcttatcgtc
      841 gatcaatttg aagagctatt cacgtttgcc gaagaagacg aacgcaaaca gtttgacaaa
      901 ctgttgtcca acgcattgca agacacaaat tgccccttgt ttctgatcag cacggttcgt
      961 gccgattttc tcgaccgatt cgaggatttg cagcacctgc aagccttata taacagtcat
     1021 tgcaaacgtt attttctacc gaccatctct gttaaaggct tacaggagat catcgaacag
     1081 cctgcccagt tagccgggct cgatgttcag gaaataacgg cagtaatatt gaaggatgct
     1141 gaaaatgaaa tcggcgcatt gcctttggtg gaaaatgcct tgacgctatt gtatcaacat
     1201 cagcagcaac ccaataaact cagcggcgat tattaccgcc agcagggcgg tattgccggt
     1261 atgctcagcg aagaagcaga caaattgctg gcacacattg accaggccgt accgcaaggt
     1321 aaattggcaa cgctggaact gttactgcgt ctgacgcgaa ttaacgacga agggcggcat
     1381 gcacgtcagc gtattacgct agaggaagcc atctctatcg cgggcaacgg caatgatgaa
     1441 tccggcagac gcgtgattgc aatgctttct ggacaacgcg acatcaacct gctaaatgcc
     1501 ggacatcaga acgtgctgcg gctgattacg ctgaatgagg agtctgggca gcactatgtc
     1561 gacctgatcc atgaaacatt gatccgtgcg cgcagcaagg aagtcggtag tggcaagcgc
     1621 accggttact ggcctgcgtt gtttgaatat attgaaaata accgtgaccg tgacttctat
     1681 cgtcagcaac tggtcattga gagcaaacgt tggcagcaga gcaagggtct cggtaggctg
     1741 tggcatctaa cctactgggg actccataaa tacaaagatt tgcggatacc ccgtaacagt
     1801 atcgaaggtc gtttccgggt ctggagtaaa cgcatgcagt ttgcactgat tgttctactg
     1861 tcaggcatgg ccttgtacgt cgcagaatcg atttactgga tccaggatca tgacttgcca
     1921 ccggatatga tctggacgct gcagcatttc cggtttggcg atcccgtatt gcctgagttg
     1981 accgacaaac cgattccggc cggatcattc gctatgggtg agcaggatgc tgtgtttagc
     2041 gcgaatctgg gtgaatatga aaagaacttt ggtgttcctg gaaaagctgt gtcgatcacc
     2101 cagccttttc atctcagtaa caccgaagtg acttaccaac agtatgatta ttatgtttgg
     2161 cagcaacacc gcatcggtaa cacacaagtt aagtttccaa ataccgctaa tggcgggcgt
     2221 gataatcagc ctgttgtgaa tgttagctgg attgaggcta ccgcgtatgc gaactggctt
     2281 ggtgagcaaa tacacaggca atgccgtttg ccgaccgaag cggaatggga atatgcggcg
     2341 cgtggcggta cgcaaacagc ttatccctgg ggagataaac caggcaataa caatgccaat
     2401 tgcggtggtt gtggcagtca atgggatggt aaagcatcat cgcctgtagg cagttttgca
     2461 gccaatgcct tcggactgaa agacatgtcg gggaatgtct gggaatggac ctgctcaaac
     2521 tggcgtgatc aatttgatgg cagtgagcaa cgctgtaata acgatagtaa cgatacgcag
     2581 tttcgcgtac tgcgcggtgg ttcctggctc aatagtccag actacctgcg cgtgtcgctc
     2641 cgcaactacg ctcttccgga cttccgtctc aacgatttcg gttttcgggt gttgtgtttg
     2701 tcccccatcg aatagccgat cgctggtgca ctgagcactg aaacgctggg tggctgttca
     2761 cggatagcgc cgcgcagcgg cgtgcgattt ttttaaccgc agatcacccg atttaatgcg
     2821 aaatgaccgc agaacatgtc gccgcaatcc ccatgtgttt cccgcagtgt tcagcaattt
     2881 ttatcaattc ggctaaaaat tgctaacgat tgttcacttc aactggaaaa tcagagcaca
     2941 gcttccaatc ctttgcgctc gatgagattt aatagcttca aggaagggcc gctgggtttc
     3001 ttgtcaccga tctcccattt ctgcactgtg gagaggctgg tgttgagtac ggctgcaaaa
     3061 acagcttggc tgacacgcgc ctgctcgcgc agagatttaa tcttttgcgg ggacatctct
     3121 tctacgtcga gatgacataa ggcatcgaac tcgcgcatgc gacgcttact aatcaagcca
     3181 gcatggtgca gtccacgggc tgtttcgtgc atctcatcta gaattcgctg cttacgtttt
     3241 gttgttgtca ttgcatacct ctatcatctc accagcagaa agagcgatcg ccaattgttg
     3301 gttattcagt tccagtagtt ctttggccac ttcttgtaac acttttaatt cattcttgtc
     3361 gatattggct cgctcgttct tgccaaagcc gtacagaaaa aaccagcgat ctgccatctt
     3421 cgtggcgaca atcactctca cgccacctcg cttgccctga cccgataacc cgattcgctt
     3481 cttcactacg ttgccaccga gatcggcatc gatgagacct tgagacattt cgaagactgc
     3541 atgacaaatg gcatcattgg gcaagcctac ctttcgcatc cagcggctga aagttcgagt
     3601 cagaaatact cttctcatca aaaaaagtat atcacctggt acaacagatt tctagttaag
     3661 tttttagcga tcaagtttgc gttgtcttgt aacggcacgc gcgattagtt tgaatatttg
     3721 ttcttagcta tcggcgctta accttgactg aagtaaataa aattataccc tttggcgcac
     3781 agtgaacagt agcatagtga taacctaatt ttgagtacgt acgttttcca ctcaaccctg
     3841 gatagttttg catgctgatt gacacagaaa gccaaataat caatgatatt cagctttgct
     3901 tcttcctttg tcttgaattt ctcgtagtac gttaggttgg gctgccgaag aaagcccaac
     3961 acgcagcagt caagacctaa cgatttaccc gaacatatcc gttttcgcat gtccaatagt
     4021 aaatccctta caataagttc gtttgtcaca tttgcctaaa aataacaaca tgactttgga
     4081 ttctaatatt cagcgcgcgc ccggcaacgc cggttggcgg ctcgataaca gttacgcacg
     4141 gctgcccgat tatttttatg cgcaattgag cccggtgccg gtgcgtgcgc cgcaggccgt
     4201 gctactgaat cacgcattgg cggaatcgtt agggttgggt ttgcaggcgt tatccgcgga
     4261 ggaaggggcg gctttgtttg ccggtaacat cctgcccgaa ggtgcacaac cgatcgcgca
     4321 agcctacgcc gggcatcaat tcggtcattt caacatgttg ggtgacggcc gcgcaatctt
     4381 gctcggtgag catctaacac cggacggcag acgtgtcgac attcaactca aaggctccgg
     4441 gcagactccg ttttcccgcc gcggtgatgg ccgtgcagcg ctggcaccga tgctgcgcga
     4501 atacatcatc agcgaggcaa tgcacgcgct cggcattccg tcgacgcgga gcttggcggt
     4561 ggtcactacc ggcgaacaag tccagcggga aacaatgttg cccggcgcga tactgacgcg
     4621 cgtcgccgcc agccacattc gcgtcgggac gttcgagtac gcagccggac acggcggcaa
     4681 ggaggcggtg cagattctgg cggattacac cattcagcgt cattacccgg aattgcagaa
     4741 cagcgacaac ccctatttcg cgctgttaaa tcgcgttatc gagcggcaag cagcgctggt
     4801 tgcccaatgg tcgatggtgg gtttcattca cggtgtaatg aacaccgata acaccagcat
     4861 cagcggtgaa accatcgact acggtccgtg tgctttcatg gatgcataca atccggatac
     4921 cgtgttcagc tcgatcgacc agaacggccg ttaccgctat cgtcaccaac cgatgatgat
     4981 gcaatggaac ctcgcgcgct ttgccgagac gttattgccg ttgttcgatc cgtcgcagca
     5041 acaagccgtg gtgatggcgg aagaaagcat tcgcgcgttt ccgggaattt accaaacgca
     5101 ttggatcgac ggcatgcgca agaaattggg attattcacg gcggaagaaa acgacagtga
     5161 gctgattgcg ggtctgctcg actggatgac tcaacaccaa actgattaca ccaacacctt
     5221 ccgcgcgctg accgaaccgg cattgccgca agatgcgatg ttccgcgaaa gcagttttat
     5281 ggaatggcat agccgctggc agcaacggct ggcgcgtcaa ccacaaccga tatccgaatc
     5341 aatgcaattg atgcaggcga acaatccggt cattattcca cgtaaccacc gtgtcgagga
     5401 agcactgaca gcggcaacgg aacagggtga ttacactgtt ttgcagcgct tgctcgccgt
     5461 acttgcacaa ccttatgaag ataagccgga gtactgcgac taccgcattc cacccgcgcc
     5521 atccgagcgc gtttatcaaa cgttttgcgg gacgtaatgc tgccgcatct cactgtcaga
     5581 aaggagaact accatgtcca tcaccttgaa tcacactatc gtgccgagtt ttgataaagt
     5641 cgagtcggca aaattttata gccgggtgtt cggttttgag tacgtcggcg cgttttcgca
     5701 ttttatcgtg gtgcgcgtta acgatacgtt gtgcctggat tttgacaatg aaacgcaatt
     5761 tgagtcacat cactacgcgt tcaaagtttc ggagcgagaa ttcgacgata ttttcgcgcg
     5821 attgctggcg gaaaaaattc cgtacggcag cggccccggc agtgccaacg atatggcgat
     5881 caatcacaac tatggcggcc gtggcgtgta tttctgcgac ccaaaccggc acttactgga
     5941 aatgttgacg gcggactacg tgatgccacc gcaataacgg gcaagatttt gatagttaaa
     6001 gaaactgcta acgttacctt atcgattttg gagagatcaa ctgatgttaa gtcaaagtag
     6061 tactttaggg cgctacaacc tgtcgatgtg ttgtagttca tggttctttg cgaagtgact
     6121 gggtgtaaac gtgatattta ttctttcaga agatagcgtg tagcagcatt ggttgataaa
     6181 cgcagtatag gatggatgag caagataatg gggtaaggca tcttcgtggg aaagttgaat
     6241 gtgccaacac agttgatagc gcgtcggttc tgtgatggct ttacctaaaa acacattgcg
     6301 tacactgggg attttgttta acaatgcgct accttgagcg gaaagcaaat caatattctg
     6361 cggttggttg ggattgaata agataatttg ttctactgaa ttccagtgcc gacattgaat
     6421 gagtacttca gcagctctgc ctgccgatcc ccagaattgc atgtattgtt tgattcgctc
     6481 aaataatcga tcatgcacac ctgtcattgc ttcaatgtat ccattataag attgtgcatt
     6541 cagattggct tgaatctgat gggctgccaa atccgataac tcggtatagc agttgatttt
     6601 agcaattcca ctcaagatta gcttatggta ttgctcttcg actaatccac tgccgccatg
     6661 cagaaccagg ggaattttga gcgtttcatc gatgcgtttt agtcgcttga aatcgagctt
     6721 ggctcgacta ggctgccgtc catgagaggt accgatggaa accgccaaga aatcaacgtg
     6781 agttctggtg acataggctt ccgcttcttc agccgaagtg aaaatgattt cacctaaatg
     6841 ggagatcgca ttttcacctt cacttccgcc gacaaaaccc aaagcgcctt ctacggcaac
     6901 gccacagcca tgtgccattt cagtgacacg agtcgtttgt gtggcattgg ccggaaatgt
     6961 gtcttttgaa gcgtccagca tgacgctgtt acagcctaat ttaatcgctt gtacgatcga
     7021 ttcatagctt tggccgtgat cgaaatgaat ggcaaccggt actttggcat gatgtgctgc
     7081 gtgctcgatg gatgacaata tgagttgagt accgaagtgc ttgaataacg gttcggagag
     7141 gttaaaaata actggtgagc gacaatattc agcagcactc aggatcgcat gtagaaaatc
     7201 taagctaact aatccaaaac caccgattgc gtagttattt tggtaagcat ggtctagcat
     7261 atctcgcata tcgacaagtg gcatttcctt tccccgtatt cagagtcagc ctgaatcgct
     7321 aattgtgcct aatgaagtgt gagtttttcg agggtttgct gcaaatcttc taaagccgac
     7381 cccataatca atcccgcaca gctatgatat aaccatagct cgcatgcgat tggtcgatgt
     7441 aattgatttc gatcttgtgt tctgaattct cgccaagcga gtacgggttg atcatctgca
     7501 aagctatcca cacataacca aatggtgtta tttaaaatca cttctagtcc acggtggtgc
     7561 tcaagcgcta ttcgtagcgg ttggcctatt cttaagatag caagacgtcc ccggttgtag
     7621 cactttgcat ttattttctt agggaattta cgtaatggaa gtgcaagcgt atcggcttct
     7681 atcatcgcaa tcctcatttt tgttagttat ctaattctga ttagactttt aaatcttgct
     7741 gctaccagta tcgagctttc cttctaaaaa tacacgactg gaccggatat cttcttcttc
     7801 aatggtcatt aaatcaatac tggttaaggt ttttttcgca ctggtaaaca gtcgattggc
     7861 ttgcctcaac ctggcgcgat ctaatgcatt gcgaacagaa cgtgcattgg caaagtgttc
     7921 gagtttcatc cgtagctgga gatactcgct aaaagcgttt tctcctgatg ggctgaagcg
     7981 gtaattctgg gcggccagca ttaatttagc aattgctaac agctcattgg cactgtaatc
     8041 ggggaaatca atgtgatggg cgatgcgcga acgcataccg gggttactat aaaagaattt
     8101 atccattcga tctttatagc cagcaaggat aacgactaaa tcatcgcgat tgttttccat
     8161 cgtttgcaat aagatttcga tcgattctgc accatagtca cgctcgttct ctggtttata
     8221 cagataatag gcttcatcga taaataacac gccccccatt gccttcttga taacttcttt
     8281 ggttttaggg gctgtatgac cgatatattg tccaactaag tcatcgcgcg ttacggatac
     8341 taagtgacct tcccggacat aattcagtcg atgcaaaatt tcagacatcc gcaatgcgac
     8401 ggttgttttt ccagttcctg ggttgccagt aaaacacata tgtaggcttg gcgcaccagc
     8461 ggataagccc agttgtttgc gaacacgatc aaccagtaga agtgcggcta tctcatgaat
     8521 tcgagtttta actggtttca ggccgatcaa ttcccgatct agtttttcta gaacttcttt
     8581 gatgttcgag tcttgaaatt cagcttctaa atcgactgaa accgtttcgg ttgtcattag
     8641 attttttgtt gtcttagcca cgatgcactc cttgttctcg cattgctaga atcggtttta
     8701 acttatattg gtgagtaaaa gcctaaattt tctctctctt tggataaaga gagagaaagc
     8761 aggtttaggt tagtatcgtt ctccttcagg tttatctgtc gcataagaat gaatggtgta
     8821 gttgatattt ctgctggagc tttcttgtcg catcaagcta aaaccaggtt cattttctgg
     8881 gcggttcaca atataagaca tagtaggact ttctacaccg cgactagagt caaatgctgt
     8941 tactctaatg tagtgattcg gaaacgtttt gcgacagtta ttgatttcca tcaaaatccc
     9001 tgcaggatct tttaaatcaa acattggatt gccaaacatc tcccagtagg tattgcgtgg
     9061 atgagggtca tccgtatatt cgatgccgat tgcccatcct tttttgagtg cgtatttgat
     9121 ttgtgatgag atctgcttgt cagtaagcgg cggcaggaaa gagaattgcc cctgtgttag
     9181 gcgattgcca gtatttgtca tcatgattgc ttctccttgg gctattgact catggttgta
     9241 gaaggaacga aatcggcggt gtcggtagat tcataattga aggtgatatc tttccaagta
     9301 tctaatgcaa gcttaagcgg tgagcaccat tttgctgctt ctgctagaat ctttggtcct
     9361 tccgcaacat aatcacgacc ttcattacgc gccatgatca tcgcttccag cgcaacgcga
     9421 tttgcggttg ctcctgcttg aataccttgt ggatggccga tggttcctcc accaaattgc
     9481 agcactacgt cttcacccag gtaatcaatg agttgatgca tttgacctgc atgaattcca
     9541 cctgacgcga cgggcataac ttttctgagg gaagcccatc cctgatcgaa gaagattccg
     9601 tgctcaaggt tacgctcaat attggtttcg cggagtgtgt catagaatcc ccggatcatt
     9661 aatggatcac cttctagctt gcctacaaca gttccagcat gaatatgatc tacaccagcc
     9721 atacgcatcc atttacaaat gactctaaaa ttcatgccat gatttttctg gcgcgaataa
     9781 gttgagttac ccgctcgatg taagtgcaaa atcatgtcat ttttacgcgc ccatttcgcc
     9841 atggattgaa tcgcggtata gccgacgacc aaatcaatca taacgatgac tgatccgagc
     9901 gatttggcga attctgctcg ttcgtacata tcttccatgg tggctgcagt tacattgaga
     9961 taatgccctt ttacctctcc cgtggcagca gcagctttgt taaccgcttc catacagaat
    10021 aagaagcgat cacgccagtg catgaagggt tgtgaattaa tgttttcatc atccttcatg
    10081 aaatcaagac caccttttag gccttcataa acgacacgtc cataattgcg accagaaagt
    10141 cctaatttag gtttggtggt agcgcctaat agggggcgac caaatttatc cagccgttct
    10201 cgttcaacga caataccggt tgcgggacct tggaatgttt taaggtacgc tacggggata
    10261 cgcatatctt ctaaccgcaa tgctttgact gccttgaagc caaatacatt accgataatt
    10321 gatgcagtca aattggcaat ggaaccttct tcaaatagat ctaagtcata ggcaatgtac
    10381 gcaaaatatt gctgatccgt ttttgttcct ggtcctgtat tggggacagg atcaacacga
    10441 taggcttttg ctcgatagag ttcgcaagct gtgagtcgat cagtccagac aaccgtccag
    10501 gttgctgttg acgattcacc ggcaactgcc gcagcagctt cttctggttc tactccttct
    10561 tgtggggtta ttcgaaatag tgcaatgacg tcagtatctt ggggaatgta atcgggttcc
    10621 caatagccca tttttttgta cgggatcacg cctgatttat accgttcttt accttcttta
    10681 atcgtttcag aagccatatt tttctccaag gtgttaaaag gatgtggcca cacgatgagt
    10741 cataggaatt agcgcattgt gggttggaat tatggtagat ttttattacc ataaataaaa
    10801 atcaattatt tctatagtaa tgatagatat aaatctatgg tgacagtttt ttaagaggga
    10861 taaaacgatg cgtcacagca caatacgcca gcttgaggtg tttgaagcca ttgcacgttt
    10921 gaaaagcttt actcgtgcag ctgaagaatt atttctgacc cagccaacgg tatcgatgca
    10981 aatcaagaaa ttaaccgatg atattggttt gcctttattt gagcaggttg gaaagaaaat
    11041 ttatttaacg gatgcaggga aagaacttta tcaaacctgt ttaggcatat ttgaacattt
    11101 tgagcggttt gaaatgatcg catccaatat gaggggccta aaagctggaa agttgaggct
    11161 tgcggttgtg acaacagcaa aatattttac gccgcgctta ttaggtatgt tttgtaaaaa
    11221 atatcccgga attgaggtta agctaaaagt aacgaatcgt gaacgggttt tagagcgatt
    11281 actccataat caggatgatc tttatatttt gggtcaagtt ccggaggaaa tcgatgctgt
    11341 tgctgctcct tttctagata atccattggt tgttctggct tcaacaaacc atcctcttgc
    11401 caaagctaga aatattcctg ttcaatcact gagtgaagaa gcttttatca tgcgagaacc
    11461 aggttctggt acccgtatgg caactgaacg tttttttacc gatcgtgggt taaaaatcaa
    11521 tatcagaatg gaattgggaa gtaacgaggc gatcaaacaa gccattgttg gagagttggg
    11581 tattgctgta ctttcgcgac ataccttggc attagatgcg ccgatggggc agcttgttat
    11641 gttgcatgta gaaggttttc cacttaagcg ctcctggtat tatgcttatc catccggtaa
    11701 acaattatcg attgtggcgg agacttttct tgagtattta cgccaggcac ctgattttct
    11761 ggtcgatttg tcgtgctacc atgcttcagt tggatgctgt ccgttattga ataaaagtaa
    11821 ttaaaatcag atcgcttaaa tcattctaag tactaatcat gaacttaagt gatggttgat
    11881 agtgaactac tcgtgaaaac aagccatata tgacctgatt gactggtaat tgctgcaggc
    11941 caggattaga atgactaaga tagattgact cgatgaattg agagcgcacg tcagtttata
    12001 agggacacac gaatagtgga tataacttgt gatgcaaaat acagtacaaa aaatcgaaat
    12061 ttatacagat ggagcttgta aaggtaaccc cggggttggc ggttggggtg ccttggttat
    12121 gtacgatggg catactcaag agttgtttgg tggcgaagtg agcacgacaa ataatcaaat
    12181 ggagttgatg gcggcgattc gcgctttaca agcgttagaa tcattaccgc tagctgagtc
    12241 ggctcgggtt catctgttta ctgattcaca atatgtacaa aaggggatta gtgaatggat
    12301 cgagaattgg aagaaacgag gatggcggac tgctgaccgc aagccagtta aaaatgaatc
    12361 attgtggaaa acacttgatc aattatcgca gcgctattca attgaatggt gctgggtaag
    12421 aggccatacc ggtcataatg gcaatgagcg agcagacgca ttggctaatc ggggtgtgga
    12481 aaccgtgatg gttggaaatc attcagactg aagtataaac cagtaatgcg ccaaattttt
    12541 cttgatactg aaacaacggg tctcgatgta aagtcgggtc accgtatcgt ggaaatcgct
    12601 gctgtcgaag tttgtaaccg acaactgacg ggtcgccatt tccattatta tttgaatcct
    12661 gagagagaaa gtgaagccag tgctttacag atccatggat tgactaccga attcttacaa
    12721 gataagccta aatttcctga tatttgcgaa gcgtttattc agtttattga aaatgcagag
    12781 ttattgattc acaacgcacc ttttgacgtg agtttcttga acaatgagtt ggtgttgtgt
    12841 aatcaaaata cactgagtag ctattgcact gagatcatcg atactttgat cttggcgaaa
    12901 gagcttcatc cgggtaaacg caataatctc gatgctttat gtgagcgcta tcaagttgat
    12961 cattcccatc gtgtactgca tggcgcactg ctggattcag aactacttgc tgaagtttat
    13021 ctcaggatga cgcgggggca agatagctta ttaatggatt ttgatacacc tgtcctagat
    13081 atcagtaccg atgaagcact taaaggattg caactcgttg tactgtcggc tgatcctagc
    13141 gaattggaac ggcacgccaa gcgcttggag gcaattaata aggtaagtaa agggaattgc
    13201 ttgtggcagt cgctgaatga ctgattgcta agctaacgat tgtttgactc attttctttt
    13261 ataaggaagt aacttgttat gattcatgaa gttcaagggg atattttgtt aagtaaagcg
    13321 caagtgatca ttcacggtgt cgcaccgaat gatcattttg atagtggatt ggcattgtct
    13381 ctacgagaac aatggccagc tatggttaaa gatttccggc atttttgcca aacgacacat
    13441 cctaagtcag gagcagcatg gctgtggtcg ggtactggag gggtacatat tctcaatttg
    13501 ttgacgcagg aagaagcact gggtcaccaa actaaaccag gcaaagcgag cactgaaaat
    13561 gtaaaccatg cattgcgagc cggtcgacat ataatcgaga aggaaggttt tagaagtgtc
    13621 gctttaccgc gtcttgcaac tggtgtcggt ggtttggatt ggaatagcgt aatgccactc
    13681 atccagaatc atttaggcga tttagctatt ccagtttata tctatgctca gtttattaag
    13741 ggaaagcagg cacttgagcc gagtaaccct tgatgtgaga tagcaatttc aagtgtttcg
    13801 aggggcaaac ataataatgg ccatgccaat taatgcaaca caagccccga ttatgtccca
    13861 tatcgtgggt ttgatgccat cgatagccca aagccagaat attgcgacag caatgtaaac
    13921 acccccgtaa gccgcataaa ctcttcctgc tgcggttggg tgcaatgaga gaagccaggc
    13981 aaagagggca agactaaggg cagcaggaac aagtaaccag atactttttc cttcttttaa
    14041 ccacagatag ggtagataac aaccaacaat ctcagcaacg gcagtgagca agaagattaa
    14101 gatggttttg atttcaatta tcatcagact gattgttatg ctgaatgtgc atcgtttttg
    14161 attgaggttt cactcggcgc atggctgtta aataggcgat gatttcatcg atgacttgat
    14221 cccgatcagg aatatcagcc gctaatgaca acattttaga gtttaatcgg atggccgtag
    14281 gattatcaat ccataatttc aaataggtgg gttgaatata ttctgtcaca ctgaccggat
    14341 aattcaattc agcggctttt ccactacctt gaccattgat agtatggcaa gcgaaacagt
    14401 gtttacggaa gtgcaagaat ccacgctgaa cttcggctga agtatcattg ggtggaaaaa
    14461 ttgcggggaa acgtgctgag aaagtagtga gttcgattcc tatgatttga taaggcatat
    14521 cgacagcacc ttcatactgt aattccttag aacttaaatt gtcccatact aggtaaagcg
    14581 gccccaggtg aactttttca ttattttgaa gtaaattctt taatgtaaaa ggatgattat
    14641 ccgcactggc aaaagcaaaa taagcgttgt attgtagaaa ttttgtgact gggatgcttg
    14701 gctggaactg atcttcacag atgaaaacga tttcttccgc agctctccag tcggatccaa
    14761 agataaaatc aaaaagtgct gtggctgagt aggcctgata tgttcgttct tttttttcgt
    14821 gaacttcaaa tatttttaag gtttgcttag ttaaatagcg ttcaagttcc gccaatggca
    14881 agttccgaat tattttatct ttatgcttaa attcgatcag attctggctt atagtagtct
    14941 gagcaaaact tgaactgacc aggatcaaaa gcaaaataac tgaagtcaga aaactgattg
    15001 gccactgatc gttcattgtt tgttgcagta atcgacaatg cttaaataca atgattgtat
    15061 gcggttgttt ctagattcca tcgcgcttta actgtatatc gatagttaga atgagtttct
    15121 aggtaatttt tatctccact actgattcgc attgcagccg cgaaagcaat cattgcacca
    15181 ttatcagtac acagcgctaa ttcaggaaaa tgaacatttg cacctaattg actggcttca
    15241 agcgtgagcc tttggcgcaa ttgtgaattg gctccgactc ctcctgcgac aacgagatta
    15301 ctcaggctac aatgcttgag ggccgcgagt gatttttcaa ccaaaatatc gacgacggct
    15361 tcttgaaaag cgagggcgat atctgctttg atttgatcgg ttatctcaat atgtttgatt
    15421 agcgtttgaa cagatgtctt tagtccacta aagctaaaat tgaaatcgcc actgtttcgc
    15481 attggccgag gaaaagtaaa tcgtccactt tctccccttt cagccagtaa tgagagtgat
    15541 ttccccccag ggtatcctaa tcctaacagt tttgcggttt tatcaaatgc ttcgccagca
    15601 gcatcatcca atgtttcacc taagagcgca tagtgggcaa ctttatcgac cctgattaat
    15661 tgggtatgac ctcctgatac aagtagcgcg acaaaaggga aggtaattgt tggattagtg
    15721 agtaaaggag atagcaaatg tccttcgaga tggtgaattt ctaaaatagg aatacgtaaa
    15781 gcgaatgcca gcgcatttgc aatacttgca ccgactagaa gcgcaccagc aaggcctggg
    15841 ccttgagtat aggcgatggc atttattgct ttgagatcga tttgagcgat cgctaaattt
    15901 tgttttatta aaggtagaac ccgattgata tggtcgcgag aagctaattc tgggactact
    15961 cctccatact cattatgcat ttctatttga gaatatagtg tgtgacttag taagcctttt
    16021 tctgtatgga aaagagcaat accagtttcg tcacatgatg tttctatccc cagaacaatc
    16081 atttgctagt ttttgcgtaa taaaagagaa catcagttaa cttgaacgat gattcgaatc
    16141 ataagttaca gagggttaat tcgaattcaa tatcatcttc gtaaacttta actttctgtt
    16201 cgggtgagaa aggtataaag cgaatactca atgcatactt attagcgcta atttctggta
    16261 cacaaggcaa attttcatca attcggagtc tgatcatgtg tgccatgtga tcagagcctg
    16321 tttgctgaaa ttgtccttgc ttagcggtgt aatggtagga tcttccacta tttctcagta
    16381 acatcaatac aattttaaag ccttcacgga tcgggataag aggttctatc catactttaa
    16441 agtcttgatg acgatgttcg ggtgataaac ttagccaata atggtaggaa ggtaaatcaa
    16501 actcgcaggc gccaccggga atactcattc gttgcttgat actcattaac cattcattat
    16561 cccgcagatg gccgcctatt tttccaggaa tattgagtag atctctaaat gtgactttga
    16621 tatcagttaa aacacgattc aaagcgtctt ccgaaacatc aggattttta cgtaaacttt
    16681 ccaacatggt tctttgtttt tctagctctt ggagtagatc cgatttaagg tcagctcgac
    16741 ttgtaacatc cagaatttcg aataatgcta ctaatgctga atgatgttca gtcggtgtat
    16801 tacatttaga aaagaaatct actttgctga ataaattctc gagccgcaat aatgttcgga
    16861 ctctctcatt gagggggtgt tcgtagcaaa tcacgttaat tttatatgca tcagtttatg
    16921 acaattcgaa tcatacctta tgatttatta ttactaaatc aaagttttgt aatttacgaa
    16981 aaaaagcttc aacgatctca aataataata gggaaatgcg tttcgccatc ccttttggag
    17041 gggaaattcc catcggtaat accctgttac tttctatggg gcgtcatttt tttatatgcc
    17101 tctaaactat tttcagttta atctgggtgt gactcaaaca ggcatt
//
LOCUS       DAOQKQ010000038        16917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_1537_length_16917_cov_6.741015, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000038 DAOQKQ010000000
VERSION     DAOQKQ010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 16917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16917
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1537_length_16917_cov_6.741015"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..1111)
                     /locus_tag="PKZ49_03950"
                     /pseudo
     CDS             complement(53..1111)
                     /locus_tag="PKZ49_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611796.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            1346..1663
                     /gene="flhD"
                     /locus_tag="PKZ49_03955"
     CDS             1346..1663
                     /gene="flhD"
                     /locus_tag="PKZ49_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HND35747.1"
                     /translation="MEATQILEEIRDINLSYMLLAQQLLREDKISAMYRLGINEDVAN
                     ILEKLTSSQLLRMADTNILLCRFRFDNNLIAEMLSNHNKDRALSQSHASIIISSLPAE
                     TIT"
     gene            1681..2244
                     /gene="flhC"
                     /locus_tag="PKZ49_03960"
     CDS             1681..2244
                     /gene="flhC"
                     /locus_tag="PKZ49_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HND35748.1"
                     /translation="MREKSLLSEAKQIQLAIELIQLGARLQVLEAHTKLSRERLVRLY
                     KEIKGISPPKGMLPYSEDWFMSWQPNIHSSLFINIYNYITKYANINSIDAIIKSYKLY
                     LEHIGIYDIQRVLSLTRAWTLVRFLESKVLSITSCTSCKGHFVVHSLEINSNHICGLC
                     NIPSRAGKTKKDKLKNDLLGTKHQHAA"
     gene            complement(2438..3250)
                     /locus_tag="PKZ49_03965"
     CDS             complement(2438..3250)
                     /locus_tag="PKZ49_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35749.1"
                     /translation="MKYSARTPVTIISFLFLLMLTGMSKIVLSGPVLYVHDNAGKLAT
                     LDATTGSIDIIGDMGITMTDIAFSPTGDLYGISFGRLYAIDSDTAQATLIGSHAVPGG
                     NTLVFGEDGTLYSAGYMSTSLYTIDVSNGATTNLGSMGFFAGGDLAFHNGHLYMASLS
                     NKLVDIDLDDLSNTVAIGNFAIGGVFGLATGSDNNLYAVANTAVFSVNTTTGGVIDLV
                     SFAKQGLGQAFGLTSFIPDDIVIAAEIAEPTTIMLLLSGLLGFRRFSKSGQK"
     gene            3689..5518
                     /gene="ftsH"
                     /locus_tag="PKZ49_03970"
     CDS             3689..5518
                     /gene="ftsH"
                     /locus_tag="PKZ49_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HND35750.1"
                     /translation="MDNNEVNKKLQFNFWYIVIAILGMLFLQNLYTQYTRIEPIPYSQ
                     FQDLLEREKIAEVAITENQIYGTLKASEPNGMKEFVATRVEPELAEILEKHNVIYTGV
                     IRSTWLRDLLSWIVPMLLFVGIWLFIMRRVSGGMSSGLMSIGKSRAKVYVETETKVTF
                     ADVAGVDEAKEELIEIVNFLKNPADYSRLGGRAPKGILLVGPPGTGKTLLARAVAGEA
                     GVPFFSISGSEFVEMFVGVGAARVRDLFEQARQMAPAIIFIDELDSLGRARGAYGLGG
                     HDEKEQTLNQLLAELDGFDSSSGIVLLAATNRPEILDAALLRAGRFDRQVLIDRPDKT
                     GREQILAVHLKKVKLGVDVMPDQIAALTPGFTGADLANLINEAALLATRRKATSVSMD
                     DFNNAIERIIAGLEKRNRLLNLNERKIVAFHELGHAMVALALPGTDEIHKVSIIPRGV
                     GALGYTIQRPTEDRFLMTKPELENKMAVLLGGRAAEQVIFNQVSTGASDDLVRATDIA
                     RAMVLRYGMSEALGHVAYEREHSAFLQQGYALPQSREYSEATANKIDEAVRLLVDQAL
                     ERAISILRKNQTLLEQTAIDLLKAETLTQPNIAVLKQALIRET"
     gene            complement(5612..6133)
                     /locus_tag="PKZ49_03975"
     CDS             complement(5612..6133)
                     /locus_tag="PKZ49_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HND35751.1"
                     /translation="MKLHYKIATLACALLPFYAFAGGNPDHVKLPGNYQQIFTQYDKS
                     NRANQTQVAKFFANEIAVDSYKRGEEAAPGSIVVMEIYEPRKDTEGKIIAASTGIFEI
                     EKLVAVAVMEKRADWDKSFSPDDRTGNWGFAVYSPDGVAKSNDLNCVQCHTPLKDQDY
                     LFSYGKLVNFTKK"
     gene            complement(6285..7040)
                     /locus_tag="PKZ49_03980"
     CDS             complement(6285..7040)
                     /locus_tag="PKZ49_03980"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HND35752.1"
                     /translation="MKIRVTRVQHQNNPTHCIVLNNQTITYSLVRRNRKTFGLRINAS
                     GLTISAPLRIPQREIEAILQRKADWIIEKLDAWENSSSNHLEWRLHAVYWLLGEPYQL
                     IALSAQLQLVPQNSIKPDMVRPCSAESSSTQFSAQQLEHFVLAWYQQQATACMTDRLA
                     FYAEKLGVGMPVFHLSNARSRWGSCNASGVIRLNWRLIQLPLALIDYVIAHELAHLFE
                     MNHSRAFWEVVAKIYPEYRRAQRELKQIGKLMI"
     gene            complement(6976..7779)
                     /locus_tag="PKZ49_03985"
     CDS             complement(6976..7779)
                     /locus_tag="PKZ49_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HND35753.1"
                     /translation="MSRMLAILRSLVYTVLQIVITPPYAIFTLSCFWLPPHQRYQVTY
                     GWTRIMLFLLKTICGLHYRIIGAEHIPKQPSIVLSKHQSAWETLAFQQIFPPQVWVLK
                     KELLRIPFFGWGLAMTSPIAIDRGSGKKALQQIVDQGKDRLAQQFWIVIFPEGTRIPP
                     GHIGKYHIGGAWLATHTGALVVPVAHNAGEFWGKNSFVKNPGTITVSIGKPIDPAGLE
                     AGELNDQVKAWIEAEVSRISQFKKIEKQNEDSGNTCSTSKQSNALHRSK"
     gene            complement(7776..8309)
                     /gene="gmhB"
                     /locus_tag="PKZ49_03990"
     CDS             complement(7776..8309)
                     /gene="gmhB"
                     /locus_tag="PKZ49_03990"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HND35754.1"
                     /translation="MKLIVLDDKGVINEINDPFIQTPEQWVPIPGSLEAIAKLTHQGY
                     RVVVATNQSGIGRGLLEMDTFNAINDKLFKAVQAAGGYLDALFFCPHAQQEHCHCRKP
                     QIGLFKEIIQRYSTDLKDVPVVGDSLKDLQAASSIGATPILVLTGNGQQTQATNNLPT
                     GTQVYPDLASVANALLK"
     gene            complement(8311..10488)
                     /gene="glyS"
                     /locus_tag="PKZ49_03995"
     CDS             complement(8311..10488)
                     /gene="glyS"
                     /locus_tag="PKZ49_03995"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HND35755.1"
                     /translation="METRNLLIELLVEELPPKSLKKLGESFARALSQALAMQQLTSFN
                     TIITPYATPRRLAVHLTEVLSQSPNQQQSHKLMPVAIGLDSAGQATPALIKKMSSLGM
                     EIFSVAELKRAMDGKAEALFLERTVTGTLLVNGLQTALEQAIAQLPIPKVMAYQLADG
                     WSTVNFVRPVHSLIALHGKEVVAINLLGLTAGNTTRGHRFEAKSNSIVIQEADSYAQQ
                     LQNEGAIVASFVERRQAISDQLIATANKVNLNLIADEALLDEVTALVEHPNVLMGQFA
                     EAFLEVPQECLILTMKANQKYFPLFDQHGKLTNQFLLVSNICPDDPHAIIDGNERVVR
                     SRLADAKFFFDQDRKKTLILRLPGLNAVIYHHQLGTQGLRIEYLCALASKIGAMIGGD
                     QLAIEAAQATKLAKADLLTDMVGEFPELQGVMGRYYAQHEGLSDAIAYAIEDHYKPRY
                     AGDVLPRNLIGVCVALADKLETLVNLFAIGQIPTGDKDPYALRRHALGVIRMLIEKDL
                     PIELNKLLQLVFDIVQDEAIAQQWQQAMVDEQRSVVIPKRPILTTGIVESLQQFISDR
                     LAGVLREQGYTAQEVDAVLALNPQHLNDIPKRLAAVRAFSSLPEAASLAAANKRVSNI
                     LKKVEHEISDRVNSNQLCEEAEKALHEALLDLTDKVDQAFNEKDYVVTLQILAELKTV
                     IDAFFDHVMVNVEDGDLRANRLALLKQLQQIMNRVADISRLAS"
     gene            complement(10491..11408)
                     /gene="glyQ"
                     /locus_tag="PKZ49_04000"
     CDS             complement(10491..11408)
                     /gene="glyQ"
                     /locus_tag="PKZ49_04000"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HND35756.1"
                     /translation="MTPLTFQEMILALQNYWSRQGCALLQPYDMEVGAGTSHTATFLR
                     AIGPEPWRAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVVLKPAPQTILDLYLGSLQEL
                     GFDLTCNDVRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGINCKPITGEI
                     TYGLERLAMYLQGVENVFDLVWTKGLTYRDVYHQNEVEQSIYNFEQSDTAFLLLAFGK
                     YEQQANHLMENQLPLPAYEQVLKAAHSFNLLDARGAISVTERAAYIGRIRNLARRVAQ
                     AYYDSRERLDPPFPMAPREWVEQMSCKAG"
     gene            complement(11491..12993)
                     /gene="ppx"
                     /locus_tag="PKZ49_04005"
     CDS             complement(11491..12993)
                     /gene="ppx"
                     /locus_tag="PKZ49_04005"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HND35757.1"
                     /translation="MREYSTLAAVDLGSNSFHLQVARVVDKQLYPLDSLKEMIRLAGG
                     ITADKKLDESSQERALDCLKRFGERLRGFPTHAVRVVGTNSLRVAKNAAAFLRKAEGA
                     LGFPIEIIAGHEEARLIYLGVAHALPASDNNRLVIDIGGGSTEFIIGHQLNPYKLESL
                     YMGCVSHSLHHFPDGKLTKSNMKRAELAAQTEIQSIKADFSSRHWQEAYGSSGTARAL
                     GRILMLNQFGDSLSTEEITLKGLEQFREYLLKVGDLKKVNLEGLHSDRTPVIAGGLAI
                     MSAAFTELGIDRMYLTTGALRQGVLYDLLGRSYNDDMREVSVKQFMQRYLIDTAQANR
                     IELLAGELGKQLLSDFSDEEEVEDALQLLSWAARLHEVGISVAHSSYHKHSSYILGNA
                     DMPGFSNMEQAQLSELVLSHQGSLTKVSEFLTNPINLAQSIALRLATLFYRSRMNIEL
                     PQIKLEIKGHQCRLIISQVWLDQNPLTETLLQAEIDAWKKVDWDFRIISA"
     gene            complement(13045..13752)
                     /gene="phoU"
                     /locus_tag="PKZ49_04010"
     CDS             complement(13045..13752)
                     /gene="phoU"
                     /locus_tag="PKZ49_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HND35758.1"
                     /translation="MVNKEHISKRFDADLEEVRTRVLQMGGIVEEQIEQALEALIGGN
                     EATIDQVIANDHRVNAMEVSIDEICSQIIARRQPTASDLRMIMTVIKTITDLERIGDE
                     AAKIARMARLIYAADRSHTPRFVEIKHVAEIALDMLHQALDAFARLDLNAAAHIVRQD
                     EQVDEEFRAILRQLITFMMEDPRKISTCLEILFVAKAIERIGDHAKNMSEYVVYMVKG
                     KDVRHVTADEIEREVRG"
     gene            complement(13833..14492)
                     /gene="rpiA"
                     /locus_tag="PKZ49_04015"
     CDS             complement(13833..14492)
                     /gene="rpiA"
                     /locus_tag="PKZ49_04015"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HND35759.1"
                     /translation="MTQDEQKRAAAAAAIQYVPVGSIVGVGTGSTANYFIDELAKIKH
                     KIEGAIASSNVTAQRLKDHGIEVLDLNTVTDLPVYIDGADEITKHLHMIKGGGGALTR
                     EKIVSAVARKFICITDQSKLVNVLGTFPLPIEVIPMARSYVAREITYLGGHPALRQDF
                     VTDNGNIILDVHGLQILNPVELETTLNQITGVVTNGLFARKAANILLLGTDHGVEMIT
                     V"
     gene            14660..16453
                     /gene="mutL"
                     /locus_tag="PKZ49_04020"
     CDS             14660..16453
                     /gene="mutL"
                     /locus_tag="PKZ49_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HND35760.1"
                     /translation="MNVIKLLPDELINQIAAGEVIERPASVLKEVLENCVDAGATEID
                     IHLMQGGLKLIRVTDNGVGISKEDLPYALTRHATSKISNQEDLQKIVSLGFRGEALAS
                     IASVSRLSLTSFQVGNQHAWEIQAEGSQTIALQPAAGSLGTSLEVRDLFFNIPARRKF
                     LKSESTEFAHCEAIFQRIALSNPTITFNLYHNGKLRSHLASADFPERIKQLLGQEFRQ
                     TALFITEEAADIQLQGLISRPTFSRTTREMQYFFVNGRFVKDKLLNHAIREAYRDVLH
                     LDRHPAYVIYLHINPDNVDVNVHPTKIEVRFRDSRAVHQFIFHAIHKALANGHTESSE
                     ADLPDSKHHIGVVTRNIKKIPLAYSQNRQVNLPLQSAAQPSVFYQTLFGEKSHEIIAA
                     PDLAVQSESHASNFLGYALGQLLGIYILAQSNQGLIIVDMHAAHERIVYEKLKQALDQ
                     QALPTQSLLIPHLLQADALDITTVEENALLLNQLGFEISITSPTTLAIRTMPSILKNA
                     DISKLISKLLEDIRSYGSSQLLTGNRNEILATMACHSAIRANELLTIEEMNVLLREME
                     KTERSDQCNHGRPTWLALSLEQIDKLFMRGN"
     gene            complement(16508..16696)
                     /locus_tag="PKZ49_04025"
     CDS             complement(16508..16696)
                     /locus_tag="PKZ49_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35761.1"
                     /translation="MKIKCFFTILTCAVLVACGKPSAPETPNPEEYGKTIQPFHQIPA
                     DAQEGGGKARSVEEKSSY"
BASE COUNT         4848 a         3887 c         3401 g         4781 t
ORIGIN      
        1 tctcttcctc ctcctctcaa atcattttat ctccggaaaa cccggggcga ttcagctggg
       61 cgggcaagct cgtcactgtt tttttcagtg cactagtcat tgtaacagct ccgtagcccg
      121 caagcgcggg accattcttc gttctgggag tcagcccata gccttcctcg cgaggcagat
      181 ggataagcat ggtaaatcgg cttgagcgct cgaccaatgt cccaattgca gatcgattca
      241 gaccgatgat caagtcacct tcccaatgcc caggtaaggc gcgatcttcc gcctcagcag
      301 ggcggctgga gatcatgaca ttctcgttga catgagccca tgctttagcc tgcgccctgg
      361 ctctcggtac gcgcaacgct cgccctgtgc gcagacaact caccaactcg cgcttgagag
      421 caccgcgacc ttgaatgtag agagcctgat aaatggcttc gtgagaaatg cgcatggatt
      481 tgtcctctgg aaagtcaatc tgtagccgat tggcaatctg ttcaggcgac cagcctttga
      541 cccaattacg atcaccacga tgtggcttat ttcgtccttt gaacggtgcc tgtcgaggtc
      601 cctcaatttt acgcccatca gcgtcataaa cctttccctc caagcaatct tgaacatatt
      661 ggcgcagtcg agggttagtc accagtttcg ccgctttcgg tctcctggca accaattccg
      721 ctttccactg cgcgactgaa gcgcgatatt cgagccgacc gctacgagtg gcagcattgc
      781 gagtcagctc ccgtgaaatt gtcgacggac ttcgtccaag acggcgagca atctcacgca
      841 cgccaatact ttgtgcccgg agcagtccaa tctcttctcg ttcagcaaat gacaaatatc
      901 ttccggatat gtgattagac atgaataata gcatcccgcc tcgataacgg aaccagcggg
      961 tacctaccgc tgacgatacg ccaacagcct ctgccgcttt ctcgcttgtt atcccggttg
     1021 caatctgctt ccaaaatagc cgctcaatct caccgcgaag tgacggcgca cctggcgatc
     1081 gcatcgtcca tctccccgtc aacttttgca tccatcctgc tggtcgcccc ataaacacct
     1141 ccatgatcaa aagtgttgcg acaaccggtt gaatccacca gcttagcttt caaacaacgc
     1201 ttgtcatgta ggaaaaattc ttacaaagat tctagctaaa taattacgca ttattcgggc
     1261 aatatgttat ttacttaaaa tggcctaggg taaagaatac acctactgta attcagatta
     1321 aatattttct ttgtggagac aacgaatgga agccacccaa attcttgaag agattagaga
     1381 tattaatctc agttacatgc ttctggcaca gcaattatta agagaggata aaatctctgc
     1441 aatgtatcgg ttaggaatta atgaagacgt cgcgaatatt cttgagaaat tgacttcgag
     1501 tcagttactt agaatggctg ataccaacat tttactctgt cgttttagat ttgataataa
     1561 cctaattgca gaaatgttat ctaatcataa taaagatcga gcattgtcgc agtcacatgc
     1621 atcaataatt atttctagct tacctgctga aacaattacc taaattaaag gattaagaaa
     1681 atgcgcgaga aaagtctatt atctgaagca aaacaaattc agctagccat cgaacttatt
     1741 cagttaggcg ctagactaca agttttagag gcacatacaa agcttagtag agagcggctt
     1801 gtaaggctat acaaagaaat caagggaatc tctcctccca aaggcatgct cccttattca
     1861 gaagattggt tcatgagctg gcagcctaat attcactcat cccttttcat taacatatat
     1921 aactacataa caaaatatgc gaacataaac agcatagatg ccatcattaa aagctacaag
     1981 ctttatctag aacatattgg tatctatgat atccagcgag ttttgagttt aactcgcgcg
     2041 tggacacttg ttaggtttct tgaaagtaaa gtactttcaa taacatcatg taccagctgc
     2101 aagggtcatt ttgtagttca cagtttagaa attaattcaa atcatatttg tggactatgt
     2161 aatatcccat cccgcgccgg caaaactaaa aaagataagc ttaaaaacga tttactgggt
     2221 actaagcatc agcatgcagc ctaaccaccc aatctaatgg aagcttcctg taacaaagca
     2281 tgagtaccta gaggctatat ttgtagcatg aattgcctca ggtgctggaa tgggattgag
     2341 tagaaaaaat ttaatctcga aatttccgag tcgctagcgc tactttattt taattcagct
     2401 taatatttcc tcttacatta ttgatggaaa tcttggttca tttttgaccg gatttactaa
     2461 atcttctaaa accaagtaat ccggataaga gcagcataat cgttgtgggt tcagcaattt
     2521 cagctgcaat aacaatgtcg tcaggaataa aacttgttaa cccaaaagct tgtcctaatc
     2581 cctgcttcgc aaagctcact agatcaataa caccccctgt agtggtgtta acagaaaaaa
     2641 ctgctgtgtt agcaacggca tacaaattgt tatcactccc agtcgctaaa ccaaaaactc
     2701 caccaatagc aaaattacca atcgcaaccg tattcgataa atcgtctaag tcgatatcaa
     2761 cgagtttatt gctcaaagat gccatgtaaa ggtggccgtt atgaaaagct aaatcgcctc
     2821 ctgcaaaaaa acccatgctg ccaagattag tcgttgcacc attacttaca tcgattgtat
     2881 aaagtgatgt actcatataa ccagcagaat agagcgtccc atcctcaccg aatacaaggg
     2941 tatttccgcc tggaactgca tgactgccaa tcagagtggc ttgcgccgta tctgaatcga
     3001 tagcgtataa acgtccaaag gaaattccat acaaatcacc tgtcggacta aacgcgatat
     3061 ccgtcattgt gatgcccata tccccgataa tatcgataga tcccgttgtt gcatcaagcg
     3121 tcgccaattt accagcgtta tcgtgtacgt ataatactgg gccactgagt acaatcttgc
     3181 tcataccagt aagcatcagt aaaaataaga aactgataat tgttacggga gttcttgcgc
     3241 tatatttcat atcaatcctt aatgcttaac aaagctttta gttaaaacgt tgatctattt
     3301 cttgctatct aatctttact atttgtgaaa actagaaata agtacgtatc aatctttaag
     3361 cagatctcat gccaaaatca aataatagat ataagattat gtatttaaac aattttttac
     3421 tgtcgcttac gcatcaagat aaaataatct aaataatttg ttaagttaat cgacgcaaga
     3481 ggagtcatgg taaagcaaca cacaattcca attcattgaa taaaaaggag atatctgatc
     3541 tatcccatat gtaaaatctt tagttattaa ccgtaaagta aaaaaataca ttttcttggt
     3601 gtggaatgat tgaatttaca attaccgtac gaattcaagt agccctgatc aaatttggac
     3661 taaatcaatc gaaagaagga atattcatat ggataacaat gaagtaaata agaaactcca
     3721 gttcaatttt tggtatatcg tcattgccat cctcggtatg ttatttctac aaaatcttta
     3781 tacacaatac acgcgcattg agcccattcc ttatagccaa ttccaagatt tactagagcg
     3841 agaaaaaatt gcagaggtgg ctattactga aaatcagatt tatggcacat taaaagcatc
     3901 ggaacccaat gggatgaaag agtttgttgc cactcgagtc gaacctgaac tagcagaaat
     3961 tctggaaaaa cataatgtaa tttacacggg cgttatcaga agtacctggc tacgcgactt
     4021 actctcatgg attgtgccta tgttactgtt tgttggtatc tggcttttta tcatgcgccg
     4081 cgttagtggc gggatgagta gtggtttaat gtcgatagga aaaagtcgag cgaaagttta
     4141 tgtggagact gaaaccaaag taacttttgc ggatgtcgct ggcgttgatg aagcgaaaga
     4201 agaattgatc gagattgtca attttctaaa aaaccccgct gattatagtc gactcggtgg
     4261 acgtgcacct aaaggcattc tattggtcgg tccccctggc actgggaaaa cattacttgc
     4321 acgagccgtc gcgggagaag cgggcgttcc ttttttttca atctccggtt cagaatttgt
     4381 cgagatgttc gtcggcgtgg gtgctgcacg agtacgcgat ttatttgagc aagcacgaca
     4441 aatggcgcca gctattattt ttatcgatga actggattca ttgggcaggg cacgcggtgc
     4501 ctatgggctc ggtgggcacg atgaaaaaga gcaaacgctt aaccaattac tggctgaatt
     4561 agatggtttt gattcatcga gtggtatcgt attgttagca gccaccaatc ggcccgaaat
     4621 tcttgatgct gcattactga gagcagggcg atttgaccga caagtattaa ttgatcggcc
     4681 cgataaaacg ggaagagagc aaatcttggc tgtacacctc aagaaagtaa agctcggtgt
     4741 ggatgtcatg cccgatcaaa ttgcagcctt aacaccaggc tttactggcg ctgatttggc
     4801 taatttaatc aatgaagctg cattattggc aactcgccgc aaagctactt ccgttagcat
     4861 ggacgatttc aacaatgcca tcgagcgcat cattgccggc ctcgaaaagc gcaatcgctt
     4921 gttgaatctt aatgaacgta agatcgtcgc atttcacgaa ctagggcacg ctatggtcgc
     4981 gcttgctctg cctggaacgg atgagattca taaagtatcc attattccac gtggcgttgg
     5041 cgcgctcggt tataccatcc aacgcccgac cgaagaccgc tttctcatga caaagcccga
     5101 attagaaaac aaaatggctg tgttactcgg aggacgtgct gctgagcaag ttattttcaa
     5161 tcaggtctca accggtgcct cggatgattt agttcgtgca accgatattg cgcgtgccat
     5221 ggtgttacgc tatggcatga gcgaagcgct aggccatgtt gcttatgaac gagaacattc
     5281 tgcgttttta caacaaggtt atgcgctgcc ccagagtaga gaatatagcg aagcaaccgc
     5341 taataaaatc gacgaagctg ttcgtttgct agtagatcag gcattggaac gagctatcag
     5401 tattttacgt aagaatcaga cgctgctgga gcaaaccgcg atcgacctat tgaaagctga
     5461 aacactgact cagcctaata ttgctgtatt gaagcaagcg cttattcgtg aaacttaagt
     5521 aggattttcg ctgatcgtta tcggacaatc cgctcagtaa gcgactgtcc gatagtgcaa
     5581 gttaaatctg gactgcaatc gagatatttt gttatttctt agtgaaatta actaattttc
     5641 catagctaaa taaataatct tgatccttca atggtgtgtg gcattgcaca caatttagat
     5701 cattactttt agcgactcca tcaggactgt atacagcaaa cccccagtta ccagtacgat
     5761 catcgggaga aaatgattta tcccaatcag cccttttttc catcacagca acagccacta
     5821 gtttctcaat ttcaaatata ccagtactgg cagcgatgat ttttccttct gtatcttttc
     5881 taggttcata aatttccata acaacaattg aacccggcgc agcttcttcc cctcttttat
     5941 agctatcaac cgcaatttca tttgcaaaaa atttagcgac ttgcgtttga ttagcacgat
     6001 tcgatttatc atactgggtg aagatttgct gataatttcc cggtaacttt acatggtcag
     6061 gattcccgcc tgcaaacgca tagaaaggta acagtgcaca agctagagtc gcaattttat
     6121 agtgtagttt catcttctct ccttatcaaa atcaaccaat agttattgtg aaaaatatcc
     6181 caatgctcag actaaagcat aatattgatc tattctttat acaacacggt atttcattat
     6241 ttttgaacga taaagcgata gaaattcacg gtatctttcg atcttcaaat catcagctta
     6301 ccaatttgct ttaattcacg ctgtgcccgg cgatattctg gatagatttt tgctaccact
     6361 tcccagaatg ctcgcgaatg attcatttcg aaaagatgcg ctaattcatg ggcaatcaca
     6421 tagtctatta acgcaagtgg taactgaatc aatcgccagt taaggcgaat cactcctgat
     6481 gcgttgcagc taccccaccg tgaccgggca ttcgaaaggt gaaaaactgg cattcctaca
     6541 ccaagctttt cagcataaaa tgctaagcga tccgtcatgc acgcggtcgc ttgctgttga
     6601 taccatgcta aaacaaagtg ctcaagctgt tgtgcagaaa attgtgttga cgatgactca
     6661 gcgctgcatg gcctgaccat atctggtttg atgctattct gtggcactaa ttgcaattgc
     6721 gccgataaag caatgagctg ataaggttcc cccaataacc aataaaccgc atgaagcctc
     6781 cactcaaggt gattactgct gctattttcc caggcatcaa gtttttcaat aatccaatcg
     6841 gccttgcgct ggagtatggc ttcaatttct ctttggggta ttcttaaagg ggctgaaata
     6901 gttaagccag atgcgttgat tctcaaccca aaggttttcc gattacgtcg aactaatgaa
     6961 taggtaatcg tttgattatt tagaacgatg caatgcgttg gattgttttg atgttgaaca
     7021 cgtgttaccc gaatcttcat tctgcttttc tattttttta aattgactga ttctcgatac
     7081 ctcagcttca atccacgctt ttacttgatc attcaattcg cccgcttcca atccagcagg
     7141 atcgattggt ttgccaatac tcacggtaat cgttccagga tttttaacga atgaattctt
     7201 cccccagaac tcgccagcat tatgtgcaac cggcacaacc aatgcaccgg tatgagtagc
     7261 caaccaagcc ccaccaatat gatatttacc gatgtgacct ggcggaatgc gcgttccttc
     7321 tggaaaaatg acgatccaaa attgttgagc aagccgatct tttccttgat cgacaatttg
     7381 ctgcaacgct tttttcccag agcctcgatc gatggcaatc gggcttgtca ttgcgagtcc
     7441 ccagccaaaa aaagggatac gtagtagctc tttcttaaga acccagactt gaggcggaaa
     7501 gatttgctga aaagctaacg tttcccaagc cgattggtgc ttcgataaca caatgctagg
     7561 ctgcttcgga atgtgctccg caccgataat tcgatagtgc aaaccacaaa tggtcttaag
     7621 taaaaaaagc atgatccggg tccaaccata cgttacttga tagcgctgat gcggtggtaa
     7681 ccagaagcag gacaatgtga aaattgcata aggtggagta atgacaattt gcagcacggt
     7741 ataaaccagc gagcgaagga tagcaagcat acgactcatt tcaataaagc gttcgccacg
     7801 gatgctaaat ccggataaac ttgtgttcca gtcggcagat tattggttgc ttgtgtttgc
     7861 tgtccatttc cagtcagaac gagaatcggt gttgctccaa tgctacttgc agcttgaaga
     7921 tctttcaatg aatcacccac gacgggaaca tctttcaaat cggtgctata gcgttgaata
     7981 atttctttaa ataaacctat ctgaggtttg cggcaatgac aatgctcctg ttgtgcgtgt
     8041 ggacaaaaaa ataaggcatc caggtaaccg cccgctgcct gaaccgcttt aaaaagctta
     8101 tcattgatag cattaaaagt atccatttcg agcaaaccac gcccgattcc ggattgattg
     8161 gtagccacga cgacacgata accctggtgc gtaagttttg cgatagcttc taaacttccg
     8221 ggtattggca cccattgttc gggtgtttgg atgaaaggat cattgatttc attgattact
     8281 cctttatcgt caagcacaat aagcttcata tcaactcgct aaccttgaaa tatccgctac
     8341 acgattcatg atttgttgta actgcttgag taatgcaagt cgattagcac gcaagtcccc
     8401 atcttcgaca ttgaccataa catgatcaaa gaaagcatcg attactgttt ttaattcagc
     8461 aaggatctga agcgttacga cataatcttt ttcattaaat gcctgatcta ctttatcagt
     8521 taaatcgagc agcgcttcat ggagggcttt ttccgcttcc tcgcatagtt gattagaatt
     8581 tactcggtca ctgatttcat gttcaacttt cttgagtatg ttactgaccc gcttattggc
     8641 agcggcaaga ctcgcagcct cgggcaaact tgaaaatgcg cgtacagcag ctaatcgttt
     8701 aggaatatcg ttcaaatgtt gtggattaag tgctaaaaca gcatcaacct cctgagcagt
     8761 ataaccttgt tcacgtaaga cgcctgccaa tcgatccgat atgaactgtt gcaatgattc
     8821 aacgattcca gtcgtcaaaa ttggacgctt aggaatcact actgaacgct gctcatcgac
     8881 catcgcttgt tgccattgtt gcgcaatcgc ctcatcttgt acgatatcaa aaaccaactg
     8941 caacagttta tttaattcaa tgggtaaatc tttttcgatc agcattcgga taacacctag
     9001 cgcatgacgc cttagcgcat aaggatcttt atcgccagtt ggaatctgac caatggcaaa
     9061 caaattaaca agtgtttcta acttatcagc caaggcaaca cacacaccga tcaaattccg
     9121 tggcaacaca tcgccggcat agcgcggttt atagtgatct tctatcgcgt aagcaatggc
     9181 atcactcaac ccttcatgct gtgcataata acgccccatt accccttgca gctcgggaaa
     9241 ttcgccaacc atatcggtaa gtaaatccgc tttggcaagc tttgttgctt gcgcagcttc
     9301 aatcgcgagt tgatcccctc ctatcatcgc gccaatttta ctcgctaatg cacagaggta
     9361 ctcaattcgc aaaccttgag tccccaactg atgatgataa atgacggcat ttagcccagg
     9421 aaggcgtaaa atcagagttt ttttgcgatc ctgatcaaag aaaaattttg catcggccaa
     9481 gcgagaacga actacgcgtt cattgccatc aataatcgca tgaggatcat cggggcagat
     9541 attggaaaca agcaggaatt gattggttag cttgccgtgt tgatcgaata gcggaaaata
     9601 tttttgattc gctttcatgg taagaataag acattcctgc ggaacttcca ggaaagcttc
     9661 agcgaattgc cccatcagca cgttcggatg ttctaccaaa gcagttactt catcgagtaa
     9721 agcttcatca gcaatcaagt ttaaatttac tttatttgca gtagcaataa gctgatcgct
     9781 gatcgcttgg cgacgttcta caaaactagc aacgatagca ccctcatttt gtagctgctg
     9841 cgcatagcta tccgcttctt gaattacaat tgaattggat ttggcttcaa atcgatgccc
     9901 gcgtgtggta tttccggcag tcagtcccaa taaattgatt gctacaactt cctttccgtg
     9961 taacgcaata agtgaatgga cggggcgaac aaaattaacc gtactccaac catcggctag
    10021 ctggtaggcc atcaccttag ggatcggtag ttgtgctatg gcctgctcca atgctgtttg
    10081 caatccattg accagcaatg ttccagttac ggtccgttct agaaacaatg cttccgcttt
    10141 gccgtccatg gcgcgtttaa gttctgcaac actaaaaatt tccatgccta aactgctcat
    10201 ttttttgatc agcgcaggtg tggcttgccc agcagagtct agtcctatag ctactggcat
    10261 aagtttatgc gactgctgtt gattggggga ctgactcaat acttcagtta agtgaactgc
    10321 taatcggcgc ggcgtagcgt agggcgttat aatcgtatta aacgacgtaa gctgctgcat
    10381 agctagcgcc tgacttaagg cgcgggcaaa actttccccc aattttttta acgacttggg
    10441 tggaagctct tctaccagca attcgatgag taaatttctc gtttccatct tcatcctgcc
    10501 ttacacgaca tttgctctac ccactcacga ggcgccattg gaaacggtgg atctaatcgt
    10561 tcacgactat cgtaatatgc ttgtgctacc cgtcgggcca aattgcggat acgaccaata
    10621 taagccgcac gctcagttac ggaaatagca cctcttgcat ccagcaaatt aaagctatgg
    10681 gctgctttaa gtacttgttc ataagcaggt aatggcaatt ggttttccat aagatggttc
    10741 gcttgctgct catatttacc gaatgccagc agcaaaaatg cggtatcgct ttgctcaaag
    10801 ttataaattg attgctcaac ctcattctgg tgataaacat cgcggtaagt taaccccttt
    10861 gtccagacca gatcaaacac attctcgaca ccctgtaaat acatcgctaa ccgctccaga
    10921 ccataggtga tctctcccgt gataggttta cagttgatgc cgccgacttg ctgaaaataa
    10981 gtaaattgtg ttacttccat tccatttagc caaacctccc accctaggcc ccaagcgccc
    11041 agtgttggat tttcccaatc gtcttcgacg aatcgcacat cattgcaagt tagatcaaaa
    11101 ccaagttcct gcaacgaccc gaggtaaaga tccagaattg tttgaggtgc cggttttaaa
    11161 acaacctgaa attgatagta gtgctgcagg cggtttgggt tatccccata acgaccatct
    11221 ttgggacgcc gtgaaggctg cacataggcg gctcgccagg gctcagggcc aatggcgcgc
    11281 aaaaaagtgg ctgtatggct cgttccagcg ccaacttcca tgtcataagg ttgtagcaat
    11341 gcacagcctt gtcgactcca gtaattttga agcgctaaaa tcatttcttg aaacgtcaat
    11401 ggggtcataa atgcgtaatg aagcaaaata taaacaatgc agtgtaagct acgatgttga
    11461 aaactacata attaaacaat taacaagaaa tcaagcgcta ataattcgga agtcccaatc
    11521 gacttttttc cacgcatcga tttcagcttg caacaaagtt tctgtcagtg gattttgatc
    11581 gagccagacc tgcgaaatga tgagcctgca ctgatgtcct ttaatttcaa gcttgatttg
    11641 tggcaactca atattcatcc ggcttcggta aaataatgta gcaagcctta gcgcgatcga
    11701 ttgagccaaa ttgattgggt ttgttaaaaa ttcactgact ttggtcaatg atccttggtg
    11761 tgaaagaacc aattcactga gctgcgcttg ctccatattg gaaaaccctg gcatgtcagc
    11821 attacccagg atatatgatg aatgtttgtg atagctggaa tgcgcaactg aaatccctac
    11881 ttcatgtaac cgcgctgccc aagaaagcaa ttgaagtgca tcttctactt cctcctcatc
    11941 agaaaaatct gataatagct gtttacccag ttcgcccgcc aatagctcaa ttcggttggc
    12001 ctgcgccgta tcaatgagat aacgctgcat gaattgcttg accgaaacct cacgcatatc
    12061 atcgttatat gagcggccta acagatcata tagaacccct tgccgcaacg caccggtagt
    12121 caaatacatc cgatcgatgc cgagttcagt gaacgcagca gacatgatgg caagccctcc
    12181 agcaatgact ggtgttcgat cactgtggag tccttccaaa ttcactttct tgagatcacc
    12241 cactttcaaa agatattctc gaaactgttc aagccccttt aaagtaattt cttccgtact
    12301 taagctatct ccaaactgat tgagcattaa gatacgtcca agtgctctag ccgttcctga
    12361 cgaaccatag gcttcttgcc agtgacgaga agaaaaatcg gctttaatgc tttgtatttc
    12421 ggtttgagca gccaattcag cacgcttcat attgctttta gtcaacttcc catcgggaaa
    12481 atgatgaagg ctatggctga cacaacccat gtacagactt tctaacttat agggattcag
    12541 ttgatgacca ataatgaatt ccgttgaacc accaccaata tcgataacaa gtcgattatt
    12601 atccgaagct ggtaatgcat gcgccacccc caggtaaatt agccgagctt cttcatgacc
    12661 cgcaatgatt tcaattggaa atcctaaagc gccttctgct tttcttagaa acgcagcagc
    12721 gttcttagca acgcgtagag aattagtgcc taccactcta actgcatggg tgggaaaacc
    12781 gcgcaaccgc tctccaaaac gttttaaaca atccagcgct ctttcttgcg acgattcatc
    12841 gagtttctta tccgctgtga tgccgcctgc cagacggatc atttctttta agctgtctaa
    12901 tgggtaaagt tgtttatcaa caacacgcgc aacttgcaag tggaagctat ttgagcctaa
    12961 atcgacagca gccaaggttg agtattcacg catcgcttta aatttgctga tagctaaaac
    13021 tagctagcat tgatatctat tttgctatcc acgcacttcc cgttcaattt catctgcagt
    13081 aacgtggcgc acatcctttc ctttaaccat atacacaacg tattccgaca tatttttggc
    13141 atgatcccca attcgttcaa tggcttttgc aacaaataaa atttctaagc acgttgaaat
    13201 tttccgcgga tcttccatca taaaggtaat cagttgacgc aaaatagcac gaaactcctc
    13261 atcgacttgc tcatcttgac ggacgatgtg cgctgcagca ttcaaatcca atcttgcgaa
    13321 tgcatccaac gcttgatgaa gcatatcgag tgcaatttca gccacatgct ttatttccac
    13381 aaagcgaggc gtatgtgaac gatctgctgc ataaatgagc ctggccatac gtgcaatttt
    13441 cgctgcctca tctccgattc gctcaagatc ggtaattgtc ttgatgacag tcatgatcat
    13501 acgcaagtca ctcgcggttg gttggcgacg tgcaataatt tgactgcaaa tttcatcaat
    13561 cgagacttcc attgcattga cccgatggtc attcgcaatc acctgatcga ttgtggcttc
    13621 attgcccccg atcaatgctt cgagcgcttg ctcaatttgc tcttcaacga ttccgcccat
    13681 ttgcagcacg cgggttcgaa cttcctcgag atcggcatca aatcttttcg atatatgttc
    13741 tttgttgacc atatgttatc ctttttgagt gatcagaatc accaattgtc ggtcacattg
    13801 ataaccattt catgacaaaa taaagcacag catcaaaccg taatcatctc cacaccatgg
    13861 tcagtaccaa gcaataggat attagccgct tttcgtgcaa ataagccgtt agtcaccaca
    13921 cctgtgattt ggttgagcgt tgtttcaagc tcaactggat taagaatttg caaaccatgc
    13981 acatccaaga taatgttccc attgtccgtc acaaaatctt gcctcaaagc agggtgtccc
    14041 cctaaatatg taatttcacg ggcaacatag ctacgtgcca tcggtatgac ttcgatagga
    14101 agtgggaaag tccctaatac atttactaac ttgctctgat cggtgataca aataaatttt
    14161 ctagcgacgg ctgaaacaat cttttctcgt gtcaaagcac ctccgcctcc cttaatcatg
    14221 tgcaaatgtt tggtaatttc atcggctcca tcgatataaa caggcaaatc cgtaacggta
    14281 ttcaagtcaa gcacttcgat tccatgatct ttgaggcgtt gtgctgttac atttgagcta
    14341 gcaatcgctc cttcaatttt gtgcttaatt tttgctagtt cgtcgatgaa ataatttgcg
    14401 gtagaacctg tacctacacc gacaatcgag cctactggga catattggat tgcggcagcg
    14461 gcagcagccc gtttttgttc atcttgcgtc atgttgttcc tatttcgaga tcaataatta
    14521 atagatagta tattagcgat gagtctatcg atcatgctat agaaatccga actaactcgt
    14581 attggtttaa aatgattaag ttagtcgatt aaactttata tcataactct atgaacgaca
    14641 gccgtgttga ttttgtagta tgaatgtcat taaattgctc ccggatgaat taatcaatca
    14701 aatcgcagcg ggtgaggtca tcgaacggcc cgcttctgtt ctcaaagaag ttcttgaaaa
    14761 ctgtgtggat gctggcgcta cagagatcga tattcatctg atgcaaggcg ggcttaagct
    14821 gattcgtgtc actgacaatg gagtcggcat atcaaaagaa gatcttcctt atgcactgac
    14881 tcgccatgca accagcaaaa tcagtaacca agaagatctg cagaagatcg ttagcttggg
    14941 ttttcgtgga gaagcgctag ctagcattgc ttcagtatcc cgcctgtcgc taaccagttt
    15001 tcaagttggt aatcagcatg cttgggaaat ccaagcagaa ggaagtcaaa caattgcact
    15061 gcagcctgca gcaggatcat taggtacttc gctggaagtt cgcgatttat ttttcaatat
    15121 cccagctcgg cgaaaatttt taaaatccga atcaactgaa ttcgctcatt gtgaagcgat
    15181 ttttcagcgc attgctttat ccaatccaac tatcaccttc aatctatacc ataatggaaa
    15241 attgcgaagt cacttggcaa gtgcagattt tccagaacgt attaaacagt tgctaggcca
    15301 ggaatttagg caaactgcac tttttattac tgaggaagcc gcagatatcc aacttcaagg
    15361 gttgatatcc agaccgactt tctcacggac cacccgtgaa atgcagtatt tttttgtaaa
    15421 tggtcgtttt gtcaaagaca agctattaaa tcatgccatc cgtgaagctt atcgtgatgt
    15481 gcttcatcta gatcgtcatc cagcgtatgt gatatatctt catatcaatc cagataatgt
    15541 tgatgtcaat gtccacccca ctaagattga agtacgtttt cgcgattcac gtgcagtgca
    15601 tcagtttatt tttcacgcta tccataaagc gctggctaat ggtcacaccg agtcgagcga
    15661 agcggatttg cctgactcca aacatcatat tggtgtcgtt acacgcaaca ttaaaaaaat
    15721 acccctagcc tatagtcaaa accggcaagt caatttacca ctgcaatctg ctgcacaacc
    15781 ctccgtattc taccaaacac tatttggtga aaaatcgcat gaaattattg cagcacctga
    15841 tctagccgta caatcagagt ctcacgcatc aaatttctta ggatatgcgt taggacaact
    15901 gttgggtatc tacattttag cccagagtaa tcaaggtttg attattgttg acatgcatgc
    15961 cgcgcatgaa agaattgttt atgaaaaact taaacaagcg cttgatcaac aagcgttacc
    16021 gacacaatct ttactgatac cccatttatt gcaagctgat gcgctcgaca ttacgactgt
    16081 cgaagaaaat gcactgctat taaatcaact cggattcgag atttcgataa cttctcccac
    16141 cacgctagcc atccgtacca tgccttctat cttaaaaaac gctgatattt caaaattaat
    16201 tagcaagcta ttagaggata tccgctcata tggttctagc caattattaa caggtaatcg
    16261 caatgaaata ctagcaacca tggcctgtca tagtgcaatt agagccaatg agctactaac
    16321 tatagaagaa atgaacgtac ttttgcgtga gatggaaaaa actgaacgtt ccgatcaatg
    16381 caatcacgga aggccaacct ggctcgcttt aagcctagaa cagattgata aactattcat
    16441 gcgtggaaat taatagttgt tctataaccc cacccttata ggccaggcta tagaacaatt
    16501 ccccctctta gtaactcgat ttttcctcta ccgatcttgc cttaccgcca ccttcttggg
    16561 catctgctgg aatttgatgg aaaggttgaa tcgtttttcc atactcttca ggattaggcg
    16621 tctctggtgc tgatggttta ccacaagcta ctaatactgc acatgtcaag attgtgaaaa
    16681 agcatttaat tttcataaaa aaaccccttt agctcactcg ctagtaattg atgttgttat
    16741 aagaaactac cactcaatag tagccataaa ttttttgtga aacattgatc ttaatcaaat
    16801 ttcaaagaga aacttggcat tctcaataaa aataaaggag atttacacgg ttgcaaccta
    16861 ggctaactag tcttccctga aaaaggcact cgataattgc caagaagtcg atccggt
//
LOCUS       DAOQKQ010000039        16818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_1558_length_16818_cov_5.431486, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000039 DAOQKQ010000000
VERSION     DAOQKQ010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 16818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16818
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1558_length_16818_cov_5.431486"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /gene="sufD"
                     /locus_tag="PKZ49_04030"
     CDS             <1..1081
                     /gene="sufD"
                     /locus_tag="PKZ49_04030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HND35762.1"
                     /translation="SELARFFLSESESEYRLVFIDGHYVAELSQIDSSEKIIQGNLSS
                     LILSHNQIISSHLGKLAEFQDDLFTALNTAFIQDCACVILPRNVSIARPIHLLFISTQ
                     NETTSYPRCLVVAESGSHATIIEDYVATNENVYVTHSVAEFFLADSAKINHYRIQRDS
                     NQAFHIANHVVSLSASSYYAGVNITFGAQISRFSQRIELGGEGAECGIEGFTLITGQQ
                     LADTHTFIDHIKPQGKSRQIHKCIADGSAHGVFNGAVMVRPHAQQTDSAQMSRSLLLT
                     DKARIDAKPQLEIFADNVKCSHGATVGQLNKEEIFYLRSRGISESVARNLLTYAFGGE
                     VIEHIAVPSLKAQLEETVLLRTQIA"
     gene            1087..2349
                     /locus_tag="PKZ49_04035"
     CDS             1087..2349
                     /locus_tag="PKZ49_04035"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HND35763.1"
                     /translation="MKAADILFQADNVSATCINQFRADFPILNLKVGDKPLVYLDNAA
                     SSQMPQSVIDRLVRYQTTQHANIHRAVHHLSEIATHEYEVARQKLQHFINAREEREII
                     FTSGTTDAINLVMHGYGRKFIQKGNEIILTHLEHHSNIVPWQMLAEEKEAVIRVAPIN
                     DSGELLVEEYEKLFNENTRFVALTHVSNALGTINPIKEMIAFAHQRGVPVLIDGAQAA
                     AHLTIDVQALDCDFYTFSAHKLCGPTGIGILYGKARHLEAMQPFKGGGDMISSVTFEK
                     TTYNTLPYKFEAGTPPIAAAIGFGAAIDYLSNIGLGKIARYEHELLTYATERLSVIPG
                     VKIVGNSVSKVAVISFTMDGIHPHDVGTLLNQEGIAVRTGHHCAQPIMQRFNIPATSR
                     ASFAFYNTQDEVDALIEGIQIVKKVFLS"
     gene            2346..2828
                     /locus_tag="PKZ49_04040"
     CDS             2346..2828
                     /locus_tag="PKZ49_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HND35764.1"
                     /translation="MNSKTIYQEVILDHNRKPRNYGSLDQPSHHAVGHNPLCGDRIDL
                     GLDMINNQINQIAFQGESCAICKASASMMTTAVKGKSPAEAKILIQEFRSMVMGTLNL
                     SEQHHLGRLAVFEGVRDLPTRVKCAILPWHTLHAALHSVSNISTEANEEVAQASTVSI
                     "
     gene            2876..3436
                     /locus_tag="PKZ49_04045"
     CDS             2876..3436
                     /locus_tag="PKZ49_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HND35765.1"
                     /translation="MPKIAEIEGTPNPNALKFVLKEPLTWGVTRSFDSVEQAKDDPLA
                     SALFDIDHVTNVFYVDRWITITQDGEADWQDLARAVADPIRAAPGATSQSAATIAAAD
                     ELLANLSPEDQQRLERINILLDEEVRPFLQNDGGDLHVVGFEGNTLRIHYQGACGTCP
                     SSISGTLRGIENLLRTIEPDIELISA"
     gene            3903..5264
                     /locus_tag="PKZ49_04050"
     CDS             3903..5264
                     /locus_tag="PKZ49_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HND35766.1"
                     /translation="MKLNLKKKNQIISAAIGSMLLIGANTSMVHANTSTNPLSFLDNY
                     GIKFGGWVHGGATFNPYITDGFNGPVTFADQANRVQLNQFNLFLERAVVTEGKKWDVG
                     FRADFMFGTDAIFTQAYGNPAFDVNNGRALDDRGNWDLDICCKSLRTYGIAIPQTYAE
                     IYAPFGNGLNVKVGHFYTPIGYESVPAPNNFFYTHAYTMQYGEPFTHTGVLGNYHINK
                     NFTAMAGAIGGSGTGGWDGNFDRQMGNWGGIGGITWASDDKKTSLNISGTGSSTSTRN
                     SSFWGMFSIVFKHMITDKTHFILQHDHGFADNVLLNNLHFANVNKNAEWYGINMHMYY
                     DISRELSIGVRGEWFRDRDGFRVFSPGRVGAATNHLGNSYALGGATQLASSTPSDYYA
                     VTLGLNWKPARMMNLADTRLKGLNIRPNFRYDVADGLHGSYRPFGGNNDQVVMSLDAI
                     LPF"
     gene            complement(5355..6302)
                     /gene="rluD"
                     /locus_tag="PKZ49_04055"
     CDS             complement(5355..6302)
                     /gene="rluD"
                     /locus_tag="PKZ49_04055"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HND35767.1"
                     /translation="MPSEAKGTSIELIVPQDVAGLRLDQVLAKLLPQWSRSRLQSWIN
                     EKHITVNGQTTTAKQKLWGNETIQIIPPIASHEQADRAEAIPLAIVYEDNDILVIDKP
                     VGLVVHPGNGNKQGTLLNALLYHSSLFANVPRAGIVHRLDKDTSGLLVIAKNIEAQTS
                     LVRQLQQRTVKREYLAVVWGEVKQEGWIDAPIGRHPIHRTRMAITAKGKPARTHYRIL
                     TPFKGYTLLQCNLDTGRTHQIRVHMHSIGHPLIGDSVYTDRTKQLPKEAGYFTHRFHR
                     QALHAYRLTLEHPSLQQPMSWESPIPKDIKELLSQLTQL"
     gene            6361..7161
                     /locus_tag="PKZ49_04060"
     CDS             6361..7161
                     /locus_tag="PKZ49_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HND35768.1"
                     /translation="MGRSLILLLTIWLSACSYFKEKDIDDSQWSPNKFYTEAKNELTN
                     QNYAAAIKLFEGLEARYPYGRYAQQAQLEVAYAHYKDQQQASAIAAADRFIQLYPHHP
                     NLDYAYYIKGLANFHDDLGILGFFTSRIIKQDMSERDSKAARESFDNFKELATRFPKS
                     KYTPDALKRMAHLVNVVARSEIYVARYYMKRKAYVAAVRRAQFILEEYPQTPSTEEAL
                     FIMAKAYDKLGLNDLREDVERVMRRNFPESEYLSDTNPLGRESWWKFW"
     gene            complement(7194..7667)
                     /locus_tag="PKZ49_04065"
     CDS             complement(7194..7667)
                     /locus_tag="PKZ49_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HND35769.1"
                     /translation="MTDYRHILLAVEFSEQDQFVTRKAERIATLFNAKLSIIHVLDNI
                     PMPDTAYGTIIPLDIDSMNELLEGEKNKLLEIGDQLGIQSDSLYLVWGKPEVEIIRIA
                     NQENADLIIVGSHGRHGLALLLGSTASGVLHHAQCDVLAIRLEDGWAGSAMLAPA"
     gene            7970..9544
                     /locus_tag="PKZ49_04070"
     CDS             7970..9544
                     /locus_tag="PKZ49_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M10 family metallopeptidase"
                     /protein_id="HND35770.1"
                     /translation="MPTPSSSSRVNSINKTGDNRIDPLLFGQRWASQSISYSFPSQNS
                     AWSTDSASGYGPRNAGGEPWHPSFLPLSELDRSYFELALQQWANVANVHFNLITESSD
                     SVGDIRVAYSQTAGMADAEAWSYLPTGIAASGDIWINSTSDSAKVDWIPGDYAFLTVL
                     HEIGHSLGLEHPFDDPSFPATLDSMSTTIMSYSALPGDQNSFFDFYPTTPMPLDILAI
                     QYVYGANNQYHQEDTVYRYDDTKTYHETIWDSGGNDWITYAGGKEIAIIDLREGEGSY
                     IGNSVLAFDSAQNPDLAMNIWIAYNVVIENATGGVNDDLLIGNDHANTLIGGEGADDF
                     IGRKGNDVLQGEGGIDTAVYNGQRTQYVVNKTQEGYTLVDSSGGDGSDTMVGVERLEF
                     GDIGLALDLDGNAGLVAKLLGAVFGADAIDQADYVYAGLSLLDTGTTTDALATLALNV
                     AGIQSNDGLVTLLWQNLFGGAPTQNEKSPYVQMLDQGEITQAGLTQAAANTTINQANI
                     DLVGLVQNGIEFNLVA"
     gene            10021..10785
                     /locus_tag="PKZ49_04075"
     CDS             10021..10785
                     /locus_tag="PKZ49_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HND35771.1"
                     /translation="MFKKINKYVLLTVAGLILSNPVAADRTVDDFQVWGNVTALGNFG
                     FINPEKPELKKFRWWMEGQGRFGNDATQFTQSLVRPGVGYAVTDKMVVWVGYAWAPTA
                     EPLTLDTGHPFNEHRIWEQVTWADNFWFGRLSLRSRFEQRFFDQNAPLPGHNDVAHRF
                     RQLVKLAVPLTAINPDLSFIVQSELFIAMTTIDNNAGFISRGFDQNRGFVGLGYKINQ
                     YANVELGYMNQFINRPHSARPDQMMHNLVANLFLNF"
     gene            11315..12007
                     /locus_tag="PKZ49_04080"
     CDS             11315..12007
                     /locus_tag="PKZ49_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35772.1"
                     /translation="MYQSENTFEFNPEMSGFETEQYEGEQYEGEQFEQYEYGEAEWGE
                     VFNESEVMELAAELLEVTNEAELDRFLGNLIKKAGSALGKVVKSPIGQAVGGVLKGVA
                     KKALPIAGGALGAYFGGPLGAKIGSGLASAAGSALGLELETLNQEDREFEGGKQFVRF
                     AANTVKNAVSAPPAANPRSTAQMAANKAAQVYVPNLITSGPALTQPSAMPSKGRGGRW
                     FRRGRNIVIVNC"
     gene            12047..12736
                     /locus_tag="PKZ49_04085"
     CDS             12047..12736
                     /locus_tag="PKZ49_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35773.1"
                     /translation="MHDIDRTQLESNPALENYEQEQYEYGEFGEYGETEWSGEAGIFN
                     ESQAMELAAELLEVSSEEELDRFLGGLIKKAGQVAGKFIKSPVGQQLGGLLKGAAKQA
                     LPMVGSAIGGYFGGGSGAKIGSQVASTAGRIFGLELEGLSHEDQEYEAAKRFVQFAGT
                     AAKNVALSPQAVNPKAVAQSAVAAAARQLAPGLVRNNPVVTSGPCSACGRGTMSGRWV
                     RRGSKIILLGV"
     gene            12743..14032
                     /locus_tag="PKZ49_04090"
     CDS             12743..14032
                     /locus_tag="PKZ49_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35774.1"
                     /translation="MAAASYATWMLEQEARALLTRLKRVKPFALHETMLPAASLLPAA
                     QVGIEQYLMKGRRELRRLIHRYIAWLRGPFGRRAKPAHAQRLFSLLRLRFNAVLTQFD
                     LFNDALTQRSESEIGVWLSGLEIVSADALAIPGHYYEVPPVICYLDRGVGAAIRRTRT
                     RLPGGGENPVAVIRIPRERMVGSGIASSLIHEVGHQSAALLNLVNSLRPVLQGMQRGS
                     GAYSKAWSLWERWVSEIIADFWSIARIGIASTLGLMGVVSLPRAFVFRLNRDDPHPSP
                     WIRVKLSCAIGQALYPHPQWQRLAQLWEAYYPIDGLDTAQQLNYQALQATLPGFVALL
                     VNHRPASLHGRSLAEVLQVQNRQPHRLKALFLAWQQQPAKMYQVSPVLAFAVIGQARA
                     DGRMTPEQESALLGKLLTHWALRSTLAVSATCAMRSV"
     gene            14108..16252
                     /locus_tag="PKZ49_04095"
     CDS             14108..16252
                     /locus_tag="PKZ49_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35775.1"
                     /translation="MNIRSKKIQNCEAPCHGTLRVHLHDSGENKLPIRGADIEVLEWN
                     LGEWRSIETRTTDENGYATFKLLGGDAHRYGLAFKNAFGFSGEPVGCSDDELQEELIS
                     IDCDCTKRISLGAPVNFTQQLAMCGPRSEEQLCQDIVAGQPAKLRISHSAALGNPEIK
                     LSSNQGILSQPSIFEDGMDRVYEIAIDTTGMQGNYSINAAFKHPDGSSIATNLAGTAM
                     RNVQHIDARTTTSLTRTETSFTEDTAFWTGIRNSAEALSFNNYLIFMDWIFCGKQKPR
                     ELSQFESDRYDEKNRIWRGKLLKNRFLPFTDSDAYRVVKAATEAFVMVNCGVHRDDLQ
                     PFDAIKDEAYLNRRDLPIPPYGLNQAFKNDYLVGIDGNGDVLPYLAVIRRKLPDIPIK
                     IKPFEDAANNGTGKIDADECFGLLQEKLTNPCLLELIWSYWHEEGMLVQTTNAITRRF
                     QNVRGSEQDPLSNLEMDPLRPLNNVLWGYIQDEQHRLTVNRRNYEYDHHYGLRLEGKA
                     VQNFRPADTRSKFLEAFHHLLRLCTVFYKQDDDTTVKADAFPVLNALKEVHLILSQGA
                     HNQFGDLPSTARIEMLMQQWLLARPEFRELLPTRIMVAYPEPWMDRVDAMKKLQGWTD
                     TSVLHFRNLGMFGEQLLLSIRWGHWSDEFEPVKALNWARFFRPQAQGYIHAYRAATGV
                     DLSADPMVNTRVDSTLPSVLLQKRLASQQRAM"
     gene            16258..>16818
                     /locus_tag="PKZ49_04100"
     CDS             16258..>16818
                     /locus_tag="PKZ49_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HND35776.1"
                     /translation="MAVINFASALYLGMHHPSQLLQSWQQLTLGVPAALAEPPGAEEV
                     AGKLAQLQGSEQGVLLPSTLHLFWDLFGMLHQQPVALFLDDGAYAIARWGVEQFRAKG
                     GDVYIFRHHDPEALLAQIKLHAQRKKHPVVIADGFCPSCGHAAPINDYLEIVRRFGGL
                     LVLDDTQALGILGASSAWCPPYGEGGG"
BASE COUNT         4654 a         3315 c         3997 g         4852 t
ORIGIN      
        1 tagtgaatta gcgcgttttt ttttgagtga gagtgaatca gaatatcgct tggtatttat
       61 cgatgggcat tacgtggcag agttatctca aattgattct agtgagaaga ttattcaagg
      121 gaatttatct tcattgattc tttcgcataa tcaaattatt tcttctcacc ttggaaagct
      181 agccgagttt caggatgatc ttttcacagc attgaatact gcctttatac aggattgtgc
      241 ttgtgttatt ttgcctcgga acgtatcaat tgctagacca atccatttgt tgtttatttc
      301 aactcaaaat gaaacaacga gttatccgcg ttgtttggtc gtagctgaat caggaagtca
      361 tgcgacgatt attgaggatt atgttgccac gaatgaaaac gtttatgtta cacattcagt
      421 tgcagaattt tttctagctg attctgccaa aatcaatcat tatcggattc aacgtgacag
      481 taatcaggcg ttccacattg ctaatcatgt ggttagtcta tcagcgtcaa gttattacgc
      541 tggggttaat attacatttg gcgctcaaat ttcacgtttt agccagagaa ttgaattggg
      601 gggagagggt gctgagtgtg gcatagaggg ctttacgctg attacagggc agcaacttgc
      661 tgatactcat acttttatcg atcatataaa acctcaaggt aaaagtcggc aaattcataa
      721 atgcatcgct gatggttcag cccatggggt atttaatggg gcagtcatgg ttcgtcccca
      781 tgcgcagcaa accgattctg ctcaaatgag tagaagttta ttgctgacag ataaagcccg
      841 tattgatgct aaaccccagc ttgaaatttt tgctgataat gtcaaatgtt ctcatggcgc
      901 tacagtgggt caattgaata aggaagagat attttattta agaagcagag gcatatcaga
      961 atcagttgca cgcaatttac tgacttatgc ctttggtgga gaagtaattg aacatatcgc
     1021 tgtaccttca cttaaagcac agcttgaaga aactgtactt ttaagaacgc aaatcgctta
     1081 atactaatga aagccgcaga tatcctattt caggctgata atgtttcagc aacgtgtata
     1141 aatcaatttc gcgcagattt tccaattctg aacctgaaag ttggcgataa gccactggtt
     1201 tacttggata atgcggcttc tagtcagatg ccccagtctg ttattgatcg tctcgtacgt
     1261 tatcaaacga cccaacatgc gaacattcat cgggcagttc atcatctttc cgaaattgct
     1321 acccatgaat atgaagttgc acgtcaaaaa ttgcaacatt ttatcaatgc acgcgaagaa
     1381 agagagatca tttttaccag tggaactacc gatgctatta atttagtgat gcatggttat
     1441 gggcggaagt ttattcagaa aggaaatgaa attattctta cccaccttga gcatcactcc
     1501 aatattgtgc cctggcaaat gctagccgaa gaaaaagaag ctgtcattcg agtggcaccc
     1561 attaatgatt ctggggaatt attagtggaa gaatatgaga agctatttaa tgaaaatact
     1621 cgctttgtcg cactgacgca tgtttctaat gcacttggta cgattaatcc gattaaagag
     1681 atgatagctt ttgcccatca gcggggagta cctgtattaa ttgatggtgc ccaggctgca
     1741 gcgcatttaa ccattgatgt tcaggcgctt gattgtgatt tctatacttt ctcagcacat
     1801 aaactttgtg gcccaaccgg aattggtatt ctttatggga aagcgcgaca ccttgaagca
     1861 atgcaaccat ttaagggtgg aggggatatg atttcttccg tgacatttga aaaaacgact
     1921 tacaatactt taccctataa gtttgaagca ggcactccgc cgattgcagc agcgatagga
     1981 tttggcgctg caatcgatta tttatcaaac attggtctag gtaagatagc gcgctatgag
     2041 cacgagcttc taacttatgc aacagagcgg ctgagtgtta ttcccggtgt gaagattgtt
     2101 ggcaattcgg tttcaaaagt tgctgttatt tcttttacca tggatggaat tcatcctcat
     2161 gatgtgggta cgttgctcaa tcaagaaggt attgctgttc gtacagggca ccactgtgct
     2221 caaccaatta tgcaacgatt caatattccg gctacttcgc gtgcttcatt tgctttctac
     2281 aatacccaag atgaggttga tgctttaatc gaagggattc aaattgttaa aaaggttttt
     2341 ttgtcatgaa ttcaaaaaca atttatcaag aagtcatttt ggatcataac cgtaagccac
     2401 gaaattatgg aagcttagat cagccaagtc atcacgcagt tggacacaat cctttatgtg
     2461 gcgatcgaat tgaccttggt cttgacatga tcaacaatca aattaatcaa atagcgttcc
     2521 aaggtgagtc gtgcgcaatt tgtaaagctt ctgcatccat gatgacgacc gcagttaaag
     2581 ggaaatcacc tgcagaagct aaaattctca ttcaagaatt tcggagtatg gtaatgggaa
     2641 ccctgaattt gtctgagcaa catcacctcg gaagattagc tgtgtttgaa ggtgttcgtg
     2701 atctgcctac aagagtaaag tgtgccattt taccttggca taccttgcat gctgcccttc
     2761 actctgtctc caatatctca actgaagcaa atgaagaggt agcgcaagct tcaacggtat
     2821 ctatctagaa ttttagttaa taacacatta ttttttgcag aacattaaaa gcagaatgcc
     2881 taaaattgct gaaattgaag ggacacctaa tcccaatgca cttaaatttg tgctgaaaga
     2941 accgctcacc tggggtgtaa ctcgttcttt tgatagtgtt gaacaagcaa aagacgatcc
     3001 gcttgcttct gcattatttg atattgatca tgtcaccaat gtcttttatg ttgatcgctg
     3061 gataacaatt actcaagatg gggaggctga ctggcaagat ctagcccgtg ccgttgcgga
     3121 tcctattcga gcggccccag gggcaacatc tcaaagtgca gcaacgattg cagcagcaga
     3181 tgagctactt gcaaatctta gcccggaaga tcagcaacgg ctcgaacgta tcaatattct
     3241 attggatgaa gaggtacgtc cctttttgca gaatgatgga ggcgatctcc acgtcgttgg
     3301 ttttgaaggg aatacgctga gaattcatta tcaaggtgcg tgtggaacct gtcctagttc
     3361 aatttcaggt acattaagag gaattgaaaa tttattgaga accattgaac ctgatatcga
     3421 attgatttct gcttaagcga tttggtcaat ttatcctaag tatttggaag caatattttt
     3481 tgttatagtt tagcgcgaag ctttgcttat ccagaattac attctggata agcaaattgc
     3541 cagatcatcc cccgatgctt tcgtttcaga aaaacaacga actcgttgaa tccctttgca
     3601 gccccctaat atgaaatttc tctactattg atcaatgttg gtatacgttc tttattacga
     3661 gtatcccaag gtttctagaa aattaatacc catttcttag agagctatca gtattacttt
     3721 gcaacagtat aatatttttg atcaataagt gttgttagtt tcatacactt gtaaagattt
     3781 tatgaaaatt gttcattgac ctaaatttgt gttgtgtgta aagtcaaacc ctcagacgaa
     3841 gtaaggttat taagttttat attgaaaata ctgaaaaatt ctaaaagaaa gaagagggaa
     3901 gaatgaaact taatctaaaa aagaagaatc agattatttc cgcagcaatc ggtagcatgc
     3961 tactcattgg tgctaatacc agtatggttc atgcaaatac atcaaccaac ccactgagtt
     4021 tcttagacaa ctatgggatt aaatttgggg gatgggttca tggtggcgcg acttttaatc
     4081 cctatattac ggatggtttt aatggtccag ttactttcgc agatcaagcg aaccgggttc
     4141 aattaaatca gtttaatcta ttcttagaac gcgcagttgt tacggaaggt aaaaaatggg
     4201 atgttggctt ccgagcagac ttcatgtttg gtacagatgc gatctttacc caagcctatg
     4261 gtaatcctgc attcgatgtt aataatggta gagcgctgga tgatagagga aactgggatc
     4321 tcgatatctg ttgtaaatca ctacggacct atggcattgc aattccacaa acgtatgctg
     4381 aaatttatgc gccgtttggc aatggtttga atgtcaaagt agggcatttc tatacaccga
     4441 tcgggtacga atctgttcct gctcccaata atttctttta tacccatgct tatacgatgc
     4501 agtatggtga gcccttcact cataccggtg ttttaggtaa ttatcacatc aataaaaatt
     4561 ttacggcaat ggctggtgcg attggtggta gtggaaccgg tggttgggat ggtaattttg
     4621 atcggcaaat ggggaactgg ggtggaattg gtggtattac ttgggcaagc gatgataaaa
     4681 aaacatcact gaatattagt ggtacgggta gttcaacttc aacccgcaac agtagcttct
     4741 ggggtatgtt tagtatcgtg ttcaagcata tgatcacgga caaaacccat tttatcttgc
     4801 aacatgatca tggttttgca gacaacgtct tgttaaataa tctccacttt gcgaatgtga
     4861 ataagaatgc cgagtggtat ggtattaaca tgcatatgta ttatgatatt tctcgtgaat
     4921 tatcgattgg tgtgagaggc gaatggttta gagaccgtga tggtttccgg gtattttcac
     4981 ccggccgtgt cggtgcagca acaaatcacc tcgggaacag ttatgcattg ggtggtgcaa
     5041 ctcagctagc aagtagcaca ccgtctgatt actacgcagt gacactcggt ttaaattgga
     5101 aacctgctcg aatgatgaat cttgcagata cccgcctaaa aggtcttaat attcgtccaa
     5161 atttccgcta tgacgtggct gatggacttc atggatctta tcgacccttt ggcggaaata
     5221 atgatcaagt cgttatgtcg ctggatgcca tattaccttt ctaaattagt aaccaaaatg
     5281 taataaactg aaatgcacct gcgggtgcat ttcttttatg atccttctat ttaaatctca
     5341 cgcaatatag tagatcagag ctgggtaagt tgactgagca attctttgat atcttttgga
     5401 ataggggatt cccagctcat tggttgctgg agggaagggt gctccagggt taagcgataa
     5461 gcgtgcaatg cttgacgatg aaaacgatga gtgaagtacc cagcttcttt gggaagttgt
     5521 ttggtacgat ctgtataaac tgaatcaccg ataagtggat ggcctatgct atgcatgtga
     5581 acccgaattt gatgcgtgcg tcccgtatcc agattgcact gtagcaatgt atatccttta
     5641 aacggggtta atatccgata atgtgtgcga gcaggttttc cctttgcagt aatcgccatt
     5701 cgcgttcgat gaatgggatg tctaccaata ggcgcatcga tccaaccttc ttgcttaacc
     5761 tcgccccata cgacggctaa atactcgcgt ttcacggtgc gttgctgtaa ctgacgaacg
     5821 agactggttt gagcttcaat attttttgca atgaccagca agccgctggt atctttatcg
     5881 agacgatgta cgatacctgc tctcggtaca tttgcaaaca gactggaatg atacaacagg
     5941 gcgttaagca aggttccttg cttattaccg ttacccgggt ggacgactaa accaacaggt
     6001 ttgtcgatga ctagaatatc attatcttca taaacaatgg ctaatgggat tgcttcagct
     6061 cgatcggctt gctcatgaga agcgattgga gggatgatct ggattgtttc attaccccaa
     6121 agtttttgtt tagcagttgt agtttgacca tttaccgtaa tatgtttctc attaatccag
     6181 gactgtaagc gactgcgaga ccattgtggc agcagctttg cgagtacctg gtctaatcga
     6241 agtcctgcaa catcttgtgg gacaattaac tcaattgatg ttcctttagc ttcagagggc
     6301 atggtgagaa cagactttac gttataattt gaagatatta ttgcatcgat aggatctgtc
     6361 atggggcgta gtttaatttt gttactaact atttggctat cagcctgtag ttatttcaaa
     6421 gaaaaggata tagatgatag tcaatggtct cccaataaat tttatacaga agctaagaac
     6481 gaactgacca accaaaatta tgcggctgcg atcaaattat ttgaggggct agaagcacgc
     6541 tatccttatg gacgctatgc acaacaagca cagttagaag ttgcctatgc acattataag
     6601 gatcaacaac aagcctcggc cattgcggcg gctgatcgat tcattcaact ttatccgcac
     6661 catccaaacc tagattacgc ctattacatt aaagggcttg ctaattttca tgatgacttg
     6721 gggattcttg gtttctttac atcccgtatt attaagcaag atatgagcga gcgagattct
     6781 aaggctgccc gagaatcatt tgataatttc aaggaattag ctactcggtt tcctaaaagt
     6841 aagtatacgc cagatgcact caaacgcatg gcccatcttg tcaatgtggt tgcaagaagc
     6901 gaaatttatg tagcgcgata ttacatgaag cgtaaggctt atgtggctgc agtgcggcgc
     6961 gcgcagttta ttttggaaga atacccacaa accccttcga cagaagaagc gctattcatc
     7021 atggctaaag cttatgataa gttaggattg aatgatttaa gggaagacgt tgaacgtgta
     7081 atgcgccgaa atttcccaga aagcgagtac ctttccgaca ctaacccgct tggaagggaa
     7141 tcttggtgga aattttggta gtgcgctcga tttagaattg tttttttctt ttactaagcg
     7201 ggcgccaaca tcgcagaacc tgcccaacca tcctcgagac gaatcgctag cacatcgcat
     7261 tgtgcatgat gaagaacacc actggcggtt gagcctagta ataaagccaa accatgtcgc
     7321 ccatgtgaac caacgatgat gagatcagcg ttttcttggt ttgcaattcg tataatttca
     7381 acctcaggct ttccccaaac taaatacaat gaatcggact gtatacctag ctgatcgcca
     7441 atttctaaca gtttattctt ttcgccttct agtaattcat tcatagaatc gatatcgagt
     7501 ggaatgatgg ttccataagc ggtatccggc atcgggatat tatctagcac atggatgatg
     7561 cttagcttcg cattaaaaag agtggctata cgctcagctt ttcgtgtaac gaattgatct
     7621 tgctctgaga attcaacagc aagcagtata tggcggtaat ctgtcattgt ttatttcctt
     7681 aaagtgtatg aacgcgatca atattttgtt tattttacgc gatctacctt atgttagaaa
     7741 atctgaaata aaattgagtg gtagcgcatg caggaaaata gatggataga tgcgatggaa
     7801 tttaaactgg atgtacaatt tatggtgaga aaaagccact gaatcaaaat taaacggaca
     7861 acgatacctt atttgggtat ttaaccggtt agcatccatc tagaatggta tcaaacgtac
     7921 tacgtgctaa tttgatttta ttgtttatga ttgtttagga aattatctaa tgcccacacc
     7981 gtccagctct agccgtgtaa attcgatcaa taaaacagga gataaccgca ttgacccgct
     8041 attgtttggg cagcgttggg caagtcagtc gatttcctac agctttccat cacaaaactc
     8101 tgcgtggtca accgattcag cctcaggtta tgggccacgc aatgccggtg gtgagccttg
     8161 gcatccatct tttctgccat taagtgaatt agatcgatct tattttgaat tggcgctgca
     8221 gcaatgggca aatgtcgcta atgttcattt taatcttata actgaatcca gcgattcagt
     8281 aggcgatatt cgggttgctt acagtcaaac agcaggcatg gcggatgccg aagcatggtc
     8341 ctatttgcca acagggatag ccgcctctgg agatatttgg attaacagca ccagcgatag
     8401 cgctaaagtg gattggatac cgggtgatta tgcttttttg accgtactcc atgaaattgg
     8461 ccattcgtta ggtttggagc atccttttga tgatcctagt tttccagcga cgctagatag
     8521 catgtctacg acaataatga gttactcagc cttaccagga gatcaaaatt cattctttga
     8581 tttctatccg acaaccccga tgcccctcga tatcttagcc attcaatatg tctatggcgc
     8641 aaataatcaa tatcatcagg aagatacggt ttaccgctac gatgatacta aaacttacca
     8701 tgaaactatt tgggatagcg gtgggaatga ctggattacc tatgcggggg gtaaagaaat
     8761 tgcgattatc gatctccgag aaggtgaagg atcttatatt ggtaacagcg tactagcatt
     8821 cgatagtgct caaaatcctg acttagcgat gaatatctgg attgcctata acgtagtgat
     8881 tgagaatgca accggtggtg ttaacgatga cttattgatt ggtaatgatc atgctaatac
     8941 gttaattggt ggggaaggtg ctgatgattt cattggccgg aaaggtaatg atgtgttaca
     9001 gggggaaggc ggcattgata ctgcggtata caatggtcag cgcactcaat atgtagtcaa
     9061 taaaacacag gaaggttata cactcgttga ttcatcagga ggcgatgggt cagatacgat
     9121 ggtgggagtt gaacgtctag aatttggaga tattggttta gcactggatc ttgatggaaa
     9181 tgcagggctt gtcgctaaat tattgggggc agtatttggt gcagatgcca ttgatcaggc
     9241 tgattatgtg tatgcaggat tgtctttact cgataccgga actactaccg atgcactggc
     9301 cacgttagca ttaaatgttg cgggtattca aagtaacgat gggctagtga ctttattatg
     9361 gcaaaaccta tttggtggtg ctcccacaca gaatgaaaaa tcaccttatg ttcagatgct
     9421 cgatcaaggt gaaattacac aggcaggatt aacccaggct gcggcgaata cgacgattaa
     9481 ccaagccaat atcgatctgg taggtttagt gcaaaatgga attgaattta atttagttgc
     9541 ttgatggtct attaacaggc catagaacat aatatgttca cccaaagggg gcgctccaat
     9601 tggcatcgat aatctgcgaa tttagaaagc gtgaatgaaa aaacgctacg tttttggctc
     9661 gttcgatctg cttcaaaaaa tatttataag tgcgataagt acttaacttc agctatttca
     9721 gtgaaagttt aatgacaata gcactgttta cttcaggggt aattctttac attttcttga
     9781 cagctactgt gttactttct cgacagttac cttgttactg cgtgttcagg gcatgtagct
     9841 aatatattaa gaaaattcct atttactaaa ggaatagggt gtaaatttta taaaggtaag
     9901 agtaactaac tacaatcaac aaacaatcgt taattatgtg agcttatgct gtttaactta
     9961 tataaagaat attagtaaaa atagcttcat aaaatcacat tatttaagca aggagtttac
    10021 atgttcaaaa aaataaataa atatgtttta ctgacagtgg ctggtctgat tctgagtaat
    10081 ccggttgctg cggatagaac cgtagatgat tttcaagtgt ggggaaacgt taccgcgctg
    10141 ggtaattttg gtttcatcaa tccagaaaag cctgaattga aaaaattcag atggtggatg
    10201 gaaggacaag ggcgttttgg taatgacgct actcaattta cgcaatcact ggttcgtccc
    10261 ggggttggtt atgcagttac cgataaaatg gttgtgtggg tcggttatgc atgggcccca
    10321 acggctgagc cattgacatt agatacagga catcccttca atgaacaccg gatttgggag
    10381 caagtaacct gggccgataa tttctggttt ggtcggttgt ctctacgcag ccgttttgag
    10441 caacgattct ttgatcaaaa tgcgccgctt ccaggccata atgatgtcgc tcatcgtttc
    10501 cgccaattgg ttaaactagc agttccattg accgcaatta atccggattt aagctttatt
    10561 gttcaaagtg agttgttcat tgcgatgacc actattgata ataatgctgg ctttatctcc
    10621 cgcggtttcg atcagaaccg ggggtttgtc ggattgggtt ataaaatcaa tcaatatgcc
    10681 aatgttgagt tgggttatat gaaccaattc atcaacagac cgcatagcgc acgcccagac
    10741 caaatgatgc acaacttagt agcaaatctg tttttgaatt tctaggttct tcctacgata
    10801 tggggtctcg catatcgtat aacgttttaa gtagccgatt gattttagct agtatggatc
    10861 aatcggctta ttttttttgc tgatagtcag attgtcactg aataatcgtg taaatatcat
    10921 acagtggctc aatatctctc ttttgattgt gattttgcag tcgaactgaa atctttgtta
    10981 gtaagctggt caattaactt caatttttgc aaggcgaaat gattttcgca ctcgatcgcc
    11041 gatttgtttc ctggaacgaa gcaatcttcg caaagcgcta agcttcattt ttgcccattc
    11101 tattgggtct ctagcattat ttatccttaa ctttcagttg attacctcta ttctttgagt
    11161 ggaattaggt tgagatattt ttttggcacg ctatgtgtat atagctaaag gggaggaagt
    11221 cgaaatcaga ccgatcatcc tgacaggagc gcacaacgct catttatctc aaatctcaag
    11281 cattcgaatt aaaaattagc ggagggccaa taccatgtat cagagtgaaa atacttttga
    11341 attcaatccg gaaatgagtg gttttgaaac cgaacaatat gaaggtgaac aatatgaagg
    11401 cgagcagttt gagcaatatg aatatgggga agcggaatgg ggggaagtat tcaacgaaag
    11461 tgaagtgatg gaacttgcgg ctgagcttct ggaagtaacg aatgaggctg agctcgaccg
    11521 tttcttggga aacctgatta agaaagccgg tagtgcatta ggtaaagtag tgaaatctcc
    11581 tattggacaa gccgtcggag gagtactgaa aggagtagcc aagaaagcat tgccgattgc
    11641 tggtggagca ctgggtgctt attttggtgg gccgctgggt gcaaaaattg ggagtggttt
    11701 ggcctccgca gcaggcagtg cgctgggatt ggaactagaa acacttaatc aggaagatcg
    11761 cgaatttgaa gggggaaaac aatttgtgcg tttcgccgcg aataccgtga agaatgcggt
    11821 atcagcaccg cctgctgcta atccacgcag cactgcacaa atggctgcaa ataaagctgc
    11881 ccaagtttat gttcccaatt taatcacttc agggccagca ttaacccagc catcagccat
    11941 gccaagtaaa gggcgtggag gtcgatggtt ccgccgtggt cgcaatatcg tcattgtcaa
    12001 ctgctaagaa gcaacttcta taaacctatt tgtgaggaga atgatcatgc acgatattga
    12061 tcgaacccaa ctggaaagca accctgcatt ggaaaattat gaacaggagc aatacgaata
    12121 tggagaattc ggtgaatatg gtgaaaccga atggagtggc gaagccggta ttttcaacga
    12181 atctcaagcc atggaattgg cagctgagtt attggaagtt agcagtgaag aagagttgga
    12241 tcgattctta ggcggtctca ttaaaaaagc gggccaagtt gctggaaaat ttattaagtc
    12301 tccagtggga cagcagcttg gtggtttatt gaaaggggca gccaagcaag cactcccgat
    12361 ggtgggaagc gcgattggtg gatatttcgg tggcggtagt ggtgccaaga ttggcagcca
    12421 agtggcttca accgcaggga ggatttttgg cctggaattg gaagggctga gtcatgagga
    12481 tcaggagtat gaagcggcta agcgtttcgt tcagtttgcc ggtactgctg caaaaaacgt
    12541 agccttatca ccgcaggcag tcaatccgaa agcggttgca cagtctgccg tagcagcggc
    12601 tgcgcgccag cttgccccag gtctggttcg caataatcca gttgtaacta gtgggccctg
    12661 ttccgcttgc ggtcggggta cgatgagtgg ccgttgggta cggcgtggaa gcaaaattat
    12721 tttgcttgga gtttaattta ccatggctgc cgcttcttac gctacatgga tgctcgagca
    12781 agaggcccgt gcattactga ctcgcttgaa acgtgtcaag ccttttgctt tgcatgaaac
    12841 catgttaccc gcagcatcat tgttacccgc tgcgcaagtc ggcattgagc agtatctgat
    12901 gaagggacgg cgagaattga gaaggctaat tcatcgctac attgcttggt tacgcgggcc
    12961 atttggccgt agggcaaaac ctgcccacgc acagcgtcta ttttcgttgt tgcggttgcg
    13021 attcaatgcg gtgctgacgc aatttgattt attcaacgat gcgctgacgc agcgtagtga
    13081 gagcgagatt ggtgtttggc tttctggtct tgaaatcgtt tcagcggatg cactggcgat
    13141 tcctggacat tattacgagg tgccgccggt tatctgctat ctggatcggg gtgttggtgc
    13201 agcaattcgc cgcaccagaa cacgattacc aggtggtgga gaaaatccag tagcagtgat
    13261 tcggattccg cgggaacgca tggtggggag cggtattgct tcttccttga ttcatgaagt
    13321 agggcatcaa tctgcagcat tgcttaactt ggtcaattct ttgcggccgg ttttgcaagg
    13381 aatgcaacgc ggtagtggcg catatagtaa ggcttggtca ttgtgggagc ggtgggtttc
    13441 agaaattatc gctgattttt ggtcgattgc tcgcattggc attgcttcaa cgctcgggct
    13501 gatgggtgtc gtaagtttgc cgcgcgcatt tgtattccgc ttgaaccgcg atgatccgca
    13561 tccatccccg tggatacgcg tcaaactaag ttgcgcaatc gggcaagcac tttatccgca
    13621 tccacaatgg caacgcctag cgcagctatg ggaagcctac tatccaatag atgggctgga
    13681 tacagcacaa cagctgaatt atcaggcatt acaagcaaca ttgccgggtt ttgtggcgct
    13741 tctagtcaat caccgccctg caagccttca tgggcgtagc ctagctgagg tcttacaggt
    13801 gcaaaaccga caacctcatc gccttaaggc actgtttcta gcatggcaac agcagccagc
    13861 aaaaatgtac caagtatcac cggttctagc ttttgctgtg atcggtcaag cacgtgccga
    13921 cgggcgtatg acgccagaac aggaaagcgc cctgctggga aaactgctga ctcactgggc
    13981 gctgcgcagc accttggcag tatctgcgac ctgtgcaatg cggtcagtat aacctttatc
    14041 gtcgataaca ggccaatacc aaccaatacc aacgaaatca ttttaacgat ctaatcagga
    14101 ggatgatatg aatataaggt ccaaaaaaat acagaactgt gaggccccat gccatggcac
    14161 gctaagagtt cacttgcatg actcgggtga gaataaacta ccgatcagag gagcggatat
    14221 tgaagttctg gaatggaatc tcggtgaatg gcgatctatt gaaactcgaa ctaccgatga
    14281 aaatggctat gcaaccttta agcttctagg aggtgatgcg catcgttatg gcttagcttt
    14341 taagaatgct ttcggtttta gtggagagcc ggtcggatgt tcagacgatg agctacaaga
    14401 agaattgatt tcgattgatt gtgattgtac gaaacggatt agtcttggag ccccggtaaa
    14461 ttttacgcag caactggcga tgtgtggtcc aagatctgaa gagcaacttt gtcaggatat
    14521 tgtggcaggt caaccagcta aattgcgtat ttcgcatagt gcagctttag gtaatccaga
    14581 aattaaattg tcttctaatc agggcatcct gtctcagcct tcaatttttg aggatggaat
    14641 ggatcgagtt tatgagatag ctatcgatac caccggtatg cagggcaatt attcgattaa
    14701 tgcagctttc aagcatcccg acggctcatc aattgctaca aacttagcag gcacagcgat
    14761 gcggaatgta cagcatatcg atgctagaac gacgaccagt cttacccgaa cagaaacttc
    14821 tttcacagaa gatacggcat tttggacggg tattcgtaat agcgcagaag cactttcatt
    14881 taataattat ctgatcttca tggactggat tttctgtggc aaacagaaac cacgagagct
    14941 aagccaattt gaaagcgatc gctatgatga aaaaaatagg atatggcggg gtaaattatt
    15001 gaagaatcgt ttcttaccgt ttacagactc cgacgcgtat cgtgtcgtca aagcagccac
    15061 tgaggcgttt gtgatggtga attgtggtgt gcatcgtgat gatctacaac cctttgatgc
    15121 tattaaagac gaagcttatt taaatcggcg tgatctaccc ataccaccct atggtttgaa
    15181 tcaggctttt aaaaatgatt atctagtggg aattgatggt aatggcgatg tattacccta
    15241 tctggctgtt atccgccgta agctacccga tatcccgatt aagatcaaac catttgaaga
    15301 tgcggcaaac aacggaacag gaaagattga tgccgacgaa tgttttgggc tgctgcaaga
    15361 aaaacttacc aatccttgct tgcttgaact catatggtcc tattggcacg aagaagggat
    15421 gctggtgcaa accaccaatg ccataacaag gcgattccag aatgtacgtg gctccgaaca
    15481 agatccactt tccaatctcg agatggatcc gctacggcca ctcaacaatg tgctgtgggg
    15541 ttatatccag gatgaacagc atcgcttgac ggttaatcgg cgcaattatg agtacgatca
    15601 tcattatggc ttgcgtctgg aaggtaaagc cgtacagaat ttccgacctg ctgatacgcg
    15661 atcgaaattt cttgaagctt ttcaccatct gttgcggttg tgcaccgtat tttataaaca
    15721 ggatgacgat actacggtta aggccgatgc gtttccggta ctcaatgccc tgaaagaggt
    15781 acatttgatt ctttctcagg gggcgcacaa tcagtttggc gacttgccgt cgacggctcg
    15841 cattgaaatg cttatgcagc aatggttgtt ggccagaccc gagttccggg agttattgcc
    15901 aacacgcatt atggtggcct accctgaacc ctggatggat cgtgtcgatg ccatgaagaa
    15961 attgcaaggc tggaccgata ccagcgtgct gcatttccgt aatctgggta tgtttggcga
    16021 gcagcttttg ctttccattc gttggggaca ttggagcgac gaatttgagc ctgtaaaagc
    16081 gctcaattgg gcgcgctttt tccgaccaca agcacagggt tatattcatg cctatcgtgc
    16141 agcaacaggt gtcgatttgt cagcagaccc aatggtcaat acgcgtgtag attcaacgtt
    16201 gccgtcagta ctactgcaaa aacggttagc aagtcagcaa cgggcgatgt gaggcgcatg
    16261 gcggtgatca actttgcttc agcactatac ctgggaatgc atcaccccag tcaacttttg
    16321 caatcctggc agcagctaac actgggcgtt cctgcagcac ttgccgagcc acctggtgct
    16381 gaggaagtgg ctgggaaatt ggcacagtta caagggagcg aacaaggggt attgctacct
    16441 tccacattgc atctattttg ggacttgttt ggtatgttgc accagcagcc agtggcatta
    16501 tttctggatg atggcgctta tgcgattgct cgctgggggg tagagcagtt tcgtgccaaa
    16561 gggggtgatg tttatatttt ccgacatcac gatcctgaag cattgttggc gcaaatcaag
    16621 ttgcatgcac aaaggaagaa gcaccccgta gtcattgccg atggtttctg tccatcgtgt
    16681 gggcatgcag cgccgattaa tgattatctg gagatcgtaa ggcgttttgg tggtttactg
    16741 gtgttggatg acacgcaagc tttgggaatt ttgggagcgt catcagcatg gtgtcctcct
    16801 tatggagaag gcgggggt
//
LOCUS       DAOQKQ010000040        16670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_1580_length_16670_cov_6.922961, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000040 DAOQKQ010000000
VERSION     DAOQKQ010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 16670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16670
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1580_length_16670_cov_6.922961"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..741)
                     /locus_tag="PKZ49_04105"
     CDS             complement(91..741)
                     /locus_tag="PKZ49_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HND35777.1"
                     /translation="MVRNISEEEELLRKRARRRLLGAITLVTLLVIFLPMLFDEESTQ
                     ERQEIDIQIPSEDLVTENYPWMMPTDPSSDETDASTEQPDELLAMIPQGSPQTAEDAQ
                     EESSNDQGTPIPLSKPQYHKPVTQLNKTPETPQPKAAEGVFVIQLGAFSDPLKAKQQL
                     QNLTASGINGAYVETIKVGHNEMVRVRVGPFSTRKAAEDEYEKLKRMGLSGVVTSR"
     gene            complement(782..2050)
                     /gene="folC"
                     /locus_tag="PKZ49_04110"
     CDS             complement(782..2050)
                     /gene="folC"
                     /locus_tag="PKZ49_04110"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HND35778.1"
                     /translation="MESRSLADWLAYLENLHPFTIDLGLDRVKRVKDNLCLTPSFPVI
                     TVGGTNGKGSICAMLETTLSAAGYRVGCYTSPHLLTFNERIRILQQEVSDTEICRAFE
                     TIESARLQSQVTLTYFEFGTLAAMHLFIESNVDVTILEVGLGGRLDAVNIFDADCAIL
                     ASIDLDHMDYLGETREEIGLEKIGIFRKDRYAICSEPDIPPSVQMKIDELGAKFFRIN
                     QDFSYSTQDNLQWNFYGIHNNVYALPYPSLRGSFQLRNASACLAALDCLKDELPVDNQ
                     AIRRGLVETTLKGRFQVLPGSPRVILDVAHNPAAAKVLAENLDQMGPSFQKTYAVIGM
                     LKDKDIASVIQILKNHIDVWLIGTIHSARGAQAEEIASIISNITHSNAIHSFSDLSDA
                     YAFAQKQAGKNDRICVFGSFYTVSAILAFT"
     gene            complement(2113..3006)
                     /gene="accD"
                     /locus_tag="PKZ49_04115"
     CDS             complement(2113..3006)
                     /gene="accD"
                     /locus_tag="PKZ49_04115"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HND35779.1"
                     /translation="MSWFQRIIPPKIKPKENGEKKVVPEGLWSKCIACEAVLYYTDLA
                     KNFNVCPKCGYHNRISARNRIDMLLDPEERIEIGVEVKPQDPLKFRDSKRYTDRLIQA
                     NSSTDEDDALVVMQGKIKTTPVVVAAFEFGFMGGSMGSVVGERFVRGVRVSINQHLPF
                     VCFAASGGARMQEGLFSLMQMAKTSAALTQMSKEKLPFISVLTDPTMGGVSASFAFIG
                     DIVIAEPGALIGFAGPRVIEQTVRQTLPEGFQRAEFLLEHGAIDLIVDRRHMREKLVN
                     LFTLLMRTPSPKSAATLLPPS"
     gene            complement(3040..3843)
                     /gene="trpA"
                     /locus_tag="PKZ49_04120"
     CDS             complement(3040..3843)
                     /gene="trpA"
                     /locus_tag="PKZ49_04120"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HND35780.1"
                     /translation="MNRIAATFSKLKSANQKVLIPFITAGDPKPEVTVPLMHTLVKAG
                     ANLIELGVPFSDPMADGPTIQRSSERALANGVSLANVIAMVAEFRKTDTLTPVVLMGY
                     ANPVEAMGVDHFISTAKESGVDGILVVDYPPEESTEWAQQLSAAGIDPIFLLSPTTPI
                     ERVKQVAKLAQGYLYYVSLKGVTGASHLDLTEVQTKLAQVRTLVSIPIGVGFGIRDAQ
                     TAKAISKLADAVVIGSRIIEEIEKAPENSLLETLSNLVSSMRSAIDETN"
     gene            complement(3887..5086)
                     /gene="trpB"
                     /locus_tag="PKZ49_04125"
     CDS             complement(3887..5086)
                     /gene="trpB"
                     /locus_tag="PKZ49_04125"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HND35781.1"
                     /translation="MSAYNLPDEKGHFGPYGGKFVAETLMTALEELRTQYLYYQADKA
                     FQTEFAYELKHYVGRPTPIYHAKRWSDHLGGAQILLKREDLNHTGAHKINNTVGQALL
                     AKRMNKKRVIAETGAGQHGVATATVAARQGMECVVYMGSEDVKRQAMNVYRMKLLGAQ
                     VIPVESGSRTLKDALNEAMRDWVTNVESTYYIIGTVAGPHPYPMMVRDFQAIIGKEVK
                     DQMQQEYGRQPDALIACVGGGSNALGLFYPYIDDETVRMIGVEAGGKGINSNQHAATL
                     VTGRPGVLHGNRTYLIQDDHGQIIETHSISAGLDYPGVGPEHAWLKDSNRAEYVAITD
                     DEALSAFHALCRFEGIIPALESSHALAYAAKLSPTLGKDKLLLVNLSGRGDKDMMTVA
                     QYSGITF"
     gene            complement(5073..5699)
                     /locus_tag="PKZ49_04130"
     CDS             complement(5073..5699)
                     /locus_tag="PKZ49_04130"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HND35782.1"
                     /translation="MRTRVKICGITRTEDALAAISSGADAIGFVFWQQSARNILPQQA
                     RPITKTIPAFVTTVGVYVDPTVEWVHETATIANLGLLQFHGNESPEFCDQFSLPYVKA
                     LRIKEDMDLLEYANRYQSAKALLLDTYSANLPGGTGEVFDWTLIPANLPLPIILSGGL
                     TPDNVVHAIAKVKPWAVDVSSGVEASKGIKDINKISAFMQRVKNCECI"
     gene            complement(5746..6555)
                     /gene="truA"
                     /locus_tag="PKZ49_04135"
     CDS             complement(5746..6555)
                     /gene="truA"
                     /locus_tag="PKZ49_04135"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HND35783.1"
                     /translation="MKIALALEYDGSRYHGWQSQPNCLSLQDTLEHALTQIACDDSIR
                     VVAAGRTDAGVHALSQVVHFETNTKRPQSAWVRGVNALLPPDISILWACEVDNRFHAR
                     YSALERCYLYYLLNRPTRPGLFQHKVGWFHEPLNVDDMQKAADSLLGEHDFSAFRTAE
                     CQAKNPVRKLTKLKITQQGDFLKFEFRANGFLHHMVRNILGSLVYVGANRHQPGWITA
                     VLESRDRKRAAPTFSPYGLYLAEVKYDTRWNLPTLNDSPCDITALEVFIKK"
     gene            complement(6613..9009)
                     /locus_tag="PKZ49_04140"
     CDS             complement(6613..9009)
                     /locus_tag="PKZ49_04140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HND35784.1"
                     /translation="MNKIYLRACWLVVLIAFPFLVNAAGLGKLTLGSSLGQPLRAEIE
                     LVSVKNDEIPSLVARVASPEAFRQAGVALAPYHSSLVISVEKRANGQPYIQISSPQSI
                     NEPFLNLLIELNWSSGRLLREYTVLLDPVDTNIAEPASPVVSPVTEAAPQIAGTPTTS
                     AQNNQSKQKPKQPTPKARTSSTAGDTYGPVVKGDTLTRIARQVTPDGVDLNQMLVALY
                     RANRSAFFEKNMNLLKVGTILRIPDQNEVSAIARKEANREVKTQIADWQNYRQRVATA
                     GTTSTNQLPQRQELKQTATGKITATVEEPKLKKEDAPTEVLILSKGEQLGDGKNNVGK
                     PDSQPSEPSQDYVRMMEEDAIAKDRALTEANERVSMLEHNIERLQRLLEIKNAGMADI
                     QKQAEQTQTNPTPPTQLADDKEIASDKPADDIASLQEPAQTEELSLQSDSITAPSLPA
                     PSILQNTPIPAPTPPEPETDFVEQMMGFVSELVDFTTENIEIVGGILITLLTGWLGIS
                     YVRRKKSQANNIDEDEINFDEEKSIRNEEEASTAFSSTFASESAINQFDTKPDTPLSN
                     ELNDVNSPKDSNQTNLSHAASGFFFGKNTDEGFIPSHTNDRDQQQFDSGLENTRATES
                     AQEFKFDMSETNQLSPQLEETIDQKDAASHQDFILDFSSDQSQNTQTASISHDMDFHI
                     DLPEDVNKTSNSFSGTADNEPITQSFDDQLTKLNLTDIKLDLDEDTSQHEKAATHSES
                     VTSEWQEEVATKIDLAKAYLEMDDKEGAKEILEEVLKEGSDEQQMLARTLLDEINTSA
                     "
     gene            complement(9166..10278)
                     /gene="asd"
                     /locus_tag="PKZ49_04145"
     CDS             complement(9166..10278)
                     /gene="asd"
                     /locus_tag="PKZ49_04145"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HND35785.1"
                     /translation="MKQVGFVGWRGMVGSVLMQRMREENDFSLVEPIFFSTSQKNGKG
                     PDIGQEIPPLKDAHDLSELKKMDIIITCQGGDYTNEVFGKLREAGWKGYWIDAASTLR
                     MKDDAVIILDPVNMPVIKQALHEGTKNYIGGNCTVSLMLMGIGGLFENNMVEWMSAMT
                     YQAASGAGAQNMRELLQQMGEAHHVAEELLNNPASSILDIDREVSEKLRDKKFPVENF
                     GVPLAGSLIPWIDREVAQGQSREEWKGQAETNKILGHQSKPVPVDGLCVRIGAMRCHS
                     QALTIKLKQDIPLNEIEDVIAQSNDWVRVIPNEREKTTKELTPVAVTGKLDVHVGRIR
                     KLSMGGEYLTAFTVGDQLLWGAAEPLRRMLRILVEN"
     gene            complement(10411..11472)
                     /gene="leuB"
                     /locus_tag="PKZ49_04150"
     CDS             complement(10411..11472)
                     /gene="leuB"
                     /locus_tag="PKZ49_04150"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HND35786.1"
                     /translation="MKIAVLPGDGIGPEITQQAEKILMALASDGLKIELEHGFIGGSA
                     VDKYNDPFPEATHRLAEEADAILLGAVGGPKYDQLPREKRPEQGLLAIRKALNFFANL
                     RPAQLYPELANASTLKPEIVANLDILIVRELTGDIYFGTPRGIEVRDGQRVGFNTMIY
                     TESEIRRIAHVAFQAARKRSSKLCSVDKMNVLECTQLWRNVVEEVAKEYPDVALSHML
                     VDNAAMQLIRNPKQFDVIVTGNMFGDILSDEASMLTGSIGMLPSASLNEFNKGLYEPI
                     HGSAPDIAGKNLANPLATLLSVSMMLRYTFNQDTAASRIEDAVKKVLASGYRTVDIFE
                     QGMIKVGTAEMGNAVLANL"
     gene            complement(11500..12138)
                     /gene="leuD"
                     /locus_tag="PKZ49_04155"
     CDS             complement(11500..12138)
                     /gene="leuD"
                     /locus_tag="PKZ49_04155"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HND35787.1"
                     /translation="MKKFTDCDGLAVPLDRSNVDTDAIIPKQFLKSIKRSGFGQNLFD
                     EWRFLDTGEPGKENLHRKLNPDFVLNFPRYQGARILVTRDNFGCGSSREHAPWALQDY
                     GFEVIIAPSFADIFFNNCFKIGLLPIVLDSKVVEKLIAQIQATEGYRLAINLEAQTVT
                     TPTKECYHFDVDSFRKHCLINGLDEIGLTLQHTDKIKLFEQKRQSEQPWLFI"
     gene            complement(12147..12272)
                     /locus_tag="PKZ49_04160"
     CDS             complement(12147..12272)
                     /locus_tag="PKZ49_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HND35788.1"
                     /translation="MKLFILLSAFLMTLCLTGCNTLQGFGKDIQRGGEAIERTAQ"
     gene            complement(12310..13719)
                     /gene="leuC"
                     /locus_tag="PKZ49_04165"
     CDS             complement(12310..13719)
                     /gene="leuC"
                     /locus_tag="PKZ49_04165"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HND35789.1"
                     /translation="MKTLYDKLWEAHVVHAESDKPDSTVLLYIDRHLVHEVTSPQAFE
                     GLKLAGRKPWRTRSILAVADHNVPTTNRKNGISDPTSQLQIETLDQNCDELGITEFKM
                     NDLRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFACLAFGIGTSEVEHVLATQCL
                     VAKKSKSMQVLVEGVLGKGVTAKDIALNIIGKIGTAGGTGYAIEFAGNAIRSLSMEGR
                     MTLCNMAIEAGARAGMIAVDDTTIDYIKGRPFAPKGQMWESAVNYWRTLKSDEGAVFD
                     CVVTVNAAEISPQVTWGTSPEMVTSIEGSVPDPESIFDLSKRNDVESALKYMGIKPNT
                     PIQAIYLDKIFIGSCTNSRIEDLREAASILKGKRIADNIKLAIVVPGSGLVKSQAEQE
                     GLDQIFRAAGFEWREPGCSMCLAMNDDKLLPGERCASTSNRNFEGRQGPGGRTHLVSP
                     AMAAAAAVAGHFVDVRLIH"
     gene            13894..15864
                     /gene="acs"
                     /locus_tag="PKZ49_04170"
     CDS             13894..15864
                     /gene="acs"
                     /locus_tag="PKZ49_04170"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HND35790.1"
                     /translation="MAAIESVLHETRIFPPSVEFVQQANISGNESYRALCAEAENDYL
                     GFWSKLAKQHIMWHKPFTKILNDSDAPFFKWFEDGELNISYNCLDRHLRDQPDKVAIR
                     FEADDGKVTHCTYRELYHQVCQFANGLKALNIKKGDRVIIYMPMRIEAVVAMQACARI
                     GAIHSVVFGGFSAKSLHERIVDAGAIAVITADEQTRGGKQHPLKLTVDEAISMGNTES
                     VQSIIVYKHTGTSVPWQNGRDIWWHELIQDKALDCDPEWVNAEHPLFTLYTSGSTGKP
                     KGVQHSSAGYLLGTIVSMQWIFDYKPSDIFWCTADVGWITGHSYVAYGPLAVGATQVI
                     FEGIPTYPDAARFWRIIQNHQVTIFYTAPTAIRSLIKLGVDLPKQYDLSSLRLLGSVG
                     EPINPEAWMWYYTVVGQSRCPIVDTWWQTETGCHMIAPTPGAMPLKPGSCTFPLPGIS
                     VAIVDEAGHDVEQGKGGFLVIKRPFPSQIRTLWGDPARFKKTYFPHDIGDGRYYLAGD
                     SAHRDKDGYFWIMGRIDDVLNVSGHRLGTMEIESALVANPLVAEAAVVGKPHEIKGEA
                     VVAFVVLKEKSPQGEQAQEIAHILRDWVAKEIGPIAKPEEIRFGDNLPKTRSGKIMRR
                     LLRSLAKGEEITQDTSTLENPAILDQLKQSIN"
     gene            15913..>16670
                     /locus_tag="PKZ49_04175"
     CDS             15913..>16670
                     /locus_tag="PKZ49_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HND35791.1"
                     /translation="MNFPNTIDFPHAISAIDAQYHRPGRAAIHLIVEKGVAAIVDTGT
                     KFSIPGVIAVLEHKQISLENVAYVFLTHIHLDHAGGASELMRLLPNTQLVVHPRGAKY
                     MINPAKLIAGVKAVYGEAEFARLYGEIYPIDADRVVEAPDETCIELNGRQLLLLDTPG
                     HARHHYCIFDAQSQSFFTGDTFGVSYRELDVDGLEFVFPTTTPVQFDPDAAHASIDRI
                     MSYHPQFAYLAHYSRIGNLSHHAQQMHALIDEFV"
BASE COUNT         4541 a         3623 c         3471 g         5035 t
ORIGIN      
        1 aaacagaaat aaacccaata cctataacaa tataatcaag aattgtcatc aatcagtagc
       61 aaatactaaa aggtgctatt taggataagt ctatcgactt gttacaactc cgcttagccc
      121 cattcttttg agcttctcat attcatcttc cgctgccttt ctagtcgaaa agggtccgac
      181 gcggactctg accatttcat tatgtccaac tttaattgtt tcaacgtatg cgccattgat
      241 tccacttgca gttaaattct gtagctgctg ctttgcttta agtggatctg aaaaagcgcc
      301 taactggata acaaaaacac cttcagctgc tttaggttgg ggtgtttctg gcgttttgtt
      361 taattgagta actggtttgt gatattgagg cttagataag gggataggtg taccttgatc
      421 gttactagat tcttcctgag cgtcctcagc agtttgaggc gatccttgag gaatcattgc
      481 taataattcg tccggttgct cggttgaagc atcggtttca tcactagaag gatcagttgg
      541 catcatccac ggataatttt ctgtcactaa atcttctgaa ggaatctgga tatctatttc
      601 ctggcgctct tgtgtcgatt cttcatcaaa tagcatcggt agaaatataa ctaataaagt
      661 cactaaggta attgcaccca ataggcgtct acgagcacgt tttcttaaca attcttcttc
      721 ttcgctaata tttctgacca ttattattac cctttgatca ttaatcaaaa atgatacttt
      781 actatgtaaa cgctaaaata gcacttacgg tgtagaatga accaaagacg catattctat
      841 catttttgcc ggcttgcttt tgtgcaaatg cataagcatc cgataaatca gaaaatgaat
      901 gaatagcatt gctgtgggtg atgttgctga ttatcgatgc aatttcttct gcttgggctc
      961 ctcgcgcaga atgaatggtt ccaattagcc atacatcgat atggtttttt agtatttgaa
     1021 taaccgaagc gatatcctta tcttttaaca ttccaataac cgcataggtt ttttggaatg
     1081 atggtcccat ttgatctaaa ttttcagcaa ggacttttgc tgccgctggg ttatgcgcga
     1141 catctaaaat gacccgaggg cttcctggta atacttgaaa tctacctttt aacgttgtct
     1201 ctaccaggcc acgccgaatc gcttgattat caaccgggag ctcatctttg agacaatcca
     1261 atgcggcaag gcaagcagat gcatttctta attgaaatga tcctctaagc gagggataag
     1321 gaagcgcata tacgttattg tggatcccat agaagttcca ttgaagattg tcttgggtgg
     1381 aataactgaa atcctgatta atcctaaaaa attttgcccc tagctcatct atcttcattt
     1441 gtacgctggg tgggatatcc ggttcgctgc aaatcgcata gcgatccttt ctaaaaattc
     1501 caattttttc caggccaatt tcttctctcg tttctccaag ataatccata tgatcaagat
     1561 caatgctggc gagaattgcg caatctgcat caaaaatatt taccgcatct agacgtccac
     1621 ccaagccaac ttctaatatt gtcacatcca cgtttgattc gataaataag tgcatggcag
     1681 ctaatgtgcc aaattcgaaa taagtgagcg tgacttggct ttgcaaccga gcactttcga
     1741 tagtttcaaa agcgcgacat atctcggtat cgctaacttc ttgttgcaat atccggatac
     1801 gctcattgaa agtgagtaag tggggggagg tataacaacc aacccgatag cctgcggcgc
     1861 tgagcgtcgt ttccaacatc gcacagatcg accctttgcc atttgtgcca ccgactgtaa
     1921 ttactggaaa tgaaggcgta aggcaaaggt tatcttttac gcgctttacc cgatccaaac
     1981 ctagatcgat cgtaaaggga tgtagatttt caagataggc gagccaatca gctaatgagc
     2041 gagattccac gaaaaggaga caaccttgaa ggttgagaaa tcaaaaaagt gaaaatactc
     2101 cgcgttgctt atttaagagg gaggtagcaa ggtcgcggct gatttagggc ttggggttcg
     2161 catcaaaagc gtgaataaat taacgagctt ttcacgcatg tggcggcgat ctacaattaa
     2221 atcgatagcg ccatgttcca ataaaaattc agcccgttga aatccctcgg gtaaggtttg
     2281 tcgcaccgtt tgttcaatga cacgtggtcc agcaaaacca atcaatgcac ccggctcagc
     2341 gattacaata tcaccaatga atgcgaaact agccgaaaca cctcccatgg taggatcagt
     2401 taataccgaa ataaacggta gcttttcctt actcatttgt gtcaatgctg cagaggtttt
     2461 agccatttgc atcaatgaga ataaaccttc ctgcatgcgc gctccgccgc ttgcagcaaa
     2521 gcaaacaaac ggtaagtgtt gattaatgct gactcgaaca cctctaacga atcgttcccc
     2581 gacaacagaa cccatggagc cgcccataaa accgaattca aaggcggcaa cgacgaccgg
     2641 cgtggttttt attttacctt gcatcacgac gagcgcatcg tcttcatcgg tgctgctatt
     2701 tgcctgaatt aagcggtcgg tatagcgttt actatctcta aatttaaggg ggtcttgtgg
     2761 ttttacttca acaccgatct caatacgctc ttctggatca agcagcatat cgatccgatt
     2821 tctagcagaa attcgattgt gatagccaca tttggggcaa acgttaaaat ttttagctag
     2881 atcggtataa tataatacag cttcgcaagc gatacatttg ctccataacc cttcgggtac
     2941 aactttcttt tcaccgtttt ctttgggttt tattttgggt ggaataattc gttgaaacca
     3001 gctcattctt tcttgttttt gtaattataa tggtcgacgt taatttgtct catcgatagc
     3061 gctgcgcata gaacttacta gattgcttaa tgtttcaagt aatgaattct caggtgcttt
     3121 ttcgatttct tcaataatac gactgccgat tactaccgca tccgccagtt tcgagattgc
     3181 ttttgctgtt tgtgcgtccc gtataccgaa tccaacccca atgggtatag atactaatgt
     3241 tcggacttgc gctaactttg tttgtacttc ggttagatca agatgggaag cacctgtgac
     3301 ccctttcaag gaaacatagt aaagatagcc ttgcgcaagc ttggcaactt gcttcacacg
     3361 ctcaatcggt gttgtgggag acaataagaa aatgggatca atgccggcag cactaagttg
     3421 ttgtgcccac tcagttgatt cttctggagg gtaatcaacg accagtatcc cgtcaacacc
     3481 cgattctttt gcagttgaaa taaaatggtc aacgcccatt gcttctacgg ggttggcata
     3541 acccattaaa acaaccggag taagtgtatc ggtttttcta aattccgcga ccatagcgat
     3601 cacatttgct aggcttacgc catttgctag cgctcgttca gacgatcgtt gaattgtcgg
     3661 gccatccgcc attggatcag agaaaggaac gcctaactca ataagattag cgcctgcttt
     3721 taccagtgta tgcatcaacg gtacagttac ttcaggtttg ggatcgcctg ctgtaataaa
     3781 tggaatcaag actttctgat ttgcagattt taacttggaa aatgttgcag caattcggtt
     3841 catttagaaa tagtaagtga ttttcaatcg ctacttaagc gttaaattaa aaagtaatgc
     3901 ctgaatattg agcaactgtc atcatatcct tatcaccgcg tccagataag tttactaata
     3961 gtaatttatc ttttccgagt gtaggggaaa gcttggcggc ataagccaaa gcgtggctcg
     4021 attcaagcgc agggattatg ccttcaaagc gacagagtgc atggaatgct gataatgctt
     4081 catcatctgt gatcgcaaca tactcagctc gattactatc tttaagccat gcgtgctcag
     4141 gacctacacc aggataatct aaaccagcag atatcgagtg ggtttcaata atttgaccat
     4201 gatcatcttg aattaaataa gttcggttac cgtgcagcac accgggtcgg cctgttacta
     4261 gcgtggcagc atgttgattc gaattaattc cttttccacc cgcctcaaca ccaatcatac
     4321 gaactgtctc atcatcgata taaggataaa acaaaccaag cgcattggat ccacccccga
     4381 cacatgcaat aagtgcatca ggctggcgtc catactcttg ctgcatttga tcctttactt
     4441 ctttaccaat aattgcctga aaatcccgca ccatcatagg gtagggatga gggccagcta
     4501 ctgttccgat aatataataa gtgctttcca cattggtaac ccagtcgcgc attgcttcat
     4561 tgagggcatc tttgagtgta cgggagccag actcaacggg tatgacttgt gcacctagca
     4621 atttcattct ataaacattc attgcttggc gtttaacatc ttcagaaccc atataaacga
     4681 cacattccat gccttgacgt gctgctactg tcgctgttgc cacaccatgc tgaccggcac
     4741 ctgtttcagc aatgacccgt tttttgttca tacgctttgc tagtagcgct tgaccaactg
     4801 tattattgat cttgtgcgcg cccgtatgat ttagatcttc acgtttgaga agaatctgag
     4861 cgcctcctaa atggtcagac caacgttttg catgataaat aggggtagga cgaccaacgt
     4921 agtgtttgag ctcataagca aactctgttt gaaacgcttt atcagcttga taataaagat
     4981 attgagtccg cagctcctcc agcgctgtca tgagggtttc ggccacaaat ttaccgccat
     5041 aaggaccgaa gtggcctttc tcatcaggaa gattatatgc actcacaatt tttaaccctt
     5101 tgcataaacg cagaaatttt attgatatcc ttaatacctt tgcttgcttc aacaccactt
     5161 gatacatcaa cagcccatgg ttttaccttc gctattgcat gaactacatt atcaggagtt
     5221 aatccgccag acaatataat tggaagcggg agattggctg gaatcaatgt ccaatcaaaa
     5281 acttcacctg ttccacccgg cagattggca ctataagtat ctaacagaag cgcttttgca
     5341 ctttggtatc gatttgcgta ttctagcaga tccatatctt ccttgatacg aagcgcttta
     5401 acataaggga gcgagaattg atcacaaaac tcgggagact catttccatg aaattgtaac
     5461 aaacctaaat tagcgatggt tgctgtttca tggacccact caacggttgg gtcaacgtaa
     5521 acccctactg ttgttacaaa tgcaggtatg gttttagtga tgggtcttgc ttgttgaggc
     5581 aagatgtttc gagcgctttg ctgccaaaag acaaaaccaa tagcatcggc accagaggat
     5641 attgctgcca gtgcatcttc agttcgtgtg ataccacaaa tttttactcg agtacgcatg
     5701 tgagttttat tgttattatg tttaatacgt tgctcagttg ttggtttatt tttttataaa
     5761 tacctctaag gccgtgatgt cgcaaggtga atcatttaat gtagggagat tccagcgagt
     5821 atcatatttt acctcagcta agtataagcc atatggggaa aaagttggtg ctgcacgttt
     5881 ccggtcacga ctttctaata ctgcggtgat ccaaccgggt tgatggcgat ttgcgccgac
     5941 ataaaccaag ctaccaagga tattacggac catgtgatgt aaaaaaccgt ttgccctgaa
     6001 ttcgaatttt aagaaatcgc cttgttgcgt tatcttaagt tttgtcagtt tacggacggg
     6061 atttttggct tggcactcag cggtacgaaa tgcactaaaa tcatgttctc ctagtaagct
     6121 gtcggctgct ttttgcatat catcgacatt cagtggttca tgaaaccaac ctactttgtg
     6181 ttgaaataaa ccaggcctcg ttggacgatt caggagataa tacaaatagc accgctctag
     6241 ggcgctgtaa cgggcatgaa accgattatc aacttcacat gcccacagaa tagaaatatc
     6301 tggcgggagc aatgcgttga cacctctaac ccatgcgctt tgcggacgtt ttgtgttggt
     6361 ttcgaaatga acaacctgtg ataacgcgtg gacgcctgcg tcagttctac ctgctgcaac
     6421 gactctaatc gagtcatcgc aggcaatttg agttagagcg tgctctaatg tgtcttgaag
     6481 tgataaacaa ttaggttgac tttgccatcc atggtagcga ctaccatcat attccagcgc
     6541 taaggcaatt ttcacagaaa ttagctaaag cagccagccc ccacagatcc cttaataaat
     6601 agataaactg tattaagcgc ttgtattgat ctcatcaaga agagttcttg cgagcatttg
     6661 ttgctcatca ctaccttctt ttagcacttc ttctaaaatt tcttttgcac cttccttatc
     6721 atccatttca agataagctt tagctaagtc aattttcgtt gcgacttctt cttgccattc
     6781 cgaggtaact gattctgaat gtgtagcggc tttctcatgc tgtgatgtat cttcatctag
     6841 atctaatttg atatcagtaa ggtttagttt agttagttga tcatcaaatg actgagtaat
     6901 aggttcatta tctgctgttc cagagaaact atttgaagtt ttattcacat cttcgggtag
     6961 atcgatatgg aaatccatat cgtgactgat agaagccgtt tgcgtatttt gggattgatc
     7021 agaggaaaaa tctaaaataa agtcttgatg ggaagccgca tctttttgat caattgtttc
     7081 ttcaagttga ggagacaatt gattcgtttc gctcatatca aatttaaact cttgcgcaga
     7141 ttcagttgca cgcgtatttt ctaatcctga gtcaaattgc tgctggtccc tatcgtttgt
     7201 atggctagga ataaagccct catcggtatt tttgccaaaa aagaaaccgc ttgcagcatg
     7261 ggataaatta gtttggttac tatccttagg agaatttaca tcattaagtt catttgagag
     7321 aggggtatcc ggtttagtat caaattgatt aatagcagat tcggaagcga aagtactgga
     7381 aaacgctgta cttgcttctt cttcgtttct aattgatttt tcttcatcaa aattgatttc
     7441 gtcttcatca atattgtttg cttgcgattt cttgcgccga acatacgaaa ttcctaacca
     7501 ccccgttaat aacgtgataa gtatgccacc cacgatttcg atgttctctg ttgtaaaatc
     7561 aaccagttct gagacaaaac ccatcatttg ttcaacgaaa tctgtttcag gttcaggcgg
     7621 tgtaggtgcc ggtatagggg tattttgcaa tatcgaagga gctggaagac ttggagcagt
     7681 gattgaatct gattgtagtg acagttcttc ggtttgagca ggttcttgca aggacgctat
     7741 atcatcagca ggtttatcag aagcgatttc tttgtcatca gctagctgag tcggtggcgt
     7801 aggattagtc tgcgtttgct ctgcttgttt ttgtatatct gccattccag cattcttgat
     7861 ttcaagcaac cgttgtaaac gctctatatt gtgctcaagc attgagacgc gttcatttgc
     7921 ttcagttaat gctctatctt tggcaattgc gtcctcttcc atcattcgca cataatcttg
     7981 agatggttca gatggctgag aatctggttt acctacatta tttttgccgt caccaagttg
     8041 ttccccctta gataaaatca aaacttcagt aggggcatct tctttcttca acttgggttc
     8101 ttcaaccgtt gctgtaatct tacccgttgc tgtctgcttt aattcttgcc gctgaggtaa
     8161 ttggttcgtt gaagtagttc ctgccgttgc aactctttgt cggtaatttt gccaatcagc
     8221 gatttgagtt ttaacttcac gatttgcttc tttgcgcgcg attgcagaaa cctcattctg
     8281 atcgggaatg cggagtatgg tgccaacctt aagcaagttc atgtttttct cgaagaaagc
     8341 acttcgattg gctcgataca acgcaaccaa catttgattt agatcgactc catctggcgt
     8401 tacttgccgg gcaattcttg ttaaagtatc gcctttaacg acgggaccgt aagtatcgcc
     8461 agctgtacta gatgttctag cttttggggt gggttgtttt ggtttctgct tgctttgatt
     8521 attctgtgct gatgttgtgg gtgtcccagc aatttgcggc gcagcttccg taaccggtga
     8581 aacaacggga ctggcgggtt ctgcgatatt ggtatctaca ggatcaagca ataccgtata
     8641 ttcacgtagt aatcgacccg aagaccaatt taattcgata agaagattta aaaacggctc
     8701 attaatcgat tgcggtgaac taatctgaat atagggttgg ccattcgcac gcttttcaac
     8761 tgaaataact aacgatgaat gataaggtgc aagtgcaaca ccggcctgac gaaaggcttc
     8821 gggtgaagca actcgagcca ccagcgatgg gatttcatcg ttcttgaccg ataccaactc
     8881 gatctctgct cttagcggtt gcccaagtga tgagcctaag gttaacttgc ctaatccagc
     8941 cgcattaact agaaaaggaa atgcaatcaa tactactaac caacacgccc taagatagat
     9001 tttattcact gggttttcct cacaattttt agaacagatt gtatgccaat atcaagccaa
     9061 ttggcaaaac aagtaataat aatcaagtat tagaatgata aatgattctt gactattcta
     9121 tttgacttga ataaagaggc aaaatggctt cgattctaaa agtaattaat tttctaccag
     9181 tattcttaac attcttcgta atggctcagc agccccccag agcaattgat cgcccacagt
     9241 gaaagctgtt aaatactcac cccccatgct caatttacgg atacggccaa catgaacatc
     9301 taattttcct gtaaccgcaa ctggagtaag ctcctttgtt gttttttctc tttcatttgg
     9361 aatcacgcgg acccaatcat ttgattgggc gataacatcc tcaatttcat ttaaaggaat
     9421 atcttgcttt aattttatag ttaatgcctg actatggcat cgcatagcgc caattcggac
     9481 gcaaagacca tcaacaggaa ccggcttgct ttgatgccca agaattttgt tcgtttccgc
     9541 ttgacccttc cattcttctc tactttgtcc ttgcgctact tcacgatcaa tccagggaat
     9601 caggctaccc gctaatggaa caccaaaatt ttcaactgga aattttttat ccctaagttt
     9661 ttccgaaact tctcgatcaa tgtccaatat gcttgaagcg gggttattta ataattcttc
     9721 tgcaacatga tgcgcctctc ccatttgctg taaaagctcg cgcatattct gagcacccgc
     9781 acccgatgcc gcttggtaag tcatggcgct catccattcc accatattat tttcaaatag
     9841 cccgccaata cccatcaaca ttaaactcac cgtgcaatta ccaccgatat agttttttgt
     9901 gccttcatgc aaggcctgtt taatgactgg catattaaca ggatcaagga tgattactgc
     9961 atcatccttc attcttaacg tcgacgctgc atcaatccag tagcctttcc aaccagcctc
    10021 acgtaatttt ccaaagactt cattagtata atcaccacct tggcatgtga tgatgatatc
    10081 catcttcttg agttcagaaa gatcatgggc gtctttgagc ggaggaattt cttggcctat
    10141 atccggacct ttgccattct tctgagaggt agagaaaaaa ataggctcta ccagtgaaaa
    10201 atcattttct tcgcgcatac gctgcatgag aacggatcca accattccac gccaacctac
    10261 aaaaccaacc tgtttcattt aattaaatta acctttgtta aaatagttct tgtattaaat
    10321 atgcacagcc tgtttagatt acaagttgtg attactaata aagttacgat taagcaagaa
    10381 aatgattgta aattcaaata attttcatgg ctatagattt gcgagtactg catttcccat
    10441 ttctgctgta ccaaccttaa tcattccttg ttcaaagata tcgacagtac gataacctga
    10501 tgctagtact tttttaactg catcttcgat gcgtgacgct gccgtatctt gattaaaggt
    10561 ataacgcaac atcatcgaaa cagataacag cgtagctagg ggattagcta aatttttacc
    10621 ggcgatatct ggagcagagc catgaatcgg ttcatataat cctttattga actcgtttaa
    10681 cgaagcggaa ggtaacatgc caattgagcc ggttagcata gatgcttcgt cggatagtat
    10741 atccccaaac atattgcctg ttacaatgac atcaaattgc tttggattgc gaattaactg
    10801 catcgcagcg ttatcgacta gcatatgaga aagagccaca tccgggtatt cttttgcaac
    10861 ttcttctacg acattacgcc ataattgggt acattccaac acattcatct tatcaactga
    10921 acacaacttg ctacttcttt tgcgtgcggc ttgaaatgct acatgggcga tgcggcgtat
    10981 ttcagattct gtatagatca tcgtattaaa accaacccgt tgcccatcgc gtacttcaat
    11041 accacggggt gttccaaagt aaatatcgcc agttagttcg cgaacgataa gaatatctag
    11101 attggctaca atttctggct tcaatgtgga ggcattggcc aattctggat aaagttgcgc
    11161 tgggcgtaga tttgcaaaga aatttaatgc tttgcgaatt gctaatagcc cttgttcagg
    11221 gcgtttttcg cgtggtaatt gatcgtactt aggcccaccg actgccccca gtaagatagc
    11281 atcagcttct tcagcgaggc ggtgcgttgc ttcaggaaaa gggtcgttgt atttatcgac
    11341 cgctgaaccg ccaatgaagc catgttctaa ttcaattttt aaaccatcac tcgcaagcgc
    11401 cattaggatt ttttcagctt gctgcgtaat ttctggaccg atcccatctc cgggtaaaac
    11461 tgctattttc attgcgtgat ttgaattgtg tgaatgcgat taaataaaca accaaggttg
    11521 ttcactttgt cgtttttgct cgaaaagctt aattttatct gtgtgctgta atgtcaagcc
    11581 gatttcatct aatccattga ttaaacaatg tttcctaaat gagtccacat cgaaatgata
    11641 acattctttc gtgggtgtcg tgacagtctg tgcttctagg ttaattgcta agcgataacc
    11701 ttctgtcgct tggatttgag cgataagttt ttcaacaact tttgaatcga gcacgatcgg
    11761 caataatcca attttaaagc agttattaaa aaagatatcc gcaaaactag gtgcaataat
    11821 cacttcaaag ccataatctt gaagcgccca tggtgcatgc tctctgcttg atccacagcc
    11881 gaaattatcg cgcgtcacta agatgcgagc accctgataa cgtgggaaat taagtacaaa
    11941 atcaggattt aattttcgat gaagattttc ttttcctggt tctcctgtat ctaagaatcg
    12001 ccattcgtcg aataaatttt gaccaaaacc cgagcgctta attgatttta agaattgctt
    12061 aggaataatt gcatcggtat ctacgttcga tcgatcaagc ggtacggcca atccatcaca
    12121 atcagtaaat tttttcatgg cttatgttat tgagccgttc tctcgatggc ttcgccgccc
    12181 cgctgaatgt ctttaccaaa accttgtaac gtattgcaac ccgttaggca tagcgtcatc
    12241 aagaatgctg ataaaagtat gaatagtttc atggttgtta tctccttttc atggtttgtc
    12301 agttaaccat tagtgtatca aacgaacatc tacaaaatga ccagcgacag cggcggctgc
    12361 tgccatcgcc ggacttacca aatgggttct tcctccaggc ccttgtctac cttcgaagtt
    12421 acgatttgac gtggaagcac agcgttcccc tggcagaagc ttatcatcat tcatggctaa
    12481 acacattgag cagccaggct cgcgccattc aaatccggca gcgcgaaaga tttgatcaag
    12541 gccttcttgt tcagcctggg atttcactaa accagagcca ggaacaacga ttgccaattt
    12601 aatgttgtcc gcaatacgct taccttttag aattgatgca gcttctcgca aatcttcgat
    12661 acgtgagtta gtgcacgacc caataaaaat tttatcgaga taaatcgctt ggatcggtgt
    12721 attaggtttg atgcccatat atttcagcgc actctctaca tcattacgtt tgctgagatc
    12781 gaaaatcgat tcgggatcag gaacggagcc ttcgatcgac gtaaccattt caggagaagt
    12841 tccccaggta acttgtgggc tgatttcagc ggcattaact gttacgacac aatcaaaaac
    12901 tgcaccctca tcacttttta atgttctcca atagttaacc gcactttccc acatttggcc
    12961 tttgggtgcg aatggtcgac ctttaatata atcgatcgta gtatcatcga cagcaatcat
    13021 tccagcgcgt gcgccagctt cgatagccat gttgcagagt gtcatccttc cttccatgga
    13081 gagtgatcgg atcgcatttc cagcaaattc aattgcgtag ccggttccac cggctgttcc
    13141 aatcttgcca ataatgttta aagcaatatc tttggcagtt acgcctttac ctagcactcc
    13201 ttctactaat acttgcattg atttagactt tttagctacc aagcattgtg tggcgagtac
    13261 atgttccacc tctgaggtac cgatcccaaa agccaagcat gcaaatgcac catgagtgct
    13321 ggtgtgggaa tcaccgcaca ccacggtcat tccgggtagc gttgcacctt gctctgggcc
    13381 aatcacatga acaattcctt gacgtagatc attcatttta aattcggtga ttcctaactc
    13441 atcacaattt tgatctaggg tttcgatttg caattgcgag gtggggtcac tgataccatt
    13501 ttttctattg gtggtaggaa cattgtggtc agcgactgct aaaatggaac gggttctcca
    13561 aggttttctc ccggccaatt tcaatccttc gaatgcctga gggctagtaa cttcatgtac
    13621 taaatggcga tcgatataca aaagcacagt gctatcgggt ttatccgatt ctgcatgtac
    13681 tacgtgagct tcccataatt tgtcgtataa ggtcttcatt tttcaatcgt ctaaaagaac
    13741 aagaatatta tcctatctcg cttaaacaag aaataacttg tcgggattcg atcatcagct
    13801 tttgcgaaat tacgcctact tcattccaag gatgtgaatg ttacaattac tccaatggat
    13861 atttaattaa aacaggatca aaaggattat tttatggctg caatcgaatc cgtgttacac
    13921 gaaacgcgca ttttcccgcc ttcggttgaa tttgtgcagc aagcgaatat ttcggggaat
    13981 gagtcctatc gtgcgttatg tgctgaagca gaaaatgatt atctgggttt ctggagtaag
    14041 ttagccaagc aacatattat gtggcacaag ccttttacca aaatactcaa tgattcagat
    14101 gctccttttt ttaaatggtt tgaggacggt gaattaaata tttcttataa ctgcctggat
    14161 cgccatttaa gagaccaacc tgataaagta gccattcgat ttgaagcgga tgatggaaaa
    14221 gtaactcatt gcacctatcg tgagctttat catcaagtct gtcaatttgc gaatggactt
    14281 aaagcattga atatcaagaa aggtgaccga gtcatcattt atatgccaat gcgcattgag
    14341 gcggtcgttg cgatgcaagc ttgtgcacgg attggtgcga tccactcagt agtgtttggc
    14401 gggttttctg ctaaaagcct tcacgaaaga atcgttgatg cgggagcgat tgcggttatt
    14461 actgccgatg agcaaacgcg aggtggtaag caacacccac taaaattgac cgttgatgaa
    14521 gcgatcagta tgggcaatac ggaatccgtt caatctatca ttgtgtataa acataccggt
    14581 actagcgttc catggcaaaa tggtcgtgat atttggtggc atgagttgat ccaagataag
    14641 gcgctcgatt gtgatccgga atgggtgaat gcagaacatc cattgtttac gctctacaca
    14701 tcaggatcta ccggtaaacc caaaggcgtg caacattcct cagcaggtta tttgcttgga
    14761 accattgtca gcatgcagtg gatttttgat tataagcctt cagatatttt ctggtgtaca
    14821 gccgatgtag ggtggattac cgggcatagc tatgttgcgt atggcccgct cgctgtgggc
    14881 gcaacacagg taatattcga aggtattccc acttaccctg atgcagcgcg tttctggcgt
    14941 attattcaaa atcatcaagt aacaatcttt tatacggccc cgacagcaat tcgatctttg
    15001 atcaagctgg gggtagattt accaaaacaa tatgacttgt cttccctgcg gttgcttggt
    15061 tcagtgggag agcctatcaa tccggaagcg tggatgtggt attacactgt tgtaggtcaa
    15121 tcacgttgcc ccatcgtcga tacttggtgg cagacggaaa caggctgcca catgattgcc
    15181 cctacaccag gcgctatgcc gctcaaacca gggtcatgta catttcctct accgggcatc
    15241 agtgtagcga ttgttgacga agcgggtcat gatgttgagc aaggcaaagg aggattctta
    15301 gtcatcaaac gtccctttcc ttctcaaatt cgaaccctat ggggtgatcc agcgcgtttt
    15361 aagaaaacgt attttcctca tgatattggt gatgggcgct attatttagc gggtgactca
    15421 gcacatcgtg ataaggacgg atatttctgg attatgggga gaattgatga tgtattgaat
    15481 gtatcgggac atcgcttagg gaccatggaa attgaatccg cgctcgtggc taatccatta
    15541 gttgctgaag cagccgttgt tggtaagccg catgagatca aaggagaagc tgttgtagcc
    15601 tttgttgtgc ttaaagaaaa gtcaccgcaa ggtgagcaag ctcaagaaat cgcccatatc
    15661 cttcgtgact gggtagcaaa ggaaatcggc cccattgcaa aaccagaaga aattcggttt
    15721 ggtgataatc ttcctaagac acgatcagga aaaataatgc ggcgattgct tcgttccctt
    15781 gcaaaaggcg aagaaattac gcaagacact tcgacgctag aaaatccagc tattcttgac
    15841 caattaaagc aatcaatcaa ttgaccaatt ttcttgtgaa ttggttacat aaagattttt
    15901 aataataatt ctatgaattt tccaaatact attgattttc ctcatgcgat cagtgcgatt
    15961 gatgcccaat atcaccgacc gggacgcgca gcgattcatt tgatcgttga gaagggtgtc
    16021 gcagcaatcg tcgataccgg aacaaaattt tcaattcctg gtgttatcgc agtactggag
    16081 cacaagcaga tttcactcga aaacgtagcc tatgtttttc taacgcatat ccatttggat
    16141 catgcaggtg gagcaagcga attgatgcga ttgcttccta atacgcaatt agtagtccac
    16201 ccgcgtggag cgaaatatat gataaatcct gccaaattga ttgcgggtgt caaagcagtt
    16261 tacggtgaag cagaatttgc gcgactctat ggagagatat atccgatcga tgcagatcgg
    16321 gtagtggaag cgcctgatga gacttgtatt gagctaaatg gtagacaatt gttgttgcta
    16381 gacactcctg gtcatgcgcg ccatcattat tgcatctttg atgcacagag ccagtcattt
    16441 tttactggtg atacctttgg ggtttcctac cgagaattgg atgttgatgg actagaattt
    16501 gtatttccta ctacgacacc cgttcaattt gatcctgatg ccgctcatgc atccatcgat
    16561 cgcatcatga gttaccatcc tcaatttgct tatttggcac attacagccg aattggcaat
    16621 ttatcgcatc atgcgcaaca aatgcatgcg ctaattgatg aatttgtgca
//
LOCUS       DAOQKQ010000041        16183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_1643_length_16183_cov_6.360181, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000041 DAOQKQ010000000
VERSION     DAOQKQ010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 16183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16183
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1643_length_16183_cov_6.360181"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..1094
                     /locus_tag="PKZ49_04180"
     CDS             162..1094
                     /locus_tag="PKZ49_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HND35792.1"
                     /translation="MSRSDDWYQTPQGEPRGFIESRKLEEVWFHTGTTCNLACPFCLE
                     SSKPGDNRLQQIRFADVEPFIHEALTLGVQQFSFTGGEPFVNKDFVRILATALSYRPC
                     LVLTNATTPLIKRLHQLAPLLKQPHSLHFRVSLDHFDEVQHDAGRGQGMFKLALEGMR
                     HLYGMGFTVSIAHQTIANCDTATVARCFEEVLTAHGLPGNLHRIEFPEFHSPASQVSV
                     PVITPHCMNTYQTEQTRQRFMCAFSRMVVKQAERMRVYACTLVDDDPDYALADTLTDS
                     LAIPVSLKHHRCYSCFRYGASCSEYRSPASVTIP"
     gene            1091..3232
                     /locus_tag="PKZ49_04185"
     CDS             1091..3232
                     /locus_tag="PKZ49_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HND35793.1"
                     /translation="MNKRIWVLLGLLTLIGLYLGFDLGRFLNLETFKTYQHELVEFYQ
                     QQPWWVISVYSISYIVMAALSLPGAGVMSLAGGALFGFWVGVPIVLLSATLGATLAFL
                     AARYLFGNWVEHYLGHRLLTINQALQRDGIFYLFSLRLIPAIPFFIINLLMGLTTLPM
                     LSFFWVSLVGMLAGTAVYVNAGTQLAAIDDPAEIFSPIILSSFLLLALFPWLAKAAIA
                     ISKRHRRYARWKKPARFDRNLIVIGAGAAGLVTAYIAAATRAKVTLIESHKMGGDCLN
                     YGCVPSKSLIRTAHFLNEVRKAQSLGIEPVSVEYQFQDVMARVQRVIQTIAPHDSIER
                     YTQLGVEVIQGKARITSPWTVSVNDQILSTRTIVIATGAHSAIPDLPGLDQVRYYTSE
                     TIWSLQQPPKHLIVLGGGPIGCELAQAFARLAIPVTLVARNRLLMREDNEVAALIESA
                     LQADGVKILIQCKAIRCEVIGGEQRLFLRDQAGIETMLVFDALLCAVGREARVTGYGL
                     EELGINLLPTGTIETNDRLQTIYPNIYACGDVVGPYQFTHIAAHQAWYAAVNGLFDMI
                     KRFRVDYRVIPWSIFTDPEVAHVGLSEQEAQTRKVSYEVTRFDLKELDRAIADEAAYG
                     FIKVLTVPGKDRILGVTIVASHASDLIAEFVLAMKHGLGLNKILSTIHSYPSWSEANK
                     YVAGEWKRQHINPRLLDWIERFHTWRRGGDR"
     gene            complement(3240..6698)
                     /gene="mfd"
                     /locus_tag="PKZ49_04190"
     CDS             complement(3240..6698)
                     /gene="mfd"
                     /locus_tag="PKZ49_04190"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HND35794.1"
                     /translation="MQSKIVLPAPGQFLRYTGLTGSSDTLLLTKLIQSLAPSRAVAII
                     AANALDAQRLFEEIPFFLPEAKIYLLPDWETLPYDTFSPHYDLISERLATLYQVMNAA
                     YDILIVPATTALYRICPKAFLAAHTFFIKQGSTLDLPHFRNQMTLAGYSHVNQVLSPG
                     EYSVRGGLVDLFPMGSALPYRIDLFDDEIETIRTFDADTQRSIYPVSEIRLLPAREFP
                     LDETGRSQFRVNFREKFEGDPSRSKIYKEISKGSTPAGIEYYLPLFFEQTATIFDYLP
                     QDSLICLHQDVYTATDQFWRDTQSRYQLLRGDTDRPLLPPVDLFLTTDNFFGHLKSYA
                     RIQITSQQLEQSDEVLAQSPPSVRVDRHAANPLEKLAGFISAFNSTGGRILLIAESIG
                     RREIIYEYLQDHGLSPGICQSHAEFNSSNSPFMLCAGPIQSGFVLSAELGSDQFAFIT
                     ENELYATHMHGRRNRDSRKTTSTDTLLRDLSEIKIGDPVVHEQHGIGRYLGLVNLDFG
                     EGNSEFLALEYEGNDKLYVPVSHLHLIGRYSGVSPESAPLHKLGSGQWEKAKRRAMQQ
                     VRDTAAELLNLYAQRASRQGHTFKFKQRDYEAFAEGFGFEETTDQAAAIQAVITDLTS
                     GKPMDRLICGDVGFGKTEVALRAAFIAVADGQQVAVLVPTTLLAEQHFQNFSDRFGLI
                     ADQWPVKIAELSRFRSAKEQTLALDLLSKGQIDIIIGTHKLIQEKVKFKNLGLVIIDE
                     EHRFGVRQKEQLKKMRAEVDVLTLTATPIPRTLAMSLEGLRDFSIIATAPQRRLAIKT
                     FVHPFAEGIIREACLRELKRGGQIYFLYNEVSTIQSMFDKLTRLLPEARIHIAHGQMR
                     ESELEHVMRDFYQQRFNLLLCTTIIETGIDIPSANTIIIYRADKFGLAQLHQLRGRVG
                     RSHHQAYAYLLTPDEEALGAQAKKRLEAIQSMEELGSGFYLAMHDLEIRGAGAVLSES
                     QSGEMQEIGFNLYSTMLDSAIKSLKAGQEPDLQHPLGVATEIKLHVPALLPETYCNDI
                     HERLVLYKRMANCEDDGQLDDMQRELIDRFGLLPEPARALLDSHRLRIAAKPLGILRI
                     DASSESILIQFVPNPPIAAERIISLIQSSREYNLSGPDKLKIQTKILDVTERVKRIRK
                     VMDQLV"
     gene            complement(6729..7994)
                     /locus_tag="PKZ49_04195"
     CDS             complement(6729..7994)
                     /locus_tag="PKZ49_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HND35795.1"
                     /translation="MISPFQPGGELEHFLTFATSLAIGLLIGLERERSQAAKAGLRTF
                     ALVSMLGTLAALLSQKTNSPWLLLAGFLLVGTMTIIANLRNHEDPDPGTTTVTAILIC
                     YSLGVLVWYGDTTLAVMLAIVTTILLYFKTELHGLTQNLERRDLISMLQFAVLTFIIL
                     PILPDKNYGPYEAFNPYQIWLMMVLISGISLTGYVALRFIGHRYGAVLLGVLGGLVSS
                     TATTMVFTRRSEENINLAKLAVVVILLANLTVLIRLAVISAALSPKVLPMLLPVLGSG
                     LILGLTVTIFWWRDFTQQEELPIPETKNPTEFSTAAGFGVLYALVLFMSTWLSDIAGS
                     SGLYMVAIISGLTDVDAITLSSLRLLELGKLQISEAVTAITLAIISNIIFKLGLIFII
                     GSSLLNKRTAFGMIMTAIGLAGALFIIVN"
     gene            complement(7987..9735)
                     /gene="recJ"
                     /locus_tag="PKZ49_04200"
     CDS             complement(7987..9735)
                     /gene="recJ"
                     /locus_tag="PKZ49_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HND35796.1"
                     /translation="MPNITTRVFPQAIFDWLVQQGCSPILARIYAARGIEQPSQLETA
                     LSKLLPFEQLKNIAEMAVVLADAIADQKRLLIIADYDSDGATACALAIRALRKFGACV
                     DYLVPNRFEYGYGLTPEIVNLAATSNSEYRPDIIITVDNGISSIEGVEEANRLGIRVL
                     ITDHHLPGEHLPNAAVIVNPNQPSCPFSSKNLAGVGVIFYVMLALRAELRKRGVFNNC
                     FKEPNLASLLDLVALGTVADVVKLDWNNRVLVKHGLHRIRNGKACTGIYALLKVAKRD
                     YQRISTNELGFVIGPRLNAAGRLDDMTLGIECLITDDEGRAFEIASELDRLNRQRREI
                     EATMRDSALDQLALNIKFQQPAESVYSICLYDPNWHQGVIGLIASRVKEKFHRPIIIF
                     APGNEGEIKGSGRSIAGINLRDALDLVSKRNPGLIKKFGGHAAAAGLTIMEHDFQRFQ
                     AAFEQIAQQLLTPADLTQQIETDGDLNETELSLALAQSFAEVVWGHGFPEPTFNARFY
                     VENQRIVGEKHLKLKLRKSISNTSYDAILFFQAEPLPECIQAVFRLQANEFNGNTTLQ
                     LLIEHWSDNNNEIKND"
     gene            complement(9812..10669)
                     /locus_tag="PKZ49_04205"
     CDS             complement(9812..10669)
                     /locus_tag="PKZ49_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HND35797.1"
                     /translation="MNHSSPKISWFEHIGSLLMRQPEDREQLVALLHSAYERNLLDAD
                     ALTMIEGVMHVSEMQVRDIMIPRSHMDTIDISEKPAEFIPFVIEKGHSRFPVTDGSKD
                     QMIGILLAKDLLRYYSEQESFNVREMLRPAVFIPESKRLDILLKDFRSNRNHIAIVVD
                     EYGGVAGLVTIEDVLEQIVGDIEDEYDFDENEANIVAETNGHYRVKAITEVASFNETL
                     GAAFSDQDFDTIGGVVTNKFGRMPKNGESVIIDHYRFTVLRADSRRLHLLKVEKIDAA
                     NPEASDQTD"
     gene            complement(10750..11160)
                     /gene="ybeY"
                     /locus_tag="PKZ49_04210"
     CDS             complement(10750..11160)
                     /gene="ybeY"
                     /locus_tag="PKZ49_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HND35798.1"
                     /translation="MVQYVSKFPETPNRHHFRKWVKAALVQSAEIVIRIVDQEEGLSL
                     NQRFRGKAYPTNVLTFTYDDSSPLAGDIVLCAPVVIREAQEQHKSIDAHYAHLTIHGV
                     LHLQGYDHETDLEAADMEQLESQLMTQLGYPDPY"
     gene            complement(11216..12193)
                     /locus_tag="PKZ49_04215"
     CDS             complement(11216..12193)
                     /locus_tag="PKZ49_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HND35799.1"
                     /translation="MKPKPVEISFSPTDNQRLANLCGALDENLKQIENALDVNIARRG
                     EHFVLKGQSNQTEIAAQVLRNFYNQSHHQLSIEQVQLGLIEAANLSSQLSQSIDPAID
                     ATSSPKLMTRRNNLHARSLRQGQYLQQIQDHDIVFGIGPAGTGKTYLAVASAVDALER
                     EMVSRIILVRPAVEAGERLGFLPGDLVQKVDPYLRPLYDALYDLMGFDKTSRQFERNT
                     IEIAPLAFMRGRTLNQSFIILDEAQNTTPEQMKMFLTRIGFGSKAVITGDITQIDLPK
                     HQKSGLIDAEQVLKQIKGIAFTHFRSEDVVRHPLVQRIVNAYEQHSEQA"
     gene            complement(12278..13603)
                     /gene="miaB"
                     /locus_tag="PKZ49_04220"
     CDS             complement(12278..13603)
                     /gene="miaB"
                     /locus_tag="PKZ49_04220"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HND35800.1"
                     /translation="MNNKLYIKTFGCQMNEYDSDKMADVLHSAKGLELTDKPEEADVI
                     LFNTCSVREKAQEKVFHDLGRVRHLKDKNPNLLIGVGGCVASQEGHEIIKRAPFVDLV
                     FGPQTLHRLPELIRKRELTGRSQVDISFPEIEKFDHLPPARTEGATAFVSIMEGCSKY
                     CSFCVVPYTRGEEVSRPLNDILVEVAHLTLRGVKEVNLLGQNVNAYRGEMDSGEIVDF
                     ALLLEFIHEIPGIERIRFTTSHPKEFTTRLIDVYAKLPKVVNHLHLPVQSGSDRVLAA
                     MKRGYSAIEYKSIIRKIRALRPNISISSDFIIGFPGETEADFEATMKLIEAVYFDESF
                     SFIYSPRPGTPAADLPDDTPQEIKLERLHRLQEKIAQHATLISHNMVGTTQRILIEGY
                     SKKSADELCGRTENNRMVNFIGDPNRIGQFIDIKITAALPHSLRGEILK"
     gene            13743..14198
                     /locus_tag="PKZ49_04225"
     CDS             13743..14198
                     /locus_tag="PKZ49_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HND35801.1"
                     /translation="MPDSINNLTSSSQDKDGLHILRLHDSWIMRWLYLGVGITALLLG
                     ILGIFLPILPTTPFILLAAACFARSSERFHVFLLGNRITGPIIYEWCMHRSIPRRVKR
                     WVYLMITLSFGSSIVIVSAIWLKLMLVILAIVLTLFIWRIPVRDRAEAG"
     gene            complement(14195..14683)
                     /locus_tag="PKZ49_04230"
     CDS             complement(14195..14683)
                     /locus_tag="PKZ49_04230"
                     /inference="COORDINATES: protein motif:HMM:NF023125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3293 domain-containing protein"
                     /protein_id="HND35802.1"
                     /translation="MQTSQFADLYQRQMIILEKSLTQSALSTQLIQNYLSAFYQTDSH
                     FGKMTFRIGQYCDILSRLMIGAKLTNALFITACNPHSQLQCTQINDLLQIQLHKQLTY
                     YSPWIYPGESIDPRNHWPAESSYLTLGIDLLHSIQIGIQFKQNAIVWCNVDAVPRLIL
                     LR"
     gene            14723..15439
                     /locus_tag="PKZ49_04235"
     CDS             14723..15439
                     /locus_tag="PKZ49_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HND35803.1"
                     /translation="MKLFILIILFFFALNVIAEESSRNIVPDTMTERVRACTICHQDG
                     DHDQARSTYYPRIAGKLEGYLFNQLRHFRDGRRYHQPMALLLENMSDDYLQAIARHFS
                     QLNVPYPSPESIDLKSNEIQLVEKLIRLGDPQRNIPACSACHGDTLMGNTPYIPGLLG
                     LPSAYLTAQLSGWRSGSLIRGQVTNCMSEIAKQLSYDEIIAISRWLATQAVEGEAQKV
                     LSPDLIKRCQSIFLEGVAQE"
     gene            15400..>16183
                     /locus_tag="PKZ49_04240"
     CDS             15400..>16183
                     /locus_tag="PKZ49_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HND35804.1"
                     /translation="MSIDFSGRSSARMSPYYFCFTSGFARKCALGYLVFFVLFGVQIN
                     LISAAPGEQDLARGEYLTRVGNCLGCHTAKGGVPFAGGRRLSTAFGTFVTPNITPDKE
                     TGIGHWTEEDFWLAMHEGKSRDGRMLYPAFPYTEFTKVSRADVKSIFQYLQSIAAVSQ
                     VNPAHEIPFPYNSKPLLFGWRKLFFKQGIYEADPTQNEEWNRGAYLVQGLGHCDACHT
                     ARNEVGAKKDAAVGGGQMMGANWYAPSLYSRKEASVTDWSLEA"
BASE COUNT         4296 a         3395 c         3807 g         4685 t
ORIGIN      
        1 tttagcgatc attttgattt ttatggcaca tttgatcggc attttggcat tttcccgggt
       61 tgtggtatgg gagtcccgtt tgatcaagac gatagtcgtc agcctagcag tggctgttgt
      121 tgacgatgga tttatcaatc cgcaatgtcc ataagagaga catgtcgcgt tcagatgatt
      181 ggtatcaaac ccctcaaggt gagccgcgtg ggtttattga atcgcgcaag ctagaggaag
      241 tttggtttca taccggtaca acctgtaatc ttgcttgccc tttttgttta gaaagctcca
      301 aaccaggaga taatcgatta cagcaaatac gatttgcgga tgtagaacct tttatccatg
      361 aagcgcttac gctgggtgtt caacagtttt cattcaccgg gggagaaccg tttgttaata
      421 aggatttcgt gcgcatactt gcaactgcat tgtcgtatcg accttgttta gtgctgacca
      481 atgcaacaac gcccttgatc aaacgattac atcagcttgc gccgctgttg aaacagccac
      541 actcacttca ttttagagtg agtctggatc attttgatga ggtgcaacat gatgcagggc
      601 gcggtcaagg gatgttcaaa cttgcgttag aggggatgcg gcatctctat ggtatgggtt
      661 ttaccgtttc gattgcgcat caaaccattg caaattgcga tacagcgacg gttgcaagat
      721 gctttgaaga agtactgacg gctcacggtc ttccaggtaa tttacaccgt atcgaatttc
      781 ctgaatttca ttccccggca tctcaggtta gtgttcccgt tatcacgccg cattgcatga
      841 atacgtatca aactgagcaa acccgacaac gcttcatgtg cgcttttagc agaatggtgg
      901 ttaaacaagc agagcgcatg cgtgtttatg cttgtacttt agtcgatgat gatcccgatt
      961 atgcattagc agatacctta actgatagtt tagcgattcc cgttagcttg aaacatcatc
     1021 gctgctacag ttgcttccgc tatggtgctt cgtgtagcga gtatcgatca cctgcatctg
     1081 tcacgattcc atgaataagc gcatatgggt attgcttgga ttgctgacgc taattggtct
     1141 ctatttgggt tttgatcttg ggcgttttct gaatttagaa acattcaaaa cctatcaaca
     1201 tgaactagta gagttttatc agcagcaacc ttggtgggtg attagcgttt attccatcag
     1261 ttacatcgtc atggctgctt tgtcattgcc aggtgcaggc gtgatgtcac tcgcgggcgg
     1321 tgccctattt gggttctggg tcggggtccc aatcgtgttg ttgtcagcta cgcttggcgc
     1381 aacccttgcg tttcttgcag cacgctatct ttttggaaat tgggttgaac actacttggg
     1441 tcatcgcttg ctgaccatta atcaagcgct tcagcgtgat ggtatttttt atctgttctc
     1501 gttaaggtta attccagcaa ttcctttttt tataatcaat ttactgatgg gattaacgac
     1561 attaccgatg ctcagtttct tttgggttag tcttgtcggt atgttggcag gtaccgcagt
     1621 ttatgtgaat gcagggacgc agcttgcagc cattgatgat ccggctgaga ttttctcacc
     1681 gattattctt tcttcgtttc tattgttggc actttttccg tggcttgcta aagcggctat
     1741 tgccatcagc aaacggcacc gacgctatgc acgctggaaa aaaccagctc ggtttgatcg
     1801 caatttgatc gtaattggag ctggtgcggc agggcttgta actgcttata ttgcagcagc
     1861 aacacgagct aaagtaacgc tcattgaaag ccacaaaatg ggaggagatt gcctgaacta
     1921 tgggtgcgta ccttccaaat cgctcattcg taccgcacat tttctgaatg aagttagaaa
     1981 ggcgcaatct ctggggattg agcctgtatc tgttgagtat caatttcagg acgtgatggc
     2041 gcgggtacaa cgcgttattc aaacgatagc cccccatgat tcgatcgagc gttatacaca
     2101 gcttggcgtc gaagtcatcc aagggaaagc gcgtattacc tcaccgtgga cggtttccgt
     2161 caatgatcaa attttgtcga cccgtaccat tgtgatcgca acgggtgcac attctgctat
     2221 tcctgatctt ccggggcttg atcaagttcg ctactacaca tcagaaacta tatggtcgct
     2281 gcaacaacct cccaagcatt tgatcgtact agggggaggg cctattggtt gtgagctggc
     2341 tcaagctttt gcacggttgg cgattcctgt tacgcttgtt gcacgtaatc gattgttgat
     2401 gcgtgaagac aatgaagtcg cagcattaat cgaatctgct ttgcaagcag atggtgtgaa
     2461 aattctgata cagtgcaaag cgattcggtg tgaagtgatt ggtggcgagc agcgattgtt
     2521 tcttcgtgat caagcaggta tcgaaaccat gttggtattc gatgcactgc tctgcgctgt
     2581 tggtagagag gcgcgcgtga ctggatatgg gctagaagaa ttgggaatca atcttttacc
     2641 aactggaacg atcgaaacca atgacaggct ccaaacgatt tatcccaata tttacgcgtg
     2701 tggtgatgtc gtgggaccct atcaatttac ccatattgca gcacatcagg cttggtatgc
     2761 tgcggtaaat ggattgtttg atatgatcaa gcgctttaga gtggattacc gtgttattcc
     2821 ttggtctatt ttcacagacc ctgaagtggc ccatgttggg ttatccgagc aagaagcaca
     2881 aacccgtaag gtgagttatg aagtgacgcg atttgatcta aaagaactgg atcgcgcgat
     2941 tgccgatgaa gcggcatatg gttttatcaa ggtgctaaca gttccaggga aagatcgtat
     3001 tttaggggtt acgatagttg ctagccatgc aagcgatcta atcgcggaat ttgtgctggc
     3061 catgaagcat ggacttggac ttaataagat tctcagtacg attcacagtt acccaagctg
     3121 gtcagaagcc aacaaatacg ttgcgggaga atggaagcgc caacatatca atccacgtct
     3181 acttgactgg attgaacgat ttcatacgtg gcgaaggggc ggggatagat gaattgagtt
     3241 cagaccaatt gatccatgac ttttctgatg cgtttgacgc gctctgtgac gtctaaaatt
     3301 ttggtttgaa tttttagttt atcagggcca gaaaggttgt actctcggct actttggatc
     3361 aaagaaataa tccgctccgc tgcaatcggt ggattaggca cgaattgaat gagtatgctt
     3421 tcgctactcg catcgatacg caggataccc agtggtttcg cagcaatccg taaccgatgc
     3481 gaatccaaaa gggctcgcgc tggttcaggc agcaatccaa aacgatcaat gagttcgcgc
     3541 tgcatatcat cgagctgacc atcgtcttcg caattggcca tacgtttata aagcacgagt
     3601 ctttcatgaa tgtcgttgca ataggtttct ggtagcagcg cgggcacatg caatttgatc
     3661 tcagttgcaa ccccgagggg gtgttgtaaa tcgggttctt ggcctgcttt aagcgatttg
     3721 atcgcactat cgagcatcgt actatagaga ttgaagccga tttcctgcat ttcaccgctt
     3781 tgggattcac tcagtacagc gcccgcgccg cgaatttcga ggtcgtgcat ggccagataa
     3841 aaaccagaac ccaattcttc catggattga atggcttcga gtcttttttt agcctgagca
     3901 cctaatgctt cttcatccgg tgttagtaag taggcatagg cctgatggtg ggagcgcccg
     3961 actctacctc gcaattgatg taactgtgcc aagccaaatt tatctgcacg gtaaatgatg
     4021 attgtattcg cgcttggaat atcaataccg gtttcaatga tagtggtaca aagcagtaaa
     4081 ttgaagcgtt gttgataaaa gtcgcgcatg acatgttcca actcgctttc gcgcatttgt
     4141 ccgtgagcga tatggatacg tgcttcaggg aggagacgag ttagcttatc aaacatactt
     4201 tgaatggtgc taacttcatt gtataaaaaa tagatctggc caccgcgctt taattcccgt
     4261 aagcaagctt ctcgaatgat gccttcagca aaggggtgga cgaacgtctt aattgctagc
     4321 cttctttgcg gtgccgttgc gatgatagag aaatcgcgta atccttccaa tgacatggcg
     4381 agtgtgcgcg ggataggggt agccgttagc gtcagaacat ctacttccgc gcgcatcttc
     4441 ttcaattgct ccttctgccg tacaccgaat cggtgttctt catcgatgat gactaaccct
     4501 aggtttttaa actttacttt ttcttggatt aatttatgcg taccgatgat gatatcaatc
     4561 tgtcctttac tcaataaatc tagggcgagt gtctgttctt tggctgaacg aaagcgcgaa
     4621 agctcagcaa tttttaccgg ccattgatca gcaatcaaac caaatcgatc agaaaagttt
     4681 tgaaaatgct gctcggccaa aagggtcgtg ggaacgagta ctgccacttg ctggccgtcg
     4741 gcgactgcaa taaatgccgc acgtagcgct acttcggttt taccaaaccc gacatcacca
     4801 caaatcaaac gatccatagg tttaccggaa gtgaggtcgg tgatgacagc ttgaattgca
     4861 gctgcttgat cggtagtttc ttcaaaacca aagccttccg caaaagcttc gtaatcacgt
     4921 tgtttgaatt tgaatgtgtg accttgacgc gaagcgcgtt gcgcatatag attaagtaat
     4981 tcggctgctg tatctcggac ttgttgcatc gctcggcgtt tggctttttc ccattgtcca
     5041 ctaccaagct tgtgtagcgg tgctgattca ggggaaacgc cactgtagcg accaatcaga
     5101 tgcaaatgcg ataccggaac atagagtttg tcgttgccct catattcaag ggccagaaat
     5161 tcgctgttac cttcaccgaa atcgagattg actaatccca gatagcgacc aataccgtgc
     5221 tgttcgtgta cgacaggatc gccgattttg atctcagaaa gatcgcggag tagggtatct
     5281 gtcgaagtgg ttttgcgaga atctcggtta cgtcgcccat gcatatgggt agcgtaaagc
     5341 tcgttctccg tgatgaaagc aaattggtcg gaaccaagtt ctgcgctcag cacaaaacca
     5401 ctttgtatcg gtcctgcgca gagcatgaat gggctgttgc tgctattgaa ttcagcatgg
     5461 ctttgacaga tcccagggga taagccgtgg tcctgtaaat actcgtaaat gatttcgcgc
     5521 cgaccgatac tctcagcaat taataaaatc ctgccgccag tggaattgaa tgctgagata
     5581 aaaccagcta atttttccag cggattagca gcatggcggt caacccttac gcttggtggt
     5641 gactgtgcca gcacctcatc cgattgctcg agttgctgtg aggtaatttg aatacgtgcg
     5701 taagatttta agtggccgaa aaaattgtct gtcgttaaaa ataggtcgac aggcggcagt
     5761 aaagggcgat cggtatcgcc gcgcaataac tgataacgcg attgagtgtc acgccagaat
     5821 tgatcggtag cggtataaac atcttggtgc aagcagatta aactgtcttg aggtagataa
     5881 tcaaagattg ttgccgtttg ctcaaagaaa agcggtaggt aatattcaat ccctgctggg
     5941 gttgaaccct tactgatttc tttatagatt ttactgcgag aaggatcacc ctcaaatttt
     6001 tcccggaaat tgacacggaa ttgtgaacga cctgtctcat cgagtgggaa ttcacgtgca
     6061 ggtagtaacc gaatttcact aaccggatag atgctacgct gcgtatcggc atcgaaagtt
     6121 cgaatcgttt cgatttcatc atcaaacaga tcaatacgat aaggaagtgc actgcccatt
     6181 ggaaacagat cgactagccc gcctcttacg ctatattccc caggagatag cacttgattc
     6241 acatgggaat agccagccag ggtcatttga ttgcggaaat gcggtagatc gagcgtactg
     6301 ccttgcttga taaaaaaagt gtgagcagct aggaatgctt tagggcagat gcgatatagc
     6361 gcagtagttg cgggtacgat caaaatatcg taggccgcat tcattacttg atagagcgtc
     6421 gctagtcgtt cggaaattaa atcatagtga ggtgaaaagg tatcgtaagg taatgtttcc
     6481 cagtcaggta ataaataaat tttggcttca gggagaaaga atggaatctc ttcaaataat
     6541 cgttgagcat cgagcgcatt agccgcgata atggctactg ctcgggaggg tgctagcgat
     6601 tgaatgagtt tcgtcagcaa taacgtatca ctcgagcccg taaggccagt atagcgcagg
     6661 aattggccag gagccggtaa gactatttta gactgcatgg gaaaatcggt tttttatcga
     6721 caaattgctt aatttactat tataaataag gcgcccgcga gaccaattgc ggtcatgatc
     6781 attccaaaag cagttcgttt gttcaggagt gaactaccaa taatgaaaat caaacccagc
     6841 ttaaaaataa tattcgagat gatcgcgagc gtaatcgcgg tgactgcttc cgatatttga
     6901 agcttaccga gctctaacag ccgaaggcta ctcaacgtga tggcatcgac atcggtaagc
     6961 cctgagataa ttgcgaccat gtataaaccg ctactgcctg caatatcgga gagccaggta
     7021 ctcatgaata acacaagtgc gtaaagtacg ccaaatcctg ctgcagttga aaattcggtt
     7081 ggattcttgg tttctggaat aggcagttct tcttgctgcg taaagtcacg ccaccaaaaa
     7141 atggtaacgg ttagacctaa gataagacca ctaccgagga ctggcagcag catcggtaat
     7201 accttaggag aaagtgctgc actgatcacg gcgagccgga tgagtacggt gagattggca
     7261 agcaagatca caactaccgc aagcttagct aaattaatat tttcttcgct gcgacgtgta
     7321 aaaaccatcg ttgtagccgt gctggaaact aatccaccga gtacgccaag tagcacggcg
     7381 ccatagcgat gaccgataaa acgtaaagca acgtaacctg tgagactaat gcctgaaatc
     7441 aataccatca tcaaccagat ttgataggga ttgaatgctt cataaggccc ataattttta
     7501 tccggtagaa taggcaagat gataaaggtg agaacagcaa attgtagcat cgatatcaag
     7561 tcacgccgtt cgagattctg tgttagtccg tgtagctcag ttttaaaata aagtaatatc
     7621 gttgtcacaa tcgcgagcat gacagcgagg gttgtgtcgc cataccagac cagaacaccc
     7681 aggctatagc agatcaaaat ggctgtaaca gtggttgttc caggatcagg gtcttcgtgg
     7741 tttcttaagt tagcgataat cgtcatggtg ccgacaagca agaatccagc taaaagtaac
     7801 cagggtgaat tggttttttg tgataacaat gctgctagtg tgccgagcat cgagactaat
     7861 gcaaatgtgc gcaatccagc ttttgcagct tgacttcgct cgcgctccag gccgattaag
     7921 agcccgattg cgagactggt tgcaaaggtt agaaaatgct caagttctcc acctggttgg
     7981 aatgggctaa tcatttttta tttcgttatt attatctgac cagtgttcaa ttaatagttg
     8041 tagtgtcgtg tttccattga attcattcgc ttgcaagcga aagacagctt gaatacattc
     8101 cggtaacggt tcagcctgga aaaataaaat cgcatcatag ctggtatttg aaatcgattt
     8161 tcgcagtttt aattttaaat gtttttcacc tacgatacgt tgattttcca cataaaaccg
     8221 cgcattaaag gttggctcag gaaaaccatg accccaaaca acttcggcaa acgattgcgc
     8281 aagcgctaaa cttaattcgg tttcgtttaa atccccatcg gtttcaatct gttgcgttaa
     8341 atcagccgga gtcaacaatt gttgagctat ttgctcaaag gctgcttgaa aacgttgaaa
     8401 atcgtgctcc atgatggtca gtcctgcggc tgcagcgtga ccaccaaact ttttgatcaa
     8461 tcctgggtta cgtttggata cgagatcaag tgcgtccctt aagttgatgc cagcaatcga
     8521 acgtcccgac cctttgattt cgccttcatt ccctggcgca aaaataatga tgggccgatg
     8581 aaatttttct ttgacgcgag aagcaatcaa tccaatgacg ccttgatgcc aattaggatc
     8641 atagagacaa atgctataga cagattcggc gggttgttga aattttatat tgagcgctag
     8701 ctgatccagt gcagaatctc gcatggttgc ttctatttcg cggcgttgcc gattgagcct
     8761 atctaattca cttgcaattt caaacgctcg accttcatca tcggtaatta ggcattctat
     8821 acctaacgtc atatcatcca atcgacctgc tgcattgagt cgtgggccaa tcacaaagcc
     8881 aagctcattc gtcgagatac gttgatagtc gcgttttgcg actttgagca acgcgtagat
     8941 acctgtgcag gctttaccat tgcgaatacg gtgtaggcca tgtttgacca aaactcggtt
     9001 attccagtca agcttaacga catctgctac tgtacctagt gcaacgagat caagtaggct
     9061 agcgaggttc ggttccttaa aacagttatt aaaaacgcct cttttgcgta attctgcgcg
     9121 aagtgccagc atgacgtaaa agatgacccc gacccctgct agatttttgc tggaaaatgg
     9181 gcagctaggt tggttcggat tgacgataac ggctgcattg gggaggtgtt cacctggtaa
     9241 gtgatgatcg gtaatgagta cgcgtattcc taaacggttt gcttcttcaa cgccttcaat
     9301 gctgcttatc ccgttgtcga cggtaatgat gatatcaggt cgatattcgg aattactggt
     9361 tgcagcaagg ttgacgattt caggggttag gccgtagcca tactcaaagc gattgggtac
     9421 tagatagtct acacaggctc caaattttct aagtgcccga atagctaatg cacaggcagt
     9481 tgccccatcc gaatcataat cagcaataat gagtaagcgt ttttggtcgg cgatggcgtc
     9541 tgctaggact actgccatct cagcaatatt ttttagctgc tcaaatggca ataattttga
     9601 taacgccgtc tctaattgac tcggttgttc aattccacgt gctgcgtaga tacgcgctag
     9661 aatcggggag caaccttgct gaactagcca atcaaaaata gcttgaggga aaacacgggt
     9721 agtaatatta ggcatttgcc taatagttta gcatggagta aaacaggatt tagcgggagc
     9781 ttcagaccca tgaacacggg cgtgaatcat tttaatcagt ctgatctgat gcctcagggt
     9841 tagcagcgtc gatcttttcg accttgagta aatgcaggcg ccgactatca gcgcggagta
     9901 ccgtgaatcg ataatgatcg ataatcacag actcaccatt cttaggcatt ctaccgaatt
     9961 tattagtcac gacacctcca atcgtatcaa aatcttgatc actgaatgct gcgccgagtg
    10021 tttcattaaa actagcaacc tcagttatgg ctttgacacg ataatgcccg ttcgtctctg
    10081 caacaatatt tgcttcgttc tcatcgaaat catattcgtc ttcaatatca ccgacaattt
    10141 gctctagcac atcttcgatg gttaccaggc cagcgacacc accatactca tccacaacaa
    10201 ttgcgatatg gttgcgattg ctacgaaagt cttttagcaa gatgtcgagt cgcttggatt
    10261 cagggataaa cacggcggga cgcagcattt cacgaacatt aaaactctct tgctcagaat
    10321 agtaccgcag caaatcctta gcgagcagta ttcctatcat ttgatcttta cttccatcgg
    10381 ttactgggaa acgagaatgc cccttctcga tgacaaaagg gatgaattca gcaggtttct
    10441 cactgatatc gatggtatcc atatgggagc gcggaatcat gatgtcgcga acttgcattt
    10501 ccgacacatg cattacacct tcaatcatgg ttagtgcatc cgcatcaagc aaattccttt
    10561 cgtacgcaga atgtaagagc gctactagct gttcgcgatc ttctggttgt cgcatgagga
    10621 gtgaaccgat atgctcaaac caactgatct tgggagagga atgattcatg attagtctca
    10681 tcatttataa gttatgtgtt atttggatgt agctaaaaca agttattaat atctgcaacg
    10741 cttatctaac taataggggt ctggatagcc cagttgagtc ataagctgtg attcgagctg
    10801 ctccatatct gccgcctcta aatcagtttc atggtcataa ccctgaaggt gcaatacacc
    10861 gtgaatggtg aggtgtgcgt aatgggcgtc aatacttttg tgttgttctt gcgcttcacg
    10921 aattactacc ggcgcgcata gtacaatatc gccggctaaa ggcgatgagt catcataggt
    10981 aaatgtcaaa acattggtag gataagcttt tcccctaaag cgctgattta gtgataggcc
    11041 ctcttcttga tcaacgatgc gaattacgat ttctgcgctt tgtactaatg ctgctttgac
    11101 ccatttacga aaatggtggc gattcggtgt ttcggggaac tttgaaacat attgaaccat
    11161 cagcgataat ttattcgagg ttttctcaac agtattgctc ggaatagaga taggtttatg
    11221 cttgttcgct gtgctgctca taagcattaa caattctttg tacgaggggg tgacgcacca
    11281 cgtcttctga tctgaaatga gtaaaagcaa tacctttaat ttgctttaac acctgctctg
    11341 catcaattaa tccacttttc tgatgtttgg gtagatcaat ttgagtgata tcaccggtaa
    11401 tcactgcttt agatccaaag ccgatgcgtg tcagaaacat cttcatttgc tcgggtgtcg
    11461 tattttgtgc ctcatctagg atgatgaaag attgattcag cgtgcggccg cgcataaatg
    11521 ccagtggtgc aatttcaatt gtgttgcgct cgaattgacg actcgtttta tcaaatccca
    11581 ttaaatcata cagtgcatca taaaggggac gtaggtaagg atctactttc tgaactagat
    11641 cgcctggtaa aaaaccaagt cgttctcctg cttcaacagc cggtctcact agaatgatcc
    11701 gcgataccat ttctcgttcg agtgcatcga ctgcgcttgc aactgcaaga taagttttac
    11761 ctgtacctgc ggggcctatg ccaaaaacga tatcatgatc ttgaatctgt tggaggtatt
    11821 ggccttgacg taaacttcgc gcatgaagat tatttcgacg tgtcatcaac tttggtgatg
    11881 aggtggcgtc aattgcagga tcaatcgact ggctcagttg actcgataaa ttagcggctt
    11941 cgatcaagcc taattggact tgctcaatac ttaactgatg atgggattga ttataaaaat
    12001 tcctaagaac ttgcgctgcg atctctgttt gattggattg ccccttgaga acaaaatgct
    12061 cacctcgtct ggcaatatta acatctaggg cattttcgat ttgtttgaga ttttcatcta
    12121 atgccccgca gaggttagca aggcgctgat tatccgtggg ggagaaagaa atttctacag
    12181 gtttaggttt caaaacgata attaattgaa aacgattagc gcattctcgc aggggttaaa
    12241 tgagtttgca agtgttaaaa gccattctct taatcgttta tttcagaatt tctccacgca
    12301 gggaatgagg caacgccgct gtaattttaa tatcgatgaa ctgaccgatt cgattcggat
    12361 caccaataaa gttaaccatg cgattatttt ctgttctacc gcataattcg tctgcacttt
    12421 tcttagaata gccctcaata aggatacgtt gagttgtgcc aaccatatta tgactgatta
    12481 aagttgcgtg ttgcgcaatt ttttcttgta aacggtgcag tcgctctagt ttgatttctt
    12541 gaggtgtatc atccggtaag tcagcagcag gggttcctgg tcgtgggcta taaatgaagc
    12601 taaacgattc atcgaaataa actgcttcga tcaatttcat ggttgcttca aagtcagcct
    12661 ctgtttcacc tggaaagcca ataataaaat cagaactaat cgagatatta ggacgtaaag
    12721 cccttatttt acggataatg gatttgtact cgatcgcgct atagcctcgt ttcattgcag
    12781 cgagaactcg atcagaacct gattgaacgg gtaagtggag gtgattgact actttgggta
    12841 gctttgcata aacatcgatg agtcgagttg taaattcctt ggggtgcgag gtggtgaagc
    12901 ggatacgttc tatacctgga atttcatgaa tgaattcaag taataatgca aaatcgacaa
    12961 tttcgccgct gtccatttcg ccgcgatagg cgtttacatt ttggccgagt aagttgactt
    13021 ctttaacgcc tcgcaacgtt aaatgggcaa cctcaaccag aatgtcattc agtgggcgtg
    13081 atacttcttc accacgggta tagggaacaa cacagaagct gcaatattta ctgcaacctt
    13141 ccatgataga aacaaaggcc gtagcccctt cggtgcgcgc aggaggcaaa tggtcaaatt
    13201 tctcgatttc tggaaaagaa atatcaactt gagaacgacc cgttaattca cgctttctga
    13261 ttaattcagg taaacgatgt agggtttgag gaccaaacac gagatcaaca aaaggggcac
    13321 gcttaattat ctcatgccct tcttggctag caacgcatcc ccctacgccg attagcaagt
    13381 ttggattctt atcttttaaa tgccgaaccc tcccaagatc atggaaaacc ttttcttgcg
    13441 ctttttcacg aactgagcat gtattgaaca agataacatc ggcttcttca ggtttatcgg
    13501 ttaactcaag acctttagcg gaatgaagga catcagccat tttatccgag tcatactcgt
    13561 tcatttggca accaaatgtt ttaatataaa gtttattatt cactgattaa aaaaattaaa
    13621 ctgcagaaag cagaacaatt atttagtata tatgggttgt gctcaggatt ctatgcttat
    13681 ccttaggcaa gtacttacta cagggttact ttaattcctt ctagccgcaa ccccaaatta
    13741 tcatgcctga ttcgataaat aatttaactt ccagttcaca agacaaagat ggtctgcata
    13801 ttctgcgtct acatgattca tggattatgc gctggcttta cctgggagtg ggtatcacag
    13861 ccttattgct gggtatttta gggatatttc tgcctatttt gccgactacc ccttttattt
    13921 tattagcagc cgcatgtttt gcaaggagct ccgaaagatt ccatgtgttt ttactcggta
    13981 atcgtatcac tggaccgatt atctacgagt ggtgcatgca tcgcagcatt ccacgtcgcg
    14041 tcaagcgttg ggtttatttg atgattactt tgtcttttgg aagctcgatc gttattgtgt
    14101 cagcgatatg gctcaaacta atgctagtaa ttctagctat cgtgctgact ctatttattt
    14161 ggcgcattcc tgtaagggat agagccgaag ctggttagcg taacagaatt aaccgaggaa
    14221 ctgcatcaac attacaccaa acgattgcgt tttgtttgaa ttgtatcccg atttgaatcg
    14281 aatgaagtaa gtcgattcca agcgtaagat aactggattc tgctggccag tgatttcgtg
    14341 gatcaatact ctcgcctggg taaatccagg gtgaataata ggttaattgc ttatgaagtt
    14401 ggatttgtaa tagatcattg atttgagtgc attgaagttg gctatgagga ttgcaagcag
    14461 tgataaatag cgcattggtt agctttgctc caatcatcaa gcgtgaaagt atgtcacaat
    14521 attgaccaat tctgaacgtc atttttccaa agtggctatc tgtttgatag aaggcactaa
    14581 gatagttttg gattagttgg gttgagagcg cgctttgcgt taagcttttt tctagaatga
    14641 tcatttgacg ctgataaaga tcagcaaatt gggatgtctg catatcgaag gaaaggtgtt
    14701 attaggatat gagtgagcac gtatgaagct gtttatttta atcattttat ttttctttgc
    14761 gctaaacgtg attgcagagg agtcatcacg taatattgtt cctgatacca tgactgagcg
    14821 cgttagggca tgcacgattt gtcaccagga tggagaccat gatcaagcga ggagtactta
    14881 ttatcctcgc atcgctggaa aacttgaagg gtatctattt aaccagttac gccactttcg
    14941 agatgggcgg cgatatcacc aacctatggc gctcttactt gagaatatgt ctgatgacta
    15001 tttgcaagcg atagcacgtc atttttctca attgaatgtt ccgtatcctt cacctgaatc
    15061 gattgattta aaatctaacg aaattcaatt agtagaaaaa ctcattcggt tgggtgatcc
    15121 acaacgcaat ataccagcat gtagtgcttg tcatggtgat accttaatgg gtaatacacc
    15181 gtatattcca ggtttattgg gcttacctag cgcttatttg acagcgcaat tgagtggctg
    15241 gcgaagcgga agtttgattc gtgggcaagt aacgaattgc atgtctgaaa tcgctaagca
    15301 gcttagctat gatgagatta ttgctatatc gagatggttg gctactcaag ctgtagaagg
    15361 agaagctcaa aaagtgcttt cgcccgattt gataaagcga tgtcaatcga tttttctgga
    15421 aggagtagcg caagaatgag cccttattac ttttgtttta cttcgggttt tgctagaaag
    15481 tgcgctctag gttatctcgt attttttgtt ttgtttggag tgcaaataaa cctgatttct
    15541 gctgctccgg gagaacagga cttagcgcga ggtgaatatc ttacgcgagt tggaaattgt
    15601 ctaggctgtc atacagcaaa aggaggcgtt ccatttgcag gtggacggcg attgtcgact
    15661 gcatttggta cgtttgtgac gcccaatatt acgccggata aggaaactgg aattggccat
    15721 tggaccgaag aggatttttg gttagcgatg catgagggaa aatctcgaga tgggcgaatg
    15781 ctctatcctg catttcctta cactgaattt accaaggtat cacgagctga tgtaaagtcg
    15841 atttttcaat atcttcaatc gattgctgcg gtttcccagg ttaacccagc acatgagatt
    15901 ccttttcctt ataactctaa gcccttgtta tttgggtggc ggaagctttt ttttaaacaa
    15961 ggcatttatg aagcggatcc aacacaaaac gaagaatgga atcgtggcgc ctatctggtt
    16021 caaggattag ggcattgcga tgcgtgtcat acagcgcgta atgaagtcgg cgctaaaaaa
    16081 gatgctgcag ttggaggtgg gcaaatgatg ggcgcaaact ggtacgcacc ttctctttat
    16141 tcccgtaaag aggcgagtgt taccgactgg agtcttgaag cta
//
LOCUS       DAOQKQ010000042        15999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_1682_length_15999_cov_6.577898, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000042 DAOQKQ010000000
VERSION     DAOQKQ010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 15999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15999
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1682_length_15999_cov_6.577898"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..509
                     /locus_tag="PKZ49_04245"
     CDS             39..509
                     /locus_tag="PKZ49_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HND35805.1"
                     /translation="MNLVSQFLPESNVIIDLDVASKKRVFEQAGLLFENTSHVARSQV
                     FDSLFAREKLGSTGLGQGVAIPHGRIKGLREATVALVRMKEAIPFDAPDGLPVNIACI
                     LLVPEKATDKHLQILSELAQMFSDKSFREKLLMSKNTKEIHQLITDWIPDNVPN"
     gene            496..1437
                     /gene="hprK"
                     /locus_tag="PKZ49_04250"
     CDS             496..1437
                     /gene="hprK"
                     /locus_tag="PKZ49_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HND35806.1"
                     /translation="MSQISITQLFEDNKEKLGLVWNLGLEYGSNHLSNDDTSCSSQGL
                     IGHLNFIHPNWIQILSNLTIDYLNQLDKASFQQKLDKLAQSSLACLVVADGATVPELL
                     QSFAIKTKIPLFCSSSSSLDVIWVLRSYLAKVLAPSTSKHGVLLDVLGMGVLITGDSG
                     VGKSELALELISRGHGLVADDVVEIHRIGPETLEGCCPPLLRDYLEVRGLGMLNIRTI
                     FGETAVRRRKNMKLIVHLEKTSAADIGSFERLPLSNLDEEILNVKIRKVTIPVAAGRN
                     LAVLVEAAVRNYILQLRGIDNTQEFIKRHEQEMAGDL"
     gene            1434..2051
                     /locus_tag="PKZ49_04255"
     CDS             1434..2051
                     /locus_tag="PKZ49_04255"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HND35807.1"
                     /translation="MIDKAPPRTITLAFTGASGMPYGIRLLEQLLAQHIRVYLLYSQV
                     AQIVAQQEMQLALPSRSQDAEHFFIDYFNVSKDLLKVFGREEWFAPVASGSNPAEAMV
                     VCPCTMGTLSAIASGLNQKLIERAADVMIKENRKLILVPREMPFSAIHLENMLRLARC
                     GVIILPANPGFYHHPKNIQDIVDFVVARILDHLGVEHSLIPRWGE"
     gene            2200..2703
                     /locus_tag="PKZ49_04260"
     CDS             2200..2703
                     /locus_tag="PKZ49_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCAM0308 family protein"
                     /protein_id="HND35808.1"
                     /translation="MSHSKILDGYRAIPRHDGIFKEEIHDTYQLKSKLAEPTVCSQCQ
                     AVYHQGRWQWLTAPASAHQGICPACHRIRDDYPAGYLTLQGVFYLEHREEIMHLVRNH
                     EKHQCTEHPLKRIMKIEESVDLTLVTTTDIHLVRGMGEAIHHAYQGDLEFRYNPEENR
                     LRVYWKR"
     gene            2861..3544
                     /gene="hda"
                     /locus_tag="PKZ49_04265"
     CDS             2861..3544
                     /gene="hda"
                     /locus_tag="PKZ49_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HND35809.1"
                     /translation="MKQLLLDLSLENIPTLENFIPGRNVELLHALNEFLATSSSGNFI
                     YCWGGSGSGKSHLLKSVCDACIRLKRYQVAYFACDQYTEFELSDDLPGCIIVDDVDRL
                     GPEAQIKLFNLYNQARDDCLALFLVSGKTVPTQLQLRQDVITRLSWGLVYQIHELTDD
                     EKGKAMKNHAVNNGFELPAEVCDYLLHYEKRDMPYLIKMIDALGRYSLMLQRPITLPL
                     LRQFLNEVL"
     gene            3541..4203
                     /locus_tag="PKZ49_04270"
     CDS             3541..4203
                     /locus_tag="PKZ49_04270"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HND35810.1"
                     /translation="MKLALFDLDNTLLAGDSDFQWAQFLIEQKVLDREVYEARNIEFY
                     EQYKAGTLDIHEFLDFQLKPLARHPREQLNTWREEFIQKKIRPIIAPGALELIAKHKL
                     ERNLCIIITATNSFVTSPIARILGVDHLIATEPEQKEGQFTGRVSGMPCFREGKITRL
                     EQWLDAHNLTWLSFLESWFYSDSLNDLPLLRKVTHPVAVDPDATLENHARQNDWPIIS
                     LR"
     gene            4805..5221
                     /locus_tag="PKZ49_04275"
     CDS             4805..5221
                     /locus_tag="PKZ49_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35811.1"
                     /translation="MFSMMVDNKSKSFVLIILVLLISGSFSEVSATHISRPILSEART
                     EYSQGEKIVVNGWVNYQDQSTPDVLLNAKLLRADGTEVIEVFGISDKQGKFSFEFETN
                     RLLPGSYQIIVTSQCLEVHRPVCTYQSSTLAIDMKE"
     gene            5334..6719
                     /locus_tag="PKZ49_04280"
     CDS             5334..6719
                     /locus_tag="PKZ49_04280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35812.1"
                     /translation="MSLNIAETVILPCETKSREFDAKLLLACFIAEKGIKAIVGAKKV
                     IDGLALSLPRGIYIGKSLTAKSATVLRMLRQLGYTVVAWDEEGLVWATPELYRLTKLD
                     SRTLKELVGLFAWGEANASAIREHPGFSNIPIYLTGNPRTDLLRPELRGLFTQEVEQL
                     IRRYGRFILINTNFSRVNHYYANESHLLNQLRRDENTNLVRNDLKIGLARHKKQLFDH
                     FIEVLPQLADHFPEINIIVRPHPSENHQAWYEAANHKQNVKIIYEGNVVPWIMAAETI
                     IHNSCTTAVESFLLDKIALAYCPVKSEVFDHALPNGLSVQCDNFQQLVEKVANSLQGN
                     SPMNSINRMQYHDLAKQHLASFERTFASERIAQILQELCKKPCRKVPLFSRMAGLGTA
                     YWRSFIKKRIEAVIPNHRNNTTYLRHLFPMTSLQEVENKVNQVSSLLGRFQHLHVNNI
                     AENVFQIEARH"
     gene            6763..7848
                     /locus_tag="PKZ49_04285"
     CDS             6763..7848
                     /locus_tag="PKZ49_04285"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HND35813.1"
                     /translation="MKILGAGSIGNHLAYACRQLGWNVTICDIDPAALRRTRDKIFPG
                     RYGKWDETIRLVQPAEVADEAFEVIIIGTPPDSHIDLALGCLAKTPPQVLLIEKPLCT
                     PLLQSCEQLIPIAKRVGTRVLVGYNHTLTSNTLLAEEWLQEGKLGKILTIDVQIREHW
                     QGIFKAHPWISGPEETYLGYTQRGGGALAEHSHGLAIWQHFARLSGNGRIIEVSAMLD
                     MVNEAGAYYDRLAQLSLRTETGLTGLLVQDVITEPAKKYLRILGSGGYLDWFINIKPG
                     ADAVKLWSQDDVERAQYFEKTRPDDFLPEINHIERILSNPALSSPISLEYGLETMLVI
                     AAAIKSHQERRVIKIDYTRPYGLESLT"
     gene            7869..8936
                     /locus_tag="PKZ49_04290"
     CDS             7869..8936
                     /locus_tag="PKZ49_04290"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HND35814.1"
                     /translation="MLSHQKILVVVPARGGSKGVPLKNLRKIGGISLVERVGLLIQKL
                     DWVDRAVVSTDHPEIAKVAEGAGLAVPFWRPVELAGDQVGDIPVLQHALHATEAMDET
                     QYDIIVMLQPTSPLRRAEHVVATVEKLIAGNFDAVWTVSPTDLKYHPLKQLSLDDKGA
                     LNLWDKEGAHIVARQQLHQTYYRNGAAYALTRDCLLNQASLLGAHSFAVIIPEFMLSI
                     DTEEDFAIVEKYLAMRENQHDLNLNNAVINKTHSLGSKVFVIDIDGVIASLIANNDYA
                     LCQPISKNIEMINALYRAGHRIVLFTARGSMTGIDWSEITRQQLTDWQVLHHDLKFGK
                     PAADFYIDDRMILLDETSRFI"
     gene            8994..10493
                     /locus_tag="PKZ49_04295"
     CDS             8994..10493
                     /locus_tag="PKZ49_04295"
                     /inference="COORDINATES: protein motif:HMM:NF015087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HND35815.1"
                     /translation="MGELFDRLYIFEMANNHEGSVAHGLKIIDVVANLAKKHQIKAAV
                     KLQLRELETFIHPDYKQREDIKHIKRFESARLNFQDFEELVEAIHHHHLISVATPFDE
                     SSVANCQKLGIQILKLASCSATDWPLMTAMAEVKKPIIASTGGLGISDIDHLVSFFYK
                     RVPAFALMHCVSIYPTPNDRIHMNFLGKMIRRYPYVKVGYSGHESPENRMGVMAAVAK
                     GAQLLERHIGVSAEGIKLNRYSLDPDQADHWVSAAEEMREICGGDDHKFIGEDEIVSL
                     RELQRGVYVKQQVKQGTPILDDDVFFAMPCAEGQLTSGEFGQLRAKFIATQDYVPGAA
                     LYEKTEQDSIRMVREIVHDTKGLLYEAGIELGKDYQIEISHHYGLSKFREMGCVLINL
                     INREYCYKLLIMLPGQKHPPHLHQQKEETFYLLWGDITVNLRDSGEVSMKPGDRLLIH
                     RGILHDFMTKSGAIVAEISTHSIVGDSYYENKEIARLDPIQRKTFVESW"
     gene            10796..12634
                     /locus_tag="PKZ49_04300"
     CDS             10796..12634
                     /locus_tag="PKZ49_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HND35816.1"
                     /translation="MSSTIIKLGDPTMQESDFHPSDEVSVKMIPTPTLNELSQRLADF
                     ETLTDALEYAAKGVTGYNFYDAKGGLRSVLPYRTLKVNAQSKARRLLSLGFSHGSRVA
                     LIADTSPEFIELFFACRYAGLVPFAMPIPVNLGSHSIYVRQLQGMLESSGASAAIANL
                     DYLSFLKEAGDGLSNGHLKWVGTSAQLDSLPNSDKALKSNTPSETAYLQFTSGSTRMP
                     RGVVITEEALMCNLRGIVRNGLDVNPGDRCASWLPFYHDMGLVGLVLSPMAAQLTVDY
                     LATRDFAIRPLQWLKLISRNRCTIAFSQPFGLKLCALRVRDSDLEELDLSCWRAAGIG
                     AEMIRPEILRNFADKFASAGFSDRAFLPCYGLAESTLAVSFTKINRGIESLKVDTKTL
                     VEKRMAVRVQAEGRKFNEFVNCGRPLPEHTVKIVDENGQELPQMMVGSVLVKGGSIMT
                     GYFNNQEETDKALKSGGWLDTGDIGFLFDGDLYITGRKTDVIIVNGRNIRAQDIEELA
                     EQQSEVRSREASAFGISDSNGATTVVLVVECRLTSMEERQLLINRLQQLVYMAFGVNC
                     LVELVPPHTLPRTSSGKLSRFAARQGFLQRANMIDQLPTASLSGDR"
     gene            12641..13573
                     /locus_tag="PKZ49_04305"
     CDS             12641..13573
                     /locus_tag="PKZ49_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HND35817.1"
                     /translation="MHQVVAVTGATGFIGQNLLDALIHDGWLVNALTRKQPSKNHHKN
                     IKWVLGDLDDHTALLELVTRASAIIHCAGLVRGNSPEVFFHANAIGTENLLKAIASVK
                     LNPRFLLISSLAAREPTLSWYAESKSKAEQIALHYLHDIGISYAIFRPSAVYGPGDKE
                     LLPVFQASRYGIVPVVGNFENRFGLLHVSDLVELICYWLNNESFTNGTYEIDDATPGG
                     YSYQSVAEIAQQILHRPVRCIKLPGKLIQQIASMNLWLSKFLNYSPMLTPGKFRELQH
                     PNWVCDMSLFKQKGINWAPHNSLREALPNLIKSK"
     gene            13578..13835
                     /locus_tag="PKZ49_04310"
     CDS             13578..13835
                     /locus_tag="PKZ49_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HND35818.1"
                     /translation="MNEVTQEGILKLLCQRLTGLTKADFEITSETNLITQLAIDSIKL
                     LNLIMEIEDNFDISVPVNALTDVITVRDLADLIYRIKSESK"
     gene            13832..15016
                     /locus_tag="PKZ49_04315"
     CDS             13832..15016
                     /locus_tag="PKZ49_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HND35819.1"
                     /translation="MSLLEKLDQAAAARKAQLPGGIGAFGIPIEAIYSATEARIDGRK
                     ILLFGTNNYLGLSFSSECREAAHAAIDEEGTGTTGSRMANGNYSGHRALEAEFADFYH
                     CRSSIVFTTGYQANLGTISALLGKGDVALIDGDSHASIYDGCILSGADIIRFRHNDKD
                     DLEKRLRRLGERSKNTLIIAEGIYSMLGDQAPLVDIVNLKKQYGSLLLLDEAHSLGVL
                     GETGQGLVEKTGLNHEVDFITGTFSKSLCGIGGYCVSNHEQLDQMRYVSHPYIFTASP
                     SPATIAATRAALKILRTATGLRKALWQNAEQLYSALKDIGYQLGPQPGPIVAIILDNP
                     EQALALWNGLMTANIYVNLILPPAAPEGKSLVRCSINAAHTPEQIHYVSQVLSELYAK
                     LH"
     gene            15086..15844
                     /gene="pgeF"
                     /locus_tag="PKZ49_04320"
     CDS             15086..15844
                     /gene="pgeF"
                     /locus_tag="PKZ49_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HND35820.1"
                     /translation="MKDWITPEWPSPANVRALFTTRQGGVSQDVEGKYAGLNLGDHVE
                     DDHALVQQNRQLLRNYLPSDPCWLKQIHGSRFVWVDALKSEHHDFEADAALSRTQGVV
                     CAVLVADCLPILLCNRGGSIVGAIHAGWRGLASGVIENAVYGMNCKTSDLIAWLGPAI
                     SNQYFEVGEEVRDTFVNQDGQSSLAFFAATDTGKWFADIFMLAKQRLVNLGINQIYGG
                     GECTYSNPSQFYSYRRDKRTGRMAALIWLSALNV"
BASE COUNT         4666 a         2822 c         3682 g         4829 t
ORIGIN      
        1 taagtagtag ttgtttaaag taactgttta tttttcttat gaatcttgtt tctcagtttt
       61 tacctgaatc gaatgtaatt attgatctcg acgttgctag caagaaacgt gtatttgagc
      121 aagcagggtt attatttgag aatacttcgc atgtggctcg gagtcaggtg tttgatagct
      181 tatttgcccg agagaagctt ggatcaacgg gcctgggtca gggcgttgct attccacatg
      241 gtagaattaa agggttgcgg gaagcaacgg ttgcattggt gagaatgaaa gaagcgattc
      301 cgtttgatgc gcccgatgga ttgccggtaa atatcgcttg tatactactt gtacctgaaa
      361 aagcgacaga taagcattta caaattttaa gtgaattggc gcaaatgttt agtgataaaa
      421 gctttcgtga aaaattactt atgagtaaaa atactaagga aattcatcag ctgattactg
      481 attggatacc tgataatgtc ccaaattagt atcacgcaat tatttgaaga taataaagaa
      541 aagcttggct tggtttggaa tttgggactg gagtatggta gcaaccattt gagtaatgat
      601 gatacctcat gttctagtca aggattaatc ggccatctca attttattca ccctaattgg
      661 attcagatac tcagtaacct taccattgat tatctgaatc agctcgataa agcctctttt
      721 caacaaaaac ttgataaact agcgcagagt agtttggctt gtttggttgt agctgatggg
      781 gcgaccgtac ctgaattatt acaatcattt gcgatcaaga ccaagattcc tttattctgc
      841 tcatcttcat ccagtttgga tgttatctgg gtactacgat cttatttagc aaaagttcta
      901 gcaccaagca caagtaagca tggtgtattg ctcgacgtgc tgggaatggg tgtgcttatt
      961 accggtgata gcggggttgg gaaaagtgaa cttgctttag aattgataag tcgtggccat
     1021 ggtttagttg cagatgatgt tgtagaaatc catcgtatcg gtccagaaac attggaaggg
     1081 tgttgccccc cgttgcttcg tgattatctt gaagttaggg ggctcggcat gctaaatatt
     1141 cgtactatct ttggtgagac agccgttcgt cggcggaaga atatgaagct aattgttcat
     1201 ttagaaaaaa cgagtgctgc agacatcggt tcttttgaac gtttaccctt gagtaacctt
     1261 gatgaagaaa ttttgaatgt gaaaattcgg aaagtaacca tccctgttgc ggctggccgt
     1321 aatttagcag tattagtaga agctgcggtc cgtaactata ttctacagct cagagggatt
     1381 gacaatacgc aggaatttat taagcgccat gagcaggaga tggcagggga tctgtgatag
     1441 ataaggcacc accacgcaca attacacttg ctttcacagg agcatcggga atgccttacg
     1501 gtatccgatt gctggagcaa ttgttagcgc agcatatacg ggtttatttg ttgtattcac
     1561 aagtagccca aattgttgcg caacaggaaa tgcagctggc gctaccctct cgttctcaag
     1621 atgcagagca ttttttcatt gactacttta acgtatccaa agatctgctc aaggtatttg
     1681 gccgtgaaga gtggtttgcg cccgttgcat caggttcgaa tccagcagaa gcgatggttg
     1741 tttgtccttg tacgatgggc acgctttctg ctatcgcttc gggattgaat caaaagttaa
     1801 ttgagcgtgc agcagacgtt atgattaaag aaaatcgcaa actaatttta gtaccccgtg
     1861 aaatgccatt ttcagcgatt catctagaga atatgcttag acttgcgcgg tgtggtgtca
     1921 tcattttgcc tgccaatcct ggtttttacc atcaccccaa aaatatccaa gacatcgttg
     1981 attttgtagt cgctagaata ctcgatcatc ttggtgttga gcattccctg atcccgcgct
     2041 ggggagaata aaagtaattg attttttctt atatgcccct atatacaaat atagcttaag
     2101 gtcaatgaat tgtgcttgtt gctcttagtc tgtttaaaat agagcaaggt tttatgtaag
     2161 aaaagatgga atgtcaatta ttaagatagg agagctaaga tgagtcactc gaagattcta
     2221 gatggatacc gtgccattcc tcgccatgat ggcatattta aagaagaaat acacgatacc
     2281 tatcaattaa aaagcaaatt agctgaacca acagtttgtt cacagtgtca ggcggtatac
     2341 catcaaggtc ggtggcagtg gttaacagcc ccagctagtg cacatcaagg aatttgccca
     2401 gcatgtcatc gtattcggga tgattacccc gccggttatt tgactttgca gggagtgttt
     2461 tatttagagc atcgagaaga aattatgcat ttagtgagaa atcacgagaa acaccagtgt
     2521 acagagcatc cacttaaacg catcatgaaa attgaggaga gcgttgactt aacccttgta
     2581 acgacaactg atattcatct tgtgcgagga atgggagaag cgattcatca tgcttatcag
     2641 ggggatctgg aatttcgcta taacccagaa gagaataggt tgagagtata ttggaaacgt
     2701 taatttccaa gttgtggaaa aaattctctg tttaatatgt gccgtagcgc aaaaaaagta
     2761 agtagtaagt aaaaaattag ttatcttggt tgctattttg ttttcatggt acttactgta
     2821 tttaatatcg ctacggaaga actgatcagg gaatcgctca atgaagcaat tgttgcttga
     2881 tctttcccta gaaaatattc caacgttaga aaattttatt ccaggacgta atgttgagtt
     2941 gcttcatgcc ttgaatgagt ttctagccac atcgagttct ggtaatttta tctattgctg
     3001 gggtggaagt ggatctggta aaagtcattt gctcaaatca gtttgtgatg cttgtataag
     3061 attaaaacgc tatcaagtgg cttatttcgc atgtgatcaa tatactgaat ttgaactcag
     3121 tgacgattta ccgggttgta ttatcgttga tgatgttgat cggttaggcc cagaagcgca
     3181 aataaaactg tttaatttgt ataatcaggc acgtgatgat tgcctggcgc tgtttttggt
     3241 gagtggtaaa acagttccaa cccaacttca gctccggcaa gatgtaataa cccgcttgag
     3301 ctggggattg gtttaccaga ttcatgagct aacagatgat gagaaaggta aggcgatgaa
     3361 gaatcatgcg gttaataatg gattcgaatt gcctgcagag gtttgtgatt atttgttgca
     3421 ctacgagaaa cgagatatgc cgtatctgat caaaatgatc gatgccctgg gtcgatattc
     3481 actgatgctg cagcgtccga ttacacttcc gctattacga caatttttaa atgaggtgtt
     3541 atgaaactag ctttgtttga tcttgataat acattactag ctggcgatag cgatttccag
     3601 tgggcccagt ttttaatcga acagaaagta ttggatcgtg aagtttatga agcgcgcaat
     3661 attgaattct atgagcaata caaggcgggt actctcgata ttcacgaatt tttagatttt
     3721 caacttaaac cccttgcacg acatccgaga gaacaattga acacttggcg cgaggaattt
     3781 atccaaaaaa aaattaggcc gatcattgcg ccgggtgcgc ttgagttgat cgcaaaacat
     3841 aagcttgaac ggaatctttg tatcatcatt acagcaacga atagctttgt gacttcgccc
     3901 atagcaagaa tactgggtgt agatcattta attgcaacag aaccagagca aaaagagggt
     3961 caatttacag gccgggtttc tggtatgcct tgttttaggg aaggaaagat tacgaggtta
     4021 gagcaatggc ttgatgcaca caatcttact tggttatcct ttcttgaaag ttggttttat
     4081 agtgattctt tgaatgattt accactctta aggaaagtaa cacatccagt tgcggttgat
     4141 cccgatgcaa cacttgagaa tcacgcacga caaaatgatt ggccaatcat cagtcttcga
     4201 taattagttt catagaacta caaattagat gcgtcactta ttattgctat gttgacaagc
     4261 gtcaatagcg atttttggac atttactatt actaaaaaaa acaccacgga cctaaccgtg
     4321 gtgcattcca gtgtttatac aaaagatgct ttgtgatctt ttgtaagtgc tcagctttca
     4381 atttgatcca ttattttcta ggaactatta ttattttcat cttatttcgc ataaatttgc
     4441 gttgttggat tcatttattc ctaagcacgt atattcataa gcaaacctca tgccataacg
     4501 ctgaaagcta ttatttctgt taggatcaag tcattgtcag tgcataagaa aaatcgtatg
     4561 taagaaatat cgacaatata taaatcgata agataagcaa ggaagaaagc ataaaatatt
     4621 aattttgttt gaatttagaa tggttttgct gagtaattgt tagcgcatac gatccgtaag
     4681 ttaatgttca tgtgtaagaa aaatcgacaa ctaattttcg attaagctat agttaggaaa
     4741 gtaagaaaaa aagaaagtgt acggaatagt ttaatattta tagtaaaagg aaataagaaa
     4801 agccatgttt agcatgatgg ttgataataa aagtaaaagt tttgtattaa ttattctggt
     4861 tttattaatc agcggctcgt tttctgaagt tagtgcaacc catatctcta ggccgatttt
     4921 atcggaagct agaacagaat attcccaagg ggaaaaaatt gtagtaaatg ggtgggttaa
     4981 ttatcaggat cagtctacac ctgatgtttt gttaaatgct aaattgcttc gtgcagatgg
     5041 aacagaagtc attgaagtgt ttggtatcag cgacaaacag ggtaaattta gttttgagtt
     5101 tgaaaccaat cgtttacttc ccggtagcta tcagataatc gtcacctctc aatgtttaga
     5161 agtgcatcgg cctgtttgta cttatcagag ctcaacgctt gcaattgata tgaaagaata
     5221 agtttcaacg atcttgccaa agccccaccc agtattgcca atacacgatg agattttttt
     5281 ttgattagaa tctaaatctt ttttccaaaa agtttatcgt tacgttttcg tgtatgagtc
     5341 taaatattgc agagactgtt attttaccgt gtgagactaa atcacgagag tttgatgcaa
     5401 aactactatt ggcctgtttt attgctgaga aggggataaa ggctattgtg ggtgcaaaga
     5461 aagttataga tgggttggcg ctatcgttac ccagaggtat ttatatcgga aaaagcttga
     5521 ctgcgaaaag cgctacagtt ttgcgtatgt tgaggcaatt aggctatacc gttgtcgcat
     5581 gggacgagga agggctagtg tgggctacgc cagagttgta ccgattgaca aagctggatt
     5641 ctcggacact gaaagaactt gtcggtttgt ttgcatgggg cgaagcaaat gcatcggcta
     5701 tacgtgagca tcctgggttt tctaatattc cgatatatct gacaggaaat ccacggactg
     5761 atctactgcg tcctgaattg agagggttat ttactcaaga agtagagcaa ttaatccggc
     5821 gctatggtcg atttatactt attaatacca atttctcgcg cgtgaatcat tattatgcaa
     5881 acgagagcca tttacttaat cagttaagaa gagatgaaaa tacaaatctc gttcgaaatg
     5941 atttgaaaat agggcttgca aggcataaaa agcaactctt tgatcatttt atcgaagtgc
     6001 ttccacagtt ggcagatcac tttcctgaaa taaacattat cgtgcgacct catccatctg
     6061 agaatcatca ggcttggtat gaagcagcaa atcataaaca gaatgtgaaa attatctatg
     6121 aaggcaatgt tgttccttgg ataatggctg cagaaacgat tatccataat agttgtacga
     6181 cagcggtgga gtctttttta cttgataaga tagcacttgc ttattgcccc gtaaaatcag
     6241 aggtatttga tcatgcacta ccgaacggac tgagtgtgca atgtgacaat tttcagcaat
     6301 tagtagaaaa agtagcaaat tcgcttcaag gaaattcccc gatgaattcc attaaccgaa
     6361 tgcaatatca tgacttagca aagcagcatc tagcttcttt tgagcgtaca tttgcaagtg
     6421 aacggatcgc ccaaattctc caagaattat gcaaaaaacc atgccgtaaa gttcctttgt
     6481 tttcaagaat ggcgggcctt ggcactgcct attggcgttc atttatcaag aagcgaattg
     6541 aagcagttat accgaatcat cgtaacaata cgacttattt gaggcattta tttccaatga
     6601 cctctttgca agaagttgag aataaagtca atcaagtgag ttcattatta ggacggttcc
     6661 agcacctgca tgtgaataat attgctgaga atgtatttca gattgaagct aggcattaag
     6721 aatacaggta attagagtgt gctctgaaat atggaagtta aagtgaaaat attaggcgca
     6781 ggttcgattg gtaatcacct tgcctatgct tgccgccagt taggttggaa cgttacgatc
     6841 tgtgatattg atcctgcagc gcttaggcgt acacgcgata aaatttttcc tggtcgatat
     6901 ggaaaatggg atgaaacaat tcgtctagtg caacccgctg aggtagcgga cgaagctttt
     6961 gaagtgatca ttatcggaac tcctcccgat agccacattg atcttgcgct ggggtgtctt
     7021 gcaaaaacac caccacaagt attgttgatt gaaaaaccct tatgtacgcc ccttctacaa
     7081 agttgtgaac aattaattcc tatagctaaa cgggttggaa cccgtgtctt ggttggttac
     7141 aaccatactt tgactagtaa tacgttgctc gcagaggaat ggcttcaaga ggggaagctt
     7201 ggaaaaattt tgacgattga tgtgcagatt cgtgagcatt ggcagggtat ctttaaagcg
     7261 catccctgga tttctggacc cgaggaaacc tatctcggtt atacccaacg gggaggaggt
     7321 gcactagcag aacattctca tggtttagcc atttggcagc actttgctcg cttgagcggg
     7381 aatggtcgta tcattgaagt gtctgcgatg cttgatatgg tgaacgaagc gggagcgtat
     7441 tatgatcgtt tggctcagtt aagtttgcgg actgagaccg gattgaccgg attgttagtg
     7501 caagacgtga tcacagagcc agcaaaaaaa tatttaagga ttctgggttc aggaggatat
     7561 ttagattggt ttattaatat aaagccaggc gctgatgctg ttaaattgtg gtcacaggat
     7621 gacgtagagc gcgcccagta ttttgagaaa acgcgacccg atgatttttt accagaaatc
     7681 aaccatattg aacgtatctt atcgaatcca gcgctttcat ctccaatctc tctggagtac
     7741 ggtttagaga cgatgcttgt tattgcagcg gctatcaaat cccatcaaga aaggcgtgtg
     7801 atcaagattg attatacgcg cccctatgga ctcgaatcgt taacatgagt tgaactcggg
     7861 attctattgt gctatctcat caaaaaattc tagttgttgt tcctgctcgt ggtggaagta
     7921 aaggcgtgcc actaaagaat ttgcgcaaaa taggcggcat ttctttggtc gaacgagttg
     7981 gtttattgat tcagaaatta gattgggttg atcgtgccgt ggtttcaacc gatcatcctg
     8041 aaatcgcaaa ggtcgcagag ggggctggac tggctgtgcc attctggcgt ccagtagaac
     8101 tggcgggaga ccaagtggga gatatccctg tgctccagca tgcgctccat gctacggagg
     8161 cgatggacga gactcagtat gatattatcg tcatgttgca acccacatca ccgctaaggc
     8221 gagcggagca tgttgttgca acggtagaga agctgattgc agggaatttt gatgcagttt
     8281 ggactgtaag tcctactgat ttaaaatatc atccgctaaa acagctaagc ttagatgata
     8341 agggagcatt aaatctatgg gataaggaag gtgctcatat cgttgcgcga cagcagcttc
     8401 atcaaaccta ttatcgcaat ggcgcagcct acgcccttac acgcgattgt ttgctcaacc
     8461 aagcttcact actgggggcc catagctttg cagtgattat ccctgaattc atgctaagta
     8521 ttgatacgga agaagatttt gcaattgtgg aaaaatacct tgcgatgagg gaaaaccagc
     8581 atgatcttaa tcttaataat gcggttatta acaaaacaca ttctcttggt tccaaagtgt
     8641 ttgtaattga cattgatggc gttattgctt cactcattgc gaataatgac tatgcgcttt
     8701 gtcagccaat cagcaaaaat attgaaatga ttaatgcctt atatcgggcg ggacatcgaa
     8761 ttgtattatt tacagcgcga ggctcaatga ccggtattga ctggtctgaa ataacccgac
     8821 aacagttaac agattggcaa gtgcttcatc atgatttgaa atttggtaag ccggctgctg
     8881 atttttatat cgatgatcga atgattttat tggatgaaac gagccgcttt atctaagtca
     8941 gtagcgtaac taaaaagtaa taaataagta ataaatgtga tttgatactg agtatgggtg
     9001 aactatttga tcggctgtat atttttgaaa tggctaataa ccatgagggt tctgtagcgc
     9061 atggattaaa aattatcgat gtcgttgcta atttagccaa gaagcaccaa attaaagcgg
     9121 cagttaaatt acaattacgc gaattagaaa ccttcattca tcctgattac aagcagcgag
     9181 aggacatcaa gcatattaaa cgttttgaga gtgcgcgact aaattttcag gatttcgagg
     9241 aactcgtaga ggctatccac catcatcatc ttatttcagt tgcaactccc ttcgatgagt
     9301 catctgtagc taattgtcaa aaattaggaa tccaaatctt aaaattggca agttgttcag
     9361 ctaccgattg gcctctgatg acagcaatgg cagaagtaaa aaagccaatt attgcctcga
     9421 ccggggggct cggtatctct gatattgatc atttagtcag ttttttttat aaacgagtgc
     9481 cagcgtttgc attgatgcat tgtgtttcaa tttacccgac accgaatgat cgtattcaca
     9541 tgaatttttt aggaaaaatg attcgtcgtt atccttacgt gaaagtaggt tattcaggac
     9601 atgagtcacc cgaaaataga atgggggtaa tggcagcggt ggcaaagggc gctcaattac
     9661 ttgagcgcca tattggcgtg tcagcggagg gtattaaatt gaatcgctat tctttagacc
     9721 ccgatcaggc tgatcattgg gtatcggctg cagaagagat gagagaaatc tgtggaggtg
     9781 atgatcataa gtttatcggt gaagacgaaa tagtttcgtt gcgcgagctt cagcgagggg
     9841 tctatgtaaa gcaacaggta aaacaaggta caccgattct tgatgatgac gttttttttg
     9901 cgatgccatg tgctgagggt cagttgacta gcggggaatt tgggcagctt cgcgcaaaat
     9961 ttattgccac acaggactat gtcccaggag cggctctcta tgagaaaacc gaacaagact
    10021 ccattcgaat ggtaagggag attgtacatg atacaaaagg tttgctttat gaagctggga
    10081 ttgaattagg gaaggattat caaattgaaa tttcccatca ttatggtttg tcaaaatttc
    10141 gagaaatggg atgtgttttg atcaatctga taaatcgaga atactgttat aagctactta
    10201 tcatgttgcc gggtcagaaa catcctccgc acttgcatca acagaaagaa gaaacttttt
    10261 atttattatg gggcgatata accgtcaatc ttcgagattc gggtgaggtt agcatgaagc
    10321 ctggcgatag gctgttgata catagaggaa tattgcatga tttcatgacg aaatctgggg
    10381 cgattgtggc tgagatttcg acgcatagca ttgttggaga ttcctactat gaaaacaaag
    10441 aaatagcacg acttgatccg attcagcgaa aaacatttgt tgagagttgg tagtagatta
    10501 ttggtttcat tgtttattta ctgcttttca gattcttaat aaatagtttg tgaactgtat
    10561 caaataaata aaatttccga atcgagctgt tgctattaaa caacattcac ataatatcgt
    10621 cttgacagga aaatcaccct tatataagct ccaacgtcaa cgaatatgag acacttgtgt
    10681 ttatttagca acgaaataac agtatattta aaccatatcc aggttgggtt agcgtcagta
    10741 tctgtcgtgc ttataaacat aagatgacca gcactattac ttctagggga agatcttgag
    10801 ttcgacaata atcaagctgg gtgatccaac aatgcaagaa tctgattttc atccttcaga
    10861 cgaagtatct gtaaaaatga tacctacgcc aacgcttaat gaattaagtc aaagattggc
    10921 tgattttgag acattaacag atgctttaga gtatgccgcg aagggtgtta cgggctataa
    10981 tttttatgat gcgaagggag ggctgcgctc agtacttcct tatcgtacac ttaaagtgaa
    11041 tgcgcaatct aaggcaagga gattgctgtc actgggtttc tcacatggta gccgggttgc
    11101 gcttattgct gatacatcgc ccgagtttat cgagttgttt tttgcttgcc gctatgcggg
    11161 tttagttccg tttgcaatgc ctatccctgt taatctgggt agtcattcga tctatgttcg
    11221 ccaattacag ggaatgcttg agagtagtgg tgcaagtgct gcaattgcga atcttgatta
    11281 tctaagcttt cttaaagaag caggtgatgg attaagcaat ggccatctaa aatgggttgg
    11341 aacatcagct cagttggact cgcttcctaa ttcagataag gcacttaaat ctaatacacc
    11401 gagcgaaaca gcatatttgc aatttacttc tggtagtacc cgtatgccac ggggtgttgt
    11461 aattacggaa gaagctttga tgtgtaatct acgtggtatc gttcgtaatg gtttagatgt
    11521 taatccaggc gatcgctgcg cttcatggtt accgttttat catgacatgg gcttagttgg
    11581 acttgtgttg tctcctatgg ctgctcaact tacggtcgat tatttggcta cacgagattt
    11641 tgcaattaga cctttacagt ggttaaagtt gataagtcgt aatcgctgca caattgcatt
    11701 tagccaacct tttggcctta aactttgtgc tcttcgtgtt cgagattctg atttggaaga
    11761 attagacctc agttgctggc gtgctgcggg cattggagca gaaatgattc gtccagaaat
    11821 tttgaggaat ttcgcagata aatttgcttc agctggattt agtgatcgtg cttttttacc
    11881 ttgctatggt ttggctgaat caacacttgc cgtatcgttt accaagatta atcgtggtat
    11941 tgagagcttg aaggtcgata ccaaaacgct cgtggaaaaa agaatggcgg tgcgagtgca
    12001 agctgaaggt aggaagttta atgaattcgt taattgtggt cggcctctgc cagagcatac
    12061 agtaaagatt gtagatgaga atggtcaaga gttaccacaa atgatggttg gtagtgtact
    12121 tgtcaaaggc ggtagcatta tgacaggata cttcaataat caagaagaaa ccgataaagc
    12181 tttaaaatca ggtggttggc ttgatactgg tgatattggt tttttatttg atggcgatct
    12241 ctatataaca ggacgcaaaa cggatgttat tatcgttaac ggtagaaata tacgtgctca
    12301 agatattgaa gaattagctg aacaacaatc agaagttaga tcgcgcgaag catcggcatt
    12361 tggtatcagt gactcaaatg gcgcaacaac tgttgtatta gtcgttgaat gtcgtctcac
    12421 ttcgatggaa gaacgacaat tactgataaa tcgtttacaa cagttagtgt atatggcatt
    12481 tggggttaat tgcttagttg agctggttcc tcctcataca ctgcctagga cttcatcagg
    12541 taaattgtct cgatttgcag cacgccaggg attcttgcag cgtgcgaata tgatcgatca
    12601 attaccaaca gcttcattat ctggagatag ataattctaa atgcatcaag tagtggcggt
    12661 tacaggtgcc acagggttta ttgggcagaa tctacttgat gcattaatcc atgatgggtg
    12721 gttagtaaac gcactcacta gaaagcaacc atctaaaaat catcataaaa atatcaaatg
    12781 ggttttgggt gatcttgatg atcacactgc gcttctagag cttgttacga gagcttctgc
    12841 aattattcat tgtgctggtt tggttagggg aaactcgcca gaggttttct tccacgctaa
    12901 tgcgataggt actgagaatt tactgaaagc tatagcatca gtaaagctta accctcgttt
    12961 tctcctgatt tcatcccttg ctgcaagaga accgacattg tcatggtatg cagagagtaa
    13021 atctaaagca gaacagatcg cgcttcatta tttgcacgat atcggtattt catatgctat
    13081 ttttcgacca tcagctgttt acggacccgg tgataaagag ttgttaccgg tattccaggc
    13141 atctcgctat ggtatcgtac ctgtagttgg taatttcgag aatcggtttg gtttgctaca
    13201 tgtcagtgat ttagtcgagc ttatttgtta ttggttaaat aatgaaagct ttacgaatgg
    13261 aacttacgag attgacgatg caacacctgg cggatatagt taccaatctg tcgcagaaat
    13321 cgctcagcag attctccata gaccggttag gtgtattaaa ttacctggaa agttaataca
    13381 gcagattgca agcatgaatt tatggctatc taagtttctg aattattcgc ccatgctgac
    13441 accaggtaaa tttcgtgaat tacagcatcc gaactgggta tgtgatatga gtttgtttaa
    13501 gcagaaggga atcaactggg ctccccataa ttcgcttcgc gaagcgctcc ccaatttaat
    13561 aaaatctaaa tagaactatg aatgaagtga cgcaagaagg aatcctaaag ctattatgcc
    13621 agcgattaac cggtctgact aaagcagact tcgagataac atcagaaact aatctcatta
    13681 cgcaattggc aatagattcg ataaagctat taaatttgat tatggaaatt gaggataatt
    13741 ttgatatttc agttcctgtt aatgcactta ccgatgtaat aactgttcga gacttagcgg
    13801 atttgattta tcgaattaaa tcggagtcga aatgagtctg ttagaaaagc tcgatcaagc
    13861 tgctgcagct agaaaagcac aattgccagg cgggattggt gcatttggta taccgataga
    13921 agccatttat tccgcgacgg aagcgcgtat tgacggtcgc aagatattgt tatttggaac
    13981 caataattac ctagggctga gtttttcatc agaatgccga gaagctgctc atgcagcgat
    14041 tgatgaagaa ggaactggaa cgacgggatc ccggatggcc aatggtaatt attctggaca
    14101 tcgagcgctt gaagctgagt tcgcagattt ttatcattgc cgttccagta tcgtttttac
    14161 tacgggttat caagcgaatc taggaactat ttcagcatta ttaggaaaag gcgatgttgc
    14221 gctcatcgat ggcgattcac atgccagtat ttacgatggt tgtatactca gtggtgcaga
    14281 tattatccgt ttccgccata atgataaaga tgacttagag aaacgattac gtcgcctggg
    14341 ggaacgaagt aaaaatacgc tcattattgc tgagggtatc tatagtatgt taggagacca
    14401 agcgcccctg gtcgacattg tcaatttaaa aaaacaatat ggcagcttac ttttattgga
    14461 tgaggcccat tccttagggg tgttaggaga aacagggcaa ggattagtcg agaaaacagg
    14521 actaaatcat gaagttgatt ttataactgg aacattcagt aagagtctct gtgggattgg
    14581 cggatattgt gttagtaatc atgagcagct tgatcaaatg cgctatgtga gtcatcccta
    14641 tatttttacg gcatctcctt ctcctgcaac cattgcggct actcgtgccg cattaaaaat
    14701 tttaagaact gcaacagggt taagaaaagc gctttggcag aatgcagaac agctctatag
    14761 cgcattaaaa gatattggat atcagttagg ccctcagcca ggacctattg tggcaattat
    14821 tctcgataat ccagagcaag cgcttgcttt atggaatggt ctcatgacag caaatattta
    14881 tgtgaattta attttgcccc ctgcggctcc tgaaggaaaa tcgttagttc gatgtagcat
    14941 taatgcggcc catacgcccg aacagattca ttacgtttca caagtgttat cagagttata
    15001 tgctaagttg cattagtttt attttacgta tatcgggaat tacttcattg ttttctatcc
    15061 gtctataaga tagattttct ctcgtatgaa agattggatt acaccagaat ggccatctcc
    15121 tgccaacgtg agagcgctat ttacaactcg gcaaggtggt gtgagtcagg atgttgaggg
    15181 gaagtatgct gggctgaatt taggtgatca tgttgaagat gatcatgcgc tagttcagca
    15241 aaaccggcaa ttattgagaa attatcttcc gagcgatcca tgctggctta aacagataca
    15301 tggatctaga tttgtctggg ttgatgcatt gaaaagtgag catcatgatt ttgaagccga
    15361 tgctgcctta tcgcggactc aaggggtcgt atgtgctgtt ttagtggccg actgtttacc
    15421 gatactgcta tgtaatcggg gaggaagcat tgttggggct attcatgcag gatggcgagg
    15481 gctagcaagt ggagtgatcg agaatgccgt ttatgggatg aattgcaaaa cttcagatct
    15541 catcgcttgg cttggtccgg caattagtaa tcagtatttt gaagtgggtg aagaagtaag
    15601 agacactttt gttaaccagg atggtcaatc cagcttagcc ttttttgctg caacagatac
    15661 tggaaaatgg tttgcagata tatttatgct agcaaaacaa agattggtta atttagggat
    15721 aaatcaaata tatggtgggg gtgaatgtac ttatagtaat ccctcccaat tttattccta
    15781 tcgacgtgat aaaagaactg gacggatggc tgcattaata tggttgtcag cgctaaatgt
    15841 ttgagttggc atacactgac tttggttaga tggtcatgac tgaaataagc tatattaaga
    15901 tttaattctg atcgtgaatg tatagatgaa tccagcactc ggatgtttac gtgctggatt
    15961 ttgttttttt gatagataat tctgaataat tttatgatt
//
LOCUS       DAOQKQ010000043        15989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_1683_length_15989_cov_5.915778, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000043 DAOQKQ010000000
VERSION     DAOQKQ010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 15989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15989
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1683_length_15989_cov_5.915778"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="PKZ49_04325"
     CDS             <1..344
                     /locus_tag="PKZ49_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HND35821.1"
                     /translation="VDKLTLIAAGTRHPHATELLASQSMADILMELAQRYSDRIVIFD
                     SPPLLLTSEARVLASQMGQIVLVVEAERTTQQTVKEALRQIESCDVINLIYNKARTSA
                     AAGYYGYYYSS"
     gene            341..1903
                     /locus_tag="PKZ49_04330"
     CDS             341..1903
                     /locus_tag="PKZ49_04330"
                     /inference="COORDINATES: protein motif:HMM:TIGR03016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03016 family PEP-CTERM system-associated
                     outer membrane protein"
                     /protein_id="HND35822.1"
                     /translation="MKLLSKHFCNYHYKGLVYISILLFNIIALPIYAGEWRVVPSLNI
                     NERYTDNLRLGGIATGGKGNDEFITQINPSILINGNARRYRVDVNYLMNNLIYANNSE
                     FNRIRHQLNANGTIELIQDRFFIDGRAVILQQNASLLGSQGLDNANPLNRRNMSMLNI
                     SPYYRHRFGDFATAELRYTHGEVNTSGGGLRDSSTDNGTVRLNSSTAFRDLQWGITYN
                     HTEIHRSQLRTIALERSTVLLRYMLTPQFSLVGTGGYERNSFISVNGGTSSPTWTVGF
                     SWIPNERTEFDFAGGQRIFGDTYLANFSHRTRLTTWSASYNEDITTFGQQNLLQPGAA
                     GAIPSGGILPQLLLNTLTNAGFGGGGLANQGALLGISGANNFFTNRLFLQRSLQAFMT
                     INGSRNTVVFRGFNISRQAYTGDEEDDSIGGRVNSVLNRNTVQTGGGVSWIYKLSPLM
                     NFTTNYTFTRINFTTTNLTDINQIMMVSLNKRFSPNLFGEISYFHNRRDSERINGDFS
                     SNNYMASLSMNF"
     gene            1929..2777
                     /locus_tag="PKZ49_04335"
     CDS             1929..2777
                     /locus_tag="PKZ49_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA-associated ATPase"
                     /protein_id="HND35823.1"
                     /translation="MYLSFYGLKTKPFQLKPDASFFFSSKGHKRAIAYLEYGISQEDG
                     FIIITGEVGAGKTTLIRHLFQKLDQQRFIVAQLANTNLDSDDMLRMVAASFGLPVTNT
                     SKATTLLEIEQLLRQYDKEGKKILLLVDEAQNLTSRALEELRMLSNFQTDDKLLLQTF
                     LLGQPEFRKTLLGNNMQQLRQRVIATYHLGPMDNTETRAYIEHRLAIAGWQGSPLLQD
                     DSFDVIYNFTGGIPRKINTLCDRLLIMGCLEELRTITSQEVNEVVADIQQEFDLPPFQ
                     NIETSD"
     gene            2877..3716
                     /locus_tag="PKZ49_04340"
     CDS             2877..3716
                     /locus_tag="PKZ49_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HND35824.1"
                     /translation="MIRNALTIDVEDYFQVSAFDPYIPRNDWDSIPCRVERNIDRILS
                     LLEKKGINATFFTLGWIAKRYPSMVRRIVENGHELASHGCDHDRVTELGSDEFYKDIA
                     DSKKILEDISGFPVIGYRAPSFSINKQNYWALNLLEEAGYLYSSSIYPVRHDHYGIPD
                     APRFAFYPNPTGKLLEVPITTVNLLGRNIPVGGGGYFRLWPYAVSRWFIQRVNEKDQR
                     SAVFYFHPWEIDPDQPRQKGINLKVRFRHYLNLDLMEKRLTNLLNDFGWGRMDQIFLE
                     KNR"
     gene            3713..4795
                     /locus_tag="PKZ49_04345"
     CDS             3713..4795
                     /locus_tag="PKZ49_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FemAB family PEP-CTERM system-associated
                     protein"
                     /protein_id="HND35825.1"
                     /translation="MSSIDTTLLESINKIAVTDSIKISLLNPDVASLWDDFVVSCKEA
                     TFFHRAGWKSVIENAFGHKTWFLYAERDQQIIGVLPLVEVNSHLFGHSLSSLPFCVYG
                     GIAAADDQARKLLDQAAQKLAIQLAVDYLEYRNITPYHSDWVTKGLYVTFRKEIDVDA
                     EKNMLAIPRKQRAMVRKGIKNNLQGSIDTDIDRFFYAYSTSVHRLGTPVFSKKYFRLL
                     KSTFGEDCELLIISSDHKVVSAVMSFYFRDEVLPYYGGGIDEARDLAANDFMYWELMR
                     RSCERGISIFDFGRSKLDTGSYSFKKNWGFEPQPLYYEYQLHKVKTMPDHNPLNPKYR
                     LFIQAWQRMPLALANFIGPHIVKNLG"
     gene            4805..6001
                     /locus_tag="PKZ49_04350"
     CDS             4805..6001
                     /locus_tag="PKZ49_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03087 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HND35826.1"
                     /translation="MQKLLYLTHRIPYPPNKGDKIRSYHVLQHLRKHFKVYLGTFIDD
                     EQDWQYVNHLNSLCEETCFVRLDPLLARMRSLIYLFSSMPLSLPYYRSSKLSTWVSNQ
                     LSSGTIKHTVVFSSAMAQYISETDIACSVIDFVDVDSDKWSQYASTKVWPQKWVYQRE
                     ADLLLEYEKYVTKKCSYATFVSEKEAGLFKQLSPEVENKITYFNNGVDTEYFSPSQNF
                     ANPYKPGTRVLVFTGAMDYWPNVDAVRWFAAELFPAIRTKIPDLEFYIVGARPTKTVL
                     ELSAIPGVVVTGSVDDVRPYLAFSEIAIAPLRIARGIQNKVLEAMAMEKLVVASPQAL
                     EGITAIPGIELFVADGAEAYIDHVISLLENPRMEAGKAARRRVIEDYNWDKNLSRIDR
                     LLQVSS"
     gene            6047..7624
                     /gene="xrtA"
                     /locus_tag="PKZ49_04355"
     CDS             6047..7624
                     /gene="xrtA"
                     /locus_tag="PKZ49_04355"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase A"
                     /protein_id="HND35827.1"
                     /translation="MNTMILPEASRTASLKELKESIYIVLMVIVAIFFIFYEATESIV
                     STWIRSETYAHGFLIFPFSLYMIWKKRTTLYGLAHYPEFKWLIVLGILGFGWLLAFLS
                     SVLVVKQFVLISMIPIVVCAIMGFRFAWAIAFPLAYLFFAVPVGESLIPPLIDFTADF
                     TVSALQMTGIPVYREGNFFSIPSGNWSVVEACSGLRYLIASVTLGTLYAYLTYISWVK
                     RLIFIIFSIIVPILANGLRAYLIVMTGHLSDMKLAVGVDHLIYGWVFFGFVMLLLFWM
                     GSLWREDDLEPQCEIQGSITVSSLEASASLKLKLLVACLVIASLIIWPIYGRHLDEQK
                     QEKETVDIRLVDRLGRWVPISQPISNWIPIYVGQPSKHYYHFKYNNQLVSVYITYYRN
                     QNEDSELINYANLLVEEMDPVWRQIEKSKYTLSVSSKKLEISQHQLTSHQSKLLIWRW
                     YWLINEQTANPYVAKANLAFNRILGYGDDGAEIILAAPYVNRPEEAAIVLQNFTNDMM
                     PAIMESLQLARANDSRH"
     gene            7647..8795
                     /locus_tag="PKZ49_04360"
     CDS             7647..8795
                     /locus_tag="PKZ49_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03088 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HND35828.1"
                     /translation="MNQSISPPLIAHVIYHLGVGGLENGLVNLINHLPQDRYRHVIIC
                     LKGYTDFSKRIKRSDVEVFDLNKPDGLNFSWYVSLYRLLRQLRPDIVHTRNFSTLEGQ
                     IVAALARINARVHGEHGRDILDLEGKNRKYNLLRQSIRPLIHRFITVSKDLERWLINT
                     IKVEPAHIKQIYNGVDNIRFSPKSKLNSITIPNNFIADNTFVIGSVGRMAKVKDYLSL
                     VRAFLLLVERIPQAKDHLRLLIVGDGESRKECIAMLRQADLENLAWLPGERSDVSELM
                     QLFDVFVLPSLAEGISNTILEAMSCGLPVIATSVGGNVELVIDSQTGKLVPSGNPEAL
                     ATAILEYYNHREMLIKHGQAARQLIETNFSMDTMVAGYLDVYDEIISR"
     gene            8857..10755
                     /locus_tag="PKZ49_04365"
     CDS             8857..10755
                     /locus_tag="PKZ49_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidotransferase 1, exosortase A
                     system-associated"
                     /protein_id="HND35829.1"
                     /translation="MCGIVGIFDTQGKREIDRRILNELNQTQTHRGPDEGELYLEPGI
                     GFGHRRLSIMDVSSGQQPLFNEDGSVVVIFNGEIYNYQELVKELVACGHQFRTHCDTE
                     VIVHGWEEWGEQCVDRFRGMFAFGVWDRNQDTLFVARDRLGIKPLYYALLKNGLFIFS
                     SELKAIHTHPEFVKEFDHFAIEEYFAYGYIPEPRTIYKDAQKLSPGYSIKLSPHSKQI
                     KPRQYWDIPFSPHSDMSEQAVEQELITRFREAVDIHLMSEVPLGAFLSGGVDSSAVVA
                     MMSGLMKDSVNTCSISFANPVFDESKYAQEVADRYHTKHYVDQVEQDDFDLIDKLAFL
                     YDEPFADSSAIPTYRVCELARKRVTVALSGDGGDENFAGYRRYRWHMYEEQLRSRLPI
                     GLRKPLFGLLGRLYPKADWAPKFLRAKATFEALARDSVEGYFHSVSIMDDNMRDQLFS
                     RDLKDTLNGYKAVEILRHYANKSPTRDPLSTIQYLDMKTYLVGDILTKVDRASMAHSL
                     EVRVPLLDHKLVEWISGLPASMKLSNNQGKYIFKKALEPYLPNDVLYRNKMGFSVPLA
                     EWFRKSLRNRVRESLLGKTLAETKLFNMDFIEKMIDQHQSGRRDFSAPLWTLLMFESF
                     LRSVHNKA"
     gene            complement(10789..12045)
                     /locus_tag="PKZ49_04370"
     CDS             complement(10789..12045)
                     /locus_tag="PKZ49_04370"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HND35830.1"
                     /translation="MAAITYQDFLNKASEVLQDPAHLNHKKALLLIDFEKLPALESAV
                     GFFPIDNILQQAFDLINHALHPSDIVGITGRHQICCLLTQLRSESHALLAAHKVLRIF
                     QQRFPLGERLIVLFPRIGIAFSDLCKNELGRLLGNAGSALQQARFEHEPIKVYEKKEE
                     DTILLGIDLWSDLGNAIEEGELTLYYQPQIDLATEKIISTEALIRWNHPQRGLIQPDK
                     FIKLAEGTELMGKLTLWVLNTALRHCASYRNAGIDAGVSINFSADNLLDPELPDLVLQ
                     GIEIWKIPPDKVMIELTESAIMEDRPGSLETLHELKKLGLKLAMDDFGTGFSSMERLL
                     NLPLDEIKIDMMFVKDMLTSPIHERIVDSMINLGHQLNLIVVAEGVEDAQTYERLKQL
                     GCDVIQGYFIGRPVPLTELIQQVTNK"
     gene            complement(12082..13308)
                     /locus_tag="PKZ49_04375"
     CDS             complement(12082..13308)
                     /locus_tag="PKZ49_04375"
                     /inference="COORDINATES: protein motif:HMM:NF023299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HND35831.1"
                     /translation="MKRIEIAVNQLKLGMFVSALDRPWLETPYLIEGVLIKSVEDITE
                     LARHCNYVYVDIEKSNPQSISNYAGQGTKNSSQADAATPFHGKAVYVDRCTAEKELPA
                     AQKAYDTTITLIDEIFECIERNTQLDIFAAQTAVESLRESVLRNADALLLLSRLKHSK
                     QALYDNALKVSVLLLAFGRHLGLPREELSELGLGGLLMDIGKLHLPPELLKKTTALTA
                     KEQEIMQKHVENGEAILARTPNIPEAVAKIVSQHHEREDGDGYPRKLTANQLHNFARM
                     AAIVDSYEDLTTARSGIKGLKPFQIIVELKNSARSGLNSVLVEQFSHCIGMFPVGSLV
                     ELNSREVAIILTHNRSQRFHPKVMVILDAKKQPYEKPFELDLKSTKPAPSGVPYEIVR
                     DLPLGAYNIDASQYYL"
     gene            complement(13434..14993)
                     /gene="guaA"
                     /locus_tag="PKZ49_04380"
     CDS             complement(13434..14993)
                     /gene="guaA"
                     /locus_tag="PKZ49_04380"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HND35832.1"
                     /translation="MHHKILILDFGSQYTQLIARRIRETNVYCELHPYDVSADFIKAF
                     SPIGIILSGGPASVFIEDTPRAPQIVFELGVPVLGICYGMQVMAAQLGGAVESAEKRE
                     FGYAELAIQDGPLFTGLQDRIDSSGKTLLDVWMSHGDSVTRLPENFKIIADNAATPIA
                     AMADEKRKFYGLQFHPEVTHTLQGKAILEHFAHDICAAGYDWNMPDYIEESVSRIRAK
                     VGNEKVILGLSGGVDSAVAAALIHRAIGDQLTCVFVDNGLLRLNEANQVMDVFSKHLY
                     AKVVHVDASQRFLSKLQGITDPEQKRKIIGKEFVEIFQHEAEKLVDAKWLAQGTIYPD
                     VIESADSKTKKAHTIKSHHNVGGLPETLHLKLLEPLRELFKDEVRELGIALGLPHDMV
                     YRHPFPGPGMGVRILGEVKYEFAELLRRADAIFIEELRISGWYERTSQAFAVFLPVKS
                     VGVMGDGRTYEYVIALRAVHTQDFMTAHWAELPYTLLGKISNRIINEVRGINRVVYDI
                     SGKPPATIEWE"
     gene            complement(15008..>15989)
                     /locus_tag="PKZ49_04385"
     CDS             complement(15008..>15989)
                     /locus_tag="PKZ49_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HND35833.1"
                     /translation="EGASREEALTLLHKHRLERVLIINDQYELKGLITVKDITKTTEH
                     PNASKDSQERLRVGAAVGVGEGSKERAEALIEAGVDVLIVDTAHGHSQSVLDQILWVK
                     KQFPSAQVIGGNVATATAAKALVDHGADAVKVGIGPGSICTTRIVAGVGVPQITAIEN
                     VANALQGTGVPAIADGGIRYSGDIAKAIAAGANTVMLGGLFAGTEESPGEIELYQGRS
                     YKSYRGMGSLSAMQQGSSDRYFQEHKQTEANKLVPEGVEGRVPYKGSIVAVIHQLVGG
                     VRSSMGYLGCKTIDEIHAKAEFIEITSAGIRESHVHDVQITKEAPNYHVE"
BASE COUNT         4611 a         3010 c         3507 g         4861 t
ORIGIN      
        1 gtgttgataa gctgactttg attgcagcag gaactcgtca tccccatgca acagaattac
       61 tggcaagtca gagtatggca gatattttaa tggagctagc acaacgttat agcgatcgta
      121 ttgtaatttt tgattctcct ccactccttc ttactagtga agcgcgcgtt ttagcaagtc
      181 aaatgggaca gattgttttg gtggtggaag cagaaagaac tactcaacag actgtcaagg
      241 aagcattgcg tcagattgaa tcctgtgatg ttattaattt aatctataac aaggccagaa
      301 cttcggcggc tgcaggttac tatggttatt attactcctc atgaagctgc tttctaaaca
      361 tttctgcaat tatcattaca agggcttagt ttacattagt attttgctat ttaatattat
      421 agctttacct atatacgcag gagaatggcg cgttgtacct agtttgaata ttaatgaacg
      481 ctatacggat aatttaagac tgggtggtat agcgacagga ggtaaaggga atgatgaatt
      541 tatcactcag attaatccaa gcatcctcat taatggaaat gcccgacgtt accgagtaga
      601 cgttaattac ttaatgaata atctgatata tgcgaataat agtgaattta acagaattag
      661 gcatcagctt aatgctaatg gaactattga gttaattcag gatcggtttt tcattgatgg
      721 tcgggccgtg attttacagc aaaatgccag tctgcttggt tctcagggat tagataatgc
      781 taacccactt aatcgaagaa atatgagtat gttaaacata agcccatact atagacatcg
      841 ctttggtgat tttgcaactg ctgagttacg ttatacccat ggagaagtga atacgagtgg
      901 ggggggattg cgagactctt caacagacaa tggcacagtt cgtttgaaca gtagtacggc
      961 tttcagggat ctgcaatggg gtattaccta caatcatact gagattcatc ggagtcaact
     1021 taggacgatt gcgcttgagc gatctacagt gctgctaaga tatatgctaa cgccacaatt
     1081 tagtcttgta ggtacgggag ggtatgaaag aaatagcttt atctccgtta atggaggcac
     1141 gagtagccct acctggacag tgggtttttc ctggataccc aatgaaagaa cggaatttga
     1201 ttttgctgga ggtcaaagga tttttggtga tacgtattta gctaatttta gtcatcgcac
     1261 tcgattaact acttggagtg catcttataa cgaagatata accacctttg gacaacaaaa
     1321 tttattgcaa cctggagcgg caggtgctat tccttctgga gggatattgc ctcagcttct
     1381 attaaataca ctgacgaatg ctggctttgg ggggggggga cttgcaaatc aaggtgcctt
     1441 attgggtatc tcgggtgcta ataacttttt taccaatcgg ttatttcttc aacggagctt
     1501 acaagcgttt atgaccatca atggaagtcg taatacggta gtttttagag ggttcaacat
     1561 atcgcgacag gcttatactg gagatgaaga agatgattcc attggaggaa gagtcaattc
     1621 cgtactaaat aggaatacgg ttcaaacagg gggaggggtt tcttggattt ataaattgtc
     1681 gccgctcatg aatttcacaa ctaactacac ttttactcga ataaatttta cgacaactaa
     1741 tctaactgat attaaccaaa taatgatggt tagtttgaat aaaagatttt ctcctaatct
     1801 atttggcgaa attagctatt ttcataatcg aagagactca gaacggatca atggggattt
     1861 ttcttcgaac aactatatgg catctctttc aatgaatttc taaatgtttg tttcaactaa
     1921 tttaatttat gtacctatct ttctatggtt taaaaacaaa accgtttcaa ttaaagcctg
     1981 acgctagttt cttttttagt agtaaaggcc ataagcgcgc gattgcttat ttagaatatg
     2041 ggatatcaca ggaagatggt tttatcatca ttaccggtga agttggtgca ggaaaaacca
     2101 cactcatccg ccatctcttc cagaaattgg atcagcagcg ttttattgtg gcgcaactag
     2161 ctaacacaaa tctcgattca gatgacatgt tgagaatggt tgcagcttct tttggattac
     2221 cagtgaccaa tacatcaaaa gcaacgacgc ttttagaaat cgagcagctt cttaggcaat
     2281 atgataagga agggaaaaaa atattattgt tagttgatga ggcgcagaat ttaacatcgc
     2341 gagcacttga agaattaaga atgctttcta attttcagac agacgataag cttttattac
     2401 agacctttct attaggtcaa ccagaattta gaaaaacatt attaggtaat aacatgcagc
     2461 aactacgcca gagagtgatc gctacttatc atcttggccc aatggataat actgaaacac
     2521 gcgcttatat tgagcatcgt ttggcgattg caggctggca gggaagtccg ttactgcagg
     2581 atgatagctt tgatgtgatt tataacttca caggtggtat tccacgaaaa attaatacgc
     2641 tatgtgatcg attgctaata atgggatgct tagaggaact acgcacgata acaagtcaag
     2701 aagtgaatga ggttgttgcc gacattcaac aagagtttga tttaccgcct tttcaaaata
     2761 ttgaaacaag tgattaattt agtattcaat ggcgccctga aaaagggttt gttgcatcaa
     2821 aaatcgatta ggtttgctta gcttttgatt gttgtacacg atagggaaag atcaatatga
     2881 tacgtaatgc tttaacgatt gatgttgagg attatttcca agtatcagcg tttgatccct
     2941 atattccgag aaatgattgg gatagtattc cttgtcgtgt ggagcggaat attgatcgga
     3001 ttctttcatt gctcgaaaaa aaaggcataa atgcgacttt ttttactttg ggttggatag
     3061 ctaaacgcta tccctcgatg gttcggcgta ttgtagagaa tggtcatgag ttagcaagcc
     3121 atgggtgtga tcatgatcgg gttacagagc taggatcaga tgagttttat aaagatatcg
     3181 ccgacagtaa aaaaatactt gaagatataa gtggattccc agttattggt tatcgagccc
     3241 caagtttctc gattaataag cagaattatt gggcattaaa cttacttgaa gaagcaggtt
     3301 atctctatag ttcaagtatc tatccagtac gtcacgatca ttatggtatt cctgatgctc
     3361 cccgctttgc tttttatccc aacccgacag ggaagttact cgaagtaccc attacaacag
     3421 taaatttatt ggggcgtaat attccagtgg gaggaggtgg gtactttcgt ttgtggccgt
     3481 acgctgtttc acgctggttt attcaaaggg taaatgagaa agatcagcgt tctgctgtat
     3541 tctattttca tccttgggaa atagatcctg atcagcctcg ccaaaaaggt ataaatctta
     3601 aagtgcgctt ccggcactac ttaaatctag atttaatgga gaaaagactg actaacttgc
     3661 tcaatgattt tggttgggga agaatggatc aaattttctt ggaaaaaaat agatgagttc
     3721 tatcgatacg actttacttg agtctataaa taaaattgcg gtaacagatt ctataaagat
     3781 atctttgttg aaccctgacg ttgcttcact atgggatgat tttgtggtct cctgtaaaga
     3841 agcgactttc tttcatcgag cgggttggaa aagtgtaata gagaatgcat ttggtcacaa
     3901 aacatggttt ctctatgctg aacgtgatca acaaattata ggtgtattac cattagtaga
     3961 agtgaacagt cacctctttg gccactcact gagttcgctc cctttttgtg tatatggtgg
     4021 tattgctgcg gcagacgatc aggcaagaaa gctgcttgat caagcagctc agaagttggc
     4081 cattcaatta gcagttgact atctcgaata tcgcaatatt acaccttacc attctgattg
     4141 ggtaaccaaa gggctctatg taacatttag aaaagaaatc gatgtagatg ctgagaaaaa
     4201 tatgttggct atacctcgga agcaacgcgc aatggttaga aagggtatta agaataatct
     4261 gcaaggtagt attgacactg atattgatcg gttcttttat gcttattcga ctagcgttca
     4321 tcggttaggt acgcctgttt tctcaaaaaa atattttcga ttgcttaaat ctacttttgg
     4381 agaggattgc gagttattga tcatttcatc agatcataaa gtagtaagcg cagtcatgag
     4441 tttttatttc cgagatgaag tgcttcccta ttatggaggg gggatagatg aagcgcgtga
     4501 tttggcagca aatgatttta tgtactggga attaatgcgt cgctcttgtg aaagaggaat
     4561 cagcatattt gattttggaa gaagcaagct tgatacagga tcctatagtt tcaagaaaaa
     4621 ctggggtttt gaacctcaac cactttacta cgaatatcag ctacataaag tcaaaacaat
     4681 gccagaccat aacccactta atcctaagta tcgcctcttt attcaagcct ggcaaagaat
     4741 gcctttggca cttgccaatt ttattgggcc acatatagta aaaaatctag gctagagaaa
     4801 agcaatgcaa aaattgcttt atcttacaca ccgtatacca taccccccca ataaagggga
     4861 taagattcgt tcatatcatg tattacaaca cttaagaaag cattttaaag tttatctagg
     4921 tacatttatt gatgatgaac aggattggca gtatgtgaat catctgaatt cattatgtga
     4981 agaaacttgc tttgtcaggc ttgatccgct attggcgaga atgcgtagtt tgatttactt
     5041 gttttcgagt atgccattat cgctgcctta ttatcgaagt tccaagctga gtacctgggt
     5101 tagcaatcaa ttgtcaagcg gaacgatcaa acatacagtc gttttctctt ctgcgatggc
     5161 tcagtacatt agcgaaacag acatcgcgtg tagcgtaatt gattttgtag atgttgattc
     5221 tgataaatgg agtcaatatg cgtcgactaa agtgtggcct cagaaatggg tttatcagcg
     5281 tgaggctgac ttgctccttg aatatgaaaa atatgtcact aagaaatgta gttacgctac
     5341 ctttgtttca gagaaagagg ccggtttatt taagcagtta tctccggaag tagagaataa
     5401 aattacttac ttcaataacg gcgttgatac cgagtatttc tcaccgagtc aaaattttgc
     5461 taatccctat aaaccaggca ctagagtctt ggtgtttact ggagcaatgg actattggcc
     5521 taatgtcgat gcggtaagat ggtttgcagc tgaactattt ccagcgatac gtacaaagat
     5581 acctgatttg gagttttaca tcgtcggtgc tagacctaca aaaacggtat tggaattatc
     5641 cgctattcca ggagtagtgg tgacggggag tgttgatgat gtaagacctt atttagcatt
     5701 ctcggaaatt gcaattgcgc cacttcggat agcgcgaggg attcaaaaca aagtgcttga
     5761 ggcgatggcg atggaaaaat tagttgttgc ttcacctcaa gcgctggaag gtattactgc
     5821 cattcccgga attgaactat ttgtagcaga tggtgctgaa gcatatatcg atcacgtgat
     5881 cagcttatta gagaaccccc gaatggaagc agggaaagca gcgcgtcgca gagtaataga
     5941 ggattataac tgggataaga atctatctag aatagatcgc ttacttcagg tatcgagtta
     6001 acacaaaatt cgattcttgc catagtggct aacaattgat tacgaaatga atacaatgat
     6061 tttacctgag gcgtcgagaa cggcatcttt aaaagaatta aaagaatcga tttatatcgt
     6121 gttgatggtt atcgtcgcca tattctttat cttttatgaa gcgactgagt caattgtatc
     6181 aacctggatt cgatctgaaa cttatgcaca cggcttctta atttttccat ttagtctgta
     6241 tatgatttgg aagaagcgaa caacacttta tggtctagct cattatcctg agtttaaatg
     6301 gctaatcgtt ttgggtattt taggctttgg gtggcttctt gcttttcttt ctagcgtact
     6361 tgttgttaag caatttgttt tgatctccat gatacccatt gtagtttgtg caatcatggg
     6421 tttcagattt gcatgggcta tagcatttcc ccttgcttat cttttctttg ccgttcctgt
     6481 tggggaatcc ttaataccac cactaatcga ttttactgct gactttacag tgagtgcgtt
     6541 gcaaatgact ggaatccctg tctatcggga aggtaatttt tttagtatcc caagtgggaa
     6601 ctggtctgta gtggaggctt gtagtgggtt acgctatctc attgcgtccg ttactttggg
     6661 aacgctctac gcttatttga cttatatcag ttgggtaaaa cgattaattt ttattatctt
     6721 ttctattatt gtacccattc tagccaatgg actccgagct tacttgatcg ttatgacagg
     6781 gcatttgagt gatatgaagt tggcggtagg ggtagatcat cttatatatg gttgggtttt
     6841 ctttggtttc gttatgttat tgttgttttg gatgggttct ctttggcggg aagacgatct
     6901 ggagcctcaa tgcgaaattc agggatcgat aactgtgagt tcattagagg ccagcgcttc
     6961 actaaagtta aagttacttg tagcttgttt agtcatcgct tcgctaataa tttggcctat
     7021 ttatggcagg cacttagacg aacagaaaca agaaaaagaa actgttgata ttcggctcgt
     7081 agatcgacta ggaagatggg ttccaatttc gcagccaata tcaaattgga ttccaatcta
     7141 tgttggtcaa ccaagtaagc attattacca ttttaaatat aataatcagc tggttagtgt
     7201 gtatatcaca tattatcgta atcagaatga agattcggaa cttattaatt acgctaatct
     7261 actggtggaa gaaatggatc cagtttggcg acaaattgaa aaatcaaaat atacgctatc
     7321 agttagttct aaaaaattag aaataagcca acatcaactg acttcccatc aatctaaact
     7381 gttaatttgg agatggtact ggttgattaa tgagcaaaca gcaaaccctt atgttgctaa
     7441 ggctaatctc gcttttaata gaatattagg ttatggcgat gacggggctg aaattatttt
     7501 agctgcccct tatgttaatc gacctgaaga ggcagcgata gtattacaaa attttacgaa
     7561 tgatatgatg ccagcgatca tggaatcact tcaattagca cgagctaacg atagtagaca
     7621 ctaacaacat ctttctaatc aatatgatga atcagtctat atctcccccg cttattgcgc
     7681 atgttattta tcatttaggt gtaggtgggc tagaaaatgg tttggttaat ctcattaatc
     7741 accttcctca agatcgttac cgccatgtca tcatttgcct caagggctat actgattttt
     7801 ctaaacgaat taaaagaagc gatgtggaag tttttgatct taacaagcca gatgggttaa
     7861 atttttcctg gtatgttagc ttatatcgtt tactgaggca gttgagaccc gatatcgtac
     7921 acacacgtaa tttttctaca ttggaagggc agatcgttgc ggccttggcg agaatcaatg
     7981 cacgtgttca tggagagcat ggcagagata tcttagatct tgaaggaaaa aatcgtaaat
     8041 ataatttact acgacaatct attcgtcctt taatccaccg ctttattacg gtaagtaaag
     8101 atttggaaag atggcttatc aatacgataa aagtcgagcc agctcatatc aagcaaattt
     8161 ataatggtgt tgataacata cgattcagcc ctaaatccaa gttaaattca ataacgatac
     8221 ccaataattt cattgctgat aacacatttg tcattggaag tgtagggaga atggctaaag
     8281 tcaaagatta tcttagtttg gttcgagctt tcttactact agtagaacga attcctcaag
     8341 caaaagatca tctaagactt ttaatcgtcg gagatggcga atcacggaaa gaatgtatag
     8401 cgatgttgcg tcaggcagat ttagaaaatc tggcctggtt accaggagaa cgttctgatg
     8461 tatcagagct aatgcaatta tttgacgttt ttgttttgcc atcccttgcc gagggaattt
     8521 caaatacaat tttagaagca atgtcatgtg gattgcccgt tatagctaca agtgttggtg
     8581 gcaatgtgga gcttgtaatc gattcacaaa ctggaaaatt agtaccatcg ggtaacccag
     8641 aagcgcttgc tacagcgata ttggaatatt ataaccatcg ggaaatgctc ataaagcatg
     8701 gtcaggcggc tcgtcaatta atcgaaacta atttcagtat ggatactatg gttgcaggct
     8761 atcttgatgt ctacgatgaa attatcagtc gataattagc atctatcaat taacaaaatt
     8821 ggaaaaatta taaattaatc gggagaggtt gctcgtatgt gcggaattgt aggtattttt
     8881 gacacgcaag gtaaacgcga aatcgatcgt cggatactaa acgagctaaa tcaaacgcaa
     8941 acccatcgtg ggccagatga aggagagctc tatttggagc ccggtatcgg atttggccat
     9001 cggcgattat caattatgga tgtatcaagt ggccaacagc cgttgtttaa tgaagacgga
     9061 agtgttgttg tgatttttaa tggtgagatc tataactatc aggagttagt aaaagagcta
     9121 gtcgcctgtg gtcaccagtt tcgtacccat tgcgataccg aagtgatcgt gcatggatgg
     9181 gaggaatggg gtgaacagtg cgtagataga ttccgtggta tgtttgcttt cggcgtctgg
     9241 gaccgtaatc aagatacttt gtttgttgct cgagatcgac tgggaattaa accgctgtat
     9301 tacgcattat tgaaaaatgg tctattcatt ttctcatcgg aactcaaggc gattcatact
     9361 cacccagaat ttgttaaaga attcgatcat tttgcgattg aagaatattt tgcctatggc
     9421 tatattccag agcccagaac gatatataaa gatgcccaaa agctctctcc tggctattcg
     9481 ataaaattat caccccatag caaacagata aaaccgcgtc aatactggga tataccgttt
     9541 tcacctcatt ctgatatgag cgaacaggca gtcgaacaag aattgattac tcgtttccgc
     9601 gaggcagtgg atattcattt aatgagtgaa gttccgctgg gtgcattttt atctggaggt
     9661 gttgattcca gtgcagttgt tgcaatgatg agcggtttga tgaaggattc tgttaatacg
     9721 tgctcgattt catttgctaa tcctgtcttt gatgagtcta aatatgcgca agaagtagca
     9781 gatcgttacc atacaaaaca ttatgtagat caagttgaac aggacgattt tgatctaatt
     9841 gataaactcg ctttcttata tgatgagcct tttgctgata gctctgcgat tccaacctat
     9901 cgtgtgtgcg agcttgcaag gaaacgagta accgttgctt tatcagggga tggtggtgat
     9961 gaaaattttg caggttatcg tcgctatcga tggcatatgt acgaagagca attgagatcg
    10021 agactaccca ttgggttacg aaaaccatta tttggcttgt tagggaggct ctatccaaaa
    10081 gcagattggg cacccaagtt tcttcgtgcc aaggcaacct ttgaagcgct tgcacgcgat
    10141 tcggtcgagg gttatttcca tagtgtttcg attatggacg ataatatgcg tgatcagctc
    10201 tttagtcgcg atttaaaaga tactttgaat ggctataaag ctgttgaaat tttacgtcat
    10261 tatgcgaata aaagccccac tcgtgatcca ttatcgacta ttcagtattt ggatatgaag
    10321 acttatcttg taggggatat cttaacgaaa gtcgatcgag ccagtatggc gcactcatta
    10381 gaggtaagag tacccttgtt agaccataaa ttggttgaat ggatatcggg attacctgct
    10441 tcgatgaaat tatctaataa tcaagggaaa tatattttta agaaagcact tgagccttat
    10501 cttcccaatg atgtacttta tcgcaataaa atgggttttt ccgttccctt agcggagtgg
    10561 tttagaaaaa gcttacgtaa ccgagtgagg gaatcattac tgggtaaaac attagcagaa
    10621 accaaactgt ttaatatgga ttttattgag aaaatgattg atcagcatca atcgggaaga
    10681 agggatttta gcgcaccttt atggacatta ttaatgtttg aatccttttt gcggagcgtg
    10741 cacaacaagg cataaatgct attttgtctt ggtaatggtg gctagatttt atttgttcgt
    10801 tacttgttga ataagctcag tcaggggaac tgggcgtcca ataaaatatc cttgaatcac
    10861 atcacaaccc agctgcttta ggcgttcata agtttgagca tcttccacac cttctgccac
    10921 aacgattaaa ttgagttggt gtcccaagtt aatcatcgaa tctacaatcc gctcgtgaat
    10981 aggcgaagtt agcatatctt ttacgaacat catgtcgatc ttgatttcat caaggggtaa
    11041 attcaataag cgttccattg aagaaaaacc agtaccaaaa tcatccatgg ccaattttaa
    11101 tcctagtttt ttaagttcat gcaacgtttc cagtgagccc ggccgatctt ccatgatggc
    11161 tgattcagtt aattcaatca tgactttgtc gggtggaatt ttccatattt caatcccttg
    11221 caagactaga tcaggtaatt cgggatcaag caggttatca gcagagaaat taatggaaac
    11281 acccgcatca atgcccgcat ttctgtagga agcgcaatgc ctaagcgcgg tattaagcac
    11341 ccagagtgtg agcttaccca tcagttctgt tccctcggca agttttataa atttgtcagg
    11401 ttgtattaac cctctctgag gatgattcca gcggatgagc gcttctgttg atatgatttt
    11461 ttcagtagct aaatcaatct gcggttgata gtataaagta agctctcctt cttcgatggc
    11521 atttccaagg tctgaccaaa gatcaatacc aagaagaatc gtatcttctt cctttttctc
    11581 ataaacctta ataggttcat gttcaaatct ggcttgctga agtgcagatc ctgcattacc
    11641 aagaagtcgg cctaattcat ttttacacaa atcgctgaat gcgatgccta tccgtggaaa
    11701 tagcacaata agccgttcac ctaatgggaa acgttgctga aaaattcgta gcactttatg
    11761 tgcggctaac aatgcatgac tctcagagcg cagctgagtc aataaacagc aaatttgatg
    11821 gcgaccagtg attccaacaa tatcgctggg atggagcgca tgattgatga gatcaaacgc
    11881 ttgttgcaga atattatcaa tcggaaaaaa accgactgca ctttcgagcg caggtaattt
    11941 ttcgaagtca atcagtagca aagctttttt atgattgaga tgagctggat cctgtagtac
    12001 ttcagatgct ttattcagaa aatcttggta agtgatggca gccatgggga aaataaataa
    12061 gtgtatttat caacccattt actatagata atattggcta gcatcaatat tataggcgcc
    12121 taatggtaaa tctcttacga tctcataggg aaccccgctt ggtgcaggct tagttgattt
    12181 taagtcgagc tcaaaaggtt tttcataagg ctgtttctta gcatctagta taaccattac
    12241 tttgggatga aatcgctgag agcgattatg cgtcaaaatg atcgcaactt cgcgagaatt
    12301 gagctcaacg agtgagccga caggaaacat tccgatgcaa tgggagaatt gttctacgag
    12361 tacagaatta agcccgctgc gtgcgctatt tttaagctca acgatgatct ggaaaggttt
    12421 taacccttta atacctgacc gagcagtcgt gagatcctca taactatcta caatcgcagc
    12481 catgcgtgca aaattatgaa gttgattggc agttagcttt cttggataac catcgccatc
    12541 ttcacgttcg tggtgctgcg acactatttt tgcaactgct tcaggaatat taggcgttct
    12601 agccaggata gcttcgccgt tttcaacatg cttttgcatg atttcttgtt ctttggcggt
    12661 aagtgcggta gtttttttga gaagttctgg aggaaggtga agtttaccaa tatccatgag
    12721 caaaccaccg aggccaagct cagataattc ttctcttggg aggccaaggt ggcggccaaa
    12781 agcgagtaat aaaacagaga ctttaagggc gttatcatac aacgcttgtt ttgaatgctt
    12841 aaggcgcgaa agtaggagta atgcgtcggc atttcgtaaa acactttctc gcagcgattc
    12901 tacagcagtt tgagctgcaa aaatatcaag ctgagtattg cgttcgatac attcaaaaat
    12961 ttcatcgatg agtgtaattg tagtgtcata cgctttttgt gctgcgggta gttctttttc
    13021 agcagtgcat cgatcaacat aaaccgcttt accatgaaag ggggttgcag catccgcttg
    13081 actgctattc tttgtacctt gacccgcata atttgaaatg gattggggat tgcttttttc
    13141 gatatcaacg tagacgtagt tgcagtgacg agctaactca gtgatgtctt cgacagattt
    13201 gatcaaaacg ccctcaatta gataaggcgt ctcaagccag ggcctatcta aggcactaac
    13261 aaacatacct aatttcaatt gatttactgc tatctctatc cgcttcatca gggcaatttt
    13321 agagggtttt aacttagcag aatcttggaa aagagttact tttttgaggt ttatgctgtt
    13381 taagcgcttg attcggaaaa gaacagagtg aatgaaatta agtagctatg atttcattcc
    13441 cactcaattg tcgcaggcgg tttaccggag atatcataaa cgacgcgatt aataccacga
    13501 acttcgttga tgattcggtt agagatttta ccgagcaacg tatagggaag ttcagcccaa
    13561 tgagctgtca tgaagtcttg tgtatgcacg gcacgaagtg caattacata ttcataggta
    13621 cggccatcgc ccatgacacc gacagactta accggtaaaa aaacagcaaa agcttgagat
    13681 gttctttcat accatcccga aattctaagc tcttcgataa agattgcatc agcgcgcctt
    13741 agcaattctg caaattcgta tttgacctcg ccgagaattc tgacccccat tccaggcccc
    13801 ggaaaagggt ggcgataaac catatcatga ggaagtccta acgcgatacc taactcgcgt
    13861 acttcgtctt taaataattc acgtaaaggc tcaagcagct ttaaatgaag tgtttcaggt
    13921 aatccaccca cattatgatg ggacttaatg gtgtgtgctt tttttgtttt gctatcagca
    13981 gattcaatca catcagggta gatcgtgcct tgtgctagcc attttgcgtc aaccagcttt
    14041 tcagcttcgt gttgaaaaat ctcaacaaat tccttcccaa tgattttacg tttctgttct
    14101 gggtcggtaa taccttgaag ttttgagaga aatcgctggc tcgcatcgac atgcactact
    14161 ttggcgtaaa ggtgtttgga aaaaacatcc atcacttgat ttgcttcatt taggcggagt
    14221 aacccgttat caacaaaaac gcaggtgagt tgatcaccaa tcgcccggtg gatgagtgca
    14281 gcggcgacag cagagtcaac accacccgat aagccgagaa tgactttctc gttaccgact
    14341 ttggcgcgga tgcgactgac tgattcctcg atgtagtctg gcatattcca atcatagcca
    14401 gctgcgcaga tatcatgcgc gaagtgttcg agaatagctt ttccttgcag ggtatgggtt
    14461 acttcaggat gaaactgtag cccataaaat tttctctttt catctgccat tgctgcgata
    14521 ggtgtggcag cattatcggc aatgatttta aaattttcgg gtaagcgcgt gacgctgtcg
    14581 ccatgactca tccaaacatc cagtagtgtt tttccgcttg aatcaattcg atcttgtaac
    14641 ccggtaaaaa gtgggccatc ttgaatagcg agttctgcat agccaaactc acgtttttca
    14701 gccgattcga cagcaccgcc aagttgtgcc gccatgactt gcattccata acagatgccg
    14761 agtacgggaa cgccgagttc gaacacgatt tgaggggcac ggggagtatc ttcgataaaa
    14821 acggatgcag gcccgccgga tagaataatg ccaataggag agaacgcttt aataaaatca
    14881 gcgctaacat cataaggatg taattcgcaa tacacattgg tttcccggat acgacgggca
    14941 atcaactggg tgtactgaga cccaaaatcc aagatgagga ttttatggtg catagatgac
    15001 ttgcttgtta ttcgacgtga taattaggag cttctttggt gatttgtaca tcgtgcacat
    15061 gtgattcacg aattcctgct gaagtgattt cgataaactc agctttagca tgaatttcat
    15121 caatggtttt acaaccgagg taacccatgc ttgagcgaac acctcctacc aattgatgaa
    15181 tgactgccac aatcgatcct ttatagggga ctcttccctc aacgccttct ggaactagct
    15241 tgtttgcctc agtttgtttg tgttcttgga aatagcgatc gcttgatcct tgttgcatag
    15301 cagaaagtga tcccatgcct cgatagcttt tgtaagaccg gccttgatat agttcaattt
    15361 cgccaggcga ttcttcagtg ccagcaaaaa gaccaccaag cataacagtg tttgcccctg
    15421 cagcaatggc tttggcaata tcgcctgagt agcgaatgcc accatctgca atggctggta
    15481 cgccagtacc ctgaagggca ttagcaacat tttcaatggc tgtgatttgt gggacaccca
    15541 cgcctgcaac aatgcgagta gtacaaatcg acccaggccc aataccgact ttgactgcat
    15601 cggcaccatg atcgactagt gctttggctg ctgttgctgt cgcaacatta ccgccgatga
    15661 cttgggcaga tggaaattgt tttttgaccc aaaggatctg atcgagcaca ctctgagaat
    15721 ggccgtgggc cgtatcgaca atcagtacat cgacaccggc ttcgatgagc gcttcagcgc
    15781 gttctttact accttctccc accccaactg cagcaccgac tcgcaaccgt tcttgactat
    15841 ctttgctggc attgggatgt tcggttgttt tagtaatgtc ttttacggtg ataagaccct
    15901 tcaattcata ctgatcattg ataattaaca ctctttccaa gcgatgttta tgtaaaagcg
    15961 taagcgcttc ttctcttgaa gcgccttcc
//
LOCUS       DAOQKQ010000044        15948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_1689_length_15948_cov_4.759517, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000044 DAOQKQ010000000
VERSION     DAOQKQ010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 15948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15948
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1689_length_15948_cov_4.759517"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="PKZ49_04390"
     CDS             <1..444
                     /locus_tag="PKZ49_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HND35834.1"
                     /translation="KVENGTQLVDQAGRTMDEVVMSVKRVTDIMAEISAASQEQSAGI
                     EQVNLAISQMDEITQQNAALVEQAAAASESMHEQAAQLAQAVAVFKLQHAAQAAKHQG
                     EKKIDRRGPDRPGNVQRLPKTANQASKKETMPVAKTGTDGDWEEF"
     gene            492..3323
                     /locus_tag="PKZ49_04395"
     CDS             492..3323
                     /locus_tag="PKZ49_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HND35835.1"
                     /translation="MRLNLPVNDVEREMRDGEFLVSKTNLKGVITYVNQPFIEMSGYT
                     KEELIGQAHNLIRHPDMPPAAFEDFWKTLKQGRPWSGFVKNRAKDGSFYWVFANATPI
                     NEAGQITGYLSVRSKPTREQIRAAENLYREMREGRAKVKINAGDVVPLTLIGRLKQRL
                     RFVSVRTRIIMMLSFFSVIIICLGITGLLGFNQSNEALRSMHIDRTGALEDLSMILDK
                     IQQIQIHSHVVSSQNNLEVVKKRYAMVTEREKELDERWTEYLGTYLLPLEKELAETFH
                     SQMKAFREASAITLNAALNGDYERAYRNQINTVEPKFELAYTTLVKLIEFQAAAAKND
                     YELAVEKFTLGRNYGLFVIALSIFVSILGGYALLLAITRPLARLKRIAEKISGGNFTS
                     QITIANYDEIGSLTETIKTLQICSGSSLDEAQRTAAENFRIRTVLDQSTAAVMIADSA
                     NTIIYMNQTMRKALIAYQAEFQKVIQNFKPDAIVGNDFSYFQQSINLLGLTKPTKQTI
                     NIGERIYLLTLVPVLDGSGNRMGTSIEWLDRTIEVKVEQEIASVVGAAGEGDFSKRLS
                     LEGKEGFFAQLARNINNLVETSASGLSEVVRVLGALSKGDLTEKITNDYKGMFAKLKA
                     DSNATIEQLKHIIHKIKESADSISTASQEIAQGNMDLSQRTEAQASNLEETASSMEEL
                     TSTVKQNAENAKQANQLAASASDVAVKGGEVVGQVVETMSSINESSKKIVDIISVIDG
                     IAFQTNILALNAAVEAARAGEQGRGFAVVATEVRTLAQRSASAAKEIKDLIGDSVNKV
                     ENGTQLVDQAGRTMDEIVMSVKRVTDIMAEISAASQEQSAGIEQVNLAISQMDEITQQ
                     NAALVEQAAAASESMHEQAAQLTQAVAIFKLHHAAGTAVHCNEEKMERRGSDRPTNVQ
                     RLPKAANQVPEKEMIPVAKTGTDGDWEEF"
     gene            3377..6187
                     /locus_tag="PKZ49_04400"
     CDS             3377..6187
                     /locus_tag="PKZ49_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HND35836.1"
                     /translation="MRINMPINNVERQVNENDYLVSKTNTKGVITYVNQPFIEISGYT
                     KEELIGQAHNLIRHPDMPPAAFEDFWKTIQQGRPWSGVVKNRAKDGSFYWVYANVAPI
                     YEAERITGYMSVRSKPAREQIQQAELLYREMREGRAKFKIEAGELIPANWFGHIKQKM
                     RSFTIKSRINVISCLAIVSLSFMGGWSLLGDYQSKERLRDVYENRTLVLIDLSTILDA
                     IQRMRVNALTAANSNNPVIAKERFADTNQLDRIFDENWSRYSVTSVTTEEKVKAEEFY
                     RNVEAYRISRDKTMNLSIEGDFEAARENAVKYARPKYDLVHAALVDLFEIQEKQTKVV
                     FDTAIEQFEILKISTIAMILFFTFIYLYLTRLLHRAINLPLASVSAIFRKIAAGDYTN
                     QVAIVNHDEIGCLTESVQMMQTRAGAEIDEIKRVAAENRRIRNALDQSNASIMIVDPN
                     SCIIYVNQAMQILLKNNEKEFVAVLAGVQINAILGQSFTIFNEKLDLSEINKSTKQLL
                     KIGSSIFVLTLTPVFDDNGSRLGTSIEWLDRTAEAAVEDEVAQIVKSASEGDFSKRLE
                     TKSKNGFFEQLAKNINQLLENNEKGLTDIARMLGAMAKGDLTQTITRQYKGMFEKLKT
                     DANATVQQLTDIIRQIKESADSINITSHEIAQGNTDLSQRTEQQSISLDNTASSMEEL
                     TATVKQNAENAKQANQLAVSASDVAIRGGEVVGQVVETMSSINESSKKIVDIIGVIDG
                     IAFQTNILALNAAVEAARAGEQGRGFAVVATEVRTLAQRSAAAAKEIKSLIGDSVNKV
                     ENGTQLVDQAGRTMDEIVMSVKRVTDIMAEISAASQEQSEGIEKVNATITQMDEITQQ
                     NTALVEEGAAASESMYELANELTKLVAVFRTNSSISLIAEAETESGQIERRGPHRATN
                     VQRLPLSAKLVTNSIEDTVAVT"
     gene            6264..7121
                     /locus_tag="PKZ49_04405"
     CDS             6264..7121
                     /locus_tag="PKZ49_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HND35837.1"
                     /translation="MNLDPVIDQPMREYDFTNQDFACVKRLIYQRAGISLAPSRSNMV
                     YTRLVKRLRETGYSQFKDYLAMLEENSDSGEWESFVNALTTNLTSFFREAHHFEMLTE
                     HLKRRKPGQTIKIWCSASSTGEEPYSLAMTAVETFDTYTPPVRILATDIDTNVLATAQ
                     AGIYSMNQLENFSDARLRRFFLKGKGSNAAFARIRPELKNLVAFKQLNLLDRSWPLKG
                     PFDAIFCRNVMIYFDKPTQHQLLDKFAPLLATDGLLFVGHSESFQYNENPFKLLGKTV
                     YELINKHSR"
     gene            7118..7708
                     /gene="cheD"
                     /locus_tag="PKZ49_04410"
     CDS             7118..7708
                     /gene="cheD"
                     /locus_tag="PKZ49_04410"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemoreceptor glutamine deamidase CheD"
                     /protein_id="HND35838.1"
                     /translation="MTTIDSDHLATTAYYDPIHQIDAVKLLPGEYYVTQKDILLVTVL
                     GSCVAACIWDSLLRIGGMNHFMLPGDENSGSSARYGDFAMSLLIEQLLKLGARKENLK
                     AKVFGGGNVLPGFAVTSIGERNAEFTLDYLQHQGIPIIAHDLTDVFPRKIYFFPRTGR
                     ALVKKLHVKNNTIFERERSHLAEIKQGFALTRSVMS"
     gene            7770..8852
                     /locus_tag="PKZ49_04415"
     CDS             7770..8852
                     /locus_tag="PKZ49_04415"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HND35839.1"
                     /translation="MKKKISVLIIDDSALVRNLLSSIINSQEDMEVIGAAPDPLIARE
                     MIRRLNPDVLTLDIEMPKMDGLEFLEKLMRLRPMPVIMISTLTEKSAEATMEALALGA
                     VDFVSKPKVDVASELQLYREEIARKIRMAVSVQIKKPTPVSTDTNQTASSAFGANRIY
                     STEKLIIIGSSTGGTEALKDFLVNLPPHTPGILIAQHMPEAFTKSFATRLDSLCKISV
                     KEAQGGERVLPGHAFIAPGHSHLLLKRSGANYVTELSQSEPVNRHRPSVDVLFRSAAT
                     YAGKNAIGVILTGMGRDGAAGMLEMRKAGAYNFAQDEASCVVFGMPKAAIEVGATDEV
                     VPLKNMADRVLAKLSSFGMDKMNVRI"
     gene            complement(8878..12588)
                     /gene="metH"
                     /locus_tag="PKZ49_04420"
     CDS             complement(8878..12588)
                     /gene="metH"
                     /locus_tag="PKZ49_04420"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HND35840.1"
                     /translation="MTHLRSTLLENLLTQRILLLDGAMGTMIQSYKLTEADYRGTRFA
                     DYPHDLKGNNDLLTLTRPDIIRSIHSAYLEAGADIIETNTFNSNAPSMADYHMEQLVY
                     ELNVAGARLAREEASAFERRNPEHPRFVAGVIGPTTKTASISPDVNDPGFRSITFDQL
                     VKDYSESIRGLVDGGVDILLVETIFDTLNAKAALFAIDQFFEQHHIRLPIMISVTITD
                     ASGRNLSGQTPEAFWNAVQHAQPLSVGINCALGAELMRPYVEELSNVANVYTSTHPNA
                     GLPNPLSETGYDESPEYTASLIKEFAQSGFVNIVGGCCGTTPAHIQAIAKAVEHIPPR
                     KIPDLPKKLRLSGLEPLNIGDDSLFVNVGERTNVTGSRAFARLILNDNYNEALNIARN
                     QVENGAQVIDINMDEAMLDSQKAMVTFLNLIAAEPDISRVPIMIDSSKWSVIEAGLKC
                     IQGKAIINSISLKEGEAEFIQHARLAKRYGAAVIVMAFDEKGQADTKARKIEICTRCY
                     RILVDQVGFSPEDIIFDPNIFAVATGIDEHNNYALDFIEATQAIKQSLPYVKVSGGVS
                     NVSFSFRGNEPIREAIHTAFLYHAIQAGMTMGIVNAGQLGVYSDIPKELLEEVEDVLL
                     NRRPDATERLVSFAENFKGQKKEQIEDLSWRNEPVRQRLAHALVKGISTYIEEDTEIV
                     RQEIADKGGRPIEVIEGPLMEGMNVVGDLFGAGKMFLPQVVKSARVMKQAVAYLLPYI
                     EAEKKSSGDNRPKGKIVIATVKGDVHDIGKNIVTVVLQCNNYEVINMGVMVPSAQILE
                     TARRENADIIGLSGLITPSLEEMAHVAKEMEREGFKIPLLIGGATTSRVHTAVKIAPH
                     YSGVTVWVPDASRAVGVCSQLMSQDQQVAYKAEVKAEQEKIRAQQKNKKGPAQLLTLE
                     EARNNAFKTDWRSYTPPSPSFIGIRTLNHYPLESLVPYIDWTPFFQAWELSGRYPAIL
                     DDEVVGEAAKNLYRDAQAMLKKITDQKWLTANAVIGLFPANSVNFDDIEIYADRSRNK
                     VLMTYHTLRQQTIKPAGRPNLALADFIAPKETGIIDTIGLFAVSAGFGIDERVKAFEA
                     AHDDYSAIMLKALADRLAEAFAEHMHARVRREFWGYIKDESLNNEQLIDEAYQGIRPA
                     PGYPACPDHTEKGPLFNLLEAEKRTGIIVTESFAMVPTAAVSGFYFAHPESDYFAVGK
                     IGKDQVEDYARRKGWSLSEAERWLAPILGYER"
     gene            12813..13400
                     /locus_tag="PKZ49_04425"
     CDS             12813..13400
                     /locus_tag="PKZ49_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HND35841.1"
                     /translation="MSEYHVLPTQRLVGAIRVSHPASPKSVTLESPALDIMTDLRHSH
                     VAVIEPYTTIEFANLYMKQRGVRSLFVLGTDQNLQGVITATDILGEKPLRFIRERNVK
                     HQDIVVSDIMTLLNELEAITIEDVQHAKVGHVIASLCEAGRQHTLVLERDPDDNLLIC
                     GIFSLTQIEKQLGSPIAVKEIAKTFAEIEATLIAK"
     gene            complement(13491..13895)
                     /locus_tag="PKZ49_04430"
     CDS             complement(13491..13895)
                     /locus_tag="PKZ49_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35842.1"
                     /translation="MKKIMLVILLLISHSVSAKWIFVGGSDGQNSFNLYTNPTMLRRT
                     SHIAQMWYLKDNLSTQYGYMGAQYLSSLSLGEYDCINLKFRVIAISYHSKNMADGETI
                     HLDTNPTDWSVISPGSIGKALFDIACNFKTTT"
     gene            complement(14230..15009)
                     /gene="gloB"
                     /locus_tag="PKZ49_04435"
     CDS             complement(14230..15009)
                     /gene="gloB"
                     /locus_tag="PKZ49_04435"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HND35843.1"
                     /translation="MIKINPIHAFKDNYIWIIHNEKYAVIIDPGIATPVIEYIRQYHL
                     KLIAILITHHHHDHTGGNLELSQLFGVQIYGPLKETIPGMTDGLEEGNIISLPELSLN
                     LTVLDIPGHTAGHIAYYDSNHLFCGDTLFSCGCGRIFEGTAQQMYHSLKKITALPDQT
                     QVYCTHEYTLSNIHFATEIDPTNSLLTEYANKVRMLRANNKPTIPTNLAIEKSINPFL
                     RCEHPAIINAANQHAETQLSDAISVFSEIRKWKDHYQPHVH"
     gene            15050..15799
                     /locus_tag="PKZ49_04440"
     CDS             15050..15799
                     /locus_tag="PKZ49_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HND35844.1"
                     /translation="MKTFNSITTFSGHAERMQQWFDTCLGQYLLEQEQQYFDHAVANI
                     FGYNALQIGCPQFDFLRLNRIPFKFNVGMSSGVSLQALPTFLPIESGSMDLTVLPHVL
                     EFSPQPHQILREAYRVLIPEGHIVISGFNPISLWGTPRYFKSLKNEFPWCGDFVGLAR
                     LKDWLKLLGFEIVGGRLGCYVPPFKKEKWLKRFCFMEAAGDRWWPISGGVYFLQAVKQ
                     VHGMRIIQPRWKKKFSSKKRIIPATQKLEDK"
BASE COUNT         4709 a         3038 c         3753 g         4448 t
ORIGIN      
        1 aaagtagaaa atggaaccca gttggttgac caagcaggaa gaaccatgga tgaagttgta
       61 atgtcggtaa aacgggtgac ggatatcatg gctgaaatat cggccgcttc ccaagaacaa
      121 agtgcgggta ttgaacaggt taatttagcc attagccaaa tggatgaaat cacgcaacag
      181 aatgcagctt tggttgagca ggcggctgcg gcatcggaat cgatgcacga acaggccgca
      241 caactggcac aagctgtcgc agtctttaaa ttacaacacg cagcacaagc tgcaaaacat
      301 cagggtgaga aaaaaattga ccgccgaggc cctgatcgtc ctgggaatgt tcagcgatta
      361 cctaaaaccg ctaatcaggc atcgaagaaa gaaacgatgc cagttgctaa gacgggtaca
      421 gatggggact gggaagagtt ttgatgcaga agatcgaaaa catgatagct acgattaact
      481 gagaacagac tatgagattg aatcttccgg tcaatgatgt tgaaagagaa atgagagatg
      541 gagagtttct tgtctctaag accaatttaa aaggtgtgat tacttatgtt aatcagcctt
      601 ttatagaaat gagtggttat acgaaagaag aactcattgg gcaagcacat aacttgattc
      661 ggcacccgga tatgccgccc gctgctttcg aggatttttg gaaaacactt aaacaaggca
      721 ggccttggag tggttttgta aagaatcgcg caaaagacgg atctttttac tgggtttttg
      781 ctaatgctac gccaattaac gaagctgggc agattactgg ctatctatcg gtacgtagta
      841 agccgacgcg tgaacaaatt agagcggctg aaaatttata tcgtgaaatg agggaggggc
      901 gtgcgaaggt taaaattaat gcgggcgacg ttgttccact gacattaatc gggcgattaa
      961 aacagcggtt acgatttgta agtgttcgta ctcgtatcat catgatgctc agttttttta
     1021 gcgtcataat tatctgccta gggattactg gtttattggg ctttaatcaa agtaatgaag
     1081 cgcttcgttc catgcatatt gatcgtactg gcgcattaga agatttatcg atgattctgg
     1141 ataaaatcca gcaaatccaa attcatagcc atgttgtaag tagccaaaat aacctagaag
     1201 tcgtgaaaaa acgctatgct atggttactg agcgtgagaa agagcttgat gagcgatgga
     1261 cagagtatct gggcacttac ctcttgccat tagaaaagga gctggctgaa acttttcact
     1321 cgcaaatgaa agcgttccga gaagccagtg caataaccct taacgcagct ttaaatggag
     1381 attacgagcg agcgtatcgc aatcaaatta acaccgtaga gccgaaattt gaattggctt
     1441 atacaacctt agtcaagtta attgaatttc aagcagcagc ggcgaaaaat gactatgagt
     1501 tagctgtcga gaagtttact ttaggccgaa attatgggtt gtttgtgatc gccttgtcga
     1561 tattcgtatc gatattaggg gggtatgccc tgttactggc gattactcga cctttagcaa
     1621 gattaaagcg gattgctgag aaaatttctg ggggaaattt taccagccag ataaccattg
     1681 caaattatga tgaaatcgga agtcttactg agacaatcaa aacattacaa atttgttctg
     1741 gtagtagttt agatgaggcc caacgcacag cagccgaaaa tttccgaatt agaacagtgt
     1801 tagatcagtc tactgcggca gtcatgattg ctgacagcgc gaatacgatt atttatatga
     1861 atcaaacgat gagaaaggcg ctgattgcat accaagcaga attccagaaa gtaattcaaa
     1921 attttaagcc cgatgcaatt gtaggcaatg actttagcta ttttcagcaa tcaatcaatc
     1981 tgcttggcct tactaaaccc acaaaacaaa ccattaatat tggagaaaga atttatttac
     2041 taaccctagt accagtgcta gatggttctg ggaatcgaat gggaacgtca attgaatggt
     2101 tagaccgaac cattgaagtc aaagttgagc aagaaatagc cagtgtcgtt ggagctgcag
     2161 gggaaggtga ttttagtaag cgtttatcat tagaaggtaa agaaggattt tttgcgcaat
     2221 tagcaagaaa tataaacaat ctagttgaaa cgagtgcatc tggcttaagt gaagtggtga
     2281 gagtacttgg tgctttatca aaaggtgatc tgactgaaaa aattaccaat gactataaag
     2341 ggatgtttgc caagttaaag gcggattcaa acgcaacgat tgagcagtta aagcatatta
     2401 tccataaaat caaagaatcc gcagattcga tcagcacggc ttcccaagaa attgcgcaag
     2461 ggaatatgga tttatcgcaa cgtacggaag cacaagcttc taatctggaa gaaactgctt
     2521 cgagcatgga agaattgact tcgacggtta aacaaaatgc agagaatgcc aagcaagcga
     2581 atcaattagc cgcatcggca tcggatgtcg cagtcaaagg aggagaagtc gttggtcaag
     2641 tggtagaaac catgagctcg atcaacgaaa gctcgaagaa gatcgtcgac attatcagtg
     2701 tcatcgatgg cattgcgttc caaaccaata ttctggcatt gaatgccgcg gtggaagctg
     2761 ctagggcagg cgaacaaggg agagggtttg cggtggttgc aacagaagta cgtacattgg
     2821 ctcaacgatc cgcatcggca gcaaaggaaa tcaaggatct gatcggggat tctgtaaata
     2881 aagtagagaa tggaactcag ttggttgacc aagcaggaag aaccatggat gagatcgtaa
     2941 tgtcagtaaa acgggtgaca gacatcatgg ctgaaatatc ggccgcttcc caagaacaaa
     3001 gtgcgggtat tgagcaggtc aatttagcca ttagccaaat ggatgaaatc acgcagcaga
     3061 atgcagccct ggttgagcag gcggctgcgg catcagaatc gatgcacgaa caggcagcac
     3121 aactgacgca agctgtcgca atctttaaat tacatcatgc ggctgggaca gcagtgcatt
     3181 gtaacgaaga aaagatggag cgccgaggtt cggaccgtcc tacaaatgtt caacgattac
     3241 ctaaagctgc taatcaggta ccagaaaaag aaatgatacc ggttgctaaa accggtacag
     3301 atggggactg ggaagaattc tagcaatctt tttgaacgtt gtttcataaa aatttaaata
     3361 atagttgagt gacagtatgc gtatcaatat gccaatcaat aatgtagaac gacaagtcaa
     3421 tgaaaatgac tatttagttt ctaaaactaa tactaaaggt gtgattactt atgttaatca
     3481 accttttata gaaataagtg gttatacgaa agaagaactc attgggcaag cacataattt
     3541 gattcgacac ccggatatgc ctcccgcagc tttcgaagat ttttggaaaa ccatccagca
     3601 gggaagacct tggagtggcg ttgttaagaa ccgggctaag gatggctcat tttattgggt
     3661 atatgccaat gtggcgccga tttatgaggc agagcgaatt accggctata tgtctgtccg
     3721 tagtaaaccg gctcgagagc agatacagca ggcagaatta ctttatcgtg agatgcgtga
     3781 aggtagagcc aaatttaaga ttgaagctgg agaattgata ccagccaact ggttcggcca
     3841 tatcaagcaa aagatgcgtt catttaccat taaatcccga atcaacgtca tcagttgttt
     3901 ggctattgtt agtttgagtt tcatgggtgg atggagtctt ttaggggatt atcaaagtaa
     3961 agagcggcta cgcgatgttt atgaaaaccg tacattagta ctaatagatt tatcaacgat
     4021 tctagatgca atacagcgca tgcgcgtcaa tgctttgaca gccgcaaatt ctaataatcc
     4081 agtaattgca aaggaacgat ttgcagatac gaatcaattg gatcgcatct ttgatgaaaa
     4141 ttggtcacga tatagtgtta cttcggttac gacagaagaa aaagtgaaag ctgaagaatt
     4201 ttatcggaat gtagaagcct atcgaatttc acgtgacaag acgatgaatt tgtctattga
     4261 gggagacttt gaagctgcta gggaaaatgc ggttaaatat gctcggccaa aatatgactt
     4321 agtgcacgcg gcgcttgttg atttatttga aatacaggaa aaacaaacga aagtagtatt
     4381 tgatactgcg attgaacaat ttgagatact caaaatttca acgatcgcca tgattttatt
     4441 ttttacattc atttatttgt acctcaccag actgctgcat cgagccatta atctaccctt
     4501 agcaagcgta agtgccattt ttaggaagat tgctgcaggt gattatacca atcaagttgc
     4561 cattgttaat cacgatgaaa ttgggtgctt aaccgagtcg gttcagatga tgcaaacgcg
     4621 ggcgggtgct gagatcgatg aaatcaagcg agtggctgct gaaaatcgac gtatccgcaa
     4681 tgccctggat caatctaatg catcaattat gatcgtcgat cccaatagct gcatcatcta
     4741 tgtcaatcaa gcgatgcaga tattacttaa aaataatgaa aaggaatttg tggctgtatt
     4801 agctggagtg cagattaatg cgatattagg ccaatcattt accattttta atgaaaaact
     4861 tgatctttct gaaattaata aatcaaccaa gcaattattg aaaatcggta gcagcatttt
     4921 tgtattgacg cttactcccg tattcgatga caacgggtcc cggctaggaa catccatcga
     4981 gtggcttgat cggacagctg aggcggctgt ggaagatgaa gtagcccaga ttgtaaaatc
     5041 agcgagcgag ggtgatttta gcaagcgcct agaaacaaaa agtaaaaatg gtttctttga
     5101 acaattagcc aaaaatatta atcaattact cgaaaataat gagaaagggt taaccgatat
     5161 tgcacggatg ctaggtgcga tggctaaagg cgatttgact caaaccatta cccggcaata
     5221 taaagggatg tttgaaaaat tgaaaactga tgcaaatgcg acggttcaac aactgacgga
     5281 cattattcga caaatcaagg aatcggctga ttcgatcaat atcacctccc atgagattgc
     5341 acagggtaat actgacttat cgcaacggac agaacaacaa tccatcagtc tcgataatac
     5401 tgcttctagt atggaggagt tgaccgcaac cgtaaaacaa aatgcggaga atgcaaagca
     5461 agccaatcag ctcgcggtat cggcatctga tgtggcaatc agaggagggg aggtggtggg
     5521 gcaggtagta gaaaccatga gttcaatcaa tgaaagctcg aagaaaattg ttgacattat
     5581 tggtgtgatc gatggcattg cgttccaaac caatattttg gcattgaatg ctgccgtaga
     5641 agcggccaga gcgggtgaac aaggtcgggg attcgcagtc gttgcaacgg aagtgcgtac
     5701 tttagcccaa cgttcagccg ccgctgcgaa agagatcaaa tctttaattg gagactcggt
     5761 gaacaaagtg gaaaatggca cccagttggt tgatcaggcc gggagaacca tggatgagat
     5821 cgtaatgtca gtaaagcgtg tgactgacat catggctgag atatcagcag cttctcaaga
     5881 acaaagcgag ggtattgaaa aagtcaatgc aacgattact caaatggatg agattaccca
     5941 acagaacacc gctttagttg aagaaggcgc tgctgcatcg gaatcgatgt atgaattggc
     6001 aaatgaactc accaaactcg ttgccgtgtt cagaaccaat tcatcaattt cattgattgc
     6061 tgaggctgag actgagtctg ggcaaattga gcgcagaggc cctcatcgag cgacgaatgt
     6121 acagcggctt ccactatcag caaaattagt gactaactcg attgaagata cggtggcagt
     6181 aacttaaacg aaggtaattg ccatcaaaag gctaaagaca gtttagcgtc atatcattta
     6241 atttaactca gtaatgacta cttatgaatc tcgatccggt tatagatcaa ccaatgcgtg
     6301 agtatgattt tacaaatcag gattttgctt gtgttaagcg gctaatttat cagcgtgccg
     6361 gaatttctct ggcaccgagt cgcagtaata tggtttatac ccgcttggtt aagcgattga
     6421 gagaaaccgg ttatagccag tttaaagatt atttagcgat gttggaggaa aatagcgata
     6481 gcggtgaatg ggaaagtttt gtcaatgcat tgacgacaaa cttgacctca tttttccgag
     6541 aagcgcatca ctttgagatg ttgacggagc atcttaaacg aagaaaacca gggcaaacga
     6601 ttaaaatttg gtgcagtgct tcatcaacag gagaagagcc ttattcgctt gcaatgactg
     6661 ctgtggaaac gtttgatacg tacacgccac ccgtacgtat tctagcaact gatattgata
     6721 ccaatgtgtt agcaacagcc caagcaggaa tttattccat gaaccaactg gaaaattttt
     6781 ctgatgcccg attgaggcgt tttttcctta aaggaaaagg gtctaatgct gcttttgccc
     6841 gtatccgccc tgagctaaaa aatctagtag cgtttaaaca gcttaattta ttagaccgat
     6901 cttggccgct taaagggcct ttcgatgcga tattttgccg caatgtcatg atttattttg
     6961 ataaaccaac tcaacaccaa ttactggata agtttgcacc gttgctcgct acggatggat
     7021 tattgtttgt aggtcattca gagagttttc agtataacga gaatccattc aaactgctgg
     7081 gcaaaacggt ttatgagttg ataaataaac attcacgatg accactatcg atagcgatca
     7141 ccttgctaca actgcctatt atgatcccat ccatcagatt gatgcggtaa aactattacc
     7201 gggtgagtat tacgttacgc agaaggacat attattagta acggtgttag gttcctgtgt
     7261 ggcagcttgt atctgggata gcttattgag aattggcggg atgaatcatt tcatgttgcc
     7321 aggcgatgaa aatagtggat cttcagcgcg gtatggggat ttcgcaatgt cattgctcat
     7381 cgaacagtta ctgaagctcg gtgcgcgtaa agagaatttg aaagctaaag tatttggggg
     7441 cggtaacgta ttgcctggtt ttgcagtaac cagtattggt gagcgtaatg cggaatttac
     7501 ccttgattat cttcagcatc aaggaatacc cattattgca cacgatttaa ccgatgtttt
     7561 tccacgaaag atttattttt ttcccagaac aggtcgtgca ttggtcaaaa agttacatgt
     7621 caaaaataat acgatttttg agcgagaaag gagtcattta gcagaaatta agcaaggatt
     7681 tgcattgact cgttcagtga tgagttaaaa aaagaaaaag atgaataagc ttcattttgt
     7741 aatgaagtaa tttttccagg aagggcatca tgaaaaaaaa gattagtgtg ttgataattg
     7801 atgattctgc tttagttcgt aacctacttt ctagcatcat taatagccag gaagatatgg
     7861 aagtgatagg cgccgcgccc gatccactga ttgctcgtga gatgattcgt cgattaaatc
     7921 ccgatgtgct aacgctggat atcgaaatgc ctaagatgga tggtctcgag tttctggaaa
     7981 aattgatgcg cttacggcca atgccggtta ttatgatctc tacactgacc gagaagagtg
     8041 ctgaagcaac catggaagca ttagcactgg gagctgtcga ttttgtttcc aagccgaaag
     8101 ttgatgtcgc gagtgagcta cagctatatc gtgaagaaat cgcacgtaaa attcgcatgg
     8161 cggtttcagt acaaataaaa aaaccaacgc ctgtatcgac tgacacaaat caaacagctt
     8221 cttctgcctt tggagctaat cgtatctatt caacggaaaa gttaattatc ataggttcct
     8281 ctaccggggg aactgaagca ttaaaagatt tcttggtgaa cttaccaccg catacacccg
     8341 gtatattgat tgctcaacat atgcccgaag cgtttactaa atcatttgca actcgtttgg
     8401 atagtctttg caagatttca gttaaagaag ctcagggggg agagagagtt ctaccaggac
     8461 atgcttttat tgctcctggc cattctcatc tgctattgaa gcgcagtggc gcaaattatg
     8521 tgacggagct tagccaatcg gagcctgtga atcgtcatcg accttcggtg gatgttttgt
     8581 ttcgctcagc agctacgtat gccggcaaaa atgcgattgg agttattctg acaggcatgg
     8641 gtagagatgg ggctgccggg atgctggaaa tgcgtaaagc aggtgcttat aactttgccc
     8701 aggatgaagc gagttgtgtg gtattcggta tgcccaaagc cgcaattgag gtgggtgcaa
     8761 ccgatgaagt tgtgcccttg aaaaatatgg ccgatcgtgt actcgcaaaa ttaagcagct
     8821 ttggtatgga taaaatgaat gtacggattt gaaccttgag tatatccatg ctcggtttta
     8881 tcgctcataa cctaagattg gtgcgagcca tctctcagct tcactaagcg accagccttt
     8941 tcgtcttgca tagtcctcta cttggtcttt tcctattttt ccaacggcga aatagtctga
     9001 ttcagggtgg gcgaaataaa aaccagaaac tgctgcagtt ggaaccatcg cgaaagactc
     9061 cgtaacgata ataccggttc gcttttctgc ttcaagtaag ttaaataatg gtcctttttc
     9121 cgtatgatca gggcaagcag gataacccgg tgcgggccga atgccttgat aggcttcatc
     9181 aatgagttgt tcgttgttga gtgattcgtc ctttatatat ccccagaatt ctcgtcggac
     9241 tcgagcgtgc atatgttcag caaatgcttc tgctaggcgg tctgccagcg ctttgagcat
     9301 aatggcacta taatcatcat gggctgcttc aaaggcttta acccgttcat cgatgccgaa
     9361 accagcactg acggcaaata agccgattgt gtcaataata ccagtttctt tgggagcgat
     9421 aaaatcggct agtgccaaat taggacgacc tgccggtttg atcgtttgct ggcgtagcgt
     9481 atggtaagtc atgagcactt tattacgcga gcgatcggca taaatttcga tatcatcgaa
     9541 attgacagaa ttagcaggaa acaatcctat aactgcgtta gccgtcaacc atttttgatc
     9601 cgtaattttt ttcagcatgg cttgtgcatc gcggtataaa tttttagcgg cttcaccgac
     9661 gacttcgtca tctaagatgg caggatagcg acctgataat tcccaggctt ggaaaaaagg
     9721 tgtccaatcg atatagggaa ccaatgattc gagggggtag tgatttaagg ttctaatccc
     9781 gataaaactt gggctgggcg gagtataact acgccagtct gttttgaaag cattgttgcg
     9841 cgcttcttcg agtgtgagca gttgagctgg gccttttttg tttttctgtt gtgctcggat
     9901 tttttcctgt tccgccttaa cttcagcttt ataggcaact tgctgatctt gggacatcaa
     9961 ttgactacat acgcccacgg ctcgactggc atcgggtacc cagacagtta ccccgctgta
    10021 atggggagcg attttaacgg cagtatgaac ccgagaagtt gttgctcctc caatcaagag
    10081 tggaatttta aagccttcgc gctccatctc tttggcaaca tgcgccattt cttcgagcga
    10141 aggggtaatg agtccagata agccaatgat atcagcgttt tcccgacgcg cggtttccaa
    10201 gatctgggca ctgggaacca taacgcccat attgattact tcgtagttat tgcactgcag
    10261 tacgacggtt acgatatttt tcccgatatc gtggacatcg cctttgaccg ttgcaatgac
    10321 aatctttcct ttagggcgat tatcacccga actttttttc tcagcttcaa tataggggag
    10381 taaatacgca accgcttgtt tcatgactcg cgctgattta acaacttgcg gcaagaacat
    10441 ttttccagcg ccaaataagt cacccacgac attcatacct tccatgaggg ggccttcgat
    10501 gacttcgatg gggcgtcctc ctttatcagc aatttcttgt ctaacgattt cagtatcttc
    10561 ttcaatgtaa gtactgatac ccttaaccag ggcatgtgct aagcgttgac gaacgggctc
    10621 attgcgccaa gaaagatctt cgatttgctc ttttttctgc cccttgaagt tttctgcaaa
    10681 actaacgagt cgttcggttg catcaggacg tcggttgagt agtacgtctt ctacttcttc
    10741 taagagttct ttggggatat ccgagtaaac gccaagttga cctgcattga caatacccat
    10801 ggtcattccc gcttgaattg cgtgatatag aaatgctgta tggattgcct ctcgaatcgg
    10861 ttcatttcct cgaaatgaga acgatacatt ggaaacccca ccacttacct tgacgtaggg
    10921 cagagactgt tttattgctt gggttgcttc aatgaaatca agcgcatagt tgttgtgttc
    10981 atcgattccg gtagcaactg cgaaaatatt gggatcaaag ataatgtctt caggagagaa
    11041 tccaacttga tcaactaaaa ttcgatagca tcgtgtacag atttcaattt tgcgtgcttt
    11101 cgtatcggct tgtcctttct catcaaaggc catgacaatc acggcagcgc cataacgttt
    11161 tgctaatcgg gcatgttgga taaattctgc ttcaccctct tttaaactaa ttgaattgat
    11221 gatcgccttg ccttgaatac atttcaagcc agcttcgatg actgaccatt tgctggagtc
    11281 aatcatgatc ggtacccggc tgatatcagg ttcggctgct atcagattga ggaatgtcac
    11341 cattgctttt tgggagtcca gcatagcctc atccatgttg atatcaatca cttgagcgcc
    11401 attctcgacc tgatttcggg cgatattgag tgcctcattg tagttatcgt ttaagatgag
    11461 tcgcgcaaat gcccttgatc ctgttacgtt cgtacgctca ccgacattga cgaaaagtga
    11521 gtcatcgccg atattgagcg gttccaagcc ggataagcgt aactttttag ggagatcagg
    11581 tatttttctg ggtggaatat gttctaccgc tttagcaatc gcttgaatat gggcaggcgt
    11641 ggtgccgcag catcccccca cgatattaac aaacccagat tgggcgaatt ctttgatcaa
    11701 gctagccgta tattcgggcg attcgtcata gcctgtttca gataatggat taggaagtcc
    11761 tgcattggga tgggtactcg tataaacatt ggcaacattg gaaagttctt ccacataagg
    11821 acgcattagc tcagcgccca atgcgcagtt gatacccacc gataatggtt gggcatgctg
    11881 taccgcattc cagaacgcct caggtgtttg tccagagaga ttgcggcccg atgcatcggt
    11941 aatggttacg gagatcatga tcgggagtcg aatgtggtgt tgctcaaaga attgatcgat
    12001 cgcaaataac gctgctttgg cattcagcgt gtcgaaaatt gtttcaacca gaagaatatc
    12061 gacgccacca tctaccaacc cccgaataga ttccgaatag tcttttacta attgatcgaa
    12121 agtaattgaa cgaaaacccg ggtcattgac atcgggtgaa atcgatgccg ttttggtcgt
    12181 cggcccaatg acacctgcga caaaacgagg gtgctcggga tttctacgct caaacgctga
    12241 agcttcttca cgggctaatc ttgcaccagc gacattaagt tcataaacca attgctccat
    12301 atgataatca gccatggagg gtgcgtttga attgaaggta ttggtttcga tgatatcggc
    12361 gccagcctca aggtaagcgc tgtggattga gcgaataatg tcaggtcgcg tcagcgtcag
    12421 taaatcatta ttccctttga gatcatgggg gtaatctgcg aaacgtgttc cacgataatc
    12481 tgcttcagtt aatttgtagc tctgaatcat ggtacccatt gcgccatcaa gcaataagat
    12541 acgttgggtt agtaagtttt cgagtaatgt agaacgaaga tgggtcatag cgtaaaaaat
    12601 tatctggcgt gaggaagctt attatacctg atgacttagc gactcattgc gattcgagat
    12661 aagctaaaaa gtcagacgtt tgatttgttg agtcaatttg aaaatggaat attgacctgc
    12721 taatttgttc ggcgtagagt aaccgagcaa ttgattgagt gtttggtttt tcccttaaat
    12781 attaaacctt taaattcaac aggaggattg ttatgagtga atatcatgta cttccaactc
    12841 agcgtttagt aggtgcaatt cgagtatcac atcccgcctc acccaaatct gtaacacttg
    12901 aatcacctgc gctcgatatc atgactgatt taagacacag ccatgttgca gtgatagagc
    12961 cgtatactac cattgagttt gctaatttat atatgaaaca aaggggagtg cggtcattat
    13021 ttgttttagg aacagatcag aatctccaag gcgtcattac tgctacagat attctaggag
    13081 aaaagccatt acgttttata cgggaaagaa atgttaagca tcaagatatc gtcgtatccg
    13141 atatcatgac gctcctgaat gaattggaag caattacgat tgaggacgtc caacatgcca
    13201 aagtagggca tgtgattgca agtttgtgtg aagcggggcg gcaacatact ttggtgctgg
    13261 aacgagatcc agacgataac ttattgatct gtgggatatt ttctctgaca caaattgaaa
    13321 aacaactagg ttcaccgatt gcagtgaaag aaatcgcaaa aacctttgca gaaattgaag
    13381 caaccttgat tgccaaatag cttacggatt acattctttt taagacaggg tactggttgc
    13441 gcgcaaatca gtaccttttt tctgagactt aataaatacg atcagtggac ttaggtcgtt
    13501 gttttaaagt tacaagcaat atcaaataac gctttaccaa tactaccggg tgaaatgact
    13561 gaccaatcag tagggttggt gtcgagatga atcgtttctc catctgccat attcttactg
    13621 tgatatgaaa tagcgatgac tcgaaacttg agatttatgc aatcatattc tcccagcgac
    13681 aagctcgata aatattgagc ccccatatac ccatactgag tggaaagatt atcttttagg
    13741 taccacatct gagcgatatg gctagtacgg cgtagcatgg tcgggttggt gtaaagattg
    13801 aaactattct gaccgtcact tccacccaca aaaatccatt tggctgatac cgagtggctg
    13861 attaatagca agatgacaag cataattttt ttcattacct ttcctatggt tgatgtcagt
    13921 gaatcaagtt acccaattcg atgttcagtt ttaataagcc aatagtcgat ttcagtaata
    13981 tggcttgaga taaggcattt ccattgtttt agcggggtat cattgcggaa atgtcacggt
    14041 ttctcctgca atgcaactca agttagcttg agtatttcaa gcattaatta aatcaaagcg
    14101 gaaaattcgg gcaggttttg attttgaatt aggcctgtga aagcctgctt tttgagtaat
    14161 tgattggagg tggattaact agaagtgttg attgctttga atctgagtcg aatgattgca
    14221 aattaattat caatgaacat ggggttgata atgatctttc catttacgga tttcagaaaa
    14281 cacgctgatt gcatcggata attgtgtttc tgcatgctgg tttgcagcgt taataatcgc
    14341 tgggtgttcg catcttaaga agggattgat cgatttttcg attgccaggt tcgttgggat
    14401 ggtaggcttg ttgttagcgc ggagcattct aactttgttt gcgtattcag taagtaatga
    14461 attggtagga tcgatttcag ttgcgaaatg aatatttgaa agcgtatatt cgtgtgtgca
    14521 ataaacctga gtctggtcag gtaaagcggt gatttttttt aatgaatgat acatttgttg
    14581 ggccgttcct tcgaatattc gcccacaacc acaagaaaac agcgtatcgc cacaaaacaa
    14641 gtgatttgag tcgtaatagg caatatgacc agccgtatgg cccggtatgt ctaatacggt
    14701 caaattgagt gatagctcgg gaagactaat aatattacct tcttcaagac catcggtcat
    14761 tccgggtatc gtttctttta ggggaccata aatttgaacc ccaaataatt gagacaactc
    14821 taagttacca ccagtatggt catgatggtg gtgtgtaatt aagatggcaa tcaatttaag
    14881 atggtattgt ctaatatact cgataacagg agtagctatt cctggatcga taatcacagc
    14941 atacttctcg ttatgaatta tccagatata gttatcttta aacgcatgga taggattgat
    15001 ttttatcatg attttcctaa tgaacggtaa gtggtatcaa aggtaaagaa tgaagacatt
    15061 taacagtatc acaacttttt cgggacatgc ggagagaatg cagcagtggt ttgatacttg
    15121 tctaggtcaa tatttattag aacaagaaca acagtatttt gatcatgccg ttgcaaatat
    15181 ttttggctat aacgcattac aaattggatg tccgcagttt gattttctgc gcctaaatcg
    15241 tattcctttc aaatttaatg tgggaatgag ctcgggtgtt tcgttacagg cgttaccgac
    15301 attcttaccg atcgagagtg gcagtatgga tttgacggtg ttaccccatg tgctggaatt
    15361 tagtccccaa ccccatcaaa tattacgtga agcttaccgc gttttgatcc cagaaggtca
    15421 tatcgtcatt agtggtttta atccgatcag tttatgggga acgccacgtt atttcaaatc
    15481 attaaaaaat gaatttcctt ggtgtggtga ttttgtaggt ttggctcgac tcaaagattg
    15541 gttaaagtta ctcggattcg agattgtggg cggacggctt ggctgttatg tgcctccttt
    15601 taagaaggaa aagtggctta agcgattctg ttttatggaa gctgctggcg atagatggtg
    15661 gcccatttca ggaggggttt attttttgca agcggttaag caagtgcatg gaatgcgaat
    15721 tatccaacct cggtggaaaa agaagttttc aagtaaaaag agaatcattc cagcaacaca
    15781 gaaactcgag gataaataaa tagttatgga taatcttaaa aattactggg gtgattacgc
    15841 gccttcatct tgacagaaga gcgtataagc aagtaggctt tggattgaaa taaaatcgaa
    15901 caggaagtaa cgggaagagt taagaaaagg aggagagaat agatgaag
//
LOCUS       DAOQKQ010000045        15784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_1714_length_15784_cov_6.202365, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000045 DAOQKQ010000000
VERSION     DAOQKQ010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 15784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15784
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1714_length_15784_cov_6.202365"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..532)
                     /gene="gspG"
                     /locus_tag="PKZ49_04445"
     CDS             complement(98..532)
                     /gene="gspG"
                     /locus_tag="PKZ49_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HND35845.1"
                     /translation="MKGCYTNLGKRTIQGFTLLELLVVMVIIGLLAAYVGPKYFSQVG
                     KSEVKMAQAQIDSLEKALHQYRLDVGQYPTSEQGLNALVTRPGNELKWQGPYLSKMPP
                     PDPWGRPYQYKYPGDRAEFDLFSLGKDGQPGGTGDAADIANW"
     gene            1011..3155
                     /locus_tag="PKZ49_04450"
     CDS             1011..3155
                     /locus_tag="PKZ49_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35846.1"
                     /translation="MVDLSDIDLTYKLVIGLFNAAPGKANLASLLSAIDDGLSLPQIG
                     DRLDSTLLFNQRIIGDLSEADQVSLIMSHFGFVHGQSTGNERKQVRDYLTGRLKSGDS
                     WGQIVYDAVVYLSGNPDPMFAKAALLLSNKVLVSSLFSQSYSEDSLEVLQSVLSGVSA
                     DSLLDEVSAEAYLAEIGKPVGAVNLTVAKDVLSSHVILAPRGYTPAGTDQINTLNDDD
                     VLSGTASEIDKLVFDFVNDADTGDHNIVPQLSGIEILDIKFSLDGAGTLDLQDTVGVT
                     HIDLHRIDDGATAIIDNITEATTNHLSINRSNVTDSNIAFTYLDSALIGTDDVVTLTL
                     NNAQAGVVSIQESTDDGSLDQGFETINVISSGDQANTLISLTTEDAQLLNITGNQDLT
                     IQALSGTNQSSLVGLQNMGGSLTKIDASTFAGNLRININGLLDAVLDDSSGKDVPVTV
                     LGGIGNDTIWLSSKNNVNDELNGGTGVNTLILGGGFDSATVTELNPDKLKNIVNFSTL
                     ASTNFSLTPELLKLQLDDFVLDSLVDSEHISSIEEPETLTLRINDFQDIETPQAAVAL
                     QLDISDLTQQSAVNVTLDTGSPHYQLEHDTIQLSPHGSSITINNFQTLSDGAVVDVFG
                     GSAADQIILSLADFKLVDSSAGNNIAITDFLFAFDTIDPASDPSVEAIIFDSTTGNLF
                     YNPDYATEGGLVLIASLMNISDLTDLDIFILS"
     gene            3340..4089
                     /locus_tag="PKZ49_04455"
     CDS             3340..4089
                     /locus_tag="PKZ49_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35847.1"
                     /translation="MQKNSSSKNMSRALIAMGLIIAASQVSAHTRLQVPQIPEGTRVY
                     NNVVVGHGCEGKDVIATTAVFPDGVDSTVLVDGNPYEGSISEFINWGNLIQKVMSKDV
                     FAKEAEKYDQSGSNVVGFWVRDGALPATYTGLFPFRSDAAVIEPSSCAKSVKVAVAIA
                     DICKNTNIGGFTDLTVNLWTPAVGSNFDGPGLHGYNSPATLTINRDLAANPLDASCGE
                     GSAVELLPSAAQLDRDMPIKNKDGGVWWPKN"
     gene            4223..4783
                     /locus_tag="PKZ49_04460"
     CDS             4223..4783
                     /locus_tag="PKZ49_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HND35848.1"
                     /translation="MLFSKRNKAQWIVLIPWFIAAQMSAASASEQDLSRETKAHAASA
                     ARIHCSDNSAYQREPDPELTASIKSMAESTDSSERLVALAQLSEQRAIDPEVADQILY
                     IALLDDDPIIRGQAVYAIGRRGGMDASIVIDQALADEVPSVRLMAVDALAASDEESNA
                     LLRKALEDESAAVRELASTKLGLVAK"
     gene            4865..5329
                     /locus_tag="PKZ49_04465"
     CDS             4865..5329
                     /locus_tag="PKZ49_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35849.1"
                     /translation="MKIFKNKFVLISSLLGAAFIFSGNVAAHNQAGSFTTGLANAVDF
                     YQVTCSDDGSGAPSYLEFQVKDTTANTAKVQALVQKGTSCGVNACAVTTLDTIDTDAL
                     YSPLAQVKQGAGVYNLFVSHTSTGADAYDVLLHCKTAAGVHTGTSVVSRQQK"
     gene            5552..6310
                     /locus_tag="PKZ49_04470"
     CDS             5552..6310
                     /locus_tag="PKZ49_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35850.1"
                     /translation="MNKHFRINQLFQTFLMIGILIHFSQSVLAHTLFDIPRLPELKKT
                     TNHITIGHGCQGNTVIGTSVVFPDGQDSTITIDGNAHSGTLDEFVSNWGGIFQLLQDR
                     SVFAEQDVKQDGNGNTTGFWSGGGRGMAAHLNAQVPFTMSAVLLNSESCAKSVRFAVA
                     VVDICTITTFADFADDTVGLWVPAVGSQFDGNPGGHAYDSPAYLTIVRDLEGNPLPES
                     CGEGIEEVLVKPSAAQINRDMPIRFNGQQVWPQN"
     gene            6486..6995
                     /locus_tag="PKZ49_04475"
     CDS             6486..6995
                     /locus_tag="PKZ49_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 domain-containing protein"
                     /protein_id="HND35851.1"
                     /translation="MVRRTLTALAASLLLLPVSVLAAGYGPAGCGLGSMVIGSKPGME
                     QVFAATTNGTSGSQTFGITSGTSNCGDHGLVNLSKEREVFAQENYTSLVKEMAQGKGE
                     SLSTLASLYQCPATTHGEFGTLVQEKFDYLVANDQTTSAELLSKLDGQLMQHPNLANS
                     CNIKLSALN"
     gene            7061..8974
                     /locus_tag="PKZ49_04480"
     CDS             7061..8974
                     /locus_tag="PKZ49_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HND35852.1"
                     /translation="MSYRILSFGLVFCFFLFSHLALANNEEALRYLIELQQQAQQKKL
                     AQARVWHRLLKYNPQLLRGVMSEADGLDFFNAPQGKIDSDAELAATLASFFLPLSDVP
                     EGKEHPQCNFPARFKWLNQQLSFDPQRLDIQSCDRLDRWLGVLDPVGVTLVFASYFMN
                     NPASMFGHTLIRIDSRRTGPDNHLINYGANYAAIPDTDNAFLYALKGLIGSFQGRFAI
                     FPYYTKVQEYNNWESRDLWEYELQFNEDQLHMLLLHLWELGGTYFDYYYFQENCSYHV
                     LSLLEIANPDLFLKDSFFFSVIPTDTVKVVLAQEGLVKQVVYRPAVLNLMNDKRLRMT
                     SDERKLFEALIKGNITIENPSFTELPVRSQANLLNAYMDYRQYYSMQEDSNHEIKIPR
                     AVLLARSRLPPTEDNDQTIRFSSPPHQGHGTDRFRLGWGHNDREPFVEFAYRPAYHDF
                     MAKNVGYDKNSEIIFMDFKLRYYLESERVRLDQAKLLSITSFNPYDPLFARTSWRGEI
                     EIDTLRDRDCHYCNSFKGSYGRGMAYRPQFFSPLLLFSFIDVKTEFSPHLNQNYRLGG
                     DVEVGAYYDITDNLRVKLAGSYQVFFLGESKRFFTAQFLTRYAIAQDLDIRMELNRYD
                     HNHEGIFAINYFF"
     gene            complement(8958..9818)
                     /locus_tag="PKZ49_04485"
     CDS             complement(8958..9818)
                     /locus_tag="PKZ49_04485"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HND35853.1"
                     /translation="MKAFRLLMLLSTWLLTSCSSVLYYPSQNRLYYDPKSVGFSPTDI
                     YFTDAAQRKLHGWWFPSTITPAKATVIFFHGNAENLTSHFMHLAWITAEGYNLFIFDY
                     PGYGLSEGKPTPQSCVEAGHAALDWVLSHDPENLPMIVYGQSMGGIIALRTVIDKRED
                     INLKLVVAESTFDSFQQIARHKLSQHWFTWPLQPLSYLLLSDRWAPTHLETIAPVPVL
                     VIHGQQDNVVEPQFGEAIYEKLSEPKSIWRIPNGNHTDIFWQHEKKYRTVFLNFLTQL
                     DLNQSVRKNN"
     gene            complement(10174..10764)
                     /locus_tag="PKZ49_04490"
     CDS             complement(10174..10764)
                     /locus_tag="PKZ49_04490"
                     /inference="COORDINATES: protein motif:HMM:NF038127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DVUA0089 family protein"
                     /protein_id="HND35854.1"
                     /translation="MKKMFLAVCIVFALSVAQVGAAPISSSFTGSFNTDDDVEFFSFA
                     VTEPSLVTLRTWSYAGGTNAEGNTISAGGFDPIITLFNGTGNLIDDNDDGAGVAADPV
                     TGAEFDAVLSIFLDIGNYTASLTQYANFAIGPNLADGFTGSGVTGFIDADGNTRTSAW
                     AFDIVISELPEPTSVALLGLGLVSLAFTRRSIKKIS"
     assembly_gap    11098..11197
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11281..11721
                     /gene="msrB"
                     /locus_tag="PKZ49_04495"
     CDS             11281..11721
                     /gene="msrB"
                     /locus_tag="PKZ49_04495"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HND35855.1"
                     /translation="MINWSQILMLAKLGNPPPSQRVEKTEAEWKSLLTDEQYFVLRQK
                     GTEPPHSSKLCTQFEPGLYACAGCGTELFDASSKFNSGTGWPSFTQPVAENVVAYYLD
                     TSYGMQRIEVNCNICGGHLGHAFPDGPEPSGLRFCINALSLKKV"
     gene            11748..12758
                     /locus_tag="PKZ49_04500"
     CDS             11748..12758
                     /locus_tag="PKZ49_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HND35856.1"
                     /translation="MTLQHNPLIASQLPIRLVIHGGAGAISRARFTKEREQTYQQTLR
                     EALLAGYAVLNAGGSSIEAVTEAIVVMEDSPLFNAGKGSVFTHEGHNELDAAIMDGAN
                     RMAGAVAGVKTIRNPIRAARAVMTKSNHVMLIAEGAEAFARDQRLEIVDATYFYTQQR
                     WEQLQRTIERERVQHQQAIEADSLLVEKDDKYGTVGAVALDRSGNLAAGTSTGGLVNK
                     RYGRVGDSPIIGAGTYADNRSVAVSGTGTGEMFIRTAAAHSIATRVQLKQLSITEAAD
                     QILAEIAAIGGDGGVIVLDKSGHYTMRFITSGMYRGTIGGDGVPWVGIFPEAEIALNA
                     EK"
     gene            complement(12804..14114)
                     /locus_tag="PKZ49_04505"
     CDS             complement(12804..14114)
                     /locus_tag="PKZ49_04505"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HND35857.1"
                     /translation="MIKKPRVGQNYQFMLAGLVLLCLSVLTFTVHANSSAQVTSTRVW
                     AGPEYTRVTLESNLPIKYTLSLQSSPDQIVLDLENTGLNPPLGSLPTKIDAKDPLIKK
                     AHIEYLSSNGVRLTLALKTKAVPRTFTLDPMDKFGYRLVLDIFPPDRAATKPNGHHDD
                     PLMALVKASEEKIATKAKNANKPEITLASFNPRPEKQKTIIVAIDAGHGGKDPGAIGP
                     RGSQEKDITLSIAKKLKAKIDKEPGMHAVLTRTGDYFLPLAVRRTKARSANADLFISV
                     HADAVPRNDARGSSVYTLSENGATSTTASWLAQKENESDRIGGIKLNTKDHYLRKTLI
                     DLSINATINDSIKLAGTVLSAIGNVNHLHKRQVEQAGFAVLKSPDIPSILVETAFISN
                     PTEEEKLKSENYQDRMASAIVSGIKRYFSNSRAIPARTELAKVN"
     gene            complement(14111..14608)
                     /gene="tsaE"
                     /locus_tag="PKZ49_04510"
     CDS             complement(14111..14608)
                     /gene="tsaE"
                     /locus_tag="PKZ49_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HND35858.1"
                     /translation="MHNRDCSEAVPNEEQQYCLHLIDEAATLAFGAKIATTLHAGLII
                     FLKGDLGAGKTALTRGILRGMGYQGKVKSPTYNLVEIYNFSRLYLYHFDFYRFNDYSE
                     WEAAGFRDYFNSETICLVEWPEKGGDLLPTADLTIKLQFANRGRFAGICANTTQGKKC
                     LALLA"
     gene            14603..15688
                     /gene="queG"
                     /locus_tag="PKZ49_04515"
     CDS             14603..15688
                     /gene="queG"
                     /locus_tag="PKZ49_04515"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HND35859.1"
                     /translation="MQEEISQNGTVDPVSLKQSITNWAHQLGFQDIRIANAEADMSSV
                     ETGLSTWLAMGFHGDMDYMAKHGIKRTRPNELVPGTSRVISVRMNYLPSDVKESWSVI
                     EDGHQAFISRYALGRDYHKVLRGRLQKLVEKMEVAIGKFNYRVFSDSAPVMEVEWASK
                     SGLGWRGKHTLLLSREAGSFFFLAEIYTDIPLPVDEPSKNYCGSCTRCLEVCPTNAIV
                     APYKLDARRCISYLTIELKGSIPETLRPLIGNRVYGCDDCQLICPWNKFAKSTQEPDF
                     AVRNGLDDSSLVALFAWTQDEFETKLAGSPIRRIGYQQWLRNLAVGLGNAPSSPSIIQ
                     ALKDRSNDPSTLVQEHVHWALKQHEAR"
BASE COUNT         4334 a         3193 c         3595 g         4562 t
ORIGIN      
        1 tggcgcgcat cttcttcgtt gattgcttca agctcaagcg ataccgtgcc ctgattggca
       61 agaatggcct tgactgtgta acgcattaac taaaatatta ccaatttgcg atatcagccg
      121 cgtcccctgt tccgccgggt tgaccatctt ttcctagaga aaacaaatcg aattctgcac
      181 gatcacctgg atatttatac tgataagggc gtccccaagg atcaggtggc ggcattttgc
      241 ttaaataagg tccttgccat ttcaattcgt tgcccgggcg ggtaactaac gcattgagtc
      301 cttgctcact cgtgggatac tgaccaacat ctaagcgata ttgatgcagc gctttttcca
      361 gcgaatcaat ttgagcctga gccatcttga cttcagattt gcccacttgt gaaaaatatt
      421 tcggcccaac ataagctgct agtaatccaa taatcaccat aacaaccaaa agctcaagca
      481 aggtaaaccc ttgtattgtg cgtttgccta aattcgtata acagcctttc atgcttatcc
      541 tcgacgtata aaactttcct aataacccat aaatatcagt agattcaagg cccatcgcaa
      601 attaagcaac actaaacttt ttcacgactt tttacttggt cttgacaatc ttttttttac
      661 gataacatat taatctcgcg aattccaacc gctcaaatcg gctaaccgtg cgatttgtca
      721 ttatcaccaa acagaatctt ggggcttgaa tcaacggcgt tccatttctt tttttaatta
      781 tagtattgat attagacatt tttattaagt taacgatctg cccgatcgat gtgatcgttc
      841 aaaggtaaca ctgatcagta gttcgctacg gtgcgacaat atgtcacatt gattgttctg
      901 attacgactc gttattattg ccgaatccaa actacaaagc ccctgctctg agggcgtctt
      961 tgtcctacga aaacgaataa caataataat ttgagagaaa atcatcacga atggtggatt
     1021 tatcagatat cgatttaact tataagttag taattggatt atttaatgca gccccaggta
     1081 aggcaaatct ggcaagtttg ctcagcgcaa ttgacgacgg attatcattg cctcaaattg
     1141 gcgatcgatt agattcgaca ttactattta accaaagaat cattggcgat ttatctgagg
     1201 ctgatcaagt ttctttgatc atgagtcact ttggattcgt tcatggccaa tcaaccggca
     1261 atgaaagaaa acaggtcaga gattacctca ctggccgctt gaaatcgggg gatagttggg
     1321 ggcagatcgt ttatgacgca gttgtctact tgtccggtaa tccggatcca atgtttgcca
     1381 aagctgcttt gttgctcagc aacaaggtgc tagtttcaag ccttttttca caatcttatt
     1441 ctgaagatag cttagaagtg ctacaaagcg tgctatcggg tgtatctgcg gacagcctat
     1501 tagacgaagt atcagccgag gcatatctag ccgaaatcgg taaaccagtc ggtgctgtca
     1561 acctgacggt agcaaaggat gtgctcagta gtcatgtcat actcgctcca aggggctata
     1621 cgcctgcagg aacggatcaa atcaatacat tgaatgacga cgatgtgtta tccggaaccg
     1681 cttccgaaat tgataagcta gtatttgatt ttgtcaatga tgcggatacg ggcgatcata
     1741 atatcgtgcc gcagttaagt ggtatcgaaa ttctcgacat aaagttctcg cttgatggcg
     1801 caggaacgct tgatttacaa gataccgttg gtgttactca tatcgatttg caccgcatcg
     1861 atgatggtgc taccgcgatt attgataata ttaccgaagc aacgacgaat catttatcca
     1921 ttaaccgttc aaatgttacc gattcgaaca ttgcgtttac ttatcttgat tctgcactca
     1981 tcggtactga tgatgtcgta acgttaacac ttaacaatgc ccaagctggc gtggtctcaa
     2041 ttcaagaatc gactgatgat ggttctttgg atcagggttt tgaaacgatt aacgtgatct
     2101 cttccggaga tcaagctaat acactgatta gtttgacgac tgaggatgcg caactactca
     2161 acattacagg taaccaggac ctgactattc aagcattgag cggaactaac caatcctcat
     2221 tggtggggct tcaaaatatg ggcgggtcgc tcaccaaaat cgatgcatca acattcgcag
     2281 gaaatttgag aatcaatatt aatggtttgc tggatgcggt cttggatgat tcttcgggta
     2341 aagatgtgcc agttacagta ctcggtggaa taggaaatga tacgatttgg ttgagcagta
     2401 aaaacaatgt caatgatgaa ttaaatggag gaactggggt taataccctg attctagggg
     2461 gcggttttga ttcggcaaca gtaaccgagc ttaacccgga taagctaaaa aatattgtta
     2521 attttagtac cttggcgagc actaattttt ccctaacgcc tgagctttta aaattgcagc
     2581 tcgatgattt tgtgctggat tcattggttg acagcgaaca tatcagtagt atcgaggagc
     2641 cagagaccct taccctacgc atcaatgatt ttcaggatat tgaaacacct caagctgcgg
     2701 ttgctttgca gttagatatt tcagatttaa cccagcaatc ggcggtcaac gtgacgcttg
     2761 atacaggatc gccccactat cagctcgaac acgatacgat tcagttatcg ccacacggaa
     2821 gcagtattac catcaataac ttccagacgc tttccgacgg ggcggtcgtc gatgtttttg
     2881 gtggatctgc cgcagatcaa atcattttat cgcttgctga ttttaagttg gtagattcga
     2941 gtgctggcaa taacattgca atcaccgatt ttttatttgc gttcgatact atcgaccccg
     3001 cctcagatcc ctctgtcgaa gccattatct ttgattcaac gacaggcaat ctcttttata
     3061 accctgacta tgcaaccgaa ggcgggttag tgctgattgc tagtttaatg aatatctctg
     3121 atttgactga tctcgatatt tttattcttt cttaggcaga aagttcccaa aaaatatgct
     3181 tagttaataa ccataatgcg tacatgccta tgggtgcgac aatatgtcac attgttttct
     3241 aatggatatt agaagatact gccagcgact atcgatagtt cattaataag taaaaagttt
     3301 agccttatca aaaatttgaa cttcaaagag gagtaatcaa tgcagaaaaa tagctcatca
     3361 aaaaatatga gtcgtgcact tatcgcaatg ggactaatca tcgcagcgag tcaagtgtca
     3421 gcgcatacac gcttacaagt cccacaaatt cctgaaggaa cgcgagttta caacaatgtt
     3481 gtcgttggtc acggttgtga agggaaagat gtcattgcaa caacggcagt gtttcctgac
     3541 ggcgttgatt caacggtgtt ggttgatggc aacccttatg aaggatcgat tagcgaattc
     3601 atcaactggg gtaatctgat tcagaaggtg atgagtaaag atgtttttgc taaagaagcc
     3661 gaaaaatatg atcaatcagg cagtaatgtc gtcggtttct gggttagaga tggcgcgctg
     3721 cccgcaacct atacaggatt atttcctttc agatccgatg cggcagtaat tgaaccttct
     3781 tcttgcgcga agagcgtaaa agttgcagta gccattgcag atatttgcaa aaacaccaat
     3841 atcggtggtt ttactgatct gaccgttaat ctttggacgc ctgcagtggg ctcaaacttc
     3901 gatggcccgg gtttgcatgg ctataactca cctgcaacgc taaccattaa tcgtgattta
     3961 gcggcaaatc cgctcgatgc atcgtgtgga gaaggttcag ccgttgaatt gctgccatcg
     4021 gctgcacagc ttgaccgtga catgccaatc aagaataaag atggtggtgt ttggtggcca
     4081 aaaaactaat ttgcgagtta gtttattgtt tttaacgtaa acccatcgtt tacgtagcag
     4141 ttcaaccata acgcacaagc gggcagttac attgtagttt gatgtgattg ctcgtttaac
     4201 aggatgttgt aaggagggca ggatgctatt ttcgaagcgt aacaaagcac aatggatagt
     4261 tttgattccc tggttcatcg cggcccaaat gagcgccgct tcagcttccg agcaagattt
     4321 aagtagagaa actaaagctc atgctgcttc ggcagcccgc attcattgca gtgataacag
     4381 tgcttatcaa cgtgaacccg atcctgagct caccgcttcg attaaatcaa tggcggaatc
     4441 aacggattca tcagaaaggt tggttgcact tgcgcaattg tcggaacaac gggctatcga
     4501 tcctgaagtg gctgatcaaa ttttgtatat tgctttattg gacgatgatc cgatcattcg
     4561 cggtcaggca gtttatgcaa ttggtcggcg cggtggtatg gatgccagca tcgtcatcga
     4621 tcaggcactc gcggatgagg ttccttccgt cagattgatg gcagtcgatg cactagctgc
     4681 gagtgatgaa gagagtaatg cgctgctcag gaaggccttg gaggatgaaa gtgcagcagt
     4741 gagagagctg gcatcgacca agctggggtt agtcgctaag taaaacgaaa aagatagcag
     4801 tagatggcac atcattgata ctaattaatg atgtttttat ggtgaaaatt tataggagag
     4861 gactatgaag atttttaaaa ataaatttgt actcattagc agtttattag gcgcagcatt
     4921 tatattttca ggaaatgttg ctgcacataa tcaagccggg agttttacta ctggccttgc
     4981 aaatgcggtt gatttttatc aagtaacttg ttcagatgat ggcagcggcg cgccttctta
     5041 ccttgagttt caagtaaagg atacgacagc caacacagct aaagtgcagg cgctcgttca
     5101 gaaaggtacc tcttgcggtg tcaatgcctg tgcagttaca acgctcgata cgatcgatac
     5161 ggatgcgctc tatagtcctt tagctcaggt aaagcagggt gcgggtgttt ataatctatt
     5221 tgtgagtcac acgagcacag gggctgatgc ctatgatgta ttgctccact gtaaaacggc
     5281 agcaggcgtt catactggta cttcagttgt ttcaagacag caaaaataat tagcactaac
     5341 tcaatttacc attcttcccc tcagtcgctg cactgcaaac tggtagttat agagcgcagt
     5401 tgcagcgatt ccgagcgctc atctctgttt tcccacgaat ggcgcgttga gtagtttttc
     5461 aaatattttc gaataaactt tgcatcttat ttttatacaa tgccggcatt gttggagtaa
     5521 ataagattta atttctatgg tgaattaaaa aatgaataaa cattttagaa tcaatcagct
     5581 tttccaaaca tttttgatga taggaattct gatccatttc tcccagagtg tactcgcaca
     5641 tacacttttt gatattcctc gtttgcccga gttaaagaaa acgactaacc atatcacgat
     5701 tggccatggt tgccagggta acaccgtcat tgggactagc gtcgtgttcc ctgatgggca
     5761 agattccact ataacgatcg atggcaatgc ccatagcgga acactggatg agtttgtctc
     5821 gaactggggg gggatatttc agttacttca agatcgttct gtgtttgcag aacaagatgt
     5881 taagcaagat ggaaacggta ataccactgg tttctggtct ggcggtgggc gtggcatggc
     5941 agcacatttg aacgcgcaag ttccttttac gatgagcgca gttttgttga attccgaatc
     6001 ttgtgcaaaa agtgtcagat ttgcggtggc agtggttgat atttgtacga ttacgacttt
     6061 tgctgatttt gcagatgata cggttggttt atgggttcca gcagtgggtt cccagttcga
     6121 tggtaacccg ggtggccatg catatgattc acctgcttat ctcaccattg ttcgtgattt
     6181 agagggtaat ccattgccag aatcctgtgg tgaagggatt gaagaagttc tcgtcaaacc
     6241 atcggcggct caaattaatc gtgacatgcc cattcgcttt aatgggcaac aagtctggcc
     6301 acaaaattaa aaaagcttag gtttgttttt ttgtaatccg aattcctgaa gataaattcc
     6361 aaatcaatag agtcattgac aagcgtaatg ggcttgagta cattgatttc tgttctatcg
     6421 aaaaataaat ttttcctaga tcagtttgcg agacgttttg ttactaaaat tatggagaat
     6481 aaattatggt aagacggaca ctcactgcat tggcagcctc attattgctt cttcccgttt
     6541 cggttctggc tgcaggatat ggcccggcag gttgtggtct aggttcgatg gtcattggct
     6601 ctaaaccagg gatggaacaa gtgtttgcgg caacgacaaa cggaacatct ggttctcaaa
     6661 cctttggaat tacttcaggt acctcgaact gtggcgatca tggccttgtt aatctgagca
     6721 aggaacgtga agtgtttgct caggaaaatt atacgagctt agtcaaagaa atggcgcaag
     6781 gtaaggggga aagcctctca accttagcga gcttgtatca atgccctgca acgacacatg
     6841 gtgagtttgg gacgctagtt caggaaaaat tcgattattt agttgcgaat gaccaaacta
     6901 cttcagccga attattgagc aagctcgatg gtcagttgat gcagcatccc aatttagcga
     6961 attcttgcaa tatcaaatta tccgcactta attaatcatg ttacggtaga tatgaatttg
     7021 tttcatatct accgattcag tgggtgttga gcattacttt gtgtcgtatc gtattctcag
     7081 ttttggttta gttttctgtt tttttttatt ttctcatctt gcactagcga ataatgaaga
     7141 agctctccgt tatttgatag agcttcaaca acaagcacag cagaaaaagc tagcacaagc
     7201 ccgtgtttgg catcgattac tcaaatacaa tccgcaactc ttacgtggtg ttatgagtga
     7261 ggcggatggg ctggattttt ttaatgcgcc ccaaggaaaa atagattctg acgctgaatt
     7321 agcagcaacc ctcgcaagtt tttttttacc gctttctgac gttcctgaag gtaaggagca
     7381 tcctcaatgt aacttccccg cccgttttaa atggctcaat cagcaacttt catttgatcc
     7441 acaacgactc gacatacaaa gctgcgaccg tctcgatcgt tggttgggtg tattagatcc
     7501 cgtgggggtt actttagttt ttgcttccta ttttatgaat aacccggcct cgatgtttgg
     7561 tcatacactg atccgcattg atagccggag aacaggcccc gataatcact tgatcaatta
     7621 tggcgcaaac tatgcagcga taccggatac ggataatgct tttttatatg cattaaaagg
     7681 tctgattggc tcattccaag ggcgttttgc gatttttcct tattacacca aagtgcaaga
     7741 gtacaacaat tgggaaagtc gtgatctttg ggaatatgaa cttcagttta atgaagatca
     7801 actacacatg ctgttgttac acttgtggga actggggggc acttattttg attattatta
     7861 ttttcaggag aactgttctt atcatgtgct ctcattgctt gaaattgcca atccggattt
     7921 atttctgaaa gattcgttct tttttagtgt tattccgaca gatacggtta aagtggtttt
     7981 ggcccaggaa ggtctagtca aacaagtcgt ttatcgaccg gctgtattga atttgatgaa
     8041 cgataagcgc ctaagaatga cgtctgatga gcgaaagctt ttcgaagcgt tgatcaaagg
     8101 taacataact atcgagaacc cctcgtttac agagcttccc gttcggtcac aagcgaatct
     8161 gctcaacgcc tacatggatt atcgccagta ctacagtatg caagaagata gcaaccatga
     8221 aatcaagatt ccacgcgcag ttctattagc taggagtcga ttgccaccga ctgaagacaa
     8281 tgatcaaacc attcgttttt cgagtccacc ccatcaaggg catggcacag atcgctttcg
     8341 tttaggctgg ggacacaatg atcgtgagcc attcgtagag tttgcctatc ggcccgcata
     8401 ccatgatttc atggcaaaaa atgtgggtta tgataaaaac tctgaaatca tttttatgga
     8461 cttcaagctt cggtactacc ttgagtcaga acgcgtcaga ttggatcagg ctaaactctt
     8521 gtcgattact tcgtttaatc cctatgaccc gctatttgca cgtacatcct ggcgtgggga
     8581 aattgagatt gacacgctga gagatcggga ttgccattat tgcaattctt tcaaagggag
     8641 ttatggaaga ggcatggctt atcgccccca gtttttctcc ccactattat tattctcttt
     8701 tattgatgtt aaaactgaat tttcaccgca tctaaatcaa aattatcgct tgggtggcga
     8761 tgttgaggtg ggcgcatact atgatattac cgataattta cgcgttaaac ttgcagggag
     8821 ttatcaggtt tttttcttag gagaaagcaa acgtttcttt acagcgcagt ttctaactcg
     8881 atatgcgatt gcgcaagatc tcgatatcag aatggaattg aaccgttacg accataacca
     8941 cgaaggaatt ttcgctatca attatttttt ctgactgatt gatttaaatc gagctgggtt
     9001 aggaaattta gaaacactgt acgatatttc ttctcatgct gccagaaaat atcagtatgg
     9061 tttccattgg gaatacgcca tatcgatttg ggttcagaga gcttttcata aatggcttca
     9121 ccgaattggg gttcgacaac gttgtcttgt tgtccatgga tgacgagcac ggggactggc
     9181 gcaattgttt ccaaatgagt gggtgcccag cgatcgctga gcaataaata actcaacggt
     9241 tgcaatggcc aagtaaacca gtgttgcgac agtttatgac gcgcaatctg ttggaatgaa
     9301 tcaaaagtac tctctgcgac cactagcttt aagtttatat cttcccgctt atcgatgact
     9361 gttcgtaacg cgatgatgcc gcccatgctt tgtccataga caatcatggg aagattttca
     9421 ggatcatggc taagtaccca atcgagtgcc gcatgacccg cttctacaca gctttgcggt
     9481 gtcggttttc cttcggacaa tccataaccg gggtaatcaa aaataaataa gttatagcct
     9541 tcagccgtga tccaggcgag atgcatgaaa tgcgaggtca aattttctgc attgccatga
     9601 aaaaaaataa cggttgcttt cgcaggcgtg atggtcgagg ggaaccacca gccatgaagt
     9661 tttcgttgag cggcatcggt aaaatagatg tcagtagggg aaaatccaac tgacttaggg
     9721 tcatagtaaa gtcgattctg actaggataa tagagaacac tggagcaact ggtcagtaac
     9781 catgtactca gtaacatgag taaacgaaat gctttcaatt taagtatcag cgtagccttt
     9841 tgcgctaata atgggttatc agaataagag tccgcgagtt cagaacataa cacaattacg
     9901 cagtttcttg aggcgttttg ctaatggcat taccttccag attaatatgt tctatgggga
     9961 attatatgta gagcgttcgg tggcgttgta tctattcgcg attgcttgca ggtggccaga
    10021 tcggtaccgt agatgcgcga catctggatt gaattttaac cgccaactgg agcgattgaa
    10081 gttagtctga tgatatttgt gctactggat agagttgtga cggagaacgg caagcagggc
    10141 taataccaag cttgccgttt gttcatgaat caattaactg attttcttga tgctgcgacg
    10201 agtgaaagcc aaactaacaa gaccaagacc aagaagtgca accgaagtgg gttcaggaag
    10261 ttctgaaatc acaatatcga atgcccaagc acttgttcgt gtatttccat cggcatcgat
    10321 aaacccggtt actcccgacc cggtaaatcc gtctgccaga ttcgggccaa ttgcaaaatt
    10381 ggcatactgt gtcaaagatg ctgtgtagtt tcctatatcg agaaaaatgc taagcacggc
    10441 atcaaattca gcgccagtaa caggatcggc tgctactcct gcgccatcat cattatcatc
    10501 gatcaaatta cctgtgccat taaaaagcgt aataatagga tcgaagccac ccgcagatat
    10561 agtatttccc tcagcatttg ttccacctgc gtaagaccat gtgcgaagtg taacaagtga
    10621 tggttcagtc acagcaaaac tgaagaactc tacgtcatca tcagtgttaa agctaccagt
    10681 aaacgaagaa ctgataggag cagcgccaac ttgagcaact gataaagcga atactataca
    10741 tacagcaaga aacatttttt tcatttaaat ctccaaatat ggttaaaaag ctgcagattt
    10801 aaccataacg cagttatttc taaagcaaat tttatgccat tctaattact tgttgattaa
    10861 caaatgctta agcttatttt cgatagatga atcactagca gtgtaaatta atccgacagt
    10921 aactaacgat taggcatcaa ttatttggaa aatgaattat gtttttatta gaagaagcat
    10981 gttatagagg tataaatgat ctcacggccc aaaagtaaaa tgttgcatat gagagattca
    11041 ggcaatgagc atagcaagtg taaaaaatat tgacatcttg gtataaatgc gcagaaannn
    11101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngca tgatatcgtg atggtcttaa
    11221 cattgaaagc gttagcaacc tgcactatga ttcggtgtta tttaaggata tggaaataaa
    11281 atgatcaatt ggtcacaaat tctgatgttg gcaaaattgg gtaatccccc tccatctcag
    11341 cgcgtagaaa agacagaagc tgaatggaag tcgctgctca ccgatgagca atatttcgtt
    11401 ttacgccaga aaggaaccga gccccctcac agttcaaaat tatgtacgca attcgagcct
    11461 ggtttatatg cctgtgcagg ttgcggcacg gaattattcg atgcatcatc caagtttaac
    11521 agtggaacag gatggcctag ttttacgcaa ccggttgcag agaatgtagt ggcttattat
    11581 cttgatacca gttatggcat gcagcgtata gaagtgaatt gtaatatttg cggtgggcat
    11641 ttgggccatg cttttcctga tggtccagaa ccgagtggac taaggttttg tattaacgcg
    11701 ctttcgctca agaaagtata actggcttta aggaacatca atttctcatg acgctgcagc
    11761 ataatccctt gatagcatct caattaccga ttcgactcgt tattcatggc ggtgctggtg
    11821 ctatttcgcg tgctcgcttt acaaaagagc gtgagcaaac ttatcagcag acacttcgag
    11881 aggcattact cgcaggctat gctgtgttga atgccggtgg aagtagtatt gaagcagtga
    11941 ccgaagcaat tgtcgtcatg gaagattctc cactctttaa tgcagggaaa gggtcggttt
    12001 ttacccatga aggccataat gaactagatg ctgcgattat ggatggcgct aaccgtatgg
    12061 ctggtgcggt agcaggggtt aaaacgattc gtaatcccat tcgtgctgca cgagcggtta
    12121 tgactaaaag caaccatgtc atgctgattg ctgaaggggc ggaagctttt gctcgcgatc
    12181 aaaggctaga gatcgttgac gcgacttatt tctatactca gcaacgctgg gagcaattgc
    12241 aaagaacgat tgagagagag cgtgtgcagc accaacaggc aatagaagct gattcgttgt
    12301 tggttgagaa agatgataaa tatggaacgg tcggtgcagt tgcgcttgat cgcagtggaa
    12361 atttagcggc agggacttcg actggaggac ttgtcaataa gcgttatgga cgtgttggcg
    12421 attcaccgat tattggcgct ggtacttatg cggataatcg atcagtggct gtttcgggca
    12481 cgggtactgg ggagatgttc atacgaaccg ctgcagcaca tagcattgca acacgagtgc
    12541 agctaaagca actatcaatt accgaagccg cggatcaaat actagccgaa attgctgcaa
    12601 tcggtggtga tggtggggtc atcgtgctcg ataagagtgg tcattacacc atgcgtttta
    12661 taacttcagg tatgtaccgg gggacaatcg gcggggacgg agtgccttgg gtaggaattt
    12721 ttccagaagc ggagattgct ttgaatgctg aaaaatagtc gtggatcgat ttggagaatc
    12781 aatccttcga agcgtgattc ttcctaattt acctttgcta attcagtcct ggctggaatt
    12841 gcgcggctat tactaaaata gcgtttgatt ccggaaacaa tcgcgctagc cattctatct
    12901 tgatagtttt cgctttttaa tttctcttct tcagttggat tactgataaa tgcagtttca
    12961 actagaatgg atggaatatc cggagattta agcactgcaa accctgcttg ctctacttgg
    13021 cgtttatgga gatgattaac gttgcctatc gctgaaagca cggtaccagc aagtttaata
    13081 ctgtcattaa tggtggcatt gatcgataaa tcaatgagtg tttttctaag gtaatggtct
    13141 ttagtattta acttaattcc accgatccgg tcagattcat tttctttttg cgctagccag
    13201 cttgcggtag ttgaagtggc accattttca gataatgtat aaacggaaga tccacgcgca
    13261 tcattcctag gaactgcgtc ggcatggaca gaaatgaaca agtctgcatt tgcactgcgg
    13321 gcctttgtgc gacgaactgc aagcggtaag aaataatccc cggtacgcgt gagtaccgcg
    13381 tgcatacccg gttctttatc aattttagct tttagcttct tggcaattga aagcgtaata
    13441 tctttttctt gtgaacctct gggcccaatt gcccctggat cttttccacc atgacctgca
    13501 tcaatggcaa caatgattgt tttttgtttt tccggtctag gattgaagct ggctaaggtt
    13561 atttctggtt tattagcgtt tttagcttta gtcgctattt tttcctctga tgctttaaca
    13621 agtgccatta acggatcatc gtggtgccca tttggttttg tcgcagcgcg atccggtgga
    13681 aatatatcga gaaccagacg atagccgaat ttgtccatcg ggtccaaggt gaaggttcta
    13741 ggcacagcct tcgttttaag tgctaaggtt aaccgaaccc catttgaaga taaatattca
    13801 atgtgagcct tctttattaa aggatctttg gcatcaattt ttgtagggag acttcctagt
    13861 ggtggattta ggccggtatt ctcaagatcg agtacgattt gatcagggct gctttgtaag
    13921 ctaagcgtat acttaatcgg tagattggat tcaagggtta cccgggtata ttcaggcccg
    13981 gcccaaactc gcgtggaagt aacttgcgca ctcgaatttg catgcactgt aaatgtcaac
    14041 acgcttaaac acaatagaac aagccctgcc agcataaact ggtagttctg gccaacgcgc
    14101 ggttttttta tcatgctagc aatgctaggc attttttacc ctgagtggta ttcgcgcaaa
    14161 ttccagcgaa tcgacccctg ttggcaaatt gaagttttat cgttaaatct gctgttggca
    14221 ataaatctcc ccctttttct ggccattcga ctaaacagat cgtttcagaa ttaaagtaat
    14281 cacggaaacc agctgcttcc cattccgaat aatcattgaa tctataaaaa tcaaagtgat
    14341 acaagtataa cctagaaaaa ttatagattt caaccaaatt atatgtcggg cttttgactt
    14401 ttccttgata acccatgcca cgcagtattc cccgtgttag cgctgttttt ccagcaccga
    14461 ggtcaccctt taaaaaaatg attaagccgg catgaagcgt agttgcgatt tttgcgccga
    14521 atgctaaagt tgcagcttcg tcaattaaat gtaagcaata ttgctgctcc tcatttggca
    14581 cagcctctga acaatcacga ttatgcaaga agaaatctcc cagaatggaa cagttgatcc
    14641 ggtttcactc aaacaatcga taaccaattg ggcccatcaa ctgggttttc aagatatccg
    14701 gatcgccaat gctgaggcgg atatgtcatc agttgaaaca gggctgtcta catggttagc
    14761 gatgggtttc catggtgata tggattatat ggcaaaacac ggcattaagc gcacacgacc
    14821 gaatgaactg gtccctggta catcgcgggt tatttcggta cgaatgaatt atttgccgag
    14881 tgacgtgaaa gagagttgga gtgtgatcga agatggccat caagcgttta tttcccgtta
    14941 tgctctcggg cgggattacc ataaagtgct gcggggtcgt ttgcaaaaac tcgttgaaaa
    15001 aatggaagta gcgatcggca aatttaatta tcgcgtgttt tccgatagcg ctccggtcat
    15061 ggaagtagaa tgggcatcaa aatcagggct tggatggcgt ggtaagcata cattgctctt
    15121 gtctcgcgaa gcggggtctt ttttttttct agcagaaatt tatacggata taccgctccc
    15181 tgttgatgag ccgagtaaga attattgcgg gagttgtaca cgctgcttgg aagtatgccc
    15241 gaccaatgca atcgttgctc cttataaatt agatgcacgg cgttgtattt cttatctcac
    15301 gattgaactc aaaggcagta tccccgaaac tttgcgtcct ttgattggta atcgggttta
    15361 tggctgtgat gactgccaat tgatttgtcc ctggaataaa tttgctaagt ctacccaaga
    15421 gcctgatttt gcagtccgga atggcctgga cgatagttcg ctggtggcat tatttgcgtg
    15481 gactcaagat gagtttgaaa cgaagcttgc tggtagccca attcggcgca tcggttacca
    15541 gcagtggctc agaaacctgg cagtagggct tggaaacgca ccgagttcac cctccattat
    15601 tcaagcgcta aaggatcgca gtaacgatcc ctcaacgcta gttcaagaac atgttcattg
    15661 ggcacttaag caacatgagg ctaggtagcc catttacaat atgcaataac tatcgcatgc
    15721 gccatgcgtt atatgaagtt tcgcgatcaa aatcatccca aataaaggta ggattgggaa
    15781 tggg
//
LOCUS       DAOQKQ010000046        15773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_1715_length_15773_cov_5.802583, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000046 DAOQKQ010000000
VERSION     DAOQKQ010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 15773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15773
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1715_length_15773_cov_5.802583"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1366)
                     /gene="hypF"
                     /locus_tag="PKZ49_04520"
     CDS             complement(<1..1366)
                     /gene="hypF"
                     /locus_tag="PKZ49_04520"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HND35860.1"
                     /translation="MSILTRNEIIRRRYALKGAVQGVGFRPFVYHLANELGLKGWVRN
                     DSAGLIIEVEGDETKIADFEKQLQDHKPLNSIVSHIESFDLTPQLEVEFRILSSDSNP
                     EITTLILPDLAICQDCIREMNDTADRRYRYPFINCTQCGPRFSITTALPYDRPNTSMA
                     AFEMCPECEREYQDPLSRRFHAQPIACAHCGPQLELWDQQGNSVAAGESALSQAIMQI
                     EQGNIIALKGLGGFHLVADATHDHAVKTLRARKHRKTKPFAVMYSSLEAIQADCVMAA
                     EEKALLCSAAAPIVLVLRKPGAAFSPWIAPDNPYLGVMLPYTPLHYLLLQALQRPIIA
                     TSGNRASEPICIDNQEALEQLRGYADYFLVHNRPIINRADDSIARIMAGQTTLLRRSR
                     GYAPLPIELNEATQQPILAVGGHLKNTVAYMQGGQLMLSSHIGDLSTSKAMAAHEQAI
                     VAFTD"
     gene            complement(1344..1610)
                     /locus_tag="PKZ49_04525"
     CDS             complement(1344..1610)
                     /locus_tag="PKZ49_04525"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HND35861.1"
                     /translation="MHEFSLMADLFRKIREISNDNNNRKIVTVRVKLGALSHITPHHF
                     EEHFIDFSKGTPADGAKLEIVQLDDRNDPNAQSILLESVDLDAQ"
     gene            complement(1603..2082)
                     /locus_tag="PKZ49_04530"
     CDS             complement(1603..2082)
                     /locus_tag="PKZ49_04530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HND35862.1"
                     /translation="MTAKILIIGIGNRYRGDDGLGCFIVDELKKQVTHDMEVIEHNGD
                     PASLIDLWQGRSQVILIDAVSSGAEIGTIHYVDLRQQALPDTFQSYSTHGLGVAEAVE
                     LARVLGKLPPHIVFFGIEGENFETDAQQPVRLQQAITALQDRIMKAINTEIGESQRA"
     gene            complement(2079..3383)
                     /locus_tag="PKZ49_04535"
     CDS             complement(2079..3383)
                     /locus_tag="PKZ49_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HND35863.1"
                     /translation="MDQENKTRTIKVDYLARVEGEGALYLKIQEGKLEEVQLKIFEPP
                     RFFEGFLRDRHFSEAPDITARICGICPIAYQMSAVHAIENAFNITIGGALRDLRRLVY
                     CGEWISSHALHIFMLHAPDFLGYESAIHMAKDFPEKVEQGLQLKKVGGMLMSLVGGRE
                     IHPINIRVGGFYRAPSKQELQTLVPALKQAREDALETVRWVSSFDFPDFEQHYTFISL
                     RHENEYPINEGRIVSNHGLTISASAFDDYFEEIHVAHTNALHCVLKADHHPYHVGPMA
                     RYALNFDRLPEPIQWKAKQAGLENVCRNPFKSIIVRAIEVLYACEEALRIIDTYTAPA
                     VSFVEIEPKPATGYAVTEAPRGILYHRYQIDGEGDIVDCDIIPPTSQNQKMIEEDLKQ
                     FVPRYLKLSDERLQWYAEQAIRNYDPCISCATHFLKLNVDRS"
     gene            complement(3367..4155)
                     /locus_tag="PKZ49_04540"
     CDS             complement(3367..4155)
                     /locus_tag="PKZ49_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfhydrogenase subunit delta"
                     /protein_id="HND35864.1"
                     /translation="MEPIEKKKPKLAVWKFASCDGCQLTLLNCEDELLTLTENIQIAN
                     FVEASREVLPGPYDLSLVEGSITTPQDLTRIQQIRAQSNYLVVIGACATAGGIQALRN
                     FKDVKEFINIVYAHPSYIDTLATSTAIAEHVNVDFELRGCPINKQQLLEVINAFLHRR
                     KPNIPSYSVCIECKRRGNPCVMVMGKPCLGPVTHAGCGAICPSYQRGCYGCFGPMETP
                     NMSALGDWLLQKGMSQKDWVRSLRAFNANSPAFKEESHKHGSRE"
     gene            complement(4206..5039)
                     /locus_tag="PKZ49_04545"
     CDS             complement(4206..5039)
                     /locus_tag="PKZ49_04545"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HND35865.1"
                     /translation="MLIQALLNNPMEPTPFRILRVKQELKDCFTLILDCKQTPFHFAP
                     GQFNMLYIFGHGEVPISMSGDPNQPELLVHTIRNVGSITAALEQLREGDFVGVRGPFG
                     SPWPLSEAKGKDIVILAGGLGLAPLRPVIYHTLENQRDYKKITLCYGARNPEAILFSD
                     ELEQWTHRIAVELTVDSAGPGWSGHVGVITDRLQQQAIVLTNTIAFVCGPEIMMRYSA
                     YALIDNGLSADRIYISMERNMKCAMGLCGRCQFGPYFICQDGPVFSFDKVEQLLKIRE
                     A"
     gene            complement(5044..5517)
                     /locus_tag="PKZ49_04550"
     CDS             complement(5044..5517)
                     /locus_tag="PKZ49_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HND35866.1"
                     /translation="MVKIRNIEALLLESPFFQSFPQDHLRLLAECSEIVHFNENELLL
                     TEGEKADYFYLILSGSVAIESHYPGREALIVSKVVTQGIVGFSWLFPPFRNQFDARAA
                     TRGSAIKLNGSCLLPIIESDHALGYYLLKGFSAIMLQRMQSARRQMLDVFGQSKN"
     gene            complement(5504..6673)
                     /locus_tag="PKZ49_04555"
     CDS             complement(5504..6673)
                     /locus_tag="PKZ49_04555"
                     /inference="COORDINATES: protein motif:HMM:NF028488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HND35867.1"
                     /translation="MSLREISKSNFFQKVKIDRAHIDVLFQSLKNRGYRLVGPKISEG
                     AIIYDFLDVVDELPIGWTDEQEAGTYRIKQREDQALFGYNLGPHSWKKFLFSARKRVW
                     QGQKTSEGSFKTVQEMVAQSQPYAFIGVRSCELHAIQIQDRVFMNGQYQDTEYTQKRA
                     DNFIVAVNCGQAGKTCFCTSLNTGPRVSQGYDLALTEILDGGTHYFVVESGSERGLAV
                     LQELPCQSVGNNDLEAVDKCIEKAVGQMGRAIDTTDIKAFLYRNYDNKHWRKLEERCL
                     TCANCTMVCPTCFCSKIEDTTDLTGEYAERWQTWDSCFTMDFSYTHGGAVRASTQSRY
                     RQWLVHKLASWIDQFGTSGCVGCGRCITWCPVGIDLTEEIKALKQAEREAKNGEN"
     gene            complement(6974..11206)
                     /gene="rpoC"
                     /locus_tag="PKZ49_04560"
     CDS             complement(6974..11206)
                     /gene="rpoC"
                     /locus_tag="PKZ49_04560"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HND35868.1"
                     /translation="MRALLDLFKQVTQKEEFDSIKIGLASPEKIRSWSYGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTLSKVRRERM
                     GHIELASPVAHIWFLKSLPSRLGLVLDMTLRDIERVLYFEAYVVTDPGMTPLTQKQLL
                     TEEDYLNKTEEYGDDFRAAIGAEGIRDLLNSMNVQVEIDSLRLEMQSTNSETKIKKIA
                     KRLKVLEAFNKSGLKPEWMILTVLPVLPPELRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLELRAPEIIVRNEKRMLQESVDSLLDNGRRGKAMTGANKRPLKSLADMIKGK
                     GGRFRQNLLGKRVDYSGRSVIVVGPQLKLHQCGLPKKMALELFKPFIFNKLEVMGIAS
                     TIKAAKREVENETPVVWDILEEVIREHPVMLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAFNADFDGDQMAVHIPLSLEAQMECRTLMMSTNNILSPANGEPIIVPSQDIVL
                     GLYYMTREKMYAQGDGMFFQDMVEALRAYENGAVELNAKIQVRIKEIDMVSKAEKIQR
                     YETTVGRALLSEVLPEGLSFSLVNKVLKKKEISKLINACFRLCGLRKTVIFADKLMNL
                     GFTYATRGGISICADDLRIPVQKNDIIKSAEAEVQEIATQYTSGLVTQGERYNKVVDI
                     WGRAGDQVAKAMMDQLSVENIYDSSSNEIKKDDHGKPLMQESFNSIYMMADSGARGSA
                     AQIRQLAGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTAN
                     SGYLTRRLVDVTQDLVITENDCGTEDGVVMKALIEGGEVIEALKERLLGRVTVQEVIN
                     PDNQTVLYPAGFLLDENAVEQIEFYGVDEVKVRTPLTCETRYGLCAKCYGRDLGRGSL
                     VDVGEAVGVIAAQSIGEPGTQLTMRTFHIGGAASRTAVASQIESKSNGVIQYSNSIRY
                     VKNSRNELIIISRSGEVIISDDNGREKERHKIPYGATLLVHDGDAVKIGKVLASWDPH
                     TRPIITEYSGKVRFENVEEGVTVAKQIDDITGLSTLVVIDPKRRSTSSQSKGLRPLVK
                     FLDENEKEIKVAGTDVSVSITFQIGSIITVRNGQQVSVGEVLARIPQETSKTRDITGG
                     LPRVAELFEARSPKDAGLLAEVTGVVSFGKDTKGKQRLVITDLDNVSHEYLIPKDKHV
                     MAHDGQVVNKGEIIVDGPADPQDILRLQGVEVLARYITDEVQDVYRLQGVKINDKHIE
                     VIVRQMLRRVQITEAGDTSFIPGEQVERADVLIENEKVLAEGKIPAAYNYILLGITKA
                     SLSTDSFISAASFQETTRVLTEAAIMGKRDELRGLKENVIVGRLIPGGTGLSFHNIRK
                     KQKAKSSTEPNLLAEKSNGEVDNVTPINDNMA"
     gene            complement(11260..15333)
                     /gene="rpoB"
                     /locus_tag="PKZ49_04565"
     CDS             complement(11260..15333)
                     /gene="rpoB"
                     /locus_tag="PKZ49_04565"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HND35869.1"
                     /translation="MDYSFTEKKRIRKSFAKRASVLSIPFLLATQLESYTSFLQANKL
                     ANSRKNIGLQAAFNSIFPIESHSKNARLDFISYNLGHPPFDVKECQQRGLTYASPLRA
                     KVRLTILDKESSKPTVKEVKEQEVYMGEIPLMTETGSFVINGTERVIVSQLHRSPGVF
                     FEHDRGKTHSSGKLLFSARIIPYRGSWLDFEFDSKDYVYFRIDRRRKMPVTTLLKAMG
                     CTPEQILADFFVFDHFHFSKNKILFDLVPERLRGEVASFDIVSSSGDIIIQKDKRITA
                     KHVRDLQQSQIEKIEVPEDFLLGKILAKNIIDQKTGEIIALANDEITETILEKIRVAG
                     IKAVTTLYINDLNQGPYISLTLRTDEVADQFSAQVAIYRMMRPGEPPTEEAVIALFNG
                     LFFSAERYDLSVVGRMKFNRRVGRAELEGLPTLTHQDIIDVIKILVELRNGRGEIDDI
                     DHLGNRRVRSVGELAENQFRAGLARVERAVKERLSQAESENLMPHDLINAKPVSAAAR
                     EFFGSSQLSQFMDQTNPLSEVTHKRRISALGPGGLTRERAGFEVRDVHTTHYGRVCPI
                     ETPEGPNIGLINSLALYAQTNEYGFIETPYRKVVEGTVTDQIVFLSAIEESQYVIAQA
                     NAKLDSDGRFIDEIVSCRHRNEFTLSPKDQIDYVDVAPAQIVSVAASLIPFLEHDDAN
                     RALMGSNMQRQAVPCLRAEKPLVGTGIERVVAEHSGTAVRAIRGGVVDFVDANRIVIR
                     VHDEETLAGDVGVDIYNLIKYTRSNQSTNINQRPLVKIGDVIERNNVIADGASTDMGE
                     LALGQNMLVAFMPWNGYNFEDSILISERVVAEDRFTSIHIEELSVVSRDTKLGTEEIT
                     ADIPNLSERQLSRLDESGIVYIGAEVEVGDVLVGKVTPRGETQLTPEEKLLRAIFGEK
                     ASDVKDTSLRVPSGISGTVIDVQVFTREGVQRDKRSKKIINDELDRYKKDLADQMRIV
                     ETDVFKRLERLLIGKVVTGGPKKLAKGTAITKEYLDSIDLHHWFDIRLADEDISRQLE
                     QMKEGLEQKQKLFDSAFEEKRKKLTQGDELPPGVQKMVKVYVAVKRRLQPGDKMAGRH
                     GNKGVISKIVPIEDMPYMADGTPVDVVLNPLGVPSRMNVGQILEVHLGWASKGLGEKI
                     GALLQQEKRVAKLRPFLETIYNSTGMKEDIASLNDDELIELARNLVGGVPFATPVFDG
                     ATEAEIKAMLELADLPKTGQVTLYDGRTGEAFDRSVTVGYMHVLKLHHLVDDKMHARS
                     TGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVGGRTKVY
                     ESIVKGDHKIDAGMPESFNVLVKEIRSLAMDIDLEQY"
     gene            complement(15507..>15773)
                     /gene="rplL"
                     /locus_tag="PKZ49_04570"
     CDS             complement(15507..>15773)
                     /gene="rplL"
                     /locus_tag="PKZ49_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HND35870.1"
                     /translation="AAVAVAAPAAAAASAAEEQTEFSVILTSAGDNKVNVIKVVRAVT
                     GLGLKEAKDLVDGAPKVVKEGIAKADAEAIRKQFTEAGAACDIK"
BASE COUNT         4509 a         3726 c         2862 g         4676 t
ORIGIN      
        1 ggtcagtaaa ggctacaatt gcctgctcat gggccgccat cgcttttgag gttgagagat
       61 caccaatatg ggaactcagc attaactgac ccccttgcat ataagcgacc gtatttttta
      121 gatgcccacc cacagccaga ataggctgct gcgtcgcttc atttagctca atgggcaaag
      181 gtgcataacc tcgactcctt cgcagtaagg ttgtttgccc tgccatgatg cgtgcaatcg
      241 aatcatcggc ccggtttata atcgggcgat tatggaccag aaaataatct gcataacccc
      301 gaagttgctc gagcgcttcc tgattatcga tacagatagg ttcactcgcc cgattaccac
      361 tggttgcgat aatcgggcgc tgtaatgcct gcaacagcag ataatgcaag ggggtgtaag
      421 gcaacataac ccctagataa ggattatctg gagcgatcca cggtgaaaat gcagcacctg
      481 gtttacgtaa caccaagaca atcggtgccg cagcagaaca taacaacgct ttttcttcgg
      541 cagccataac gcaatcggct tgtattgcct ccaaagaaga atacatcact gcaaatggct
      601 ttgttttgcg atgtttacgc gcgcgaagtg ttttcacggc atgatcatgc gttgcatcag
      661 caacgagatg gaatcctccc agccctttta aggcaatgat gttaccttgt tcgatctgca
      721 tgattgcctg actcaacgca ctttcaccag ctgctacgct attaccctgc tgatcccaaa
      781 gctccagctg tggaccacaa tgggcacaag caatcggttg cgcatggaag cgacggctca
      841 gcggatcctg atattcacgc tcacattccg ggcacatctc aaacgctgcc atgctggtat
      901 tgggacgatc atagggcagt gcagtcgtga tcgagaagcg tggaccacac tgggtacaat
      961 tgataaaagg atatcgatag cgacgatctg cagtatcgtt catttcccga atacaatctt
     1021 ggcaaattgc cagatccgga agaatcagtg tcgtgatttc aggattacta tcacttgaaa
     1081 gaatcctgaa ttcgacttca agctggggcg ttagatcgaa tgattcgata tggctgacta
     1141 tcgaattcag cggtttatgg tcttggagtt gtttttcaaa gtcagcgatt ttagtttcat
     1201 caccttccac ttcgataata agacctgcgc tatcatttct aacccatccc ttcaaaccca
     1261 attcattcgc caggtgatag acaaagggtc gaaatccaac gccctgaact gcgcctttta
     1321 aggcatagcg gcggcgaatg atttcattgc gcgtcaagat cgacactttc tagcaaaata
     1381 gactgtgcat tcggatcatt acgatcgtcg agttgaacga tttctaattt ggcgccatca
     1441 gcaggggtgc cttttgaaaa atcaatgaaa tgttcctcaa aatgatgcgg ggtaatatgt
     1501 gataacgcgc ccagcttcac acgtaccgta acgatcttgc gattattatt atcattagaa
     1561 atctcacgaa tttttcgaaa caaatctgcc atcagcgaaa attcatgcac gctgactctc
     1621 tcctatttca gtgttgattg ctttcattat acgatcttgc agcgctgtta ttgcttgctg
     1681 caatcggaca ggttgctggg catcagtctc aaaattttca ccttcaatac caaaaaaaac
     1741 gatgtgaggt ggtaatttac cgaggactcg cgctaactca accgcttcag caacgcccaa
     1801 tccatgggtc gaataactct ggaacgtatc aggcaacgct tgctgacgca aatcaacata
     1861 atggatggtt ccgatttcag cgcccgaaga aactgcatcg atcaaaatga cctgcgatcg
     1921 tccttgccat aaatcaatca gcgatgcagg atcgccgtta tgttcgatga cttccatatc
     1981 gtgggtaacc tgttttttaa gctcatcgac aataaaacag cctaagccat catcgccccg
     2041 gtaacgattg ccaattccaa tgatgagtat ttttgcagtc atgatcgatc gacgttcaat
     2101 tttaaaaaat gcgttgcaca cgatatgcac ggatcgtaat tacggatcgc ttgttcagca
     2161 taccattgta agcgttcatc ggataacttc agatagcgcg gcacgaactg ctttaaatcc
     2221 tcctcgatca ttttctgatt ctgagacgta ggcggaataa tgtcacaatc tacgatatcc
     2281 ccttcaccat cgatttgata gcgatgataa agaatgcctc tcggtgcttc tgttaccgca
     2341 taaccggttg ctggtttggg ttcaatttca acaaatgaca cagccggcgc tgtataggta
     2401 tcaatgatcc gcaaggcttc ttcacaggca tagagaactt caattgcacg tacgataata
     2461 cttttgaaag gattgcgaca aacattctcc aatcctgctt gtttcgcttt ccattgaata
     2521 ggctctggca aacgatcaaa attcagtgca taacgcgcca tgggtcccac atgataggga
     2581 tgatgatccg cttttaacac acagtgcaac gcattggtat gcgcgacatg aatttcctca
     2641 aaataatcat caaacgctga tgctgaaata gttaggccat gattggagac aattctccct
     2701 tcgtttattg ggtactcgtt ttcatggcgt aatgatataa acgtataatg ctgctcgaaa
     2761 tcgggaaaat caaacgagga gacccatcgc acggtttcaa gtgcatcctc acgcgcctgc
     2821 tttaatgctg gcacgagcgt ttgtaattct tgtttactgg gagcacggta aaaaccaccc
     2881 acccgaatat tgatgggatg aatttccctc ccccccacaa gactcatcag cattccaccc
     2941 acttttttta actgaagtcc ttgctcaact ttttctggaa aatcctttgc catatgaatg
     3001 gcactctcat atcctaaaaa atcaggggca tgaagcataa aaatatgcag tgcatgactg
     3061 gaaatccatt ccccgcaata aaccagtcgg cgcagatcac gcaaagcgcc cccaattgta
     3121 atattgaagg cattctcgat agcatgcact gcactcatct gataggcaat cgggcaaata
     3181 ccgcagattc ttgctgtaat gtcgggtgct tcactaaaat gacggtctcg caagaatcct
     3241 tcaaaaaacc gagggggctc aaaaattttg agttgaactt cttccagctt tccttcttgt
     3301 atcttcagat acagcgcacc ttccccttcc acccgcgcca gataatcgac tttaatggtt
     3361 ctggttttat tctcttgatc catgtttatg gctttcttct ttgaaagcag gtgaatttgc
     3421 gttaaaagca cgaagtgagc gcacccaatc cttctgggac atcccttttt gtaataacca
     3481 gtcgcccagc gcgctcatat tcggtgtttc cattggccca aagcaaccat agcaaccacg
     3541 ctgataagaa ggacagattg caccacaccc tgcgtgagta accggcccaa gacagggctt
     3601 acccatcacc atcacacaag gattccctcg ccgtttgcat tcgatacaaa cactataact
     3661 gggaatattg ggttttcgtc gatgcagaaa tgcattaatt acttccagca attgctgttt
     3721 gttaatgggg caaccacgaa gctcaaaatc gacattgaca tgttccgcaa tggcagtgga
     3781 agtcgccaaa gtatcaatat aggatggatg cgcgtaaacg atattgataa actcctttac
     3841 atctttgaaa ttacgcagcg cttgaattcc gccggctgtt gcacaagcac cgatcaccac
     3901 caagtagttc gactgcgcac gaatttgctg aatccgtgtt aaatcttgcg gtgtagtaat
     3961 tgatccttct actaaggaca aatcataagg tcccggcagc acttcacggg aagcttcaac
     4021 aaaattcgca atttgaatgt tttccgttag tgtgagcaac tcatcttcac aatttagaag
     4081 cgttagttga cacccatcac acgaagcaaa cttccaaaca gcaagttttg gcttcttttt
     4141 ttcaatcggt tccattacat gttttctctg gcgatttaaa ctatcaaaaa accaagcact
     4201 caagcctacg cttctcggat tttaagcagt tgttctactt tatcaaatga gaataccggt
     4261 ccatcctgac agataaaata aggcccaaat tgacagcgac cacagagtcc cattgcacat
     4321 ttcatattac gttccatcga aatatagatg cgatcggcac taagaccgtt atcgattaaa
     4381 gcataggcac tatagcgcat catgatttct gggccacaaa caaaagcgat ggtattcgtt
     4441 aaaacaatcg cttgttgttg caatctatcg gtaatcaccc ctacatgccc cgaccatcct
     4501 ggtccagcgc tatcaaccgt taactccacc gcgattcgat gggtccattg ttcaagctca
     4561 tccgaaaata atatcgcttc gggattacgt gcaccatagc ataacgttat ttttttataa
     4621 tcacgctgat tttccagggt gtgataaata actggccgca aaggtgcaag acccaatcca
     4681 cccgctaaaa taacaatatc ttttccttta gcttcagaaa gtggccatgg actcccaaag
     4741 ggaccccgaa ccccaacaaa atcaccttcc cttagctgtt ctaacgctgc agtaatcgac
     4801 cctacattcc ggatggtatg cacaagtaac tcgggttgat taggatctcc actcatggaa
     4861 atcggaactt caccatggcc aaatatataa agcatattga attgaccggg tgcaaagtga
     4921 aaaggcgttt gtttacaatc caatataagc gtaaaacaat cctttaattc ttgctttact
     4981 cgtagaattc ggaatggagt cggctccatt ggattattga gtaaagcttg tattaacatc
     5041 aagctagttt tttgattgac caaaaacgtc cagcatttgc cgacgagccg attgcattcg
     5101 ctgcagcatg atcgctgaaa agccttttaa taaataatat cccaatgcat gatcactttc
     5161 gatgataggc aacaagcagg acccattgag ctttattgca gacccgcgag tcgcagcacg
     5221 cgcatcgaat tgattacgaa aagggggaaa caaccaggaa aaaccaacga taccctgtgt
     5281 caccactttt gaaacaatca gcgcttcccg gcctgggtaa tgactctcga tcgcaacact
     5341 tccactcaaa atgagataga aataatctgc tttctctccc tcggtcaata acaattcatt
     5401 ttcattgaaa tgaacgattt cactgcattc agcgagtaat ctgagatgat cctgtggaaa
     5461 ggattgaaaa aaaggcgatt ccagtaataa tgcttctata tttctaattt tcaccatttt
     5521 tcgcttcacg ctcagcttgt ttcagtgctt ttatttcttc cgttaaatcg atcccaactg
     5581 gacaccacgt aatgcaacga ccacaaccaa cacagccaga tgtcccaaac tgatcaatcc
     5641 aacttgcgag tttgtgcact aaccattgac gataacgtga ttgagttgaa gcacgcacag
     5701 cgccaccgtg ggtatatgag aaatccatcg taaaacaaga atcccaggtt tgccaccgtt
     5761 cagcatattc tccggttaaa tccgtcgtat cttcaatttt tgaacaaaag caggtaggac
     5821 aaaccatggt acagtttgca caagtcaggc atctttcttc taatttccgc cagtgcttgt
     5881 tatcgtaatt tcgataaaga aacgctttga tatcggtggt atcgatagca cgacccattt
     5941 gcccgaccgc tttctcgata catttgtcaa ccgcttctaa atcgttatta ccgacagatt
     6001 gacagggtaa ttcctgcaac acggctaatc ctcgctcact gccagattcc accacaaaat
     6061 aatgtgtacc accatcaaga atttcggtta atgcaagatc ataaccttga cttactctag
     6121 ggccggtatt tagggaagta caaaaacaag tctttccggc ttgcccgcag ttaaccgcta
     6181 caatgaaatt atctgcccgt ttctgcgtat attcagtatc ctgatactga ccgttcataa
     6241 aaacccgatc ctggatttgg atcgcatgta attcacaaga tcgaacacca ataaatgcat
     6301 agggctgaga ttgcgctacc atttcctgta ccgttttgaa actaccttca gatgttttct
     6361 gtccttgcca gactctttta cgcgcagaaa acaaaaattt tttccaagaa tgaggcccta
     6421 agttatagcc aaataaagct tgatcttctc gttgcttaat acgataagtt ccagcttctt
     6481 gctcgtctgt ccatccgatc ggcagctcat caacaacatc caggaaatcg taaatgattg
     6541 cgccttcact gatctttggg ccaaccaagc gatagccacg attctttaac gattgaaaaa
     6601 gtacatcaat atgggcccga tcaattttaa ctttttgaaa aaaattagat tttgaaattt
     6661 cccgcaagct catctgcaaa attttatgag atttatttta ctgaaccatg acagagagtt
     6721 accaatcctt gataacactg caagattctt tttgatcatg ttagatgatc attcggaaaa
     6781 agggaattta ttaaagatag ctgcaaaaac tgacaaggta tattggtaac ctgtcattaa
     6841 tggaaaatcg tagacttttt ggagcaaacc cacattttca tcaaaaattt ttctaatgaa
     6901 aatatgggtg gaattttatt gatttgctat gtacaactta aaaaactacc gcagataata
     6961 gtcaaaaatt tacttaggcc atgttatcgt taattggagt aacattatca acttctccat
     7021 tactcttttc tgctaataaa ttaggctcgg tagaagattt tgctttttgt ttcttacgaa
     7081 tattatggaa agacaatcct gttccaccag ggataagcct accaacgatc acattctctt
     7141 ttaaacctcg caactcatcc cgtttaccca taattgcggc ttcagtcaat acacgagtcg
     7201 tttcttggaa ggaagctgcc gaaatgaatg agtcagtcga aagcgaagct ttagtgatac
     7261 ctagtaatat atagttataa gctgcaggaa ttttcccttc agccaatact ttttcattct
     7321 caatcaatac atccgctctt tcaacttgtt ccccaggaat aaatgaagta tcccctgctt
     7381 cagtaatctg gacgcgacgt agcatttgtc taacgattac ttcaatatgc ttatcgttaa
     7441 tttttacacc ttgtaatcga taaacatcct gtacttcatc cgtaatatag cgcgctaata
     7501 cctctacacc ctgtaatcgt aaaatatctt gcggatcagc cggaccatcc acaatgattt
     7561 cacctttatt tacgacttga ccatcatgag ccatgacatg cttatcttta gggatcaagt
     7621 actcatgaga aacgttatca agatctgtaa taaccaggcg ttgcttacct ttggtatctt
     7681 taccaaagga cacaacacca gtcacttccg ccagcaagcc agcatctttg ggcgaacgag
     7741 cttcaaatag ttcagcaaca cgtggtaaac caccagtaat atcacgcgtt tttgaagttt
     7801 cttgcggaat tcgtgctaga acttcaccaa ctgatacttg ctgaccatta cgtaccgtaa
     7861 taatagatcc aatttggaat gtaatactta ctgatacgtc agtaccggcc actttaattt
     7921 ctttctcatt ttcatccaag aatttaacta acggtcgcaa tcctttggat tggcttgaag
     7981 tactccggcg tttcggatca ataaccacaa gtgtggacaa accggtgata tcatcaattt
     8041 gcttagctac tgttacacct tcttctacat tctcgaaacg aaccttccca ctatactcag
     8101 taatgatcgg ccgtgtatgt ggatcccatg acgcaagtac cttaccaatt ttcacggcat
     8161 caccatcatg aacgagtaat gtagctccat aaggaatttt atggcgttct ttctcacgac
     8221 cattatcatc agaaatgatc acttcaccac tccgcgaaat aataattaat tcattacgag
     8281 aattttttac ataacggata gagtttgaat attgaatcac accatttgat ttactttcga
     8341 tttgacttgc cactgcagtt ctcgaagctg ctcccccaat gtggaaagtt ctcatggtta
     8401 gctgtgtacc aggctctcca atcgattgcg ctgcaatgac accaacggct tcacccacat
     8461 caacgagaga acctcttccc aagtcacgac cataacactt agcgcataat ccataacggg
     8521 tttcacaggt aagcggtgtt cgcactttaa cttcatcaac accataaaac tcaatctgct
     8581 caaccgcatt ctcatcaagc agaaaacctg cgggatataa taccgtttga ttgtctgggt
     8641 tgattacttc ttgtactgtc acacgaccta ataacctttc tttcagtgcc tcaatgactt
     8701 caccaccttc tatcaaggct ttcataacaa caccatcttc agtgccacaa tcattctctg
     8761 taatcactaa atcttgggtc acatcgacaa gacgtcgtgt taaatagcct gaatttgctg
     8821 tttttaatgc agtgtctgct agccctttac gtgcaccatg tgtagaaatg aagtactgca
     8881 atacattcaa cccttcacga aaatttgcag tgatcggtgt ttcaatgatt gatccatctg
     8941 gttttgccat tagcccccgc atacccgcca gttgtctaat ttgagcagct gaaccccttg
     9001 cacccgaatc agccatcata tagatagagt taaatgattc ttgcatcaat ggtttaccat
     9061 gatcgtcttt cttaatttcg tttgaagatg aatcatagat attttctacg ctcaactgat
     9121 ccatcatcgc tttggcaact tgatcaccag cacgtcccca aatatcgact actttattgt
     9181 atctttcacc ttgagtaaca agtcctgaag tatattgggt tgctatctct tgtacttctg
     9241 cttcagcaga tttaataatg tcattttttt gtacaggaat ccgtaaatcg tctgcacaaa
     9301 ttgagatacc gccacgagtt gcataagtaa aacctaaatt catgagctta tcagcaaaaa
     9361 tgactgtttt acgtaatcca cataatcgga agcaggcgtt aataagcttc gatatctctt
     9421 tcttcttgag taccttattt acgagtgaaa aagagagacc ctctggtaaa acctcggata
     9481 acaatgcacg accaaccgtc gtttcatagc gctggatttt ttctgcttta gaaaccatat
     9541 cgatttcttt aattctaacc tgaattttgg cattcagctc tactgcacca ttttcgtatg
     9601 cacgaagggc ttctaccata tcctgaaaaa acatcccgtc accctgtgca tacatttttt
     9661 cacgagtcat gtaataaagg cccagaacga tatcttgaga tgggacaatt atgggctctc
     9721 cgtttgccgg cgaaagaatg ttattagtcg acatcattaa cgttcgacac tccatctgcg
     9781 cttctagtga cagtggaata tgaacagcca tttggtcgcc atcaaagtca gcattaaatg
     9841 ctgcacagac tagcggatgt aattgaatcg ctttcccttc tatcagtaca ggttcaaaag
     9901 cttgaatacc gaggcgatga agcgttggtg cgcgatttag cattacagga tgctcgcgaa
     9961 taacttcttc taagatatcc caaaccacag gagtttcatt ttccacttcg cgcttagcag
    10021 cttttatcgt gctagcaatc cccattactt ctagcttatt aaaaatgaag ggcttaaata
    10081 gctctagcgc cattttcttc ggtaacccgc attgatgaag tttgagctga ggaccaacaa
    10141 caatgactga tcgaccagaa taatcgactc gtttaccaag taaattttga cggaaacgtc
    10201 ctcctttacc cttaatcata tctgccaggg atttaagtgg gcgcttatta gctcccgtca
    10261 ttgctttacc acgacgacca ttatctagca aggaatcaac tgattcttgc aacattcttt
    10321 tttcatttct gacaatgatt tctggcgctc gaagctcaag taatcgcttc aagcgattat
    10381 ttctgttaat gactcgtcta tataaatcat tgaggtctga ggtagcaaaa cgccccccat
    10441 ccagaggaac aagtggtctt aactcaggtg gtaataccgg caagaccgtc aaaatcatcc
    10501 attcaggctt caatcccgat ttattaaatg cttcaagtac ttttaatctt tttgcaattt
    10561 tcttgatttt agtttcagag tttgtactct gcatctcaag ccggagtgaa tcaatctcca
    10621 cttgaacatt catgctattt aataaatctc gaataccttc agctccaata gcagcacgaa
    10681 aatcatcacc atattcttct gttttattta aataatcttc ttctgttaat aattgttttt
    10741 gtgtcaaggg tgtcatacca ggatcagtca caacgtatgc ttcaaaataa agcactcgct
    10801 caatatccct taaggtcata tctaatacta aacctaatcg agaaggtaag gattttaaga
    10861 accagatatg cgcgacaggg gacgctagtt caatatgccc catgcgttca cgcctaactt
    10921 ttgataatgt tacttcgacg ccgcactttt cacatataac ccctctgtgc tttaatcgtt
    10981 tatatttgcc acataaacat tcatagtctt taacaggacc aaaaattttg gcacagaaca
    11041 aaccatctct ttcaggcttg aacgtacgat aattaatcgt ttcaggtttt tttacttcac
    11101 catatgacca agaacgtatt ttctctggag aggcaagacc aatcttgatc gaatcaaact
    11161 cctctttttg agttacttgt ttaaataaat caagcaatgc tctcattttt actcctgtat
    11221 cctaatggtg ttatataaaa tgctagtctg gctttcttat tagtattgct ctaaatcgat
    11281 atccattgcc agagacctaa tctcttttac gagtacatta aatgactcag gcattcctgc
    11341 atcgatttta tggtcccctt taactatact ttcatatact tttgttctac ccccaacatc
    11401 atccgatttt acagttaaca tttcctgtaa agtatatgct gccccatagg cttctagggc
    11461 ccatacctcc atttctccaa aacgttgccc cccaaactga gctttaccac ctagtggttg
    11521 ttgcgtaacc aagctataag gccctgttga tcttgcatgc atcttatcat caactagatg
    11581 atgaagtttt aatacatgca tataaccaac cgtaaccgag cgatcaaacg cttctcctgt
    11641 tcgaccatcg taaagagtta cttgacctgt tttaggtaag tcagcaagct ctagcatggc
    11701 tttaatctca gcttcggttg ctccatcgaa aacaggtgta gcaaatggaa cacctcctac
    11761 taaattcctc gctaattcaa ttagctcgtc atcgttcagt gatgcgatat cttctttcat
    11821 accagtactg ttatatattg tttcgagaaa tggcctcaac ttagcgactc ttttttcttg
    11881 ttgtaataat gctccaattt tctcaccaag acctttagat gcccacccca gatgtacttc
    11941 taatatttga ccgacattca ttcgcgatgg cacacccagt ggattcaata cgacatcgac
    12001 tggcgtaccg tctgccatgt aaggcatatc ttctattgga acgattttcg aaataacccc
    12061 tttatttcca tgccttccag ccatcttatc gccgggttgt agccgcctct taacagccac
    12121 ataaacctta accatttttt gaacacctgg cggtaattca tcaccttgag tcaatttctt
    12181 tcgtttttct tcaaacgcac tatcaaacaa tttttgtttt tgttctaacc cttctttcat
    12241 ctgttctaat tgccggctta tatcttcatc agccaatcga atatcaaacc agtgatgcaa
    12301 atcaatacta tcgagatact ctttggtaat tgctgtacct tttgctaatt ttttgggtcc
    12361 acctgtaact acttttccaa taagtaatct ttcaaggcgc ttaaatacat ctgtttcaac
    12421 gatgcgcatt tgatcagcca gatctttttt gtaccgatct aattcatcat taattatttt
    12481 tttggaacgc ttatcacgct gtaccccttc tctggtgaaa acttgtacat cgataaccgt
    12541 tccagagatt ccagatggta ctcgtaagga cgtatcctta acatctgagg ctttttctcc
    12601 aaaaatagcg cgcagtaatt tttcttctgg tgttaactgc gtttcgcctc taggtgtaac
    12661 tttaccaacc aaaacgtcac ccacctctac ttcagcgcca atatatacaa tccctgactc
    12721 atcaagccgt gatagctgac gttcggatag attaggtata tcagcagtaa tttcttcagt
    12781 tccaagcttt gtatcgcgac tcactaccga aagttcttcg atatgaattg atgtaaagcg
    12841 atcctcagct acaactcgtt cagaaataag aatcgagtct tcaaagttgt aaccattcca
    12901 tggcataaat gcaacgagca tgttttgccc taatgcaagc tcccccatat cggttgatgc
    12961 gccatctgca atgacattat tacgttcgat tacatcacca atcttaacaa gcggccgttg
    13021 attaatattt gtgctttgat tggatcgggt atatttgatc aagttataga tatccacccc
    13081 gacatcgccc gcgagggttt cttcatcatg aacacgaatg actattctat ttgcgtcaac
    13141 aaaatcaaca accccacctc ttattgccct tacagcagtt ccagaatgct cagcaaccac
    13201 gcgctctatc ccagttccta caagtggttt ctctgcacgc aaacaaggta cagcttgacg
    13261 ctgcatattt gaacccatta atgcacgatt tgcatcgtca tgctccaaaa aaggtattaa
    13321 tgaagcagct acggatacaa tctgtgcagg cgcaacgtcg acataatcaa tttgatcttt
    13381 tggagagagt gtgaattcgt tcctatgacg acacgaaaca atttcatcaa taaatcgccc
    13441 atcagaatct aactttgcat tcgcttgtgc aataacgtac tgactctctt caattgcaga
    13501 aagaaataca atttgatctg ttacagttcc ttcgacaact tttctgtaag gtgtttcaat
    13561 aaaaccatat tcattagttt gagcataaag cgcaagagaa ttaatcaatc ctatatttgg
    13621 tccttctgga gtttcaattg gacaaacacg cccataatgg gttgtatgca cgtctctaac
    13681 ttcaaatcca gcacgctcac gtgttaagcc acccggccct agcgccgata tacgacgctt
    13741 atgtgttact tcagaaagcg gattcgtttg atccatgaat tgtgataatt gacttgaacc
    13801 aaaaaattca cgggctgcag ctgataccgg tttagcatta attaaatcat gaggcattaa
    13861 attctctgat tccgcctggc ttaaacgctc tttaacagct ctttcaactc gcgccaggcc
    13921 tgctctaaat tggttttctg ctaattcccc aactgaccgc actctacgat ttcccaagtg
    13981 atcgatatca tcaatttcac ctcttccatt tcgaagctct actaagattt tgattacatc
    14041 aataatatct tggtgagtta acgtaggtag tccttctaat tctgctctcc ccacacggcg
    14101 attgaatttc attcgaccca ctactgatag atcgtaacgt tctgctgaga aaaataaacc
    14161 attaaacaga gcaataaccg cttcttcagt tggtggctca ccagggcgca tcatgcgata
    14221 gatagctact tgagcagaaa actgatcagc gacttcatct gttcgtaaag ttagggaaat
    14281 ataaggtcct tgattgagat catttatata aagcgttgta actgctttaa tcccagcaac
    14341 tcttattttt tctagtattg tttcagtaat ctcatcgttt gctagcgcaa tgatttcacc
    14401 agttttttga tctataatat ttttagctag aattttcccg agcaaaaaat cttctggaac
    14461 ttcaattttt tctatttggg actgctgtaa atccctaacg tgtttagccg tgattcgctt
    14521 atctttttgg attattatat cgccactgct agatacgatg tcaaagcttg ccacctcacc
    14581 gcgaagtctc tcgggaacaa ggtcaaacaa aattttgttt ttagaaaaat gaaaatgatc
    14641 aaaaacaaaa aaatctgcga gaatttgctc aggagtacag cccatggctt ttaatagcgt
    14701 agtcacaggc attttacgtc tacgatctat acgaaaataa acataatctt tagaatcgaa
    14761 ctcaaaatca agccaagagc cgcgataggg aataattctt gcagaaaaaa gtaacttacc
    14821 tgatgagtgc gttttccctc ggtcatgctc aaaaaataca ccaggagaac gatgaagctg
    14881 ggacacgata actcgctctg taccatttat gacaaatgac cctgtttcgg tcataagagg
    14941 aatctctccc atatacactt cttgctcttt aacttctttt acagttggtt ttgaagattc
    15001 tttatcaaga atagtcaacc ggacttttgc tcttaaagga gaagcataag ttaaccccct
    15061 ttgctgacat tccttcacat caaagggtgg atgtcctaaa ttgtagctaa tgaaatctaa
    15121 acgagcattt ttagaatgac tttcaatagg aaaaatcgaa ttaaatgctg cttgtagacc
    15181 gatatttttt cgagaatttg ctagcttatt agcttgtaaa aacgatgtat aggattccag
    15241 ctgagttgca agaagaaatg gtatggataa tacacttgct cgctttgcaa aactcttacg
    15301 tatgcgtttt ttctcggtga acgaatagtc catcaacttt ctccaggaac agaagattca
    15361 atgtaaatac cgcttattga atctaactaa aaagtggtta cctcaactat caagactgaa
    15421 tttacattag tcaaatggag caaaggctga tagccagcga ttgctatcag ccaaaactat
    15481 taatttagta ttaactgtta tcgctattat ttaatatcac atgccgcacc agcttctgta
    15541 aattgtttgc gaatcgcctc tgcatcagct ttagcaattc cttctttaac aacttttggt
    15601 gctccgtcaa ctaaatcttt agcttccttc aatccaagac ccgtcacagc gcgaactact
    15661 ttaattacat taactttatt atctccagct gaagtcagaa tgacagaaaa ttctgtttgt
    15721 tcttcagcag ctgatgcagc tgctgcagcc ggtgcagcaa cagctactgc agc
//
LOCUS       DAOQKQ010000047        15728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_1721_length_15728_cov_5.720666, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000047 DAOQKQ010000000
VERSION     DAOQKQ010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 15728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15728
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1721_length_15728_cov_5.720666"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="PKZ49_04575"
     CDS             complement(<1..636)
                     /locus_tag="PKZ49_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HND35871.1"
                     /translation="MSQTITIIQPDDWHLHLRDGEQMQCVLPDSAIRFARAVIMPNLK
                     PPIITTDQAIQYRSRIIESLPNALRSHFNPLMTLYLTDNTSPQEVAKAKKSGMVHAVK
                     LYPAGATTNSDAGVTDLSKCFATLASMEQLGLPLLVHGEVTDPAVDIFDRESVFIDRV
                     LTPLIRRFPVLRIVLEHITTREAVEFIRSTPNHIAATITAHHLLFNRNAIFT"
     gene            complement(668..1519)
                     /gene="rsmI"
                     /locus_tag="PKZ49_04580"
     CDS             complement(668..1519)
                     /gene="rsmI"
                     /locus_tag="PKZ49_04580"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HND35872.1"
                     /translation="MQQNNGTLYVVATPIGNLRDISLRALDIFALVDRIAAEHADLTR
                     HLLSHHGIKTPLMTLHQHNEHLAVEKIANLLANNQDVALVTDAGTPCISDPGAMLVHA
                     MRALGHKVVPIPGANAAICALSASGIRSTSFFFYGFLPVKSGERQRCLASLTQLLSCT
                     LIFYEAPHRIVDCINDMIAVFGVHRQITLARELTKVFETFHSDNLENTLQWLLADENQ
                     QKGEFVLLLPPFLEENEPGLSRDAQHTVQVLMKELPLKQVVQLAVSITGEKKKSLYAY
                     ALSIKND"
     gene            1621..1959
                     /locus_tag="PKZ49_04585"
     CDS             1621..1959
                     /locus_tag="PKZ49_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HND35873.1"
                     /translation="MNGSEAEDLAAHYLQQQHLVLVERNYRCRYGEIDLIMRDQTMLV
                     FIEVKMRTHTSFGGAIHSITESKQHKLILTANHYLANLKKIPPCRFDVVLITGQLAHE
                     INWIKNAFDE"
     gene            complement(1965..2711)
                     /locus_tag="PKZ49_04590"
     CDS             complement(1965..2711)
                     /locus_tag="PKZ49_04590"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HND35874.1"
                     /translation="MTSNNKVILITGGAKRVGAAICRQLHAQGANLAIHYRSSQEEAE
                     ALRDSLEAMRRQSVLLIEADILNVDQLPNLIEQIILRFGQLDAVVNNASSFFSTPIGR
                     FSDAAWNDLIGSNLKAPLFLSQAAAPYLIKQKGCIINIVDIHVDYPLKNHVIYNCAKG
                     GLAALTRSLALELAPDVRVNGVSPGPILWPVTGDWADTSLQHEIINRTLLKRCGEPDD
                     IAKAVNYLINDAPFVTGQIIAVDGGRSINL"
     gene            2791..3975
                     /locus_tag="PKZ49_04595"
     CDS             2791..3975
                     /locus_tag="PKZ49_04595"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HND35875.1"
                     /translation="MPSSNLPQPTQEGLQHSQHLQSYIVNKIHESGGWIPFAQFMELA
                     LYTPGLGYYSASNVKFGFNGDFVTAPEISALFGRTLAKQAMQLFHQIGKTSILEFGAG
                     SGKLALDLLMELEQFNQLPEQYLILEISAELQQRQRMLFETHAPQFLDRITWLAQLPD
                     QFSGLIIANEILDAMPTHLILWQKEALFERGVSANQQTFSWVDKPLNHDELLTIANQL
                     SVFIERDRSDFVSYLSEVSLANRYFIRSLSEILQAGLILLIDYGFGQREYYHPQRSMG
                     TVMCHYRHFAHDDPFYLPGLQDITSHVDFSAIAQAASESNLQLLGYTNQAHFLINCGI
                     TELLSSVAVTDTLKYLPLSNQLQRLVSPAEMGELFKVIALGKRLSEIPVGFTRGSLLH
                     LL"
     gene            complement(3970..4707)
                     /locus_tag="PKZ49_04600"
     CDS             complement(3970..4707)
                     /locus_tag="PKZ49_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylase"
                     /protein_id="HND35876.1"
                     /translation="MSLIGIVTAMRAEAKCVTSRRLPFNEMVRLREGTAIWICGIGEA
                     AAHKAAIGLRDQGVSALVSFGVAGALSASLRPGDLVLPEHIQIEESDRVSVALAWRAR
                     IKQLLPKHVNVAGGTLATSRQMLSSEAAKRAFSETHGACAVDMESGAIAKVAAETGIP
                     FVAVRAITDPLEFSPPPILMDAVYPDGSVNIPQLLNLLIRRSINISTLLQLAPSMRAA
                     CSTLLSVAQHADNELGGQFSSVAIANS"
     gene            complement(4713..6704)
                     /gene="shc"
                     /locus_tag="PKZ49_04605"
     CDS             complement(4713..6704)
                     /gene="shc"
                     /locus_tag="PKZ49_04605"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HND35877.1"
                     /translation="MSRISQQFNDLINLKKIALKDTWFKSKATSPTSSSDVAQSELVQ
                     CITKARNALMALQNKDGHWCFPLEADCTIPAEYILMMHFMDEIDVELEQKLAIFIREQ
                     QSEVHGGWALYYGGEFDLSCSVKSYFALKIVGDSPDAPHMQRVRAAILERGGAASANV
                     FTRLLLAMYQQIPWRGVPFVPAEIILLPRWFPFHLRKVAYWSRTVMIPLSILCTLKAK
                     AANPRKIGIRELFTIPPEEEQNYFPVRTSLNRILLYVERLGSKLEPMIPTFIRKIALK
                     RAEKWVIERLNGECGLGAIFPAMVNAYEALTLLGYDAEHPYRKQCKAALQGLLVDEGR
                     RAWCQPCTSPIWDTVLASLSLQEDSHADQEPVKQALDWTIQRQILDEPGDWREAKPNL
                     PGGGWAFQYENPHYPDLDDTAAAAWALYQADPEVYRFSLRRAADWLAGMQSSNGGFAA
                     FDIDNTYYYLNEIPFADHGALLDPPSSDVTARCTGFLSLYGDPQHQQVVERGLAYLFN
                     EQESNGSWFGRWGSNYIYGTWSVLEAMQLAKVDRQHEAIRKAVQWLKSKQRPDGGWGE
                     TNDTYLDPRQAGEFNVSTSFQTAWALLGLMAAGEVNSTAVQKGISYLMKTQADDHLWY
                     EPWFTAPGFPRVFYLKYHGYSKYFPIWALIRYRTLTTER"
     gene            complement(6841..7722)
                     /locus_tag="PKZ49_04610"
     CDS             complement(6841..7722)
                     /locus_tag="PKZ49_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HND35878.1"
                     /translation="MKARCNTIILLGIFITALMACTSAPKKESLADTQQVTAHQGSNA
                     EIHSEHSEEMPVDPYESANRKTYSFTDSIDRFILEPVADVYIDYVPYAIQRPISNFYR
                     NLSYPNVALNAFLQGKFRQGFEDSFRFVINSTIGIFGLADMAGHMGFKENNEDFGQTL
                     AVWGVDPGSYLFVPIYGPSNRRDILDLPIGFFTDALFYASYAISGPALVPILFLRVVD
                     KRARLAGPMRIRDESALDPYLFVREAYTQQREYLIHDGNPPIEQYDDIEEEGDEKDKV
                     KDAVKKEKIEDAKSNKD"
     gene            complement(7823..8842)
                     /locus_tag="PKZ49_04615"
     CDS             complement(7823..8842)
                     /locus_tag="PKZ49_04615"
                     /inference="COORDINATES: protein motif:HMM:TIGR03466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HND35879.1"
                     /translation="MNNKTCSLVTGGGGFIGMHLVKQLLDQGEAVKVLEISDTTFPKE
                     VEVIQGSITDVDTVRFALKGVDRLFHLAANPYLWAHNKKIFDQVNYEGTRVVLSEAKR
                     ANIKKVVHTSTESILSTKTSKNQPIHSSVVPILEDMPGPYCRSKFLAEQEAFKASQEG
                     LPVVIVSPTLPIGPGDRRLTPPTQMILNFLNHKIPAYLDCKLNVVDVRDVARGHILAA
                     ERGRPGERYILGNQNIQLSKLLQLLEEISGLVMPKTKIPHWLALIFSVFSELTADYIT
                     RKPPQAPLTGVRLAGRSLFFESDKAVRELGFKQSSIRQALIDQVEWLLQEGYVTRSMP
                     QRQRA"
     gene            complement(9041..9649)
                     /locus_tag="PKZ49_04620"
     CDS             complement(9041..9649)
                     /locus_tag="PKZ49_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HND35880.1"
                     /translation="MLSIIQAAGWPIWPIIIASIVAAAIIGERIWSLRSSKVSPRDLL
                     SKVLHDYRRSGINASTLNRLQENSPLGCVLAAGLKNIESSREVMKESIEEAGRVVAHE
                     LDRYLTTLGTIASLSPLMGLFGTLIGMIEIFGSNSPTGSNPAQLAHGISIALYNAAFG
                     ILVAIPSMIFYRHFRAKVDALVLEMELQALKLVEVVHGDRKI"
     gene            complement(9836..10147)
                     /locus_tag="PKZ49_04625"
     CDS             complement(9836..10147)
                     /locus_tag="PKZ49_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35881.1"
                     /translation="MTTRKLTLTLALVGVLLLSGCVTTPYFSGVNGRYGNVSFSYGYR
                     PPVAISPGYYTSHRQVYVTPRYYNRGHGHFIGGHRHFGGGHKHFIGGHRGGFRGGHFR
                     W"
     gene            complement(10318..10974)
                     /locus_tag="PKZ49_04630"
     CDS             complement(10318..10974)
                     /locus_tag="PKZ49_04630"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HND35882.1"
                     /translation="MKKQPDFIPALHYHWLTRWYDPVMRTIFPETRITDMLIRQANIQ
                     SGERVLDVGCGTGTHTIRVKRAQPEAIVHGVDIDPEVLKLAQRKIEQSHIELLLKQAS
                     ANALPYPDNHFDHIISSLLLHHLKPVFKQKMLEEAFRVLKPGGRLHLIDFGEPHDMSM
                     WVISLIVRWFEEIHSHIHGCLPKLIQEAGLNLMPMSLHFRTLGGTITLWHAYKPTEVY
                     "
     gene            complement(10990..12087)
                     /locus_tag="PKZ49_04635"
     CDS             complement(10990..12087)
                     /locus_tag="PKZ49_04635"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HND35883.1"
                     /translation="MKILLIIVLLSMLYSLSWPVPIEPESWKAPSAPGYTDQHAVNQR
                     LGQLNQIHLQGYEGPEHVAISPTGQLYTAVVDGSLLRLNPDGSDLEVFVNTGGRILGF
                     DFDVSGNIIAADALKGVLKITPDRRIEVLVDEVEGKPIRYPNSVVVASNGTIYFTDSS
                     SRFVPADWDGTFEATMLDIVEQSATGRVIAYDSLSGRTRVIAQGLSFANGIALSHDEQ
                     TLFVVETGRYRLWKIAILANAITIGDESPDASVLLDNLPGYPDNLMRGLEGRIWLGLA
                     KPRNTAIDFMSDKPLLRKMVLRFPRFLWPMPESYGHVIAFTETGQIVVDLQDPTGDYP
                     ETTGVTETNERLYIQSLHAKSLGWLDKSILK"
     gene            12214..13632
                     /locus_tag="PKZ49_04640"
     CDS             12214..13632
                     /locus_tag="PKZ49_04640"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HND35884.1"
                     /translation="MLRKILIANRGEIAVRIIRACAEMGIRSVAIYSEADRFALHIKK
                     ADESHYIGSEPMAGYLNIHALVDLALATGCDAIHPGYGFLSENAEFAKVCKERGLVFI
                     GPTPEVIHRMGDKTEARNAMKAAGIPITPGSEGNVNSVKEALNVAEQIGYPVMLKATS
                     GGGGRGIRRCDTAQELERNYTRVISEATKAFGRAEVFLEKCIVNPKHIEVQILADHHG
                     NVIHLYERDCSIQRRNQKLIEIAPSPQLDEAQRQYIGGLAVIAAKSVGYTNAGTVEFL
                     LDDSGRFYFMEMNTRVQVEHTITETITGVDIVEEQIRVAAGLKLRFKQDEIVRRGYAI
                     QFRINAEDPKNNFLPSFGRISRYYAPGGPGVRTDTAIYTGYEIPPFYDSMVAKVIVSA
                     LTWEDVIKRGERTLRDMGLFGIKTTIPYYLKILNHPQFRAARFNTSFVEQNPQLINYS
                     NKPRPEVLASVIAAAVASHTGL"
     gene            13647..15491
                     /gene="oadA"
                     /locus_tag="PKZ49_04645"
     CDS             13647..15491
                     /gene="oadA"
                     /locus_tag="PKZ49_04645"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HND35885.1"
                     /translation="MRQEKSKQTVHITDVILRDAHQSLIATRMRTEDMLPACTLLDQI
                     GYWSLECWGGATFDTCLRFLKEDPWERLSQLKAALPNTPLQMLLRGQNLLGYRHYSDD
                     VVHAFIQRAATNGITIFRIFDALNDVRNLKTAIEAVKSAGKHAQGTICYTTSPVHDVK
                     SFVSLASDLAKLGCDSIAIKDMAGLLTPFATQELVSALKQTVDLPIHLHSHATSGLAE
                     MCQLKAIEAGCRHIDTALSSWSGGTSHPPTESLVMALQGTPYDTKLDLQKLQAVNDYF
                     AQIRKKYHRFESEFTGVDTRVHVFQVPGGMISNLANQLQERNALDRINEVYAEIPRVR
                     KDLGYPPLVTPTSQIVGTQAVLNILTGKRYSTITNEVKRYLQGGYGKAPAAINASLQK
                     SAIGKEDLIDCRPADLLKPEFEQLRKEIGQLALNDEDVLSYAMFPEIGKQFLEQRSSG
                     QLVPEPLEVASTTVNGVQKAPTEFNIALHGESYHIKVTGTSPKTESTRHFYFMVDGIP
                     EEILVETLDEIVLDGGTQGPVKSAIASKRRSPTEKGDVVVSMPCNILDVLVKIDQKIT
                     AGQPVLVTEAMKMETEITAPISGIVKAVHVVKGEAVNPNEVLIEIVAG"
     gene            complement(15520..>15728)
                     /locus_tag="PKZ49_04650"
     CDS             complement(15520..>15728)
                     /locus_tag="PKZ49_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35886.1"
                     /translation="FLVYKTSATVKLILQDRLSTCVRLSATGALPLGRATFPASASPG
                     IFEVCSLCFCPLRGAGAEPLRVIF"
BASE COUNT         4586 a         3650 c         3182 g         4310 t
ORIGIN      
        1 ggtaaaaatt gcattacgat taaataacaa atgatgtgcc gtgatagttg ctgcaatgtg
       61 gttaggtgtg ctgcggataa attcaaccgc ttcgcgtgtt gtaatatgct ctaaaacaat
      121 gcgtagtact ggaaaacgtc gaatcaaagg ggttaataca cgatcaataa acaccgattc
      181 ccggtcaaaa atatccacag caggatcggt tacttcacca tgcactaata atggcagccc
      241 aagttgctcc atagacgcta gcgtagcaaa gcattttgaa agatcggtaa cacctgcatc
      301 tgaatttgtt gtagcacctg ccggataaag cttcacggca tgcaccatac cactcttttt
      361 agcctttgct acttcttggg gggaagtatt atcagtaaga taaagtgtca tgagtggatt
      421 aaaatgacta cgcaatgcat ttggtaatga ttcaatgatc cgcgagcgat attgaattgc
      481 ttgatcagtg gttataatcg gtggcttaag gtttggcata ataaccgctc gagcaaaccg
      541 gatcgctgaa tcaggcaata cgcactgcat ttgctcgcca tcgcgcaaat ggagatgcca
      601 gtcatccggt tggataatcg tgatcgtttg agacataaat tttttagatt aatctaatca
      661 agtcatgtta atcattctta atcgacaaag catacgcgta gaggcttttc tttttctcac
      721 ccgtaatcga taccgcaagc tgcactactt gctttaacgg taactccttc attaatactt
      781 gtaccgtatg ctgtgcatct ctggaaaggc caggctcatt ttcctcaaga aaagggggta
      841 atagcaatac aaattcccct ttttgttgat tttcatctgc caatagccat tgcaaggtat
      901 tctctaaatt atcgctatga aacgtttcaa atacctttgt taactcccta gcaagggtaa
      961 tttgccgatg cacgccgaag acagcgatca tatcgttgat acaatcaacg atacgatgtg
     1021 gtgcttcata aaaaatcagc gtacaagaca gtaattgagt taatgaagcc aggcagcgtt
     1081 ggcgttcacc tgattttacg ggtaggaaac catagaaaaa gaaagaggtt gatcgaatac
     1141 ccgatgcgga taaggcacaa attgctgcat ttgcacctgg aatcggaaca accttatgtc
     1201 ctaaagcacg cattgcatga accaacatcg cgcctggatc agaaatacag ggtgtacctg
     1261 catccgttac caatgcaaca tcttgattat ttgcaagcaa attagctatt ttttcaaccg
     1321 ccaaatgttc attatgttga tgcagtgtca tcaaaggtgt tttgatacca tgatgcgaca
     1381 ataaatgtcg ggtcaaatcc gcatgctcag ctgcaatccg gtcaactaga gcaaatatat
     1441 ccagtgcacg taagctaata tcacgcaaat taccaatcgg tgttgccact acatataatg
     1501 taccgttatt ctgctgcatt gactctgttt actgacgctg tttaatatcg gtaaattgtc
     1561 gcacacactc gtttgcttta gaaagcaaaa gtacgctaat gggatcattt ctgacttaat
     1621 atgaatggta gtgaagctga agatttagct gcacattatt tgcaacaaca acatttagtc
     1681 cttgttgaaa gaaactatcg ttgtcgctat ggtgaaatcg atttgatcat gcgcgatcaa
     1741 acaatgcttg tttttattga agtcaaaatg cgcactcaca cttcgtttgg aggtgctatt
     1801 catagtatta ctgaatcgaa acaacataaa ctcatactga ctgcaaatca ttatttagct
     1861 aacctaaaaa aaattccacc ttgccggttt gatgtcgtgc taattaccgg gcagctagcg
     1921 catgaaatca actggattaa aaacgctttt gatgaatgat tgaattaaag attaatacta
     1981 cgcccgccat ctacagcaat aatttggcca gtcacaaatg gagcatcgtt aatcaagtaa
     2041 ttaactgctt ttgcgatatc atccggttca ccacagcgtt tcaacaatgt tcgattgatt
     2101 atttcatgct gtaatgatgt atccgcccaa tcaccggtta ccggccatag aatcggaccg
     2161 ggtgaaaccc cattgacgcg aacatcaggc gctaattcta aagctaaaga gcgagtcaac
     2221 gctgctaatc cgcctttcgc gcaattatag atcacatggt ttttcaaagg ataatctaca
     2281 tgaatatcca caatattgat aatacaaccc ttctgtttga ttaaataagg ggcggctgct
     2341 tgagaaagaa aaagcggtgc tttaaggtta ctgccgatca aatcattcca tgctgcatca
     2401 gaaaatcttc cgatcggtgt tgaaaaaaaa ctcgatgcat tattcactac agcatcgagc
     2461 tgtccgaagc ggagtattat ttgttcaatt aaatttggca actgatctac gttgagtata
     2521 tcagcttcga ttagtaaaac tgactgtcga cgcatcgctt ccagtgagtc acggagtgcc
     2581 tcagcctcct cttgagaaga acggtaatga atggcaagat tggccccctg cgcatgcagt
     2641 tgtcgacata tggctgcgcc cacacgtttt gctccgccag taatcaaaat tactttatta
     2701 tttgatgtca tccatttccc cctttattca tccttcgccg ctcgctatca ttctctaact
     2761 ctttcacatc acttacacac gattactttc atgccatcat ccaatctacc ccaacccact
     2821 caggaaggat tgcagcatag tcaacattta caaagttata ttgtaaataa gattcacgaa
     2881 tcaggggggt ggataccttt tgcccaattt atggaattag ctttatatac ccctggtttg
     2941 ggttactaca gtgcaagcaa cgttaaattt ggatttaacg gtgattttgt cactgcgcct
     3001 gaaatatcgg ctctttttgg aagaacgctg gcaaaacaag cgatgcagct atttcaccaa
     3061 atcggtaaaa ccagcattct tgaatttgga gctggatcag gtaaattagc gctcgatttg
     3121 ttaatggaac ttgaacaatt caatcaatta cctgaacaat atctcattct cgaaataagc
     3181 gctgaattac agcagcgtca gcgaatgcta tttgagactc atgcaccgca attccttgat
     3241 cgcattactt ggttagctca attacctgat caattttcag gtcttatcat tgcgaatgaa
     3301 atactcgacg ccatgcctac ccatttaatt ctttggcaaa aggaagcgct ttttgagcgc
     3361 ggtgtcagtg ccaatcaaca aacattttct tgggtagata aacctttgaa ccatgatgaa
     3421 ctactaacaa ttgcaaacca attgtcggta ttcattgaaa gggatagatc tgattttgta
     3481 agctacctaa gtgaggtttc acttgccaat cgatatttta ttcggagctt aagtgaaata
     3541 ttgcaagccg gattgatttt gctcatcgat tatggtttcg gccaacgaga atactatcat
     3601 ccccagcggt cgatgggtac tgtcatgtgt cattaccgcc attttgctca cgacgatcca
     3661 ttctatttac cagggttaca agatatcacg agccatgttg atttttcggc cattgctcaa
     3721 gctgcatctg agagtaactt acaattgtta ggctacacca atcaagctca ttttctaatt
     3781 aactgtggta ttactgaatt attatcgagt gtagcagtca ctgatacact aaagtattta
     3841 ccgttatcaa atcaattgca gcgactagta agtccagctg agatggggga gctatttaag
     3901 gtaattgccc tgggcaagcg attgagtgaa atacccgtcg gttttacccg aggtagctta
     3961 ttgcatttgt tatgagtttg cgattgcaac tgaagaaaat tgcccaccga gctcattatc
     4021 agcatgttgc gctactgaaa gcagtgtcga gcaagcagct cgcatacttg gcgccaattg
     4081 cagcaatgtg ctgatattga tcgagcgacg tattaacaga ttcaataatt gaggaatatt
     4141 tacgcttcca tcgggatata ctgcgtccat caaaattggc gggggtgaga attctaatgg
     4201 gtcggtaatt gcacgtactg caacgaatgg tatgccggtt tctgcggcta ctttagcaat
     4261 tgcgccactt tccatatcga cagcacaggc tccgtgcgtt tcagagaatg ctcgctttgc
     4321 tgcctcagat gatagcattt gtcgactggt agccaaagtt cctccggcaa cattgacatg
     4381 tttcggtaat agttgcttta tgcgggcccg ccatgctaag gccactgaaa ctcgatcgga
     4441 ttcttcaatt tgaatgtgct caggtaatac gagatcaccg ggccgtaatg atgcacttaa
     4501 tgcacccgca acaccaaaac tcaccaacgc tgatacacct tgatctcgaa gtccgattgc
     4561 agctttatga gcagcggctt ccccaattcc acaaatccaa atcgctgtac cttcccttaa
     4621 gcgaaccatt tcattgaaag gtagacgtcg cgacgtcaca catttagctt cagcacgcat
     4681 cgcagtaaca atgccaatta gactcacaac tgttacctct cagtagtcaa agtgcgatag
     4741 cgtattagtg cccatatagg aaaatatttc gagtaaccgt gatatttcaa ataaaaaacc
     4801 cgcggaaaac caggcgctgt aaaccaaggt tcataccata agtgatcatc agcttgggtt
     4861 ttcattaaat agctaattcc tttttgaact gctgtactat taacctcgcc tgctgccatc
     4921 aaacctaata aagcccaggc tgtttggaag gaagtgctta cattaaattc ccctgcttgc
     4981 cgtggatcaa ggtaagtatc attagtttct ccccagccac catcaggtcg ttgttttgat
     5041 ttcagccatt gtaccgcttt ccgaattgct tcatgttggc ggtctacttt ggcaagttgc
     5101 atcgcctcta ataccgacca cgtaccgtaa atatagttac ttccccaccg cccaaaccag
     5161 gatccattgg actcttgttc attaaacaaa taagctaaac cacgctcaac tacctgctga
     5221 tgttgaggat cgccatataa agataagaaa cctgtgcagc gcgctgtaac atcgcttgag
     5281 ggtggatcaa gtaacgcacc atgatcagca aatggaattt cattcagata ataataggtg
     5341 ttatcaatat cgaaagctgc aaacccgcca ttacttgatt gcatgcctgc aagccaatca
     5401 gctgcacgtc gcaagctaaa tcgatatact tccggatcag cctgatacaa tgcccatgct
     5461 gcggcagcag tatcatctaa atctgggtaa tgggggtttt catattgaaa ggcccatcca
     5521 ccccccggta aattgggctt tgcttcacgc caatcacctg gttcatcgag aatttgccgc
     5581 tgtatcgtcc agtccagcgc ttgcttaaca ggttcctgat ctgcatggga gtcctcctgc
     5641 aatgataagg acgctaacac agtatcccaa attggcgaag tgcaaggctg acaccatgcc
     5701 cttctgcctt catcaaccaa taatccctgt agcgctgctt tacattgttt acgataagga
     5761 tgctccgcat cgtaaccaag taatgtaagc gcttcatatg cattaaccat ggctggaaaa
     5821 atggcaccta aaccacactc accattcaaa cgctcaatga cccatttttc agcgcgtttg
     5881 agtgctattt ttctgatgaa tgtcggtatc attggttcga gcttggagcc caaccgttcc
     5941 acatacaaca aaattcgatt gagtgatgtg cgaactggga aataattttg ttcttcctcg
     6001 ggcggaatag taaataattc tctgataccg atctttcgag ggttagctgc ttttgctttc
     6061 agcgtacaaa gaatagacaa cggtatcatg accgttcgcg accaatacgc tactttcctt
     6121 aaatgaaatg gaaaccagcg tggtaataaa ataatttctg caggcacgaa cggcacaccc
     6181 cgccaaggta tctgttgata catggcaagc agtaagcgcg taaacacatt agcacttgcc
     6241 gcgccaccgc gttcaagaat cgctgcacgc acacgttgca tatgcggcgc atcaggcgag
     6301 tcgccgacga tttttaatgc aaaatacgat ttaacgctac aacttaaatc aaattcccca
     6361 ccataataga gtgcccaccc cccatgtact tcgctttgct gctctcgaat gaaaatagcg
     6421 agtttttgct ctaactcgac atcaatttca tccatgaaat gcatcatgag aatatattct
     6481 gcaggaatcg tacaatccgc ttccagcgga aagcaccaat gtccatcttt attttgtaaa
     6541 gccattaagg cattacgcgc ttttgtgatg cattgcacta attcagattg cgcaacatcg
     6601 ctagatgagg ttggtgaagt tgctttcgac ttaaaccatg tatctttcaa agcaattttc
     6661 tttaaattaa ttaagtcatt gaattgctgt gatattctag acataaatta aagccttacc
     6721 gttaactaat tgtaatcaaa gcattttaca agaaacgtta attcagtcac tgtttattaa
     6781 tgaaaaaaac aacggactgt cgtatatacg atacagctag gcggagaaag gtgataatta
     6841 ttaatcttta ttacttttag catcttctat tttttctttc ttaactgcat ccttcacttt
     6901 atctttttca tcaccttcct cttcaatatc atcgtattgt tcaataggag gattgccatc
     6961 atgaatcaga tattcgcgtt gttgcgtgta ggcttctcga acaaacaagt aagggtctaa
     7021 tgctgattca tctcgtatcc tcatcggccc agccaatctt gcgcgtttat cgaccactcg
     7081 taaaaataga atcggtacta acgcaggccc actaattgca tagcttgcat aaaatagcgc
     7141 atcggtaaag aatcctatcg gtaaatccaa aatatcccgg cgattacttg gaccatagat
     7201 tggaacaaaa aggtaagacc ccggatcaac tccccaaact gcaagtgtct gcccaaaatc
     7261 ttcattattt tctttaaatc ccatatggcc agccatatca gctaggccaa agataccaat
     7321 cgtagagtta atcacaaaac gaaagctatc ttcaaatcct tgacgaaatt tcccttgcaa
     7381 aaaagcattc aaagcaacat taggataaga taaattgcga taaaaattgc ttatgggccg
     7441 ttgaattgca tagggaacat aatcaatata cacatccgca actggctcta gaataaatcg
     7501 atcaatgcta tcggtaaacg aataggtttt acggttagcc gattcatagg gatcaacggg
     7561 catttcttct gagtgctcgg aatgaatttc ggcatttgat ccctgatgag cagtaacttg
     7621 ctgggtatct gccaatgatt ccttcttagg tgccgacgta caagccatca aagcagtaat
     7681 gaatataccc aataaaataa ttgtattaca gcgtgctttc atgctacaga attcaaaagt
     7741 gatcgataag aaactaaatt taatatatta acttactgat attttttcaa cttacctaaa
     7801 acaactgcgt atacatccat gattatgcgc gttgcctttg cggcattgat cgtgttacgt
     7861 agccttcttg taataaccat tcaacctgat cgatcaacgc ctgccgaata gagctttgtt
     7921 taaaacctaa ctcacgcact gctttatcgc tttcaaaaaa taatgaacga cccgctaatc
     7981 ttacaccggt taacggtgct tgaggcggct tacgagtaat ataatccgct gtcagttctg
     8041 agaaaacact gaaaatgagc gctagccagt gcggaatctt agttttaggc attaccaaac
     8101 cgctaatttc ttccagcaac tgcaacaatt tgcttaattg gatattttga ttaccgagga
     8161 tatagcgctc accggggcgg cctcgttccg ctgctaaaat atggcctcgt gcaacatctc
     8221 ttacatcaac cacattcaat ttacagtcta agtaagctgg aattttatga tttaggaaat
     8281 ttaaaatcat ctgtgtcggg ggcgttaatc gacgatcacc gggtcctatc ggtagcgttg
     8341 gagaaacaat gaccaccggt aaaccttcct gtgaagcttt aaaagcttcc tgctctgcta
     8401 agaattttga gcgacaatag ggtccgggca tatcttcaag aatcggtacc actgaagaat
     8461 gaatcggttg atttttcgaa gtcttcgtgg aaagaatcga ttcagtcgag gtgtgaacca
     8521 ctttttttat attcgcgcgt ttagcttcag ataacacaac gcgcgtacct tcataattaa
     8581 cttgatcgaa tattttttta ttgtgcgccc atagataggg attggctgcc aggtgaaata
     8641 agcgatccac cccttttaat gcaaagcgga cagtgtccac gtcagtgata gatccttgaa
     8701 tcacttctac ttctttggga aaggttgtgt cagaaatttc cagcaccttg acggcctcgc
     8761 cttggtctaa taattgtttg accaaatgca tgccgataaa tccaccccca ccggttacca
     8821 atgagcaagt tttattattc aaaataaatc ctctttaatt caattacgtg tacaaaatca
     8881 gatgatccac acgcagaact ataaattatt tgtgaatttt ctaaaactgc ttatattaag
     8941 tgatcgtcga tattagtaaa tcaatttcta ttactgactg taatattaga aacaatctat
     9001 caatcagact aaaaccttac tggatagtat ttgttgcaag ttaaattttt cgatccccat
     9061 gaacgacttc aaccaatttt aatgcttgca attccatttc caagactaat gcatcaacct
     9121 ttgcacggaa gtgtctataa aaaatcatac ttggaatagc aactagaatt ccaaatgccg
     9181 cattatataa agcaatcgaa atcccatgag caagttgagc tggattgcta ccggttggtg
     9241 aattagaacc aaaaatttcg atcatcccaa taagagttcc aaataacccc atcaatggac
     9301 tcaaggatgc aatcgttccc aaggtagtca aatatcggtc caattcatgg gcgacgacac
     9361 gtcccgcttc ttcaatcgat tctttcatca cttcacgcga actttcgata tttttaagac
     9421 cagccgccaa gacacaacca agcggagagt tttcttgcaa tcgattcaac gtactcgcat
     9481 tgatacctga ccggcgataa tcatgcaaca ctttggataa caaatctcgc ggcgatacct
     9541 tacttgatcg caaactccaa attcgttccc caataattgc agccgctacg atggaagcaa
     9601 taataattgg ccatattggc caccccgcag cctgaatgat cgataacaca aaaaaatctc
     9661 ctgatgaaaa aaggttctat caagcaaata ctgactattc taacttgcct ttgcaatgaa
     9721 ctctactttt actatccaaa tgttatcgat ttgatagggt atgaagatat caatccgata
     9781 ccatccatac ccccaataag aaagcttaag actacattac caccagcgcg agatatcacc
     9841 accgaaaatg gccaccacga aaccctcctc gatgtccacc gataaaatgt ttatgaccgc
     9901 cgccaaaatg acgatgacca ccgatgaaat gaccatggcc acgattatag taccgcggtg
     9961 ttacgtacac ttggcgatgg ctcgtgtaat aacctggact aatggctacc ggcggacgat
    10021 agccataact aaaagaaaca ttgccatatc gcccattcac acccgagaag taaggagttg
    10081 ttacgcaccc actcaataac agcactccaa ctaacgctaa ggttaaggtc aattttctag
    10141 tagtcatgat tcatctcctc aaaacgcaag ggttaatcga gtaaccctta aactcactgc
    10201 acttattatc ctgatcctct caggatttag agatattgtg agtcgcttga gctgaatatc
    10261 acctgaataa gcaattcaag tgatgaaaag atttttgcac taaatgttga tatttattta
    10321 gtaaacttca gttggcttgt aggcatgcca taacgtaatg gtgccaccca acgtacggaa
    10381 atgaagcgac attggcatta aatttaaacc cgcttcttga atcaacttgg gtaaacaccc
    10441 atggatatga ctgtgaattt cctcaaacca acgaacaatc aatgaaatca cccacatgga
    10501 catatcatgg ggttcaccaa aatcaatgag atgtaatcga cctccgggtt tcagaactcg
    10561 aaaggcttct tctagcattt tttgcttaaa tacaggtttt aggtgatgca acagtaagct
    10621 gctaataata tgatcaaaat ggttatcagg ataaggtaaa gcgtttgccg atgcttgctt
    10681 tagcaaaagc tcaatatggc tttgttcgat ttttcgctgc gctagcttta acacctctgg
    10741 atcgatatct acaccatgaa ctatcgcttc aggttgagct cgcttgactc ggatagtatg
    10801 ggtaccagtt ccgcatccta catccaatac acgttctccc gattggatat ttgcttggcg
    10861 aattagcatg tcggttatcc gtgtttcagg gaatatcgtt cgcatcacgg gatcatacca
    10921 acgcgttaac cagtgataat gtaaagcagg tatgaaatct ggctgtttct tcatcgtagg
    10981 attaaggttt catttcaaga ttgatttatc aagccaaccc aagcttttcg catgcaggct
    11041 ctgaatatac aaccgctcat tcgtctcggt aacaccagtt gtttcaggat agtcaccggt
    11101 tgggtcttga agatccacaa caatctgccc ggtttcagta aatgcaatca catgcccata
    11161 ggattcgggc attggccata aaaagcgcgg aaaccgtaaa accatcttgc gcaatagcgg
    11221 tttatcgctc ataaaatcaa tggcggtatt gcgtggttta gctaagccca gccaaattct
    11281 tccctccagt ccacgcatta aattatccgg ataccctggt agattatcga gcaacacgga
    11341 agcatcagga gattcgtcac caatcgtaat ggcattcgca agtattgcaa ttttccatag
    11401 gcggtaacgc cccgtctcaa cgacaaacaa tgtttgctca tcgtgcgata gcgcgattcc
    11461 attggcaaaa ctcaaccctt gtgcaatgac tctcgttcta cctgacagtg aatcataggc
    11521 aatcaccctc cctgtggcag actgttcgac aatgtccagc attgtcgctt cgaaagttcc
    11581 atcccaatca gcgggtacaa aacgactgga tgaatcggta aaataaatcg taccgttact
    11641 tgcaaccacg acactattgg gataacgaat aggcttgcct tccacttcat cgaccaatac
    11701 ctcgatacgc cgatcagggg taatttttaa aacccctttc aaggcatccg ctgcgatgat
    11761 gttacccgaa acatcaaaat cgaatcccaa aattctaccg ccggtattca caaacacttc
    11821 taaatcgcta ccatccggat tcaaacgaag taaacttcca tccacgacgg ctgtatacaa
    11881 ttgaccggtt ggtgagatag caacatgctc tggcccctcg tagccctgta aatggatttg
    11941 atttaattgt cccaatcgct gatttaccgc atgctgatcg gtataaccgg gtgcactcgg
    12001 tgccttccaa ctttcgggtt caatcggaac tggccaactt agcgaataaa gcattgataa
    12061 caagacgatt attaataaaa tcttcattgc tttttcatcc catcactact aaaaaatatt
    12121 gacacaccca ttaaaatgta caaaagttca acagttctga aaagcaatca ctagaaaata
    12181 accacactca attttatttc attcaatttt gatatgctgc gtaaaatttt aattgcgaat
    12241 cgaggggaaa tcgcggtacg cattattcgc gcttgtgcgg aaatggggat acgctcggtc
    12301 gccatttatt ctgaggcaga tcggttcgcg cttcatatca agaaagccga tgaatcccat
    12361 tatatcggta gcgagcccat ggctggctat ctcaatattc atgctttggt tgatttagcg
    12421 cttgctactg gctgcgatgc cattcatccg ggctatggtt ttctctctga aaatgctgaa
    12481 tttgctaaag tgtgcaaaga acggggtttg gttttcatcg gcccaacccc agaagtcatt
    12541 caccgcatgg gtgataagac cgaagcacgc aatgccatga aagcagccgg tattccgatt
    12601 acaccgggtt cagaaggcaa cgtaaattct gtcaaagaag cgctcaacgt tgccgagcaa
    12661 attggctatc ccgtgatgtt gaaagccacc tctgggggtg gtggacgtgg tatccgtcgc
    12721 tgtgacactg cacaggaatt agaacgcaac tatacgcgcg ttatctccga agccaccaaa
    12781 gcgtttggtc gcgccgaagt ttttctggaa aaatgtatcg tcaatcccaa acatatcgaa
    12841 gtccaaattc tcgcggatca tcatggaaat gtgattcatc tctatgaacg tgattgctcg
    12901 attcagcgcc gtaatcagaa gttaattgaa attgcaccat ctccccaact cgatgaagcg
    12961 cagcgccaat atatcggtgg gttagctgtc atcgcggcta aatccgttgg atacactaac
    13021 gcgggtaccg ttgaattttt gctggatgat agtggccggt tttatttcat ggaaatgaac
    13081 acgcgcgtgc aagtcgaaca caccattact gaaacgatca cgggtgtaga tatcgttgaa
    13141 gaacaaattc gtgttgcagc cggattaaaa ttgcgcttca agcaagatga aatcgtgcgt
    13201 cgcggttacg caatccaatt ccgcatcaat gcggaagatc ccaaaaacaa ctttctaccg
    13261 agttttggac gtatttcgcg ttactatgca cccggtggcc ctggcgttcg aactgatacc
    13321 gcgatttata ccggctatga aattccgcct ttctatgatt ccatggtggc caaagtgatt
    13381 gtcagcgccc ttacttggga agatgtcatc aaacgcggcg aacgtacgct acgagatatg
    13441 gggttatttg gaatcaaaac gaccattccc tattatttga aaatcttaaa ccatccgcaa
    13501 tttcgtgcag cgcgcttcaa taccagtttt gtcgaacaaa atccacaatt gatcaattat
    13561 tccaataaac ctcgccctga agtgctagca agcgtcattg ccgctgcggt tgcatcgcac
    13621 accgggctct aatctattca aacgctatgc gccaagaaaa atccaagcaa accgttcata
    13681 tcaccgacgt cattctgcgc gatgcacacc aatcgttgat tgccacccgg atgcgtacgg
    13741 aagacatgtt accagcctgc accctgctcg accaaatcgg ttattggtcg ttggaatgct
    13801 ggggaggtgc aacgtttgat acgtgcttgc gttttttaaa agaagatccc tgggaacggt
    13861 tgagccaact caaagcagcg ttacccaata caccactaca aatgttactg cgcggccaga
    13921 atttgctggg ttatcgccat tactccgatg atgttgtgca tgcgtttatc caacgcgctg
    13981 ctaccaatgg aatcacgatt ttccggattt ttgacgcgct caacgacgta cgtaacctca
    14041 aaaccgccat cgaagcagtt aaatctgccg gtaagcatgc gcaaggtacg atttgctata
    14101 ccaccagccc agttcatgat gttaaaagct ttgtcagctt ggctagcgat cttgctaaac
    14161 tcggttgtga ctcgattgcc atcaaagata tggcagggct acttaccccc tttgcgacac
    14221 aggaattggt tagcgcattg aaacagactg tcgatttacc gattcatctg cattcgcatg
    14281 caacatcagg gctagctgaa atgtgccaac tcaaagccat cgaggccggt tgccgccata
    14341 tcgatacggc gctttcttca tggtccggtg gtaccagcca cccgccgact gaaagtttag
    14401 tgatggcatt acaaggtacg ccctacgaca ccaagctcga cctgcaaaaa ttgcaagccg
    14461 tgaatgatta ttttgcgcaa atccgtaaaa aatatcatcg atttgaaagc gaattcacgg
    14521 gagttgatac gcgtgtccat gtattccaag ttccgggtgg tatgatttcc aatttggcca
    14581 atcaactgca agaacgcaac gcgctagatc gtatcaatga agtctatgcg gaaattccac
    14641 gagtccgcaa agatttgggc tacccgccgc tggtcactcc gacttcgcaa atcgtcggca
    14701 cgcaagccgt cctcaatatt ttgaccggaa aacgctattc caccatcacc aatgaagtca
    14761 aacgctattt acaaggcggg tatggcaaag cgcctgctgc catcaatgcc agtttgcaaa
    14821 aaagtgcgat tggtaaggaa gatttgatcg attgccgccc ggctgatttg ttaaaacctg
    14881 aattcgagca actgcgcaag gaaatcggac agcttgcctt aaacgatgaa gatgtcctca
    14941 gttatgctat gtttccggaa atcggcaagc aatttctgga acaacgttca tcgggtcaac
    15001 tggttcctga accccttgaa gtggcttcga caaccgtcaa tggtgtgcaa aaagcaccga
    15061 ctgaattcaa tattgcgctg cacggtgagt cctaccatat caaagtcaca ggtaccagtc
    15121 ctaaaaccga aagcacgcgc catttttatt tcatggtcga tggcattccc gaagaaatct
    15181 tggttgaaac gctcgatgaa atcgtactcg atggcggcac tcaaggccct gtgaaaagcg
    15241 caattgctag caagcgccgt agccctacag aaaaaggtga tgtggtggtc agcatgccgt
    15301 gtaatattct cgatgtactc gtcaaaatcg accagaaaat caccgcggga caaccggtat
    15361 tagtgactga agccatgaaa atggaaacgg aaattaccgc accgatcagc ggtattgtca
    15421 aagccgtgca cgtggtcaaa ggtgaagcag tcaaccccaa tgaagtgcta attgaaatcg
    15481 tggcaggata agcttgctag aatttccaat aaattaaccc taaaaaatta cccgcagggg
    15541 ctccgcccct gcaccccgca aagggcaaaa gcacaaagaa caaacctcaa aaatccctgg
    15601 cgacgcggaa gccggaaaag tcgctcgacc caacggtaag gccccagtcg cggaaagccg
    15661 aacgcaggta ctgagtcggt cctgcaaaat taacttaacc gtggcggagg ttttgtatac
    15721 aagaaagt
//
LOCUS       DAOQKQ010000048        15646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_1735_length_15646_cov_6.253929, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000048 DAOQKQ010000000
VERSION     DAOQKQ010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 15646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15646
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1735_length_15646_cov_6.253929"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PKZ49_04655"
     CDS             complement(<1..370)
                     /locus_tag="PKZ49_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HND35887.1"
                     /translation="MSYQVIVKPSNHTFTIETGESILQAALRQGISLPYGCRNGSCGA
                     CKGKILQGHVDYGRYDETTLTEQDKQNGLALFCCASALSDNLVIECHEVDTLKDIRIR
                     TMPCRVHKLDRVAPDVMVIYL"
     gene            409..1314
                     /locus_tag="PKZ49_04660"
     CDS             409..1314
                     /locus_tag="PKZ49_04660"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HND35888.1"
                     /translation="MKKTLLIIGCGDIALRTAPLLARYYRLLGLTRRTENFSKLRSHQ
                     ITPLLGDLDQQASLKRLSGIADAIIHLAPPPNMGLKDTRTQHLLAALSRQHSRAKGRI
                     PHRLVYISTSGVYGDRDGQWVGEHNPVKPRNDRAWRRFDAECQIRKWGTDTGSNVTIL
                     RVPGIYAIDRLPIARLKQGTPALLEKEDSYTNHIHADDLARIIVAAFRYGKNSRIYNA
                     CDDSSLKMGDYFDLVADTFKLPHPPRISRTQAKEQIPAGLLSFMEESRRLTNDRMKRE
                     LRIKLLYPTVHEGISKVIPDKNCLR"
     gene            complement(1392..2597)
                     /locus_tag="PKZ49_04665"
     CDS             complement(1392..2597)
                     /locus_tag="PKZ49_04665"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HND35889.1"
                     /translation="MLNLTSIQETLLPSVNPDINSPLTDSAGLNQSNSTPFQEILGKT
                     LAEDLKTELSADITDTETAISNELLIEATNAVEIPISLMAETNTANSNSMTSFSLADS
                     SNILRGAQNQINPSLLNTIEENILQITPLLDTALENSDAPLATLISDNLKTTISTSSA
                     SNQTYTTFLQAQAYQNNSIPSINTSPYSALNQFTQLLTRDASSEEVDITPVNFAEIGK
                     ILPISPDSSLVAQSNLITQTVSPSSSTPLLPIHPHHVATEFGKPAWGNDFNQQITWLA
                     TQKHQIAELHLHPAELGPVEVILHISNDQSTQVSAQFTSPHLAVREAIESALPRLREM
                     MAENGITLGNTTVGAETSQQHANQQHGSIASNANENSTTTVATIQGLSTEFNGSVLPR
                     HQGIINTFA"
     gene            complement(2672..3118)
                     /gene="fliJ"
                     /locus_tag="PKZ49_04670"
     CDS             complement(2672..3118)
                     /gene="fliJ"
                     /locus_tag="PKZ49_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HND35890.1"
                     /translation="MATTQSLKTLVEFAQQKMDASAKKLGFLNSKQQEAEKKLNLLID
                     YRRNYQAHFVNTKNTGMNSIEWTNYLAFIGKLDAAIVEQRKVTINAQKETEIVKTEYQ
                     DSRRKLKSYHTLLQRIKHHHAIKLEKHEQKLLDEFVTIARQHKTEG"
     gene            complement(3159..4556)
                     /gene="fliI"
                     /locus_tag="PKZ49_04675"
     CDS             complement(3159..4556)
                     /gene="fliI"
                     /locus_tag="PKZ49_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HND35891.1"
                     /translation="MNHCSSWQSFLNNCKQIVSHSNSLIISGTLTRVAGLVMEAVGLR
                     LAVGSTCTVFLPNGHNVSAEVVGFSGDRLFLMPSSDVYGLVPGAKVVPTEYASDHPRI
                     GKNWRPRRRAADRTKQINVGYELLGRVLSGAGEPLDQLGIIHTEQTVPLYSRPFNPLE
                     RAPIDMPLDVGIRAINTLLSVGRGQRMGLFAGSGVGKSVLLGMMARYTKADVIVVALI
                     GERGREVKEFIENILGQEGLARSVVIAAPADTSPLMRLQGAAYATAIAEFFRDEGKNV
                     LLIMDSLTRFAMAQREIALAVGEPPATKGYPPSVFARLPQLVERAGNGRQGSGSITAF
                     YTVLSENDDPNDPIADNARAILDGHIVLSRRLADSGHYPAIDIESSISRVMPHLASQE
                     QLEWTRRFKNLYARYHRSHDLISVGAYVPGSDPELDLAIKIYPALESFLQQGIFEHEN
                     IAESLQKLSKLLTLR"
     gene            complement(4564..5229)
                     /locus_tag="PKZ49_04680"
     CDS             complement(4564..5229)
                     /locus_tag="PKZ49_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HND35892.1"
                     /translation="MNSPSVIPKKQLSSFQKWELGSFETNLANNTTQLDESVDQVAKE
                     TISLPTAEQIEAIYQQAKNEGYQAGYQEGKQHATVEMETTIQHLQVLMVKFEEELQHI
                     DQTVAQELLELAIEFARKIVTQSLELKPELIIPIIQDAIQHLPSAMQPLHIILHPEDA
                     TLVRIQLENQLSHSKWKIYENSQIERGGCKVEAGGCEIDATLASRWKTILSKVLLENH
                     QPN"
     gene            complement(5325..6323)
                     /gene="fliG"
                     /locus_tag="PKZ49_04685"
     CDS             complement(5325..6323)
                     /gene="fliG"
                     /locus_tag="PKZ49_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HND35893.1"
                     /translation="MSDDGILKSAILLMSLGEEEAAEVFKYLGPKEVQKLSEAMSSLN
                     NVKRDKIESVVHEFCTLASDRTSLGQNSLAYIRNVLIRALGEEKAASLLDRMTEKGND
                     TSGIENLKWMDTAAVAELIKNEHPQIIATILVHLERDQASAILALFTERLRNDVILRI
                     TTLDSIQPTALRELNSVLNKLLSGSDNIKKAPKGGIRTTAEMLNFIPSSLEGNIIEHI
                     TQYDESIAQKIMDEMFVFDDLLTVDDQGIQLLLREVQSDSLIIALKGAKEELRNKIFK
                     NMSQRAAEMLREDLEIKGPVRVSEVEAAQKEMLKTLRQLADDGKVALGGKGGDGFV"
     gene            complement(6313..7956)
                     /gene="fliF"
                     /locus_tag="PKZ49_04690"
     CDS             complement(6313..7956)
                     /gene="fliF"
                     /locus_tag="PKZ49_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HND35894.1"
                     /translation="MATVSEEIGTKTNRFSAIAQLSGQKKLGLMLAAAASIALLVGIA
                     IWSQSPDYRVLYTNITEQDGGAIINALQQMNIPYQFSEGNGTILIPGTQVHEVRLRLA
                     SQGLPKGGISGFEILENQKFGSSQFLEQVNYQRALEGELARSIQSLSAVQSARVHLAI
                     AKPTVFVRERQQPSVSVLLNLYPGRVLTTEQVSAIIHLVSSSIPSLPVKNVTVVDQNG
                     NLLSTPKKEGNETGLDDSQLKYIHELEQNINKRIETILLPITGPNNVRAQVTADVDFS
                     RTERAEEIYKPNNIATEAASIRSQQKSDSMSANRPESGVPGALSNRPPAAAEAPVDVA
                     AGEGDTKNQVPVDTRSESTTNYELDKTIQHTRQSVGRINRLSAAVVINYRGKMDEAGN
                     LIHEPLAAEEIEKINSLVKQAMGFDENRGDTLTVTNSQFNILEEEIAEIPFWKQPDMI
                     LLAKEIGKQLIIAALVLFFLLKILKPFLKTLLPPPPPPPPAPLQATAGLEESNNLALS
                     HQQEVVSHEQNITRAKQLALNEPIIVANVIKEWVSDNER"
     gene            8188..9411
                     /locus_tag="PKZ49_04695"
     CDS             8188..9411
                     /locus_tag="PKZ49_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HND35895.1"
                     /translation="MKAESNVRLGKSDADHHQLMHAFTVFSQASEQLSTVYRDLQQQV
                     EHLTNELALANGELQHQLAQKENLSQQLSLLLSALPGGVVAIDNEGVIESANPVALSI
                     LGEPLLQCTWQQILEDRLLATDVANEWNLRKETHSDFRRISIESSASEDSGKMVLLIQ
                     DITEAYTNQERIKREQRLAVMGRMAANLAHQLRTPLSTALIYATHLSNSELPTQERSS
                     FAVKTIERLQYLNQLINSMLRFVKGELVKKEVIDISVLLSDIQSLMLPHMLKKNLQFV
                     VKDGGEGAALTVNYEELCSSLIALLENAMQASPVGGRISLTCDLTTDEVFFTVADQGN
                     GIDIANEERLFEPFFTTRPEGTGLGLAIVHSAIKQMGGDVQVKLLTDRGSEFILRLPR
                     TSLSNITNSNLSSSI"
     gene            9424..>10123
                     /locus_tag="PKZ49_04700"
     CDS             9424..>10123
                     /locus_tag="PKZ49_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HND35896.1"
                     /translation="MNELSILIVEDDQDLLEAICTTVKLSGYKALGASNGTAALDLLN
                     SQQIGLVISDVQMQPIDGNILLKRIKETYPDIPVLLMTAYGEINKAIAAMRAGASDYL
                     LKPFSPDYLLTYIKNYLVPANNQRNNYEEVIAKDPRTSAMFSLAKRVAESSATVMLIG
                     ESGSGKEVLARYIHQHSPRLGKPFVAINCAAIPENLLEATLFGYEKGAFTGAAQAQPG
                     KFEQANEGTLLLDEI"
     assembly_gap    10124..10223
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10224..10904
                     /locus_tag="PKZ49_04705"
     CDS             <10224..10904
                     /locus_tag="PKZ49_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HND35897.1"
                     /translation="QPGKFEQANEGTLLLDEISEMPLGLQAKLLRVLQEREVERIGGR
                     KPVKLDIRLLTASNRDIFSLVKQGAFREDLYYRLNVFPLEIAPLRERPLDIEPLVHRI
                     LQSPNKGIKGRNYSLTKLALLALRQYSWPGNVRELENVIQRAMILATDEIDVMHLNLP
                     EQARSDVTLPFDTSDLSQSKDIKSLEREHILRTLAEVNGSRKLAVQRLGISERTLRYK
                     LQQYRLVG"
     gene            10925..11251
                     /gene="fliE"
                     /locus_tag="PKZ49_04710"
     CDS             10925..11251
                     /gene="fliE"
                     /locus_tag="PKZ49_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HND35898.1"
                     /translation="MDKSNINQILTQLRLTSDAISGSLKPDNAQFNPKEADFSNILKS
                     AVDQVNATQQTAGQLSQEFMKGGSDTNLHDVMISLQKANISFQSMIQIRNRLVTAYQE
                     IMNMQV"
     gene            11280..12008
                     /locus_tag="PKZ49_04715"
     CDS             11280..12008
                     /locus_tag="PKZ49_04715"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HND35899.1"
                     /translation="MNINQDYLIDSQNEVTHILRTFLEQQTKVLLYLIGENISPVPTT
                     VLNINPSQDELIVGYYPEAYFNQRAQLAERLVCVATHNGIRVEFESFSIGKTWFGRDA
                     AYRLDFPKVISRVQRREYFRMSPLMKQQPRCAILDKDQQVIGFTILDISCGGMSIKHD
                     RPNIKKLEVGEIYPDCSIELASPIGLIKTPIRIRNISEMPSKSNQIYTRYGCEFIDLP
                     SKTRMMIQRYIIKLEQDSRSKDWM"
     gene            12180..13832
                     /locus_tag="PKZ49_04720"
     CDS             12180..13832
                     /locus_tag="PKZ49_04720"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HND35900.1"
                     /translation="MKSIKTKFVTFAIIAISIPSLSLGLLSYFKNEGLVRNHVTRELR
                     VLASNVSRELDLWINENYHAVSVLSVSNLIINELALSARDSVSKSSNHQGSRQLVLSK
                     YLSSFQEKLPTIVELTLFDLNGNVVASSIPESLNSVIRGNEQHDMLTSNIKIKPPYRS
                     DRYDTAVLELYSPVLSYDEKVIGSILATLNMEKFMSSLVDNTDFSLGEITLLGYAGNT
                     ILSSNHKINYSASLDSKTLKQLQDYGGEALAFKGLTHSKVIGLISKSKEAPIIVLVER
                     DYAEAIAAWIELRNLFMSLVGLLIVIVGGIAFYIGHSIVASLSYLIEGTKRILNGDLN
                     VQINEIKADEIGQLTASFNKMTENLRCSQAEILAAHEAMQKQNQQLKVLSITDSLTEL
                     YNRNKLDAILIDQIARFERNHRPFSVVMMDIDHFKTLNDNYGHVIGDEILVSVSQAIS
                     KSIRSVDFAARYGGDEFVVILTETDAASAVITAERIRAQVAINVNSQIKEVSLSITLS
                     MGIIQSEIDDKTPKNILSRVDGALYKAKNSGRNRIYCAHHPS"
     gene            complement(13851..15440)
                     /locus_tag="PKZ49_04725"
     CDS             complement(13851..15440)
                     /locus_tag="PKZ49_04725"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HND35901.1"
                     /translation="MKNSKTLTEIEIAREALRQLAVNRKLPTPDNYRSLYYQIAGVEI
                     IDFFPEKTLKIIIEKLPRNNSDQQQIIQSFEAAVSKGDWESLGAILIDLLSGNVIKQN
                     PISNDHSNDHHLEKDNVQPNETGKLITSILPRELQDQLGRLFGDCLSILLSTSPEISK
                     DAESLAREVRVISESGQLSQFTDKLKQFNYQLQYAAEDQGELRTSLLKLFQLVIDNIS
                     ELVVDDAWLQGQIAIVREMITEPLDLRLIDNVERQIKELIYKQGLLKKNLIDNQSRLK
                     LMLSSFVDRLAEFTQTTGDYHASLGVYAEEISQAEDISELSDVLDRIIQQTRQVQSSA
                     KRSHDELLELRSRAESAEKEVDRLQSELLHASELIRHDALTGALNRKGLEEAIEREVS
                     RKYRDGSSLCIALLDIDNFKKLNDTHGHEAGDDALVHLAAVVRKSLRPQDTLARFGGE
                     EFVVLLTGTGVNDSVTVMQRVQRDLTRCFFLHQNEKILITFSCGIAELAENEDPKTTI
                     KRADQAMYLAKRSGKNRVLAA"
BASE COUNT         4353 a         2990 c         3379 g         4824 t
ORIGIN      
        1 tgagataaat aaccatcaca tcaggtgcta cacggtcaag tttgtggacc cgacagggca
       61 tggtgcgtat tctgatgtct ttaagggtat cgacctcgtg acattcgatc actaagttgt
      121 ccgataatgc cgatgcacaa caaaacaaag ccaagccatt ttgtttgtct tgttcagtga
      181 gtgttgtttc atcatatcta ccgtaatcga catgtccttg caaaattttt cctttgcaag
      241 cgccgcatga accattgcgg cagccgtaag ggagtgaaat gccttgtctt agagctgctt
      301 gaagaatcga ttcccctgtt tcgatagtaa aagtatgatt gcttggcttg acgatgactt
      361 gataagacat agtttaaaaa tttaaaacga ttaatttgat acgctgaaat gaaaaaaacg
      421 ttactgatta ttggatgtgg ggacattgca ctcagaacag ctccgcttct cgctaggtac
      481 taccgattgc ttggattgac tcgtcggaca gaaaatttta gtaaactacg ttcacatcaa
      541 atcaccccat tgttgggtga tttggatcag caagcaagcc tcaagcggtt gtctgggatt
      601 gcagatgcca ttattcatct ggcgccacct cctaatatgg gtttaaaaga tactcgtacg
      661 cagcatcttt tggcagcgct gagtcgacaa cattccagag caaagggaag gataccacat
      721 cggctagtgt atatcagcac aagtggagtt tatggtgata gggatgggca gtgggttgga
      781 gaacataatc ccgttaagcc acgtaatgat cgagcttgga ggcgatttga tgcagaatgt
      841 cagattagga aatggggcac cgatacaggt agcaatgtca ctatattgcg ggtgccgggc
      901 atctacgcta tagaccgatt gcccattgct cggctaaagc aaggtactcc agcattacta
      961 gagaaggaag atagttatac taatcacatc catgccgatg atctggcgcg gattatcgta
     1021 gctgctttcc gttatgggaa aaatagtaga atttataacg catgtgatga ttcgagttta
     1081 aaaatgggtg attattttga tttggttgcg gacactttta aattacccca cccacctaga
     1141 atttcgcgta cacaagccaa agaacagatt ccagccggcc tgctatcttt catggaagaa
     1201 tcacggcgtt taacaaatga tcgaatgaaa cgagagttgc gcatcaaatt gctttatcca
     1261 actgtgcatg aaggaatatc caaggtgatc cccgataaaa attgcttgcg ttaatttgaa
     1321 ataccgtgct atcaggttgt ttgagcgggc gagcttggct attcctcaat tctactcaaa
     1381 ggtgtttcga gtcatgcaaa tgtattgata ataccttgat gccttggaag aacactgcca
     1441 ttaaattcgg ttgataatcc ctgaattgtt gctactgttg ttgtgctatt ttcattggca
     1501 tttgaagcaa ttgaaccatg ctgttgatta gcatgttgtt gagatgtttc agcaccaacc
     1561 gtggtatttc ccagggtaat tccattttct gccatcattt cacgtaatcg aggtagcgcg
     1621 gattcaattg cctcgcgtac cgcaagatgc ggagaggtga attgagcaga tacctgagtc
     1681 gattgatcgt tactgatgtg caaaatgacc tctaccggac cgagttcggc cggatgtaga
     1741 tgtaattccg cgatctgatg cttctgggtg gctaaccagg ttatttgctg attgaaatcg
     1801 ttgccccatg ctggcttgcc aaattcagtc gcaacatggt gtggatgaat tggtaataag
     1861 ggagttgagc tggaaggaga caccgtttga gttatcaagt tgctttgtgc taccaaagaa
     1921 gaatcgggag aaatcggcaa aattttgccg atctcggcaa aatttaccgg ggtgatgtcg
     1981 acttcttcag atgaagcgtc acgtgttaat aattgggtga actgattcag agcagagtag
     2041 ggcgaagtat ttatactcgg tatactattg ttttgataag cttgggcttg caaaaaagtt
     2101 gtgtaagtct ggttgcttgc tgagctagta gaaatggtcg tctttaaatt atcacttatt
     2161 agcgttgcca atggcgcatc gctattttca agcgctgtgt caaggagtgg cgtgatttgt
     2221 aaaatatttt cttctatcgt attgagtagc gatggattga tttgattttg tgcccccctc
     2281 aggatattgg aagaatctgc taatgagaag ctagtcattg aattgctgtt agcggtgttt
     2341 gtttcagcca tcaaggagat aggtatttcg actgcgttgg ttgcttcaat aagtaactca
     2401 ttcgatatgg ctgtttcagt gtcagtaatg tcggcgctaa gctcggtttt tagatcttct
     2461 gctagggttt ttcccaatat ctcttggaat ggggtcgagt tagattgatt tagaccagcg
     2521 ctgtccgtga gcggtgaatt aatgtcggga ttaacagatg gcagtaatgt ttcctgaatg
     2581 cttgtaagat ttaacatggc taataggtga gttactaaat taatatctat cctcatgcaa
     2641 ttgttatgcc aggatcagat tttcttgggg cctagccttc tgttttatgt tgcctggcaa
     2701 tagtaacaaa ctcatccagg agcttttgtt catgcttctc aagcttgatg gcatgatgat
     2761 gtttaattct ctgtagcagc gtatgatagg actttagttt acggcgagaa tcttgatact
     2821 ctgttttaac aatctctgtt tctttttgtg cattgatggt tactttgcgc tgctcgacga
     2881 ttgcggcatc cagtttgcca ataaatgcaa gatagttagt ccactcaatt gaattcattc
     2941 cagtgttttt tgtattgaca aaatgggctt gataatttcg tcggtaatca atcaatagat
     3001 taagtttttt ctcagcttct tgctgtttgg aattaagaaa tccgagcttt ttggctgaag
     3061 catccatttt ttgttgagca aactcgacaa gcgttttaag agattgggta gttgccattc
     3121 ggtttgctat ttactatggg tagtgattca aaagcgagct accgaagtgt gagtagcttg
     3181 gataattttt ggaggctttc tgcaatgttc tcatgctcga aaatcccttg ctggaggaaa
     3241 gattctagtg cgggataaat ttttatcgct aaatcaagct cgggatcgct tcctgggaca
     3301 taagcgccga cactaattaa gtcgtggctg cggtgataac gcgcataaag attcttgaaa
     3361 cgccgagtcc attccaattg ttcttgtgaa gctaaatgcg gcataacacg gctgatcgat
     3421 gattcgatat cgattgccgg ataatggcca gaatccgcta atcgccgtga gagaacaata
     3481 tgtccatcga ggatggcgcg ggcattgtct gcgattggat cattgggatc atcattttct
     3541 gataggacgg tataaaatgc agtgattgag ccactacctt gtctaccatt tcctgcacgt
     3601 tccaccaatt gtggtaaacg cgcaaatacc gatggtgggt aacctttagt ggcgggaggc
     3661 tcaccgactg ctaatgcaat ttctcgctgt gccatggcaa accgagttag cgaatccatg
     3721 atgagtagta cattttttcc ctcatcacgg aaaaattctg cgatagcagt tgcataagcc
     3781 gcaccttgca agcgcataag gggagaggta tcagctggcg ctgcgataac aactgaacga
     3841 gcgagccctt cttgtccgag tatgttttca atgaattctt ttacttctct gcctctctcg
     3901 ccaatcaacg ccacgacaat cacatcggct ttggtgtacc gtgccatcat tccgagcaaa
     3961 acacttttac caacgccact tcctgcgaaa agtcccattc tttggcctcg gcccacgctg
     4021 agtagcgtat taatagcgcg aatgcctaca tcaaggggca tatcaatggg ggccctttct
     4081 agtgggttga atggtctgct atataaagga accgtctgct cggtatggat aatacctaat
     4141 tgatcaaggg gctctccggc accactcagt accctcccta atagttcata tccaacatta
     4201 atttgctttg tgcggtcggc agctcgcctt cttgggcgcc agtttttgcc gattctggga
     4261 tgatcactcg cgtattcggt gggaactact ttcgccccag gcactagtcc ataaacatca
     4321 ctagaaggca ttagaaatag gcgatctcct gagaatccaa cgacttcagc agagacatta
     4381 tggccattgg gaaggaaaac ggtgcaagtg ctgccgacag caagtctcaa tcctaccgct
     4441 tccatcacta gacccgctac acgggtcaat gtaccactaa taatgagtga atttgagtga
     4501 ctgacaattt gcttgcagtt gtttagaaaa ctttgccatg aggagcaatg gttcatgctt
     4561 accctaattc ggttgatgat tttctagcaa aacttttgat aaaattgttt tccagcgcga
     4621 agcgagtgtg gcgtctatct cgcaccctcc cgcttctact ttacatcctc ccctttctat
     4681 ctggctgttt tcgtagattt tccactttga atgcgataac tgattctcca attggatgcg
     4741 aaccagcgtc gcatcttcag gatgcagtat gatatgaagc ggctgcatcg cagatggcaa
     4801 gtgttgaata gcgtcttgaa taattggaat gattaactca ggttttaatt caagcgattg
     4861 agtaacgatc tttctcgcaa attcgatagc gagttcgaga agctcttgcg caacggtttg
     4921 atcaatgtgc tgaagctcct cctcaaactt gaccattaag acttggagat gttgaatagt
     4981 ggtttccatc tctacagtcg catgttgctt gccttcttgg tagcctgcct gatagccttc
     5041 attctttgct tgttgataga tcgcttcgat ttgttctgct gtaggaagtg aaatggtttc
     5101 tttagcaact tgatcgacgc tttcatctaa ttgtgttgtg ttgtttgcta aatttgtttc
     5161 aaatgaacct aactcccatt tctgaaagga cgaaagctgc tttttaggaa tgaccgatgg
     5221 ggaattcatt tgtatgttta tccttatcca atagttcgat atgaattagc aggtgtaggt
     5281 ccatgatcac ggtagtttgt tagattgaga gctatcttta gctgttatac aaaaccatcg
     5341 ccacctttgc ctccaagcgc tacttttcca tcatctgcta gttgtcggag ggtttttaac
     5401 atttccttct gcgcagcttc aacttctgaa acacgaactg ggcctttgat ttcaagatct
     5461 tcgcgcaaca tttctgcagc ccgttgtgac atattcttaa agattttatt acgtaattct
     5521 tctttggcgc ctttgagagc gatgatcagt gaatcagact gaacttctct gagtaagagt
     5581 tggatacctt gatcatcaac agtcagtaaa tcatcaaata caaacatctc atccatgatt
     5641 ttttgtgcaa tgctctcatc atactgtgtt atgtgctcga taatattgcc ttctagcgaa
     5701 ctgggaatga aatttagcat ttcagctgtc gtgcgtattc cccctttggg cgctttctta
     5761 atattgtcac ttccagatag caatttattt aatacactat tcaattcacg cagtgcagta
     5821 ggctgaatgc tgtcaagggt tgtgatacga agaataacat cattgcgtaa cctctccgta
     5881 aataatgcca gaattgcgct ggcctgatct ctttcaagat gaaccaatat ggttgcaatg
     5941 atctgtgggt gctcattttt gattaactct gctactgcag cagtatccat ccattttagg
     6001 ttttctatac cactagtatc gttccctttt tccgtcattc gatccagtaa gctagccgct
     6061 ttctcttccc ctaaagcgcg gattagaaca ttacgaatat atgctagcga attttgaccg
     6121 agcgaagttc tatcacttgc tagcgtacag aactcatgta cgacggactc aatcttgtca
     6181 cgtttaacat tgtttaaact tgacatggct tcactcaatt tctggacttc tttcggtcca
     6241 agatacttaa aaacttcagc tgcctcttct tctcctaatg acatgagaag aattgcgctt
     6301 ttaagaatac cgtcatcgct cattgtcaga tacccattct ttgatcacat ttgctactat
     6361 gattggttcg tttaaagcca attgcttagc tctagttata ttctgctcat ggctaactac
     6421 ttcctgctga tggctcaatg ctaagttatt actctcttct aacccagcgg ttgcctgaag
     6481 cggtgctggt ggaggaggtg gcgggggtgg caacaaggtt tttaggaatg gttttaatat
     6541 tttaagcaaa aagaataaaa ccagagcggc aataataagc tgcttaccaa tttcttttgc
     6601 aagcaaaatc atatctggtt gcttccagaa aggaatttct gcaatttctt cttccagaat
     6661 attaaattga ctattggtta cagtaagtgt atctccccga ttttcatcaa atcccatcgc
     6721 ttgtttaacg agactattaa ttttctcgat ttcttctgca gcaagtggtt catgtatcaa
     6781 gtttcccgct tcatccattt ttccgcgata attgatgaca acggctgcag atagccggtt
     6841 aatacgtccc actgattgac gtgtgtgctg aattgttttg tctaattcgt aattcgtagt
     6901 tgattcggag cgggtatcaa cgggtacttg attttttgtg tccccttcac cagctgcgac
     6961 atctacaggt gcctcagcag cagcaggtgg acgatttgat agcgctcctg gtacaccgct
     7021 ctcgggtcga ttagctgaca tggagtctga tttttgttga ctccgaatcg aagcagcctc
     7081 ggttgcaata ttattaggct tgtagatttc ttctgcgcgt tccgttctag agaaatcaac
     7141 gtcggcagta acttgagccc gtacattgtt gggaccagtt attggaagca aaattgtttc
     7201 aatgcgctta ttaatgtttt gctctaattc atgaatatat ttgagctgac tgtcgtctag
     7261 gccagtttca tttccttcct ttttgggagt gctaagtaaa ttgccatttt ggtcaacaac
     7321 ggtaacattt tttactggaa gacttggaat actacttgaa actaaatgaa tgatagcgct
     7381 tacctgttct gtggttagca ctcttcctgg gtataaattt agtaatacgg atacgctagg
     7441 ttgttgacgt tctcgaacaa agacggtggg cttagcgatt gctaaatgta cgcgagcact
     7501 ttgaacagca gaaagggact gaatagaacg cgccaactca ccttctaatg cacgttgata
     7561 gttaacttgt tccaagaact ggctggaacc aaatttctga ttttctaaaa tctcgaaacc
     7621 ggagataccc cctttaggaa gcccttggct tgccaagcgt aacctaacct catgaacttg
     7681 tgttcctgga attaaaatgg ttccattacc ttcagagaat tggtaaggta tattcatttg
     7741 ctgtaatgca ttaatgatag ctccgccatc ttgctcggtt atattagtgt atagcactct
     7801 ataatcgggg ctctgactcc agattgcgat tccaaccaat aatgcaatgc tagccgccgc
     7861 tgctagcatt aaacctagtt tcttttgccc tgataattga gcaatagcgc taaatcgatt
     7921 tgttttggtt ccaatttctt ctgatactgt agccacgcac aatcctccta actactttta
     7981 gttaccaatc aatgtgttaa atattgaata tagatgctgt ttaaaatgaa gtatcggtaa
     8041 cttaaattcg ataatgcgat tatcaggatt tttattactt ctgaatgatt gaacagaata
     8101 attttgctag ctttatttca tctctatgtg caaggtggct aatgctaagc tgcacaacat
     8161 ctggaaaaac cattaaattt tgtaagcatg aaagctgaat ctaacgttcg attaggaaaa
     8221 tcagatgctg atcatcatca gctgatgcat gcttttactg tttttagtca agcgtccgaa
     8281 cagctttcaa ctgtttatcg cgatttgcag caacaagttg aacatttaac gaatgaatta
     8341 gctttggcta acggcgagtt acaacatcaa cttgcacaaa aagagaatct ttctcaacag
     8401 cttagtttgt tgttgtcagc tttaccgggc ggggtcgttg ctatcgataa tgaaggtgtt
     8461 attgaaagtg caaatccagt tgctctgtct attctaggtg agccgttatt gcaatgtacc
     8521 tggcagcaga tactcgaaga tcgtttattg gcaacggatg ttgctaacga gtggaattta
     8581 agaaaagaaa cacatagcga ttttcgtcgt atttcaattg aaagcagtgc ctcggaagac
     8641 tcggggaaaa tggttttatt aattcaagac atcactgaag cctatacaaa tcaagaacgg
     8701 atcaagcgcg aacaaagact tgcagtaatg ggaagaatgg cagcaaattt agcacatcaa
     8761 ttgcgtaccc cgctttcaac ggcattgatt tatgctacgc acttgagcaa tagtgagtta
     8821 ccaactcagg agagaagcag ctttgccgta aaaacaattg agcgattgca gtatttaaat
     8881 caactgatta atagtatgtt gcggttcgtg aaaggtgagt tagtaaaaaa agaagtgata
     8941 gatatctcag ttttattatc cgatattcaa agcctgatgc taccccacat gctaaaaaag
     9001 aatttgcagt ttgttgttaa agatggtggt gagggggcag ctttaaccgt taattatgaa
     9061 gaactttgta gctcgctaat agcactactt gaaaatgcga tgcaagcttc acccgtgggt
     9121 ggacgtattt cattaacttg tgacttaact accgatgaag tgttttttac agtagctgat
     9181 caaggaaatg gtattgatat tgcaaacgaa gagcggctat ttgagccttt ttttacgaca
     9241 cgcccagaag gaaccggtct gggattagca atcgttcata gcgcaatcaa acaaatgggg
     9301 ggggatgtgc aagtaaagtt acttactgat cgaggtagtg aatttattct ccgattgcca
     9361 agaactagct taagcaatat taccaattca aatctatcca gttctattta gaggattatt
     9421 gaaatgaatg agctatcgat tctgattgta gaagatgatc aggatttact ggaggcaata
     9481 tgcacgacag ttaagttgtc tggttataaa gccttagggg catcgaatgg cactgcagcg
     9541 cttgatttac ttaattccca acagattgga ttggtaatta gcgatgtgca gatgcaaccc
     9601 atagatggga atatactgtt gaaaagaatt aaggaaactt atccggatat ccctgtgctg
     9661 ctgatgaccg cttatggtga aattaataaa gcgattgcag caatgcgggc aggtgccagc
     9721 gattacttgt taaaaccctt ttcaccggat tatttgctga cctacattaa gaattattta
     9781 gtacctgcga ataatcaacg caacaattat gaggaagtta tcgctaaaga tccccgaact
     9841 agcgcaatgt tttctttagc caagcgtgtc gcagagtctt ctgcaactgt catgttaatt
     9901 ggtgaaagtg gcagtgggaa ggaggtttta gcccgttata tccatcagca ttcaccgcgt
     9961 ttgggcaaac catttgtggc tatcaattgt gctgctattc ctgagaattt attggaagca
    10021 acattatttg gatatgaaaa gggggcattt acaggcgcag ctcaggctca acccggtaag
    10081 tttgagcagg caaatgaggg tactttattg ctggatgaga tatnnnnnnn nnnnnnnnnn
    10141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10201 nnnnnnnnnn nnnnnnnnnn nnncaacccg gtaagtttga gcaggcaaat gagggtactt
    10261 tattgctgga tgagatatct gaaatgccgt taggtttgca agctaaattg cttagagtgc
    10321 ttcaagaacg tgaagtagag agaattggtg gtagaaagcc cgttaagcta gatattcgcc
    10381 ttttaacagc atcgaatcgt gatatttttt ctttagtaaa gcaaggcgct tttcgggaag
    10441 atttatatta tcgacttaat gtttttcctt tagaaatcgc accgctgagg gaacgcccac
    10501 tggatattga acctttggtt catcgaatac ttcagagtcc gaataaagga ataaaggggc
    10561 gaaattactc tttgaccaaa ttagcattgc ttgcgcttag gcaatactct tggccaggaa
    10621 atgttcgtga attagagaat gttattcaac gtgcaatgat tttagcaaca gatgagattg
    10681 atgtaatgca tttaaattta cccgagcaag cgcgatctga tgtgacacta ccgttcgaca
    10741 cgagtgatct atctcagtcg aaggatatta aatctttaga gcgtgagcat attcttagaa
    10801 cacttgctga agtaaatggc tcgcgtaaat tggcagttca gaggctcggg atatctgagc
    10861 gtacattacg ttataaattg caacaatatc gcctagttgg gtgagaatcg ataggagggc
    10921 tgtagtggat aaatctaata ttaaccaaat cttaactcaa ttaaggctta cttctgatgc
    10981 aatttctggt tccttaaagc cagataatgc tcagtttaat cccaaggagg cagattttag
    11041 taatattctg aaatcagcgg ttgatcaagt aaatgctact cagcaaaccg cagggcaatt
    11101 gagtcaggag tttatgaaag gtggatctga tactaatttg cacgatgtaa tgatttcact
    11161 ccagaaagcc aacatctcgt ttcagtcgat gattcagata agaaatagat tggtaacggc
    11221 ttatcaggaa attatgaaca tgcaagtata aatcttaaat aaatgccctt ctgatctgaa
    11281 tgaatattaa ccaagactat ttgattgatt cccagaatga ggttactcat attctgagaa
    11341 cgttcctaga gcaacagact aaagttttgc tttatcttat tggtgaaaat atcagtccag
    11401 tgccaaccac agttttaaat attaaccctt cacaagatga gttgattgta ggttattatc
    11461 ctgaagcata ttttaaccag cgagcccaac ttgcagagag attagtctgt gtagcaacac
    11521 ataatggtat tcgagttgag ttcgaaagtt tctcgattgg gaaaacttgg tttggtagag
    11581 atgctgccta tcggctagat tttccgaaag taatttcacg tgtacaacga agagaatatt
    11641 ttcggatgtc gccgcttatg aaacaacaac caaggtgtgc tattttggat aaagaccaac
    11701 aggtgattgg ctttacgata cttgatatca gttgtggagg gatgtcgatc aaacatgacc
    11761 gtcctaatat aaaaaagctt gaagttggcg agatttatcc tgattgcagc atagaacttg
    11821 ctagcccaat tggccttatt aagacaccaa ttcgtattag gaatatttct gaaatgcctt
    11881 ctaaaagtaa tcaaatttat acccgatatg ggtgtgagtt tattgatttg cccagtaaaa
    11941 ctcgaatgat gattcaacgt tacatcatta aactagagca agatagcaga agtaaagact
    12001 ggatgtaata atcttgaaaa aagttatgca gtgtttttaa ctgctttgta cgattattgg
    12061 atctgaattt atatagctta gttaatcctg cccagtatct ctgatttgta atagtaaact
    12121 ttgttttttg tgttgctaga gaataaaatc tctttatagc gctattctta ggaaacagat
    12181 tgaaaagtat taaaactaag tttgttactt tcgcaataat tgcaatatca attccatcgc
    12241 tatcactcgg actactctcc tattttaaaa atgaagggct agtgagaaat catgtgactc
    12301 gtgaattacg ggtacttgct agtaatgtta gccgcgaatt agatttatgg attaatgaaa
    12361 attaccatgc cgtcagtgta ctttctgtat ctaacctgat tatcaatgag ttagctttat
    12421 cagctcgtga ttctgtgagc aaaagtagca accaccaggg atcgagacaa cttgttttat
    12481 caaaatattt aagttcgttt caagaaaagc ttccaacaat cgttgagtta actctttttg
    12541 atttaaatgg aaatgttgtt gcaagtagca taccagagtc tctaaatagt gttattcgcg
    12601 gtaatgagca gcacgatatg cttacgagta atatcaaaat taagccaccc tatcgaagtg
    12661 atcgatatga taccgctgtt ctagagcttt actccccagt tctctcctat gatgagaaag
    12721 tgattggctc aatattagca acactgaata tggaaaaatt catgagtagt ttagttgata
    12781 ataccgattt ttctttagga gaaatcactc tgctggggta cgcgggaaat acaattctga
    12841 gtagtaatca caagataaat tattcagctt cgctcgattc gaaaacttta aagcagttac
    12901 aagattacgg tggagaggcc ttggctttta agggacttac tcattcaaag gtaatcgggt
    12961 taatctcaaa atcgaaagag gcccctatta tcgttttagt agaaagagac tatgctgaag
    13021 ccattgctgc ctggatcgag ttacgtaatc tatttatgag tttagtaggt ttgctaattg
    13081 tgatcgtggg cggaatcgct ttttatattg gtcattctat cgtggcctcg ctgagctatt
    13141 tgatagaagg aacgaaacgt attttaaatg gtgatcttaa tgtacaaata aatgaaatta
    13201 aagcggatga aattggtcag ttgacagcga gctttaacaa gatgacggag aacttaagat
    13261 gtagtcaagc tgaaatattg gctgcacatg aagcaatgca aaagcaaaac cagcaattaa
    13321 aagttctttc aataacggat agcctgactg agttgtataa ccgcaacaag cttgacgcta
    13381 tacttattga tcagatagcg agatttgaac gaaatcaccg accattttcg gttgtaatga
    13441 tggatatcga tcatttcaaa acattaaatg ataattacgg tcatgtcatc ggcgatgaaa
    13501 ttttggtatc agtctcacaa gcaattagca aatcaatccg aagtgttgat tttgcggcgc
    13561 gatatggtgg tgatgaattt gttgtaatat tgactgagac ggacgcagca tctgctgtaa
    13621 ttaccgctga aaggattcgt gctcaagtag ctataaatgt aaattctcag atcaaagagg
    13681 ttagtctttc gatcacatta agtatgggga taatccaaag tgagatcgat gataaaaccc
    13741 ctaaaaatat attatcacgc gttgatggtg cgctttataa agcaaaaaat tcaggtagaa
    13801 atagaatata ttgcgcacat caccctagct gaaaagcaaa ttccttaatg tcatgctgcg
    13861 agtactctat ttttgcccga tcgtttggct agatacattg cttgatcagc acgcttgatc
    13921 gtggttttgg gatcttcatt ttcagcgagc tcagcgatac cgcagctaaa ggtaatcaat
    13981 atcttttcat tttgatgcag gaaaaagcag cgagttaaat ctctctggac acgctgcata
    14041 acggttacgc tatcgttaac ccctgttccc gttagtaaaa caacaaattc ctcaccacca
    14101 aaacgtgcca gggtatcttg gggcctaagg gatttgcgaa cgaccgctgc taaatggact
    14161 aacgcatcat cgcccgcttc atggccatgt gtatcgttta attttttaaa attatcgata
    14221 tcgagtaatg caatacacaa acttgagccg tcacgatact ttcgagaaac ttctcgctca
    14281 atggcttctt ccaaaccctt gcgattaaga gcgcccgtta atgcatcgtg gcgaatcagt
    14341 tcacttgcat gcaggagctc agattgcaat cgatcgactt ctttctcagc ggactcagca
    14401 cgggagcgga gttctaaaag ttcatcatgg gagcgttttg ctgaagattg aacttgtcga
    14461 gtttgctgga tgattctgtc aagaacatca gaaagttctg aaatatcttc tgcctggcta
    14521 atttcttcag cataaacacc tagactagcg tgataatcac cggtagtttg tgtaaactcc
    14581 gctaaccgat caacgaagga tgaaagcatg agcttcagtc gggactgatt atcaatcaga
    14641 ttttttttaa gaagcccttg tttgtatatg agttccttta tttgtcgttc gacattatca
    14701 attaacctta agtcgagcgg ctcagtaatc atttctctga caatggcgat ctgtccttgg
    14761 agccaagcgt catcaacgac taattcgctg atattatcga ttacgagttg aaataatttt
    14821 aagagcgaag ttctgagttc gccctggtct tctgcagcat attgcagttg atagttaaat
    14881 tgcttaagtt tatcggtaaa ctgtgaaagc tgcccagatt cagagataac tcgaacttcc
    14941 ctagcaagtg actcagcatc ctttgagatt tctggtgagg tagatagcaa tatacttaaa
    15001 cagtccccaa ataaacgccc taattggtct tgtaattccc tagggaggat cgatgtaatt
    15061 aatttgccag tttcattggg ttgtacgtta tctttttcaa ggtgatgatc attagaatga
    15121 tcgtttgaaa ttggattttg cttaatcaca ttaccgctta ataaatcgat cagtattgct
    15181 cccaaactct cccaatcacc ttttgatact gcagcttcaa atgactgaat gatctgttgt
    15241 tgatcagaat tattgcgagg taatttttct atgattattt ttaatgtttt ttctgggaag
    15301 aaatcaatta tctcaacccc agcaatttga taataaagcg aacggtaatt atcgggcgtt
    15361 ggtaatttgc gatttacagc gagctggcgt aatgcttctc gagcaatttc aatttctgtt
    15421 agtgttttcg aatttttcat gtgtaccaat aggaaaatct tgctatatgc ataaagtacg
    15481 aatgcagtat agcgataact ggtttcttaa atcataatag ctctaggttc gctttgttaa
    15541 taagttgata ccagcaaaac caatttgagc attgctgaga tccccgacga aattctcgtt
    15601 taattatttg agaatttttt gagcaacttt tcgctagttt agtgaa
//
LOCUS       DAOQKQ010000049        15121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_1802_length_15121_cov_6.147883, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000049 DAOQKQ010000000
VERSION     DAOQKQ010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 15121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15121
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1802_length_15121_cov_6.147883"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PKZ49_04730"
     CDS             complement(<1..247)
                     /locus_tag="PKZ49_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35902.1"
                     /translation="MYRNIAIDWMRGIVMVLMAIDHASAFFNQGRVANDTIVTHSVGS
                     IFVVEQFFTRWMTHLSAPTFVFLTGTAMALSISQRRAR"
     gene            complement(280..2010)
                     /locus_tag="PKZ49_04735"
     CDS             complement(280..2010)
                     /locus_tag="PKZ49_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35903.1"
                     /translation="MHSAFFRSTLQSLFLFPFVFLSGIGWITSSQLLAKEIETSQQND
                     PKPAYQLDTDEKGTVRIQNLGSHRFPVSTHNARAQLLIDQGLNLAYAFNHAEAYRAFQ
                     EAARLEPSLAIAYWGQALVLGPNINAVMEPKDEPRALALIQKAVSLLDSVSTKERALI
                     LALSKRYTGVANERVVNNHAYAAVMRLAYAQFPEDHDIAMLYVESMMNLRPWNYWMPD
                     GQPYEGTAEIVSITEDVLRKNPMHPGALHLYIHLIEPTNAPERAEAAADRLLTLMPDA
                     GHMVHMSSHIYQRVGRYADSMKSNQLAIAADEDYMAQCHAQGIYPMGYYPHNIHFLWF
                     AATLDGQSQPAIDAARKAASKINDETLVVLPAMAIFRVLPYWALTRFGHWQLMLEEPP
                     PPKASIFLTGSWHYARGLAFIATKQLQQAELALESLRSTLAVQDPNWDSPLFSPNTAR
                     SVLAIGPEVLAGEIAAAKGKLDEAIGHLERAVRLEDGLIYTEPAEWHFPPRLALGAIL
                     LEAGRPAEAETVYWEDLRRNRNNGWALYGLSQALRAQSKHGLARMIEDRFKLAWQRSD
                     LALHASRLIR"
     gene            complement(2108..2404)
                     /locus_tag="PKZ49_04740"
     CDS             complement(2108..2404)
                     /locus_tag="PKZ49_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35904.1"
                     /translation="MNSKLRNLAVAIVFSSFSSMSFAVNHGTSAEVKLYQLSEEVQHG
                     IITNLQGGEAEKIEKSGSDSSPIYEVQVKKSDKSGTEIDRFTIKVDSSGNLISN"
     gene            complement(2583..4640)
                     /locus_tag="PKZ49_04745"
     CDS             complement(2583..4640)
                     /locus_tag="PKZ49_04745"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HND35905.1"
                     /translation="MANGLTIAIFSAILALAFSGIWIRRIFSQSPGNERMQEIAAAVQ
                     EGASAYLKRQYMTIGLVGVILFVAISLALSWDTAVGFALGALLSGIAGFMGMTVSVRS
                     NVRTAEAARTGLNEALEIAFRGGAVTGMLVVGLGLLGVAGYCAMLYSGAEGDQAVSDV
                     IQPLVGFAFGGSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADNV
                     GDNVGDCAGMAADLFETYAVTIIATMLLGALLFSTNAANAVVYPLMLGAVSIVASIIG
                     CYYVKMRDDGKIMNALYRGLAVAGGIAFFAYLPVTVWFMGSMSLPLGDVVVGGGELVM
                     RLFICAAVGLVLTGLMVIITEYYTSTEYSPVKHIASASTTGHGTNIIAGLGVSMRATA
                     APVLAVCFSILVTYWLAGLYGIAIAATSMLSMTGIIVALDAYGPITDNAGGIAEMSGM
                     PESVRAITDPLDAVGNTTKAVTKGYAIGSAGLAALVLFADYTHGLEHAGKILTFDLSN
                     HMVIIGLFIGGMVPYLFGAMSMEAVGRAAGSVVVEVRRQFKEIPGIMEGTAKPDYSRA
                     VDMLTKAAIKEMIVPSLLPVLVPVLVGVILGPQALGGVLMGSIITGLFVAISMTTGGG
                     AWDNAKKYIEDDNFGGKGSEAHKAAVTGDTVGDPYKDTAGPAINPLIKIINIVALLII
                     PLL"
     gene            complement(4767..6026)
                     /locus_tag="PKZ49_04750"
     CDS             complement(4767..6026)
                     /locus_tag="PKZ49_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HND35906.1"
                     /translation="MAIKNAFYAQSGGVTAVINASAAGVLETARAHPDKIGKVFAGRN
                     GIIGALTEDLIDTSAESAEAIRALRHTPSGAFGSCRYKLKSLEQNRREYERLIEVFKA
                     HDIGYFFYNGGGDSADTCLKVSQLSGTLGYPLQAIHVPKTVDNDLPITDCCPGFGSVA
                     KYIAVSTREASFDVASMSKTSTKVFVLEVMGRHAGWIAAAGGLASSPDCEIPIVILFP
                     EIAFDKAKFLAKVDSCVKQYGYCSVVVSEGVKGEDGKFLSDQGLRDAFGHAQLGGVAP
                     VVANIIKDGLGLKYHWGVADYLQRAARHVASKSDVEQAYAMGKAAVEYAIAGHNSVMP
                     TIERLSDKPYNWKVGMAELSKVANVEKMMPADFITDDGFGITEKCRTYLSPLIEGEDY
                     PPYKNGLPNYVRLENKAVDKKLEEFKL"
     gene            complement(6089..6742)
                     /gene="adk"
                     /locus_tag="PKZ49_04755"
     CDS             complement(6089..6742)
                     /gene="adk"
                     /locus_tag="PKZ49_04755"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HND35907.1"
                     /translation="MRIILLGAPGAGKGTQANFIKEYFGIPQISTGDMLRNAVKAGTP
                     LGVMAKKIMDSGGLVSDEIIINLVKERIAEPDCTKGFLFDGFPRTIPQADALKVAQVA
                     IDYVVEIDVTDSEIIKRMSGRRVHPASGRTYHIVFNPPKVEGQDDISGEPLIQRDDDR
                     EETVMKRLEVYHAQTKPLIQYYSDWSARGDSGAPRYIKIAGTGDVEIIRQNIISQLS"
     gene            6948..7970
                     /gene="recA"
                     /locus_tag="PKZ49_04760"
     CDS             6948..7970
                     /gene="recA"
                     /locus_tag="PKZ49_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HND35908.1"
                     /translation="MDDNRNKALEAALSQIEKQFGKGSIMRLGTNDVANDIQVVSTGS
                     LGLDIALGVGGLPRGRIIEIYGPESSGKTTLTLQVIAEMQRAGGTAAFIDAEHALDPQ
                     YAKKIGVNIQELLISQPDNGEQALEIADMLVRSGSVDVVVIDSVAALTPRAEIEGEMG
                     DPQMGLQARLMSQALRKLTANIKRTNTMVIFINQIRMKIGVVFGNPETTTGGNALKFY
                     ASVRLDIRRTGSIKNGEEVIGNETRVKVVKNKVAPPFKQADFDILYGEGISRESEIIE
                     LGVLHKLIEKSGSWYSYKGEKIGQGKDNVRDFLKSHHDLAKELEQKIRTAVGLIPPGA
                     VSESTE"
     gene            7991..8437
                     /gene="recX"
                     /locus_tag="PKZ49_04765"
     CDS             7991..8437
                     /gene="recX"
                     /locus_tag="PKZ49_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HND35909.1"
                     /translation="MAQSNLKTLALQHLARREHSRLELEKKLSKYAQSTEALASTLDS
                     LEQQGSLSANRVVESIKQVRRAKYGSLRIHHELKTKGIAEELIDCAMIDLQQTELDAA
                     RQIWQKKFGVLPENWKTRGKQARFLAGRGFSPEIINQVFASLSEEI"
     gene            8483..11077
                     /gene="alaS"
                     /locus_tag="PKZ49_04770"
     CDS             8483..11077
                     /gene="alaS"
                     /locus_tag="PKZ49_04770"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HND35910.1"
                     /translation="MKSSEIRQQFLDFFESQGHTVVASSPLVPGNDPTLLFTNAGMVQ
                     FKDVFLGQDKRPYVRAATSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     RNAIQYAWEFLTRILQIPPHKLWITVYAEDDEAADIWLNEIGVDPKQFVRIATSDNFW
                     QMGDTGPCGPCSEIFYDHGPEVAGGPPGSTDADGDRYIEIWNLVFMQYNRDASGTLHP
                     LPKPSVDTGMGLERIAAVMQHVHSNYEIDLFQTLIKAAARVTQTTDLTNNSLKVIADH
                     IRACAFLVTDGVIPGNEGRGYVLRRIIRRAIRHGYRLEQKQPFFHLLVEDLAKVMGNA
                     YPELVAVKDQVSAVLKQEEERFAETLENGMQVLEAALQTQTKMLAGETVFRLYDTFGF
                     PIDLTADIARERGIGIDQAGFEQAMEDQRERARAAGKFTMQEGIQYHGSATEFHGYQY
                     LQQEARVLAVYQQGTPVEFIQPDEEAIIVLDRTPFYAESGGQVGDRGQILAGNGTFVV
                     ADTQKIQANVFGHKGVLSSGRLVVGDTVLAQVNPVTRANTANNHSATHLLHAALRQVL
                     GKHVTQKGSLVDANRLRFDFSHHTAVQSNELCTIENLVNEQIRRNHAVETQQLKYDDA
                     IKKGAMALFGEKYGDEVRVVAMGEFSTELCGGTHVSQSGNIGFFKIVSESGVAAGIRR
                     IEAVTGQGAMAYVQQNEAHLIEVAETLKASPSEVTQKLSQILDHVKQTERELTALKTK
                     LALLQSTELGEAQIREVKGTKVLAFALEGANVKILRETLDRFKQQLKSCIVVLATTEA
                     NKVTLIAGVTDDLTAKVKAGELINFVAQQVGGKGGGRADMAQAGGTLPEKLPEALSGV
                     TSWVEQKL"
     gene            11121..11777
                     /locus_tag="PKZ49_04775"
     CDS             11121..11777
                     /locus_tag="PKZ49_04775"
                     /inference="COORDINATES: protein motif:HMM:NF012976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase"
                     /protein_id="HND35911.1"
                     /translation="MNILSRRKFLRLGLAGSAASITLAGNKAVSSLLPFTPVEAEGPF
                     YPVTPQEDKDEDLTRIKGHHTSAQGEPIYVTGQVMNHQGQPLANASVEIWQANAKGRY
                     RHPRDPNPAPIDNHFQGWAIVTTDVQGHFRFKTIKPGAYPASAQWIRPPHIHFKISKE
                     HYQELITQMYFPDEALNEADLLLQHKKPSERSLMIAKRGALLNAIEVFEFPIVLRTTI
                     "
     gene            11810..12763
                     /gene="trxB"
                     /locus_tag="PKZ49_04780"
     CDS             11810..12763
                     /gene="trxB"
                     /locus_tag="PKZ49_04780"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HND35912.1"
                     /translation="MTTKHSRLLILGSGPAGYTAAIYAARANLNPVIITGLAQGGQLM
                     TTTDVDNWPADVMGIQGPELMERFQKHAERFQTEIIFDHIHTAKLTEKPFTLIGDQGT
                     YTCDALIIATGASAKYLGMPSEEAYMGRGVSACATCDGFFYKGQDVAVIGGGNTAVEE
                     ALYLSNIARRVTVVHRRDKFRSEKILIDKLMNKATNGNVNLAFNHVLDEVIGDASGVT
                     GIRIKQVQDGTTQHLEVKGVFIAIGHQPNTDLFQGQLEMRNGYLVTKSGNEGNATATS
                     ITGIFAAGDVQDQIYRQAVTSAGSGCMAALDAEKYLDHLGA"
     gene            12708..13352
                     /locus_tag="PKZ49_04785"
     CDS             12708..13352
                     /locus_tag="PKZ49_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HND35913.1"
                     /translation="MAVWLHWTQKSISITSVPKDKTPRPPDNPISDDEMLAFQEAMRG
                     VTPLAAPNCVLPQPQQPEFVRSPANELPSTKAEWSDHIALSLAAGEEWSYLRPGLSRQ
                     ILRKLRRGQWKVSAELDMHGMTKDQARQALATFLLECKQFNIRCVCIIHGRGLSSKNN
                     EPILKSRVGNWLAQCEDVLAFCQAPPTLGGSGAVIVLLKSQRSDQPNATTKTWD"
     gene            complement(13336..13707)
                     /locus_tag="PKZ49_04790"
     CDS             complement(13336..13707)
                     /locus_tag="PKZ49_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HND35914.1"
                     /translation="MADFTEAQLSQLKSAMQKRYLALQEEIHNELEHVSDSRYIDLTS
                     GFSGIPDDIDTARVDRQVNEMRQLEEALKQLNDLNFGDCIDCTSEIGFERLLAYPAAQ
                     RCIKCQKHHDRMYRHEASPTF"
     gene            complement(13785..15032)
                     /locus_tag="PKZ49_04795"
     CDS             complement(13785..15032)
                     /locus_tag="PKZ49_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HND35915.1"
                     /translation="MDKQPSSINQDRVHRIKLPQAVIVLGLVSFFNDVASDMVIPLIP
                     ILLASVLASGPIALGLIEGIADALASFLKLWSGRHTDVMGGRRKALAVVGYSLSNFTR
                     PLLGIATSWLSVLILRSIDRVGKGLRSAPRDALIADAAPAEIRGYAFGFHRALDNSGA
                     VAGSLIAAAILAWSGLNLAEVILWSALPGAIAVVLFIVGVKETQQNTQRGDTNLVQIP
                     TTNLPPLRWSSLSSSTQRYLIVLVLFTFARASETFIILLGYQLGIEVVELLIIWAAIN
                     LSKALTSTLGGQWADRYGRGNVMLISWVAFAIAFLLLSFATNNIELWAISVFYGLFAG
                     LGEGAERALIVDYAHPKERGTAFGWYHLMTGLAAIPAGLLFGSLWQGYSAALAFVVAS
                     GLAVIASILLRLWAWPNYGRIIR"
BASE COUNT         4399 a         3626 c         3204 g         3892 t
ORIGIN      
        1 cccttgcgcg tcgttgtgaa atactcagcg ccatcgcggt acctgttaaa aaaacaaaag
       61 taggcgcact taaatgcgtc atccagcgtg taaaaaattg ctctaccaca aaaatagaac
      121 caacactatg cgtcacaatg gtatcgtttg caactcgccc ctgattaaag aaagcagatg
      181 cgtgatcaat cgccatcaaa accattacaa tcccgcgcat ccaatcgatt gctatattcc
      241 gatacataat ctcgagttaa ctctcaattc aaactaagac tagcgaatca gacgtgaggc
      301 gtgaagtgcc aaatctgaac gctgccaggc taatttgaat cggtcttcaa tcattctagc
      361 gagcccgtgc tttgactgtg cgcgtagtgc ttgacttaat ccatacagcg cccaaccatt
      421 gtttcgattg cggcgtaaat cctcccaata cactgtttct gcttcggcag gtcggcccgc
      481 ttctagcaga atagcaccca atgccaatcg aggcggaaag tgccattcag ccggttcagt
      541 ataaattaac ccatcttcca atcgcaccgc acgttcgaga tgtccaatcg cttcatctaa
      601 ttttcctttg gcagcagcga tttccccagc gagcacttca ggaccaatcg caagaactga
      661 acgcgcggta ttgggtgaaa atagagggct atcccaattt ggatcttgca ctgctaatgt
      721 gctacgtaat gactccaatg ctagctcagc ttgctgaagt tgcttggttg caataaaagc
      781 caaaccacgt gcataatgcc agcttccggt caaaaaaatg ctcgccttgg gtggcggtgg
      841 ctcttctagc atcaattgcc agtgtccaaa acgagttaac gcccaatagg gtaatacacg
      901 aaatatcgcc atcgcaggaa gtacgaccaa agtttcatca ttaatcttgc tagctgcttt
      961 acgtgccgcg tcaatcgctg gttgactttg tccatcgagg gttgcagcga accacaaaaa
     1021 atgaatatta tgcgggtagt atcccatcgg atatattccc tgcgcatggc attgcgccat
     1081 ataatcttca tctgctgcaa tcgctaactg attactcttc atcgaatctg cgtagcgacc
     1141 gacccgctga taaatatgag aggacatatg aaccatatga cctgcatccg gcatcaatgt
     1201 caataacctg tcagctgccg cttctgctcg ctcaggcgca ttcgtcggtt ctatcaaatg
     1261 aatatataag tgcagagcac ccggatgcat gggatttttt cttaatacat cctctgtaat
     1321 agatacaatc tcggctgtac cctcataggg ttggccatca ggcatccaat aattccaggg
     1381 gcgaagattc atcatcgatt caacgtatag cattgcaata tcgtgatcct caggaaattg
     1441 cgcataagct aggcgcatca ctgctgcgta agcatgatta tttacaactc gctcatttgc
     1501 aacaccggtg taacgtttac ttaacgcaag tatcagtgcg cgttccttgg ttgaaactga
     1561 atcaagtaat gacaccgctt tttgaattaa tgctaaagcc cggggctcat cctttggctc
     1621 catgactgca ttgatgttgg gacctaacac gagtgcttgt ccccagtagg ctatagcaag
     1681 cgatggttct aatcgagccg cttcctggaa agcccgataa gcttcggcat gattgaatgc
     1741 ataggctaaa ttaagccctt gatcgatcaa taactgagct cgtgcattat gtgtactcac
     1801 tgggaaacga tgtgagccca ggttttgaat tcgtacagtt cccttttcgt cagtatctaa
     1861 ttgatatgct ggttttggat cgttttgctg ggaagtttcg atttcttttg ccaataattg
     1921 actggaagtt atccaaccta ttccacttaa aaaaacaaag ggaaataaaa acaaagactg
     1981 caacgtagat cggaagaaag cgctatgcat agcaattcct ttcaactgga attgattgat
     2041 tttccaggtc tgatcgaata atcaggcctg ggagcaacga attgattgat tgattgatta
     2101 ctaacgatta atttgagatt aaattgccgc tgctatcaac tttaatcgta aaccggtcaa
     2161 tctcagtacc tgatttgtct gatttcttga cttgcacttc ataaatggga gatgagtcag
     2221 atccactttt ctcaatcttt tctgcttccc caccttgtaa attcgtgatg atgccatgtt
     2281 gcacttcttc tgaaagctga tacagcttca cttcagcact cgtaccatga ttaactgcaa
     2341 aactcatgga cgaaaatgat gaaaatacga tagcaactgc aaggttacgt aattttgaat
     2401 tcatctttaa ttctcctatt taattaacgc gtaaatcaag aaacgaagga ataatgcttt
     2461 ctattaatcc tacactcatt tcttatcgta cgcattgcgt tgcagcaatg caacgaaaat
     2521 aaagtaaaaa gcccatcaag acacgttaat gggcttttta aattgctaaa atacaacaga
     2581 tactacaaca atggaataat cagtaaagca acgatattga taattttgat caacgggtta
     2641 atcgctggtc ctgctgtatc tttataagga tcaccaaccg tatcaccagt tacagctgct
     2701 ttatgcgctt cacttccttt gccgccaaaa ttatcatctt cgatatattt tttagcatta
     2761 tcccatgcgc caccgcccgt ggtcatagaa attgctacga ataaacctgt aataatagac
     2821 cccatcaata caccaccgag cgcttgagga cctaaaatta caccaacgag caccggaacg
     2881 agtactggta acagtgaagg tacaatcatt tctttgatcg ctgctttagt cagcatatcc
     2941 acagcacgtg aataatctgg cttggcagtt ccttccatga tacctggaat ttctttaaat
     3001 tgacggcgta cctcgactac gaccgatcct gctgcgcgac caaccgcttc catagacatc
     3061 gcaccaaata aataaggcac catcccgccg atgaagaggc cgataatgac catatgattg
     3121 gataaatcaa aagtcagtat ttttccagca tgctccagcc catgcgtata gtctgcaaat
     3181 agcaccaacg cagccaaacc cgctgagcca attgcataac ccttggtaac agccttcgtt
     3241 gtattaccca ctgcatccag cggatcggta atggcacgaa ctgattctgg cataccggac
     3301 atctctgcga taccgcctgc gttatcggta attggaccat aagcatcaag cgctacaata
     3361 ataccggtca tcgacaacat tgatgttgcg gcaatcgcaa ttccatataa ccctgcaagc
     3421 cagtaagtta ctaaaatact aaaacatact gctaatacag gagcagccgt tgcgcgcatt
     3481 gacacaccta aacccgcgat gatgttagtt ccatgtcctg tcgttgaagc gcttgcaata
     3541 tgttttactg gagaatattc agttgaagtg tagtactcgg ttatgataac catcaggcca
     3601 gttaagacca ggccgactgc tgcacaaata aataggcgca tcactaactc tccaccacca
     3661 accacaacat cacccagcgg caaagacata ctgcccatga accaaacggt tacaggtaaa
     3721 tatgcaaaaa acgcgatacc tcccgcaacc gctaagccac gatagagtgc attcatgatt
     3781 ttgccatcgt cacgcatttt gacgtaatag caaccaatga ttgatgcaac aatcgatact
     3841 gcgccaagca tcaggggata taccaccgca tttgctgcat tggtagaaaa tagcaatgcg
     3901 cccagcaaca tcgtcgcaat aatcgtcact gcgtaagttt caaacaaatc agcggccatc
     3961 cctgcgcagt cacccacatt gtcaccaacg ttatctgcaa taaccgcagg gttacgtgga
     4021 tcatcttcag gaataccggc ttccacttta ccgactaagt ctgccccaac gtctgcacct
     4081 ttagtaaaaa tccctccccc taaccgtgca aagatcgata tcaatgaacc accaaaggca
     4141 aaaccaacga gtggttgaat aacatcgctt acagcttgat caccctcagc cccgctataa
     4201 agcattgcac aatagccagc aactccgagc aatcccagac ctacgacgag cattcctgtt
     4261 acagcaccac ctcggaaagc tatctcaagt gcctcattca gaccagttct tgctgcttcc
     4321 gctgttctca cgtttgatcg aacagagaca gtcattccca taaagccagc aatccctgac
     4381 aaaagagcac ccaatgcaaa tccaactgcg gtatcccagg aaagcgccag gctaatcgct
     4441 acaaataaga taactccgac caagcctatc gtcatatatt gacgcttcag ataggccgat
     4501 gcaccttctt gcactgcggc agcaatttct tgcatacgtt catttccagg agattggctg
     4561 aaaatacgcc gaatccagat accactgaat gccagggcca aaatcgcgct gaatattgcg
     4621 atcgttaaac catttgccat ataaaactcc aaataaagcc agaccacctg aaaaacatgc
     4681 gaaattcggt ctggtaattt cgcatcaact ctctaaaatc aaacaacaaa aagctatttt
     4741 attacattcc ttaaataagg aaaaaattaa agcttgaatt cttccagttt tttatcaact
     4801 gctttattct caagccgcac gtagtttggt agtccgtttt tatacggagg gtaatcctct
     4861 ccttcgatca atggagaaag ataagttcta catttttccg tgatgccaaa gccgtcatcg
     4921 gtaataaaat ctgctggcat cattttttca acattggcta ctttcgataa ttcagccatt
     4981 cctactttcc agttataagg tttatcagaa aggcgctcaa tggtcggcat cactgaatta
     5041 tgtccagcaa ttgcatattc tacggctgct tttcccatcg cataggcttg ttcaacatcg
     5101 gatttggatg caacatggcg cgccgcacgt tgcagatagt cagccacccc ccagtgatat
     5161 ttcaaaccaa gcccatcttt gataatgtta gctaccacag gtgcaacgcc acctaattgt
     5221 gcatgcccga aagcatcacg gagcccttga tctgacaaga atttaccatc ttctcctttg
     5281 accccttcag atacgacaac tgagcaataa ccatattgct taacacagct atcgactttt
     5341 gcgagaaact ttgctttatc aaaagcaatt tcggggaata aaattacgat tggaatctca
     5401 cagtctggac tcgatgctaa tccgccagca gcagcaatcc aacctgcatg tcgacccatt
     5461 acttccagca caaacacttt agtagaagtc ttcgacatac tcgccacatc gaagctcgct
     5521 tctcgggtcg atacggcaat atatttcgca accgaaccaa acccagggca gcaatccgtt
     5581 atcggtaaat cattgtcaac ggtcttgggc acatggatcg cttgaagggg ataaccgagt
     5641 gtacccgaca attgtgaaac tttaagacac gtatccgcag aatctcctcc accattgtag
     5701 aagaaataac cgatatcatg cgccttgaag acttcgatga gccgctcata ctcgcgtcga
     5761 ttttgttcca ggcttttcaa tttgtagcga caagagccaa atgcgcccga tggtgtatgt
     5821 cgtagtgctc tgatcgcttc agcagattct gcactcgtat caattaaatc ttctgtcaac
     5881 gccccaataa ttccatttct tcctgcaaat actttaccaa ttttatctgg atgcgcgcgc
     5941 gctgtctcta aaacacctgc ggctgaggca ttaattactg cggtcacacc tcctgattgt
     6001 gcataaaatg cattttttat tgccatagca agacctctct tcgttttgtt atttattctt
     6061 taatcatgaa tctatttagc ttttactctt agctaagctg tgaaatgata ttttgtcgaa
     6121 ttatctcaac atctccagta ccagctatct tgatatagcg gggcgcaccc gagtccccac
     6181 gagctgacca gtccgaatag tactgaatga gtggtttcgt ttgtgcgtgg taaacttcta
     6241 ggcgcttcat aaccgtttct tctctatcat catcacgctg aatcaggggt tcaccactga
     6301 tgtcatcctg tccttccact ttgggtggat tgaatacgat gtgataagta cgaccagaag
     6361 caggatgaac tcgccgtccg gacattcgct taataatttc agaatcggtt acatcgattt
     6421 cgacaacata atcaatagca acctgcgcta ccttcaacgc atctgcttga gggatcgtgc
     6481 gcggaaaacc gtcaaataaa aaccctttcg tgcaatcggg ttcagcaatc cgttctttga
     6541 caagattgat aataatttca tcggacacga gaccgcctga atccatgatc ttcttggcca
     6601 tgacccccaa tggagtacct gccttaaccg cattacgcag catgtccccc gtagaaattt
     6661 gcggaatacc aaaatattct ttgataaagt ttgcttgcgt gcccttaccc gcacctgggg
     6721 cacctaataa aatgatacga atgatcttct cccttttttc tactcttttt taccggtatt
     6781 tgcaaaaata attgttattt tttaatttga agtttaatta tatctcaagc agaccatcct
     6841 cttgaagtat ttgctaattt ttgcatttat ggtattgaaa taaagtagat atctttcttc
     6901 ctggaatgaa tatgagataa tccagagaac aaacattagg aaaaaagatg gacgacaata
     6961 gaaacaaggc gttagaagcg gccctctctc aaatcgaaaa acaatttggg aaaggttcga
     7021 tcatgcgact gggcaccaat gatgtagcca atgatattca agtcgtatcg actggatcgc
     7081 tcggtttgga tattgcccta ggagttggcg gtttaccgcg ggggcgtatt atagaaattt
     7141 atgggcctga atcttcgggc aaaaccacac tgacgttaca agtcattgct gaaatgcaac
     7201 gcgctggcgg aacagcagcc tttatcgatg cagaacatgc actcgatcct caatatgcta
     7261 aaaaaattgg ggttaatatt caggaattgc tgatttcgca accggataat ggcgaacaag
     7321 cattagaaat cgctgacatg ttggttcgct cagggtctgt cgatgttgtt gtgatcgatt
     7381 cggttgctgc ccttaccccg cgtgctgaaa tcgaaggtga aatgggagat ccccaaatgg
     7441 gactacaagc taggctcatg tcccaagcgc tgcgaaaact gactgccaat atcaaacgca
     7501 cgaataccat ggtgatattt atcaaccaaa tccgtatgaa aattggtgtg gtatttggta
     7561 atccagaaac gacaaccgga ggaaatgcgc tcaaattcta cgcatccgtt cgcctggata
     7621 tccgtcgcac tggttcgatc aaaaatgggg aagaagtcat tggtaacgaa acacgcgtta
     7681 aagtagttaa aaataaggta gcccccccct ttaaacaagc tgacttcgat atcttgtatg
     7741 gtgagggaat ctcacgtgaa agcgaaatca tcgagctggg cgtgttacat aaactcatcg
     7801 aaaaatcagg ctcatggtac tcctataaag gagaaaaaat tggccaaggg aaagacaatg
     7861 tacgagattt cttgaaatca catcatgatt tagccaaaga actcgagcaa aaaattcgta
     7921 cggcagttgg tttaataccc cctggcgcgg tgagtgaatc aactgaataa taaacacgac
     7981 gtgtgtgctt atggcgcaat caaatctcaa aacacttgcc ttacaacact tagcaaggag
     8041 agagcattca cggctagagc ttgaaaagaa actatcaaaa tacgcgcaaa gcactgaagc
     8101 gctagccagt acgctggatt cacttgaaca gcaaggatct ttgtcagcaa accgagttgt
     8161 cgagtccatt aagcaggtgc gcagagccaa atatggatcg ttgcgtatcc accatgaatt
     8221 aaaaacgaaa ggcattgcag aagaactgat cgattgcgca atgatcgatc tgcaacaaac
     8281 cgaattagac gctgcccgcc aaatttggca gaaaaaattt ggtgtacttc cagaaaattg
     8341 gaaaacgcgc ggtaaacaag ctcgttttct agcaggtcgt gggttttcac ctgaaattat
     8401 caatcaagtt tttgcgagtc tttctgaaga aatttaatac gattctccct attactatta
     8461 ttgcttgaaa agcgaaattg acatgaaaag tagtgaaatt agacaacaat ttctagattt
     8521 ttttgaatca caaggccata ccgttgtcgc atcgagtcca ctcgtgcccg gtaacgatcc
     8581 aaccttgctt ttcacgaatg cggggatggt tcagtttaaa gatgtcttcc tcggccaaga
     8641 caagcgacct tatgtacgcg cagcaacgtc acaacgctgt gtacgcgcag gtggtaagca
     8701 taatgatctc gaaaatgtag gatacacggc acggcatcat actttttttg aaatgctggg
     8761 taattttagc tttggagatt atttcaagcg caacgccatt cagtatgctt gggaattctt
     8821 gacgcgcata ttacagattc caccccataa actttggata actgtctatg ctgaagacga
     8881 tgaagcagcc gacatctggc tcaatgaaat cggcgtagat cccaaacaat ttgtacgcat
     8941 tgcgacctca gataatttct ggcaaatggg tgataccggg ccctgcggtc cttgttctga
     9001 aatcttttat gatcatggtc cagaagttgc tgggggtccc cctggatcga ctgatgcaga
     9061 tggagaccgg tatattgaaa tctggaatct ggtattcatg caatataacc gagatgcgag
     9121 cggtacattg caccctttgc caaaaccttc ggtcgatacg ggaatgggac tggaacgaat
     9181 tgctgcagtc atgcagcatg tgcatagtaa ttatgaaatt gatttgtttc aaactttgat
     9241 caaagcagcc gcacgggtaa cacaaacaac tgatttaacg aataattcac tcaaagttat
     9301 cgcagaccat atccgcgctt gtgcatttct cgttacagat ggcgtcattc ctgggaatga
     9361 agggcggggt tatgttctgc gccgaatcat tcggcgcgct atccgacatg gctatcgact
     9421 ggagcaaaaa caaccttttt ttcatttact ggtcgaggat ctcgcaaagg taatgggtaa
     9481 tgcctatcca gagctcgttg ccgtgaaaga ccaagtttcc gcagtactca agcaagaaga
     9541 agaacgcttc gcggaaacac tcgagaatgg catgcaagtc cttgaagccg cattacaaac
     9601 ccaaacaaaa atgttagcag gcgaaacagt tttccgcttg tacgatacgt ttggattccc
     9661 tatcgatctt accgcagata ttgcccgtga gcgaggcatt ggtattgacc aggctggatt
     9721 tgagcaagcc atggaagatc aacgcgaacg tgcgcgtgca gccggaaaat tcacgatgca
     9781 agaagggatc caataccatg gcagcgcgac tgaatttcat ggctatcagt acttacagca
     9841 ggaagctcgc gttcttgcag tttatcagca aggaacaccc gtcgaattca ttcagccgga
     9901 tgaagaagcg atcattgtgt tagatcggac ccctttttat gccgaatcag gtgggcaagt
     9961 gggtgatcgg ggccaaatac tcgctggaaa tggtacgttt gttgtagctg atacccaaaa
    10021 aattcaggcg aatgtgtttg ggcataaagg ggtattgagc agtggacggc tcgtggtcgg
    10081 agatacggta ctggcccagg tcaatcccgt tacacgtgcc aataccgcta acaatcattc
    10141 tgcaacccat ttattacatg ctgcactgcg ccaagttctg ggtaaacatg taacccagaa
    10201 aggatcactg gttgatgcaa atcgactccg tttcgatttt tctcatcata ccgctgtaca
    10261 gtctaatgaa ttatgcacta tcgaaaattt ggtcaatgaa cagattcgac gtaatcatgc
    10321 agttgaaaca caacaactga aatacgatga tgctatcaaa aaaggtgcga tggccttgtt
    10381 cggagagaag tatggcgatg aagttcgtgt cgtcgccatg ggtgagtttt cgactgaatt
    10441 atgtgggggt acccatgtat cgcaaagcgg gaatatcggt tttttcaaaa ttgttagtga
    10501 atctggggtt gctgcaggca tccgtcgtat cgaggccgtt acaggccaag gcgctatggc
    10561 atatgtgcaa caaaatgaag cgcacttaat tgaagttgca gaaacattaa aagcttcacc
    10621 ttcggaagta acgcaaaaat tatcgcagat tctcgatcac gttaaacaaa ctgaacgaga
    10681 actgacggca ctcaaaacta aattggcctt gctacaaagc accgaattgg gtgaagcaca
    10741 aattcgtgaa gtaaaaggaa caaaggtact cgcatttgcg cttgaaggtg caaatgtcaa
    10801 aattctgcgt gagaccttgg atcgatttaa gcagcaattg aaatcgtgta tcgttgttct
    10861 agccacgacc gaagccaata aagttacctt aattgcgggt gttaccgatg atctcactgc
    10921 aaaagtcaaa gccggtgaat tgatcaattt tgttgcccaa caagtcgggg ggaaaggcgg
    10981 cgggagagcc gatatggcgc aagcaggcgg tacattacca gaaaaactcc cggaagcact
    11041 aagcggtgta acaagctggg ttgaacagaa attatagtca ccagcattgt gaacaaatgt
    11101 aaacgatagg aaataagcgc atgaacattc tatcgaggcg aaaattccta agactcgggt
    11161 tagctggtag cgcagcaagt ataacacttg ctggaaataa ggctgttagt tcattattac
    11221 cttttacacc ggtagaagcc gagggcccct tttatccggt tacacctcaa gaagacaaag
    11281 atgaagacct cactcggata aaaggccatc acacctcagc acagggtgaa ccgatttatg
    11341 taactggaca ggtcatgaat caccaggggc aaccgcttgc gaatgcgagc gttgaaatct
    11401 ggcaagcgaa tgcaaaaggc cgttaccgtc atcctcgcga tcctaatcca gcaccgatcg
    11461 ataaccattt tcagggctgg gctatcgtca cgactgatgt gcaaggtcat tttcgattca
    11521 aaaccatcaa accgggtgca tacccggcat cagcgcagtg gatccgaccg cctcatattc
    11581 acttcaaaat ttcgaaggaa cattatcagg aattgatcac acaaatgtat tttcctgatg
    11641 aagcgcttaa tgaagctgat ttactgttgc aacacaaaaa accttctgaa cgctcattga
    11701 tgattgccaa aagaggcgca ctgcttaacg caatcgaagt ttttgagttc ccgattgtat
    11761 taagaactac aatctaattt tttaattatt aaatcgactc atttaaatca tgacgactaa
    11821 acattcccga ctacttattc tcggttctgg tcctgccgga tacaccgctg cgatctacgc
    11881 tgcacgcgcc aatctcaacc ctgtgatcat tacgggcctg gcccaaggcg gtcaactgat
    11941 gacgactacc gatgtcgata actggcctgc tgatgtgatg ggtatacaag gtcctgaact
    12001 catggagcgt tttcaaaagc atgcagagcg cttccagaca gaaatcattt tcgatcatat
    12061 ccataccgct aaattgaccg agaaaccgtt tacgctgatc ggtgatcaag gtacctatac
    12121 ttgtgatgct ttgatcattg ccacaggtgc ttccgcaaaa tatctaggca tgccttcgga
    12181 agaagcttat atgggccgcg gcgtttctgc ttgcgcaacc tgtgatggct ttttctataa
    12241 ggggcaagat gtagcggtca tcggtggtgg caataccgct gttgaagaag cgttatatct
    12301 ttccaatatt gcgcgacgtg ttacagttgt tcaccggcgt gataaattcc gatccgaaaa
    12361 aatactgatc gataaactca tgaataaagc caccaatggt aatgtgaact tagcgttcaa
    12421 tcatgtatta gatgaagtca tcggtgatgc ttccggtgtt acgggcattc gcatcaaaca
    12481 agttcaggac ggaacaactc agcatttaga agtcaaaggc gtattcattg ccatcggcca
    12541 ccaacccaat accgatctct ttcaaggtca attagaaatg agaaatggct atttagtcac
    12601 caaaagcggc aatgagggta acgccactgc aaccagcatt acgggcatat ttgcagcagg
    12661 cgatgtgcaa gatcaaatct accgccaggc agtaacgagt gcgggtagtg gctgtatggc
    12721 tgcattggac gcagaaaagt atctcgatca cctcggtgcc taaagacaag acaccccgcc
    12781 ctcctgataa tccaatcagt gacgatgaaa tgcttgcgtt tcaggaggcg atgcggggag
    12841 tcactccact tgcagcgccg aattgcgtgc taccccaacc tcagcaacct gaatttgtcc
    12901 gcagccctgc taatgagtta ccttcaacca aagcagagtg gtcagatcat attgcgctaa
    12961 gcctcgctgc aggtgaggaa tggtcttatc tgcgtcccgg cttatcacgt caaattttac
    13021 gtaaattacg ccgtgggcaa tggaaagtca gcgctgaact tgatatgcat ggtatgacaa
    13081 aagatcaagc aagacaggcc ttggctacat ttttgctcga atgtaagcag tttaatattc
    13141 gatgtgtgtg tatcattcac ggtagagggc tcagttcaaa aaataatgag cccatactga
    13201 aatcccgagt tggtaattgg ctggcacagt gtgaggatgt tctcgcattc tgccaggctc
    13261 ctcctacttt gggtggtagt ggggccgtca ttgttttgct taaatcgcag cgatccgatc
    13321 aacccaatgc tactactaaa acgtgggact agcctcgtgc cgatacatgc ggtcgtgatg
    13381 cttctggcac ttaatgcaac gctgtgctgc aggataggct aataagcgtt caaaaccaat
    13441 ttcactggta caatcaatgc aatctccaaa attaaggtcg ttgagctgct tcaaggcttc
    13501 ctcaagctga cgcatctcat tgacttgccg atcgacacgc gccgtatcaa tatcatcggg
    13561 tattccagag aaaccactgg ttagatctat ataacgagaa tcggacacat gctcaagttc
    13621 attatgaatc tcttcttgta aagccaaata acgcttttgc atcgcgctct ttaattgact
    13681 taattgtgct tcggtaaaat cagccatgcc attctttctc ctacccgcat cacggttttt
    13741 tttaggttta gtcaacgaag ggaagaatct tcctgccttc tcacttatct tattatccta
    13801 ccatagttag gccaggccca taatcgcagc aaaatcgatg caatcactgc caaaccgctt
    13861 gccacaacaa aagccagcgc tgcactataa ccttgccata aggagccgaa cagcaagcca
    13921 gccggaatag cagccaatcc agtcattaga tggtaccaac caaatgcagt tccacgttct
    13981 ttcggatgcg cataatcaac gatcaaggca cgctcagcac cctctcccag acctgcaaat
    14041 aacccataaa acacactaat cgcccataac tcgatattat tagtagcaaa gctcaacagt
    14101 aaaaatgcaa ttgcaaacgc gacccaactg attaacatca cattgccgcg tccatatcga
    14161 tcagcccatt gccctcccag cgttgatgtc aatgccttgg acagattaat cgcagcccaa
    14221 atgatcaaca actcgacaac ctcaataccc agttgatatc ctaacaagat gatgaaggtt
    14281 tctgaggcgc gtgcaaatgt aaatagtact aaaactatca aataacgctg cgttgatgat
    14341 gataatgatg accaacgcag cggcggcaaa ttcgtagtcg ggatctgaac taaatttgta
    14401 tcgccacgct gagtattctg ctgcgtttct ttgacgccta cgataaataa cactacggca
    14461 atcgcgcctg gcagcgctga ccataagatt acctctgcca gattcaatcc cgaccaggcc
    14521 aatatggctg ccgcaatgag actacccgca actgccccac tattatcgag cgcgcgatgg
    14581 aaaccaaatg cgtagccacg aatctcagca ggtgctgcat cagcgatcaa agcatcgcgg
    14641 ggtgcactgc gcaatccttt cccaacccga tcgatactgc ggagtatcaa aaccgataac
    14701 cacgaagtag caattcccag caaaggacgg gtgaaattcg atagggagta accaactacg
    14761 gcaagtgcct tacgccgacc acccatcaca tcagtatgcc ttcccgacca aagcttgaga
    14821 aaactcgcca gtgcatcggc aataccttcg atcaaaccta atgcgatagg ccctgaagct
    14881 agaaccgagg ctaataaaat aggaatgagt ggaatcacca tatcagatgc cacatcattg
    14941 aagaaactca ctaacccgag cacaatgacg gcttgtggca attttatacg gtgaacacga
    15001 tcttggttaa tcgatgacgg ttgtttatcc atctcctcat aatagcgtaa aatgagagtt
    15061 cttttacgcc ctggtagctc agtggataga gcaaccccct cctaaggggt aggtcgcacg
    15121 t
//
LOCUS       DAOQKQ010000050        15028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_1819_length_15028_cov_6.083016, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000050 DAOQKQ010000000
VERSION     DAOQKQ010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 15028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15028
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1819_length_15028_cov_6.083016"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..2999)
                     /gene="prsT"
                     /locus_tag="PKZ49_04800"
     CDS             complement(195..2999)
                     /gene="prsT"
                     /locus_tag="PKZ49_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM system TPR-repeat protein PrsT"
                     /protein_id="HND35916.1"
                     /translation="MAITNYYKGTTKKKFTTVLVLILLILTTITACGKAKDADALISE
                     AKQYHEKGDDKAAIIQLKNALQQKPDDREARHLLGAIYNRTGDVRSAEKELSRALSLG
                     MDPARVLPDLGQALLSLGEFQQVLDKTETLPEDVDFAEILTLRGNALLGLGRFQEAKE
                     LFDQALQDKPDFPDAFIGLARHALSQKDVETAMRFLEEVTTKFPDNTNALIFKGDLLR
                     AQGKNDLALDVYGQAIKARPDQVTGYVNRASVLIGLGKFDSAQQDLNTASKIAPGNLL
                     IAFNQALLDFTQGKHAVALDALQKILSSAPNHMPSVLIAAASHFALGSVLQAEQLVNQ
                     YLKVIPNNLYAIKLKASILLKNHAAKQAIDLLTPALKSAQQDPQLLALAGESYMQSKD
                     FDKATEFFEKANALLPESAALHTALALSKLGQGNRESAIAELEAAANLDEQSPRAGVL
                     LVMTHLQTKDFSKALKAAEELEKEQVDSPLVQNLKGSIYVGMQDLAKARASFEKALAL
                     QQDYFPAISNLARLDVQENNLERAKNRFEEVLKRDKKHIQAMHALAGIALTQNNKNEA
                     ITWFERASNENPNELQPALQLGALYVRLQEGNKALSLAKKLQGSYPDNLSVVELLAQA
                     QLAVNDRVAALENYQKLAARLPDSAAAQFQLANVQALLQNQKAAAEAVKKAIALKPDY
                     LEANLLQARLEIQNNKPDEAMKIAKAIQKQHAKLPVGFELEGDLLMSQKKAAQAIGAY
                     EKAFSLNQSSQLMIKQYAALSQGGKGKQANERITKWLEQHPTDASTRLFLGGVHLEKK
                     QFDAAIKQFQMASEQNPDNPIILNNLAWTYQQIKDPSALDYAEKAYKNAPESPAILDT
                     LGWILVEKGEIARAVSLLQKAVSLAPEAAEIRYHLAVSLQKSGDKGAARQELEHLLAS
                     GKAFSKIDDAKKLLKDLEKD"
     gene            3286..4566
                     /locus_tag="PKZ49_04805"
     CDS             3286..4566
                     /locus_tag="PKZ49_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HND35917.1"
                     /translation="MDTVAVVGLGYVGLPLAVEFGKKRNTIGFDLSTSKIESYKRYID
                     PTDEVSTADLKAATQLTVTADPTELSKADYIIVAVPTPVDQAHQPDFSALIGASEIVG
                     RNMKRGAIVIYESTVYPGATEEVCIPAIEKYSSLQWKKDFFVGYSPERINPGDKQHTL
                     TTITKVVSGDSPETSEKVAHLYESVITAGVYRASSIKVAEAAKVIENTQRDLNIALMN
                     ELAIIFDKLGIDTLEILQAAGTKWNFLPFRPGLVGGHCIGVDPYYLTHKAEMVGYIPQ
                     VILAGRRINDNMAKFIAEQTIKQIIKAGFNVKGAKVIVLGLTFKENCPDLRNSKVADV
                     ISELKDYGVDVYVHDPIAEAKEAEHEYGVTLQSWENLPKAHAIVAAVSHRDFIQKPLA
                     EICLKLVDKGCFIDVKSQFNESELCKAGLSVWRL"
     gene            4582..5610
                     /locus_tag="PKZ49_04810"
     CDS             4582..5610
                     /locus_tag="PKZ49_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HND35918.1"
                     /translation="MTRYQIIQQTLSTRPYRWLVTGVAGFIGSNLLEALLKLNQKVVG
                     LDNFSTGYQHNLDQVKAAVSPTIWANFTFHHGDIRSLQDCQKACESIDFVLHHAALGS
                     VPRSIEDPIQTNESNISGFINMLVAARDANVKRFVYAASSSTYGDHPDLPKIESVIGR
                     PLSPYAVTKYVNELYADVFARCYGVQSIGLRYFNIFGPRQDPNGAYAAVIPQWIAALI
                     QNKTLKINGDGETSRDFCFVENVIQANLLSALAEESAAINQVYNIALNERTSLNQLYS
                     MMRKLLINKFPHLQNHQPIYVDFRQGDVRHSQADISKAMNLLGYAPTHRIDKGLEEAM
                     DWYIENLS"
     gene            complement(5643..5921)
                     /locus_tag="PKZ49_04815"
     CDS             complement(5643..5921)
                     /locus_tag="PKZ49_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35919.1"
                     /translation="MTLKSTITEFNTYLGDKESILDKNYQRIAEQIKLLWGYDEFFVF
                     IEKLVLVEKERNRGGFPYEVILELNILQDIHRKKFSKRSVDQKIRFHS"
     gene            complement(6214..7359)
                     /gene="ftsZ"
                     /locus_tag="PKZ49_04820"
     CDS             complement(6214..7359)
                     /gene="ftsZ"
                     /locus_tag="PKZ49_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HND35920.1"
                     /translation="MYEIMNSEGQEAVIKVVGVGGCGGNAVDHMIQNATKGVEFICLN
                     TDAQALKGNRAPVLLQLGSGITRGLGAGANPDIGREAALEDRDRIAELIQGADMLFIT
                     AGMGGGTGTGAAPVVAQIAKEMGILTVAVVSKPFSFEGKRLKTAQAGMEALSQHVDSL
                     IVIPNDKLMMVLGNDISMLDAFKAANDVLYGAVAGIAEVINCPGLVNVDFADVKTVMS
                     EMGMAMMGSAIASGVDRARMAAEQAVASPLLEEITLSGARGVLVNITASSGMKMREVH
                     EVMNTIKELTAEDATVIVGTVIDEAMQDTLRVTLVATGLGSVTQSQSRPLTVIHTRTG
                     TDDRALSPFDEPAVMRTGRRNNATVAAMQQSGVDPLDIPAFLRRQAD"
     gene            complement(7434..8669)
                     /gene="ftsA"
                     /locus_tag="PKZ49_04825"
     CDS             complement(7434..8669)
                     /gene="ftsA"
                     /locus_tag="PKZ49_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HND35921.1"
                     /translation="MNKPKVERDLIVGLDIGTSKIVAIVAEIRPEGGFEIVGLGSYPS
                     RGLKKGVVVNIETTVSAIQRALEEVELMADCKISEVYTGIAGSHIRGFNSHGMVAIKD
                     KEVTAFDVEKVMETAKAVNIPADQQILHILNQEFIVDGQEDVREPIGMSGIRLEVKVH
                     IVTGAVSAAQNIIKCVHRCGLEVRDLILQPLASAIAVLSDDEKDLGVCLVDIGGGTTD
                     IAVFTNGAIRHTSVIPIAGDQITNDIAMALRTPTKDAEDIKCRYGCALRGLADVREMI
                     EVPDVGNRGARQLSKQTLAEVIEPRVEELYLLIQAELRRSGFEQLLSSGIVITGGTSS
                     LQGMVELGEEIFHMPVRLGLPKYSGGLEEVVKTPRYSTGIGLIMAGIDEQRHHHNHLK
                     GGSAKQVFARMKGWFQKNF"
     gene            complement(8688..9470)
                     /locus_tag="PKZ49_04830"
     CDS             complement(8688..9470)
                     /locus_tag="PKZ49_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HND35922.1"
                     /translation="MWNNHKALNLLANLILTGVVLSVAYFLVSSMVKLPLFDLKEIRI
                     ESVSNTDQSDKLALQHINRQQIQRVVGDAVIGNFFTVDLNRVKNAFQELPWVRVAKVQ
                     RNWPLGIKVILEEHSALAYWGDTALVNLQGEIFRAPSDKALPVFRGPTEESAALIAEQ
                     YKIMNRTLSPINQHVAEISLSPRYAWFIRLDNGTLLKLGRKKIKSRLERYISVYKNNI
                     VNLNQSIPLDYVDLRYPSGFAVRMDEVIPASTSPKPISRRKS"
     gene            complement(9460..10377)
                     /locus_tag="PKZ49_04835"
     CDS             complement(9460..10377)
                     /locus_tag="PKZ49_04835"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HND35923.1"
                     /translation="MNTVNSGKVAVLLGGRSAEREVSLRSGEAVLNALIRQGIDAHPF
                     DPSEQSLEELVRQGFDRAFIALHGRFGEDGSIQGALELLGIPYTGSGVLASALAMDKW
                     RTKLIWQAVGISTPRFLILDGQKAAEEVIDTLGLPLIIKPAREGSTIGLSKIKTPQAF
                     NEAYQLALNHDSIVLAEQFIEGKELTAAILGSKPLPLVRIEVSGDLYDYQAKYLSNDT
                     RYYCPSGLPDDLTEQIQRQALQAHTILGCYGWSRVDVILDHDHRPFFLEANTSPGMTD
                     HSLVPMAARAAGISFDQLVVQILALTHVE"
     gene            complement(10374..11369)
                     /gene="murB"
                     /locus_tag="PKZ49_04840"
     CDS             complement(10374..11369)
                     /gene="murB"
                     /locus_tag="PKZ49_04840"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HND35924.1"
                     /translation="MQLNAAVMDLFDFDNSIEQDIVDLGQLRGQLKINEPMGKHVSWR
                     AGGTARYFYIPQDRNDLSRFLSLLPEKIPLYMIGLGSNLLVRDGGFDGVIVGLHARLN
                     DLFISHMDGSGGSVFAGAGVSCAKLARFMGMSNLVGAEFLAGIPGTVGGALAMNAGCY
                     GGETWNIVERVQVIERSGNIIEKNIDDYQIGYRSVIRKSNDNKQGDEEWFAGAWFKLA
                     RGDGSVSKQKIKELLTARIAAQPLNYPNAGSVFRNPAGDYAARLIEFSNLKGLQIGGA
                     MISTKHANFIINTGNATAADIETIITEVRHRVREQTGVSLTQEVRIIGNTKVITQ"
     gene            complement(11413..12828)
                     /gene="murC"
                     /locus_tag="PKZ49_04845"
     CDS             complement(11413..12828)
                     /gene="murC"
                     /locus_tag="PKZ49_04845"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HND35925.1"
                     /translation="MKHKVKSVHFVGIGGAGMGGIAEVLINLGYKISGSDLMENMTTE
                     RLQKLGALIYYGHDKENVANVDVVVTSTAVKADNPEIIAARERHIPVVPRAMMLAELL
                     RLRQGIAIAGTHGKTTTTSLVASILAEAGYDPTFVIGGKLEAAGSHAKLGKGEFIVVE
                     ADESDASFLYLQPILTVVTNIDADHMSTYGHDFARLKQTFVEFIEHLPFYGMAVLCID
                     DQNIREILPNITKPITTYGLSPDAQVSASSVQHDNGRMCFRVNIGVNGTARQLDITLN
                     LPGLHNVQNALAAITIANEIGVPDAAIVRALATFGGVERRFQRYGDIKIPGKGSFTLI
                     DDYGHHPAEIAATISATRGAFPGRRLILAFQPHRYTRTRDVFEDFVKVLSTVDILLLT
                     EVYAAGEEPIVAADSKSLTRAIRVLGKLEPIYVEMPDELPSVIHGIVEDRDVVLIMGA
                     GSIGKVAAWVSKAAKKLVAIG"
     gene            complement(12818..13906)
                     /gene="murG"
                     /locus_tag="PKZ49_04850"
     CDS             complement(12818..13906)
                     /gene="murG"
                     /locus_tag="PKZ49_04850"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HND35926.1"
                     /translation="MKHTLLIMAGGTGGHVFPGIAVADYMRSLGWRVVWLGTQNGMEA
                     VLVPKYGYPVEYINFSGIRRKRIMDWLLLPGKLLLAFWQSIKILTRNQPSIVLGMGGY
                     PAFPGGLMAVLLNKPLVIHEQNAIAGLTNKILARVADKILLGFPGTFPQKDNRVVVTG
                     NPVRSKISELPLPEQRFSDRTGPIKILVIGGSLGASVLNKVIPQALSRIPEAKRPIVQ
                     HQAGRQHIDELRENYREQGVEGQLIDFIDDMAASYAECDLVICRAGALTITELAAVGV
                     ASILIPFPYAVDDHQTANAEYLSKPGAAVLLPQHKLNADSLADKIMELTRAQLLDMAK
                     IARNLANFNSAQQVAQVCIAMEKANNET"
     gene            complement(13941..>15028)
                     /gene="ftsW"
                     /locus_tag="PKZ49_04855"
     CDS             complement(13941..>15028)
                     /gene="ftsW"
                     /locus_tag="PKZ49_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HND35927.1"
                     /translation="ALISLGLIMIYSASIAIAESEYGEAGSFHYLTRHASYIIIGLIL
                     GSIAFQIPTRVWRTLVPYLFLGGVALLILVLIPGIGREVNGSQRWISLVIVNFQPSEL
                     MKLLMILYVSDYVVRKSLYLNDLRRGFLPLWLVIGVVGVLLLREPDYGAFVVITSIAV
                     VIMFLGGMSLKLFAGLASILVIGLVLLVLFEPYRMDRIIAFMDPWADPFGKGYQLSHA
                     LIAFGRGEWFGVGLGGSVEKLFYLPEAHTDFLLAVLAEELGFIGVMVVMMLFACLLIR
                     AYQIGRLAASLENYFSALVAQGIGIWLGLQALINMGVNMGVLPTKGLTLPLMSFGGSS
                     IVVSCIALAILLRIDSENRQRIRGMAV"
BASE COUNT         4376 a         3563 c         2699 g         4390 t
ORIGIN      
        1 ttattctact gaatcacttg ttaagtcatt gtgtagataa cttcgaaaat taaatttagc
       61 tttgttagca ggttaataaa ggggtgtatt tagaaatcta ttatcacgtt gaacctgact
      121 atttggctac agaaaaccta agatgatcaa ttcgttaagt tatccgtagc caccttatgc
      181 tcagatagga gtaattagtc tttttctaaa tcttttaata attttttagc atcgtcaatt
      241 ttggagaacg ctttccctga agctaataag tgctctaact cttggcgtgc tgcaccttta
      301 tcaccagatt tttgtagact tactgcaagg tgatagcgta tttctgctgc ctcaggcgca
      361 agtgaaactg ctttctgtaa aagagagact gcgcgtgcta tttctccttt ttctacgaga
      421 atccaaccta atgtatcgag aatcgcagga ctctcaggtg cgtttttata ggctttctcc
      481 gcatagtcta atgcagaagg atctttgatc tgctgataag tccaagcaag attattgagg
      541 ataattgggt tatcaggatt ttgttccgat gccatttgga attgtttaat cgccgcatca
      601 aactgtttct tttctaaatg aactccgcct aagaataaac gcgtggaagc atcagtagga
      661 tgttgttcaa gccacttcgt gatacgttca ttcgcttgtt ttccttttcc accttgactc
      721 aacgctgcat attgcttaat catcaattga ctcgattgat tgagcgaaaa agctttttca
      781 tatgcgccga ttgcttgcgc tgctttcttc tgactcatca acagatcgcc ttctaattca
      841 aagccaacag gtagttttgc atgttgtttc tggatcgcct tagcaatttt catcgcttca
      901 tcaggcttgt tattctgaat ttcaagccga gcttgtagta gatttgcttc cagataatcg
      961 ggcttcaacg caattgcttt tttgactgct tcagcagcag ctttctggtt ttgtagcaat
     1021 gcttgcacat tcgcaagttg aaattgtgca gctgctgaat ctggtaatct agctgctagt
     1081 ttttgatagt tttcaagcgc ggctacccta tcattaactg caagttgagc ttgcgcaagc
     1141 aattccacta cgctcaagtt atcggggtaa ctcccttgaa gtttttttgc aagactcaaa
     1201 gctttatttc cctcctgcaa ccgcacataa agcgctccta attgcaatgc gggctggagt
     1261 tcattggggt tttcgttact tgcacgctca aaccatgtga tcgcttcatt cttgttattt
     1321 tgcgtcagcg caattcctgc tagggcatgc atggcttgaa tatgtttttt atctcgcttt
     1381 agcacttctt caaaacgatt cttagcgcgc tctagattat tttcttgtac atccaatcta
     1441 gcaaggttag aaatagcagg aaaataatct tgttgcaatg caagtgcttt ctcaaagcta
     1501 gcgcgtgctt ttgctaaatc ttgcattccc acatagatac tccctttaag attctgaacc
     1561 aaaggactat ctacttgctc cttctcaagt tcttctgctg cttttaaagc tttgctgaaa
     1621 tctttcgtct gcaaatgagt cataacgagt aatacacctg cacgcggtga ttgctcatcc
     1681 aaattcgcag ccgcttctag ttcagcaatc gcactttcac gattaccttg gcctagctta
     1741 ctcaaagcaa gcgcagtatg aagtgcagca ctttctggaa gcaacgcatt cgctttttca
     1801 aaaaactcag ttgccttatc aaaatcttta ctttgcatat aactttcacc agccaatgct
     1861 aatagctgag gatcttgctg cgctgatttg agtgcaggtg tcaataagtc gatagcttgc
     1921 tttgctgcat gattctttag caagatagac gcttttagtt taattgcata aagattatta
     1981 ggaattactt ttagatattg attaacgagc tgttcagctt gcaatactga ccctaaggca
     2041 aagtgagaag ccgcagcaat cagcacgctc ggcatatggt tcggggctga acttaaaatc
     2101 ttttgtaagg catcaagtgc aactgcatgt tttccttggg taaaatcgag cagcgcttga
     2161 ttaaatgcga tcagtaaatt tcccggtgct attttgctag ctgtattcaa atcttgctga
     2221 gcggaatcaa attttcctaa accgattaaa accgaggcac ggttaacata cccagtaacc
     2281 tgatcaggcc tagctttgat tgcctgacca tatacgtcga gcgccaaatc atttttacct
     2341 tgtgcccgta acagatcgcc cttaaaaatt aaggcatttg tgttatccgg aaattttgtc
     2401 gtcacctctt ctagaaaacg catcgcagtt tcgacatctt tttgtgagag cgcatgtcga
     2461 gcaagcccaa taaatgcatc cggaaagtct ggcttgtctt gaagtgcttg atcaaataac
     2521 tctttagctt cttgaaatct gccaagtccc aataaagcat tacctctaag tgtcagaatc
     2581 tcagcaaaat caacatcttc tggcagtgtc tcagttttat ctaatacttg ctgaaactcg
     2641 cctaaactga ggagcgcctg ccccaaatca ggaagcactc tcgcaggatc catgcctaaa
     2701 ctaagtgcac gactcaattc tttctctgct gagcgaacat caccagtccg attgtaaata
     2761 gcgcctaaaa ggtggcgtgc ttcacgatca tctggctttt gctgtaatgc attcttgagc
     2821 tggataatag cggccttatc atctcctttt tcatgatatt gttttgcttc tgaaataagc
     2881 gcgtctgcat ctttggcttt accgcaagca gtaatcgttg taagtatcaa taaaattaaa
     2941 acaagaactg ttgtaaattt ttttttcgtg gtacctttat aataattagt tattgccatt
     3001 acttaatctc cctaatcaat aaattagaca gccaattcga tagaaaaacc cattctaatc
     3061 agttaattca attcccaaga tcagttttct cattagtcga gtctatacct tttttgaatt
     3121 tataccataa gtatatgata tagattttct aaaatagcgc atctttgttg tgttgttgca
     3181 cgagctaact tggatgattc catgtaatct atagcacaaa tgagaaacta atagggttaa
     3241 gaaacaaaag ctttttgaat aattagcgaa taaaaggaaa tgtttatgga tacagtagct
     3301 gttgtaggat tgggatacgt tggattaccg ttagccgttg agtttggaaa aaaacgtaat
     3361 accattggtt ttgatctttc aaccagtaaa attgaaagct ataagcgtta tatcgaccca
     3421 acagatgagg tatctaccgc tgatctaaaa gcagcaacac aattaactgt tacggccgat
     3481 ccaactgaat tatctaaagc agattatatt atcgtagctg ttccgacccc agttgatcaa
     3541 gctcatcaac ctgatttttc tgcactgatc ggtgcgagtg aaatagtagg tcgcaatatg
     3601 aagcgcggcg caatcgtcat ttatgaatca acggtttacc ctggtgcaac cgaagaagtt
     3661 tgcattcccg caatagaaaa atattctagc ttgcaatgga aaaaagattt ttttgtagga
     3721 tattcaccgg aacgcattaa tccaggggat aagcaacata cccttacaac cattaccaag
     3781 gttgtgtctg gagacagccc agaaacgtca gaaaaagtag cgcacctcta tgaatcagtg
     3841 ataactgccg gcgtctatcg agcttcaagt atcaaagttg ctgaagctgc gaaagttatc
     3901 gaaaataccc aacgtgattt aaatattgcg ctcatgaatg agctcgcaat catttttgac
     3961 aaattgggaa ttgatacatt agaaatcctc caggctgcag gaaccaaatg gaacttttta
     4021 cctttcagac ctggcctggt tggaggacat tgtattgggg tcgatccata ttatcttacc
     4081 cataaagccg agatggtcgg ctatattccg caagttattt tggcgggacg acgcattaac
     4141 gacaatatgg ctaaattcat cgcagagcaa acgatcaaac aaattattaa agctggcttt
     4201 aatgttaaag gcgctaaagt aatcgtgttg gggttaactt tcaaggaaaa ttgccctgat
     4261 ttacgaaatt ctaaagtagc agacgtcata tcagagctta aagattatgg agttgatgtc
     4321 tatgttcatg atcccatagc tgaggccaaa gaggctgagc acgaatatgg tgtaaccctg
     4381 caatcttggg aaaatctccc caaagcccat gcgatagtag ctgctgtctc gcatcgagat
     4441 ttcattcaga aaccattagc cgaaatttgc ttaaaacttg ttgataaagg atgctttatt
     4501 gacgtcaaat cacagtttaa cgaaagcgaa ttatgtaaag caggattaag tgtctggcgt
     4561 ctataactcg gtattcccaa tatgactcgg tatcaaatta ttcagcaaac actgagcacc
     4621 aggccctatc gctggttagt cactggcgta gctggtttta ttggatctaa tttactagaa
     4681 gcgttattaa agcttaacca aaaagtagtt ggtctcgata atttttcaac cggctaccag
     4741 cataacctcg atcaagtcaa agccgcggtt agtccaacaa tctgggctaa ttttacgttt
     4801 catcacggtg atattcgatc actgcaagac tgccaaaaag cttgcgagag tatcgatttt
     4861 gtattgcatc atgccgcatt aggctcagta ccacgttcaa ttgaagatcc tatccaaacc
     4921 aacgaaagca atatctcagg ttttataaac atgttagttg ctgctcgtga cgcaaacgtc
     4981 aaacggtttg tctatgccgc ttctagttct acctatggag atcatcccga tttgccaaaa
     5041 attgaatctg taatcgggcg cccgctgtct ccctatgctg tcaccaaata tgtcaatgaa
     5101 ctttacgctg atgtttttgc acgatgctat ggtgtccaat ccattggtct gcgctacttc
     5161 aatatatttg gtcctcgaca agatcctaat ggcgcctatg cagcggtaat tccacagtgg
     5221 attgctgcgt tgattcagaa caaaacactt aaaattaacg gggatggcga aacaagccgt
     5281 gatttctgct ttgtcgaaaa tgtaatccaa gccaaccttc tctcagcatt ggctgaagaa
     5341 tcggcagcca ttaatcaggt ctataacatt gcgttaaatg agcgcacaag cttgaatcag
     5401 ctttattcaa tgatgcgtaa gttattgatt aacaaatttc cccatctaca aaatcatcag
     5461 ccgatttatg ttgattttcg ccagggtgat gtacgacatt cgcaggcaga tatatcaaaa
     5521 gctatgaact tactgggcta tgcacccaca catcgaattg ataaaggttt ggaagaagcg
     5581 atggattggt atatcgaaaa tctctcctaa ttctgttgat caataagcta aacagatcaa
     5641 atttatgaat ggaatcttat cttctgatca acggatcgct tagaaaattt cttccgatgt
     5701 atatcctgta gaatgtttag ttctaatata acttcataag gaaatccccc tctattccgt
     5761 tctttctcaa caagcactaa tttctcaatg aacacaaaaa attcatcata tccccaaagt
     5821 aattttatct gctccgcgat tctctgataa ttcttatcaa gaattgattc tttatcacct
     5881 aaataagtat taaattcagt gatcgtactt tttagtgtca tttaccacta tctccttcca
     5941 ttttttctca attcgcttct aatctttatt taaaaatagg cctattgatg aaaaaaacaa
     6001 tgtatctttg aaaatatcgg caaatacaac tacaaagtta aaatgctgcg taatttagtt
     6061 gaataggact gagcatttac taatctaact taagattact taagttcata accttatcaa
     6121 ggtgttagta ctacccagtc ctaattaaat tacaataaca tgcgtctaac taaagaatct
     6181 ccccatgcaa aggtctaatt ctatcgttca actttagtcg gcttgtcgtc ttaagaaagc
     6241 aggaatatcc agtggatcga cccctgattg ctgcatagca gccacagtag cattatttct
     6301 tcttcccgtc cgcattacgg ccggttcatc aaacggtgaa agtgctctat catcagtacc
     6361 ggttcttgta tgaataaccg ttagaggtct tgactgtgat tgagtaacgc tacccaatcc
     6421 tgtcgcaacc agtgtcacgc gaagtgtatc ttgcatcgct tcatcgataa cggtacctac
     6481 gataacggta gcatcttctg cagtaagttc tttaatggta ttcatgactt catgaacttc
     6541 tcgcattttc ataccagagc ttgcagtaat atttaccagc acaccacgag cacctgataa
     6601 cgtaatttcc tctaataaag gactagcaac ggcttgttca gctgccatac gcgcacgatc
     6661 taccccactt gcaatcgcag agcccatcat agccattccc atttctgaca ttacggtttt
     6721 aacatcagca aaatccacat taacaagacc agggcaatta attacttcgg cgatacctgc
     6781 tacagcgcca taaagcacgt cattagccgc tttgaaggca tcaagcatac tgatatcgtt
     6841 accgagtacc atcatcagct tatcgttagg aatcacaatc aatgaatcaa catgctgcga
     6901 taatgcttcc atcccagctt gagctgtttt caagcgctta ccctcaaaag aaaaaggctt
     6961 actgacaaca gctacagtaa gtatacccat ctcttttgca atttgcgcta cgactggcgc
     7021 tgcaccagtg ccagtcccac ctcccattcc tgcggtaata aacaacatat cagcgccctg
     7081 gataagttct gctatgcggt cacggtcctc aagcgctgct tcgcgaccga tatcaggatt
     7141 tgcacctgcc cccagacctc ttgtaattcc tgaaccaagc tgcagcaaaa ctggtgcacg
     7201 atttccttta agggcttgag catctgtatt aaggcaaatg aactcaacac ctttagtcgc
     7261 attctgaatc atgtggtcaa ccgcattccc tccgcatcct ccaacaccaa ctactttaat
     7321 gaccgcttcc tgaccttcag aattcatgat ttcatacata acacctctcc tttgcatggt
     7381 aaatttcaaa taactacgct tctacacaac gcatacaacc taaaattttt tggtcagaaa
     7441 tttttctgaa accaaccttt cattcgtgca aatacttgct tagctgagcc acctttcaaa
     7501 tgattatggt gatgacgctg ctcatctata ccagccatga ttaaaccaat accagtcgaa
     7561 tagcgaggag ttttgaccac ttcctccaac cctcctgaat actttggtag gcctagccta
     7621 accggcatat gaaatatttc ctccccgagc tcaaccattc cctgcaatga gctcgtcccc
     7681 cccgtaatta caattcctga tgaaagtaat tgctcaaacc cacttctgcg cagctcagct
     7741 tggattaaca aataaagctc ttctactcgc ggttctatca cctcagcaag tgtttgctta
     7801 gaaagctggc gcgcacctcg atttcctaca tcaggaacct caatcatttc acgcacgtca
     7861 gcaagaccac ggagcgcaca cccataacga catttgatat cttcagcatc tttggtcggt
     7921 gtccgcagtg ccatcgctat atcattagta atttggtctc cggcgatcgg aataaccgaa
     7981 gtatgtcgaa ttgcaccatt ggtgaatacc gcgatatcag tagttccccc tccaatatca
     8041 acgagacaaa cccctaaatc tttttcatca tccgataata ctgctattgc cgaagcaagg
     8101 ggttgcaaaa taagatcacg aacttctaac ccacaacgat gtacacattt aataatattc
     8161 tgggcagctg acacagcccc cgtcacaata tgtaccttaa cctcaagccg tatcccactc
     8221 atcccaatgg gttctcgcac atcttcctgt ccatcaacaa taaattcttg atttagaata
     8281 tgaagtattt gttgatcagc tggaatattg actgctttgg ccgtttccat tactttctct
     8341 acatcgaaag cagtcacttc cttatctttg atcgcaacca tgccatgtga attaaaacca
     8401 cgaatatgac ttcccgcgat acccgtgtaa acttcactta tcttacaatc tgccatgagt
     8461 tccacttctt caagcgcacg ctgtatcgca ctgactgtcg tttcaatatt gaccacgaca
     8521 cccttcttaa gcccccgcga aggataactc cctaatccta caatttcaaa accgccctct
     8581 ggtctaattt cagcgacaat agcaacgatt ttagaggtac caatatcaag accaacaatc
     8641 aaatctctct caactttggg cttattcatt tatcaatccg ccttttttca tgattttctt
     8701 cgagatattg gttttggcga agtactagct ggaattactt cgtccattcg cactgcaaaa
     8761 ccacttggat aacgtagatc gacataatct aagggaatag attgattcaa attcacaata
     8821 ttatttttat aaactgagat ataccgttct aagcgggatt tgattttctt acgccctaac
     8881 ttcaacaaag taccattatc taatcgaata aaccacgcat atctaggaga aagcgagatc
     8941 tctgccacgt gttgattaat cgggctgagc gtcctattca taattttata ttgttcagca
     9001 atcaacgcgg cactttcttc agttggcccc ctaaatacag gtaaagcttt atctgacgga
     9061 gcgcgaaata tctctccttg aagattaacc aaagcagtat ctccccagta tgccaatgca
     9121 ctatgctctt ctagtataac tttgattcct aatggccaat tacgctgcac ctttgctact
     9181 ctcacccaag gcaactcttg aaaagcattc tttacccgat tcaaatcgac agtaaaaaaa
     9241 ttccctatca ctgcatcacc aacaacacgt tgaatctgtt gtcggttgat atgttgtaat
     9301 gccaacttat ctgattgatc cgtattactt acactttcaa ttcttatttc tttgagatcg
     9361 aatagcggta attttaccat gcttgagaca aggaaataag caacagataa cacaactcca
     9421 gttagaatca agttagccag caaattcaat gccttgtgat tattccacat gggtaagtgc
     9481 taaaatttga actactaact gatcaaacga tatacccgca gctcgtgcag ccattggaac
     9541 taaactatga tcagtcatac caggagatgt attggcctcg agaaaaaagg gacgatgatc
     9601 gtggtcaagt attacatcaa ctctactcca accataacag cccaaaatag tatgtgcctg
     9661 taacgcttgg cgctgtattt gttcggttag atcatctggc aatccgctgg gacaataata
     9721 gcgcgtatca tttgacaaat atttagcctg ataatcatac agatcgccag acacctcgat
     9781 tcttacaagt ggcaatggtt tgcttcctaa aattgcagct gttaattcct tcccttcgat
     9841 aaattgctca gccaatacaa tagaatcgtg atttagcgcc agctggtaag cctcattaaa
     9901 tgcctggggt gttttaatct tactcaaacc aattgtcgat ccttctctag caggttttat
     9961 gataaggggt aagcccaggg tgtcaatcac ttcctcagcc gctttctgtc catctagaat
    10021 taagaatcgt ggagtactaa taccaaccgc ttgccaaatt aatttagtcc gccatttatc
    10081 cattgctaaa gcactagcta gtacaccact tccggtatag ggaattccta atagttctag
    10141 tgcaccttgg atcgaaccat cttcaccaaa acgaccatga agcgcaataa atgcgcgatc
    10201 gaatccttga cgtactaatt cctctagcga ttgttcactg ggatcaaatg gatgggcatc
    10261 aatcccctgt cggataagcg cattgagcac cgcctcacca cttctaaggg aaacttctct
    10321 ctctgctgaa cgcccgccca gcaatacggc aacttttcca gaattaactg tattcattgg
    10381 gttatcacct tagtattacc aatgattctg acttcctgag tcaaactaac accagtttgc
    10441 tctctaacgc gatgacggac ttcagtgata attgtttcaa tatcagcagc tgtggcattt
    10501 cccgtattga taatgaaatt agcgtgctta gttgatatca ttgcaccgcc aatctgcaac
    10561 cccttcagat tggaaaattc gatcaaacgc gccgcataat ctccggctgg atttcgaaaa
    10621 acagaaccgg cattaggata attaagcggt tgagctgcaa tccgtgcagt taatagctcc
    10681 tttatttttt gcttggaaac actaccatca cctctagcca atttaaacca agcaccagca
    10741 aaccactctt catctccttg tttattgtca ttcgattttc tgatcacaga acgatacccg
    10801 atttgataat catcaatatt tttttctatg atgtttcctg agcgctcaat aacttgaacg
    10861 cgttcaacga tattccaggt ttcaccacca tagcagccag cattcatagc aagtgctcct
    10921 cccaccgttc ccgggattcc tgctaaaaac tcagccccga ctaaattact catgcccatg
    10981 aatcgagcca atttagcaca agatacaccg gcccctgcaa aaacactccc cccactacca
    11041 tccatgtggc taataaacaa atcattcaat cttgcgtgca atcccacaat tactccatca
    11101 aacccaccat cccgaaccag cagattactt cccaaaccaa tcatatagag aggtattttt
    11161 tcaggaagca gcgacaagaa tcgtgataaa tcattgcgat cttggggaat ataaaaatac
    11221 ctcgcagtac cacctgcccg ccatgaaaca tgctttccca ttggctcgtt gattttcagt
    11281 tgacctctca gctgacccaa atcgacaata tcctgctcaa tcgaattatc aaaatcaaat
    11341 agatccataa ccgctgcatt caattgcatt aaaacctctt actaaaatac acggaaagtt
    11401 tttattcctc gattatccaa tcgcaaccaa cttttttgca gcttttgaca cccatgcggc
    11461 aacctttcca attgagcctg ctcccataat aagcacaaca tctctatctt caacaatccc
    11521 atgaattaca ctgggtaact catctggcat ctccacatag atgggttcta attttcctaa
    11581 aacacgaatc gcccgagtta atgatttact gtctgccgca acgatcggct cttcccctgc
    11641 ggcatatact tctgtgagca acaaaatatc tactgtggat aataccttaa caaaatcctc
    11701 aaacacatca cgtgtacgtg tatagcgatg gggttgaaat gccaatatga gcctcctccc
    11761 tggaaaagcg cctcgggtcg ctgagattgt cgcagcgatt tcagcaggat gatggccata
    11821 gtcgtcaatt aaggtaaaac ttcctttacc ggggatttta atatcgccat aacgttggaa
    11881 tcgccgctca actcctccaa aagttgcaag cgctcgaacg atagcagcat caggcacacc
    11941 gatttcatta gcaatggtaa ttgcagcaag cgcattctgc acattatgta accctggtaa
    12001 atttaacgta atatcaagct ggcgcgcggt tccattaaca ccaatattta ctcgaaaaca
    12061 cattctgcca ttgtcgtgtt gaactgaaga tgcgctgact tgtgcatctg gtgataaacc
    12121 ataggttgta attggtttcg ttatattggg aagaatttca cgaatgtttt gatcgtcaat
    12181 acatagtaca gccataccgt aaaaagggag atgttcaata aattccacaa acgtttgctt
    12241 gagccttgca aaatcatggc catacgtgga catatgatct gcatcgatat tcgttacaac
    12301 tgttaagatg ggttgcaaat ataaaaatga agcatcagat tcatctgctt ccacgacaat
    12361 gaattcacct ttgcccagct tagcatgact gcctgcagct tcaagctttc cgcctataac
    12421 aaaggtaggg tcatatccag cttctgccag aatactcgca accaaactcg tcgtcgttgt
    12481 ttttccatgt gttcctgcaa tggctattcc ttgtcgcaaa cgcagcaatt ctgcaagcat
    12541 catcgctctc ggtacaaccg gtatatggcg ctcacgcgct gcgataattt cagggttatc
    12601 tgctttaaca gccgttgagg ttacgaccac atcaacattt gctacatttt ctttgtcatg
    12661 cccatagtaa atcaaagcgc ctaatttctg taagcgttca gtcgtcatgt tttccattag
    12721 gtcagagccg cttatcttgt aacctaagtt aattaacacc tcagcaatac cgcccatacc
    12781 ggcaccacca attccaacaa aatgaacaga tttaacctta tgtttcattg ttcgctttct
    12841 ccattgcgat acatacttgc gcgacttgtt gcgcagaatt gaaatttgcc aaatttcgtg
    12901 caatttttgc catatcaagc aactgcgctc gagtcaattc cattattttg tcggctaaac
    12961 tatcagcgtt caatttatgc tgtggtagca acactgccgc accaggtttg gaaagatatt
    13021 cagcatttgc cgtttgatga tcatcaactg cataaggaaa aggtattaaa atgcttgcaa
    13081 caccaactgc tgctaattca gtaatcgtta gcgcaccggc tctacaaata actaaatcac
    13141 actcggcata actggctgcc atatcatcaa taaaatcgat taactgccct tcaacacctt
    13201 gctcacgata attctctctc aattcatcaa tatgctgcct acctgcttga tgttgcacga
    13261 tgggacgttt tgcttcaggt atacgactaa gcgcctgggg tattacttta ttcaatacac
    13321 tcgctcccaa gctaccccca atgaccaaaa tcttaatagg tccagttcga tctgaaaatc
    13381 gttgctcggg caaaggtaat tctgaaattt tacttcgcac cggatttccc gtgacaacaa
    13441 cacgattatc tttttgagga aatgtaccgg gaaaacctaa taagatttta tcagccactc
    13501 gagcaaggat tttatttgtt aacccagcaa tcgcattctg ttcatgaatg actaggggtt
    13561 tattcaagag aactgccatt aatccgcctg ggaatgcggg atagcctccc attcccaata
    13621 caatactcgg ttgattgcgc gtcaaaattt tgatactttg ccagaaagcg agtagcaatt
    13681 ttcctggcaa tagcaaccag tccattattc tttttcttct aataccagaa aaattaatat
    13741 attcgacagg atagccatat ttaggcacga gtactgcttc cattccgttt tgtgtcccta
    13801 accatacaac ccgccaccca agtgaacgca tataatcggc cacggcaatc ccaggaaaca
    13861 catgccctcc cgtaccccca gccatgatca acagtgtatg tttcacattg acctctaaca
    13921 aaaaacacgc tttgcatcag tcatacggcc attcccctaa ttctttggcg attctcgcta
    13981 tcgattcgca acaaaatggc taatgcaata cagctcacta cgatgctact tccaccgaaa
    14041 ctcatcaatg gcaacgttaa tcctttcgtt ggcaacaccc ccatattaac ccccatattg
    14101 attagcgcct ggagtcctag ccagattccg attccttgag cgaccaaagc cgagaaatag
    14161 ttttccagtg aagctgctaa acgcccaatt tgataggccc ttattaataa gcaagcaaat
    14221 aacatcatta caaccattac gccgataaaa cctaattctt cggcaagtac cgcgagtaaa
    14281 aagtccgtat gcgcttcagg taagtaaaaa agtttctcta cacttccacc caatccaacg
    14341 ccaaaccatt ctcctcgacc aaaagcaatt aatgcatggc ttaattgata acctttaccg
    14401 aatggatcag cccaaggatc cataaaggca atgatacggt ccatccggta aggttcaaat
    14461 aacactaata acaccagacc gattaccaaa atacttgcca gtcccgcaaa tagcttaaga
    14521 ctcattcctc caaggaacat gataaccacc gcaatcgatg tgatgacgac aaaagcacca
    14581 taatcaggct cgcgtagtaa caatacaccg actaccccaa taacaagcca taaaggtaaa
    14641 aaacctcttc gaagatcatt aagatatagg gatttgcgta ccacataatc tgatacataa
    14701 agaatcatca acagcttcat gagttctgat ggctgaaaat tgacgatgac gagcgatatc
    14761 caccgttgac tcccattaac ttcacgtccg attccaggaa tcaggactaa aatgagtaag
    14821 gcaactccac ctaaaaacaa atagggaaca agcgttcgcc acactcgagt aggaatttga
    14881 aatgcaatag aacctaatat caagccgatg atgatatagc tggcatgtcg agttaagtaa
    14941 tgaaaagaac ctgcttcccc atactctgat tcagcaattg cgatggatgc agaataaatc
    15001 ataattaagc cgaggcttat cagtgcca
//
LOCUS       DAOQKQ010000051        14993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_1829_length_14993_cov_5.312759, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000051 DAOQKQ010000000
VERSION     DAOQKQ010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 14993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14993
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1829_length_14993_cov_5.312759"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /locus_tag="PKZ49_04860"
     CDS             <1..1056
                     /locus_tag="PKZ49_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HND35928.1"
                     /translation="LMVYGFFPFFIFGFLFTTFPNWMNGEKIKPSQYLSTCIFMLIGV
                     ILFYSGLFWGISFVLIGIGAMLIGWGVAIVALLTVLFQTKAQDKRHASVASIAFIFGW
                     LGIASYFLWLLTNDVIYLNFARQAGIWFFLLPILLVVSHRMIPFFSSRVLENYVMVRP
                     YWILWVMLACSLGHGLLLLFNQSELTWVVDFVLASCAFYLSIVWGFKRSFSVSLLAVL
                     HISFAWLSIAMLLFALQSLMFWFDNERMMLLGLAPVHALVIGYFSSMVLGMASRVTLG
                     HSGRPLALDSFTWLLFLGFQWATFSRILPDMIPNLSEQYLYWYLVAGSIWLVCFTIWA
                     FRFAPIYWRPRSDGKPG"
     gene            1300..1878
                     /gene="petA"
                     /locus_tag="PKZ49_04865"
     CDS             1300..1878
                     /gene="petA"
                     /locus_tag="PKZ49_04865"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HND35929.1"
                     /translation="MNSRRRFLVVTTSLAGGAACAGIATPFLLSMMPSERAKAAGAPV
                     EVDISKLEPGMLLMVEWRGRVVWILRRTEEMLGTLKKLEDQLADPASDKDQQPTYAKN
                     ETRSIKPEILVVEGVCTHLGCSPVFRKDIAPADLGADWLGGFFCPCHGSKFDLAGRVY
                     KSVPAPTNLAVPPHTYLSDNQLIIGSESKESV"
     gene            1879..3126
                     /locus_tag="PKZ49_04870"
     CDS             1879..3126
                     /locus_tag="PKZ49_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HND35930.1"
                     /translation="MSKQQNSVIQWFDDRFPLLSTWRAHLSEYYAPKNFNFWYYFGSL
                     AVLVLVNQLITGIFLTMSYKPDASMAFASVEYIMRDVDYGWLIRYMHSTGASMFFVVV
                     YLHMFRGMMYGSYRKPRELLWLIGMFIFFILMSLAFTGYILPWGQMSYWGAQVIVSMF
                     GAIPYIGDTLSQMILGDFTLSDAALNRFFAYHVVTLPLLLLVLVFVHIVALHEVGSNN
                     PDGIEIKSNLDSKTGTPVDGIPFHPYYSVKDIFGVVAFLIVFCGIIFFAPEFGGYFLE
                     YNNFIPANTLQTPDHIAPVWYFTPYYSMLRAVTVNFLGIDAKLWGVILMAMAVVIFCF
                     LPWLDRSPVKSIRYKGPYFKFALTLFVISFFVLGWLGTKAPTPLYTLLAQIFTVIYFA
                     FFLLMPWYSKIDKTKPEPKRVTK"
     gene            3123..3848
                     /locus_tag="PKZ49_04875"
     CDS             3123..3848
                     /locus_tag="PKZ49_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HND35931.1"
                     /translation="MTLIRSFLIIFLFFPIGINAAGVEVQLDKAPIDTRDNDSIKRGA
                     QSFVHYCLTCHGASFMRYNRHQDVGFTNQELLDNIIQTGQKVGDLMVSAMKKNEAEGW
                     FGVAPPDLSVIARSRGADWLYTYLRSFYRDETTATGWNNLIFDKVAMPHVFYELQGQQ
                     ILKVKSLGDGKESKTLELAVPGSIPKAEYDKFVADLVNYMVYLGEPHAAHRVDLGIKV
                     MLFLFAMLGLTYLLYKEYWRDIH"
     gene            3921..4520
                     /locus_tag="PKZ49_04880"
     CDS             3921..4520
                     /locus_tag="PKZ49_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HND35932.1"
                     /translation="MMTLYSTATCPFSHRCRIVLHEKDMDFQIHDVDPNNKPEDLAVI
                     SPYGQVPVLVERDLVLYESNIINEYIDDRFPHPQLMPAEPVMRARARLLLHRFEQELF
                     CHIEVAEQNDHKLTDSARKIIAENLTMIASLFDKQKYMLGDEYSMLDVAIAPLLWRLD
                     YYGIRLPKQAAPLLKYSERLFSRPLFIDALTPSEKMMRK"
     gene            complement(4543..5481)
                     /locus_tag="PKZ49_04885"
     CDS             complement(4543..5481)
                     /locus_tag="PKZ49_04885"
                     /inference="COORDINATES: protein motif:HMM:NF021446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HND35933.1"
                     /translation="MLNKQEALQAIIDIVRNHQLSNKEVIAALTDTKNITKQRTTGVL
                     SRIFGYIGSILVFSGICIFIGMRWEQLGSVERITATLGIGFMLFLIAVAVMDNPKYDR
                     TATPLLLIAALLQPTGIFVLLDEFSRGDDPLYGVLFMSVIMFIQQGFIFLQKGRTTLA
                     FTTILFGSMFIITTLELLEVDGNVISFMIGLSLMFISWALGHSPHRAISSFWYFFGSI
                     SLLISAFDFLKNQTYEVLFLGLAAIIIYVSIAAQNRTLLLVGTLAMLSYISHYTYIHF
                     NDVLGWPIVLIICGVAFIAISAVAMKINAKFMSTPS"
     gene            complement(5490..6080)
                     /locus_tag="PKZ49_04890"
     CDS             complement(5490..6080)
                     /locus_tag="PKZ49_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35934.1"
                     /translation="MKQNFLKENFALIIGLGLPVVLVIGFFFARYLPQQYSDEPPSYE
                     LVFSVMRYDNYSPFHVDFIVKDQKLYMKLMNKKEHEGANVRELFIYNGQKGSTRKIHY
                     SLPDHLVNLQNEILVKEFSAHVIDTNSKSPDGYELEPASYRSRGLLGELFGSNTGKYF
                     HRVKKAGGKSFPISDYGSEFSYGNLSFIGWIIQSNQ"
     gene            6404..8797
                     /locus_tag="PKZ49_04895"
     CDS             6404..8797
                     /locus_tag="PKZ49_04895"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HND35935.1"
                     /translation="MFHHIQVPIDGMTCAACAARLEKNLNKLQGVNASVNFANEKANI
                     DYEESLTQPQAIIQSIKDSGFNVLPQTLQLKIDGMTCAACSGRVESALNKLPGVTANV
                     NQATEVAYITYTPGIVSLENLIEAVTDSGYHADKLNQANRDKENSRKQAIYQDDLRLF
                     WFSAILTIPLMLQMLTMFSDDHSEWLPRWLQWLLATPVQFWIGFRFYKGAWYALKGGA
                     ANMDVLIVLGTSMAYLFSTVVTLYGLEQHVYFEASAAIITLVLLGKLMESRAKNKTST
                     AIEELIRLQPTIAHVQKENGEILDLDINNLSVNDIFIVRPGESLPVDGIVIEGDSAIN
                     EAMLTGESLPVTKRVGDNVYAATINQQGLLKCRATSVGSETQLAEIIRLIERAQGSKA
                     PIQRIADKIAGIFVPIVMGISLLTLSLTWWLEGEFVLALINAVSVLVIACPCALGLAT
                     PTAIMVGTGRSAQMGVLVKNAAALEHAEKIQVLVVDKTGTLTMGKPVVTNIIPIGKTD
                     QTTLLQIAALIEQNSEHPLAKAILDQAIGINKLEQKITNFQAITGRGVKAEIDGIAYI
                     LGSPKFLGEYGVENDHIEIKKLQLEGKTLVGIAMQLDHRYQSLGYFAIADQLRETSKQ
                     AIQRIQAMGIEVIMLTGDNAATATTIAKQAGIMQYRAEVLPQDKAAVVSEIKSQGKYT
                     GMIGDGINDAPALAAADVSFAIAAGSDVAIEAADITLMRNDLMSVADAIALSRATLRK
                     IRQNLFFAFIYNVLGIPLAAVGMLNPVIAGAAMAMSSVSVVSNSLLLKRWNMSRYNK"
     gene            8845..9054
                     /locus_tag="PKZ49_04900"
     CDS             8845..9054
                     /locus_tag="PKZ49_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HND35936.1"
                     /translation="MESKTVYIKGMNCMGCVSSIKNVLEKLPGVESAEVSLEDACVTI
                     HYDATSIGIDQLKKAIDNAGFEAIT"
     gene            complement(9063..9794)
                     /locus_tag="PKZ49_04905"
     CDS             complement(9063..9794)
                     /locus_tag="PKZ49_04905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HND35937.1"
                     /translation="MKIFWIVSALLILNACASTSRSIQDNPTLPIESSNNEIALDNIN
                     RSVPTSTATEVEQNNPTVALTKGEDRVTSSHKATSKPEWPIETGVATYYANQMEGQLT
                     ASGELYDSAQLTAAHRTLPIGSQVRVTNAKTKQMVIVRINDRWGGGGDRIINLSKAAA
                     TELGFGSAGMLTVTLSVESIPSGSNQQFSEEKPLQAKQLPSRIADDSSANHPKLRICQ
                     NEANILGLTGEFYRYHVSACLGRNK"
     gene            complement(9908..11980)
                     /locus_tag="PKZ49_04910"
     CDS             complement(9908..11980)
                     /locus_tag="PKZ49_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HND35938.1"
                     /translation="MPILRTPLLFKILSAIVLTHLATIVLAAHELNNVPQQKTNNLMD
                     MDIEKLMSLEVTTVSRRSQKLAEVASAIFVITQDDIRRSGVTSIPEALRMAPGVEVAR
                     IGTDKWAIGIRGFNGRFADKLQVLMDGRSVYNPLFAGVQWEQQDTLLEDIERIEVIRG
                     PNASVWGANAVNGIINIITKKAAQTQGLLLTAGGGSFERGFFGARYGGTINDNTHYRF
                     YTKGFTRDPMNSISGANANDAWHSARGGFRLDHSRGIDQFTAQGDIFYNAIGDTFDKT
                     LLGPPIIEPTAARGHNEGGNIRLRWDRTLSEKSSIMFQTYYDRVDYKLLTSTQFRAES
                     FDIDFQHRFPLWNRHDLTWGANYRLYHNKVFDTEVIALSPRQQTNHLASVFVRDEMTL
                     IPEYLRFTLGTRIDHNDFTGFEIQPNARLMWTPNPENSLWASVSRAVRTPSRAENDIT
                     LNTRILNSIPGTNTPIPFPVLAQIAGNSNYNSEKMIAYELGYRHQISQTASIDIAGFF
                     NDYSQLRDLASPAFKDISVHSSFPLHLIYPVGLNNNASGHTYGVELSADWKPTQRWRL
                     QGNYSYLHMHINSNSTFKSIDPTTGGADKVSPNHQISFRSHYDLTERMQFNLWLRYIS
                     GIDFYQIPGYVTMDTKIAYKPIKNLEVFLVGQNLFSENHREFVSDFIPSLPLYIPRGI
                     YVGGEWRF"
     gene            complement(11982..12500)
                     /locus_tag="PKZ49_04915"
     CDS             complement(11982..12500)
                     /locus_tag="PKZ49_04915"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HND35939.1"
                     /translation="MQQLLLKAWLLLLLIIPSPSIADTLDEYQVKAAFIYNFIAFTQW
                     PYEINQPLNFCIYGEDYFGQEIDHLQVKQINNQRIHVLRFSQLEEIKVCQVLFISKSN
                     ISSLPSILAFIQGQSILTIADTPDAAIKGIMINMIVAQNRILFEINLQSARNAKLDIS
                     SKLLQLAMKVYQ"
     gene            complement(12618..14537)
                     /locus_tag="PKZ49_04920"
     CDS             complement(12618..14537)
                     /locus_tag="PKZ49_04920"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HND35940.1"
                     /translation="MKVADIKQLNISALLPSPKGVALALLEVCRKEDATLGEITKLIQ
                     TDPALSGRLIHKANLASQSARPITSVTEAVSRVGLTVVKQLALGFSLIDQYQGGPCKG
                     FNYQQFWSHSLLMAIAMQELGKSVRVCPPDELFACGLMTRIGCLALATIYPGKYTEIL
                     EKQDTNISLPLLEQQFLETDHNQLTAAMLINFGVPNIMVEPIYYHESPHESGFSEGSR
                     PYQIVHLLYLAKQIADLGISPESEHSKRTADLMLLGSKIGLDTESFGQFIDQIMHNWQ
                     QWGEMLKLPAVAPPSFQKMASTPAPRPEESIDAATLRVLLVEDELSSRLLTEGILNNI
                     LGHTVHTAVNGKEALSLAIEVQPHIIITDWIMPAMDGLELTRAIRSTDWGQNIYIIML
                     TSLENEEDIVEAFDAGVDDYVSKPINLRAFRARLRAAWHYRKLQESWERDREQLKRFA
                     AELAVTNRKLEHIALTDMLTELPNRRAGMNVLSETWSNSERANQSMAVMLIDIDYFKK
                     INDTYGHATGDRVLIEIATLIRSNARKGDSFCRMGGEEFLVICHSGCIEMKSTVQFAE
                     RLRQYTKSHPIKIDENNIEVTISIGIAQKETSVENPDQLINLADKALYAAKNAGRNKV
                     FLAAENKLINCSNQS"
     gene            complement(14518..>14993)
                     /locus_tag="PKZ49_04925"
     CDS             complement(14518..>14993)
                     /locus_tag="PKZ49_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HND35941.1"
                     /translation="ETGLPPHKLTLEVTEGALMEYSENTLATLRELRKNKIHIALDDF
                     GTGYSSMNYLKRLPIDSIKVDQSFVSGLLDDKDSLAIVRSIVTLSKNLGFHITAEGIE
                     TLNQAEILKFLGCETLQGFYFSKPLHKNDMLQLSNKSWDIDAVNPLGYHYESRRY"
BASE COUNT         4308 a         2815 c         3197 g         4673 t
ORIGIN      
        1 ctaatggttt atggattttt tccttttttt atctttggtt ttttatttac aacctttcct
       61 aattggatga atggagaaaa aattaaacct agtcagtatt tatcaacttg tatttttatg
      121 ttgatcggag tgattttatt ttattctgga ttattttggg gaatatcgtt cgttttaatc
      181 ggtataggcg ctatgttgat tgggtggggg gtggctattg ttgcattact aacagtgctg
      241 ttccaaacaa aagcgcaaga taaacgtcac gcaagcgtgg caagtattgc attcattttt
      301 ggatggctag ggatagcatc gtattttctc tggcttttaa caaatgacgt tatatacctc
      361 aattttgcac ggcaagctgg aatttggttt ttcttactac cgatactatt agtcgtttca
      421 caccgaatga ttcctttctt ctcgagtaga gtgctagaaa attatgtgat ggtcaggcct
      481 tattggattt tatgggtaat gcttgcatgt tccttagggc atggtttgct gctgttattt
      541 aatcaatccg aattgacctg ggtagttgat ttcgttttag ctagttgtgc tttctatctg
      601 tcgattgttt ggggatttaa gcgtagtttt agcgtcagct tgttagcagt attgcatatt
      661 tcgtttgctt ggctaagtat cgcaatgctc ttatttgcgc tacaaagttt gatgttttgg
      721 tttgataacg aacgcatgat gttgttgggt ttagcacctg ttcatgcctt agtaattggg
      781 tatttttcga gtatggttct cgggatggct tcaagagtaa cattgggtca ctcaggtcga
      841 ccgcttgcat tagattcgtt cacttggttg ctttttcttg ggtttcagtg ggcaactttt
      901 tctagaatat taccagatat gatccctaat ttatctgaac agtatctata ctggtattta
      961 gtcgctggaa gcatttggct agtttgtttc actatctggg cctttcgttt tgctcctatt
     1021 tattggaggc ctcgaagtga tggtaaacct ggttaatcgt aaagttaaat taattaatcg
     1081 tgtgtgaaat tgtatagctt acctatattg tgtttagggt taacatgtta caataccctg
     1141 gttacggtgt aagattaatt tgaataagaa ataagataaa agttcttaaa ttaattcgta
     1201 atatataaat agtctataaa atcagaagaa taaattggtt gttttgggta aaggtagcta
     1261 taaattaatt cttaaaattt ggttgatggt agaggtgaaa tgaatagtcg acgacgattt
     1321 ttggtagtga cgacatcttt agcaggtggt gccgcatgtg cagggattgc tacaccgttt
     1381 ttgctgagta tgatgccgag tgaacgtgcc aaggccgctg gagctccagt agaagttgat
     1441 ataagtaaac ttgaacctgg tatgctgtta atggttgaat ggcgtggaag agtagtatgg
     1501 attctcagac ggactgagga aatgttggga acattgaaaa agttagaaga tcaattggct
     1561 gatcctgctt cagacaaaga tcaacaacca acgtatgcta aaaatgaaac acggtctata
     1621 aaacctgaga tattggtagt tgaaggtgtt tgtactcact taggttgctc tcccgtattt
     1681 aggaaagata tagcgcctgc ggatctaggg gctgattggt tgggtgggtt cttttgccct
     1741 tgccatggtt ctaaatttga tttggctggt cgtgtctata aaagcgttcc agcaccaact
     1801 aatttagctg tgccaccgca tacttattta agtgataatc agcttattat cggctctgaa
     1861 agtaaggaat ctgtataaat gagtaaacaa caaaattctg tcattcagtg gtttgacgat
     1921 cgttttccgt tgctttccac atggagagcg cacctatccg aatattatgc tcccaagaat
     1981 tttaattttt ggtattactt tggttcatta gcggtattag tgcttgtcaa tcaactcatt
     2041 acaggtattt tcttgacgat gagttacaag cctgatgcaa gcatggcatt tgcttctgtt
     2101 gaatacatta tgagagatgt tgattatggt tggttgattc gctatatgca ttcaactggc
     2161 gcatcaatgt tttttgtagt cgtatacttg catatgttcc ggggcatgat gtatggctct
     2221 tatcgcaaac cgagagaact cttatggtta atcgggatgt ttattttctt tatattaatg
     2281 agcttggcct tcacgggtta tattttacct tggggacaga tgtcgtactg gggcgcacaa
     2341 gttattgtga gcatgtttgg cgctattccc tacatcggtg atacgctctc acaaatgatc
     2401 ctgggtgact ttaccctctc agatgctgcg ctaaatcgtt tctttgctta tcatgtagtg
     2461 acgttgccat tgttattgct tgtattggtt tttgtgcata ttgttgcttt acatgaagta
     2521 ggatcgaata atcctgatgg aattgaaatt aaatcaaatc ttgattctaa aacaggtacc
     2581 cctgtcgatg gaattccatt tcatccttac tactcagtta aagatatttt tggagtcgta
     2641 gcatttctaa tcgttttctg tggcattatc ttttttgccc ctgaatttgg tgggtatttc
     2701 ctggaataca ataattttat cccagccaat acactacaaa cacccgatca tatcgcacct
     2761 gtttggtatt tcacgcctta ctattcgatg ttgagggcag taacggttaa tttcttagga
     2821 attgatgcta aattatgggg tgttatctta atggcgatgg ctgtggttat tttttgcttc
     2881 ttgccttggc ttgatcgtag tcctgttaaa tcgattcgtt acaaaggtcc ttatttcaaa
     2941 tttgccttaa cgctttttgt aattagcttc tttgtattag gttggttggg taccaaagca
     3001 ccaaccccat tgtataccct attagcgcaa atttttacgg ttatttattt tgctttcttc
     3061 cttctaatgc cgtggtatag caaaatagat aaaactaaac cagaacctaa aagagtgaca
     3121 aaatgacatt aatacgatcg tttcttatta tctttttatt ttttccaatc ggaattaatg
     3181 ctgctggtgt agaagtacag cttgataaag cgcctattga tacacgtgat aatgattcaa
     3241 taaaacgagg tgcacaatcg tttgttcatt attgcctaac ttgtcatggt gcaagtttta
     3301 tgcggtataa ccgccatcaa gacgttgggt ttaccaatca ggagctgctt gacaatatca
     3361 ttcaaacggg tcagaaagtg ggtgatctaa tggtcagcgc gatgaaaaag aatgaagctg
     3421 aaggttggtt tggagtagca ccacctgatt tatcagtaat tgcgcggtca agaggtgcag
     3481 actggctata tacttattta agatctttct atcgcgatga aacaacggcg acagggtgga
     3541 ataatttgat atttgataaa gttgcgatgc cccatgtttt ctatgaatta cagggacaac
     3601 aaatactcaa agtcaaatca ctcggtgatg gtaaagaatc gaagactctt gaactggctg
     3661 tacctggttc tattccaaaa gctgagtacg ataaatttgt agctgacttg gtcaactata
     3721 tggtttatct tggagaacca catgctgcac atcgtgtcga tttaggaatc aaggtgatgt
     3781 tattcttatt tgcgatgctt ggactaacct atttactgta taaagagtat tggagagata
     3841 tacattaaga atcgaatagc gttaacagtt tgtactcgta tctcttttaa tagcctatag
     3901 tataagattg ctgaaaagtt atgatgacac tctactcaac ggcaacgtgt ccttttagtc
     3961 atcgttgtag aatagtttta catgagaaag atatggattt tcagatccat gatgttgatc
     4021 ccaacaataa acctgaagat cttgcagtaa taagccctta tggacaagtt cccgtgttag
     4081 ttgaacggga tcttgttctt tatgaatcga atattatcaa cgagtatatt gatgatcgct
     4141 tccctcatcc gcagttaatg cctgctgaac ctgttatgag agcaagagcg agattactgt
     4201 tacatcgttt cgagcaagag ttattttgtc atatcgaagt tgctgaacag aatgatcata
     4261 aattaactga ttcggctcga aagatcattg cagaaaactt gacgatgata gcttctcttt
     4321 tcgataagca aaagtatatg ctgggcgacg aatattcgat gctggacgtt gcaatagcac
     4381 ccttgttgtg gcgactagat tattacggta ttcgtttacc caaacaagct gcacctttgc
     4441 ttaaatattc agagcgctta tttagtagac ctctgtttat cgatgcatta acgccatcag
     4501 aaaaaatgat gcgcaaatag cgttaatatt gttgtgtttt tgctagctag gcgtgctcat
     4561 gaatttggcg ttaattttca ttgcaactgc gctaatcgcg ataaaggcga ctccacaaat
     4621 aataagcacg attggccaac ctagtacatc attaaagtgt atgtaagtat aatggctaat
     4681 ataactcagc atagctaaag tacctactag caataacgtt ctgttttgag ctgcaatact
     4741 cacgtagatg attattgcag ccaagcctaa aaatagtact tcataggttt ggttcttaag
     4801 aaaatcaaaa gctgaaatta acaaactgat cgaaccgaaa aaataccaga atgacgatat
     4861 ggctcgatga ggtgaatggc caagcgccca cgatataaac attagcgata atccaatcat
     4921 aaagctaatt acatttccat ctacttctaa taactctagt gttgtgatga taaacatgct
     4981 tccaaaaaga atcgtcgtaa acgcaagcgt tgttcttcct ttctgcaaaa atataaaacc
     5041 ttgctgaata aacatgataa cggacatgaa taaaacccca taaagaggat catctccacg
     5101 tgaaaactca tcaagcaata caaaaattcc agtgggttga agtaaagctg caattagcaa
     5161 taggggtgta gcagttcgat catattttgg attatccata actgcaacag cgattaggaa
     5221 taacataaaa ccgataccca gtgtcgctgt aattcgttcg actgatccta gttgctccca
     5281 tcgcatccca ataaagatgc aaattcctga aaatacgaga atacttccaa tataaccaaa
     5341 gattctagaa agaacccctg tcgttctctg tttcgtgatg tttttcgtat cagttaaagc
     5401 agcgataact tctttatttg ataattgatg atttcttacg atatcgataa tcgcttgtag
     5461 cgcttcttgc ttgttaagca tagatatact cattggtttg attggataat ccaaccgatg
     5521 aaactaaggt tgccataaga aaattcactt ccataatcag aaatcggaaa gctttttccc
     5581 ccagcttttt ttactcgatg aaagtatttt ccggtattac tgccaaatag ctcgcctaat
     5641 agtcctcgtg aacgataact tgccggctct aattcataac catcgggaga tttgctgtta
     5701 gtatcgatca catgcgctga aaattctttc actaaaattt cattttgtaa attgaccaaa
     5761 tgatccggaa ggctatagtg aattttgcga gtacttccct tctgcccatt gtaaatgaac
     5821 aactctcgaa catttgcacc ttcatgctct tttttattca ttaatttcat ataaagtttc
     5881 tgatctttaa caataaaatc gacatgaaag ggtgaataat tatcatagcg catgacgcta
     5941 aataccaatt catagcttgg cggctcatca ctgtattgtt gtggaaggta tcttgcaaaa
     6001 aagaaaccaa tcactaaaac aactggtaac cctaatccga tgattaaagc aaagttttcc
     6061 tttagaaaat tttgtttcat tagaatcctt taatgcaaaa atttaagctt actatgctag
     6121 aaaacaattg aaagtaaaaa aatggagctt attttgtatt aaatcaattt aaaacattac
     6181 agttaggaaa ctaacctgaa ttgataaata attttatgaa gttttattga ttttcaagaa
     6241 tcatgattta gctagtcgtg ctgatcagca ttagcctcca ctcttttagg ttttgaggaa
     6301 tctttaatct ggaatgttta gaatcggttt tgctgtattg aattaccatg ttaaagagag
     6361 tacggtaaca attgatagaa aaatttattg aaataaaact acgatgtttc accacattca
     6421 agtaccaatc gatggaatga cttgtgctgc ctgtgcagcg cgactcgaaa aaaatttaaa
     6481 taagttacaa ggagtcaatg cgtccgttaa ttttgctaat gaaaaagcaa acatcgatta
     6541 cgaggagtcg ttgacgcaac cccaagcgat tattcagtcg attaaagatt caggtttcaa
     6601 tgttttaccg caaacgttac agcttaagat agatggtatg acatgtgccg catgcagtgg
     6661 gcgtgttgaa tccgccttaa ataaattacc gggtgtaacg gccaatgtga atcaagcaac
     6721 ggaagtggcc tatataactt atacgccggg tattgtttca ctggaaaatc tgatcgaagc
     6781 agttaccgat agtggttatc acgcagataa attgaatcaa gccaatcgtg ataaagaaaa
     6841 ttcgcgaaag caagctattt accaggacga tcttcgctta ttttggtttt cagcaatttt
     6901 aacgatacca ctgatgttgc aaatgctgac aatgttctct gatgatcata gtgaatggtt
     6961 accgcgatgg ctgcagtggc ttcttgcaac cccagtccag ttctggattg gtttccgttt
     7021 ttataaaggg gcttggtatg ctctaaaagg gggggctgct aacatggatg tcctcatcgt
     7081 actgggtacc agcatggcct acctatttag cacggtagtt acactctacg gattggaaca
     7141 acatgtttac tttgaagcca gtgcggcaat tattaccctt gtattactcg gaaaattgat
     7201 ggagtcccgt gctaaaaata agacttctac cgcaattgaa gaattgatta gattacaacc
     7261 aacaatagca catgtgcaaa aagaaaatgg tgaaatacta gatttggata tcaacaacct
     7321 aagtgtgaat gatatcttta ttgtccgtcc tggggaaagt ttacctgtcg atggtattgt
     7381 gattgaagga gattccgcaa tcaatgaagc gatgcttacc ggtgaaagtt taccggtgac
     7441 aaaacgggta ggcgataacg tttatgctgc tacgattaat cagcaaggat tattgaagtg
     7501 ccgcgccact agtgttggat cagagacaca gctcgccgag attattcgtt taattgaacg
     7561 cgcacagggg tcaaaagccc ccatccaaag aatcgctgac aaaattgccg gaatctttgt
     7621 accgattgtg atggggatca gtttattaac gttatcgcta acctggtggt tagaaggtga
     7681 atttgtctta gcgcttatta atgctgtttc agtattagtt attgcctgtc cctgtgcatt
     7741 aggtttagcg acgccaacag cgattatggt gggaacgggt cgcagtgcgc agatgggtgt
     7801 attggtaaaa aatgcagccg cccttgagca tgctgagaag attcaagtgc ttgtcgttga
     7861 taaaaccggg accctaacga tgggtaaacc tgtggtaacc aatattattc cgattggaaa
     7921 gactgatcaa acgacgttat tgcagattgc agcgcttatc gagcaaaatt ctgaacatcc
     7981 actcgctaaa gccatccttg atcaagccat cggtatcaat aaactggagc agaaaatcac
     8041 aaattttcag gcaataaccg gtcgcggggt aaaggctgaa attgatggta tcgcatatat
     8101 tctcggctca cccaaattct tgggggaata tggtgttgag aatgatcata tagaaataaa
     8161 gaaattacaa ttggaaggta aaacgcttgt cggaattgcc atgcaactcg atcatcgcta
     8221 ccaaagctta ggttatttcg caatcgctga tcaattgcga gaaacttcaa agcaagccat
     8281 tcaacgcata caagcaatgg gtatcgaagt gatcatgcta acgggtgata atgctgctac
     8341 agcgactacc atagcgaaac aagctgggat catgcagtat cgtgctgaag tgttgccgca
     8401 agataaagct gcggtggtta gcgagataaa atcccaagga aaatataccg ggatgattgg
     8461 agatggcatt aatgatgcac ccgctttagc ggcagcggat gtcagctttg cgattgccgc
     8521 aggatcagat gttgcaattg aagctgcaga tattacactc atgcgtaatg atttaatgag
     8581 tgtcgccgat gcaattgcac tttcgcgcgc tactttaaga aaaattcgtc aaaatctctt
     8641 ttttgctttt atctataatg tgctcggtat tccattagct gcggtaggta tgctcaatcc
     8701 tgttattgca ggtgctgcca tggcaatgag ctctgtgtcg gttgtgagta attctttact
     8761 gcttaaacgt tggaatatga gtcgctataa taaatagtta aaagatagat agttttaggt
     8821 tgaatgatga tgggagtaat gaaagtggaa agtaaaacgg tttatatcaa aggaatgaac
     8881 tgtatgggtt gtgttagtag catcaagaat gtattagaaa agcttcccgg tgttgaaagc
     8941 gctgaagtat cgcttgagga tgcttgtgtt accattcact atgatgcaac ttcaattggc
     9001 attgatcaat tgaagaaagc gattgacaat gcgggttttg aggcaattac ctaaataaat
     9061 ctctatttgt tgcgacccaa gcacgcagat acgtgatatc gatagaactc acccgtcaac
     9121 ccgagaatat tcgcttcgtt ttgacaaata cgcaatttag ggtggttcgc tgagctatca
     9181 tctgctatcc gagaaggaag ttgttttgct tgcagcggct tttcttcgga aaactgttga
     9241 ttgctacctg aaggtataga ttcaacgctt aatgtgacgg ttagcatacc cgcacttcca
     9301 aaaccaagtt cggttgctgc cgctttagat aaattgataa ttcgatcgcc acctcctccc
     9361 cagcgatcat ttatgcgaac gattaccatt tgtttggttt tagcgtttgt gacacgaact
     9421 tgacttccga ttggtaacgt gcgatgcgct gcggtgagct gcgcagaatc ataaagctcg
     9481 ccactggcag ttagttggcc ttccatctga tttgcgtaat aagttgcgac tccagtttca
     9541 attggccatt caggtttaga agtagcttta tgactactcg ttacacgatc ttccccctta
     9601 gttaaggcca cggttggatt attttgttca acttcagtag cagttgaagt gggaacggaa
     9661 cgattgatat tgtcgagtgc gatctcatta ttggagcttt caatcggtaa tgtaggatta
     9721 tcttgaatgc tgcgagaagt acttgcacat gcatttagaa ttaagagcgc actaacgatc
     9781 cagaagattt tcatgactgt tattattgtt ttcactgaaa taagatatta ggctttcaat
     9841 aatacctgat caatggttaa aactcactat taagttcatt catcacactg ggtttatagt
     9901 tatcttgcta gaagcgccat tcgcctccga cataaatacc acgtggaata tacagtggta
     9961 atgaagggat aaaatcggat acaaattcac ggtgattctc gctaaataaa ttttgaccga
    10021 ctaggaatac ttctagattt ttaatgggct tataagcaat tttagtgtcc atcgtgacat
    10081 aacccggtat ttggtaaaaa tcgataccgc tgatatagcg aagccagaga ttaaactgca
    10141 tacgctcagt taaatcgtag tgagagcgaa atgagatttg gtggttaggg ctaactttat
    10201 ctgcgccacc ggtcgttgga tcaatcgatt taaaagtaga gttagaattg atatgcatat
    10261 ggagatagct gtaattccct tgtaagcgcc atctttgtgt cggtttccaa tcggctgata
    10321 attcaacgcc ataggtatgc cctgaagcat tgttatttaa tcctaccggg tagatgagat
    10381 gcaaaggaaa gcttgaatga acagaaatat ccttaaaggc tggtgaagct aaatcacgca
    10441 gttggctata atcattaaag aagcctgcga tatcgattga cgcggtttgg gaaatttgat
    10501 gtcgataccc taattcatac gcaatcattt tttctgaatt atagttagaa ttccctgcga
    10561 tctgagctaa aacggggaat ggaataggcg tgttggttcc tggaatcgaa ttaagtatgc
    10621 gtgtatttaa cgtaatatca ttctcagctc gcgaaggcgt tcgtacggcg cgagaaaccg
    10681 atgcccatag cgaattttca ggattcggcg tccacatcaa ccgagcattc ggttgaatct
    10741 caaacccggt aaaatcatta tggtcgattc gtgttcctaa tgtaaatcgc agatattcgg
    10801 gtattaaagt catttcatca cgcacaaaga cgctcgcaag atgattcgtt tgctgacgtg
    10861 gggaaagtgc gatcacttcg gtatcaaata ctttattgtg ataaagccgg taattagcgc
    10921 cccaagtcaa atcatgacgg ttccaaaggg gaaatcgatg ctgaaaatca atatcaaagc
    10981 tctccgcgcg gaattgagta gacgttagta atttataatc aacccgatcg taatacgttt
    11041 gaaacataat ggatgatttt tcagaaaggg tgcgatccca gcgtaaccgg atgttacccc
    11101 cttcattatg tccccgtgca gcagttggct cgataattgg gggacccaat agcgttttat
    11161 caaacgtatc accgattgcg ttataaaaaa tatccccttg cgcggtaaac tgatcaattc
    11221 ctcgagaatg atctaaacga aatccacctc gggcactgtg ccaagcatca ttggcattag
    11281 ctccggatat ggaattcatt ggatcgcgtg tgaatccttt cgtataaaaa cgataatggg
    11341 tattatcatt gattgttccc ccatagcgtg caccaaagaa accgcgctca aagctaccac
    11401 caccggccgt caacaatagc ccttgggttt gagcggcttt tttagtaatg atattgatga
    11461 taccattgac ggcattagcc ccccagacag aggcgttcgg tccacggatg acttcgatcc
    11521 gctcaatgtc ttcgagtaaa gtgtcttgct gctcccactg aacgcctgca aataaagggt
    11581 tgtacacact tcggccatcc atcagtactt gtagtttgtc ggcaaaacgt ccattaaatc
    11641 cgcgtatacc aattgcccat ttatcggtac caatccgtgc tacttctaca ccaggcgcca
    11701 tccgtaatgc ctcaggaata ctggttacac ctgagcggcg aatatcatct tgggtgatca
    11761 caaatatagc tgatgcaacc tctgcaagct tttgcgagcg ccttgagacg gttgtgactt
    11821 ctaaactcat cagcttttca atatccatat ccatcagatt attggttttt tgctgtggaa
    11881 cgttgttcaa ttcatgtgct gctagaacta tcgttgccag atgagtcaat acaattgctg
    11941 agagtatttt aaacaatagg ggggtacgca atattggcat gttattgata taccttcata
    12001 gcaagttgta ataattttga gctaatatcc aatttcgcat ttcgcgctga ctggagatta
    12061 atttcaaaca aaatccgatt ctgtgccacg atcatattga tcatgattcc tttgatagca
    12121 gcatcgggag tgtcagcgat ggtgagaatg gattgacctt gaataaaagc tagaatagag
    12181 gggaggctgc tgatatttga ttttgatata aataaaactt ggcaaacctt aatttcttca
    12241 agctgggaaa atcgtaagac atgaatgcgt tggttattaa tctgctttac ttgtaggtga
    12301 tcgatttctt gtccaaaata atcttcacca tagatacaaa aattgagggg ttggttaatt
    12361 tcataaggcc attgggtaaa ggcaatgaaa ttgtaaataa atgcagcttt tacttgatac
    12421 tcatcaagcg tatctgcgat tgaaggggag gggattataa gcagtaacaa taaccatgct
    12481 ttcaataata attgttgcat tgttattgag ttattcctcg tgctattttc tattaattga
    12541 gcaagcatcg atgaatctaa tatgtattaa atgactatgc ggctatatct tcgctacact
    12601 atgtagcaat atccgcatta actctgattg ctacagttaa tcagcttatt ctcagccgct
    12661 agaaatactt tgttcctacc cgcatttttg gcagcataga gtgctttatc cgccaaattg
    12721 attaactggt ctggattttc aacactagtt tctttttgag caataccgat actaattgtg
    12781 acctcaatat tattttcatc gattttgatt gggtgagact tagtgtactg acgtaatcgt
    12841 tctgcaaatt gcacggtcga tttcatttca atgcagccac tgtggcaaat aactaaaaat
    12901 tcttcccccc ccatgcggca gaatgaatcc cctttacggg catttgatcg gattaacgta
    12961 gcaatttcga tcaacacacg atcacctgtc gcatggccat aagtgtcatt aatcttctta
    13021 aaataatcga tgtcaattaa catgacggcc atggattgat tcgcgcgttc actattgctc
    13081 caagtttcag atagaacatt cattcccgca cgacgattag gcagttctgt tagcatgtcc
    13141 gtcaaggcaa tgtgctctaa ttttcgattg gttactgcca attctgctgc aaaacgtttg
    13201 agttgttccc tgtcacgttc ccaagattct tgtagtttcc gataatgcca agcggctcga
    13261 agtcgggcac gaaaagctct aagattgatg ggcttagata catagtcatc tacaccggca
    13321 tcaaatgctt caacaatatc ttcttcattc tcgaggctag tgagcatgat aatgtaaata
    13381 ttttgccccc aatccgttga acgaatagcc cgagttagct ccagcccgtc catggcaggc
    13441 ataatccaat cagtaataat aatatggggc tgaacctcta tcgcgagtga caatgcttct
    13501 ttaccattaa ctgccgtatg gacagtatgt ccgagtatat tgtttaagat gccctcggta
    13561 agcaggcggc tggacaattc atcctcgacg agtaaaacac gtaacgtagc agcatcaatc
    13621 gattcctcgg gtctaggtgc tggggtgctc gccattttct ggaaagaagg tggtgcaact
    13681 gctggaagtt tgagcatctc accccattgt tgccagttat gcataatttg atcaataaat
    13741 tggccaaacg actcggtatc cagcccaatc ttactaccta ataacattag atcagcggtg
    13801 cgtttactat gctcagattc tgggctgata cctaaatccg caatttgctt ggcaaggtag
    13861 agaagatgaa cgatttgata agggcgcgat ccttcagaga aacccgattc atgaggtgat
    13921 tcgtggtaat agatgggctc aaccataata ttgggaacac caaaattaat caacatcgct
    13981 gcagttaatt gattatggtc tgtttccaaa aactgttgtt ctagtaacgg aagtgatatg
    14041 ttggtatctt gtttttctag aatctcagta tatttaccag gataaatcgt tgcgagcgcc
    14101 aagcaaccaa ttcgtgtcat taaaccacag gcaaacaact catcaggggg acaaactcga
    14161 actgatttac cgagttcttg catggcaatt gccattaaca gggaatgtga ccaaaattgt
    14221 tgataattaa aacctttaca aggcccacct tgatattggt caatgagcga gaagcccaag
    14281 gctaattgtt tgacaacggt caatccaacg cgagaaactg cttctgttac cgaagtgatc
    14341 ggacgtgcag attggctagc aaggtttgct ttatgaatca atcgtccgga taaagcggga
    14401 tcggtttgaa tgagtttagt gatttcgcct aatgtggcgt cttcttttcg gcaaacttca
    14461 agcaatgcaa gtgcaacgcc tttggggcta gggagcaggg cgctgatatt gagttgctta
    14521 atatcggcga ctttcataat gatagcctag gggattcaca gcgtcgatat cccaagattt
    14581 attcgagagt tgaagcatgt cattcttatg cagcggctta ctaaaataga aaccttgcaa
    14641 tgtttcacaa cctagaaatt ttaatatttc agcttgatta agtgtttcga taccttctgc
    14701 ggtgatatga aatcctaaat tcttagatag cgtgacgatt gatctaacaa ttgccaagct
    14761 gtctttatca tcgagtaaac cactcacaaa actttgatcc actttgatgc tgtcgattgg
    14821 taagcgcttt agataattca tggaggagta tcctgtacca aaatcatcga gtgcaatgtg
    14881 aattttattt ttacgtaact cacgcagcgt agcaagggta ttttcactat actccatcaa
    14941 tgcgccttca gtaacttcaa gggtcaattt atgaggcggc aaccctgtct cag
//
LOCUS       DAOQKQ010000052        14876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_1863_length_14876_cov_5.736388, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000052 DAOQKQ010000000
VERSION     DAOQKQ010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 14876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14876
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1863_length_14876_cov_5.736388"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="PKZ49_04930"
     CDS             complement(<1..772)
                     /locus_tag="PKZ49_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HND35942.1"
                     /translation="MKLFEFAPQYIRAIHPYQPGKPISELVRDLGLEKDSVIKLASNE
                     NPLGTSPLALEAMSVALNEIARYPDGSGYELKNALAEHYAVSVDQIVLGNGSNDVLEL
                     AARIFLKPGTAAVYSQHAFAIYPLLAQALGAQGISVPARRYGHDLVAMLNAITPETRI
                     IFIANPNNPTGTLCDPNELQRFVERVPQNVLVILDEAYDEYLPEANKANSIGWLKKFP
                     NLLITRTFSKAYGLAGIRVGFAFTHPELADLMNRIRQPF"
     gene            complement(783..1853)
                     /gene="pheA"
                     /locus_tag="PKZ49_04935"
     CDS             complement(783..1853)
                     /gene="pheA"
                     /locus_tag="PKZ49_04935"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HND35943.1"
                     /translation="MAEQLNQLRAKIDAIDNELLELMNARAKLAQAVGHQKGNAVTYR
                     PEREAQVLSRLQQLNAGPLSNERITALFTEIISACRAMEAPLTIAYLGPPGTFSEEAV
                     SKRFGHAVVTIACHSIDDVFRQVESGSAHYGVVPVENSTEGAVGRTMDLLVQTPLVVC
                     GEIELPVHHCLMAKHSDLTQIQKIYSHPQSFGQCIGWLNHQSAQLKSVAQINVASNAD
                     AARLAASDPHAAAIASSKAAEIFELTICARNIEDDPNNTTRFLVMGSQLVPVSGKDKT
                     SLAISTHDRPGAVYELLAPLAKYQVSMSRFESRPSRKGLWEYMFFIDLEGHQDNNNIA
                     SAIGELRDKATFLKILGSYPAA"
     gene            complement(1893..3083)
                     /locus_tag="PKZ49_04940"
     CDS             complement(1893..3083)
                     /locus_tag="PKZ49_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HND35944.1"
                     /translation="MAKKAQQVFKILTLNQISSVGLKQFPADQYLIGHDLVDPDVILV
                     RSHNMLDMDIPKHVIAIGRAGAGTNNIPVDAMSQRGIPVFNTPGANANAVKELVLAAM
                     LIASRNLVSAFQFVEGLQGDNQKLNELVEANKKHFSGIELPGRTLGIVGLGKIGRLVA
                     DAALKLGMKVMGYDPKISVDAAWSLSAEVQRAQSIEDLLRHSDFISLHVPLADSTRHL
                     INEQLIHYFKREVILLNFSRDEIVSEAAVLAGIKSGKIKYYVCDFPSQHLQNEKGVIT
                     LPHLGASTREAEENCAVMVVKQTIDHLQNGNIKNTVNFPDVIMERESPYRVAVANANV
                     PNLLGQISTCMATAGLNIHNMTNKSRGEMAYTLVDVDSPVSKQVIKAISLITGVLKVR
                     YLPV"
     gene            complement(3076..4179)
                     /gene="serC"
                     /locus_tag="PKZ49_04945"
     CDS             complement(3076..4179)
                     /gene="serC"
                     /locus_tag="PKZ49_04945"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HND35945.1"
                     /translation="MNKVYNFSAGPAVLPQEVLEQARDEMLDWHESGMSVMEMSHRGE
                     EFMSIIAKVEADLRDLVKIPNHYKILFLQGGASSQFAMVPLNLLQTKKEADYVNTGQW
                     SQKAIEEASKYGTVNIAASSVGNHFTGIPEIDTWKMSRNAAYLHYTSNETIGGVEFNW
                     IPDLSKIAGGSTDIPLVADMSSNFLSRPVDISRFGLIYAGAQKNIGPAGLVLVIIRED
                     LVQAPTAGTPTLFSYQAHVQHDSMYNTPPTYAIYMMGLVLEWLKKQGGVPAIEALNLA
                     KSKLLYDLIDASHLYQCPVALQDRSRMNVPFTLRQSALDIEFLKQAKARGLVQLKGHR
                     SVGGMRASIYNAMPYEGVKALVTFMKEFENQYG"
     gene            complement(4216..6762)
                     /gene="gyrA"
                     /locus_tag="PKZ49_04950"
     CDS             complement(4216..6762)
                     /gene="gyrA"
                     /locus_tag="PKZ49_04950"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HND35946.1"
                     /translation="MNQFAKETLTISLEEEMRRSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLFAMHELSNDWNRPYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQNFSLRYVL
                     IDGQGNFGSVDGDNAAAMRYTEIRMAKIAHELLADLDKETVDFGPNYDGSEREPLIFP
                     AKIPNLLINGSSGIAVGMATNIPPHNLSEVIDACLRLLNNPETSIDEIIECIQAPDFP
                     TAGIIYGTSGIRDGYLTGRGRVVMRARTHFEDLEKGSRQSIIIDELPYQVNKANLLIR
                     IGELVRDKKIEGISDLRDESDKSGMRVVIELKRGEIPEVILNNLYKETQMQDTFGINM
                     VALIDGQPRLLNLKQLLQAFLRHRREVVTRRTVYELKKARERGHLLEGLAVALSNVDE
                     IIALIKAAATPAEAKKALIGKAWSSQLVAEMLARSAQDTAAFRPIGLGVEFGLFDHGY
                     QLSDVQAQAILDLRLQRLTGLEQQKIVNEYKEVIDQIIDLLDILANADRITAIIVDEL
                     RSIKDQFGDQRRSEIVTNTQELNTEDLITPADVVITLSHAGYIKSQLLDDYRAQKRGG
                     RGKLASATKEDDFIDNMFMANTHDYILCFSSLGRVYWVKVYNVPQGGRVSKGKPINNL
                     LQLEKAEKINAVLPVKAFDDNRYIFMATSLGTVKKTPLSEFSRPRSNGIIAIGLDEGD
                     YLIGVALTEGNHDVMLFSDGGKAMRFSENDVRPVGRSARGVRGMRLGAGQKVISLLVA
                     ENEELTVLTATENGYGKRTPISEYTRHNRGTQGMIAINTSARNGKVVAAQLVKQDDEI
                     MLITTGGILIRTRVSEIREMGRATQGVTLINLDEGEKLVGLERVVEADEE"
     gene            7160..8356
                     /locus_tag="PKZ49_04955"
     CDS             7160..8356
                     /locus_tag="PKZ49_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HND35947.1"
                     /translation="MSYLMNTYQRLPVTFVKGSGVWLWDNDGNRYLDALSGIAVCGLG
                     HCHPALAKALSHQAETLIHTSNLYHIENQELLAERLALLSGMDKVFFCNSGAEANEAA
                     IKLARLYGHHRGIELPTIIVMEKSFHGRTMATLTATGNRKAQAGFEPLLTGFVRVPYD
                     DLVAVEQIAANNKNIVAILVETYQGEGGVNFPQVNYLDGLRRLCNQNNWLLMLDEVQC
                     GIGRTGKWFAFQHSNSSPDVITLAKGLGSGVPIGACIAKGPAAEIFKPGNHASTFGGN
                     PLVCKAALTTLEVIEQDHLMQNAVSMGDLMRNRFHEKLANWSNVIKIRGQGLMLGIEL
                     PIACGELVKEALAAKLLINVTSERVVRLLPALVITQVEAEQIVDKTSSLIDRFLTTQY
                     NNTTPT"
     gene            8384..9298
                     /gene="argF"
                     /locus_tag="PKZ49_04960"
     CDS             8384..9298
                     /gene="argF"
                     /locus_tag="PKZ49_04960"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HND35948.1"
                     /translation="MTPKHFLKINDFTLDEFEYLFERAHWIKNEFKQYRKYWPLTDRT
                     LAMIFDKHSTRTRLSFEAGMCQLGGSVIYLSTRDTQLGRGEPVEDAARVISRMVDIIM
                     IRTFEHSIIERFANHSRVPVINGLTDEYHPCQIIGDIFTFIEHRGSIKGKTIAWIGDS
                     NNVCNTWLQAAEVFDFNVHVSTPPGYEVEPERAGLYGTNHYEEFSSPHDAVKNADLVT
                     TDVWTSMGFEDETEIRKNAFADFCVDDEMMAYAKADALFMHCLPAHRGEEVTNEVLEG
                     RQSVVWDEAENRLHVQKALMEYLLLGKI"
     gene            9350..10570
                     /locus_tag="PKZ49_04965"
     CDS             9350..10570
                     /locus_tag="PKZ49_04965"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HND35949.1"
                     /translation="MKKVKKVVLAFSGGLDTSVILKWLQEVYQCEVVTFTADIGQGEE
                     IEPARAKAQRLGIKEIFIEDLREEFVREYVFPMFRANTIYEGEYLLGTSIARPLIAKR
                     QIEIARETGADAVSHGATGKGNDQVRFELGYYALQPDIQVIAPWREWDLTSREKLLSY
                     AERHGISVEMKQKGGSPYSMDANLLHISYEGRSLENPALEPEESMWRWTQSPEKAPNE
                     SEYIELEYERGDIVAINGKRLTPATILEQLNQLGGKHGIGRLDLVENRYVGMKSRGCY
                     ETPGGTIMLRAHRAIESITLDREVAHLKDELMPRYASLIYNGYWWSPERMALQTMIDE
                     SQVHVNGWVRVKLYKGNVIIVGRDSKTDSLFDTNIATFEDDAGAYNQADAAGFIRLNA
                     LRLRIASKIRAPRK"
     gene            complement(10613..11428)
                     /locus_tag="PKZ49_04970"
     CDS             complement(10613..11428)
                     /locus_tag="PKZ49_04970"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HND35950.1"
                     /translation="MHPMLTIAVKAARRAGNIINQASRNLDILNVTQKSHSDFVSEVD
                     GAAEEAIIKILLDAYPNHAILAEESGSQSGQKQSDYQWIIDPLDGTTNFLHGFPKYCV
                     SIALLHRGVLSQAVIYDPVSNELFTASRGRGAYLNDHRIRVSKRTNLSESLIGTGFPF
                     RDLSFMEAYLAMFKAVIPKTAGIRRPGSAALDLAYVAAGRYDGFWEMGLSPWDIAAGC
                     LLIIEAGGMVSDLEGNDHYLKSGQVVAGNPKIFPQLMEIISPYITDQLIAQNR"
     gene            11468..12202
                     /locus_tag="PKZ49_04975"
     CDS             11468..12202
                     /locus_tag="PKZ49_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HND35951.1"
                     /translation="MIHHSPLDNVRVVLSHTSHPGNIGASARAMKTMGLSSLYLVNPK
                     SFPDNEAYVRATNARDILDQAVICSTLDIALQDCVLAVAMTARARELTHQVIDTRQIA
                     QKLVEIATESPVALVFGRESSGLTTAEVTKCQLTVHIPTSSTYASLNLAAAVQIMCYE
                     LRMGAVTPESTSPPPEVPQLASLNEIELFYQHLEEALITSKFLDPQQPKRLMQRIRRL
                     FSRTALEKEEVNILRGIIHALAKEKR"
     gene            12307..13050
                     /locus_tag="PKZ49_04980"
     CDS             12307..13050
                     /locus_tag="PKZ49_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CADD family putative folate metabolism protein"
                     /protein_id="HND35952.1"
                     /translation="MTTNTIFKHKVSAIINNKHLLKHPFYMAWTEGKLTQEQLRHYAE
                     QYFYNVLAEPTYLSAVHFNTPHGHTEENCGDISVRQEVLKNLIDEEHGSGNHPALWKA
                     FAFALGANDKSLANAAALPETDNLVSTFRDICLNSPFYVGLAALHAFESQVPDIAAVK
                     IEGLAKFYGMNNPKDYEFFSVHQKADIYHSEAEWSIIERFADTPEKQAAVLAATQRAC
                     DALWKFLDGIHENYCANLICDQQTAVTLH"
     gene            complement(13111..>14876)
                     /locus_tag="PKZ49_04985"
     CDS             complement(13111..>14876)
                     /locus_tag="PKZ49_04985"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HND35953.1"
                     /translation="GITVIKGPATLQYGANAIGGVVDISHQRIPDRIPTNGMSGRTDF
                     RFDHNPGEKAGMISLDIGRSMVALHFDYFHRSANNMHIPGSALDESAIRQQFQVEPTT
                     NSNNVIQNSHSHSQGGSAGISLVGKYGYLGGSYHETARNYGIPPGVPGHGDSSRSEQE
                     AIRIDMNQRRYDLEGFWKTYWGALPSIKAKLSYIDYDHKDFDKNISARNSTLTRFGNS
                     VWESRLEMNHQKGDWLDGKFGMQWQNRNFSATGLETFTPSSNVDSIGFFLTETLVVND
                     RTTFELGGRIEHQTTSPKSNELRLAGISSPLPLPNSLNYLTYSLAASLNYEILPLTSF
                     FLNWQRAQRSPDVQELFASGPNFATRSYQLGRMDLNTEQTDHYELGLRYAGNVISFNA
                     NGYYKSVEDFIYLKNQGFFFNFAPDPPRFQLACANLRNCLPIFGYQADTANFWGYEAE
                     ITMRPKIAATYNPRFTFFSDYVRGSFQSGSLGDVPRLPPLRFGAEFGLQWMQWRSLLR
                     YTHALEQNRPGLLETETPGYNRLDLDITYDWKASDRMKILFFAKLSNMTNATIRNSTS
                     FLRSFAPEAGFSSQVGFSAVF"
BASE COUNT         4259 a         3379 c         3081 g         4157 t
ORIGIN      
        1 taaagggctg acgaatacgg ttcattaaat ccgccaactc ggggtgcgta aacgcaaaac
       61 cgacacgaat accggctagt ccataggctt tagaaaaagt acgggtaatc agcaaattag
      121 ggaatttctt caaccaaccg atgctattgg ctttattggc ttcaggtaaa tactcatcat
      181 aagcttcatc taagatcact aatacatttt gaggcactcg ctcgacaaat cgctgaagct
      241 cgttaggatc acacaatgta ccggttgggt tattagggtt tgcaataaaa ataatacgcg
      301 tttcgggcgt aatcgcgttc aacatcgcca ctaggtcgtg accatagcgt cgcgctggca
      361 ctgaaatacc ttgtgcccct agcgcttgag cgagcaacgg ataaatagca aaagcatgct
      421 gggaataaac tgctgccgta cctggtttaa gaaaaatccg tgcagcaagc tctaacacat
      481 cgtttgaacc atttccaagc acaatttgat cgacactcac tgcataatgt tcagccagag
      541 cattcttaag ctcataaccg cttccatcgg gatatctcgc aatttcattc agtgcaacag
      601 acatcgcctc aagtgccaag gggctggttc caaggggatt ttcattcgag gctaacttaa
      661 taacgctatc tttttccaac ccgagatctc gaaccaactc agaaatcggt tttccgggtt
      721 gatagggatg gattgcgcgg atatattgtg gagcaaattc aaagagtttc atgatcagtg
      781 ttctaggctg cggggtatga tcccagtatt tttaagaaag tcgctttatc gcgtaattca
      841 ccgattgcgc ttgcaatatt attgttatct tgatgtcctt caagatcaat aaaaaacata
      901 tactcccaca aacctttacg cgaagggcgg gattcgaatc ggctcatact aacttgatat
      961 ttagcaagtg gcgcgagtaa ttcatacact gcaccaggtc gatcatgggt agaaatggcc
     1021 agcgaagttt tgtctttgcc cgatacggga acaagctgcg aacccatcac taaaaaccgt
     1081 gtagtgttat taggatcatc ttcaatattt cgagcacaga tcgttaattc aaaaatctcg
     1141 gcagctttgc tacttgcgat cgcagccgcg tggggatctg aagcagcaag tcgtgctgca
     1201 tcggcattac tagccacatt gatctgcgct actgatttaa gttgagcaga ttgatgatta
     1261 agccatccaa tacattgccc aaaagactga ggatgtgaat aaattttttg aatttgcgtg
     1321 aggtctgaat gttttgccat caagcaatgg tgcacaggca attcaatttc accacacacg
     1381 accaatggtg tctgtaccag caaatccatg gtccttccca ctgcaccctc agttgaattc
     1441 tctacaggaa ccacaccata atgcgctgag cccgattcaa cttgtcggaa tacatcatcg
     1501 atcgaatggc atgcaatcgt aaccacggca tgtccaaaac gcttagaaac agcctcttcg
     1561 gagaatgtgc ctggtggccc taggtaagca atggttaatg gtgcttccat cgcacggcaa
     1621 gcagaaataa tttcagtaaa taaagcggta atacgctcat tggaaagagg acctgcgttt
     1681 aactgctgca gccgagacaa cacttgcgct tctctttcag ggcgatacgt tacagcattg
     1741 cctttttgat gccccactgc ttgtgcaagt ttggcacgcg cattcatgag ctcaagcaac
     1801 tcattatcga ttgcatcgat cttggcacgt aattgattta attgttcagc cataatgata
     1861 atacgcagta tacgtttttt attgttgtta ttttagaccg gtaagtatcg taccttcaga
     1921 acgcctgtga taagagatat tgctttaata acttgtttag atacaggact atcgacatcg
     1981 accaaagtat aagccatttc acctctcgat ttattagtca tgttatgaat attgaggcct
     2041 gcggttgcca tgcaagttga aatctgacca agcagattag gaacgtttgc attggctacc
     2101 gcaactcgat agggagattc ccgttccatg attacatctg gaaaattaac ggtattctta
     2161 atattaccat tttgtaaatg gtcgatggtc tgcttcacaa ccatgacagc acaattctct
     2221 tctgcttctc gcgttgaagc gcccaaatgc ggtaaagtga taaccccttt ctcgttttgc
     2281 aaatgttgac tcggaaaatc acagacataa tattttattt taccgctctt tattcccgca
     2341 agaaccgctg cttcactcac gatttcatcc cgtgaaaaat tgagcaaaat gacttcacgc
     2401 ttgaaataat gaatcagttg ctcattgatt aaatggcggg ttgaatcagc taaaggaaca
     2461 tgaagcgaaa taaaatcgct atgacgcaat aaatcttcaa tgctctgcgc tctttgtact
     2521 tctgcggaaa gactccaagc cgcatcgaca ctaattttgg gatcataacc catgactttc
     2581 attccgagct ttaaggctgc atccgcaacc agtcgcccaa tttttcctaa tcccacgata
     2641 cccaaagtcc ggcccggcaa ttcaatccct gagaaatgtt ttttattggc ctcgacgagc
     2701 tcattcaatt tctgattatc gccttggagc ccttctacaa attgaaaagc agaaacaaga
     2761 ttgcgagaag cgatcaacat cgctgccaac acaagctcct tgactgcgtt cgcattagca
     2821 ccgggtgtat taaaaaccgg gattccccgc tgactcatcg catcaactgg aatattgttc
     2881 gttcctgcac cggctcgccc aatcgcgatc acatgcttag gaatatccat atccagcata
     2941 ttatgagacc gtaccagaat gacatcaggg tctaccagat catgaccgat taaatattga
     3001 tcagccggaa attgcttgag tcctactgaa gaaatttgat tcagtgtcag aatcttaaaa
     3061 acctgctgcg cttttttagc catattgatt ttcaaattct ttcataaacg tgaccaatgc
     3121 cttaacacct tcataaggca tggcattgta gattgaggca cgcatgccgc ccaccgagcg
     3181 atgacctttc aactgcacaa gccctcttgc tttggcttgc tttagaaatt caatgtctaa
     3241 tgcactctgc ctgagagtaa atggaacatt catgcgcgaa cggtcttgca gggctactgg
     3301 acattgatac aaatgactcg cgtcgattaa atcataaagt aattttgact tggcaagatt
     3361 aagcgcctcg atcgcaggca cccccccttg ttttttcagc cattccaaca caagacccat
     3421 catataaatc gcataagttg gtggtgtgtt atacatcgag tcatgttgca catgcgcttg
     3481 ataactaaat aacgttggcg tccctgcagt aggtgcttgc accagatctt cgcggataat
     3541 gaccaatacc aaacccgcag gcccaatatt tttctgggcc ccggcataga tcaatccaaa
     3601 tcgactgata tccacaggtc gagataaaaa attggaggac atatcggcga ctaacggaat
     3661 atcggtactt ccccccgcaa tttttgataa atcgggtatc caattgaatt ccacgccacc
     3721 aatggtttca ttcgatgtgt aatgaagata cgcagcattg cgactcattt tccaagtgtc
     3781 aatctcaggt attcccgtaa aatgattacc cactgatgat gcggcaatat tcactgtgcc
     3841 atacttgcta gcttcttcaa tcgctttttg ggaccattgt ccggtattga cataatccgc
     3901 ttctttttta gtttgtaata gattcagcgg aaccatggca aattgacttg atgccccacc
     3961 ttggagaaac aaaatcttgt aatgattggg aatcttgacc agatcacgga gatctgcttc
     4021 aactttagca atgatcgaca tgaattcctc gccacgatga ctcatttcca tcacggacat
     4081 accgctttca tgccaatcta gcatttcgtc acgcgcctgc tcgagcactt cctgaggaag
     4141 aaccgcgggc cctgcactaa aattgtaaac cttattcatg ggattcattt tccaagaatg
     4201 agtaagcttg tctcattatt cctcatccgc ctcaacgact cgttccagtc caaccaattt
     4261 ttcaccttca tcgagattga tcagcgttac accttgagtc gcgcgcccca tttcacgaat
     4321 ttcactgaca cgagttcgaa tcaatattcc ccctgtagta atcaacatga tttcatcgtc
     4381 ttgtttaacc agttgtgctg caactacctt accattgcgc gctgaagtat tgatcgcgat
     4441 cattccctga gttccacgat tgtgtcttgt atactcgcta atcggtgttc gtttgccata
     4501 gccattttct gttgctgtta aaactgttag ttcttcattc tcggcaacaa gcaacgaaat
     4561 gactttctgt cctgcgccca atctcatccc tcgtacacca cgagcacttc tacccaccgg
     4621 tctaacatcg ttttcactga agcgcatcgc ttttccacca tctgaaaata acatcacatc
     4681 gtgattacct tcagtgagcg caacaccaat caaataatct ccttcatcca atccaatcgc
     4741 aataatgcca ttgctacgtg gccgtgaaaa ttccgataaa ggggtttttt taacggttcc
     4801 taacgatgtc gccataaaga tataacgatt atcatcaaac gctttaaccg gcaagaccgc
     4861 attaattttc tctgcttttt ccagctgtaa tagattattg atcggtttac cttttgagac
     4921 tcttccgcct tgaggtacgt tatatacttt gacccaataa acgcgtccta agcttgagaa
     4981 acacaaaata tagtcatgcg tattcgccat gaacatattg tcgataaaat catcttcttt
     5041 ggtggcactg gccagttttc cacgccctcc ccttttctgt gcacgatagt catctaaaag
     5101 ttgtgattta atgtacccag catgggatag cgttatcacg acatctgctg gtgtgatcaa
     5161 gtcttctgtg ttgagttcct gtgtattcgt gacaatttca ctgcgccgtt gatccccaaa
     5221 ttgatcttta atggaacgta attcatcaac gatgatggct gtaattcgat ccgcattcgc
     5281 gagaatatcg agaagatcga tgatttgatc aataacctcc ttgtattcat tgacgatttt
     5341 ttgttgttcg aggccagtca agcgttgcaa tcgaagatcg agaattgctt gcgcttgcac
     5401 atcggataat tgatagccat gatcaaacaa accaaattca acaccaagtc ctataggacg
     5461 aaaagcagct gtatcctgtg ctgaacgggc cagcatttcc gcaaccaatt gagagctcca
     5521 ggcttttcct atcaatgctt tctttgcttc tgctggtgtt gctgccgctt taatgagtgc
     5581 gatgatctca tctacatttg aaagtgcgac agccaatcct tctaataggt gcccgcgctc
     5641 ccttgctttt ttcagctcat aaacggtacg acgggtaacg acttcacggc gatggcgtaa
     5701 aaacgcctgg agcaattgct ttaaattgag taatcgcggc tgtccatcga ttaatgcaac
     5761 catattgata ccgaaagtat cttgcatttg cgtttcttta tacaagttat tcagaatcac
     5821 ttccggtatt tcgccacgct tcagctcaat cactacccgc atcccggatt tatccgattc
     5881 atcacgcaaa tccgatatcc cttcaatctt tttatcgcga accagttcac caatccgaat
     5941 cagcaaattc gctttattaa cctgataagg cagctcatcg ataataatgc tttgacgtga
     6001 ccctttctca agatcttcaa aatgagttcg cgcccgcatt acgacgcgcc ctctgccggt
     6061 aagataacca tctctaatgc ctgatgttcc ataaatgatt ccagccgttg ggaaatcagg
     6121 ggcttggata cattcgataa tttcgtcgat actggtttca gggttattaa gcaaacggag
     6181 acaagcatcg attacctcgc ttaaattatg gggagggata ttcgtcgcca tgcccacagc
     6241 aatccctgaa gaaccattga ttagcaaatt aggaattttg gctggaaaaa tcagcggctc
     6301 tcgctcagaa ccatcataat taggaccaaa atcaaccgtt tctttgtcca aatcggccag
     6361 taattcatgc gcaatttttg ccatacgaat ttcggtatac cgcatggctg ctgcattatc
     6421 accatcaacc gaaccgaagt taccttgtcc atcgatgagt acataacgca atgaaaaatt
     6481 ctgcgccatc ctgacaatcg tatcgtagac agccgtatcc ccatgcggat gatatttacc
     6541 aatcacgtca ccgacgatac gcgctgattt tttataggga cgattccaat cattcgaaag
     6601 ctcgtgcatt gcaaataaca ctctgcgatg aactggcttc aaaccatccc taacatccgg
     6661 cagcgccctt ccaacgataa cgctcatcgc gtaatccaaa taagaacgac gcatttcctc
     6721 ttctaggcta atcgtaagtg tctcttttgc aaattgattt attgacattg ggtctgtttg
     6781 attttgaaag tattctcaat ctattttgtt aaagggcaga ttgaaataaa aattaactta
     6841 tcggttaaaa ttaataaaag tggccatcca acaaagaagt aaaatgctac ttgcttaaag
     6901 gttgctatat tttagcacgc cattcccgtc cattttttta taaagtttga agaagcatgc
     6961 agggaaggaa ataatttagg tgacgtggcg ttctacgcca aatcagtgca aagcgtggaa
     7021 atagccaaaa agcgcttgat aaagcctact aaataagtaa ataataaaaa attacttaat
     7081 attgcttgat tttcaatttg ataatacagc atagtttcaa atttctcctt tttaactttt
     7141 tagagatgag taatccactg tgtcttattt gatgaacacg tatcaaagac ttccggtaac
     7201 ctttgttaag ggttcagggg tttggctatg ggataacgat ggtaatcgct atttggatgc
     7261 actatcgggt atcgccgtct gcgggttagg tcattgccac cccgctttag cgaaagcact
     7321 cagtcatcag gctgaaacac tcattcatac ttcaaaccta tatcacattg aaaaccaaga
     7381 attattggcc gagcggctgg ctctgttatc aggaatggat aaagtctttt tctgcaattc
     7441 aggggctgag gccaatgaag cggcaataaa actcgctcgc ctttatggtc atcaccgcgg
     7501 gattgagtta ccgacgatca tcgtgatgga aaaatcgttt catggtcgta ctatggccac
     7561 actcactgcg acgggcaacc gcaaagcgca agctggcttt gaacctttac tcactggatt
     7621 cgtacgagtt ccttacgatg atctcgttgc agttgaacaa attgcagcca ataacaagaa
     7681 cattgtcgcg atcctagtag aaacctatca aggcgaaggg ggtgtcaact tccctcaagt
     7741 taactaccta gatggtctgc gtcggttgtg caatcaaaat aactggctat tgatgcttga
     7801 tgaagtgcaa tgtggcattg gacgaactgg aaagtggttt gcctttcagc acagcaatag
     7861 tagtccagat gtgatcacgc ttgccaaagg attgggttca ggtgtgccga ttggcgcatg
     7921 tattgcaaaa ggaccagcag cggagatttt caaaccaggt aatcatgcat caacttttgg
     7981 gggcaatcct ttagtatgta aagccgccct cacgacactt gaagttatcg agcaagatca
     8041 cctgatgcaa aatgctgtct caatgggaga cttgatgcgg aatcgttttc atgaaaagct
     8101 agcaaactgg tctaacgtca tcaagattcg tgggcaaggc ctaatgttag gtattgaact
     8161 gcctattgca tgcggagaat tagttaagga agcgctagct gcaaaattat tgattaacgt
     8221 tacttcagaa agagttgttc ggctactacc cgctttggtt attactcaag tcgaagctga
     8281 acagattgtg gataagacat catcgcttat tgatcgattt ctaacgacgc agtataacaa
     8341 tacgacgcct acgtaaataa agtaaatcga taacaacgac ctcatgactc ccaagcattt
     8401 cttgaagatt aatgatttta ccctcgatga atttgaatat ctattcgagc gcgcgcattg
     8461 gatcaagaat gaatttaaac aatatcgtaa gtattggcct ttaaccgaca gaacactggc
     8521 catgattttc gacaaacact ctacccgcac acgattatca tttgaagccg gcatgtgtca
     8581 gttaggtggc agcgtgattt atctttcaac gcgtgatacc cagttaggac gaggagaacc
     8641 cgttgaagac gcagcgcgcg tcatttcgcg tatggtcgat atcatcatga ttcgtacctt
     8701 tgagcatagc atcatcgaac gatttgcaaa tcattcccgt gttcccgtca ttaatggcct
     8761 tactgacgag tatcatccct gtcagattat tggggatatt tttactttta tcgaacatcg
     8821 cggaagcatc aaaggtaaaa cgattgcttg gatcggtgat tcgaataatg tttgtaatac
     8881 ctggctccaa gcggctgaag ttttcgactt taatgttcat gtttcaacac cgcccggcta
     8941 tgaagtcgaa cctgaacgag cagggttata cggcaccaat cactacgaag agtttagttc
     9001 tccccatgat gcggtgaaaa atgctgatct cgttacgacc gatgtttgga ccagtatggg
     9061 atttgaagat gagactgaaa tcagaaaaaa tgcattcgcg gatttctgcg ttgatgatga
     9121 aatgatggct tatgccaaag cagatgcgct gtttatgcat tgcttacccg cccatcgggg
     9181 tgaagaagta accaatgaag tcttagaggg gaggcaatcg gttgtttggg atgaggcaga
     9241 aaatcgatta catgtgcaaa aagcattaat ggaatatctg ctgcttggaa aaatttagca
     9301 acccgtgcgt tatcacggaa tcaattgaac tacgaaaata aaaaaagcga tgaaaaaagt
     9361 taaaaaagtg gtcttggctt tctccggcgg actcgatacg tcagttattt tgaaatggct
     9421 acaagaagtc tatcaatgcg aggtggtaac gtttacagca gatattggcc aaggtgaaga
     9481 aattgaaccc gcgcgtgcaa aagcccagcg gcttggcatt aaagaaatct ttatcgaaga
     9541 tttacgcgaa gaatttgttc gagaatatgt attcccaatg tttcgtgcaa ataccatcta
     9601 tgaaggtgaa tatctcttgg gtacgagcat tgcacgtcct ttgattgcca aaagacaaat
     9661 tgagatcgct cgagaaacag gggctgatgc ggtatcgcat ggtgcaactg gaaaagggaa
     9721 tgatcaagtt cgctttgagt taggttatta cgccctgcaa ccggatattc aagtcattgc
     9781 accgtggcga gaatgggatc tgacttcgcg tgagaaatta ttaagttacg ctgaacgcca
     9841 tggaatctct gtcgagatga agcaaaaagg gggttctcct tacagtatgg atgctaattt
     9901 actccatatt tcttatgaag ggcgatctct ggaaaacccg gcattagaac ctgaggagtc
     9961 aatgtggcgt tggacacaat cgccggaaaa agcacccaat gaatctgaat atatagaact
    10021 agaatatgaa cgaggagata tcgttgcaat taatggtaag cgcctaaccc cagcaaccat
    10081 tctcgaacaa ctcaatcaac tgggtggaaa acatgggatc ggtcgccttg atcttgtaga
    10141 aaaccgttat gtcggtatga aatcacgggg atgttatgaa acccctgggg gtaccatcat
    10201 gctccgtgcc catcgcgcca ttgagtctat tactttggat cgagaagtcg ctcatctgaa
    10261 agatgagctc atgccccgct atgcatcatt gatttacaat ggttactggt ggagtcctga
    10321 aagaatggca ttacaaacaa tgatcgatga atctcaagtc cacgtaaatg gatgggtacg
    10381 ggtaaaactc tataaaggaa acgttattat tgtaggccga gattctaaaa cagattcatt
    10441 gtttgatacc aatattgcaa cctttgaaga tgatgcaggc gcctataatc aagcggacgc
    10501 tgctggattt attcgcctca atgcactccg gttaagaatc gctagtaaaa ttagagcccc
    10561 cagaaaatag ggagtagggg aaccgaattc cgcttctttg attacattaa acttagcgat
    10621 tctgcgcaat gagttgatcg gttatataag gtgaaatgat ctccatcagt tgtggaaaaa
    10681 ttttagggtt gcccgccacg acctggccac tttttagata atgatcattc ccttccagat
    10741 cactcaccat cccacctgct tcgataatga gtaaacaccc agcagcgata tcccagggcg
    10801 acaaacccat ttcccagaaa ccatcgtatc gcccagcagc cacataagca agatcaagcg
    10861 ctgcagatcc tggccgccga atgcccgcgg tcttgggaat aaccgcttta aacatagcaa
    10921 gatacgcttc cataaaggaa aggtctcgga atggaaaacc agttcctatc aaactttctg
    10981 ataaattcgt tcgcttactg acacggatgc ggtggtcatt gaggtaggcg ccgcggccac
    11041 gactcgcagt aaaaagctcg ttgctaaccg gatcataaat aaccgcttgt gataagacac
    11101 cgcgatgtaa taacgcgatt gacacgcaat attttggaaa accatgcaaa aaattggttg
    11161 ttccatccaa cggatcgata atccattgat aatcagactg cttttgacct gactgactgc
    11221 cactttcttc tgctagaatg gcgtggttag gatacgcatc caataatatt tttatgattg
    11281 cctcttctgc agcaccatct acttcactga caaaatcact gtgtgatttt tgggtaacgt
    11341 tcagaatatc cagatttcta gaagcctgat taataatgtt gcctgcacgg cgcgcagctt
    11401 tcactgcaat ggtaagcatt ggatgcatgt taattgatca ataaattcaa caattcagta
    11461 ttctaatatg attcaccact ctccgcttga taatgtccga gttgttctca gtcacacgag
    11521 tcatcccggt aatattggag cttcggctcg cgctatgaaa accatgggtt taagctcact
    11581 ttatttagtc aatcctaaat catttcctga caatgaagct tatgttcgcg caaccaatgc
    11641 tcgagatatt ctcgatcaag cagtgatttg ctcaacgctt gatattgccc tgcaagactg
    11701 tgtattagca gttgcaatga cagcacgcgc tcgtgaatta acccatcagg taatcgatac
    11761 gaggcaaatc gctcaaaaac ttgttgaaat cgcaacggaa tcacccgtag cactggtttt
    11821 tggaagagaa agctctgggc taacgactgc tgaagtcacc aaatgccagc tcaccgtcca
    11881 tattccgacc agctcaacct atgcatcact taatcttgca gccgctgtgc aaatcatgtg
    11941 ctacgagtta cgtatgggcg cagttacacc agaaagcaca tcacctccac ctgaagtgcc
    12001 acaacttgcc agcttgaatg aaattgaact cttttatcag caccttgagg aagccctgat
    12061 tacaagcaaa tttctcgatc ctcagcaacc caaacgcctg atgcaacgaa ttcgtcgcct
    12121 attttctcga accgccttgg aaaaggagga agtcaatata ttgcgtggaa ttattcatgc
    12181 gttagcgaag gagaagcgat aactatatcg aatttacatg actggattga cttagtagtg
    12241 ttatttagcg atgattgcag atgatatttc tttattgacc ctacttctta tatttaagga
    12301 atgattatga caacgaatac gatctttaaa cataaagtta gcgcgattat taacaataag
    12361 cacttactta aacatccgtt ctatatggct tggaccgaag ggaaattaac ccaagaacag
    12421 cttcgccatt atgcagagca atatttctac aatgtattag ctgagcctac ctatctcagc
    12481 gccgttcact tcaatacccc tcatggtcat actgaggaaa actgcggtga tatcagcgta
    12541 cgtcaagaag tactcaaaaa cttgattgat gaagaacatg gttcaggtaa tcatcctgct
    12601 ctgtggaaag cgtttgcatt tgctttaggt gcgaatgata aaagtctagc aaatgccgca
    12661 gcactgccag aaaccgataa cttggtgtca actttccgtg atatttgctt gaatagcccg
    12721 ttctatgttg gattggctgc tttacacgca tttgaatcac aagttcctga tattgctgca
    12781 gtcaaaattg aaggtttggc taaattctat ggaatgaata atccgaaaga ttatgaattc
    12841 ttctcagttc atcaaaaagc cgacatttat cattctgagg cagaatggtc aatcatcgag
    12901 cggtttgcag atacgcctga aaaacaagca gcagttttag ccgctacaca gcgcgcttgt
    12961 gacgctttat ggaaattctt agatggtatc catgaaaatt attgcgctaa cttgatttgt
    13021 gatcagcaaa cagcggttac gcttcattaa ttaacgcggt ggatagatga gttttatctc
    13081 atccatccac ccgcttattg caatattcaa ttagaaaact gcactgaagc caacttgact
    13141 actaaacccc gcttctggcg caaagctgcg taaaaacgaa gtggaattac gaatcgtcgc
    13201 attggtcatg ttagacagct tcgcaaagaa gagaatcttc atacgatctg atgctttcca
    13261 atcataagtg atatcaagat ctaatcggtt atagcccggt gtctcagtct ctagaagacc
    13321 tggtctattt tgctccaacg catgcgtata gcgcaaaagg cttctccatt gcatccattg
    13381 caatccaaac tcagcaccaa accgcaatgg tgggagtctt ggaacatctc ccaatgatcc
    13441 actttggaat gaacctcgta cataatctga aaagaatgtg aagcgcggat tataagtagc
    13501 agctatttta gggcgcatgg taatttcagc ttcatagccc cagaaatttg cagtatcagc
    13561 ctgatagcca aaaatgggta ggcaatttct taaatttgca caagctaatt ggaaacgcgg
    13621 gggatcgggc gcaaagttaa aaaagaaacc ttgatttttt aaataaataa aatcttcaac
    13681 agatttatag taaccattcg cattaaaaga aatgacatta ccagcgtaac gtagccctaa
    13741 ttcgtaatga tctgtttgtt cagtatttaa atccatgcgc cctaattgat agctacgcgt
    13801 agcaaaatta ggacccgatg caaatagctc ttgaacatca gggctacgtt gcgcacgttg
    13861 ccagtttaga aaaaatgacg tcagtggcaa tatttcgtaa ttcaagctag ccgccagcga
    13921 ataggtcaaa taattgagcg aattaggcag cggcaacggc gaggaaatac cggcaagccg
    13981 cagctcattg cttttaggcg aggtggtttg gtgctctatt cgccccccta attcaaatgt
    14041 cgttcgatca ttaacgacta gtgtctcggt tagaaaaaaa ccgattgaat ctacattcga
    14101 tgatggggta aacgtttcta gacctgtcgc agaaaaatta cggttctgcc attgcatccc
    14161 aaatttacca tccaaccaat ctcccttctg gtgattcatc tccagacgag attcccaaac
    14221 agaattacca aatcgggtta gtgtagagtt tcttgctgat atatttttat caaagtcttt
    14281 atgatcgtaa tcgatatagc tgagcttggc tttgatcgat ggcaatgctc cccagtaggt
    14341 tttccagaaa ccttctaaat cgtagcgtcg ctgattcata tcaatacgaa ttgcttcttg
    14401 ttcggatcga ctactgtcac cgtgaccagg aacacctgga gggataccat agtttcttgc
    14461 agtttcatga tagctcccac ccaaataacc atattttcct accaaagaaa ttccagcaga
    14521 gccgccctgg ctatgtgaat ggctattttg aatcacatta ttactattcg tagtaggttc
    14581 tacctgaaat tgctgccgaa tcgccgattc atccaatgct gagccgggaa tgtgcatgtt
    14641 attggctgat cgatggaagt aatcaaaatg caaggcaacc atgctgcgcc caatatccaa
    14701 acttatcatt cccgcctttt cacccggatt atggtcaaaa cgaaaatcag ttctaccgga
    14761 catgccattc gttggaattc gatcaggaat acgctggtga ctgatatcga cgacaccccc
    14821 gattgcgttt gctccatact gcagggttgc tgggcctttt ataaccgtta ttccat
//
LOCUS       DAOQKQ010000053        14316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_1977_length_14316_cov_6.510062, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000053 DAOQKQ010000000
VERSION     DAOQKQ010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 14316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14316
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1977_length_14316_cov_6.510062"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            224..2131
                     /gene="thrS"
                     /locus_tag="PKZ49_04990"
     CDS             224..2131
                     /gene="thrS"
                     /locus_tag="PKZ49_04990"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HND35954.1"
                     /translation="MVAIRLADGSERRFDQSITALDVAESISPGLARSAIAAKVNGKL
                     ADLSYLIEEDSELILITEKDSEGLDIIRHSSAHLLAHAVKELFPEAQVTIGPVIEDGF
                     YYDFAYKRPFTTEDLATIEKRMMEISRRNLVICRKEAERSAAILFFKNQGEHYKAQLI
                     ESIPGNETISLYSQGEFTDLCRGPHVPSTNKLKVFKLMKVAGAYWRGDSKNEMLQRIY
                     GTAWLKKEDQEAYLKRLEEADKRDHRKLGKQLDLFHIQDEAPGMVFWHPKGWALWQQI
                     EQYMRQVLNRSGYQEIRTPQVLDRSLWEHSGHWENFRENMFVTESEERHFAIKPMNCP
                     GHIQIFNQGLKSYRELPLRLAEFGSCHRNEPSGALHGIMRVRAFTQDDAHIFCTEEQV
                     QSEVIQFIDLLKQVYKDFGFDAILVKLSTRPQKRVGSEQQWDDAESALESALEAKKLD
                     WQIQPGEGAFYGPKIEFSLRDSLGRVWQCGTLQLDFSMPARLGATYVSEDNLKKTPVM
                     LHRAILGSLERFIGILIENYAGALPVWLAPVQVAVLNISKNQMEYAQEIEKALSSNGI
                     RVLLDLRNEKISYKIREHSLQKIPYQIIVGDEEVNGNIIAVRNRLNKDMGQMTVANFI
                     ECIHADCMKKL"
     gene            2153..2740
                     /gene="infC"
                     /locus_tag="PKZ49_04995"
     CDS             2153..2740
                     /gene="infC"
                     /locus_tag="PKZ49_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HND35955.1"
                     /translation="MIQERSVRINEEINAPVVRLIGLKGEQLGVVSLTDANARAEEES
                     VDLVEIAPGADPPVCRLMDYGKYLYQESKKKHDAKLKQKQVQVKEIKFRPNTDEGDYN
                     IKLRSIINFLDDGDKVKITLRFRGREMAHQEFGVRLLDRIKEDLQTYGLVEQHPKLEG
                     RQMVMVLSPKKKEVKSAKSKESTKEKDKPETIISS"
     gene            2881..3078
                     /gene="rpmI"
                     /locus_tag="PKZ49_05000"
     CDS             2881..3078
                     /gene="rpmI"
                     /locus_tag="PKZ49_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HND35956.1"
                     /translation="MPKMKTKKSAAKRFLVRASGSIKRSQAFKRHILTKKTTKNKRQL
                     RGTTHIHSSNIASVNAMLPYL"
     gene            3091..3450
                     /gene="rplT"
                     /locus_tag="PKZ49_05005"
     CDS             3091..3450
                     /gene="rplT"
                     /locus_tag="PKZ49_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HND35957.1"
                     /translation="MPRVKRGVTAHARHKKILKLAKGYRGRRKNVFRIAKEAVMKAGQ
                     YAYRDRRQKKRQFRVLWIARINAAVREHGLSYSNFMNGIKKAGIELDRKVLSHLAVFD
                     KAAFEEIVKKARTSLAV"
     gene            3553..4572
                     /gene="pheS"
                     /locus_tag="PKZ49_05010"
     CDS             3553..4572
                     /gene="pheS"
                     /locus_tag="PKZ49_05010"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HND35958.1"
                     /translation="MVEIDGLVNEAIALLNKAGTIIELDQVKVRYLGKEGELTKLLKG
                     LSKLSQDERPAAGNRINQGKKQVEEALNQRRAILLENEVQIRLAADNIDVTLPGRGLS
                     RGGLHPVSIVLERIERLFRSIGFSVASGPEIETDFYNFTALNIPQNHPARAMHDTFYV
                     ENGDLLRTHTSPVQIRFMAEYKPPLKVIAPGRVYRCDSDVTHTPMFHQVEGFWVDEEV
                     NFSALKGILMTFMQNFFEREDLKVRFRPSFFPFTEPSAEMDISCVMCNGSGCRVCSHT
                     GWLEVLGCGMVHPNVLKYVNIDSEKYTAFAFGVGVERLTMLRYGVSDLRLFFENDLRF
                     LAQFN"
     gene            4594..6981
                     /gene="pheT"
                     /locus_tag="PKZ49_05015"
     CDS             4594..6981
                     /gene="pheT"
                     /locus_tag="PKZ49_05015"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HND35959.1"
                     /translation="MRFSENWLRSLVNPPYSRDQLVHTLTMAGVEVESVDEVASAFDN
                     VVVAEVISVQKHPDADRLTICNVDVGLANNTLLQIVCGAANVSESIKVPCALVGACLP
                     GGIVIKQSKLRGVESFGMLCSAQELGLGEKKEGLLVLPGDAPVGMNFRDYYQLEDRIF
                     TLKLTPNRADCLSLNGIAREVAAITDTPLHVPEYELARDSCVDRLTVNVEVPDACPLY
                     CGRIIRNVTPATPTPLWMVHRLERSGIRAINIVVDILNYVMLEMGQPMHGFDLAKISD
                     QLLGKVVVRFAHSDETLKLINGENLELKSDMLVIADTNRAVALAGIMGGLETSIQNET
                     TDVFLESAYFSPHVISGKSFKLGFNTDSAYRFERGVDFASTRKALERATQLILELMDD
                     AVAGPITQVESSLPQRSPVMVRLSKIQQVLGINLNSNQIASILERLRFSFSEDKGIFH
                     ISPPSYRFDLAIEVDFIEEVARIYGYDRIPISLPKTTVSMLPDSELGRLSPSQLRNML
                     VLRDYQEVINYAFVDPQWELDFSKDCIPIALKNPIASQMSVMRTTLIGGLIANLQFNI
                     SRKQSRVRLFEIGCCFLRGAGDNYQQIEKIAGICYGDTLEEQWGVTSRNVDYFDIKMD
                     IESVYLPQKVTFNKCEHHALHPGKSAQIIIDNHVVGWLGELHPRLQTQYDLPRPVILF
                     ELSLDSLDRKHLSKAKVLSKFPPVYRDIAIVISEHVTNDQLLKTMKDEQLHIVKDIYL
                     FDYYSGKSLEQGKKSYAYRILLQDNEKTLTDQEVEVAILKLISVLQAKYDAKLRV"
     gene            7255..7569
                     /locus_tag="PKZ49_05020"
     CDS             7255..7569
                     /locus_tag="PKZ49_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HND35960.1"
                     /translation="MALTKAELTDLLFESVGLNKREAKEVVESFYEELSLALQKGEGV
                     KLSGFGNFQLRTKPCRPGRNPKTGEEIPISARRVVTFHASQKLKSMVEFNYHRKNGAR
                     QP"
     gene            7550..7930
                     /locus_tag="PKZ49_05025"
     CDS             7550..7930
                     /locus_tag="PKZ49_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HND35961.1"
                     /translation="MERDSRSYPPIPAKRYFTIGEVSELCGVKPHVLRYWEQEFIQLR
                     SIRRRGNRRYYQHHEVLLIRRIHELLYGQGFSISGARTRLSNDGGLESGLIEDPSQTI
                     TACSTEELLYIRKEIKAIISLLRS"
     gene            7988..8064
                     /locus_tag="PKZ49_05030"
     tRNA            7988..8064
                     /locus_tag="PKZ49_05030"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8022..8024,aa:Pro,seq:ggg)
     gene            8107..8856
                     /gene="surE"
                     /locus_tag="PKZ49_05035"
     CDS             8107..8856
                     /gene="surE"
                     /locus_tag="PKZ49_05035"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HND35962.1"
                     /translation="MRILLSNDDGYFAPGLAHLANALSTIAEIVVVAPERDRSGSSNS
                     LTLDRPLKLHKSPNGFYFVNGTPTDCVHLAVTGMLDYLPDMIVSGINDGANMGDDTIY
                     SGTVAAATEGFLFGIPSIAVSLVNRSECNFATAAQVALEMVKRFMEKRVHVPVLLNIN
                     VPDVPLSRLAGMEVTRLGRRHKAEPVIKSTNPRGETIYWVGAAGIAQDAGEGTDFFAV
                     QNNKISITPLQIDLTRYDQIGYLKDWLDLSD"
     gene            8925..9560
                     /locus_tag="PKZ49_05040"
     CDS             8925..9560
                     /locus_tag="PKZ49_05040"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HND35963.1"
                     /translation="MTSQRTRARMVERLREQGITDEMVLAVMNTIPRHLFVEEALASR
                     AYEDVSLPINFGQTISSPWIVARMTELLRGNHNLNKVLEIGTGCGYQTAVLAKLAKEV
                     YSIERIGPLLTRTRIRLRELRITNISLKHADGLLGLPDVGPFDAIIMTAVTTQIPELL
                     LEQLTVGGRMVYPKGISKQFLCVIEHTSQGFIETILDEVKFVPILSGVVNR"
     gene            9587..10678
                     /locus_tag="PKZ49_05045"
     CDS             9587..10678
                     /locus_tag="PKZ49_05045"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HND35964.1"
                     /translation="MFSQLIKERIVFGASTKMCFGLNVNRYILVTDIYQVLLLLLMCW
                     VVTGCVSPNTAPIVESQQNPTRVPSPEVVKPRIQNKQLYVVQRGDTLYGIALKHGVDY
                     KELVQWNGIADPNTLKVGQEIDLLVPSRQSKVAEIAPEDQSAQPTLFALPQSAAPVEV
                     NSIKPLQTEPVSPSPGAMVIAEPKSIKLPYAEPSAAKPDLEVSQSTKNQPAAILPPPN
                     KIEKPSDVEKPTKAQPEIALPKNNNNSIKWSWPTNGKVLSKYTERTKGVSISGNKGQS
                     VLASADGTVVYSGNSLRGYGNLIIVKHNDIYLSAYGHNSKILVREGETVSKGQKIAEM
                     GNTDSETVKLHFEIRERGKPVDPLKFLSN"
     gene            complement(10741..11259)
                     /locus_tag="PKZ49_05050"
     CDS             complement(10741..11259)
                     /locus_tag="PKZ49_05050"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HND35965.1"
                     /translation="MTYIFSFEPIADHHASVLILGSMPGNASLMAGQYYAHKQNVFWK
                     IMAELLQFELNSSYETRVQKLKSAHIALWDVLRSCKRVGSLDSNILPNSQTVNNLQHF
                     LQQHHLISKVCFNGSKAENCFNRYVAKTISGSPKQFIRLPSTSPANASIPYEKKLKIW
                     QEALFPSLRSNL"
     gene            complement(11323..13215)
                     /locus_tag="PKZ49_05055"
     CDS             complement(11323..13215)
                     /locus_tag="PKZ49_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HND35966.1"
                     /translation="MVASKFQLRPLIAFIHDFLAIIIAWWLAYLFRFNFEIPPHFQAS
                     LVENLLWIVPLQASFFYLFRLYRGIWRYASIPDLKRIFWAVTLSAMVVPFVLFMLKLP
                     GVVPRAVIVLAPLLLMLIMGGSRILYRSWKEQRLYSFKNSERNTVIILGTGSMAVNLV
                     KELAHSPDWQVVGLMGDSPKKQGSRLHGVRVLGKINELDYWVEKLSVRHIIIAMPPTQ
                     TNERRQALEICSEVGVKAMTIPSYTDLVSGKMTVSQIRDIELDDLLGREPVILDTEGL
                     HTLLSNKTILITGAGGSIGSELCRQIITFKPKQLVLFELNEFALYSIQEEFCSKFPDI
                     AMAFVIGDVKDESRLAQLFAQYQPTVIFHAAAYKHVPLMEKENTWQALQNNVLGTYVL
                     AKLAIKHHITKFVLISTDKAVNPTNVMGASKRLAEMVCQALQQSILSQESMQANTIYS
                     PSTRFVIVRFGNVLGSTGSVIPKFREQIARGGPITVTHPDISRYFMSIQEAAQLVLQA
                     GSMGGIKGGGEIFVMDMGNPIRIVDLAKDMIRLSGFSEEEIKITYTGLRSGEKLHEEL
                     ITENESTLPTQHEKLRITLSHPVDERWLTSFITWLIEQPALSDTEVRMALAKWVPEYS
                     DKNNEH"
     gene            complement(13221..14210)
                     /locus_tag="PKZ49_05060"
     CDS             complement(13221..14210)
                     /locus_tag="PKZ49_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HND35967.1"
                     /translation="MILGLPFQLFVAPILTFLIAYLTIFWLIRLGNSLNIIDIPNDRS
                     LHAKPIPRTGGLGLIGAIFITGLLFVDIFSVPVFIGLAFVSAISFADDIKPLPVWLRL
                     SIHTIAAMLLVVNLFLDTYSWLFVITVGLGIIWITNLYNFMDGSDGLAGGMTAIGFGY
                     YGIAATLAGDIDFATINFSIAASALAFLRFNFYPAKIFMGDVGAISLGFLAGALGLLG
                     WKNNLWSLWLPLLIFSPFIADATVTFIKRLLRGKKIWQAHNEHYYQRLIKGGLGHRNT
                     ALLAYILMLVVGGTAILSAKFEFAQQSWISSMWGIAYLVIMHAFDRHQSHHNR"
BASE COUNT         4458 a         2488 c         3212 g         4158 t
ORIGIN      
        1 gaagctaaat ctgaagcttc cgatgatgat gaagatgacg aggatgagta aggttttaaa
       61 cgagacctgc gtggcattca tcatttgatt atttagattt ttgtttggct gatgtaaccc
      121 gcgtaaattt aatttacgcg ggtttttctt tatttgaata gacaaaatac tgtaatcttt
      181 tatttttggt atagagttaa cattttattt aggaaggatc attgtggttg ccatacgatt
      241 agctgatggt tcagaacgta ggtttgatca aagtataact gcgttagatg ttgctgaatc
      301 gataagtcct ggacttgcac gttctgcaat tgcagcgaaa gttaatggga aattagctga
      361 tttatcctat ttaatagaag aagatagcga gctaattctg attactgaga aagattcaga
      421 ggggcttgat attattcgac actcttctgc tcatctgctt gcgcatgcag ttaaggaatt
      481 atttccagaa gcacaagtaa caatagggcc tgtcattgaa gatggttttt actatgattt
      541 tgcgtataag cgcccattta ctacagaaga cctcgctacg atcgaaaaga ggatgatgga
      601 gattagtcga agaaacttgg taatctgtag gaaagaagcg gaacgttctg ctgcaattct
      661 tttctttaaa aatcaaggag agcattataa agcacagttg atcgagtcga ttccaggcaa
      721 tgaaacgatt tcactttatt cacaaggtga atttactgat ttgtgtcgag gaccgcatgt
      781 gccaagtacc aataagttga aagtgtttaa acttatgaag gtggctggtg cttattggcg
      841 cggggattct aaaaatgaga tgctacaaag aatttatggc acggcttggt taaaaaagga
      901 agaccaggaa gcttatttaa agcgacttga ggaagccgac aaaagagatc accgaaaatt
      961 aggtaagcaa ttagatttgt ttcatattca agatgaggcg cctggtatgg ttttctggca
     1021 tcccaaagga tgggcgcttt ggcaacaaat cgagcaatat atgcgtcaag tattgaaccg
     1081 aagtggatac caggaaattc gaacgccgca agtattagac cgatctttgt gggaacattc
     1141 tggtcactgg gaaaattttc gggaaaatat gtttgtgaca gaatcggagg aacgccattt
     1201 cgccattaaa ccgatgaatt gtccaggcca tatacaaata tttaatcagg gtcttaaaag
     1261 ttatcgagaa ttacctttgc gacttgctga atttggctca tgccatcgca atgaaccgtc
     1321 aggagcattg catggaatta tgcgtgtaag agcatttaca caggatgatg ctcatatttt
     1381 ttgtacagaa gagcaagttc agagtgaagt tatacaattt atcgatttat tgaaacaagt
     1441 ctataaagat tttggatttg atgcgatctt agtaaaactt tctactcgac cacagaaaag
     1501 ggtgggttca gaacagcaat gggatgatgc cgaatcagcc ttggaatcgg ccttagaagc
     1561 taaaaaacta gattggcaaa tacaaccagg ggaaggtgca ttttatggtc ccaaaattga
     1621 attttcttta agagacagcc tcgggcgagt gtggcagtgt ggaactttgc aattagattt
     1681 ttcaatgcca gctcgactgg gcgcaactta tgttagtgaa gataatttaa agaaaactcc
     1741 agtaatgtta caccgcgcaa ttttaggttc gttagaacga tttattggta ttcttatcga
     1801 aaattacgca ggcgcactac ctgtgtggct tgctccagtt caagttgcgg ttctaaatat
     1861 ttcaaaaaat cagatggagt atgctcaaga aattgagaaa gcactcagtt caaatggaat
     1921 tagggtatta ttagacttga gaaatgagaa gataagctat aaaatccgcg agcacagttt
     1981 gcaaaaaata ccttaccaga ttatcgtcgg tgatgaggaa gtaaatggta atattattgc
     2041 tgtaagaaat cgtttgaata aagacatggg acagatgacc gtggctaatt ttatagaatg
     2101 tattcatgct gattgtatga aaaaattata agttttataa ggagaagtaa ctataattca
     2161 agaaagaagc gtaaggatca atgaggaaat aaatgctcca gtggtaagat taatcgggtt
     2221 aaaaggggag cagttaggag tagtgtcact tactgatgca aatgcacggg cagaagaaga
     2281 aagtgtcgat ttggttgaga ttgcaccagg cgctgatcct ccagtttgtc ggttaatgga
     2341 ttatggaaaa tatctctatc aagaaagtaa aaagaagcat gacgcaaaac taaaacaaaa
     2401 acaggttcaa gttaaagaga taaaatttag acctaataca gatgagggtg attacaatat
     2461 aaaactgcgc agtattatta acttcttgga cgatggagat aaagttaaga ttacgctacg
     2521 ttttagagga agagaaatgg ctcaccagga atttggtgtg cggctcttgg atagaattaa
     2581 agaagatctc caaacttatg gtttagtgga acaacatcca aagttagaag gtcgacaaat
     2641 ggtaatggta ttatcaccta agaaaaaaga ggtgaagagc gcaaagtcta aagaatcgac
     2701 aaaagagaaa gataagccag agacaattat ttcatcttaa gtgtaaaact cctctctacc
     2761 gcccaatagc ggtaggccag aattgagcat agttgagggc tagcattaag attcagaatt
     2821 cacgtattgt gttatagatt tcagtaaata aagattttag aaattaggag aaaatttatt
     2881 gtgccaaaaa tgaaaactaa aaaaagtgct gctaagcgct ttcttgttag ggcaagcgga
     2941 agtattaagc gatcacaagc atttaagcga cacatcttaa cgaaaaagac tactaaaaat
     3001 aaaagacagt taagaggaac cacacatatt cattcaagta acatagcttc tgttaatgct
     3061 atgttacctt atttgtaagt cgggggagca atgccaagag taaagcgtgg agtgactgcg
     3121 catgcacgtc ataaaaaaat tcttaagtta gcaaaaggtt atcgcggtcg ccgtaagaat
     3181 gtatttagaa tcgccaaaga agcggtgatg aaagcaggtc agtatgctta tcgggatagg
     3241 cgacaaaaga aaagacagtt ccgagtttta tggatagcaa ggatcaatgc agctgttaga
     3301 gagcatggac tatcctatag taattttatg aatggaatta aaaaggctgg tatagaactt
     3361 gataggaagg tcctatccca tcttgccgtg tttgataaag cagcttttga ggaaatcgtc
     3421 aagaaagcta gaactagcct agcggtgtaa ttagtttatt ggattggtat tgtttgtaag
     3481 ggagatcaag gagattttga cttgatctcc ctttttgttt tcatcgtttt tttattataa
     3541 ataatacgat ttatggttga aattgatggt cttgtgaacg aggcaattgc gctattaaac
     3601 aaggcgggca cgataattga gctcgatcag gtcaaggttc gctatcttgg aaaagaaggc
     3661 gagttgacga aattgcttaa agggttgagc aaactttcgc aggatgaacg tcccgcagcg
     3721 ggtaatcgaa ttaatcaggg gaaaaaacaa gtagaagaag cgctcaatca gcgaagggca
     3781 atactattag aaaatgaggt acagatacga ttagcagcag acaatattga tgttactttg
     3841 cctggtcgag gattgtcgag aggtggattg catcctgtct cgattgtgct ggaacgtatt
     3901 gagcgactgt ttcgatcgat tggcttctct gtcgcatctg ggccagagat tgagaccgat
     3961 ttttataatt ttaccgcact aaatattcca cagaatcatc cagcaagagc gatgcacgac
     4021 acattttatg tagaaaatgg tgatctgttg cggacgcata catcaccggt tcaaatccgt
     4081 tttatggccg agtataaacc tccattgaag gtaattgcac ctggtcgtgt ttatcgatgc
     4141 gattcagatg ttactcatac tccgatgttt caccaggttg aaggattttg ggtggatgaa
     4201 gaagtgaatt tctctgcttt aaagggaatc ttaatgactt ttatgcagaa tttctttgag
     4261 cgtgaagatt tgaaagtacg ttttagacct tcttttttcc catttactga accctctgca
     4321 gagatggata ttagctgtgt catgtgcaat ggttctggtt gcagagtttg tagccatacc
     4381 ggctggttgg aagtattagg ctgtggaatg gttcatccaa atgtactcaa gtatgtcaat
     4441 attgatagcg agaaatatac agcttttgcg tttggggtag gcgtggaaag attaacgatg
     4501 ctgcgctatg gagtcagtga tttacggttg ttctttgaaa atgatttacg tttcttagcg
     4561 caatttaatt aaattttgta ggtttctctc atcatgagat tttcagaaaa ttggctgcga
     4621 agccttgtta atccccctta ttctagagat caattagttc atacacttac catggcaggt
     4681 gttgaagtgg aatctgtgga tgaggttgcg tctgcatttg ataatgtagt tgtagctgaa
     4741 gttatttcgg ttcaaaaaca tcccgatgca gaccgactaa caatttgtaa tgttgatgta
     4801 gggctagcca acaatacatt acttcaaatc gtttgtggtg cagcaaatgt atctgaaagt
     4861 atcaaggttc cctgtgcttt ggtgggcgca tgtctgcctg gtggaatcgt catcaagcaa
     4921 agcaaattaa ggggagtgga gtcatttggg atgctgtgtt cggcgcaaga actaggactg
     4981 ggtgaaaaaa aggaaggatt actcgttttg ccaggggatg cgcctgtagg catgaatttt
     5041 cgcgactatt atcagcttga ggatcgtatc tttacgctta aattaacgcc taatcgtgct
     5101 gattgcctaa gtctaaatgg catcgcacgc gaggtggctg cgatcaccga tacgccattg
     5161 catgttcctg agtatgaatt ggctagggat agttgtgtag atcgtttgac ggtcaatgtg
     5221 gaagtgcctg atgcctgccc gctatattgt ggtcgtatta ttcgaaatgt tacgccagcg
     5281 actcctactc ctttatggat ggttcatcgg ttagaacgaa gtgggattcg tgctatcaat
     5341 atcgtggtgg acattcttaa ttatgtgatg ttagaaatgg gtcagccgat gcatggtttt
     5401 gatttagcta aaatttcaga ccaattattg ggaaaagtag ttgttcgttt tgcgcattcc
     5461 gatgaaacac tcaaacttat taatggagag aatcttgagc tgaaatcgga tatgctggta
     5521 attgcagata ccaaccgggc agttgcatta gctggaataa tgggtggact ggaaacgagt
     5581 attcagaatg aaactactga tgtatttcta gaaagtgcct atttttctcc tcacgtaatc
     5641 agtggaaaat cattcaaact aggatttaat accgattcag cataccgctt tgaacgagga
     5701 gttgattttg catcaacccg aaaagccttg gaaagggcaa ctcaactaat tttagaatta
     5761 atggatgatg cagtagcggg gccaataacg caagttgaaa gctcattacc acaacgaagt
     5821 ccggttatgg tgcgactttc aaaaatacag caagtcttag gaatcaattt aaatagcaat
     5881 cagattgcca gtattttaga acgattgaga ttcagcttta gtgaagataa gggcattttt
     5941 catatatcgc ccccgtctta tcgttttgat ttagcgattg aagtggattt tatcgaggaa
     6001 gtcgctcgta tatacggata tgatcgtatt ccaatcagct taccaaagac gacagtgtcg
     6061 atgcttcctg attcagaatt gggaaggctc tcaccctctc agcttaggaa tatgctggtt
     6121 ttgcgtgatt atcaggaagt tattaactat gcatttgtag atcctcaatg ggagctcgat
     6181 ttttctaaag attgcatccc tattgcttta aagaacccta ttgccagtca aatgagtgtc
     6241 atgcggacta cattgatagg agggctaatt gccaacttgc agttcaatat cagtcgcaag
     6301 caatctagag tgagattatt tgaaattgga tgctgttttt tacgtggtgc aggagataat
     6361 tatcaacaaa tcgaaaaaat tgctggaatt tgctatggag ataccctcga agaacagtgg
     6421 ggagtgacat cgcgaaatgt agattatttt gatattaaaa tggatatcga atcagtgtat
     6481 ttgccacaaa aggtaacttt taataagtgt gagcatcatg cactacatcc tggaaaatca
     6541 gcgcagatta tcattgataa ccacgtagtg gggtggcttg gagagttaca tccacgctta
     6601 caaactcaat atgatttacc tcggcctgta attttattcg agttgagttt ggattctctg
     6661 gatagaaagc atttatctaa ggctaaggtt ctttctaaat ttccacctgt ttatcgtgat
     6721 attgcgattg ttatctctga acatgtgacg aatgatcaat tacttaaaac aatgaaagat
     6781 gagcaacttc atattgtcaa agatatttat ctttttgatt attacagcgg aaaatcgcta
     6841 gagcaaggta agaaaagtta tgcatatcgg attttattgc aagataacga gaaaacctta
     6901 actgatcaag aagtggaagt ggcaatctta aaactaatca gtgtgctgca agctaaatat
     6961 gatgcaaagc ttagagtgta atttcttcgc ttcgatagta aaatattctc tatcgtattt
     7021 tgattgatta aaagtgctgt tgagttaaga atgagtttag gctatcaatc gcaagtaaca
     7081 taaaatcctt gttcaagttt tcgaactgaa tctctgcata taaaaaaaga gtcaaattct
     7141 aatagcaata aagcagtcta tcgaagcgtt gttttgtttc taaaaaccta aaactttggg
     7201 taaatgctgg atataggttg tgtattcgtg tgatttaaat ttaatgaggg agatatggca
     7261 ttaacaaaag cggaattaac tgatttgttg tttgaaagtg taggacttaa taaacgcgag
     7321 gcaaaagaag tcgttgaatc tttttatgag gaattaagtc ttgcccttca gaaaggagag
     7381 ggagttaagt tatcaggatt tggtaatttt cagttgcgta ctaaaccctg tcggcccggt
     7441 agaaatccta aaacaggtga agagattcct atttcagcga ggcgagtcgt tactttccat
     7501 gcaagtcaaa aattaaaatc gatggtagag tttaactatc atagaaaaaa tggagcgaga
     7561 cagccgtagt tatcctccta tcccagcaaa gcgctatttc acaattggag aagtgagtga
     7621 attgtgtggt gttaagcccc atgttctgcg ctattgggag caagaattta ttcaactcag
     7681 atcaattaga aggcgtggta acagacgtta ctatcaacat catgaagtgt tgctaattcg
     7741 tcgcattcat gaattgttat atggacaagg tttttctatt agtggagcaa gaacccgttt
     7801 atctaatgat ggtggtcttg aatcaggttt gattgaagac ccgtctcaaa caatcacagc
     7861 atgttcaact gaagaactgt tgtatatccg caaagaaatt aaggcgatca tctctttatt
     7921 gcggtcttaa gcgatattca atcgctaatc taaaactttg tttgctataa tttggaaaga
     7981 gaaaagacgg ggcgtagcgc agcctggtag cgtacttgca tggggtgcaa gtggtcggag
     8041 gttcaaatcc tctcgccccg accaaggcgt tgtataatat tattatcata atgaattaaa
     8101 tctcaaatgc gcatattgct tagtaacgat gatggatact ttgcacccgg tcttgcgcat
     8161 cttgccaatg ctctctcgac aattgctgaa attgtagttg ttgcacctga aagagatcgt
     8221 agtggttcaa gtaactcttt aacgcttgat cgcccactta aattgcataa atcacccaac
     8281 ggtttttatt ttgtgaacgg tacaccaacc gattgcgtcc atttggctgt aacgggaatg
     8341 ctcgattact taccggatat gatagtgtcg gggatcaatg atggcgctaa tatgggtgat
     8401 gatacaattt actccggaac agttgccgca gcgactgaag ggttcttgtt cggaatacct
     8461 tccatcgctg tgtcattagt aaatcgatct gagtgtaatt ttgctactgc tgcccaagtt
     8521 gcattagaaa tggtcaagcg gtttatggag aaaagagttc atgtgcctgt tttattaaat
     8581 atcaatgttc ctgatgtacc attgagtcga ttagccggca tggaggttac tcgtttaggc
     8641 cgtcgccata aagcagagcc agttattaaa tctacaaacc cgagagggga aaccatttat
     8701 tgggttggag cagctggtat tgcacaggat gcgggtgaag ggactgattt ttttgcagtt
     8761 caaaataata aaatatcaat tactcccttg caaattgacc ttacacgata tgaccagata
     8821 ggctatttaa aagattggtt agatttatcg gattgagtga gtcttgttga aataaattga
     8881 ttgaaaaggg gtagagagtg gatgctcatc atcatggcat aggcatgact tcacagcgta
     8941 ctcgagcgag aatggttgaa cgattacgtg agcaaggtat tacagatgaa atggtacttg
     9001 cagtaatgaa cacgattcct cgccatcttt ttgtagaaga agcgttggca tcaagagctt
     9061 atgaagatgt ttcgttaccg attaattttg gacaaacgat ttcgagccct tggattgttg
     9121 ctagaatgac tgagctgctc cgaggaaatc ataatctaaa taaagtgctt gaaattggaa
     9181 cagggtgtgg ttaccaaacg gctgtattag cgaagttagc caaggaagta tattcgatag
     9241 agcgtatagg cccactgctg acgagaacgc gtatccgatt gcgagagcta agaataacca
     9301 atattagcct taaacatgca gatggattgt taggtttgcc tgatgtaggg ccttttgatg
     9361 caattattat gacagcagta acgactcaaa tacctgaatt actattagaa caattaactg
     9421 tgggggggag gatggtttac ccaaaaggta tttcaaaaca attcttgtgc gtaattgaac
     9481 atacgtccca aggttttatt gaaaccatac ttgatgaagt aaagtttgtg ccaattttat
     9541 ctggtgtagt aaatagatag atgatagtag gggagggtta tctgggatgt tcagtcaatt
     9601 gataaaggaa agaatcgtgt tcggtgcatc aacaaaaatg tgttttggcc taaatgttaa
     9661 ccgatatatt ctggttactg atatctatca agtcttatta ctgttactga tgtgttgggt
     9721 cgttacaggc tgtgtatcac ctaacactgc accgatcgtt gaatctcagc aaaatccaac
     9781 acgtgtacca tctcctgaag tggttaaacc gagaatacag aataagcagc tctacgtggt
     9841 tcagagaggg gatacgttat acggaattgc actgaaacat ggcgttgatt ataaggaatt
     9901 agttcaatgg aatggaattg ccgaccctaa tacattgaaa gttggtcaag aaattgactt
     9961 gctagtccct tcacgccaat caaaagttgc tgaaatagcg ccagaagatc aatctgcgca
    10021 accaacatta tttgcgttgc cgcagtcggc agctccagta gaggtcaatt caattaagcc
    10081 actacagaca gaacctgtta gtccgtcacc tggagcaatg gtcattgcag agcctaagag
    10141 tattaagcta ccttacgctg aaccatctgc ggctaaacca gatttagaag taagtcaatc
    10201 tactaagaat cagcctgctg ctattttgcc tccgcctaac aaaatagaga aaccaagtga
    10261 tgtagagaag cctacaaaag ctcaaccaga aattgcgcta cccaaaaata ataacaacag
    10321 tatcaagtgg agctggccaa ccaatggaaa agttttgagc aaatatactg aaagaacaaa
    10381 aggggttagt atttctggga ataaaggaca atcggtattg gcctcggcgg atggcactgt
    10441 tgtttatagt ggaaatagtt tacgcggcta tggaaattta atcattgtga agcacaatga
    10501 tatttattta agtgcatatg ggcataacag caaaattttg gtccgtgaag gagaaaccgt
    10561 gtcaaagggg caaaaaatag ctgaaatggg caacaccgat agcgaaacgg ttaagcttca
    10621 tttcgagatt agagaacgag gtaagcccgt tgacccactc aaatttttgt caaattagca
    10681 cttagaatga ttatatgatt gggagtcatc agcccttaca tttggtatga gcctgcctag
    10741 ctagagatta cttctgagag atggaaatag cgcttcttgc cagattttta gtttcttttc
    10801 atagggtatg gaagcattag cggggcttgt tgagggaagt ctgatgaatt gttttggact
    10861 tccgctgatt gttttagcaa cataacgatt aaagcaattt tctgctttac ttccattaaa
    10921 acataccttt gaaatcaggt ggtgttgctg caagaagtgc tgaaggttat ttacagtttg
    10981 tgaattaggc aaaatatttg aatcaaggct accaactcgc ttacatgatc tcaggacatc
    11041 ccagagtgca atatgagctg atttaagttt ttggaccctc gtttcatagg atgaatttaa
    11101 ttcgaactga agcaactcag ccattatttt ccagaataca ttttgtttat gtgcataata
    11161 ttggcctgcc atcagtgatg catttcctgg catacttcct aaaattaaaa cgctagcatg
    11221 gtggtctgcg atgggttcaa aactgaaaat ataagtcatt taagtattag cgtactaatg
    11281 aatatttcct ggtagtttca gcgctatcac tggatctgct agttaatgtt cattattttt
    11341 atctgagtat tcaggaaccc attttgctaa agccatccga acctcggtgt ctgataacgc
    11401 gggctgttcg atgagccatg taatgaatga agttagccat cgttcgtcta ctggatggga
    11461 cagcgtaatt cgaagttttt cgtgctgagt tggtagggtg ctctcattct cagttattaa
    11521 ttcttcatga agcttttcac ctgatcgtaa acccgtataa gtaattttaa tttcttcttc
    11581 actgaatcca gatagtcgaa tcatatcttt agcaagatca acgattctaa taggattgcc
    11641 catatccata acaaaaattt ctcccccccc ttttattccc cccatagaac cggcttgaag
    11701 aacgagttgt gctgcttctt gaattgacat gaaatagcga gaaatatcag gatgagtgac
    11761 tgtaataggt ccccctctcg caatttgctc acgaaactta ggtattacgc tacccgtgct
    11821 acctagaacg ttaccaaaac gtacaatgac aaagcgggta ctaggtgaat aaatggtatt
    11881 ggcttgcata ctctcttgag agagaattga ttgttgcaat gcttggcaaa ccatctcagc
    11941 taatcgcttg ctcgcgccca ttacattggt agggttaact gctttatcag tcgatatgag
    12001 tacaaattta gtgatgtgat gtttgatcgc taatttagct aaaacatagg tacccagaac
    12061 attattttgt aatgcctgcc aagtattttc tttttccatt aatggaacat gcttgtaggc
    12121 agcggcatgg aaaataacag tgggttgata ctgtgcaaaa agctgcgcta atctcgattc
    12181 atctttgaca tcgccaatga cgaaagccat cgcgatatca ggaaatttgc tacaaaattc
    12241 ttcttgaatc gagtagagcg caaattcatt tagctcaaat aaaactagtt gtttcggttt
    12301 gaaggtgatg atttgtctgc aaagttctga tccaatcgag ccgcctgcac ctgttatgag
    12361 tatggttttg tttgataaca gggtatgtaa accttcagta tcgagaataa caggctcacg
    12421 accgagtaaa tcatcaagtt caatatctcg aatttgcgaa acagtcattt ttccactgac
    12481 taaatcagtg taagatggaa tcgtcattgc ttttacgcct acttccgagc atatttcaag
    12541 ggcctgacgg cgttcattag tctgagtggg tggcatagca atgatgatat gcctaacact
    12601 taatttttct acccagtaat cgagttcatt gatttttcct agaacgcgca caccatgcaa
    12661 tcttgatcct tgctttttag gactgtcccc cattaatcct acaacctgcc aatctgggct
    12721 atgagcaagt tccttgacga ggttcactgc catgctccca gtcccaagga taatgactgt
    12781 gttacgttcg gaatttttaa agctatataa gcgttgctct ttccaggatc gatacagtat
    12841 tcgactgcct cccataatga gcattaatag caaaggtgca agaacgatca cagcacgagg
    12901 aacaacaccg ggtagcttca gcatgaataa aacaaatgga actaccattg cgcttaacgt
    12961 aacagcccaa aagatacgtt ttaaatcagg tatgctagcg taacgccata tacctcggta
    13021 gaggcggaat aaataaaaga acgaagcttg tagtggaacg atccacaata aattttctac
    13081 gagtgatgct tggaaatggg gggggatttc gaagttaaaa cgaaataagt aagctaacca
    13141 ccaagcgatt atgattgcca aaaaatcatg aatgaacgca atcagggggc gtaattgaaa
    13201 tttacttgcg accattctag ttaacgatta tggtgagatt gatgccgatc gaaagcatgc
    13261 attataacta aataagctat tccccacatg ctacttatcc agctttgctg agcaaactca
    13321 aattttgcag agagtatggc agtacctcca acgactagca tcaatatata agcgagtaaa
    13381 gctgtattac ggtgtccaag accacctttg attaatcgct gataataatg ttcattatgg
    13441 gcttgccaaa ttttcttgcc acgaagtaaa cgcttgatga acgtcactgt ggcatctgct
    13501 ataaagggtg agaagatcaa caatggcaac cataatgacc ataggttatt tttccaaccc
    13561 agcaggccca atgcgccagc caaaaatccc agagagattg caccgacatc acccataaat
    13621 atctttgcgg ggtaaaaatt gaatcgtaaa aacgccaatg ctgaagctgc aattgaaaaa
    13681 ttgatggtgg cgaaatcaat atctccagct agtgtcgctg cgattccata gtaaccaaaa
    13741 ccaatagccg tcattcctcc tgcaagccca tcagatccat ccatgaagtt atataaattt
    13801 gttatccaaa tgataccgag accaaccgta atcacgaata accagctata tgtatccaag
    13861 aataagttaa caaccagcag cattgctgca atcgtatgga tcgataatct taaccagaca
    13921 ggtaaaggct taatatcatc agcaaaagat attgctgaca caaaagctaa tccaataaat
    13981 acgggcactg aaaaaatatc gacaaacaat aacccagtga tgaagatggc tccaattaaa
    14041 cccaacccac ctgtgcgagg gatcggttta gcatgcaagg aacggtcgtt gggtatatcg
    14101 attatattga gtgagttccc aagtctaatg agccagaaga tcgtcagata agcgatgaga
    14161 aaagtaagaa tgggtgcgac aaataattga aacggaagtc ccaagatcat gtgaacggga
    14221 ttatcctaaa aagtcaaaag aaatagttca atcgagaaaa atagaatggt ttaaacctaa
    14281 attggaatca gaatttgtta actaacatta acgaat
//
LOCUS       DAOQKQ010000054        14217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_1994_length_14217_cov_5.698489, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000054 DAOQKQ010000000
VERSION     DAOQKQ010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 14217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14217
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1994_length_14217_cov_5.698489"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PKZ49_05065"
     CDS             complement(<1..302)
                     /locus_tag="PKZ49_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HND35968.1"
                     /translation="MLGQSLKYLSTQLNDKRISSVELTTEYLKQIRSLNTEFNAFITI
                     DEAQSLEQARQADQLIATGNASPLTGIPIAQKDIFCTKGWLTTCGSKMLANFTAPY"
     gene            complement(352..639)
                     /gene="gatC"
                     /locus_tag="PKZ49_05070"
     CDS             complement(352..639)
                     /gene="gatC"
                     /locus_tag="PKZ49_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HND35969.1"
                     /translation="MEILLTDVYRIADLARVEIQTDEAQAALEQLSNIFNLIEQMQAV
                     DTAEIEPMSHAQDMKLRLRQDIVSEADRHELFQSIAPQVEAGLYLVPKVIE"
     gene            786..1844
                     /locus_tag="PKZ49_05075"
     CDS             786..1844
                     /locus_tag="PKZ49_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HND35970.1"
                     /translation="MFNLLNNNFLGNYFSTDMAVDLGTANTLIHVRGRGIVLNEPSVV
                     AIRQEGGSSGRKVIQEVGLAAKQMLGRTPGNITAIRPMKDGVIADYTVTEQMLKQFIR
                     KVNPPRLFSANPRIVISVPCGSTQVERRAIREAAIGAGARKVELIEEPMAAAIGAELP
                     VETPTGSMVVDIGGGTTEVAVISLGGVVYSNSVRVGGDKFDEAIINYIRRNYGMMVGE
                     ATAELIKTQIGSAYPGSEVRDIEVKGRNLAEGIPRSFTISSNEIMEALTEPLNNIVSA
                     VKTALEHTPPELGADIAEMGMVMTGGGALLRDIDRLLMEETGLSVVVADDPLTCVVRG
                     AGIALENIDRSIGIFARD"
     gene            1954..2835
                     /gene="mreC"
                     /locus_tag="PKZ49_05080"
     CDS             1954..2835
                     /gene="mreC"
                     /locus_tag="PKZ49_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HND35971.1"
                     /translation="MESVPQFFRTGPSPQARLFFFSLLSILLMIADLRFESLHKVRQA
                     IGVILYPLQKIALAPASFYDQVEALIEDLRLTKEINYLRQQYFLYQQQFSRLQILEEE
                     NTHLRRLLNAAKQTEVKSIMAEILYIPRDPFKRKIILNRGASDGIDAGQVVVDDLGVI
                     GQVTQIFPWSAEVTLITDKDHAVPIQVARNGLRSVISGVGKNNELELRYLSINADIQV
                     NDLLVTSGIGGIYPPGLPVAKVEKIEHDRSYNFSRITCAPIAGVDRNRHVLILSIPPS
                     EPESTTNNNTVESKSSN"
     gene            2847..3365
                     /gene="mreD"
                     /locus_tag="PKZ49_05085"
     CDS             2847..3365
                     /gene="mreD"
                     /locus_tag="PKZ49_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HND35972.1"
                     /translation="MSKDYLEKELPLEAKSRFIFISLVIAFIINLFPLQEIIFLLRPD
                     FVALTLLFWNINQPHHVGMSLAFTLGIIMDVCNSSILGQHALAYCFMSFLAIILHRRL
                     VLFSVFQQAPQIFWILLLTQVIIYISGTLTGSYHSDWYFFLSSVTGALIWPVIALILG
                     ISQKPETDPNAL"
     gene            3362..5260
                     /gene="mrdA"
                     /locus_tag="PKZ49_05090"
     CDS             3362..5260
                     /gene="mrdA"
                     /locus_tag="PKZ49_05090"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HND35973.1"
                     /translation="MNQVIELRNHLLELRHFHLRLVVAAIFVVLLFLILFSRFFYLQV
                     LRSDHFTTLAEANRITILPLVPNRGLIYDRHGEILAQNLTTYVLEIVPDKIRNLDSAI
                     DELSKLIEITPVDRRRFEKLRKENTRFKSLPIRTRLSDAEVARFAANRYRFPGMEINA
                     RLLRHYPHAEMVSHVIGYIGRISDKDLEILESNNALENYRGSSHIGRIGIEQSYEKEL
                     HGITGFEQVETDVAGRSVRTLSRTLPIAGSDLILSLDLKLQQAAERAFGEYRGSLIAM
                     DPNTGEILAFVSKPGFDPNLFVDGIDHLNWKLLNESIDKPLNNRALRGVYPPGSTYKP
                     FMALAALELGKRSPDYAINDPGFFSLPGSSHRFRDWRVGGHGRVNLHKSLVVSCDTYY
                     YMLANDLGINAIYNFMGQFGFGKKTEIDIPGEIVGLSPSPEWKMKRFKQNWFPGDTIS
                     IGIGQGYNLATPLQLTFATMILANNGHAFRPHFVKQIHDSKTGRIYENMIKQLYPLNL
                     KNENLMRVRNALVDVTRPGGTAARAGANASYTFAGKTGTSQVINIKQGERYVASKINE
                     RHRDHAMFIAYAPAEDPKIVVTVLVENGGSGGSTAAPIARKVFDYYLLGQMPEPLQSD
                     SVNNSRRD"
     gene            5329..6432
                     /gene="rodA"
                     /locus_tag="PKZ49_05095"
     CDS             5329..6432
                     /gene="rodA"
                     /locus_tag="PKZ49_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HND35974.1"
                     /translation="MDSIDRIWQYCTRYIDGFLLGAIFLLMITGLIVLYSASGGNFGR
                     VNSQFVNMLIAVFIMWVIANIPLQQLMRLALPLYFIGIILLIAVALFGEVQNGARRWL
                     NIGVTRIQPSELLKIAVPLMMAWYFEKTEIKLRLRDYAGGALILILPVLLIARQPDLG
                     TAILISASGFYVLYFAGLSWRVMGSLAIIAAAAIPLFWSFGMHDYQRKRVLTMLDPSQ
                     DALGAGYHIIQSNIAIGSGGITGKGWQNGTQSQLDFLPEQSTDFIFAVFSEEFGLMGN
                     SLLLLLYLIIIGRCIYITANAPTQFTRLVAGSITLTFFTYVFVNMGMVSGILPVVGVP
                     LPLISYGGTSLVTMLLGFGILMSIQTHPKLVKT"
     gene            6444..7349
                     /locus_tag="PKZ49_05100"
     CDS             6444..7349
                     /locus_tag="PKZ49_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HND35975.1"
                     /translation="MHFKKDFFNSSLNWLSFFLVITLLYGCSSPAPFVRSGEKISMAP
                     VTSPTESQNKNYYLDDGPEDDPPTNLDTIPSANPQPEAIRPANMRPYMVFGQVYTPMT
                     SIEPYKQKGIASWYGRRFHGKPTASGEVYNMYGMTAAHPTLPIPSYARVTNLNNGKSI
                     VVRINDRGPFIANRLIDLSYTAAYQLNILTNGSGPVEVESIIPQSDQFASNRANQAPK
                     ISPINPTNQIFLQLAAFGSKHNADKYLLQIRQELATIGENINITQEGNLYKVHIGPYA
                     NHASAKEIAHEILQRTTIKPLLTMR"
     gene            complement(7372..9717)
                     /locus_tag="PKZ49_05105"
     CDS             complement(7372..9717)
                     /locus_tag="PKZ49_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HND35976.1"
                     /translation="MAGVCWLQQQATLPESSAIWILPLLVVLIAGFANYSTRLARFIN
                     AFLIFCFFLTLGFLWAGVNAHWRLADELPEEWESRDIAVTGVIAKLPEITRQGIRFQF
                     DIEKIQTEGAYVPKHILITWYQNQHRAIKIQPGERWQLTVRLKRPHGNANPYVLDTEI
                     KYLERNIRAIGYVRYSDQNIRLDEEVRTPFYFIERLRDQIRKRFNEVLSDHPYSGVLI
                     ALAIGDQRAIPKDQWTLFIQTGTSHLMAISGLHVTMISGIAFTFFYWLWCRFPQLLVR
                     LPARKFALSAGLVIALGYVLLSGFAIPARRAFLMLCVIVIGLWQDRRIAVSTLLKWAL
                     IIVVMFDPWAVTSPGFWLSFGAMAWIAYIAIDRIGSVQTNKISNWITIQWVITIGLVP
                     LLLILFQQFSLISPVANALMIPLVSLAVVPLTVVSVMPAFDFLLIPAHLALTGGMTLL
                     QGLSELTDTTWQQHQPPLWAIIAGMLGIFWFFLPGGRGLFLTSGFPSRWLGIMGLLPL
                     FLISPPKPSQGELWVTVLDVGQGLSIVVQTENHVLLFDTGPRYGDSNSGERIIIPFLR
                     GAGIQHIDQLVISHADSDHSGGLSSILGEVPVKTIVSSVELDHPVLSAVPQSQPCRAG
                     DAWIWDGVLFEFLYPLSPERKQRVQRSRQNNSSCVLKITTAGDSILIPADIEKEAELD
                     LLKYFSHLLPSTVLIAPHHGSKTSSQAAFIQSVNPQLTIFTVGYRNHLKHPNQAVVER
                     YQAIGSQMMRSDYQGAITLQFSQHGFTAKSWRETKPRYWYQ"
     gene            9914..10600
                     /gene="rpe"
                     /locus_tag="PKZ49_05110"
     CDS             9914..10600
                     /gene="rpe"
                     /locus_tag="PKZ49_05110"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HND35977.1"
                     /translation="MFRIAPSILSANFAKLGEEITNVLDSGADIIHFDVMDNHYVPNL
                     TIGPLVCEAIRPLTKAMIDVHLMVEPVDRIVPDFAKAGADIISFHPEASNHIDRTIGL
                     IKEQGCKAGLVFNPATPLSYLDHILDKLDLILIMSVNPGFGGQKFIPEALNKLRAVRQ
                     RIEASGKKDILLEIDGGVKVDNIAEIARAGADTFVAGSAIYGAGKDSDPHRYDTVLAA
                     MRSELAKVKA"
     gene            10661..11386
                     /locus_tag="PKZ49_05115"
     CDS             10661..11386
                     /locus_tag="PKZ49_05115"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HND35978.1"
                     /translation="MTHSASSQQIYLKKEIANPLAIKAVVIDLDGTLLDTAMDLATAA
                     NLMLVELGKPELSFATIQTFIGKGIQKLVKRALTNHLDVEPDPALFEKALPIYEHHYR
                     ENLSKQTRPYPGVVEGLKILQQKGFRLACITNKAEAFTVPLLKATHLFDYFELVLSGD
                     SLPRKKPDPLPLLHACQFFQIEPHQLLLIGDSLNDAIAARAAGCAIFCVPYGYNEGQD
                     VKALDCDAIVPSIAAATEIIENA"
     gene            11443..12921
                     /gene="trpE"
                     /locus_tag="PKZ49_05120"
     CDS             11443..12921
                     /gene="trpE"
                     /locus_tag="PKZ49_05120"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HND35979.1"
                     /translation="MNEDKFNELAAQGYNYIPFVLETFADLDTPLSIYLKLANQPFSY
                     LLESVLGGERFGRYSVVGLPARTRLEVFKQQINIIKGTATETHHVTDVLGFIESYLEN
                     YKIAPNDDFDLPRFYGGLVGYFGYDTIRYIEPKLSNCSKPDILNTPDILLLLSEELVV
                     IDNLTGKIYLIVFINPNTDHAYQTGKDRVKALLQQLRQPVTIPSESPHSPSTATSEFS
                     ESAFIAAVERAKQYIIDGDIMQVVLSQRTSLPYTASPLALYRALRSLNPSPYMFYYHL
                     GDFHIVGASPEILVRLEKNVVTVRPIAGTRPRGKHFQEDQALATELLADPKERAEHVM
                     LMDLGRNDISRVAQTGTVKVTENMTIERYSHVMHIVSNVEGQLEPNLTAIDVLRATFP
                     AGTVSGAPKIRAMEIIDELEISKRGIYAGAVGYLGFNGDMDLAIAIRTGVIKDGKLYV
                     QAGAGIVADSIPYNEWVETQNKAKAVLRAAEIAEKGLDSKIN"
     gene            12951..13042
                     /locus_tag="PKZ49_05125"
     tRNA            12951..13042
                     /locus_tag="PKZ49_05125"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12985..12987,aa:Ser,seq:gga)
     gene            complement(13712..13867)
                     /locus_tag="PKZ49_05130"
     CDS             complement(13712..13867)
                     /locus_tag="PKZ49_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND35980.1"
                     /translation="MSSLYRFLHHSFGIAIISNSFQLMVTIFCLLGGYITQETLNDVW
                     DITGEAI"
BASE COUNT         4149 a         3132 c         2879 g         4057 t
ORIGIN      
        1 tcatagggcg ctgtaaaatt agctaacatc tttgacccac acgtagttaa ccaaccttta
       61 gtacaaaaaa tatctttctg agcaattgga atccccgtca gcggtgaagc atttcctgtc
      121 gcaattaatt gatccgcctg tcgcgcctgc tcaagactct gcgcttcatc gatcgtaata
      181 aacgcattga attcagtatt cagtgaccgg atttgcttaa ggtattctgt tgttaattca
      241 acactcgaaa ttcttttatc gtttaactga gtagataaat acttaagact ttgacctaac
      301 ataacacttc taaaataagg aaaaaaatag cgaggaagta ttccgcacga attattcgat
      361 gacctttgga actaaataaa gtcccgcttc tacttgcggt gcaatcgatt ggaaaagttc
      421 gtgccgatcg gcttcagaaa caatatcttg acgtaaacgc aatttcatat cttgggcatg
      481 actcatcggt tcaatctctg cagtatcaac cgcttgcatc tgttcaatta aattgaaaat
      541 attggataac tgctctaacg ctgcttgagc ttcatcagtt tgaatttcaa ctcgagctag
      601 atccgcaatc ctatagacat cagttagtaa aatttccatc tgtaattcgg cgctttatct
      661 taaaatgggt aatagagtat catgacatat ctgatgaacg catatatttt acacgtcaca
      721 ccttaacatc gctgcaaaag catttattta tcaaaatctg tcatctttaa tggaaattcg
      781 ccaatatgtt taacctcctg aacaataact ttttagggaa ctacttttca actgatatgg
      841 cagtcgattt gggtacagcc aataccttaa ttcatgtccg ggggcgcggt attgttttga
      901 acgagccctc tgtcgttgca attcggcaag aaggtggctc aagtggtaga aaagtcatcc
      961 aagaagttgg tttggctgca aaacagatgt taggaagaac acctggaaat attactgcca
     1021 ttcgacccat gaaagatggt gtaattgctg attacacagt aactgaacaa atgctcaagc
     1081 aatttattcg taaagtaaat ccacccagac ttttttctgc aaatccccgt atcgttattt
     1141 cagttccttg tggttcgact caagttgaaa gaagagcaat tcgagaagct gccattggtg
     1201 ctggagccag aaaggtggaa ctcatagagg aaccaatggc agcagctatt ggagcagaat
     1261 tacctgtaga gacccctact ggatcgatgg tcgtcgacat cggtggtgga acgaccgaag
     1321 tcgctgttat ttccttaggg ggtgttgttt actctaattc cgttcgtgta ggtggcgata
     1381 aatttgatga agccattatc aattatattc gccgcaatta tggcatgatg gtcggtgaag
     1441 caacggctga attaattaaa acgcaaattg gttccgctta ccctggttct gaagtgcgcg
     1501 atatcgaagt caagggacga aatttggctg aagggattcc ccgcagcttc acgatttcta
     1561 gtaatgaaat catggaagca ttaactgaac cgctcaacaa catcgtcagc gctgtaaaga
     1621 ctgcgttaga acacacgcca cccgaattag gtgctgacat tgcagaaatg ggtatggtca
     1681 tgacgggagg aggtgcgcta ctccgtgata ttgatcgatt gttaatggaa gaaactggat
     1741 tatcggtcgt ggtagcagac gatcccctaa cctgtgtggt tcggggagct ggtattgcat
     1801 tagaaaatat tgaccgctct attggtattt ttgctcgcga ttagtaaatg catcaccaaa
     1861 catggtaacg cattgtcaca tcaacttaag taaaatcggt gtttacatca aacaaacgca
     1921 gaatatattt tttagtctag aacagggcga ttaatggaat cagttccaca gttttttaga
     1981 acaggcccca gcccacaagc tcgtttgttc tttttttcct tgctatctat tttactcatg
     2041 attgctgatt taagattcga aagcttacat aaagttaggc aagcaattgg tgtcattctt
     2101 tacccgttgc aaaaaattgc attagcgcca gccagtttct atgatcaagt cgaagctctc
     2161 attgaagatt tacgtttaac gaaagaaatt aattatctca gacaacaata ttttctttat
     2221 cagcagcaat tttctcgact acaaatccta gaagaagaaa atacacattt gaggcgatta
     2281 ttaaatgctg caaaacaaac tgaagttaag tcgatcatgg cagaaattct ttatattcca
     2341 cgtgatcctt tcaaaagaaa aatcattttg aacagaggcg cctcagacgg aattgatgca
     2401 ggtcaggttg tcgttgatga tttaggtgtc attggacaag taacccaaat ttttccatgg
     2461 tctgccgaag tcaccttaat cacggataaa gatcatgcgg ttcccatcca agttgcgcga
     2521 aatggattgc gttcggttat ttctggcgtt ggcaaaaaca acgagttaga gttacgttat
     2581 ttatccataa atgcggatat acaagtcaac gacttgttgg tcacgtcagg aattgggggg
     2641 atttatccgc caggattacc tgtcgcaaaa gtagaaaaaa ttgagcacga tcgatcttat
     2701 aatttttctc gaattacttg tgcacccatt gcaggcgtcg ataggaatcg acatgtatta
     2761 atcctttcca taccgccatc cgaaccagaa tcaacgacta ataacaatac tgttgaatca
     2821 aaatcatcaa actaaaggca aatagcatgt ccaaagatta tctcgagaag gaattaccgc
     2881 tagaagctaa gagccgcttc atattcatta gtttagtgat tgcattcatc atcaatctat
     2941 ttccgttgca agaaatcatc tttttactac gacctgattt tgttgcactg acattacttt
     3001 tctggaatat taaccagccc caccacgtag gaatgagtct tgcttttact ttaggaatta
     3061 tcatggatgt atgcaatagc agcatcttag gtcaacatgc gctcgcttat tgctttatgt
     3121 catttcttgc aattatccta catcggcgct tagtattatt cagtgtcttt caacaagcac
     3181 cccaaatctt ttggatattg ctacttactc aagtgattat ctacatttca ggcacattaa
     3241 ccggttcata ccactccgat tggtatttct ttctttccag cgtaacaggc gccttaattt
     3301 ggccagtcat cgctttgata ttaggtattt cacagaagcc tgaaacagat ccgaatgcgt
     3361 tatgaatcaa gtcatcgaat tacgtaacca tttacttgag ttacgtcatt ttcatctaag
     3421 gcttgtcgtc gctgctatct ttgtggtgtt gttattttta attttatttt cgcgtttctt
     3481 ttacctacaa gtccttcgaa gcgatcattt caccacccta gctgaagcaa accgtatcac
     3541 tatcttaccg ctggttccca atagagggtt aatttatgat cggcatggtg agattcttgc
     3601 gcaaaattta accacctatg ttttggaaat tgttcctgac aaaatacgca atttagattc
     3661 ggcaatcgat gaactgtcca agctcattga aataacaccc gtcgatcggc gacgtttcga
     3721 aaaattacgc aaagaaaata ctcgattcaa aagtctaccg attcgaactc gcttatctga
     3781 tgcggaggtt gcacgttttg ccgccaaccg ctaccggttt cccggtatgg aaatcaatgc
     3841 acgcttacta cgccactacc cgcatgcaga aatggtttcg catgttatcg gctatatcgg
     3901 gcgtattagc gataaagatc ttgagatact tgaaagcaat aatgcattag aaaattatcg
     3961 tggatcttct catattggaa gaattggaat tgagcagagc tatgaaaaag aattgcatgg
     4021 cattaccgga tttgaacagg tagaaaccga tgtagcaggt aggtcggtca gaacgttatc
     4081 gcgaacactt cccattgctg gaagcgattt aatcctttcc ctggacttaa aactgcaaca
     4141 agcagcagaa cgtgcctttg gtgaatatcg tggctccctc attgcaatgg atcccaatac
     4201 cggtgaaatc ttagcatttg tcagcaaacc cggatttgat cccaatttat ttgttgatgg
     4261 gattgatcat ctcaattgga aactactgaa tgaatcaatc gataaacccc tgaataatcg
     4321 tgcattacgc ggcgtttatc cgcccggctc tacctataaa ccctttatgg cgctggctgc
     4381 attagaatta ggtaaacgat cgccagatta cgccatcaat gatcctggtt tttttagttt
     4441 accgggtagc tcacatcgct tccgcgattg gagggttggt ggtcatggta gagtcaatct
     4501 gcacaaatcg ctcgttgtct cctgcgacac ttattactat atgctagcaa atgatttggg
     4561 tattaacgct atttataatt tcatgggtca atttggattt ggtaaaaaaa ctgaaattga
     4621 tattccaggt gaaattgttg gattatcacc ctcccctgag tggaaaatga aacgcttcaa
     4681 acaaaattgg tttccaggtg ataccatatc gattgggatt gggcaagggt acaatttagc
     4741 aaccccgctg caattaacgt ttgccactat gatccttgca aataatggcc atgctttccg
     4801 tccccatttt gtaaagcaaa tacacgacag caaaacgggg cgaatctatg agaatatgat
     4861 taaacaactt tatcccctca atctcaaaaa tgaaaatttg atgcgagtgc ggaatgcttt
     4921 ggttgacgta acacgccccg gaggtactgc ggcaagagcg ggcgccaatg ccagttatac
     4981 ctttgctgga aaaacaggca cttctcaagt catcaatatc aaacaaggtg agcgttatgt
     5041 agcaagcaaa ataaacgaac gccatcgcga tcatgcgatg tttattgctt atgcgccggc
     5101 tgaggaccct aaaattgtag tcacagttct cgttgaaaat ggtggttctg ggggatcgac
     5161 tgccgcacct attgcgcgga aggtttttga ttattatcta ttaggacaaa tgccagagcc
     5221 attacaatcg gatagcgtca ataatagccg ccgtgactaa tttctggtta atcgtattca
     5281 cgatttacga tcgttccaat aacccattaa cctattgact tctctcgaat ggatagcatt
     5341 gatcgaatct ggcaatattg cacacgctat atagatggct tcttactcgg tgccattttt
     5401 ctgctgatga taaccgggct gatcgtactc tacagcgcaa gtggcggtaa tttcggtcga
     5461 gtaaatagtc agttcgtgaa tatgctgatc gcagttttta ttatgtgggt tatcgcaaat
     5521 atcccgctcc aacagctcat gcgattagcg ctaccgttgt attttatcgg tatcatctta
     5581 ttgattgctg tggcactttt tggtgaggtt caaaatggtg cgcgacgttg gttaaatatt
     5641 ggcgttaccc gtatccaacc ttctgaactt ctaaaaattg cggtgccact gatgatggct
     5701 tggtattttg aaaaaacaga aataaagtta cgtcttcggg attatgcagg cggtgcgctt
     5761 attttaatat taccagtact actcattgcg cgccaacctg atcttggtac ggcaatatta
     5821 attagtgcga gtgggtttta tgtactctat tttgcgggat tatcttggcg agtaatgggc
     5881 agtcttgcga tcatagctgc tgcagcaatt ccattatttt ggtcattcgg gatgcacgat
     5941 tatcagcgta aacgggttct aacgatgcta gacccatccc aagacgcact tggcgcgggt
     6001 tatcatatta ttcaatcgaa tatcgccatt gggtcgggtg gtattaccgg gaaaggatgg
     6061 cagaatggta cacaatctca actcgatttt ttaccggagc aaagtacaga tttcattttt
     6121 gctgtattct cagaagaatt tggattgatg ggcaatagct tattactgtt actttatctc
     6181 atcattatcg gacgttgcat ctatataact gccaatgcgc ccacgcaatt tacgcgcctt
     6241 gttgccggtt cgattacgct gacttttttt acatacgtat ttgttaatat gggcatggtc
     6301 agtggtattt taccggttgt gggtgttcca cttccattaa ttagctatgg tggtacatca
     6361 ttggtcacga tgctcctggg ttttggtatc ctcatgagca tccaaactca ccctaagcta
     6421 gtcaagacat gaggtacctt caaatgcatt ttaagaaaga tttttttaat agcagcctaa
     6481 actggcttag tttcttctta gttatcaccc tactttatgg gtgtagcagc cctgctccct
     6541 ttgttcgttc tggcgaaaaa atttcgatgg cccccgttac atcaccaaca gaatctcaaa
     6601 acaaaaatta ttatctcgat gatggccctg aagatgatcc ccccacgaat ctagacacca
     6661 tccctagcgc aaatccccaa ccagaagcaa ttagacctgc taacatgcga ccttatatgg
     6721 tttttggaca agtctataca cccatgacga gtatcgagcc ttacaaacaa aaaggcattg
     6781 catcttggta tggccgtcgt tttcacggta aaccgactgc ttcgggtgaa gtttataata
     6841 tgtatggtat gacagctgct cacccgactt tgccaatccc aagctacgct cgcgtgacaa
     6901 acctaaataa tggaaagtcc atcgttgttc gaatcaatga ccgggggcct ttcattgcaa
     6961 accgactcat agacctatct tatacagcag cctaccaact taatatcctg acgaatggca
     7021 gtggaccagt cgaggtagaa agcatcattc cgcagagcga tcaattcgct tcaaatcgtg
     7081 caaatcaagc tcctaaaatc agtcctatca atcctaccaa tcaaattttt ctacaattgg
     7141 cagcgtttgg ttcaaaacat aatgcagata aatatctgct acaaattcgc caggaactcg
     7201 caaccattgg tgaaaatatt aacatcacgc aagaaggcaa tctatataag gtccatattg
     7261 gcccctatgc taatcatgca tcagccaaag aaatcgctca tgagattcta caacgcacga
     7321 caattaaacc tttgcttacc atgcgttaat gattcgatga cccgtaccac gtcactgata
     7381 ccagtaacgt ggtttcgttt cccgccaact cttggcagta aaaccgtgtt gtgagaattg
     7441 cagtgtaatc gccccttgat aatcactgcg catcatctga cttccaattg cctgataacg
     7501 ttcaaccact gcttgattag ggtgctttag atgattgcga taacccaccg taaagatagt
     7561 taattgcgga ttgacgcttt gaatgaaagc agcttgagaa gaagttttac ttccatgatg
     7621 cggtgcaatc aaaacggttg atggcaataa atgagaaaaa tattttaata aatctaactc
     7681 agcttctttt tcaatatctg ctggaatgag tatactgtcc ccggcagtcg ttattttcaa
     7741 tacgcagctc gaattattct gcctgcttcg ctgtaccctt tgtttgcgtt caggcgataa
     7801 gggatacaaa aattcaaata aaacaccatc ccatatccaa gcatcacccg ctcgacaagg
     7861 ttgactttgt ggaaccgcag ataaaactgg atggtccaac tctactgatg acacaattgt
     7921 tttgacaggc acctctccta atatcgatga taatcctcca ctatgatctg aatctgcatg
     7981 agatatcact aactgatcta tgtgttgaat tcctgcgccc cgtaaaaagg gaataatgat
     8041 ccgttcgccg ctattgctat ccccataacg cggcccagta tcaaacagta gcacatgatt
     8101 ttctgtttga accacaattg ataacccctg cccgacatct aataccgtta cccataattc
     8161 gccttggctt ggtttcggtg gtgaaatgag aaacaaaggt agtaaaccca ttatgcctaa
     8221 ccatcgcgaa ggaaagcccg aagttaagaa cagtcctctt ccacccggta gaaaaaacca
     8281 gaaaattcct agcattccag ctatgattgc ccataaaggg ggctgatgct gttgccaagt
     8341 ggtatctgtt aactcactga gtccttgcag taatgtcatc ccccctgtta atgcgaggtg
     8401 agcaggtatc agtaaaaaat cgaatgctgg cataactgaa actaccgtga gtggcacaac
     8461 cgcgagactg acgagaggaa tcattagcgc attggcaact ggagatatca gcgaaaattg
     8521 ctggaacaaa atcagcaaca atggaacaag cccaatggtg ataacccact gaattgtaat
     8581 ccagttactg atcttattgg tttgtacact accaattcga tcgatcgcaa tataggcaat
     8641 ccaagccatt gcaccaaacg ataaccaaaa ccccggtgat gtaacagccc aaggatcgaa
     8701 catcactacg atgatcaacg cccatttaag tagcgtagaa actgcaatgc ggcgatcctg
     8761 ccataatcca attacaatca cgcataacat taagaatgca cggcgcgctg gtatagcgaa
     8821 ccctgagagc aatacatacc ccaatgcaat gaccaatcct gcgcttaatg caaatttacg
     8881 agcaggtaat cgtactaaga gctgcgggaa gcgacaccaa agccaataga aaaaagtaaa
     8941 ggcaatccct gaaatcatcg tcacatgaag tcctgaaatt gccatcaagt gactcgtgcc
     9001 cgtttggata aataatgtcc attgatcttt aggaatagcg cgttgatcac cgatagcaag
     9061 cgcaatcaaa accccactat aaggatgatc cgatagtact tcattaaacc gcttgcgaat
     9121 ctgatcccgt aagcgctcga taaaataaaa tggtgtacgt acttcttcat ccagacgaat
     9181 attctggtct gaataccgta cataaccaat cgctctaata ttacgttcta ggtattttat
     9241 ttctgtatcg agcacataag gattggcatt tccatgagga cgtttcaacc gaactgtcaa
     9301 ttgccatcgt tctccaggtt gaattttgat cgccctatgt tgattctggt accaagtaat
     9361 taaaatatgc ttcgggacat aagcgccttc cgtttggatc ttttcaatat cgaattgaaa
     9421 gcgtatcccc tgtcgagtga tttctggcaa ttttgcaatc accccggtca cagcaatatc
     9481 acgactttcc cattcttctg gcaattcatc tgcaagacgc caatgcgcat taacccctgc
     9541 ccaaagaaaa cctaaggtaa gaaaaaaaca aaaaataaga aacgcattga taaatcttgc
     9601 gagccgagta ctgtaattag caaacccagc aatcaaaacg actaataacg gcagtatcca
     9661 aattgcactg ctttccggta aagttgcttg ctgctgcagc caacaaacac cagccacgaa
     9721 tcctaaactt ttaaaatgat ttggtaattt cgtaacgatt tcgatcaacc tagcttttgt
     9781 aagcatggaa acgcatgtga aacatcacgt ttgaaaataa aaataaagga agttttaaaa
     9841 ttcacacgac tgacattaac gcgaaatgac aatttacggt taaaatgcac tatttaataa
     9901 taagaggcta gtcatgttcc gaattgctcc cagtattttg tctgcaaatt ttgcaaaact
     9961 cggtgaagaa atcaccaatg tattagattc cggcgcagat attattcact tcgatgtaat
    10021 ggataatcac tatgtaccca atttaaccat cggcccactc gtctgtgaag cgattcgccc
    10081 actcacaaaa gcaatgatag atgttcattt aatggtggag cctgttgatc gcatcgttcc
    10141 tgatttcgct aaagcgggcg ctgatatcat ttcatttcac ccggaagctt ctaatcatat
    10201 cgatcgtacg attggtttaa tcaaagaaca aggttgtaaa gcagggttag tctttaatcc
    10261 ggctacgcca ttgagttacc tagatcatat tttggataaa ctcgacttga ttttgattat
    10321 gtccgttaat ccaggttttg gtggtcaaaa atttattcct gaggcactca ataagcttcg
    10381 tgcagttcgc caacggattg aagccagcgg caagaaagat attttgcttg aaatcgatgg
    10441 cggcgtgaaa gtcgataaca tcgctgaaat cgcccgtgca ggtgcggata ccttcgttgc
    10501 aggatctgcc atctatggcg ctggaaagga tagtgatcct catcgctatg atacggtact
    10561 cgctgccatg cgcagtgaat tagcaaaagt aaaagcttaa agtaaaccgc gttaagggat
    10621 aactgttgaa cgcattcaat cataacaatc aatattcttt atgacacatt ctgcttcctc
    10681 tcaacagatt tatcttaaaa aggaaattgc taacccatta gcgattaagg ctgtcgtcat
    10741 cgatcttgat ggaactttgc tggataccgc catggatctg gcgacagcag ccaatctcat
    10801 gttagtcgaa ttaggcaaac cagaattatc atttgcgacc atccagacat ttattgggaa
    10861 agggattcaa aagcttgtta agcgtgcttt aaccaatcat ctcgatgttg aacctgatcc
    10921 agccttattt gagaaagcgc ttccaattta tgagcatcac tatcgagaaa atctcagcaa
    10981 acagacccga ccttaccctg gtgtggtaga agggttaaaa atcctacaac aaaagggttt
    11041 ccgtttagct tgtatcacga ataaagctga agcatttacc gtgccgctat taaaagcaac
    11101 ccatttattc gattattttg agcttgtctt atcgggcgat agtttgccga ggaaaaaacc
    11161 cgatccactc cctttattac atgcatgcca attttttcag attgaaccgc atcaattatt
    11221 actcattgga gattcattaa atgatgcgat tgcagcgcga gcagcgggtt gtgcgatttt
    11281 ctgcgtacct tatggttata atgaagggca ggatgtaaaa gcactggatt gtgacgcgat
    11341 tgtgccatcc attgctgctg caacagaaat cattgagaac gcatagcaac caactaatcc
    11401 agcacgattt aacctctatc atactgcgaa gcagcgtaaa tcatgaatga agataaattt
    11461 aacgagctag cagcacaagg ttataactac atccctttcg tgctcgaaac cttcgctgat
    11521 ttagacaccc cgctctcgat ctacctcaaa ttagcgaatc aacctttctc ctatttactt
    11581 gaatcggtac taggaggaga aagattcggt cggtactctg tggtaggctt accagccaga
    11641 acccgtctag aagtttttaa gcaacaaatc aacatcatta agggcaccgc aacagaaacc
    11701 catcatgtaa ctgatgtttt gggtttcatt gaatcttatt tagaaaatta taaaattgca
    11761 cccaatgatg attttgattt gcctcgattc tatgggggat tagttggtta ttttggttac
    11821 gacacgatac gctatatcga acccaagctt agcaactgct ccaagccgga tatcctcaat
    11881 acaccggata tcctgttact cttatccgaa gaactcgtcg ttatcgataa tctcactgga
    11941 aaaatttatc tcatcgtttt tatcaatcct aataccgatc acgcatacca aaccggtaaa
    12001 gatcgagtta aagcgctgct acagcaatta cgacaacccg ttacgatccc ttctgaatcg
    12061 cctcattcac caagtactgc aaccagtgaa ttttcagaat cagcgtttat cgcagccgtt
    12121 gaacgtgcca aacaatacat catcgatggt gatatcatgc aagtcgtgct atcgcagcgc
    12181 accagcctac cctacacggc ttctccgctt gctctgtatc gcgcattacg cagcttgaac
    12241 ccatctcctt acatgtttta ctaccattta ggagatttcc atattgtggg cgcttctcct
    12301 gaaatcctgg ttcgactcga gaaaaatgtg gttacagtac gtcccatcgc tggcacccgt
    12361 ccgcgcggca aacatttcca ggaagatcaa gcgctcgcaa ccgagctatt agctgatcct
    12421 aaagaacgcg ccgaacacgt catgttgatg gatttggggc gcaatgacat tagccgcgtc
    12481 gcccaaaccg gcaccgtaaa agtaaccgaa aacatgacca tcgaacgata ctcccatgta
    12541 atgcatatcg tttccaacgt cgaagggcag ctcgaaccta atttaactgc aatcgatgtg
    12601 ctacgggcca cttttccagc cggtacggtt agcggtgcgc ctaaaatccg ggcgatggaa
    12661 atcatcgatg aactcgaaat atctaagcgc ggtatctacg caggcgctgt aggctacctc
    12721 ggattcaatg gcgatatgga tctagccatt gccattcgca ctggggttat taaggatggt
    12781 aagctatatg ttcaggccgg cgctggtatt gttgctgatt ccattcctta taatgaatgg
    12841 gtagaaacgc agaataaagc aaaagccgtg ttacgggccg cagaaattgc tgaaaaagga
    12901 ctcgatagca aaataaacta acgacacgcc tttcaattta aaatgaattt ggagaagtga
    12961 ccgagaggct taaggtgcac gcctggaaag cgtgtgtacg ctttaaaacg taccgcgggt
    13021 tcgaatcccg ccttctccgc caaacaccca ttatccatgt ccgggtattc gtcaagcact
    13081 tgattcaacc aatatttccc atccgactca ctacagcatt tggcgtggta acgttgatgc
    13141 tgcagaaccg gtatttacga gctgtgttgg cttgcatgaa agcgtgatag tgatcatcga
    13201 ttttaccgat cttggggttc tactgtcacg actttctttt cttcaggttg tgtactcggt
    13261 aaggacaact ctagaacatg ccgacgaccc tgctaccccg gaagtcgatg ggtgacactt
    13321 ccgttatttc atcacccatc caacggcctt ccccttctga ccacagggtc ggctcctcca
    13381 gctcgtttac gaggctacgt gtaggttcac ttgcgttacg gcctgctctc ttgctgtgtg
    13441 gaaacttacg accccgcgtt accacgacgc cgcttcctca tgctaccgac gcgtacggac
    13501 aactcctcgg acgggacttt aacctgctag atttactgct gttactgcga acggtcaagt
    13561 cttgtattaa cacaaatatt tactacctat tcatctactc ttaacagcct aagatttgat
    13621 tagttggaat ttatagctag cggattgggt tagagtaata accacaataa aattgtactc
    13681 gtttcaaaag aactgagtca tcactaaata cttagatagc ttcaccagta atatcccaga
    13741 catcattcaa tgtttcctga gtaatgtagc cacctaataa acaaaaaatt gttaccatta
    13801 attggaaact gttggaaatg attgcgatac caaaagaatg gtggagaaaa cggtagagac
    13861 ttgacatata ctttttctct ctaccatttt taatttggat gctatgaaac ttactggttc
    13921 tgaagcaaaa agaatacttc tcaagaaaat cggtactgat tgtaacaaga aacgaacatg
    13981 atccttattc gtaataattt ctgagaaatt cacttaataa cgagtagtag gttatatatg
    14041 actcgaggag attcaagaaa tttgtggcaa ttcagaataa actgtttatt tgagtactct
    14101 gaattaccta atggataatc tttttaccaa atacaaaata tcagaatggt aaattaattc
    14161 ttacgctttt ttattagatt taaggaagaa gaaggcgata ccggcagcga ttgcaag
//
LOCUS       DAOQKQ010000055        14190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_1999_length_14190_cov_5.913194, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000055 DAOQKQ010000000
VERSION     DAOQKQ010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 14190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14190
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1999_length_14190_cov_5.913194"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..360)
                     /locus_tag="PKZ49_05135"
     CDS             complement(148..360)
                     /locus_tag="PKZ49_05135"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HND35981.1"
                     /translation="MAIRFTVLVLALITLITSCAFGGDASVKVQGTSTITTGQELQDL
                     QRALAENAITQEEYEAAKKALLHRSR"
     gene            complement(381..587)
                     /locus_tag="PKZ49_05140"
     CDS             complement(381..587)
                     /locus_tag="PKZ49_05140"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HND35982.1"
                     /translation="MSKLRITAVNMCFLLVISGCSSTEVVQQPNLNVSIGQQFIDLKK
                     AYDSGALTEKEFQRQKARLIDSIR"
     gene            complement(738..2363)
                     /locus_tag="PKZ49_05145"
     CDS             complement(738..2363)
                     /locus_tag="PKZ49_05145"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HND35983.1"
                     /translation="MTYTHDQTLPKGTHIGVYEIQDTKETTVFDITYRAWNHHLKEQV
                     EILEYFPQKLALRASGSQVVECKNPSNKEDYDLGLRAFLDQGNVLTHVEHPNIAASEN
                     ILEHNGTAYLITHRNEGKPLSRILEPSTIFEEAEIKFILISILNALKELHGYNFIHEG
                     IQPESIFLKKDGEPILTGFSSARWILARRLGEDTSTLITVYSPSEQYESTTEVKPATD
                     FYSLGATLYQCITHKEPVSAQNRLNAIKRGESDPLISLSELSGMTLSPSITKAVDWML
                     KLPCNDRPQSVEELMALIDNVTGKDQAQSNITGAEVVDDFGKHKITKNPLGLAIGVSI
                     AAFIMVGLWFGEKKSDLVDVNKGTDLVEISEPILSSEHHIKDLVEDPFSKTSSENVSA
                     LPDIPLVREDHKVEHSTIESELKGNQQSTMADLSVNTSAQMIVEEPIDKNVIKMHLDA
                     AEKAMRASRFTTPAKDNAFKHYQMVLAIDAHNDIAQAGLRRIVDRYIQFIAKARLESR
                     LEDAQLYLERAGSVLPNDERLEKIRLDLETTAH"
     gene            2763..3755
                     /locus_tag="PKZ49_05150"
     CDS             2763..3755
                     /locus_tag="PKZ49_05150"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HND35984.1"
                     /translation="MKPPVCITVTGAAGQIGYSLIFRIAAGDMLGNDQPIILRLLDVT
                     ESQGFLNGIIMELVDCAFPLLIDVIKTDDPLVAFDKANIAILVGARPRTKGMERKDLL
                     AANSVIFSTQGKALNEVADPNVKVLVVGNPANTNAYIAMKNAPKLPINNFSAMLRLDH
                     NRALSQVAMKINQPVTEIRKMIIWGNHSSTQFPDIDHTEVRGEKIINLISDYKWVENY
                     FLPTVQNRGAAIIEARGGLSSAASAANAIINHMHDWLIGTRENDWVTMGIPSDGSYGI
                     PEEIIYGFPVTCQNGTYRIVQGLEISSRSKAKMLETYQELLEEKDSIKHLLSNR"
     gene            complement(3765..4220)
                     /locus_tag="PKZ49_05155"
     CDS             complement(3765..4220)
                     /locus_tag="PKZ49_05155"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HND35985.1"
                     /translation="MLANPIPDFELESTEGKPFRLTDYRGKKLIIYFYPKDDTPGCTT
                     ESQQFSHLYEQFKALNCEVVGISRDSIKSHENFREKLGLPFDLLSDSNETVCSRFEVM
                     KLKNMYGKQVRGIERSTFVIDEQGKLIREWRGVKVPGHVDEVLSFIKTL"
     gene            complement(4251..5591)
                     /gene="ccmI"
                     /locus_tag="PKZ49_05160"
     CDS             complement(4251..5591)
                     /gene="ccmI"
                     /locus_tag="PKZ49_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HND35986.1"
                     /translation="MTTFWVVAGAFIVTALMFVLPTLIKSRQGKITALERQTANIAIY
                     RDQIAELDADLSNDVLSKEQYEKSKQELQKRMLLDVNASGTESGTDALPSKARGLVTA
                     VVIMLAIPLTAISLYMAIGDTRGLLPQSQLANATQFHSENMAGVPQGHAEIKSVVDNL
                     VARLQDNPEDIEGWVMLGRTYAIMGQYQQASATYAKLVEMIPDNAQLLSDYADVLAMT
                     NNGSLIGRPAELIAHALSIDPEYPKALALAGTVEFEKENYEQAANYWQRLVALIPEDS
                     QLAASVTSSIEQAKTLAVKGKEDHPVQLAQNSKVSSDLQHDATSSDEVKIAANSITST
                     KVSGKVIISPDLASKVSATDVLFVFARAKTGSKMPLAILRLSAKDLPATFSLDDDMAM
                     TPTMKMSSFPEVVIGARVSKSGQAVPTSGDLEGFSQPVKLGDKDVLITIDQIIP"
     gene            complement(5598..6059)
                     /locus_tag="PKZ49_05165"
     CDS             complement(5598..6059)
                     /locus_tag="PKZ49_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HND35987.1"
                     /translation="MSLVFTLVFFVQISFANEAIPVAEDPILEKRLIELSENLRCLVC
                     QNESLAGSRADFANDLRREIREQMKANKSDEEIIDFLVARYGDFVLYNPPFKPTTILL
                     WLGPILLFLGAILALVLYLRRRRSLVFEEPQQLSESQQLEAETLIRKIEKE"
     gene            complement(6104..6628)
                     /locus_tag="PKZ49_05170"
     CDS             complement(6104..6628)
                     /locus_tag="PKZ49_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HND35988.1"
                     /translation="MMRFLLPLAVFIVLVVFLGIGLTLNPRQVPSPLIDKPAPVFELQ
                     QLHDPSKTLASQDNMGKVWMLNVWASWCVACRDEHPLLVQLAKLGIVPIYGLNYKDER
                     STAIQWLKQYGDPYAVSIIDADGKVGIDYGVYGVPETYVIDKKGIIRYKHIGPVTVKS
                     LEDKILPLIKELQG"
     gene            complement(6625..8673)
                     /locus_tag="PKZ49_05175"
     CDS             complement(6625..8673)
                     /locus_tag="PKZ49_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HND35989.1"
                     /translation="MVPELGNFALILAMLLALIQGTLPLIGAARGIPAWIAIARPVVQ
                     GQFVFIAIAFGCLAYSFVNNDFSVLNVASHSNSELPLHFRFAATWGSHEGSLLLWIFL
                     LSGWSVAVSVFSKQLPDDMVARVLGVLGLVSVGFLLFLLFTSNPFDRLLPAALEGSDL
                     NPLLQDPGMVMHPPMLYMGYVGFSVAFAFAIAALLSGQLDAAWARWSRPWTTAAWVFL
                     TIGIMIGSWWAYYELGWGGWWFWDPVENASFMPWLVGTALIHSLAVTEKRGSFKSWTV
                     LLAITAFSLSLLGTFLVRSGVLTSVHAFATDPARGIFILVYLVVIVGFSLALFAWRAP
                     KVGLGGDFQLVSRETMLLTNNVLLLVAAGSVMLGTLYPLLMDALNLGKISVGPPYFET
                     IFVPLMAPAIFLIGLGPIARWKQASLPSLVTRLRWAFVVSMVSALTMPLLMEEWKPMV
                     SFGLLLAFWIITSIVVNIKHRIQNSGQGSVIARLSRQSSSYYGMHCAHLGIAAFVIGV
                     TMVNGYETEKDVRMELGNIVSIGGYTFKFNGTTNKPGPNYKATVGDIDVLKGDTVVRK
                     LYPEKRTYNASGMVMTEAAIDTGIIRDLYIALGEPLDNSAWVVRVYHKPFVVWIWLGC
                     ILMALGGILAISDHRYRFNILRKKQSEVNQTVEKEAASIPNSTGVAISSTIVSEGKSV
                     "
     gene            complement(8717..9163)
                     /gene="ccmE"
                     /locus_tag="PKZ49_05180"
     CDS             complement(8717..9163)
                     /gene="ccmE"
                     /locus_tag="PKZ49_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HND35990.1"
                     /translation="MKPRHKKMAIIAFSLTALTGAAILVLNVFQSNLVFFFSPTQVVA
                     KEAPIGKSFRIGGLVEEGSVKRQNDSTTVNFAITDTAETIKVVYTGILPDLFKEGKGV
                     VAQGKIANDGIFYADEVLAKHDENYMPPEAAVALEQAAKAQKSTQN"
     gene            complement(9147..9311)
                     /gene="ccmD"
                     /locus_tag="PKZ49_05185"
     CDS             complement(9147..9311)
                     /gene="ccmD"
                     /locus_tag="PKZ49_05185"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HND35991.1"
                     /translation="MGGYGLYVWGSYLATFICVIGEILMVKRSARTLRQQLSLTKESI
                     LRERKDEATS"
     gene            complement(9341..10081)
                     /gene="ccmC"
                     /locus_tag="PKZ49_05190"
     CDS             complement(9341..10081)
                     /gene="ccmC"
                     /locus_tag="PKZ49_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HND35992.1"
                     /translation="MSINWYKYAAPSSFYFLAGKLIPFFTVLAVIFLVAGLYIGFFVA
                     PTDFQQGEAYRIIFIHVPAAWMSMFLYVVMAFWAGIGLAFNTRLSSMFATAIAPTGAL
                     FTFIALWTGAWWGKPMWGTWWVWDARLTSELILLFLYIGFMSLQAAIDDPRRADKAGA
                     VIALVGVVNIPIIYFSVQWWNTLHQGASVSVMQSPKMATAMLVGMLVMVLAAWMYSIA
                     VILLRARNIILERESHTSWVAELRGTTR"
     gene            complement(10117..10776)
                     /gene="ccmB"
                     /locus_tag="PKZ49_05195"
     CDS             complement(10117..10776)
                     /gene="ccmB"
                     /locus_tag="PKZ49_05195"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HND35993.1"
                     /translation="MLMWIIRRDLMLAMRRQADVLITLFFFIIVVSLFPLGVGPEMNI
                     LRAIAPGVLWVAALLASMLSLGRLFSIDYMDGTLEQLLIAPSPLFLLVLGKMISHWLV
                     SGLPLVLMAPILGIQYDLSLEALGVLTLSLLIGTPILSLIGAIGAALTLGVRGGGVLV
                     SLLVLPLYIPILIFGAGAVEASAAGLGYDAHFSLLGAFLLVSLVFAPWAAAASLRISM
                     E"
     gene            complement(10783..11397)
                     /gene="ccmA"
                     /locus_tag="PKZ49_05200"
     CDS             complement(10783..11397)
                     /gene="ccmA"
                     /locus_tag="PKZ49_05200"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HND35994.1"
                     /translation="MLAATKLECTRGGHVLFTDLNFSVKSGELLQIHGPNGSGKTSLL
                     RMLCGLTQPSVGKITWNDSNIRSLGEQYYSCLTYLGHMIGIKDDLTAVENLSISCALA
                     GLEVDNRMALEALQQIGLKGRENLLARVLSQGQRRRVSLARLLLVKTKLWILDEPLTA
                     LDVAAIKLIKEKLEWFLAQGGMVILTTHQDIDISATAIHHLHLN"
     gene            complement(11451..12407)
                     /gene="truB"
                     /locus_tag="PKZ49_05205"
     CDS             complement(11451..12407)
                     /gene="truB"
                     /locus_tag="PKZ49_05205"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HND35995.1"
                     /translation="MDNLLVNPHSSPKSWVKRKINGVLLLNKPKHISSNRALQVVKRF
                     YRASKAGHTGTLDPMATGLLTICFGEATKFSSMLLNADKTYLATLRLGYISTTGDAEG
                     EIRSVNDLYKSKVELIPFQVQEILNNFLGKTNQTAPLFSALKLNGKPLYHYARANIVV
                     ERKVREVNIYDINLLKCENDVLTVRVRCSSGTYIRTLAEDIGKAMGWGGAYLTDLCRS
                     QVGLFCLDQAYGLDQFQDDVNARHDSLLYPVDSLLQGYHSVVLSEEQVNLLFNGQVVS
                     KENEQELPMGNKVKLYDLRGNFLGIGETIQANQIRPKRLVSH"
     gene            complement(12430..12795)
                     /gene="rbfA"
                     /locus_tag="PKZ49_05210"
     CDS             complement(12430..12795)
                     /gene="rbfA"
                     /locus_tag="PKZ49_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HND35996.1"
                     /translation="MGTLSSKGHARALRLADQIQRELADLIHHDLKDPRIGMITITDV
                     EVTRDYSHAKVFYTSLNDNADHSEIEAGLQHANGYLRKQLASRIKLRVFPQLHFVYDE
                     SIERGAYLSHLIDVANGRI"
     gene            complement(12799..>14190)
                     /gene="infB"
                     /locus_tag="PKZ49_05215"
     CDS             complement(12799..>14190)
                     /gene="infB"
                     /locus_tag="PKZ49_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HND35997.1"
                     /translation="TQHIGAYHVETAHGMITFLDTPGHEAFTAMRARGAKITDIVVLV
                     VAADDGVMPQTIEAIHHAKAAKIPIVVAVNKMDKPEANFERIKQELVTHGVVAEDWGG
                     EVIFVGVSAKTGLGIDELLESILLQAEVLELKAVKAAPAKGVVIESRLDKGRGPVATI
                     LIQSGTLKRGDVLLTGAAFGKVRAMLDENGNPIESASMSIPVEIQGLSDVAVAGETVL
                     ALEDERRAREIALFRQGKFRDVRLDQRQVAKMEEVFNQKDDIKILNLIIKADAQGSAE
                     ALAYALQKLATDEVKINIIHSGVGAIIESDINLALASKAIIIGFNSRADAGARKLIVT
                     TGVDIHYYNVIYDAVDEIKKALSGMLLPERKENITGLIDIREIFRISKIGVIAGCYVL
                     EGFVKRDSMVRVLRDGVVIHSCALDSLKRFKDDVKEVKAGFECGLSLKNYNDIQVGDQ
                     LEVYEVVEVMRTL"
BASE COUNT         4361 a         3332 c         2360 g         4137 t
ORIGIN      
        1 atacacttta atcactagcc tgtccacgaa taacctttct agcactaggg aatgcgccct
       61 ttattcctat cattgatatc aatttatcat cctatcaaga aaccatttac ctttaaaaat
      121 aagggtaata tttaaggtat atagctatca tctcgaacgg tgtagtaaag ccttttttgc
      181 agcctcatat tcttcttgag taattgcatt ttctgccagt gcacgctgca gatcttgcag
      241 ttcctgccct gtcgttatcg tggaagttcc ttgcactttt acagatgcat ctcctccaaa
      301 agcgcaactg gtaattagcg taattaatgc caaaactaaa actgtgaacc ttattgccat
      361 cttgaatttt ctcccaattt ttagcgaata ctatcaatta gtcgagcttt ttggcgctga
      421 aattccttct cagtcaatgc gcctgaatcg taagcttttt ttagatcgat aaactgttga
      481 ccaatcgaaa cattcaaatt gggctgttgt acaacttcag tgctactaca tccgcttatc
      541 actagcaaaa aacacatatt aactgcagtg attcttaact tactcactat ttctctccgt
      601 ttaataatga tagataaacc ctaatttaga ccctttatca tttagccaaa cgattgcaac
      661 cttccttctc atagtttaac taatatggtc tagactaata agatcttgtc tgctagctag
      721 gccttaatat tatgagctta gtgtgccgtg gtctccagat ccaatctaat tttttctaat
      781 cgctcatcat tcggaagtac acttccagct ctttccaaat agagctgtgc atcttccaac
      841 ctactttcga gtcttgcttt agctatgaac tgaatatagc gatcgacgat tctccttaat
      901 cctgcctggg cgatatcatt atgtgcatca atagcaagca ccatctgata atgcttaaac
      961 gcattatctt tagcaggcgt tgtaaaacgc gaagctctca tcgctttctc agcagcgtct
     1021 aaatgcatct tgataacatt cttatcaatc ggttcttcta caatcatttg tgcagatgtg
     1081 ttcactgata aatcagccat ggttgattgc tgatttcctt tcaattctga ttcaattgtt
     1141 gaatgctcta ctttatgatc ttcacgtact aaaggaatat ctggtaatgc ggatacattt
     1201 tcagaagatg ttttactgaa tggatcttca actaaatcct taatgtggtg ttcactcgac
     1261 aatatcggtt cgctaatttc aacaagatca gtccctttat ttacatcgac taaatcggat
     1321 ttcttttccc caaaccacaa tccaaccata ataaatgctg caatactaac tcctatcgcc
     1381 aaccccaacg gatttttagt aattttatgc ttaccaaaat catcaacgac ttctgcacca
     1441 gtaatatttg attgagcctg atctttaccc gttacattat cgattaacgc cattaactcc
     1501 tccactgatt gaggtcgatc attgcaagga agcttcagca tccaatcaac tgctttagtg
     1561 attgaagggc ttaatgtcat acctgataat tctgaaagtg atatcaaagg atcagattca
     1621 cctcgtttaa ttgcatttaa ccgattttgt gcggataccg gctccttatg ggtaatacac
     1681 tgatatagcg tcgcgccgag tgaataaaaa tcggttgcgg gcttaacttc cgttgtagat
     1741 tcatattgtt ctgagggcga gtaaactgta atcaaggtag aagtatcttc acccaatcgc
     1801 cttgcgagta tccaacgcgc agatgaaaaa cccgtcaaga taggctcgcc atcttttttc
     1861 aagaaaatcg attcgggctg gatcccctcg tgaataaaat tatatccatg cagttctttt
     1921 aaggcattta agattgatat cagtataaat ttgatctctg cctcttcaaa aattgttgat
     1981 ggctctagta tgcgagaaag tggtttgcct tcattacgat gtgttatcaa ataagctgtc
     2041 ccgttatgct ccaagatatt ttcagaggcg gctatattcg ggtgttccac gtgagtaaga
     2101 acatttcctt gatctagaaa tgccctcaaa cccaagtcat aatcttcctt attcgatgga
     2161 tttttgcatt caacaacttg gctaccgcta gcacgtaatg caagcttctg aggaaaatat
     2221 tcaagtatct caacttgttc ttttagatga tgattccaag cacgataagt aatatcaaat
     2281 accgtagttt ctttcgtatc ttgaatttca tagacaccaa tatgtgtacc ttttggcaga
     2341 gtctggtcgt gagtatatgt catgatattg ttgtcctttt tcctatacct tatattaacg
     2401 tgttgcctta aatattaaat catttagcga gttgaattca tctaggggaa atggctgatt
     2461 acgtatctca gtcttagatt ttcaatgata cataattcca ttgaccacta tctctttgat
     2521 gaaaataacc tttcccacaa actttaccat gatgcgctct atcgtaatta aatcttcatc
     2581 catatctctc attatccaac ttaaaatcga tcaaacaaaa tgattgaacc agcagtattg
     2641 cgataaccaa ttatcttaca aatagtaccc agttagctct gagcagaaat cgtttagaat
     2701 cctgactaaa ccgcgatact tcgatatact atataaaata catttaatta aaaattgata
     2761 gtatgaaacc tcctgtttgc atcacagtca caggcgcagc tgggcaaatt ggctacagct
     2821 taatatttcg gatagcggct ggggacatgc ttggtaatga tcaacccatc attcttcgtt
     2881 tactggatgt tactgaatcg caaggttttc ttaatggtat catcatggag cttgttgatt
     2941 gtgcctttcc gctcttaata gatgtcatta aaactgatga tccactcgtc gctttcgata
     3001 aggctaatat tgcgatccta gtcggcgctc gaccacgaac aaaaggaatg gagcgtaagg
     3061 acctgctagc tgctaatagc gttatattca gtacccaagg taaagcactc aatgaagttg
     3121 ctgatccaaa cgtaaaagtg ctcgttgttg gcaatccagc caacacgaat gcatatattg
     3181 cgatgaagaa tgcacctaaa ctaccaataa ataacttttc agcaatgctc cgattggatc
     3241 acaatcgagc attgtcccag gtagctatga aaattaatca acccgttaca gaaattagaa
     3301 aaatgattat ctggggcaat cattcttcaa ctcaatttcc tgatattgat cacacagagg
     3361 ttagaggcga gaaaataatc aaccttatta gtgattacaa gtgggttgaa aattacttcc
     3421 ttccaactgt gcaaaaccgg ggtgcagcta ttatcgaagc gagaggtggg ttatcgagcg
     3481 ctgcaagcgc tgccaatgca attattaatc atatgcatga ttggttgatt ggaactcgag
     3541 aaaatgactg ggtaacaatg gggataccct ctgatggtag ttatggcatc ccagaggaaa
     3601 ttatttatgg attccctgtt acatgtcaga atggcactta caggattgtt caaggacttg
     3661 aaatcagtag tcgatctaag gctaaaatgc ttgaaaccta ccaagaactc ctcgaagaaa
     3721 aagattcaat caagcattta ctttcaaacc gatgaaactt caattcaaag tgttttaata
     3781 aatgatagaa cctcatctac atgaccaggc actttcacac cacgccactc tcttattagc
     3841 ttgccctgtt catcgataac aaaagtactc cgttcaatac cacgaacttg tttgccatac
     3901 atatttttca atttcatgac ttcaaagcga ctacaaaccg tttcgtttga atcacttaat
     3961 aaatcaaatg gcaatcctag tttttctcta aaattttcgt gtgatttaat gctatctctt
     4021 gaaattccaa caacttcaca attaagtgct ttaaattgtt catataaatg gctaaattgc
     4081 tgactttcgg tggtacaacc cggcgtgtca tcttttggat aaaaatagat tatcaacttc
     4141 ttaccacgat agtcagttaa cctaaaaggt ttaccttcag tcgattcaag ttcaaagtca
     4201 ggtataggat tagctaacat ctgagtatca attaataaag aaatttatgt ttagggtatg
     4261 atttgatcaa ttgtaatcaa aacatcttta tcgcccaatt taactggttg gctaaatcct
     4321 tctaagtctc cacttgtcgg aactgcctga cctgatttac ttaccctagc cccaataact
     4381 acttcaggaa aactagacat tttcatggta ggagtcatag ccatatcatc gtcaagtgaa
     4441 aaagttgctg gtaaatcctt agcacttaat ctgaggattg ctaaaggcat tttagatcct
     4501 gtttttgcac gagcaaaaac aaacaaaaca tccgtagcgg atactttact tgccagatca
     4561 ggactaatga tcacttttcc agataccttt gttgatgtaa tcgaatttgc tgctatctta
     4621 acttcatctg aagacgtagc atcatgctgt aaatcacttg agacctttga attctgagct
     4681 aattgaactg ggtgatcttc ttttcctttt acagcaagtg tcttggcttg ctcgatgcta
     4741 ctagtcactg atgctgctaa ttgagaatct tcaggaataa gcgccaccag cctctgccag
     4801 taatttgctg cttgttcata gttttctttc tcaaattcaa ctgttccggc cagagcaagt
     4861 gctttcgggt attctggatc aatgctcagt gcatgagcaa tcaactcagc tggtctacca
     4921 atcaagcttc cattattcgt cattgccaac acatctgcat agtcacttaa taattgcgca
     4981 ttgtcgggaa tcatctcgac taacttagca taagtagcac tcgcttgttg atattgaccc
     5041 attatggcgt aggttctgcc taacataacc catccttcga tatcttccgg attatcttga
     5101 agacgcgcta ctagattatc aacaactgat ttaatctcag catgcccttg tggcactccg
     5161 gccatatttt cagaatgaaa ctgtgttgca tttgcaagct gcgactgtgg caacaatccc
     5221 cgcgtatcac cgatcgccat atacaacgat atagctgtta ggggaatagc aagcataatc
     5281 acaaccgcag ttactaaacc acgcgcttta ctgggtaatg catcggtgcc ggactcagtg
     5341 cccgatgcat tgacatctag caacattctt ttttgtaatt cttgtttact cttttcgtac
     5401 tgttccttag ataaaacatc gttactcaaa tctgcatcta attcagctat ctgatcacga
     5461 taaatcgcaa tattagctgt ttgacgctct agtgctgtta tttttccctg ccgactcttg
     5521 atcaaggttg gcaaaacgaa catcagcgca gtaacaataa aagcacctgc aacaacccaa
     5581 aatgtagtca tcctttttta ttccttctca atttttctaa tcaaggtttc tgcttctagc
     5641 tgctgactct cagatagttg ctgcggttct tcaaatacta gcgatcgacg acgtcttaaa
     5701 tataaaacta aagcaaggat tgcccctaag aataataaga tggggccaag ccatagcaat
     5761 attgtcgttg gcttaaacgg tggattgtaa agaacaaaat caccgtatcg cgcaactaga
     5821 aaatcgatta tttcttcatc gcttttatta gctttcattt gttctcgaat ttctcgcctc
     5881 agatcattcg caaaatcagc ccgtgagcca gccagtgatt cattttggca aactaaacag
     5941 cgtaagttct ccgaaagctc aatcaaacgt ttttctaaaa tggggtcttc agctactgga
     6001 attgcttcat tagcaaaact tatttgtaca aaaaatacta atgtaaatac aagcgacatc
     6061 cgccaacttg acagaaaata actgtttgaa taattcatta atctcaacct tgcaattctt
     6121 ttatcagagg taggatttta tcctccaatg atttcaccgt gacgggtccg atatgtttat
     6181 aacgaatgat tcctttctta tcaataacat aagtctcagg aactccatat accccataat
     6241 caatccctac ctttccatca gcgtcaataa tacttaccgc atagggatca ccatattgct
     6301 tcaaccattg aattgcagta cttcgctcat ctttataatt caatccataa attgggacta
     6361 tgccaagttt agcaagctga actaacaatg gatgctcatc tcgacaagct acacaccatg
     6421 aggcccaaac atttagcatc cagaccttac ccatattgtc ttgcgatgct aatgtcttcg
     6481 aaggatcatg taattgctgt aattcaaaga caggtgcagg tttatcaatg agcggagaag
     6541 gaacttgacg cggattcagt gttaatccta tccctaaaaa aaccactaat acaataaata
     6601 ctgctaatgg caatagaaag cgcatcatac gcttttacct tctgaaacga ttgtcgagct
     6661 aattgcaacg ccagtggaat ttggaatcga tgccgcttcc ttttcaacag tctgattaac
     6721 ctcagattgt ttttttctaa gaatattaaa tcgataacgg tgatctgaaa tagccagaat
     6781 tcctcccaat gccatcaaaa tacaaccgag ccatatccaa acaacaaagg gcttatgata
     6841 cacacggaca acccaagcac tgttatccaa cggctctccc aatgcaatat acaaatctct
     6901 tataatacca gtatctatcg cagcttctgt cataaccata ccggatgcat tgtaagttct
     6961 tttctcagga tataatttgc gcaccacggt atcacctttc agcacatcga tatcacccac
     7021 tgtcgctttg tagtttggtc ctggcttatt tgtagttcca ttaaacttaa aggtatagcc
     7081 gccaattgat acaatatttc caagctccat ccgaacatct ttctcggttt cataaccatt
     7141 aaccatcgtt acaccaatta caaaagctgc tatacctaag tgcgcgcaat gcattccata
     7201 gtaactagag gattggcgac tcaatctcgc tatgacgctc ccctgcccac tattttggat
     7261 acgatgttta atattgacaa caatacttgt gataatccag aacgccaaaa gtagaccaaa
     7321 actaaccatg ggcttccatt cttccatcaa taaaggcatg gtcagagcgc ttaccatact
     7381 aaccacgaat gcccagcgta aacgagtgac taatgacggt aaacttgctt gcttccaccg
     7441 agcaattggt ccaagaccaa tcaagaaaat agctggtgcc atcaaaggaa caaatattgt
     7501 ttcaaaatag ggagggccaa cagatatttt gcctaaattc aacgcatcca ttaataaagg
     7561 atagagtgtg ccaagcatga cactccctgc agccacgagc aataagacat tattagtcaa
     7621 taacatcgtt tcgcgtgaca ccaattgaaa atcaccgcct aagcctactt taggcgcacg
     7681 ccacgcgaat aacgctaatg aaaaacccac aatcactact agataaacta aaatgaaaat
     7741 accacgggct ggatcagttg cgaatgcatg aactgaagtc aaaacacctg aacgcaccaa
     7801 gaatgtaccc aataagctta atgagaatgc tgtaatcgcc agtaaaaccg tccagctttt
     7861 gaaactacca cgcttctcag ttacagccaa agaatgaatc agtgcggttc caactaacca
     7921 tggcataaaa gatgcattct cgacaggatc ccaaaaccac catccacccc aacctaattc
     7981 ataataggcc caccaactac cgatcatgat accaatcgtt aagaataccc atgctgcagt
     8041 ggtccaagga cgtgaccacc ttgcccatgc tgcatcgagc tgtccactca ataacgcagc
     8101 aattgcaaag gcaaatgcaa ccgaaaaccc aacatatccc atataaagca ttggaggatg
     8161 catgaccatt cctggatctt gtaataatgg attcaggtca cttccttcaa gcgcagcggg
     8221 taataaacga tcaaatgggt tagaggtaaa taataaaaat aacaaaaatc caacgctaac
     8281 caatcccaaa acacctaaga ctctcgcaac catatcatca ggcaattgct tactaaatac
     8341 gctgacagca acagaccaac ctgataacaa gaatatccat agtaataacg aaccctcatg
     8401 tgatccccaa gttgcagcaa atctgaaatg aagcggtaat tcagaattgg aatgagatgc
     8461 gacattgagt actgaaaaat cattattaac aaaagaataa gctaagcacc caaatgcgat
     8521 tgcaataaat acaaactgcc cttgtactac tggccgcgct attgctatcc aagcaggaat
     8581 accacgagcc gccccgatta atgggagcgt gccctgaatc aatgctaata acattgcaag
     8641 aatcaaagca aaattaccga gttctgggac cataattatt aagtattaaa gattacaatg
     8701 aaaaaagatt attctattaa ttttgagttg atttctgggc tttagctgct tgttcaagtg
     8761 caacagctgc ttctggaggc atataatttt catcatgttt cgcaagaacc tcgtcagcat
     8821 aaaaaatgcc atcatttgca attttcccct gcgccacaac ccctttccct tccttaaata
     8881 aatcaggaag tatcccggta tagactactt ttatggtttc tgccgtatcg gtaatcgcaa
     8941 aattaacggt tgtggaatca ttctggcgtt taacgctacc ttcttccacc aacccaccaa
     9001 ttcggaaact tttcccaatt ggcgcttcct ttgctacaac ctgagttgga ctaaagaaaa
     9061 acacaagatt actttggaac acattcaaca ccaaaattgc tgcacccgtt aatgcggtaa
     9121 ggctaaaggc tattattgcc atctttttat gacgtggctt catcttttct ctctcttaaa
     9181 attgactctt ttgtgagact cagttgctgc cttaaagttc tagcgcttct tttaaccatc
     9241 agaatctcac ctataacaca tataaatgta gctagataag acccccaaac atagaggcca
     9301 tatcctccca tattaaaaaa atcggaccag ctatcccaga tcatcgcgtt gttcctctta
     9361 attcagctac ccagcttgta tgactctcac gttccaatat aatgttccgt gctctaagca
     9421 agattaccgc tatagaatac atccaagcag ccagcaccat cactaacatt ccaaccaaca
     9481 tcgcagttgc cattttaggt gactgcatca cgctaaccga tgctccttga tgcaaagtat
     9541 tccaccactg aaccgaaaaa tagataattg gaatattgac aaccccgacc aacgctatta
     9601 cagcaccagc tttatcagct cttctcggat catcgatagc tgcttgtagt gacataaatc
     9661 caatatataa aaacaataat atcaattcag aagtcaatct agcgtcccaa acccaccaag
     9721 ttccccacat cggcttcccc caccacgcac ctgtccataa agcaataaat gtaaataaag
     9781 ccccagtcgg tgctatggct gtagcaaaca tagatgaaag tcgagtatta aacgccaatc
     9841 ctatccctgc ccagaaagcc ataacaacgt ataaaaacat cgacatccag gccgctggaa
     9901 catgaataaa gataatgcga tatgcttctc cctgctggaa atcagttggc gccacaaaaa
     9961 aaccaatgta tagccctgca acaagaaaga ttactgctaa aacagtaaaa aaaggaatta
    10021 attttccggc aaggaaataa aaactcgagg gtgctgcata cttataccaa tttattgaca
    10081 tactaactaa ttttataaat ttactaaaat aatattctac tccatcgaaa tccttagcga
    10141 tgcagcggct gcccaaggag caaaaacgag tgaaaccaat agaaaagcgc caagcaatga
    10201 aaaatgtgca tcatacccta aacctgcagc gcttgcttca actgcaccgg ccccaaaaat
    10261 taatatgggg atataaagtg gtaatactaa taacgaaact aatacccctc ctcccctaac
    10321 tcccaatgtc agcgctgcac cgattgcgcc aatcaaactc agtattgggg tccctatcaa
    10381 taatgatagc gttagaactc ccaaggcttc cagggacaaa tcgtattgaa taccaagaat
    10441 aggtgccatt aatactaaag gcaaaccgga aactagccaa tgtgaaatca tctttcccaa
    10501 taccaaaaga aacaaaggcg atggtgcaat caataattgc tctaatgtac catccatata
    10561 atcgatggaa aacaatcgac ccagtgacaa catcgaagct agtagtgcag ctacccataa
    10621 tacacctggc gctatggccc tcagaatatt catttctgga ccaacaccca gtggaaatag
    10681 gctaactaca ataataaaaa aaaacaatgt gatcaacaca tcagcctggc gacgcatcgc
    10741 tagcatcaag tctcttctga ttatccacat caacatttca tttcaattca agtgtaggtg
    10801 atgaatcgct gtcgctgaaa tatcgatgtc ttgatgtgtc gtcagtatta ccatcccacc
    10861 ttgagcaaga aaccattcta acttttcttt gattaatttt atagctgcca catctagcgc
    10921 agtaagtggc tcatctaaaa tccacaattt tgttttaact agcaataatc tcgccagaga
    10981 tactcttctt ctttgtcctt gtgacaacac tcgggccagc aagttttctc gcccctttaa
    11041 accaatctgt tgtagcgcct caagcgccat tctgttatca acttctaaac cagccagtgc
    11101 gcaagaaatt gataaattct caactgccgt tagatcatcc tttattccga tcatatggcc
    11161 taaataagta agacagctat agtactgttc gccaagtgat cggatatttg aatcattcca
    11221 agtaatcttc ccgacgcttg gctgtgtcaa tccacataac attctcaaca aacttgtctt
    11281 accgctacca ttagggccgt gaatttgtag caattcacct gatttcactg aaaaattaag
    11341 atcggtgaat aaaacgtgtc ctccccgtgt acactctaat ttggtagctg ctagcattta
    11401 ttatttataa gactaagttt caattcaaaa tctcaacgca tcgttaatgt tcaatgtgac
    11461 actaatcttt tgggccttat ttgattggct tgaatcgttt ctccaattcc cagaaaattc
    11521 cctcgtaagt catataactt aactttattg cccatcggta actcttgctc attttcttta
    11581 ctaacgactt ggccattaaa caacaaatta acctgttctt cacttaacac tactgaatga
    11641 tatccttgca aaagagaatc aactggatag agaagtgaat catgtcgtgc atttacatca
    11701 tcttgaaatt gatcaagccc ataagcttga tctaagcaaa acaaccctac ttggcttcta
    11761 caaagatcag ttaaataagc gcccccccaa cccattgcct tacctatatc ctcagctaag
    11821 gtacgaatat aggtcccaga actacatcta actcgaaccg tcaacacatc attttcgcat
    11881 tttaacaaat ttatatcgta aatatttact tcacgcactt ttcgctccac aacgatattt
    11941 gcacgcgcat aatgatataa aggtttgcca tttagcttta gcgcactaaa aagaggtgca
    12001 gtctgattcg ttttgccaag aaagttattt agaatttctt gaacttggaa aggaatcaac
    12061 tctaccttac ttttatacag atcattgact gacctaattt caccctctgc atcacctgtc
    12121 gtactaatat atccaagtct caatgtggca agataggttt tatctgcatt caacaacatc
    12181 gatgaaaact ttgttgcttc accaaaacat atcgtcaaca aacctgtcgc cattggatca
    12241 agcgttcctg tatgccctgc tttagaagct cgataaaaac gcttcactac ttgtagtgca
    12301 cgattagaac taatatgttt tggcttgttc agtagcaaga ccccgttaat cttacgctta
    12361 acccaagatt ttggagaaga atggggatta accagcaaat tatccacaat tcaattttga
    12421 aaatctgatt cagattctcc cattcgccac atcgatcaaa tgcgagagat atgcacctcg
    12481 ttcaattgac tcgtcgtaaa caaaatgtaa ttgtggaaaa actcttaact taattctact
    12541 ggctaattgt tttcttaagt aaccatttgc atgctgcaat cctgcctcaa tttcgctatg
    12601 gtcagcatta tcatttaatg aagtataaaa aacctttgca tgggaatagt ctctcgtcac
    12661 ttcgacatct gtgatcgtta tcattccgat gcgaggatct tttaaatcat gatgaatcaa
    12721 atcagccaat tcgcgctgaa tctgatcagc tagccgtaat gcacgtgcat gaccttttga
    12781 cgatagcgta cccatggttt ataaagtccg catgacctca acaacttcat agacttcaag
    12841 ctgatcaccc acttgaatat cattataatt cttgagtgat agcccacatt caaatccagc
    12901 ttttacttcc ttcacgtcat ctttaaaccg tttcaaagaa tcaagcgcgc agctatggat
    12961 aacaacgcca tcccgcaata ctcgaaccat agaatctcgc ttaacgaaac cttcaagaac
    13021 atagcaccca gcaattacac caatttttga gattcgaaat atctctctga tatcaatcag
    13081 cccagtaata ttttctttac gttcaggcag caacattccc gaaagtgcct ttttgatctc
    13141 atcgaccgca tcgtagatca cgttatagta atgaatatca acgccggtag taacaatcag
    13201 tttcctagcc ccagcatcag ccctgctgtt aaacccaatg ataatggctt tcgaagcaag
    13261 cgccaaattg atatctgact caataattgc acctacacca ctatgaatga tattaatttt
    13321 cacttcatcc gtcgcaagct tctgcaatgc gtaagccaaa gcttcagctg acccctgcgc
    13381 atcagcctta atgatcagat taagtatttt aatatcatct ttctgattaa atacctcctc
    13441 catcttagcg acttggcgct gatcgagtct cacatctcta aatttaccct gacggaaaag
    13501 cgcaatctcc cttgcacgac gctcatcctc caacgcgaga accgtttcac cggcaactgc
    13561 cacatccgac aaaccttgaa tttctacagg aatagacata ctggcactct caatgggatt
    13621 accattttca tccagcatgg ctctaacttt tccaaatgct gcaccagtca gcaatacatc
    13681 accgcgcttt aacgttcccg actgtattaa gattgtagcc actggcccac gccctttatc
    13741 tagccgcgat tcaatgacga ccccctttgc aggcgctgct ttgacagctt taagttcaag
    13801 aacttccgct tgcaataaaa tactttctag taattcatca attcccagac ctgtttttgc
    13861 agagacgccg acaaaaatga cctctccacc ccaatcctca gcaaccacac cgtgcgtgac
    13921 taactcctgt ttaatacgct caaaattggc ttctggttta tccatcttat ttacagcaac
    13981 aactatcggt attttcgctg ccttagcatg gtgaatagcc tcaatcgttt gtggcataac
    14041 tccatcatct gcagcaacca ctaacacaac gatatctgta atcttagcgc cacgcgccct
    14101 catcgccgtg aaagcttcat gccctggcgt atctagaaat gtaatcattc catgcgctgt
    14161 ctcaacatga taagccccaa tgtgctgggt
//
LOCUS       DAOQKQ010000056        13941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_2054_length_13941_cov_5.468890, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000056 DAOQKQ010000000
VERSION     DAOQKQ010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 13941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13941
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2054_length_13941_cov_5.468890"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            465..854
                     /gene="sdhC"
                     /locus_tag="PKZ49_05220"
     CDS             465..854
                     /gene="sdhC"
                     /locus_tag="PKZ49_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HND35998.1"
                     /translation="METKFQSRRPKYLNLVKIKQPLPAIVSILHRISGVLLFFPGLPL
                     LLYCLQQVLESPESYAQFQSVIANPIIKWGFIFFLWALVHHFCAGIRHLALDLHLGST
                     LEGARLGSKLVVAFSIGITVLLGVLLW"
     gene            848..1198
                     /gene="sdhD"
                     /locus_tag="PKZ49_05225"
     CDS             848..1198
                     /gene="sdhD"
                     /locus_tag="PKZ49_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HND35999.1"
                     /translation="MVARTKRVLTGAHYGLIDWLVQRVTAVLMVLYLLLLAWVFTDQA
                     SDYAGLKSIFANQWMRIFSLLVFIGLCWHAWVGVRNVLMDYVKITSIRLSVQVLVITA
                     LLFYLIWFVDVLWR"
     gene            1200..2963
                     /gene="sdhA"
                     /locus_tag="PKZ49_05230"
     CDS             1200..2963
                     /gene="sdhA"
                     /locus_tag="PKZ49_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HND36000.1"
                     /translation="MTIRKRKFDVVIVGAGGAGMRAALQLSEAGLTVAVLSKVFPTRS
                     HTVAAQGGIAASLGNVTEDNWHWHMYDTVKGSDYLGDQDAIEFMCRQAPEVVYELEHF
                     GMPFDRLDNGKIYQRPFGGQSQNYGGAQATRSCAVADRTGHAMLHTLYQRNLYANTQF
                     FVEWMGLDLLRDQQGDVLGVTALEMETGEVMILQAKVTLLATGGAGRIFQASTNAFIN
                     TGDGLGMAARAGIPLEDMEFWQFHPTGVHGVGVLISEAVRGEGGYLLNKNGERFMERY
                     APNAKDLASRDVVSRALTTEIKEGRGCGKDGDYLLLKLDHLGPEIIKSRLPGIREIAI
                     KFAHADPIEEPIPVVPTAHYMMGGIPTNFYGQVVAPYKTGPEEVVSGLYAVGECACVS
                     VHGANRLGTNSLLDIVVFGRAAGNKIIEELKHNPHHKSLPEDIADISLARLNRLDTQS
                     NGEKVADVGDALRRTMQTYCGVFRFPDMLKEGVSKINEVAQRVARTEINDKSKIFNTA
                     RIEALELDNLIEAAIATMVSAEARHESRGAHTRDDFPERNDEKWLKHTLYFKEGQRLD
                     YKPVRLKPLTVDSFPPKARVY"
     gene            3054..4721
                     /locus_tag="PKZ49_05235"
     CDS             3054..4721
                     /locus_tag="PKZ49_05235"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HND36001.1"
                     /translation="MTKYVFVTGGVVSSLGKGIAAASLAALLETRGIKVTMLKLDPYI
                     NVDPGTMNPFQHGEVFVTDDGAETDLDLGHYERFISTKMAKRNNFTTGQIYESVIRKE
                     RRGDYLGGTVQVIPHITDEIKLFIRNGVNDAQVAIVEIGGTVGDIESLPFLEAIRQMA
                     VQHPRDDTCFIHLTLLPYIGSAGELKTKPTQHSVKELREIGIQPDVLLCRSDRMLPQE
                     ERRKIALFTNVKEEAVISAIDVDCIYKIPALLHEQMLDEIICHKLNILAKPANLSVWK
                     NLVYALEHPTKTVNIALVGKYVDLTESYKSLSEALIHAGIHTHCKINICYLDSELIEK
                     EGTDCLSDKDAILVPGGFGKRGVEGKILAIHYARTHKIPYLGICLGMQLAVIEYARNE
                     LHLENAHSTEFNPNTPYPVLGLITEWRTREGQIEKRSEATDLGGTMRLGGQLCFLNED
                     SLAKKIYGSDKITERHRHRYEVNASFIPSLEKAGLKISGVSAEEKLCEMVELSQDEHP
                     WFVACQFHPEFTSTPQKAHPLFKAYINAAISYANQRTDNSLEQKEIA"
     gene            4784..6067
                     /gene="eno"
                     /locus_tag="PKZ49_05240"
     CDS             4784..6067
                     /gene="eno"
                     /locus_tag="PKZ49_05240"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HND36002.1"
                     /translation="MSAIVDVIAREILDSRGNPTIEADVLLESGALGRASVPSGASVG
                     TKEAIELRDEDEERYFGKGVTKAVDNINTEISETIMGLDASEQAFIDQALIDLDGSEN
                     KSRMGANAILAVSLAVAKAAAEESSLPLYRYLGGVRAISMPVPMMNLINGGAHANNNL
                     NMQEFMVIPHGFDSFREALRCGAEIFHTLKKLISKKNMPTTVGDEGGFAPNLSGNEEG
                     LQLIVQAIDQAGYQPGTQVAIGIDCASSEFYQDGKYHLKSDGLSLTSAQFVDYLATWV
                     DKYPIISIEDGMSEHDWDGWKLLTERLGKTVQLVGDDVFVTNSRILKEGIQRGIANSI
                     LIKVNQIGTLTETLTAVEIAKCAGYTAVISHRSGETEDTTIADIAVATNALQIKTGSL
                     SRSDRLAKYNQLLRIEEDLGDNAIYAGRSAFYQLT"
     gene            6067..6372
                     /gene="ftsB"
                     /locus_tag="PKZ49_05245"
     CDS             6067..6372
                     /gene="ftsB"
                     /locus_tag="PKZ49_05245"
                     /inference="COORDINATES: protein motif:HMM:NF002058.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HND36003.1"
                     /translation="MKTVALILVTLIALVQYSLWYGKGGWLEVFELEKQLQDQHQLNQ
                     KSQNQNTILEAEIIDLKQGFDAIEELARNELGMIRKDEVFFQVQQPTEVPSVTKSKK"
     gene            complement(6437..6904)
                     /locus_tag="PKZ49_05250"
     CDS             complement(6437..6904)
                     /locus_tag="PKZ49_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HND36004.1"
                     /translation="MTAAFSFTTEWRFDAPHEMVCDAIIQCLDWPQWWLGAEQVEKLV
                     TGDSDGVGSVHRFTWKGKIPYHFTFTIRVTRYVPGVLLEGVAEGEVVGTGRWDFIRAN
                     DITIVRYAWTVRTNRLWMNLLAPIARPIFKWSHDQVMRQGELGLAHWLRMQSK"
     gene            complement(6910..7545)
                     /locus_tag="PKZ49_05255"
     CDS             complement(6910..7545)
                     /locus_tag="PKZ49_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36005.1"
                     /translation="MLAGLMLTMALMAPELPNPIEKAVDYYQKVDSYQTIIKSYVGSK
                     SDSPDIVRYYYNKSGAVRMEVVEPFNGAVLIYNPVTELVKLWLFGYGSVPSFSLSPEN
                     KWIQSSSGQRVDRSDIGALYEDVILLQRQGKTTVVGTETVEKQAALYVIVEGNPGVAI
                     EGVSRFDLWFDVQTSLPLKVISYKADGSQIERIEMSEYQVNPAFPKGFFDH"
     gene            complement(8157..8588)
                     /locus_tag="PKZ49_05260"
     CDS             complement(8157..8588)
                     /locus_tag="PKZ49_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HND36006.1"
                     /translation="MKFHIFILGLFILALTACSTTRPILYPNTHYKTVGQHAAEQDIV
                     ACRQLAEEAGAQEGNASQVGNVATSTAMGAGMGAASGAVGGAISGAAGTGSLIGAASG
                     AALGLIRGLFGLARPTQPNQAYVNFVYRCLQDKGYEVTGWQ"
     gene            8912..10942
                     /locus_tag="PKZ49_05265"
     CDS             8912..10942
                     /locus_tag="PKZ49_05265"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HND36007.1"
                     /translation="MTVTLTNLDDNYIDGPGGNEIFAQLGNDTVNGADGSDTIWGEGG
                     NDSILGGNQNDTLYGGIGNDTLNGGSGVDRLEGGADNDTYIIDSNGIFLPLDTIVDSG
                     GIDTVLSSVTYTLGAAIENLTLTGAAATSGTGNVSANVITGNSANNTLNGLNGNDTLN
                     AGLGNDVLNGGDQNDTLNGDGGVLALSGGNDTLNGGNGNDTLSGGLGNDVLNGDVGDD
                     RLVGGSGNFFQPIDVDTLSGGIGNDTYVISENTDVITELSGEGTDTVESSITFNLGTS
                     GANVENLTLTGTAVINGTGNTLSNVINGNSKDNTLDGGAGNDTLKGNDGNDTLIGGPG
                     LTVVIGPFGPTFISDNDTLEGGKGNDTYIMDYTGDTITENLNEGIDLVKSSLSSYTLP
                     TNVENLTLTGVGNINGLGNSLDNVITGNAGINALSGDIGNDTLNGGGGNDVLDGGQGN
                     DTYIADGGDTISEAAGAGTDRVESSITFSLAAIANVENLTLTGAANINGTGNSLNNTL
                     VGNSGTNTLTGSGGNDVLIGGAGADILNGGNDDDTFWYDATDVSVAGNRISGGSGTDT
                     LTFMSSAVQNLNLTTLADDRITGIEQININGFNLFGPSPNNTLTLNVTDVFAISDTDT
                     LRVDGGVGDVVNSIGQGWANTGVVVIGTQNYDQYTFGTATLLVDTDIGGTIS"
     gene            11187..12437
                     /locus_tag="PKZ49_05270"
     CDS             11187..12437
                     /locus_tag="PKZ49_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HND36008.1"
                     /translation="MIEKELKLFGMAASQGRWLFVLIGLSINICLGSIYAYSVFRKPL
                     ETLWDIPSSQSGYPFMIFLAVFAITMPLAGSLMAHWGPRKTATLGGLLVGIGWMAASF
                     APDITTLTLLYGVIGGAGVGIVYGCPIAVVAKWFPQKSGLAIGLTVMGFGLSALITAP
                     LMKAMIIHPEIGTLNTFFYLGVSFLFIVSLLARCLRFPPDEWSSVNVITHSSTTTANP
                     TIELSRHDMIRTRHFYALWATYTIGCLAGLMAIGIASPVGIEVAKLEPTTAALAISLF
                     AIFNGLGRPLFGTIADKLGPKQTAMLSFAMIGGASLLLSGLEEGDTTLYLFAFCVLWL
                     CLGGWLALAPTSTGIFFGKKHYAQNYGVVYTAYGVGAILGTLISGNIKDITGSYLGAF
                     PIVAGLAVLGLLIAAVGLNSVKNK"
     gene            12476..13855
                     /locus_tag="PKZ49_05275"
     CDS             12476..13855
                     /locus_tag="PKZ49_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HND36009.1"
                     /translation="MFNQHHFWALICGPLLAGVVAASMHFSGWHVEACWTAAIVLLCV
                     IWWIFEPIPIPATSLIPITFFPLTGVLAKEEVAKAFGNDLILLMLGGFMISVAMAQSG
                     THRRLALGLVNFIGGESSRRIVFGFMCACAVLSMWISNTATALMMLPLALAIIDQSQD
                     KKIAFPLLLSIAYGCSIGGLGTPIGTPPNLIFMKEYQSFTGSTVSFSQWMIWGLPVVF
                     LMVPLAGLWLTRHLSYVGKLEMPQAGAWRPEERRVLIVFGITVLAWITRLEPFGGWST
                     WLELKNATDATVALIAVIVMFIIPNGRGEKLLNWEAAAKIPWGILLLFAAGIAIAQAF
                     IESGLSKFIAEQLAILSNLHPLVIIVSIALAVTFLTEATSNAATTILLMPILAPAALA
                     AGLDPALLMVPAAMSASCAFMLPVATPPNAIVFGTGKIPIAGMVREGLALNFIGVTVI
                     TTVCYFLVP"
BASE COUNT         3751 a         2794 c         3359 g         4037 t
ORIGIN      
        1 cgagatagtc aaggttattt tgactaacgg ttagcgcgat ggtgaaaaaa gcttaatcaa
       61 tgtggtgcta atttttcatt gtgaaattat agaatgctgc tattaaagat gattttgtta
      121 aattaacgat cttttggttt aattaccgtg gcttcaatgg acaaaaaata gcctataatt
      181 gccgcccatt tttttgttta cctttgccaa aacgcgctac ctcttatcca cgctgcagtt
      241 tttttcaacc gccttgctgt tgaatcgatt caattcttgt gtgatcgtgt ggtttcaatc
      301 atctttctgc gtagatgcac tgtgattgat aggcttggtt tagctaaaag tttgcttgtt
      361 tactataagt cgagattaaa gcgttgaatg aattgattta agggattaaa ctagcagtaa
      421 cctttttaat gatatcggaa ttcagtaggt cagtgattta aggtatggag actaaatttc
      481 aaagcagacg acctaagtat ttgaatctgg ttaaaatcaa gcaaccactc cctgctatcg
      541 tttcaatact gcatcggatc agcggtgtat tactgttctt tcctggttta cccttattgc
      601 tttattgcct gcagcaagta ttagaatcac ctgaaagtta cgctcagttt caatccgtga
      661 tcgccaatcc catcatcaaa tggggtttca ttttcttcct ttgggcactg gttcatcatt
      721 tttgtgcagg aattcgccac ttggcacttg atttgcattt ggggagcaca ttggaaggtg
      781 cacgcttagg gagtaagtta gttgtagctt ttagtattgg gataaccgtt ttattgggag
      841 tcttattatg gtagcccgca caaaacgggt tctgaccggc gcacattatg ggttaatcga
      901 ttggttagtt cagcgcgtta ccgcagtatt aatggtttta tatttactgt tgcttgcttg
      961 ggtatttacc gatcaagcaa gtgattatgc tgggctaaaa tcgatttttg ctaatcaatg
     1021 gatgaggata ttttctctac tggtgtttat tggtttgtgc tggcacgctt gggttggcgt
     1081 caggaatgta ttgatggatt atgtgaagat taccagtatt cgattgagcg tgcaggtttt
     1141 agtcatcacc gctttgttgt tttatctcat ctggtttgtt gatgtgctat ggagataatg
     1201 tgaccatccg caaaagaaaa ttcgatgtcg tgattgttgg ggcagggggt gctggaatgc
     1261 gtgctgcctt gcaattatct gaagccggtt tgacggttgc agtattatct aaggtttttc
     1321 cgacgcgctc tcatacagta gcagcacaag gtgggattgc agcatcattg ggtaacgtaa
     1381 ccgaagataa ctggcactgg catatgtacg ataccgtgaa aggttctgat tacttgggcg
     1441 atcaggatgc gattgaattt atgtgccgac aggcgcctga agtggtttat gagttagagc
     1501 attttggtat gccattcgat cgtctcgata atgggaagat ttaccagcgc ccttttggcg
     1561 gtcaatcgca aaattatgga ggagcacaag caacaagatc ttgcgcagtg gctgatcgca
     1621 cgggtcatgc aatgctacac acgctttatc agcgtaattt atatgccaat actcagtttt
     1681 ttgtcgaatg gatgggtctt gatctattac gtgatcaaca gggggatgta cttggtgtaa
     1741 cggcgctgga aatggaaacg ggggaagtca tgatcctcca agccaaggtt actttgttag
     1801 cgacgggtgg ggcagggcgt atctttcagg caagtaccaa tgcgttcatt aatactggcg
     1861 atggattagg catggcagcg cgtgcaggta ttccgctgga agatatggaa ttttggcaat
     1921 ttcacccgac aggcgtgcat ggagttggtg ttctgattag tgaagcagtg cgcggtgaag
     1981 gtgggtattt gctcaataaa aatggcgaac gctttatgga gcgctatgca ccgaacgcca
     2041 aagatttagc gagtcgtgat gtggtatcgc gtgcattgac tacagaaatc aaagagggca
     2101 gagggtgtgg caaggatggc gattacttgt tactaaaact tgatcatcta ggtccggaga
     2161 tcattaaatc aagattaccc ggtatccgag agattgcgat taaatttgct catgccgatc
     2221 ccatagaaga accgattccc gttgtgccga ctgcgcacta catgatgggt ggaattccca
     2281 ccaattttta tgggcaagtc gttgcacctt ataaaacggg ccctgaagaa gtggtatcag
     2341 gattatatgc agtaggggag tgtgcatgtg tttccgtgca tggtgccaat cgtttaggta
     2401 ccaactcgct actcgatatc gtagtttttg gtcgcgcagc cgggaacaaa attatcgaag
     2461 agcttaaaca taatccccat cataaatcgt taccagagga tatcgctgat atttcattag
     2521 cacgattgaa tcgcctcgat acacaaagta atggtgagaa agtagcggat gtgggtgatg
     2581 cacttcgcag aaccatgcaa acctattgcg gtgtatttcg ctttccggat atgctgaaag
     2641 aaggggttag caaaatcaat gaagtagcgc aacgtgtcgc acgcactgag attaacgata
     2701 aaagcaaaat ttttaatact gctcgaatcg aagcgctgga gttagataat ttaatcgaag
     2761 cagcgattgc cacgatggtt tctgctgagg ctcggcacga aagccgtggt gcgcatacgc
     2821 gtgacgattt cccagagcgc aatgatgaaa aatggttaaa acatacgctc tatttcaaag
     2881 aaggtcagcg cttggattat aagccagttc gattgaaacc attgacagtt gattcatttc
     2941 cacctaaagc gagagtttat tagaaacatc gagagatgtt tcatttttga ataacatgga
     3001 aacgatagga tagtttccat ggtttattat ttgggttaac attgccctcc attatgacta
     3061 agtatgtctt tgtaactggc ggagttgttt cttcattagg taaagggatt gcggctgcat
     3121 cattggctgc attgcttgaa acacgtggta tcaaagtgac catgttgaag cttgaccctt
     3181 atatcaatgt cgatccgggt accatgaatc cattccaaca tggagaggtg tttgtcaccg
     3241 atgacggtgc ggaaaccgat ttggatctag gtcattatga gcgcttcatc agtaccaaaa
     3301 tggccaagcg caataatttt acgacgggtc aaatctacga aagcgttatt cgcaaagaga
     3361 gaagaggcga ttatctcggg gggacagttc aagttatccc ccatattact gatgaaatca
     3421 aattgtttat tcgcaatggc gtgaatgatg ctcaggtggc tattgttgag attgggggta
     3481 cagtagggga tattgaatca ttgccattct tggaagcgat ccggcagatg gcagtacaac
     3541 atccccgtga cgatacttgt ttcatccatt tgacgctttt accttatatt ggttctgcgg
     3601 gtgaattgaa aacaaaacca acccagcatt cggttaagga gttgcgtgaa atcgggatcc
     3661 agccggatgt gttgctttgt cgttccgacc gcatgctgcc ccaagaagag cgtcgcaaaa
     3721 ttgcactatt taccaatgtg aaagaagagg ccgtgatctc tgcgatcgat gtcgattgta
     3781 tttataagat cccagcgtta cttcatgagc aaatgctcga tgagattatc tgtcacaagc
     3841 tcaatatcct cgcaaaaccg gctaatcttt ctgtttggaa aaatctggtt tatgcgctag
     3901 aacatccaac taaaacggtc aatatcgcat tggttggcaa atatgtagat ttgaccgagt
     3961 cgtataaatc tttgtcagaa gcattgattc atgccggcat ccatacgcat tgtaaaatca
     4021 atatctgcta cttggattct gagctcattg aaaaagaagg tacggactgt ttaagcgata
     4081 aggatgcaat tttagttcca ggtggatttg gaaaacgagg ggttgaagga aaaattttag
     4141 ccattcatta tgcacgtacc cataagatac cttatcttgg tatttgtttg ggaatgcaac
     4201 tcgcagtcat cgaatatgca cgcaatgagc tgcatttaga gaatgctcat agtacagaat
     4261 ttaatcccaa taccccttat ccggttcttg gtctcattac agagtggcgg actcgcgaag
     4321 gtcagataga gaagcggtcg gaagctactg atctcggtgg gacgatgcgc ttgggagggc
     4381 aattgtgttt tctcaacgaa gattcattgg ccaaaaagat ctatggatct gataaaatta
     4441 ccgagcgtca ccgccatcgg tatgaagtga atgcgagttt tattcccagc ttggaaaaag
     4501 cgggtctaaa aataagtggc gtatctgctg aagaaaagtt atgcgaaatg gttgaattat
     4561 cccaagacga gcacccttgg tttgttgctt gtcagtttca tccagaattt acatctactc
     4621 cccaaaaagc acacccctta ttcaaagcat atatcaatgc cgcgattagc tacgcaaatc
     4681 agcgtacaga taacagttta gaacagaaag aaattgctta gaaacaaagc aagagagaag
     4741 cgcattagta cattaagtcg attattacaa ggaaaaaagt agcatgagtg cgatagtaga
     4801 tgttattgcg cgagaaattt tagattctcg tggcaatcca accatagaag cagatgtttt
     4861 gttagagtca ggtgcgctgg ggcgcgcatc cgttccgtcg ggggcttcgg ttggaaccaa
     4921 agaagcgatt gaattgcgtg atgaagatga ggaacgctac tttggtaaag gcgtgacaaa
     4981 agcagtagat aatatcaata ccgagatttc cgaaaccatc atggggttag atgcgtcaga
     5041 gcaagcattc attgatcagg cactgattga tttagatggg agtgaaaata agtcaagaat
     5101 gggcgcaaat gccattcttg cagtttcgct ggcagtggcg aaagccgctg cagaagaatc
     5161 cagtttgcct ttatatcgat atttaggggg agtaagagcg atatcgatgc cggttccgat
     5221 gatgaatctc atcaatggtg gtgctcacgc caataataat ttgaatatgc aggagttcat
     5281 ggtgattccg catggttttg acagctttcg tgaagcactg cggtgtgggg ctgagatttt
     5341 tcatacattg aaaaaactga tcagtaaaaa aaatatgcca acaacggtgg gtgatgaagg
     5401 ggggtttgca ccgaatctgt ctggtaatga agaaggatta cagttgattg tgcaagccat
     5461 cgatcaagct ggttatcaac ctggtacaca agttgcgatt gggattgatt gtgcaagttc
     5521 tgaattctac caagatggaa aataccatct taaatccgat ggtttaagcc tgacttctgc
     5581 ccaatttgtc gattatttgg ctacctgggt cgataaatat ccaattatca gcattgaaga
     5641 tggtatgagt gagcatgatt gggatggctg gaaattattg accgaaaggc ttggaaaaac
     5701 tgtccaattg gtgggtgatg atgtttttgt gactaatagc cggatactaa aagagggtat
     5761 tcaacggggt attgcaaatt cgattctgat taaagtgaat cagattggta cgttgacgga
     5821 aactttgact gcagtagaaa tcgccaaatg tgcaggctac acggctgtga tttcgcatcg
     5881 ctcgggtgaa accgaggata cgaccattgc agatatcgca gtcgcaacga atgcacttca
     5941 aatcaaaacg ggttcattgt cacgttccga ccgattagca aaatataacc agttgcttcg
     6001 tatcgaagaa gacttgggtg ataacgcaat ttatgcagga aggagtgcat tctatcaatt
     6061 aacctaatga agactgttgc gctcatcctg gttactttaa ttgcattagt tcaatattcc
     6121 ctgtggtatg gtaaaggtgg gtggttggaa gtctttgagt tggagaaaca actccaagat
     6181 caacaccaat taaatcagaa atcacagaac cagaatacca tcctggaggc agagatcatt
     6241 gatctcaagc agggttttga cgctattgaa gagcttgcga gaaacgagct aggaatgata
     6301 cgtaaagatg aagtcttttt tcaggtacag cagccaacag aagtaccctc tgtaacgaaa
     6361 tctaagaaat agtaagtcta ggttgttgga tttgtttgat gtcttttctg ctggcttgca
     6421 caaagtagcc ggcattttac ttagattgca tgcgtaacca gtgcgctaat ccgagttcac
     6481 cttgccgcat tacttgatcg tggctccatt tgaaaatcgg tctcgcgatc ggtgcaagca
     6541 gattcatcca tagacggttt gtgcgcactg tccaggcata gcggacgatc gttatatcgt
     6601 tcgcgcgtat gaaatcccag cgcccggtac cgaccacttc accttcagct accccttcta
     6661 gcaaaacacc gggtacatag cgcgttactc gtatcgtaaa ggtaaaatga taaggaattt
     6721 tacctttcca agtgaaacga tgcacactac caacgccatc gctatcacct gttaccagtt
     6781 tttccacttg ttcagcccct aaccaccact gaggccaatc caggcattga atgattgcat
     6841 cacataccat ttcgtgcggg gcatcgaagc gccattcagt agtaaaagaa aatgcagccg
     6901 tcactaaaat taatgatcaa aaaaaccttt tgggaaggca gggttaactt gatattctga
     6961 catttcgatt cgttcgattt gactcccgtc tgctttgtaa ctgatgactt taagcggtaa
     7021 gctggtttgt acgtcaaacc acagatcgaa acgcgatacg ccttcaatcg ctacaccagg
     7081 attgccttcc acgatcacat acaacgcagc ttgcttctcc actgtttcgg taccaacgac
     7141 agtcgttttg ccttggcgtt gcagtaatat cacatcctca tacaatgcac caatatcaga
     7201 tcggtcaaca cgctgtcctg atgaactttg tatccatttg ttttcaggac ttaggctaaa
     7261 agaaggtacg ctgccataac caaatagcca caatttgact aattctgtca ctgggttata
     7321 aatcagcact gcaccattga atggttcgac cacttccata cgcactgcac cacttttatt
     7381 gtagtagtag cgcacaatgt ctggactatc ggacttgctt cccacatacg atttgatgat
     7441 cgtttggtag ctatctactt tctgataata atcaaccgct ttttcaatcg ggttgggaag
     7501 ctctggcgcc attagcgcca ttgttaacat taaaccggct agcatgagtc tagattattg
     7561 agtttttcaa acaattacac agggcatacg ataacaggta tcgatgataa ttgcatttgt
     7621 tgagttagat ttttgctaaa tttgtaatac cctgttcacg gtgagtggtg tagtggaaaa
     7681 tgcacagtga gactgaccac aaaattcacg ctatcctttc cttctggcaa aaagaataaa
     7741 actttcttca gcattttctg cattcatcgg ggatgatagc ttgcgggtag caatattgag
     7801 tgtttcttag attacttgat aactaataca agcgctatgt gttttcagtg ctttttgaat
     7861 tcaattgctc tgaaatagtg catttatgct tattagtttc atcgaaccag tagatgaaca
     7921 tattgatatc aacaaaatta ccactcattc atttcatctc gggcaaattt gcgaccacca
     7981 gttcgcattt gttcgcgtaa tttcttaatt aaagccattg cagacctagt ttcttgcttc
     8041 tgctgaacat gagtgagtca ctgtctttaa gctaaactta ttcctggaac tgtttagaat
     8101 aacaatcgaa ttcagtttca aacacaatat tttcatacac ttcgttcatc tcgcaattat
     8161 tgccagccgg taacttcata gcccttgtct tgcaagcagc gataaacaaa attgacatag
     8221 gcttggttgg gttgtgtcgg tctcgccaaa ccaaatagac cacgaatcaa cccaagcgca
     8281 gcgccactgg cagcaccgat taaggagcca gtacccgctg caccggaaat cgctccccca
     8341 actgcaccgc ttgcagcacc catcccggca cccattgcag tactggtcgc gacattaccg
     8401 acctggctgg cattaccttc ttgcgcaccg gcttcttctg ctaactgacg gcaggcaaca
     8461 atatcctgct cagcagcgtg ttgtccaact gttttgtaat gtgtattggg atacaaaatc
     8521 ggccgtgtgg tggaacaggc tgtgagtgcc aaaatgaata gacctaaaat gaagatatgg
     8581 aatttcatgc gaattgccct caaaaaaaca ttgctaaaat acaagttgaa tttatcacac
     8641 ttctagcgcc acagttgagt tcagatagct acatggggat gaagctacct caataaacga
     8701 ttacaaatgt gatttttctc acagatttac caatttgatc aagttaacct gtgcttgctt
     8761 ggtaattcaa ctttccattg acacatactt tgcaaatgaa acgcttcaat accacagtcg
     8821 aaagtgttta accaagcctt cccatagcat gcgcctattc cagtgagagt tgagtgcatg
     8881 atcaaataat tattattagg aggaccacaa aatgaccgta actttgacaa atctcgatga
     8941 caactatatt gatggacccg gcggtaatga aatttttgcc cagctgggca atgatactgt
     9001 caatggggct gatggcagtg acacgatttg gggggaaggc ggtaatgatt ccattctggg
     9061 tggaaaccaa aacgacacac tgtatggcgg gatcggaaac gataccttaa atggcggttc
     9121 tggcgtcgac cgactggaag gtggcgcaga taacgatacc tacattatcg actccaatgg
     9181 aattttttta cctctcgata ctatcgtgga tagtggtgga atcgatacag tattaagttc
     9241 agtaacctat acactgggtg ctgccatcga gaatctaacc ttaaccggtg cagctgcaac
     9301 aagtggtact ggtaatgttt ctgctaatgt tataactggt aacagcgcca acaacacttt
     9361 aaacggatta aacggtaatg acactctgaa tgctggcttg ggtaatgatg tgctcaacgg
     9421 cggtgatcaa aatgacacgt tgaatggtga tggtggtgtt ttagcattat ccggtggtaa
     9481 cgatacgctc aatggcggca atggcaatga tactctctca ggcggcctgg gcaatgatgt
     9541 gctcaatggt gatgttggcg atgatcggct agttggcggt tctggaaatt tctttcaacc
     9601 tattgatgtc gataccctaa gcggtggtat tggcaacgat acctacgtga tctcggaaaa
     9661 taccgatgtg attaccgaat tatcaggtga aggaaccgat accgttgaaa gttcgattac
     9721 gtttaattta ggcacgagcg gtgcaaatgt tgaaaattta acattaaccg gaacagcagt
     9781 gatcaacgga acaggtaata ctttaagtaa cgttatcaac ggtaattcaa aggataatac
     9841 acttgatggc ggtgcgggca acgataccct taaaggaaat gatggcaacg atactttgat
     9901 tggtgggcct ggtctcaccg ttgtcattgg accctttggt cccacgttca ttagcgataa
     9961 tgatacgctt gaaggtggca aaggcaatga cacttatatc atggattata cgggcgacac
    10021 catcactgaa aatctcaacg aagggattga tcttgtaaaa agctcactga gttcatatac
    10081 gcttccaacc aatgttgaga atctcaccct aactggggtt ggtaatatca atggtcttgg
    10141 caatagcctt gataacgtaa ttactggcaa tgcagggatt aatgcgctca gtggcgatat
    10201 tggtaacgac accttgaatg gtggcggtgg aaatgatgtg ctcgatggcg ggcaaggtaa
    10261 cgatacctat atcgctgatg ggggtgacac gatatctgaa gcagctggtg ccggtactga
    10321 tagagttgaa agttctatca cttttagcct tgcagctatc gcaaacgttg agaatcttac
    10381 gcttaccggt gcagctaata tcaatggtac gggtaattcc cttaataaca cactcgttgg
    10441 taatagtggc accaatacct tgaccggttc agggggtaat gatgttctca tcgggggtgc
    10501 cggtgctgat attttgaatg ggggtaatga tgatgatacc ttctggtacg atgcaactga
    10561 tgttagcgta gcgggtaacc gaatttctgg tggatcgggt actgatactt tgacattcat
    10621 gagcagcgct gtacagaatc tcaatctcac cacactcgca gatgatcgca ttaccggtat
    10681 tgagcaaatc aatatcaatg gtttcaatct attcggacca tcacccaaca acacactcac
    10741 gttgaatgtt accgatgtat tcgcgatctc ggataccgat acgctgcgag ttgatggtgg
    10801 agtgggtgat gttgtcaatt caatcggaca agggtgggcc aataccggtg ttgtggttat
    10861 tggaacgcag aactatgatc aatatacgtt tgggactgct acgctgttag ttgataccga
    10921 catcggtggc accatcagtt agttagcaag ctgaatttag cttgaattaa attcttctaa
    10981 ccggggggta acgatgcagt agatcgttgc ccccaacacc cactctcagg cgcgagtgcc
    11041 taaaatccta aaatactttc ccttttgcgt ccaaactaaa accagctaag ttaatgcttg
    11101 gtttacgttt taatccccca aaccggttaa gaaacgctat gatcatgcaa aattttctga
    11161 ttaaaaatat aacgaagaaa aacagcatga ttgaaaaaga gttgaaatta ttcggaatgg
    11221 cagcaagcca aggccgatgg ttatttgtgc taattggact gtcgatcaat atttgcttgg
    11281 gatcgattta cgcttacagt gtatttagaa aaccattaga aaccctgtgg gacattccat
    11341 cgagccaaag cggttaccca ttcatgatct ttcttgcagt cttcgcaatc accatgccgc
    11401 ttgccgggag cttgatggcg cattggggtc ccagaaaaac agcaacgctc ggcggcttgt
    11461 tagtgggtat tggctggatg gctgctagct ttgctccgga tattactaca ttgacacttt
    11521 tatacggcgt catcgggggt gctggtgtgg ggatcgtgta tggttgccca atcgccgttg
    11581 tcgccaagtg gttccctcaa aaaagcggct tggcgattgg tttgacggtg atggggtttg
    11641 gtctctcggc actgattacc gctcctttga tgaaagcgat gatcattcac ccggaaattg
    11701 gcaccctcaa tacttttttc tatttaggcg tcagcttttt gtttatcgtc tcattattag
    11761 cacgatgcct tcgttttccg cctgatgaat ggtcatcagt taatgtaatt acccattcct
    11821 ctactacgac agccaatccc acgattgagt taagccggca tgacatgata agaaccaggc
    11881 atttttatgc attatgggcg acctatacaa tcggttgctt agccggactc atggcaattg
    11941 gaatagcttc cccagtgggt atcgaggtcg caaaactaga accgacaacg gcagcattag
    12001 cgatttcatt atttgcgata ttcaatgggt tagggcgccc tttgtttggg actatcgctg
    12061 ataaactcgg gccgaaacaa acggcaatgc tctcatttgc catgatcggt ggtgcgtcac
    12121 tgctgttatc aggcttggaa gaaggcgata ctaccttgta tttatttgct ttttgtgtgt
    12181 tatggctgtg cttagggggc tggctagcat tggcgcccac ttccactgga attttctttg
    12241 gaaaaaaaca ttatgcccag aattatggcg tggtttatac cgcttatggt gtgggtgcca
    12301 ttttgggaac actgatctct ggcaatatca aagatatcac gggtagttat ttaggtgcat
    12361 ttccgattgt tgcaggatta gcggtgcttg gcttgttgat tgctgcagta gggttaaatt
    12421 cggttaaaaa caaatagagt agttgtgaag atgggtaatc aataaaaggt ccaccatgtt
    12481 taatcaacat catttctggg cattaatctg cggtccttta cttgctggag ttgttgctgc
    12541 gagtatgcat ttctcaggtt ggcatgtaga agcctgttgg acggctgcta ttgttttatt
    12601 gtgtgttatc tggtggattt ttgaaccgat acctatcccc gcaacttcgc tgattcccat
    12661 cacatttttt ccgctaacag gggttctggc caaagaagaa gttgccaagg cctttggcaa
    12721 cgacttgatt ttgctgatgt tgggtggttt tatgatttcg gtggcaatgg cgcaatcagg
    12781 aacgcatcgg cgcctggcat tagggttagt gaatttcatc ggtggcgaga gtagtcggcg
    12841 catcgttttt ggttttatgt gtgcctgtgc ggtattaagt atgtggattt ccaatacggc
    12901 gactgcattg atgatgctcc cacttgcgct cgcgatcatc gatcagagtc aggataaaaa
    12961 aattgccttc ccgctgttac tttccatcgc ctatggttgc agtattgggg gcttaggtac
    13021 gccaatcggc acaccgccca atctgatttt tatgaaagag tatcagagct ttaccggatc
    13081 aacggtcagc ttttctcagt ggatgatatg gggacttccc gtggtgtttc taatggtgcc
    13141 acttgcggga ttatggttga cgcgtcattt aagttacgtc ggtaagctag aaatgccaca
    13201 agccggtgct tggcggcctg aagagcggcg cgttctgatt gtctttggta ttaccgtgtt
    13261 ggcctggatt acacggcttg aaccgtttgg gggctggagc acctggttgg agttaaaaaa
    13321 tgcaaccgat gcaacggttg cgttgatcgc tgtaattgtg atgtttatca ttcccaatgg
    13381 ccggggcgaa aaattgctta actgggaagc ggccgccaag attccttggg ggattttatt
    13441 gcttttcgca gcgggtatcg ccattgcaca agcgtttatt gaatcgggtt tatccaaatt
    13501 cattgcagag caattagcga tactttcaaa cttgcaccct ttggttatta tcgtatcgat
    13561 tgcgctagct gtaacatttc tgaccgaggc caccagtaat gcggccacta cgatattgct
    13621 catgcccatt cttgctcctg ctgcattggc tgctgggctt gatcctgctt tattgatggt
    13681 gcctgctgcc atgagtgcaa gctgtgcatt tatgcttcct gttgctacgc caccgaatgc
    13741 aatcgttttt ggaactggaa aaattccgat cgcaggaatg gtgcgtgaag ggttggcgct
    13801 taattttatc ggtgtgacgg ttattactac agtttgttac tttctagtac catagcgaaa
    13861 cgatttagaa cggatactta aattgaggga aaaatatgat tacgctgtat ggaattgaat
    13921 ggtcacgcgc aaaatatgta t
//
LOCUS       DAOQKQ010000057        13937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_2056_length_13937_cov_6.019810, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000057 DAOQKQ010000000
VERSION     DAOQKQ010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 13937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13937
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2056_length_13937_cov_6.019810"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(293..454)
                     /locus_tag="PKZ49_05280"
     CDS             complement(293..454)
                     /locus_tag="PKZ49_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36010.1"
                     /translation="MKNLTIQMNGGNEHVLFENRLPWNTEYSREYLPNITRLEKGERN
                     GFYARHMII"
     gene            544..1512
                     /locus_tag="PKZ49_05285"
     CDS             544..1512
                     /locus_tag="PKZ49_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36011.1"
                     /translation="MGIYYGDNSNNHLWEPYPKTESDKMFGFGGNDFIEGGYGNDSLY
                     GGSGNDQVNGDEGNDILYGESGIDTLLGGEGNDWLYGQSEIDRLEGGNGNDVLDGGTG
                     ADWMFGGLGDDYYYVDNVGDYIWETDGWDAVASEVYSYTLPTGVERLNLGENGVIGYG
                     NALNNLIFDYGNVGVSYLYGGYGNDTLWGYTGDDFLAGEWGNDTIYGGDNIDTIAGGA
                     NSDTLWGEGGADYFTYYDRSESINNSYGWDIIKDFNRWEGDKIDLSWLDANLNKSGSQ
                     AFTVDHFNYYGWGNNGYLEVYTIGGSTMLIELAGAPQLNIATDIIM"
     gene            complement(1587..2651)
                     /locus_tag="PKZ49_05290"
     CDS             complement(1587..2651)
                     /locus_tag="PKZ49_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid 1,2-hydratase"
                     /protein_id="HND36012.1"
                     /translation="MRLLLILLALIGLGIGVAAPPILLPVTPRELVFPEDFGAHENFR
                     IEWWYATGWLETTDQKTVGFQVTFFRPTTEYDQDNPSQFAPKRLIIGHAAISDPAVGR
                     LLHVEKTVREGFGLAYAKAGNTDVKLDDWYFIRDTDGSYRTVVQGNDFSLELRLEPSQ
                     PLMLQDQAGFSRKGPGSEQASYYYSEPHLKVTGKMVRAGNTTEVKGTAWLDHEWSSEY
                     LDPNAEGWDWTGINLDDGSALMAFQIRGKQGEKIWAYAGLRDASGRMTIFSPEEVSFH
                     PVRTWKSPHTGATYPVAMRIMTEETEWVLTPLMDDQELDSTQSTGAVYWEGAVTVIRN
                     GQPAGKGYLELTGYKKALSM"
     gene            complement(2651..5203)
                     /locus_tag="PKZ49_05295"
     CDS             complement(2651..5203)
                     /locus_tag="PKZ49_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HND36013.1"
                     /translation="MKRNLLSQWLLQGQWHSHRLQMSMVMIAIALGIALGFSIQLINS
                     AAIHEFSSAIKILSGQSDLQIRAVQPTFDEMLYPKLAQREGVELASPVLEIQAAIPGQ
                     LDDTRNGMLKILGIDVFRAMAMTPDIVGVPAEDRNFDTLAIDAIFLSPAAMEWLKVAQ
                     GDSLSLRLGTKTVTLRVAGGLVRANPGQRLAVMDIGAAQWHFDHVGLLSRVDLKLKQS
                     VNLEQFKASLEKELSSQYLVAETIDQEKRINNMSRAYRINLNVLALVALFTGIFLVFA
                     TQFLATLQRRPQLALLRVLGMTKKQILLQILKESSLLGLLGALLGIVLGYGFAISALY
                     FLGGDLGAGFFPDIQPQIQFDPWLTLLFLMLGIGVTLMGSITPAWEAANAHPAQALKS
                     GSEALSMTRLNKPWAATACIIIALIFTQLPPVSELPLFGYFAVALLLIGGIAWMPTLA
                     KLFFSLLLAFTNRYPRNVLTQLVLARLSNTSHQAAIALGGVLASFSLMVAMAIMVASF
                     RVSIEHWLDHILPADIYVRTELRQDIIGFTRSEQNALANASGIERIDFIYSQQISLDS
                     SRPSVTLFARPIDKNDPGSVLPLTDDVLPTETLANSDAIPVWVSEAMVDLYDFQVGKQ
                     IELPLGKTLHNYIVAGVWRDYGRQFGAIQMRLSDYQTVSGEFHINEAAVWLTDGVSTQ
                     QMMNTLRALPFGEALVFWQPNEIRSLSLKIFDRSFAVTYLLEIIAVLVGLLGVAASFS
                     AQVLTRIKEFGILRHMGLTRGQIIRMLTLEGGLLTGFGIAIGFILGGSISLILIFIVN
                     PQSFHWTMQLYMPWNWLALMIIALLFSAALTAYIVGKSAVARQAVQSVKEDW"
     gene            complement(5209..5877)
                     /locus_tag="PKZ49_05300"
     CDS             complement(5209..5877)
                     /locus_tag="PKZ49_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HND36014.1"
                     /translation="MEHPFALELRNIDKAFASKRQIFSRLSFSLRQGEYVAITGESGI
                     GKSTLLNLIAGLDFPDGGEILINGSLLPKDDNAATLLRRQHFGFIFQAFHILPHLNLL
                     QNVALPLLLNQFPIDRAQAMLTAVGLEGRAQDFPHQLSGGELQRVAIARALVHQPKLI
                     LADEPTGNLDPDTAREILYLLQSEIKASGAAAIIVTHSQFAAQTADRVLQLTASNLIS
                     VDHP"
     gene            6144..6731
                     /locus_tag="PKZ49_05305"
     CDS             6144..6731
                     /locus_tag="PKZ49_05305"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HND36015.1"
                     /translation="MQSLATRFSFITTQKHTTLKKGAQYLLAILMVVNLMLFSRPAHS
                     SESVEYQVKAAFISNFIAFTAWPGDSNESIQLCLYGEDMFGTSLDQIAQKPNSKGKIK
                     VRRINDESTLTECQVVFIAKSNISNLSNILNTLREKPILSLADSPNAASQGVNINMNV
                     NSDGKVTFEINLATARLSKLDFSSKLLQLATHVYR"
     gene            6738..8768
                     /locus_tag="PKZ49_05310"
     CDS             6738..8768
                     /locus_tag="PKZ49_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HND36016.1"
                     /translation="MKIINRTLAFALMLLILLSTNAISGKPGDNQFLELSIEELMNVK
                     VTTVSRRAQKLSEVASAIFVITQDDIRRSGATSIPDALRMAPGVQVDRVSTGQWAVSI
                     RGFNGIYANKLQVLMDGRSVYSPIFSGVLWEQQDTLIEDIERIEVIRGPAATVWGANA
                     VNGVINIITKKAAETQGTLLSAGGGSFEHGFFGARYGGKINEETPFRVYAKGFTRDHM
                     ISPEGANRHDQWHSARGGFRLDHARGLDQFTLQGDIYFNSFGNSVDKNTSQLLALQTQ
                     GLRGNQEGGNIRFRWDRVYSERSSIMLQTYYDRNTHQLLPIGKSDAESFDIDFQHRLP
                     LFDKHDVIWGANYRLYNNKIFDTLLTSASPRERTHHQFSGFIRDDVALIPDRLLFSLG
                     TRLDHNDFSGLEVQPNARLMWTPNAQNSIWASISRAVRIPSRGENDITLNAGQLQSLP
                     GIPTLPIPISILMQGANNFGSEKLLAYELGYRHQFSQQASIDIAGFINDYSHLRDLSF
                     GALSFSTGLPRQLILPLLLNNRASALTYGFEVSADWKPTNRWRLQGNYSFLDISTSSN
                     ELLKTFDPTTGGAAKVNPQHQFSVRSNYDISDRLQFNLWLRYTSAIGFYNIPGFVTMD
                     TKLAFKPVKDVELFLVGQNLFSEYHREFVSDNIPSIPALIPRGIYVGARWQF"
     gene            8953..9339
                     /locus_tag="PKZ49_05315"
     CDS             8953..9339
                     /locus_tag="PKZ49_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36017.1"
                     /translation="MKTRHLLVLLAFVAQSFAVNAESTIAPSNHESLAQHHLSLVDEA
                     KVKLQEHKNNLEDYELHSYYYGRGGQDFRSHETANVRLYEEIIAENLKQAEFHQQMAE
                     AEKNQINKANINNFAAGEVSSAESTH"
     gene            9412..9753
                     /locus_tag="PKZ49_05320"
     CDS             9412..9753
                     /locus_tag="PKZ49_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36018.1"
                     /translation="MKKFFFSVLILTFLVTNLSVAAESENTATAKNKSPLSHEEIAQR
                     YDNKAKRMIDKIAEEKQMLDEYELHSYYYGREGQDFASHHEALLHKFEKAAKRYEELA
                     AAHREMARKTN"
     gene            complement(9849..10781)
                     /locus_tag="PKZ49_05325"
     CDS             complement(9849..10781)
                     /locus_tag="PKZ49_05325"
                     /inference="COORDINATES: protein motif:HMM:NF013246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HND36019.1"
                     /translation="MIVKDKVSWVRLLFHFRGSSFMETWPRILTVTVTASVVTYIELY
                     YSIQAYTLTTTPFLLVGVALGIFLGFRNSASYDKFWEGRKLWGALVNTARSLTRQICH
                     MTDSRQSCETLQPFQAMFVKRIIAFAHALRCHLRNQEPFEEIKKFLPADDYAAVAVAK
                     HRPLIILQQLSRDLAIARDRRWLSEYNHPFLEAQLNEFSNILGGCERIKNTPIPFSYS
                     VLTHRIVAAYCLSLPFGLVETTGILTPVVVLMISYAFFELDAIGDEIESPFGLQPNDL
                     ALTAISRNIEINLLELINDADQPDIVKPVDDILI"
     gene            11075..13495
                     /locus_tag="PKZ49_05330"
     CDS             11075..13495
                     /locus_tag="PKZ49_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HND36020.1"
                     /translation="MNKKQLSERDICTKFITPALVRAGWDISSQIREEFPLTKGRIIV
                     RGKLHTRAERKRADYVLFYKSNIPIAIIEAKDNNHTVGDGMQQALGYAEMLQVPFVFS
                     SNGDGFLFHNKIAQDGIVERELALHEFPDAEMLWQWWVKHLGLNNQQNSLVTQDYYSD
                     GSNKNPRYYQLLAINKTIEAIARGQNRILLVMAAGTGKTYTAFQIIWRLWKSKTKKRI
                     LFLADRNILIDQTMTNDFKPFGSSMAKIQNRQVNKSYEIYLSLYQAVTGTEEEKNIYK
                     QFSPNFFDLIVIDECHRGSAATDSAWREILEYFSSATQIGLTATPKETKDVSNIDYFG
                     DPIYTYSLKQGIDDGFLAPYKVVRIDLDKDLTGWRPDKGMLDKHGFEIEDRIYNQQDF
                     DKTLVLEKRTQMVAKKISEFLKQTNRFDKTIVFCENIDHAERMRQALINENADLAGQN
                     NKYVMRITGDNEEGKAELDNFIFPESKYPVIATTSKLMTTGVDAQTCKLIVLDQRIQS
                     MTEFKQIIGRGTRINEDYGKYFFTILDFKKATELFADPDFDGDPVQIYEPKGTESPVP
                     PDISVEGASGESMIYSSSDTNPIWTGAAQTDPDNETYRVRRFTVANVEVKVVAERVQY
                     FDATGRLITESLKDYTRKAVAREYGSLDDFLRRWNSADRKQVIINELSEQGVFFDALA
                     DEIGQQSGKLFDPFDLLCHVAWDMPPLTRKERVEQVKKRNYFTRYGEQAQRVLQALLD
                     KYADEGMAQIEETQILTIAPFTGFGTPMEIIRAFGGLDQYQQAVHDLEDALYLPDRHS
                     RESGNPGG"
     gene            13497..13784
                     /locus_tag="PKZ49_05335"
     CDS             13497..13784
                     /locus_tag="PKZ49_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HND36021.1"
                     /translation="MKQPCVYILASQKNGTLYTGVTSDLIKRVWEHKHDQAEGFTSKY
                     AVHHLVYFEQHEDMNAAITREKQIKKWNRAWKLRLIEALNPNWNDLYETLI"
BASE COUNT         4171 a         3119 c         2939 g         3708 t
ORIGIN      
        1 cctattgatc ttgctatata agatgaaaaa cactgagaat agttcatagg cattatcttt
       61 aataacacca ttacaaaaca tttttgctga attcctcaca ccggttgcct catattttgg
      121 tgatataaat cgcaccagcc acccatctgg atcaagtgaa gacagtagtt ttttaataaa
      181 aatcgaatct gataatcctc tagccagctt cctcgcagtc agttgttaaa ctgactgctc
      241 caataacgat gaggagactg acatgcaaca ttactgcgga attgatttac attcaaataa
      301 tcatgtgtct tgcataaaac ccatttcttt cacctttctc aagcctcgtt atattcggta
      361 aatactccct tgaatactca gtattccaag gtaaacgatt ctcaaacaaa acatgttcgt
      421 tcccgccgtt catttgaatt gtgagatttt tcacatattt tcttaaggct tttacttaat
      481 ctttaaacac tatcgattga tagcaaacaa gattctgatt ctaatcgtta aaaggagaaa
      541 aaaatgggta tatattatgg cgataatagt aataatcatc tttgggaacc ttatcctaag
      601 actgagagcg acaaaatgtt cggttttggt ggtaatgatt ttattgaagg tggttacggt
      661 aatgactccc tatatggtgg ttcaggaaat gatcaagtaa acggtgatga gggcaatgac
      721 atcttatatg gcgagtcggg tatagataca ctcctgggtg gagaaggaaa tgattggcta
      781 tatggacaaa gtgaaattga tcgacttgaa gggggtaatg gaaatgacgt attagacggg
      841 ggcaccggag ccgattggat gttcggcggt ttgggtgatg attattatta tgtggataat
      901 gtaggtgact atatctggga aaccgatggc tgggatgcgg tcgctagcga agtttattcc
      961 tacactttac cgaccggtgt cgagagattg aatctaggag aaaatggggt tatcggctat
     1021 ggaaatgctt tgaataacct tatttttgat tatggtaatg ttggggtcag ttacctgtat
     1081 ggcgggtacg ggaatgatac gctttggggt tataccggag atgattttct cgctggagaa
     1141 tggggtaatg ataccattta cgggggggat aatatcgata ccatcgcggg tggcgctaat
     1201 tctgataccc tttgggggga aggaggtgct gattacttta cctactacga ccgaagtgag
     1261 agtatcaata attcctatgg ctgggatatc atcaaagatt tcaatcgttg ggaaggtgat
     1321 aagatagatc tatcctggtt agatgcaaac ctgaataaat caggtagcca agcattcacc
     1381 gtagatcatt ttaattatta tggttggggt aacaacggct atcttgaagt atataccatc
     1441 ggcgggtcaa ccatgttaat cgagctagca ggagcgccac aattaaatat agctaccgat
     1501 attattatgt agtccggaaa aagatccctg ttttgatcat gataaatcag gggtcttgtt
     1561 taaatagtga tcagatcgac gagcgatcac atcgataatg cttttttata ccctgttaat
     1621 tctagataac cttttccagc aggttgtccg ttgcgaataa ctgttaccgc accttcccag
     1681 taaactgccc cagtggattg cgtggaatcc agttcctggt catccatcag cggtgtcagt
     1741 acccattcgg tttcttccgt catgattcgc atcgcgaccg gataggttgc accggtatgc
     1801 ggagatttcc aagttcttac tggatggaag ctaacttctt caggtgagaa aatcgtcatg
     1861 cggcctgagg catcacgtag tcctgcataa gcccaaattt tctcgccctg cttgccacga
     1921 atttggaagg ccatcaacgc agaaccatca tcgagattaa tcccggtcca atcccatcct
     1981 tctgcatttg ggtcaagata ttcggatgac cattcatggt cgagccaggc agttcctttg
     2041 acttcggtcg tgttacccgc gcgtaccatt ttaccggtta ctttcaaatg tggttcacta
     2101 taataatagc tcgcttgctc agatccgggg cctttccgag aaaaccctgc ttgatcctgc
     2161 aacatgaggg gttgactggg ttctaaccgc aactccaggg aaaaatcatt tccctgcaca
     2221 accgtacgat aacttccgtc tgtatcgcgg ataaaatacc aatcgtctaa tttgacatct
     2281 gtattacccg ctttagcata ggccaggcca aaaccttcgc gcacagtttt ttcaacgtgc
     2341 aataacctac ccaccgctgg atctgatatc gcagcatgac caatgatcaa ccgtttcggt
     2401 gcaaattgac tgggattatc ttgatcatac tcggtggttg ggcgaaagaa agtgacttga
     2461 aatccgactg ttttttgatc ggtggtttct aaccaccctg ttgcatacca ccattcaatg
     2521 cggaaatttt catgtgcacc aaaatcttct ggaaaaacca attcccgagg ggtaaccggt
     2581 aataatatcg gtggagcagc cacaccaatc cccaatccaa ttaatgcgag caagataagc
     2641 agtaaacgca tcaccaatct tccttgactg attgcactgc ctgccttgcc actgcagatt
     2701 ttccaacgat gtaggcggtc agcgccgctg aaaacaataa agcaatgatc atgagagcaa
     2761 gccagttcca cggcatatac agttgcatcg tccagtgaaa tgattgtggg ttgacaatga
     2821 aaatgagaat caagctgata ctacccccca aaataaaacc aattgcgata ccaaagccgg
     2881 tcaagagacc accttccaat gtgagcatgc ggataatttg gccgcgtgtt agacccatgt
     2941 gccgcagtat tccgaattcc ttaattcttg ttaacacttg ggcagaaaaa ctggctgcca
     3001 cgcctaacaa tcccaccagc accgcaatta tctcaagcaa gtaggtaacc gcaaaactcc
     3061 gatcaaagat ttttaagcta agggatcgaa tctcattcgg ttgccaaaat accaatgctt
     3121 ccccaaaggg caatgctctt aatgtgttca tcatttgttg ggtcgaaaca ccgtcagtta
     3181 gccaaaccgc tgcttcgttg atatgaaatt cacccgatac agtctgataa tcgctaagcc
     3241 gcatttgaat cgcaccgaat tgtcgtccgt aatcgcgcca gacaccggcc acgatatagt
     3301 tatgcagtgt cttgcctaaa ggaagttcga tttgttttcc cacctgaaaa tcgtaaagat
     3361 caaccatcgc ttccgatacc caaaccggta tcgcatcgct attggctaac gtttccgtgg
     3421 gtagaacatc atccgttaaa ggtaatacac tcccgggatc atttttatca atagggcgag
     3481 caaataacgt tacactcggc cgtgacgaat ctaagctaat ctgttgcgag taaataaaat
     3541 cgattcgttc gataccactc gcgttggcca atgcattctg ctctgatcgc gtaaatccga
     3601 ttatgtcctg gcgcaactcg gttctaacat agatatctgc aggtaaaata tgatctagcc
     3661 aatgttcaat cgatacacgg aaactggcaa ccatgatcgc catggcaacc atcaaactaa
     3721 agcttgcgag cacgccaccc aatgcaatgg cggcttgatg agatgtatta ctcaatcttg
     3781 ccaatacgag ttgggttaga acattacgtg gataacgatt agtaaatgct aataataatg
     3841 agaaaaatag ctttgctagc gttggcatcc atgcgattcc accgattagc agtaaagcaa
     3901 ctgcaaaata accaaacaag ggtaattctg aaactggcgg aagctgcgta aagatcagtg
     3961 caataataat gcaggccgtt gctgcccacg gtttattcaa ccgggtcatc gaaagcgctt
     4021 cactaccgga tttcagtgct tgagctgggt gagcattagc cgcttcccaa gcgggtgtga
     4081 tacttcccat tagcgttaca ccgatgccta gcattaaaaa taggagtgta agccaaggat
     4141 caaattgaat ttggggttga atatcgggaa agaaacccgc tcccaaatcc ccgcccaaga
     4201 aatacaaggc agatattgca aaaccatagc ccaatacaat gcctaagaga gcgcctaaca
     4261 atcccagcaa actactttct tttaaaattt gtagcaaaat ctgctttttt gtcatgccta
     4321 aaacgcgcaa caaagccagt tgcggccttc tttgaagcgt ggcaagaaat tgtgtggcaa
     4381 ataccaagaa tatccctgta aataatgcca ccaatgccag cacattgaga ttaatgcggt
     4441 aagcacggga catattgttg atgcgcttct cctgatcgat ggtttctgcc acaagatact
     4501 gagaagacaa ctctttttct aatgaagcct tgaattgttc taaattaacc gattgcttta
     4561 atttgagatc aactcgcgat aatagtccga cgtgatcaaa atgccactgt gccgcaccga
     4621 tatccataac ggcaagacgc tgccccggat ttgcgcgcac caacccacct gctactctta
     4681 aagttactgt tttcgtacct aagcgcaaac ttaaggaatc cccttgcgct acctttaacc
     4741 attccatggc agcaggtgat aaaaagattg catcgattgc caaggtatca aagttccgat
     4801 cttctgcagg cacaccgacg atatcaggtg tcatggccat tgctcgaaaa acgtcaatcc
     4861 cgagaatttt cagcatacca tttcgcgtat catccaattg gccaggaatt gcagcctgaa
     4921 tctctagcac tgggctagcc aattcaaccc cttcgcgctg cgcaagctta ggataaagca
     4981 tttcatcgaa ggtaggctga accgcgcgta tctgcagatc agattgtcct gataaaattt
     5041 tgatcgctga ggaaaattca tgaattgcag ctgaattaat gagctgaatc gaaaacccca
     5101 atgcaatgcc taaggcgatc gcaatcatta ccatcgacat ttgcaaccga tgactatgcc
     5161 actgcccttg taataaccac tgcgataaca aattacgttt catggcactc aagggtgatc
     5221 aaccgagatc aaattagacg cagtcagttg caaaacgcga tcagcggttt gggctgcaaa
     5281 ctgagaatgc gtgacaataa tggcagcagc gccactagcc ttgatttcag actgaagcaa
     5341 gtaaagaatt tcgcgcgctg tatctggatc aagatttcca gtgggttcat ccgctagaat
     5401 taatttaggt tggtgaacca aagcacgggc aattgcaacc cgctgcaact cacctcctga
     5461 tagctggtgc ggaaaatcct gcgctcgacc ttctaaacca accgcagtca gcatcgcttg
     5521 agcacggtca atcggaaatt gattcaatag caagggaagc gccacattct gtaataggtt
     5581 caaatgggga aggatatgaa acgcttgaaa aataaaacca aaatgttgtc ttctcagtaa
     5641 cgtcgcggcg ttgtcatcct taggcaataa gcttccatta atcaaaatct cccctccatc
     5701 tgggaaatct aggccagcga tcaaattcaa taacgttgat ttaccgatac ccgattcacc
     5761 ggtaattgca acatattcac cctgtcggag cgagaaagat aagcgagaaa atatctgtct
     5821 tttactcgca aaagccttat caatgtttct taattcaagc gcaaatggat gttccatgta
     5881 aatttaaatg ttaccaaaga aagcgttgta gaaaagcgag gttcgatagg gcacttcata
     5941 tcaaaaataa agcgataaat cgctatttaa tcaaacgatc aatcctgatg actcgtttta
     6001 acataacaag gttcaaatta acgtaataag gcacttactc taacttcaca gagatagtta
     6061 attatcaagg ccctgttttg caattgttaa tctcatttaa acgatgattt attggcaata
     6121 tggctgagta atacgcttat ttaatgcaat cactcgctac tcgatttagt tttataacca
     6181 ctcagaagca cactacgctt aagaaaggtg cgcagtactt gctcgccata ctaatggttg
     6241 taaatctgat gctattttct cgtccagctc attccagcga aagcgttgaa tatcaggtca
     6301 aagcagcatt catctcgaat tttatcgcat ttaccgcgtg gccaggtgat tccaatgaaa
     6361 gcattcaact atgtctttac ggtgaagata tgtttggcac aagcctggat caaatagcac
     6421 aaaaacctaa tagcaaagga aaaatcaaag ttaggcgcat caatgatgaa agcacattaa
     6481 ctgaatgcca agtcgttttc attgccaagt ccaatataag caatctatcg aacatcctga
     6541 ataccctacg agagaagcct atattatcgc tggcagatag ccctaatgcc gcatctcagg
     6601 gagtcaacat caatatgaat gtcaatagtg atggaaaagt cactttcgaa atcaatctag
     6661 caactgcgcg tctttctaaa ctcgatttta gctcaaagtt gctacagcta gcgacccatg
     6721 tttatcggta agaacctatg aaaataataa atcgaaccct cgctttcgcc ctaatgctct
     6781 tgattttatt gagtactaac gccatcagcg gaaaacctgg tgacaatcaa tttcttgagt
     6841 tgagcattga agaattaatg aatgtcaaag tgacgactgt ctcaaggcgt gcacagaaat
     6901 taagtgaggt agcttcagcg atctttgtca tcacgcaaga cgatatccgc cgctcgggtg
     6961 caaccagcat tccagacgca ctcagaatgg cccctggcgt tcaggtcgat cgtgtcagca
     7021 ctggtcaatg ggcggttagt attcgtggct ttaatggaat ttatgcgaac aaattacaag
     7081 ttctgatgga tggtagaagt gtttatagcc ctattttttc tggggtattg tgggaacaac
     7141 aagataccct gatcgaagac atcgagcgca ttgaggtgat tcgcggccct gcagcaacgg
     7201 tttggggagc gaatgcagtc aatggcgtta tcaatatcat taccaagaaa gcggctgaga
     7261 ctcaaggaac gctcctttct gcaggagggg gtagttttga gcacggcttt tttggagccc
     7321 gttatggtgg aaagatcaat gaagaaaccc ctttccgagt ttatgcaaaa gggttcacgc
     7381 gtgaccatat gatttctccc gagggtgcta atcgccatga tcaatggcac tcagcgcgag
     7441 gtggttttcg tttagatcat gcgagaggac tcgatcaatt tacgctacag ggcgatatct
     7501 actttaattc ttttggcaat tcagttgata aaaatacttc tcaattgctt gcgctgcaaa
     7561 ctcaaggatt gaggggaaat caggaaggcg gcaatatccg gtttcgctgg gatcgcgttt
     7621 attcagaacg atcgtctatc atgctgcaaa cctattatga ccgaaacact caccaattgc
     7681 tgccaatagg aaaatccgat gcagaaagct tcgatattga tttccaacat cgacttccgc
     7741 tctttgataa gcacgatgtg atttggggcg ccaattaccg cttatataac aacaagattt
     7801 ttgatacttt attaacaagc gctagtccgc gagaacggac gcaccaccaa tttagcggtt
     7861 ttattcgcga tgatgttgct ttaatacctg atcgtttatt attttcactc ggaactcggc
     7921 tcgatcacaa tgatttttcg ggattggaag ttcaacccaa tgcacgttta atgtggacac
     7981 ccaatgcgca gaattcgata tgggcatcaa tttcacgtgc agtccggatt ccttcgcgag
     8041 gggaaaatga tattactttg aatgcgggtc aacttcaaag tttgccagga atacctactt
     8101 tgcctatccc tatttctatt ctcatgcaag gagcaaacaa tttcggctca gaaaaactac
     8161 ttgcctatga gttaggctat cgccaccagt tttcccaaca agcatcgatc gatattgctg
     8221 gattcatcaa tgactatagc cacttacgcg acttaagctt tggggcgtta tcctttagca
     8281 caggattacc aaggcaatta atattaccgc tgctacttaa caatcgcgca tctgccctaa
     8341 cctatggctt tgaagtctct gcagattgga aacccacaaa tcgctggcga ctccaaggaa
     8401 attacagctt cttagatatc agcacttctt cgaacgaatt gctaaaaaca tttgacccaa
     8461 cgaccggtgg cgcggctaaa gttaatccgc aacatcaatt ctcagtgcgt tcaaactatg
     8521 atatttcaga taggttacag tttaaccttt ggctgcgata taccagtgca atcggcttct
     8581 acaatattcc aggtttcgta accatggata ctaaactcgc tttcaaaccc gtcaaagatg
     8641 ttgaattatt tctagttgga caaaacttat tcagtgaata tcacagagag tttgtatcag
     8701 ataatatccc atcgattcca gcactcatcc ctcgtggcat ctacgttggt gcccgatggc
     8761 agttctaact acctacctgt aagccactcc atctgcatgt ttaagcagta aagcgatatt
     8821 gattagtatc gctttgagtt ctaatggttt cccttaatga aaaatcaggg atcgcccaat
     8881 atacgcaagc caccctcttc ggtactatga cgctatcgaa acttttttca attctgcata
     8941 aggaaattta acatgaaaac acgtcatcta ctcgttctac ttgcttttgt tgcacaaagt
     9001 tttgctgtta acgctgaatc aacgattgct cctagcaatc atgaatcgct tgcacagcat
     9061 catttaagtc tagtcgatga agcaaaagta aaacttcaag agcacaaaaa taatcttgaa
     9121 gattatgagt tacatagcta ctactatggt agaggcggtc aagattttag atcacacgaa
     9181 acagccaatg ttcgtttgta tgaagagatc attgctgaga acctgaaaca agcagaattt
     9241 catcaacaaa tggctgaagc agaaaaaaat caaattaaca aagcaaacat taataatttt
     9301 gctgccggtg aagtttctag tgccgaatct actcattagt aacacatgaa tcatccccaa
     9361 aaaggatgat tcattcattg aaatgcgcgt taatttaagg agatcttgaa catgaaaaaa
     9421 tttttcttct ctgtactgat attaactttt ctagtaacaa atctatcggt cgctgctgag
     9481 tcggagaaca ccgctactgc taagaataaa tccccactga gtcatgagga aattgctcag
     9541 cgctatgata ataaagctaa gagaatgatc gacaaaattg cggaagaaaa acaaatgtta
     9601 gatgagtatg aacttcatag ttattactat ggccgtgaag gacaagattt tgcgtcgcat
     9661 catgaagcct tattacataa atttgagaaa gctgctaaaa gatatgaaga attagctgcg
     9721 gcgcaccgtg aaatggcaag aaaaacaaat tagcgcatca gcgctgcaaa gcagagagcg
     9781 ttactaagtt taatataaaa cttgaaaagg gtaacgctca tctctgtatc atctgtagtt
     9841 atcaatcatc aaatcaaaat atcatccaca ggcttaacga tatcgggttg atcagcatca
     9901 ttgatcaact ccagcaagtt aatttcaata ttccgagaaa tggcagtcaa ggcaagatca
     9961 ttcggctgca acccaaatgg actctcgatt tcatcaccaa tcgcatctaa ttcaaaaaaa
    10021 gcgtaggaaa tcatcaagac tacgaccggc gtcagaattc cagtcgtctc gactaaacca
    10081 aaaggtagcg ataaacaata agctgcgaca attcggtgag tcaatactga gtaactgaaa
    10141 gggattggag tgttcttaat gcgctcgcac ccccctaaaa tattcgagaa ttcattgagc
    10201 tgcgcttcta aaaacggatg attatattcg ctcaaccaac gacgatcgcg tgcaatggct
    10261 agatcacgac tcaattgctg caaaatgatt aagggtcggt gttttgcaac agcaaccgca
    10321 gcataatcat ctgcgggtaa aaattttttt atctcctcaa agggttcttg attgcgtaaa
    10381 tgacagcgta gcgcatgtgc aaatgcgatg attcgtttga caaacatggc ttgaaatggc
    10441 tgcaatgttt cgcaactctg tcttgaatca gtcatgtgac aaatttggcg tgtcaagcta
    10501 cgtgcagtat tcaccaatgc cccccataac ttacgccctt cccagaattt atcgtatgaa
    10561 gcactgttgc gaaaccctag aaaaatacct aacgcaaccc caaccaatag gaacggcgtt
    10621 gttgtgagcg tgtaagcttg aatactgtaa tagagctcaa tgtaggtcac aaccgatgct
    10681 gtcacagtaa ccgtgagtat tctcggccac gtttccataa agctactgcc gcgaaaatga
    10741 aacaacaagc gcacccaaga cactttatct ttaacaatca caactcaaat ctccttcgat
    10801 ttcatgctca catgttcatc ttgatttagc ctagcgcact ctaaattagc aaaaaccagc
    10861 catccttgtt ttctttaaag cgtaacagaa aaccagtttt agcgtgatcg ctaagctgtt
    10921 tcatcgtttg agacctaata aagatttgga tctcagcatt tttcaaagca tctcaaccaa
    10981 gaaatctaag ttagaatgct gggatatctt ctgcttttaa cccttcttaa tctgaaattt
    11041 tagttcatgg ataaaaattg aaaaaacgga tctgatgaac aaaaaacaac tttccgaacg
    11101 cgatatttgc accaaattca ttacacctgc gctggtgcgt gcgggttggg atatctcatc
    11161 gcaaatcaga gaagagtttc cgctcaccaa ggggcgcatc atcgtacgtg gtaaattgca
    11221 tactcgggct gaacgcaaac gcgcggacta cgtgttgttc tataaatcaa atatcccaat
    11281 cgccatcatt gaagcaaagg ataacaatca tacagtcggt gatggcatgc aacaggcgtt
    11341 gggttatgct gaaatgctgc aagttccgtt tgtgttcagc tccaacggcg acggtttctt
    11401 atttcacaat aaaatcgcac aagatggtat cgtcgaacgc gagcttgcgt tgcacgaatt
    11461 tcccgatgca gaaatgcttt ggcaatggtg ggtaaaacac ctaggcttga acaaccaaca
    11521 aaatagcctg gttactcagg actactacag cgatggtagc aataaaaacc cacgttatta
    11581 ccaattgctg gctatcaata aaactatcga agcaattgca cgtggacaga accgtatcct
    11641 actggtcatg gctgccggta ccggcaagac ctataccgcc tttcagatca tctggcgact
    11701 ttggaaatcc aaaacgaaaa aacgtatctt gtttcttgcc gaccgtaata tcttgattga
    11761 tcaaaccatg actaacgatt tcaagccctt cgggtcgtct atggccaaaa ttcagaatcg
    11821 tcaggtcaat aaatcttacg aaatttattt atcgctttat caagctgtaa ctggcactga
    11881 agaagagaaa aatatctata agcaattcag tcctaatttc tttgatctga ttgtgatcga
    11941 tgaatgtcac cgcggcagcg ctgctactga ttctgcttgg cgtgagattt tggagtattt
    12001 ttcttccgcc acgcaaatcg gtttgacagc tacacccaag gaaaccaaag acgtctccaa
    12061 tattgattac tttggtgacc cgatttatac ctattcactc aagcaaggta tcgacgatgg
    12121 tttcctagct ccctataaag tcgtgcgtat cgatctggat aaagatctga ctggctggcg
    12181 acctgataaa gggatgcttg ataaacatgg atttgaaatt gaggatcgca tttacaatca
    12241 acaagatttt gacaaaacgc ttgtgttgga aaaacgcaca cagatggtcg cgaaaaaaat
    12301 ctctgaattc ctcaaacaaa ccaatcgctt cgacaaaacc atcgtattct gtgaaaacat
    12361 cgatcatgca gagcgcatgc gtcaggcgct aatcaacgaa aatgctgatc ttgcagggca
    12421 gaacaacaaa tacgtcatgc gtatcaccgg agataacgaa gaaggtaaag ccgaactcga
    12481 caatttcatt tttccggaaa gcaaataccc cgtcattgcc accacctcga aactgatgac
    12541 aacgggtgtc gatgctcaaa cttgcaaatt gatcgtactc gatcaacgca ttcaatccat
    12601 gactgaattc aagcaaatca tcggccgcgg cactcgcatc aacgaagatt acggcaagta
    12661 ctttttcacc attctcgatt tcaaaaaagc caccgagcta ttcgctgatc cggattttga
    12721 cggcgacccc gtgcagattt atgaacctaa aggaactgaa tcccccgtgc caccggatat
    12781 ttcagttgaa ggagcaagcg gtgaaagtat gatttactcg agttctgaca caaatccgat
    12841 ttggaccggt gcggcgcaaa ctgacccaga caacgagact tatcgtgtcc gccgctttac
    12901 cgttgccaat gtcgaggtta aagttgttgc tgagcgagtc cagtattttg acgcgacggg
    12961 tcgattgatt accgaatcac tcaaagatta cacccgcaag gcggtagcca gagaatatgg
    13021 ctctctcgat gattttctac gccgctggaa cagcgctgat agaaaacagg tcatcattaa
    13081 tgaactatcc gagcaaggcg tgttcttcga tgcacttgcc gatgagatcg gacaacaatc
    13141 cggcaagctg ttcgatcctt tcgacttgct ctgtcatgtt gcgtgggaca tgccaccgct
    13201 cacgcgcaaa gaacgagttg agcaggtaaa aaaacgcaat tactttaccc gctatggtga
    13261 acaagctcag cgcgtgctgc aagccctgct cgataagtat gccgatgaag gcatggcaca
    13321 gatcgaagaa acgcaaatcc tcaccattgc cccttttacc ggattcggca caccaatgga
    13381 aatcatccgc gcctttggcg gtttggatca gtatcagcag gccgtgcatg acctagaaga
    13441 tgcgctttat cttcctgacc gtcattcccg cgaaagcggg aatccaggag ggtgatatga
    13501 agcaaccctg tgtctacatt ctcgcaagcc agaaaaacgg taccctttat accggcgtaa
    13561 caagtgatct gattaagcga gtatgggaac acaaacatga tcaggcagaa gggtttacca
    13621 gtaaatatgc ggtacatcac ctggtttact ttgaacagca tgaagacatg aatgccgcga
    13681 ttacccgcga aaagcagatc aaaaaatgga atcgggcttg gaagcttcgt ctgatcgaag
    13741 cactgaatcc gaattggaat gatttatacg aaaccttgat ttgaattttc tcgtcatcct
    13801 cccacaggcg gggacccaga agacaaaatt gctggattcc cgctccccat ctgcacgaga
    13861 acaggcatgt aggaatgaca agatgaatcg ccccgggttt tccggagata aaatgatttg
    13921 agaggaggag gaagaga
//
LOCUS       DAOQKQ010000058        13864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_2068_length_13864_cov_6.218698, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000058 DAOQKQ010000000
VERSION     DAOQKQ010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 13864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13864
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2068_length_13864_cov_6.218698"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="PKZ49_05340"
     CDS             <1..900
                     /locus_tag="PKZ49_05340"
                     /inference="COORDINATES: protein motif:HMM:NF040606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme-cytochrome C peroxidase"
                     /protein_id="HND36022.1"
                     /translation="LWVKHLRAPSWSDGNIKLPVIDQAKANIGERIYMEQCLDCHKII
                     PSNKQADPYKAKMISLSEVGTDSAMAINADYHMAKSLLLEGVKSNILIGDKFTATEPS
                     IELAVNGVTGLVLKDLTKAIKAGIVTDGGLLAEVDDRLLEPSLKKILTGIKSKNDILE
                     KYLEARKDLRKKLHAKLSELTKGSGSSVSPEPNLDELKYKARPLNGIWATAPYLHNGS
                     VPNLWELLKKPEERVTQFWVGSRELDPVHVGFEINQGLSQFNVHKVGTKTIIPGNSNL
                     GHDYGTDLSDDEKWSLIEYLKTL"
     gene            complement(967..1623)
                     /locus_tag="PKZ49_05345"
     CDS             complement(967..1623)
                     /locus_tag="PKZ49_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HND36023.1"
                     /translation="MSAQKAQVMLVDDHAMLRHGMAMLIDAEPDMQVIAEASDSVETL
                     AFLKKNSDVDIILVDISLKTISGLEVIKSIHLLKPELPVLVVSMHDESVYAERALHAG
                     ARGYVMKQEPGENLIIAIREVLNGNVFLSKAMQMKLLNRVASGNNKPEIAINNLTPSE
                     FEVLHLIGKGHNTQEIAKLLNRSIKTIETHRFNIRTKLNLKDSADLIRYATRWVLEEQ
                     "
     gene            complement(1625..3640)
                     /locus_tag="PKZ49_05350"
     CDS             complement(1625..3640)
                     /locus_tag="PKZ49_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HND36024.1"
                     /translation="MQLIRDDRKISIIGGLLLLCLTLATGMAVYNVMRQQIESTLGRG
                     LDVALQGKARLFESQISEGLAATRATVNRPFLIRSLEQIKEQPNNIRALQDLQRNVDS
                     LILADFTAASISNSDGKELARVGKFSSDHTPKLPISIRSSAWLYWNDGFVLSVTKDIL
                     DQAGTRVGSLTTEKSLPQLTESFSKIWEIGKTGEFIVCAPPNEASDQMHCLISQISGV
                     EFRFLSRVIRNEALPMDYALRDQRGVIASKDYRHIPVIAAYAPLESVELGMVLKLDEE
                     ELLKPMNEQLKVIILYISGLIITEILLLYWLVHKLIRAIRGSRKAKERAEQFSTELAH
                     KELALRERFKEITCLYEIGSNMKSEFSIQEICQLVFKHLIGAMQYPDDASAFIELDGI
                     QYMSDNFQCEGTHFLQSKIELDGKICGHLYVFYPYDKPFLAFEEQKLLDAVARDIESW
                     LARKRLEQALVSVAEENQRQIGQELHDNLGQQIAAIGYQAKALEKRMITAGDTASTAI
                     ATSIAAQSQSAVSQCRQLAQGLLPFELETNGLIAVLHAFAAKISTTYKIDCHFSTNSE
                     IIMNDDVALNLYRITQEAVNNAIRHGGAQHVDISLLIEKGKLWLSITDNGRGFIHNHA
                     SHDIKNIITPGIGIKIMRYRTKQIGATLEFLERPEGGVEVKVSRKLS"
     gene            complement(3793..4404)
                     /locus_tag="PKZ49_05355"
     CDS             complement(3793..4404)
                     /locus_tag="PKZ49_05355"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HND36025.1"
                     /translation="MQTLWYIVDPMCSWCWGFSPIIETIREIYKNQLQIELIVGGLRA
                     GTTEPLHPSKRSEILQHWQNVQHRTNQPFKFENALPEGFVYDTEPACRALVTAATIDS
                     ACIFALLHTIQNAFYAEQLDVTRTEILSQLAVNVGIQPERFIATYNSDTLKLKTQQQF
                     QQAMQWGVSGFPSLVYLKNNVPNFLMRGYCDLHELQTRITTVL"
     gene            complement(4454..6268)
                     /gene="typA"
                     /locus_tag="PKZ49_05360"
     CDS             complement(4454..6268)
                     /gene="typA"
                     /locus_tag="PKZ49_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HND36026.1"
                     /translation="MPHPLRNIAIIAHVDHGKTTMVDKLLHQAGTFAAHQTIAERVMD
                     SNDIERERGITIFSKNCAVDYEGVHINIVDTPGHADFGGEVERVLSMVDGVLLLVDAV
                     EGPMPQTRFVTKKALALGLRPIVVINKVDRPGARPDWVINHTFDLFDKLNATEAQLDF
                     PVVYASALNGYATTDLTQPSTDMRPLFDTILKHVPSPGGNPDEPLQLQISALDYSSFV
                     GRIGVGRITRGRLKPAQEVMIMTGNQTPKKAKVNQVFGFQGLERVPLSEALAGDIVQI
                     NGIEELSIGTTLADIDHPEALPMPKVDEPTLSMTFQVNTSPFAGKEGKFVTSRQIRER
                     LEKELLTNVALKLEETTDTDSFLVSGRGELHLTILIENMRREGYELAVSRPRVVIREI
                     DGVKCEPFEVLTVDVDEENQGAVMEALGARRGDLLDMASDSRGRVRLDYRIPARGLIG
                     FQSDFMTMTRGTGMMSHVFDDYGPMRPEIAARRNGVLISAEHGEAVAYALWKLQDRGR
                     MFVNPGDPVYEGMVIGIHSRENDLVVNPIKGKQLTNIRASGHDEAVALIPPIQLTLES
                     AVEFIADDELAEITPKSIRIRKRHLQEHERKRAARSAA"
     gene            complement(6674..7393)
                     /locus_tag="PKZ49_05365"
     CDS             complement(6674..7393)
                     /locus_tag="PKZ49_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HND36027.1"
                     /translation="MMRLRSLMILLLLSCLISQALADETQLRKLLEDHFPGTEIESLR
                     KTPFAGLYEVVVGGEVLYTDDQAKYFFMGHMIDTQSRVSLTSERMQQIRDARRIPIDT
                     LPLEHSIKVVKGKGERTLIVYSDPNCPFCKKLEKELVDIKNITVHTLLYPILNGSMPT
                     ATSIWCASDRLKAWDDFMLHGKAPASKECETPIQALLQSGQKYRVTGTPTLIFADGSV
                     VSGMIPAAEIEKRLEQAKKPK"
     gene            complement(7447..8616)
                     /locus_tag="PKZ49_05370"
     CDS             complement(7447..8616)
                     /locus_tag="PKZ49_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF family hydroxylase"
                     /protein_id="HND36028.1"
                     /translation="MKFDIVIIGGGLVGASLATSLKGSHLTVALIDAAAPTPLPNDAS
                     WDNRIYTISPGSADFLRSLGLWQQIDASRVTPIYEMNVFGDDGAAEIDFGAYESGLPE
                     LAFVTENRQLQAVLWGALLNDSQNITVFSSAKCTAISWHDSGVNILLADGRTIQASLI
                     VGADGVNSWVREQAGITVEHHRYEQIGVVANFSTELAHHNIAYQWFRRDGVLAFLPLP
                     DKRVSIVWSTHETHANELRNISDEEFSQRVSEASNRVLGRLQLITQPAGFPLNFVHVS
                     KLVKPRLVLIGDAAHGIHPLAGQGVNLGLRDVKALSEILKKDEIYYDYGDYILLLRYE
                     RARKADITAMETVTDGLHKLFNSQDPIIRRFRNLGLTMTNRLPFIKSKLMQHALN"
     gene            8748..8823
                     /locus_tag="PKZ49_05375"
     tRNA            8748..8823
                     /locus_tag="PKZ49_05375"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8781..8783,aa:Met,seq:cat)
     gene            9175..9423
                     /locus_tag="PKZ49_05380"
     CDS             9175..9423
                     /locus_tag="PKZ49_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36029.1"
                     /translation="MKLQHFIVMISLLIFSSSAFAYRCVIDMRKIDEALSKQPAITEM
                     QEQEIRKLRAEGEALHNKGKHKESVEALHKALEMLGVR"
     gene            9449..10603
                     /locus_tag="PKZ49_05385"
     CDS             9449..10603
                     /locus_tag="PKZ49_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HND36030.1"
                     /translation="MPAIYFDHNATTAVDEKVLEAMLPFFRQKYGNASSLHSYGIEAR
                     RAINQAREQVAHAVGVQSSQVIFTSGGSEANNLFIRGAADYLKPANIVISAIEHPCVR
                     RPAQELARRSNNPWTLHQLTVNTEGQVNLTDARNVISDKTGLVSVMLANNETGVIQDV
                     AAIAELAKNQGAWMHTDAIQALGKIVLNFADLNVHAMTISAHKIYGPKGAAALIVDKR
                     LILKPLIYGGGHENGMRSGTENVPAIVGFGVACELVKARISEASAHIARLREYLEQGL
                     SALGATIFGQNVPRLPNTCYFAIPDIEGDTLVVKLDKAGFAVASGAACSSATPGKSHV
                     LEAMGVAPMLARCAVRISLGHDNTLNQIDDFLKAINKIVAALRQFNSMSA"
     gene            10721..11533
                     /locus_tag="PKZ49_05390"
     CDS             10721..11533
                     /locus_tag="PKZ49_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HND36031.1"
                     /translation="MMNSNPTKAIILSAGQGRRLLPLTENTPKCLLSVADKPVIAWQI
                     DALIDAGIDSITIVGGFQIGKVEALIAQRYAAYPEIRVCFNPFYEVADNLASCWIVRD
                     QMEGNFVLINGDTLFETTLVAHVLQSEYAPITLSVDYKETFDEDDMKVELNSDKWVRH
                     VSKLLVPEQSHAESIGFIYFQQEGAILFRNAVEQALRDPSKLKSWYLSIIDQLAEQHQ
                     VNSRSVQGFKWGEIDFIEDLQRAEKLFSTLDNKLNRYQQESSLRETIIPLPM"
     gene            11535..11987
                     /locus_tag="PKZ49_05395"
     CDS             11535..11987
                     /locus_tag="PKZ49_05395"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HND36032.1"
                     /translation="MALTVFNGTMQKFGAPDTIIRQYQHWSVLLRPAQLTLGALVLVA
                     HEPVFAFSELSSASFSELHQVTREIDSALKNAFGYDKLNYLMLMMVDPDVHFHVIPRY
                     EAARSFNGQDFFDAGWPGVPNFSQVNQTDRVINQLIIDHITKHWKSTL"
     gene            11989..13032
                     /locus_tag="PKZ49_05400"
     CDS             11989..13032
                     /locus_tag="PKZ49_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HND36033.1"
                     /translation="MSDRSSVDFPLREAYALVSDLMTPDPRIYWVDFLFHILLGWTGF
                     IVTLQTTLFSIEQILGFVITVFTFYRSAIFVHELAHLKKGTFQVFRWVWNLICGIPMM
                     IPSFMYDGVHNDHHKRDVYGTHLDGEYLPFATQKPIEMVRYVLLSFILPLLLVARFLL
                     LTPLSYLIPPLRKLVWQRASSLTINPQYIRAPNAIRNDHYWWLQEFATFAFAATVVGL
                     VIVGLLPYQALVLWYVTAVCVFILNSLRTLAAHAYRNPGDHSLELAEQYLDSINIPGN
                     FLTTLWAPVGLRFHATHHLFMSLPYHNLGKAQQRLVNGLSDNSLYLKTIRKGLWDALK
                     RIWKEASIASISR"
     gene            13043..13750
                     /locus_tag="PKZ49_05405"
     CDS             13043..13750
                     /locus_tag="PKZ49_05405"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HND36034.1"
                     /translation="MNNPSRPIQLVLLRHGQSIWNQDRHFTGWSDVALSEKGKEEAKR
                     AAHLLKQANFSFDLCYSSVLERAVDTLNIIRAEMGLERAPLIQDWRLNERHYGALEGL
                     QRWEAIKKFGIWPVLACQIFYKAMPPILELNDLRFPGNQLRYQSINQQLLPRTESMEQ
                     TLARVYPCWQNDILPEIKRGKRILIVSHRNLLRTLVMQLNHLSPWHVMKLSIATGKPL
                     CFELDDQLNSIRSYYLQ"
BASE COUNT         3888 a         2887 c         2996 g         4093 t
ORIGIN      
        1 ctatgggtta agcatttacg tgcccccagc tggtccgatg gcaatataaa attgccggtt
       61 attgatcaag ctaaggcaaa tatcggtgag cgaatctata tggagcagtg tctagattgc
      121 cataagatca ttcctagcaa taagcaagcg gatccttata aagcaaaaat gatctcttta
      181 agtgaagttg ggacggattc cgcaatggcc atcaatgctg attatcatat ggctaaatct
      241 ttattgctag agggagttaa atccaatatt ttgattggag ataaatttac tgcgaccgaa
      301 ccatcgattg aattggctgt taatggcgta actggattgg tactaaaaga tttaaccaaa
      361 gcaattaaag ctgggattgt taccgatggc ggtcttttag cagaagttga tgataggtta
      421 ctagagccga gcctaaaaaa aatcttgact ggcattaagt ctaaaaatga tattttggaa
      481 aaatatttag aggctcgtaa agatttacgt aagaaattac atgccaaact gagtgagctt
      541 acaaaaggat cgggttcatc ggtttcgccc gagccgaacc ttgatgaact taaatataag
      601 gcgcgcccgc ttaatggtat ctgggccact gcaccctatc ttcataatgg ctctgtccct
      661 aatttatggg aattactgaa aaaacctgaa gaacgagtaa ctcagttttg ggtcggtagt
      721 cgagagctcg atcctgtcca tgttggtttt gaaatcaatc aggggctttc gcaattcaat
      781 gttcataaag tgggaacaaa aacgatcata ccaggtaact ctaatttggg gcatgattat
      841 ggaaccgact tgtctgatga tgaaaagtgg tctttgattg aatatctgaa aacactttag
      901 caagtgatac gatactgtca gaaaatttga tctttgatag atagggtttt gggggagcga
      961 gaaaagttat tgttcttcca aaacccatcg cgtagcgtag cgaatcaagt ctgcgctatc
     1021 ttttaggttt aatttggtac gaatattgaa tcgatgggtt tcaatcgttt taatgctacg
     1081 attgagtaac ttggcgattt cttgtgtatt gtggcctttt ccaattaaat gcaacacttc
     1141 gaactcactc ggcgtaaggt tattaattgc tatttctggc ttattgtttc ctgaagccac
     1201 tcgattcaat agtttcattt gcatggcctt acttaggaac acattaccgt ttaaaacttc
     1261 tcggatcgca ataattaaat tttcaccggg ctcttgtttc atgacgtaac ctcgtgcgcc
     1321 tgcatgtaaa gcacgttcag catagacgga ttcatcatgc attgaaacaa cgagtaccgg
     1381 tagctctggc ttcagtaagt gaatactttt aatgacttcc aggccagaaa tcgttttaag
     1441 ggagatgtcg accaagataa tgtctacatc agaatttttc ttgaggaaag ctaatgtttc
     1501 cacactatcg cttgcttccg caataacctg catatcaggt tctgcatcaa tgagcatagc
     1561 cataccatga cgtaacatag catgatcatc gacaagcatg acttgtgctt tttgagctga
     1621 catattaact aagctttctc gagactttca cttctactcc cccttctggc cgttctagaa
     1681 attctaatgt tgcaccgatt tgcttcgtgc gatagcgcat aattttaatg ccaatacctg
     1741 gcgttataat attctttata tcatgactgg catggttatg gataaaacct cgcccattat
     1801 cggtgataga tagccataac tttccttttt cgataaggag tgaaatatct acatgttgcg
     1861 ctccaccatg ccggattgca ttgttgaccg cttcttgtgt gattcgatag agattaagcg
     1921 cgacatcgtc attcatgata atctcactat ttgtggagaa gtggcagtca attttgtaag
     1981 tcgtacttat ttttgcagca aatgcgtgaa gtacggcaat tagcccattt gtttccaact
     2041 caaaaggtag taaaccttgt gcgagttgtc tgcattgcga gacagccgat tgtgattgag
     2101 ctgcaatgct agttgcaatt gcagtagaag cagtatcacc agcagtaatc attctttttt
     2161 caagtgcttt tgcttggtaa ccgatggctg cgatttgttg accaagatta tcatgcaatt
     2221 cttgtcctat ctgtcgctga ttttcctcag caactgatac taaagcttgt tccaggcgct
     2281 tacgcgcaag ccaactttcg atatctcttg cgacggcatc aagcaatttt tgttcttcaa
     2341 atgctaaaaa tggcttatcg taaggataaa aaacgtataa gtgaccgcag atttttccgt
     2401 ctaactcaat ttttgattgc agaaaatggg taccttcgca ttgaaagtta tctgacatat
     2461 attgaatacc atctaactca atgaatgcag aggcatcatc tggatattgc atcgccccga
     2521 tcaaatgctt gaaaactaat tgacagattt cttggatcga aaattctgat ttcatgttac
     2581 tgccgatttc ataaagacaa gtaatttcct taaaccgttc acgtaatgca agttctttgt
     2641 gagccagttc agtagaaaac tgctccgctc tttctttggc tttccgcgat ccacgtatcg
     2701 ctctgataag tttatgaaca agccaataaa gcaataaaat ctcagtaatg atcaatccag
     2761 aaatatacaa gatgatgact ttaagctgct cgttcattgg tttaagcagt tcttcttcat
     2821 caagctttag caccattcca agttcaaccg attctaatgg tgcgtatgct gcaatgacag
     2881 gaatatgacg gtagtctttt gaagcgataa cccctctttg gtcgcgtagc gcataatcca
     2941 tcgggagtgc ttcattacgg atgacacggg acaggaatct aaattctacc cctgatattt
     3001 gactaatgag gcaatgcatt tgatctgatg cttcattagg tggcgcacaa acaataaact
     3061 cccctgtttt accaatttcc catatcttgc taaaactttc agttaattga ggtaggcttt
     3121 tttcagttgt aagactgcca actcttgtcc cagcttggtc gagaatatct ttggttacgc
     3181 tgagtacaaa tccatcattc cagtatagcc atgcgctaga tcgtatgcta atgggtaatt
     3241 taggtgtgtg atccgatgag aacttgccga cacgggctaa ttccttgcca tcgctatttg
     3301 aaatcgatgc cgctgtaaaa tcagcgagta ttaatgaatc cacattgcgt tgaagatctt
     3361 gtagtgctct aatgttattg ggttgttcct taatttgctc aagcgagcga atcagaaagg
     3421 ggcgatttac agtagcacgg gtggctgcta ggccctcaga aatttgactt tcaaacaaac
     3481 gggccttacc ttgcaaagca acatcaagcc ctcgtcccaa ggtagattca atttgttgac
     3541 gcatcacgtt gtaaacagcc attccagtgg ctaatgtcaa gcagagtagg agtaggcctc
     3601 ctatgatgct gatcttgcga tcatctcgta ttagctgcaa atcattctcc tatcaaatag
     3661 tctgatccta aagtcatcgc tgatcgcttg ttgcgtatga gattatagga gtaaatgatc
     3721 tggggttgtt acaacctatt ttaggagttg ggtaaaaacc tagaattcaa ttcaaatttt
     3781 taatgcttca cattacagca ctgtcgtgat gcgcgtttgc aattcatgga gatcacagta
     3841 gccacgcata agaaaattag ggacattatt tttcaaatat actaaagaag gaaatccgct
     3901 tacaccccat tgcatggctt gctggaattg ttgctgtgtt ttaagtttga gtgtatctga
     3961 gttgtaggtg gcgataaatc gctcgggttg aatgcctaca ttaacagcga gctgactcag
     4021 tatttctgtg cgagtaacat caagctgctc agcgtagaag gcattttgaa tcgtatgtag
     4081 taacgcaaaa atacaggctg aatcaatggt tgccgcagtg actaacgctc ggcaagcagg
     4141 ttctgtatcg taaacgaaac cttcaggcag agcattttca aatttaaagg gctgattggt
     4201 cctatgttga acattttgcc agtgctgaag tatttcagaa cgtttgctag gatgaagtgg
     4261 ttctgtagta cccgcacgta atccaccgac gattaattct atttgaagct gatttttata
     4321 gatttccctg atggtttcga taataggtga aaacccccag caccaggaac acatagggtc
     4381 aacgatgtac cacagcgttt gcatcaagaa gtacttagga tgtaaatccg ctgtggctta
     4441 tatctgctag cgactaagcc gctgagcgtg cagcacgttt acgctcatgt tcttgcaagt
     4501 ggcgtttacg aattcgaata ctcttgggtg taatttctgc taattcatca tcagcaatga
     4561 attcaacagc cgattcaagc gtgagttgaa tggggggtat taaagccacg gcttcatcat
     4621 gtccagaagc gcggatattg gttaattgct ttcctttgat cgggtttacc acgagatcat
     4681 tttcacgact atgaattcca ataaccatgc cttcataaac gggatcgcca ggattaacga
     4741 acatacgtcc ccgatcctgt agcttccata gagcataagc taccgcttcg ccatgctcgg
     4801 cagaaattag gaccccattt cttcgggccg caatttctgg gcgcatgggt ccataatcgt
     4861 caaatacatg actcatcatg ccggttcctc gcgtcatcgt catgaaatct gattgaaaac
     4921 cgatcaggcc gcgggcggga atacgataat cgagcctgac tcgcccacga ctatcagatg
     4981 ccatatcaag aagatcacca cgacgagcgc ctaatgcttc cattacagcg ccctgatttt
     5041 cttcgtcgac atcaacggtg agtacctcga agggttcaca tttaacccca tctatttctc
     5101 gaataacgac gcgaggacgt gataccgcta attcataacc ctcacggcgc atattttcga
     5161 taagaatggt taagtgtaat tcaccacgtc ctgaaactaa gaatgaatcg gtatcggtag
     5221 tttcttctaa tttcagtgct acattcgtca gtaattcttt ttcaagtcgt tcacgaattt
     5281 gtcggctcgt gacaaatttt ccttcttttc cggcaaacgg cgaggtgttg acctgaaatg
     5341 tcatcgacag cgttggttca tctactttgg gcatgggtaa cgcttcaggg tgatcaatat
     5401 ccgcgagcgt ggtaccgatg ctaagttctt caatgccatt gatttggacg atgtcaccag
     5461 ctaacgcttc gctaagcggt acacgctcca aaccttgaaa cccaaagact tggttcactt
     5521 ttgctttttt cggtgtttgg ttaccagtca tgatcatcac ttcttgcgca ggtttaaggc
     5581 gaccacgcgt aatccgacca acaccaattc gaccgacgaa gctcgagtag tccaatgcac
     5641 tgatttgaag ttgcaagggt tcatcaggat tgccacctgg actcgggaca tgcttgagga
     5701 tagtatcaaa taaaggccgc atgtcggtac ttggttgagt aagatctgtg gtggcataac
     5761 cgttcagcgc tgaagcatag accacaggaa aatcgagctg agcctcggta gcattgagtt
     5821 tgtcaaacaa gtcgaaggta tgattgatga cccaatctgg gcgcgcgcca ggtcggtcaa
     5881 ctttgttaat cacgacgata ggtctcaatc ctagcgcaag cgcctttttt gttacaaaac
     5941 gcgtttgtgg catcgggcct tctaccgcat cgacgagcag caaaacacca tccaccatcg
     6001 agagaacgcg ctccacttca ccaccaaaat cagcatgacc gggcgtatcg acgatattga
     6061 tatgcacgcc ttcgtaatca acagcacagt ttttggaaaa aatggtaatg ccacgctccc
     6121 gctcaatatc attggaatcc atgacacgct cagcaatggt ttggtgagcg gcaaaggttc
     6181 ccgcctgatg gagcagttta tcaaccatgg tagttttgcc atgatcgaca tgggcaataa
     6241 tcgcaatatt ccgaagtgga tgtggcatgt caaaaatctc tgaatgttaa ttagaatgat
     6301 ttatacgtga agggggcgaa ttataacagt agatcaatta catagctagc ttggttattt
     6361 ctcaatctcg ttatcgatat taaattttat cctttaatct ttttaaatag tgttgaggtg
     6421 ttgggcccaa tttttggaaa taggatgtaa cctgccgcac ccgaaaagca gatttctggt
     6481 ggaagtaaat ttaattcaat aactaagaag cgtagaaatt gaatagacaa ccaaacactg
     6541 tgttattgta gttttaagct aaccttccca agcgctttac caatcatcga atgattacgg
     6601 aaagataggg ttatttctct taaccagaaa taatcgattt caggtgagga ttatttctgg
     6661 ttgtatattt tgcttatttt ggtttcttag cctgctctag gcgcttctcg atttctgctg
     6721 ctggaatcat tcctgatacc accgatccat cagcaaaaat gagcgtaggc gtgccagtca
     6781 ctcgatattt ctggcccgat tggagcaaag cctgaatcgg tgtttcacat tctttgctgg
     6841 ctggtgcttt accatgcaac ataaaatcat cccaagcttt caaccgatct gatgcgcacc
     6901 aaatcgatgt ggccgttggc atcgagccat ttaagatcgg ataaagcagc gtatggacgg
     6961 taatgttttt gatatctacc agctcttttt caagcttttt gcagaagggg caattgggat
     7021 cggaatacac aatcaaagta cgctcacctt ttccttttac gaccttgatc gaatgttcaa
     7081 gggggagcgt gtcaattgga attcgtcgcg catctctgat ttgctgcata cgctcgcttg
     7141 ttaggctaac tcgcgattgc gtatcaatca tgtggcccat gaaaaaatat tttgcctgat
     7201 catcggtata gagaacttct ccaccgacaa ctacttcata taggcctgca aaaggtgttt
     7261 tacgtaagct ttcgatttct gttcctggaa aatgatcttc gagtaatttc ctaagctgcg
     7321 tttcatctgc taatgcctga ctgatcaaac aacttagcag caataatatc atcaatgatc
     7381 gtaaccgcat catggtaata actcctattt attatggaag aatgggaaaa taatcagagc
     7441 acaagcttaa ttgagtgcgt gttgcatgag tttgcttttg ataaaaggta ggcgattggt
     7501 catcgtaagg ccaagattac gaaaacgcct gattattggg tcctgactgt taaatagttt
     7561 atgtaggccg tctgtgactg tttccatcgc tgtgatatct gctttgcgtg cccgctcata
     7621 tcggaggagt agaatataat caccatagtc gtaatagatc tcatctttct tcaagatttc
     7681 cgacaatgct tttacatcac gcaaacccag attaacccct tggccagcga gtggatgaat
     7741 gccgtgtgca gcatcaccaa tcagcactaa tctcggtttc acgagcttgc ttacatgaac
     7801 aaaatttaaa ggaaaacccg caggctgtgt gatgagttgt aacctaccca aaacacgatt
     7861 ggaagcctct gaaactcgct gggaaaattc ttcatctgat atgttacgta attcatttgc
     7921 gtgggtctca tgagtagacc agactatcga cacgcgctta tcaggaagcg gtagaaacgc
     7981 aagaacccca tctcttctga accactgata ggcaatatta tgatgtgcaa gttcagtgct
     8041 aaaattagcg actacaccga tttgttcata acgatgatgc tccacagtta ttccagcttg
     8101 ttcacgaacc caggaattaa cgccgtcagc accgacaatc aaactggctt gaattgtgcg
     8161 cccgtcagca agcagtatgt ttacaccaga atcgtgccag gaaattgccg tacatttcgc
     8221 agaggaaaag accgtaatat tttggctatc gtttaataaa gcgccccata gaactgcctg
     8281 taattgccta ttctcggtga caaacgctaa ctccggtaaa ccactttcat aggccccaaa
     8341 gtcaatttct gccgcaccat catcaccaaa tacattcatt tcgtagatcg gtgtgacacg
     8401 tgatgcgtca atttgctgcc aaagacctaa agaccgcaag aaatccgcac tccctgggct
     8461 aatggtataa atccgattat cccagctcgc atcattcggt aaaggtgtgg gtgccgcagc
     8521 atcgatcaaa gctaccgtta agtgactacc ttttaacgaa gtagccaaac tggccccgac
     8581 tagcccacct cctataatca caatatcaaa tttcatactg ggattataca gagaggtcgg
     8641 gtactaagaa ataaacgaat gaaattgtta ttgaattgat catctttttg ccaatcagta
     8701 ttgacagcat tctaactcca aaggcaaaat gcaggtctta cctttttggc gaattagctc
     8761 agtggttaga gcagcggaat cataatccgt tggtccgggg ttcaaatccc tgattcgcca
     8821 ccaattcaaa ataaaagaag aatgatcaaa gaactgagaa tcgcgttctt gatcgatact
     8881 tcttaacgaa atacaaattt gggttttaaa atctttcatt ttgactcggg atttaaataa
     8941 aaaatttaac ctccaaagtg attcagtatt tatagcctaa aaaccttttt ataattggct
     9001 ttccgaataa tttagtattt ttagcgattg gccttataag acgcttgtat acagaaaaaa
     9061 tataaaacaa tttatacggc tgctttctta actgtaaagt aagatcttat gctaactttt
     9121 aatgatcaat cgagtcatac ttaagttact aatttaaagc cataggagga tcaaatgaaa
     9181 ctacagcatt ttatcgtcat gattagcttg cttatattta gtagcagcgc gtttgcctat
     9241 cgttgtgtta tcgatatgcg aaaaattgat gaagctttat caaaacagcc tgcaatcact
     9301 gaaatgcaag aacaagaaat tagaaaatta cgtgctgaag gcgaggcgct ccacaataaa
     9361 ggtaaacata aagaatcggt cgaagcgctg cataaagctt tagaaatgct aggtgtccgt
     9421 taaatattta aagcgataaa acgagccaat gcccgccatt tattttgatc acaacgcgac
     9481 gactgcggtt gatgaaaaag tattggaagc catgctgcct tttttccgac agaagtatgg
     9541 gaatgcatcg agtttgcaca gttacggtat tgaagcacga cgcgcgatca atcaggcacg
     9601 tgaacaagtt gctcacgccg taggggttca gtcttcgcaa gttattttta ccagcggcgg
     9661 ttccgaagcg aataacctgt ttattcgcgg cgcagcagat tatttgaaac ccgctaacat
     9721 cgtcattagc gccatcgagc acccttgcgt caggagacca gcccaggaat tagcgcgcag
     9781 atcgaataac ccttggacat tacatcaatt aactgtcaat acagaaggcc aagtcaattt
     9841 aactgatgcg cgtaatgtaa tttccgacaa gacaggattg gtatccgtca tgctagcaaa
     9901 taatgaaaca ggggttatcc aagatgttgc agcaattgct gagctagcaa aaaaccaggg
     9961 agcttggatg cataccgatg cgatacaagc tttgggtaag atagtgctta attttgctga
    10021 ccttaatgtc catgcaatga cgatctcagc gcataaaatc tatggcccca aaggagcggc
    10081 agcgctcatc gttgataaac gccttatcct aaaaccgtta atttatggcg gcgggcacga
    10141 aaatggcatg cgttcgggaa ctgaaaatgt gcctgcgatt gttggttttg gcgttgcttg
    10201 cgagttggtt aaagcgagaa taagcgaagc ttctgctcac attgctcgac ttcgcgaata
    10261 tttggagcaa gggttaagcg cattaggtgc aacgattttt gggcaaaatg ttccccgatt
    10321 accaaatacc tgttatttcg ctatacctga tatcgaagga gatacgctag tcgtcaagtt
    10381 agataaggct ggttttgccg ttgccagtgg cgctgcttgt tctagtgcta cccctggtaa
    10441 aagtcatgta ctggaagcaa tgggagttgc gccgatgtta gcgcgatgcg cggtacggat
    10501 tagtttgggg catgataaca cgttgaacca gattgatgat tttctaaaag cgattaataa
    10561 aatcgttgca gcgctacgcc agtttaattc gatgagtgcg tgatagaatt aacagattga
    10621 cttcgtttac ggttatgttg tttttttcaa gtagaatagc aaccatttaa cccgagtgtt
    10681 tcatagtcat aaccgaatag attggagcat gacgcatacc atgatgaatt caaatccgac
    10741 taaagcaatc atattaagtg cgggacaagg cagaagactg ttaccgctca cagaaaatac
    10801 tcccaagtgc ttattatctg ttgcggataa acctgttatt gcttggcaaa ttgatgcatt
    10861 gattgatgct ggtatcgatt caataacgat tgtaggtggg tttcaaatcg gaaaggtcga
    10921 agcattgatt gctcaacgat atgctgctta tccagagatt cgtgtttgct ttaatccatt
    10981 ttatgaagta gcagataatc tggccagttg ctggattgta cgcgaccaga tggaaggtaa
    11041 ttttgtcctt atcaatggcg atacgctatt tgaaactact ttggttgccc atgttcttca
    11101 gtctgaatac gcacccataa cgctcagtgt tgattataaa gaaacatttg atgaagatga
    11161 tatgaaagtg gagctcaata gtgacaaatg ggttcggcat gtcagtaaat tactggtgcc
    11221 ggaacaatct catgcagaat cgattggctt tatttatttc cagcaagaag gagcaatact
    11281 gtttcggaat gcggttgagc aagcgcttcg cgatcccagt aaacttaaat catggtattt
    11341 gagtattatc gatcagcttg ctgagcagca tcaggttaat tcgagatcgg ttcaaggatt
    11401 taaatggggc gaaatcgatt tcatcgaaga tctacaacgc gcagaaaagc tattctctac
    11461 ccttgacaat aagcttaatc gttatcagca agaatcctcc cttcgcgaga cgattattcc
    11521 gcttccgatg taatatggca ttgactgtgt ttaatggtac gatgcagaaa tttggtgcgc
    11581 cggataccat cattcgccaa tatcagcatt ggagtgtttt attgcgccct gcgcaattaa
    11641 ctttgggtgc gttggtgctt gttgctcatg agccggtctt tgcattctct gaattaagtt
    11701 cagctagctt cagtgaatta caccaagtaa ccagagaaat tgatagtgca ctgaaaaatg
    11761 cttttggtta cgataaatta aattatctta tgttgatgat ggtggatcct gatgttcact
    11821 tccatgttat tccccgctat gaagctgcaa gatcatttaa tgggcaagat ttctttgatg
    11881 caggatggcc tggtgttccc aattttagcc aagttaacca gaccgatcgt gtcatcaatc
    11941 agttaatcat cgaccacatt accaagcatt ggaaatcaac tttataagat gtctgatcgt
    12001 tcctctgtcg attttccgct ccgtgaagca tatgcactcg tcagtgattt aatgacgcct
    12061 gacccaagaa tctattgggt tgattttttg tttcatatcc tactgggatg gacaggattt
    12121 atcgttacgt tgcaaacaac gcttttttca attgagcaaa ttttgggatt tgtgatcacg
    12181 gtttttacct tttaccgctc tgcaattttt gttcatgaac ttgcacatct taaaaagggg
    12241 actttccaag ttttccgatg ggtatggaac ttaatctgtg gcattccgat gatgattcct
    12301 tccttcatgt atgatggtgt gcataatgat caccataagc gcgacgtcta tggaactcat
    12361 ttggatgggg aatatcttcc attcgccacg cagaagccca tcgaaatggt tcgctatgta
    12421 ttactctcct ttattcttcc gctattgctg gttgcgcgtt tcttattgtt aaccccgcta
    12481 tcttatttga ttccgccgct gcgtaagctc gtttggcagc gtgcctcatc attaacgatt
    12541 aatccccagt atattcgggc acccaatgca attcgtaatg atcattattg gtggttacag
    12601 gaatttgcaa catttgcatt tgctgcaacc gtcgtagggc tggttattgt agggttactc
    12661 ccctaccaag ctttagtttt atggtacgtg actgcggtat gtgtttttat tcttaattcg
    12721 ttaagaacac tggctgcaca tgcttatcgt aatccaggag atcattcgct tgagttggca
    12781 gagcaatacc tagattcaat caatattcca ggaaattttc tgactacgct ttgggctcct
    12841 gttggattaa ggttccatgc aacccaccat ctttttatga gtttgcccta ccataattta
    12901 ggtaaagcgc agcagcgatt agttaatggg ttatcggata actcgcttta tttgaaaaca
    12961 atccgtaagg gattatggga cgcattgaag cgaatttgga aagaagcatc cattgcttct
    13021 atttcccgat aatgttgtta atgtgaacaa cccttccagg ccgatacaac tagttttgct
    13081 ccgccatggg cagagtatct ggaatcaaga taggcatttc acaggttgga gtgatgttgc
    13141 attaagcgag aaaggcaagg aggaagccaa gcgtgccgca cacttgctta aacaagccaa
    13201 ttttagtttt gacctttgct atagctctgt attggaacga gcggttgata ccctcaacat
    13261 tatacgcgct gaaatggggt tagaaagagc accacttatt caagattggc gcttaaatga
    13321 acggcactat ggcgcacttg aagggcttca acgatgggaa gcgattaaaa aattcggtat
    13381 ttggcctgtc ttggcttgtc aaatcttcta caaagcaatg ccgccgatac ttgaattgaa
    13441 cgatcttcgc ttcccaggga atcagttacg ctatcaaagt attaaccaac agttattacc
    13501 acgaactgaa agtatggagc aaaccctggc gcgtgtttat ccgtgttggc agaacgatat
    13561 tctgccggaa attaagcgag gtaaacgaat actcattgtt tcacatcgaa atcttttacg
    13621 cacgctggta atgcaattaa atcacttatc accgtggcac gttatgaagc tctctattgc
    13681 gaccgggaag ccactctgtt ttgagttaga tgatcagtta aactctatca gaagctatta
    13741 ccttcagtaa taaggggtca attcagaata aggttgtatt gggtaaaaaa ttactgagtt
    13801 gtaagaacgc cgtcaaccag atgcaaaatg cgttctgctc gctgagcgag ctgtgaatca
    13861 tgtg
//
LOCUS       DAOQKQ010000059        13832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_2080_length_13832_cov_6.210423, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000059 DAOQKQ010000000
VERSION     DAOQKQ010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 13832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13832
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2080_length_13832_cov_6.210423"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..389
                     /locus_tag="PKZ49_05410"
                     /pseudo
     CDS             1..389
                     /locus_tag="PKZ49_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611289.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            574..1434
                     /gene="motA"
                     /locus_tag="PKZ49_05415"
     CDS             574..1434
                     /gene="motA"
                     /locus_tag="PKZ49_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HND36035.1"
                     /translation="MLVSVGYLIVIVSVFGGFALAGGHLVSLFQPVELLMIGGGALGA
                     FFVGNSGKAIKATLKALPHVFKGAKYTKDVYMELMTLLYEILGKIRKEGLMAIEADVE
                     DPHKSPLFSKYPKILSDHHVIEFITDYLRLMVGGNLNPLEIENLMDGEIETHHQEEEV
                     PIHAIAKLGDGLPAFGIVAAVMGVVHTMESVGIPPAELGKLIAAALVGTFLGILLAYG
                     FVGPLASKLEQRLHESSKLLECIKVTLLANLNGYAPALAIEFGRKVLYSTERPSFAEL
                     EKHVKQSKAK"
     gene            1471..2409
                     /gene="motB"
                     /locus_tag="PKZ49_05420"
     CDS             1471..2409
                     /gene="motB"
                     /locus_tag="PKZ49_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HND36036.1"
                     /translation="MSDDLSQKPIIIKRIKKVSGGHHGGAWKIAYADFVTAMMAFFLL
                     MWLLGSTTQGDKEGIAEYFKTPLKIALAGGAGSGDSSSIIKGGGEDLSRRMGQVKRGD
                     IEEIKRTINLEAAQAEKQRLEIEQFKNLKKRIEQVIEANPSLINFKKQLLLDITTDGL
                     RIQIIDEKNRPMFSIGKAELQSYTKEILHEIGKMLNDVPNKISLSGHTDATPYPTGEK
                     SYSNWELSADRANASRRELIAGGMDANKVLRVVGLSSAVLFDKEDPFNPFNRRISIIV
                     MNEKAEKAITEEGPTTDIGNKDEVTKEKIEDSLIRP"
     gene            2441..3181
                     /locus_tag="PKZ49_05425"
     CDS             2441..3181
                     /locus_tag="PKZ49_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36037.1"
                     /translation="MQYPSLNALLIQIATWFISLAFLAFFSIEIELIELVFFQGVFAA
                     IASYFLRMAVWWFFIHLFFAPAIFIALTFNLPPLGYLMAFVLFILIYGSIHRTQVPLY
                     PSSKAVTNIITTLFPKNKAFSLIDLGSGGGKLLTSLSKLNPNGKFHGVEAAILPFLAS
                     RIRGFFSQSNCSFSWGNFWQYNLSSYDIVYAYLSPIPMDKLWKKVLQEMRPGSILISN
                     SFVVPDVPPSESIAINDFNNSVLYIWKI"
     gene            complement(3188..4573)
                     /gene="cysS"
                     /locus_tag="PKZ49_05430"
     CDS             complement(3188..4573)
                     /gene="cysS"
                     /locus_tag="PKZ49_05430"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HND36038.1"
                     /translation="MLKIYNSLTRAKEEFIPLVPGEVKLYVCGMTVYDFCHLGHGRVL
                     VVFDMVVRWLQSIGYNVTYVRNITDIDDKIIKRAQEMNESIETLTNRFIKAMHEDANA
                     LGTVKPNFEPCATHHVGEMITLIRKLIEKDCAYVADNGDVFFSVHHFPGYGKLSGKSL
                     SDLRAGERVEIDHHKRDPLDFVLWKSVKPGEPYWQSPWGNGRPGWHIECSSMSGHFLG
                     NHFDIHGGGQDLQFPHHENEIAQSEGANEETYVNYWMHNGFVRVDNEKMSKSLGNFFT
                     VREILKNFQAEVIRFFILRAHYRSPLNYSLEHLISAKNALDRLYTALKGYETVNAGKI
                     NWDNHHAQSFKIAMNDDFNTPEAIAILFNLSSEINRSQKPQDASLLKSLANILGLLQQ
                     EPQRYLQQDLVANNKNEKFSESEINLLINQRLEAKKNQDFKEADQIRSLLAEAGIILE
                     DSSQGTTWRRQ"
     gene            4724..5299
                     /locus_tag="PKZ49_05435"
     CDS             4724..5299
                     /locus_tag="PKZ49_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HND36039.1"
                     /translation="MIRLITLLLALSINLSALAAQPIVRIDTNLGPISLALYSDQAPK
                     TVENFLHYVREGFYAETIFHRVIPNFMIQGGGFDQALVQKPTYQPIINEAANGLKNEI
                     GTIAMARTSDPHSATAQFFINVANNTFLNYSSPTAQGYGYTVFGKVIEGMDVVNKIAA
                     LPTGPRGIFAKDVPKDTVVIKAIELLSDNKE"
     gene            5329..5820
                     /locus_tag="PKZ49_05440"
     CDS             5329..5820
                     /locus_tag="PKZ49_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HND36040.1"
                     /translation="MVKFHTNYGVITLALYDEQAPLTVKNFLSYVSSGHYDNTVFHRV
                     IDNFMIQGGGFDSNMMQKKTLEPIQNEASNGLKNETYTIAMARTSDVHSATSQFFINV
                     KNNSFLDFTSPTTQGYGYCVFGKVIEGMEVVDEIKKVKTGNYAGHENVPLENVVIERA
                     EIV"
     gene            complement(5834..6763)
                     /locus_tag="PKZ49_05445"
     CDS             complement(5834..6763)
                     /locus_tag="PKZ49_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006996 - organelle organization [Evidence
                     IEA];
                     GO_process: GO:0016567 - protein ubiquitination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIDE domain-containing protein"
                     /protein_id="HND36041.1"
                     /translation="MVTFSSSLTHEEYEFILMAVILIALISFYSFFKNWKRLRFIEDT
                     PTANLRSAHQGYVEIEGKGALIEDKPIYAPLSNHRCLWYRSQIECQEILIEKGQSRIN
                     WNVIYSNISHHSFKLTDGSTSCFVDPDSAEIQGEEKLVWYGNTEWPNQTQILESQSVL
                     HSNNNRYRYSESLILPGQSLYVLGQFSTSSAASQHSIREIMNNLILDWKKDSQHLLKR
                     FDANKDGQIDQQEWESARQIASAESQQIYQQRLLEPERNIITKPKDKRYPFIISVYSQ
                     ASLRQKYRLTAYLSLLACLVLTGYIIWFAHTHG"
     gene            complement(6781..7323)
                     /locus_tag="PKZ49_05450"
     CDS             complement(6781..7323)
                     /locus_tag="PKZ49_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HND36042.1"
                     /translation="MLVIAVIYGIMIYNSLVDVKHSVSQAWSNIDILLKQRHDELPKL
                     VETCKQYMGFEQETLKQVIAARSQVASARDAGNLPALGAAENMLRTGLGNLFAVVEDY
                     PELKSSEKFQHLQSRITGLENSIADRREYYNEAVKINNVRIEQFPEVIIANWFKFNAR
                     DLLQFNATEKQDVDVGKLFS"
     gene            complement(7443..8114)
                     /gene="trmB"
                     /locus_tag="PKZ49_05455"
     CDS             complement(7443..8114)
                     /gene="trmB"
                     /locus_tag="PKZ49_05455"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HND36043.1"
                     /translation="MSSAISIRSYAIRNGRYTNGQRKAYETLFSDFSIPFQQEALNLN
                     TIFGRCAPKILEIGSGMGETTATIAQQSPDNDYLAIEVHTPGIGSLLKLIDLQGLSNL
                     RIIQYDAVAVLNLMIEKESLDGVHIFFPDPWPKKRHHKRRLIQATFVTLLCERIKSGG
                     YLHIATDWQDYAEQILMVLSNQTSLINNNKDYAQRPTSRPLTKFEQRGIKLGHQVWDL
                     IFTKK"
     gene            complement(8145..8939)
                     /locus_tag="PKZ49_05460"
     CDS             complement(8145..8939)
                     /locus_tag="PKZ49_05460"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HND36044.1"
                     /translation="MDDFIIAGKCYKSRLLVGTGKYKDFSETRAAIEASGSEIITVAI
                     RRTNIGQNPDEPNLLDVLPPSKFTLLPNTAGCYTAEEAIRTLRLARELLDGHSLVKLE
                     VLGDSKTLFPNIVETIKAAQTLVKENFQVMVYTSDDPIVAKQLEEVGCIAIMPLASLI
                     GSGMGILNPWNLQIMIDRATVPVLVDAGVGTASDATIAMELGCDGVLMNTAIAAAKDP
                     VMMASAMRKAVEAGREAYLAGRMEKKFYSASPSSPIDGISPLSSHK"
     gene            complement(9006..9209)
                     /gene="thiS"
                     /locus_tag="PKZ49_05465"
     CDS             complement(9006..9209)
                     /gene="thiS"
                     /locus_tag="PKZ49_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HND36045.1"
                     /translation="MIQLIINGEPQQFESSLNLQQLLENMGLQNKRIAIEHNGEIIPR
                     SRFSDYIVTEGDRLEIVVAVGGG"
     gene            9269..9342
                     /locus_tag="PKZ49_05470"
     tRNA            9269..9342
                     /locus_tag="PKZ49_05470"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9301..9303,aa:Gly,seq:ccc)
     gene            9418..10011
                     /locus_tag="PKZ49_05475"
     CDS             9418..10011
                     /locus_tag="PKZ49_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc protease"
                     /protein_id="HND36046.1"
                     /translation="MKRKIRGLFVFFWIVTLLIISHAVLADNKISQPESKLIVGWVEF
                     IRLEPWGMKMRARIDTGANTSSMSARDIHQFKKGNEDWVKFVLDFKTDKGKPNRSVEI
                     ERPITRIVKIKQHQGTLQERPVVNMDICIANQVHNIEFNLIDRRGLNYPVLLGRRALE
                     NLALVDPARTFIEKADCGHIKKKKKKADTPPDSISLE"
     gene            10008..11534
                     /locus_tag="PKZ49_05480"
     CDS             10008..11534
                     /locus_tag="PKZ49_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inactive transglutaminase family protein"
                     /protein_id="HND36047.1"
                     /translation="MRQLHLLFLTLLLCAIGLGLAAYKVDTLGLPLTASAQTEVWNVE
                     ARISFKAKPDSGVKVSLPLPFNPSGYTVLNEDFVGANYGLATEQDEGGRVALWAKRRA
                     QGKQLLFYRAVIFENEEKIADSNKIPSYPAVPDYPEHFLPVIQGLLDKSRQQSADTLS
                     FAQQVIQQLNATAPAPELKLLIEHINTSKNLAKGISWILAGARVPARVIQGLRLHDGK
                     RYSELEAWVQVHDGKQWRTLNTKDGQEGLPTNFVIWQIGDQKTFSVEGGTEISLQFSV
                     AKALRELVDVAESRAILKGSRIMEFSLYSLPVQTQNVYKVLLMIPLGAFVVVLMRNLI
                     GVKTFGTFMPVLIALAFRETELVWGLALFCLIVGLGLLLRAYVEQLKLLLIPRLASVL
                     TMVVLLMAGTSVVMHKLGLEMGLSVALFPMVIMTMTIERMSLTWEEAGPIEAFKQGAG
                     SLLVATIGYLVMNIDELQHLLFVFPELLLVVLAVTILFGRYSGYRLTELWRFRALAKR
                     "
     gene            11544..12491
                     /locus_tag="PKZ49_05485"
     CDS             11544..12491
                     /locus_tag="PKZ49_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-glutamate ligase-like protein"
                     /protein_id="HND36048.1"
                     /translation="MFSTWLNLKRAGVIGMNRRNADFILRYNERSLYPLVDDKLRTKQ
                     LAIAAGIAVPQLYGVIEIEHDIVNLPGIVKDYKDFVIKPAHGSGGNGIAVIIGRINER
                     FQYNNGSLLTEEELEYHISNILSGMYSLGGQPDQALIEYRVKFDPLFESVSYRGVPDI
                     RTIVFRGVPVASMVRLPTRLSDGKANLHQGAVGAGIDLTSGVTTRAVWQNRPIDIHPD
                     TGSAISGLAIPHWNKLLTLAAQCYELVGLGYLGVDVVLDHELGPLVLELNARPGLSIQ
                     IANGKGMEKRLEYIDRLSEIPEKAQDRAALAQSLFKAEN"
     gene            12555..13577
                     /gene="glk"
                     /locus_tag="PKZ49_05490"
     CDS             12555..13577
                     /gene="glk"
                     /locus_tag="PKZ49_05490"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HND36049.1"
                     /translation="MDSRFIIYGDIGGTKTILRLAKQQNSDLLHIREQRYASQDYQNL
                     DEIINDFLIGLPDTNAANKAISAACFAIAGPIAAQQARLTNLPWLISAADLSRKFAIP
                     QVKLINDFEATALGVEKLTQQDLEVIQIGNMNEASMRVVLGAGTGMGVAWIASIGNRY
                     IPFPTEAGHTDFAPINDLQIELLNFLAAKFGHVSIERVLSGPGLTNIFNFFQQRANHL
                     RSSSLPELSEDEGQLIIQLALEQNHVTAIESLQLFTEIYGAFAGNLALTGLCRGGVYI
                     AGGIAPRILHYLKTERFIQAFRNKGRFTGLMREIPLSVILNTQVGLLGAESEAQRLLQ
                     NYLLIL"
BASE COUNT         4095 a         2830 c         2836 g         4071 t
ORIGIN      
        1 ttcttgtata caaaacctcc gccacggtta agttaatttt gcaggaccga ctaaatacct
       61 tgggatattc aattagccgt tggaagacaa ggaaattaat gcacgaggtt ggcgtgactg
      121 tcaggtatcg aaagaaatac aaagtggtga cggacagtga tcatccgttg ccggtatttg
      181 aaaaccgatt aaaccggcaa tttgatgttg ccagccggat caaatttatg tttacgatat
      241 cacctgcatc tggacacaag aaggctagct gtatccggca atcgtaatta tcgttgtgtt
      301 ctataacaac caaacggcat tcatacctga attacaagag cctaaatcaa tatgaagcag
      361 aagcagtaaa atcgataaaa gcagcttaac tgatgtgtcc ggttttactt gaccaggtca
      421 caactccact ggtgacaaag aaggcagaat cgctcgagct ttattttatg agaggcaatt
      481 accaattggt ctttagcgcc ctctgtttga gtcttgctgc taaagtagca ggcacattag
      541 ccgttacaat tgaaaatttc caggattctt caaatgttag tatcggttgg ttatcttatt
      601 gtaatagtat ctgtttttgg cggctttgcc ctcgctggag gacatttagt atcattattt
      661 caaccagttg aactgttgat gattggggga ggggctcttg gagcgttttt tgttggaaat
      721 tcaggtaaag ctattaaggc aaccctgaaa gcactacctc atgtttttaa aggtgccaaa
      781 tatactaaag atgtatatat ggagctgatg actctgcttt atgagatatt gggaaaaata
      841 cgcaaagaag gactaatggc tatagaagcc gatgttgaag atcctcacaa aagtccatta
      901 ttctctaaat atccaaaaat tttatcagat caccatgtta ttgaatttat tacagattat
      961 ttgcgcttga tggttggtgg aaatctaaat ccattagaaa ttgaaaatct tatggatggg
     1021 gaaattgaaa cgcatcacca agaggaagaa gttcctattc atgcaattgc aaaactaggt
     1081 gatgggcttc cagcatttgg aattgtcgcg gccgtcatgg gcgttgtaca caccatggag
     1141 tctgttggaa ttcccccggc agagctaggg aagctcattg cagccgctct ggtcgggacc
     1201 tttctaggaa ttttacttgc atatggattt gttggtcctc ttgctagcaa attagaacaa
     1261 cgccttcatg aatcaagtaa gttgctagaa tgcattaaag ttacgctatt agctaatctg
     1321 aatggttacg cgccagctct tgcaattgaa tttggaagaa aggttttata ttcgacagaa
     1381 agaccttcgt ttgcagagct agagaaacat gtgaagcaaa gcaaggctaa gtgatagcga
     1441 atcccctata aaacttcaaa gagatgactg atgtctgacg atctttcaca aaagcctata
     1501 attatcaagc gcatcaagaa agtaagtggg gggcatcacg gtggtgcatg gaagatagca
     1561 tatgcagatt ttgttacagc aatgatggct ttctttctat taatgtggct actaggctcg
     1621 accactcaag gcgataaaga aggtatcgca gagtatttta aaacaccatt aaagatagca
     1681 ctagcgggag gggctgggag tggtgatagt tcaagtatta taaagggtgg gggggaagat
     1741 ctaagtcgcc ggatgggtca ggtaaaaagg ggcgatattg aagaaattaa gagaactatc
     1801 aatttagagg cagcgcaagc tgaaaaacag cggcttgaaa tagaacaatt taaaaatctc
     1861 aaaaaaagga ttgaacaggt aattgaggca aatcccagtt taataaattt taaaaagcaa
     1921 ttattgcttg atatcacaac cgatggactc cgtatacaaa ttattgatga aaaaaaccga
     1981 ccaatgtttt caattggtaa ggccgaactg caatcctaca caaaagagat cctgcatgaa
     2041 atcggcaaaa tgctaaacga tgtaccaaac aaaataagtc tatcagggca tacggatgcg
     2101 acaccctatc ccactggtga aaaaagctat agcaattggg aattatctgc agaccgagca
     2161 aatgcatccc gtcgagagtt aattgcggga ggtatggacg caaataaagt tctccgtgtt
     2221 gtgggactgt cttcagctgt tctatttgat aaagaagatc catttaaccc attcaatcgt
     2281 cgtatcagta ttatcgttat gaatgaaaaa gctgagaaag ctattacaga ggaaggacca
     2341 acgactgata ttggaaataa ggatgaggtc actaaagaaa agattgagga ttcacttatt
     2401 cgaccttagc gatctacttc taacccttat atttaaaaag atgcagtatc cttcactaaa
     2461 tgcgttactt attcaaatag ctacgtggtt tatatcatta gcttttctag cttttttctc
     2521 gatagaaatc gagctaattg agctagtgtt ttttcaaggt gtatttgctg cgattgctag
     2581 ctatttcttg agaatggctg tttggtggtt ttttattcat ttgttttttg cccctgcgat
     2641 atttatagca ttaactttca acttaccccc tctgggttac ttgatggctt ttgtgctatt
     2701 tatcttaatc tatggaagca ttcatcgtac ccaagttcct ctttacccct ctagcaaggc
     2761 tgtcacaaat ataataacaa cgctatttcc aaaaaataaa gcattctcac ttatagatct
     2821 gggttctggg ggagggaaat tacttacttc cttgagtaag ctaaacccaa atggaaaatt
     2881 tcatggcgtt gaggccgcaa ttttaccttt tctagcaagt aggatcagag gatttttttc
     2941 tcaaagcaat tgcagctttt cctgggggaa tttctggcaa tataatctct catcctatga
     3001 catagtttat gcatatttat cgcctattcc aatggataaa ctttggaaaa aagtcctaca
     3061 ggaaatgcgg ccaggaagta ttttgataag taattcgttt gtagtacctg atgttcctcc
     3121 ctcagaatct attgcaatca atgatttcaa taattctgtt ctttatatct ggaagatatg
     3181 attctattta ttgacgtctc catgtcgttc cttgtgaact atcctcaagt atgattccag
     3241 cttcagcaag caagctccgt atctgatctg cttccttaaa atcctgattt ttttttgcct
     3301 ctaggcgctg attaatcagt aaatttattt cactttcaga aaatttttca tttttattgt
     3361 ttgctactaa atcttgctga aggtatctct gtggctcttg ctgcaataag cccagtatat
     3421 ttgccagtga cttgagtaaa ctcgcatcct gcggcttttg agatctattt atttcactgg
     3481 aaaggttgaa taagatggca attgcctctg gagtattaaa atcatcattc atagctattt
     3541 taaaactttg tgcatgatga ttatcccaat taatcttgcc agcattcact gtttcatagc
     3601 cttttaatgc tgtataaagg cgatcaaggg catttttagc actaatcaga tgttcaaggg
     3661 aataatttaa agggcttcga tagtgcgcac gtaaaataaa aaaacgaatc acttcagctt
     3721 gaaaattttt taaaatttct cgtactgtaa aaaaatttcc taaagatttg gacatctttt
     3781 cattatctac tcggacaaat ccattatgca tccagtagtt tacataagtc tcttcatttg
     3841 ctccttcact ttgagcaatt tcattttcat gatgtggaaa ttgtaagtct tgaccaccac
     3901 cgtgaatatc gaaatgatta cctaagaaat gtccactcat cgatgagcac tcaatatgcc
     3961 agccaggcct cccatttccc cacggagatt gccaataagg ttctccaggt ttcactgatt
     4021 tccataaaac aaaatcaagg ggatcacgct tgtgatgatc aatctctact ctttcacccg
     4081 cacgtagatc agacaatgat ttccccgaaa gctttccata cccaggaaaa tgatgtacag
     4141 aaaaaaatac atctccattg tcagctacgt aagcacaatc tttctcgatt aacttacgga
     4201 taagggtaat catctctccc acgtgatgag ttgcgcatgg ctcaaaattt ggctttacgg
     4261 ttccaagggc attggcatct tcgtgcatag ctttgatgaa acgattagtt aatgtctcta
     4321 ttgactcatt catttcttgc gcacgtttaa ttattttatc gtcaatatcc gtaatattac
     4381 gcacgtaggt aacgttatac ccgattgatt gtaaccaacg aacaaccata tcaaatacaa
     4441 ccaatacgcg tccatgacca agatgacaga agtcatatac cgtcatacca caaacataaa
     4501 gttttacttc acccggtact aatggaataa actcttcctt tgctcgagtt aatgagttat
     4561 aaatttttag catcgtaact acaattaaat tgtgagataa ataaacttta ttgatagaat
     4621 caataaataa gataaagttg aatcaatcct attaatctag gattctagca gtagcaccaa
     4681 ctggtgtata ttttctcctt tcattattac tgattgaatc attatgataa gactgattac
     4741 gctattactc gcattatcga ttaacctttc agcgttagct gctcaaccaa ttgttaggat
     4801 tgatacaaat ttaggaccga tctcgttagc gctttattct gaccaagcac ctaagacggt
     4861 agaaaatttt ttgcattatg ttcgtgaagg tttctatgcg gagaccattt ttcatcgcgt
     4921 tattcccaac ttcatgatcc aaggcggggg atttgatcaa gcattagttc aaaagccgac
     4981 ataccaacca atcataaatg aagctgcgaa tggcttaaag aacgagatcg ggacaattgc
     5041 aatggctaga acgagtgatc ctcattcggc tacagctcag ttttttatta atgtagcgaa
     5101 taatacattt cttaattatt cctccccgac agctcaaggc tatggttata ctgtttttgg
     5161 caaggttatc gagggaatgg atgttgtaaa caaaattgca gcgctcccaa ctggacccag
     5221 gggtattttt gctaaagacg tgcctaagga cacggtagta attaaagcaa ttgagcttct
     5281 ttctgataac aaagaataaa aaatcgattc acattatagg tagatcaaat ggttaaattt
     5341 cacactaatt atggtgtaat tacactcgct ttatacgatg aacaagcacc gcttacagta
     5401 aaaaactttc taagttatgt aagtagtggg cactatgaca atacagtgtt tcaccgcgta
     5461 attgataatt ttatgattca aggtggcgga tttgattcca atatgatgca aaagaaaacc
     5521 ctagagccca ttcagaatga agcaagcaac ggtttgaaaa atgagaccta tacgattgcg
     5581 atggctcgaa cttcagacgt tcattccgca acctcacagt tttttattaa tgttaaaaat
     5641 aatagctttc tcgattttac ttcacccaca acccaaggat atgggtactg tgtttttgga
     5701 aaagtaattg aaggaatgga agttgtggat gagataaaaa aagtaaaaac gggtaactat
     5761 gccgggcatg agaatgtacc gctggaaaac gtagtcatag aaagagccga gattgtctag
     5821 ttcgaatatt taattatcca tgggtatgcg cgaaccatat gatatagccg gttaatacta
     5881 agcaggcgag aagcgataag taagctgtaa gccgatattt ttgcctcaac gaagcttgcg
     5941 aataaacaga aataataaat ggataacgct tgtccttcgg tttagtaata atattgcgct
     6001 caggctctag taatcgttgc tggtatattt gttgtgattc tgcactcgct atttgacgtg
     6061 cagattccca ttcttgctgg tcgatttggc catctttatt cgcatcaaaa cgttttagta
     6121 aatgctgaga atcttttttc caatccagta ttagattatt cataatttca cggatactat
     6181 gctgtgaagc agccgagctt gtactaaatt gacctaacac ataaagcgat tgtcctggca
     6241 aaatcagtga ttcactataa cgatatcgat tgttattgct atgcaagact gattgacttt
     6301 caagtatctg tgtttgattg ggccattcgg tgttgccata ccatactagc ttctcctcac
     6361 cctggatttc agcactatca ggatcgacaa agcagctagt tgacccatct gtcaatttaa
     6421 atgaatgatg gctaatattg ctatagatca cattccagtt gattcgtgat tgtcctttct
     6481 caataagaat ttcctgacac tcaatctgac tccggtacca taagcagcga tgatttgaaa
     6541 gtggcgcata aataggttta tcctctatca acgctccttt cccttctatt tcaacataac
     6601 cctgatgggc agaacgtaaa tttgcggttg gagtatcttc aataaaccga agtcgcttcc
     6661 aatttttgaa gaaagaatag aagcttatca gagcgatcag aatgaccgcc attaaaataa
     6721 attcatattc ctcatgcgtc aaagaagatg aaaaagtcac cacagcgagt aagcgatttg
     6781 ctagctaaaa agtttaccta catcaacatc ttgtttttca gtagcattga attgaagtaa
     6841 atctcgcgca ttaaacttaa accaattcgc aataataacc tctggaaatt gctcaatacg
     6901 aacgttattg attttaacgg cttcattgta gtattcgcgg cgatccgcga tgctattttc
     6961 taggcctgta attcgcgatt ggagatgttg aaatttctca ctggatttga gttctggata
     7021 atcttcgaca acagcaaaca agttaccaag acctgttctt aacatatttt ccgcagcacc
     7081 taatgctggt aagttaccgg catcacgcgc gctagcgacc tgagatcgtg cagcaatgac
     7141 ttgcttaagg gtttcttgct caaaacccat atattgctta caggtctcaa caagcttggg
     7201 caactcatca tggcgttgct ttagaagaat atcgatatta gaccacgcct gcgatacgct
     7261 atgcttgaca tcaaccagac tgttataaat catgatcccg taaataaccg caattaccag
     7321 cattgcaatc aaaagataga ttatcgtcgt agtatccaat gttaaacctc cgctatgtta
     7381 ggttttgtag aatacctgag tcatgcattg ttagataaga aaaaaagaaa cttgtccccg
     7441 ccctatttct tcgtaaaaat taaatcccac acctggtggc ctaatttaat tccacgttgt
     7501 tcaaacttag ttaaggggcg acttgtcggg cgttgagcat aatctttatt attattgatt
     7561 aaactcgttt gattacttaa aaccataaga atctgctctg cgtaatcttg ccagtcagtt
     7621 gcaatatgta aatagccccc tgatttaatg cgttcacaaa gcaatgttac aaacgttgcc
     7681 tgaataagtc gtcgtttatg atgtcttttt ttgggccatg gatcagggaa aaaaatatga
     7741 acgccatcaa gggattcttt ctcaatcata aggtttaata cagctaccgc atcatattga
     7801 atgatgcgca aattacttaa accctggaga tcaattaatt ttaataagct gccgataccc
     7861 ggtgtatgaa cttcgatagc caaataatca ttatcaggtg attgttgagc aatcgtggcc
     7921 gttgtttcgc ccatacctga gcctatttca agtatcttgg gtgcacagcg cccaaaaatg
     7981 gtgtttaggt taagtgcttc ttgctggaaa gggatgctga aatccgaaaa taatgtttca
     8041 tatgctttgc gctgcccatt tgtgtatcgc ccattacgaa tcgcatagct acggatagat
     8101 atagcagatg acattttaaa cctgaataat caacaccaag tagattactt atgcgatgac
     8161 agcggtgaaa taccatcgat aggagagctt gggcttgctg agtaaaattt tttctccatc
     8221 cgaccggcaa gataagcttc tctccccgct tcaaccgcct tgcgcatcgc cgaagccatc
     8281 atgacgggat cttttgccgc tgctatcgcg gtattcatta acacgccatc acagccaagt
     8341 tccatggcaa tagtagcatc agaagctgtt cctactccag catctaccaa aactggcaca
     8401 gttgcgcgat cgatcatgat ttgtaagttc catggattta aaatgcccat gcccgatcca
     8461 attaatgaag caagcggcat aatcgcaatg caaccaactt cttcaagctg ttttgcaaca
     8521 atcggatcat ctgacgtata aaccatgact tgaaaatttt cttttacaag cgtctgcgca
     8581 gctttgatgg tctctacaat attgggaaac agtgttttag aatctcccag tacttcgagt
     8641 ttcaccaagg aatgtccatc cagtaattct cgtgctaacc gcaacgttcg aatcgcttct
     8701 tcagcagtat aacaaccggc tgtattgggc aataacgtaa attttgatgg gggaagaaca
     8761 tccagtagat ttggctcatc tggattctgt ccaatattag tacggcggat tgcaaccgta
     8821 atgatctctg aaccacttgc ttctatggct gctcgcgttt cagaaaaatc tttatatttg
     8881 cccgtaccaa ctagcagccg ggatttatag catttacctg caattataaa atcatccata
     8941 aataactaag cttttgaagt catgatttgc agcaaatgag caagtccagt aataagatta
     9001 cacaactaac caccaccaac tgccaccaca atctcgagtc gatcaccctc tgtcacgata
     9061 tagtccgaaa atctacttct tggaataatt tcaccattat gctcgattgc aatgcgctta
     9121 ttctgaagcc ccatattctc aagcaattgt tgcaaattga gcgaggattc aaattgttgc
     9181 ggctcaccgt tgataatcag ttgtatcatt tgaaatataa caagtgtagg gtaaactttg
     9241 tggttttcta attaaatgat tttagcacgc gggtgtagct caatggtaga gcagaagctt
     9301 cccaagctta agacgagggt tcgattccct tcacccgctc catagcagcg accgattgga
     9361 aaagttgggt gcagtaaata cgttataatg taactactta aaatcttgga tcttttcatg
     9421 aaacgtaaga tcagggggct atttgttttt ttctggatag taaccctatt aataataagt
     9481 catgctgtat tagctgataa taaaatttct caacccgaat ctaaacttat cgtcggttgg
     9541 gtagaattta ttcgccttga accttggggt atgaagatgc gtgcccgtat tgacactggc
     9601 gccaatacat cttcgatgag cgcacgtgat attcatcaat tcaagaaagg aaacgaggac
     9661 tgggtcaaat ttgtactgga ttttaaaacg gacaaaggta aacccaatcg ctcagttgag
     9721 atcgaacgac ctatcacccg catcgtaaaa atcaaacaac atcaaggaac actacaagaa
     9781 cgccctgtcg tcaatatgga tatttgtatc gccaaccaag tccataatat tgagtttaac
     9841 ttgattgatc ggcgtgggct gaattatccg gttctgctgg gacgccgtgc cctagaaaat
     9901 ttagcattag ttgaccctgc tcggactttt attgaaaaag ctgattgtgg tcacatcaag
     9961 aaaaaaaaga aaaaagcaga tacaccacca gattcaatca gtcttgaatg agacaactac
    10021 atttattgtt cttaacgtta ctgctatgcg ctattggatt agggctggca gcctataaag
    10081 tcgatacact gggtttacca ctgacagctt ctgcacaaac cgaggtttgg aatgtcgaag
    10141 cacgcatcag cttcaaagcc aaacccgata gtggagttaa agtcagctta ccgctccctt
    10201 ttaatccttc aggctacaca gtcctcaatg aagattttgt gggcgctaat tatggactag
    10261 caaccgagca agatgaaggt gggcgagttg cattatgggc aaaaaggcgt gctcaaggta
    10321 aacagttatt attttaccgc gcagttatat ttgaaaatga agagaaaata gctgactcca
    10381 ataaaattcc aagttatcct gctgttccag attatcccga acatttctta cccgtcattc
    10441 agggtttatt agataaatcc cgtcagcaat ctgcggatac cctttcgttt gcacaacaag
    10501 tcattcagca actcaatgcc acagcacctg cgccggaatt aaagttactc attgagcaca
    10561 ttaatacgtc caaaaatcta gcgaaaggga ttagctggat tctagccggt gcaagagttc
    10621 cagctcgggt tatccaaggg ttacgtttac acgatggtaa gcgttatagc gaactcgaag
    10681 cttgggtgca agttcatgat ggtaagcaat ggcgaacact taataccaaa gatgggcaag
    10741 aaggcctacc taccaatttt gttatctggc agatcggtga tcagaaaacg ttttcggttg
    10801 aaggcggaac tgaaattagt ttgcaatttt ctgttgcaaa agcactccgt gagctcgttg
    10861 atgtcgccga gtcccgcgct atcttgaaag gctctcgcat catggagttc tcactgtatt
    10921 ctttaccagt gcaaacccag aatgtttata aagtcttgtt aatgattcca ttaggcgcat
    10981 ttgtcgtggt tttgatgcgt aatctcattg gagttaaaac attcggtaca ttcatgcccg
    11041 ttctgatcgc acttgcgttt agggaaactg aactcgtctg gggacttgcc ttgttttgtt
    11101 tgatcgtggg tctagggtta ttattgcgcg cttatgttga gcaactcaag ttattgctca
    11161 tccctcggct tgcttcggta ttgaccatgg tggtgctgct catggcaggg accagcgtag
    11221 taatgcataa gcttggacta gaaatgggcc tatcagtagc acttttccct atggtaatca
    11281 tgaccatgac cattgaacgc atgtcgctga cttgggaaga agcaggacca atcgaagcgt
    11341 ttaagcaggg cgccggcagt ttgttggttg cgacaattgg ttatttggtg atgaatatcg
    11401 atgaattgca gcatttatta tttgttttcc cagaactatt actcgtcgtg ttggcggtaa
    11461 ctatcttatt tgggcgctac tccggttatc ggttaacgga actgtggcga ttccgcgctt
    11521 tagcaaaacg gtagctaaat gcaatgtttt cgacctggct aaatctcaag cgtgctgggg
    11581 taatcggtat gaatcggcgt aatgccgatt ttatcctacg ctacaacgaa cgttcacttt
    11641 atcctttagt cgatgacaaa ttacgcacca agcaactagc gattgctgct ggaattgcag
    11701 ttcctcagct atacggagtt atcgaaatcg agcacgatat cgttaacctt cctggcattg
    11761 ttaaggatta caaggatttt gtcattaaac ctgcccatgg ttccggtggt aatggtatcg
    11821 ctgtcatcat tggtcgcatc aatgagcgat ttcaatacaa taatgggagt ctcttgactg
    11881 aagaagagct cgaatatcac atttccaata tcctaagcgg tatgtatagt ctgggaggac
    11941 agcctgatca agcattaatc gaatatcgcg tgaaattcga ccccttattt gaatcggtca
    12001 gttaccgcgg tgttcctgac attcgcacca ttgttttcag gggtgtgccg gttgcttcaa
    12061 tggtacgttt accgacacgg ttgtccgatg ggaaagctaa tttacaccaa ggcgctgtgg
    12121 gcgcaggcat tgaccttacc tcgggtgtta ctactcgcgc agtttggcaa aatcggccga
    12181 tcgatatcca cccggatact ggatcagcta tcagcgggct cgccattccc cactggaata
    12241 aattactaac actcgccgca cagtgttatg agttagtcgg attaggatac ctcggcgtcg
    12301 acgtggtatt ggatcatgaa ctgggtccat tggtattgga gctcaatgca cgcccgggct
    12361 tgtccattca gattgccaat ggaaaaggaa tggagaagcg attggaatat attgatcggc
    12421 ttagtgaaat tccagagaaa gcacaagatc gtgctgcact tgctcagtca ctgtttaaag
    12481 cagaaaacta aagccagggc agcaatcact gaatagcagg attaattaca ataaacgcct
    12541 agccaggcca tcctatggac tctcgattta ttatttacgg cgatatcggc ggcacaaaaa
    12601 ccattctgcg gttggcaaaa cagcaaaata gtgatttatt gcacatacgg gaacagcgct
    12661 atgcgagcca ggattatcaa aatctagatg aaatcatcaa cgatttttta atcggattac
    12721 cagatacgaa tgcagcaaac aaggccatct cagccgcctg ttttgcaatt gcaggcccga
    12781 ttgctgcgca acaagcacga ttaactaatc taccatggct aattagcgca gcagatcttt
    12841 caagaaaatt tgctattccc caagttaagt tgatcaatga ttttgaagca acagcactgg
    12901 gggttgagaa attaacacag caagatctcg aagttatcca aataggaaat atgaatgaag
    12961 ctagtatgcg cgttgtgctc ggtgcgggca ctggtatggg agttgcctgg atagcaagta
    13021 ttggaaaccg ctatattccc tttccaactg aggcagggca taccgatttt gcgccaataa
    13081 acgatttgca aatcgaatta ctcaacttct tagctgcaaa atttggccat gtttccatcg
    13141 agcgagttct ctctggaccc gggttaacca atatttttaa tttttttcaa caacgcgcga
    13201 accatctgcg gtcctcctcc ttgccagaat tgagtgaaga tgagggtcaa ttgattattc
    13261 aattggcgct agagcaaaac catgtcacag caatcgagtc cctacagcta ttcactgaaa
    13321 tctatggtgc atttgctgga aatttagctt taacaggact atgtcgtggt ggggtttaca
    13381 ttgctggggg cattgcacca cgaattctgc attacctaaa gacggaacgc tttatccaag
    13441 cattccgcaa caaaggtcgt ttcacaggac taatgcgtga aataccgctc tctgtcatcc
    13501 taaataccca ggttgggctc cttggtgcgg aaagtgaagc gcagcgattg ctacaaaatt
    13561 atcttctaat actttgaaaa ttacctaaac tcattcagtt agaatgcgtt ctctcacccc
    13621 ggatgaacta caatacttaa tcaatgctgc aagcagtgca gctagttgta gctccctctc
    13681 tcaccccgga tgaactacaa tatatctggc aactgcaaaa cgaccctgaa agttgtagct
    13741 ccctctctca ccccggatga actacaatca gacgagcacg ggaaaataca acattcatgt
    13801 tgtagctccc tctctcaccc cggatgaact ac
//
LOCUS       DAOQKQ010000060        13756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_2094_length_13756_cov_5.821911, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000060 DAOQKQ010000000
VERSION     DAOQKQ010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 13756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13756
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2094_length_13756_cov_5.821911"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..454
                     /gene="rpsR"
                     /locus_tag="PKZ49_05495"
     CDS             173..454
                     /gene="rpsR"
                     /locus_tag="PKZ49_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HND36050.1"
                     /translation="MARFFRNKDNNLKAKEKLANRPLFKRKKFCRFTAEGIKTIDYKD
                     IDILKDFINENGKIIPARITGTKTFYQRQLNTAIERARFLSLLPYTDQH"
     gene            475..930
                     /gene="rplI"
                     /locus_tag="PKZ49_05500"
     CDS             475..930
                     /gene="rplI"
                     /locus_tag="PKZ49_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HND36051.1"
                     /translation="MQIILLEKIGNLGQLGDIVNVKKGYARNYLIPQGKAKWATESAI
                     AEFNTKRAELEKKQAEKLGSAQALAAKLNGLLLQVTQKSGVDGKLFGSVTSSNILESL
                     EAQGFSVEKSMIRMPEGQIKQIGDYPITIAIHHDVLAQITVSVLGETTA"
     gene            1051..2445
                     /gene="dnaB"
                     /locus_tag="PKZ49_05505"
     CDS             1051..2445
                     /gene="dnaB"
                     /locus_tag="PKZ49_05505"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HND36052.1"
                     /translation="MAQSSIPISITHNELLDIYKTPPHSIEAEQSVLGGLMLDNQAWD
                     KIADIITIADFYRHDHQLIYHHICNLIEQNKPADVITVAESLDNSAELQNIGGLAYIG
                     SIVQSTPTAANIRRYAEIVRERAIMRKLAEVSTQIAESAYNPTGRSATDLLDNAESKI
                     FEIAEAGARGKQGFIDIQPLLKQVVERIELLYSKENPSDVTGIASGFHDLDQKTSGFQ
                     PGDLIIVAGRPSMGKTAFSLNIAEHVALELSKPVAIFSMEMGGAQLALRMLGSIGRLD
                     QHKVRTGRLDDDDWPRLTHALGKLNDAPIFIDESAALNALELRARARRLYRQHGELGL
                     IVVDYLQLMSSHSQNENRATEISEISRSLKGLAKELQVPLIALSQLNRGLEQRPNKRP
                     IMSDLRESGAIEQDADVILFIYRDEVYNPDTQDKGVAEIIIGKQRNGPIGKVDLTFLG
                     EYTRFENYAKPDYY"
     gene            complement(2471..2857)
                     /locus_tag="PKZ49_05510"
     CDS             complement(2471..2857)
                     /locus_tag="PKZ49_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HND36053.1"
                     /translation="MTRRLISSGSTFEEEIGYSRAVVEGDWIFVSGTTGFDYSTMSIS
                     EDLLEQAEQCFKNIELALKQANSSLKDVVRVTYVLPEASDFEKCWPIFRKYLGDVRPA
                     AMMISAGLSDPRMRIEIQVTARKCVL"
     gene            3203..3607
                     /gene="cheY"
                     /locus_tag="PKZ49_05515"
     CDS             3203..3607
                     /gene="cheY"
                     /locus_tag="PKZ49_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HND36054.1"
                     /translation="MSADKNLKFLVVDDFSTMRRIIRNLLRELGFLNVEEAEDGAAAL
                     RKLRESSFDFVVSDWNMPNMDGLTMLQSVRADEELKQIPVLMVTAEAKKENIIAAAQA
                     GANGYIVKPFTAATLDEKLNKILQNIAHKAAS"
     gene            3643..4326
                     /locus_tag="PKZ49_05520"
     CDS             3643..4326
                     /locus_tag="PKZ49_05520"
                     /inference="COORDINATES: protein motif:HMM:NF016247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HND36055.1"
                     /translation="MNNPTNSSPTEISADDKLLERIGKLTRLLHNSICELGYDKDKKL
                     QEASEQIADIHERLNYVLSKTQSAAETTLSAIENIKPIQSNLSSSAIILTQEWQELIK
                     LENSCHSNKNVAKFSNLFMRTVSYLENIPKQAEVTNDYLVTVIMAQDFQDLTGQVIKK
                     ISHLLLLIENELIQVLLDNSISTNPATINNKSSPNNLLNGPTINPQKKPEILSNQTDV
                     DNFLADLGY"
     gene            complement(4351..4938)
                     /gene="coaE"
                     /locus_tag="PKZ49_05525"
     CDS             complement(4351..4938)
                     /gene="coaE"
                     /locus_tag="PKZ49_05525"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646453.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HND36056.1"
                     /translation="MTGGIGSGKTSVAEIFQTLGITIIDTDEIAHELTHENGEAISHI
                     KEIFGGEYITDTGRLDRVKMRNLVFTDLSMKKKLEEILHPLIYRNVEQLIPTIESLYG
                     IVAIPLLFETGCFCNLIDRVLVVDCPEELQILRTVQRSKLSQKDVRAIIKTQISRKER
                     LARANNIISNDRDFIYLVDQVRKLHSEFTSFTKMK"
     gene            complement(4963..5823)
                     /locus_tag="PKZ49_05530"
     CDS             complement(4963..5823)
                     /locus_tag="PKZ49_05530"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HND36057.1"
                     /translation="MAFINSFQASPELFVTICGVLGITVGSFLNVVIYRLPKMMELAW
                     HQQCAELKGEPAKLLPVFNLITPRSNCPHCGHKITMLENIPLISYLFLRGRCSQCHEH
                     ISFCYPTVELLAGLMSGFAAWYYGYSFMCFAALIFIWAMIALTFIDFDTQLLPDAITL
                     PLLWIGLLINLNNGIIDLQSAVIGAVAGYLVLWVVYWIFKLITGKEGMGYGDFKLLAA
                     IGAWFGWQTLPLVILSSSFVGGLVGIFLIYFRNQHSGLAIPFGPYLAGGGLIAMFWGK
                     QINHSYLSLF"
     gene            complement(5859..7082)
                     /locus_tag="PKZ49_05535"
     CDS             complement(5859..7082)
                     /locus_tag="PKZ49_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HND36058.1"
                     /translation="MATVAASNDKKVKEISFTWEGKDKTGKLIKGEMRAAGVEVVNST
                     LRRQGIRVTKVAKTKSSGSISDKDITLFTRQLATMMKSGVPLLQAFDIVGKGHSNPAV
                     GKLLMDIKLDVETGSSLANAFRKYPLYFDSLFCNLVAAGEAAGILDSLLDRLATYKEK
                     IQAIKGKIKSALFYPISIIVVAFVITAVIMIFVIPAFKELFQGFGADLPGPTLMVMTI
                     SDFFVDYWWAIFGGIGASLYSFFYAWKRSIAMQRVMDKLLLRLPIFGDVIRKATVARW
                     SRTLSTMFAAGVPLVEALDSVAGAAGNYVYYEATKHIQSEVSTGSSLVAAMTETQVFS
                     SMVLQMVAIGEEAGSLDAMLSKVADFFEAEVDNAVEALSSLMEPMIMVVLGTLIGGMV
                     VAMYLPIFKMGQVVG"
     gene            7355..8617
                     /locus_tag="PKZ49_05540"
     CDS             7355..8617
                     /locus_tag="PKZ49_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Na+/H+ antiporter"
                     /protein_id="HND36059.1"
                     /translation="MSDPTFIQLTASILFALAVIHTFSTKYFEHLAHIQPQHAGIWHL
                     LGEVEVVFGFWAMILILFMFAVGGKTEAVGYLNSRDFTEPMFVFVIMVIAGTKPILSF
                     TAFIVQRTANLLPINRAMAMYFLVLALVPLMGSFITEPAAMTLAALMLRDTLFGQTIS
                     NKLKYATVGVLFVNISIGGTLTPFAAPPVLMVAAKWNWDFWFMITTFGWKSAIAVMIN
                     AALVTLLFSKELVRTKQQDTDTTTTKMPALMIAAHITFLTLVVLFAHYPVVFMGLFLF
                     FLGFATAYQRYQNPLILREALLVAFFLGGLVVLGGQQQWWLQPLLMGMDDTAVFFGAT
                     ALTAITDNAALTYLGSLVEGLSEEFKIALVAGAVTGGGLTVIANAPNPAGFAILKSKF
                     DEQAIHPLGLLIAALLPTMIAIIAFRIL"
     gene            complement(8674..11862)
                     /locus_tag="PKZ49_05545"
     CDS             complement(8674..11862)
                     /locus_tag="PKZ49_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HND36060.1"
                     /translation="MMAALVRAILKQRLLVLVMGLMLCVAGGFAVKSLSVDAFPDVTN
                     IQVQVATVSIGRSPEEMERLVTVPVEIAMTGLPGLVEMRSLNRSGLSLITLVFTDQTD
                     VFFARQLVMERIIDVTPRMVPGVTPVLGPVSTGLGEVYQYTIGHPNDGNRQLTFEELM
                     ERRTVQDWVVRPLLRSIRGVAEVNSMGGQVKEYQVYVDPNKLRHYDLTLAQVDRALAS
                     NNANASGNILALRYEQYLIRGVGLIASLEDIRNIVLRELDGVPVYVRDVAEVVFGGEV
                     RQGASIKNGDTESVAGIVMMLRGGNAKEIVGRIKEKVKEVNEKGMLPGGLQIVPFYDR
                     TDLVDSALNTVSTTLMESLILVIVVLSVFLGTVRTSFVVCFTLIITPLVTFLVMNHYG
                     MPANLMSLGGLTIALGMMVDPTVVVVENIYQRLGEAKDTGKSKFNVIVDAVAEVGMPV
                     IFGLIVTVLVFLPLMTLEGMEGKTFSPLAITISISLFIALLVSLLLSPVLCDYLIKGG
                     GEQDTKIIAVMKSAYLSVFDLAMRNQKKTIMIAISSLMLAFAIFPFLGKSFIPIMKEG
                     AITPVIIRAPSISLEEAIAIETEAMKLIAAVPGVKSVVSKLGRGDTPADPAAQNESDP
                     IADLDLKGSGRTQQEIEEDIRKALDGLPGVNIVLSQPIAQRVDEMLTGVRSQVAIKIF
                     GDDLEQLRKLSEQMARIVKSTPGARDIRIERLSGQQELTIDIDRRAIARHGLNVSDVN
                     EIISTAIGGKFVTQVFEGERRFNLLLRFPEGFRHDVEAIKQILLRPANNNIGGEVATG
                     GVLVPLSAVADIHVKDGPAIVSREFARRRVVVGVNVHGRDLGGFVAELQERAAKEIKL
                     PQGYYFVWGGQFENMERAMAKLSIIVPITLAAIFFMLFMLFNSVKLACLIYLVLPFAS
                     VGGVVGLFVFREYLSVPASVGFIAVWGTSILNGVVLISFVKELREKGLSIEESVRKAC
                     SQRFRPVMMTAATTVLGLAPFLTATGLGSEIQKPLAVVVIFGLTTATCMTMIVMPMIY
                     RWFDTPPKRDLADSFEGVTAPAVEAVAQTKKA"
     gene            complement(11866..13035)
                     /locus_tag="PKZ49_05550"
     CDS             complement(11866..13035)
                     /locus_tag="PKZ49_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HND36061.1"
                     /translation="MSIKLSVSSMIFFVLLLSGCSLIDSSDETKKEISQQESTEDRDF
                     NYIKVRPELLKRLEIGQPYLVDLADRIQVPSRVQVDEERTAQIGSYVTGRIIDMFVIL
                     GDYVKPGQRLARITSPDLTQSQLAYLRALSRVTVTQKAYDRAQHLLAADVIPLAEVER
                     RKSELEIAQAEYSAAMDQLRLFGMGEGEIKELNKKGKILPWIDIKSTREGYVIARNII
                     IGQVIQPADPLFQVADLSYVWVVGDVPEQVARDVELGQHVEIIVPAISNNPFDGVIIF
                     VSDIVNRLTRTVMTRVMVENPDRKLKPDMMANMHITDPQHKTLVVPEAAVVRELDQDY
                     VFIALSDDYFQRVPVELGPEVSNLRPVLKGISQGHQIVIEGAFHLDSERKLAELE"
     gene            complement(13032..>13756)
                     /locus_tag="PKZ49_05555"
     CDS             complement(13032..>13756)
                     /locus_tag="PKZ49_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HND36062.1"
                     /translation="VRADTELQSAENRKQAAELTAQRARVALLQFTAGALPINFEVKG
                     SLKDSIDLPTLEELRKEIPEANPEILRLQAEQERTQLRIDQERATVLPQLNVLYTNYQ
                     DRQFTSNTAGLQVRIPLFYQRRGEIDAAIYDSARIRQTLDYRRYEVSQLFEAAWQALQ
                     IAQRRVEMFEGGLIKEAENAVQIAQAAYRFGERGLIEFLDAQRILRGLQGDFLLARFE
                     LQSAAAEIDRLRAHFPKELIPE"
BASE COUNT         4406 a         3066 c         2379 g         3905 t
ORIGIN      
        1 atcacagcga tagcaatggc acatcttgct aagacagtat ctagcttaac gattgaaact
       61 tctgtaaaac ttactggatt tctcgccaag aagagtaaaa taagctcgca gctagtgcta
      121 catgttagtc agatcgatgt aatttaatta tctaagtaca ggaaagagaa aaatggctcg
      181 tttttttaga aacaaagata acaatctgaa agcaaaggaa aagttagcta atcgcccttt
      241 gtttaagcgc aaaaagttct gccgctttac agctgaagga attaaaacta tcgactataa
      301 agatatcgat atattaaagg attttattaa cgagaacggt aagataattc ctgcgcgtat
      361 tacagggacc aagacctttt atcaacgtca gctaaatact gctattgaaa gagcacgttt
      421 tctgtcgctc ctgccctata ccgatcagca ttaaactcaa taaggagaac gattatgcag
      481 atcatactat tagaaaaaat tggcaattta ggtcaactcg gtgacattgt taatgttaaa
      541 aagggatacg ctcgcaatta cttgattcca caaggaaaag caaaatgggc aactgagagc
      601 gcgattgcag aatttaatac taaacgagca gagcttgaaa agaaacaagc cgagaaatta
      661 ggctctgccc aagcccttgc tgcaaaatta aatggcctac tcttacaagt cacacagaaa
      721 agcggtgttg atggaaaatt atttggttcg gttacaagct cgaatattct ggaatctcta
      781 gaagctcaag gcttctctgt tgaaaaatcg atgatacgta tgcccgaagg acaaattaag
      841 caaattggtg attacccaat cacaattgcg attcatcatg atgtgttggc acaaattacg
      901 gtatcggttc taggcgaaac cacagcgtaa cttaaaagct tttatttcta aaagcaggac
      961 tgattagtga acaaacagcc ctgctttttt tatctatcgc ttcagtgata gaataatctt
     1021 cctagtaatc tctgaatttg atgaatgccc atggctcaat cttcaatccc tatcagcatt
     1081 acacacaatg aattactaga tatttataaa actccccccc attcaattga ggctgaacaa
     1141 tccgtactag gtggtttaat gcttgataat caagcatggg ataaaatagc ggacatcatc
     1201 acaattgctg atttttatcg tcacgaccac caactgattt atcatcatat ttgtaatctc
     1261 atcgaacaaa ataaacctgc tgatgtgata accgtggccg agtctttaga taattcggct
     1321 gaattacaaa atattggtgg attagcctat attgggtcta ttgtacaaag cacccccact
     1381 gcagcaaata ttcgccgtta tgctgaaatt gttcgagaac gtgcgatcat gcgtaagctt
     1441 gctgaagtga gcacacaaat tgcagaatct gcctataatc caacaggccg ctcagctaca
     1501 gatttactcg ataatgctga aagtaaaatc tttgaaattg ctgaagctgg cgctagggga
     1561 aaacagggtt ttatcgatat tcagccgtta ctcaaacagg ttgttgaacg aatcgagctc
     1621 ttatatagta aagagaaccc aagtgatgta accggtattg cttcaggttt tcatgatcta
     1681 gaccagaaga cctctggatt tcagcctggt gacttaatca ttgtcgcggg caggccttct
     1741 atgggaaaaa ccgctttttc tctgaatatt gctgaacacg ttgcgctaga attaagcaag
     1801 ccagtagcta tttttagtat ggaaatgggg ggagcgcagc tcgctttaag gatgctcggc
     1861 tcaattggtc gactggatca acataaagta cgaacaggcc gacttgatga tgatgattgg
     1921 ccaaggttaa cgcatgctct gggcaaacta aatgatgccc caatctttat tgatgaaagc
     1981 gctgcattga atgcacttga gttaagagcg cgcgcacgta gactttatcg acaacatgga
     2041 gaactggggt taattgttgt agattactta cagttgatgt cttcacactc acaaaatgaa
     2101 aaccgcgcaa cagaaatttc tgaaatttca cgctctctga aaggactagc taaggaactt
     2161 caagttccct tgattgcact atcacagcta aaccgaggct tagagcaacg acccaacaag
     2221 cggcctatta tgtctgactt aagggaatcg ggtgctattg agcaagatgc agatgtaata
     2281 ctttttattt atcgtgatga agtatataac cctgacacac aagacaaggg ggttgctgaa
     2341 attattattg gtaaacagcg caacggccca attggaaagg tcgatctaac tttcttaggg
     2401 gaatacacgc gctttgaaaa ttatgcgaaa ccggattatt attaatatag tttgatttcg
     2461 taaattgcca ctacaaaaca cattttcttg cggttacttg aatttcaatt cgcattctcg
     2521 ggtctgataa tcccgctgaa atcatcattg ctgctggacg aacatctcca agatatttac
     2581 ggaaaatagg ccagcatttc tcaaaatcag aagcctctgg caatacataa gttaccctta
     2641 caacatcttt taaacttgaa ttagcctgct tcaatgccag ctcgatattt ttaaaacatt
     2701 gctcagcttg ttctaataaa tcttccgaaa tactcatggt tgaataatca aaaccagtcg
     2761 ttcctgacac aaaaatccag tctccctcaa caaccgctct tgaataacca atctcttctt
     2821 caaatgttga accagaacta attaatcgcc ttgtcattaa gtttcctcta cttctcaaaa
     2881 taaaattatt atttcgcgat tatacaagta aagatttacc ttgatacagt attcagtaag
     2941 attaaatctg cttaaccttg ttaaatttat tgttattaag caaaagctgg taggcataat
     3001 aaaatctctc taaccagcaa tacctatttc ataaaatgat cagcagtata attcttaaag
     3061 aactagaggg gtatcaccag gagataactt tcagcaagtg cgcgatctat tactaaattt
     3121 ttattttgat aagccgtaac actttataag ttaatcagta caaaacgtac taatttagat
     3181 tctctcgtaa aaggatatac taatgtcggc agacaagaac ttaaaatttt tagtagttga
     3241 tgatttctct accatgagac gaatcatacg gaaccttctc agggaattag gctttcttaa
     3301 tgtagaagaa gcggaagatg gagctgcagc tctaagaaaa cttcgtgaaa gtagttttga
     3361 ttttgttgtg tccgattgga acatgcccaa tatggatgga cttacaatgt tacaaagtgt
     3421 tcgagcagat gaagaactaa aacaaattcc tgttttgatg gtaacagctg aggctaagaa
     3481 agaaaacata attgcagccg ctcaagctgg tgcgaacggt tatatcgtta agccttttac
     3541 tgcagcaact ctagatgaaa aattaaataa aatcctacag aatattgctc ataaagcagc
     3601 ttcgtaaaga aagcctactt taccgatcga tttaagaaag caatgaataa tcctactaac
     3661 tcttcaccaa ctgaaatatc agctgatgat aagctacttg aaagaatagg aaaacttaca
     3721 agactccttc ataacagcat ttgtgaactt ggatatgata aagataaaaa attacaagaa
     3781 gctagtgagc aaatagcaga tatccatgaa agattgaatt atgttttgtc taaaacacaa
     3841 tcagcagctg aaacaacact gtccgctatc gaaaatatta aaccaataca atcaaatcta
     3901 tctagtagtg caattatctt aactcaagaa tggcaagagt taattaaact cgaaaacagc
     3961 tgtcattcaa ataaaaatgt agcgaaattt agcaatttat tcatgagaac agtatcttac
     4021 ttagaaaata tacctaaaca agcagaagta actaatgatt acttagtaac tgtaataatg
     4081 gctcaagatt ttcaggattt aactggtcaa gtaattaaaa aaatttctca tttactgctg
     4141 ctaatcgaaa atgaattaat acaggtatta ctagataact caatatcaac caaccctgca
     4201 accataaata acaaaagctc tccaaacaat ttactcaacg gacctaccat caatcctcaa
     4261 aaaaaacctg agatcctctc taatcagact gatgttgata acttcttagc agacttaggt
     4321 tattaaagaa ataaaagaat tcaagttatt ttatttcatt tttgtaaagc tggtaaactc
     4381 actatgaagt tttctaactt gatctactaa ataaataaaa tcccgatcat tagaaatgat
     4441 attgttagct ctggccaaac gttcttttct tgagatttgc gtcttaatta tcgctcgaac
     4501 atctttctgg gaaagcttac ttcgctgaac tgttcgtaaa atttgtaact cttctggaca
     4561 atcaacaacg aggactcgat cgattaaatt acaaaagcaa cccgtctcga acaacagtgg
     4621 tatagctaca atcccatata atgactcaat cgttggaata agttgttcta catttctata
     4681 aataagcgga tgcaatattt cctcaagctt ctttttcata gataaatctg taaatacaag
     4741 atttctcatt tttaccctat ctaagcgccc agtatcagta atgtattctc ccccaaaaat
     4801 ttcctttata tgagaaattg cttctccatt ctcatgagtt aactcatgag caatttcatc
     4861 agtatcaata atggtaatac ccaatgtttg aaaaatctca gcaacactgg tttttccaga
     4921 accaatacca ccagtcaatc caacgatcaa gggtaattcc ttctagaaca gactgagata
     4981 gctatgatta atttgctttc cccagaacat tgcaattaat ccacctcccg caagataggg
     5041 tccaaatgga attgctaatc cgctatgctg atttcgaaaa taaattagaa aaataccaac
     5101 taaaccacca acaaatgatg aagataaaat aacgaggggc aatgtctgcc acccaaacca
     5161 ggcgccaatt gcagcaagaa gtttaaaatc accgtagccc atcccttctt taccagtaat
     5221 tagtttaaaa atccagtaaa ctacccaaag tactagataa ccggctacag caccaataac
     5281 tgctgactgg agatcaataa tcccattatt tagattaatc agaagaccaa tccacaataa
     5341 tggcaatgtg attgcatctg gcaagagctg tgtatcaaaa tcgataaacg taagtgcaat
     5401 catagcccaa ataaaaataa gagcagcgaa acacataaaa ctatacccat aataccatgc
     5461 agcaaaacca ctcattaatc ctgctaatag ttcaactgtt gggtaacaaa aagaaatatg
     5521 ctcatgacat tgagaacagc gacctcttaa aaacaaatag ctaataaggg gaatattttc
     5581 aagcattgtg atcttgtgac cacagtgagg acaattagat cttggcgtaa taagattaaa
     5641 tacgggtaat aatttagcgg gttccccctt caattccgca cattgctgat gccaagcaag
     5701 ctccatcatt ttgggtagtc gataaataac gacatttaaa aaactaccaa ctgtaattcc
     5761 tagaactcca caaattgtta caaataattc tggcgatgct tgaaaggaat ttataaatgc
     5821 catgatatta ataggtcaat aaaacaaaca aattaagttt agccaacgac ttgccccatt
     5881 ttgaaaatag gtaaatacat tgccacaacc attcccccaa ttaatgtacc taaaacaacc
     5941 atgatcatag gctccattaa gcttgataaa gcttccactg cattatctac ctcagcctca
     6001 aagaaatcag caaccttaga taacatcgca tccaaagaac cagcctcttc accaatcgca
     6061 accatctgaa gtaccatact cgagaatacc tgagtttctg tcatggcagc aactaaacta
     6121 ctccctgtac ttacttcact ttgaatatgt ttcgtggcct cataatagac gtaattacca
     6181 gctgcccctg caactgaatc aagcgcttct accagcggaa cccctgcagc gaacattgtc
     6241 gataacgttc gtgaccagcg tgcaacagtt gctttcctaa tcacatctcc aaaaataggg
     6301 agccttagca ataacttatc cataacacgt tgcattgcaa tggatcgttt ccaggcataa
     6361 aagaagctat ataaactggc accgatccca ccaaaaatag cccaccaata gtcaacaaag
     6421 aaatcagaaa ttgtcataac cattaatgta ggccccggca aatctgctcc aaacccttga
     6481 aatagttctt tgaatgcagg aataacaaaa atcataatca ctgctgtaat cacgaacgca
     6541 acaacaataa ttgagattgg ataaaataat gcagacttaa ttttaccttt tatagcctga
     6601 atcttttctt tatacgtagc taaacgatcc agcaaactat ccaatatacc cgcagcttca
     6661 ccagcagcaa ctaaattaca gaataaagaa tcgaaataaa ggggatattt tcggaacgca
     6721 ttggctaagc tacttcctgt ttcaacatca agcttaatat ccattaatag tttgcccact
     6781 gccggattac tatgcccctt accaacaata tcaaaagctt gcaacaaagg aacccctgat
     6841 ttcatcatcg tagctagttg ccgagtaaac aacgttatat ccttatctga aattgatcca
     6901 ctggattttg ttttagcaac tttagtaaca cgaatacctt ggcgccgtag cgtggaattt
     6961 acaacctcaa cacctgctgc cctcatttct cccttaatta atttcccagt tttatccttc
     7021 ccttcccaag taaaactaat ttctttgact tttttgtcat tacttgcggc aacagttgcc
     7081 ataattactc cttatcatac ccaaatgtta gtgattcaaa tcaaccttga tcaaaacaaa
     7141 gctttgatag attaaacttg taatctataa aattacggca aatcctgaga atgagcttag
     7201 atcaaacaaa taaggtttaa ggtagctttc gttaaaataa acagaaaatc aatataagta
     7261 atcagtattt tacagttaac tacgcactgc tctatcattc ataatcattt tcctgcatga
     7321 tgcgtatcca ttatctctaa ataataattc aatgatgagt gacccaacat ttattcaatt
     7381 aactgcaagc atcttatttg cacttgcagt aattcacact ttttcaacca aatattttga
     7441 gcatttggct catattcagc ctcaacacgc tggaatatgg catttactcg gagaagttga
     7501 agttgttttt ggtttctggg caatgattct aatactcttt atgtttgctg tcggtggaaa
     7561 aactgaagct gtgggttact taaattcacg tgatttcact gaacccatgt ttgtgtttgt
     7621 aatcatggtc attgcaggca caaaacctat ccttagtttc acagccttta tagtgcaacg
     7681 gactgcaaat cttctaccaa tcaatcgtgc tatggctatg tatttcctgg tactcgccct
     7741 tgttccctta atgggctctt ttatcacaga accagcagca atgacgctag cagcgcttat
     7801 gctacgcgac acattatttg gccaaaccat atcaaataaa cttaaatatg ctacagtcgg
     7861 tgtactgttc gttaatatct ctattggagg aacgttaaca ccttttgcag ctccccctgt
     7921 attaatggtt gccgctaaat ggaattggga tttctggttt atgattacaa cttttggctg
     7981 gaaatcagcg attgcggtta tgataaacgc tgcacttgtt acattacttt ttagcaaaga
     8041 gctagtaaga acaaagcagc aggatacaga cactaccact acaaagatgc ctgctttaat
     8101 gattgctgct catatcactt ttctaacact cgttgtatta ttcgctcatt atccagtagt
     8161 attcatgggg ctattcctat tcttcctcgg atttgcaacg gcatatcaac gctaccaaaa
     8221 tccactcatt ttacgcgaag cgctattagt ggcttttttc ttaggaggac tagtcgttct
     8281 tggtggtcag caacaatggt ggctacaacc attattaatg ggcatggatg acactgcagt
     8341 attttttggc gcaactgccc taaccgcaat tacagataat gccgctttga cttatttagg
     8401 ttcattagtt gaaggtttga gtgaagaatt taaaattgct ttagttgcag gggcagttac
     8461 tggtggaggc ttaaccgtaa ttgccaatgc ccctaacccg gcaggatttg caatcttaaa
     8521 aagtaagttc gatgagcagg cgattcatcc attaggttta ctaattgctg cattacttcc
     8581 aacaatgatt gccattatcg cttttcgcat actctaggct atcaacaata ggactgaata
     8641 aaataaattc agccctatcc gatttataca gaattatgct ttcttagttt gagctaccgc
     8701 ttctacagct ggtgcggtta ctccctcaaa ggagtccgct aaatcccttt taggcggtgt
     8761 gtcaaaccaa cgataaatca tcggcatcac aatcattgtc atgcaagttg ctgtcgtcaa
     8821 tccaaatatt acaacgacag ctaggggttt ttggatttca gaacctaacc ctgtcgcggt
     8881 aaggaatggt gctaatccta gcaccgtagt agcagctgtc atcataacgg gtcggaaacg
     8941 ttgggaacag gccttcctaa ccgactcttc aatactcaag cctttttcac gtaattcctt
     9001 cacaaaagaa atgagcacaa ccccatttaa aatagaagta ccccacactg caataaatcc
     9061 cactgatgcc ggcactgaca aatactctct aaaaacaaat aacccaacga ctccaccaac
     9121 cgatgcaaag ggtagtacta aataaatcag acatgccagc ttaactgaat taaacaacat
     9181 aaataacatg aagaaaatag cagcgagcgt aattggaacg atgatgctaa gtttagccat
     9241 agcacgctcc atattctcaa attgcccccc ccaaacaaaa taataacctt gaggtaattt
     9301 aatttccttg gcagcacgtt cctgcagctc tgcaacgaaa ccacccaaat cacgaccatg
     9361 aacatttaca ccaacaacaa cacgacgacg cgcaaattct cgtgaaacaa ttgcaggccc
     9421 atctttgaca tgaatatcag cgacagcact taatggaact agcacaccgc ccgtcgccac
     9481 ttcaccacct atgttattat tagctggtcg caatagaatt tgtttaattg cttctacatc
     9541 atgacggaat ccctctggga atcgcagtag cagattaaat cgacgctcac cttcaaacac
     9601 ctgtgtaaca aatttaccgc caatggctgt tgaaatgatt tcattgacat cggaaacatt
     9661 cagtccgtgc cgtgcaattg cacgacgatc aatatcaatg gtcaactctt gttgaccgga
     9721 taagcgttcg attcgtatat ctcttgcacc aggtgttgat ttaacaatcc gcgccatttg
     9781 ctcagaaagt ttgcgcaatt gttccaaatc atcgccaaag attttaattg ccacttgaga
     9841 tctcacccct gtcaacattt catctacccg ttgtgcaatc ggttgagaaa gtacaatatt
     9901 taccccgggt aatccatcca atgctttacg gatatcctct tcaatttctt gctgagttcg
     9961 accagagcct tttaaatcta aatcggcaat tggatcggac tcgttctggg ctgccggatc
    10021 agcaggtgta tcaccacgac ctaatttaga aacaacactc ttaacacctg gaacggctgc
    10081 aatcagcttc attgcttcag tttcgatcgc aatcgcttcc tctaaagaaa tagaaggtgc
    10141 gcgaatgatt accggcgtta ttgccccctc cttcataatc gggataaaag atttccccaa
    10201 aaaaggaaaa atagcaaaag caagcatgag tgagctaata gcaatcatga tggttttttt
    10261 ctgattacgc attgcaagat caaaaacact caagtatgcg cttttcatca ccgcaataat
    10321 tttagtatct tgttcacctc ctccctttat taagtaatca cacagcaccg gcgataataa
    10381 taatgatacg agtaatgcga taaacaaaga aattgaaatt gtaattgcaa gcggactaaa
    10441 cgttttccct tccattcctt ccagtgtcat aagtggaaga aacactagca ccgttacgat
    10501 aagtccaaaa atcacgggca ttccaacctc tgccacagcg tcaacaataa cattaaattt
    10561 agatttaccc gtatctttgg cttctcctaa gcgttggtag atattctcca caacaactac
    10621 cgtaggatcc accatcattc ctaatgcaat ggtcaatcca cccagcgaca taagatttgc
    10681 aggcatacca taatgattca tgactaaaaa agtcactagc ggtgtaatga ttagggtaaa
    10741 acacacgacg aaactagtgc gcactgttcc tagaaaaact gataacacga caataactag
    10801 aatcagtgat tccattaatg tagttgaaac cgtgttcagt gcactatcca ctaaatcggt
    10861 tcgatcataa aacggaacaa tttgtaaccc cccaggtaac atccccttct catttacttc
    10921 cttaactttt tctttaattc gcccaacaat ttccttagca ttacctcctc gcagcatcat
    10981 tacgatgcct gcaaccgatt cggtatcgcc atttttaata ctagcgcctt gccgcacttc
    11041 accaccaaaa accacctcag cgacatctcg gacataaaca ggcacgccat ccaactctct
    11101 gagaacgata ttgcgtatat cctctaaact ggcaatcagc cctacacccc gaatgagata
    11161 ttgctcataa cgcaatgcta aaatattccc gctcgcgttc gcgttattac tcgctaacgc
    11221 tcgatcaact tgagcaagtg ttaagtcata gtgccgcaat ttgttgggat caacgtaaac
    11281 ctgatactct ttaacctgtc ctcccataga attaacttct gcaacacctc gaattgaccg
    11341 aagcagtggc cttacaaccc aatcttgaac tgttctccgc tccatcaact cttcaaaagt
    11401 tagctgacga ttcccatcat tggggtgccc gatcgtatat tgataaactt ccccaagccc
    11461 tgttgatacc ggccctagca ccggtgttac cccaggaacc atacgaggtg taacatcgat
    11521 aattctttcc atcactaact ggcgtgcaaa aaacacatca gtttgatcag taaataccag
    11581 cgttattagt gataatccac ttctattcaa ggagcgcatc tcaaccagac caggaagtcc
    11641 tgtcatggct atctctacag gaacagttac gagtcgctcc atttcttcag gactgcggcc
    11701 aatagaaacc gtggcaactt gcacttgaat attcgttaca tcaggaaaag cgtctactga
    11761 tagagattta actgcaaacc caccagcaac acatagcatc aaacccatta ctaaaacaag
    11821 caagcgttgt tttagaattg cgcgaactaa agctgccatt atgaattact ccaattctgc
    11881 aagcttgcgc tcactgtcta aatgaaaagc gccttcgatc acaatctgat ggccttgtga
    11941 aattcctttc agtactggac gtaaatttga tacttcgggc cctagttcta caggaactcg
    12001 ctgaaaataa tcatcactca atgcaataaa aacatagtcc tggtctaatt cgcgaacgac
    12061 ggccgcttcg ggaactacca gagttttatg ctgtggatct gtaatatgca tgtttgccat
    12121 catatctggc ttaagcttac gatctgggtt ctcaaccatc acacgggtca tgaccgtgcg
    12181 cgtcaaacga ttaacgatat ctgaaacaaa gatgatgaca ccatcgaaag gattattgga
    12241 tatagcagga actataatct cgacatgctg acctaattcg acatccctcg ctacttgctc
    12301 aggcacatca cctacaaccc atacatacga taaatcagct acttgaaaca gaggatcggc
    12361 aggttgtatt acctgcccga taataatatt acgtgcgatc acataacctt cccgggtaga
    12421 ttttatatct atccagggta agattttccc ctttttatta agctccttga tttctccttc
    12481 ccccatccca aacaatctta attgatccat agccgcactg tactcagcct gtgcgatttc
    12541 aagctcagat ttcctccgtt ctacttcagc aagtggaatc acatcagctg ctaataaatg
    12601 ctgtgcacgg tcatacgctt tctgcgtgac ggtaactcgc gataaagcgc gtagataagc
    12661 tagttgcgat tgtgttaaat caggactagt tatccgtgcc aaacgttgtc caggttttac
    12721 ataatcacct aatattacaa acatatctat gatacgaccg gttacatagg aaccaatctg
    12781 tgctgttctc tcttcatcga cttgtactcg actaggcact tgaattctgt ctgcaagatc
    12841 cactaaatag ggctgaccaa tttcaagtcg tttaagcaac tcaggcctta cttttatata
    12901 gttaaaatct ctatcctctg ttgattcttg ctgcgatatt tcttttttgg tttcatcact
    12961 cgagtcaatc aaactacaac cacttaacag tagtacaaaa aaaatcatgc ttgaaactga
    13021 caatttgata ctcattctgg aatcaactcc ttaggaaaat gtgcacgcag acgatcaatc
    13081 tcagctgcag ccgactgcaa ctcaaagcgg gctaataaaa aatccccctg aagcccacgc
    13141 agtattcgct gagcatctaa aaactcaatc aaacctcgtt caccaaatcg ataagcagct
    13201 tgtgctattt gaactgcatt ttctgcttcc ttaatcaatc ctccctcaaa catttcaacg
    13261 cgtctttggg caatttgtag cgcttgccaa gccgcttcaa ataattgact tacttcatag
    13321 cggcgataat ccagggtctg gcggatgcgt gccgaatcat agatcgctgc atcaatctca
    13381 ccgcgtcgtt gatagaaaag tggaatcctc acctgcaaac cagcagtgtt ggatgtgaat
    13441 tggcgatctt gatagttagt gtacaaaaca ttcaattgtg gcagaactgt cgctcgctct
    13501 tgatcaatcc gtaattgagt tcgctcttgt tccgcttgta aacgcaatat ttctgggtta
    13561 gcttcaggaa tttcttttct caattcttct agcgtcggta aatcaataga gtcttttaga
    13621 gaacctttaa cttcgaaatt aatcggtaat gcccctgctg taaattgcag caatgcaacc
    13681 cgcgctcttt gtgcagtcaa ttctgcagcc tgcttacgat tttcagcact ttgtaactca
    13741 gtatctgctc gaacta
//
LOCUS       DAOQKQ010000061        13456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_2155_length_13456_cov_5.231102, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000061 DAOQKQ010000000
VERSION     DAOQKQ010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 13456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13456
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2155_length_13456_cov_5.231102"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..2347)
                     /locus_tag="PKZ49_05560"
     CDS             complement(140..2347)
                     /locus_tag="PKZ49_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HND36063.1"
                     /translation="MNSDLNDSHPSDTDDHRQINSKLPDAPLAADEDKWTCPMHPEIL
                     RNAPGDCPICGMALIPITNTNMGESDNSELRDLTRRLWIGIALSLPLIALTMMPMIGL
                     NEPFGLQPRLRGWIEFLLGTPVVLWVGWPILRKFWLSLVHRALNMYTLIGLGVGCAYF
                     FSLAAVLMPDWFPQEFHAHDGAVGTYFEAAAVIVTLVILGDYLQLRAMGQTSQAIQQL
                     LRLAPNQAWRLRDDGTEEQVPLDVIVVGDRLRVKPGEKIPVDGTVLEGMSRVDESMIT
                     GEPMSVAKAPGDKITGATMNSNGSLIIRAEHVGADTLLARIVHMVGEAQRTRAPIQRL
                     ADIIAAYFVQIVIAIAIITALAWWFLGPEPKFTYAFLNAIAVLIIACPCAVGLATPIS
                     MTVAMGQGARMGILFRNAEAIERMRDIDTLVIDKTGTLTLGHPVLTNFIVKGIADDEA
                     LALIASVEQLSEHPIGLAIVENAKARGLTLRPAKSFDAINGLGVQADIDGEYVLVGNY
                     SFLAQHGVDTQPWHHQAEALRTEAKTVVFFAVDGIPVGIAAVADPIKENTPEAIVTLK
                     HLGIRIVMLTGDSQHTANAIARHLGIDEVLAEVLPEDKAGHVKRLQAEGRKVAMAGDG
                     INDAPALAQADVGIAMGTGTDVAMESASITLIKGDLRGIERAILMSHATMRNIHQNLA
                     FAFGYNVLGIPLAAGVLYPAFGFLLSPMFAGAAMALSSVSVVTNALRLNRTRI"
     gene            complement(2414..2767)
                     /locus_tag="PKZ49_05565"
     CDS             complement(2414..2767)
                     /locus_tag="PKZ49_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36064.1"
                     /translation="MKARIFFATLVILASLISCAQMNPHPMEMTSAVRNAKTSADHEA
                     LAKHYEDAVHEMQLKVEEHKKLLEEYEKHPYYYPKRAQDLQGHCWSLIKSYEQAVEAN
                     MSMAKIHHQIADEVK"
     gene            complement(2949..3557)
                     /locus_tag="PKZ49_05570"
     CDS             complement(2949..3557)
                     /locus_tag="PKZ49_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HND36065.1"
                     /translation="MFNLLAETLVNALDLREHETGLHSKRVACHTMVLARRFTSDPNL
                     LQQIYWGTLLHDIGKIGISDAILLKAGSLTESEWIEMRTHPEKGYHLISQIPAMTDAA
                     NIILCHEERYDGSGYPKGLSGGRIPLGARLFMVIDALDSITSNRPYRKSHSFEFAKAE
                     ILRMKGSQFDPEAVDAFSAEEEVLQKMVIAKCHNHPSLVSSR"
     gene            complement(3766..6468)
                     /locus_tag="PKZ49_05575"
     CDS             complement(3766..6468)
                     /locus_tag="PKZ49_05575"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HND36066.1"
                     /translation="MNAWHSQSIAELEHLIATDVERGLTDEEVVDRLVRNGPNKLRKG
                     KRFSAFAILASQFKSLVIWVLIGAAAVSTALGETVDGIAIIAIVILNALIGFIQEYRA
                     EKAAAALADMVAPHCRVVRNGHSVVVAATEIVPGDILLLEGGDLVTADARLIQASVLR
                     INEAPLTGESQAVNKFTDNLPPETALAERKNMVFLGTSVTGGSGRALVVNTGMETELG
                     HIAKLLETAESGETPLQVQLDRVGHMLLLACFSIVALIFGLGLLRGIAPFELFLSTVS
                     LAVAAIPEGLPAVVTIALALGVQRMVRRNALVRRLASIETLGRAQVICTDKTGTLTMG
                     EMTACKLITADNLYRIPGEGYSTEGGFFVGNAEIPVEGTPSLLALLRASAACNDAELA
                     LIDGRSMIVGDPTEGALLVAAAKGNITRQMIEAEMPRLAIVPFDSARKRMTVIRRQEN
                     NSWAFAKGAPEVILGRCTRIRTDQGIRDLTESDRVRLLHANTLLANDALRVLAIAERI
                     LDGFSFEEGKVTDDAEIEKELIFLGLIGLQDPPRAEAKEAVAKCKRAGIKTVMITGDH
                     PDTARAIGRELGILGKSDEILAGIELDRLNDEVLKERVSRVSVYARVTAEHKLRIVRA
                     WKSQSAVVAMTGDGVNDAPAIKEASIGIAMGVTGTEVTKEAADIIITDDNFASIVAAV
                     EEGRGIYDNIAKTLAYLLGSSTGELIVMLVAVLLGWPLPLLPLHLLWINLVTDGFAAL
                     ALSTDPVDSDVLDRPPRHPQTALINRDLIKLTLFTGLLTASVTLGVFAYELYIMGSSL
                     EQARDAAFTTLVIAGLLRAFGARSEQRAIWEISMFSNIRLFLVVAVCFGLQLAIHHVP
                     MFQTLFGVEPITLSQCIGWFAVGFIPLIVLELRKFIRYRLIKEI"
     gene            complement(6465..7055)
                     /locus_tag="PKZ49_05580"
     CDS             complement(6465..7055)
                     /locus_tag="PKZ49_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HND36067.1"
                     /translation="MRRLFPESGIKTALIAQARIQPSQNVLDVGCGTGTLTLMIKQIQ
                     PDTGENGLDMNPQILDIARRKAEQAGEPIVLQQGTATCLPYLNESFDHVFASLMLHHL
                     TREDKQQALREAFRVLKPGGELHIADFGKPHDSVMRLISLVMRWAEEVHDNILGLLPV
                     FIADAGFHPVEETTRYRTVVGAIALYRACKPMRNKL"
     gene            complement(7121..10270)
                     /locus_tag="PKZ49_05585"
     CDS             complement(7121..10270)
                     /locus_tag="PKZ49_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HND36068.1"
                     /translation="MVGNIIEWSVRNVFLVLLGTFFVVAWGVYAVLKTPIDAIPDLSD
                     VQVIIYTEYPGQAPQVVEDQVTYPLTTAMLSVPKSKVVRGISNFGVSFVYVIFEDGTD
                     IYWARSRVLEYLSFAANRLPENVRPSLGPDATGVGWVYQYVLVAKNHALDELRAIQDW
                     FLRYQLTATHGVSEVASVGGFVKNYQVTVDPRRLQAYGISLKTISEVIASSNRDVGGR
                     VIELTETEYMVRGKGYLRGISDLENLVVKAHEGMPVLLSHVARVELVPDERRGITEFN
                     GEGEVVSGIAVARHGENALNVTHNLEKKIDEIAPGLPEGVSIQPVYNRSELIHRAIAT
                     LNEVFIEQIIIVALVCVVFLMHVRSALVAIIMLPIGVLIAFIGMFHLGINSNIMSLAG
                     IALAIAEMTDAAIVMVENAHKHLARLAPDESRTEAVIAACKEVGPPLFFSLLIITVSF
                     IPVFVLEAQEGRMFHPLAYTKTFAMAGAALLSITLVPALILLLLRGRIPREQDNPLGR
                     IMIQLYRPVMARVLQWKKVIVMVAIVVVGITVYPALKLGTEFMPTLNEGTLLYMPVTL
                     PGISVTKAGEILQTQNKIIKSFPEVASVLGKAGRANTATDPAPLEMTETIINLKPESE
                     WRPGMTIDKLIAELDQVLQMPGVSNAWTMPIKNRNDMLATGIRTPVGIKVFGKDLGEI
                     EKLAKQIEAAVKTVPGTSSAYAERTTGGYYLDVEPDRLELARYGLAVGDLLSVISAAL
                     GGEMVTTTVEGRERFGVTVRYPRELRSDPQAIATQVLVPTMGATMIPLGQLARISLVK
                     GPPSIRTENALLSAYIFVDIRDRDIGSYVTAGQKAVREQVQFPPGYYATWSGQFEYME
                     RASEKLKVVVPITLLMVFLLVYLNFNRLTETLIVMLSVPFSLVGGIWLMYWLDYNMSI
                     AAAIGFIGLVGIAAESGMVMLEFLDQAFKDVTARREAAGSKVTVEDLYEAVVQGAVYR
                     IRPVMMTIAGSVVGLLPVMFSSGTGSEVIRRISAPMVGGMVSATILTLIVFPAIYALV
                     KEISIRHSLKAEKMGGLSS"
     gene            complement(10272..11864)
                     /locus_tag="PKZ49_05590"
     CDS             complement(10272..11864)
                     /locus_tag="PKZ49_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HND36069.1"
                     /translation="MKSVVKLIMVGVAITTALFASYWWGSTQSQRSPGVALSETPNTE
                     KKILYYRNPMGLPDTSPVPKKDPMGMDYLPVYEGEESPSDQTIVKISTEKIQKLGVRT
                     ETATLRELTRTIRAVATIQADERKLYTLVTKFEGWIQRLYVNTTGQVVKKGDALMDVY
                     SPDLITAQQEYLIAIRGLQSTADSDTDVRSSMQRLVASALQKLRNWDIAETELQRLQQ
                     TGEVRQYMTLRSKADGVVLEKKAVMGQRFMPGEVLYQIVDLSSVWMLADVFEQDLGMV
                     HQGQVATIRVDSYPNKVFNGEIAFIYPTVTPETRTAIVRVVLPNPDGLLKPAMYARVE
                     FASSHSKDKVLAIPDSAVLDTGTRRVVLVDLGAGRFEPRTVKLGMHADGYAEVLGGIN
                     AGETIVVKANFLIDAESNLKAVLSGFGHGGHQQPDEKKETGTGSFVKTSPSKTHRGEG
                     TINAIDFSHATVTLAHGPITSLQWPSMTMDFHVSDPALLRSLKLGQQVIFEIAEESAG
                     EYAIVHIQPADSSLGTNAHGGH"
     gene            complement(11889..13235)
                     /locus_tag="PKZ49_05595"
     CDS             complement(11889..13235)
                     /locus_tag="PKZ49_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HND36070.1"
                     /translation="MIPGRIVAAVLAVIVLPANAQGKEDILNKAGTAIFAQSATKATL
                     TASNSHNVTLLPSLIAEALENNPEIQAAYQEREAAQQRVSPAEALDDPMLEAGVINAP
                     LASSPFNREDMTMKMIGLSQRLPFPGKRGLRKDVAAKDAEAIEQGYHETVNRVVHDLK
                     TAYFDLGLTLEMIKLAEKNKQTLERFLRIAEERYQVGQGSQADVLKAQTQVSRMLDRL
                     LVLAREQPVFEAELIRTLGRNTKGEIPIPVPLQIQEVPLNLETFQQEAIAQRPQLLAL
                     QSLIARNEKSIDLARRAYYPDFDVRLSYGQRDNMMDGTRRDDMVSMTVAVNLPVWRGN
                     KIEPRIMESQALRDQTISLYQAQTNEITARLRQQIAIAEQSLKSVKLYQTAILPQAKL
                     TVESALAAYQVNRVDFLTLLDNQMTVFDFEANLITAMANYNKAVAEIDLLAGKKQH"
     gene            complement(13232..>13456)
                     /locus_tag="PKZ49_05600"
     CDS             complement(13232..>13456)
                     /locus_tag="PKZ49_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HND36071.1"
                     /translation="VVLQVDQSNATIKINHDPIAALNWPRMTMPFRLKERSLATKIKE
                     GDIVEFSLEKEASNYVIVKLSKQNSTGSSQ"
BASE COUNT         3428 a         3501 c         3048 g         3479 t
ORIGIN      
        1 ttatatatcc ttactaatta tgatcaatta acaccctatc aaaaacagta tatatcatca
       61 taggttacag tacagcataa aatttagtag cataagcaaa tgaaaaataa aaaccaatat
      121 ccaaataatt tcctagtaat catattcttg tacgattaag cctcaacgcg ttagtaacaa
      181 ccgaaaccga gcttaatgcc atggctgcac cggcaaacat gggtgaaagt aaaaaaccaa
      241 aagccgggta caatacaccg gcagcaagcg gaatgccaag gacgttatag ccgaatgcaa
      301 aagcaaggtt ttggtgaata ttacgcatgg tggcgtgcga catcaggata gcgcgctcaa
      361 taccgcgcag atcccccttt ataagggtaa tacttgcgct ttccattgca acatcggtgc
      421 cggtacccat cgctataccg acatcggcct gcgctagcgc aggcgcatca ttgataccat
      481 cacccgccat cgcaaccttg cggccttcgg cttgcaaacg ttttacatgc ccagccttgt
      541 cttcgggaag cacttcggcc agcacttcat caatcccgag atgccgagct attgcattgg
      601 ctgtgtgctg ggaatctccg gtcagcatca caattctaat gcccaaatgt ttgagcgtta
      661 cgatagcttc tggcgtgttt tccttaatgg gatcagcgac tgctgcgatt ccaacgggaa
      721 tgccatccac ggcgaagaac accactgttt tggcttcagt gcgtagagct tctgcttgat
      781 gatgccaagg ttgagtatcc acgccatgtt gcgcgagaaa gctgtaatta ccaacgagca
      841 cgtactcgcc atcaatatct gcttgtacgc caaggccatt gattgcgtcg aaagattttg
      901 ctggacgcag agtcaaacca cgtgcttttg cattctctac aattgcaaga ccaatcgggt
      961 gttccgaaag ttgttctaca cttgcgatca gcgcgagcgc ctcatcgtcc gcaataccct
     1021 tgacgataaa attggttaga accggatgcc cgagtgtcag cgtgccagtt ttgtctatca
     1081 ctaaggtatc aatatcccgc atgcgttcga tcgcctctgc attgcgaaac aggataccca
     1141 tccgagcgcc ttgtcccatc gcgacggtca ttgaaatagg tgttgcaaga ccgaccgcac
     1201 aagggcaggc gatgatgaga acggcaatgg cgttaagaaa tgcataagtg aattttggct
     1261 cggggcctag aaaccaccat gctagtgcag tgataattgc gatggcaata acgatctgaa
     1321 caaagtaagc ggcgatgata tccgcaagcc gctggatagg cgcgcgcgtg cgttgggctt
     1381 ctccgaccat gtgaacgata cgtgccagca atgtgtctgc gccgacgtgt tcagcgcgga
     1441 taatcaatga gccattacta ttcatggttg caccggtgat cttgtcgccc ggtgctttag
     1501 ctaccgacat aggctcaccg gtaatcattg attcgtcaac tctgctcatg ccttcaagta
     1561 ctgtgccatc gacaggaatt ttttctccag gttttacgcg tagcctgtca cctacaacaa
     1621 ttacatcaag cggtacttgt tcctccgttc cgtcatcgcg caagcgccaa gcttggttgg
     1681 gtgcaagcct caacaattgt tgaattgcct gacttgtctg acccatcgca cgcaactgaa
     1741 ggtaatcgcc aagtatcaca agtgttacga taacagctgc tgcctcaaaa taagtgccca
     1801 ccgcaccgtc gtgcgcgtga aactcctgtg gaaaccagtc aggcataagt actgcggcca
     1861 gactgaagaa ataggcacat ccgacgccga gtccgattag tgtatacata ttgagcgcgc
     1921 ggtgtacgag tgagagccag aacttgcgca ggataggcca acccacccac agcactactg
     1981 gcgtacccag caggaattcg atccaaccac gtaaacgagg ttgaaggcca aatggttcat
     2041 ttagaccaat catgggcatc atcgtgagag caatcagtgg aagcgacaaa gcaatgccaa
     2101 tccacaaccg tcgcgtgaga tcgcgaagct cggaattatc cgattcgccc atattcgtat
     2161 ttgtaatagg tatcagagcc ataccgcaga tcgggcagtc tcccggtgca ttccgcaaga
     2221 tttccgggtg cattggacat gtccatttat cttcatccgc tgctagcggc gcatctggaa
     2281 gcttggagtt tatttgccga tgatcatcag tatctgacgg atgtgaatcg tttaaatctg
     2341 aattcatttt gaccctccca taaatcatat ctaacctgaa ggctacgagt ctgtcgttct
     2401 taagttgtgg acattacttc acttcatccg ctatttggtg gtgaattttc gccatgctca
     2461 tatttgcttc tacagcttgc tcataacttt ttatcagact ccaacaatgc ccttggaggt
     2521 cttgggcgcg cttgggatag taataagggt gtttctcata ttcttcaaga agttttttgt
     2581 gctcttctac ttttagctgc atttcatgaa ctgcatcctc atagtgtttg gccaatgctt
     2641 catggtcagc actagttttg gcattacgaa ccgcgctggt catctccatc ggatggggat
     2701 tcatttgtgc acaggaaatc aatgaagcca gaatcaccaa agttgcaaaa aatatacgtg
     2761 ctttcataag tatctcctgg tagttgaatc aaagaaatat acctaaattc tataatttaa
     2821 ttcgtaatcc tcggtataaa gtagccaccg agtatcccca atgctgtgat aattgaaaga
     2881 atacgccgtc cttagcggtt agtgagtgaa ctcgctgttg gtgacaagat caagccctac
     2941 aatatagttc atctgcttga cactaacgaa ggatgattat ggcatttagc aataaccatt
     3001 ttttgcagca cttcctcttc ggcgctgaaa gcatctacgg cttccgggtc aaactggctt
     3061 cccttcatac gtagaatctc tgctttggcg aactcaaaag aatgactctt acgataaggc
     3121 cgattggaag ttatcgaatc caaggcatct attaccatga atagacgcgc acctaatgga
     3181 atacgaccgc cactcagtcc ttttgggtaa cctgaaccat catagcgttc ctcatgacag
     3241 agaatgatat ttgctgcatc tgtcatggca ggtatttgcg aaatgagatg gtagcctttt
     3301 tctggatgtg tccgcatttc gatccattca gattctgtta aagagccagc tttcagtaaa
     3361 atggcatcgg agataccaat cttgccaata tcatgcagta atgttcccca gtatatttgc
     3421 tgcaataaat tcggatcgga ggtaaagcga cgtgcgagca ccatcgtatg gcaggcgacg
     3481 cgcttggagt gcaaacccgt ttcgtgctcc cgcagatcca gcgcattcac caaagtttct
     3541 gctaagaggt tgaacatatc ttcctgactg gcacagacat ctatggtttt gaggaaacaa
     3601 tgttcggaac agtatgtagc acccacagaa atatacggtt gcctttcaag aagctttcca
     3661 caatgttggc aatacgcttc tgggtgcctg cctgttttgt tcatttgatc cccgtctaac
     3721 atgtgtgctc cattagtaca cagagatcaa tataattatt atttttcaaa tctcctttat
     3781 aagacgatag cgaatgaatt ttctcagctc cagaacgatc aagggtatga atcctaccgc
     3841 gaaccatcct atgcattgac ttagcgtaat aggttcaacc ccaaataagg tttgaaacat
     3901 cgggacatgg tgtatcgcca actgcaagcc aaaacatact gctacaacca aaaataacct
     3961 tatattggaa aacatgctta tttcccaaat ggcacgctgc tcgcttcggg ccccaaaagc
     4021 gcgtagcagc ccagcaatca ccaaagtagt aaatgctgca tcgcgagctt gttcaagact
     4081 gctacccatt atatagagtt cataggcaaa tacacccagc gtgacgctgg cggtcagtaa
     4141 gccggtaaat agcgttagct tgattaaatc acgattgatt agtgccgttt gtggatgccg
     4201 cggtggacga tccaatacat cggagtcaac tggatcggtc gacaacgcca gggctgcaaa
     4261 accatcagtt accagattga tccacagaag atgcaagggc aatagaggga gcggccagcc
     4321 aagtagaact gcgaccagca tcacgattaa ttcgccggta ctgctaccaa gcagataagc
     4381 caaagtttta gcaatgttgt cataaatgcc ccgtccttct tccacagcag cgacaataga
     4441 agcaaagttg tcatcggtga taatgatatc ggccgcctcc ttggtcacct cggtgccagt
     4501 tacacccatc gcgataccaa tggatgcttc cttgattgca ggcgcatcgt tcactccgtc
     4561 tccggtcatc gccactaccg cactctggga cttccacgcg cgaacaatgc gaagtttgtg
     4621 ctcggcggtg acgcgcgcat aaacggaaac ccttgataca cgctccttca gcacctcatc
     4681 attcaatcgg tcgagttcga tacctgcgag aatttcgtcg ctcttaccca gaatacccag
     4741 ctcacgccct atcgcacgtg ccgtatcagg atgatcacct gtaatcatca cagttttgat
     4801 gccagcccgc ttgcatttgg ccactgcttc ttttgcctcg gcgcgtggcg gatcctgtaa
     4861 gcctattagc cccagaaaga taagttcctt ctcgatttcg gcatcatctg tcactttgcc
     4921 ttcttcgaaa ctgaagccat ccaggatgcg ctcagcaatt gcaagcacgc gtagcgcatc
     4981 gtttgccagc aacgtattag catggagaag ccggacgcga tcactttctg tcagatccct
     5041 aattccctgg tcggtacgga ttcgggtgca gcggccgaga atcacttccg gtgcaccttt
     5101 ggcgaatgcc caggaattat tttcctgccg acggattaca gtcatacgtt tgcgagcgga
     5161 atcaaaaggg acaatggcca gtcgtggcat ttcagcctca atcatttgcc gggtgatatt
     5221 gcctttggcg gcagcaacca gcagcgctcc ttcagtagga tcgcccacta tcatagatcg
     5281 accatctatt aaagctagct cggcatcgtt gcaagcagcc gatgcacgga gcagcgcgag
     5341 aagtgatggg gttccctcta ccgggatttc tgcatttccg acaaaaaacc ccccctcggt
     5401 tgaataacct tcgccaggga tgcgatacaa gttatctgcc gtgattaatt tgcaagcagt
     5461 catttcaccc attgtcagag tgcccgtctt gtccgtacaa atgacttgag cgcggcctaa
     5521 tgtttcaatc gaagccaggc gccgcaccaa tgcattccgc cgcaccatgc gttgtacacc
     5581 caaggcaagc gcgatagtca ctactgctgg caatccctcg gggatagcag caacggcgag
     5641 actcaccgtg cttaagaaaa gctcaaatgg cgctatcccc cgcaataatc ctagcccgaa
     5701 aatcagtgca acaatgctga aacaggccag caataacata tgtcctaccc ggtcgagctg
     5761 gacttgcaac ggagtttctc cactttcggc agtctccagc agtttagcga tatggccgag
     5821 ttccgtttcc atgccggtat taaccaccaa tgcgcgaccg gaaccgcctg ttacgctggt
     5881 accaagaaaa accatatttt ttcgctcagc caaagctgtt tctggaggca gattatccgt
     5941 gaacttgttt acagcttgcg attcacccgt gagcggtgct tcgttgatgc gtagaacaga
     6001 agcctgaatc aggcgggcat ccgcagtaac cagatcccct ccttctagca gaagaatatc
     6061 gcccggaaca atttcggttg cagcaaccac cacgctatgg ccattgcgca ctacgcgaca
     6121 atgaggcgct accatatcag ccaacgcggc agcagccttt tcggcacgat attcttgaat
     6181 gaaaccaatc agcgcattca atattacgat ggcaataata gcgatgccat ctacagtttc
     6241 acccagcgct gttgaaacag ctgctgcgcc aatcaatacc caaataacca ggcttttaaa
     6301 ctgacttgcc aaaatagcaa aagctgaaaa tcgcttaccc tttcgtagtt tattaggccc
     6361 attccggacg agccgatcaa ccacttcttc atcagtaagt ccccgttcca catcggttgc
     6421 aatgaggtgt tcgagttcgg ctattgattg gctatgccat gcattcatag tttatttctc
     6481 attggcttgc aggcacggta taaagcgatg gctccgacca ctgtgcgata acgggtagtc
     6541 tcctccactg gatggaatcc ggcatctgct ataaaaaccg gcaataaacc cagaatgtta
     6601 tcgtgaacct cttcagccca ccgcattacc agagagatta gccgcatgac tgaatcgtgc
     6661 ggtttgccga agtccgcaat atgcaactca ccaccgggct tcaacacgcg aaatgcttct
     6721 ctgagtgctt gctgtttatc ttcccgcgtc aaatgatgca gcatcaggct ggcgaaaacg
     6781 tgatcaaaac tttcgttcag atagggtaga caagttgccg taccctgttg caggacgata
     6841 ggctctcctg cttgctcggc cttccttcgt gcgatgtcca atatctgagg attcatatca
     6901 agaccatttt ctccggtatc cggttgaatt tgtttgatca tcagagtaag tgtgcccgta
     6961 ccgcaaccca catccagcac attttgacta ggctggatgc gggcttgcgc gatcaatgca
     7021 gtcttaattc ccgattcagg gaataagcgc cgcattatcg gatcgtacca acgcgtcaac
     7081 cagtggaaat gaaacgccgg gatatagcga ggggaattct tcatgatgat aaacctccca
     7141 tcttttcagc tttaagtgaa tgccgaattg agatttcctt gacaagtgca tagattgctg
     7201 gaaatacgat caatgttagt attgtagcgg aaaccatacc gcccaccatc ggggcagaaa
     7261 tgcgcctgat cacttcggaa cctgtgccgc tactaaacat aaccgggagc agacccacaa
     7321 cgctcccagc aatggtcatc attacaggtc gtatccgata taccgcccct tgcactaccg
     7381 cttcgtaaag atcctcgaca gtgactttac taccagcagc ttcgcgcctt gcagtcacat
     7441 ccttgaacgc ctgatcaaga aactcgagca taaccattcc agattcagcc gcaatgccga
     7501 cgaggccgat gaaaccaatc gctgctgcga tgctcatgtt gtagtcgagc caatacatca
     7561 accagatacc cccgactaag gaaaaaggta ccgatagcat tactatcaat gtctctgtga
     7621 gacgattgaa attcagatat acaagcagga ataccatcag aagagtgatt ggcaccacta
     7681 ctttcagttt ttcgctggcc cgctccatgt attcgaattg cccgctccag gtggcatagt
     7741 atccgggtgg aaattgcacc tgttcgcgca ctgctttctg tccggctgta acgtagctgc
     7801 cgatatcacg atcacgaata tcaacaaaaa tatacgcgga cagtagtgca ttctccgtac
     7861 gaatgcttgg cgggcctttg acaaggctga ttcgtgcaag ctggccaagc ggaatcatcg
     7921 tcgctcccat ggttggaacc agcacctgag tagcaattgc ctgcggatcg ctgcgtagct
     7981 cgcgtggata gcgtacggta acaccaaagc gttcccggcc ttcaaccgtg gtcgtcacca
     8041 tctcgccgcc aagcgctgct gaaatcacag acaataaatc gccgacagcc aatccatatc
     8101 gggcaagttc aagacgatcg ggctcaacat caagatagta gccgccggtt gtgcgttccg
     8161 cataggcgct actggttcct ggcactgttt ttacagctgc ctcaatctgc ttggcaagtt
     8221 tttcaatctc gcccaaatcc ttgccgaaca ctttgattcc gacgggtgtg cgtatgccgg
     8281 tggcaagcat atcgttccta tttttgatcg gcatcgtcca ggcattactg actcctggca
     8341 tttgtaatac ttgatccaat tcggcgatca acttgtcgat ggtcatgcct ggacgccatt
     8401 cgctttccgg tttcaaatta atgatggttt cagtcatttc cagcggcgct ggatcagtag
     8461 cggtattagc gcgtccggct tttcccaata ccgatgccac ctcgggaaag cttttaatga
     8521 ttttattctg tgtttgcagg atttctccag ccttggtaac ggaaatacca ggaagtgtca
     8581 ctggcatgta aagcagcgta ccttcattca atgttggcat aaattctgtc cccaacttga
     8641 gcgctggata gacagtaatc ccaactacca cgattgctac catgacaatg acttttttcc
     8701 actgcaacac ccttgccatt acaggccgat agagctggat catgatgcga cccaacggat
     8761 tatcttgctc gcgcggaatg cggccacgaa gcagaagcag gattagagct ggtaccaaag
     8821 tgatcgaaag caacgcagcg ccagccatgg caaacgtttt ggtatatgca agcgggtgaa
     8881 acatgcgccc ttcctgcgct tcgagcacaa acaccggtat aaacgatacc gtgatgatca
     8941 gcaggctgaa aaataacggt gggccgactt ccttgcaggc tgcaatgaca gcctccgtgc
     9001 gcgattcatc gggagcgagt cgcgcaagat gtttatgcgc gttttcgacc atgacgatgg
     9061 cagcatcggt catttctgcg atcgcaagcg caatgcctgc gagactcatg atgttggaat
     9121 tgatgccaag gtgaaacatt ccaatgaagg cgatcagcac accgatcgga agcataatga
     9181 ttgccaccaa cgcactgcgc acatgcatga gaaaaaccac acataccagc gccacgatta
     9241 tgatctgttc gataaatact tcgtttagcg tcgcgatggc gcggtgaatc agctctgagc
     9301 gattgtagac aggctgaatc gaaacacctt ccggtaagcc gggtgcgatt tcgtcgatct
     9361 ttttttcgag attatgggta acattgagtg cattctcacc atgtcgtgct actgcaatac
     9421 ccgagacgac ttctccttct ccgttgaatt cagtgatgcc acggcgctca tcaggaacta
     9481 attccactcg agcaacatga ctcagcaata ccggcatacc ctcgtgtgcc ttcacaacca
     9541 gattctcaag gtcgctaatt ccgcgcagat agcccttacc gcgaaccata tactcggtct
     9601 ccgtcagttc gatcactcgt cctccaacgt cgcgattgct gctggcgata acctcagaaa
     9661 ttgttttgag tgatattccg taagcttgaa ggcgtcgcgg atctacagta acttggtagt
     9721 tttttacaaa tccgcctaca ctcgccactt ccgacacgcc atgtgttgca gtcaactgat
     9781 aacgcaaaaa ccagtcttga atagctcgca attcgtcgag tgcgtgattc ttggcaacta
     9841 gcacatattg gtaaacccaa ccaacccccg ttgcatccgg gccgagcgaa ggcctaacat
     9901 tttctggtaa ccggtttgcc gcgaagctca ggtattccag tactcgcgaa cgcgcccagt
     9961 agatgtcggt tccatcttca aagatgacgt atacaaacga tacaccgaaa tttgatatcc
    10021 ctctgacaac ctttgatttt ggcacactta acatagctgt agtcagcgga taagtaacct
    10081 ggtcttcgac cacctgcggc gcctgtccgg gatattccgt gtagatgatg acctgtacat
    10141 ccgataaatc tggtattgcg tcgataggcg tcttcaatac tgcatacacg ccccaagcca
    10201 ccacaaaaaa ggttccaagt agtaccagaa agacatttcg taccgaccat tcgattatat
    10261 tgccaaccat cttagtgccc tccgtgagcg ttggtgccaa ggctggaatc cgctggctga
    10321 atgtgcacga tggcatactc accggctgat tcctcggcga tttcgaatat aacctgttgc
    10381 ccaagcttca gtgaacgaag taacgctgga tcagaaacgt ggaaatccat tgtcatcgaa
    10441 ggccactgaa gactggtgat cggcccatga gccagtgtga ctgttgcatg ggaaaaatca
    10501 atggcattga tggttccttc accacgatgt gtttttgacg gcgatgtctt aacaaacgag
    10561 ccagttccag tttccttttt ttcatccggc tgttggtggc ctccgtgacc aaagccactc
    10621 agaaccgcct tgagattact ttctgcatca atcaggaaat tagctttaac cacaatggtt
    10681 tctccagcat taattccccc taacacctca gcatagccat cggcatgcat acccagtttg
    10741 accgtacgcg gctcaaatcg gcctgcaccg agatccacca acaccacccg gcgggttcca
    10801 gtatccaaca cagctgagtc gggtattgcc agtaccttgt ctttgctatg agatgaagcg
    10861 aactccacgc gcgcatacat ggcaggtttc agcaaaccat cgggattggg aagtacaaca
    10921 cgtacgatgg cagtacgcgt ctccggtgtt accgtgggat agatgaaagc gatttcaccg
    10981 ttgaatactt tgttcggata agagtccacc ctgattgtgg ccacctgacc ttgatgcacc
    11041 attccaagat cctgttcaaa tacgtcagct agcatccaca cactggataa atcaacaatc
    11101 tgataaagca cctctccagg cataaagcgt tgacccatga ctgccttctt ttccagcacc
    11161 acaccatcgg ctttagagcg caatgtcata tactgccgca cttctccggt ttgctgcagg
    11221 cgttgtaact cggtttccgc aatatcccaa ttgcgtagtt tctgcaatgc gctggcaacc
    11281 aatcgctgca tggaggaccg cacatcggta tcgctatcag cggtggattg caatcctctt
    11341 atcgcaatca agtattcctg ttgcgcggta atcaaatcag gactatatac atccattaat
    11401 gcatcgcctt ttttcactac ctgacccgta gtgtttacat agagccgctg gatccaaccc
    11461 tcaaatttgg tcactaacgt gtatagcttg cgctcatctg cctgtatcgt tgccacagct
    11521 ctaatagtgc gagtcagttc acgtaatgtt gcggtttcag tccgcacccc tagcttttga
    11581 attttttcgg tgcttatttt tacgatggtt tgatccgatg gagattcttc tccctcatag
    11641 accggcagat aatccattcc cattggatct tttttgggta ccggggaggt gtctggtaaa
    11701 cccattggat tgcgataata taaaatcttt ttttcggtgt tgggtgtttc ggataaagca
    11761 acaccgggtg atctttgtga ttgcgtacta ccccaccaat aacttgcaaa cagtgcagtt
    11821 gtaatcgcaa ctccaaccat aattagctta acgacggatt tcataggaat ctccataatc
    11881 ggtaacagtt agtgctgttt cttgccagcc agtaaatcaa tctccgccac tgctttgttg
    11941 taattcgcca tcgcggtaat gagattggcc tcaaaatcaa atactgtcat ctgattatcg
    12001 agcaatgtca gaaaatcaac ccggttcacc tgataagcag ccaatgcgga ttcaaccgtc
    12061 aatttcgctt gtggcaaaat ggcggtttga tagagcttga ccgatttcag gctttgctcg
    12121 gcgatagcga tttgctgacg cagtcttgct gtgatttcat tcgtttgtgc ctgatataaa
    12181 ctgattgtct gatcgcgcaa tgcttgagat tccataatac gcggctctat tttattgcca
    12241 cgccataccg gcagattgac ggctaccgtc atgctaacca tgtcgtcgcg cctcgtacca
    12301 tccatcatgt tgtcacgctg gccatacgaa agacgcacat cgaaatccgg atagtacgcc
    12361 ctgcgcgcta aatcaatgga tttctcatta cgtgcaatca ggctttgcaa tgcaagcaat
    12421 tgtgggcgtt gcgctatcgc ttcttgttga aatgtctcca gattaagcgg cacttcttga
    12481 atttgtagtg gcaccggaat cggaatctca cctttcgtgt tgcgtcccag ggtgcgaatc
    12541 agttccgctt caaacactgg ctgttcgcgc gccaatacta ataacctgtc cagcattctc
    12601 gatacttgtg tttgtgcttt cagtacatcc gcctgactgc cttgtccaac ttggtagcgt
    12661 tcttcagcaa tgcgcaaaaa acgttcgagt gtttgtttat ttttttcagc caatttgatc
    12721 atttccagcg taagcccgag atcaaagtaa gctgttttaa gatcgtgcac aacacgatta
    12781 accgtttcat gatagccctg ctcaatggct tcggcatcct tggcggcaac atctttacgc
    12841 aagccgcgct ttcccggaaa aggcaaacgc tgggatagac cgatcatttt catggtcata
    12901 tcctcgcggt tgaatggtga agatgccaac ggtgcattga ttacgccagc ctcaagcatg
    12961 gggtcatcca gcgcttccgc tggtgatact ctctgctgag cagcttcacg ttcttgatac
    13021 gcagcctgaa tctccggatt attttccagt gcttctgcaa tcaaacttgg gagcagcgtc
    13081 acgttgtggg agttggatgc tgttaaagtt gcctttgtag cagactgtgc aaaaattgct
    13141 gtaccagcct tgtttaaaat atcttcctta ccctgagcat ttgccggaag tacgataaca
    13201 gcaagcactg ccgcaacaat tctgccggga atcactgcga actcccggtg gaattttgct
    13261 tactcaattt tacgatgacg taattggaag cttctttttc aagagagaat tcaacgatat
    13321 cgccttcttt aattttagta gccaatgagc gttccttcag gcgaaagggc atagtcattc
    13381 ttggccaatt tagtgcagcg atcgggtcat gatttatttt tattgtggcg ttggattgat
    13441 cgacctgcag caccac
//
LOCUS       DAOQKQ010000062        13438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_2160_length_13438_cov_7.034297, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000062 DAOQKQ010000000
VERSION     DAOQKQ010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 13438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13438
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2160_length_13438_cov_7.034297"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /gene="ipk"
                     /locus_tag="PKZ49_05605"
     CDS             <1..522
                     /gene="ipk"
                     /locus_tag="PKZ49_05605"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648812.1"
                     /note="An essential enzyme in the nonmevalonate pathway of
                     isopentenyl diphosphate and dimethylallyl diphosphate
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HND36072.1"
                     /translation="TLIMLNSLWGLNWHRDRLMELGLKLGADVPVFIYGDNAFAEGVG
                     EKLSAVNLPNAWYLVLMPPVHVSTAQIFASNELTRNTIPITIPPFSIRDGHNDLEPVV
                     CRMYSDVAKCIDWLKKLDNTTLVAMTGSGSSVFAEFETEAQAKEAYLRLPDGVNAVVA
                     KGLTKHPLKDFYR"
     gene            530..604
                     /locus_tag="PKZ49_05610"
     tRNA            530..604
                     /locus_tag="PKZ49_05610"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:562..564,aa:Gln,seq:ttg)
     gene            732..1682
                     /locus_tag="PKZ49_05615"
     CDS             732..1682
                     /locus_tag="PKZ49_05615"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HND36073.1"
                     /translation="MSYDSLMVFTGTAHPKLAQDVVKYLNIHLGRATVSRFSDGEIMV
                     EILENVRGKDVFVLQSTCVPTNDSLMEILVIVDALKRASAGRITAAIPYFGYARQDRR
                     PRSARVAITAKVVANMLTTVGIDRLLTMDLHSDQIQGFFDIPVDNIYGMPILLGDVWK
                     HNYQNLIVVSPDVGGVVRARHMAKRLECDLAIIDKRRPKPNESKVMNIIGEVKGRTCV
                     IIDDMVDTANTLCEAARALKDEGAQSVVAYSTHAVLSGKAVERIASSQLDKIVVTDTI
                     PLSDEAKACDRICQLSIANLLGETMLRISNESSLSSLFME"
     gene            1752..2366
                     /locus_tag="PKZ49_05620"
     CDS             1752..2366
                     /locus_tag="PKZ49_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HND36074.1"
                     /translation="MQIEIDANNRAMQGKGASRRLRRDGKVPGIVYGGEGQAQSIELD
                     HNNLFHKLKLEAFHASILSLNLDGKKEQVLLRDVQMHPYKQQVLHVDFQRVDQNRKIH
                     MKVPLHFINAEIAPGVKLSGGLISHVLTEVDISCLPKDLPEFITADLSEMTAGSTLHL
                     KDLKLPNGVEIIALSKGDDLPVATLTVKRAEEGAEEGSSATPAA"
     gene            2523..3092
                     /gene="pth"
                     /locus_tag="PKZ49_05625"
     CDS             2523..3092
                     /gene="pth"
                     /locus_tag="PKZ49_05625"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HND36075.1"
                     /translation="MKLIVGLGNPGEKYALTRHNIGFIWLQQLAAELKVSFVSEAKFH
                     GLCASGIYQGEPFRLLMPQTYMNASGMAVASLCRFYQCNPEQILVIHDELDLPPGTIK
                     LKLGGGSGGHNGLKDIITRLGDKDFWRLRIGIGHPGDRAAVVDYVLRPPSKQEAVLID
                     ESISRSLTLCQLLLSGDYMAAMQKLHSSQ"
     gene            3162..4253
                     /gene="ychF"
                     /locus_tag="PKZ49_05630"
     CDS             3162..4253
                     /gene="ychF"
                     /locus_tag="PKZ49_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HND36076.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTKAGIAAENYPFCTIDPNVGIV
                     EVPDPRLIKLSEIVKPKVVQSATVEFVDIAGLVAGASRGEGLGNKFLANIRETDGIVH
                     MVRCFANDNIVHVAGRIDPISDIEVIQTELALADLTTVEKAVQRESKLAKSGNKESIR
                     NVTVLEGLHNHLDQNKPARSFKFEDAQHALIKSLCLLTMKPTIYVTNVADTGFQDNPL
                     VKSVQDYAAQEGAPVVVICASLEAEIADLSDDDKQVFLADLNLTEPGLNRLIRAAYQL
                     LGLQTYFTAGVKEVRAWTFHSGDTAPQAAGVIHSDFERGFIRAEVIAYDDYVTYNGEQ
                     GAKEAGKMRLEGKDYIVQDGDVVHFRFNV"
     gene            complement(4323..5564)
                     /locus_tag="PKZ49_05635"
     CDS             complement(4323..5564)
                     /locus_tag="PKZ49_05635"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HND36077.1"
                     /translation="MRILFIHQNFPAQFKYLAPTLAKQHQVRILTLNARLADNWQGIP
                     VNHYALQKASTKGIHPWIIDFEAQVIRGEATLYAAFNLRQQGFTPDVIIAHPGWGESL
                     ALKDVWPDAKLGLYCEFFYRAEGADVGFDPEFPNTDPALASRIRLKNTNTLIQLSTFD
                     TGISPTDWQASTFPELFRNRISVIHDGIDSTLLQPNPSATLTLKINSGDLLTLSREDE
                     IITFVNRNLEPYRGYHTFMRCLPRLLKQRPQARVLLVGGDGTSYGAKPLGSKSWKERF
                     IDEVHPQISEADWSRVHFMGNLPYQHFVTLLQCSSLHVYLTYPFVLSWSLLEAMSIGC
                     AIVASDTSPVRDAIIHNETGRLVNFFDYKALADEISSLLDNPDERQRLGANARALIYE
                     KYDLKTICLPKQMEWVENILK"
     gene            complement(5725..6774)
                     /locus_tag="PKZ49_05640"
     CDS             complement(5725..6774)
                     /locus_tag="PKZ49_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36078.1"
                     /translation="MAINHEQINFDQSSFNQIKQSLQQAIKDKANSLRDNHAVTGVSS
                     FKEVLSKTVQDLLSSPSEKAIKNGSSQNTHALTLDRPIASSRDVQPIQKLISSAADYL
                     STRLQNEEKHTTELATEKALNNTAVSSFLPINAGQNIAQKISSEESTHESNTGLSSAT
                     WSGGKPDSIKELLDFMHTRGIAMNVGEASDFLYGSVGANDDLRDFNTILNAESPTTAN
                     NQALGQLFSNPSARINPNYATRSAEEVVAQHGNLLVRESKNGAQILAAQAANGMPLTD
                     ISNSATGVGGGIARYGITDANVQAILDNPKVSDDIKAQLKSYLGSGYQQNPTNYLLDI
                     NLASFATTGVFNQSK"
     gene            complement(6956..9511)
                     /gene="glgP"
                     /locus_tag="PKZ49_05645"
     CDS             complement(6956..9511)
                     /gene="glgP"
                     /locus_tag="PKZ49_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HND36079.1"
                     /translation="MINKTVFTITINPKIPPRLSRLEELANNLWYSWDRSTRTLFSRI
                     DSRLWEAVGHNPKSFLKRVDESLLHKAASDQTFLASYNSVLSAYDSYHSEPARYHRFE
                     GLESRDQIAYFCFEFGFHESLPVYSGGLGILAGDHCKAASDLCLPFVAVGLLYRQGYF
                     SQTIDSQGNQRAIYNDSDFGDLPVEPALHDDGAEVHINIDLPGRSVNVKVWRVIIGHV
                     SLFLLDTDLPDNSAADRLITHQLYGGDKIMRIQQEILLGIGGVRALQAIGVKPTVWHS
                     NEGHAAFMMLERARNLVQHGLDFASALEAVATNTVFTTHTAVPAGHDHFPQTMMQEYF
                     GLFCNDLKIAWSEFMALGNMPGNEDFNMTALAIRLSRTHNGVSKIHGDVSASICRDLW
                     PQIEPEENPISYITNGVHVPTFLAQEWSVLFDRYLGHEWRNKMCEPDFWSQIDAIPDH
                     LFWSVRQTLKSQLFYGIRARLTEQNARNRGSEAHLDRLFKWIDPNNPNILTLGFARRF
                     ATYKRATLLFENLDWLRKILLDQDRPVVLIYAGKAHPADIPGQDLIRRISHVSRMPEF
                     EGRILLVEGYDLRLARRLVAGVDVWLNSPIFPLEASGTSGMKAGINGTINLSVLDGWW
                     GEGYDGKNGWAVKPGSENTEAVLRDQDESQSFYEILQDQVVPLYYRRNGFGYPAEWVK
                     MAKYSMASLLPRYSANRMVSEYVEKFYIPATNKGAQFVNDDYAIARNVAVWKAKIRKA
                     WDQVSLRRLDAPAKQINFGDLLNFKVAVKLNGLQPEDVMVELLLCRQYKKMRLTNFKR
                     FKFDCVGMVSPQEHLFELNHAPELCGRQEYYLRIYPYHSALTHPLEMGMMIWL"
     gene            complement(9537..10085)
                     /gene="orn"
                     /locus_tag="PKZ49_05650"
     CDS             complement(9537..10085)
                     /gene="orn"
                     /locus_tag="PKZ49_05650"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HND36080.1"
                     /translation="MAQDSNNLIWIDMEMTGLNPDSDRIIEIALVITDSQLNTIAESP
                     VLAIAQPISILDSMDKWNRSTHAKSGLIDKVKASTLSEVEAEQIMLKFLQPHVPSAIS
                     PMCGNSICQDRRFLVRSMPKLEAYFHYRNLDVSTLKELAKRWKPTIMSGLNKESKHEA
                     LSDIYDSINELKYYREHFINIG"
     gene            10191..11441
                     /locus_tag="PKZ49_05655"
     CDS             10191..11441
                     /locus_tag="PKZ49_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HND36081.1"
                     /translation="MQTFTAIFVTALLLTTLIELWLAIRHARHVWSNRNQVPNDFATQ
                     IDLASHQKAADYTCAKTRFNYPTIIMHSLLLLIFTLGGGLNVISNFWAGWIENPIFHG
                     MALILTTIFILSAIEIPFNYYRTFVIEAQFGFNKMSPAMFFSDLIKQYSLGLILGGLL
                     LFCMLWLMEKAGAYWWLYAWMAWMGFNVLLLSIYPTWIAPIFNKFAPLEDNALKTRIE
                     QLMQKCGFKSSGLFVMDGSRRSSHGNAYFTGFGKNKRIVFFDTLLSRLEGPEIEAVLA
                     HELGHFKLHHVLKRIVWSFIVSLVFFALLGFLMQQAWFYEGLGVSANTPSTAAMALLL
                     FFLIMPVFTFLLHPLSSLYSRKHEFEADAYAAKHASANDLIQALVKLYQDNAATLTPD
                     PLHSAFYDSHPPAATRIMQLKKLA"
     gene            11459..11797
                     /locus_tag="PKZ49_05660"
     CDS             11459..11797
                     /locus_tag="PKZ49_05660"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HND36082.1"
                     /translation="MTDACDLSNKHCKPCEGGVPPLSQREIDSLLQQLEQWEYRNNTI
                     HKDFTFKNYYQTMAFVNAVAWLSHREDHHPDMVVGYNHCQIVYMTHAIQGLSENDFIC
                     AAKIDGLFKI"
     gene            11794..12732
                     /gene="rsgA"
                     /locus_tag="PKZ49_05665"
     CDS             11794..12732
                     /gene="rsgA"
                     /locus_tag="PKZ49_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HND36083.1"
                     /translation="MSDKRSSPARNSPTRIEGQVIATFGRQYEIKTGAGLILTCFMRG
                     KKGGIACGDQVEILPTTPGQGVIENVLPRVNLFYRSDAFRQKLIAANVTQLIIVVATI
                     PTFSEALIDHYLVAAENEKIQVLIVLNKCDLSETVLAATEKLALYQQLGYPCLSISAV
                     NDIKPLMPYLHQQKSLLIGQSGMGKSTIINALIPEAQRATKEISLALDSGRHTTTHAR
                     LYAIDANSTIIDSPGMQEFGISHIDKANLARGFIEFRPYLNGSCKFSNCRHLTEPGCA
                     LLLAATKGEINARRLAVYQKMMEEAERRKDWLVRSS"
     gene            complement(12715..>13438)
                     /locus_tag="PKZ49_05670"
     CDS             complement(12715..>13438)
                     /locus_tag="PKZ49_05670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HND36084.1"
                     /translation="ISGIVNGIDTIEWDPSADIHIAKKYTIRRLSNKQANKLAIQQKL
                     GLEPRSDQLLFAAISRFSYQKGYDLLFEVAPKLLELPAQLAVLGAGDASIERNLVQLA
                     EQFPGRMAIHVGYEEALSHQLEAGADSFLMPSRFEPCGLNQMYSQRYGTVPLVHATGG
                     LIDTVVDYSPEALKNGTASGFQFHDLSPDAFFEGINRVVAAYQDKDIWEQLQKNGMHK
                     DFSWKTSAATYREIYQQLARAH"
BASE COUNT         3901 a         2880 c         2805 g         3852 t
ORIGIN      
        1 acgttaatca tgcttaattc tttatggggt ttaaattggc acagggatcg tttaatggag
       61 cttgggttaa aattaggcgc tgacgttcca gtttttattt atggtgataa tgcatttgct
      121 gaaggtgtag gagagaaact ttccgcagtt aatctcccta atgcttggta tttagtcctg
      181 atgcccccag ttcacgtctc aaccgctcag atttttgcaa gtaatgaatt gacgcgcaac
      241 acaattccga tcacaatacc gcccttttcc atccgtgatg gacataatga tcttgaacca
      301 gtagtttgcc gtatgtactc tgatgtggca aaatgtatag attggttaaa aaaattagat
      361 aatacaactt tggtagcaat gactggatct ggatcttctg tatttgcgga atttgagacg
      421 gaagcacaag ctaaagaagc ctatcttcgc ttaccagacg gggtaaatgc tgtggtcgca
      481 aaaggattaa ctaaacatcc gttaaaggat ttttatcgtt aatgataatt ggggagtcgc
      541 caagtggtaa ggcaccggat tttgattccg gcattcgtag gttcgatccc tacctcccca
      601 gccaactacg gttcattgct tagtattagc tttatttatt tgcatatagt tttatcttta
      661 acctgataat tgttttcaaa gaaagcttgt ttactcccat ctgttttccg ttgatataaa
      721 aataataaat tgtgtcttat gatagcttaa tggtttttac gggtacggct catcctaaat
      781 tggctcagga tgtagtgaag taccttaata tccatctagg ccgagctaca gtatctcggt
      841 ttagtgatgg tgaaatcatg gtggaaatac ttgagaatgt tcgaggtaag gatgtattcg
      901 tccttcaatc aacttgtgta ccaacaaacg atagcctaat ggaaattttg gtaatcgttg
      961 atgccctgaa acgagcctct gcgggacgta taaccgccgc aataccctat tttggttatg
     1021 cacggcaaga tcggcgtcca cggtcagcgc gcgttgccat tacagcaaaa gtagttgcta
     1081 atatgttaac cactgtaggt atcgatcgat tactgacgat ggacttacac tcagatcaaa
     1141 tccaaggctt tttcgatatc ccagtggata atatctacgg aatgcccata ttgcttgggg
     1201 atgtttggaa gcataattat cagaatttaa ttgttgtatc gcctgatgtt ggtggtgtcg
     1261 taagggcaag gcacatggca aagcggctcg agtgcgatct ggcgattatt gataaaagac
     1321 gccctaaacc caatgaatca aaagtaatga acattatcgg tgaagttaaa gggcgaacct
     1381 gcgtcattat cgatgatatg gtagatacag ccaatacgct gtgtgaagct gctagggctt
     1441 tgaaggatga aggtgctcag agcgtcgttg cttattcaac gcatgcagtt ttatcaggaa
     1501 aagcagtaga acgtattgca agttcgcaac tcgataaaat tgttgtaacc gacacaatac
     1561 cactttctga tgaggccaaa gcctgtgatc gcatttgtca gctaagtatc gccaaccttt
     1621 tgggtgaaac aatgctaaga attagtaatg agagctcttt gagctctttg tttatggagt
     1681 aggtaagtag ttttattagt gatttttctg gtcgcggata aaatctgttt agtaatttta
     1741 ggagaaaaag gatgcaaatt gaaattgatg caaataatcg ggcaatgcaa ggtaagggtg
     1801 cgagccgccg cctgcgcaga gatggtaaag taccgggtat tgtttatggt ggagaagggc
     1861 aagctcagtc aatcgaatta gatcacaaca atctctttca taaactcaag ttagaagcgt
     1921 ttcacgcttc gattctttcc ttaaatctag atggaaaaaa ggagcaggtt ctattaaggg
     1981 acgtgcaaat gcatccttac aaacagcagg tcttgcatgt ggattttcaa cgcgttgatc
     2041 aaaatagaaa aatacatatg aaggttcctt tacattttat caatgctgaa attgctcctg
     2101 gagtcaagct atcgggcggt ttaattagcc atgtgttaac ggaagttgat atttcctgcc
     2161 taccaaaaga tctacctgag tttatcacgg ctgatttatc agaaatgact gcaggaagta
     2221 ctttacacct taaagatctc aaactgccta acggggttga aatcattgca ttaagtaaag
     2281 gcgatgattt accagttgct acactgaccg ttaaacgagc tgaagaagga gctgaagaag
     2341 gaagttcagc gaccccagct gcctagcaac gacaagcact agcgcaagga acgatttcta
     2401 cctttacaag gttggcttcg ttccttgcac tatcaatgaa tcgattttct attcgatggc
     2461 aagcaacaag gttttgtact tttcagtaat aaaccctgcc tactgatttt gtttaataac
     2521 caatgaagct aattgtaggt ttaggaaacc caggtgaaaa atatgctctg actcgccaca
     2581 atataggctt tatatggtta cagcagctag cagctgaact aaaggtttca ttcgtatcag
     2641 aagcaaaatt tcatggctta tgtgcaagtg gaatctacca aggagaacct ttccgattac
     2701 tgatgccaca aacctacatg aatgcaagtg gaatggcggt tgcctcgtta tgtcgttttt
     2761 accaatgtaa ccctgagcaa atactggtca ttcacgatga gctggattta ccgccaggta
     2821 caatcaagct aaagcttggc ggtggatcgg gaggacataa tgggttaaaa gatattatta
     2881 ctcggctagg tgataaagat ttttggcgat tacgaatagg gattggtcat ccaggggata
     2941 gagctgcggt agttgattat gtgctacgcc ctcccagtaa gcaagaagca gttctcatcg
     3001 atgaaagcat aagcaggagc ctaaccttat gccagctatt actcagcggt gattatatgg
     3061 ctgctatgca aaaattgcat agcagccaat aatattaatg attgcaagct tatacccaga
     3121 cactaaatag caattttttc ttctaacaat tacgcctaaa tatgagccta aaatgcggta
     3181 ttgtcggtct acctaatgta ggtaaatcta cacttttcaa tgccttaacg aaagcgggta
     3241 ttgcagctga aaattatcct ttctgtacca ttgaccccaa tgtcggcatt gttgaggtgc
     3301 cagatccacg gttaataaaa ctcagtgaga ttgttaagcc taaagttgta caatctgcaa
     3361 cagttgagtt tgttgatatc gctggattag ttgcaggcgc ttctcgtgga gaaggactag
     3421 gaaacaaatt tcttgcgaat atccgtgaaa ccgatggtat cgttcatatg gtacgctgct
     3481 ttgctaacga taatatcgtt catgtggctg gtagaatcga tccaatctcc gatattgaag
     3541 tgattcaaac tgaacttgcc ttggctgatc ttacaaccgt tgaaaaagcc gtacaacgag
     3601 aatcgaaatt ggctaaatcc ggtaataaag aaagcattcg taacgtaacc gttctagaag
     3661 gcctgcataa tcaccttgac caaaataaac cagcgcgcag ctttaaattc gaagacgcac
     3721 agcatgcact gataaaatca ttgtgcttat taacaatgaa accaacgatt tacgttacaa
     3781 atgttgctga tacaggcttt caagataatc ctttggtaaa aagcgtccaa gactatgcag
     3841 ctcaagaagg cgcaccggtt gtagttattt gtgcatctct ggaagctgaa attgcggatt
     3901 tatcagatga cgacaagcag gtattcttag ccgatctcaa ccttaccgaa ccgggactaa
     3961 atcgcctcat tcgggccgct tatcagctct taggactcca aacttatttt actgcaggcg
     4021 ttaaggaagt acgtgcttgg acctttcata gcggtgatac ggcaccccaa gcagcgggcg
     4081 tcattcattc agattttgag cgtggcttca ttcgagcaga agtgattgcc tacgatgatt
     4141 atgtgaccta caacggcgaa cagggtgcga aagaagcagg aaaaatgcgc ttggaaggta
     4201 aagactatat tgtgcaagat ggtgatgttg ttcatttccg ctttaatgta taacaaaggc
     4261 aaagctggac tttttatttt taacttagaa gctcactccc ctcttacctg atttagggaa
     4321 gcttatttca agatattttc aacccactcc atctgtttcg ggaggcagat tgtttttaga
     4381 tcatattttt cgtaaataag tgctctcgca ttagctccaa ggcgttgcct ttcatcagga
     4441 ttatcaagta atgaactgat ttcatccgca agcgctttat agtcaaaaaa attgaccaat
     4501 cgacccgttt cattatgaat aattgcatca cgtacaggag aagtatcgct tgcaacgatt
     4561 gcacaaccaa tactcatcgc ttcgagtaaa ctccatgaaa gtacaaaagg ataggttaaa
     4621 taaacgtgca gcgaggaaca ttgaagtaaa gtaacaaaat gctggtaggg taagttaccc
     4681 ataaaatgca ctcgggacca gtcggcctca ctaatttgtg ggtgtacctc atcaatgaat
     4741 cgttctttcc agctcttcga tcctaatggt tttgcgccat aactggtgcc atcgccaccc
     4801 acgagtaaaa cccttgcctg tggacgctgt ttgagcaatc ttggtaaaca tcgcataaat
     4861 gtatggtagc ctcgatacgg ctccaagttg cgattcacaa aagtaatgat ttcatcttct
     4921 cttgacaagg taagtaaatc acccgaattt atcttcagtg taagcgtggc actgggattg
     4981 ggttgcaaaa gggtcgaatc gattccatca tggataacac taattctatt ccgaaacaac
     5041 tcaggaaaag tgctcgcctg ccaatcagtt ggtgaaatcc cagtatcgaa agtgctcaac
     5101 tgaataagtg tattggtatt ttttagccgt attcgactag caagcgcagg atcagtatta
     5161 ggaaactcgg ggtcaaaacc aacatcagca ccttccgcac gatagaagaa ctcacaatac
     5221 agccctagtt ttgcgtcagg ccaaacatct ttaagcgcta aactttctcc ccatcccgga
     5281 tgggcaatga tgacatcagg tgtaaaacct tgttgccgta aattaaacgc tgcatataat
     5341 gtcgcttcac ctctaatcac ttgtgcctca aaatcgatga tccagggatg aatacctttt
     5401 gtagatgctt tttgtaacgc gtagtggtta accggaatcc cctgccaatt atctgccaga
     5461 cgagcgttta aggttagtat tctgacctga tgttgcttag caagggtcgg ggcaagatac
     5521 ttaaattgtg caggaaaatt ttgatgtatg aataaaatac gcattattgc cggatctgcg
     5581 gcaataattg ccgcttcgga tccaaattat tctacttcta agctatttta tctttaattt
     5641 ctttctatct ttgagcattg caactatcct agccaattat actttcgcta agacagttgc
     5701 actaccaata atacactgac aaagctattt actttgattg aatacccctg tagtagcaaa
     5761 tgaagccaaa ttgatatcaa gcaaataatt cgttggattt tgctgatatc cactacccaa
     5821 gtaggattta agctgtgcct taatgtcatc agaaacctta gggttatcta atattgcttg
     5881 cacattcgca tcagtgatgc catatcgagc aataccacct cctactcctg ttgcactatt
     5941 agaaatatcg gtgagtggca tgccattggc tgcctgagcc gcaagaatct gtgctccatt
     6001 tttactttcc cttactaaca aattgccatg ctgggcaacc acttcttcag cactacgagt
     6061 agcgtaattc ggattgattc ttgcagaggg gtttgaaaac aactgcccca atgcttgatt
     6121 gtttgcagta gttgggctct ctgcattcag aatcgtatta aaatcacgaa gatcatcatt
     6181 ggcaccaaca ctgccataca agaaatcaga agcttcgccc acattcattg ctataccacg
     6241 ggtatgcatg aaatccaaca gctccttaat actatccggc tttccaccag accacgtagc
     6301 cgagctcaat cctgtatttg attcatgtgt ggattcttca gatgagattt tttgagcgat
     6361 attctgaccc gcattaatgg gtaagaaact actcaccgct gtattattta gtgctttttc
     6421 tgtcgctaat tccgtcgtat gcttttcctc gttttgtaaa cgcgttgaga ggtaatccgc
     6481 tgcggaactg attaatttct ggataggttg aacgtctctc gaagatgcga tggggcgatc
     6541 gagcgttaac gcatgggtat tttgtgatga tccgttttta attgctttct cactggggct
     6601 agatagaaga tcctgcacag tttttgacaa aacttctttg aatgaggaaa ctccagttac
     6661 tgcatgatta tcccgcaaag aattggcttt gtctttaatt gcttgctgca agctttgttt
     6721 aatctgatta aatgacgatt gatcgaaatt tatttgctca tgatttatag ccatatatcc
     6781 tcctagttca actgatccta ttaatgttgt cagttaatac tacgcaaatt atgtgccact
     6841 ctaaaataaa aaatgagcaa atcacataaa atgatctcaa atttagacca atccatgcag
     6901 atttatttta atagtactag actagcaaat atgatctgta gcctagcact atattttata
     6961 accagatcat catacccatt tcaagcggat gagttaatgc tgagtgataa ggatagattc
     7021 ggagataata ttcttgcctc ccacaaagtt caggtgcatg atttaactca aacaaatgct
     7081 cctgtggcga aaccattcca acacaatcaa atttgaaacg tttgaaattg gtcaaacgca
     7141 tttttttata ctgacgacaa agtaacagtt caaccattac atcttctggc tgtaaaccat
     7201 ttagcttaac cgcaacctta aaattcaaaa ggtcaccaaa gttgatctgc ttcgcagggg
     7261 catccagccg tctcaatgaa acttggtccc aggctttcct aatctttgct ttccagactg
     7321 caacatttcg agcaattgca taatcatcat ttacaaactg cgcgccctta ttggttgcag
     7381 gaatatagaa tttctctaca tattcgctga ccatgcgatt ggcgctataa cggggtaata
     7441 gcgaagccat ggaatatttc gccatcttga cccattctgc aggatagcca aaaccattac
     7501 gacgataata gagtggaaca acttgatctt gtaaaatctc ataaaaagac tgactctcat
     7561 cctgatctcg taatactgct tctgtatttt cagatccagg cttaactgcc cagccattct
     7621 tcccgtcata cccttctccc caccagccat ctagcacact caaattaatt gtgccattga
     7681 taccggcttt catacccgaa gtaccacttg cttccagcgg aaaaatagga ctattcaacc
     7741 aaacatcgac accagccacg agtcggcgag ccaatcgtaa atcatagccc tccactaata
     7801 aaattcgccc ttcgaactca ggcatgcgtg acacatggct aatccgacgg atcaaatctt
     7861 gaccaggaat atcagcagga tgcgctttac cagcataaat cagcacgacc gggcgatctt
     7921 gatcgagcaa aattttgcga agccaatcga gattctcaaa taacagagtt gcacgcttat
     7981 aggtagcaaa tcggcgagca aatcctaacg tcaaaatatt gggattattg gggtcaatcc
     8041 atttaaagag tcgatcgaga tgcgcttcgg atccacgatt tcgagcgttc tgttccgtca
     8101 atcgggctcg aattccataa aaaagctgtg atttgagtgt ttgcctaaca ctccaaaata
     8161 gatgatcagg gatagcatcg atctgcgacc agaaatctgg ttcacacatt ttatttcgcc
     8221 attcatggcc cagatagcgg tcgaataaga cagaccattc ctgagccaag aaagtaggta
     8281 catgaacgcc attggtgata tatgaaattg gattttcttc tggttcgatt tgcggccaaa
     8341 gatcgcgaca tatactggca gaaacatccc catgaatctt gctaacaccg ttatgagttc
     8401 gcgataagcg aatcgcgagt gcggtcatgt tgaagtcttc atttccgggc atattaccca
     8461 acgccatgaa ctcactccac gcaattttta gatcattaca aaatagacca aaatattctt
     8521 gcatcatggt ttggggaaag tggtcatgtc cagccggaac agcggtgtga gtagtaaaaa
     8581 ccgtattggt cgctactgct tcgagggcgc ttgcaaaatc gagaccatgt tgaactagat
     8641 tacgggcacg ttctagcatc atgaatgcag catgaccttc attgctgtgc catacagttg
     8701 gtttgacccc gatagcctgc aaagctcgaa cacccccaat ccccagtaga atttcttgct
     8761 ggatacgcat gattttatcg ccaccataca attgatgcgt gataagccgg tcagcagcag
     8821 agttatctgg caagtcagta tcaagaagaa ataagctgac atggccaata atgacacgcc
     8881 aaactttgac attaacgctt cggccaggta aatcaatgtt aatgtgcact tcagctccat
     8941 catcatgaag cgcgggttct acaggtaaat caccaaagtc ggaatcatta taaattgcac
     9001 gttgattacc ttggctatca atcgtctggg aaaaataacc ttgtcgatac agcaacccta
     9061 cggcaacaaa cggtaagcat aaatcactag ctgctttaca atgatcaccc gctagaatac
     9121 ctaatccacc tgaataaacc ggtaagcttt catggaaacc aaattcaaag cagaaatagg
     9181 caatctgatc gcgcgactca agtccttcaa atcgatgata tcgagccggc tctgaatgat
     9241 aggaatcata agctgacaat acactgttat agctagccaa aaaagtttga tcacttgctg
     9301 ctttatgaag tagcgattca tcaacacgtt taagaaatga cttgggatta tgtccaaccg
     9361 cttcccataa tcgagaatca atgcgagaaa acaatgtacg cgtacttcga tcccaactat
     9421 accagagatt atttgctaac tcttccaagc gcgacagacg tggaggtatc ttagggttaa
     9481 ttgttattgt aaacactgtt ttattaatca ttccgtactc cttcattgaa agctgatcat
     9541 ccgatattga tgaaatgctc acgatagtat ttcaactcgt tgattgaatc ataaatatcc
     9601 gatagggctt catgcttact ttctttattt agaccagaca taattgtggg tttccagcgc
     9661 ttggctagct ctttgagcgt actaacatcg agattccgat aatggaaata agcttcgagt
     9721 ttaggcatgg aacgaaccaa gaatcgccta tcctggcaaa tcgaattacc gcacatcggc
     9781 gatattgcag aaggcacatg aggttgcaag aatttcaaca taatttgttc tgcttcgact
     9841 tcagagagcg ttgatgcttt aaccttatca atcagcccag atttggcatg agtagaacga
     9901 ttccatttat ccatactatc gagaattgaa atgggttgag caatagccaa aacgggagac
     9961 tcagcaatgg tatttaattg tgaatctgta ataactaaag cgatttcgat aatgcgatcg
    10021 ctatcaggat tcaatcctgt catttccata tcgatccaga tgaggttgtt gctatcctgt
    10081 gccataatga gccttcgtga attatattaa gcggattgtg tcatattgcg agttacacgt
    10141 cacttttgcg taaatgggcg tgtgtgatta atctttttta tttatccact atgcaaactt
    10201 ttactgctat ttttgttact gcattattac ttacgacact cattgaacta tggctggcga
    10261 ttcgtcatgc acgtcatgtt tggtcaaacc gcaaccaagt cccgaatgat tttgctacac
    10321 aaattgattt ggcttctcat cagaaagcag ccgattatac ctgcgctaaa acgcgcttta
    10381 actatccaac cataatcatg cattcacttc tactgcttat atttacgctg ggaggagggt
    10441 taaatgtaat ttccaacttt tgggctggat ggatagagaa tcccattttc cacggcatgg
    10501 cactgatctt aacaacgata tttatactca gtgcgatcga gattcctttt aattactacc
    10561 gaacctttgt aatcgaggca cagtttggct ttaataaaat gagccctgcc atgttttttt
    10621 ctgatttaat caaacaatat tcactgggac tcattctggg tggtttattg ttattttgca
    10681 tgttgtggtt aatggagaaa gcaggagcgt attggtggtt atacgcttgg atggcttgga
    10741 tgggctttaa tgtcttgcta ctctcaattt atccaacctg gatcgctccg atttttaaca
    10801 agtttgcacc acttgaagat aatgcactta aaacacggat tgagcagctc atgcaaaaat
    10861 gtggtttcaa atccagcgga ttgtttgtaa tggatggatc acgccgtagc agtcacggaa
    10921 acgcctattt cactgggttt ggcaagaata aacgcatcgt ctttttcgat acgctgctgt
    10981 ctcgcttaga agggcctgaa attgaggccg ttcttgcgca tgaattaggt catttcaaac
    11041 ttcaccatgt actaaagcgc attgtatggt cattcatagt aagccttgtc ttttttgcgc
    11101 tcctcggctt tttaatgcaa caagcgtggt tttatgaggg gctaggtgta tctgccaaca
    11161 ctccttccac ggctgcgatg gcattattac tctttttctt aatcatgccc gtgttcacct
    11221 tcttactgca tcctttatcg agtctttact cacgcaagca tgaatttgaa gcggatgcct
    11281 atgcagcaaa acatgcttca gcaaatgatt taattcaagc tctcgttaag ctttatcaag
    11341 ataatgcggc aacgctgaca ccggatcccc tgcattctgc gttttacgat tcgcatcccc
    11401 ctgcagcaac tcggattatg cagctaaaaa aacttgctta accaacaagg agaataccat
    11461 gactgatgcg tgtgatcttt ccaataaaca ctgtaaaccg tgtgaaggag gggtacctcc
    11521 cttatcgcag cgagaaatcg atagcctctt gcaacaactt gaacaatggg aatatcgaaa
    11581 caatacgatt cataaagact ttacatttaa aaactactac caaactatgg catttgtaaa
    11641 tgcggttgcc tggttatctc atcgggaaga tcaccatccc gatatggtgg taggctataa
    11701 ccactgccag atcgtataca tgacgcatgc aatacaaggt ctatcagaaa atgattttat
    11761 ttgtgcagcg aaaatcgatg gactctttaa aatttgagcg ataaacgatc atctcctgct
    11821 cgtaacagcc caacacgcat agaaggccaa gtgatagcaa cctttggccg ccaatatgaa
    11881 attaaaacag gcgcagggtt aattctgact tgttttatga gaggaaaaaa gggagggatt
    11941 gcctgtggtg accaggttga gatcctccca accacgcccg gccaaggtgt catagaaaac
    12001 gtattgccaa gggtaaattt attctaccgg agcgatgctt ttcgtcagaa attaattgca
    12061 gccaatgtca cccaacttat tattgtcgtt gcaacgatcc cgaccttcag tgaagccctg
    12121 atcgatcatt atctggttgc cgcagaaaac gaaaaaattc aagttctcat cgtactgaac
    12181 aagtgtgatt tatcggaaac agtcctcgca gcaaccgaga aattagcgtt ataccagcag
    12241 ttaggctatc cctgcctgag tataagcgcg gtcaatgaca tcaaaccact catgccgtat
    12301 ttgcatcagc aaaaaagcct actcatcggc caatccggga tgggtaagtc aaccattatc
    12361 aatgcactca tacccgaggc gcaacgtgca accaaagaga tttcattagc acttgacagt
    12421 gggcgccata ctacgacgca cgctagactc tatgcaattg atgcaaacag caccatcatt
    12481 gattcacctg gaatgcaaga attcggaatt agtcacattg ataaagctaa tttggctcgg
    12541 ggatttattg aattccgccc ttatctgaac gggtcatgca aatttagtaa ttgtcgccat
    12601 ttgaccgagc ccggttgtgc tttgttgctc gcggcaacaa aaggtgaaat aaacgctcga
    12661 cgtttagcgg tttatcagaa aatgatggag gaggcagaac gccgcaaaga ttggctagtg
    12721 cgctcgagct aactgttgat aaatttcgcg ataagtagcg gcactggttt tccagctgaa
    12781 atctttgtgc atcccattct tctgcaattg ttcccatata tctttatctt gataagccgc
    12841 gacgacgcgg tttattcctt caaaaaaagc atccggtgat aaatcgtgaa actggaaacc
    12901 acttgcagtg ccattcttta atgcctcggg cgagtaatcc accacggtat caatcaagcc
    12961 acccgttgca tgcacgagcg gaaccgtccc atagcgctga ctgtacattt gattgagtcc
    13021 acagggttca aagcgagagg gcattagaaa gctgtcggca cccgcttcca attggtgcga
    13081 cagggcctct tcgtaaccca cgtgaatagc catcctgcct ggaaattgtt cggccagttg
    13141 tactaaattt ctttcaatcg acgcatctcc ggcacctaac acagcaagtt gcgcgggcaa
    13201 ttcaaggagt ttaggtgcaa cctcaaatag caagtcatat cctttttgat aactaaagcg
    13261 actgatcgca gcgaacagta actgatcaga tcggggctcc agtcctagct tttgctgaat
    13321 cgccagttta ttggcttgct tattggataa acgacgaatg gtatactttt tagcgatatg
    13381 aatatcagcg gatggatccc actcgatggt gtcaatccca ttcacaatcc cactgatc
//
LOCUS       DAOQKQ010000063        13275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_2192_length_13275_cov_5.987519, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000063 DAOQKQ010000000
VERSION     DAOQKQ010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 13275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13275
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2192_length_13275_cov_5.987519"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1246)
                     /locus_tag="PKZ49_05675"
     CDS             complement(<1..1246)
                     /locus_tag="PKZ49_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HND36085.1"
                     /translation="MHKLVNKVSLVVLGIIAGVMISLNFSAVADKTLKDELPALPIEE
                     LRAFTQVFGRIKSDYVEPVDDKKLITEAINGMLVGLDPHSAYLDSEEYKELQIGTQGE
                     FGGLGIQVTMEDGLVKVISPIEDTPAFRAGIKTGDLIIKLDDTAVKGMTLNEAIKRMR
                     GKPDTSITITIAREGEAEPLVFTLVRAIIKIESVKSKLIEPGYGYVRITQFQEQTGEN
                     LAKAIDKLFKESKIPMKGLVLDLRNDPGGLLNSAVGVSAAFLPANALVVYTEGRTTDA
                     KMRLLANPEYYLRGPGKDYLTGLPDAVKTVPMVTLVNGGSASASEIVAGALQDHKRSI
                     VMGTQTFGKGSVQTILPLGNNTAIKLTTARYYTPNGQSIQAKGITPDIVDESEESSRQ
                     LRESDLRRHLRSNKSESGDDNTN"
     gene            complement(1286..2545)
                     /locus_tag="PKZ49_05680"
     CDS             complement(1286..2545)
                     /locus_tag="PKZ49_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HND36086.1"
                     /translation="MEQTHRVGKALRCWFFKAIPLMIVLAIAYQPFFLFTHVDAAPQP
                     SKEDLLALQNRIKALQDELAAKEVHKVEATDALQESERAISEINSVLVKLAKQQTDAN
                     NRLNELMHQTRQLEEELNQQTDQLGSLLYHQYINNRSSNFHLLLNLQDPNHIARNLYY
                     YRYLKQARVESIVHLQQYLHDIEMLKSESRVKQDEIAKVQAEQTQQKKILDQEKAKRA
                     TVLANLSEKISLQQHELSKLKADEQRLAKLVEQINQFLAKSKRDSDTASTSANAPLLN
                     NKTPDSTVSGYSFTSLRGKLRLPVRGELVNRFDSPRQEGGIKWRGLFIRAKNGNEVKA
                     IASGQVVFADWLRGFGNLMIVDHGSSYMSLYGYNEAIYRRVGDKVQGGDTIAIVGNSG
                     GSSESGLYFELRHQGKPFDPLTWVKIE"
     gene            complement(2565..3326)
                     /gene="gpmA"
                     /locus_tag="PKZ49_05685"
     CDS             complement(2565..3326)
                     /gene="gpmA"
                     /locus_tag="PKZ49_05685"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HND36087.1"
                     /translation="MDMIKLVLLRHGESTWNNENRFTGWTDVDLTARGQEEAKAAGKI
                     LRQNGFAFDVAYTSVLKRAIRTLWIVLDEMDQMWIPITHSWRLNERHYGALQGLNKAE
                     TAARYGDEQVLIWRRGYDVLPPVLTIDDERYPGFDPRYSKLTKEEIPLAESLKSTVTR
                     FLPFWNQTIIPDLQAGRSVIIAAHGNSLRALIKYLDNVSDEAIIDYNIPTGIPLVYEL
                     DKQLKPINNYYLGNQAEIEEAMLAVANQGKVHKNS"
     gene            complement(3436..6492)
                     /locus_tag="PKZ49_05690"
     CDS             complement(3436..6492)
                     /locus_tag="PKZ49_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HND36088.1"
                     /translation="MNLAALCIQRPIMTILLSLTAVILGLTAYDGIPIAALPNYDTPT
                     ISVSAVLPGASPETMAAAVATPLERQFASIADLSTISSTNTQGNTQITLEFNQGRDMD
                     SAAVDVQAALLRAQRALPIEMTTMPSYRKINPADTTIAILSLTSETLLFSELNQFADK
                     LIIPALSTLPGIGEVRISGQKKFAVRIRVNPLALAARNLTIDDIAKVIADANPNSPLG
                     TLDGSRQTLTIQANRQLEHATDFANLIVSADENGRLIRLSELAIVENSFESVKTASWT
                     NGQPSITLLIYAQPGANVVAAVDAMKRGLPGINAQLPGSAQLSFYLDRSQSIREAIFD
                     VQFTFGLTVGLVILVMFLFLRKPNVTLIPALSLPISILCTIALMRVLGYTLDNISLLA
                     ITLAVGLVVDDAIVVLENIVRYIEKGLSPIRAALLGSKEITFTILSISVSLVAVFIPI
                     FFMPGVIGMLFHEFAAVVSIAVLVSAIVSLTLVPMLASRYLIQHESSTKVLVLTQWFE
                     SGFERLLTAYEYLLDWALTHRRIVLLIAISTLIVTVVLFSVLPKGFFPEEDTGRIMIV
                     IEAAEDISFPAMVELVRQTMEKIRSNPLIDNSVAFTEGAANNARMFVGLIPRSERPSM
                     TEVVQILRAETQSIPGVNVFMTPTQHLRLGSMPSKSRYQYVMRSIHSEELQTAATLMT
                     DQMRKHPVFRDVTNDLKLRGLQARLEIDRDKANQLGIDMASIRTALYSAFGERQVSTI
                     YTSSDSYRVILEVADAFRDNEKALNNLHLRSQTGGLVPLSSIAKVNREIGPLSVNHVN
                     QLESITLSFNLSAGSALGDATQLIKQFEREIQLSSSVLTNFAGDAAAFQSTQVSQLVL
                     ILAALLVIYVLLGVLYESFIHPLTILSGLPSAAVGALGILWLFDMELSVIAMIGILLL
                     IGIVKKNAIMMIDFALDAQRRLNMVPHQAIRTACLLRFRPIMMTTLAALMGALPIAIG
                     FGAGAELRQPLGLTVVGGLLFSQLITLIITPVIYLYLDPESKKTRG"
     gene            complement(6506..7744)
                     /locus_tag="PKZ49_05695"
     CDS             complement(6506..7744)
                     /locus_tag="PKZ49_05695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HND36089.1"
                     /translation="MTEITDNKKSVGGFRRLLSKWIVVVVAIAIVLLSQQYFDHSSKS
                     NEAKPKNKVAVVLTRVEQQPVAIQIKMKATVHPKQTIVVRPQLSAVIKSVHIKEGQLV
                     KQGDKLFSFDTRDREANLAKSKAQLSKTKAELAIAQRNYQRQQALFRKNFISQAMLDV
                     ARNQVELLQNQLKFDQAAIQADQVSLSYGEIYAPISGRVGSIPFFAGSLVQPESSQLV
                     SIVQLDPIQVSFALPESELSLLQRSSTLGQIKAIVQFGSSGEETREGKVVFVDNAIDT
                     ASGTIQIKAELTNADGKLWPGMYVEVVLVKQWIPDAIVVPVQAVQSGPMGHYVFMIDT
                     TDKAIMQPISVRFIQEGIAIVDGLDFGYCIVVEGAHALLSGSTIGENSLTQPSDKMCK
                     QVGFTESSRSTSTVEMQQSK"
     gene            complement(7785..8498)
                     /locus_tag="PKZ49_05700"
     CDS             complement(7785..8498)
                     /locus_tag="PKZ49_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008059060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HND36090.1"
                     /translation="MLRNIFIKNRHTQRDKQVIVQATALNKTVALGDKPLIILQDINL
                     QINAGESVAIVGASGSGKSTLLGLLAGLDVPTSGQVFLDTEAIFLLDEDQRAALRSRL
                     LGFMFQSFQLLPGLTAMENVMLPIELSGLKSAKQLAQALLERVGLGARLDHYPRQLSG
                     GEQQRVAIARAFVTQPKLLFADEPTGNLDSETGKQIIELMFELNQEKGTTLILVTHDE
                     DLSNRCDRKIKMVAGRIID"
     gene            8479..9129
                     /locus_tag="PKZ49_05705"
     CDS             8479..9129
                     /locus_tag="PKZ49_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HND36091.1"
                     /translation="MKIFLSIIFLFINLIILTPTGAESHQNRITIMILGDSLSAGYGL
                     PTESSWVTLLKNRLQNESPNYHVINISISGETTLGGRNRIEQALKLHEPNIVIIGLGA
                     NDGLRGTSIQTINDNLEMIIETCQRNHAAPILVGMQLPPNYGSTYTQKFRDLYQHIAT
                     RYQLPLIPFLLEGFADKRDHFLADGIHPNTAAQALILENVWKVLNPLIKTQMQKEG"
     gene            complement(9167..10132)
                     /locus_tag="PKZ49_05710"
     CDS             complement(9167..10132)
                     /locus_tag="PKZ49_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HND36092.1"
                     /translation="MPNAVYVENIDERSEGQRIDNFLFKHLKGVPKSHIYQILRSGQV
                     RVNSKRVTADYRLQLHDAVRIPPLKLTLKSEPTRILPRANSTRFEKLYEDEWLIAINK
                     PAGMAVHGGSGVSYGIIEQLRQDYPEWKFLELVHRLDRDTSGVLMLAKKRSALVGLHQ
                     QIREGSVQKFYWVIVKGKWTNAKQSVKLLLNKFVTAKGERRVTVTDGRNQDTKDLFSH
                     TIFTLEQSWPDYSLLKADLKTGRTHQIRVHLAHLGFPIVGDEKYGDFDFNRQLMKKHQ
                     KIKRMFLHAGTIAFIHPITNVALKVTAPLSKEWDQFIDWFKREGG"
     gene            10425..10559
                     /locus_tag="PKZ49_05715"
     CDS             10425..10559
                     /locus_tag="PKZ49_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36093.1"
                     /translation="MNKSLPDAAASRKFDFHRNNTLDLGKQKNGFMTYSADDLTITGN
                     "
     gene            10754..>13275
                     /locus_tag="PKZ49_05720"
     CDS             10754..>13275
                     /locus_tag="PKZ49_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HND36094.1"
                     /translation="MKRMLFNATQPEELRVAIVDGQKLIDLDIESVGKEQRKSNIYKA
                     VITRLEPGLEAAFVDYGGDRHGFLPYKEIDLSVIGNQSETPSHRIHEHLREGQELIVQ
                     IDKDERGNKGAALTTYISLAGRYLVLMPNNPRGGGISRRVNADERAELRDIIGRLQIP
                     DGMSIIARTAGIGRNEEELQWDLNYLTQLWHAIEEAANNQGGVFLIYQESSLVIRAIR
                     DYFYHEIGEILIDNPEIYDQARQFMTHVMPNNVDRLKLYQDDVPLFSRFQIEHQIETA
                     YSRQVSLPSGGSIVIDHTEALVSIDVNSAKAIRGTDIENTALNTNLEAADEIARQLRL
                     RDLGGLIVIDFIDMEVARNQREVEIRLQEALHVDRARIQVGKISRFGLLELSRQRLRP
                     SLGESNYITCTRCHGTGHIRGTDSSALHVLRIVQEEAMKENTSALHVQLPVDVATYLL
                     NEKRAEIHNIEARLKVNIILIPNIYLETPNYKINRLRHEDIKSVEVQASYEMVEKVAE
                     EITLPSISPENKVTRPQAAVKGITLAQSAPIREEKPISFLDKFFSWFRPTTEEQNKSE
                     TASIHAREKSSQRERGRGRRDRGRGGRNKETPANDQRKRNNLNEVHKEAHQDTESILL
                     QESRSDLTAYHDETLVSQPKDNQSSQLVHSSVELASNQPEIHELGSTEGNIKQSEQTE
                     EKSRPRRRRGGRQRNRSDRPRSTATSETLETTSHAVDAIPSIAIASTEPFEIVARSDF
                     EAVNSTDHQSQPMNSDAWAEHRLQEVPSEKIERTEDQASSIKAANSVTQDELSVDSAS
                     SFKPVTVTAPVVIDLPDLSKSGLVMIETTQENVSEILDDEML"
BASE COUNT         3973 a         2983 c         2519 g         3800 t
ORIGIN      
        1 cattcgtatt atcatcacca ctttcactct tgttactgcg taaatggcgt cttagatctg
       61 attcacgaag ttggcgtgag ctttcttctg attcatcgac aatatccggt gtaataccct
      121 tagcctgaat cgattgccca ttcggcgtat agtatcgtgc tgtcgttaat ttgattgcgg
      181 tattattccc cagcggtaga atcgtttgca cagaaccttt accaaaagtt tgtgttccca
      241 taacaatgga acgtttgtga tcttgcaatg cccctgcaac aatttcagaa gcagaagcag
      301 atcctccatt cactaaagtt accattggca cagttttaac cgcatccggt aaccctgtta
      361 gataatcttt gcccggtcct cttaaataat actcaggatt agctaatagc cgcatttttg
      421 catccgtagt acgcccttcg gtatagacta ccaaggcatt agcaggtaaa aaagcagctg
      481 acaccccaac agcactattt aataacccac cgggatcatt gcgtaaatca agaacaaggc
      541 ctttcatcgg aattttgctt tctttaaata atttatcgat tgccttagcc aaattttccc
      601 cggtttgctc ttgaaactgg gtaattctta catacccata accgggttcg attaattttg
      661 atttaacgct ttcgattttg atgatagctc gaactaatgt gaaaaccaaa ggctcagctt
      721 caccttctcg agcaatcgtg atagtaatcg aagtatctgg cttaccgcgc atccgcttaa
      781 tcgcctcatt caatgtcatt ccttttacgg cagtatcatc cagtttaatg attaaatcac
      841 ctgttttaat ccctgcgcga aaagctggtg tatcttcaat cggtgagatt actttaacca
      901 gaccgtcttc cattgtcacc tgaataccga gtcccccaaa ttcaccctgt gtaccaattt
      961 gtaattcttt atattcttca ctatccagat aggcagagtg gggatcaagc ccaacaagca
     1021 tcccattaat cgcttcagtg atcagtttct tatcatcaac tggctcaaca taatcgctct
     1081 tgatgcgacc aaaaacttga gtaaacgctc gtaattcctc gattggaaga gctggaagct
     1141 cgtcttttag ggttttatca gcgactgcag aaaaattaag gctgatcatt acccccgcga
     1201 taatacctaa tacaaccaag ctaactttat taacaagctt atgcattact tcctcaccta
     1261 tcgactataa tcattaatct caaattcatt ctattttgac ccaggtcagg ggatcaaatg
     1321 gtttaccctg atgtcgtaat tcaaagtata accctgattc acttgatccg ccgctattcc
     1381 ctacaattgc aatggtatcc ccaccttgta ctttatcacc aacacgtcta taaattgctt
     1441 cattataacc atataaactc atgtagctac taccatgatc aacaatcatt aaattaccaa
     1501 atcctcttaa ccaatcagca aaaaccactt gaccgctcgc aatcgccttt acttcgttgc
     1561 catttttagc gcgaataaat aagcctcgcc atttaattcc tccttcttga cgtggactat
     1621 cgaatcgatt gacaagttcc cctcgaactg gtaaacgcaa cttacctctt aaggaggtaa
     1681 aactatagcc actcacagta gaatctggag ttttattatt caataacgga gcattcgctg
     1741 atgtactagc tgtatcgcta tcacgcttgg atttagcaag gaattgatta atttgttcga
     1801 caagtttagc aaggcgttgc tcatcagctt tcagcttact caattcatgc tgctgcaagg
     1861 aaattttttc tgaaagattt gctaaaactg ttgcacgttt ggctttctct tgatctaaaa
     1921 ttttcttttg ctgagtttgc tcagcctgta ctttagcaat ttcatcttgt tttactcgac
     1981 tttcgctttt taacatctcg atatcatgca aatattgttg taaatgcacg atactttcaa
     2041 cacgcgcttg ctttaaatat cgatagtaat aaagattccg cgcaatatga ttcggatctt
     2101 gtagatttaa cagtaaatgg aaattgctgg atcgattatt aatatattga tgatacagta
     2161 aagaaccgag ttgatcggtt tgttgattaa gttcttcttc aagttgacgg gtttgatgca
     2221 tgagctcatt taagcgatta tttgcatcag tttgttgctt tgcaagcttc acgagtacgc
     2281 tattaatttc actaattgca cgttctgatt cttgtagcgc atcggtagcc tcaactttat
     2341 gcacttcttt cgctgccagt tcatcttgaa gtgcttttat tcgattttgt aacgctaata
     2401 aatcctcttt actcggttgc ggtgctgcat caacatgcgt aaaaagaaaa aagggttggt
     2461 aggcaatcgc caatactatc attagcggaa ttgccttgaa gaaccagcat ctcaaggcct
     2521 ttccaacacg atgagtttgt tccatttcag ggattggaac ttaatcaact attcttgtga
     2581 actttacctt gattcgcgac agcaagcatt gcttcttcaa tctcagcctg attacccaaa
     2641 tagtaattat tgattggctt taattgttta tctagttcgt aaactaatgg aatgcctgtc
     2701 ggaatgttat agtcgataat cgcttcatca gaaacattat ccaaatattt tatgagggct
     2761 ctgagtgagt tgccatgcgc tgcgataatc acactccgtc ccgcttgcaa atcaggaatg
     2821 atggtttgat tccagaatgg taagaatcga gtaaccgtac tctttaaact ctctgctaac
     2881 gggatttctt ctttggtgag cttactgtaa cgaggatcaa aaccagggta tcgctcatca
     2941 tcgatcgtta aaacaggggg caatacatca tagcctcgcc gccaaataag cacttgttca
     3001 tcaccataac gtgcagccgt ttcagcttta tttaaacctt gaagtgcacc atagtgccgt
     3061 tcattcaatc tccaagaatg cgtaatggga atccacattt ggtccatctc atccaacaca
     3121 atccataatg ttcggattgc acgtttaagc acagaagtat aggcaacatc aaaagcaaat
     3181 ccattttgac gcaatatctt tcctgccgct ttcgcttctt cttgacctct tgccgttaaa
     3241 tcgacatctg tccaacctgt aaaacgattt tcattattcc aagtactttc tccatgacgc
     3301 aggagaacaa gtttaatcat gtcaatatct taagagtggg taatttctaa ggagcaattc
     3361 aataatcggt aattctatta catttgacct aaaaacgcag caaagatagg catagaattc
     3421 atcgcttttc tccgattatc ctcgagtttt tttggattca ggatcgagat ataagtagat
     3481 gacgggggta ataatcaaag taatcaattg agaaaaaagt aatccgccaa caaccgttag
     3541 acctagcggt tgacgtaatt cagcacctgc accaaaaccg atcgcaatcg gtaacgcacc
     3601 cattaatgct gctagtgtcg tcatcataat cggacgaaag cgaagcagac aagctgttcg
     3661 aatcgcttgg tgaggaacca tgttcaatct gcgctgcgca tctaaagcaa aatcgatcat
     3721 cataattgcg ttctttttca caatcccaat caacaaaaga attccgatca tagcaatcac
     3781 cgataactcc atatcgaata gccacaaaat acctaacgcc ccaactgcag cagaaggtaa
     3841 tcccgaaagg atagtgagcg gatggataaa actttcatac agcacaccca ataaaacata
     3901 aatcaccaat aaagcagcca agataagtac caattggctc acttgggtcg attggaaagc
     3961 cgctgcatca cccgcaaaat tcgttaatac cgatgaagat aattgaatct cacgctcaaa
     4021 ttgcttaatt aattgcgttg catccccaag tgccgatccc gctgaaagat taaacgatag
     4081 ggtaattgat tcgagctgat ttacatgatt aacgctcaaa ggacctattt ctcgatttac
     4141 tttagcaata ctcgataaag gaaccaagcc cccagtctgt gagcgtaagt gtaaattatt
     4201 taatgctttc tcattgtccc ggaaagcatc agccacttcc aaaataaccc gatagctatc
     4261 actggatgta taaatggttg aaacttgcct ttccccaaaa gcgctgtata aagcagtacg
     4321 aattgaagcc atatcaattc ccaattgatt cgctttatct cgatcaatct ccagtcgtgc
     4381 ctgtaatcct ctgagtttaa gatcattcgt tacatcgcgg aataccggat gtttacgcat
     4441 ttgatccgtc atcagggttg ctgcggtctg caattcttca ctatgaatgc tgcgcatcac
     4501 atattggtat cgacttttac tcggcatact ccctaacctt aaatgctgtg tcggggtcat
     4561 aaaaacattt acgcctggaa tcgattgagt ttcagcacgc agaatctgaa caacctctgt
     4621 catcgaagga cgttcactac gagggattaa tcctacaaac atacgcgcat tgttcgcagc
     4681 accttccgta aatgcaacac tgttatcgat caacggattc gatcgaatct tttccatggt
     4741 ttgtcgaacc aattccacca tcgccggaaa agaaatatcc tcagcagcct cgattacgat
     4801 catgattcga ccggtgtctt cttcaggaaa gaacccttta ggtagcacac taaaaagaac
     4861 gacagtaact attaaggtac tgatagcaat taatagtaca atccgacgat gggttaaggc
     4921 ccagtctaat agatattcat acgctgtcag aagtcgttca aaccctgatt caaaccattg
     4981 agtcaatacc aataccttcg ttgaagattc gtgttgtatc aagtaccgac ttgctaacat
     5041 tggcaccaat gtcaatgaaa caattgctga caccaatacg gctatactta ctactgcagc
     5101 aaactcatgg aataacatgc caattacgcc cggcataaaa aatatgggaa taaaaacggc
     5161 aaccagcgaa accgaaatcg acaaaatggt aaatgtgatt tcttttgagc ccagtaatgc
     5221 agcccttatt ggggacaagc ctttttcgat ataccgaaca atattttcca gaactacgat
     5281 tgcatcatca acgactaaac ccactgcgag tgtaatcgcg agtaatgaaa tattatcgag
     5341 cgtataaccc agaacacgca tcaaggcaat cgtgcataat attgaaatgg gtagtgataa
     5401 agcgggaatt aaagtgacat taggtttacg taaaaacaga aacatcacta gaataactaa
     5461 acccacggta agcccgaagg taaattgcac atcgaaaatc gcttcacgaa tcgactgtga
     5521 gcgatccaaa taaaatgata actgtgccga cccaggtagt tgagcgttaa ttcctggcaa
     5581 ccctcttttc atggcatcga ctgcagcaac cacattggca ccaggctgtg cataaattaa
     5641 caacgtaatg gagggctgcc cattggtcca gcttgcggtt tttacagact caaaactatt
     5701 ctcaactatt gcaagctcag acaatcgaat taatcgtcca ttttcatccg ccgacacaat
     5761 aagattagcg aaatcagtcg catgctctaa ttgacgatta gcttgtatgg taagcgtctg
     5821 acggctaccg tcgagggtac ccaatggaga attgggattt gcatctgcga taactttggc
     5881 aatatcatca atcgttaagt tacgcgcagc aagtgcgaga ggattgacac ggatacgaac
     5941 tgcaaacttt ttctggccac taatccgaac ttcaccaatc cctggtaatg ttgataaagc
     6001 aggaataatt aacttatcgg cgaactgatt taattcagaa aagagtaaag tttctgatgt
     6061 aagcgaaaga atggcaatcg tagtatctgc aggatttatc tttcgatagg atggcattgt
     6121 tgtcatttca atcggcaatg cccgttgcgc tctcagcaat gctgcttgaa catcgacagc
     6181 agcactatcc atatcacgac cttgattaaa ctccagagta atttgcgtat taccttgggt
     6241 attcgttgaa ctgattgtag ataaatccgc tattgaagca aactgacgct ccagtggtgt
     6301 tgccactgct gcagccatgg tttcaggact tgcaccgggc agtacagcac ttaccgatat
     6361 ggtgggtgta tcataattag gtaaagctgc aatcggaatt ccatcgtacg cagtcaaccc
     6421 aagaatgacc gcagttaatg acaaaagtat cgtcatgatg ggacgttgaa tacacaatgc
     6481 cgcgagattc atcgattacc taaggttatt tagattgctg catttccact gtactcgtgg
     6541 aacgcgaaga ctcagtaaat cctacttgct tacacatttt atcgctcggc tgtgttaatg
     6601 aattttcacc aatcgtactg cctgaaagaa gtgcatgagc accttccaca acaatacaat
     6661 aaccaaaatc gagaccatcc actatcgcga tcccttcctg aataaatctt acggatatcg
     6721 gttgcataat cgctttatca gttgtgtcga tcataaacac ataatgaccc attggacctg
     6781 attgcactgc ctgtactggg acaacaatag catcaggaat ccactgcttg acaagaacta
     6841 cttcaacata catgccaggc caaagttttc catctgcgtt tgtaagctcg gctttaattt
     6901 gaatcgtgcc actcgccgta tcgatcgcgt tgtcaacaaa gacaactttt ccctcccggg
     6961 tttcctcgcc ggaagatcca aattgtacga tggctttaat ttgacccaat gtcgatgatc
     7021 gttgcaataa acttaattcg ctttctggaa gcgcaaaact cacctggatc ggatcaagtt
     7081 gcacaatact cactagctga gatgactcag gctgaactaa gctgccagcg aaaaatggta
     7141 tgctcccaac gcgcccacta atcggcgcat aaatttcccc ataactcaaa ctaacctgat
     7201 ctgcctgaat agcggcttga tcaaatttaa gctgattctg gagtaactcg acttgatttc
     7261 ttgccacatc caacatcgcc tgtgaaataa aattcttcct aaataatgct tgttgacgct
     7321 gatagttacg ctgcgctatc gctaattcag ccttggtttt tgatagctga gcctttgact
     7381 tcgctagatt ggcttcacgg tctcgcgtat cgaaagaaaa caacttatcg ccctgcttca
     7441 cgagctgtcc ttctttgata tgaacagatt taatgacagc actcaattga ggacgcacca
     7501 caatggtttg cttaggatgc accgttgctt tcatcttaat ctggattgct acgggctgtt
     7561 gctcaacgcg cgttaatacc actgccactt tatttttcgg tttagcttca tttgatttag
     7621 aactgtggtc gaaatattgc tgactcaata aaacgatcgc aatggccaca accacaacaa
     7681 tccatttgga aagaagacgc ctaaaacccc ctacactttt tttattgtcg gtaatctcag
     7741 tcacgtcaaa tacctatttc gccctaaacg atcgaatcag tcacttaatc aatgattcga
     7801 ccagcaacca ttttgatttt tcggtcacat cgattcgata agtcctcatc atgggtaacc
     7861 agtattaatg ttgtcccttt ttcttgattc aattcgaaca tcaactcaat aatctgttta
     7921 ccggtttctg aatctagatt accggttggt tcatcagcaa ataacaattt gggctgcgtc
     7981 acgaaagctc gagcaatcgc aacgcgttgt tgttcgccac ccgaaagctg cctaggataa
     8041 tgatcaagac gcgcgcctaa acctaccctt tcaagtagtg cttgagccaa ttgttttgca
     8101 gattttagcc ccgaaagttc tatcggtagc attacatttt ccattgctgt taaccctggc
     8161 aacaattgaa aagattgaaa cataaagcca agtagtcgcg aacgaagtgc tgcacgctga
     8221 tcttcatcaa gtagaaaaat agcttcagta tctagaaaaa cttgaccact ggttggcacg
     8281 tcgagacctg ctaataaccc tagcagggtt gatttaccag aacctgatgc acccacaata
     8341 gcaaccgatt cccccgcatt gatttgaaga ttgatatcct gcaggatgat caatggctta
     8401 tcacccaggg caaccgtttt atttagcgca gttgcttgta caataacttg tttatctctt
     8461 tgggtatggc ggttttttat gaaaatattt cttagcatta tttttctatt tattaatctt
     8521 attattctga ctcctaccgg agctgaatcc catcaaaatc gaataaccat catgattctg
     8581 ggtgatagct tgtcagcagg ttacggacta ccaaccgaat ccagttgggt cacgttactt
     8641 aaaaatagac tacaaaatga atcacccaac tatcatgtta ttaatattag tatcagcggg
     8701 gaaacaacac ttggaggtcg caaccgaatt gaacaagcgc tcaaattaca tgaacccaat
     8761 atcgttatca ttggtttagg cgctaacgat gggttacgag gcacttccat ccaaacgatt
     8821 aatgataact tagaaatgat catcgaaacg tgccaacgca atcatgctgc accaatattg
     8881 gttggaatgc agcttccgcc gaattatggc agcacttata cacaaaagtt ccgtgatctc
     8941 taccaacaca ttgctacacg ctatcaacta ccgcttatcc catttttact cgaaggattt
     9001 gcagataaac gtgaccactt tcttgcggat ggaattcatc ccaatacagc agctcaagcg
     9061 ctcattcttg aaaatgtttg gaaagtgctc aaccctttaa tcaaaactca aatgcaaaaa
     9121 gaaggctaaa cccttaatct attctagaga aaatatttaa attcctttaa ccgccctctc
     9181 gtttaaacca atcaataaat tgatcccatt ctttagacaa aggagccgtc acttttaaag
     9241 caacgtttgt tataggatgt ataaacgcta tcgtccccgc atgtaaaaac atccttttaa
     9301 ttttttgatg cttcttcatc aactgtcgat tgaagtcaaa atcaccgtat ttctcatcac
     9361 cgacaattgg aaagcctaag tgtgccaaat gaacacgaat ttggtgtgtt cgtcctgttt
     9421 ttaaatccgc ttttagcaaa ctataatcag gccagctttg ttcaagtgta aatatcgtgt
     9481 gtgaaaatag atctttcgta tcttgatttc ttccatctgt aacagtgaca cgtcgttcac
     9541 ccttcgctgt cacaaatttg tttaataaca atttcacgct ctgtttggca ttagtccact
     9601 ttcctttgac tatcacccaa taaaacttct gaacggaacc ctcacgaatc tgctggtgta
     9661 ggcccactaa agcgctacgc ttcttagcca acattaaaac acctgaggta tcccgatcaa
     9721 ggcggtgtac taattcaaga aacttccact cggggtaatc ctgccgcagt tgctcgatta
     9781 ttccataact gacaccacta ccaccgtgaa ccgccattcc tgccggttta ttgatagcaa
     9841 tcagccattc atcttcatat aatttctcga aacgggtaga attagcgcgt ggtagaattc
     9901 ttgttggttc tgattttaag gttaacttaa gcggtggaat acgaacggcg tcatgcaatt
     9961 gtaaccggta atctgccgta actcgtttgc tattgacgcg aacttggcca ctgcgcaaga
    10021 tttgataaat gtggctttta ggcacacctt tcaagtgctt aaaaagaaaa ttatcgatac
    10081 gttgaccttc cgatctttca tcaatatttt ccacgtaaac tgcatttggc atattttctt
    10141 tgcctaacac tttaatttta cctatacttt taggttgagt aattaattga attgatatac
    10201 ctatatcatt taatgtgtca aatcgttgac atcatggctc atgctaaaca agatttagcc
    10261 ataggtagat ttggcaatgc tgaaaagtcg gcaccaaatc tggttactgt cgctcacctg
    10321 atcaagtagc gataataaat gaattacgcg ctcaaagcac ttcaaggatt tatttacaaa
    10381 ttttgtagta ccttacagta ggaataactt tgccgaaatt tgccatgaat aaatccttac
    10441 ctgatgctgc agcaagtaga aaatttgact ttcaccgcaa taacacacta gacctgggta
    10501 aacaaaagaa cgggttcatg acgtatagtg cggatgattt aacgattaca ggaaactaag
    10561 ttagtgttaa cggcatttat tgaaatgttg atttaacttg gtaaattact tactcgaatg
    10621 atagggaaac gacaaaccca attattgtat ttatgattta tgtttactcg atcacctgaa
    10681 aatatgtcat tccctttgga actacttagc tgatattagc aacctgcccg tgaattgagc
    10741 gtgggaggaa ataatgaaac gaatgttatt taatgcaaca caacctgaag agttgcgtgt
    10801 tgcaattgta gatggacaaa aactaattga tctcgatatt gaatcagtcg gaaaggagca
    10861 acgaaaaagc aatatttaca aagcggtaat cactcgacta gaacccggtt tagaagcagc
    10921 ttttgtcgat tatggtggcg atcgccatgg atttcttccc tataaggaaa ttgatttatc
    10981 ggtcatcggt aatcaatctg aaaccccaag tcatcgcatt catgaacacc ttcgtgaagg
    11041 acaggaatta atcgtccaga tcgataaaga cgaacggggt aataaaggag cagcgctcac
    11101 tacctacatc tcattagcag gccgctatct tgtgctcatg cccaataatc cacggggtgg
    11161 tggcatttca cggcgagtta atgccgacga gcgtgctgaa ctgagagaca ttattggtcg
    11221 gctccaaata ccagacggca tgagtatcat tgctcggaca gccggaattg gacgtaatga
    11281 agaagagctg caatgggact taaattattt gacacagctc tggcatgcga tcgaagaagc
    11341 ggcgaataat cagggtggtg tgttcttaat ttaccaagaa agcagcttag tcatccgcgc
    11401 gattcgtgac tatttttatc atgaaatcgg tgaaatattg atcgataacc ctgaaatcta
    11461 tgatcaggcg cgtcaattta tgactcatgt catgcctaat aatgtagatc gactcaagct
    11521 ctaccaggat gatgttccct tattctctcg tttccaaatt gaacaccaaa tcgaaaccgc
    11581 ctattcaagg caagtttcgc taccgtctgg tggatcgatc gttatcgatc atactgaagc
    11641 attggtgtca attgatgtta attctgcaaa agccattcga ggcacggata tcgaaaatac
    11701 cgccttgaat actaacttag aagctgcaga tgagattgct cggcaattaa gactccgcga
    11761 tttaggcggg ctcatcgtaa tcgattttat cgatatggag gttgcacgaa atcaacgaga
    11821 ggtggaaata cgcctgcaag aagctttgca tgttgatcgt gcacgaattc aagtcggtaa
    11881 aatttcacgc tttggtctat tagaattatc tcgtcaacga ctacgccctt cattaggcga
    11941 aagtaattat atcacttgca cgcgttgcca tggcacagga catatacgtg gaacagattc
    12001 atctgcttta catgttcttc ggattgttca agaagaagcc atgaaagaaa acacgagtgc
    12061 tttgcatgtt caattacccg ttgatgtcgc aacctatctg ctaaacgaga aacgtgcaga
    12121 aattcataac attgaagcac gcctcaaagt aaatatcatc ctcattccca atatttattt
    12181 agagacaccg aattacaaaa ttaaccgact ccgtcatgag gacatcaagt cagttgaagt
    12241 tcaagctagt tatgaaatgg ttgaaaaagt agcagaggaa ataacgctcc cttcgatttc
    12301 accagaaaat aaagttacac gtcctcaagc agctgttaaa ggcatcactt tggctcaatc
    12361 tgcaccaatt cgtgaagaaa aaccaatttc ttttttagat aaattcttca gttggttcag
    12421 accgacaacc gaggagcaaa ataaatcaga aactgcatct attcacgctc gggaaaaatc
    12481 ttctcagcgt gagcgaggaa gaggacgtag agatcgtggg cgtggtggtc gaaacaaaga
    12541 gacacctgcc aatgatcaac gcaaacgtaa taatctaaat gaggtccata aagaagcaca
    12601 tcaagataca gagagtattc tgttacaaga atcgcgatcc gatcttactg cctatcatga
    12661 tgagacgtta gtgagccaac ccaaagataa tcagagttct caactcgtac acagctcagt
    12721 cgaactagca tcgaatcaac ctgaaataca tgaattgggg tcaaccgaag gcaacattaa
    12781 acaaagtgag caaactgaag aaaaaagccg tccacgccga cgtcggggag gcaggcagcg
    12841 taatcggtct gatcggccta gatcgaccgc taccagtgaa acgctagaaa cgacttctca
    12901 tgcagtagat gcgatccctt caatcgctat cgcatcgact gaaccctttg aaatagtagc
    12961 acggtcggat ttcgaagcag tcaattcaac tgatcatcag tctcaaccga tgaactctga
    13021 tgcttgggct gagcatcgat tacaagaagt cccgtctgaa aaaattgaac gcactgaaga
    13081 tcaagcttct tctattaagg cagctaactc agttacgcaa gatgaactct cagttgattc
    13141 agcatcttca tttaaaccgg taacggttac tgcaccagtg gtgatcgatt tacccgattt
    13201 atccaaaagt ggactcgtta tgattgaaac tactcaggaa aatgtatcag aaattttaga
    13261 tgatgaaatg ctatt
//
LOCUS       DAOQKQ010000064        13147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_2218_length_13147_cov_6.043614, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000064 DAOQKQ010000000
VERSION     DAOQKQ010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 13147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13147
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2218_length_13147_cov_6.043614"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PKZ49_05725"
     CDS             complement(<1..388)
                     /locus_tag="PKZ49_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36095.1"
                     /translation="MTLMTLSNNDYINSLVQRWGSYSNSTNVAWLNQLRSEALHCVNK
                     LRMPTTHMEAWRFTDLSALAKLSFPEALLFPRDQLGELARFFLSESESEYRLVFIDGH
                     YVAELSQIDSSEKIIQGHLSSLILSHN"
     gene            complement(385..1182)
                     /gene="sufC"
                     /locus_tag="PKZ49_05730"
     CDS             complement(385..1182)
                     /gene="sufC"
                     /locus_tag="PKZ49_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HND36096.1"
                     /translation="MIDQNSKTLLEVRDLHAKVSDVEILKGVNLTVKCGEIHAIMGLN
                     GSGKSTFAKVLAGHPGYQVTAGSVSFDNQDLLSLEPEVRSRNGIFLAFQYPIEIPGVA
                     NSQFLRLAYNTNQLSRGKEELDPLEFDDFVREKMQLLEMNVDFLDRSVNEGFSGGEKK
                     RNEILQMALLEPKLAILDETDSGLDIDALRIVASGVNKLTNKDNAIILVTHYQRLLDY
                     IVPDYVHIMESGRIIKTSGKDLAKELEVHGYDWIKADQLAKSAESSI"
     gene            complement(1179..2615)
                     /gene="sufB"
                     /locus_tag="PKZ49_05735"
     CDS             complement(1179..2615)
                     /gene="sufB"
                     /locus_tag="PKZ49_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HND36097.1"
                     /translation="MSALLQNLVNQPYKHGFVTEIESDIAPKGLNEEIIRMISAKKNE
                     PDWLLSFRLRAYEKWLSMAEPQWQNVKYPKIDFQDISYYSAPKPKKKLASMDEVDPEL
                     LRTFEKLGVPLHERAALAGVAVDVIFDSVSVATTFKEKLAEVGIIFCSISEAVQNYPE
                     LVQKYLGSVVPVGDNFYAALNSAVFTDGSFCYIPEGVKCPMDLSTYFRINTEGSGQFE
                     RTLIIAENGASVSYLEGCTAPRFDTNQLHAAVVELVALDNAEIKYSTVQNWYAGDEKG
                     IGGIYNFVTKRGLAKGINSRISWTQVETGSAITWKYPSCILRGENSVGEFYSVAVTNN
                     YQQADTGTKMIHIGKNTRSTIVSKGISAGHSNSSYRGLVRIAPTAENARNYSQCDSML
                     IGDQCGANTFPYIQVHNNTAVVEHEASTSKIGEDQLFYFAQRGIGAEEAVSMIINGFC
                     KEVFQQLPMEFAVEATKLLSFKLEGSVG"
     gene            complement(2630..2971)
                     /locus_tag="PKZ49_05740"
     CDS             complement(2630..2971)
                     /locus_tag="PKZ49_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HND36098.1"
                     /translation="MAITLTERAAKQICNQLDQQGKGLALKIGLKQSGCSGFGYTFDY
                     VDKIEESDELSITHNAKVLIPKENLSHFDGAELDFVKEGLNSSFRFNNPNVENACGCG
                     ESFGLKISEEN"
     gene            complement(2987..3454)
                     /locus_tag="PKZ49_05745"
     CDS             complement(2987..3454)
                     /locus_tag="PKZ49_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HND36099.1"
                     /translation="MRLTTKGRFAVTALLDLAMQHSNNPVTLADISQRQKISLSYLEQ
                     LFAKLRQRELVDSVRGPGGGYCLAKSLDQVSIADIILAVDEPIDNRRCGGKENCLDEG
                     KCITHDLWAELNDIIFNYLGSVTLKQLIDDQKKREQQQGQSVVKLYDMREAAF"
     gene            3656..3732
                     /locus_tag="PKZ49_05750"
     tRNA            3656..3732
                     /locus_tag="PKZ49_05750"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3690..3692,aa:Met,seq:cat)
     gene            4104..4325
                     /locus_tag="PKZ49_05755"
     CDS             4104..4325
                     /locus_tag="PKZ49_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36100.1"
                     /translation="MENEPINRQSISTEADVDHLITLYKEVCTENTQLKKQLADVLQK
                     HSQLTDKIETAAYRLEKLLSDLPIDKKQD"
     gene            complement(4407..4607)
                     /gene="rpmE"
                     /locus_tag="PKZ49_05760"
     CDS             complement(4407..4607)
                     /gene="rpmE"
                     /locus_tag="PKZ49_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HND36101.1"
                     /translation="MKEGIHPNYQDILVTCSCGNSFTTRSTLCKALHVEVCSACHPFY
                     TGKQKILDTAGRVEKFNRKYAK"
     gene            5058..6923
                     /locus_tag="PKZ49_05765"
     CDS             5058..6923
                     /locus_tag="PKZ49_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HND36102.1"
                     /translation="MNVFYEEAGTFKVGTILAESANSLQVEATHGKRSKIKISAVLLQ
                     FDAPPVYEFMYQAQNIAKDLDINFLWECCNQNTEFTSKTLATEYFGHLPSAVEIAAIM
                     ISLNNAPIYFYKKGQGRYKAAPPEALRAALASQEKKRLDQEKQAHYMQQLNQFILPEA
                     FQKQLDALLYAPDKNTIEWKALEAAAQTTKLSPIKLLEKCGAIPSSHDYHFNQFVREY
                     FPDGVAFDVIQNDPTLDHLKNLTTASEVKAFSIDDATTTEIDDAFSVQPLSLGSFRIG
                     IHIAAPALGILPGSQLDQAAAKRLSTVYLPGKKITMLPDEVIQHFTLVESGYSPALSL
                     YLDVSDDFTVMTKQSKIEMIQVDANLRHDSLEQLFNEETLRRKILDYPFAKELELLWN
                     FAKKMEALRGKENDINNEKIDYSFLIDGERVTIQERRRGSPIDKVVSELMIYTNTEWG
                     KQLAEEGVAAIYRNQSGGKVRMSLSPAPHQGLGVSQYAWSSSPMRRYVDLINQRQLIA
                     LLTGAPPPYTKTSHELMASMHNFEQIYTIYSEFQRAMERYWCLRWLIQEKIQQIEGQI
                     IRENFVKFDHLPLVTRISTLPNLEPGTAVLVEISHIDLLERTLNARFISKINDHA"
     gene            7082..7984
                     /locus_tag="PKZ49_05770"
     CDS             7082..7984
                     /locus_tag="PKZ49_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HND36103.1"
                     /translation="MIDRTLQISSPWFEDQTNRLYVAIAISMAIHIFILMGINFKLPD
                     PPFNNIAKSLEVILVNSKSDQKPTEAKVLAQSNLDGGGNVDENQRAKTPFPVLPQSKP
                     SINLHEATQKTKQLEAEVKRLMTAAKSKPQIPQTTLAQTQTETKQPIQLDSTDLLQRS
                     LEIARLEAQIAKEHEEYQKRPKRKFIGARTREYRFARYVEDWRMKVERIGNLNYPEAA
                     KKEKLYGSLQLTVGIRSDGSLESIGIDRSSGFKILDEAAIRIVRIAAQNGFSAFPPEI
                     SRDTDILHITRTWVFARSDKLHSE"
     gene            8043..8861
                     /gene="aroE"
                     /locus_tag="PKZ49_05775"
     CDS             8043..8861
                     /gene="aroE"
                     /locus_tag="PKZ49_05775"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041352350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HND36104.1"
                     /translation="MTDRYAVIGNPIHHSKSPLIHREFARQTQQDMSYEAILSPLDQF
                     VHTVRSFQNQGGKGLNVTLPFKIEAFRLASQLTERAQSAQAVNTLQFCNNSEILGDNT
                     DGAGLVRDIEVNQGVSIRNKNILLMGAGGAARGVILPLLKLSPNRLTIANRTLDKAIE
                     LQQQFAGFGNIRSTSYPELIQEKFDIIINATSASIANELPPLTPNVFVAQTLAYDMMY
                     GKQLTPFLQFAQCSGVNHLVDGIGMLVEQAAESFYLWRGIRPETTTVIRMLKNS"
     gene            8940..9638
                     /gene="mtgA"
                     /locus_tag="PKZ49_05780"
     CDS             8940..9638
                     /gene="mtgA"
                     /locus_tag="PKZ49_05780"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HND36105.1"
                     /translation="MIKSLLRWVGWLLLLLFSLLLLLQAWFLLHILYWNQYNPSSSAF
                     MQIRWEIARQNQPHVKFHYQWVDYEKISPHLKRAIIAAEDSQFLNHAGFDFKAMQNAL
                     EKNWQEGRWAVGGSTISQQLAKNLFLSDQKTPWRKLQEAVITYMLEKVMAKRRILEIY
                     LNMIEWGENVFGAEAASQHYFGVKASALTPRQAAFLASMVPNPRFYDRNRTAKKLLKK
                     TDIILKRMPSSQIP"
     gene            9828..11261
                     /gene="mgtE"
                     /locus_tag="PKZ49_05785"
     CDS             9828..11261
                     /gene="mgtE"
                     /locus_tag="PKZ49_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HND36106.1"
                     /translation="MMDNFDNIELQNKLERVINLLRKYRLVEGLVQMQDSPRQELIES
                     VTQKQNLASLQKLIDTLHPADIAYILESLPLEDRLFLWKFVKVENDGEVLLEVSDAVR
                     ETLIADMGSNELVAAAEQLDADEIADIAPNLPDDVIEDVYRALPQEEREQLRAAMSYP
                     EDSVGALMDFDIITIREDVRLEVVLRYLRFLGELPDHTDQLFVVDRNELLQGVLMLNQ
                     LLVNDPETMVSNVMTKEGVKFHPHDNAQQATQAFERYDLVSASVVDADGKLLGRVTVN
                     AVIDFIRNKAESEARNLAGLSEQEDLFAPIWKSVRNRWAWLAINLVTAFIASRVIGLF
                     EDSIEKLVALAALMPIVAGVGGNSGNQTITMIVRALALGQVSTENTVKLITKEIGVSL
                     VNGLLWGGIVGLFTFLIYQNASLGLVMALAMILNLLLAALVGVIIPLTRKKLGRDPAI
                     GSSVLITAVTDSGGFFIFLGLATLLLL"
     gene            complement(11341..11790)
                     /locus_tag="PKZ49_05790"
     CDS             complement(11341..11790)
                     /locus_tag="PKZ49_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HND36107.1"
                     /translation="MSIGGICNRKVVVMQREETIAEAAKLMRDQHVGSVLIVDEQDGK
                     RVPVGIVTDRDLVVEVIAPELDADAITVGDIMMTGFAVVKEETGVFEAIQYMRIKGIR
                     RLPVVDAEERLIGIVTLDDLLILLAEELDALAKLVAREQQNEVVVRR"
     gene            11963..>13147
                     /locus_tag="PKZ49_05795"
     CDS             11963..>13147
                     /locus_tag="PKZ49_05795"
                     /inference="COORDINATES: protein motif:HMM:NF020036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HND36108.1"
                     /translation="MMDSIANLSFSSLLDAFSDPLLLIDNHGIIQQTNLATQNIAGYT
                     NDQLQGKSIDQLIPGWYDTIHLYSQRTALKPLGNRSLESSRTKLIKQNKQALLIDIFP
                     KSIEIDHQRFILLTLHAANSQHQAEHNLQESEERLFLAKQAAGLGIFDLDLQQQIIYQ
                     DERMRELWGGKASKISPYDAFYAAIHPKDRISYQIALEKATNPSGDGEFNIDVRVIHS
                     TNGLERWIHSIGKVHFYEGVATRLIGIVQDITQQKLLEKELQSYRAEVESLFKQQVAI
                     QTASAIAHELNQPLTVISAYSELALITLENNPANLDKIKQSLHNCVIQSQRAGDTLRE
                     LIASLQKTDAHIERLDLNQVILDALRTVRSDGFSDFQQILQFELNLPAVLANRVQIKK
                     VVV"
BASE COUNT         4034 a         2910 c         2407 g         3796 t
ORIGIN      
        1 gattatgcga aagaatcaat gaagataaat gcccttgaat aatcttctca ctagaatcaa
       61 tttgagataa ctctgccaca taatgcccat cgataaatac caagcgatat tctgattcac
      121 tctcactcaa aaagaaacgc gctaattcac caagctggtc tcttggaaaa agtaatgctt
      181 cagggaaaga tagcttagcc aacgcagaca gatcggtaaa acgccaagct tccatatgcg
      241 ttgttggcat tcttaattta ttaacacaat gcagcgcctc agagcgaagt tgatttaacc
      301 acgctacatt cgtagaatta gagtaagatc cccagcgttg aacgagacta ttaatgtagt
      361 cattatttga taaagtcatt aaagtcatat gcttgactcg gccgattttg caagttgatc
      421 ggcctttatc caatcatatc cgtgcacttc aagttctttt gctaagtctt ttccactggt
      481 tttgataata cgacccgatt ccataatatg cacataatcg ggcacgatat aatcaagcaa
      541 acgttgataa tgggttacaa gaataatcgc attatcttta ttagtcagct tattcactcc
      601 tgaagctacg attctcaatg catctatatc gagacctgag tctgtttcat ccaatatggc
      661 aagtttcggt tccaatagtg ccatttgcaa tatttcattg cgtttctttt ctccgccaga
      721 gaaaccctca ttaacgctac gatcaagaaa atcaacattc atttccaata gctgcatttt
      781 ttctcgaaca aaatcatcaa actcgagcgg atctaattcc tctttaccac gacttaattg
      841 attcgtgtta taagcgagtc ttaaaaattg actatttgca acacccggta tctcgatagg
      901 atactgaaaa gcaagaaaga taccattccg agagcgaact tctggttcaa gtgaaagtaa
      961 atcttggtta tcaaaggaaa ctgatcccgc ggtaacttga taacctgggt gtccagctaa
     1021 aactttggca aaagtactct taccagaacc atttaacccc ataatggcgt gaatttctcc
     1081 gcattttacc gtgagattca cgccctttag tatttcaaca tcacttactt ttgcatgaag
     1141 atcacgaacc tcaagcaggg ttttactatt ttgatcaatc atcctacact tccctcaagt
     1201 ttgaagctaa gtaacttagt tgcttccaca gcaaattcca tcggtaactg ttgaaatact
     1261 tctttacaga aaccattaat aatcatagaa acagcttcct cagctccaat tccacgctgt
     1321 gcaaagtaaa acagctgatc ctctccaatt ttagaagtag aagcttcatg ctccacaact
     1381 gcagtattgt tatgcacttg gatataagga aaggtatttg caccgcactg gtcaccaatg
     1441 agcattgagt cacattgtga ataattacgt gcattttctg cagttggcgc tattcttacc
     1501 aacccacgat agctactatt cgagtgaccg gcggaaattc ctttactcac gatcgtgctt
     1561 cgcgtatttt ttccaatatg aatcatttta gtgccagtat cagcttgttg atagttattg
     1621 gttaccgcaa ccgaataaaa ttcacccact gaattctcac cacgcaaaat acacgatggg
     1681 tatttccagg taattgctga gcctgtttcg acttgagtcc aagaaatacg ggaattaatt
     1741 cccttagcca aaccacgctt agtcacaaag ttatagatcc cgccaatccc tttttcatct
     1801 cccgcatacc aattctgaac tgtagaatat ttgatttccg cattatccag tgcaaccaac
     1861 tcaacgaccg cagcatgcaa ttgattcgta tcaaagcgtg gcgcagtaca accttctagg
     1921 tacgatactg atgcgccatt ttcagcaata ataagtgttc gttcaaattg acctgaacct
     1981 tcagtattaa tccgaaaata ggtcgaaaga tccatgggac atttaacgcc ttccggaata
     2041 taacaaaatg aaccatcagt aaatacagcg gagttaagtg ctgcataaaa gttatctcct
     2101 actggaacaa cactaccaag atatttctga actagttcag gataattctg aactgcttct
     2161 gaaatcgaac agaaaattat cccaacttct gccaattttt ccttaaacgt cgttgcaact
     2221 gaaacactgt caaaaatcac atcgacagcc acaccagcca atgcagcacg ctcatgtaat
     2281 ggcactccta atttctcaaa agtacggagt aattctggat cgacttcatc catactcgct
     2341 agctttttct taggttttgg cgctgaatag tagctgatat cctgaaaatc aatcttcgga
     2401 tacttaacat tttgccactg tggttcagcc atactcagcc atttctcgta agctcttagt
     2461 cgaaaactaa gcaaccagtc aggctcattt ttcttggctg aaatcatgcg aatgatttct
     2521 tcattaagcc ctttcggagc aatatctgat tcaatttcag taacaaaacc gtgtttataa
     2581 ggttgattta ctaaattttg tagtaaagca ctcattttta atcgtttcct taattttctt
     2641 cagaaatttt taatccaaaa ctttccccac agccacaggc attttcaaca ttaggattat
     2701 tgaatctgaa gctactattt aacccttctt ttacaaaatc gagttcagcc ccgtcaaagt
     2761 gagacaaatt ttcttttggt atcagaactt ttgcattatg agtaatagat agctcatcac
     2821 tttcttcaat cttatcaaca taatcaaagg tataaccaaa tcctgaacaa ccagattgct
     2881 taaggcctat ttttaatgcc aaacctttac cttgttgatc aagttgatta cagatctgct
     2941 ttgccgcacg ctcggttaat gtaatcgcca tagtaaataa aaataattag aaagcagctt
     3001 cacgcatatc atataatttc actactgact gaccttgttg ttgctcacgt ttcttttgat
     3061 cgtcaatcaa ttgcttgagg gtgacgctac caagataatt aaaaataata tcgttgagct
     3121 ctgcccataa gtcatgtgtt atacattttc cttcatctaa gcagttttct ttccctccac
     3181 accgacgatt atcaatcggt tcatcaactg ccaaaataat gtctgcaatc gaaacctgat
     3241 ccaggctttt agctaaacaa tacccacctc ctggtccacg aacactatca accaattcac
     3301 gttgacgtaa tttggcaaat aattgttcca aataagaaag ggaaattttt tggcgctgac
     3361 ttatatctgc aagtgtcact gggttattgg agtgctgcat tgctagatca agtagcgctg
     3421 ttaccgcgaa tcgtccttta gtcgttaatc tcatagaaga tccttatagc atcgttggta
     3481 aattacttga ttatttttgt caactatata ataccttact aagatagtca actataacta
     3541 ataaaaattt atcgtgacga tgtctctaat ctgcccaaat ttaaaaaata aaaccccttt
     3601 ctacttttcc aatttgactg agaatatcat ctactgctat gatcgcactt tctggcgcgg
     3661 ggtggagcag tctggcagct cgtcgggctc ataacccgaa ggtcgtaggt tcaaatcctg
     3721 cccccgcaac cattttgccc tcttgataaa aaattacgtt ataaaaataa caagttaggc
     3781 ttatctatgc agccagctta cagctccgtt ctattcaaga ctttcttaag tagaatcatt
     3841 cgaaaaagct ttgaacttca gaaaaattca actaataatt agtcatcgca tagcttaagt
     3901 atttgactaa agattctctc tgttattaat attgttagcg ctatggtaaa attgaatatc
     3961 ttattaatat tcaatgtcct taaatcagga gatgcgttgt gtatcgcgca aagatttctt
     4021 ggggtctaac cagtaaattg agggaatggc tataacggtt agttaattga ccagagacta
     4081 ttctaaaatt atatttcttc ccaatggaaa atgagccaat caataggcaa tcaatatcaa
     4141 ctgaagctga cgttgatcac ctaattacac tgtacaagga agtctgtaca gaaaatactc
     4201 aactaaaaaa acaattagca gatgttttac agaagcactc ccagctaacc gataaaatcg
     4261 aaactgctgc ttatcgttta gaaaaacttt tgagtgattt acctattgat aaaaaacaag
     4321 actaaacatc acgaaataac aagaaactac aatcgagcca tttaattaaa aataccgact
     4381 actgctcatt taatatttaa tacaatctat tttgcatact tacgattaaa tttctctacc
     4441 cttcccgcag tatcaagaat tttctgtttt ccagtataaa aagggtggca ggctgagcat
     4501 acttcaacat gcaatgcttt acacaatgta gaacgcgtag taaatgaatt gccacaacta
     4561 catgtcacta gtatatcttg ataatttgga tgaatccctt ctttcataat attttatgtc
     4621 cttaaatact cacttgctat tatagacttc atttgttatt caaattttgg cagcacacta
     4681 ttatccttta atcgataact gttagcaaca aattagaaat attcgatttg tagcaaaaaa
     4741 ctatccactt tgagacgcat tatgtatatt taaatcgtta cgcagttgtg gcgaaaatca
     4801 ctcgctctct acttattaaa atcagcaacc tatcgaattt tgcacccccc ccctataaat
     4861 tgatggcaac agaacatcaa tatagttttt tctcaagaaa gcttagaccg cttgcgtacc
     4921 agtgataaat cattctgcat ttctgcatta ataaaatatt gaaaagactt tatcaaaact
     4981 ttcagaaaaa tatcgcaaca ttaggctatg attgacttgt tttcaatttt ttatcgcttt
     5041 tctgaaaggt cccatcagtg aatgtgtttt atgaagaagc aggtacgttt aaagttggca
     5101 cgattctagc tgaaagcgct aattctttac aagttgaagc aactcacggt aaacgctcaa
     5161 aaattaaaat ttccgctgtt ttgcttcaat ttgatgcgcc acctgtgtac gaattcatgt
     5221 atcaagcgca gaatattgcc aaagatctgg acataaattt cctttgggaa tgttgtaatc
     5281 aaaacacaga attcacgagc aaaacccttg ccaccgaata ttttggccat ctccctagtg
     5341 cagtagaaat tgctgccatt atgatctcat taaataatgc accgatttat ttctacaaaa
     5401 aaggacaggg gcgatataaa gcagcacctc cggaagcgct tagagccgcg ctagccagtc
     5461 aggaaaaaaa acgcttagat caagaaaagc aagcgcatta catgcagcag cttaatcaat
     5521 tcatccttcc tgaagcattc cagaagcagt tagatgccct gctctatgcg cccgataaaa
     5581 atacaattga atggaaagcg ctcgaagctg cagcccaaac tactaaactc agccccatca
     5641 aattgctgga aaaatgtgga gcaatccctt cttctcatga ttatcatttt aatcaatttg
     5701 tacgtgaata ttttccagat ggcgttgcat tcgatgtaat acaaaacgat cctacgcttg
     5761 atcacctaaa aaatcttaca actgcgagtg aagtcaaagc atttagcatt gatgatgcca
     5821 cgaccacaga aattgacgat gcattctcag ttcaacccct gtcgctggga agttttagaa
     5881 ttggtatcca catagcagcc cctgcattag gaatattacc tggatctcag ctcgatcaag
     5941 ctgctgcaaa gcggctttct acggtttatt tgccagggaa gaaaatcact atgctgcctg
     6001 atgaggtgat tcaacatttt accttggtag aatcaggtta tagccctgca ttatcgctat
     6061 atctcgatgt ttctgatgat ttcaccgtga tgaccaagca aagcaaaata gaaatgattc
     6121 aggttgatgc gaatcttcgt cacgattctt tagaacagct attcaatgaa gaaacactaa
     6181 gacgaaaaat ccttgattat ccttttgcta aagaactcga gttactctgg aattttgcta
     6241 aaaaaatgga agcgctgcgg ggcaaggaaa atgatattaa caatgaaaaa attgattata
     6301 gcttcttgat cgacggcgaa cgagtcacaa tccaagaaag acgccgtggt tctcctatcg
     6361 ataaagttgt ttctgaattg atgatttata ctaacaccga atggggtaag caactcgccg
     6421 aagaaggtgt tgccgcaatt tatcgcaatc aaagcggtgg aaaagtcaga atgagccttt
     6481 ctccagcccc tcatcaaggc ttaggtgttt cacaatacgc gtggagtagc tcacccatgc
     6541 gacgctatgt tgatctaatc aatcagcgac aactcatcgc cttactgacc ggagctcctc
     6601 ccccttatac caaaacgagc catgaactga tggcctccat gcataatttt gaacaaattt
     6661 atacgattta tagtgaattt caacgcgcaa tggaacgcta ctggtgcctt cgttggttga
     6721 ttcaagaaaa aatccagcaa attgaaggcc agattattag agaaaatttt gtcaaattcg
     6781 atcatttgcc attagtcacc cgaatatcga cactccccaa tctagagcct ggtacagcag
     6841 tgctggttga aatttcacat atcgacctac tggaacgcac gttaaatgca cgatttatca
     6901 gtaagataaa cgatcatgct tagtattttg taaatagcaa aaaaatggta tagatttcgt
     6961 ctatctgcac taaatatttt caagtatcat gctggagctc acggtcaaaa tagcatatgc
     7021 actaactaag caatattagt acgattcaaa ggttatattt accccccctg tttatttgat
     7081 aatgatagat cgcacacttc agatatcgag tccttggttt gaggatcaaa caaaccgctt
     7141 gtatgtcgcc attgcgattt cgatggccat acatatcttt attttaatgg gtattaattt
     7201 taagctacct gatcctcctt tcaataatat tgccaaatca ttggaagtca ttcttgtcaa
     7261 cagcaagtct gatcaaaaac caactgaagc aaaagtgctc gcacaaagca atttagatgg
     7321 gggaggcaat gttgatgaaa atcaacgcgc caaaacaccg tttccagttc ttccccaatc
     7381 aaaaccatct attaatcttc atgaagcaac tcagaaaaca aagcagctag aagcagaagt
     7441 taaacgactc atgactgctg caaagagtaa accacaaatt ccccaaacca cactcgcgca
     7501 aactcaaact gaaacaaagc aaccaataca gctggatagt accgatttac tacagcgcag
     7561 ccttgaaatt gcacgcctgg aagctcaaat cgcgaaagag catgaagaat accaaaagcg
     7621 gcctaagcgc aaattcattg gcgcgcgtac ccgcgaatac cgatttgcac gttacgttga
     7681 agattggcgt atgaaagtcg agcgtatcgg aaatctaaat tacccagagg ctgccaaaaa
     7741 agagaagctt tatggaagtt tacagttaac cgtcggtatt cgctctgacg gaagtcttga
     7801 atccattgga atcgatcgct catcgggttt caagattctt gatgaagcag caattcgaat
     7861 tgtccggatc gcggcacaaa atggtttttc agcttttcca ccagagatca gccgtgatac
     7921 cgatatcctg catataaccc gtacctgggt gtttgcgcgt tccgataagt tacatagtga
     7981 gtaaatttct gcaatggtaa ccaattagcc aatcgatatt actttgagtc tctacttaga
     8041 ccatgactga tcgatatgct gtaattggca accccatcca ccatagtaaa tctccactta
     8101 ttcatcgcga gtttgcccga caaacgcaac aagatatgag ctacgaagcg atcctttccc
     8161 cgttggatca atttgtgcat actgttcgat cattccaaaa tcaaggtggc aaaggactca
     8221 atgtcacatt accctttaaa atagaggctt ttaggctagc atcacaactc actgagcggg
     8281 ctcaatccgc tcaagctgtt aatacccttc aattttgtaa taatagtgaa attcttggcg
     8341 ataacaccga tggtgctggg ttagtacgtg atatcgaagt caaccaaggt gtttcgatta
     8401 ggaacaaaaa tattttgtta atgggggcag gaggagcagc tcggggtgtt atcctaccac
     8461 tcctcaagct tagtcccaat cgactcacca ttgcaaaccg aacccttgat aaagcaattg
     8521 agctgcaaca gcagtttgct ggctttggta atattcgatc gacttcctac ccagaactga
     8581 ttcaagaaaa atttgatatt atcatcaatg caacatcagc cagtatcgcc aatgagctac
     8641 ctccgttaac acccaatgtt tttgtagcac aaaccttagc ttacgacatg atgtatggaa
     8701 aacaattaac cccatttctg caatttgcac agtgtagtgg tgtcaaccac ttagttgatg
     8761 gaattggaat gctcgttgag caagctgcag aatcgtttta tttatggcga ggcataagac
     8821 ctgaaacaac aacagtgata cgtatgctta aaaattccta atacgtatcg atctcataaa
     8881 actgattcgg ttatctacct acctatagtc gctgatccac caaataccgc cgtaactaaa
     8941 tgatcaaatc attgctgcga tgggtaggat ggttgttact cctacttttt agtcttttat
     9001 tattgctcca agcgtggttt ctgctgcaca tcctctactg gaaccagtat aatccttcgt
     9061 ccagcgcttt tatgcagatt cgatgggaaa tagcacgcca aaaccaaccc catgtgaaat
     9121 ttcattacca atgggttgat tatgaaaaaa tttctcccca tctcaagcgc gcaattattg
     9181 cggccgaaga tagtcaattt ttgaatcatg cgggttttga cttcaaggca atgcaaaacg
     9241 ccttagaaaa aaactggcaa gaaggtcggt gggcggttgg cggttcaact atcagtcagc
     9301 agcttgcaaa gaatttgttt ctttctgatc agaaaacacc ttggcgtaag ctacaagaag
     9361 cggtcattac ttacatgtta gaaaaagtaa tggccaagag gcgcatctta gaaatttatt
     9421 tgaatatgat cgaatggggt gaaaatgtat ttggtgctga agcagcttcc cagcattatt
     9481 ttggggttaa ggcttcggca ttaactccca gacaggctgc cttccttgca tccatggtcc
     9541 ctaatccacg tttttatgat cgtaatcgca ctgcaaaaaa actgttaaaa aaaacggata
     9601 tcatcttaaa acgtatgcct tcttctcaga tcccctaaaa aattacttaa tactcttttt
     9661 ccacacaatt gaatagcctt gattttgtta tcttctaatc ttatcttcta atcttatctt
     9721 cgatacgatg caacatgtta aattagagaa tttcaggcta agcaactttt taatcatcca
     9781 atccactcaa tcaggagtag caacttcttc aataggcatg gcatgagatg atggataatt
     9841 ttgacaatat agagctacaa aacaagcttg agcgcgtcat caacttactg cgtaagtacc
     9901 gactggtcga aggcttggtg caaatgcaag actctccacg ccaagaattg attgagtcgg
     9961 tcacgcagaa acaaaacctg gcaagcctac aaaaattaat cgataccctg catcctgctg
    10021 atatcgctta tattttagaa agcctgccac tggaagatcg gttattcctt tggaaatttg
    10081 ttaaagtcga gaatgatggc gaagtactcc tcgaagtatc cgatgccgta agagaaacgc
    10141 taattgctga tatgggaagc aacgaattgg ttgcggccgc cgagcaatta gacgccgacg
    10201 aaattgccga tatcgcaccc aatttaccgg atgacgtcat tgaggatgtt tatcgcgcac
    10261 ttcctcagga agagcgtgag caactccgtg cagcgatgtc ctatccagaa gattctgttg
    10321 gagcactcat ggatttcgat atcatcacga tccgtgaaga tgttcgatta gaagtcgtgt
    10381 tacgttacct acgctttctc ggtgaattac ccgatcatac cgatcagtta ttcgtcgtcg
    10441 atcggaatga attgctacag ggcgttctca tgctcaatca attgttagtc aatgatcccg
    10501 aaacgatggt cagtaatgtc atgactaaag aaggtgtaaa atttcacccc catgataatg
    10561 cgcagcaagc cacgcaagca tttgaacgct atgatttggt ttcggcttcc gtcgtcgacg
    10621 cagatggaaa actgttgggc cgtgttactg ttaatgctgt gatcgatttc attcgaaata
    10681 aagcagagag tgaagcgcgc aatcttgcgg gattgagcga gcaagaggat ttatttgccc
    10741 ccatttggaa aagtgtgcgt aatcgatggg cgtggctcgc aattaatttg gttactgcat
    10801 ttattgcatc gcgcgtcatt ggcctatttg aagattcaat cgagaagcta gtcgcattag
    10861 ccgccttaat gccgattgtg gctggcgttg ggggtaactc cggtaatcaa accatcacga
    10921 tgatcgtgcg tgcgttagcg ctaggtcaag tcagtactga gaataccgtc aaactcatca
    10981 ccaaagaaat tggggtcagc ttggtaaatg gccttctatg gggaggaatc gtcggtttat
    11041 ttacgtttct gatttatcag aatgcctccc ttggtctcgt tatggcacta gcgatgatat
    11101 tgaatctttt actcgcggca ctagtcggcg tcatcattcc gctgactcgc aaaaaattag
    11161 gccgtgaccc tgcaatcggt tccagcgttt taattactgc tgttaccgac agcggaggat
    11221 tcttcatctt tctagggcta gcaacactgc tacttttatg atttgatatt ggcgctttgg
    11281 cttaaagaga tccaagaatg gtttgtacag caacagagct agctgctaca gtccgtcttc
    11341 tcaacgccga acgaccactt cattttgctg ctcacgtgca accaatttgg caagcgcatc
    11401 caattcctct gccaataaaa ttagcaaatc atccaacgtc acgatgccaa ttaacctctc
    11461 ttctgcatcc actaccggta agcggcgtat cccttttatc cgcatatatt gaattgcttc
    11521 aaaaacccca gtctcttcct tgaccaccgc aaaaccggtc atcatgatat ctccaaccgt
    11581 aattgcatct gcatcgagtt ctggtgcaat tacttcaacc actagatcac ggtcagtcac
    11641 gataccaacg ggaacacgct tcccatcttg ctcatcaacg attaaaacac tccccacatg
    11701 ctgatcacgc atcaatttag cggcttccgc aattgtttcc tcacgttgca tcaccactac
    11761 tttgcgatta caaatccctc caattgacat tcactatcct ctctttttaa atactaatct
    11821 ctattatttt caatatagcg aaaacagagc atgatataaa tacgtagcat cacttagggt
    11881 taaactttcc ctatattgga gtttgcatcg acacgattcg ctgccttcca taacaacttt
    11941 caaatcaaaa catgaaagaa taatgatgga ttctattgct aaccttagtt tttcctcatt
    12001 gctggatgct ttctccgatc cattactgct catcgataat catggaatta tccaacagac
    12061 caatctagct actcagaaca tagcaggtta cacaaatgat caactccaag ggaaatcaat
    12121 cgatcaactg atacctggct ggtacgatac aattcacctt tatagccaaa gaactgcttt
    12181 aaaaccatta gggaatcgat ccttagaaag tagtcgtact aagctaataa aacaaaacaa
    12241 gcaggcgtta ctgatagata tcttcccaaa atcgattgaa atcgatcatc agcggtttat
    12301 tcttctcact ctccatgcag ctaactcgca acaccaagct gagcataatt tgcaagaaag
    12361 cgaagagcga ttgtttctgg ccaagcaagc ggcaggactt ggtatttttg atctggatct
    12421 acaacaacaa attatttatc aagatgagcg tatgcgcgaa ctctgggggg gaaaagctag
    12481 caaaatctct ccatacgacg cattttatgc ggctattcat ccgaaagacc gtatctcata
    12541 tcaaatcgct ttagaaaaag caacgaatcc gagtggtgat ggtgaattca atatcgacgt
    12601 acgagtcatc cattcgacaa acggtttaga acgatggatt cactccattg ggaaagtaca
    12661 tttctatgaa ggggtagcga ctcgtctcat tggcatcgta caagatatca cgcagcaaaa
    12721 attgctagaa aaagagctgc aaagctatcg tgctgaagta gaatcactat ttaaacagca
    12781 agtcgcaatc caaaccgctt ctgcgattgc ccatgagctt aaccaaccgc ttacggtaat
    12841 ctctgcttat agtgaattag ccctgattac attagaaaat aacccggcta atctggacaa
    12901 aatcaagcaa tcactccaca attgcgtcat tcaaagtcag cgggcgggtg atacactgcg
    12961 tgaattgatt gcatctttac aaaaaaccga tgctcatatt gaacgactgg atctcaatca
    13021 agttattctc gatgcgctgc gcacggtgcg cagcgatggg tttagtgatt ttcagcaaat
    13081 cctgcagttc gaactcaatc ttcctgccgt tttagccaat cgtgtccaaa tcaagaaagt
    13141 cgtggtt
//
LOCUS       DAOQKQ010000065        12993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_2247_length_12993_cov_6.109290, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000065 DAOQKQ010000000
VERSION     DAOQKQ010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 12993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12993
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2247_length_12993_cov_6.109290"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /gene="hemW"
                     /locus_tag="PKZ49_05800"
     CDS             <1..982
                     /gene="hemW"
                     /locus_tag="PKZ49_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HND36109.1"
                     /translation="SISSIFIGGGTPSLLSASGLEQLLSQVRMLLLLEPSVEITLEAN
                     PGTFESKKFEAFREAGVNRLSIGVQSFNTEQLLKLGRIHNANEARHAIEISQHYFDNI
                     NLDLMYALPNQTIEEARVDIETACTTGVPHISAYHLTLEPNTMFYQYPPQLPDDDLSA
                     DMQALIEQTLKSSGFAQYEISAFAHAGHRSHHNLNYWQFGDYLGIGAGAHSKISFRDK
                     IVRQMRYKHPKKYLEVLLSQSVTPIQMEQELEVADRIFEFMMNSLRLIEGFRLEDFRS
                     RTGLPIIAAQQQLKVAEQMGLIHRDCHRIVPTDIGRKFLNDLLQIFLPNK"
     gene            1022..1816
                     /locus_tag="PKZ49_05805"
     CDS             1022..1816
                     /locus_tag="PKZ49_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HND36110.1"
                     /translation="MTYVKLTEVVECMLDAGTKKANLSIKDMLIRGALAGAFLGYATS
                     LAVLVIVQTGLGIWGAIVFPVGLVMIVLLGLELATGNFAVIPIAVKDGRTRIGRLFHN
                     WFWVLVGNLIGGVGYAFLFVLVITKLGHVDPINLDQAIKLVALAEQKTIAYAAIGFGG
                     MATAFISAMLCNWMVALGSVIAFTSTSTGGKIAAMWLPTMTFFALGYEHAIVNMFVIP
                     AGMMLGANITFLEWWTWNGIPVILGNILGGMVFTGLALYITTRGQR"
     gene            complement(1839..2240)
                     /locus_tag="PKZ49_05810"
     CDS             complement(1839..2240)
                     /locus_tag="PKZ49_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36111.1"
                     /translation="MLVKYNLNHQAVTYYWKPISRYQQFQLVGEFYYQSAFKKLVENH
                     GNRGVYFECLATLIPENTNAFDSQAIRIDIKGMTVGYFNRNDARKFRIRLNTNKLAGQ
                     ITSCNAVIEGGRLLNDGRRSNYGVSLAINQL"
     gene            complement(2546..2866)
                     /locus_tag="PKZ49_05815"
     CDS             complement(2546..2866)
                     /locus_tag="PKZ49_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HND36112.1"
                     /translation="MLLTTTPMIEGKRITQYCGIVAGEAVLGANVLKDLFAGIRDFIG
                     GRSGTYEKELQRARDIALNELKDKAKELGANAVIGIDIDYEVLGKENGMLMVSASGTA
                     VIFE"
     gene            3047..3424
                     /locus_tag="PKZ49_05820"
     CDS             3047..3424
                     /locus_tag="PKZ49_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HND36113.1"
                     /translation="MSWIRNEPLRRVVFWQTLATGLATLVCGWLMGMHGAISALLGGL
                     VSIAASLAYAVLIDRHGGYTAGDVVKTALKAEAIKISIMIVLLLGVFLLYKEVVPIVF
                     IGVFIVAAIIFSMALFVTTAKNK"
     gene            3447..4250
                     /gene="atpB"
                     /locus_tag="PKZ49_05825"
     CDS             3447..4250
                     /gene="atpB"
                     /locus_tag="PKZ49_05825"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HND36114.1"
                     /translation="MAAELTPTSYINHHLTNMTTTVGDGSFWTLHLDTLVMSVMLGIM
                     GMGFLWLIVRKATPGVPSKTQAFVELLVEFIDNQVKNTFHGNRHVFIAPAALTIFVWV
                     LLLNAMDLLPIDIMAWVYEHVFGLHNWRSVPTTDVNTTFALALSIWLLTIFFSIKVKG
                     LGGWMHELFCTPFGTNIILWPLNLLFNMIEYISKPLSHSLRLFGNIYAGEIIFLLLGM
                     WAATGVSGAFFSAILGAGWAIFHILIVSLQAFIFMMLTVVYLAMAHESH"
     gene            4306..4578
                     /gene="atpE"
                     /locus_tag="PKZ49_05830"
     CDS             4306..4578
                     /gene="atpE"
                     /locus_tag="PKZ49_05830"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HND36115.1"
                     /translation="MENLQYLAMIQAYTGIGIGLMIGLGAAGACIGVGVMCSRFLEGA
                     ARQPEMIPTLQGKVFLLLGLTDASFIIAVGLAMLFAFGNPLLAVVQ"
     gene            4614..5087
                     /locus_tag="PKZ49_05835"
     CDS             4614..5087
                     /locus_tag="PKZ49_05835"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HND36116.1"
                     /translation="MNINFTLISQAIAFSVFVWFTVKFVWPPLLRAIEERQKQIADGL
                     AAGERGRKELELADQRSSDVLKDAKQRAADIVTQAEKRAAEIIDEAKSVAKVEGDRII
                     AGAKAEIAHEIFSAKEELRQQVSGLVLTGATKILRREIDAKAHADLLTAIETELK"
     gene            5087..5623
                     /locus_tag="PKZ49_05840"
     CDS             5087..5623
                     /locus_tag="PKZ49_05840"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HND36117.1"
                     /translation="MAESITIARPYAEAVFKLAKEKNSLSHWSEMLSNAAQITEVAPV
                     QELIGNPNVSASKLKEIIFNIGGSKLDGEVKNLLSILIDNDRVTMIPRLVEMYEQLRA
                     NHEGVLEANIISAFSMSDRQLEKLVAALENKFNRKVKAKVSIDQELIGGVKIEIGDEV
                     IDSSISGKLEAMSIALKS"
     gene            5635..7176
                     /gene="atpA"
                     /locus_tag="PKZ49_05845"
     CDS             5635..7176
                     /gene="atpA"
                     /locus_tag="PKZ49_05845"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HND36118.1"
                     /translation="MQLNPSEISELIKSKIEGLSASSEIRTQGTIVSLTDGIVRIHGL
                     SDVMQGEMLEFPGNTYGLALNLERDSVGAVILGSYEHLTEGDTVKCTGRILEVPVGEG
                     LLGRVVNALGQPIDGKGPISAQGKEPIEKIAPGVIWRKSVDQPVQTGLKAIDAMVPVG
                     RGQRELIIGDRQTGKTAVAIDAIINQKGENMICIYVAIGQKASSIANVVRKLEEHGAM
                     AYTIVVVASASESAAMQFIAPYSGCTMGEYFRDRGQDALIVYDDLTKQAWAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVSAEYVEKYTNGAVKGKTGSLTALPIIETQA
                     GDVTAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFAQFASDLDEATRKQLERGKMATELMKQPQFATQSVSEMALTLFA
                     LNKGYLDDVEVKRALAFESALRSYIRNQHAALIDKIESTKNLDEDTEKALIQAIEEFK
                     KNGTY"
     gene            7176..8063
                     /gene="atpG"
                     /locus_tag="PKZ49_05850"
     CDS             7176..8063
                     /gene="atpG"
                     /locus_tag="PKZ49_05850"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HND36119.1"
                     /translation="MAGSREIRNKIKSVKNTQKITRAMEMVAASKMRKAQERMRKARP
                     YGEKIRNVAAHMSRASTEYKHPFLIERDTVKRVGVIVVTSDKGLCGGLNTNILRRALN
                     EMKRWQAEGEEIEVCCIGNKGLGFMNRIGAKVISHVVSLGDTANIEKLIGAIKIVLDG
                     YTNDRFDRVYVFYNRFVNTMKQEPTMELLLPLSDDRFGNNKGDQETSPKKLWDYIYEP
                     EAKSVIDDVMIRYVEALIYQAVTENMASEQSARMVAMKAASDNAGNLINELTLIYNKS
                     RQAAITKELSEIVGGAAAV"
     gene            8096..9475
                     /gene="atpD"
                     /locus_tag="PKZ49_05855"
     CDS             8096..9475
                     /gene="atpD"
                     /locus_tag="PKZ49_05855"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HND36120.1"
                     /translation="MSEGRIVQCIGAVIDVEFQKDGIPNVYDALTMEGSELTLEVQQQ
                     LGDGVVRTIALGSSDGLRRGMMVVNTKKQISVPVGTKTLGRIMDVLGRPIDEMGPVGA
                     EKTMPIHRVAPAFDELSASTELLETGIKVIDLICPFAKGGKVGLFGGAGVGKTVNMME
                     LIRNIAIEHSGYSVFAGVGERTREGNDFYHEMKDSNVLDKVALVYGQMNEPPGNRLRV
                     ALTGLTMAESFRDEGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEMGR
                     LQERITSSKTGSITSIQAVYVPADDLTDPSPATTFGHLDATVVLSRDIASLGIYPAVD
                     PLDSTSRQLDPLVVGEDHYQTAREVQQTLQRYKELRDIIAILGMDELSPEDKLSVSRA
                     RKIQRFLSQPFFVAEVFTGSPGKYVPLKETIKGFKGIVNGDYDDIPEQAFYMVGGIEE
                     VIEKAKKLQ"
     gene            9499..9927
                     /locus_tag="PKZ49_05860"
     CDS             9499..9927
                     /locus_tag="PKZ49_05860"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HND36121.1"
                     /translation="MGTVFHLDIVSAEDSIYSGPAEFLVAPAMLGEVGIFPQHTPLLT
                     RIKSGMVRIKAPLKDEESVYVSGGMLEVQPDVVTILADTAIRSHDLDEAKALEAKKHA
                     EEIMKDKQSGIEYARAQAELIEASAQLAAIQKLRKRGGGH"
     gene            10178..11506
                     /locus_tag="PKZ49_05865"
     CDS             10178..11506
                     /locus_tag="PKZ49_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HND36122.1"
                     /translation="MAKRTKKIYVLDTSVLLFDHNAIKNFEENDVAIPITCLEELDRF
                     KVGNDIKNFEAREVIRFLDKLSEDYGLEKWIPLGEEKGRLRIVMSNSLNGIDAEEIYG
                     KGKNDHKIINAALTLANSHKSARVVLVTKDINLRLKAKAIGLPSEDYETGKVKNVKSI
                     SAGFPILEQIPSSTINEIARRGSIEEAGLLGTHKLANGYYILRNANDSLLARYNQTQN
                     TIERVEKAYVYSIKPRNAEQAFAVNALLNDDIKLVALQGVAGTGKTLLALASALELKN
                     KYDQIILARPIIPLSNRDIGYLPGTAEDKISPFMQPLWDNLKFIKSQFRPTERKSKIL
                     DEMEESGKLAITALAFIRGRSLSNVMFIIDEAQNLTPHEIKTIITRAGESTKIVFTGD
                     IHQIDTPYLDEQSNGLTYIIDRLKGHPLFAHIRLEKGERSELANLANDLL"
     gene            complement(11765..>12993)
                     /locus_tag="PKZ49_05870"
     CDS             complement(11765..>12993)
                     /locus_tag="PKZ49_05870"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HND36123.1"
                     /translation="GLQNPISDAADPELHSKFNAWRESAPNGQLLVLRVRPAELPHLS
                     RYFEPKDVVLHIIDTTTNYIEQFNSTDQVGVADFARVYLGERDQTYCDLEPWAYQLMP
                     AALEKSSTSPNSWQEPFNSTLDKIARYLTFKEVGIAMSSGAALGFAHLGVLDAMAQCG
                     INYDVLCGSSMGGIVALTVAHLGSIDQAIQQVREQLGSNRKIKDPSLLPRGSLFIGNK
                     IAAAAKATFGDCTFADLKHPAAVVAADLVTHERIIFDRGLVAPAVLATSAIPGFFPPV
                     ANQSRLMVDGSVVSLVPVDVLSNRRCGLRIAINVNTKPFASGNDVITGFDGLLQKTQK
                     PLGLKDVLSASWGLLGSWRSSSEALRAEIVINPQTPVESGYNFNEFEKLVDCGRAAMH
                     DRIESIQESIKLMLRA"
BASE COUNT         3751 a         2443 c         3057 g         3742 t
ORIGIN      
        1 atctattagt agtattttta ttgggggagg tacgcctagc ttattgagtg catcaggact
       61 tgagcaacta ttgtctcaag tcaggatgct attgctattg gaaccttccg ttgaaattac
      121 cttagaggcc aatccaggta catttgaatc aaaaaaattc gaagcattcc gtgaagctgg
      181 cgtgaatcgt ctttcaatag gcgttcaaag ctttaacaca gagcagttgt taaagttagg
      241 tagaatccat aatgccaatg aagcccgcca tgcaatcgag atttcacagc attattttga
      301 taatatcaat ttagatttaa tgtacgcatt gcccaatcaa acgattgaag aagcgcgagt
      361 agatatcgaa accgcttgta ctactggcgt gccccatatt tcggcttatc atttaacgtt
      421 agaaccgaat acaatgtttt atcaatatcc accgcaattg ccagatgatg atttgtctgc
      481 tgacatgcaa gcattgatcg agcaaacttt gaagagttct gggtttgctc agtatgaaat
      541 ctcagctttt gcgcatgctg gacatcggtc tcatcataac ttgaactatt ggcagttcgg
      601 tgattatttg gggataggcg cgggcgcgca cagtaagatt agctttcgtg acaagatcgt
      661 tcgccaaatg cgctataagc accccaagaa atatcttgag gtattgttga gccaatcagt
      721 tacgcctatt caaatggagc aggagctcga agtagctgat cgtatcttcg aatttatgat
      781 gaattcacta cgccttatcg agggatttcg tctagaagat tttcgttctc gaacaggatt
      841 acccattatt gctgcacaac agcagcttaa agttgcagag cagatgggat tgattcatcg
      901 ggattgtcat cgtatcgtgc cgaccgatat aggcagaaaa tttttaaatg atctattgca
      961 gatttttttg cccaataaat agcttatcag tatcgttctc agaaagcgtg attgaaatcc
     1021 aatgacttat gtaaaattaa ccgaagttgt tgaatgtatg ttggatgcag gaaccaagaa
     1081 agcaaatctt tcgatcaaag atatgctgat tcgaggggct cttgccggag catttctagg
     1141 ctatgcaaca agtcttgcgg ttttggttat tgttcaaaca ggcctgggaa tttggggagc
     1201 aatcgtgttt cccgtagggt tggtgatgat cgtactacta gggctcgaat tagcaacagg
     1261 aaattttgca gtgatcccga ttgcggtaaa agatggccga actcgtatag gacgtttgtt
     1321 tcataattgg ttttgggttt tagtcggaaa tttgatcggg ggagtaggtt atgcatttct
     1381 ttttgttttg gtcatcacga aactcggtca tgttgatcca attaatctag atcaagcaat
     1441 aaagttagtt gcactcgcag agcagaagac aattgcctac gcggcaattg gttttggggg
     1501 aatggctaca gcctttatca gcgcaatgct atgtaactgg atggtagcgt tagggtcagt
     1561 tatcgctttt acatctacct caacaggagg aaaaattgct gcaatgtggt tgccgaccat
     1621 gacttttttt gcgttgggtt atgagcatgc cattgtgaat atgtttgtta ttcctgcggg
     1681 tatgatgttg ggtgcaaata taacgttcct ggaatggtgg acgtggaatg gtattccagt
     1741 gattttaggt aatattctcg gtggaatggt tttcacaggc cttgcgctct atataacaac
     1801 aaggggacaa agataaggta acgcgttctg attttttgct aaagctggtt aatagcaagc
     1861 gatacgccat aattactacg gcgcccgtca tttaagagac gcccaccttc tatcactgcg
     1921 ttacagcttg taatttgccc agcgagttta ttagtattta atcgtatgcg gaactttcta
     1981 gcgtcattac gattaaaata accaacagtc atccccttaa tatcaatacg aatggcttga
     2041 ctatcaaatg cgttggtgtt ttctggaatg agcgtggcga ggcattcgaa ataaacccct
     2101 ctattgccat gattttcaac tagcttctta aaagcggact gataataaaa ttctccgact
     2161 aactgaaatt gttggtagcg tgaaataggc ttccaataat atgtgactgc ttgatggttt
     2221 aaattgtatt taactaacat gttctatcga tagcctaatc taaaaataaa aatgaattgc
     2281 cttgctgatg taatgaatct gtatccatcg gttacttaag aaacatggga tgttgaattc
     2341 tttacgccaa gaaggtttta gtagctatag agagcaacaa taatgccagt tatttattgt
     2401 gattattaat cagagggtta tgtgtttatt ggtttaatga tggtaagctg gctgtaaaat
     2461 aaattgacgc aatatcactg aatgcatcaa aaaagttgaa tttttctgag aaagatagat
     2521 agaaagcggt tatgttaata ctggctcatt cgaagataac agctgtgcca ctagcagata
     2581 ccatcaacat gccattttct ttgcctaata cttcatagtc tatgtcgata ccgatgactg
     2641 cgtttgcgcc cagttcttta gccttatctt ttaattcatt aagagcgata tcgcgggcgc
     2701 gctgtagttc tttttcatag gttcctgatc gtccaccgat gaaatcacga ataccagcaa
     2761 aaagatcttt cagaacattg gcaccgagca cagcttctcc ggcgacaata ccgcagtatt
     2821 gtgtgatacg tttgccttca atcatcggtg tggttgttag tagcatgatt aatttaaatc
     2881 ttgagattat tatttatgtt ttctacatct ttattcctag tatatttcga gggcatttgt
     2941 cgagtcaaag tcatttgctt cgttgacgta aattgccagg caggttaaaa tctgggactt
     3001 taaagtaaag tgtatttacg tactttctgg ttctggcaaa gattatatgt cttggatacg
     3061 gaatgaacca ttacgtaggg ttgtattttg gcaaacgcta gcaacgggtt tggcaactct
     3121 tgtttgtgga tggttaatgg gaatgcatgg cgcaatttct gctttactgg gcggtttagt
     3181 cagtattgct gcttcattgg catatgctgt attgatcgat cgtcatggag gatatacagc
     3241 cggtgatgta gtgaagacgg cgcttaaagc agaagcgatt aagatttcga taatgatcgt
     3301 actccttttg ggagtatttt tgttatataa agaagttgtt ccaattgtgt ttattggggt
     3361 attcattgtc gcggcaatta tcttcagtat ggctctattt gtaacaactg caaaaaataa
     3421 ataatgggaa taaaaagcta aaaataatgg cagcagaact tacaccaact tcttatatta
     3481 atcaccatct aacaaatatg accaccactg tgggtgatgg ttcattttgg acgctgcatc
     3541 ttgatacgct agttatgtcg gtgatgttgg gcataatggg tatgggtttt ctttggttga
     3601 ttgtgcgtaa ggcgacgccg ggtgtgccca gtaaaacgca agcttttgtt gagttattgg
     3661 tcgagtttat tgataatcag gttaaaaata ctttccacgg aaatagacat gtatttatag
     3721 cgccggcagc cctgacaatt tttgtttggg ttttgctact taatgcgatg gatttgttgc
     3781 ccattgacat aatggcttgg gtttatgagc atgtgtttgg cttacataat tggcgtagtg
     3841 tgcctacaac tgatgttaat acaacatttg cgctagcgtt atcgatatgg ctgttgacga
     3901 tattttttag cattaaagta aaagggttag gtggttggat gcatgaattg ttctgcacgc
     3961 cctttggtac aaatatcatc ttatggccac taaatttact ctttaacatg attgagtata
     4021 tatctaagcc gctttctcat tcattgcgat tatttggaaa tatctatgct ggtgaaatta
     4081 ttttcttatt gttagggatg tgggcggcta ctggtgtatc aggcgcattc tttagtgcaa
     4141 ttttaggggc tggttgggcg atttttcata ttttaatcgt tagtctccag gcctttatat
     4201 ttatgatgct aaccgttgtt tatcttgcaa tggcgcatga gtcccattaa ggttgttgtt
     4261 ttagttagaa atttagtttt attcattaaa taaaaaggaa atgatatgga aaatttgcag
     4321 tatctcgcga tgattcaagc ttacaccgga attggcattg ggttaatgat tggattgggt
     4381 gcggcgggag cgtgtattgg tgtgggtgtg atgtgtagtc gttttttaga gggggcggcg
     4441 cgtcaaccgg aaatgattcc aactttgcaa ggtaaagtat tcttgttatt ggggctgacc
     4501 gatgcttcat tcattattgc agttggtttg gcaatgctat ttgcttttgg taatcctttg
     4561 ttagctgtcg ttcaatagta tttaaacaag aaaaatttta acaaagctgc ccaatgaata
     4621 taaattttac acttatttca caggcgattg ccttctccgt atttgtttgg tttacagtta
     4681 agtttgtgtg gccgccgcta ctgcgtgcga ttgaagaacg tcaaaaacaa atagcggatg
     4741 gacttgctgc tggagagcgt ggaagaaaag aacttgaact tgcagatcaa cgatcatctg
     4801 atgttcttaa agatgcaaag caacgtgcgg ctgatattgt cactcaggca gaaaaacgag
     4861 cggctgaaat cattgatgaa gcaaaatcag ttgcaaaagt agagggtgat cgaattattg
     4921 cgggtgctaa agcagaaata gcgcatgaaa tttttagtgc aaaagaagag ctacgccaac
     4981 aagtaagtgg tctcgtttta actggagcaa caaaaatttt gcggcgtgag attgatgcaa
     5041 aagcgcatgc tgatttatta acagcaattg aaacagagtt aaagtaatgg ctgagtcaat
     5101 aactatagct cgtccctatg cagaagctgt ttttaagctt gcaaaggaaa aaaatagctt
     5161 atcgcattgg tcggaaatgt taagtaacgc tgcgcagatt acagaagttg ctcctgttca
     5221 agaattgatt ggtaatccaa acgtttcggc atcgaagcta aaggagatta ttttcaatat
     5281 aggcggtagt aaacttgatg gcgaagtaaa gaatttgcta tcgattctga ttgacaatga
     5341 tagggttacg atgattcccc ggttagttga gatgtatgag cagttaagag caaatcatga
     5401 aggcgtctta gaggcgaata tcatttcagc cttttcaatg tcagataggc aactggagaa
     5461 attggtagct gcgcttgaga ataaattcaa tcgtaaggta aaagcgaaag taagtattga
     5521 tcaggaatta atcggtgggg taaaaatcga gattggcgac gaggttattg atagctcgat
     5581 atccggtaaa ctagaagcta tgtctattgc cctaaaaagc taggagttaa tgaaatgcag
     5641 ttgaatccat cagaaattag tgagctgatt aaaagtaaga tagaaggact atcagcgtcg
     5701 tcagaaatac gcacacaggg aaccattgta tcgctaacag atggaattgt tcgtatccat
     5761 ggactttcag atgttatgca aggtgaaatg ctggagtttc cagggaatac ctatggctta
     5821 gcgctgaact tggaaagaga ttcggttggt gcggttattt taggttccta tgagcattta
     5881 acagaaggag atacggttaa atgtaccggc cgtattttag aagttcctgt tggtgaagga
     5941 ttgcttgggc gcgttgttaa tgcattgggt cagccgatcg atgggaaggg accaatttct
     6001 gcgcaaggga aagaacccat tgagaagatt gcaccgggcg ttatctggag aaaatcagtt
     6061 gatcagccag tgcaaacggg attaaaagcg attgatgcca tggttccggt tggaagaggg
     6121 cagcgtgaat tgatcattgg tgaccgccaa actggaaaaa ctgctgttgc aattgatgcg
     6181 atcattaacc aaaaaggtga aaacatgatc tgcatctatg tagcaattgg ccaaaaagct
     6241 tcatcaattg cgaacgtagt cagaaaattg gaagaacatg gcgcaatggc gtatacgata
     6301 gttgtcgttg cttctgcgtc agaatcagca gcgatgcaat ttattgcacc ttattctggc
     6361 tgtacgatgg gggaatattt tcgtgataga ggccaagatg cattgattgt ttatgatgac
     6421 ttgaccaaac aagcttgggc ttatagacag atatcattat tgctacgccg tcctccaggt
     6481 cgtgaagctt atccaggtga cgttttctat ttgcattctc gattgttgga acgcgcggca
     6541 agagttagcg cagaatacgt agagaaatat acgaatggag ctgtaaaagg gaaaacaggc
     6601 tcacttacag cgcttccaat tattgaaacg caagcgggtg acgtaacggc attcgttcca
     6661 accaatgtga tctcgattac cgatggtcag attttcttgg aaacagactt attcaatgcg
     6721 gggattcgtc cagcaatcaa tgcaggtatc tctgtttctc gggtaggtgg tgctgcgcaa
     6781 acaaaagtta tcaaaaagct tggcggtggt attcgtttag cgcttgctca atatcgtgaa
     6841 ctagcagcgt ttgcgcaatt tgcttccgat cttgatgaag caacacgtaa gcaacttgag
     6901 cgcggcaaaa tggcgactga gcttatgaaa caacctcaat ttgcaacaca aagcgtttca
     6961 gagatggcat tgacgctatt tgctttgaac aaaggatatc tcgatgatgt tgaagtcaaa
     7021 cgtgcattag cgtttgaatc agcattacga agctatattc gtaatcaaca tgctgcattg
     7081 atcgataaga ttgagagtac caaaaatctt gatgaagata ctgagaaagc actgatacaa
     7141 gcgatcgaag aatttaagaa aaacgggact tattaatggc tggtagtaga gaaatacgca
     7201 ataagattaa aagcgtaaaa aatacgcaga agattacacg cgccatggaa atggtggccg
     7261 catcaaaaat gcgtaaagct caggaacgga tgcgtaaggc acgtccttat ggtgaaaaaa
     7321 tccgtaatgt ggctgcccat atgagtcgtg ctagtactga atataagcat ccttttttaa
     7381 ttgaacgcga taccgtgaaa cgtgttggtg ttatcgtcgt tacttcggat aaaggtttgt
     7441 gtggtggact aaacaccaat attctgcgta gagccttaaa tgagatgaaa cgatggcaag
     7501 cagagggtga agaaatcgaa gtatgctgca ttggcaataa aggcttgggt ttcatgaatc
     7561 gcattggtgc aaaagttatc tctcacgttg tcagcctagg tgatactgca aatattgaaa
     7621 agttgattgg tgcgattaag attgttctag atgggtatac caatgatcgg ttcgatcgcg
     7681 tctatgtttt ttataatcgg tttgtcaaca cgatgaagca agagccgaca atggaattat
     7741 tgttgccact gtcagatgat cggtttggta acaataaagg tgatcaggaa acgagcccca
     7801 agaaactttg ggattatatc tatgaacccg aggctaagtc tgttatcgat gatgtaatga
     7861 ttcgttacgt tgaagcactg atctatcagg cagtaacaga aaatatggca tcagaacaat
     7921 cagcgcgtat ggtggcgatg aaagccgcat cagataatgc tggcaattta atcaatgaat
     7981 tgacattgat ttacaacaaa tcgcgccaag cggctattac caaagaacta tcagaaatcg
     8041 tgggcggtgc ggccgcagtc taattttttt gtatatctat ttttaaggaa caataatgag
     8101 cgaaggaaga attgttcaat gtatcggtgc ggtaattgac gtggaatttc agaaagacgg
     8161 aattcccaat gtttatgatg ccctgactat ggaaggatca gagttgacat tggaggtcca
     8221 gcaacaattg ggtgatggtg tcgtaagaac cattgccttg ggatcttcgg atggattgcg
     8281 tcgtggaatg atggtcgtta ataccaaaaa acaaatttcg gtaccggtcg gaacaaaaac
     8341 actcggtcgc atcatggatg tattaggtcg gcctatcgat gagatgggac cagtcggcgc
     8401 tgaaaaaaca atgccgatac atcgagtcgc acctgctttt gatgaacttt ctgcttcaac
     8461 agaactcctt gaaacgggta tcaaggtgat tgatttgatc tgtccgtttg caaaaggcgg
     8521 taaggttgga ttatttggtg gtgcaggtgt tggcaaaact gtaaatatga tggagcttat
     8581 tcgtaatatt gcgattgagc acagtggtta ttccgtattt gcgggagtgg gcgaaagaac
     8641 ccgtgaaggg aatgacttct atcatgaaat gaaggattcc aatgtattag ataaagttgc
     8701 gttggtgtat ggtcagatga atgagccacc tggcaaccgt ctacgcgttg cattgacggg
     8761 attgacgatg gcggaatcgt tccgggatga aggacgtgat gtactcttct tcgttgataa
     8821 tatttatcgt tatacattag caggaacaga agtttcagca ttgttaggtc gtatgccttc
     8881 tgcagttgga taccaaccga ccttagctga agaaatgggg cgcttacaag aacgcattac
     8941 gtcatctaaa acgggctcaa ttacatcgat tcaagcggtg tatgttcctg cggatgactt
     9001 gactgatcct tcaccagcga ctacctttgg tcacttggat gcaaccgtgg ttctttcgcg
     9061 cgacattgct tcattaggta tatatccagc ggttgatccg cttgattcaa cctctcgaca
     9121 attagatccg ctcgtggttg gggaagatca ttatcaaacc gcgcgtgaag ttcaacaaac
     9181 gttacaacgc tataaagaat tacgcgacat cattgcgatc ctcggtatgg atgaattgtc
     9241 acctgaagat aaattatcag tcagtcgcgc gagaaaaatt caacgcttct tatcacaacc
     9301 cttcttcgta gctgaagtat ttaccggttc acctggaaaa tatgtgccat tgaaagaaac
     9361 tatcaaaggt ttcaaaggta tcgtgaatgg tgactacgat gatattcctg agcaagcgtt
     9421 ctacatggtc ggtggtatcg aagaagttat cgaaaaagcg aaaaaactcc aataacaact
     9481 ttctgataga gaaaatcaat gggcactgtt tttcatttgg atattgtgag tgcggaggat
     9541 tcgatttact ccggacctgc tgaatttctc gttgcacctg caatgttggg tgaagtaggt
     9601 atttttccgc aacatacacc tttactgaca cgcatcaagt caggcatggt tcgcattaaa
     9661 gcgccactca aagatgaaga atctgtctat gtatcagggg gcatgttgga agtacaaccc
     9721 gatgtggtaa ctatcctagc tgatacggcg attcgtagtc atgacttgga tgaagctaaa
     9781 gcgctcgaag cgaagaaaca tgctgaagag atcatgaagg ataaacaaag cggtattgaa
     9841 tatgcacgtg ctcaagctga attgattgaa gcttcggcac aattagcagc aatccaaaaa
     9901 ctcaggaaac gtggcggcgg ccactaatca tatttccatt cctttcgcct tgaaaggaat
     9961 ggagtcgtca ttccaatcgt aaccgtacgt tcttttaagt ttcaagaaat ttagcttata
    10021 gtggtttaca ccctatctag gctgagtttt ttcatcacgc ttagtcattc ttgtttattt
    10081 cttaacaaaa cgttacagat aattttcttt gtgctagttt ttttcatctg aaagggtgta
    10141 tattttttac tggcattctt gaaaggacgt aataatcatg gcgaaacgta ctaaaaaaat
    10201 ctacgtactg gatacctccg tattactgtt cgatcataat gcgatcaaga attttgagga
    10261 aaacgatgtt gccattccaa tcacctgctt ggaagaactc gatcgtttta aagtcggaaa
    10321 tgacattaaa aattttgaag cgcgagaagt aattcgcttc ttagataaat tatctgaaga
    10381 ttatggctta gaaaaatgga ttcccctggg tgaagagaaa gggcgattga gaatcgtcat
    10441 gtccaatagt ctaaatggta tcgatgccga agaaatctat ggtaaaggta agaatgacca
    10501 taaaatcatc aatgcagctc taacgcttgc caatagccat aaatcagcgc gagtcgtttt
    10561 agtcaccaaa gatatcaatc ttcgactcaa agccaaagcg atcggtttac cgtcagaaga
    10621 ttacgagacg ggtaaggtta aaaatgtcaa atcgatctct gcgggattcc ctattcttga
    10681 gcaaatccct tcgagcacta tcaatgaaat tgccaggcgt ggcagtatcg aagaagcggg
    10741 gctactagga acacataaac tcgcgaatgg ctattatatt cttcgtaatg ccaatgattc
    10801 cctactggca cgctataatc aaacccaaaa taccatcgag cgcgtcgaaa aagcttatgt
    10861 ctattcaatc aaacccagga atgccgagca agcgtttgca gtcaatgcgt tgctaaatga
    10921 tgatattaaa ttagtcgcgc tacaaggcgt cgcaggtacc ggaaaaaccc tgcttgcgct
    10981 cgcttctgcg ttggaactca aaaataaata cgatcaaatc atcttagcgc gaccaatcat
    11041 accgctgagt aatcgcgaca ttggttattt accagggact gccgaggata agatctcacc
    11101 tttcatgcaa ccgctctggg ataacctcaa attcatcaaa agccaatttc gccctaccga
    11161 acgcaaatcc aaaatactcg atgagatgga agaaagtggc aaactggcca ttacggccct
    11221 cgcgtttatt cgtggacgga gtttatctaa cgtgatgttc attattgatg aagcgcaaaa
    11281 cctcacgccg catgaaatca aaacgattat cactcgcgct ggagaaagta ctaaaatcgt
    11341 cttcacgggt gatattcacc aaatcgatac gccgtacttg gatgagcaat ccaatggctt
    11401 gacgtatata atcgatcgat tgaaaggcca tcctttgttt gcgcatatac gcctagaaaa
    11461 aggggaacgt tctgagttgg ccaatcttgc caacgatctg ctgtagagaa ttgaatctac
    11521 actgtattgc tactaagtca atgtttgcag actttattgc ttgagatcgg tttttcgggt
    11581 tcgtgaaagt gggctacgca cactatgatc aaccaaatcc gtcagaatca tgatgatgcc
    11641 gtagatgcca gcaagtgcca tggcctattt atcagtatga acccctttga aaatagggtt
    11701 cattgcaatg agaacaaaat acggtagcaa tagccattgg cgataccatg ccgcacatac
    11761 accatcacgc gcgcagcatc agcttgatcg actcttgaat cgattcgata cggtcgtgca
    11821 tagcagcacg tccacaatca accaattttt caaactcatt gaaattgtat ccagattcca
    11881 ccggcgtctg cgggttgatc acgatttctg cacgcaatgc ttcgctagaa gaacgccagg
    11941 agccaagcaa accccaggaa gcgctgagta catctttgag ccccaacggt ttctgggttt
    12001 tttgcagtaa tccatcgaat cccgtgatga catcattgcc actggcaaaa ggtttagtgt
    12061 tgacattaat ggcaatgcgt aatccacaac ggcggttact cagaacatcc acaggcacca
    12121 gcgaaaccac gctgccatcg accatcaaac gcgattgatt agccacgggc gggaaaaaac
    12181 ctggaattgc agaggtggcc aatactgcag gcgcgaccaa acctcggtca aagataatac
    12241 gctcatgtgt taccaaatcg gcggcgacta ccgctgcagg gtgcttaaga tcagcaaacg
    12301 tgcagtcacc aaaggtggct ttcgccgctg ctgcaatttt gttaccgatg aatagtgatc
    12361 cacggggtaa caacgatgga tccttgatct tgcggttaga gccgagttgt tcgcgcactt
    12421 gctgaatcgc ctggtcgata ctgcccaagt gtgcaaccgt caaagccaca ataccgccca
    12481 tactgctgcc acagagcaca tcgtaattaa tgccacattg agccatggca tcgagcacgc
    12541 ccaaatgcgc aaaaccgagc gcagcacctg aactcatcgc gataccgact tctttgaacg
    12601 tcaaatagcg tgcaatctta tccagggttg aattgaacgg ctcttgccag gaattcggtg
    12661 aggtgctgct tttttcgagc gctgctggca ttaattgata agcccaaggc tccaagtcgc
    12721 aataagtttg atcccgttct cctaaataaa cccgcgcaaa atcagcgact cccacttgat
    12781 ccgtgctatt gaattgttct atgtaattcg tcgtggtatc gatgatatgt agcactacat
    12841 ctttaggctc aaaataacgg ctgaggtggg gtaactccgc tgggcgaact ctgagcacga
    12901 gtaattgacc attgggggca gactcacgcc aagcattgaa cttggagtgc aattcaggat
    12961 cagccgcgtc gctaattggg ttttgcaagc ctg
//
LOCUS       DAOQKQ010000066        12945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_2256_length_12945_cov_5.844375, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000066 DAOQKQ010000000
VERSION     DAOQKQ010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 12945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12945
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2256_length_12945_cov_5.844375"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="PKZ49_05875"
     CDS             <1..465
                     /locus_tag="PKZ49_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36124.1"
                     /translation="LALAQASNWLAGLYGIFIGVIWPVYAPFAIYREETDSQTKRVMA
                     SMLLAGVGLAAYTIIGMIQEPIVAGIINHSIHYAHEVEGQQFVLAMYLFATCTPFILS
                     SRKLLNLTGTIITLGFFVAFFFYQQTFASVWCFFAAIASGLIYFFIRNQTTK"
     gene            complement(525..1019)
                     /locus_tag="PKZ49_05880"
     CDS             complement(525..1019)
                     /locus_tag="PKZ49_05880"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HND36125.1"
                     /translation="MPIKYSAVLIVFCCLVVGCSSVSQKERSPKQVRTPDSSIQTDSR
                     GVKGKLYAKYRQWEGVPYQWGGLSRSGIDCSGFVHVVYKRELGKIIPRTTELQARAGR
                     HVDINSLKAGDLVFFRIGNNTRHVGIYLENRRFMHASRSRGVTISQLDHRYWKSRYWK
                     SVRV"
     gene            complement(1102..2142)
                     /locus_tag="PKZ49_05885"
     CDS             complement(1102..2142)
                     /locus_tag="PKZ49_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HND36126.1"
                     /translation="MGYNAIPESKRLRAFRNGESRNGDVMRAVFLDFGSVTRGDLDCT
                     KLENAISSWQYYDETSPQQIVERIKTAEVVVSSKTFLDRAVIDSASHLKLICIAATGY
                     NNVDLSAAAERNIGVCNVRNYATDSVAQHVAMLMLNLVRRFVEYQSYIKHGKWQACAH
                     FCSLDYGIEDLSGKILGIIGYGELGRAVGKIGQAFGMQLRIAEHKGQPPRSGRLSFEE
                     VLSESDFISLHCPLTAQTHHLITRTELALMKSTAYLINTARGKLINEADLLESLIDGR
                     IAGAALDVLSEEPPTKGNILLTYPRPNLIITPHIAWTSQAARQCLLDKIADNITHFFE
                     GKPYNQVLEQSE"
     gene            2229..3479
                     /gene="glyA"
                     /locus_tag="PKZ49_05890"
     CDS             2229..3479
                     /gene="glyA"
                     /locus_tag="PKZ49_05890"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HND36127.1"
                     /translation="MFSKHNTIEQIDPDLWLAIKGEMQRQEDHIELIASENYASPAVL
                     EAQGTVLTNKYAEGYPGKRYYGGCKHVDIVEQLAIDRLKSLFNAEYVNVQPHSGSQAN
                     AAVYLSALKPGDTLLGMSLAHGGHLTHGASVNMSGKIFNSITYGLDPDTEEIDYKQVE
                     QLAKEHRPRMIVAGASSYARIIDWKQFRRIADEVGAYLFVDMAHYAGLIAAGFYPNPV
                     GIADFVTSTTHKTLRGPRGGVIMAKPEFEKALNSAVFPQTQGGPLMHVIAAKAVAFKE
                     AASQAFKDYQEQVLENARVMAKTLQNRGLRIVSGRTECHMFLVDLRAKNLTGKDAEAA
                     LEAAHITVNKNAIPNDPQKPFVTSGVRIGTPAITTRGFKEIETEKLANLVADVLEAPT
                     DTAVLNRVAKETQDLCAKLPVYER"
     gene            3530..3991
                     /gene="nrdR"
                     /locus_tag="PKZ49_05895"
     CDS             3530..3991
                     /gene="nrdR"
                     /locus_tag="PKZ49_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HND36128.1"
                     /translation="MRCPFCNAEDTSVINSRVSEEGDRVRRRRQCIVCGKRFTTYETI
                     ELRLPQIVKQNGNRTEFSRNKLLTGFLRALHKRPVPTEAVDAAIDRIIQHLLNSGARE
                     VSSRSIGELVMQELYNMDKVAYIRFASVYKSFQDVDDFQDVIKEVREIPKD"
     gene            4091..5194
                     /gene="ribD"
                     /locus_tag="PKZ49_05900"
     CDS             4091..5194
                     /gene="ribD"
                     /locus_tag="PKZ49_05900"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HND36129.1"
                     /translation="MPGEPSLFSAADYTFMAEAIRLAEKGLYTTSPNPRVGCVIVKNH
                     QIIGRGWHEKAGQPHAEINALKQAASEVKGSTVYVTLEPCCHYGRTPPCTDTLINAGI
                     AKLVIAAQDPNPRVAGKGIKQLKAAGIEVQYGLLEAQANQLNIGFVSRMTRQRPWITV
                     KIASSLDGKIALANGKSQWITGEAARTDVHRLRARSCAILTGINTVKQDDPELSVRYI
                     QTTRQPFRVILDNRLEIATNAKVLQKPGSLIFTTLQDNQRIDSLSEKGARVVVLSSST
                     GKLDLVEVMSTLAELEINELFVEAGRTLNGALIESRLVDEIIIYCAPIVLGNHAQSML
                     NLPELASLSQKWQLEIKEIGKVGSDIKITAKLI"
     gene            complement(5233..5733)
                     /locus_tag="PKZ49_05905"
     CDS             complement(5233..5733)
                     /locus_tag="PKZ49_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36130.1"
                     /translation="MIINQRLIVGTLFLVISASLNSLATAATENHDPLFVQTDGRMAL
                     QLSAGWNTFYSPFMYPQALLYPYPLFINPCYPYVHCGAFNQYRLLQERRKRSERSPSQ
                     HAKQRTFAFDRLALEYQRRGKLYRTNENEIVPAHKGDSKIKPEFRDSGSFLPTFLERH
                     NHSVKR"
     gene            complement(5806..8568)
                     /locus_tag="PKZ49_05910"
     CDS             complement(5806..8568)
                     /locus_tag="PKZ49_05910"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HND36131.1"
                     /translation="MELAKSFDPSEIEARWYPEWESAGYFQPLNEGGVTPYCIMLPPP
                     NVTGTLHMGHAFQHTLMDALTRYHRMLGQKVLWQPGTDHAGIATQIVVERQLDQQNLD
                     RRSLGREKFLERVWKWKEESGSTITRQMRRMGTSCDWSRERFTMDETLSKAVTEVFVQ
                     LYREGLIYRGKRLVNWDPVLQTAVSDLEVISAEEEGSLWLIRYPFEQQDGNAALTDGL
                     IVATTRPETMLGDMAVAVNPDDDRYSHLIGKHLRLPLSERTIPIIGDSYVDPTFGTGC
                     VKITPAHDFNDYQMGLRHKLVPLNIFTLDGKINEFAPSEYQGLDRFDARKRILADLKA
                     HGLLVETRPHKLMIPRGDRTQSVIEPMLTDQWYVAMEDMAKRGLDVVAQGEVKFVPEN
                     WTHTYQQWLENIQDWCISRQLWWGHRIPAWYDEVGNVTVAHDLEEAQRLSGKDQLIQD
                     EDVLDTWFSSALWPFSTLGWPKETTELKTFLPTSVLVTGFDIIFFWVARMVMMSLHFT
                     GKVPFKEVYVTGLIRDAEGQKMSKSKGNVLDPLDLIDGIALSDLIKKRTTGLMNPKQA
                     ESIEKNTRKQFPQGIPAFGTDALRFTFASLASHGRDIKFDLQRCEGYRNFCNKLWNAT
                     RFVLMNCEGKAIGFDNTPHALNYSLADKWILSRLQQTLEAVEQGFNSYRFDMVAREIY
                     EFVWDEYCDWYVELAKTQLSSENEAIQRTTRYTLIKVLETALRLSHPIIPFITEELWQ
                     KIGPLAGIKGGSIMVQSYPQSDSSAIDTEASDQIVLLKEMINACRTLRGEMKLSPAAR
                     VPLVIEGNAAVLNNFSSYISALAKLSEVTIISDGLPNDDAPVAVVRDFRLMLKIEIDV
                     VAERERLSKEISRLEGEINKAQAKLDNADFIARAPEKVVAQEKERVAEFSAKKQKLTE
                     QLARLT"
     gene            complement(8634..9035)
                     /locus_tag="PKZ49_05915"
     CDS             complement(8634..9035)
                     /locus_tag="PKZ49_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36132.1"
                     /translation="MTDHLDVPPEPEDNTLLNKLDAILKKYRKEYSAVIRPADLKATP
                     AIPEPPTPIVTPAPTQNQSDVLPSFTVPEIPILTERIVVAPEISFNESGISLLLCYAF
                     DSALKDAKLDLTSTQRFELLQALAQRLPKNL"
     gene            complement(9062..9490)
                     /locus_tag="PKZ49_05920"
     CDS             complement(9062..9490)
                     /locus_tag="PKZ49_05920"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HND36133.1"
                     /translation="MVQVDFYTGAADKLSTACRLCIKAVQQMQRMLILSDDAPQLTQL
                     DKLLWTFSSVSFIPHCFVDNQLSAITPIVLGSPAHVSPEALNYGILLNFHDEILPYYQ
                     RFDRVIEIVGHTSADKEKARKRYRIYQAQGCQVRHHNLDG"
     gene            complement(9584..11089)
                     /locus_tag="PKZ49_05925"
     CDS             complement(9584..11089)
                     /locus_tag="PKZ49_05925"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HND36134.1"
                     /translation="MDFAIKVGSADKQRSACIVVGVFESRKLTNAAKHIDVATDGYVS
                     NVLRTGDMNGKLGTTLLLHNIPTSLSKRILLVGLGQEKECNGKAFRTAILSAMSALLR
                     TAVSEVGLFLLDVSLKDRDLSWKISQTAMLASECVYRFDALKSKSEGEPPSLNKVTFY
                     LGDRAYLKIAEIALAQGKAIAQGMKVAKDLGNLAPNICTPSYLAKCAVDMGKSHKLKV
                     SVLGQKEMEKLKMGALLAVAKGSHQPAKLIALEYRGLAKTEKPIVLVGKGITFDTGGI
                     SLKPAAEMDEMKYDMSGAASVLGTLAAVAEMQLPLNVVGIIPTTENMPGGNATKPGDV
                     VTSMSGQTIEILNTDAEGRLILCDALTYAERYNPEAVIDIATLTGACVIALGHVASGL
                     MSNNDQLADELLQAAELSVDRAWRLPLWDDYQDLLRSNFADMANIGGRSAGTISAACF
                     LARFAEKYQWAHLDIAGTAWKSGKEKGSTGRPVPLLTQFLISRAMRKVAHN"
     gene            11325..12803
                     /locus_tag="PKZ49_05930"
     CDS             11325..12803
                     /locus_tag="PKZ49_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HND36135.1"
                     /translation="MKENLIQQLRFCSTLPSLPAVALKIIDLANSADTDMIQISHYIA
                     LDPALAAKILKTANSPLYKSRRTSSNIRQAVCVLGTHAVISIALSFSLTKTLMQHTES
                     SIGKESSDLFWRRSIIAALACKALGNKINLKMADDLFLAGLLQDIGILAFFSIIPEAY
                     AKIYSSAASHDALLQAEYEAFETGHDELGYTLLKRWNLPSYVAIACMTSHNQSSSQKG
                     EPTIADCVAASGYIADYFLNPSDTEKIAQTMTKLYARLNLDGQALLKVIAKIKDELRT
                     VEELFEFSICSESELNALMSEAQELLSIQSLIKIRDLEDKTQRDGLTGAHNRAFFDSV
                     FEREFIFSTQHSTPLTIAMIDVDHFKQVNDSHGHVVGDALLIALVRAIFGQIRQDDLL
                     CRYGGEEFVLILPGTPIASARKLLIRLKNSIEAISYKLDNDKHIGITVSMGIATNLDK
                     GSIFTSHDSMLEAADLALYAAKHAGRNKIVEWDHSLSLQNHS"
BASE COUNT         3699 a         3009 c         2632 g         3605 t
ORIGIN      
        1 cttgcgcttg cacaagcttc taattggtta gctggtttat atgggatttt tattggggtt
       61 atttggccgg tttacgcgcc gtttgcaatt tatcgagaag aaacggatag tcaaaccaag
      121 cgagtcatgg cttcaatgct attagcaggg gtaggccttg ctgcatatac gattattgga
      181 atgattcagg aaccgattgt agcgggcatt atcaaccata gcattcatta tgctcatgaa
      241 gtggaaggcc agcaatttgt tcttgccatg tatttatttg ctacttgcac accctttatt
      301 ttatcgagca gaaaattgtt gaatcttacg ggaacgatca ttacgctcgg tttttttgtt
      361 gcattctttt tctaccagca aacctttgct tcggtttggt gtttctttgc agcgattgca
      421 agtggcctaa tctatttctt tattagaaat cagactacta aataaggtat tctcctatca
      481 gctttcatta caagctttat tcatttacta cattcatcag ccagctaaac cctcactgat
      541 ttccaatagc gtgatttcca atagcgatga tctaattgtg aaatggtaac accccggctc
      601 cgagaagcat gcatgaacct ccgattctcg agataaattc caacatgtcg tgtgttatta
      661 ccgattctaa aaaaaaccaa atcccctgct ttcaatgaat tgatatctac atgccgaccc
      721 gccctcgctt ggagctcagt ggttcgagga ataattttac ctaattctcg cttgtaaacc
      781 acgtgcacaa aaccggaaca atcaatcccg ctacgactta acccgcccca ttgataagga
      841 acgccttccc attgccggta tttggcatat agcttgccct ttacaccacg cgaatcagtt
      901 tgaatgctcg aatcaggtgt tcgtacttgc tttgggcttc gttctttttg actgactgaa
      961 ctacaaccaa ccacgagaca acaaaagaca atgagaacgg cactgtattt gattggcatg
     1021 ttataagttt atagagatcg ataatgaaga gtctatcaaa aaaaaataat ttggaaaata
     1081 acgaataagg gccttcccag cttattctga ttgttcaagc acttgattat aaggttttcc
     1141 ttcgaagaaa tgggtaatgt tatcggcaat tttatcgagc aagcattgcc tggcagcctg
     1201 gctagtccaa gcaatatgag gcgtaataat caaattaggg cgagggtaag ttaggagtat
     1261 attacctttt gtcggtggtt cctcagacaa aacatcaagt gctgctccgg caatacgccc
     1321 atcaattaaa ctttcgagta gatcagcttc attgatcaat ttgccgcgtg ccgtattgat
     1381 taaataagcg gtagatttca tcagtgcaag ctcagtacgc gtaatcagat gatgtgtttg
     1441 cgcagtcagc gggcaatgca aactaataaa atccgattca ctaagtacct cctcaaagct
     1501 aagtcttcca gaacgagggg gttgcccttt atgttcagct atccgcaatt gcataccgaa
     1561 tgcttgtccg atttttccta ctgctcgacc tagctcacca tatccaatta tgccgagaat
     1621 tttcccagat aaatcttcta ttccgtaatc cagagaacaa aaatgggcgc aagcctgcca
     1681 tttaccatgt ttgatatagc tttggtattc aacgaaacgg cggaccaaat tgagcatcaa
     1741 catggccaca tgctgagcaa ccgaatcggt tgcataattc ctcacattac aaacgccgat
     1801 attgcgctca gccgcagcgc taagatcgac attgttatag ccagttgctg caatacaaat
     1861 caatttcaag tggctagctg aatcaatcac cgctcgatcg aggaaagttt tactgctcac
     1921 gacgacttcc gctgttttaa ttcgttctac gatctgttgt ggactggttt catcgtagta
     1981 ctgccaagac gaaattgcat tttctaactt cgtacaatca agatcgcctc gggtaacaga
     2041 cccaaaatct aagaacactg cacgcataac atctccattc ctactttctc cgttgcgaaa
     2101 cgccctcaac cgctttgact cgggtattgc attgtaaccc atgcgttaag tccctacaca
     2161 agatagatca aggtggtaga atggaaagct acctgttttg ataaatttca taaagagaag
     2221 atacggtcat gttttctaaa cacaatacaa tagagcaaat tgatccagat ctatggttag
     2281 ccattaaagg tgaaatgcaa cgacaagaag atcatattga actgattgct tcagaaaatt
     2341 acgcaagccc tgcagttctt gaagcacaag gtacagtatt gaccaacaaa tatgcggaag
     2401 gttacccggg caagcgctat tacgggggat gtaaacatgt cgacatcgtt gaacaactcg
     2461 ccatcgatcg cctgaaatcg ttgttcaatg ccgaatatgt taacgtacag ccccattctg
     2521 gctcacaagc caatgcagcg gtttatcttt ctgcccttaa gcctggagat actttactgg
     2581 gtatgtcgct tgctcatggc gggcacttaa cgcatggtgc atcggtcaat atgagtggaa
     2641 aaattttcaa ttcgattact tatggtttag accctgacac cgaagaaatc gattacaaac
     2701 aagtagaaca actggccaag gaacatcgtc ctcgcatgat tgtcgcgggc gcctcatctt
     2761 atgcccgaat tatcgactgg aaacaattcc gtcgaattgc tgacgaagtt ggcgcctatc
     2821 tttttgtaga catggcccat tacgctggac tcattgctgc aggattttac cctaacccag
     2881 tgggtattgc ggatttcgta acgagtacca cgcataaaac acttcgaggt ccaaggggtg
     2941 gcgttatcat ggctaaacct gaatttgaga aagcactcaa ttcagcagtg tttccgcaaa
     3001 cccaaggagg tcctttgatg catgtcatcg cggctaaagc agtggcattc aaagaagcgg
     3061 caagccaagc tttcaaagat taccaagaac aagtgttgga aaatgcccgt gtgatggcaa
     3121 aaacattgca aaaccgtgga ttacggatcg tttcaggccg taccgaatgc cacatgtttt
     3181 tggtcgattt acgtgccaag aatttaaccg gaaaggatgc agaagcagca ttagaagctg
     3241 ctcatatcac tgtcaataaa aatgcgatcc ccaatgatcc tcaaaaacct ttcgtaacca
     3301 gtggggtacg aattggaaca cctgcaatca ccacacgcgg ctttaaggaa atcgaaaccg
     3361 agaagttagc caatctcgtg gcagatgtat tggaagcccc taccgataca gccgtattga
     3421 atcgggtcgc aaaagaaact caggatttat gtgcaaagct tcccgtgtat gaacgataaa
     3481 agaccgtgct ttgggtaatg atcagttcga tttctgatct attaaggtaa tgcgatgccc
     3541 tttttgtaat gctgaagata caagcgtcat taattcacgc gtcagtgaag aaggtgatcg
     3601 ggtaagacgt cggcgccaat gtatcgtatg tggtaaacgt tttactacct atgaaacgat
     3661 cgaattgcgt ttaccacaaa tcgtcaaaca aaacggaaat cggactgaat ttagtcgtaa
     3721 taaattgctg acgggctttt tgcgtgcttt acataaacgg cccgtcccca ctgaagcggt
     3781 tgatgcggca atcgatcgga ttattcagca tttgctaaat tctggcgcac gtgaagtttc
     3841 atcccgtagc attggagaac tggtaatgca ggaactttat aacatggata aagttgccta
     3901 tatccgtttt gcttcggtct acaagagctt tcaagatgtt gatgatttcc aggatgtcat
     3961 taaagaagtc cgggaaatcc ctaaagatta atcatcgcat tgtaaaactt gatcgcaaat
     4021 cctttttata tcgcttcttt aataaaattg atgatagtgt acagttaggt gtaacgatta
     4081 tttgcacata atgcctggcg aaccatcact cttttctgca gcagattaca ccttcatggc
     4141 tgaagccatt cggcttgcag aaaaaggctt atataccacg agccctaacc cacgagttgg
     4201 ctgcgtcatt gtaaaaaatc atcaaattat tgggcgagga tggcatgaaa aagcgggaca
     4261 accgcatgct gaaatcaatg cgctaaaaca ggcagcctct gaagttaaag gcagtacggt
     4321 ttatgttacg cttgaaccct gttgtcacta tggccgtact cccccctgta cagatacttt
     4381 gatcaatgca ggtatcgcta aattggtcat tgctgcacaa gaccctaatc cacgagttgc
     4441 cggaaaagga ataaaacaac tcaaagcagc tggtattgaa gttcaatatg gtctattgga
     4501 agcgcaagca aaccaactca atattggttt tgtctcacga atgacgcgcc aacgcccctg
     4561 gattaccgtc aaaatcgcat cgagcctaga tggaaaaatc gccttagcaa atggtaaaag
     4621 ccagtggata actggggaag cagccagaac agacgtgcat cgattaagag cccgttcctg
     4681 cgcaattctg accggaatca acaccgtaaa acaagatgat cctgagctat ctgtccgcta
     4741 tattcaaaca actcgacaac catttcgtgt catcttagat aatcgacttg aaatcgcgac
     4801 gaatgccaag gtattacaga aacccggaag tttaattttc actaccttgc aagacaacca
     4861 aagaatcgat tcgttatcgg aaaaaggcgc tcgcgtcgtt gtcttatcca gcagcacggg
     4921 gaaactcgat ctcgtggaag tgatgtcaac attagctgag cttgaaataa atgaattatt
     4981 tgtagaagca ggcagaacat tgaacggcgc gctcattgaa agccgactcg tcgatgaaat
     5041 cattatttat tgcgccccga tagtattagg caaccatgcc caaagtatgc ttaatttgcc
     5101 tgagctagca tcgctttctc aaaaatggca gctagaaata aaagaaattg gtaaggttgg
     5161 tagtgacatt aaaatcacag ccaaactcat ctgaattttc taatgctttc tttcaattgc
     5221 tatccccata cactaccttt tcactgaatg attatgtcgt tctaagaatg taggtagaaa
     5281 tgacccgcta tcgcggaatt caggctttat tttgctatcc cctttatgcg ctggaacaat
     5341 ttcattttcg ttggttcgat acaatttgcc tcgtctctga tactccaatg cgagccgatc
     5401 aaatgcaaac gttctttgtt tagcatgctg actcggagat ctttccgatc gttttctccg
     5461 ttcttgtagt aaccgatatt ggttgaatgc accgcaatgc acatacggat agcaaggatt
     5521 aatgaacagt ggataaggat agagtaacgc ctgagggtac ataaacgggc tgtagaaagt
     5581 attccatccg gcactgagtt gtagcgccat cctaccgtct gtttggacaa acaatggatc
     5641 gtgattttct gttgctgccg ttgcaagcga atttaaactc gctgaaataa cgagaaacaa
     5701 agtgccaact ataaggcgtt gattaattat catgatgaag ctgatccttc tcaaaggtct
     5761 tagctatttg atacattaac caaatttttc gttcccgcat cacgattagg ttagccgcgc
     5821 cagttgttcg gttaatttct gtttttttgc gctgaattct gcaactcgct ctttctcttg
     5881 tgcaactacc ttctctggtg cacgtgcgat aaaatctgca ttatcgagct ttgcctgtgc
     5941 tttattgatt tcaccttcca agcgagagat ttctttagac aaacgttcac gctctgcaac
     6001 aacatcgatt tcaattttga gcatcaaccg gaaatcgcgt actaccgcaa cgggtgcatc
     6061 atcattaggt aatccatcgg aaatgatggt aacttccgag agtttagcta aggctgaaat
     6121 ataggaagag aaattgttaa gtactgcagc attgccctca atcaccaatg gaaccctcgc
     6181 tgcaggggat aatttcattt caccccgcaa cgtgcgacac gcattaatca tttctttgag
     6241 tagtacaatt tgatcgctgg cttccgtatc aatcgcgctt gaatccgact ggggataaga
     6301 ttgcaccatg atacttccac cctttatacc tgctagtggg ccaatttttt gccacaattc
     6361 ttcagtaatg aaaggaataa tgggatgaga caacctaagc gcagtctcta acactttaat
     6421 caacgtatag cgcgtagtcc gttgaatcgc ttcattctca gaacttaatt gtgtttttgc
     6481 aagctcgaca taccaatcac aatattcatc ccaaacaaac tcatagattt cacgcgcaac
     6541 catatcaaag cgataactgt taaaaccttg ttctactgct tccaaggttt gctgcaagcg
     6601 actgagtatc catttatctg ccaaagagta attcaaagca tgcggcgtgt tatcgaatcc
     6661 aatcgctttc ccttcacaat tcatcaatac aaagcgcgtg gcattccata atttattaca
     6721 gaaattccga taaccctcac agcgttgcag atcgaatttg atatcacgcc catgtgaggc
     6781 aagacttgcg aacgtaaaac gcagcgcatc ggtaccaaat gctggaatac cttgaggaaa
     6841 ttgtttgcga gtatttttct cgatggattc cgcttgtttc ggattcatca atcctgtcgt
     6901 gcgctttttg attaaatccg acaaagcaat cccatcgatc agatcaagcg gatcgaggac
     6961 attccccttc gatttactca ttttctgccc ttcagcatca cggatcagcc ctgttacata
     7021 cacctctttg aagggcactt ttccagtgaa atgaagtgac atcatcacca tgcgcgctac
     7081 ccagaaaaaa atgatgtcga atccggttac caacactgaa gtcggtagaa atgtttttaa
     7141 ttccgttgtt tcctttggcc aacccaatgt agaaaaaggc cacaaggctg atgaaaacca
     7201 agtatcaagc acatcttcat cttgaattag ctgatccttc ccagataatc gctgcgcctc
     7261 ttccagatca tgcgcaaccg ttacattacc gacttcatca taccaagcgg gaatacgatg
     7321 tccccaccaa agctggcgtg aaatacacca atcttgaata ttttctagcc actgctgata
     7381 ggtatgagtc cagttctctg gaacaaattt gacttcacct tgggcaacta catcaaggcc
     7441 gcgtttcgcc atgtcctcca tcgccacata ccactgatca gtcaacatgg gctcgatcac
     7501 cgattgagtg cgatcgcctc tcggtatcat tagcttatga gggcgtgttt caaccaataa
     7561 cccatgcgct ttaaggtccg ctaagatcct ttttcgggca tcgaatcggt ccaacccctg
     7621 atactcggaa ggtgcgaact cattaatttt accgtcaagc gtaaagatat tgagcgggac
     7681 tagcttatgc cgcagtccca tctggtaatc attgaaatca tgcgcgggcg taatttttac
     7741 gcaacccgtc ccaaaagtag gatccacata actatcccca ataatcggga ttgttcgctc
     7801 agaaagtggc agacgtaagt gcttcccaat caaatggcta tagcgatcat cgtcgggatt
     7861 cactgcaacg gccatatccc ccaacatcgt ctcaggtcga gtggttgcaa caatcaatcc
     7921 gtcggtgagc gcggcgtttc catcttgttg ctcaaacggg tagcgaatca gccaaagcga
     7981 accttcttct tccgctgata tcacttccaa atcagatacc gccgtttgta aaacggggtc
     8041 ccagttaacg agtcgttttc cccgataaat caacccttcc cgatataact gcacgaaaac
     8101 ttctgtaact gcctttgaaa gcgtttcgtc catggtaaac cgttcacgtg accagtcaca
     8161 ggaagttccc atgcgcctca tttgccgggt aatcgttgaa ccggattctt ctttccattt
     8221 ccagacccgt tcaaggaatt tttcccgccc aagactgcgt cgatccaaat tctgctgatc
     8281 gagctgtcgc tcaaccacaa tttgcgttgc gattcctgcg tgatcggttc cgggttgcca
     8341 caacactttc tgacctaaca tccggtgata acgcgtcaat gcgtccatca acgtatgttg
     8401 aaatgcatgc cccatatgca acgtcccagt cacattgggt ggtggcagca taatgcagta
     8461 aggtgttacg cccccttcat tcaacggctg aaagtaccct gccgattccc attcaggata
     8521 ccagcgtgct tcaatttcac ttggatcaaa actttttgca agttccataa ataatataaa
     8581 tagagcagac tatgaattta gagattataa ctaaagaaag cgtaccgatt ctctcaaaga
     8641 tttttaggta atcgttgggc aagtgcttga agcagctcaa aacgctgtgt tgaggtcaga
     8701 tcaagttttg catccttaag cgctgaatca aacgcatagc agaggagtaa cgaaataccc
     8761 gactcattaa atgatatttc cggagcaacg acgattcgct cggttaaaat tggaatttct
     8821 ggaacagtaa aactgggtag tacatcagac tgattctgag ttggggctgg ggttacgatg
     8881 ggagttggag gttctggtat cgcaggcgtc gctttaagat ccgccggacg gatcaccgca
     8941 gaatattctt ttcgatattt cttcaatatc gcatcgagtt tattgagcag cgtattatct
     9001 tctggttcag gtggcacatc gagatgatca gtcatcaaat aatatcccta ttcattgtca
     9061 atcaaccatc taagttatgg tgtcgaacct gacagccttg tgcttgatag atgcggtatc
     9121 gtttacgcgc tttctcttta tccgctgaag tatgaccaac gatctcaata actcgatcaa
     9181 agcgttgata gtaaggcaaa atttcatcat gaaaattaag caaaatacca taatttaacg
     9241 cttcaggaga aacgtgggct ggagaaccca atacgatcgg ggtaatcgca gatagttgat
     9301 tgtccacgaa acaatgtgga atgaaactga ccgaagaaaa agtccagagc agtttatcca
     9361 actgagtgag ttgcggtgca tcatcactca gtataagcat tctctgcatt tgctgaacgg
     9421 cctttataca tagacggcag gcagttgaaa gcttatctgc cgcacctgta taaaaatcaa
     9481 cttgtaccat tcctgctaat catgctgatc taactacgat tgaattaggt gcgctagatt
     9541 tgccaagatc caattgcatt caagtcgctt cggtagccta gcctcagtta tgtgccactt
     9601 ttcgcatcgc ccgtgaaatt aagaattgcg tgagtaaagg aactggacgt ccggtagaac
     9661 ctttttcttt tccagacttc caagcagtac ctgcaatatc aagatgcgcc cattggtatt
     9721 tctcagcaaa tctcgccagg aaacaagctg cagaaatcgt tcctgccgat cttccaccaa
     9781 tattcgccat atctgcaaaa ttgcttctta ataagtcttg gtaatcatcc cataaaggta
     9841 atcgccaagc gcgatctacc gataactcag cagcttgcaa taattcatcc gccaattgat
     9901 cattgttact cattaaccca gaagcgacat gaccgagtgc aatgacacaa gcgcccgtca
     9961 atgtagcgat atcaatgact gcttcaggat tgtaacgctc tgcataggtt aatgcgtcac
    10021 atagaatcaa ccgtccttct gcatctgtat tcagtatttc gatagtttgc ccagacatac
    10081 tggtcacgac atctcccggt ttagtcgcat taccaccagg catattttcg gtggttggga
    10141 taatacccac tacattgagc ggcaactgca tttcagcgac tgcagccaaa gttcccaaca
    10201 cactggcggc accactcata tcatatttca tctcatccat ttccgctgcc ggtttgagtg
    10261 aaataccgcc ggtatcaaag gtaatccctt taccgaccaa tacgatcggt ttttctgttt
    10321 tggcgagccc tcggtactcc aatgcgatta atttagccgg ttggtgactc ccttttgcca
    10381 cagccaatag cgcacccatc ttcagcttct ccatctcttt ctgaccgagt actgagacct
    10441 tcaatttgtg agacttaccc atatcaacag cgcatttggc aagatagcta ggagtacaaa
    10501 tattgggtgc taaattaccc aaatctttag ctactttcat accctgagca attgctttcc
    10561 cctgtgccaa agctatctca gctattttga ggtatgctcg gtcaccgaga tagaacgtta
    10621 ctttattcaa cgacggaggc tcaccctctg atttggattt aagtgcatcg aatcgataaa
    10681 cacactcact cgcaagcatt gctgtttgag atatcttcca ggatagatca cgatctttca
    10741 aggaaacatc cagcaaaaac aaaccgactt ccgatacggc agtacgcagc agcgcactca
    10801 tcgctgacaa aattgccgtt ctaaacgctt taccattgca ttccttttct tggcctaaac
    10861 cgactagtaa aatacgttta ctcagactgg taggaatatt atgcaatagt agcgtagtgc
    10921 ccagttttcc attcatatct ccagtacgaa ggacattact tacataacca tccgtagcga
    10981 catcgatatg tttagctgcg ttcgtcagtt tacgcgattc aaaaacacct accacgatac
    11041 aagcactgcg ttgcttgtca gcgcttccta ccttgattgc gaaatccaca ttccactcct
    11101 tgtcagttat tcgatagcaa ttcaattgga ttatcgctgg actcccggta caaagtcaaa
    11161 agaagccaag caattcaaat cgaattttat tcataatgac aaactctagc attttttgcc
    11221 ttcttattct ttcttttcat taggttgcat cgcttagaat gcattatgac tactaaagga
    11281 aaaattaact attctcaatt tttataggcg atagagggaa ggtaatgaaa gagaatctca
    11341 tccaacaact gcgtttttgc tcgacattac cgagtctacc tgcggtcgca ctcaagatta
    11401 tcgatctagc taatagtgct gatactgaca tgatccaaat tagtcactac attgcgctag
    11461 atccggcatt agcagcaaaa atactaaaaa ctgctaattc cccattgtat aaatcgagaa
    11521 gaacatctag caacatccgt caggcggttt gtgtattagg tactcatgcc gtcatcagca
    11581 tcgcgctttc attctcgctg acgaaaacat tgatgcagca cacagaatcc agtatcggaa
    11641 aagaaagtag tgatcttttt tggcgtagat cgatcattgc agcacttgca tgtaaagcgc
    11701 taggcaacaa aattaacttg aaaatggcgg atgatttgtt tcttgctggt ttattacaag
    11761 atatcggtat cctcgccttc ttctcgatca ttcctgaagc gtatgccaaa atctattcat
    11821 ccgccgcctc ccatgatgct ttgcttcagg cagaatacga agcgtttgaa acggggcatg
    11881 acgagctagg ctatacctta ctcaaacgct ggaatttacc gagctatgtg gcaatcgctt
    11941 gcatgacgag ccataatcaa tcttcttctc aaaagggaga accaacgata gccgattgcg
    12001 tcgccgcatc ggggtatatc gcagattatt ttttaaaccc aagcgatacc gaaaaaatag
    12061 cgcaaaccat gactaagcta tatgctcgac ttaacttaga tggtcaagct ttactcaaag
    12121 ttatcgcaaa aatcaaagat gagctcagaa cggtcgagga attatttgaa tttagtatct
    12181 gcagtgaaag tgagctcaat gcgctcatgt cggaagctca agaattgtta tcgattcaat
    12241 ccttaataaa aatacgtgat ctcgaagaca aaacacaacg cgatggattg actggagcgc
    12301 ataaccgcgc tttcttcgat tcagtctttg aacgtgaatt tatcttttct acccaacact
    12361 caaccccgct aaccatcgca atgatcgatg tcgatcattt caagcaggtg aatgatagcc
    12421 atggacacgt ggtgggagat gcattgctca tcgcactggt cagagccatt tttggacaga
    12481 ttcgccaaga tgatttgtta tgtcgctatg ggggtgaaga atttgtcctc attctacccg
    12541 gaacacccat tgcctcagcg cgcaaactgc ttatccgact aaaaaatagt attgaagcca
    12601 tctcctataa gctcgacaat gataagcaca ttgggataac cgtatcgatg ggcattgcca
    12661 cgaatcttga taaagggagt atttttactt cccacgatag catgttggaa gccgctgatt
    12721 tagcgctata tgcagctaaa catgcaggtc gcaataaaat tgtcgaatgg gatcattcac
    12781 tctccctgca aaatcacagt tagtatcgct ccaaaaaatt agtatcattc aatctcgcat
    12841 ttcagttata cacacaactt gccaatttgc gtgtgtaacg cctaagctat ctctgcaaaa
    12901 aatttaatct atactcagaa attttatgaa tttaatcatt caagg
//
LOCUS       DAOQKQ010000067        12932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_2259_length_12932_cov_6.117263, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000067 DAOQKQ010000000
VERSION     DAOQKQ010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 12932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12932
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2259_length_12932_cov_6.117263"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="PKZ49_05935"
     CDS             <1..435
                     /locus_tag="PKZ49_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HND36136.1"
                     /translation="AVLAAKIVSLRQSVKVIPLGILMGIVVMVMTLVNNLWIAIGLLI
                     LIGGLAGFFVVPMNALLQHRGHILMGAGHSIAVQNFNENLSILVMLLLYALLIWLDVH
                     IYVVIILFGAFVSISMTIIRKWHLTNQSKEDSLHLIGMRKIH"
     gene            complement(432..1169)
                     /locus_tag="PKZ49_05940"
     CDS             complement(432..1169)
                     /locus_tag="PKZ49_05940"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HND36137.1"
                     /translation="MNQRRKEIMRELGLTPFWRKKQGRTPTTVDTKIHDSNESDSQHL
                     DRTKRILRLDWPQLKAEVENCHACPLSQARLHTVFGVGDEQADWLFIGEGPGAREDEI
                     GEPFVGQAGKLLDSMLAAIQLKRGNNVYITNIVKCRPPNNRNPECNEAEQCRPYLERQ
                     IALIKPKLIIALGKVAAQNLLTTNDSISNLRGRLHSYLGTPLIVTYHPAYLLRSLMDK
                     SKAWEDLCFAKATMESLAITTNPSLKI"
     gene            complement(1166..1627)
                     /gene="rimI"
                     /locus_tag="PKZ49_05945"
     CDS             complement(1166..1627)
                     /gene="rimI"
                     /locus_tag="PKZ49_05945"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HND36138.1"
                     /translation="MNLSIKPIPSIREMSLNDVEGIILIEREIFLFPWTPVNFTDSIK
                     AGYQCRVMEYGDAIVGYGILMLGLDEGHLLTIGIAAQWHHRGLGTQLLQYFIDLARSK
                     QLTSFLLDVRESNTGALYLYQRMGFKQIGVRKGYYPAMCGREDALVMRLEL"
     gene            complement(1624..2298)
                     /gene="tsaB"
                     /locus_tag="PKZ49_05950"
     CDS             complement(1624..2298)
                     /gene="tsaB"
                     /locus_tag="PKZ49_05950"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HND36139.1"
                     /translation="MNIFALDTSTEYCSLALWLDGEILSRSVLAGQKHSELVLPMLNE
                     MLSEGGITLSHLDGFAFGAGPGSFTGLRIACGVIQGLAFALDKPVVDISTLEALAQQN
                     GSAKVITALDARMGEIYFAAYTQKPNNQWIPILSPMLCRPELAPQLIGKDWVGCGSGF
                     DRYPDFLRDVYHDQLNEILTGCYPHAREIAQLSIARFEQGSVLLPEQAVPTYIRNKVA
                     LKESER"
     gene            complement(2336..2884)
                     /gene="thpR"
                     /locus_tag="PKZ49_05955"
     CDS             complement(2336..2884)
                     /gene="thpR"
                     /locus_tag="PKZ49_05955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HND36140.1"
                     /translation="MIELQSERIPIKVFFAIWPDLHVKTQLSRLTGNLEHLCGGYRTN
                     SLKLHLTLVFLGNILTKQLAYVQEIASQIKLPCFSLTIDTIEYWKHNKIVYAGITDTP
                     VELIKLVSQLHHDLQLAGFKLEQRSYIPHLTLIRNATCQQLPKLTTPIHWNIKEWMLI
                     QSTPTKTGMVYSQLSSWKLIEK"
     gene            complement(2888..3382)
                     /gene="ispF"
                     /locus_tag="PKZ49_05960"
     CDS             complement(2888..3382)
                     /gene="ispF"
                     /locus_tag="PKZ49_05960"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HND36141.1"
                     /translation="MKNLRIGQGFDVHQLIEGRKLIIGGVTIPYEKGLLGHSDADVLL
                     HALCDALIGAAALGDIGKHFSDKDNRYKDIDSRLLLREVHQLIKQKHYDVINIDATII
                     VQAPKMAPYILAMIANIAEDLKMPSSDINIKAKTAEKLGFIGRGEGIVAEVVCLIAKT
                     DELD"
     gene            complement(3379..4083)
                     /gene="ispD"
                     /locus_tag="PKZ49_05965"
     CDS             complement(3379..4083)
                     /gene="ispD"
                     /locus_tag="PKZ49_05965"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HND36142.1"
                     /translation="MSQFFALIPAAGFGSRMGHQLPKQYLPLVEKPMIYHALHTLVNC
                     SKITRVFVILAPDDDIWAHYNWTELQDKLVVEYCGGDARAESVLNGLNAMQSSGMVNR
                     DDWVLVHDAARPCLSAPQLDQLINELSYDGVGGLLAIPVADTLKRADADHRIISTESR
                     ERLWQAQTPQMFRFNLLKDALSQVDSHQITDDASAIEALGLKPKLVLSDAYNFKVTYP
                     QDLALAELILQKREAK"
     gene            complement(4206..5543)
                     /locus_tag="PKZ49_05970"
     CDS             complement(4206..5543)
                     /locus_tag="PKZ49_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HND36143.1"
                     /translation="MASLNQRVLVSASLVLILFLAGTAVTLEQAFHDSSKKALQDRML
                     GKLYLLMGEAEVNESGLFQMPAEVPGLIEFEQLNSGIYAYVSDQSDRVIWESKSSLSI
                     ELPIPKHLAEGDEVFEPIMLAHVPHFLYSYGVAWETESGGHYPFTFHVIFDSTSFEEQ
                     IDRYRSDLWGLFAVMAIVLLLTQMMVLRWGLKPMRQVSAELNAIEAGLQDKIQGDYPI
                     ELKRLTDNINSFVYRERKHQQRYRNALADLAHSLKTPLTIAKGAISTEHDSQTLIDTI
                     GEQLDRMNNIVHYQLQRASTAGVSSRMSYLELRPIIERLIHTIERAHLEKHPNVSYVI
                     SPDIRLRMDEGDLMELLGNLIDNAYKWCHRAITIKAKQESNHTIISIENDGPGIQPEE
                     VDKILERGVRADQSIPGHGIGLAIVRDIVQAYDGTLSIQSNGDTGVSFIIKLK"
     gene            complement(5543..6226)
                     /locus_tag="PKZ49_05975"
     CDS             complement(5543..6226)
                     /locus_tag="PKZ49_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HND36144.1"
                     /translation="MRILIIEDEIKLQRQIQLQLEAQGFVVDTCSNGKEGLYFASEYP
                     LDAAIIDIGLPDLSGLDVIAALRKQGSLLPILILTARNSWQEKVKGLEMGADDYLTKP
                     FQMEELLARLKALLRRATAVPDTEIRSGAILLDMEAQTVKLNSQLVDLTAFEYRLLEH
                     LMRHQGEVISKQELTDYLYPHNDDHDSNVLEVMVGRLRRKLDPDGSLNPIETLRGRGY
                     RFIAIAQAS"
     gene            complement(6269..6550)
                     /locus_tag="PKZ49_05980"
     CDS             complement(6269..6550)
                     /locus_tag="PKZ49_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HND36145.1"
                     /translation="MMKFQSKVLVVMLWVALVSGESFAQMKLASELSDEQIEISQQQA
                     VTIAQRYTHGRLLSVQRMDDHYRIKILNHKGAVHIVTINAKNGEVDLSQ"
     gene            complement(6612..6965)
                     /locus_tag="PKZ49_05985"
     CDS             complement(6612..6965)
                     /locus_tag="PKZ49_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36146.1"
                     /translation="MNTQFNRAKITRFVTIALLSVALIVPIAAEAGRGHHRHHHHHKH
                     HHHDHHLHGHSGYGGYGYYGKPRYNNYRYVERHYYSQPYTYYAPAYSGYYYEGVPGNF
                     YLGINAGNGNFMLGY"
     gene            complement(7101..7427)
                     /locus_tag="PKZ49_05990"
     CDS             complement(7101..7427)
                     /locus_tag="PKZ49_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36147.1"
                     /translation="MKKHFVITTLVFFLIHLFFANVYAESPVRKSVETRKVDLDHDRE
                     NNMRNTEMKQHQKSKSLQKAKNEEKQNSSHSDESKHRQELEQSYKKQWEEVEKGSGDP
                     RLVIGR"
     gene            complement(7551..8906)
                     /locus_tag="PKZ49_05995"
     CDS             complement(7551..8906)
                     /locus_tag="PKZ49_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HND36148.1"
                     /translation="MAFRYHFFRYSFVSFVFANICWVNTVMGAGFAIMEQSVLELGRA
                     FSGAPTNTGDGSMVYFNPGAMTQVRSKLLTTAGYLIVPSAKFNDEGSQLNPLVGGFPL
                     RGNEGGNAANLIPIPNVYYIQPLTSKVSFGLGVNSPFGMENQYHSNWKGRYQAITSEL
                     ITININPSLAVKLTEKVSLGAGLNIQYLQAKLSNAVDFGAICLQAIGLGACGSRGLLP
                     QSADGMVTLKGDSVGFGFNLGLYFEPSQITRLGISYRSRVEHEVKGTANYAVPDQALI
                     LTRGGQFIDTHTQTRVIMPDQVMLGFSQYLGARWNLTGDALWTNWSLNRELRTDFASP
                     QGDSIQQLNWNDTWRLALGLSYTPEVQKWTIRTGFAFDQTPVPNADFRPARIPDNDRY
                     WLTAGFSYRVLSNLTLHGAYAHLFIPDPAINSKSATGDRLSGQYTAQVNIVGLQLDWR
                     F"
     gene            9189..11561
                     /locus_tag="PKZ49_06000"
     CDS             9189..11561
                     /locus_tag="PKZ49_06000"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HND36149.1"
                     /translation="MIDQNPPTPFDNRAQEIRAGQIKLLFQQTPSAFLATLLNSVILA
                     SILWGNVPKNQLISWIITVYILTLTRYLFVRYYHQIKPSSAYAIVWGRFFLLGVFLSG
                     TLWGIAGKIFFIQDSLLHQLFLIYLLGGMVAGAMSTLSSYRGAFLTFSIPVTLPVCYE
                     AFSLGSEFHIAIAATFLLFLIMLWAISQRLHLMITDSLRLRFDNADLLEHLLQAKNEQ
                     QTINEALQTQILEKERAQKTLQNINAQLEQHVMERTHDLVLTNDSLHREKELFRVTLA
                     SIGDAVITTDAKGNITYLNPIAESLTGWNNTESMGIPLHKVFQTVDQETQEYLVNTLF
                     NRLATATTINKSQECLLVRKDQQESVVDYSVAPIHNDEGNVYGIVLTFRDVTEQRNLA
                     RKLAYQASHDPLTGLLNREAFENRLNKILGAAREDNVHALLYLDLDQFKVVNDTCGHS
                     AGDELLRHVTTLLQSQLRARDTLARLGGDEFDIILEHCSKKDAIKIAQTLREAIQDYR
                     FHWQDKSFTIGASIGLYPINRSGESLSTVLSAADSACYAAKETGRNRVHVYQSDDHLL
                     QKRTGEMQWLPRIQKAIIENRLCLFFQPILATHSDPTIEKHGEILLRLRDEEGNLIMP
                     GAFLPPAERYDQMLLIDRWVVEKAMVFIKAELQHSTKVIYAINLSAQALCSEDFLDFI
                     VTQIKTHINHPASLCFEITENVALADPKHVKQFIATLKELGCLFSLDDFGSGFSSFSY
                     LKEIPIDFLKIDGRLIKDMTSDPIDLTNGAIDPKYRTRHGSQNYCGMGRR"
     gene            11524..11652
                     /locus_tag="PKZ49_06005"
     CDS             11524..11652
                     /locus_tag="PKZ49_06005"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HND36150.1"
                     /translation="MGLKTIAEWVEDEATLQLLRDMNVDYAQGFWLARPQQLDEIN"
     gene            complement(11769..12524)
                     /gene="moeB"
                     /locus_tag="PKZ49_06010"
     CDS             complement(11769..12524)
                     /gene="moeB"
                     /locus_tag="PKZ49_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HND36151.1"
                     /translation="MNDDQLLRYSRHILLPQIDINGQEKLSRSCVLIVGLGGLGCPAA
                     LYLAASGINKLIICDPDLVDLTNLQRQILHTTQSVGEAKTSSAERALYQINPTIEIIS
                     LKKRITEDVLQEYISQVDAIIDASDNFITRNLISQMCYHHKKPLISGAVTGFNGQITV
                     FDHRKQENPCYQCLFPELSTDSDVNCAVMGVFAPLAGVIGSIQAAETIKVLLDIGTTL
                     SGRLLLLDGLTMQLRTIKINKDPDCHVCGAVAV"
     gene            complement(12584..>12932)
                     /locus_tag="PKZ49_06015"
     CDS             complement(12584..>12932)
                     /locus_tag="PKZ49_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HND36152.1"
                     /translation="PNGQSIQAKGITPDIVDESEESSRQLRESDLSRHLRSNKSESGD
                     DNTNGGEPKKQNEAAGNKTKSKKATDSAKKKTAPAEFGSSDDALLAKAITYLKNSNGN
                     IKRKNTKTAKSAG"
BASE COUNT         3760 a         2891 c         2446 g         3835 t
ORIGIN      
        1 gctgttttag ctgccaaaat cgtttccctg cgtcaatcgg taaaagtcat tccactgggg
       61 atactgatgg gcattgtcgt gatggtcatg acactcgtta ataatctttg gatcgcaatc
      121 ggattactca ttctcatcgg tggtctagcg ggtttttttg tggtgccgat gaacgcgcta
      181 ctgcagcatc ggggtcacat tctcatgggt gctgggcact ccatcgccgt ccaaaatttc
      241 aatgaaaatt tgagcattct cgtcatgttg cttttatatg cattgttaat ttggctagat
      301 gtgcatatct atgttgtgat catcttattt ggtgcgtttg tctcaatttc gatgacaatt
      361 attcgcaaat ggcacctaac caaccaaagt aaagaagact cgctacatct cattggtatg
      421 cgtaaaattc attaaatttt gagcgatggg tttgtagtta ttgcgagtga ttccatcgtg
      481 gctttggcaa agcataaatc ttcccaagct ttagatttat ccattaagga tcttaggaga
      541 tacgcagggt ggtatgtcac gattaagggt gtgcccaaat aagagtgcaa tcgcccccgt
      601 aaatttgaaa tactatcatt ggtggtgagt aaattctgcg ccgcaacttt gcccagcgcg
      661 atgattaact tgggtttgat taaggcaatc tgccgttcta gataggggcg gcattgttca
      721 gcttcattac attcaggatt tcgattattc ggtggtcgac acttaacgat attggtaatg
      781 taaacattgt taccgcgttt taattgaatc gcagcgagca tgctgtccaa taatttgcct
      841 gcttgcccca caaaaggctc tccgatttca tcttctcgag cacctggccc ttccccaata
      901 aatagccaat ctgcttgctc gtcccctaca ccgaaaaccg tatgtaaccg agcctgactc
      961 aatggacatg cgtggcaatt ttcaacctcg gcttttagct gcggccaatc caaacgaaga
     1021 atgcgcttgg tgcgatccaa atgctgacta tctgactcat tgctatcgtg tattttagta
     1081 tctaccgtcg tgggtgttct accctgcttc tttctccaaa aaggcgttaa gcccagttca
     1141 cgcattattt ctttgcggcg ctgattcaca attctaatct catcacaagc gcatcctcac
     1201 ggccacacat tgctggataa taacctttgc gcaccccaat ttgtttaaag cccatacgtt
     1261 gatagagata gagggcaccc gtattcgatt cacgaacatc gagtaaaaaa gaagttaatt
     1321 gcttcgaccg tgctaaatcg ataaagtatt gtaacagctg tgttccaaga cctcgatgat
     1381 gccattgggc cgcaatacca atggttagga ggtgcccctc atctaggccc aacattaaaa
     1441 taccataccc aactatcgca tcaccgtatt ccataacgcg gcattgatac cctgctttga
     1501 tcgaatcagt aaagttaacc ggtgtccagg gaaataaaaa aatttctcgc tcgattaaaa
     1561 taataccttc aacatcgttt aaagacattt cacgaataga gggtatcggt tttattgata
     1621 aattcatcgc tcgctttcct tgagcgcaac tttattccga atataagtag gcacagcttg
     1681 ctctggcaaa agtactgaac cttgctcaaa gcgagcaata gaaagttgtg caatttctct
     1741 tgcatggggg taacagccag tcagaatctc attgagttga tcatgataga catctcttaa
     1801 gaaatcgggg tatcgatcaa agccactccc gcatccaacc caatcttttc cgattaattg
     1861 aggagcaagt tcaggtcgac atagcatcgg tgataggatc gggatccact gattatttgg
     1921 cttttgggta taagccgcaa agtaaatttc acccatacgg gcatccaaag cggtgattac
     1981 ctttgctgag ccattttgtt gcgccagtgc ctcaagcgta ctgatgtcaa caacaggttt
     2041 atctaatgcg aaagcaagtc cctgaatcac accacaagct atgcgtaaac cggtaaaaga
     2101 accgggacct gcaccaaacg cgaaaccatc taagtgcgat aaagttattc caccctcact
     2161 cagcatttca tttagcatgg ggagaactaa ctctgaatgt ttttgcccag cgagcacact
     2221 gcgactcaat atttcaccat cgagccataa cgcaagagaa caatattcag tagaagtatc
     2281 cagtgcaaaa atgttcattc aacttagcaa tcgcttgggt aaatacaaaa agggattatt
     2341 tctctattaa cttccaactg cttaattgag aatagaccat acccgttttc gttggcgttg
     2401 attgtattaa catccactcc ttgatattcc agtgaatcgg tgtcgtcaat ttaggaagtt
     2461 gctgacaagt tgcatttcta attaacgtta aatgcggaat atatgagcgt tgttccaatt
     2521 tgaatccagc caactgtaaa tcatgatgca gttgactaac taattttatt aactcaacag
     2581 gtgtatcggt gataccggca taaacaattt tattatgttt ccaatattcg atcgtatcaa
     2641 tggtcaaact gaaacaaggt agcttaattt ggcttgcgat ctcctgaaca taagcgagtt
     2701 gcttcgtcaa aatattgcca agaaaaacca gcgttaagtg caatttaagc gaattcgttc
     2761 gataaccccc acaaagatgc tcaagatttc ccgttagtcg gctcaattgc gtttttacgt
     2821 gtaaatctgg ccaaattgca aaaaaaacct taatcggtat tctttccgat tgcaactcaa
     2881 tcacgtgcta atcaagttca tcagttttag caatcaagca aacgacttct gcaacaatgc
     2941 cctctccacg accaataaaa ccaagcttct cggctgtttt ggctttgata ttaatatcac
     3001 tggaaggcat ttttagatct tcagcaatgt tagcaatcat cgcaagaata taaggggcca
     3061 ttttaggagc ctggacgata atggttgcat cgatattgat gacatcatag tgtttctgtt
     3121 tgattaattg atgtacttca cggagtaata agcggctatc gatatctttg tagcgattat
     3181 ctttatcaga aaaatgcttg ccaatatctc ccaaagcagc agccccaatc aatgcatcgc
     3241 ataaggcatg tagtagcacg tctgcatccg aatgccctaa caaacctttt tcataaggaa
     3301 tcgtaacgcc accaatgatg agttttctac cctctatcaa ctgatgcaca tcaaaacctt
     3361 gtccaattcg tagattcttc atttcgcttc ccttttttgc agaatcagtt ctgctaaggc
     3421 taaatcttgg ggataagtaa ctttaaaatt atacgcatca ctcaatacga gtttaggctt
     3481 caacccaagc gcttcgattg cactcgcatc atcggtaatt tgatggctat ccacttgact
     3541 taaggcgtct ttcagtagat taaaacgaaa catttgggga gtttgtgctt gccataatcg
     3601 ttctcttgac tcggtcgata taatgcgatg atcagcatct gcacgtttta gggtatctgc
     3661 aactggaatt gctaataaac caccaacccc gtcataagat aactcattga tcaattgatc
     3721 gagctgtggc gcacttaaac aaggccgcgc tgcatcatga accagtaccc aatcatctct
     3781 atttaccatc ccacttgatt gcatcgcgtt caatccattg agcacacttt cagcacgcgc
     3841 atcccccccg caatactcaa ctactagctt gtcttggagc tctgtccaat tgtaatgtgc
     3901 ccaaatatcg tcatctgggg caagaattac aaaaacacgc gtaattttgg aacaattgac
     3961 tagcgtatgt aacgcatgat aaatcattgg tttttcaacc aaaggaagat attgtttagg
     4021 caactggtgc cccattcttg aaccaaaacc tgctgccgga atcaacgcaa aaaattgaga
     4081 catatggttt atttaaatcg ctgagtagtt ttcactaacc aataattatc gcacacccta
     4141 gatagctatc cacgttcgtt agcagaaaat tattcaacgc ctcaactttc tgagattacg
     4201 attaatcact tgagcttaat aatgaaagat acgcctgtat ctccatttga ttgaatagat
     4261 aacgttccat cataggcttg cacgatgtca cgtacaatgg caaggccaat tccatgccct
     4321 gggattgatt gatctgctct aaccccccgc tctaaaattt tatcaacttc ttcaggttgg
     4381 ataccaggcc catcgttttc aatgctgatg atcgtatgat tgctttcctg tttcgctttg
     4441 atcgtaatag cccggtgaca ccatttataa gcattgtcaa tgagattacc caataactcc
     4501 atcaagtctc cttcatccat tctgagacga atatcagggc taatcacata actcacgttg
     4561 ggatgttttt ctaaatgggc tcgctcaata gtatgaatga gcctctcgat gataggcctt
     4621 aattcaagat aactcatcct tgaagaaacc ccagcagtgg aagctcgctg taattgataa
     4681 tgaacaatat tgttcattcg atcgagttgc tcaccgatgg tatctatcaa agtttgtgaa
     4741 tcatgctctg tactaattgc tccttttgca atggtaagtg gcgttttaag actgtgcgcc
     4801 aaatctgcca acgcattacg atagcgctgc tgatgtttac gttctcgata aacaaaagaa
     4861 ttaatattat ccgtcaaacg tttaagttca atcggataat ccccttgaat tttatcttgc
     4921 aggcctgctt caattgcatt caattcagcc gatacttgcc gcatcggttt aagtccccaa
     4981 cgtaatacca tcatctgcgt aagcagcagt acaatagcca tcacagcaaa taacccccat
     5041 agatcactcc gatatcgatc aatttgctct tcaaaagaag tgctatcaaa aatgacatgg
     5101 aaggtaaatg gataatgtcc accactttca gtttcccacg ctacaccgta gctataaaga
     5161 aaatggggga catgcgccag cataattggt tcgaacactt catcaccttc tgcaaggtgt
     5221 ttaggtatag gcagttcaat actaagcgat gatttagatt cccaaatgac tcgatctgat
     5281 tgatctgaaa cataagcata aatccctgaa ttcaattgct caaattcaat taatccaggc
     5341 acttctgcag gcatttgaaa caatccagat tcgttaacct cggcttcccc catcaataaa
     5401 tagagtttac ctaacattct atcctgcaaa gctttcttac tactatcatg aaacgcttgt
     5461 tccagggtaa ctgctgttcc tgctaaaaat aaaataagaa ctagggatgc actaacaaga
     5521 acgcgttgat tcagtgaggc cattaggaag cttgggcaat ggcaataaaa cgataacctc
     5581 tccctcgtaa ggtttcaatc ggattgagtg aaccatcggg atcaagcttg cgccttagcc
     5641 gtccaaccat gacttccaag acattgctat catgatcatc attatgcgga taaagataat
     5701 cagtcaattc ttgttttgaa atcacttcgc cctgatgacg catcagatgt tctaaaagcc
     5761 gatactcaaa ggcagtcaaa tcaaccaatt gactatttag ttttacagtt tgtgcttcca
     5821 tatcgagcaa gatagcacca cttctgattt ctgtgtctgg aactgccgtt gcacgcctta
     5881 acaaagcctt gagtctagct aatagctctt ccatctgaaa aggcttggtt aaataatcat
     5941 ctgcccccat ttccagaccc ttaacttttt cttgccaact atttcgtgca gttaagatta
     6001 atatcggtaa taagctaccc tgtttccgca aagcagcaat gacatctaat ccagatagat
     6061 caggcaagcc aatatcaata atggcagcat ccagaggata ttcacttgca aaatataaac
     6121 cttctttgcc attgctgcac gtatcaacca caaatccctg cgcttcaagc tggagttgta
     6181 tttgtctctg taatttaatt tcatcttcaa taatcaaaat acgcatgata ggttaggcaa
     6241 tagtaacaat taaaagctga atgctatttt attgggataa atcaacttca ccatttttag
     6301 cattaatggt cacaatatga acagcccctt tatgattgag gatcttaatc cggtagtgat
     6361 catccattcg ttgcacagaa agtaaacggc catgggtata acgttgagca atagtgacgg
     6421 cttgctgttg cgatatttct atttgttcat cgctcaactc actcgccagc ttcatctggg
     6481 caaaactttc tcctgatacc aaagccaccc ataacatcac aaccaatacc ttgctttgaa
     6541 atttcatcac acaccgtgta aatcaagata aatgttcact gatggaatca tcaaacgtta
     6601 agatacttat ctcaatagcc tagcataaaa tttccatttc ctgcattgat ccctagataa
     6661 aaattacccg gaacgccttc atagtaataa ccgctatagg caggagcata ataggtataa
     6721 ggttggctat agtaatggcg ctcaacatag cgatagttat tgtaacgagg tttaccataa
     6781 tagccataac cgccatagcc actatgacca tgtaaatgat gatcatgatg gtgatgttta
     6841 tgatggtggt ggtgacgatg atgacctctt cctgcttcag cagcgatggg gacaattagc
     6901 gctacgctca gtaacgcgat cgtaacgaaa cgagttattt tagctcgatt aaattgcgtg
     6961 ttcatgtgat ctctcctcta aagtttgttc attgagcaga ataatttctg caatgaacat
     7021 cactctacag ggagatacat gaacaaaaac tgaatcgttt gataaatgat ggaatgatta
     7081 caacttcgcg taaaacagat ttaccgtcca ataacaagac gagggtcgcc gcttcctttc
     7141 tcaacttctt cccattgctt cttataagat tgctctaact cctgtcgatg tttgctttca
     7201 tcagaatgcg atgaattctg tttttcttca ttttttgctt tttgaaggct tttacttttt
     7261 tgatgctgtt tcatttcagt atttctcata ttattttcac gatcgtgatc aagatcaact
     7321 tttctggttt caacgctttt tcttactggt gattcagcat aaacatttgc gaaaaacaaa
     7381 tggatgagaa aaaaaactaa ggttgtaatt acaaaatgct ttttcatgat tgaattcctt
     7441 ctattgattg aaatgaggtt agaaataaac actttgcagt gtaaaacaca cttcactcga
     7501 tcaaattgcg aataaaaagg aattttgcca aacgtgcatc aagataaccg ttagaaacgc
     7561 caatctaact gtagcccgac gatattaact tgtgccgtat attgcccact tagacgatct
     7621 ccagttgcac ttttactatt tattgccgga tctgggatga acagatgcgc ataagcgcca
     7681 tgtagggtta gattggacaa tacacgataa ctaaaacctg ccgttaacca ataccgatca
     7741 ttatcaggaa tgcgtgcagg tctaaaatca gcattgggta cgggtgtttg gtcaaaagca
     7801 aaacctgttc taatcgtcca cttctgtacc tcaggtgtat aactcaaccc taaagccaat
     7861 cgccaagtgt cattccagtt gagttgctgt atagagtccc cttggggtga agcaaaatca
     7921 gtccgtaatt cacgattgag actccaattg gtccacagtg catcacccgt caaattccac
     7981 cgagcaccga gatattgtga aaaacccaac atcacctggt ccggcataat aacgcgcgtc
     8041 tgagtatgcg tatcgataaa ctgccctccc cgcgttagaa ttaaggcttg atcaggtacc
     8101 gcataatttg cagtgccttt aacttcgtgt tcgactctcg atcgataact gatcccaagg
     8161 cgggtgattt gactcggttc aaagtagagc ccgagattaa aaccaaaccc gacactatct
     8221 ccctttaaag ttaccattcc atcagctgat tggggtaata atcctcggct gccgcaagcg
     8281 ccaagaccaa ttgcttgcaa gcaaattgcc ccgaaatcga ccgcattgga taattttgct
     8341 tgtaaatatt gaatatttaa ccctgcgccc agtgaaactt tttcagttaa ctttaccgca
     8401 agtgaaggat taatgttaat ggtaatcaac tcagaagtaa tggcttgata acgccctttc
     8461 caattggaat ggtattgatt ttccatacca aaaggagaat taacacctaa cccaaatgat
     8521 acttttgatg ttaatggctg tatgtagtaa acattaggaa tcggaattag atttgctgca
     8581 ttgcctccct cattaccgcg taatggaaaa ccacctacta agggatttaa ttgggaaccc
     8641 tcatcgttaa attttgctga tggaacaatt aaataaccgg cagtcgttaa cagtttgctc
     8701 cgaacctgag tcatcgcacc aggattgaaa taaaccatac ttccatcccc agtgttggta
     8761 ggtgcccccg aaaaagcacg tcctagctct agtacacttt gctccataat ggcaaaccct
     8821 gcacccatca cggtattaac ccagcaaatg ttggcaaaca cgaaacttac aaaactgtat
     8881 cgaaagaaat gatatctaaa agccatctat ttactcattt gttgtcaaaa tgacatctgt
     8941 acgctaccga gttaattaaa tagaaccctt aacatattat tttctactta tctaataatt
     9001 agatgacatg atactgcggc acatttgatg ttacgtcaat gagttatgca ataatgggta
     9061 tatcttcaaa aaaattcatg attgaatatt gttttatgct ttggttttag cagtttttat
     9121 caccataata atcattatga cgtcactgat acgctatgct aactaaacca taagaattgc
     9181 tacttacttt gatcgatcag aaccctccca ctccttttga taatcgggcc caagaaattc
     9241 gagccggaca aatcaagctg ctttttcagc aaacacccag tgcattctta gcaactttac
     9301 ttaattcagt tattctggca tcgattctct ggggtaatgt tccaaaaaat caattaatta
     9361 gctggattat tacagtttat attttgacgt taacccgata cctatttgtc cggtattacc
     9421 atcaaataaa accatccagt gcatatgcta tcgtttgggg acgattcttc ttactcggtg
     9481 tttttttatc cggtacattg tggggcattg ctggaaaaat cttctttata caagacagcc
     9541 tacttcacca gctttttcta atctatctac tcggtggaat ggtagcgggt gcaatgtcta
     9601 ccttatcttc ctatcgcggg gcttttctca ctttttcgat tcccgttaca cttccggtct
     9661 gttatgaagc gttctcactc ggaagtgaat tccacattgc aatcgctgca acttttttat
     9721 tgttcttaat catgctttgg gcgatttctc agcgattgca cttgatgatt accgactcac
     9781 ttcgattacg ttttgataat gcagatttat tagagcatct tctgcaagca aaaaatgagc
     9841 agcaaacgat taatgaagca ctacaaaccc aaattctaga aaaagagcga gcacaaaaaa
     9901 cactgcaaaa tatcaatgca caacttgaac agcacgtgat ggaacgcacg catgatctcg
     9961 tgctgacaaa cgattcattg caccgagaaa aagaactctt ccgagtcaca ctcgccagta
    10021 ttggcgatgc cgtcattacc acggacgcaa agggaaatat cacttactta aatccgattg
    10081 cagaatcact aaccggctgg aacaatacag aatcgatggg cattccactg cataaagttt
    10141 ttcagactgt cgatcaggaa acccaagaat acttagtcaa tacgcttttc aatcgtttag
    10201 caactgcaac aacgataaat aaaagccaag aatgtttatt ggtccgcaaa gatcagcaag
    10261 aatcagtcgt cgattattca gttgcaccaa tccacaatga tgagggaaac gtttatggta
    10321 tcgtcctcac tttccgcgat gttaccgagc agcgtaactt ggcaagaaaa ctcgcgtacc
    10381 aagcctcgca tgatccttta actggattac ttaatcgaga agcctttgag aacaggctta
    10441 ataaaattct gggggccgcc cgtgaagaca atgtccatgc gctcctttat ctcgatttag
    10501 atcaattcaa agttgtcaat gacacctgtg gacacagtgc aggtgatgaa ttattgcgtc
    10561 acgtcacaac acttttacaa agccaactaa gagcgcgtga cacccttgcc cgactcgggg
    10621 gcgatgaatt cgacatcatt ctggagcact gctcaaaaaa agacgcgatt aaaattgcac
    10681 aaacgcttcg ggaggcgatt caagattacc gtttccactg gcaagataaa agctttacga
    10741 ttggtgccag cattggttta tatccgatca accgcagtgg agaaagcctt tccactgtgt
    10801 taagtgcagc cgatagcgct tgttatgcag ccaaagaaac tggacgtaac cgggttcatg
    10861 tgtatcaaag tgatgatcat ttgctgcaaa aacgtactgg agaaatgcaa tggttaccgc
    10921 gcatccaaaa ggctatcatc gagaaccgcc tatgcctttt ttttcaaccg attctagcca
    10981 cgcatagcga cccaacaatt gagaagcatg gcgaaatctt attacgatta agagatgaag
    11041 aaggtaattt gattatgcct ggtgcgttcc ttcctccggc tgagcgatat gatcaaatgc
    11101 tactcattga tcgctgggtc gttgaaaaag ccatggtttt cattaaagcc gaattgcaac
    11161 acagcaccaa agtcatatat gcgatcaatt tatctgctca agccctgtgt agcgaagatt
    11221 ttcttgactt cattgtgact caaatcaaaa ctcatattaa tcaccctgca agcctctgtt
    11281 ttgaaatcac cgagaatgta gcactcgctg atcccaaaca cgtcaagcaa ttcattgcta
    11341 ctttaaaaga attgggttgc ttattctctt tagatgattt tggcagtggt ttttcatcat
    11401 ttagctactt aaaagaaatt cctatcgatt ttctgaaaat cgacggtcgc ctcatcaaag
    11461 atatgaccag tgaccctatc gatctaacca atggtgcaat cgatccaaaa tatcggacac
    11521 gtcatgggtc tcaaaactat tgcggaatgg gtcgaagatg aagctacctt gcaattgctg
    11581 cgtgatatga atgtcgatta tgcacaagga ttctggctgg ctcgaccaca gcagttggat
    11641 gaaatcaatt gacttcagca aattctactg atgcatagcc attcttacaa aattcgactg
    11701 gtaatagatg ccctctacta agtctttttt gttttctacg cgttagcgat aatcatttta
    11761 tgacgtagct aaacggctac tgctccacac acgtgacagt ctggatcttt gttgattttt
    11821 atagttctta actgcattgt taaaccatca agcagcaata agcggcctga gagcgttgta
    11881 ccgatatcaa gaagtacctt gatggtttca gcagcttgaa tgctccctat tacaccggct
    11941 aacggtgcaa aaacacccat tacggcacaa ttgacatcac tgtcggtgct caattccgga
    12001 aataagcatt gataacaagg gttttcctgc ttgcggtgat caaaaaccgt tatttgtcca
    12061 ttaaatccag taacagcacc tgatattagc ggttttttgt ggtgataaca catttggctg
    12121 ataagattgc gtgtgatgaa gttatcactt gcatcgatga tggcatcaac ttgagaaata
    12181 tattcttgca acacatcctc agtgattcgt tttttcagcg aaatgatttc aatcgtggga
    12241 ttaatttgat aaagcgctcg ctcagcagag cttgttttag cttctccaac tgattgcgtc
    12301 gtatgtagta tctgcctttg taaattggtt agatcgacca gatcggggtc acagataata
    12361 agcttattga ttccacttgc tgcaagataa agtgctgctg ggcaccccaa tccgcccagc
    12421 cctacaatga gtacgcacga tctacttaac ttttcttgcc cgttaatatc aatctgaggg
    12481 agtaagatat gccgactgta acgaagcagt tggtcgtcat tcaatcacgt acctttccaa
    12541 ttagatttct cagttagcgc taaacaaata acttgtatcg caattaaccc gctgatttag
    12601 ctgttttagt attttttcgt ttgatattcc cattactatt ttttaggtaa gtaatcgctt
    12661 tagctaacaa tgcgtcatca cttgatccaa actcagctgg tgccgttttt ttcttagcgc
    12721 tatcggtagc tttttttgat ttagttttgt taccagccgc ctcattttgt tttttaggtt
    12781 ctccaccatt cgtattatca tcaccacttt cactcttgtt actgcgtaaa tggcgactta
    12841 gatctgattc acgaagttgg cgtgagcttt cttctgattc atcaacaata tccggtgtaa
    12901 tacccttagc ctgaatcgat tgcccattcg gc
//
LOCUS       DAOQKQ010000068        12827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_2288_length_12827_cov_5.734732, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000068 DAOQKQ010000000
VERSION     DAOQKQ010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 12827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12827
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2288_length_12827_cov_5.734732"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..237)
                     /locus_tag="PKZ49_06020"
     CDS             complement(37..237)
                     /locus_tag="PKZ49_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36153.1"
                     /translation="MIDSALMEIEYWGSHGNSVSRIQSIKEGMNHAVNYISLRYDLFN
                     PSIRKSRLILNYLAIYGRDELK"
     gene            236..1357
                     /gene="wecB"
                     /locus_tag="PKZ49_06025"
     CDS             236..1357
                     /gene="wecB"
                     /locus_tag="PKZ49_06025"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HND36154.1"
                     /translation="MQKKIYLVAGARPNFMKIAPIIRALQEKETLAYRIVHTGQHYDR
                     EMNEVFFEELGIPEPDIFMAAGGGSHSQQTAKIMLAFEEYCLNDRPDAVIVVGDVNST
                     LACSIVAKKLHIPVAHVEAGLRSGDLTMPEEINRLVTDSISDWFFVTEPSGVEHLKRE
                     GKADDAIFFVGHVMVDNLLYQASKLTHINLEESEISKFKLDRTLSKQRYGVITLHRPS
                     NVDDREVFSRIGKALKEISTHLPLIFPTHPRTRENIKKFGIDLGSNITLVGPQAYMPF
                     LDLWKNAAVVLTDSGGLQEETTALGIPCITIRENTERPITVDEGTNTLVGTNPERIIQ
                     EVDNLLQGKGKQGHRPQLWDGHAAERIVEILEKKLLQRV"
     gene            1411..2871
                     /locus_tag="PKZ49_06030"
     CDS             1411..2871
                     /locus_tag="PKZ49_06030"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HND36155.1"
                     /translation="MHSLIPVILSGGSGTRLWPLSREKYPKQLLPLINEDSLLQATIK
                     RLDGLTGITVENPLIVCNEEYRFVIAEQLRLIGKACSILLEPFGRNTAPALTLAALVA
                     AQNNADPILLVMPSDHVIVNASAFQACISKGVSSALQNSIVTFGIAPDAPETGYGYIQ
                     TGELLGDVGITQIARFVEKPNQQTAQAYIDDGSYLWNSGIFMVRASVWLAAINQCRPD
                     IFSTCQTAWNKGSHEGNFIRVNETAFTACPNDSIDYAVMEKLSSNHPSLPSGVVIEFS
                     GGWSDVGAWDSLWQVLPKDEAGNVSRGDVILHECKNTLAISENRLVACVGVENLTVVE
                     TPDAILVVHKNRTQDVKKIVDSLKLTKRVEVILHRKIFRPWGWYDAVDAGERFQVKRI
                     VVKPGAALSLQMHHHRAEHWIVVRGTAKVTRDDKSYLVSENESTYIPLGTRHRLENPG
                     RLPLEMIEVQSGSYLGEDDIVRFEDIYGRNEAYKPN"
     gene            3011..3307
                     /locus_tag="PKZ49_06035"
     CDS             3011..3307
                     /locus_tag="PKZ49_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HND36156.1"
                     /translation="MPIYEYICRSCGFEKEHLQKMSDAPIAQCPECGSHEYVKLVSAA
                     GFQLKGSGWYVTDFRNKPTSKSETQTKPNTSAGDNTTTTSEKTATPTSSTGTAE"
     gene            3471..5252
                     /gene="aspS"
                     /locus_tag="PKZ49_06040"
     CDS             3471..5252
                     /gene="aspS"
                     /locus_tag="PKZ49_06040"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HND36157.1"
                     /translation="MRTNYCGAIDKTYLDQEITLYGWVHRRRDHGGVIFIDLRDREGI
                     VQIVCDPDHKENFQIAEKIRTEYVLKIVGLVRHRPAGTTNSGIFSGEIEVLARAIEIL
                     NASLTPPFQMDEENLSEAIRLEYRYLDLRRPSMQQNIRLRHRVSMAIRTFLDQAGFID
                     VETPILTKSTPEGARDYLVPSRVHDGHFFALPQSPQLFKQLLMVSGFDRYYQITRCFR
                     DEDLRADRQPEFTQVDIETSFLSENEIMDLMEKMIRHLFLNAIDIDLPNPFPRMTFAE
                     AMHKYGSDKPDLRVPLVLTELTDLMQEVTFQVFRDAAQKSGGRVAALRIPQGGELSRK
                     EIDDYTQFVAIYGAKGLAYIKVNDRNKGLEGLQSPILKFLPETVIQAILERTQAQNGD
                     LIFFGADKAKIVNDALGALRVKIGHERGLAEKNWKPLWVIDFPMFEWDEEEKRWKALH
                     HPFTSPIEGHENLLTNDPEKALSRAYDMVLNGVEIGGGSVRIHRQEVQAKVFHALGIT
                     EHEAQEKFGFLLNALQYGAPPHGGIAFGLDRIVAMMTGTESIRDVIAFPKTQRAQCML
                     TQAPNSVTEKQLRELHIRLRNPSASIS"
     gene            5265..5702
                     /gene="nudB"
                     /locus_tag="PKZ49_06045"
     CDS             5265..5702
                     /gene="nudB"
                     /locus_tag="PKZ49_06045"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HND36158.1"
                     /translation="MHAYKLPVSVLVVVYTIDRDVLLLERADHPGYWQSITGSQDPDE
                     SLWQTAVREIKEETGLVANRFELNDWHIQNQYEIFQEWRWRYAPGVTHNTEHVFGLKL
                     PEIMPISIASREHLSLIWLPWHEAAEKVFSWSNAEAIRKLDTT"
     gene            5856..7229
                     /gene="glmU"
                     /locus_tag="PKZ49_06050"
     CDS             5856..7229
                     /gene="glmU"
                     /locus_tag="PKZ49_06050"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HND36159.1"
                     /translation="MSDLDIVILAAGVGKRMRSSLPKVLHKLAGKPLLKHAIDTAQTL
                     SPRKIYIVYGHGGEQVLTEINDDQLTWVKQEPQLGTGHALLQVLPFLAKSGKTLVLLG
                     DVPLIQENSLTQLVASASANNFALLTVTLANPFGYGRIIREDGTNQVSAIVEEKDANE
                     IQRSINEINTGIMVLPNAYLHDYLPKLSNSNAQGEYYLTDIIKLAASSGIKIETAQPH
                     YEWEVSGINSKVQLAQLERAAQNFQAQQLLDAGVTLMDPSRIDIRGNLICGNNVEIDV
                     NCIFEGTVTLGDNIKINANCIIRDTIISSGTTVLPFSLIDNAEIGKNCHIGPYARIRP
                     GTRLDDNVHIGNFVEIKNSYIAMDSKANHLSYIGDATIGKKVNIGAGTITCNYDGAYK
                     HQTIIEDNVFVGSDTQLIAPVKVAKGSTIGAGSTITRDTPEEQLTLSRAKQISVAGWK
                     RPSKTKV"
     gene            7277..9124
                     /gene="glmS"
                     /locus_tag="PKZ49_06055"
     CDS             7277..9124
                     /gene="glmS"
                     /locus_tag="PKZ49_06055"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HND36160.1"
                     /translation="MCGIVGAVAKDNVVPILLEGLSRLEYRGYDSAGIVVANAELQRL
                     RTTERVSELGRLVATKKTQGLTGIAHTRWATHGAPSERNAHPHFSGQDKKIAVVHNGI
                     IENHEQIRKRLEKEGFEFLSDTDTEVIAHLISFNLETTPNLLEAVCKTINELQGAYAI
                     AVVEESQPDHIVVARNGAPLLLGVGNNGNYVASDASALIQVTKHMIYLEEGDVAELQS
                     NGYRVLNRIGNAAYKEVTRLVRESDLTRDSVELGPYAHYMQKEIFEQPIAVANTLEMV
                     LNAHSISPQLFGTEAEAIFKKTNGILILACGTSYHAGLVAKYWLETIAGLPCNVEIAS
                     EYRYRDPIANPDCLVIGISQSGETADTLAAINYAKTLGHKHSLSICNVPESALIRQTN
                     LRFLTRAGPEIGVASTKAFTTQLAALMLLTVTLAKLRNRFSGELEQEVITSLRHLPIA
                     IQSALSVEPQVKAWAANFSQKRHALFLGRGVHYPIALEGALKLKEISYIHAEAYAAGE
                     LKHGPLALVDKEMPVIAIAPNDALLEKLKSNLQEVRARGGELYVFADSDSHIQESEGI
                     HIIRLAEHAGMLSPLLHTIPLQLLAYHVALQKGTDVDKPRNLAKAVTVE"
     gene            complement(9187..9618)
                     /locus_tag="PKZ49_06060"
     CDS             complement(9187..9618)
                     /locus_tag="PKZ49_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HND36161.1"
                     /translation="MFNPTRNQARQLFFDTWYKYGRHELLSGMETIALEVILQHTEYH
                     PILEDYDQFIDKDYLPEFGETNPFLHMSMHMAIKEQLSINQPVGIQERYRNLVVKLGD
                     EHAAIHNVMDCLGEMLWHAQRDQTSPNAQIYLDCLDKAIRS"
     gene            complement(9621..10280)
                     /gene="nth"
                     /locus_tag="PKZ49_06065"
     CDS             complement(9621..10280)
                     /gene="nth"
                     /locus_tag="PKZ49_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HND36162.1"
                     /translation="MNAAKRQEMFSRLKSLNPHPTTELVYSTPFELLVAVILSAQATD
                     KSVNLATQKLFLVANTPAKILALGEVRLSEMIQRIGLYRTKAKNILATCKLLNDLYEG
                     QVPRNREDLEQLPGVGRKTANVVLNTAFGEPTIAVDTHIFRVANRTGLAPGKNVLEVE
                     NKLLKRVPAPFQHDAHHWLILHGRYTCKARKPACHECCISDLCEFDEKNLYLTKRDLE
                     S"
     gene            complement(10277..10957)
                     /locus_tag="PKZ49_06070"
     CDS             complement(10277..10957)
                     /locus_tag="PKZ49_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HND36163.1"
                     /translation="MSSNENDSLIRQIDAILPQTQCGQCGYSGCLPYATAMAQGVADI
                     NQCPPGDETVIHQLAVLLEVSYKPLNRKYGIPKPKAVALIDEEYCIGCTFCIQACPVD
                     AIIGAAKCMHTVLTEQCTGCERCIAPCPVDCIRMVPIALTKRDEDPTLQRLAADKARE
                     RYQNRLRRLESERQRKNQLKSKSSTKVAQLASVDENLKKTMILAALERAKALSTVSLD
                     SNRGESEV"
     gene            complement(10947..11972)
                     /locus_tag="PKZ49_06075"
     CDS             complement(10947..11972)
                     /locus_tag="PKZ49_06075"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041353331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HND36164.1"
                     /translation="MLYRLLRPLLFTLDAELAHSLTLKALRLLQPFGLSDNRVVPCES
                     KTIMGLKFPNPVGLAAGLDKNGDYIDVLAKLGFGFIEVGTVTPRPQPGNPKPRLFRIP
                     EAHAIINRMGFNNLGVDYLLENIKRTPYHGILGINIGKNFDTPVESAIEDYLLCFRKV
                     YAVASYVTINISSPNTLNLRQLQNEHALDHLVATLKLEQSKLSDQQGRYTPLLVKIAP
                     DLDESQLESIAAILMKHNIDGVIATNTTISRPNIAGLSHADETGGLSGSPVREASTRV
                     ISKLYSLLGEQIPIIGVGGIVSANDAKEKIAAGASLIQLYTGLIYAGPDLVNQCISTL
                     CHSQHVE"
     gene            complement(12023..12745)
                     /locus_tag="PKZ49_06080"
     CDS             complement(12023..12745)
                     /locus_tag="PKZ49_06080"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HND36165.1"
                     /translation="MKLHVTESYPCSYLPDQLARSEIAVPDSRVVDTQAYAKLIQEGF
                     RRSGYFVYRPNCEHCTACVPVRIVVDQFVPNRSQRRAWKRHHTLRVSLHKLHFHPDHF
                     RLYRRYQQKRHALSEMSCDTHDQYCEFLLQSSIDSVLVTFHDGNRLQMVSIIDQLPDG
                     LSSVYTFFDPEIPQASLGTFSILWQIHYCRLEKHPYLYLGYWIEESRKMNYKAHFQPL
                     QYLRQGCWSRTAEPILKNRSVS"
BASE COUNT         3813 a         2813 c         2696 g         3505 t
ORIGIN      
        1 atctataaat tcagaaataa gattaactaa aaatacctac ttcaattcat ctctaccata
       61 aatagcgaga tagtttagaa ttaatctcga tttccttatc gaaggattaa acaggtcata
      121 gcgtaatgaa atatagttta ctgcatggtt catgccttct ttgatactct gaatgcgact
      181 tacgctattt ccatgcgatc cccaatactc aatttccatt aaggcagaat caatcatgca
      241 aaaaaaaatt tatcttgtag ccggtgcacg gcccaatttc atgaaaattg cacctatcat
      301 ccgtgcgctc caagagaaag agactttagc ctaccgcatt gttcatactg gccaacatta
      361 tgatcgagaa atgaacgaag tattctttga agaactcggc attccagaac ccgatatttt
      421 tatggctgct ggaggaggca gtcattccca gcaaacagcc aaaataatgc tagcttttga
      481 agaatattgc cttaacgata gacccgatgc ggtcattgtg gtgggagatg tcaattctac
      541 gcttgcttgc tccattgtag ccaagaaatt acatatcccg gtagcgcatg tcgaggccgg
      601 gcttcgtagc ggtgatttaa caatgccaga agaaatcaat cgactcgtta ctgatagcat
      661 ctcagattgg ttttttgtaa ctgaacccag tggggtagaa catctaaaac gtgaaggcaa
      721 agctgatgac gccattttct ttgttggcca tgtgatggtg gataatttgc tataccaagc
      781 cagtaaatta acgcatatca atctcgaaga atctgaaata agcaaattta agctagaccg
      841 gactctgagc aagcagcgct acggtgttat tacacttcat cgaccgagta atgttgatga
      901 tagagaagtt ttctctcgaa tcggaaaagc gctaaaagaa atttccaccc atttaccgct
      961 catttttcct actcatccaa gaactcggga aaatattaaa aaatttggta ttgaccttgg
     1021 tagcaacatc acgttagtag gccctcaagc ctatatgcca tttcttgatt tatggaaaaa
     1081 cgctgcagta gttctaaccg atagcggcgg cttacaagaa gaaaccactg cactagggat
     1141 tccttgcatt accattcgtg aaaatacgga acgtcctatt actgtggatg aagggacgaa
     1201 taccttagtt ggaaccaatc ccgagcgaat cattcaagaa gtagataacc ttctgcaagg
     1261 aaaaggtaaa caaggtcatc gacctcaact atgggatggt cacgctgccg aacgaattgt
     1321 tgagattctt gagaagaagc tcttgcagcg ggtctaaaag aacattcatt gcagtaaaaa
     1381 tattttttat aatttttttg tacaaaaact atgcactcat tgattcctgt tattttatct
     1441 ggtggctctg gaactcgact gtggcctctc tcgcgcgaaa agtatccaaa acaattattg
     1501 cccttgatca atgaagactc attgttacaa gccaccatca agcgtttaga tggtttaaca
     1561 ggcataaccg ttgaaaatcc actgatcgtt tgcaatgagg agtaccgttt tgtaattgct
     1621 gaacaactca ggctaatcgg aaaagcatgt tcaattttat tagagccatt tggacgaaac
     1681 actgctccag cactcactct tgcagcctta gtcgctgcac aaaacaatgc agatccgatt
     1741 ttactggtca tgccatccga tcatgtcatc gttaacgctt cagcgtttca agcatgtatt
     1801 agcaaaggcg tatccagtgc attacaaaat tcgattgtca cgtttgggat tgctcccgat
     1861 gccccagaaa ctggttatgg ctacattcaa acaggtgagt tgcttggaga cgtaggaata
     1921 actcaaatcg cccgcttcgt tgaaaaacca aaccaacaaa cagcccaagc ctatatcgac
     1981 gatggctctt atctatggaa cagcgggata ttcatggtcc gcgcatcagt ttggttagcc
     2041 gcgattaatc aatgtcgccc agatatcttt tcaacatgcc aaacagcatg gaataaaggt
     2101 tctcacgaag ggaatttcat tcgtgtcaat gaaacagcat ttactgcttg tcccaatgat
     2161 tccatcgatt atgcggtcat ggaaaagcta tcaagcaatc acccttcatt accatccggt
     2221 gtcgtgatcg aattctcagg aggttggtct gatgttgggg catgggattc cctttggcaa
     2281 gtgcttccta aagacgaagc gggcaacgtt tcacgcggag atgttatttt gcacgagtgc
     2341 aaaaatacac tcgctatttc agaaaatcgt ttagtggctt gcgtaggtgt tgaaaactta
     2401 actgtcgtcg aaacaccgga tgcaatctta gtggttcata aaaatagaac acaggacgtc
     2461 aagaaaattg tcgactcgct taaactaaca aaacgcgtag aagttatatt acaccggaaa
     2521 attttccgac cttggggttg gtatgatgcc gtggatgctg gagaacgctt tcaagttaag
     2581 cgtatcgtgg tcaagcctgg tgctgcctta tcccttcaga tgcatcatca tcgtgcagaa
     2641 cattggatcg ttgtccgtgg aacggcaaag gtaacacggg acgataaaag ttacttagtg
     2701 agtgaaaacg aatcaacgta tatcccgctg ggaacacgcc atcgtctgga aaatccaggt
     2761 cgactcccac tagaaatgat tgaagttcaa tcaggatcct acttaggtga ggacgatatt
     2821 gtccggttcg aagacattta tggtagaaat gaagcttata aacctaacta aaaaaacaga
     2881 gaaatgctgc ttctctaatt caaatctaca aatcgatttt atttacagat ttaagaacga
     2941 aaggacaaat gggttgaagt ataatatgcg tttttttcca tttcattctg tcgcgtaatg
     3001 gagatcagtc atgccaattt atgaatatat ttgccgttcc tgcggttttg aaaaagagca
     3061 cttacagaaa atgagtgatg caccaattgc acaatgtcca gaatgtggta gtcatgaata
     3121 cgttaaatta gtatcagcag cagggtttca attaaaagga agtggctggt atgttacaga
     3181 ttttaggaat aaacccactt ctaaatccga aactcaaaca aaacccaata cgagtgctgg
     3241 ggataatact actaccacat ctgaaaaaac tgcaaccccg acttcttcta ccggaactgc
     3301 agagtagtac aatacaactg atgtttataa cgagaactcg cctaaatcag ttttaatttt
     3361 tattgaatcc atgacattaa tatcgataca gtctgagcac gcaaaatttc tgtagaattg
     3421 cacgcgatta tcctgtaccc cttttttcta ttcgagccta attaaaatct atgcggacta
     3481 actattgcgg tgccatcgat aaaacctatt tagatcagga aattacactt tatggatggg
     3541 tgcatcgccg tcgtgatcac ggtggtgtta ttttcatcga tctacgtgat cgcgaaggca
     3601 ttgtgcaaat cgtatgcgat cctgatcaca aagaaaattt tcaaatcgct gagaaaatcc
     3661 gtaccgagta tgtccttaag atcgtagggt tagtaaggca tcgaccagca ggtacaacta
     3721 attctggaat tttttcgggt gaaatcgaag ttttagcgcg cgcaatcgag atactcaatg
     3781 catctctcac cccccctttc caaatggatg aagaaaatct cagtgaagcc attcggcttg
     3841 agtatcgcta ccttgacttg cgacgcccct cgatgcaaca gaatataagg ctaaggcatc
     3901 gcgtctcaat ggctatccgc acatttctgg atcaagctgg tttcatcgat gttgaaacac
     3961 ccatcctaac caaatcgacc ccagaaggcg cacgtgatta tttagtacct tcacgcgtcc
     4021 atgatgggca cttttttgct ttaccccaat cacctcagtt atttaagcaa ctgctaatgg
     4081 tatccggatt tgatcgctat tatcagatta cccgttgctt tcgtgatgaa gatttaaggg
     4141 ctgatcgcca acccgaattt acacaagtcg atatcgaaac ctcgtttctc tcagagaatg
     4201 agattatgga tttaatggaa aagatgattc gtcacttatt cctgaacgct atcgatattg
     4261 acctaccgaa ccctttccca cggatgactt ttgcagaagc gatgcataag tatggctccg
     4321 ataaacctga tttacgcgta ccgcttgtgc tcaccgaatt aacggacctg atgcaagaag
     4381 tcacttttca agtatttcgc gatgctgccc aaaaatcagg tgggcgtgtc gcggcccttc
     4441 gaattccaca gggcggagaa ttatcgcgta aagagattga tgactataca caatttgtag
     4501 cgatttatgg tgcaaaaggc ttagcgtata tcaaagtcaa cgatcgaaac aaaggtctcg
     4561 aaggtttgca atcaccgatt ctaaaattct tgccagaaac agtcattcaa gcaattttgg
     4621 aacgcacgca agcacaaaat ggggatctca ttttctttgg cgcggacaaa gctaaaatcg
     4681 tcaatgatgc gctcggcgca ttgcgtgtaa aaattgggca tgagcgtgga ttagcagaga
     4741 aaaattggaa acccctatgg gtcattgatt tcccaatgtt tgaatgggat gaagaagaaa
     4801 aacggtggaa agccctacat catccgttca catcccccat tgaaggccat gagaatctcc
     4861 tcactaacga tcctgagaaa gcactctcga gagcctatga tatggtatta aatggagtcg
     4921 aaattggtgg cgggtcagta cggattcatc gccaagaagt acaagcaaaa gttttccacg
     4981 cactcggaat aacagagcat gaagctcaag aaaaatttgg attcctactc aatgcacttc
     5041 aatacggcgc acctccacat gggggtattg catttggttt ggatcgcatt gtcgccatga
     5101 tgaccgggac cgaatccatt cgtgatgtga tcgctttccc taaaacacaa cgcgcacaat
     5161 gtatgctaac ccaagcaccg aatagtgtta ccgaaaaaca attacgggaa ttacacatac
     5221 gactccgtaa cccaagcgct agtatttctt aaattatcct tatagtgcat gcctataagc
     5281 ttcccgtatc agtattagtg gtcgtctata caatcgatcg tgacgttctt ctactggaac
     5341 gtgcagatca ccccggttat tggcaatcga ttaccggaag ccaagatccg gatgagtcac
     5401 tttggcaaac agcagttcga gaaattaaag aagagacggg attagttgcc aatcgatttg
     5461 aattaaacga ttggcacatc caaaatcagt atgaaatctt tcaagaatgg cgttggcggt
     5521 atgcccccgg agtaactcac aataccgaac atgtttttgg tttaaaatta ccagaaatta
     5581 tgcctatttc gattgcatcg cgcgaacacc tctcattgat ctggttaccc tggcacgaag
     5641 ccgctgaaaa agtgttttca tggagtaacg ctgaagcaat cagaaaactg gataccactt
     5701 aatcagttaa atgaatagct gcaaattcat gcatcccttg tctgtcgcat gcaagtcaaa
     5761 tttaaacaaa acatattgaa atttagcgct tattcttgat ttgggattag ttattgctgc
     5821 aatatcatat ttaggattat gaaaaggaaa cgattgtgag tgatttagac atagtcatat
     5881 tagctgccgg ggttggcaaa cgcatgcgat catctcttcc aaaggtgctc cataaattag
     5941 ctggtaaacc cttactcaag catgcaatcg acacagcgca aacattatcg cctagaaaaa
     6001 tttatatcgt atatggccat gggggcgaac aggttctgac tgaaatcaac gatgatcagc
     6061 tcacatgggt taagcaagaa ccacagcttg gcaccggcca tgctttgcta caagtgcttc
     6121 cttttttagc aaaatcaggt aaaaccttag ttctgctagg tgacgttcct ttaatacaag
     6181 aaaattcatt aactcagctt gttgcaagcg caagtgccaa caattttgca ttactaacag
     6241 tcacgttagc caatcctttt ggttatgggc ggattattcg tgaagatgga accaatcaag
     6301 tttcagccat tgtagaagag aaggatgcga acgaaataca gcgtagcatt aatgaaatca
     6361 ataccggtat tatggtactt cccaatgcct atctccatga ttacttaccc aaacttagta
     6421 atagcaatgc tcaaggtgaa tactatctga cggatatcat taagttagct gcgagttctg
     6481 gaattaagat tgaaaccgct caacctcact atgaatggga agtatccggt atcaatagta
     6541 aagtacaact cgcccagctt gagcgggcag cacaaaattt ccaagcgcaa caactattag
     6601 acgctggagt aacactcatg gatccgtctc gtattgatat tagaggcaac ctaatttgtg
     6661 gtaataatgt tgaaattgat gttaactgta tctttgaagg tacagttaca ttaggagata
     6721 atataaaaat caatgcaaac tgcatcatta gagatactat tatcagttca ggaacaactg
     6781 ttcttccgtt tagcctgata gataatgctg aaattggaaa aaattgccat attggaccct
     6841 atgcgcgaat aagaccaggc actcggttgg atgataatgt ccatatcggt aattttgtcg
     6901 aaataaaaaa cagttacatt gcaatggaca gtaaagcgaa tcaccttagc tatattggtg
     6961 atgcgactat cggcaaaaaa gtaaatattg gcgcgggaac aattacttgc aattacgatg
     7021 gtgcctataa acatcagacg atcattgaag ataatgtttt tgttggctcg gatacgcagt
     7081 taattgcacc ggttaaagtt gctaaaggct ctacgattgg tgcgggctcc acgatcacgc
     7141 gagacacgcc tgaagaacaa ctcacactgt ctcgcgctaa acaaatcagc gttgcagggt
     7201 ggaagcgccc aagtaaaact aaagtttaat aatactcatt gtcctggtaa atcgaagcaa
     7261 ttggaaggag aaaagcatgt gtggaattgt aggcgcagtc gcgaaagata atgtggttcc
     7321 tattctgcta gaaggtcttt ctaggctcga atatagaggc tatgactcgg ctggcattgt
     7381 cgttgcaaat gcagaattac aaagattacg cactaccgaa cgcgtttcag aacttggaag
     7441 actcgttgca accaaaaaaa cccaaggact taccggaatt gcacatacac ggtgggcgac
     7501 gcatggtgca ccttcagaaa gaaatgctca cccacatttt tcagggcaag ataaaaaaat
     7561 tgcggtcgtg cacaatggta tcatcgaaaa tcatgagcaa atccgcaagc gccttgaaaa
     7621 agaaggtttc gagttcctat ccgatacgga tactgaagtg attgctcacc tcatatcctt
     7681 caacctcgaa acaaccccaa atctgcttga agcagtatgt aagacaatca atgagctaca
     7741 aggtgcgtat gcaattgcgg ttgtggaaga atcgcaacca gatcacatcg tcgtagccag
     7801 aaatggcgca ccgctattat taggtgtcgg taacaatgga aactatgtcg cttcggatgc
     7861 ctctgcgctc attcaagtga ctaaacatat gatttacctt gaagaaggcg atgtagcaga
     7921 attacagagc aatggttacc gcgttcttaa ccgtattggc aacgctgcct ataaagaagt
     7981 aacacgttta gtccgagaaa gcgatttaac gcgtgattct gttgaattag ggccttacgc
     8041 ccattacatg caaaaagaaa tctttgagca accgatcgcg gttgctaata ccctggaaat
     8101 ggtattaaac gcccattcta tttcacctca actctttggg actgaagcag aagccatttt
     8161 caagaaaacc aatggtattc taattcttgc ctgcgggacg agctatcatg caggcttagt
     8221 tgccaaatac tggctagaaa cgatcgctgg gttaccttgt aatgttgaaa ttgccagtga
     8281 gtaccgttat cgcgatccca ttgcaaaccc agattgcctg gtcatcggta tttcccaatc
     8341 aggggaaacg gcagacacgc ttgccgcgat caattacgca aaaactcttg gacataaaca
     8401 tagcttgtcg atttgtaatg ttccagaaag cgctttgatc agacaaacca atttacgctt
     8461 tttaacacgt gccggccctg aaattggtgt ggcttctaca aaagcattta caacgcagtt
     8521 agcagcactc atgctattaa cggtgacact cgcaaagctt agaaatcggt tttctggaga
     8581 gctagagcag gaagtcatta cttcattacg ccacctccca attgccattc aatccgcgtt
     8641 atctgtcgaa ccccaagtta aagcatgggc cgccaatttt tcacaaaaac gacatgcgct
     8701 atttctgggc cgcggcgttc actatcccat tgcattagaa ggcgcactta aattaaaaga
     8761 aatttcttat attcatgcag aggcttatgc agctggtgaa ttaaaacatg gcccgctagc
     8821 gttagttgat aaggaaatgc ccgtcattgc catcgcaccc aatgatgcat tattagaaaa
     8881 acttaaatcc aacttacaag aagttcgcgc acgcggtggc gaactctatg tgtttgctga
     8941 tagcgattcg catattcaag aaagtgaagg gattcatatt atccggcttg cggagcatgc
     9001 aggtatgtta agtcccctgt tacataccat tccactacag ttattagcgt accatgttgc
     9061 tttacagaaa gggaccgatg tcgataagcc aaggaactta gcaaaagcag ttactgttga
     9121 ataatcatgt ggctgatcag ttctatgtaa tggttttttt agttcatatt ttggactagt
     9181 actttcttaa gacctaatgg ctttatcaag gcaatctaaa taaatctgcg cattagggct
     9241 cgtttgatcc cgttgcgcat gccaaagcat ctcacccaag caatccatta cgttatggat
     9301 tgccgcatgt tcatcgccta atttcacgac tagattgcga tatctttcct ggataccaac
     9361 cggttgattg atagaaagct gttctttgat tgccatatgc atgctcatgt gcaagaacgg
     9421 atttgtctct ccaaactccg gtagatagtc cttgtcgatg aattgatcgt aatcttcgag
     9481 tatcggatga tattctgtgt gttgtaatat cacttccagt gcaattgttt ccattccgga
     9541 taatagctca tgtcggccat atttatacca agtgtcaaag aataattgcc gggcttgatt
     9601 tcgagtggga ttaaacattg ctacgattca aggtctcgct tcgttaaata taaatttttc
     9661 tcgtcaaatt cgcataaatc gctgatgcag cattcgtggc aagcaggctt cctggcctta
     9721 cacgtatatc gcccatgtaa aatcaaccaa tgatgggcat cgtgttggaa aggagcaggc
     9781 actcgcttca gtaacttatt ctccacttcc agcacatttt ttcctggtgc tagtccagtt
     9841 cggttcgcga ccctgaaaat atgagtatct actgcaatgg ttggttctcc aaatgcagta
     9901 ttcagcacca cattcgccgt tttacggcca accccaggaa gctgctcaag gtcttctcga
     9961 tttcgcggta cttgaccctc atataagtca tttaacaact tacacgtcgc taggatattt
    10021 tttgctttgg ttcgatataa tccaatccgc tggatcattt cacttaatcg cacctcaccc
    10081 agtgcaagaa tttttgctgg ggtatttgcc acgagaaaaa gtttttgcgt tgcaagattg
    10141 acacttttat ctgttgcttg cgccgaaaga atgaccgcga ctaataattc gaatggtgtt
    10201 gaataaacca actctgtcgt tgggtggggg tttaacgact tcaatcggct aaacatttct
    10261 tggcgcttcg cagcattcat acttcagact caccccgatt gctgtccagg ctaacagtcg
    10321 ataaggcctt agcacgttct aatgccgcaa gtatcattgt tttctttaga ttctcatcga
    10381 ctgaggctaa ttgagccacc ttggtcgatg atttactttt aagctgattc ttgcgctgcc
    10441 gttcggactc taatcgtcgc aatcgatttt gataccgctc tctggcttta tcagccgcca
    10501 atcgctgcaa tgttggatcc tcatctcgtt tagtcagcgc aatgggaacc atccggatac
    10561 agtctacagg gcaaggcgcg atacaacgct cacacccagt acattgctca gtaagaacgg
    10621 tatgcatgca tttagctgct ccaatgattg catccacggg acaagcttga atgcaaaaag
    10681 tgcaaccaat acagtattct tcatcgatca aagcaaccgc tttaggcttc ggtataccat
    10741 actttctatt cagtggttta taggaaacct ctaataacac agccaattga tgaataactg
    10801 tctcatcacc gggaggacat tgattaatat ccgcaacacc ttgcgccatt gccgttgcat
    10861 aaggcaggca acctgaataa ccacattgtc cacattgtgt ctgcggcaaa atcgcatcga
    10921 tttgtctgat taatgaatcg ttttcgttac tcgacatgtt gcgaatgaca aagtgtactg
    10981 atgcactggt ttaccaaatc agggcctgca tagatcaaac ccgtatagag ttgaatgaga
    11041 cttgcgcccg ctgctatttt ttctttggca tcattggccg acacaatacc tccgacacca
    11101 atgatcggaa tctgctcccc tagcagcgaa tacaatttac taatgactcg ggtactcgcc
    11161 tccctcactg gggatccact caatcccccg gtttcatccg catgcgataa ccctgcgatg
    11221 ttcggacgcg aaatcgtagt gttggtggca ataacaccgt caatattatg tttcattaag
    11281 atagcagcaa tcgattcaag ttgtgattca tccaagtcag gggctatttt caccaatagc
    11341 ggcgtgtagc gcccctgctg atcactgagc ttactttgct ctaattttag cgtagcaact
    11401 aaatgatcta acgcatgctc attctgcaat tgcctcaaat tcagtgtatt aggggaagaa
    11461 atattgatcg ttacatagct agcaaccgcg tataccttac gaaaacagag taaataatct
    11521 tctatggcgc tttcaaccgg ggtatcaaaa ttttttccga tattgatacc cagaattcca
    11581 tgatatggag tgcgctttat attctctaac aaataatcga caccaaggtt gttaaagccc
    11641 atacgattga tgatagcgtg cgcttctggg atgcgaaata gccgaggttt tggattgcca
    11701 ggttggggtc gcggcgttac tgtgccaact tcaataaagc caaaccccag tttagccagc
    11761 acatcgatat aatccccatt cttatccaga ccagccgcaa gccccactgg attagggaat
    11821 ttgagtccca taatggtttt agactcacag ggaaccaccc gattatctga taacccgaat
    11881 ggctgcaata aacgcaatgc tttaagcgta aggctatgcg ctaattcagc atcgagtgta
    11941 aagagcaaag ggcgcaacaa acgataaagc atgaagatga tttcctgggt tcaagcgaaa
    12001 atttagcgga ccatcatgat aattaagata cagatcgatt cttcaatata ggttcagccg
    12061 tacgtgacca gcacccttgt cgcaaatatt gcaacggctg aaaatgcgct ttgtaattca
    12121 ttttacggct ttcctcgatc caatagccta aataaagata agggtgcttt tctaaccggc
    12181 aatagtgaat ctgccacaaa atgctaaaag tacccaagct tgcctggggt atttctggat
    12241 caaaaaaagt ataaactgaa gataaaccat cgggtaactg atcaatgata ctgaccatct
    12301 gtagtcggtt tccatcatga aatgttacaa gcactgagtc aatactgctc tgcaacaaaa
    12361 actcacaata ttggtcatga gtatcacagc tcatttcact gagggcatgg cgcttttgct
    12421 gataacgacg atagagccgg aaatggtcag ggtggaaatg caacttatgc aagctcaccc
    12481 gtaaagtatg atggcgtttc caagcgcgac gctgagagcg gttaggcaca aattgatcga
    12541 cgacaatgcg caccggcaca caggccgtac agtgctcgca attgggtcga taaacaaaat
    12601 acccactgcg acgaaacccc tcttgaatca acttcgcata agcctgtgta tcgactaccc
    12661 gactatctgg aaccgcgatt tcagagcgtg ccaactggtc tggcagataa ctgcagggat
    12721 aggactctgt aacgtggagc ttcattattt gaaaaaacga tcaaccagga taagtataat
    12781 caaaatgcca tgtccctgat cgatccgaaa tatcgatcag cttactt
//
LOCUS       DAOQKQ010000069        12664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_2327_length_12664_cov_7.042351, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000069 DAOQKQ010000000
VERSION     DAOQKQ010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 12664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12664
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2327_length_12664_cov_7.042351"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..1139
                     /gene="ilvC"
                     /locus_tag="PKZ49_06085"
     CDS             123..1139
                     /gene="ilvC"
                     /locus_tag="PKZ49_06085"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HND36166.1"
                     /translation="MKVYYDKDADLSLIKKKKVTIIGYGSQGHAHANNLRDSGVKVTV
                     GLRKDGASWDKAKKAGFTVKEFAASIKDADVVMLLLPDEQIASIYKTEIEAGLKKNAT
                     LAFAHGFNIHYGQVVPREDLDVIMIAPKGPGHLVRSTYTQGGGVPSLIAIHQNKSGKA
                     RELALSYAAANGGTKGGVIETTFREETETDLFGEQVVLCGGLTALIQAGFETLVEAGY
                     APEMAYFECLHEVKLIVDLIYEGGIANMRYSISNNAEYGDISRGPRIIDDKTRAEMRK
                     ILREIQTGEYAREFILENRAGAPMLKSSRRIASEHPIEVVGTRLRDMMPWIKKNKLVD
                     QVKN"
     gene            1254..1907
                     /locus_tag="PKZ49_06090"
     CDS             1254..1907
                     /locus_tag="PKZ49_06090"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HND36167.1"
                     /translation="MKPNYPHPILAREGWPHIAIALLIAASVLVLANWQWSIPFWLLA
                     LFVIQFFRDPARAIPSQGNAILSPADGRIVAVEQTEDPYLKREAIKISVFMNVFNVHS
                     NRSPITGKICEKWYFPGSFLNASLPKASLENERNALWIKTKQGIDVTCVQIAGLVAKR
                     IVCHVKPGDELSAGQRFGFIRFGSRVDVYLPTDIKACVNIGDKVYATTTILAEFKSS"
     gene            2007..2789
                     /gene="pssA"
                     /locus_tag="PKZ49_06095"
     CDS             2007..2789
                     /gene="pssA"
                     /locus_tag="PKZ49_06095"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HND36168.1"
                     /translation="MSEPNLTRFGIRSRLRRRGIYLLPNLFTTAALFAGFYAIVQAMH
                     GNYEYSSVAIFIAMVLDGLDGRVARLTHTQSEFGAEYDSLSDMVSFGVAPALVIYEWA
                     LRDMDKLGWIAAFIYCACAALRLARFNTNAEVIDKRFFQGLPSPAAAALVAGLIWVAS
                     DFQVQGTDIKWIAWSITLFAGLTMVSNLPFYSGKEINLRKRMPFFTILLLLMFFFVLI
                     PSHPPVVLFGLFSVYALSGYFMKLWLFSKNKKKSETQEQDVT"
     gene            3028..4560
                     /locus_tag="PKZ49_06100"
     CDS             3028..4560
                     /locus_tag="PKZ49_06100"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HND36169.1"
                     /translation="MQDRLIIFDTTLRDGEQSPGASMTKEEKIRIARQLEKMGVDVIE
                     AGFPAASNGDFEAVKAVASNVKDSTVCGLARAIESDIQRAGEALSGASAARIHTFIAT
                     SPIHMKNKLRMSPEQVIEQAVKAIKWASAYTDNIEFSPEDAGRSEIDFLCRVLEAVIN
                     AGAKTLNIPDTVGYTMPEQFGRLIRTLRERIPNSDKVIFSVHCHNDLGLAVANSLAAV
                     SNGARQVECTINGLGERAGNAALEEVVMAVRTRQDYFSCDTRIDTTHIVQASKLVSGI
                     TGFPVQPNKAIVGANAFAHESGIHQDGVLKHRETYEIMKAEDVGWNANKLLLGKHSGR
                     NAFRTRLNELGIELESEEMLNAMFMRFKELADKKHEIFDEDLHALMSDEAHIPEERYR
                     LLSLMAHSETGEPPFARLVMANGSDELHTESDGSGPVDATFRAIEKILKSGAELQLYS
                     VNNITSGTDAQGEVTVRLQKSGRIVNGHGADTDIVVASAKAYLSAINKLYSKLERAHP
                     QV"
     gene            4566..5030
                     /locus_tag="PKZ49_06105"
     CDS             4566..5030
                     /locus_tag="PKZ49_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HND36170.1"
                     /translation="MKHLLCYLFIGLCFITNVIHAEEFAFRFQDSTGKVHKLSDYKGK
                     WVLVNFWATWCPPCLEEIPDLVTLYENRSDIMVFGVAMEYSEVKTVMEFAKALSVTYP
                     IILGNRVIAAQFDELSMLPSTYLFDPKGNPAARKVGLLTRADIEAFIQNYPK"
     gene            5301..8039
                     /gene="secA"
                     /locus_tag="PKZ49_06110"
     CDS             5301..8039
                     /gene="secA"
                     /locus_tag="PKZ49_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HND36171.1"
                     /translation="MFSNLLKKIFGSRNDRLVKQYTQVVRAINGLESTIAALSDQDLV
                     NKTQAFKEQIANGQSLDELLPEAFAVVREAGKRVVKMRHFDVQLIGGMVLHDSKIAEM
                     RTGEGKTLMATLPAYLNALSGQGVHIVTVNDYLARRDAEWMGQIYRFLGLSVGVVMSQ
                     MPHELKQQAYAADITYGTNNEYGFDYLRDNMVGHPAERVQRVLNFAIVDEVDSILIDE
                     ARTPLIISGQAEGDTDIYIRINRLIPKLTRQENEKSPGDYSVDEKAQQVLLSEAGFEH
                     AEKLLSDAGLLANDTSLYDPININLIHHLNAGLRAHALFTRDQHYVVQNGEVIIVDEF
                     TGRLMPGRRWSEGLHQAVEAKENVSIQKENQTLASITFQNYFRMYQRLSGMTGTADTE
                     AFEFQQIYGLETVVIPTHNPMIRDDRMDKVYRTMKEKYQAVLEDIKECYQRGQPTLVG
                     TTSIENNELLSSLLNKENLPHQLLNAKQHAREADIVAQAGQPKMITIATNMAGRGTDI
                     VLGGNPEPEINRIRMDESLDEPTKAKQIEEIRKVWQARHEEVVKLGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLALEDPLLRIFASDRVANIMTRLKMPEGEAIEHPW
                     VTRAIENAQKKVEARNFDIRKQLLEFDDVANDQRKVIYQQRNELLEAPQDIGETITAV
                     RESILTSLFNRHIPPESIEEQWDIPGLEKELASDYHLTLPIHEWIEKEDDLQSEDILE
                     RILEIAAQHYQQKSELVGNHIIHQYERALMLHSLDNHWREHLSALDHLRQGIHLRGYA
                     QKNPKQEYKREAFELFGSMLEAIKAEVTKILLTVQIKNEQQVETASEVHTPVNVQYQH
                     ATYEDELTQEQQATPEKASQENKTKPFVRKMDKVGRNDPCPCGSGKKYKQCHGKLK"
     gene            complement(8070..9614)
                     /locus_tag="PKZ49_06115"
     CDS             complement(8070..9614)
                     /locus_tag="PKZ49_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HND36172.1"
                     /translation="MNEAIKQSLRQAQEMIRSGDLNGASEILEPLKQTYPELTDVAQL
                     WCAMMMRAGRSGEVLPYAAKIFSLAEGDLQKARWAQMMGTACFQLLELETACYHYMTA
                     LNHLMALTKSGKVPADQKSRTVKESTENIFLSGRAERLLWETCAALAANGIRAFPFAG
                     TLLGIVRHGHLLAFDKDLDIAVWMENWDDCCALLEKMGWQRTPMSFNYRNYRDYVHSE
                     IGITLDLCGLQLNKANRIIGGFSLPGYPDEYQRVSIFPKFDLIPKDTPYGTVWFPNPP
                     ETILTAFYGNWRTPNPYWDTVVSAVNLEKFTLLVRCFAYYRLIQRWLSGELVRAWSYA
                     HQIALKDPDDVISLRSRQWLERALTRLNQAIPEWPSNRSQKRVYTRMVADLFHVGHVN
                     FLREAKALGTHLTVFVVSDQRVQENKGKHPVMSQSERAAVVSACKYVDAVVTEGPLYA
                     TPAFMQQQGYDLYTFACSSEKERQEKYKLCAELPASMIKEIDYTDGISTTDLLTRITI
                     QDRNVS"
     gene            9752..10366
                     /locus_tag="PKZ49_06120"
     CDS             9752..10366
                     /locus_tag="PKZ49_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulating protein"
                     /protein_id="HND36173.1"
                     /translation="MAITKEPRPNLSNSGNIFEIIDGIQFLGFGSGGFDANDLLIIGD
                     ELDNLLEGNNGADDHDEIEGGAGNDRLLGLNGNDTLDGDEGNDDLNGGEGTDKLFGGL
                     GDDILRAGFGHDRLTGNDGSDTFGFYAAGFYQVKDFSITQNDRLFFDSQTLGINSLEQ
                     LVTYITNIEQRADGFKVYFGEVATIDIVGANLSAITADMIVFNL"
     gene            10521..10706
                     /locus_tag="PKZ49_06125"
     CDS             10521..10706
                     /locus_tag="PKZ49_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HND36174.1"
                     /translation="MYHSYMHLQTSNNSDHSAANDLRKSINSDALFTGNNEIVIQHRE
                     QQYRLRLTRAGKLILTK"
     gene            10898..11503
                     /locus_tag="PKZ49_06130"
     CDS             10898..11503
                     /locus_tag="PKZ49_06130"
                     /inference="COORDINATES: protein motif:HMM:NF021593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HND36175.1"
                     /translation="MKLLKFPLSQTIYCDNSHNWVNWQQISHITNTKLKNIQNWITVV
                     SAKIFRSHALRSSTPLTTRVIPLIKEKQINPRLLIFANYKPFNKTEGLAGKIILCIGG
                     RIALYSDYQKLVEIAGGNFIGYRGADMQSDQLKKLLDQANMVICPVDCINHEDFFFVK
                     NYCAITGKICTFLARSTLLNFQQGLKILINLSHHNLGTVVS"
     gene            11500..12486
                     /locus_tag="PKZ49_06135"
     CDS             11500..12486
                     /locus_tag="PKZ49_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HND36176.1"
                     /translation="MKNKKIDAVSLFIAFLLGVFFILSSQLEAKTIREYWIAAEKVTW
                     NYAPSGKNLIEPEGNLGVWGETLTYTKYQYVGYTDGSFNAPIAQPEWAGLLGPQIRAV
                     VGDTIKVHFLNKTDRPLSIHPHGIFYKKEDEGADGGKGSSVQPGERYTYTWIADEDAG
                     PGPSDPSSIVWLYHSHVMAEEETNLGLIGTLIITRKGKERSRDDPTPKDVTQEFIALY
                     TIFDEENGEEPGLKHAINGRIFGNLAGYQTQLGSRVRWYLVALGNEVDNHTVHWHGQT
                     VLHRGHRTDVIELMPASMTTVDMVPRSAGNWLFHCHVNDHMMAGMSTYWHVK"
BASE COUNT         3822 a         2595 c         2689 g         3558 t
ORIGIN      
        1 cgattagaac tggtgcgtca ggtctcggtc gtggagatcg tatgcttaaa ttgtaactaa
       61 agtaattttg cttcagtctt tttgtatttt tgatgctaga tattaataaa taaaggatca
      121 caatgaaagt ctattatgac aaggatgctg atttatccct gattaaaaaa aagaaagtaa
      181 cgattattgg ttatggctca caaggtcatg cgcatgccaa taatcttcgt gactcgggcg
      241 ttaaggtaac ggtagggtta cgtaaagatg gtgcttcctg ggataaagct aaaaaggcag
      301 gttttaccgt taaagaattc gctgcttcaa ttaaagatgc agacgtcgtc atgctgttgc
      361 tcccagatga gcaaatcgca tcgatttata aaaccgaaat tgaagcagga ttgaaaaaaa
      421 atgcaacgct tgcttttgcc catggcttca atattcacta tggtcaagtt gtaccacgag
      481 aagatctcga tgttatcatg attgcgccta aagggcctgg gcatcttgtc agatcaactt
      541 atacgcaagg tggaggagtg ccctctctta tcgcaattca tcaaaacaaa tcaggtaaag
      601 cacgcgaatt agcattatcg tatgctgctg ccaatggcgg gactaaaggt ggtgtaatcg
      661 aaaccacatt ccgtgaagag actgaaaccg atttatttgg agagcaagtt gtactatgtg
      721 gtggtcttac cgcattaatt caagctggat ttgaaacatt ggttgaagcg ggctatgctc
      781 cagaaatggc ttacttcgag tgcctccatg aggtcaagct cattgtagat ttaatttacg
      841 aaggtggtat cgccaacatg cgttattcga tttccaataa tgcagagtat ggtgatattt
      901 caagaggacc tcgaattatc gatgataaaa ctcgagctga aatgcgtaag attctccgtg
      961 agattcaaac cggtgaatac gctcgcgaat ttattctaga aaaccgagct ggcgcgccca
     1021 tgttaaaatc gagccgccgg atcgcttctg aacatccaat tgaggtcgtt ggaacgagac
     1081 tccgcgacat gatgccatgg atcaaaaaga acaaattagt cgatcaagta aaaaactaat
     1141 catcgctcat tcaatttatt gatagattta ctttttcact tgttttttcg cgcttgctaa
     1201 cccggtattc ttcacaatat cgggtttatt atttttagta tgatgaaatt gctatgaaac
     1261 caaattaccc tcaccctatt ctcgcccgcg aaggatggcc tcatattgct atcgcattac
     1321 tgattgcagc ttctgttctt gtcctagcta attggcaatg gtcaattcca ttttggttgc
     1381 ttgctttatt tgttatccag ttctttcgtg atccagcgag ggccattcct tctcagggaa
     1441 atgcgatatt atctcctgct gatggaagaa tcgtcgcagt agaacaaact gaggatcctt
     1501 atttaaagcg agaagcaatc aaaattagcg tattcatgaa tgtatttaac gtacattcaa
     1561 atagaagccc gattactggc aaaatttgcg aaaaatggta cttcccagga agctttctca
     1621 atgctagcct ccccaaggct tcacttgaaa atgaacgcaa cgccctctgg ataaaaacga
     1681 aacaagggat agatgtaact tgcgtacaaa ttgcagggtt agtcgcaaag cgtattgttt
     1741 gccatgttaa gccaggagat gaactctcag ctggacaacg ttttggattt attcgttttg
     1801 gctctcgagt tgatgtttat ttacccacag atataaaagc ttgcgttaat attggtgata
     1861 aagtatatgc aacaacaact atattagctg aatttaaatc atcataacta aaacgattag
     1921 ttgtaacctt tctagttgat actttaagaa tttattgatg ttttatattg atgttttatt
     1981 aggcgcttat cttgatgctg tgagcaatgt ctgaacccaa tcttactcga tttggtatta
     2041 ggtcaagact aagaagacgt ggaatttacc tattaccaaa tttattcacg acagctgcct
     2101 tattcgcggg attctatgcc attgtgcaag caatgcatgg caattatgag tattcctctg
     2161 tagctatttt tatcgcaatg gtactcgatg gtctagatgg tcgtgttgca cgactaacgc
     2221 atacgcaaag tgagtttggt gcggagtatg atagcttgtc ggatatggtt tcgttcgggg
     2281 tcgctccggc tctggtcata tacgaatggg cactccgtga tatggacaaa ctcgggtgga
     2341 ttgccgcttt tatctactgc gcctgcgctg cactaagact cgcacgcttc aataccaatg
     2401 cagaagtgat cgacaaacgc ttctttcaag ggttacctag tccagctgct gctgccttag
     2461 ttgccggatt aatttgggtc gcatcagatt ttcaagtaca agggactgat attaaatgga
     2521 ttgcatggtc tataacctta tttgctgggt taacaatggt cagtaatcta cctttctata
     2581 gcgggaaaga aattaatctg aggaaaagga tgcccttttt caccattcta cttttactca
     2641 tgtttttctt tgttctaatt ccaagccatc ctcccgtcgt attatttggc ttattctccg
     2701 tttatgcttt atctggctat tttatgaaat tgtggctttt tagcaaaaac aagaaaaaaa
     2761 gtgaaactca agaacaggat gtgacttagc cagtttttgt tgttgcagga atataaaaaa
     2821 cattttatat ttcagcacca tggaaaactt aattagtaag catttcgtaa acctgttgtt
     2881 ctctcctaat tctgtctcaa gaatagcttg gggctatcta ctgcgcttcg cgcgcaaata
     2941 attctaatac tttatacctt tcttgatttc cctaaaaaaa acgattctcg tttttttatt
     3001 taatcgtagt cctgtaggag aaaaataatg caagatcgtt taatcatatt cgacacgact
     3061 ttgcgcgatg gagaacaaag tcctggtgca tccatgacaa aagaggaaaa aattcgtatc
     3121 gctcgccaat tagaaaaaat gggggtcgat gtgattgaag caggttttcc tgctgcctcc
     3181 aatggggact ttgaagcggt taaggctgtc gcaagtaatg taaaagatag tactgtctgt
     3241 ggattggcac gcgcgattga atccgatatt cagcgcgctg gtgaggcatt aagtggtgca
     3301 agtgccgcgc gtatccatac cttcattgcg acttcgccca ttcatatgaa gaataaactg
     3361 cgtatgtctc cagaacaggt catagaacag gcagtaaagg cgataaagtg ggcaagtgcc
     3421 tacactgata acatcgaatt ttctccagaa gacgctggaa gatcagaaat tgattttctt
     3481 tgtagggtgt tagaagcagt catcaatgct ggtgctaaaa cattaaatat tcctgatacc
     3541 gttggctata ccatgcctga gcagtttggt cggttaattc gaacattgcg tgaacgcatt
     3601 ccaaactctg acaaagtcat cttttccgtt cattgccaca atgatttagg gcttgcagtg
     3661 gcaaactctt tagcagcagt atctaatggt gcaagacaag ttgaatgcac tatcaatgga
     3721 cttggtgagc gcgctggtaa tgcagcactt gaggaagttg tcatggccgt caggacacgt
     3781 caagactatt tttcatgtga tactcgtatt gatactacac atatcgttca ggcgagcaag
     3841 ttagtatccg gtatcacggg tttccccgta caaccgaaca aggccattgt tggcgccaat
     3901 gcattcgctc atgaatcggg tattcatcaa gatggtgtcc ttaaacatcg tgaaacctac
     3961 gaaattatga aagctgaaga tgttggctgg aatgccaaca aattgctgtt gggtaaacac
     4021 tcagggcgta atgcctttcg cacccgatta aacgaattag gtatcgagct tgagtcagaa
     4081 gaaatgttaa atgctatgtt catgcggttt aaagaactcg ctgataagaa acatgaaatc
     4141 tttgatgaag atctccatgc cttgatgtcg gatgaggctc atattccaga agagcgatat
     4201 cgcctgttat cattgatggc gcatagcgaa acaggagaac ctcctttcgc tcgattagtc
     4261 atggcgaatg gtagcgatga acttcatacc gaatctgatg gaagcggccc cgtcgatgca
     4321 acttttcgtg ctatagaaaa aattttgaaa agtggtgcgg agcttcagtt atattcagtt
     4381 aataacatta caagtggaac ggatgctcaa ggggaagtca ctgttcggct gcaaaaatcg
     4441 ggaagaatcg tcaatggaca cggtgcagat acggatatcg tcgtggcttc tgccaaagct
     4501 tacctcagtg cgatcaataa gctatacagc aaactagaaa gagctcatcc acaggtttaa
     4561 taaaaatgaa acacttactt tgctacctat ttatcgggct ctgttttatt actaatgtta
     4621 tccatgcgga agaatttgca ttccgatttc aggatagcac tggtaaagtc cataagctta
     4681 gcgattacaa aggtaagtgg gtactcgtaa atttttgggc tacttggtgt ccgccttgtt
     4741 tagaggagat tcccgattta gtgacacttt atgaaaatcg aagcgatatc atggtgtttg
     4801 gggtcgccat ggaatattct gaagtaaaaa cggtaatgga atttgcaaaa gcattatcgg
     4861 ttacctaccc catcatacta ggtaaccgag tcattgcagc tcaattcgat gagctttcaa
     4921 tgctgcccag cacttattta ttcgacccta aaggaaatcc agcggcgcgt aaagttggat
     4981 tgctaacacg cgctgatatc gaagctttta ttcagaacta ccccaaataa tccagaatgt
     5041 tattttctaa tactttctga aagtaggtta ctctaaaata ctggatcatg catcgtatca
     5101 tctaaaaact atcgattaga aagcagtgag ttagctcggg tatgttaaga tgttatttat
     5161 tcggcttcat tgggacctga ttcgtacaat ctcacgcgat acgatttctg gactaagccc
     5221 aagtaggtgt gcaattgaga caagactatt atttcttata atcactattt catcttaacc
     5281 agtaataaaa tttttacttt atgttcagta atctccttaa aaagattttt ggtagccgga
     5341 acgaccgttt agttaagcaa tatactcagg tagtacgtgc tatcaatgga ttagaatcga
     5401 cgattgccgc actcagtgac caagatctgg tcaacaaaac acaagcattc aaagagcaga
     5461 ttgcgaatgg gcaatccctc gatgagttgt tacctgaagc attcgctgtc gttcgagaag
     5521 ctggtaaacg tgtcgtaaaa atgcggcact tcgatgttca gctaattggc ggtatggtac
     5581 ttcatgattc taagatagca gagatgcgga caggtgaagg taaaaccctg atggcaactt
     5641 taccagccta cctaaatgcg ctttcaggac aaggtgtgca tatcgtaacc gtaaatgatt
     5701 atcttgccag acgtgacgct gaatggatgg gacaaattta tcgctttctg ggcttatcgg
     5761 ttggcgtagt catgtcacaa atgccacatg aattaaagca gcaagcttat gcagcagata
     5821 tcacttatgg aactaataat gaatatggtt ttgattattt acgggacaat atggttggcc
     5881 acccagccga gcgtgttcaa cgggtactga attttgcgat agtcgatgaa gtagattcga
     5941 ttttaatcga tgaagcacga acaccattga tcatctccgg acaagctgaa ggtgataccg
     6001 atatctacat ccgaatcaat cgcctaatac ccaaacttac tcgccaagaa aacgaaaaaa
     6061 gcccgggtga ctatagcgtt gatgaaaaag cacagcaagt actactcagt gaagctgggt
     6121 ttgagcacgc agaaaaatta ttatcagacg caggtttgct cgccaatgat acaagtttgt
     6181 atgatccgat caatatcaat ttgattcatc acttgaatgc gggattacgc gcacatgcac
     6241 tatttacccg tgatcagcat tatgtagttc agaatggtga agtcatcatc gtcgatgagt
     6301 ttacggggcg tctcatgcca ggtcggcgtt ggtccgaagg tctgcaccaa gccgttgaag
     6361 ccaaagaaaa tgtttcaatt cagaaagaaa atcaaacact cgcttcaata actttccaaa
     6421 actattttcg tatgtatcaa cgtttgtcag gaatgacagg aacagcagat actgaggcat
     6481 ttgaattcca gcagatttat ggcttagaaa ccgtggtaat ccctacacat aatccaatga
     6541 tacgggatga tcgcatggac aaagtgtacc gcaccatgaa agaaaaatac caagcggtac
     6601 ttgaagacat taaagaatgc tatcaacgcg gccagccgac gctagtcggt acaacctcga
     6661 ttgaaaataa tgagctgctt tcttcattac ttaacaaaga aaatcttccc catcaactcc
     6721 taaacgccaa acagcatgct cgggaagccg atatcgttgc acaagcgggt caaccaaaaa
     6781 tgattaccat cgcgaccaat atggcgggac gcggtactga tattgtgctc ggtggtaacc
     6841 ccgagccgga aatcaatcgt atccgaatgg atgaaagctt agatgaaccg acaaaggcaa
     6901 agcaaatcga agaaattcgg aaagtatggc aggcaagaca tgaagaagtc gtcaaattag
     6961 gtgggttaca tattattgga actgaacgcc atgaatcacg ccgtatcgat aatcagcttc
     7021 gtggccgttc tggacggcaa ggtgatcctg gctccagtcg attttatctc gcactagagg
     7081 atcccctcct acgaatattt gcctcggatc gtgtcgcaaa tattatgacg cgactcaaaa
     7141 tgcctgaagg cgaagcgatt gagcatccat gggttactcg cgcgattgaa aatgcgcaga
     7201 aaaaagtaga ggcaagaaat ttcgatatcc gtaagcaatt actcgaattc gatgatgtcg
     7261 ccaacgatca acgtaaagtc atctaccagc aaagaaatga gctcctagag gcgccgcagg
     7321 atattggtga aaccattact gcagttagag aaagtatatt aacaagcctg ttcaatcgcc
     7381 atattccacc cgagagcatt gaggagcaat gggatattcc agggttagaa aaagaactag
     7441 caagtgatta tcacttgacg ttacccatcc atgagtggat agaaaaagaa gacgatttac
     7501 aatctgaaga tattttagaa agaattttag aaatcgcagc gcaacattac cagcaaaaat
     7561 ctgagctagt aggtaatcac atcattcatc aatatgaacg ggcactaatg ctgcatagtc
     7621 tcgataatca ttggcgtgaa catttatccg ccttggatca tttgcggcag ggtatccatt
     7681 tacgtggcta tgcgcaaaaa aatcctaagc aagaatacaa acgagaagcc tttgaactat
     7741 ttggaagtat gcttgaagca atcaaagctg aagtaacaaa aatattgtta acggtccaaa
     7801 tcaagaatga gcagcaagtt gagactgcat ctgaagttca tacgcctgtt aacgtgcaat
     7861 atcagcatgc aacttatgaa gatgagctca ctcaggaaca acaagcgaca cctgaaaaag
     7921 catcacaaga aaacaaaaca aaaccctttg taagaaaaat ggataaggta ggtcgcaatg
     7981 acccttgccc ctgtggttca ggtaaaaagt ataaacaatg ccatggtaaa ttaaagtaac
     8041 ctgctaaccc cacgctggct ttaccagtat catgatacat tgcggtcttg aatggtaata
     8101 cgcgtcagaa gatcggttgt cgaaattcca tccgtatagt caatttcttt gatcatactc
     8161 gctggaagtt cggcacagag tttatatttt tcttgccgct ctttctcgga agagcaagca
     8221 aacgtataaa gatcatagcc ctgttgctgc ataaatgcag gcgttgcata gagcggccct
     8281 tcggtcacaa cagcatcaac atatttacag gctgaaacaa ctgcagctct ctcagactga
     8341 ctcattacag ggtgcttacc tttattttcc tgcacacgtt gatctgatac cacaaaaacg
     8401 gttaggtgcg tacccaacgc ttttgcttct cgcaagaaat taacgtgccc aacatgaaat
     8461 aaatcagcga ccatccgggt ataaactctt ttctgtgacc tattactcgg ccattcaggg
     8521 atagcttgat ttaatcgagt taacgcacgt tctagccact ggcgactgcg caaactgata
     8581 acgtcatctg ggtccttcaa tgcgatctgg tgcgcataac tccaagccct taccaattca
     8641 cccgataacc atcgctgaat caatcgatag taagcgaagc agcgtacgag caacgtgaat
     8701 ttttctagat ttactgctga aactaccgta tcccaataag gattgggggt tctccaattc
     8761 ccataaaatg cagttagtat cgtttcaggt ggattcggga accacacagt accataaggt
     8821 gtatctttgg gtattaaatc aaattttggg aaaatagaaa cccgctgata ttcatctgga
     8881 tagccgggaa gtgagaatcc tccaataatt cgatttgctt tatttaactg taagccgcat
     8941 agatcgagtg ttatgccaat ttcagaatga acataatccc gataattacg ataattaaaa
     9001 ctcatggggg ttctttgcca ccccattttc tcgagaagcg cacagcagtc atcccagttt
     9061 tccatccaaa cggcaatatc taaatcttta tcaaacgcta ataaatgacc atggcgaaca
     9121 atacccaaca aagtacccgc aaatggaaat gcacgaatac cgtttgcagc aagcgctgca
     9181 caagtttccc ataataatcg ctccgcccgg cctgataaaa aaatgttctc cgttgattcc
     9241 ttaactgttc tggatttctg atccgcaggt acttttcctg actttgttaa tgccattaaa
     9301 tgattaaggg cagtcatata gtgataacag gctgtttcca gctccaataa ctgaaaacaa
     9361 gcggtaccca tcatttgtgc ccaacgcgct ttttgtaaat caccctctgc aagactaaat
     9421 attttagccg cgtaaggcag tacttcacca gaacgccctg ctctcatcat cattgcacac
     9481 catagctgcg caacatccgt taactctgga taagtttgtt ttaagggttc gagaatctca
     9541 cttgccccat tcaaatcacc agaacggatc atttcctggg cctgccgcaa agattgttta
     9601 atcgcttcat tcataaaaat aatcaaattt gctgtctgat ccttgtattc taccgattat
     9661 ttgatactat taagaatcac tctcattatt atttatatta ttagagatag tgggataaaa
     9721 caaacttgtt taactttcag gagattacaa catggctatc actaaagaac ctcgccccaa
     9781 tctaagtaat tcaggcaata tatttgaaat catcgatgga atccagtttc ttggttttgg
     9841 ttcgggcggc ttcgatgcca atgatttact cattattggc gatgaactcg acaatttgct
     9901 agaaggtaat aatggcgctg atgatcacga tgagatcgaa ggaggtgctg ggaatgacag
     9961 attgcttgga cttaatggta atgacacgct tgatggtgat gaaggcaacg acgatctcaa
    10021 tggtggagaa ggaaccgaca aattattcgg tggattaggc gatgatatct taagggcagg
    10081 ttttggacat gaccgactca ccggcaacga tggtagtgat accttcggtt tttatgcggc
    10141 aggtttctat caagtaaaag atttttcgat tacccaaaat gatcgcttat tttttgattc
    10201 ccaaacgcta ggtatcaata gccttgaaca attagtcacc tatattacca atattgaaca
    10261 acgcgcagat gggtttaaag tttattttgg tgaagttgcc acaatcgaca ttgttggtgc
    10321 taatttatcg gctatcactg cagatatgat tgtcttcaat ctctaaataa acaccccgtg
    10381 gcaagaccac ggggaatcgc aagttgaata cagcgaagcg attacaaagt tttaattaaa
    10441 aacgctataa aaaatttgca ttgcctccat aactatgtat aatgagaatc attctcatta
    10501 atgattgtgg aggactgaaa atgtatcatt catacatgca cctacagact tcgaataaca
    10561 gcgatcattc ggcagcgaat gatcttcgta agtcaatcaa tagtgatgcg ttgttcacgg
    10621 gcaataatga aatcgtaatt caacatcgtg aacaacaata tcgcttgcgt ctaacgcgag
    10681 caggaaagct tattttgaca aagtaattta gccaaatcgt accaaggtca agacaaacag
    10741 ctaaattata atcacacaaa atcaaatgta attaggtttc tcatcggtcg ctggtaatct
    10801 cttctctcct catttaccag cgactttttt agccagccag cccaacccct catctgggca
    10861 agccagcctc ttttttgatg actttaggag cacggctatg aaattactta aattcccttt
    10921 atcgcagacc atttattgtg acaacagcca taattgggtt aattggcagc aaattagtca
    10981 catcactaat acaaagttaa aaaacattca aaattggatc actgttgtat ctgcaaagat
    11041 tttcagaagc cacgcattac gctcctcgac tccgctaaca acccgggtca ttccccttat
    11101 caaagagaaa caaatcaatc cccgcttgct gatcttcgcc aattataagc ctttcaataa
    11161 aacagaaggg ttggctggaa aaattattct gtgtatcggt ggtcgcattg cgctctattc
    11221 tgattatcaa aaattagtcg aaatagcggg tggaaatttc atcgggtatc ggggagctga
    11281 tatgcaatca gatcagctca agaaattgct tgatcaagcc aatatggtca tttgcccggt
    11341 tgactgtatt aaccatgaag atttcttttt tgtaaaaaat tactgcgcaa taactgggaa
    11401 aatttgcacc ttcttagcgc gatctaccct gcttaacttt caacaaggct taaaaatatt
    11461 gatcaatcta tctcatcaca acttaggaac tgtagtgtca tgaaaaataa aaaaattgat
    11521 gcagtaagtt tatttatagc attcctactg ggtgtgtttt ttattttatc aagccaactt
    11581 gaggcaaaaa caattcgaga atattggatt gctgcagaaa aagtaacctg gaactatgca
    11641 ccatcaggaa aaaatctaat tgaacctgag ggaaatctcg gcgtctgggg agaaacgctc
    11701 acctatacca agtaccaata cgtcggctac acagatggta gcttcaatgc accgattgct
    11761 caaccagaat gggccgggtt actcggaccc caaatccgcg ctgttgtcgg tgatactatt
    11821 aaagttcatt tccttaataa aacggatcgg cccttatcga ttcatcctca tggtatattt
    11881 tataaaaaag aggatgaagg cgcagatggt ggcaaaggta gcagtgtcca acccggtgag
    11941 cgctacacgt atacctggat cgccgatgaa gacgctgggc ctggtcctag tgatccgtcg
    12001 tccatcgtct ggttatacca ttcccatgta atggctgaag aagaaacaaa cctcggttta
    12061 atcggaacgc tgattattac gcgtaaaggg aaggaacgct cgcgtgatga tcctacccca
    12121 aaagatgtta ctcaagaatt tatcgcactt tataccatct ttgatgaaga aaatggtgaa
    12181 gagcctgggt taaaacatgc tattaacgga cgaatctttg gtaatctagc gggctatcaa
    12241 actcaactgg gtagtcgggt tcgctggtat cttgtagcat tgggtaatga agtagataat
    12301 cataccgtcc attggcatgg ccaaaccgta ttacatcgtg ggcaccgtac tgacgtcatc
    12361 gagctaatgc cagcaagtat gacaacggtc gatatggtgc ctcgttcagc tggaaactgg
    12421 ttgtttcatt gccatgtcaa tgatcatatg atggctggaa tgagtaccta ctggcatgta
    12481 aaatagtaac taaaatcgac aacatcacat tttcagcagc aaaaatagca ttctgttaag
    12541 attcgaatat tgatgcttga actttcgagc atcaatacta tcttagtatc actgcaatta
    12601 tccatcgggg gcgatccggt ttcgacgtgg gttgcaaagc agcgcagggc ataccgagga
    12661 tcag
//
LOCUS       DAOQKQ010000070        12404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_2385_length_12404_cov_5.246174, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000070 DAOQKQ010000000
VERSION     DAOQKQ010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 12404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12404
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2385_length_12404_cov_5.246174"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PKZ49_06140"
     CDS             <1..298
                     /locus_tag="PKZ49_06140"
                     /inference="COORDINATES: protein motif:HMM:NF037256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cadmium-containing carbonic anhydrase"
                     /protein_id="HND36177.1"
                     /translation="CQDHGANYEELVDAHNELGIAINMRQGTTIDRKAIRTTFGPQYD
                     LFVVDAWTFATAAQELALESNKQDADRIAKAITIQNVATASVLGHSSLPIIPIG"
     gene            501..1178
                     /locus_tag="PKZ49_06145"
     CDS             501..1178
                     /locus_tag="PKZ49_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HND36178.1"
                     /translation="MQILIIEDDFAIATNLYDYLESRGYGVDVAYDGIAGHCLAVTQH
                     FDVILLDLGLPKLDGLTICQKLRQESQIDTPILMLTARDTLDDKLKGFDHGADDYIVK
                     PFALKEVEARIVALHKRHSGKVTSRTLVVGDLLFDPKTMSITFAGSAVKLPPKCIRLL
                     ALMMSEPGRVFGRKELESVVWGNTQETSDTLRTHMHVLRRSLSQAGGYDPIETVHGLG
                     YHLISRA"
     gene            1171..2457
                     /locus_tag="PKZ49_06150"
     CDS             1171..2457
                     /locus_tag="PKZ49_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HND36179.1"
                     /translation="MRKHRLRHGLRQRVALALAIFCIFFVSIFGISLYVVSGDIEEDH
                     IEQILRMEMEHLIYRYEQHHDIRPQIGSNLEGYIITSIADEKKLPSYLQGLNFDYHTI
                     FLRQEEIRVAVKHINEIKFLVAYRIGLHEVRQRELRLILVISLVIVITVVFVVGHMLA
                     GLVVKQVTQLADRVRRLAPGNINGEKLSQPGQDDEIALLARLIDDYQVRITRMLRREQ
                     EFTANLSHELRTPITTIQTSCELLLTEPNLPERTYRRIKMIEGASARMGEHIQALLFL
                     AREQDLGEIELVNITECILEIVDSLQNEISQKQLRFQINVPNNATLKLNRQALNIVLI
                     NLIRNAVQYTEEGFITVEFRDRQLSIIDSGIGIEPASMPFLYDRFFRATTQGRGLGIG
                     LAIVKRICDYYEWRIEVDSKPGRGTTFKLTFPLSTF"
     gene            2555..4672
                     /locus_tag="PKZ49_06155"
     CDS             2555..4672
                     /locus_tag="PKZ49_06155"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HND36180.1"
                     /translation="MTITKDDRKISIIGGLLLLGLTLTTGFTVYNAMRQQIETVLDRG
                     LTGSLDGKALLFETRIGKGIEDARALALRPFIIQFMQELNENINNREASNNLDRNVNS
                     LTQIGFSAAVIYDKNDNILSQVGTFSSDENPVITLTSERNAKLIWDNVFVLRVAVDVI
                     NNNNRIGTIVTETKLPNLTRSFMDIQSGGKSVEFLICAKMPEKQLVLTCLIRNPTSVR
                     YAYLDISSKEQNATPKGMALIGKSGVASTLDYRGIPVIEAYTSLPSLGLLMTLKLDQE
                     ELFMPINDKLKDSALYLIILIIAEIILLNWFIGKLITSEKNAQSSKRKAEEYSDELTL
                     KETVLRKKLNEITCLYEIRREMEFEISIDEVCHKILKLLPPALQFPKTSTVEIIFNNK
                     RFTSKDLDEESKTEIGQTPSYIEILETPKIANRKSIFNIYIQSNIYIDIENQGHLRVY
                     YIENVPSSLTEEQIFIDAIARDLEKWIALRKLESTLISVADDQVHKIGQELHDNLGQQ
                     IAAVSFQASALELQIRENKTPHETILELAASIAAQTQGMVTSIKQVSKILLPFELEAN
                     GLITAFYSLTTQISETYNINCVFVTHLKNDRNIDSVVGLNLYRVAQEAINNAIFHGKA
                     KNISISLSITNNELKTKFIHLTISNDGKGINEETIYSSAGMGIKIMQYRAKQLNGKLT
                     ILKHKDGGIEINFIVPILESDKS"
     gene            4683..5315
                     /locus_tag="PKZ49_06160"
     CDS             4683..5315
                     /locus_tag="PKZ49_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HND36181.1"
                     /translation="MLVDDHAMIRHGLTLLINMENDLEVLCEADDGPEAIKVLKKNFP
                     IDLVLLDITLKTVSGLELMSSILSLYPSLPVLFISMHDESVYAERALRAGARGFVMKQ
                     EPRTILINAIRQVLKGNIFISKNIQDQVLKRIVIRGYGAEATINALTPSEFEILHLIS
                     LGYNTHEISNLLSRSTKTIETHRYNIRTKLKLRDTADLIRYATKWFTDKK"
     gene            complement(5504..5734)
                     /locus_tag="PKZ49_06165"
     CDS             complement(5504..5734)
                     /locus_tag="PKZ49_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36182.1"
                     /translation="MAINKTLLITLMTILATLLGLYMGSISNNGIMIEANENSEVTDC
                     LWIGGALFINYLLLVCYFAPETSPLLKSNSRI"
     gene            complement(5787..6194)
                     /locus_tag="PKZ49_06170"
     CDS             complement(5787..6194)
                     /locus_tag="PKZ49_06170"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HND36183.1"
                     /translation="MFKRIYVAIDQSEFSKQALIAATKIAQTFKASLCLGHCITDDTQ
                     SNQESALKFLEDTKSQITDIGDIEVKISVADGQYGLNGISTAIAQSANDWQSDLLVVG
                     TSNRKGLDRFFLGSVAEELVSKINCSVMLFRIN"
     gene            complement(6274..7623)
                     /locus_tag="PKZ49_06175"
     CDS             complement(6274..7623)
                     /locus_tag="PKZ49_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HND36184.1"
                     /translation="MATNISSDSGYESNDKSQKSQSLTSQYGSAAFQSQYFFRDTAAG
                     LITGAMAIPLSIGIAIMSDYPIKVGLATVAFASLIGWLFAWFRPGNYIGSPGIAAGLA
                     PVLALGVASFGWENMAFCIFMTAVMQAIIWKFNWQRYLLVAVPVYLVEGLLAGVGLKI
                     FLKFFEFTYEIPAEMVTEVFWNEARIQMVAISAAGVATFLYLFAKFKDKQPAIPYFVL
                     IIGGVILAQFVSVPMIHVEDVDLHFKLPLPADDIAISMLVYMILFCAMLAIVDVIEQV
                     MSNAAIEKIDPLKRKTDSNNSLLAIWVANLGSSFFHGMTNLDGLAKSTTNKLAGAMTK
                     FSVLIIGSVVCFFLFNVHYLDFLPKFALAAIMLFTGYKMVAGLVHVTHYGSYALMLAI
                     ITAGLVFKVGIFEGLLAAMVIHGIIHYMVYTNHNQMPGKEVIRRYFRSLKSDNSNLQ"
     gene            complement(8032..8244)
                     /locus_tag="PKZ49_06180"
     CDS             complement(8032..8244)
                     /locus_tag="PKZ49_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36185.1"
                     /translation="MSRKVIIGLTAILFLISLSVIPMVLADELSFNWLIVDCVIILTT
                     YVALIYPEVFNAVKRSTKQFKMRYFA"
     gene            8815..9765
                     /locus_tag="PKZ49_06185"
     CDS             8815..9765
                     /locus_tag="PKZ49_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent bicarbonate transport family
                     permease"
                     /protein_id="HND36186.1"
                     /translation="MGPTIQPVVLFFVMGVIAGVIKSDLKIPEALYKSLSIFLLIAIG
                     LKGGVSLSKYDIMEVLPTSLTLVFSAALIPLAAYPILRFLGKFNQADSGAIAAHYGSV
                     SAVTFAVGLAFMDGIKEYYEGYMPLFLALLEVPALVTGTVLALMGAGGKKTEWGKLMH
                     EVLTGASIYLLMGAICIGYYAGSKNIAPLDKLYMDLFIGALSLFLLEMGLLAASRIGD
                     VIRKGPFMLVFGICFPLAAGLLGAFLGWAFGLSPGGTMVLAVLYASASYIAAPAAMRI
                     AVPAADPAMSIGLSLGITFPFNIFIGVPIYWEMAKWFHGV"
     gene            9915..10232
                     /locus_tag="PKZ49_06190"
     CDS             9915..10232
                     /locus_tag="PKZ49_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HND36187.1"
                     /translation="MNGTIAMKRIEIVVTEEELENLIKLLNSAGVRGYSYLKEVGGLG
                     SRGVRNPNDVIWEEENAIVILACKEDQAQKIVDILRPKLKEFGGMCLVSDCLWIEGPA
                     VSY"
     gene            complement(10326..11387)
                     /gene="hemE"
                     /locus_tag="PKZ49_06195"
     CDS             complement(10326..11387)
                     /gene="hemE"
                     /locus_tag="PKZ49_06195"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HND36188.1"
                     /translation="MTKLINDTLIRALLRQPTEYTPVWLMRQAGRYLPEYNQTRARAG
                     DFLSLCKNPNFATEVTLQPLARFPLDAAILFSDILTIPDAMGLGLYFSEGEGPRFERP
                     LREEWEIRALTVPDPEVHLRYVIDAVSQIRKALNGQVPLIGFSGSPFTLACYMIEGGG
                     GTDFRQIKTMLYQRPDLLHHILSVNAQAVTDYLNAQIEAGAQAVMIFDTWGGALSHEA
                     YQAFSLQYMKQIISQLKREKDGVRVPSIIFTKGGGLWLEQMVDSRCDAIGLDWTIQLA
                     DARKRVQDKVALQGNIDPAVLFATPEVIKHEVAKVLASYGKGSGHIFNLGHGISQFTP
                     PENAFALVEAVHSLSRQYH"
     gene            11779..12210
                     /gene="rpsF"
                     /locus_tag="PKZ49_06200"
     CDS             11779..12210
                     /gene="rpsF"
                     /locus_tag="PKZ49_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HND36189.1"
                     /translation="MRHYEIVLIIHPDQSEQVPAMIERYSSMITSKSGKIHRVEDWGR
                     RQLCYIIQKVHKAHYIIMNIECDQETLSDLEHTFKFNDAVLRHLTIRMKAAVTTPSVM
                     MHEGKAKSTLIEDEEDSVSEETGETSEVSDQKSPDQAVNQP"
     gene            12224..>12404
                     /locus_tag="PKZ49_06205"
     CDS             12224..>12404
                     /locus_tag="PKZ49_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HND36190.1"
                     /translation="MNCNLTTICGKLIKLNQIRYTPAGIEVIEFEINHNSTQTEASIH
                     RQITCVITAIAMAHLA"
BASE COUNT         4000 a         2395 c         2314 g         3695 t
ORIGIN      
        1 ctgtcaggat catggtgcga attatgagga actcgttgat gctcataatg agctgggtat
       61 cgcaatcaat atgcgacaag gtactacgat agatcgtaaa gcgattagaa caactttcgg
      121 acctcaatac gatctttttg tggttgatgc gtggactttt gcaactgccg cccaggaatt
      181 agcactagaa agtaataaac aagatgctga ccgaatcgcc aaagccatca ccatacaaaa
      241 tgtagcgacc gcatctgttc ttgggcactc atctctaccc atcataccaa ttggctaatt
      301 attggattat tggctcaaac ctcaatcttt tgctattgta ttcataaata ggtgccaaag
      361 aggctagttg tgaattttct agcctcttgc atctgaaatc attctcctaa agtagcacac
      421 acaatataat caatggacaa ttgagttttt aataagactt aatgtctaat gaaaataatg
      481 gatccagttg gaaattagaa atgcagattt tgattattga agatgatttt gcgattgcaa
      541 cgaacctgta tgattatctt gaatctcgag gatatggggt tgatgttgcc tatgacggaa
      601 ttgcgggaca ttgcttagct gtcacccaac attttgatgt aatcttgcta gatctgggat
      661 tacccaaact agatggatta acgatctgtc aaaaacttcg ccaggaatcg caaatcgata
      721 cccccatttt aatgttgact gcgcgcgata cccttgatga taaattgaaa ggcttcgatc
      781 atggtgctga tgattacatc gttaaaccct ttgcgctcaa agaagtagaa gcgcggattg
      841 ttgcacttca taaacgccat agtggcaaag taacgagtcg tacgctggtc gtcggggatt
      901 tattatttga ccctaaaacc atgtccatta cttttgctgg ctcagctgtc aaattaccgc
      961 caaagtgtat ccgtttgctt gcgctcatga tgagtgaacc tggccgagtg tttggacgca
     1021 aagagcttga gtccgtagta tggggcaata ctcaagaaac cagtgatacc ctgcgcactc
     1081 atatgcatgt attaagacga tcattatctc aagcaggtgg ctatgatccc atcgaaacag
     1141 tgcatggact gggttatcac ttaatatctc gtgcgtaaac atcgattaag gcatggtcta
     1201 cggcaacggg tcgcactagc gcttgcaata ttttgtatct tttttgtcag tatctttgga
     1261 attagtcttt atgtcgtttc aggcgatatc gaagaagatc atatcgagca aattctcaga
     1321 atggaaatgg agcatctcat ttaccgctat gagcaacatc atgacataag gccacaaatc
     1381 ggctctaatc ttgaaggtta tatcattact tcgatcgccg atgaaaagaa acttcctagc
     1441 tatttacaag gacttaattt cgactatcac accatatttc tccggcaaga agaaatccgg
     1501 gtagcagtca aacacatcaa cgaaatcaaa tttctagttg cttatcgaat cggtctacat
     1561 gaagttcgcc agcgtgaatt acggctaatc ctcgttatat ccttggttat tgtgatcaca
     1621 gtcgtttttg ttgtggggca catgcttgca ggtcttgttg tgaaacaagt cactcaatta
     1681 gcagatcgtg ttaggcgttt agcgcctggt aatataaacg gcgagaaact ctcacaacct
     1741 gggcaagatg atgagattgc attacttgct cgcctaatcg atgattatca ggttcgaatt
     1801 acccgtatgc tgaggcgcga gcaagaattt acagccaact taagccatga actccgaaca
     1861 cccatcacaa ccattcaaac cagttgcgag ctattactca ccgaaccaaa tttacctgaa
     1921 cgaacttacc gacgtatcaa aatgattgag ggtgcttctg ctcgcatggg tgagcatatt
     1981 caagccttac tctttttagc aagagaacaa gatctaggtg aaattgaatt agtcaatatc
     2041 actgaatgta tattagagat tgtcgactca ttacaaaacg aaatttctca gaaacagctt
     2101 cgcttccaaa tcaatgttcc taacaatgca acactcaagc ttaatcgaca agccctaaat
     2161 atcgtattaa taaacttaat tcgcaatgca gtacaataca cagaagaagg attcattaca
     2221 gtagaattta gagatcgcca attatccatc attgattcag gaattggtat tgaacccgct
     2281 tctatgcctt ttctatatga tcgcttcttc cgggcaacaa ctcaaggacg aggtctgggt
     2341 attgggcttg cgattgtcaa gcgtatttgt gactactacg agtggcgtat tgaagtggat
     2401 agtaaaccag ggagaggaac gacatttaaa ctcacctttc ccttgtctac cttctagata
     2461 atgtatttca gaaagccccc ctttattcct aagctaaccc ctcgagatgt gaaaatgatt
     2521 tgttttgttt ttgaccgtca ttgtaggaaa tgtcatgact atcacaaaag atgatcgcaa
     2581 aataagtatt attggcggcc tacttttatt agggctaaca ttaacgacgg gcttcactgt
     2641 ttacaatgca atgcgacaac aaattgaaac tgtattagac agaggtctca ccggatcgtt
     2701 agatggaaaa gctttactgt tcgaaacaag gattggaaaa ggaatagagg atgctcgtgc
     2761 attagcatta cgacccttta ttatccaatt catgcaggaa ctcaatgaaa atatcaataa
     2821 ccgagaggca agcaataatc ttgaccgtaa cgttaattcg cttactcaga tcgggtttag
     2881 cgctgcggtt atctacgata aaaacgataa catcttatcg caggttggta cttttagctc
     2941 ggatgagaat ccagtgatta cactaacttc tgaaagaaat gctaaattaa tatgggataa
     3001 cgtctttgtt ctaagggtag cagttgatgt tattaataac aataacagaa ttggtacgat
     3061 tgtcacagaa acaaagcttc caaatctaac tcgaagtttt atggatatcc aatctggcgg
     3121 aaaaagtgtt gaatttttaa tctgtgcaaa aatgcctgaa aaacaactag tgcttacctg
     3181 ccttattagg aaccctactt ctgttcgata cgcatatcta gatattagct ctaaagaaca
     3241 aaacgctact cctaaaggaa tggcgcttat cggtaaaagt ggggtcgctt ctaccttaga
     3301 ttaccgtggt attcctgtca ttgaggccta cacttcatta ccgagtctag ggctcttaat
     3361 gacattaaag ttggatcaag aagagttgtt catgccaatc aatgacaaac taaaagatag
     3421 cgccctttac ttaatcattc taattatcgc tgaaattatc ttactaaatt ggttcatcgg
     3481 aaaacttata acatccgaga aaaatgctca gagctcaaaa cgtaaggctg aagaatactc
     3541 cgatgagtta actttaaaag aaactgtttt acgcaagaaa ctaaacgaaa ttacctgcct
     3601 ttatgaaata cgtcgggaaa tggaatttga aatctcaatc gacgaagttt gccataaaat
     3661 ccttaagctt ttaccgcctg ctttacaatt tcctaaaaca agtactgtag aaatcatttt
     3721 taataataaa cgttttactt caaaagatct ggatgaagag tctaaaactg agataggaca
     3781 aactccctcc tatattgaaa tactagaaac acctaaaata gcaaatagaa aatctatctt
     3841 taatatttat attcagtcca atatttatat tgatattgaa aatcaaggac atttacgcgt
     3901 ttattatata gaaaatgttc cttcttcctt gactgaagaa caaatattta ttgatgctat
     3961 tgcaagggac ttagaaaaat ggattgcgct acggaaatta gaaagtacct taatatcagt
     4021 cgcagatgat caagttcata aaattggtca agagcttcat gataatttag gccagcaaat
     4081 tgctgcagtt tcttttcaag cgagtgctct ggaattgcaa attcgtgaaa ataaaacacc
     4141 ccatgaaaca attctagaac tagcagcttc tatagctgct cagacccagg gcatggtaac
     4201 aagtattaag caagtatcaa aaatcttatt accttttgaa ttagaagcaa atggtttaat
     4261 tactgctttt tactcgctca ccactcaaat tagtgaaact tataatatta attgcgtgtt
     4321 cgttactcac ttaaaaaatg atcgtaatat cgatagtgtc gttggtttaa atctttatag
     4381 agttgctcaa gaagcaatta ataatgctat ttttcatggt aaagcaaaaa acatatctat
     4441 ttccctatca ataaccaaca atgaattaaa gactaaattt atacacctta ctatttcgaa
     4501 tgatggtaaa ggcattaatg aggaaacaat atattcttct gcaggtatgg gtataaaaat
     4561 catgcaatac cgtgcaaagc aattgaatgg caaattaaca attcttaagc acaaagacgg
     4621 tggaatcgaa attaatttta tagtaccaat tttagaaagt gacaaatcat aaatctaaaa
     4681 tcatgttggt tgatgatcat gctatgataa ggcacgggct gactttgctg atcaacatgg
     4741 aaaatgattt agaggtactt tgtgaagcag atgatggacc agaagctatc aaagtactaa
     4801 agaaaaattt cccgatcgat ttagtactgt tagatattac gctcaagaca gtatctggcc
     4861 ttgagttaat gagtagcatt ctatcactat acccttcttt gccagtttta ttcatttcaa
     4921 tgcacgatga atcagtttat gcagaacgtg cattaagagc tggcgcacgc ggctttgtta
     4981 tgaagcaaga acccaggact atattaatca atgcaattcg acaagtacta aaaggtaata
     5041 tctttataag caaaaatata caagatcaag ttttgaaacg aattgttatt cgaggttatg
     5101 gagctgaagc tacgattaat gctctgacac ctagtgagtt tgaaatactg cacttaatta
     5161 gtctaggata caacacacat gaaatatcaa atctattatc acgtagtact aaaacgattg
     5221 aaacccatcg gtacaatatt cgcacaaaat taaaattaag agataccgcc gatttaattc
     5281 gttatgcaac caaatggttt acagacaaaa aataaaaaaa gaccctaact agcagggact
     5341 agttagggag aggaaatggc gcttagagca ccgggagcaa cattcaacta atcttcctat
     5401 cacgaaaaat ataattgatt aaacctagca agtaatgtcg acgacttgct tgcctatgtg
     5461 atttaaccaa ttatctatct tgatattagc aaccagcaga acgttaaata cgcgagttac
     5521 tttttaataa gggagatgtt tctggtgcga aataacaaac caacaataag taattaataa
     5581 ataaagcacc acctatccac aaacaatcag ttacttcgga gttttcatta gcttcaatca
     5641 tgattccatt atttgaaatt gaacccatat acaatcctag taaggttgcc agaattgtca
     5701 ttaaagttat taataaggtt ttattgattg ccattgctat atttccttta gattaaaaat
     5761 attttgatta cgtcacagca aactgactaa ttgattctga acagcattac tgaacagttg
     5821 attttgctga ctaactcttc tgcaacagaa cccagaaaaa agcgatctaa cccttttcga
     5881 tttgaagtac caaccactaa taaatctgat tgccaatcat ttgcagattg agcaatagca
     5941 gtactgattc cattcaagcc atattgccca tcagcaacag aaatttttac ttcaatatcc
     6001 cctatatcag taatttgtga tttagtgtct tctaaaaatt ttagggctga ttcttgatta
     6061 gattgcgtgt catcagtgat acagtgacct aaacataacg atgccttaaa agtttgagca
     6121 attttcgtag cggctattaa tgcttgcttg gaaaattcac tttgatcaat cgcaacatag
     6181 attcttttga acatagttat ttatttcttt gataattaat taaatttctc atagcagcgc
     6241 aaataatctg cgctgctaat aaagactagt tatttactgc aaatttgagt tatcgctctt
     6301 aaggctacgg aaatatcttc taatgacttc tttgccaggc atttggttgt gattggtata
     6361 aaccatataa tgaataattc catgaatcac cattgctgct agaaggccct caaaaatacc
     6421 cactttaaat actaagcccg cagttataat tgctaacata agtgcatatg aaccataatg
     6481 cgtaacatga actagcccag ccaccatctt atagcctgta aacaacatga tggcagccaa
     6541 tgcaaattta ggaagaaaat caagataatg cacattgaag aggaaaaaac aaactactga
     6601 accgattatc agcactgaga atttggtcat agcaccagct aatttattgg ttgtactttt
     6661 agccaaacca tccaagttgg tcataccatg gaagaagctt gagccaagat tagctaccca
     6721 aattgctaac aaactattat tactatctgt tttgcgtttt agtggatcaa tcttctcaat
     6781 cgctgcatta ctcatcactt gctcgatgac atcaacaatt gccagcattg cacaaaacaa
     6841 aatcatgtaa accaacattg atatcgctat atcatcggct ggtaagggta atttaaaatg
     6901 taagtcaaca tcttcaacat gaatcattgg aacactaacg aattgtgcca gtatcacgcc
     6961 accaataata agtacgaaat aaggaatcgc aggttgcttg tctttgaatt tagcaaaaag
     7021 ataaagaaag gttgctactc ctgctgcgga tattgctacc atttgaatgc gtgcttcatt
     7081 ccaaaaaact tcagttacca tttcagctgg aatttcataa gtaaattcaa agaatttcaa
     7141 gaaaattttt aatccaaccc cagctaataa accctccact aaatataccg gaacagcaac
     7201 aagcaaatat cgttgccaat taaatttcca tataatggct tgcattaccg cagtcataaa
     7261 aatgcagaaa gccatattct cccagccgaa cgatgctacc ccaagtgcta gcactggcgc
     7321 caaaccagct gcaatacctg gtgaaccgat gtaattgccc ggcctgaacc aagcaaatag
     7381 ccaaccaatt agtgatgcaa atgctactgt cgccaaacca actttaatcg gatagtccga
     7441 cataatggcg attcctattg ataatggaat tgccatcgct ccggttatta atccggcggc
     7501 cgtatcacga aaaaagtatt gagactgaaa agcagcagat ccatattgac tcgttaaact
     7561 ttgtgacttt tgtgacttgt cattactttc atagccactg tcactgctta tattcgttgc
     7621 catagactaa ttccttgttt taaaaaatat taaaaccaat tcattcaaga atttagaagc
     7681 aaatccttaa atccaaacta cgggaatagc ctacttctac gctggatact ttgtatattg
     7741 agcagagtat taattttcat aatgaaaaaa attagagaaa ctaatacttt tacttagatt
     7801 tattgagtat tttacttgaa cgttattaac gtcttttgat gagaaaatta taaatatcag
     7861 aaagttacat cagaaattca ttcctaggtt gtattactag acaattcggt aggaaattca
     7921 ccgaattgtc tatctaatct gcttaaccat ttgactataa agaatattta ctactgggaa
     7981 tgaccagagg taaaattatc ttaagccaaa taagctgagt agttacgtcg attatgcaaa
     8041 atagcgcatt ttaaattgct tggtcgaacg cttaaccgca ttaaatactt caggatagat
     8101 taacgcaaca taagtcgtca gtatgatgac gcaatcaaca atcaaccaat taaagcttaa
     8161 ttcatcagct aacaccattg gaattacact taatgaaatt aaaaacaaaa ttgccgtcaa
     8221 accgattatt actttcctag acatagctaa taactccttt aatttttaaa caatctaatt
     8281 atacaccaat aagatcaaac aaatcacaaa aagttcacaa tagatttatg tttaatttac
     8341 acaaaactaa caaaattatc atcacataaa aactttgaga ataaataacg ataaaagcca
     8401 gtccatctgg ctatttcaag aaatagtgga agtcattcca acagcaatag tcgcctaaac
     8461 tgagataact aactatagcg aagcatttca tataaattcg cttagagatt aaattgataa
     8521 gcatgaatta tttaggactt ctcataaact ttattaattc ctaacatttt cattacactc
     8581 ccttaacgta ctttgtgcta atttccatat ggaggcattt ttttgttctc gagcttctgt
     8641 cgccaacgat ctaagggaaa atatcattct cgattaatta tcaaaattat cagtcagtaa
     8701 acgatatttg atagccaagc gttagagggt atggaaagtt aggttattgg taagtaaaat
     8761 aaatttattt tgaaactatt taatataatt acaaattaaa accgggagta acacatggga
     8821 ccaacgatac aaccagtagt attgtttttt gttatgggag taatagctgg ggtcattaag
     8881 tccgacctca aaatcccaga ggctttatat aaaagtttaa gtattttttt attgattgcg
     8941 atcggactta aagggggcgt atcactttct aagtacgata ttatggaagt gctgccaaca
     9001 tcattgacgt tggtattctc agctgcgctg attcctctcg cggcttatcc aatattaaga
     9061 ttcctaggaa aattcaatca ggccgattct ggggcaattg cagcacatta tggttcagtt
     9121 agtgctgtaa cctttgctgt aggtctcgcc ttcatggatg gtattaagga atattatgaa
     9181 gggtatatgc ccctcttctt agcgttgctt gaagtgcctg ctcttgtcac aggtactgtt
     9241 ctagcgctga tgggtgcagg tggcaagaag accgaatggg gtaaattaat gcatgaagta
     9301 ttgaccggcg caagtattta cctgctcatg ggtgcgatct gtattgggta ctatgcagga
     9361 agtaagaata ttgcaccact tgataagctt tacatggatc tcttcatcgg tgcgttatca
     9421 ttattcttat tagaaatggg tttgttagca gcatctcgta ttggtgatgt gattaggaaa
     9481 ggccctttca tgcttgtttt tgggatttgc ttcccgctag ctgctggttt attgggtgca
     9541 ttcttaggat gggcattcgg actatctcct ggaggcacca tggtattagc agtattatat
     9601 gcgagcgctt cttacattgc agcacctgct gccatgagga ttgctgtgcc tgcggccgat
     9661 cctgccatgt cgattgggct ctcattgggc attactttcc cattcaacat cttcattggc
     9721 gtacccattt attgggaaat ggcaaaatgg ttccatggag tataagaaca gcttgtcact
     9781 acgattagat taaacgactt atcttgatgt aattatctga attgtaatta gatagggttc
     9841 tacgctaggg taagtcagaa tcaatcaata ctcttaaaat aggccttttt aaaataactt
     9901 aataggagaa agtcatgaat ggcactatcg caatgaagcg tatcgagatt gtcgtcacgg
     9961 aagaagagtt agagaactta atcaagttac tcaatagcgc aggtgttcgt ggttattcgt
    10021 atctaaaaga agtaggggga ttaggttctc gtggcgttcg taatccaaat gatgttatct
    10081 gggaagaaga aaatgcaatt gtgatcttgg cttgcaaaga ggatcaagcg cagaaaatcg
    10141 ttgacatcct tcgccctaaa ctcaaagaat ttggtgggat gtgtttggtt tctgattgtt
    10201 tgtggatcga aggaccagcc gtttcttact aaacttcctc aaattacgta tccatcttgc
    10261 ctcaaaagtg agatggatac gcattacact cctcgaatat ttttgtacgc tattaattat
    10321 aatcgttagt gatattgtcg gctgagagaa tgaaccgcct ctaccaaggc aaatgcgttc
    10381 tctggtggtg taaactggga aataccatga cctaggttaa atatatgccc cgatcctttt
    10441 ccataactcg ccaatacttt tgcaacttca tgtttgatga cttccggtgt ggcaaataaa
    10501 acagctggat ctatattacc ttgcaaagcg actttgtctt gtacacgctt tctcgcatct
    10561 gctaattgaa ttgtccaatc caagccgatt gcatcacatc gactatcaac catttgttct
    10621 agccataatc cccctccctt agtaaagatg atactaggta ctctcacccc gtctttctcg
    10681 cgcttcaatt gggaaatgat ctgtttcata tactgtaaag aaaatgcctg ataagcttca
    10741 tgcgatagcg ccccgcccca tgtatcaaag atcatgactg cttgggcacc cgcttcgatt
    10801 tgagcattca agtaatcggt caccgcttgc gcattaacac tcaatatatg atggagcaga
    10861 tcggggcgtt gatatagcat agttttaatt tggcgaaaat cagtgccacc acccccttca
    10921 atcatatagc aagcgagtgt gaaagggctg cccgaaaagc cgatgagagg tacctgtcca
    10981 tttaatgctt tacgtatttg tgaaacagca tcaatgacgt agcgcaaatg tacttctggg
    11041 tctggtaccg taagcgcacg aatttcccac tcctcacgca gcggacgctc aaaacgcggc
    11101 ccttcacctt cggagaaata tagtcctagt cccattgcat ctggtatcgt cagaatatcc
    11161 gaaaataaaa ttgctgcatc tagcggaaat cgggccaatg gctgcagggt cacttcggtc
    11221 gcaaaatttg gatttttgca tagtgaaaga aaatcgccgg ctcgtgcccg cgtttgatta
    11281 tactctggta aataacgccc agcctggcgc ataagccaaa caggggtata ttcagtgggt
    11341 tgacgaagta aggcgcgtat tagcgtatcg tttataagtt ttgtcattaa tcaatctatc
    11401 tttataaata aaatcaaaat catgatgcat tatttaaatt aataatgctt tgttatttat
    11461 tatttgtgtg atccctctac attgcaatat taatgtacca agggttaaca tggatccgcg
    11521 aaaatttaag ttcctcgatc gcaaatactt agtaccatca tcatcacatc aaaactatgt
    11581 gtgctactgc acatatttta tgctttcgca ctcaagattt atgaaatatt ttttcgctat
    11641 tttactgagt taacaaaagt tattttgatc ttcactgatt gattcagtaa aatagagtgc
    11701 ttatttttga ttttaaccct tgcctcacca actatgaata agattattcg cttgggaggc
    11761 tttacccaag aggaattcat gagacattac gaaatagtat tgattattca cccggatcaa
    11821 agcgagcaag tgccagcgat gattgagcgt tatagtagta tgatcacgtc taaaagtggc
    11881 aaaatccatc gtgtagaaga ttggggaaga cgccaattgt gctacatcat ccagaaagta
    11941 cataaagcac actacattat aatgaatatt gagtgcgacc aagaaacact cagtgatttg
    12001 gagcatacat ttaaatttaa cgatgctgta ctacgccatc taacgattcg catgaaagca
    12061 gcagttacca caccttctgt aatgatgcat gaaggtaagg ctaagtccac actcattgaa
    12121 gatgaagaag atagcgtttc tgaagaaacc ggagagacta gcgaagtcag tgatcagaaa
    12181 tcacccgatc aagccgtaaa tcagccctaa tgcaggcgaa cgcttgaatt gtaatctgac
    12241 gacgatttgt ggaaaactga tcaaactgaa tcaaatacgt tatacaccgg ctggaattga
    12301 ggttattgaa tttgaaatta atcataattc aacgcagact gaagcaagca tccaccgaca
    12361 gattacttgt gttatcacag cgatagcaat ggcacatctt gcta
//
LOCUS       DAOQKQ010000071        12252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_2419_length_12252_cov_5.661064, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000071 DAOQKQ010000000
VERSION     DAOQKQ010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 12252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12252
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2419_length_12252_cov_5.661064"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="PKZ49_06210"
     CDS             complement(<1..658)
                     /locus_tag="PKZ49_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HND36191.1"
                     /translation="MITAFQAKYFAHELTRRFPTDSAEKLAGAVASAQVDINLHQVDA
                     ALFAFNSPLSKGALLADEVGLGKTIEAGLVLSQKWAERKRRILIITPSNLCKQWHQEL
                     NEKFFLPRYILEAKSYNAAIKQGQFRPFESPEIIICSYQFAKSKAADIHAIPWDLVVI
                     DEAHRLRNVYKPSNVIANTLKMALAGKHKLLLTATPLQNSLLELYGLVSFIDEHTFGD
                     L"
     gene            complement(1013..3568)
                     /gene="mgtA"
                     /locus_tag="PKZ49_06215"
     CDS             complement(1013..3568)
                     /gene="mgtA"
                     /locus_tag="PKZ49_06215"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HND36192.1"
                     /translation="MEKNPEIAVYWSQPADSMMKSLGSNSQGLDPAIAADRLMHTGAN
                     RLKTNHFSGSTLQLFLNQFRSPLVLILIFAAIIALLVKDWLDALIVLMIVGMTAVLSF
                     IQEHRATRAVEKLRQKIATRSTVLRGGQTLVVPSDTIVPGDIVVLSAGNLIPADGMLL
                     EAKDFHVAQALLTGETFPVEKKPGISAPEASLVDRNNCIFMGTSVRSGYAKALIIQTG
                     LTTAYGKIAETLSLRPPETEFERGLRHFGQLLVRIMVIMVIAILAINIVLQHPTIETV
                     LFALALAVGLSPELLPAILTITLARGAKAMAAQGVIVKRLNAIENLGSMDVLCTDKTG
                     TLTKGVVQLGQALDSLGQTSKLVLQLAYVNSRLQTGIINLLDEAIIVAGEQSNITLTG
                     YQKLDEIPYDFVRKRLTTVAKHEMDATATMICKGAFQSIIEICDQIQLASHIEPLTSE
                     HQQQLLQHYTQWSMQGYRVLAVAQKSVAINNNYTRDDEHSFVWIGFLLFFDPPEAGIT
                     DTLKALTQLGVQLKIITGDNRYVACHVAEAVGIDTQTIVTGQALADMKDEALWHLAPQ
                     AGVFAEVDPNQKERIITALQKTGHVVGYLGDGINDAPALHAADVGISVENAADVAREA
                     ADFVLLKHDLNVLCQGIDEGRHTFANTLKYIFITTSANFGNMISMALASLFLPFLPLL
                     AKQILLNNFLSDIPAMGIAGDRVDRNWERTPHRWDVTLIRNFMITFGLVSVVFDLLTF
                     GVLLYLAGEVKAIFRTGWFVESLLTELLIIFVIRTYKPFYRGQPGRFLIWSVIGIALI
                     ALLLPYVPIGSLFSFVPLPLPVMIAIMIITLLYVITSEVVKQLFFKRLNRKSL"
     gene            complement(3690..5402)
                     /locus_tag="PKZ49_06220"
     CDS             complement(3690..5402)
                     /locus_tag="PKZ49_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HND36193.1"
                     /translation="MPHSTILITTIAVSLGLALIMGMLANRLKLPVLVGYLVAGMMIG
                     PFTPGFVADLALSNQLAEIGVILLMFGVGLHFSLSDLLAVRKIALPGAIAQIIVATLL
                     GASVAMTWEWNLGASIVFGLALSVASTVVLIRALEQRGLMKSINGRIAVGWLIVEDLV
                     VVLALVLLPPLAGWLGGQGDDSSTSNNITDLLIVLAITFVKVSIFIGFMLIVGKRLFP
                     WLLWHVASTKSNELFILCVIAVAVGIAYGAAKLFDVSFALGAFFSGMVMRESSLSHRA
                     AQESLPLRDAFSVLFFVSVGMLFDPNILIEEPLHVLIVVAIILFGKSLAAFILVLAFR
                     YPLNTALTVSVSLSQIGELSFILASLGVSLELLPLEGYSLILAGAFISITLNHLVFET
                     VEPIQNWIRSRSKLARRLERTDDPLAELPTSVASTHVTGHVVIVGYGAVGKRVSETLA
                     SQGTHYVVVDQNREIVEQLRKHHAHAVAGDAADPAVLIQAHIARAGAMIITAADVLRI
                     QQMVETAKILNPQAEILISTHDPEAAARLEKENMGLVFLDEQVLAQNITQHLLKSLHE
                     STPH"
     gene            complement(5648..5839)
                     /locus_tag="PKZ49_06225"
     CDS             complement(5648..5839)
                     /locus_tag="PKZ49_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HND36194.1"
                     /translation="MKIFAIVLLILILYSLGSGLYFMLKDDGSSTRMVKSLTIRIALS
                     LILFITLMISTYFNYIYQG"
     gene            6028..6990
                     /gene="miaA"
                     /locus_tag="PKZ49_06230"
     CDS             6028..6990
                     /gene="miaA"
                     /locus_tag="PKZ49_06230"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HND36195.1"
                     /translation="MPKFAPLNKLPPAIAILGPTASGKSNLALSITDHFPIEIISIDS
                     AQVYRYMDIGSAKPDPQILAKIPHHLINLIDPDQQYSAAQFRHDALLTMKEITLRGNI
                     PLLVGGTMLYFKALREGLSDLPPADQQIRKEIEASAAEKGWSTLHHLLQSVDPMSAER
                     IQPNDSQRIQRALEIYYLTGKPMSQQMVSSQPASIPYRLISIALLPSDRKVLHQRIQH
                     RFETMLEMGLIEEVRSILSQFSLTLEMPSMRCVGYRQVYQYLQGQLSLMELREKGAAA
                     TRQLAKRQLTWLRSMDSLTEFDCLANDLVEQVLRYLQTMLNAND"
     gene            complement(7002..8228)
                     /locus_tag="PKZ49_06235"
     CDS             complement(7002..8228)
                     /locus_tag="PKZ49_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36196.1"
                     /translation="MRNVVTAPYKRLVINFHWLICLICLVTITGCLSPIALNRAVIAY
                     DDAVTDVVSQQLLINIVRAHHRQPIHFTGVSNIAATFTFQASAGAAPAAGGLAGSSIL
                     PIFGGSMAESPTISIVPIEGEDFTRRLLTPFPQSGLILLLRQHFDVDLLLRMMAQEVR
                     LQHKPYHSDTFRNKSTDKAGYEMFRRIVLHLSAIQDQNQLYVEPLPLTHTWKIPAATI
                     APDSFQSLQKEFVVHYDSADDTFTLHKQILGPILITNYDPDTLSESERELLNERAREW
                     DVSDVAFDIRAGYYGGEWPMVGIFRLRSFHSILSFLSKALGNETGYHVEKDPRTPPIL
                     NNENPDLTMELIQSNIAPSGVDFSIRWNNQFYAVNTTGPHARWNRDAFQLLFLLFQMT
                     VTDIPRVGVPSITIAK"
     gene            complement(8280..10358)
                     /locus_tag="PKZ49_06240"
     CDS             complement(8280..10358)
                     /locus_tag="PKZ49_06240"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HND36197.1"
                     /translation="MDHSFFNLYHHGFVRVAVGVPDVRVADPVFNVNETIQLMKQAAE
                     QHAALILFPELGVTAYSCEDLFHQQALINSSVEALQSLLDQSKHINIITVVGLPLQIN
                     QLLFNCAVVIYGGRILGVVPKTYLPNYREFYELRQFSPASAASSDVISLCQQTDIPFG
                     ERLLFSANNQPSFKFFIEICEDLWVPIPPSSQAALAGASILLNLSASNITMGKHEYRQ
                     NLVANQSARCLAAYLYATAGVGESTTDLAWDGHALVYENGTCLAESERFRQSSQLILA
                     DLDLDRLKQERMRQTSFSQSVQYYKESIRQFREIKFDIYFPEVERLLCQRHYSRFPFV
                     PSDAHQRDKHCLEAFNIQVQGLIKRLKHTGIQKIVVGISGGLDSTHALIVACRVMDEL
                     HYPRHHVLAYTLPGYATSGQTKANALALMNSLGCTSHEIDIRPSCEQMLKDIDHPFSR
                     GESVYDTTFENVQAGERTNHLFRLANYHHALVLGTGDLSELALGWCTYGVGDHMSHYA
                     INASMPKTLIQFMIRWVADAQLFNAETSELLNAILATEFSPELIPGNTTDNQPYQKTE
                     SIIGPYELQDFHLYYTIRYGYLPTKIAFLAYCSWRDRSVGNWPGIPQDRQNQYTIGEI
                     KHWLKVFLYRFFQTSQFKRSCIPNSPKVGSGGSLSPRGDYRAPSDSEATIWLSNAELI
                     PDIAPPDILK"
     gene            complement(10799..11011)
                     /locus_tag="PKZ49_06245"
     CDS             complement(10799..11011)
                     /locus_tag="PKZ49_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36198.1"
                     /translation="MDRELSFDADNFDLDLSELKELEDIVADGMIPDSRIVKIIDEIE
                     FAKEDNFDVFEDLEDTEISLSRFNLD"
     gene            11306..>12252
                     /gene="lplT"
                     /locus_tag="PKZ49_06250"
     CDS             11306..>12252
                     /gene="lplT"
                     /locus_tag="PKZ49_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HND36199.1"
                     /translation="MERGFYTIMAAQFFSSLADNALLVVAIALLIDLNAPAYLTPMLK
                     FVFVLFYVVLAPFVGAFADSMAKGRVMFVSNTIKIAGCSLLFFAMHQYFALAAYAIVG
                     LGAAAYSPAKYGILTELLPPEQLVTANGWIEGLTVASIVLGTVLGGVLITPSVATLIL
                     SFDFPLIDTGIDSAVEASIIIIGVFYAIAALFNLFIPDTGVDHRILKFNPLFLVHEFS
                     HCVKLLWADKLGQISLAVTTLFWGAGATLQFIVLKWAEAALNFPLNQAAQLQGVVALG
                     IAIGAVLAAKIVSLRQSVKVIPLGILMGIVVMVMTLVNNL"
BASE COUNT         3648 a         2707 c         2485 g         3412 t
ORIGIN      
        1 ttaaatcacc gaaagtatgt tcatcaataa aactcaccaa tccatagagt tccaacagcg
       61 aattttgtag tggtgtcgct gtaagcagta gcttatgttt tcccgccaat gccatcttca
      121 atgtatttgc tatgacattg gatggcttat aaacattgcg cagtctatgc gcttcatcaa
      181 taacaaccag atcccaaggt attgcatgaa tatctgcagc cttacttttt gcaaactggt
      241 atgagcaaat tatgatttca ggtgattcaa atggacgaaa ctggccttgt ttgattgcag
      301 cgttatagga ttttgcttcg aggatatagc gcgggagaaa gaatttctcg ttcaactcct
      361 ggtgccattg tttacacagg tttgaaggtg taatgatcaa aatacgccgt ttacgctcgg
      421 cccatttttg agacagcacc aatccagctt caatagtctt gcctaatccc acttcatcag
      481 ccagtagcgc acccttagaa agtggcgagt taaaagcaaa caaagctgca tcaacttgat
      541 gcagattgat atccacttga gcactggcta ctgcacctgc taatttttca gcgctatctg
      601 ttggaaaacg acgcgtcagt tcgtgcgcaa agtattttgc ttggaaagct gtaatcatta
      661 gccgaagtct atcaccaagt attccaattc atatcgacca gaatgattta aaaagaattt
      721 ccgctatttt aagtatatag aaatgtactg tacatattca gaataattca atttgtggga
      781 gtttatatca gactatttct ttgaacctga tctatccagg tggggtgtta atagtcacca
      841 caaacccgca ttccatcatc tctttctgct aacagtcctt gaattgccat tcttgtaacc
      901 aagggtaccc agtgagatta gctgtcatgg tattttttcc atgaactggt cagcagcgaa
      961 ttaatatacc tttaaaaaat taccgacttt atcagctaat gattagtaat tactacaagg
     1021 atttacgatt taaacgcttg aaaaataatt gcttcacaac ctctgaggtg attacataga
     1081 gtagggtaat gatcataatc gcgatcatga ctggtaatgg gagtgggaca aaactgaata
     1141 agcttcctat cggtacataa ggtagcaata aggcgatcaa tgcaataccg ataacactcc
     1201 aaataagaaa acggccaggt tgaccacgat aaaatggctt ataggtgcgg atgacgaaaa
     1261 taatgagcaa ttcggtcaat agcgattcga caaaccagcc tgtacgaaat atcgctttga
     1321 cctctcctgc caaataaagc aatacgccaa atgtgagtaa atcaaacacg acactaacca
     1381 aaccaaaagt aatcataaaa tttcgaatca gcgtcacatc ccaacgatgg ggagtacgtt
     1441 cccagttacg atctactcgg tcacctgcaa tgcccatcgc tggaatatcg gataaaaaat
     1501 tattgagcaa aatctgctta gcgagtaaag ggagaaaagg aagaaatagc gaagccaatg
     1561 ccatactgat catattgccg aaattggcac tggtcgtgat gaaaatatac ttaagcgtat
     1621 tagcaaaggt atggcgacct tcatcgatgc cttggcataa cacattgaga tcatgcttca
     1681 gcaagacaaa atccgccgct tctctggcaa catcggcagc attttcaacc gatatgccga
     1741 catcagcggc atgcaaagcc ggtgcatcgt tgataccatc gcccagatag cccactacat
     1801 gccctgtctt ttgtaatgcg gtaataatgc gttctttctg gtttggatca acttctgcga
     1861 aaacccccgc ttgcggtgca agatgccata gggcttcatc tttcatatcg gcgagtgctt
     1921 gacccgttac aatcgtttgg gtatcgattc ccaccgcttc agccacatgg caagcgacat
     1981 aacgattatc tccggtgatg attttaagct ggacacctaa ttgcgttaac gccttgagtg
     2041 tatcggtaat tccagcttcc ggtgggtcaa aaaataataa aaaaccaatc caaacaaagc
     2101 tatgttcatc gtctcgtgtg taattgttat tgattgcaac cgatttctgg gccactgcta
     2161 acacgcgata accttgcata ctccattgtg tatagtgttg gagcagttgt tgctgatgtt
     2221 cggaagttaa tggttcaatg tgagaagcta attgaatttg atcgcaaatt tctataatcg
     2281 attgaaatgc gcccttgcaa atcatcgttg cagttgcgtc catttcatgt ttagccaccg
     2341 tagtaaggcg cttacggaca aaatcatagg gaatctcatc taatttttga taacctgtta
     2401 gagtgatgtt cgattgttca cccgctacga taatagcttc gtccagtaaa ttgatgatac
     2461 cggtttgtaa cctggaattc acataagcta attgcaatac taatttgctt gtttgcccca
     2521 agctatcgag cgcttgaccg agctgaacga ctcctttggt taaagtgcca gttttatcgg
     2581 tacataacac gtccatactc ccaaggttct cgatggcatt gagacgtttg acgatgacac
     2641 cttgcgctgc catcgcttta gcacctcgtg ccaaggtaat ggtaagaatg gcaggcaaca
     2701 actcaggcga cagccctact gccagggcca gcgcaaataa tactgtttca atcgtcgggt
     2761 gttgtaagac gatattgatc gccaggattg cgatgaccat tatcaccatg atacggacca
     2821 gcaattgccc aaaatgccgt aatcctcgct caaactccgt ttcaggggga cgtaatgaaa
     2881 gcgtttcagc aattttgcca taagcggtcg tcaggcctgt ttgaatgatg agtgcttttg
     2941 catacccact tcgcactgaa gtacccataa aaatacaatt attgcgatcc actagacttg
     3001 cctctggcgc tgagattcct ggtttttttt ctacagggaa tgtttctccg gttaataagg
     3061 cctgtgcaac atgaaaatcc ttcgcttcaa gtagcattcc atcagcagga atcaaattac
     3121 ctgcggataa cacaacgata tctcccggta cgatggtatc agagggaacg acgagggttt
     3181 gtcctccacg gagcactgtt gatctggtcg caattttctg gcgtagtttc tcaacagccc
     3241 gagttgcgcg gtgttcctga ataaaactga gcactgctgt catcccgaca atcataagca
     3301 cgatcaaggc atccaaccaa tctttgacca gcagcgcaat gattgcagca aaaattagga
     3361 ttaaaaccaa agggctgcga aattgattta aaaaaagctg taaagtgctt ccggaaaagt
     3421 gattagtttt taaccgattt gcgcccgtgt gcattaagcg atctgctgcg atggctggat
     3481 cgagaccctg agagttacta cccagcgatt tcatcataga atcagccggt tgactccaat
     3541 aaaccgcaat ctcaggattc ttttccatag cgttgattat tatcgattag gcaatagcat
     3601 taacagcaaa acgccagaat tttctggaaa attttgctgc caagactaaa gaaacattta
     3661 cttattctaa gcttaaaatg tgctgaacct caatgaggcg tagattcatg caaacttttc
     3721 agtaaatgct gtgtaatgtt ttgtgcaagc acttgctcat cgaggaatac tagccccata
     3781 ttctcttttt ccagccttgc agcggcttct ggatcatggg tgctaattaa aatttcagct
     3841 tgaggattta atattttggc ggtttcaacc atttgctgaa tgcgcaatac atcagcagcg
     3901 gtaatgatca ttgccccagc tcgagcaata tgggcttgaa ttaataccgc tggatcagcg
     3961 gcatctcctg ctactgcatg cgcatgatgt tttctgagct gctccacaat ttctcgattc
     4021 tgatcgacga ctacataatg cgtaccttgg ctcgcaaggg tttcactcac acgcttaccc
     4081 actgcaccat agccaacgat gaccacatga ccggtgacat gcgttgatgc aaccgatgtt
     4141 ggtaattctg ctaacggatc atcagtccgc tctagtcggc gggcaagctt agagcgagaa
     4201 cggatccaat tttggatcgg ttcaacggtt tcaaagacca gatgatttaa ggtaatggaa
     4261 ataaacgcgc cagccaatat caagctatac ccctccagtg gtaaaagctc taatgaaacc
     4321 ccgagtgaag caagaataaa cgataattca ccgatttgag ataagctcac cgataccgtc
     4381 aaggccgtgt tgagtggata gcgaaatgcc agcacgagaa taaaggcagc taacgattta
     4441 ccaaacagaa tgatagcaac cacaatcagc acgtgcagcg gttcttcaat caaaatattg
     4501 ggatcgaaca gcatacccac tgatacgaag aacaatacgg agaaagcatc tcgcaaaggt
     4561 agcgattctt gcgcagcacg gtgactcaag cttgattccc gcatgaccat gccagaaaag
     4621 aaagcaccta gcgcaaatga aacatcgaaa agcttagcag caccgtaagc aataccgacc
     4681 gcgacggcga tgacgcatag aataaataat tcattggatt tggtgctagc aacgtgccac
     4741 aacaaccaag gaaatagtcg tttaccgaca atcaacataa aaccaataaa tatcgatact
     4801 ttgacaaaag taatcgctaa tacaatcaac aaatcggtta tgttattgct ggtgctcgaa
     4861 tcatctccct gccctcccaa ccaacccgca agggggggaa gcaatactaa agccagcact
     4921 acaactaagt cttcgacgat tagccagccg acagcaattc gaccattaat cgatttcatt
     4981 aaaccacgtt gctcgagtgc gcgaatcaat acgaccgtac ttgctactga gagtgctaga
     5041 ccgaatacga tacttgcacc taaattccac tcccaagtca tcgcaacact cgcaccaagc
     5101 aaagtcgcta caatgatttg agcaattgca cctggtagtg ctatttttct aactgctaat
     5161 agatcactga gtgaaaaatg caaacccacg ccaaacatca gcaaaataac gccaatttct
     5221 gctaattgat tagataaagc aagatcggct acaaatcctg gagtaaaggg accgatcatc
     5281 atgccagcaa ccaaataacc gacgagtacc ggaagcttta aacgatttgc gagcataccc
     5341 atgatcaagg caagacccag actaacagcg atcgtcgtaa ttaaaattgt gctatggggc
     5401 atagacgaga aggagtttta tggttgattt aactgactat tttgaatcga ctaagctttt
     5461 tgttgttttt aagatcgaat gcgacagctt tgtctggtcc aacaggtttg ttattcttag
     5521 taagccctag aatagcttca tcattactga gtctaatctt ctaggccagg agagtcgcta
     5581 gcaaacgcca gtcaatgatc gcttccacta atatgtaaac attggaagcg ctttggcttt
     5641 ctgcttttca tccttgataa atataattga aatacgttga gatcatgagt gtaataaaca
     5701 gaatgagaga tagtgcgatc cggatggtca gtgatttaac catgcgagta ctactcccat
     5761 catcttttaa catgaaatac aacccagagc caaggctata aagaatgagt atcaataata
     5821 ctatcgcaaa tattttcact acaatttcct ataaaaatgg aatgatatct cactgatgcc
     5881 attcattaac tgcggaatca caatcgaaca taatcatcag ttttttccta ttgtaactga
     5941 taacgatcaa ttatgattgt tacagtccat aacaacttac cccaatttag ttatgatcac
     6001 gttactcaag aaaaattgca ttaactgatg cctaaattcg ctccattaaa taaattacct
     6061 ccggccattg ctatcttggg acctaccgca agtggtaaat ctaacctagc cctatcgatt
     6121 accgatcatt tcccaataga gattattagt atcgactctg cgcaagtcta tcgctatatg
     6181 gatattggta gcgcaaaacc cgacccacaa atcttggcta aaattcctca tcatctgatc
     6241 aatttaattg atccagacca gcaatattct gctgcgcaat ttcgtcatga tgcacttctc
     6301 acaatgaaag aaataacgct aaggggaaat attccattgc tagtcggcgg taccatgctt
     6361 tatttcaaag cgttgagaga aggcctgtct gatcttccac cggcagatca gcaaattcgt
     6421 aaggaaatcg aagcaagtgc tgcggaaaaa ggctggtcta ccctgcatca cttgctgcag
     6481 tcggttgatc ctatgagtgc tgaacgcatt caacccaatg atagtcaacg aatacagcgc
     6541 gcgctggaaa tttattactt gactgggaaa cccatgtcac aacagatggt gtcttctcag
     6601 cctgcatcga ttccttatcg attgatttcc attgcattat tgccatcgga tcgcaaagta
     6661 ttacaccagc gaattcagca tcgttttgaa acaatgttgg aaatgggatt aatcgaagaa
     6721 gttcgatcga ttctgagtca attttcgctg acgctagaaa tgccatcgat gcgctgtgtg
     6781 ggctatcgac aagtttatca gtatctacag ggtcaactca gcttaatgga gttacgagaa
     6841 aaaggcgcag cagcgacacg tcaattagcc aaacgtcaat tgacttggtt acgtagtatg
     6901 gattcgctta ctgaattcga ttgtttagcg aatgatttgg tcgaacaagt tttacgctat
     6961 cttcaaacca tgctcaatgc taatgattaa gcttaatttt gttattttgc aatggtaata
     7021 ctcggtactc caacccgtgg aatgtcagtt accgtcattt gaaatagtaa aaataacaat
     7081 tgaaaagcat ctcgattcca gcgagcatgg gggcctgttg tattaaccgc ataaaattga
     7141 ttattccagc gaattgaaaa atcaacacct gacggcgcta tatttgactg aatcaattcc
     7201 atcgtaagat ctgggttttc attattcaag atgggtggcg ttctcggatc tttttcaaca
     7261 tgatagcccg tttcattacc tagcgcttta ctgagaaaac tcagaataga atgaaaactc
     7321 ctcagtctaa aaattccgac catgggccat tccccgccat agtaaccagc ccgaatatca
     7381 aaagcgacat cgctcacatc ccattcacga gcacgttcgt tcagtaactc tcgttcactc
     7441 tcagataacg tatctggatc atagttggtg atcaaaatag gacccagaat ttgcttgtga
     7501 agcgtaaacg tatcgtcagc actgtcatag tggacaacga attctttttg caacgattgg
     7561 aaactgtctg gcgcaatggt agctgcggga attttccaag tatgcgtcag aggcagtggt
     7621 tcaacgtaga gctgattctg atcttgaatg gctgataaat gcagaacgat gcgacgaaac
     7681 atttcatacc cggctttatc agtggattta ttgcgaaacg tgtcactatg gtaaggttta
     7741 tgctgcaaac ggacttcctg cgccatcatg cggagtaaca gatcaacatc gaaatgctga
     7801 cgcagcaata atatcaaccc actttgcgga aacggtgtaa gcaatcgccg cgtaaaatct
     7861 tctccttcga tgggaacaat gctaatcgtc ggactttctg ccatactccc accaaaaatt
     7921 ggcagtatcg atgagccggc caacccacct gcggccggag cagcaccggc acttgcttga
     7981 aacgtaaatg ttgcagcaat attagaaaca cctgtgaaat gaatgggttg ccgatgatgc
     8041 gcgcgaacga tattaatcaa taactgctgt gataccacat ccgtcaccgc atcatcataa
     8101 gcgatcacag cacgattcaa cgcaatcggc gataaacaac ccgtaatcgt aaccaagcag
     8161 atcaagcaaa ttaaccagtg aaagttgatg actaaacgct tgtatggggc agttactacg
     8221 tttctcatcc cagtcgagga atcatacttg cgaataaaca tatgagcgtt ggtaccttat
     8281 catttcagaa tatcaggggg tgctatatct ggaatcaatt cagcattact taaccaaatc
     8341 gttgcttcac tatcgctggg tgcccgataa tctccccggg gtgagagtga gcctcctgag
     8401 ccaaccttgg gtgaattcgg tatacaactg cgcttgaatt gactcgtttg aaaaaatcga
     8461 tatagaaaaa ctttcaacca atgtttaatt tctccaatcg tatattggtt ctgcctatct
     8521 tgcggaattc caggccagtt tcctacagaa cgatctctcc aactgcaata ggctaaaaaa
     8581 gcgatttttg tagggagata accatatcga atagtgtaat aaaggtggaa atcctgtaat
     8641 tcatagggac caatgatgga ctctgttttc tggtaaggct gattatccgt ggtattacca
     8701 ggaatcaatt cagggctaaa ttctgttgct aagattgcat tgagtagttc actagtttca
     8761 gcattaaata attgcgcatc tgcgacccaa cgaatcataa actgaatcaa agtcttaggc
     8821 atactcgcat tgattgcgta gtgagacatg tgatcaccca ctccataagt acaccacccc
     8881 aaagctaatt cactgaggtc gcctgttccg agtaccaatg catgatgata gttggccaag
     8941 cgaaaaaggt gattggttcg ctcccccgct tgtacatttt caaaagtcgt atcatagaca
     9001 gattccccgc gactaaatgg gtgatcgata tctttcagca tttgctcgca gctaggtcga
     9061 atatcgatct catgcgacgt acaacccaaa ctattcatca atgcaagggc attggctttt
     9121 gtttggccac tcgtcgcata accgggtaac gtataagcta gcacgtgatg gcgcgggtaa
     9181 tgtaactcgt ccatgactcg gcaagcaaca atgagcgcat gcgtcgaatc aagcccaccc
     9241 gatatcccca cgacaatttt ttgaatgccg gtatgcttta atcgtttaat taaaccttgc
     9301 acctggatat taaatgcttc taggcaatgt ttatcgcgtt gatgcgcatc ggatggtacg
     9361 aatgggaagc gactataatg ccgttgacaa agcaatcgct cgacttccgg aaagtagata
     9421 tcaaacttaa tctcgcggaa ttgtcgtatc gattccttat aatattgaac actctggcta
     9481 aaactcgtct gccgcatacg ctcttgcttc aagcgatcca gatctagatc agccaggatc
     9541 aattgtgaac tttgtcggaa gcgttctgac tcggccaagc aagttccatt ttcataaacg
     9601 agtgcatgcc catcccaagc aagatcagtc gttgattcac caacccctgc agtggcatag
     9661 aggtatgctg ccaggcaacg cgctgattga ttagccacta aattttggcg atattcgtgt
     9721 ttacccattg taatgttcga agcagacaga ttgagtaaga tggaagcgcc agccaatgca
     9781 gcttggctcg aagggggtat cggcacccag agatcttcac aaatttcgat aaaaaattta
     9841 aaagaaggct gattatttgc actaaatagg agccgttctc caaagggaat atcggtttgc
     9901 tggcataatg agattacatc ggatgaagca gcgcttgcag gtgaaaattg ccggagttca
     9961 taaaattcac ggtaattcgg aaggtaagtt ttaggtacaa cccccaagat acgaccgcca
    10021 tagattacaa ccgcacaatt gaataaaagc tgattgattt ggagcggtaa cccaacaact
    10081 gtaatgatat tgatatgctt cgactgatcg agtaatgatt gaagcgcttc aacactacta
    10141 ttgattagtg cttgttgatg aaataaatct tcacaggaat aagctgttac ccctaattct
    10201 ggaaaaagta tcaatgcagc atgctgttca gcagcttgct tcatcaattg aatcgtttca
    10261 ttgacattga ataccggatc agcaactcga acatccggaa ctcccactgc gacacggaca
    10321 aaaccatggt gatacaagtt gaagaaagag tgatccatcg gtatcaaatc cgtgatgagt
    10381 aggccttaat tagcaagcgt aagcttttac actttcattt attagcaagc aagaaatggt
    10441 aggccaatat gactatttac cctattgttt agctaatttg aattaatttc aacaaattat
    10501 cggggtgaat tttcctgccc tacccctatg attgactcta ccgaaagtca aaattagcgt
    10561 caactgatgt tttacaagct ttatcgtttc tcagtcagta aaatgactgg aaaacaatcg
    10621 atggattaca agatgggagt aacaacacgc gattaatacg actagctgcg ccacttcatc
    10681 gccgtatcat tttaaattta caaagcaaaa tcgctactaa cgattagtat cgtagactag
    10741 cgattcaggc cagttgtagc ataagaaacg ttaaactagc cttacctatt taagtcattt
    10801 agtctaaatt gaatcgactt aacgaaattt ctgtatcttc aagatcttca aaaacatcaa
    10861 aattatcttc tttagcaaat tcgatttcat caataatttt aacgattcga ctatctggaa
    10921 tcatgccatc tgctacgata tcctctagct ctttaagctc acttaaatct agatcgaaat
    10981 tatcagcatc aaaagaaagt tctctgtcca ttttgcaaac ctccttaaca ctttatttga
    11041 agacaatata ttaatatcac gttcaattac aaaacaaagc agaaacaatt agtgttttac
    11101 agtacttatt agacttttga atgacttcga tcgttctaac aattaacaaa tttactgatg
    11161 atgtatataa atttccaaaa gaaaaataac ccaggaagat gcttaatcct gatcagcttg
    11221 ttttcacata ggaatattaa tacacatttt gttcatatca atttcagata ctatttttaa
    11281 aatattaact ttaaggagca ttagcttgga acgcggattt tataccatca tggcggcaca
    11341 atttttttcg tcactggctg acaatgcgct gttagtggtt gccattgcat tattaatcga
    11401 tctcaatgca cccgcatacc ttactcccat gctcaaattt gtatttgttt tattctatgt
    11461 agttcttgca cccttcgtcg gggcatttgc cgattccatg gcaaaaggac gcgtgatgtt
    11521 cgtcagtaac acgattaaaa ttgctggttg ttcactactt tttttcgcga tgcatcaata
    11581 ttttgcattg gcagcttatg cgatcgttgg actcggagct gccgcatatt caccagcaaa
    11641 atatggcata ttgaccgagt tattgccgcc agaacaactc gttactgcca atggttggat
    11701 tgaagggctc accgtcgcat cgattgtgct tggaacagtg ctgggcggtg ttttaattac
    11761 gccatccgtt gctacgctga tactttcttt tgattttccc cttatcgata ctgggattga
    11821 ttctgccgta gaagcaagca ttatcatcat tggggttttt tatgcgattg cagccctatt
    11881 taatttattc atccccgata ctggggttga tcatcgcatc cttaaattta atcccctttt
    11941 cctagttcat gaattctcac actgtgtcaa attgctctgg gctgataaat tgggtcaaat
    12001 ttctctcgca gttactaccc tattctgggg tgcaggtgca acgttacaat ttattgtatt
    12061 gaaatgggct gaagcagcac tgaattttcc actgaatcaa gcagcacaat tacagggggt
    12121 tgttgcactc ggtattgcga taggtgctgt tttagctgcc aaaatcgttt ccctgcgtca
    12181 atcggtaaaa gtcattccat tggggatact gatgggcatc gtcgtgatgg tcatgacact
    12241 cgtcaataac ct
//
LOCUS       DAOQKQ010000072        12228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_2425_length_12228_cov_5.576275, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000072 DAOQKQ010000000
VERSION     DAOQKQ010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 12228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12228
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2425_length_12228_cov_5.576275"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /gene="gcvT"
                     /locus_tag="PKZ49_06255"
     CDS             complement(<1..1033)
                     /gene="gcvT"
                     /locus_tag="PKZ49_06255"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HND36200.1"
                     /translation="MLKMTPLNQAHRDMNAKMVDFGGWDMPLHYGSQLDEHHKVRRDA
                     GMFDVSHMLVVDIEGSDVRNFLKRLVANNVDKLTLPGKALYTCMLTPQGGIIDDLIIY
                     FLTESWFRLVVNAGTADKDIAWIMQQRDSLAPQLQIKPRRDLAMIAVQGPNARAKVWQ
                     VLPGAQAVTENLKQFQSATFENYFIARTGYTGEDGFEIVLPAQDAVDFWKKLHAAGVA
                     PAGLGARDTLRLEAGMNLYGQDMDETTHPLESGLAWTVDLKSERDFIGKQALLNAPNS
                     RQLVGLLLLDKGVLRGHQPVITQHDSAGSTGGEITSGGFSPTLNQSIALARISSQTAI
                     GETVHVVVRD"
     regulatory      complement(1078..1185)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(1643..1861)
                     /locus_tag="PKZ49_06260"
     CDS             complement(1643..1861)
                     /locus_tag="PKZ49_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36201.1"
                     /translation="MALMNQQTAMPTISSALTQGKDSQVGRVATQTKIGCTQREKASL
                     ASAEANPAEASFTRMAKVGSIYCGDSMT"
     gene            complement(1871..2017)
                     /locus_tag="PKZ49_06265"
     CDS             complement(1871..2017)
                     /locus_tag="PKZ49_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36202.1"
                     /translation="MGPTENTAATMQRPKAVTCTTTPVYMRVRTQTHVPGVGVCPERH
                     VATA"
     gene            2553..2771
                     /locus_tag="PKZ49_06270"
     CDS             2553..2771
                     /locus_tag="PKZ49_06270"
                     /inference="COORDINATES: protein motif:HMM:NF022603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HND36203.1"
                     /translation="MEASKTSTKKSKYQTSENNGEVSIKEDRQLDWHNRIATAAYYKA
                     EARSFEPGHDIEDWLAAEAEEKDEQLTC"
     gene            2752..3150
                     /locus_tag="PKZ49_06275"
     CDS             2752..3150
                     /locus_tag="PKZ49_06275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HND36204.1"
                     /translation="MNNLPVETRSTAEVRESISKQSDKYAALILSEDGIIQDCSKEGA
                     RLLGYMPENLIKLHITKLLPKLLEIDLLKAHQINPYLRFLSRIGHRFELIEMNGDRMI
                     GQLFFSDLENEGGHQLVVLINPIPEKKQLH"
     gene            complement(3440..4096)
                     /locus_tag="PKZ49_06280"
     CDS             complement(3440..4096)
                     /locus_tag="PKZ49_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36205.1"
                     /translation="MPKIKISFEIILPVLLIILLLVPYTLNRIGHSMYGIKQFPPVIL
                     MKLPVDHSAVNLPQAVKELQSFNVALELDTSELIKRTNDIIGKSSHGTGLQGLTGQVL
                     PGMQAEMAGANFQIDNPGPHVELFSSNRQTLWTWTVIPKKTGRQSLSLLLHLETMNGG
                     QQAKKVVELAEIQMYVENNPEEWLKQNAWWMILISIALGYVLVKYRSDKKHKQPEEPK
                     "
     gene            4196..4975
                     /locus_tag="PKZ49_06285"
     CDS             4196..4975
                     /locus_tag="PKZ49_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HND36206.1"
                     /translation="MNSNPTIPILVAHRGYPACYPENTLVGYEVALQAGACYVELDIQ
                     LSARHTPFLCHDDNLKRLTGIDQNITELADEIIKTLSIAYPQSTIADEATVSPTKTAP
                     FADLLSFCLLLEKWPQAKAFVEIKSESIVKFGLELTVETILQVLQPYREQCIIISFDW
                     QAVALARKKGMQQIGWVIPRWDDAHAKIADTLAPDYLFCSIWRIPPSIEERWKGPWQW
                     VVYTINDSQTALQYAQQNIGMIETDAIGVMLSDPTLKQHAC"
     gene            4965..6161
                     /locus_tag="PKZ49_06290"
     CDS             4965..6161
                     /locus_tag="PKZ49_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HND36207.1"
                     /translation="MLASQSYDLVVIGGGIQGTAIAQAAAANGHHVLVLEKTGLAAGT
                     SSRSSKLIHGGLRYLEQGHFGLVRESLRERSQLLKLAPDLVKLSPFYIPIYQSTSRGK
                     WTIRAGLTLYGLLAGLGPASRFRSVPRADWAKLDGLSTAGLSHVFQYWDAQTDDRLLT
                     QALMHSAMKLGAELCCPAEFMQANLTQDRCELTYRWQNQSHHCTAAVIINAAGPWVNQ
                     ILHNVTPSPPTLPIELVQGTHLVLEGKPGSGCYYLESPVDQRAIFVLPWYEHTLLGTT
                     ETIFTGLPEACHPLAEEEAYLIECFRHYFPNRKVVVREKFSGLRVLPKAQGSAFSRSR
                     DIQLLCDNVSQPRIITLYGGKLTVSRATAQKVLTMAASSLPQRHPKANLAELPIKLPP
                     SASEYV"
     gene            6154..7605
                     /locus_tag="PKZ49_06295"
     CDS             6154..7605
                     /locus_tag="PKZ49_06295"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HND36208.1"
                     /translation="MSNQAVTLAIDQGTHLSRALLFDPGGEVIAQFSEPVEIFHRSSV
                     EVEQDPDEIIDSIHSIVSQSLQFAAQHQKRISHIGLATQRSSVVAWHKTTGKALSPVL
                     SWQDRRAADWLMSFAAHQATIIERTGLRLSPHYGVSKLNWLRKNIPAVIAAEAQQQLA
                     FGPLASFILFHLLEGNPLVVDHANAARTLLWNITSCDWDDVLLKLFEIPRPYLPQCYP
                     IRHCYGYLKNHSIPITAVNGDQTAALCAAAEVSQEGVWINMGTGAFILKSTGCQLIRH
                     RKLLSGIADSDAQEKRYVLEGTVNNAGSAIDWLSEHESMPHPYQKLDAALRSITDPPL
                     FMNTISGLGSPWWGKGPAPQFHDLDGKPLTNLPADHRLVAVVESILFLIQANLDCMAE
                     THLPIKGITISGGLAQSDALCQKLADLSGLTVQRPALVEATAQGIAWLAAGKPSGWHK
                     QGKIDCFSSKATTARQNVINERYQKFVSILQSN"
     gene            7639..8040
                     /locus_tag="PKZ49_06300"
     CDS             7639..8040
                     /locus_tag="PKZ49_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HND36209.1"
                     /translation="MAITGMNHFTVLTTDLDKSKAFYVSLLGLTEGYRPPFEFPGAWL
                     YCGDQAILHIMAGRPIPATIHGIIDHIAFSACNLQSVIDRLNQHNIAYTLNRLKGLES
                     WQLFCADPDGAKVELDFAASEPEPQNVKQTK"
     gene            complement(8060..10807)
                     /locus_tag="PKZ49_06305"
     CDS             complement(8060..10807)
                     /locus_tag="PKZ49_06305"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HND36210.1"
                     /translation="MWRYLLIITTFIISLSIMSLGHAQQPMQSLPANLTQITSVEGIT
                     EYQMPNGLKVLLLPDDSLDTVTVNIVYLVGSRDEGYGEAGMAHLLEHLLFKGTERHPD
                     IKNEFTKRGARWNGTTSYDRTNYYEVLTASTENLDWAISLEADRMINSRIEKADLDSE
                     MTVVRNEFEAGENNPFGVLSERMASTAFVWHNYGRAIIGSRSDIENVPIERLQDFYHH
                     YYQPDNAVLVIAGKFDETAALNSISKHFGAIPHPKRQLRKTYTVEPTQDGERNVMLRR
                     VGDTQIAATLYHIPAAAHPESAAIDLLVSIIKQVPGGRLHKSLVESKLATAIFGFDRQ
                     LRETGFVYFGANLLPTMSLDHASNTLISTIETLAAHPITAEELERARTRLLNKIEQLL
                     TNTRNLATTITEFIAMGDWRLLFLHRDRIQQVTLEDVQRVANTYFKPSNRTLGLFIPT
                     KTPDRAEIPAVTDLALMFKEYRGNAMLSLGEAFEATPENIEAKLIRTQLPGGMKLALL
                     PKKTRGNRVVVAFNLHWGDEQSKMNRATACGMTSAMLSRGTQRYTREQLRDAFDRLKV
                     HIDMTADGIAFETTGDHLTDALQLVAEMFRNPIFPEKEFEQLKLAILSSIENQKSEPA
                     AQAGIQLERHLMPFASAHWLYTPTIEERIERINQVSLDDVKRCHQDFYGSTDAELAIV
                     GDFEPKAITETLQQSLGDWKSPKPYQRISTPYQDIKAIDLNLETPDKANAVYQAGMNL
                     KIRDDHPDFPALVLGNYLFGGSADSRLWRRVREQEGLSYSVISSLTASAFEARGDFKI
                     FAIYAPQNRDRVEQAVDDEFTRVLKDGFTAEEVELAKAGYLEARRIARGQDKALAERL
                     VNYLHRNRTLQWDIAFEKNIAALTPELILTALRRHFDMTRLTQVKAGDFANTLSAKSP
                     Q"
     gene            complement(10897..11793)
                     /locus_tag="PKZ49_06310"
     CDS             complement(10897..11793)
                     /locus_tag="PKZ49_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HND36211.1"
                     /translation="MPTPPYLQKLVIIGVGLIGGSFALALRNAGLVKQIVGLGRGQEN
                     MLRARELGIIDEIAITPAAALAEADFVMLSTPVGQIQRVMAQIEPYLNKQTIVSDVGS
                     TKQNVIEAAQQQLTNHLAHFIPAHPIAGTEFRGAEAADPLLFKQKHLIITPLKENNEQ
                     AINLVKVLWESCGAKVSIMSAEQHDQMLAIVSHLPHMLAFTLMNHIQTCTDFDPNYVL
                     SYAGSSLRDCTRIAASSPEMWRDICLANRVQLLAELDKYQQALTELREQLANRNGEAL
                     LSLFKKASDLKLQWSNARDEKD"
     gene            complement(11835..>12228)
                     /locus_tag="PKZ49_06315"
     CDS             complement(11835..>12228)
                     /locus_tag="PKZ49_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HND36212.1"
                     /translation="HPELADLMNRIRQPFNVNSLGLIGALAALKDTEFVRRAYELNRA
                     GMAEITNGLRQLGVEFIPSYGNFITLRIRGDEINTAKAYQNLLKRGVIVRPLGVYEMP
                     HHLRVTIGLKSENEKFLSALEQVISNSA"
BASE COUNT         3347 a         2852 c         2650 g         3379 t
ORIGIN      
        1 tatctctaac aaccacatgc acggtttcac cgatggctgt ttgactcgaa atacgtgcta
       61 atgcgatcga ttgattcaat gtcggtgaaa aaccgccact ggtaatttcg cccccagttg
      121 atcccgctga atcatgttgc gtaatcacgg gttgatggcc acgcagcacc cctttatcga
      181 ggagtaatag gccaacgagt tgtcgactgt taggtgcatt taacaacgct tgcttgccaa
      241 tgaaatcccg ctcgcttttg agatccactg tccaggccag ccccgattca agcggatggg
      301 tcgtttcatc catatcttgg ccatataaat tcataccggc ttccaggcgt aacgtgtccc
      361 gcgcccccag tccggccggc gcaacacctg cagcatgcaa ttttttccag aagtcaaccg
      421 catcctgggc aggcaaaacg atttcaaagc cgtcctcacc ggtataaccc gtacgggcaa
      481 taaaatagtt ctcaaaagtt gcagactgaa attgttttaa attttccgtc accgcctgag
      541 cacccggaag cacctgccaa actttagcgc gtgcattcgg tccttgaaca gcgatcatcg
      601 cgagatcacg gcgtggtttg atttgaagct gtggcgcaag actatcgcgt tgttgcataa
      661 tccaggcgat gtctttatct gccgtacccg cattcacaac taagcgaaac caggattcgg
      721 tcaagaaata gataatgaga tcatcgatga ttccaccttg cggtgtcagc atgcaagtat
      781 aaagggcttt cccaggtagc gttagcttat caacattgtt cgctactagg cgctttagaa
      841 aattgcggac atcactacct tcaatatcga cgacaagcat atgcgacaca tcgaacatac
      901 ctgcatcacg tctaacttta tggtgctcat cgagttgcga accataatgg agcggcatat
      961 cccagccacc aaaatctacc attttggcat tcatatcgcg atgtgcctga ttaagcgggg
     1021 tcattttcaa cacggatttt ctcccaaaga taataaatga agtctcattt taactgacct
     1081 cacccatctg tccttttacc tgagagtttt acggtatagt aaagctgatc cgtgtgcccc
     1141 ttcggtggct gaataatgaa ttcagcactc tccagattgc ctgtcacaag cagtttttga
     1201 agttaatctt gatactaacc atacctgagc gattccgggg gagttacgcc ttcggcggca
     1261 ctaaaatgcg cgctttcctg cttggaaatt tatattagca gtttgctaat ataacatacc
     1321 ttggtttagg taataaagca gactattacc gttataattc tagctagctc cccagttggg
     1381 ttacttgaca cccgagatga gcacttacaa tttgctaagc attctaaaac tttgacctta
     1441 gtttatctcg cacttttccg aaaaccagca tttgtctctt ctatgttgct attcagccaa
     1501 tttttggatc taaaaaaacc tgatgtgatg tcgtttagat cgatcaaatg atataaatta
     1561 taaatggggg atcatgagta attccgtatt agatttcatt tgtagcgtaa taaattgcgt
     1621 tatttcagct agctaattct agttaggtca tggagtcgcc acaatatatt gaacccactt
     1681 tcgccatgcg cgtgaagctg gcctcagcgg ggttagcttc agcacttgcg agcgaagcct
     1741 tctctctttg ggtacaacca attttggttt gggtcgcaac tctgcctact tgcgaatctt
     1801 tgccttgggt cagagcagag cttattgtcg gcattgcagt ctgctggttc attagtgcca
     1861 ttgcgcttcg tcaggctgtg gcaacgtggc gttcagggca aactccaact cccggtacat
     1921 gggtctgggt tcgcacgcgc atatacacag gagttgtagt gcaggttacg gctttgggtc
     1981 tctgcatggt cgctgccgtg ttctctgttg gccccatcgt tgtcttcgca acacaagagc
     2041 tttatgtcat cttctgcatt cctgtgccct gtagctgcta gttctcgggg tctgctggga
     2101 ccggatcagc aagaattgga ctcaagtctt acattaccac aaaattagct tgagacattc
     2161 ataagctcac aatcttaatc aatcaactga atgcttcaat cgtgatgagt aaagtctgaa
     2221 acgatttaat ctaatgactc actgattttc taccctgcaa aatcacggta aatcttcccc
     2281 accaaaagat ttgggtaacc aagaaaaatt aataatcgta tcgaatctat tttattctta
     2341 cggccacgct tgcacttcat ctacggtcta gcagctaacg tggcacgcct tagaaagtaa
     2401 ttctccatca aaataacttt gctaaaaatt tttcatatac gtgttagcac gtatatgaat
     2461 tgaaatattt ttttattata ttttcaaaat gaagcggtgc agagaagtga agatttgttg
     2521 tctttttaac ttttttgtat aggagatgaa cagtggaggc atctaaaaca tcaacgaaaa
     2581 aaagtaagta tcagacgtcc gagaataatg gggaagttag tattaaagaa gatcggcagt
     2641 tggattggca taatcgcatt gcgaccgcag cttattacaa agcagaagca cgtagttttg
     2701 agccgggaca tgatattgaa gattggttag cagccgaagc ggaggaaaaa gatgaacaac
     2761 ttacctgttg aaacgagaag tacggcagaa gtgagagaaa gcatttcgaa gcaaagcgat
     2821 aagtatgcgg cgcttattct ctcggaagat gggatcattc aggattgcag caaggaaggc
     2881 gcaaggctac tcggctatat gccagaaaat ttgattaagc tacatatcac gaagctatta
     2941 cctaagctat tagaaatcga tttactaaag gcacatcaaa tcaaccctta tttgcggttt
     3001 ctttcacgca tcggccatcg attcgagtta atcgaaatga acggagatcg tatgattggc
     3061 cagctatttt ttagcgactt agaaaatgaa gggggccatc agttagtcgt tttgatcaac
     3121 cctatcccag aaaaaaagca attgcactaa gccagtttca aatcaactta gtgtaaagcg
     3181 agtcttaagc taagtggcaa taccaaccct caaaacaaga gggaataatc agtcaaggag
     3241 catatatgaa gctagaaatc gtaatgccca ttgtgtcaag tttattgctt attctgatcg
     3301 gattaatgtt actgcctatc gaatgaaggc tatccataga ctggttgaaa tatagcttca
     3361 atcttagtaa tttgttctct aaaaataaga aaggggctag atcggataag cggtatttca
     3421 accgagtgag cgcctaattt catttaggtt cttccggttg tttatgcttt ttatcacttc
     3481 gatattttac taacacgtat cctagtgcaa tactgataag aatcatccac caggcgttct
     3541 gtttaagcca ttcttctgga ttattctcga cgtacatttg aatttctgca agctcaacga
     3601 ctttcttagc ctgttgccca ccattcattg tctcgagatg caacaataag ctaagcgact
     3661 gtcgccctgt ttttttggga ataacagtcc aagtccatag ggtctgacga ttactggaaa
     3721 aaagttctac atgcggtcca gggttatcga tctgaaagtt agcgccagcc atttcagcct
     3781 gcatacccgg taatacctga cccgttaggc cttgtaaccc tgttccatgt gacgatttac
     3841 cgataatatc attggtgcgt ttaatcaact cgcttgtatc caattctagc gcaacattaa
     3901 acgactgcaa ctccttaaca gcctgtggca aattaacagc cgagtgatca accggcaact
     3961 tcatcaggat aaccggtgga aattgcttta tgccatacat ggaatgtcct atacgattca
     4021 acgtataggg caccaatagc aatataatca gcaatacagg taaaattatt tcaaagctga
     4081 ttttgatctt gggcatgctt tctcctgaga agttattttt ctcagatttt agcattcccc
     4141 ctataatatt ggacaaagcc ctttaggttg attactagct caagagaatt aatcgatgaa
     4201 cagcaaccca accattccca ttctagtcgc tcatcgcggt taccccgctt gctatcccga
     4261 aaatacctta gttggctatg aggttgcctt acaagcaggt gcttgctatg ttgagctcga
     4321 tattcagctc agcgctcgcc ataccccttt cttatgccat gatgacaatt tgaagcgatt
     4381 aactgggatt gatcaaaaca tcaccgagtt agcggatgaa atcatcaaaa cgctatcgat
     4441 tgcttatccc caatcaacta ttgctgatga ggcaaccgtt tcacccacga aaacagctcc
     4501 ctttgccgat ttactttcat tttgcttgtt actggaaaaa tggccccaag caaaggcttt
     4561 cgtagaaatc aaatccgaga gtatcgttaa atttggatta gaactaaccg tcgaaaccat
     4621 tttgcaggtt ctacagccct accgtgagca atgtattatc atctcattcg attggcaggc
     4681 tgttgcactc gctcgaaaaa aaggaatgca acaaatcggc tgggtcatcc cccggtggga
     4741 cgatgcacat gcgaagatag cggatacact cgctcccgat tatttgtttt gcagtatctg
     4801 gcgcatacca ccaagcatcg aagaacgctg gaaaggaccg tggcaatggg tcgtttatac
     4861 catcaatgat agccaaacag ccctgcaata tgcacaacaa aatatcggaa tgatcgaaac
     4921 cgatgccatt ggggtcatgt tgagcgatcc cacgctaaaa cagcatgctt gctagccaat
     4981 cctatgattt agtcgttatt ggcggtggca tacaaggaac cgccattgct caagctgcag
     5041 cagcaaatgg ccatcatgta ttagttctag agaaaactgg cctcgctgca ggcacatcga
     5101 gtcgttcaag caagctgatt catggtggat tgcgctatct tgaacaaggt cattttgggt
     5161 tggtacgcga aagtttgcgt gagcgctcac agctgctcaa actagcacct gatctcgtca
     5221 aattaagccc tttctacatt ccgatttacc aatcgacttc acgaggcaaa tggacgattc
     5281 gtgcaggatt aacgttatat ggtttattag caggattagg gcctgcatcc cgatttcgga
     5341 gtgttcctcg cgctgattgg gcaaagcttg atggtttgtc caccgcaggt ctatcgcatg
     5401 tttttcaata ctgggatgca caaaccgatg atcggctgct cacacaagca ttgatgcact
     5461 cggctatgaa attaggtgca gaattatgct gccctgctga atttatgcaa gccaacctaa
     5521 cgcaggatcg atgtgagctg acatatcgct ggcaaaacca atcgcaccat tgtacggctg
     5581 ctgtcatcat caatgccgca ggtccttggg taaatcaaat tctacacaac gtcaccccct
     5641 ctccccccac cttacccatt gaactagtgc aaggcactca tctggtatta gaaggaaagc
     5701 ccggcagcgg ttgctattac ttggaatcgc ctgttgatca gcgcgctatt tttgtactgc
     5761 cgtggtatga gcacacgtta ttaggcacga ctgaaacaat ttttactggt ttacccgaag
     5821 cgtgtcatcc gttagcagaa gaagaagctt atttaatcga atgtttccgc cattatttcc
     5881 ccaatcgaaa agttgtcgtt cgagaaaaat tctcaggttt acgcgtgcta ccgaaagcgc
     5941 agggttcagc tttctcacgc tccagagata ttcaattact ctgcgataat gttagtcaac
     6001 cacgtattat taccctgtat ggcgggaaac tgacagtaag tcgggcaact gcccagaaag
     6061 tgctcacaat ggcagcttct tcgctacctc aacgccatcc caaagcgaat ctcgctgaac
     6121 ttccaatcaa attaccgcct tctgcatccg aatatgtcta accaagcagt taccttagcc
     6181 atcgaccaag gaactcacct atcccgtgcc ctactattcg atcccggtgg agaagtgatt
     6241 gctcaattca gcgaacccgt tgagatattt catcgcagct cagttgaagt ggagcaagat
     6301 cctgatgaaa tcatcgattc gattcacagt atcgtttcac aatcactgca attcgcagca
     6361 cagcatcaga aacgtattag tcacatcggt ttggcaacgc aacgttcgag tgtcgttgcc
     6421 tggcataaaa caacggggaa agcgctcagt ccagtgctaa gctggcaaga tcgacgcgca
     6481 gcggattggt taatgtcatt tgctgcacat caagccacca tcattgaacg gactggcttg
     6541 aggctttccc cccattatgg ggttagtaaa ttaaactggc tcaggaaaaa cataccagca
     6601 gtcattgccg ctgaagcaca gcagcaatta gcgtttggtc cgttagccag ttttatttta
     6661 ttccatttac tcgaaggcaa tcccctcgtc gtggatcacg cgaatgccgc aagaacccta
     6721 ttatggaata tcacgagttg cgattgggac gatgtgctgc tgaagttatt tgaaatccct
     6781 cgaccttatc taccccagtg ctatcccatt cgccattgtt acggttatct aaaaaatcat
     6841 tctatcccca tcacggcggt taatggggat caaacggctg cactatgcgc tgctgcagaa
     6901 gttagccagg aaggggtttg gattaacatg ggtaccggtg cctttattct aaaatcgacc
     6961 ggctgccaac tcattcgcca tcgcaagctg ctcagcggaa ttgctgacag tgacgcacag
     7021 gaaaaacgat atgtgcttga aggcactgtc aataatgcgg gtagtgcaat cgattggtta
     7081 agtgaacatg aatcgatgcc tcacccctat cagaagctgg acgctgcact acgctcgatc
     7141 accgatcccc ccttattcat gaataccatt agcggccttg gctcaccctg gtggggtaaa
     7201 ggacccgctc cacaattcca cgatctcgat ggaaagccct taacaaattt accagcggat
     7261 cacaggttag tcgctgtcgt agaaagcatt ctattcttga tacaagccaa tctcgactgt
     7321 atggcagaaa cacaccttcc catcaaaggc attaccatca gcggtggatt agcccagtct
     7381 gatgctttat gccaaaaact tgctgattta tcaggcttaa cggtgcagcg ccccgcattg
     7441 gtcgaagcaa cggcacaagg tattgcatgg ttagctgcgg gaaaaccgtc tggctggcat
     7501 aagcagggta agatcgattg tttttcgagt aaagcgacaa ccgctcgtca gaatgtcatt
     7561 aacgaacgtt atcagaagtt tgtttccata ctacaatcca actaaaaata ttcaccgcga
     7621 tctttggagg aacctaaatt ggctattact ggaatgaatc attttacggt gctgacgact
     7681 gatttagaca agagtaaagc gttttatgtc agcttgctgg gattaaccga aggttatcgt
     7741 cccccatttg aatttccggg tgcctggctt tactgtgggg accaggcgat cctacatatc
     7801 atggcgggac ggcctattcc ggctacaatt cacggtatca tcgaccatat tgcctttagt
     7861 gcatgcaatc ttcagtctgt gatagatcgg ttaaatcaac ataacatcgc gtatacgctg
     7921 aatcgcctaa aaggacttga aagctggcaa ctattttgtg cagacccgga tggcgcaaag
     7981 gttgaacttg attttgcagc aagcgaaccc gaaccccaga atgtcaaaca aacaaaataa
     8041 gtttatcccg ctcgagttat cactgaggtg atttcgctga tagcgtgtta gcaaaatcac
     8101 ctgctttcac ttgagtcagt ctagtcatat caaagtgacg gcgcaaagca gttaatatca
     8161 actccggtgt caaagcagca atatttttct cgaatgcgat atcccattgc agcgtacgat
     8221 tgcgatgcaa gtaattgact agccgttcag cgagcgcttt atcctgccct cttgctattc
     8281 tccgagcttc aagatagccc gccttagcaa gttcaacctc ttctgcggta aaaccatctt
     8341 ttagaaccct tgtaaattca tcatctactg cttgttcaac acgatcacga ttctgaggtg
     8401 cgtagatggc gaagattttg aaatcgcctc gtgcttcaaa agcactcgcc gtgagcgaag
     8461 aaataacact gtatgaaaga ccctcttgtt cacgaacacg acgccacagc cgtgaatcag
     8521 ccgatcctcc gaataagtaa ttgcctagaa caagcgcagg aaaatcagga tgatcgtctc
     8581 gtattttgag attcatacca gcctgataaa ccgcattggc tttatctgga gtttccagat
     8641 tgagatcgat agccttgata tcttgatacg gtgtcgaaat acgttgatac ggcttagggc
     8701 ttttccagtc acctagcgat tgttgcaagg tctcggtaat agccttaggc tcaaaatcac
     8761 cgacaatcgc aagctctgca tccgtgcttc cataaaaatc ctgatgacag cgcttgacgt
     8821 catcgaggga tacttgattg atccgctcaa ttcgttcttc gattgtaggg gtatagagcc
     8881 aatgcgctga cgcgaaaggc attagatgac gctcgagttg gatacccgct tgcgcagcag
     8941 gttcactctt ttgattttca atagaggaca gaatcgctag cttgagctgt tcaaactctt
     9001 tctctgggaa aataggattg cgaaacattt ctgcgactaa ctgaagcgca tcggttaaat
     9061 gatcgccagt cgtttcaaaa gcaatgccat ccgctgtcat gtcgatgtgt acttttaagc
     9121 gatcaaaagc atcgcgcaat tgctcacgag tataacgctg tgtaccccgt gataacatcg
     9181 cgcttgtcat gccacaagca gtcgcacgat tcattttact ttgctcatct ccccaatgca
     9241 gattaaacgc gacgacaacg cgattgcctc gcgttttttt agggagtaag gcaagcttca
     9301 ttccgcctgg taattgcgtg cgaatgagtt ttgcttcgat attctcgggc gtggcttcaa
     9361 acgcttcccc caaactcagc attgcgtttc cacggtattc tttgaacatg agtgcgagat
     9421 cagtgaccgc tggaatttct gcacgatctg gtgtttttgt tggaatgaaa agacctaatg
     9481 ttcgattact gggtttaaaa taagtattcg ctacgcgctg tacatcttct aaggtaactt
     9541 gttggatccg atcacgatgt agaaacagca atcgccaatc tcccattgcg ataaattcag
     9601 tgatcgtcgt agccaaatta cgtgtgttgg tcagtaattg ctcgatttta ttaagtaggc
     9661 gtgtacgtgc gcgttccaat tcttcagcgg taatgggatg cgctgctaat gtttcgatgg
     9721 tactaatcag cgtattgctc gcatgatcga gtgacatagt cggtagcaaa ttagcgccga
     9781 aataaacaaa accggtttcg cgtaattggc gatcgaaacc aaaaatcgct gttgctaatt
     9841 tggattcgac gagcgattta tgcaaccgac cgccaggaac ttgtttgatg atggaaacaa
     9901 gcaaatcgat tgcagcagat tcaggatgtg cagcagccgg gatatggtaa agcgttgctg
     9961 caatttgtgt atcgccaacc cggcgtagca taacgttacg ttccccgtcc tgcgtcggtt
    10021 ctaccgtata ggtttttcgt agctggcgtt ttggatgagg aatagcgcca aagtgcttgg
    10081 atatactatt tagtgctgct gtttcatcga atttgccggc aatgacgagt actgcattat
    10141 cgggctggta ataatggtga taaaaatctt gtaatcgttc gatgggaaca ttttcgatat
    10201 ccgagcgaga accaatgatc gcgcggccat agttgtgcca tacgaatgcc gttgatgcca
    10261 tacgctcgga taacacacca aaaggattat tttcgcctgc ctcaaactca ttacgaacaa
    10321 ccgtcatttc gctatcaaga tccgcttttt caatacgaga gttgatcata cgatcggctt
    10381 ccagcgagat agcccaatct aagttttctg tactcgcagt caacacttcg taataattag
    10441 tacgatcata cgaagtcgtg ccattccacc gtgcgccccg cttggtgaat tcatttttga
    10501 tatcagggtg gcgttcggta cctttgaata gcaaatgttc aagcaaatgc gccatgccgg
    10561 cttcaccata accttcatcg cgtgaaccga ctaaataaac gatgtttacc gtcaccgtat
    10621 ctaaggaatc atcaggaagg agcaagactt tcaagccatt cggcatttga tactcggtaa
    10681 tgccttcaac tgatgtaatc tgagttaagt tagccggtaa tgactgcatg ggctgctgcg
    10741 catgccctaa gctcatgata ctcaggctaa taatgaaagt agtgatgatt aataagtaac
    10801 gccacatatc gtaagttgaa tcattaaaat tttaaagact aaatgttttg taggaagttc
    10861 tggttaatga ttaaaatgca aacggttacg caatcactaa tctttttcat cacgcgcgtt
    10921 tgaccattgc agttttaaat cgctcgcttt tttaaataga ctgagcagtg cctcaccatt
    10981 tcgattggca agttgttcac gtaactctgt tagcgcttgt tgatacttat cgagctcagc
    11041 taatagctgc acccggttcg ctaagcaaat gtcacgccac atctcggggg aactcgccgc
    11101 aatccgtgta caatcacgca aactactgcc agcataactc aaaacatagt taggatcgaa
    11161 atcagtacaa gtttgaatat gattcatcag tgtaaaagcc agcatatgcg gcaaatgact
    11221 cacgatcgct agcatttgat cgtgttgctc agcagacatg attgaaactt tcgcgccaca
    11281 tgactcccat aatactttga ccagattgat tgcttgttcg ttattttcct tcaaaggcgt
    11341 gatgatgagg tgcttctgtt taaataataa gggatcagca gcttcagcgc cgcgaaactc
    11401 cgttcctgca attgggtgag ctggaataaa atgggcaaga tgattggtca attgttgctg
    11461 cgcagcttcg atcacattct gtttggtact gcccacatcc gagactatcg tttgcttatt
    11521 caagtaaggc tcgatctgcg ccattactcg ctgaatttga ccaactgggg tagataacat
    11581 aacaaaatca gcttctgcca gcgccgcagc cggggtaatc gcgatctcat cgataatacc
    11641 caactcacgc gctcgtagca tattttcctg accgcgtccc aaaccaacaa tttgcttaac
    11701 aaggcctgca ttacgtaatg ctaatgcaaa cgatccacca atcaagccta cgccgataat
    11761 gacaagtttt tgaagatagg ggggggttgg catatcaaca agaatgaagg tattccatga
    11821 ctaccattta atgattaggc tgaattactg ataacctgtt ccaatgcact caagaatttc
    11881 tcattctctg attttaagcc gatcgtcaca cgtaagtgat gaggcatttc gtaaacgcct
    11941 aacggacgaa caatcacacc tcgtttcaac agattttgat aagctttagc tgtattaatt
    12001 tcatctcccc ggatacgtag cgtaatgaaa ttcccatagg aaggaatgaa ctccacacct
    12061 aattgccgca atccgttggt aatttcagcc ataccagcac gattgagttc ataagctcgt
    12121 cgaacaaatt ctgtatcttt taatgctgcc aacgcgccaa tcaatccgag actattgacg
    12181 ttaaagggtt gacgaatacg gttcattaaa tccgctaact cggggtgc
//
LOCUS       DAOQKQ010000073        12207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_2432_length_12207_cov_5.814845, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000073 DAOQKQ010000000
VERSION     DAOQKQ010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 12207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12207
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2432_length_12207_cov_5.814845"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="PKZ49_06320"
     CDS             <1..410
                     /locus_tag="PKZ49_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HND36213.1"
                     /translation="ILSREGFEAENLSDYKGLNQRNPWLALIMMFLMLSMAGIPPMIG
                     FYAKLSVLQAVVNAGFVWLAITAVVLSLIGAFYYLRIIKLMYFEAPEHDTPVLPSFDA
                     KILISVNGLAVLIAGIFPQALMGLSLQAIQTSM"
     gene            complement(420..1541)
                     /locus_tag="PKZ49_06325"
     CDS             complement(420..1541)
                     /locus_tag="PKZ49_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HND36214.1"
                     /translation="MMHKIFFIILPLLISSFTYAETSNSQQSTNLPLEKIKLPPGFSI
                     QLWATIPDAKSLAIGRQGTVFVSSKTTGSIYAISQPDGKEKQVKMIANGLKSPSGITY
                     YEGALYVTALNRVFRFDEIENNLDQINEPVIIYDNLPKESFHSTRYMAVGHDNLLYIS
                     VGAPCDACEADPMRYALIARLNPNGSNFEVYAQGIRNSLGFDWHPKTHELWFSDIGRD
                     WMGEDLPPDELNYAATQGLHFGFPNCHAGKILDPKYGAKRGCDKSQAPIAELEPHVSP
                     QGIKFYSGRMFPPEYHDQLIIAEHGSWNRRAPVGFRLQHFQLKNNQIVKKEIFAEGWF
                     NDQKVWGRPTDLLVMPDGALLVSDDLAGAIYRISYRITD"
     gene            1678..3603
                     /gene="htpG"
                     /locus_tag="PKZ49_06330"
     CDS             1678..3603
                     /gene="htpG"
                     /locus_tag="PKZ49_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HND36215.1"
                     /translation="MQASTQQKERMNFQAEAKQLLKLMIHSLYSNKEIFLRELISNAS
                     DAVDKLRFEGLTDSALYESDSDFKIRVSYDKDARTVTISDNGIGMSRQEVIDHIGTIA
                     KSGTREFFNSLTGDQAKDAHLIGQFGVGFYSAFIVADKVTLITRRAGLTPEHGVRWES
                     QGEGDYELETVEKRDRGTEITLHLREGEDDFLSGFQLKSIIRRYSDHITLPVMMKKEE
                     WVQESNEYKITDEDETINQASALWARSKNEITQEQYEAFYKHVAHDYEPPLAYVHAKV
                     EGKQEYTQLLYIPSRAPFDLYDREHRHGIKLYVKRVFIMDDAEKLLPNYLRFVRGIID
                     SSDLPLNVSREILQESKDIDNIRAGSVKKILSLIEDFAGSSKEEDQAKFRTFWKEFGQ
                     VLKEGVGEDYANRERIAKLIRFVTTSSDSDEQTVSLDDYIARMKEGQEKIYFVTADNL
                     KAARSSPHLEIFRKKGIEVLLLSDRIDEWLVSNLTEYAGKALQSVAKGSLDLGKLEDE
                     VEKQEQEKEAEAYKDLISRIKEVLGERIKDVRITYRLTESPACLVADMYDMSGNLERL
                     LKSAGQKVPITKPILEINPHHPMVQRLKYEEEKFSDWSNILFDQALLAEGGQLEDPAA
                     FVKRLNDLLLQTILIAK"
     gene            3687..4163
                     /locus_tag="PKZ49_06335"
     CDS             3687..4163
                     /locus_tag="PKZ49_06335"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HND36216.1"
                     /translation="MEDDKAYQAKLLTPFAVLGIRTEEDWLTGIDYLPRDIPEKIPQN
                     VLAKEVCLQLRAYLKDPQFTFDLLLHISGTHHQQRVWSSILEIPPGKTRSYAEIAKQI
                     HSAPRAVGQACGANRLPIVIPCHRVIAKNGGLGGFMHASDGMPLDIKRWLLRHESL"
     gene            4160..5092
                     /gene="xerD"
                     /locus_tag="PKZ49_06340"
     CDS             4160..5092
                     /gene="xerD"
                     /locus_tag="PKZ49_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HND36217.1"
                     /translation="MMLCDSDVKLIDEFIDSLWLEDGLSRNTLSSYRSDLKQLSLWKL
                     NNKSKQLSIAELDHSDLLAFLADRVAILIKASSANRELSTIKRFYQFLLRQRKIKIDP
                     SLNIDRPKLPRLLPVSLSETEVEILLSAPDILKSQGIRDRAMLELLYATGLRVSELVT
                     LQLLQVRVDMGVVKVLGKGNKERLIPFGEEALYWVNRYITEVRNSLLAGKNKITDKVF
                     ITNRGEAMTRQAFWYLIKRYAQHTGITKSLSPHQLRHAFATHLLNHGADLRVVQLLLG
                     HADISTTQIYTHIARERLKQLHARHHPRGSIFQQ"
     gene            5470..6834
                     /locus_tag="PKZ49_06345"
     CDS             5470..6834
                     /locus_tag="PKZ49_06345"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HND36218.1"
                     /translation="MGLKIAFSDKPFRKLWLIFAIISNLLLLGYFKYADFFISTVNYI
                     SSANVQTLSIILPIGISFYTFTQIAFLVDTYEGKVKEYRFIHYLLFVTYFPHLIAGPV
                     LHHKEMMPQFADSRIYRFSQSDFAVGITIFCIGLAKKVLIADNLAPYANNLFNEANSS
                     PSLLIAWGGVLAYTFQLYFDFSAYSDMAIGLSRLFGVNLPLNFNSPYKSRNISEFWRR
                     WHMTLSRFLRDYLYIPLGGNRVGLFKQYRNLMTVMLLGGLWHGAGWSFVIWGGLHGAY
                     LIIHHGWVKLSQKIQFPVTTMLWQIAATGLTFMSVCIAWVFFRSAELEDALEIIYGMF
                     GVFGIELPDAIGSRLIILAPFIQELGITFYLGGGKIFIETWSWIALAALIAFFFPNTQ
                     EIMQNFKPALDFQNSKIKSPTRNLLGYLVWQPSRWWSVWIGVITVISLLSLNRPNDFL
                     YFQF"
     gene            6843..8099
                     /locus_tag="PKZ49_06350"
     CDS             6843..8099
                     /locus_tag="PKZ49_06350"
                     /inference="COORDINATES: protein motif:HMM:NF019243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1574 family protein"
                     /protein_id="HND36219.1"
                     /translation="MEELQNSQRYLCWLLVITITGSLAVVIFVLIVDPYRQYGIVQYR
                     GFNIIKPTLERYQNEIKLSQVKRLKPDVLILGNSRAEIGFDPDAPVFNQKGLSAYNLS
                     IPGIGIETVYQQLLYLTKNGINPGLIFLGLDFLDFIDLPSQQEVNASIDPSQYLIDQQ
                     RKIESWFWRFDSMFSLASVKDAFRTLAIQNNEEAAIVTFHGFNPLNEYKPIVRAEGYY
                     KLFQQRAQENVRTYLKKAQGSVSPMSLNYLQAIFNLVEENNSKIILVIYPYHAQILAL
                     FEVTNLWPLFNQWKSLVVNELIDMNQYSAQIELYDYSGYSLYHCERIPPIGDLLSTTQ
                     WYWEAGHFKKELGDIILEEILRSNETILSKVMSMNHSQTSFGIRLLDQNSFLLNQNRI
                     IQQRLMCESNYPELFTDAAILARAAQ"
     gene            complement(8194..8892)
                     /locus_tag="PKZ49_06355"
     CDS             complement(8194..8892)
                     /locus_tag="PKZ49_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HND36220.1"
                     /translation="MATTLITGSNRGIGFEFARQYAQSGWNVIACCRQPENASNLRQL
                     ANSFPDLVTIYPLDVADHNQITNLSNNLRNTSIDLLINNAGIYPPSDDGGFGKIDYEA
                     WHKAFQINTMAPLRMAEAFLAQISNSQLKIIAIITSKMGSIDDNKRGGSYIYRSSKAA
                     VNMVGKSLSVDLKLQGITTVILHPGWVRTDMGGPDALISPEQSVLGMQRILSNLKLSD
                     SGKFFAYDGKIIPW"
     gene            9095..9964
                     /locus_tag="PKZ49_06360"
     CDS             9095..9964
                     /locus_tag="PKZ49_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44-like domain-containing protein"
                     /protein_id="HND36221.1"
                     /translation="MKKILTLFTLAVLTFTLLPVDAEAKRLGGSKSIGKQRDSINQQA
                     TPKPAQSQSAAPASNPAAGGSKWGGALAGLAAGGLLAALFMGGAFDGINMADILMLVV
                     LMAVIFFIVRMLRKPKSEQLSRPMQYSAAGAAQTNEVFSGGSSATSSSSTDKAYDGNA
                     TTNSIPPDFQVEPFLKNAKLSFINLQAANDAGDVDAIREYTTPELFEALNKQIQESGD
                     HPQKTEVIFINADLLDVTTSSDMAIASVRFNGQLREAPDALPEPFDEIWHVQKSLKEK
                     NAPWLLAGIQQPD"
     gene            10097..10837
                     /locus_tag="PKZ49_06365"
     CDS             10097..10837
                     /locus_tag="PKZ49_06365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HND36222.1"
                     /translation="MKIAVVSDIHGNLPALEAVVSHFNRIGIDKVINLGDSLSGPLLP
                     LETAHFLMAQNWIQLAGNHDHQILAHGTDQCSVVDELAYSQLSAKELDWLKSLPNTLY
                     LSQDILLCHGTPESSNSYLLETVVSSGARLATANEITERLGKDRKEKLILCGHTHQPR
                     SVKILGELLIVNPGSVGLPAYSDEIPFLHTIETGTPDAHYALLERVGNQWISSLISVP
                     YDYQAMVKLAESRHFMDWACALRTGFMN"
     gene            complement(11152..11940)
                     /locus_tag="PKZ49_06370"
     CDS             complement(11152..11940)
                     /locus_tag="PKZ49_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HND36223.1"
                     /translation="MQIITSREHTLFKQLIKLLRSTQQRKREKLTILDGIHLVQTYYQ
                     VYGSPFKLIISESACQQPETESFLKQFNQSSEPNRSVVLSDTLFKHISPVVTPSGIMA
                     LITVPIASHIHSKQFCVLLDAIQDPGNLGSILRSAASAGVNAVYLSKTCADAWSPKTL
                     RAAMGAHFFLTIHENAELVQIAGNFQGKVFTTSACSEHSLFQTKLTEPIAFVFGNEGS
                     GISGELMDCVSQSLAIPMNKNCESLNVAAAAAICFFEKLRQNLN"
BASE COUNT         3759 a         2083 c         2491 g         3874 t
ORIGIN      
        1 tgatcttatc ccgggaaggt tttgaggcag agaatcttag tgattataaa ggtcttaatc
       61 agagaaaccc gtggttggca ttgatcatga tgtttctaat gttatcgatg gcaggtattc
      121 ctcctatgat tggtttctac gcaaaactat ccgtgctgca ggcggttgta aatgctggtt
      181 tcgtatggct agccataaca gcagttgtgt tatcgctgat cggtgcgttc tattacttgc
      241 gtattattaa gcttatgtat tttgaagcac ctgaacacga cacacctgta ttaccttctt
      301 ttgatgcaaa aatactgatc agtgtaaatg ggttggcagt tttaattgcc ggtatttttc
      361 ctcaagcact tatgggtctt agccttcaag ccattcaaac ctcgatgtag catctcatct
      421 taatcagtta tcctgtaact gattcgataa atagcgcctg ctagatcgtc agagactaat
      481 agcgcgccat ctggcattac tagtaaatca gttggtcttc cccaaacctt ttggtcgtta
      541 aaccaacctt ctgcaaatat ttccttttta acgatctgat tattttttaa ttgaaagtgc
      601 tgtaaacgaa atccaacagg agctctacga ttccatgatc cgtgctctgc aatgatgagt
      661 tgatcgtggt attcaggagg aaacatacgc ccagaataaa atttaatgcc ctgagggctt
      721 acatgcggtt ctagttcagc aatgggtgct tgagacttat cacaaccccg ctttgcacca
      781 tatttaggat caagaatttt gccagcatga caattaggaa aaccaaaatg caaaccctga
      841 gttgcagcat agttgagctc atcgggtgga agatcttctc ccatccaatc tcttccaata
      901 tcagaaaacc atagttcatg ggttttggga tgccagtcaa agcctagcga attgcggata
      961 ccttgtgcat agacttcaaa attactccca ttaggattga gacgagcaat cagcgcgtag
     1021 cgcattggat ctgcttcaca ggcatcacaa ggagccccaa ctgaaatata taataagtta
     1081 tcatgaccaa cagccatgta ccttgtgcta tgaaaggatt ccttaggtag attgtcataa
     1141 ataattactg gttcattaat ttgatcaagg ttattttcga tttcatcaaa tcgaaaaact
     1201 cgattgagag cagttacata gagcgcacct tcataatagg ttattccgct gggagatttt
     1261 agaccatttg ctatcatttt gacctgtttt tctttaccat caggttgtga gatagcataa
     1321 atacttcctg tagttttaga gctaacaaat actgtgcctt gtctaccaat cgctaatgat
     1381 ttagcgtctg gtatcgttgc ccatagttgg attgagaagc cgggcggtaa ttttattttt
     1441 tctaaaggta gatttgttga ttgttgcgag tttgaagttt cagcataggt aaagctgcta
     1501 ataagcaggg gtaatataat aaaaaaaatt ttatgcatca tggaatatta tttagtttag
     1561 gcaaaattaa ctaagaaaaa gaataataaa atactagttt cttgatgtta ccgatttagc
     1621 acttgaattt tgatatttta atcatacaat cttaccgcgt ttttaaggag aaaatacatg
     1681 caagcatcaa cacaacagaa agaaagaatg aattttcagg ctgaagccaa gcaattactt
     1741 aaattaatga ttcattcttt atatagcaac aaagaaattt tcttaagaga attgatttca
     1801 aatgcatctg atgcagtaga taaactacgc tttgaagggc taactgattc ggccttatat
     1861 gaatcggact ccgattttaa aattcgagtg agctacgata aagatgctag aactgtgact
     1921 attagtgata acgggattgg catgtcgcgg caggaagtta ttgatcacat tggcacaatt
     1981 gcaaaatcag gaacgagaga gttttttaat tctttaacag gcgatcaagc caaagatgcg
     2041 cacttaattg gtcaatttgg agttggcttt tattctgctt ttattgtggc tgataaagta
     2101 accttaatta cgagaagagc aggactaact cctgaacatg gtgttcgttg ggaatctcaa
     2161 ggagaaggag attatgagtt agaaacagtt gagaagaggg atcggggtac agaaattact
     2221 ttacatttaa gagaaggtga agatgatttc ttaagtggtt ttcagctgaa atcaattatt
     2281 cgacgttatt cagaccatat cactttacct gtgatgatga agaaagaaga atgggtccaa
     2341 gaaagtaatg agtataaaat taccgatgaa gatgaaacta ttaatcaagc gagtgcactg
     2401 tgggctcgat cgaaaaatga aattactcaa gaacaatacg aagcgtttta taagcatgta
     2461 gcacatgatt atgagccacc gcttgcctat gtacatgcca aagtagaagg aaaacaagaa
     2521 tatacacagt tattatatat tccttcgcgt gcaccctttg atttatatga tcgcgaacat
     2581 cgtcatggga ttaaattata tgtgaagcgc gtatttatca tggatgatgc agaaaaatta
     2641 cttcctaatt atcttcgatt cgtccgtgga atcattgatt ctagcgattt acctttgaat
     2701 gtttctcgag aaattctgca agaatcaaaa gatatcgata acattcgagc tgggtcagtc
     2761 aaaaaaatac ttagcttaat cgaagatttt gctgggagca gtaaagagga agatcaagcc
     2821 aaatttagga cattctggaa agaatttggt caagttttaa aagaaggagt aggtgaggat
     2881 tacgcaaacc gggagcgtat tgctaagtta attcgatttg taacgacgag tagcgatagt
     2941 gatgaacaaa ccgtttcatt ggatgattac attgctcgca tgaaagaagg tcaagaaaaa
     3001 atatattttg taacagcaga taatttaaag gctgcgagaa gcagtcctca tcttgaaatt
     3061 ttccgtaaga aaggaataga ggttttattg ttatctgatc ggatcgatga atggttagtc
     3121 tcaaatttaa ctgagtatgc tggtaaagct ttacagtctg tggcgaaagg aagccttgat
     3181 ctcggtaagc ttgaagatga agtcgagaaa caagagcagg aaaaagaagc ggaagcctat
     3241 aaagatttaa tttcaagaat aaaagaagta ttgggagaga ggattaaaga cgtacgtatc
     3301 acttatcgtt tgacagaatc gccggcttgc ttggtagcag atatgtatga tatgagtggt
     3361 aatctcgaaa gattactcaa atctgcaggt cagaaagttc ccattaccaa accaatttta
     3421 gaaattaacc cgcatcatcc aatggttcag cgcttgaaat atgaggaaga aaaattctcg
     3481 gattggagca atattctgtt tgatcaggca ttacttgcag aaggaggaca attagaggat
     3541 ccggctgctt ttgtaaaacg cttaaacgat ttacttttgc aaactatttt gattgcaaag
     3601 taaatgagag taacgcttca taatcagtaa attagtttgc ttatagcgag gaagtagcat
     3661 tgggaagtga taagtcaagt gtgaaaattg aagatgacaa agcttatcaa gctaaattac
     3721 ttacaccttt tgcagtgcta ggcataagaa cagaagagga ttggttaacg gggattgatt
     3781 atctaccgag agatataccc gaaaaaatac cacagaacgt attggctaaa gaggtatgcc
     3841 tacaattgag ggcgtacctc aaggaccctc aatttacgtt tgatttatta ttacatatta
     3901 gtgggactca ccatcaacaa agagtctggt ctagtattct cgagattcca cccggaaaaa
     3961 cgagaagcta cgcagaaatt gcaaaacaaa tccactcggc accacgtgcc gttggtcaag
     4021 catgtggtgc caaccgtttg cctattgtga ttccctgtca tcgagtcatt gcgaagaatg
     4081 gtggattagg tggctttatg catgccagtg atggtatgcc gctcgatatt aaacgctggc
     4141 tgctcaggca tgaaagctta tgatgctatg tgatagtgat gttaagctca ttgatgaatt
     4201 cattgattca ttatggttag aagatggatt atcgaggaat acactctcaa gttaccgcag
     4261 tgatctaaaa caactttcgt tatggaaact taacaataag agcaaacagc tatcgatagc
     4321 tgaacttgac cactcagatt tacttgcttt tctcgctgat cgagtcgcaa ttttaataaa
     4381 agcaagttct gcaaatcgag aattatcgac gataaaacgg ttttatcagt ttttattacg
     4441 tcagcgaaaa atcaagatcg atcctagttt aaatattgat cgaccaaaac taccgcgatt
     4501 gcttccagta agtttatctg aaactgaagt agaaatactt ttgagtgcac ctgatatttt
     4561 aaaatcacaa ggaattcgcg atcgagctat gctagagctg ctctatgcca cgggtttacg
     4621 tgtttcagag ttggtgacac ttcaattact gcaggttaga gttgacatgg gcgttgtgaa
     4681 agtactaggt aagggtaata aagaacgatt gataccgttc ggtgaagaag cactttattg
     4741 ggttaatcgc tatataaccg aggttcgtaa cagcttattg gcaggaaaga ataagataac
     4801 cgataaagtt tttattacca atcgaggcga ggcgatgact cgccaagcat tttggtattt
     4861 gattaaacgc tacgctcagc atacaggtat tactaaatca ctttctccac atcagttacg
     4921 gcatgctttt gcaacccatc tcctgaatca cggtgctgat ttaagagttg ttcaactgct
     4981 acttgggcat gctgatattt ctacaactca aatctatacc catattgctc gtgagcgtct
     5041 gaaacaatta catgcgcgcc accatccaag aggttctatt tttcaacagt aaattgattt
     5101 tataaagcgc caattttgtt cattttttaa taattgaaat attgctagaa gcagtatgat
     5161 ggttattaat tctttctaaa ttagcttgga ataggatagg ttatctttaa tagaacagtt
     5221 gattggagaa aaatgaagaa tggaatgatg tttgtggaaa aataaggcca attattctta
     5281 atgctattta attcttttac ttttatattt ttttatttgc ccattaccgt tgtgggtttc
     5341 ttttggctga gccgctatag ttatacttta tcagctgggt ggttagcatc tgcttcaata
     5401 gttttttatg ggtattggga ctatcactac ataccacttc ttctaagttc aatctgcttt
     5461 aatttttgga tggggctcaa gattgcattc tcagataaac cattccggaa gctttggctt
     5521 atttttgcga ttatctcaaa tctgttatta ctcgggtatt ttaagtacgc agattttttt
     5581 atttctacag ttaattacat tagtagcgca aatgttcaaa ctttgagcat tatcttgcct
     5641 attggcattt ctttttatac atttactcag attgcattcc tcgtggatac atatgaggga
     5701 aaagttaaag aatatcgctt cattcattat ttactatttg ttacttattt tcctcatctt
     5761 attgctggac cagtattgca tcacaaagaa atgatgcctc aatttgctga ttcgagaata
     5821 tatcgttttt ctcaatctga ttttgcagta ggaataacaa tattttgtat tggtttggca
     5881 aagaaagttt tgattgcaga taaccttgct ccctacgcaa ataatttatt taatgaagcg
     5941 aactcttccc catcattact aatagcatgg ggaggtgtat tagcttatac gttccaactc
     6001 tattttgatt tctcagctta ttcagatatg gcaattggtt tatcgcgact atttggagtg
     6061 aatcttccac taaattttaa ttcaccctat aaatcaagaa atattagcga attttggcga
     6121 aggtggcaca tgactctgtc acgctttctt agagattatc tatatattcc acttggtggt
     6181 aacagggtag ggttattcaa gcagtatcga aatttaatga ctgttatgtt attaggtggt
     6241 ttatggcatg gagctgggtg gagttttgtt atatggggtg gacttcatgg agcttatcta
     6301 attattcacc atggatgggt caagctttca caaaaaatac aatttccagt aacaacaatg
     6361 ttatggcaga tcgctgctac tggtctaaca tttatgtcag tttgcattgc ctgggttttt
     6421 tttcgttcag cagagctgga agatgcatta gaaattattt atgggatgtt tggagttttt
     6481 ggtatagaac tacctgatgc aattggtagt cgacttataa tattggcccc attcattcaa
     6541 gaattaggca taacatttta tctaggaggg gggaaaattt ttattgagac ttggtcttgg
     6601 attgctttag ctgcacttat tgctttcttt ttccctaaca cacaagaaat tatgcaaaat
     6661 tttaaacctg ctctagattt tcaaaactcg aaaattaaaa gtccaactcg aaatttgcta
     6721 ggttatttag tttggcaacc atcacgctgg tggtcagtat ggatcggggt aattaccgtg
     6781 ataagcctat tgtcattaaa tcgcccgaat gattttttgt attttcagtt ttaattcaga
     6841 gtatggaaga attacagaat tcgcagcgat atctttgctg gctattagta attacgatta
     6901 ctggtagcct agcggtagta atttttgtac ttatcgttga tccttatcgc cagtatggca
     6961 ttgttcaata tcgtggattt aatattatta aacccacatt agagcgctat cagaacgaga
     7021 ttaaattatc ccaagtaaag cgactgaagc ctgatgtact tattttaggt aattctcgcg
     7081 ctgagatcgg atttgatcct gatgcaccag tgtttaatca aaaagggcta tcagcctata
     7141 atttaagtat accggggata ggaattgaaa ctgtatatca acagcttctt tatttgacaa
     7201 aaaatggtat taatccgggg ttgatttttc ttggccttga ttttttagat tttattgatt
     7261 taccgtctca gcaggaagtc aatgcatcaa ttgatccttc tcagtattta atcgatcaac
     7321 aaagaaaaat tgaaagttgg ttctggcgtt ttgatagcat gttttctcta gcttccgtta
     7381 aggatgcctt tcgtacactt gctattcaaa acaatgaaga agctgcaatc gttacttttc
     7441 atgggtttaa tccacttaat gaatataaac caattgtacg agctgaaggg tattacaaat
     7501 tatttcaaca aagagcacaa gaaaatgtta gaacttatct taaaaaagcg caaggctcag
     7561 tgtcgccaat gagtttgaat tacttacagg ctatatttaa tctagtagag gagaataata
     7621 gcaaaattat tcttgtgatt tacccctatc atgcccagat actagcactt tttgaagtga
     7681 caaatttatg gccattattt aatcaatgga agtctttagt agtcaatgag cttattgata
     7741 tgaatcaata ctcagcgcag atagaattat acgattacag tggttatagt ctctatcact
     7801 gtgagcggat tccgccaata ggcgatctct tatctactac tcaatggtac tgggaagcag
     7861 gtcatttcaa gaaagaactt ggggatatta ttttagaaga aattctgaga agcaatgaaa
     7921 cgattctctc aaaagttatg agtatgaatc attcacagac atcatttggg ataagattac
     7981 ttgatcagaa tagttttcta ttgaatcaaa atcgtattat tcaacaacgt ttaatgtgtg
     8041 agagtaatta ccccgagtta tttactgatg ctgcgatatt agcaagggca gcacagtaaa
     8101 ttatgtatac aattttgcaa agtattccaa actaacttta accggttgtg ccttgtacag
     8161 aattgttgag cctataagtg tttcctcaag tctttaccaa ggaataattt tcccatcata
     8221 ggcaaaaaat tttccagagt cggataattt tagattcgat aatatacgct gcataccgag
     8281 tacactttgt tcagggctaa ttaatgcatc tgggccgccc atatcagttc taacccatcc
     8341 aggatgcaga atcacggtag taattccctg tagttttaag tcaactgaaa gacttttacc
     8401 aaccatattg acagctgctt tgcttgatcg gtaaatataa cttcctcccc ttttattatc
     8461 atcaatactg cccattttac tcgtgatgat cgctatgatt tttaattgac tattacttat
     8521 ttgtgcaagg aatgcttctg ccattcttaa tggtgccata gtattgattt gaaatgcttt
     8581 gtgccatgct tcgtaatcaa tcttaccaaa gccaccatcg tcagatggtg ggtagatacc
     8641 tgcattattg ataagcagat caatggaagt atttcttaaa ttgttggata aatttgtaat
     8701 ttgattgtga tcagcgacat ccagcggata tatcgttacg agatcaggaa agctattggc
     8761 aagttgccta agattagaag cattttcggg ttgacggcaa cacgcgatga cattccatcc
     8821 gctctgcgca tattgccgtg caaattcaaa tcctatgcca cggttacttc cagtgattaa
     8881 agtagttgcc ataaataaaa tcaatccctt tgtttttttg ctattagagt ttagtgtttt
     8941 attgagttat tttaagagta tttcataaat tggttagaaa taaccaacat taaaattcta
     9001 attgctacta gcattcccaa aaaattccaa tgagaaacaa gaataaatcg atgatgttat
     9061 actttctaac tcaacaatgt ttatggagaa aaaaatgaag aaaatactta ctttatttac
     9121 attagcggtg cttactttta ctttattgcc agttgatgct gaagcgaagc gcttaggcgg
     9181 tagtaaaagt attggaaagc agcgcgattc aattaatcag caagcaacgc ctaaacctgc
     9241 tcagtcacaa tcagcagcac ctgcttctaa tccagcggct ggtggaagta agtggggtgg
     9301 ggcattagca ggtttagccg ctggtgggct actcgctgca ttatttatgg gtggtgcatt
     9361 tgatggcatt aatatggctg atattcttat gctggttgta ttaatggcag ttatattctt
     9421 tattgtacgc atgctacgta aaccaaaatc tgagcagcta tcgcgtccta tgcagtattc
     9481 agcagctggc gcagcgcaaa ccaatgaagt attttctgga ggctcatcag caacctcgtc
     9541 aagctctacc gataaggctt atgacggtaa tgctacaact aattcgatac caccagattt
     9601 tcaggtagag ccgttcttaa aaaatgcaaa attatcattt atcaatttac aagcagcaaa
     9661 tgatgcgggt gatgttgacg ctattcgtga atatacaact ccggaattat ttgaagcatt
     9721 gaataagcaa atacaagaaa gtggtgatca cccacaaaaa acggaagtga tctttattaa
     9781 tgcggacttg ttggatgtta cgacgtcaag tgatatggcc attgcaagcg tccgttttaa
     9841 cggtcaatta agagaagcac ctgatgcatt accagaacct tttgatgaaa tctggcatgt
     9901 acagaaaagc cttaaggaaa agaacgcacc ttggttactc gccggtattc aacaacctga
     9961 ttaatgtagt aaggaatcga taaatcaatt accctgtttc atttttttga aaggcgaggc
    10021 agggtaattc gattgtactt ctattttctc gtgattctag caaagataaa tttcttttct
    10081 atgtagttgt tatctaatga agattgcggt tgtttcagat attcacggca atttacccgc
    10141 actagaagca gtcgtttcac atttcaatcg tataggtatt gataaggtaa ttaaccttgg
    10201 cgatagctta tctggtcctt tattaccact agaaacagca cattttctga tggcccagaa
    10261 ttggatacag cttgcaggta atcatgatca tcagatatta gcgcatggga ctgatcaatg
    10321 cagtgttgta gatgaattag cctattctca gctttcagct aaagaattag actggctgaa
    10381 gagtttaccc aatacactgt acttgagtca ggatatttta ctttgccacg gtacacctga
    10441 gagtagtaat agttaccttt tggagactgt tgtgtcgagt ggagcgcgcc tagcgactgc
    10501 aaatgaaatt acagaacggc taggaaaaga tagaaaagaa aaattgattt tatgtgggca
    10561 tacgcatcag ccacgttcgg taaaaatttt gggtgaatta ctcattgtta acccagggag
    10621 tgttgggtta cctgcttata gtgatgagat accgtttctt catacgattg aaacaggtac
    10681 ccctgatgca cattatgcgt tactagaaag agtaggaaat caatggatat cttccttaat
    10741 atcggttcct tatgattatc aagctatggt aaaactggct gaatcacgcc actttatgga
    10801 ctgggcgtgt gcattaagaa ctggtttcat gaattaagaa taaatttctc agtagtagaa
    10861 tattgccatt actagaaaat atgcgtagaa tcctagccag ccctattgtg gttatgggtt
    10921 tataaaacta aagaatagat tttcataagg agataagtgt tatggaaaat aatagagcga
    10981 cagaagctag tacgttcaaa gtagtagcta tttctacgat tgggttgatc gttattggtg
    11041 cagttgttta ttttgtaact gcagtctaat agtggcgtta cgttagttca attggtttac
    11101 actcatactc agtaatgagt atgagtggtt gtaatatcaa atagtcgtgc atcaatttaa
    11161 gttttggcga agtttctcaa aaaagcatat agcagcagcg gcagcaacat tcaaagattc
    11221 acagttctta ttcatcggga tagcgagtga ttgactaacg caatccatta attctcctga
    11281 aataccagat ccttcattac caaatacaaa agcaatcggt tcagtaagtt ttgtttggaa
    11341 caagctatgt tcagaacacg ctgaagtggt aaatactttt ccttgaaaat tgcccgctat
    11401 ctgaactaac tcagcattct catgaatggt taagaagaaa tgagccccca ttgccgcacg
    11461 taatgttttg ggagaccagg cgtctgcaca agtttttgat aaataaaccg catttacacc
    11521 agcgcttgct gcagaacgca gtattgatcc taagtttcct ggatcttgta ttgcatcaag
    11581 taatacacag aattgcttgg aatgaatgtg gctagctatc ggtactgtaa taagagccat
    11641 aatccctgag ggagttacaa caggggatat atgtttgaat aaggtatcgc tcaaaacaac
    11701 agagcgatta ggctcagatg attggttaaa ttgctttaga aaagattctg tttcaggttg
    11761 ttgacaagca gattcactga taatgagctt gaatggtgat ccataaacct gatagtaagt
    11821 ttgaacaaga tgtattccat ccaaaatggt aagtttttct cgctttcgtt gttgagtcga
    11881 acgcagtagc tttattagct gtttgaataa agtgtgttca cgggaagtga tgatctgcat
    11941 aaaagataat ttgtaagatg tatgaattta agcgttgcaa tgtatttagg aaaaaactgt
    12001 gattggattt gggtgataaa aggtaacaca gtaggcacct caaagtcggc tgattacgat
    12061 ttcaattttt aactccagga ttacttcaaa taagactata taaactagca gcttaaacac
    12121 gatagtaata ttgtttcaag tgtttagtgc gtaaatctga ataaagactt gcctgaataa
    12181 tgaattggct tgtattatct actcata
//
LOCUS       DAOQKQ010000074        12070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_2461_length_12070_cov_6.874906, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000074 DAOQKQ010000000
VERSION     DAOQKQ010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 12070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12070
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2461_length_12070_cov_6.874906"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..455)
                     /gene="rplS"
                     /locus_tag="PKZ49_06375"
     CDS             complement(69..455)
                     /gene="rplS"
                     /locus_tag="PKZ49_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HND36224.1"
                     /translation="MNLIKQFEQEEIERLGKVIPNFNPGDTVQVNVNVVEGDRKRVQA
                     YEGVVIAKRNRGLNSSFIVRKISSGEGVERTFQTYSPMIASIEIKRRGDVRRAKLYYL
                     RNLSGKAARIREKLTVRTEAVVDDKK"
     gene            complement(485..1258)
                     /gene="trmD"
                     /locus_tag="PKZ49_06380"
     CDS             complement(485..1258)
                     /gene="trmD"
                     /locus_tag="PKZ49_06380"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HND36225.1"
                     /translation="MSLEIDVVTLFPEMFDAVTKYGVTGRAEKNKVYQLNTWNPRDYA
                     EDSRKSVDDSPYGGGQGMVLLAAPLEKAIVNARARLKSIGIDDAHVIYLSPQGKLLDH
                     EKVLELSGYGSLILVCGRYEGVDERFITRYVDEEISIGNYVISGGELAAMVLIDSIVR
                     HLPGSLGDNNSAKEDTFSNLLLEFPQYTRPEIYNGEPVPSVLLSGNHAKIKRWRLQQS
                     LGKTWLNRPDLLAQKCEEGLSKEEELLLEEFKQQQDYKS"
     gene            complement(1271..1783)
                     /gene="rimM"
                     /locus_tag="PKZ49_06385"
     CDS             complement(1271..1783)
                     /gene="rimM"
                     /locus_tag="PKZ49_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964743.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HND36226.1"
                     /translation="MGHIIGPHGILGCVKVACYTEKITALLDYPTWWIGSDSKADIWH
                     EIGVKSYFVHADRLVVLLENITDRTEAIKLKGAQIAIPKSQLPVLPENGDAGYYWVDL
                     IGTQVVNIQNEVLGRVVGLLETGANDVLRVKDDTKDSKERLVPFIDHVVIQVDLKSKK
                     ILVDWGLDYC"
     gene            complement(1828..2085)
                     /gene="rpsP"
                     /locus_tag="PKZ49_06390"
     CDS             complement(1828..2085)
                     /gene="rpsP"
                     /locus_tag="PKZ49_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HND36227.1"
                     /translation="MVVIRLARGGAKKKPFFSMVVADSRFRRDGRFIERVGFYNPSAK
                     GGEEKLRIQLERINHWKSHGAQLSDTVTRLVKQFGSQQQAG"
     gene            2284..2565
                     /locus_tag="PKZ49_06395"
     CDS             2284..2565
                     /locus_tag="PKZ49_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36228.1"
                     /translation="MFKKSLSLIALLAALFFSINSFAATACSADTEKDGKTACSANEA
                     CYLVEGTCIEKKSLPEGKACKKSGVCTNLCVKDDGTETKSDGSETGKCN"
     gene            2777..2968
                     /locus_tag="PKZ49_06400"
     CDS             2777..2968
                     /locus_tag="PKZ49_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfB family protein"
                     /protein_id="HND36229.1"
                     /translation="MDVSAIAEVATTLSEARTTQAVGIAVLKKAIDQNAENALSLISA
                     LPNLQNLPPHLGKNINVVA"
     gene            complement(3032..8140)
                     /locus_tag="PKZ49_06405"
     CDS             complement(3032..8140)
                     /locus_tag="PKZ49_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HND36230.1"
                     /translation="MIAKAKINITSVVGIREAIEQIFVLIRKHIDAYAQNTEDRMQIE
                     SCQYYVHQLNGMLEMLELEGVLFVSQKMEGLIEALLQEKVESPSQTRVVLKQATRAIY
                     RYLDALIDGISDNPATLLPIYRKLMQVQGIKEISESDLFFPDLRQDLPLPKTIPEGDP
                     SQKLSTVKKVRSEFQTGLLKWLKDTSNKEGLKQMFDAVNLAEQIPAPIEQRTFWWLSS
                     GFLDGLIHQDESIDLSTRRLCGKIEQEIRHFDKDAHVVANQLMREILYRVARSSSNSE
                     RIKEIENVFDWRASLSSFDQLANAVAGPDEDSIQPILDAMHEILLGANDQWREYSAGN
                     QESLEVLMTAIDKLKQLAPQVNCAPLEKLISVMSGAVAFLRIRPQNMGDRLAIDLASS
                     LLIVENAIENFFQLSPEFPDQVEALAARIRADTSRKDDEIVLPDLPGPDETGYQVQEK
                     KLFKQIAQESLISLAQIEDILDRFFYDPSIRVDLPVLPDLFKQIAGVFSMLELDRANV
                     LLTLCRDLVTKLLDPEYQISQTEQTLLADGLSSLSFYIEALKNSQFDRDQIVESAINL
                     FGQSIVPNIEVPNIAEQVTFELVEPQSSAGLEPVTVVMAQTGDSELLAVFLEESDDVL
                     SSIAEKLSACRSNPADLEALAAIRRGFHTLKGSGRMVKLYDLSEAAWRIEQVMNRWLS
                     ERNPATEELLDLLECGRTTIGIWCDSLKKIGTAEIDPTELFELARRLMYASGPEKGGI
                     GEIETEVRRDVATEQIEPLPLATEVQTSIAVGNIKIPHDLFFVFTSEAGKHAETLNAE
                     LSDLTENPNAPIKHEFMLAAHTLSSISRTLGLTYIADIGVILEQYLTQLLRKSGRPDE
                     SALALVKETVSILDSMVSSVRNQQQPSDENIETGKRLSSELSTLLEKLQAEETEVADI
                     HYEQDLVDQAISTEQRAFVSSGLRDVSISDQDKIDLELLEVFIEEAHELLPEIGRNLR
                     LWRAKFEDYSAREGLLRALHTLKGSARIAGAMALGELLHQMENTVEQTSRENLSVTLF
                     DRLEAEFDEINDHIEQLQRSHTAEAQFAPSIIPVTTTAEVPAQSAIRSFNEHVIEAPQ
                     SEPIDSEFTTAKTVLRVDSALIDRLVNESGEASIMRSRVDAELYGFKQSLQDLDESTE
                     RMRGQLREIEIMAETRMPSGVITASDNQSAFDPLELDRFTRFQELTRLMAESLDDIVT
                     VHKSLREIHRVTLDAVGQQARLNSQVQHELIHLRTVPFKHYSEHFYRVVRQVSRDVGK
                     RVNLTIHGDEIEIDRSVLDKINSPLEHLLRNAIVHGIESTDKRLQLGKPEEGQITIDL
                     SRDGNEIILILHDDGAGLDFALIREKAKQLGIADEDTITDEQVKSVLFTHGFTTLQAV
                     NEVAGRGVGLDVVKNEITDLGGQIEVTSTLNQGTSFTLRLPITLALAQAVLVKVGEHT
                     YAVPSAQVVHILELNNEGLAAAYQQQSINFQGHCYSLAYLPNLLNITNQSPEIKRHNR
                     ILLLQNGDLFLAVHVDTLIGNCEIVVKNSGLQLSQVPGVEGATILGDGSIVLIVNLIK
                     IFQRKGEQALILDSPLERFDLNKQIEKPKLPIVMVVDDSLTVRKVTSRLLEREGYEVI
                     IAKDGVGALQILQEILPSIMLVDIEMPHMDGFELIRAVRNNVEMSKIPIIIISSRTAE
                     KHRKLAAELGVHVFLGKPYQEEELLEHIIKLIHK"
     gene            complement(8233..10233)
                     /locus_tag="PKZ49_06410"
     CDS             complement(8233..10233)
                     /locus_tag="PKZ49_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HND36231.1"
                     /translation="MAQNRFWLYGGIFLIFVFIALATMFVSLRYTKQSASYLEMIGRM
                     QIYNQNLTNASQQALLGNEIAFVQLQDSKNYLNQFSELLRGENKYPETLPTSMHVALS
                     DELLDEYQRVWRIKENKIGLLINSRDVLINVAGIANDINIASIRLQELLYELSVQMEQ
                     LGGLSKEVMITKLIGMLARSLTKSVNTLLSGEFVISDITEQLTKEREQLLVLLKALSD
                     GDSDLGIPALKDKRVLEKLVPIQALSKAIDDQVRIIQKQAPEVIAAKLALHDSFKINE
                     VLLSTLDKLKSKIEEQEASAKTWQDLVFYLSIGLVLGSLIIFGRTFSDYLQRQKAVGE
                     REVESAQKAILRLLDEMGALSEGNLTVRAAVTEDITGAIADSINYTVEELQKLVVQIN
                     QAGTNVNEVSSQAQRVSTQLLAATHGQSRQIEEATVSVLSMAESINTVASAAEEGANV
                     AKHSLTAAEKGAIAVRDAIMGMNEIRTSIQETAKRIKRLGESSQEIGEIIALISDITE
                     QTNVLALNAAIQAASAGEAGKGFSVIAQEVQRLAEHSAESTKQISSLVRAIQGDTKET
                     IAAMERSTYGVVEGTKRANATGEALEEIEEIANRLAQLVAKIFDATQIQTKSVDKVTK
                     NMENILAITRQTNDGTSQNAKLVKQISGFASELKASVAKFKV"
     gene            complement(10572..>12070)
                     /gene="ilvA"
                     /locus_tag="PKZ49_06415"
     CDS             complement(10572..>12070)
                     /gene="ilvA"
                     /locus_tag="PKZ49_06415"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HND36232.1"
                     /translation="LERILTARVYDVATETPLEKAANLSARIHNQVFLKREDTQQVFS
                     FKLRGAYNKMAKLSPTALKRGVIAASAGNHAQGVALAAQKLGSSATIVMPATTPQIKI
                     NAVMAMGATVLLHGDSYNDAYEHAMTIAQQKETTFIHPYDDPDVIAGQGTIGMEILRQ
                     HGAGIHAIFVPIGGGGLIAGIAAYVKRLYPNIKIVGVEPVDSDAMYRSLQKGRRVNLS
                     QVGLFADGVAVRHVGKETFRLCREFVDEVILVDSDSICAAIKDVFEDTRAILEPSGAL
                     SVAGLKHYVAREKLLHRSLVAVASGANMNFDRLRYVSERAELGEQREAVLAVSIPEEP
                     GSFKKFCSMLGTRSITEFNYRYADSKQAHVFVGVSVQNREEVVKLIKSLEANGLRTED
                     LSGNEMAKLHVRHMVGGRSKEVKHEILYRFEFPDRPGALMKFLNNMSHHWNISLFHYR
                     NHGADFGRVLVGIQVPLSEKVDFQKFLDNLGYRYWNESHNPAYKLFLL"
BASE COUNT         3476 a         2850 c         2117 g         3627 t
ORIGIN      
        1 attcaatcag atagaaaatg aaactcacgc tagtattcaa cttctctaat ttctagattg
       61 gagaaagatt actttttatc gtctacaact gcttccgtgc gcaccgtaag tttttctcga
      121 attctggcgg ctttacctga taggtttctt aaataataca attttgcgcg acggacatcc
      181 ccacgtcgtt ttatttcaat actcgcaatc attggggaat aagtttgaaa tgttcgctct
      241 actccctcac cgctagatat ttttcgaaca atgaaagaag aattcaaacc acgattacgt
      301 tttgcaatca caaccccttc atacgcttga acacgttttc gatcgccctc tactacgttc
      361 acatttactt gtactgtatc gccaggatta aaattaggta ttactttacc taatcgttca
      421 atctcttctt gctcaaattg tttaatcaga ttcatacaca ctccctatac acttattttc
      481 tattctaaga tttataatct tgttgctgtt taaattcttc gagtaaaagc tcttcttctt
      541 ttgagagccc ctcttcacat ttctgagcta ataaatcagg tcgattcaac caagttttgc
      601 ctaatgactg ctgcagtctc cagcgcttaa tttttgcatg atttcccgat aataatacgc
      661 ttggtaccgg ctctccatta taaatctcag gccgagtata ctgaggaaat tcaagcaata
      721 gattcgaaaa agtatcttct tttgcagaat tattatctcc aagcgaacct ggaagatgtc
      781 gcactatact atcaattagt accatggcag ctaattcacc gcctgaaata acataattac
      841 caattgatat ttcctcgtct acatatcggg tgatgaagcg ctcatctacc ccttcatatc
      901 gcccacatac taaaatcaaa ctaccataac cgctcaactc tagaactttc tcatgatcta
      961 gcaacttacc ttgtggtgac agataaatta catgggcatc atcaattcca attgacttaa
     1021 gtctagctct cgcattaact attgccttct ccagtggcgc tgctaacagc accattccct
     1081 ggccaccacc ataggggcta tcatcaactg atttccggct atcttctgca taatcacgag
     1141 gattccaggt atttaattga taaactttat tcttttctgc tctccctgtt actccatatt
     1201 ttgtaacagc atcgaacatt tctggaaaaa gtgtaactac atcaatctcg agtgacaaga
     1261 cttgctcctt ttagcaatag tccagccccc agtcaaccaa aatcttttta cttttaagat
     1321 caacctgaat tacaacgtga tcaatgaaag gtactaacct ttctttgcta tccttagtat
     1381 catctttgac gcgtaataca tcattagcac ctgtttccag taatcccact acccgcccta
     1441 atacttcatt ctgaatatta actacctgag tcccaattaa atctacccaa taataaccag
     1501 catctccatt ttccggtaga acaggaagct gactcttggg aatcgcaatt tgtgctcctt
     1561 ttagcttaat agcttctgtt cgatcagtaa tattttccag caaaacaact agcctatcag
     1621 catgaacgaa ataggattta acgcctattt catgccaaat gtctgcttta ctgtcactac
     1681 ctatccacca ggttggataa tcaagtagtg cggttatttt ttcagtgtaa caggccacct
     1741 taacacaacc aagaattcca tgaggcccaa taatatgccc cattggaact aactgttcat
     1801 ctagtgtctc gatagaagtt ttctcactca acctgcttgc tgttgactac caaattgttt
     1861 cactaatcgc gtgactgtat cggacaactg agctccatgc gatttccaat ggttaattct
     1921 ttccaattgg atacgcaatt tttcttcccc accttttgca gaggggttat aaaaaccaac
     1981 ccgctcaata aaccgaccat ctcgtctaaa tcgtgaatca gctaccacca tactgaagaa
     2041 gggctttttc ttagcgcccc cacgtgctaa tcgaataact accatataat cttatccaac
     2101 gttaaaaatc cttaggaggc agtattttag gataacctca tagtatttag caagcgctat
     2161 aatcttatca cttagttgat tttgctatag attgaacagc ttgatctttc gcgcactagg
     2221 ccagataatt aacaaacaca aactaaattt tgtttgttaa taaaatacaa aggaggaaga
     2281 atcatgttca aaaaatcttt aagcttgatt gcattacttg cagctttatt tttttcgatt
     2341 aattcatttg ctgcgactgc ttgtagtgct gatacagaaa aagatggtaa aaccgcatgt
     2401 agtgcgaacg aagcttgcta tctagttgaa ggtacttgca tcgagaagaa aagtcttcca
     2461 gaaggaaaag cttgcaagaa atccggtgtt tgcacaaact tgtgcgtaaa agatgacgga
     2521 actgaaacca aaagcgacgg ttctgaaaca gggaaatgca attaaacaat tttccgttta
     2581 attgatggat tgggggttag tagtgataca aagtatccta ctacccagac taaaacgcac
     2641 tgccaaactg ggattgatca gataattcaa gtttacggat tcctcctcat caagattcag
     2701 gctctaataa atacagctaa acatcctgca ctgagatccg attaaaaaaa tacggactag
     2761 aacaaaggag caaattatgg atgtctcagc cattgcagaa gtagcaacca ctttgagtga
     2821 agctagaact acccaagcag taggaatagc ggttctaaaa aaagcaatcg atcagaatgc
     2881 agaaaacgca cttagcctaa tctcagcatt accaaactta caaaatctcc caccccatct
     2941 tgggaagaat atcaacgtag ttgcctaaac agcggtaata gctaaatttt attaatttta
     3001 gtctaataac atttagcttt tacctaatgc ttcatttatg gataagtttg ataatatgtt
     3061 caagtaactc ttcttcttgg taaggttttc ccaagaatac atgcacacca agttctgcgg
     3121 ctaattttcg gtgcttctca gctgttcgag aagaaataat aataataggt atttttgaca
     3181 tctccacgtt attacgaact gctcgaatta attcaaatcc atccatatgt ggcatttcaa
     3241 tatcaaccag cattatcgat ggaagtattt cctgcagaat ctgtaaagca ccaacaccat
     3301 ctttagctat tatcacctcg tacccttcac gttcaagcaa acggctagtt accttacgca
     3361 cggttaatga atcatcaaca accatcacaa tcggtaattt aggtttttct atttgtttat
     3421 ttagatcgaa gcgttctaat ggagaatcaa gtatcaaagc ttgttctccc ttacgttgaa
     3481 atatttttat caaattaacg attaaaacaa tacttccatc cccgagtatt gtagctcctt
     3541 caacacccgg aacctgggac aactgcagtc ccgaattttt tacaacaatc tcacagttcc
     3601 caataagggt atcaacatga acagccagaa ataaatcacc attctgcagc aacagtattc
     3661 ggttatggcg cttaatttct ggtgattgat tcgttatatt caataaatta ggcaaataag
     3721 caagtgaata gcaatgacct tgaaaattga tcgattgctg ttgataagca gcagccaatc
     3781 cttcattatt aagctctaga atatgtacta cttgtgcgga aggtactgca taagtatgct
     3841 ctccgacttt taccaatact gcttgcgcga gtgcaagcgt aattggcaaa cgtaaagtaa
     3901 agctagttcc ttgattaagg gtagaagtaa cttctatttg accacccaaa tctgttattt
     3961 cattcttaac gacgtcaagc cccacacccc ttcctgcaac ttcattaact gcctgtagag
     4021 ttgtaaagcc gtgtgtaaaa agcactgact taacctgctc atcagtaata gtgtcttcat
     4081 ccgctattcc cagctgttta gctttctctc ggatcaatgc aaaatctagc ccagccccat
     4141 catcatgtaa aatgagaatg atttcattcc catcgcgact gagatcaata gtaatttgcc
     4201 cttcctctgg tttgcccaat tgcaaacgct tatccgttga ttcaatacca tgaacgatcg
     4261 cgttccttaa cagatgttca agtggagaat tgattttatc caagacactg cgatcaatct
     4321 cgatttcatc gccatgaatc gtcaaattta ctctctttcc gacatcccgt gatacttggc
     4381 gaacaacacg ataaaaatgt tcggagtaat gcttaaatgg tactgtgcga aggtgaatca
     4441 actcatgttg aacttgacta tttagtcgtg cctgctgccc caccgcatca agcgtaacac
     4501 gatgaatctc gcgcaaactt ttatgaactg tcacgatgtc atctaagctc tcggccatca
     4561 atcgtgttag ctcctggaat cgcgtaaacc gatcaagctc tagaggatca aacgcagact
     4621 gattatcaga agctgttatc acccctgatg gcatacgtgt ttcagccatt atttcaattt
     4681 cacgtaattg accccgcatc cgttctgtac tctcatccag atcttgcaag gattgtttga
     4741 agccatatag ctctgcatca acgcgagagc gcatgatact cgcttcaccg gactcattca
     4801 ccagtcgatc gataagcgca gaatccaccc taaggactgt tttggccgtt gtaaattcag
     4861 aatctattgg ttctgattga ggcgcttcaa ttacatgttc attgaaactc cgaattgcac
     4921 tttgcgccgg tacttctgca gtagtagtga caggaataat cgaaggagcg aattgagctt
     4981 ctgctgtatg cgatcgctgt aattgctcaa tatgatcatt gatttcatca aattcagcct
     5041 cgagccgatc aaaaagcgta actgataaat tttctctgga agtctgttcg accgtatttt
     5101 ccatttgatg caacaactca ccaagcgcca tagctcccgc aatacgagca cttcctttca
     5161 gcgtatgcag tgctcgtagc aggccttcac gcgcactata atcttcaaac tttgctcgcc
     5221 aaagtcttaa attacgtcca atctcaggta acaattcatg cgcttcttca ataaaaactt
     5281 cgagtaattc aagatctatt ttatcttgat cgctgattga aacatcccgc aggcccgatg
     5341 aaacaaatgc tcgttgctca gttgagattg cttgatctac caaatcctgt tcataatgaa
     5401 tatcagctac ttcagtctct tctgcttgta atttttcaag taaagtagat agctcgctag
     5461 ataagcgctt cccagtttca atattttcat cgctcggttg ctgttgattg cgaaccgacg
     5521 ataccatgct atccaaaata gaaacagtct ctttaactag tgctagcgct gattcatcgg
     5581 gcctacccga tttcctaagt aactgagtca agtattgttc caagataaca ccgatatctg
     5641 caatataggt gagcccaagc gttctcgaaa tacttgagag cgtatgcgcc gccaacataa
     5701 actcgtgttt tattggtgca ttagggttct ctgtaagatc gctcagttca gcattaagtg
     5761 tctcagcatg ttttccagct tcactagtaa acacaaaaaa caaatcatgc ggaattttaa
     5821 tattcccaac cgcaatactt gtttgcactt cagttgctaa cggtaaaggc tcgatttgct
     5881 cagttgcaac atcacgccgc acttctgttt ctatttcacc aataccaccc ttctcaggcc
     5941 ctgaggcata catcaatcgc cttgctaatt cgaagagttc tgtcggatca atttctgcag
     6001 tacctatctt tttaagacta tcgcaccaaa taccaattgt agtacggcca cattcaagta
     6061 aatcaagtag ctcctccgta gcaggatttc tttcacttaa ccatcgattc atcacttgct
     6121 ctatgcgcca agccgcttcg ctgagatcat agagcttcac catacgacca cttcctttta
     6181 aggtatgaaa gccacggcga atggctgcaa gcgcttctaa atctgctgga ttagagcggc
     6241 aagctgataa tttttcagca atactcgaca acacatcgtc ggattcctca agaaaaacag
     6301 ccaataattc agaatctcca gtctgagcca tcacaaccgt gacaggctcc aaccccgcag
     6361 atgattgcgg ttcgacaagt tcaaacgtaa cttgctcagc aatgtttggc acttcaatat
     6421 taggaactat cgattggcca aacagattta ttgcagattc aacaatttga tcacgatcga
     6481 attgtgaatt ttttagcgcc tcaatataaa aactcaaact actcaatcca tccgctagta
     6541 atgtttgttc tgtctgacta atttggtact caggatcaag taacttggta accaaatcac
     6601 gacacagtgt cagtaaaaca ttggcgcgat caagctcaag cattgagaaa acaccggcaa
     6661 tctgtttgaa tagatccggt aagaccggca aatcaacccg aatactcgga tcataaaaaa
     6721 accggtccaa aatatcctcg atctgcgcaa gactgatcaa tgattcctgc gcaatttgct
     6781 taaatagctt tttctcttga acttggtatc ccgtttcatc agggccgggc aaatcaggga
     6841 gcactatttc atcatctttc cggcttgtat cagcacgaat acgcgctgca agggcttcaa
     6901 cctgatccgg aaactctggg gataattgaa agaaattttc aatagcattt tcaacaatca
     6961 ataaagaaga agccaaatca attgctaatc gatcgcccat gttttgtgga cggatacgta
     7021 aaaaagcaac tgcaccactc atcaccgaga ttagtttttc aagtggcgca caatttactt
     7081 gtggagcaag ttgcttcaac ttatcaattg cggtcatcag tacttccaag ctttcttgat
     7141 tcccagcact atattcccgc cattgatcat ttgctccaag caaaatttca tgcatggcat
     7201 cgagaattgg ttgaattgaa tcttcatcgg gccctgccac tgcattagcc agttgatcaa
     7261 aggaggacag agatgcccgc cagtcgaaaa cattctcaat ttctttaata cgttcgctat
     7321 tggaagagct gcgcgctacg cgataaagaa tttcacgcat tagttgattc gcaaccacat
     7381 gcgcatcttt atcaaaatga cgaatctctt gctcaatttt tccgcataat cgacgtgtcg
     7441 agagatcgat cgattcatct tgatgtatca atccatccaa gaatcctgaa gacaaccacc
     7501 aaaaagttct ctgttcgata ggggcaggaa tttgctccgc caaattgacg gcatcgaaca
     7561 tttgctttag tccctcttta ttagaggtat ctttaagcca tttgagcaat cccgtctgaa
     7621 actcactccg tactttttta accgttgata acttctgaga tggatctcct tcaggtatcg
     7681 ttttaggtaa gggaaggtct tgacgaaggt caggaaaaaa taaatcgctt tcagaaattt
     7741 ctttgatgcc ctgtacttgc atcaatttcc gataaatagg caataatgtt gcaggattat
     7801 cactgatgcc atcaatgagt gcgtccaaat aacgataaat ggctctagta gcttgtttga
     7861 gtacaaccct tgtttgtgag ggagactcta ctttttcttg cagcaatgct tcaatgagtc
     7921 cctccatctt ttggctcacg aataaaaccc cttcaagctc aagcatctca agcatgccat
     7981 tgagctggtg aacataatac tgacaagact caatttgcat tctatcttca gtattctgcg
     8041 cataggcatc aatatgttta cgaattaaca caaaaatttg ttcaatcgct tctctgatcc
     8101 ctacaacaga agttatgtta attttagctt tagcaatcat tcaattaccc ttcattaatc
     8161 aaatgcgtga tacgaatcct tcgattcctt actttggttt ctaacttaat cacaatccaa
     8221 tcaaaatgat cgttaaacct taaatttggc aaccgacgct tttagctccg atgcaaatcc
     8281 agaaatctgt ttaaccagtt ttgcgttttg tgaagtgcca tcgtttgttt gccgagtaat
     8341 cgccaaaatg ttttccatat ttttagtgac tttatctacc gacttagttt gtatttgcgt
     8401 tgcatcaaat attttcgcga ccagctgcgc tagacgatta gcaatttctt caatttcttc
     8461 caacgcttca cccgtggcat tagcacgttt tgtaccttcg acaacaccgt aagtacttct
     8521 ctccatcgct gcaatcgttt cctttgtatc cccctggatc gcccgcacaa gtgaactgat
     8581 ctgtttggtc gattcagcag aatgttcagc aagccgctga acttcttgtg caattacaga
     8641 aaatcctttc cctgcctcac ctgcagacgc agcttgaatg gctgcattta atgctaaaac
     8701 attggtttgc tctgtaatat ctgaaattaa ggcaataatt tcaccgattt cttgtgaact
     8761 ttcacccaat cgtttgatac gctttgcagt ctcttgaatg gaagtacgta tttcattcat
     8821 tcccataatg gcatctctaa cagcgattgc acctttctca gctgcagtta gcgaatgttt
     8881 cgcgacattg gcgccttctt ctgcagcact cgcaaccgta ttgatcgatt ccgccatact
     8941 caaaacagaa accgttgcct cctcgatttg ccgtgactga ccgtgtgttg cagctaacag
     9001 ttgcgttgaa actcgttggg cttgggatga aacctcatta acatttgtac ccgcttgatt
     9061 gatttgcacg actaattttt gcagctcctc aaccgtataa ttaatcgaat cagcaatggc
     9121 acccgtgata tcttctgtaa ctgcagcccg caccgttaaa ttgccttccg aaagtgcgcc
     9181 catctcatct agcaacctta gaatcgcttt ttgtgctgac tcgacctcgc gctcacctac
     9241 tgccttttgc cgctgcagat aatctgaaaa tgtccggcca aaaataataa gtgatcccaa
     9301 aaccagtcca atcgaaaggt aaaaaaccaa atcctgccac gtcttagcag aagcttcttg
     9361 ctcttcgatc ttacttttta acttatcaag cgtactcagt aaaacttcat tgattttaaa
     9421 agaatcgtgc aaagcaagtt ttgcagcaat gacttcaggg gcttgtttct gtattatccg
     9481 gacttgatca tcaatcgctt ttgaaagtgc ctggattggt accaattttt ctaacacgcg
     9541 tttatcttta agcgcaggaa tacccaaatc gctatcgcca tcagatagcg ccttgagaag
     9601 taccaatagt tgttcacgct cttttgttaa ttgctcagtt atatctgaaa ttacaaattc
     9661 acctgacaac aatgtattaa cactttttgt aagacttcta gcaagcatac cgattagctt
     9721 ggttatcata acctccttcg ataatccacc aagctgctcc atttgcaccg ataattcgta
     9781 gagcaattcc tgcagtcgga tactcgctat attgatatca ttcgctattc cggcaacatt
     9841 aataagtaca tcccggctat taatcagtag gccaatttta ttttccttga tacgccacac
     9901 acgctgatat tcatcgagta attcatcaga taatgcaaca tgcatcgagg tagggagggt
     9961 ttcagggtat ttattctcgc ctcttaaaag ctccgagaac tgattcaaat aatttttact
    10021 atcttgaagt tggacaaaag caatctcatt gcctaataaa gcttgctgag aagcatttgt
    10081 caaattctga ttatagattt gcatgcggcc tatcatttca agataagatg cactttgttt
    10141 ggtgtatcgg agactcacga acatggttgc aagcgcaatg aagacaaaga tcagaaatat
    10201 gcctccatat aaccagaatc gattttgggc catccgattt ttctgtgaaa gatttatttt
    10261 ttgtccccta cttctatttg tagtttgatt ttttctgatc aattcaggtg ttaactttag
    10321 tctggctagt atcccttcca tttctttttt tctcgattgt ttgaataaca gaaactcaga
    10381 ccatcaattg ggattattct caattcaagc agaatatctg tcctacaaaa aaaccaaaaa
    10441 ttaaataaag cctaaaagta tgtccttttc ttaaaatcca aaaacagcca tgtcactcga
    10501 agtaggattt attattatcg tttgacccta gtttcttctc tcctcatact tcctgtttaa
    10561 cgccaccctt tctacaacag aaacaacttg tatgccggat tatggctctc attccagtag
    10621 cgataaccta agttatcaag aaacttctgg aaatcgactt tctcactgag tggcacttgg
    10681 ataccaacta atacgcgacc aaaatcagca ccgtgattac ggtagtgaaa caagctaata
    10741 ttccagtggt ggctcatgtt atttaaaaat ttcatcaacg cacctggcct atctggaaac
    10801 tcaaaacgat agaggatttc atgctttact tctttagagc gccctcccac catgtgtcga
    10861 acatgcaatt ttgccatttc attgccgctt aaatcttcgg tacgaagtcc gttcgcttcc
    10921 agacttttga tcaacttaac gacttcttca cgattctgaa cggacacgcc cacaaagaca
    10981 tgtgcttgct tagaatctgc ataccgatag ttaaattcgg taatactgcg tgttccgagc
    11041 atactgcaaa actttttaaa gctaccgggt tcttcgggaa tcgaaacagc cagcacagcc
    11101 tcgcgttgct cgcccagctc cgctctttcc gaaacatagc gtagtcgatc aaaattcata
    11161 tttgcaccgg atgcgacagc aaccaatgat ctatgtagca atttctcacg tgcaacatag
    11221 tgtttaagtc ccgcaactga tagcgcacca gaaggttcaa gaatcgcccg tgtatcttcg
    11281 aaaacatctt taattgcagc gcaaatggaa tcggagtcaa ccaatatcac ttcatcaaca
    11341 aactcacggc acaatcgaaa cgtctccttg ccaacatgcc tcactgccac gccatctgca
    11401 aacaaaccca cctgggaaag gttgacacgt cggccttttt gtaacgagcg atacatggca
    11461 tccgaatcaa cgggctcaac cccaacaatt tttatattag gataaagtcg tttcacgtaa
    11521 gcggcgattc ccgcaatcaa cccaccaccg ccaatcggca caaaaatggc atgaatccct
    11581 gcgccatgtt ggcgcagaat ttccattcca atcgtgccct gtcccgcaat cacatcagga
    11641 tcatcataag gatggatgaa tgtcgtttct ttctgctgcg caattgtcat ggcatgctcg
    11701 taagcatcat tatatgaatc accatgaagc aatacggtag cacccatcgc catgactgca
    11761 ttgattttga tttgaggtgt tgtagccggc atgacaatag ttgccgacga acctaatttc
    11821 tgcgcagcca gtgctacccc ttgcgcatga tttccagcgg aagcggcaat aaccccgcgc
    11881 ttaagagctg ttggtgagag ttttgccatt ttattgtagg cacccctcaa cttaaatgaa
    11941 aatacctgct gcgtatcttc tcttttcaag aagacttgat tgtggatacg tgctgataga
    12001 ttggccgctt tttctaacgg cgtttcagtt gcgacatcat acactcgggc agtcaggatt
    12061 ctttcgagat
//
LOCUS       DAOQKQ010000075        11877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_2516_length_11877_cov_5.799188, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000075 DAOQKQ010000000
VERSION     DAOQKQ010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 11877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11877
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2516_length_11877_cov_5.799188"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PKZ49_06420"
     CDS             <1..453
                     /locus_tag="PKZ49_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HND36233.1"
                     /translation="DIQVQETYYVIAHFHYVLVSGALFAIFGAVYYWLPKWCGRMYDE
                     KLGKWHFWLSMFFFNLTFFVQHFLGLAGMPRRVPDYALQFADFNMVSSIGGFGFGLTQ
                     LFFLYIVIKCIRSGEKAADKPWEGGTTLEWTLPSPAPYHSFETPPTIK"
     gene            583..1113
                     /locus_tag="PKZ49_06425"
     CDS             583..1113
                     /locus_tag="PKZ49_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HND36234.1"
                     /translation="MMYNVAKINIAMLRKLLIFSVIMFAFGYALVPFYEKFCEVAGIN
                     NLLKPDVADKDIKVDTTRLVMIQLDSNVRGLPWQFKALQSNIRIHPGESIEVMYEISN
                     NSDQETSGQAIPSYSPRVLEKYLKKFECFCFTKQVLKPGETRQMPVKFVIDPQIPADI
                     TTMTISYTFFSLGAVN"
     gene            1146..1343
                     /locus_tag="PKZ49_06430"
     CDS             1146..1343
                     /locus_tag="PKZ49_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2970 domain-containing protein"
                     /protein_id="HND36235.1"
                     /translation="MQKSEKASLWQVVKAVFWSFVGIRKKSDLEKDAEMIQPSQVVIG
                     GLIGGLFFVVSILLVVKWVTS"
     gene            1373..2221
                     /locus_tag="PKZ49_06435"
     CDS             1373..2221
                     /locus_tag="PKZ49_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HND36236.1"
                     /translation="MSHGTGHYYVPSPSKWPIVGSSALLFLGFGAAFTMNKIESGYWM
                     LAIGFAILTYLLFGWFGEVARESESGKFGKQEDKSFRWGMSWFIFSEVMFFAAFFGAL
                     YYMRQLSVPWLSDMEHQLIWPGYTGGWPTAGPGITEAFTPMGAWGLPAINTMLLLTSG
                     VTITYAHWALKKNDRSGLKKWMLATVALGIAFLCCQIYEYGHAYADLNLKLTTGAYGS
                     TFFMLTGFHGFHVTVGTIMLIVIYFRCAAGHFKPEHHFGFEGVAWYWHFVDVVWLGLF
                     IFVYLL"
     gene            2339..3061
                     /locus_tag="PKZ49_06440"
     CDS             2339..3061
                     /locus_tag="PKZ49_06440"
                     /inference="COORDINATES: protein motif:HMM:NF014193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HND36237.1"
                     /translation="MIIKDYQFKPKWWILVLSVILIIIFTRLGLWQLSRADEKDSKQQ
                     LLDQLAHEAVLTLPSSRVELSDLLFRNVEVRGQFIASHTIFLDNKVYRGAVGYHVLTP
                     MRIENSNMHVLINRGWVALGKNRSTLPEIFTPTDVITLSGIALSPTIRTMKISDEIST
                     GKVWLAFNFEEYQAASKLELQPILLMQLDNSINDGLIRELERQDLGASKNMSYAFQWF
                     FLAITVFILLLALNVKRSSPQQ"
     gene            3033..3617
                     /locus_tag="PKZ49_06445"
     CDS             3033..3617
                     /locus_tag="PKZ49_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36238.1"
                     /translation="MSNEVARSNRTKFIALILLMLSPVMASVILHNIKFRPDSTVNYG
                     ELLEVKAIHGQAHEIENNTIFRERQLHGKWSFAMIDSGNCEEYCQKKLYQMRQVRLAQ
                     NTEKDRINRIWFIDDQIVPNQEIKKEFEGTVLLTVNDNKFLDQFPAVSSRKDHIYVID
                     PMGNLMMRYPRDANPSKMVNDIKLLLKLSHLEHK"
     gene            3651..4547
                     /gene="cyoE"
                     /locus_tag="PKZ49_06450"
     CDS             3651..4547
                     /gene="cyoE"
                     /locus_tag="PKZ49_06450"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HND36239.1"
                     /translation="MSVNTSTSQSVLDRLQQFYRLTKPRVVSLIVFTAVIGMFLAVPG
                     AVPLDTLVLGTIGIAFVAGAAAALNCLVEQKFDAVMARTRGRPLPQGKVSVPETLFFL
                     GLIGGLGLFILHQWINPLTMWLTLGTFVGYAIIYTVILKPLTPQNIVIGGASGAMPPV
                     LGWAAVTGEVTADALLLFLIIFAWTPPHFWALALYRKTEYAQIGMPMLPVTHGDKFTR
                     LHVLLYTLILCVVTILPYVSQMSGLIYLFSAIVLDAIFFYYAIKIYLNYSDQLARAAF
                     KYSILYLALLFTALLVDHYFYI"
     gene            complement(4661..6028)
                     /locus_tag="PKZ49_06455"
     CDS             complement(4661..6028)
                     /locus_tag="PKZ49_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HND36240.1"
                     /translation="MLPSELVAQIQKIIPSERFYTDPVDCYAYGYDNSRKIFPPEAVV
                     FPITTDEVVAIVSLCNRYKIPITPRGRGTGTAGGSIPDQGGIVLSLERMVKILNIDPG
                     NRVVVSEPGVLNQEIQDTVKPYGFFWPPDPSSANFSSIGGNLATSAGGPHAVKYGTTR
                     DHVLGIKAVTATGDIVKTGCYTTKGVVGYDLTRLLIGSEGTLAVITEATLKLTPLPKC
                     KSGLVAYFQDATSCAKAIVAIMSLPQGPSALEFLDKNSLNLIRNRFPELIPIEANTLL
                     IIEVDGSENELSEAMATIKSSCDIDGILSIEHSTNPHDLWKARKALSPLLRDIAPKKI
                     NEDVVVPVNSLPQFLDDLDKICTKFQIANVNFGHAGNGNIHVNLLIDPNNSDENLRAE
                     ACLDEIFDLVIALKGTLSGEHGIGREKRAFIHKEIDDPTLTIMKSIKTLFDPNNILNP
                     GKIFP"
     gene            complement(6061..6657)
                     /locus_tag="PKZ49_06460"
     CDS             complement(6061..6657)
                     /locus_tag="PKZ49_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HND36241.1"
                     /translation="MNFIKINLLIPFTLSLSCALWFSGCVPLVATGVGVGAGTGALMS
                     EDRRSTGIFIEDEAIELKTSRRITQQLGGNVHINVTSFNRNVLLTGEAPNEATQSEIE
                     KLALSIDNVRKVFNEITIAENSTLSSRTNDSIITSKVKGRFVSERKFRVNYVKVVTEN
                     SVVYLLGLVTREEGEDASQIASTTSGVTRVVKVFEYLN"
     gene            complement(6827..7003)
                     /gene="ssrS"
                     /locus_tag="PKZ49_06465"
     ncRNA           complement(6827..7003)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PKZ49_06465"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            7287..9665
                     /gene="ppsA"
                     /locus_tag="PKZ49_06470"
     CDS             7287..9665
                     /gene="ppsA"
                     /locus_tag="PKZ49_06470"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HND36242.1"
                     /translation="MKAYIKWFDELSMRDIALVGGKNASLGEMYSHLTKLGVNVPAGF
                     ATTTEAYRDFLEANDLSQRIKHELDQLDVDDVATLAITGKTVRAWLIEAVFPDQLLEA
                     ISIAYEKLEKSAGGEAVSFAVRSSATAEDLADASFAGQQETLLNVSGLDQIIQAIKIV
                     FASLYNDRAIAYRVHQNFPHDKVYLSVGIQKMVRSDLGASGVMFTLDTESGFQDAIFV
                     TSAYGLGETIVQGIVNPDEFYVYKQGLRSGKSAILSRRLGTKLIEMVYENSFKQDTFT
                     VTRDVSVERRQRFSLSDQQIEQLGQQALLIEQHYGRPMDIEWALDGLDGKLYIVQARP
                     ETVKSRAGTSLERYHLTQKGMVLSEGRAIGQKIGQGTAKVIFSIDQMHEIQSGDVLVT
                     DMTDPDWEPIMKRAAAIVTNRGGRTCHAAIIAREMGIPAVVGCGEATSQIKPGSSVTI
                     SCAEGDTGYVYEGLLAFEHSVADMGQLPQLPIKMMMNVGNPSHAFAFSQIPNQGIGLA
                     RLEFIISNMIGIHPKAVLEFDQLPSDLQALILGRSAGYANPVQFYIEKLVEGIATLAA
                     AFYPNPVIVRTSDFKSNEYANLIGGERYEPKEENPMIGFRGASRYISPQFQACFELEC
                     QALRKVRDEMGLTNVEIMIPFCRTLEEAQQVTALLASQGLSRGRNGLRVIMMCEIPSN
                     ALLAEEFLQFFDGFSIGSNDMTQLCLGLDRDSSLVAHAFDERNPAVKKILKMAIDACR
                     TQGKYIGICGQGPSDYPDFAAWLNEVGISSLSLNPDSIVETWLYLASLTKQN"
     gene            9668..10501
                     /locus_tag="PKZ49_06475"
     CDS             9668..10501
                     /locus_tag="PKZ49_06475"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HND36243.1"
                     /translation="MIKQRRTVFFLSDRTGITAETLGHSLLTQFDGIEWVKVSIPFLD
                     EIGKIKVAIDQINQIAVQEGVRPLVFSTLLNPEILEQVRQANCRVYDFFAMFMGSIEQ
                     ELNQPFARIAGRSHGLQHHLTYFKRINAINYVLAHDDGVNPKNFAEADIILIGVSRTG
                     KTPTCLYLAMQYGIAAANYPLTQEDMGSMRLPKVLESVQDKLFGLTLSAAQLHLIRQE
                     RRPNSEYASLAQCQHEIQWQQSLFKLMNIPYLDTTAISIEEISAVILDRCGLKRQLYG
                     L"
     gene            10697..>11877
                     /locus_tag="PKZ49_06480"
     CDS             10697..>11877
                     /locus_tag="PKZ49_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36244.1"
                     /translation="MNKLEKYKQKYFRGFLFFLSLWFLINPLKALELSPYAEGPLLEI
                     ITQMPEGTWKKVNANSFSEVWTPPELRPLNLLSNPPPSKLISAWSSFAWDSNRGDLIL
                     YGGGHANYSGNDVYRWRSRDLKWERASLPSQIAVIAGTSASTAIDGVDAAPASAHTYD
                     NSIFLPIIDRYLVLGGALYNTGGPYIRQSETDPTVTRITGPYLFDPEKASANQVGGTT
                     GSHVQRVYAFPEITGGEMWQNRDIHKHLATSILPKHHVNGCTAYANESGVDTVYISAS
                     GSIHGTGQSLFRYQITDVNSPWTDKIDRVAIYWGAPSDQPSCGYDSDTKIFLKTTGVN
                     TKPLYFWDLRPERTTNKDQAVVVEGSISNLVQWLNNYTASTGKSLRHCALDFDPNRKN
                     F"
BASE COUNT         3467 a         2123 c         2567 g         3720 t
ORIGIN      
        1 gatattcaag ttcaagaaac ctactatgta attgcacatt ttcactatgt gcttgtatct
       61 ggtgcgctat ttgcaatctt tggtgcagtt tattattggc tgcctaaatg gtgtggtcgt
      121 atgtatgatg aaaaattggg taaatggcat ttttggttat ccatgttctt cttcaatttg
      181 actttctttg tacagcattt cttaggatta gcaggtatgc cacgcagagt tcccgactac
      241 gctttacagt ttgctgattt taatatggta tcaagcattg gtggttttgg atttggttta
      301 actcagctat tttttctgta tatcgtaatc aagtgtattc ggagtggaga gaaagctgct
      361 gataaaccat gggaaggtgg aacaacctta gaatggacat taccgtcacc agcaccttat
      421 catagctttg aaacacctcc taccatcaag taattaatgt gaggagagta tggattcaca
      481 ggatgataaa gatagaaagc acatttggag aaatgttaaa acagcgtttt ttctattggc
      541 aatgatggtt ttgatattta ctgtaactgt tctaatcaag caatgatgta taacgtagcc
      601 aagattaata tagcaatgtt gagaaaatta ctcattttct cagtgattat gtttgccttc
      661 ggttacgcgc tggtaccgtt ttacgagaaa ttttgtgaag ttgccggtat caataatcta
      721 ctgaaaccag atgtggctga taaagacata aaggtcgata caacacgtct ggtaatgatt
      781 caactagatt caaatgtacg tggattgccc tggcagttta aggcgttaca gtcgaatatt
      841 agaattcatc ctggtgaatc catcgaggta atgtatgaaa ttagtaacaa ttcagatcaa
      901 gaaacaagtg gtcaggcgat acccagttat agtccgcgcg ttcttgaaaa gtatttgaag
      961 aaatttgaat gtttttgttt cacaaagcaa gtgctaaaac caggtgaaac tagacaaatg
     1021 ccagttaaat ttgtaattga cccacagatt cctgcagata taacaacgat gacaatttct
     1081 tatacttttt tctcgcttgg cgcggtaaat tagaatagga aaagtaaatt tggatgaact
     1141 tataaatgca gaaatcagag aaagcttcat tgtggcaagt tgttaaagca gtgttttggt
     1201 cgtttgttgg aataagaaaa aaaagtgatt tagaaaaaga tgcagaaatg attcagccta
     1261 gtcaagtagt aatcggggga ttgattggtg gattgttttt tgtagtcagc atcttattag
     1321 tagttaaatg ggtaacaagc tagttgatta aaataaattc aggaggaatg atatgagtca
     1381 cggaaccggt cattattatg taccgtcacc atccaaatgg cccattgtag ggtcgtcagc
     1441 actgctattt ttagggtttg gtgctgcatt taccatgaac aagattgaat cgggttattg
     1501 gatgctagca attggttttg ccatactaac ctatttgtta tttggatggt ttggtgaggt
     1561 tgctagagaa agtgaatcgg gaaaatttgg taaacaagaa gataaatcat ttcgttgggg
     1621 aatgagctgg tttatctttt ctgaagtaat gttctttgca gccttcttcg gcgcacttta
     1681 ctacatgaga cagctttcgg taccttggtt atcggacatg gagcatcagt tgatatggcc
     1741 gggatatact ggaggttggc caacagcagg tcctggaatt acggaggcat tcacaccaat
     1801 gggtgcttgg ggtttgccag caattaatac gatgctgcta ttgacttcag gtgtgacgat
     1861 tacttatgct cattgggcgc ttaagaaaaa tgatcgcagt ggcttaaaaa aatggatgtt
     1921 agcaacggtt gcacttggta ttgcattctt gtgttgccag atttatgaat atggtcatgc
     1981 atatgcagat ttaaatctta aattgacgac gggtgcttat gggtctacct tcttcatgtt
     2041 aactggattt catggtttcc atgtaacagt cggtactatt atgttgatag tcatctattt
     2101 cagatgtgca gcaggtcact ttaagccaga gcaccatttt ggatttgagg gtgtggcctg
     2161 gtactggcac tttgttgacg tagtttggtt aggtttattt atatttgtgt atttgttata
     2221 agtactaata aatgttgtga agtaaggaaa atacatcata ctgatagcga tatcagtatg
     2281 attataaaaa tagaaaaaga gaataattat aagtagaagc ctagaagttg attgatgaat
     2341 gattatcaaa gattatcagt tcaaaccaaa atggtggata ctagttttat cagtaatatt
     2401 aattattatc tttacacgat taggtctgtg gcagctttct cgtgcagatg aaaaagattc
     2461 gaagcagcaa ttactagatc agttagcaca tgaagcagtt ttaacactgc cttcttcgcg
     2521 ggttgagttg agcgatctat tatttcggaa tgtagaagta aggggtcaat ttattgcaag
     2581 ccatacaata ttccttgata acaaagttta tcgaggtgct gttggatatc acgtgttaac
     2641 accaatgcga attgaaaata gtaatatgca tgtgttaatt aatcgtggct gggtggcact
     2701 tggtaaaaac cgatctacat tgcctgaaat ttttacgcca acggatgtaa ttaccctttc
     2761 tggcatcgct ctttctccaa caatcaggac gatgaaaatt tccgatgaaa tttctaccgg
     2821 taaggtatgg ttagcgttta attttgaaga atatcaagca gccagtaagt tagaactaca
     2881 acccattctg ctcatgcagc tcgataattc aattaatgat ggattgatac gtgagctaga
     2941 acgacaagat ttaggtgctt ctaaaaacat gagttatgca ttccaatggt tctttttagc
     3001 aattactgtt tttattttat tattagcatt aaatgtcaaa cgaagtagcc cgcagcaata
     3061 ggacaaaatt tatagcattg atattgctta tgctatcgcc cgtaatggcg tcagtgattt
     3121 tgcacaatat taaatttcgc cctgatagca ctgttaacta tggagaattg ctagaagtaa
     3181 aggcaataca tggccaagcg catgagattg aaaataatac gatatttcga gaaagacaat
     3241 tacatgggaa gtggtctttt gctatgatcg actccggcaa ctgtgaagag tattgtcaaa
     3301 aaaaattgta tcaaatgcga caagtacgtt tagcccagaa tactgagaaa gacagaataa
     3361 atagaatttg gtttattgat gatcaaatag taccaaatca agaaatcaaa aaagaatttg
     3421 aaggaacagt attattaact gttaacgata ataagtttct ggatcaattt ccagccgttt
     3481 cttcaagaaa agatcatatc tatgtcattg atcctatggg aaatttaatg atgcgttacc
     3541 ctcgggatgc taatccatca aaaatggtaa atgatattaa actgttattg aaactatctc
     3601 atttagaaca taagtaattt gaattgtatt ttaggagggt aatataagat atgagtgtta
     3661 acacaagtac aagccagtca gttttagata gactccagca attttaccgt ttaacgaaac
     3721 ctcgtgttgt ttcgttgatc gtttttactg ctgtaatagg aatgtttcta gcagttccgg
     3781 gtgcagtacc acttgatacc ttagtgcttg gaacaattgg aattgctttt gttgccggtg
     3841 ctgccgctgc actaaattgt ttagttgaac aaaaattcga tgcggttatg gctagaacaa
     3901 gaggaagacc actgccgcaa ggaaaggtaa gtgttcccga gactttattt tttcttggat
     3961 taattggtgg gttagggtta tttatcttgc atcaatggat aaatccacta acaatgtggc
     4021 taacattagg tacgtttgtt ggctatgcaa taatttacac tgttatctta aagccactta
     4081 cgcctcagaa tattgtgatt ggtggtgcct caggtgctat gcctcctgtc ttaggctggg
     4141 ctgctgtaac aggggaagta acggcagatg ctctattgct gtttttaatt atttttgctt
     4201 ggacacctcc gcatttctgg gctttagcac tgtatcgcaa aacggaatat gcgcaaattg
     4261 gtatgccgat gctaccggta actcatggcg ataaatttac ccgcctccac gttttgcttt
     4321 ataccttgat tttgtgtgtt gtgacgattc tgccttatgt ttcgcagatg agtgggttga
     4381 tctatttatt tagtgcaata gttttggatg caatattttt ctattatgcg ataaagattt
     4441 atctcaatta cagtgatcag ctagcacgcg cagcgtttaa atattcaatt ctttacttgg
     4501 ctttattatt tacagcatta ctcgttgacc attattttta tatctgatgt agagaaaagt
     4561 tactagctaa cgagaaaacc ttacacaaga tgattcttgt gtaaggtttt tttatagcct
     4621 gaaatgctta tcaccgtaac gtaaagtgtt gcccgctaga ttagggaaaa atttttccag
     4681 gatttagtat attgtttgga tcaaataggg ttttgatact tttcatgatc gttagcgtag
     4741 gatcatcaat ctccttatgt ataaaagcgc gtttttctct accaatacca tgttcacctg
     4801 ataatgtccc tttgagcgcg attactaaat caaatatctc atcgagacaa gcttccgcgc
     4861 gtaaattttc gtctgaatta tttggatcga tcagtagatt cacatggata ttgccattac
     4921 ctgcatgccc aaaattaaca ttcgctattt gaaatttggt acagatttta tctaaatcat
     4981 ctagaaattg aggtagtgaa tttactggaa ctacaacatc ctcgttgatt tttttgggcg
     5041 caatatccct taagaggggt gataatgctt tccgagcttt ccataaatca tggggattag
     5101 tgctgtgttc aatcgataat atcccgtcaa tatcacagct agactttata gtagccatcg
     5161 cctcagataa ttcattttcc gaaccatcaa cttcaatgat taacagggta ttagcttcga
     5221 ttggtatcag ctcagggaat cgattccgaa taagatttag tgagttttta tctaaaaatt
     5281 caagtgcact aggcccttga ggtaaactca taatggcaac aatggctttg gcacagctgg
     5341 tcgcatcttg aaaataagcg actaatccac ttttgcattt gggtaacggt gttaatttca
     5401 gtgtggcttc agtaataaca gctagtgttc cctctgatcc aattagcaat cgagttaaat
     5461 catagccaac cactcctttg gtggtatagc agccggtttt tacgatatct ccagtagcag
     5521 ttactgcctt tatccctaaa acgtgatcgc gtgtcgttcc atactttacc gcatgagggc
     5581 cccctgcgct tgttgccaaa tttccgccta tgctcgaaaa attagcactc gatggatcag
     5641 gtggccagaa gaagccgtaa ggctttactg tatcttgaat ttcttgattt aatacgcctg
     5701 gctctgagac aacgacacga tttcctgggt ctatgttaag tatttttacc attcgttcaa
     5761 gtgataatac gattcccccc tgatcgggta tgctacctcc ggcggttcca gttcctcttc
     5821 ctcttggtgt aattggtatt ttgtatcgat tacataatga aacgattgct acaacttcat
     5881 ctgtagtaat tggaaaaaca acagcctcag gcggaaaaat ttttcgacta ttatcgtatc
     5941 cataggcata gcaatcgaca gggtccgtgt agaagcgttc cgaaggaata attttttgga
     6001 tttgtgcaac taactcagat ggtagcactg aataaatgag cgaagacgtg atcagtaatg
     6061 ctaatttagg tattcaaaca cttttacaac gcgagtcaca ccagaagtcg tgctagcgat
     6121 ttgtgaggca tcttcacctt cttctcgagt caccaaacca aggagataga ccacactatt
     6181 ttctgtcacc actttaacat agttaacccg aaattttctc tcactgacaa atcgaccttt
     6241 taccttggac gtgatgattg aatcattcgt tctagatgat aaagtgctat tttcagctat
     6301 ggtgatttca ttgaaaacct tgcgaacatt atcaatactc aacgcaagtt tttcaatctc
     6361 actttgggtt gcctcgtttg gcgcttctcc agtgagaagt acatttcgat tgaagctagt
     6421 tacattgata tgaacatttc caccaagttg ctgagtaatt cttcgactcg tttttaattc
     6481 gattgcctca tcttcaataa aaatacctgt acttcggcga tcttcactca tcagagcgcc
     6541 cgtaccagcg cctacaccaa caccggttgc gacaagcggt acacagccag aaaaccataa
     6601 agcacaagac aaactaagtg taaaaggaat caataagtta attttgatga aattcataag
     6661 gttaactatg ctagattatt aatttttggt attcaggtga tataaacaac ttaaaccatt
     6721 atcttctgct aaagcattag cttcaagtta aatgatttga atgagttaat tataagcaac
     6781 tatctgttaa aaattccaga ctactctgaa ggaatatttt gagaggtagc cccccgcaga
     6841 tgccgttaga ccgatatctt gaacctttgg gtccaaggtg gccacaagca gtttgcatca
     6901 ggttcgtcta aaaagacgat cacaacagca ttaatcgagc tcgcagccaa atttcaagaa
     6961 ttggttcaaa gaatttgagc cctcacgaac accgcagggg agaagcgtta gaagcaaaag
     7021 ctagctacat cggtataagc caataatgcc aacctttgac ctaatgatgc tgctacaaga
     7081 tccaatttca ctaacttgtt cctttaaaat tattctacgc tttaattcac ccatgtcaaa
     7141 acttttaaat taggtaatga gtgtgtaagt acttaagctt atcaatatct caaaatgttt
     7201 caaatgaacc cttatcaatc cccatttata cttatagtga taagattatt aattacgagc
     7261 aatcaattga ctggtcatga ttcgagatga aagcatatat taagtggttt gatgagttga
     7321 gtatgcgcga tattgccctt gtagggggga aaaatgcatc gcttggagag atgtatagtc
     7381 atttaacgaa attgggagtt aatgttcccg caggatttgc aaccactact gaagcttacc
     7441 gggatttttt agaagcgaat gatcttagtc agcgtatcaa acacgaactt gatcaactag
     7501 atgtcgatga tgttgcaacg ttggcaatca ctggtaaaac cgttcgggcg tggttgatcg
     7561 aagctgtgtt tccggatcag ttattagaag ctatttcaat tgcctatgag aagcttgaga
     7621 aaagcgctgg cggtgaagca gtttcatttg ctgtccgatc atcagcaact gctgaggatc
     7681 ttgccgatgc ttcatttgcg ggtcagcagg aaacgctact gaatgtaagt gggctagatc
     7741 aaattattca agcaatcaaa atcgtgtttg catcgctcta taatgatcgg gctattgcat
     7801 atcgtgttca ccaaaatttc cctcatgata aggtttatct ttcagtgggt atccagaaaa
     7861 tggtgcgcag tgatctgggg gcaagtggcg tcatgtttac tttggatacg gaatcgggtt
     7921 tccaagatgc aatttttgtt acatcggctt atggactggg tgaaacgatc gtgcagggca
     7981 ttgtcaatcc tgatgagttt tatgtttata aacaagggct gcgttctggt aaatctgcaa
     8041 tcttgtcacg acggcttggc actaaattga tcgagatggt ttatgagaat tccttcaagc
     8101 aggatacatt tactgtaact cgggatgttt ctgttgagcg tcgtcaacga ttctcattat
     8161 ctgatcaaca gattgagcaa ttgggtcagc aggccttatt gatcgaacag cattatggaa
     8221 ggcctatgga tatcgaatgg gcgctcgatg ggttagatgg aaagctatat attgtgcagg
     8281 ctagaccaga aaccgttaaa agccgagctg gcacatcgct agaacgatat cacttaactc
     8341 aaaaaggaat ggttctatcg gaaggtcgcg caattgggca aaaaatcggt caaggaacag
     8401 cgaaggtaat tttcagtatc gatcaaatgc atgagattca atccggtgat gtattggtca
     8461 ctgatatgac ggatcctgat tgggagccta tcatgaagcg cgctgcagcg attgtgacga
     8521 accgtggtgg aagaacatgt catgcggcaa ttattgcgag ggaaatgggc attccggctg
     8581 ttgtgggttg tggtgaggcg acttctcaga taaaaccggg tagttcagtt acgatttcat
     8641 gcgcagaagg ggataccggt tatgtttatg aaggcttatt agcattcgaa cattcggtag
     8701 cagatatggg tcagttaccg caactaccaa ttaagatgat gatgaatgtt gggaatcctt
     8761 ctcatgcatt cgcattttcg caaataccca atcaaggtat tggattagcg cgtctagagt
     8821 tcattatcag caatatgatt ggtattcatc ccaaagcagt gttggaattt gatcaattgc
     8881 catccgatct gcaagcacta attcttggcc gtagtgcggg atacgcaaac cctgttcaat
     8941 tttatattga aaaattggta gaagggattg cgacgcttgc tgctgctttt tatcctaacc
     9001 cagtgattgt gcgtacatcc gattttaaat ccaatgagta tgcgaattta atcggtggtg
     9061 agcgctatga gccaaaagag gaaaacccaa tgattggttt ccgtggtgct tctcgttata
     9121 tttcaccgca gtttcaagct tgtttcgagt tggaatgcca ggcgttacgc aaagttcgcg
     9181 atgaaatggg gttaaccaat gtggaaatta tgattccttt ttgtcgtact ttagaagaag
     9241 cacagcaggt tacagcatta ttggcatctc aaggactttc tcgagggaga aatggcttgc
     9301 gtgtaatcat gatgtgcgaa attccttcaa atgctttgct agccgaggaa tttttacagt
     9361 tctttgatgg gttttcgata ggatcaaatg atatgacaca actgtgtctt ggacttgatc
     9421 gcgattcgag tttagttgct cacgcatttg atgaacgaaa tcctgccgtt aaaaaaatac
     9481 tgaaaatggc catcgatgct tgtcggactc aaggcaagta tattggtatt tgtgggcagg
     9541 gaccctctga ctatcctgat tttgctgctt ggcttaatga agttggtatt agtagccttt
     9601 cgttaaatcc cgattcgatt gttgaaactt ggctttattt ggcaagcctg acaaaacaaa
     9661 attaagtgtg attaaacaaa ggcgtactgt tttttttctt tctgaccgaa caggtattac
     9721 cgctgaaaca ttaggtcata gcttgttaac acaatttgat ggaatcgagt gggttaaagt
     9781 tagcattcca tttcttgatg agattgggaa aatcaaagta gctatcgatc aaatcaatca
     9841 aattgcagtg caagagggtg ttcgcccttt agtttttagt actttactca atccagagat
     9901 cttggaacag gttcgccaag ccaattgccg cgtctatgat ttctttgcaa tgtttatggg
     9961 ttcgatcgag caagaattaa atcaaccttt tgcgcggatt gctggacgtt ctcacgggct
    10021 tcagcaccat ttgacttatt tcaaaagaat aaacgccatt aactatgttt tggcgcatga
    10081 tgatggcgtt aatcctaaaa attttgctga agcagatata attttgattg gggtatcgcg
    10141 aaccggtaag acaccaactt gcctttattt agcgatgcag tatggaatcg ctgctgcaaa
    10201 ttatccacta actcaagagg atatgggctc gatgcgatta cccaaagtgc tggaatccgt
    10261 tcaggataaa ttgtttggct taacgctcag cgcagcccag cttcatttaa ttagacaaga
    10321 gcgccgtccg aatagtgagt atgcttcact cgcacaatgc cagcacgaaa ttcaatggca
    10381 acaatcattg tttaaattaa tgaatattcc ctatttagat accaccgcga tttcgattga
    10441 ggaaatcagc gcagttattt tggatcggtg tggattaaaa cgccagcttt atgggttata
    10501 gcagaggcat caaagcgatt acgcctaaaa aagatcattg gtagttgata cgcattgagt
    10561 taaagcagtg aaaaaaatca aattcgttga ctattctgat attctggcta tagatcaata
    10621 aaattgctcc ggcttgttga catttcacat agatatcact agcttatcaa gaaaacaaca
    10681 gaataagggt aaaaaaatga ataagttaga aaaatataag caaaaatatt ttcgaggttt
    10741 tttatttttt ctgagcttgt ggtttttgat aaatccacta aaagcgctag agcttagtcc
    10801 ttatgcagag ggtcctctgc tcgaaataat tactcaaatg cctgagggta catggaaaaa
    10861 agtcaatgca aattcttttt cagaggtctg gacgccgcct gaattaaggc cgctcaatct
    10921 tcttagtaat cccccaccaa gtaaactgat ttcagcatgg agtagttttg catgggatag
    10981 taatagagga gatttaattc tttatggtgg tggacatgca aattattcag gtaacgatgt
    11041 ctaccgttgg cggtctcggg atttgaagtg ggagcgtgct tcgttaccga gtcagattgc
    11101 agttattgca gggacttcag cgtcaactgc cattgacggt gtagatgctg ctcccgcttc
    11161 agcgcatact tacgataaca gtatttttct tccaattata gatcgatatc ttgtattagg
    11221 cggagctttg tataatacgg gtggtcccta tattcgccaa agtgaaaccg atcctacagt
    11281 tacccggata acaggcccgt atttatttga tccggaaaaa gcttcagcaa atcaagtggg
    11341 tggtactacg ggatcccatg tgcagcgtgt ttatgctttt cctgagatca ctggtggtga
    11401 gatgtggcag aacagagata ttcacaagca cttagcgaca tcgatcttac caaaacatca
    11461 tgttaatggc tgtacggctt atgcaaatga atcaggggtg gatactgttt atattagtgc
    11521 gagcggaagt atacatggta cagggcagtc gttgtttcgc tatcaaatta ctgatgttaa
    11581 ctcgccttgg actgataaaa ttgatcgtgt agcgatttat tggggagcac cttcagatca
    11641 accatcatgt gggtatgatt ctgatactaa aattttcttg aaaacaacag gtgttaatac
    11701 aaaaccttta tatttctggg atttacgacc agaacgtacc acaaataaag atcaagcagt
    11761 cgtggttgaa ggttcaattt ccaatctggt tcaatggtta aataattata cggcttcaac
    11821 cggtaaatca ctccggcact gcgcacttga ttttgatccc aatcgtaaaa acttttt
//
LOCUS       DAOQKQ010000076        11603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_2603_length_11603_cov_6.365345, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000076 DAOQKQ010000000
VERSION     DAOQKQ010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 11603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11603
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2603_length_11603_cov_6.365345"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..1469)
                     /gene="murD"
                     /locus_tag="PKZ49_06485"
     CDS             complement(54..1469)
                     /gene="murD"
                     /locus_tag="PKZ49_06485"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HND36245.1"
                     /translation="MIFHEKKVLVLGSGETGISMVKWLSYLGAHLSLADSRANPPNPN
                     FLDDFIPHQKRFFGHFDLNLLENIDAIAISPGVPLAEAFVQEAIKRNIPVFGDIELFA
                     LTLNQLNQPKPIILAITGSNGKTTVTSMVGDMVKAAGWDVEVAGNIGPAVLKVLLQRL
                     STKGLPQCWVLELSSFQLETTQTLFANSAVVLNISEDHFDRYDGLDDYAAAKARIFIG
                     GTHSTQILNRDDPNVLAMALPDKKQVTFGLSKPSRDVDFGLLEFGSALWLVEGKRRLL
                     KADMLKIPGMHNVANALAALALCRSVGLPHEPLVDALLKFRGLPHRMQKIATIQGVDY
                     YDDSKSTNVGSAIAALNGMKGNIVLIAGGDGKGQDFSPLIEPIKQYVRALVLLGRDAE
                     KISQAIKGAAKPIHHVSTMEDAVRLSFLLAKPNDMVLLSPACASLDMFRNYIHRAEVF
                     TVAVKELESKGISLVESSPSL"
     gene            complement(1466..2551)
                     /gene="mraY"
                     /locus_tag="PKZ49_06490"
     CDS             complement(1466..2551)
                     /gene="mraY"
                     /locus_tag="PKZ49_06490"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HND36246.1"
                     /translation="MLLLIAQWIAQDVRVFNVFNYITLRTVLAVLTALVISFVIGPAM
                     IRKLTAYKIGQSIREDGPQSHLVKAGTPTMGGALILTSIVITILLWADLTNPYIWVVL
                     LTTLGFGVIGWVDDYRKVVHRNSKGLSAAAKFFWQSVIACCVAAYLYWIADTPEQTNM
                     IVPFFKEVAIPLGMVTFIVLTYLVIVGTSNAVNLTDGLDGLAIMPTVMISGALAIFAY
                     VTGHIVFAKYLGIPYIQNVGELAVFCGALAGAGLAFLWFNTYPAEVFMGDVGALSLGA
                     ALGVITVIVRQEIVLVIMGGVFVAEAMSVMLQVASFKLRGKRIFRMAPIHHHFELKGW
                     KENQVVVRFWIITLILVLLGLSTLKLR"
     gene            complement(2609..4000)
                     /gene="murF"
                     /locus_tag="PKZ49_06495"
     CDS             complement(2609..4000)
                     /gene="murF"
                     /locus_tag="PKZ49_06495"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HND36247.1"
                     /translation="MMTVQEAARMLNADWHGDDLVFGDVSTDSRQIKAGDLFVALSGD
                     RFDGHDYIGDTIERGAVAALVAREIKKTKLPQHFGWIHVANTRLALGQLASQWRDRFS
                     LPLVAVTGSNGKTTVKEMIASIFTKAASRQSNFTTDASVDQVLATIGNLNNDIGVPLT
                     LLRLRQHHVYAVVEMGMNHAGEIAYLTQLAKPDIAVITNAGAAHIEGLGSIELVAHAK
                     GEIFQGLDKPGVAIINADDKYAQLWYQLAGNRQIISFGMNAKAQIRADCHLLASSNKW
                     ILKFPKEESEIELQVLGQHNVMNALAAAAAALGAGVSEQVIIEGLSAFSGVKGRMQKK
                     SGLHQATLIDDTYNANPASVSAALSVLAHLSGRKIFVLGDMGELGVGGADLHRKIGEE
                     ASRAGIEKLLALGELSVNAAEGFGKGAMHFSNIDELLEEAEKLLDHNVTMLVKGSRFM
                     KMERVIQRFEVRH"
     gene            complement(3997..5559)
                     /locus_tag="PKZ49_06500"
     CDS             complement(3997..5559)
                     /locus_tag="PKZ49_06500"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HND36248.1"
                     /translation="MSSLISWKSDELLVDRILAELNVPIKRLVTDYRHIKAGNTFLAY
                     AGDRYDARSDIAKVIAAGANAVIWEKQQFSWNPEWVIPNCAVLDLKEKAGLISSRVYG
                     NPSRHLWLVGFTGTNGKTSCSHWYAEAMTMLGKKTAVIGTLGNGFLQDLKYTANTTPD
                     AVLLQETLASCLKEGAQNLAIEVSSHGLVQGRVNGCEFAVAVLTNLSRDHLDYHKNMD
                     EYAAAKAKLFFWPNLKCAVLNLDDVFGIELVQQIGKRDLQIIGYGFNQSIDLKLNQGN
                     LRVLSGNNLQATIDGLEFDVEFEHEKAHIKTTLFSRFNANNLLAVIASLIASGVNLQD
                     AVAVLSRLKPVPGRMEKFGGGAQPTVIVDYAHTPDALEKVLTGLRETLVNSHLKQRPH
                     SRKARLICVFGCGGDRDRGKRPIAGGIVTRLADQVIVTSDNPRNEDARDIINEIVAGT
                     RYQNYIIEEDRASAIYQAIHNAHKNDIVLIAGKGAEAYQEIKGKRLPFDDREVTQQVL
                     HDLANMKMRVRR"
     gene            complement(5556..7286)
                     /locus_tag="PKZ49_06505"
     CDS             complement(5556..7286)
                     /locus_tag="PKZ49_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HND36249.1"
                     /translation="MRYQTAATAIYLPVWRSRFLIIVLLLSLIALSGRAIYLQSLNKD
                     FLKQQGESRYSRVVELNAQRGKITDRNGEILAISAPVKSVWVDPQQLNVTPDQLIKLA
                     QILEVDVAEIHKRISDKDKRFVYLKRQLPPETAENIADLNIKGLYLKREFYRYYPTGE
                     MAAHVLGFTDIDGKGQEGVELGWEGILTGKHGSRRVIKDRIGRVVENVERIRSPQPGQ
                     NLVLSIDSKIQYLAHRELKQAVESHRAKAGSVIILDAQTGEVLALANYPTYNPNQRSS
                     IRKESLRNRAFVDTFEPGSTLKPFAVSIAMETGKIKPTSLLETAPGFLKVGKATIRDV
                     RNKGALSVSQVIQESSNVGTAKIALMLPPKTFWEMLSQSGFGTSTGSGFPGEVNGVLR
                     PYQKWKPIEQVTMSYGHGISVNLMQLARAYTIFASGGELKPISLLKRHSSVLGQRVLS
                     IETANAMSGMLEAAVQTKGTGLKAQVSGYRVAGKTGTAHKRLENKKGYAADRYISTFA
                     GYAPVSNPRLVVAVMIDEPSAGQYFGGAVAAPLFSEVMAAALWQLNVPRDAPGNIVSS
                     PLPVKFKEEG"
     gene            complement(7314..7589)
                     /gene="ftsL"
                     /locus_tag="PKZ49_06510"
     CDS             complement(7314..7589)
                     /gene="ftsL"
                     /locus_tag="PKZ49_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HND36250.1"
                     /translation="MIKLNILLVLILIFSGLGVVTAQHETRKLVISLEKEHNLTDQLA
                     IERNQLLIEQRTWAMPGYVEKIAKNKLDMIAPSSSHITVITPDHVLN"
     gene            complement(7591..8538)
                     /gene="rsmH"
                     /locus_tag="PKZ49_06515"
     CDS             complement(7591..8538)
                     /gene="rsmH"
                     /locus_tag="PKZ49_06515"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HND36251.1"
                     /translation="MHLPVLLNEAINALAIKEDGVYVDGTFGYGGHSQSILQHLGQRG
                     RLVAFDKDLSAIDRAQSINDDRFQAIHSSFAKMQEVLHELRIERVDGILLDLGVSSLQ
                     IDEGARGFSFRSNGPLDMRMNTNIGQTAEQWLQSVTETQLEEVIRNYGEERHARKIAR
                     AVVMARAKYPISTTFQLAEIITEVMRTGGYQHGGKQHPATRTFQAIRIHLNQELEELS
                     LVLPQCVELLNPRGRLVVISFHSLEDRIVKRFMRAKANPEGNLPRKIPLTSKEVQELI
                     HREVLLIGDALKPTSIEIARNPRARSAVMRVAEKLKNGR"
     gene            complement(8550..8999)
                     /gene="mraZ"
                     /locus_tag="PKZ49_06520"
     CDS             complement(8550..8999)
                     /gene="mraZ"
                     /locus_tag="PKZ49_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HND36252.1"
                     /translation="MFRGVTPLSLDSKGRFAVPAKYHDVLKSSCDNRLVITVDSPKCL
                     LIYPQPVWEPIEKRLLTGLSSFNPKIRAMQRLLVGNACDVAMDNAGRLLVVAQLRQFV
                     GLVKDVVLVGQGEKFELWDAEKWNQEIETALEFKDGDMPPELEGFSL"
     gene            9265..9909
                     /locus_tag="PKZ49_06525"
     CDS             9265..9909
                     /locus_tag="PKZ49_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HND36253.1"
                     /translation="MAIKTTNPPIIPSYWETALKELRERDRILGKLISTDGSEALCSQ
                     GDAFTTLARAIVGQQISVKAAASVWHKLTLQLTEISPYTVFSADTDLLKTCGLSARKV
                     SYIQDLSAKFLEGIHDEAEWQRMDDESLIKLLIQFRGIGRWTAEMFLIFYMLRPNVLP
                     LDDIGLQRAISIHYNQNQTIDKQSIQKIAEEWQPWRSVATWYLWRSLDPIPVAY"
     gene            10317..11300
                     /locus_tag="PKZ49_06530"
     CDS             10317..11300
                     /locus_tag="PKZ49_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36254.1"
                     /translation="MQTKMGTAKKSKSMYDVQTYSNSSLVKKLYFNQLAVPEKTSKAT
                     HIPSEQRTSKLESDSDNCLLRRNGYFIREISSLKQRLKVNSLIKRRYASRGYNTDQTT
                     VFSADKDQITFEACNESGQLGTLTLIIDLGKGLLADQHYQEEIDAFRGQAKKVCEVSK
                     LAFNPSSSSKEIFASLFHIAYLYAYCIYGVKNAFIEINPRHAAFYKRMLGFEQIGEER
                     VCKRVDAPAVLLRLDLEYMREKIIRNQATSGSNNKSIYGYFLNQNEEHAILSIIQNLP
                     AQQKKSGQEKLSAAIPLAALNTGNTSTLLYNLFTEAIKENRAIGLEKLVYC"
     gene            complement(11410..>11603)
                     /locus_tag="PKZ49_06535"
     CDS             complement(11410..>11603)
                     /locus_tag="PKZ49_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HND36255.1"
                     /translation="RLNQFDPEVVNNSCFNGTAGQGDWCLGGTADIAGGTVTVPEPAT
                     MALLGLGLVGMGLGRRFAR"
BASE COUNT         3436 a         2810 c         2079 g         3278 t
ORIGIN      
        1 ataataatcc gatgtggctg caaatttttg tcgattaatg gttggttgga caatcatagg
       61 ctagggctgc tctcaactaa tgaaatacct ttactctcta attctttaac cgccacagta
      121 aaaacctcgg cgcgatgaat ataatttcta aacatatcaa gacttgcgca agccggagaa
      181 agcaaaacca tatcgttcgg tttggccaat aaaaagctca accgcaccgc atcttccatc
      241 gtcgatacat gatgaatcgg tttagcagct cctttgatag cctggcttat tttttctgcg
      301 tctctcccca gtagtacaag cgctcgaacg tattgtttaa tgggttcaat taaaggtgag
      361 aaatcctgcc ctttcccatc ccctcccgca attaacacaa tattgccttt catcccattt
      421 aatgctgcga tggcagaacc gacattggta cttttagaat catcgtaata atcaacccct
      481 tgtatggttg caattttctg catccgatga ggcaatcctc taaatttcag taaagcatcg
      541 actaaaggtt catgcggtaa ccctaccgaa cgacacagtg ccaatgctgc cagcgcatta
      601 gccacattat gcatgccagg aatttttagc atatctgctt ttagaagccg cctttttccc
      661 tcaactaacc ataaagcaga accaaactcc agcaaaccaa aatcgacgtc tcggcttggt
      721 ttactcaaac cgaaagtcac ttgcttttta tcaggcaacg ccattgcaag cacgtttgga
      781 tcatcccgat taagaatctg cgtactatga gtgccgccaa taaaaattct tgcttttgca
      841 gcagcatagt catctaggcc atcatagcga tcaaaatggt cttcgctaat attcagtaca
      901 actgcgctat tagcaaaaag tgtttgggtc gtttcaagtt ggaagctgga taattcaagt
      961 acccagcatt gtggtagtcc tttggtagaa agtcgctgca gtaagacttt taatacagca
     1021 ggcccaatgt ttccggcgac ttccacatcc catccagccg cttttaccat gtcacccacc
     1081 atgctcgtta cagtcgtttt tccattggag cctgtaatgg caaggataat cggtttcggt
     1141 tgatttagct ggttcagtgt tagagcaaat aactcaatat caccgaatac agggatatta
     1201 cgcttaatgg cttcttgcac aaaagcttct gctagcggaa ctccagggct aattgcgatc
     1261 gcatcaatat tttctagtaa gttcaagtca aaatgaccaa aaaacctttt ctggtgaggg
     1321 ataaaatcat ccaagaaatt tggattgggt gggttagcac gactatcggc aagcgataag
     1381 tgcgctccta aatatgaaag ccatttaacc atggagattc ctgtctcacc cgaccccaga
     1441 actagaacct ttttttcatg gaaaatcatc gtaatttaag cgtcgagagc cctaataaaa
     1501 ctaaaattaa agtaataatc caaaatcgaa cgacaacctg attctccttc caccctttca
     1561 attcaaaatg atggtgaatc ggggccatac gaaaaattct tttacctcgg agcttaaaag
     1621 aggctacttg tagcatgact gacatagctt ctgcaacaaa caccccgccc ataatcacta
     1681 aaacaatctc ttggcgaaca ataactgtaa tcacacctag cgcggctcca agggataacg
     1741 ctcccacatc gcccataaag acttcagctg ggtaagtgtt aaaccaaaga aaagccaggc
     1801 cagcaccagc taatgctcca cagaaaactg caagctcgcc cacattctga atataaggaa
     1861 tacccaaata tttcgcaaaa acgatatgcc ctgttacgta agcaaagata gccaatgcac
     1921 cactgatcat gacagttggc ataatagcca agccgtccaa tccatctgtc aaattaactg
     1981 cgttactcgt tccaacaata acaagataag tgagcacgat aaaagtgacc attcctaaag
     2041 gaatcgcaac ctccttaaaa aagggaacga tcatattggt ttgctcagga gtgtctgcaa
     2101 tccaataaag ataggctgca acgcagcacg cgattacgga ttgccagaaa aatttagctg
     2161 cggccgaaag acctttagaa ttgcgatgca cgaccttccg ataatcatcc acccaaccaa
     2221 tgacaccgaa tcccaatgtc gtcagtaaaa cgacccagat gtaagggttc gttaaatccg
     2281 cccatagtaa tatcgtaatg acgatcgacg tcaaaatcaa agcaccgccc attgttggag
     2341 tgcctgcttt aaccaaatga gactggggcc cgtcttcacg aattgattgc ccaatcttgt
     2401 aggctgttaa tttacgtatc attgccggtc caataacaaa agatatcacc aatgctgtca
     2461 atacagccaa taccgtccgt aatgttatat agttgaaaac attaaaaaca cgaacatctt
     2521 gtgcaatcca ttgggcaatg agtaacagca tttttatcct tatgtgatct ccgttttgat
     2581 cacgcgtatt taacctttgt taagcaagtc aatgtcgcac ttcaaatcgc tgaataactc
     2641 gctccatttt cataaagcga gaacccttaa ctagcattgt gacattgtga tccaataatt
     2701 tttcagcttc ttctaataac tcatcgatat tggaaaaatg catggctcct tttccaaacc
     2761 cttctgccgc attcacactc aattcaccca gcgcaagtaa tttttcaata cctgctcggc
     2821 ttgcttcctc accgattttc cgatgaagat cagcaccccc aacaccaagc tcacccatat
     2881 cacccaatac gaatattttt cttccagata aatgtgctaa aactgaaagt gctgcactaa
     2941 cggatgcggg atttgcatta taagtatcat caatcaaagt tgcttgatgc aatccacttt
     3001 ttttctgcat acgacctttc acgccactaa atgccgacaa accttcaata ataacttgct
     3061 cactgactcc agcacctaac gctgcagcag ctgcagccaa tgcattcatt acattatgtt
     3121 gtccgagtac ttgtagttcg atctcacttt cttcctttgg aaattttaag atccacttat
     3181 tactcgatgc tagcaggtgg caatccgctc taatctgagc ttttgcattc attccaaaac
     3241 ttattatctg gcgattacct gctagttgat accaaagctg tgcatatttg tcatctgcat
     3301 taatgatggc tacgcctggc ttatctaagc cctgaaaaat ttcgcccttt gcatgcgcaa
     3361 ccaactcaat cgaacccaac ccttcgatat gggcggcacc ggcattggta atcactgcaa
     3421 tatcgggttt tgctaactgc gttaaataag caatttctcc ggcatgattc atccccattt
     3481 ctactactgc ataaacgtga tgttgtcgaa gtcttagtaa agtaagtgga acgccaatat
     3541 cattgttaag attaccgatt gtcgctagca cctgatcaac tgaagcatcg gtagtaaaat
     3601 tagattgacg ggaagccgcc ttagtaaaaa tagacgcaat catctctttg actgtcgttt
     3661 ttccattact tcctgtcaca gccaccaatg ggagtgagaa ccgatcacgc cattgagacg
     3721 ctaattgccc tagcgctaat cgagtattag caacgtgtat ccatccaaaa tgttgcggta
     3781 atttggtttt tttaatctct cttgcaacta gggctgcaac ggcacctcgc tcgattgtgt
     3841 caccaatata atcatgtcca tcaaatcgat cacccgataa tgccacaaac aaatcacctg
     3901 ctttaatttg acggctgtca gtactgacat caccaaacac gaggtcatcg ccatgccaat
     3961 cagcattcag catgcgagca gcctcttgaa cagtcatcat ctgcgtactc gcattttcat
     4021 attcgctaaa tcgtgaagta cttgttgggt aacttcacgg tcgtcaaatg gcaatcgctt
     4081 acctttaatc tcttggtacg cttcagcgcc ttttccagca attagcacaa tatcgttctt
     4141 atgtgcatta tgaatcgcct gataaattgc agaagcacga tcttcttcga ttatataatt
     4201 ctggtatcga gtaccggcca cgatctcatt gataatatcg cgcgcatcct cgttacgggg
     4261 attatcactg gttacgataa cctgatccgc taaacgcgtt acgatacccc cagcaatcgg
     4321 tctcttaccc cgatctcgat ccccaccgca tccaaataca cagatcaatc tagcttttct
     4381 agaatgaggt ctttgtttta aatgactatt cactaatgtc tcacgcaacc cagttaacac
     4441 tttttcaagg gcatccggtg tatgggcata atcgacaatc accgttggct gagctccccc
     4501 accaaatttt tccattctac ctgggacggg ctttaaacgt gacaggactg caaccgcatc
     4561 ctgcaaatta acaccgctcg ctatgaggga agcaatgact gctaatagat tattggcatt
     4621 aaatcgactg aataatgtag tcttgatatg cgctttctcg tgctcaaatt caacatcaaa
     4681 ttccagacca tcaatggtcg cttgtaaatt attgccacta agtactcgca gatttccttg
     4741 atttagtttt aaatcgattg attggttaaa accataaccg ataatttgca gatccctttt
     4801 tccaatctgc tgaacgagtt caataccaaa aacatcatcg aggttcaata ctgcacattt
     4861 gagatttggc cagaaaaata gtttggcttt tgccgcagca tactcatcca tatttttatg
     4921 gtaatccaag tgatctcgcg ataaattagt cagcacagcc actgcgaact cgcaaccatt
     4981 gacacgccct tgaacgagac catgagaaga tacctcgatc gctaaatttt gtgcaccttc
     5041 ttttaaacaa ctcgctagcg tctcctgcag caaaacagca tcaggcgttg tattagcagt
     5101 atatttcaga tcctgtagga aaccattccc taacgtacca attactgctg tttttttccc
     5161 aagcatcgtc attgcctcag cataccagtg actacacgat gtttttccat tagttccagt
     5221 aaaaccaacg agccacaggt gacgcgaagg gttcccatac actcgacttg agatcaaccc
     5281 tgccttttct ttgagatcta ataccgcaca atttgggata acccactctg gattccatga
     5341 aaattgctgc ttctcccaaa ttacagcatt tgcacctgca gcaatcactt tagctatatc
     5401 agaacgcgca tcataacgat cccccgcata agctagaaaa gtatttcctg ctttaatgtg
     5461 acgataatca gttactagtc gcttaattgg cacattgagc tcagcaagaa tgcgatctac
     5521 aagaagctca tcggacttcc atgagattaa agaactcatc cctcctcctt aaatttcact
     5581 ggcaaaggag aactcactat atttcctggt gcatcacgag gaacattgag ttgccataaa
     5641 gcggctgcca tcacctcact gaagagtggt gcagcaacgg cacctccaaa atattggccg
     5701 gcagatggtt catcgatcat gacagcaacg actaatcttg gattagatac cggcgcatag
     5761 cctgcaaacg tcgaaatata tcgatctgca gcatagcctt ttttgttttc gagacgctta
     5821 tgcgcagtcc ccgtcttacc tgctactcga taaccgctca cctgagcttt taaaccggtt
     5881 ccttttgttt gtactgcggc ctctagcatg ccactcatcg catttgccgt ttcaatggac
     5941 aaaacacgct gaccaagcac tgaagaatgc cttttaagca aagatatcgg ttttaattca
     6001 cctcctgatg caaaaatagt ataggcgcga gcaagctgca ttaaattgac cgaaataccg
     6061 tgaccatagg acattgtaac ttgctcaatc ggcttccatt tctgatatgg ccgcaatacc
     6121 ccattgacct cccctggaaa tccagatccg gttgaagtac caaaaccaga ttgactaagc
     6181 atttcccaaa atgtcttggg tggaagcatc aatgcaattt ttgcagttcc tacattactt
     6241 gactcttgaa tcacttgtga taccgataac gcccctttat ttcggacatc ccgaatcgtc
     6301 gctttcccta ccttaagaaa accaggtgct gtttctaata gcgaagtcgg tttgattttg
     6361 ccggtctcca tggcaatgga tactgcaaaa ggcttcaaag ttgatcccgg ctcgaatgta
     6421 tcgacgaatg cacggttacg taagctttcc ttgcggatag aagaacgttg attaggatta
     6481 taggtagggt aattagcgag cgctaacact tcgccggttt gtgcatccag tataatcaca
     6541 ctccctgctt tagcacggtg ggattctacc gcttgtttta attctcgatg tgcaagatat
     6601 tgaattttac tgtcaatcga taagactaaa ttttgaccag gctgaggtga tcgaattctt
     6661 tcaacatttt ccacaactcg accgatgcga tctttaatga cgcgcctgct gccatgcttc
     6721 cctgtcaaaa ttccttccca acccagctca actccttcct gcccttttcc atcgatatct
     6781 gtaaaaccga ggacatgagc tgccatctca cctgtcggat aatatcgata aaattctctt
     6841 ttgagataga gacctttaat gtttaagtcc gcgatatttt ctgcagtttc agggggtaat
     6901 tggcgtttca aataaacgaa gcgtttatcc ttatctgaaa ttcgtttatg aatctctgca
     6961 acatcaactt ctaatatttg agctaacttt attaattgat cgggagtcac attaagctgt
     7021 tggggatcga cccatacgga ttttacaggc gcactaattg ctaaaatctc accattacga
     7081 tcagtaattt ttccacgctg tgcgtttaat tctacgactc gactgtacct cgactccccc
     7141 tgctgcttta aaaagtcttt atttaagctc tgtaaataga ttgcacgtcc tgatagggct
     7201 attaagctca ataaaagcac aatgatcaga aaccgtgaac gccaaactgg taaataaatt
     7261 gctgtagccg ctgtttgata tctcatcact aatcacccat cgattggctt agctcaattt
     7321 aaaacatgat cgggagtaat aaccgtaata tggctcgatg aaggcgcaat catatcgagt
     7381 ttatttttgg caattttttc aacataacca ggcattgccc atgtgcgctg ctcaattaac
     7441 aattgattac gctcgattgc aagctgatca gttaagttgt gctctttctc aagtgaaatg
     7501 acaagctttc tcgtctcatg ctgcgctgtc acgacaccta gccccgaaaa aatgagtatc
     7561 aataccagca aaatgttgag cttaatcatt tcacctcccg ttctttaatt tttccgcaac
     7621 acgcataaca gcacttctag cacgcggatt tcttgcaatc tcaatgcttg taggctttag
     7681 ggcatcgcct atcaacagca cctctcgatg aatcaattct tgaacttcct ttgaagtcaa
     7741 cggaatcttt cttggcaaat taccctctgg atttgctttt gctcgcataa aacgcttcac
     7801 aattctgtct tccaaggaat gaaaactaat cactaccaac cttcctctgg gattcagcag
     7861 ctcaacacat tgcggtaata ctaacgaaag ctcctcaagt tcttggttga gatgaatcct
     7921 aattgcctga aaggtccgtg tcgccggatg ctgcttaccc ccatgctgat acccacccgt
     7981 gcgcatgacc tctgtaataa tttcggcaag ctgaaacgtg gtagatatcg ggtatttcgc
     8041 tcttgccata acaactgctc ttgcaatctt ccgagcatgc cgttcttcgc cataatttct
     8101 gattacctct tctaattgtg tttcggttac cgattgcagc cactgttctg cggtttgccc
     8161 aatattcgta ttcatgcgca tatctagggg cccattactc cggaaactaa atccacgcgc
     8221 tccttcatcg atctgtaacg atgaaacccc taaatctaat aaaatcccat caacccgctc
     8281 gattcttagc tcatgcaaaa cttcctgcat ttttgcaaag ctgctatgga tagcctgaaa
     8341 tcgatcatca ttgattgatt gtgctctatc gatagcgctt aaatccttat caaacgcgac
     8401 caatcttcct ctctggccta gatgctgaag aattgactgg ctatgccccc cgtaaccaaa
     8461 agtaccatcc acatagacgc catcttcctt aattgccagc gcgttaatcg cttcgttaag
     8521 caatactggc aaatgcacag aggcaccgct tacaaagaaa aaccttctaa ctctggtggc
     8581 atatcgccat ctttaaactc aagcgctgtt tcaatttctt gattccattt ctcagcatcc
     8641 catagctcga atttttctcc ctgccctact agcacaacat cttttactaa cccaacaaat
     8701 tgtcttagct gcgcaaccac caatagtctt cctgcattat ccattgccac atcacacgca
     8761 tttcccacaa gcagtcgctg catagcccgg atcttggggt taaaacttga aaggcctgtc
     8821 aatagccgtt tttcaatagg ttcccatact ggctggggat aaatgagtag gcatttggga
     8881 gaatcgactg ttatgactaa ccgattgtca cagcttgatt tcagtacatc atgatatttc
     8941 gctggaacag caaatcttcc tttgctatca agactaagcg gtgtaacgcc acgaaacata
     9001 tcactccatt tttatactca tcgcccacat tgatctaatt ttaccccaca atctaccaca
     9061 taattaccct aataacccac ttttatccac tctaattaaa aaaaaatact acgtcaagca
     9121 aaaacaatta atcttttcaa tctacacaat gagttacaga cgccacaccc aatttgattt
     9181 acgcagagaa ggtataaaaa gtaaaattac caatgacact cacagcactt cgcgcctggg
     9241 aagaaacctt taatcagaat tgacatggca attaaaacaa ctaatccacc aatcatccct
     9301 agttactggg aaaccgcact gaaagaattg agagaacgcg ataggattct tggtaagctg
     9361 atttcaaccg atggaagcga agcgctgtgc tctcaaggtg atgcgtttac aaccttagct
     9421 agagcgattg tggggcagca aatctctgta aaagcagcgg ccagcgtttg gcataagttg
     9481 acattacaat taactgagat ctcgccatat acggtatttt ctgcggatac agatttgcta
     9541 aaaacctgcg gcctttctgc aagaaaagta agctatattc aggatttatc ggcaaaattt
     9601 ctcgaaggca tccatgatga agctgagtgg caacgcatgg atgatgaatc gctcatcaaa
     9661 ctacttatcc aattcagagg gatcggaaga tggaccgctg aaatgttttt aatattttac
     9721 atgctacgcc ccaatgtgtt gcctttagat gatatcggat tacagcgcgc tatcagtatt
     9781 cactataacc agaatcaaac aatcgataaa caatcaattc aaaagattgc tgaggaatgg
     9841 caaccttggc ggtctgtcgc cacctggtat ctctggagaa gcctagatcc catcccagtc
     9901 gcttactaac cgactcaatt cctctcagag caagtaaaga attttctctc tgcaattctt
     9961 attttcaatc acacaaaatt gaaaaaacaa ccgtctcaat ttagagacaa aatttaatca
    10021 atttaatcat atggttatca atatcaccca aataatgcgt taccttagag cgacacaatc
    10081 aagctagaca gaaattcacc aaatcattca acccattgaa tattatcaat tatttttggt
    10141 ggcacgctta ttgcgtaagt caaagataca gcgcttctta cgagagctta catagaataa
    10201 aagactcgtt ttataagcat ctaggctgac agtaaagata tagaaacttc tgaaatcaat
    10261 cagagctaag ctttacattc ataggccatt tggctacaag aaaagaggaa tggattatgc
    10321 aaacaaagat gggaaccgcg aaaaaatcta aatcgatgta tgatgtacaa acatactcaa
    10381 acagttcatt agtcaaaaaa ctatacttca atcaactggc tgttccagaa aaaacgagca
    10441 aagcaacgca tattccatct gagcaaagaa cctctaaatt agaatccgat tctgacaact
    10501 gtctactcag gaggaatggt tattttatta gagaaatctc atcacttaag caacgcctga
    10561 aggttaactc tctcatcaag cggagatatg cctctcgtgg atataatact gaccaaacaa
    10621 cggtattctc agcggataaa gatcaaataa cctttgaagc atgtaacgaa agtggtcagc
    10681 taggaacgct aacgcttatc attgatttag gcaaagggtt acttgctgat cagcactatc
    10741 aggaagaaat tgatgctttc cgtggccaag caaaaaaagt atgcgaagta tccaaacttg
    10801 cttttaatcc cagctcaagt tctaaagaga tttttgcttc attgtttcat atcgcttatt
    10861 tatatgctta ctgtatttac ggcgtaaaga acgcattcat cgaaatcaat cctcgccacg
    10921 cggcctttta taagagaatg ttaggatttg agcaaatcgg tgaagagcga gtgtgtaaac
    10981 gtgttgatgc tcccgctgta ttgttacgct tagacttgga atatatgcga gaaaaaatta
    11041 ttagaaacca agcaacatcg ggttctaata ataaatctat ttatggttac tttctaaacc
    11101 agaatgaaga gcacgctata ctatcaatca ttcagaattt accagcgcag caaaagaaat
    11161 cagggcaaga aaaattatcc gcagcaatac cgcttgcagc attaaatacc ggaaatacaa
    11221 gcactttact ttataactta tttactgaag ccatcaaaga aaatcgagca ataggtttag
    11281 agaaactcgt atattgctaa atctcaatct agatgctttt attaggaatc tagaagtcct
    11341 cactacataa agagcggact tctagataat caattgcaag ttactttatt tttcagtttc
    11401 ttttacttct tatcgggcaa atcttcttcc caaccccatt cctactaaac ccaatcctaa
    11461 taatgccatt gttgctggtt ctggaactgt aaccgttcca cctgcaatat cagccgtacc
    11521 tcccaaacac caatctcctt gacctgctgt accattaaag cagctattgt ttactacttc
    11581 cggatcaaat tgattcaaac gac
//
LOCUS       DAOQKQ010000077        11485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_2640_length_11485_cov_5.548644, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000077 DAOQKQ010000000
VERSION     DAOQKQ010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 11485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11485
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2640_length_11485_cov_5.548644"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="PKZ49_06540"
     CDS             <1..533
                     /locus_tag="PKZ49_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HND36256.1"
                     /translation="ALRAFTPRDQKAVKSAAQTFRANPNIDIETAITELGVGEALISF
                     LDEKGRPNPVEIGKVLPPASRVGPATESEVAQIINASAFYGYYENAIDRESVYEILKV
                     RGDSRAQHAEKSDESHGFDWRSVLGSSSSTKSSGRQTQGIIETVAKSMARTVGNEVGR
                     QILRGVLGSILGKGRR"
     gene            complement(551..1384)
                     /locus_tag="PKZ49_06545"
     CDS             complement(551..1384)
                     /locus_tag="PKZ49_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36257.1"
                     /translation="MSSLLSNVVSLQGVTQSRLVAIVILVSFILGACVQPQPKPDLAT
                     QTTANPSQPTELEPIPIPEKLPCAKQCEAEINRLKKQLKEKGDLLRSYQAQLKDQKPP
                     NSENVSEATQAQTKLHRLASQPSAASKIAEVEAETENLKQSLLINADRSDQRLFNHSQ
                     QLINAASQAYQKQDYAASMNFATQSQAIIEMIKNTARKLTAQSTIFPLKTPIIVLVQK
                     NSPIFAEPANTAAPLEKLQKDTTFTTNAYLANWLRVQTPSGQIGWIDSMHTQINIPNS
                     N"
     gene            complement(1457..2305)
                     /gene="ccsA"
                     /locus_tag="PKZ49_06550"
     CDS             complement(1457..2305)
                     /gene="ccsA"
                     /locus_tag="PKZ49_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HND36258.1"
                     /translation="MTGTLVYILAFLFYTFAGWYFWQTRWKHQSIYPVNQGNDPSRAN
                     WQAHLIMLVPIIFHGLSLSQSIFIETGLSFGVGNAISSIVWLTGLIYWFTGFFPRLQG
                     LQNLFAPIAIAGAIAVLLPLLIPSVRPLANTEMTAFKMHLLVAMLAYSLFTIAALHAL
                     LMTIQEYRLHHPTLSPLLTNLPPLLAMEKMLFHIIWTGFTLLTLTLFSGIIFSQEVFG
                     RSITFSHKTLFGFISWGVFAALLAGRKLYGWRGHTAIRWTLVGFTILLLAYIGSKFVL
                     EIILNR"
     gene            2419..3765
                     /gene="ffh"
                     /locus_tag="PKZ49_06555"
     CDS             2419..3765
                     /gene="ffh"
                     /locus_tag="PKZ49_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HND36259.1"
                     /translation="MFENLTERLSGVIKTLRGEARLTEENIQAALREVRMALIEADVA
                     LPAIKDFIERVKQKAVGQDVLASLSPGQALIGVVHQELIAIMGGDKAELNLSTSPPAI
                     ILMAGLQGAGKTTSSGKLAKWLIDKKKKVLLVSCDIYRPAAIDQLELLAKQVGADFFP
                     VQTGQKPKEICSAALDYSRRNHHDVIIVDTAGRLGIDEFMMNEISELEALLKPIETFF
                     VVDAMQGQDAVNTAKAFADALPLTGVILTKLDGDMRGGAALSVRHVTGKPIKFVGIAE
                     KLTGLEPFFPDRMASRILGMGDVLGLIEDAQRSSDQKEAEQLMKKMKSGKGFDLNDFK
                     MQFQQMKKMGGMTAMLEKLPHQLSQAAQNVKVDDRIINRNEGIINSMTQLERTKPELI
                     KASRKRRIAAGSGVSVQEVNRLLAQFEQAQKMMKMVNKGGMAKMMRAMKGILPQFR"
     gene            complement(3793..4242)
                     /locus_tag="PKZ49_06560"
     CDS             complement(3793..4242)
                     /locus_tag="PKZ49_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HND36260.1"
                     /translation="MIRSNLFILSFCLGLTLSPLLLAENLVVSLTIGGHPLNAEIAHT
                     YDTRSKGLMDRATLPENQGMLFIFPKIDFYSMWMLNTSLPLSVAFIDEKGIILNIEAM
                     EPFSTVAHASIKPAKYALEMHQAWFTSRKITAGHKIIGLENVPDAIE"
     gene            complement(4239..4682)
                     /gene="dut"
                     /locus_tag="PKZ49_06565"
     CDS             complement(4239..4682)
                     /gene="dut"
                     /locus_tag="PKZ49_06565"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HND36261.1"
                     /translation="MKIDIKILDTRLQTQLPSYATPGSAGLDLRACTEQPLTVHPGET
                     QLIPTGIAIHIEDPGFAAVVLPRSGLGHKHGIVLGNLVGLIDSDYQGQIFVSCWNRGQ
                     TEFLLNPLERIAQLVVIPVVQAQFNIVENFAMSHRGEGGFGSTGR"
     gene            complement(4778..5839)
                     /locus_tag="PKZ49_06570"
     CDS             complement(4778..5839)
                     /locus_tag="PKZ49_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HND36262.1"
                     /translation="MRVILCLVLIVSSYAYAQSGHDHQHKSQKEDTQQGGMQGHGDGH
                     HGMGGMMMHGGHHKLDGATLKHFFEPLPASIIDEKKNATLIALGKKLYLDPRLSINDT
                     ISCNSCHQLNNFGVDSQPTSPGHDGTRGGRNSPTTFNAALHIAQFWDGRAKDVEEQAL
                     GPILNPIEMGMPNEEAVVNKLKKIDEYKTLFANAFKDEKDPIQYKNIGKAIGAFERTL
                     LTPSRFDDFLKGDENALNDAEKRGLTKFVHMGCANCHNGVAIGGNSYKKIGLVEPYET
                     KDLGRYEVTGLETDKKVFKVPSLRNIAQTGPYFHDGSIATLEQAIEEMAEHQLGREVG
                     PGFIEDVKAFLGSLTSKSK"
     gene            complement(6066..6647)
                     /locus_tag="PKZ49_06575"
     CDS             complement(6066..6647)
                     /locus_tag="PKZ49_06575"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HND36263.1"
                     /translation="MLKLLMQYFYNLGSLFSHKKPSAILYLLLIGISAILPKPLQAEE
                     VSEYGLKALFLYNFAIFTSWPGSNIDKFNLCIYGDNPFGQDINFLMKSKKINERTIDI
                     HRIDNVSQLDQCQLVFISRPMISNLADVITTVKDKPILTIADSPGACHQGVILNMNVN
                     NEKVTFEANLMMAKKAGLNLSSQLLRLATEIYQ"
     gene            complement(6668..8659)
                     /locus_tag="PKZ49_06580"
     CDS             complement(6668..8659)
                     /locus_tag="PKZ49_06580"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HND36264.1"
                     /translation="MVVTSYRKPICASKTHWQLSLFLLLAPFINSAFAAQNIQTIRNL
                     ADASLEELMNVKISTVFRKPQTLQETAAAVFVISQEDIRRSSANSIPELLRMAPGLSV
                     ARIDSNKWSVTSRGYSGRFADDLLVMIDGRTVYSPLFAGTFWETLDLPLEDIDRIEVI
                     RGPGGTIWGANAVNGAINIITKHTKATQGNLITTGGGSEERGFATFRHGGRVGENLFY
                     RFYAKGFTRDNSFNASGTNDSWRMGTLGFRADWDINTNNAVTFQAQYYAGKAGQNTML
                     LNPTEPESAITRPEDANLAGGHALMRWQRTTGERSNTALQVYYDRTSRNELSFRETRQ
                     TVDIDFQHRFPLQIPYIPFQPDLIWGGGYRWTTDHLGINLPISFDPAKRNLQTGNVFM
                     QTDIPLFEDRLKLTTGIKFLDNTYAHSNFLPNARLLWTPDDKQSVWLSATRSIRLPSR
                     FERDGNQLIRSGAEFIRLISNPHLLAETLWGFETGYRRQVTSNLSVDIAGFFNDYNNS
                     TSERETTAETIQLTNQRKTQIFGFEIYGEWRAFDKLRFMPSYSHLQVRNHVPADHESE
                     SGEDPKHQFTLRTQLDLTKNIELDAFFRHIDKLPGLEIKSYQALDLRLSWRPRSDTEL
                     TLVGQNLLQSHHQEFSPEVIQTMPVQIQRGVFGKVTWRF"
     gene            complement(8853..10058)
                     /gene="coaBC"
                     /locus_tag="PKZ49_06585"
     CDS             complement(8853..10058)
                     /gene="coaBC"
                     /locus_tag="PKZ49_06585"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HND36265.1"
                     /translation="MDSISALTGKKLILGITGGIAAYKTAELARLLVKEGAIIQTVMT
                     QAACRFIGPETLHALTGNPVYTELWGDSLQNSMAHIGLSRSSDAILIAPTSANFIAKL
                     AHGVADDLLSTLCLARDCPLIIAPAMNKQMFENPATQRNLNTLHADQITIWGPASGDQ
                     ACGENGLGRMLEPEQLQQALQVFFEPKWLDGKNVLITAGPTYEAIDAVRGIINLSSGK
                     MGYAIANAASNAGATVTLIAGPVHLPKPSVQKFISVTSATQMLAAVKKEVSNCDIFIS
                     VAAVADYRPQTYQPQKIKKANQPLTLELVPNPDILHYVAHLKKPPFCVGFAAETEKLD
                     QYAQKKRQQKRLPLIVANLAQQAMGADESTLILFDDNGKHVLPQASKTELARQLIKHI
                     AQLFEKQNA"
     gene            10207..10881
                     /gene="radC"
                     /locus_tag="PKZ49_06590"
     CDS             10207..10881
                     /gene="radC"
                     /locus_tag="PKZ49_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HND36266.1"
                     /translation="MAISDWPELERPREKLLHYGPASLSDSELLAIFLRTGITGKSAV
                     DLARELLSHFGSLTELFSADQRSFCQIPGLGVAKYVQLKATLEMAQRALETGLKRGDI
                     MTSPQQVRQFLRLSLVHRQHEVFMGLFLDARNRIIQSEELFSGTLTETSVYAREVIKR
                     ALFHNAAAIIFAHNHPSGHAEPSQADEVLTQSLKQALAYVEVKVLDHFIVGSEGITSF
                     AERGLL"
     gene            complement(10884..>11485)
                     /locus_tag="PKZ49_06595"
     CDS             complement(10884..>11485)
                     /locus_tag="PKZ49_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HND36267.1"
                     /translation="HPDYENITEPTVAQLVELAQHPKVIAVGETGLDYFRIKGDLDWQ
                     RDRFRRHIRAARESQKPLIIHTREAAEDTLRIMQEEDAATIGGVMHCFTENWDVAQRA
                     IEMNFYISFSGIVTFKNALMLKEVARKVPLNRMLIETDSPYLAPVPHRGKTNQPAFVK
                     HVAEEIAKLREKSLDEIATATTDNFNTLFRLPHTSLTTH"
BASE COUNT         3224 a         2269 c         2663 g         3329 t
ORIGIN      
        1 atgcattacg tgctttcacc cctcgcgatc aaaaggcagt taaatcggca gcgcaaacat
       61 tccgggcaaa tccgaatatc gatattgaaa cagcgattac agagcttggc gtaggggaag
      121 cgttgatttc atttttagat gaaaaagggc gccctaatcc ggtcgagatt ggtaaagtat
      181 taccgcctgc tagtagagta gggcccgcaa ctgaatctga agtagcccaa attattaacg
      241 catcagcatt ttatggctat tatgaaaatg cgattgatcg ggaatcagta tatgagatcc
      301 ttaaagtacg gggcgatagt cgcgcacaac acgctgagaa atcggatgaa agccatgggt
      361 tcgattggcg ttcggttttg ggtagttctt caagtactaa gtcatccgga cgacagactc
      421 aaggtatcat agaaacggtt gccaaaagta tggccaggac cgttggtaat gaggtaggtc
      481 ggcaaatttt gcgtggtgta ttgggatcga ttttaggtaa aggacgccgt tgagtaagcc
      541 aatttcattc ctagttcgag ttgggaatat taatttgagt atgcatgctg tcaatccagc
      601 cgatttgtcc tgacggtgtt tgcactcgta accaatttgc caaataggca ttcgttgtaa
      661 aagtagtatc cttttgtagt ttttctaatg gtgctgccgt gttagctggt tctgcaaaga
      721 ttgggctatt tttttgtacg agcacgataa tcggtgtctt aagtggaaag atggtcgatt
      781 gagcggtgag tttacgtgct gtatttttaa tcatttcaat aatggcttgc gattgagtcg
      841 caaaattcat ggatgctgca tagtcttgtt tttggtaagc ttgagaggcc gcattgatta
      901 actgttgaga atgattaaat agtcgctgat ccgatcgatc tgcattgatt aacaaagatt
      961 gtttgagatt ttcagtttcc gcttctactt cggcaatttt tgaagcagca ctcggttgtg
     1021 aagcaagtcg atgtagttta gtttgagcct gcgtggcttc actgacattt tcactattgg
     1081 gtggtttctg atcttttaat tgtgcttgat agctacgcag caaatcacct ttttctttaa
     1141 gttgtttttt cagtcgattg atttcagctt cgcattgttt ggcgcacggt aatttttctg
     1201 gaataggtat cggttccaac tcagttggtt gactaggatt agcggttgtt tgggttgcta
     1261 aatctggttt tggttggggt tgaacacagg cacctaagat aaaactcacc aaaataacga
     1321 ttgctaccaa tctagattgt gttacaccct gtaaagacac gacattgcta agtagactcg
     1381 acatgattcc aatccgatga ttaaaaattc atagcgaaat gattggaaag tttaaagcga
     1441 aatagtggaa atcatttcaa cgatttaaaa taatttctaa cacaaattta ctgccgatat
     1501 aggccagcag tagaatggta aagcctacta aggtccatcg gatagcggta tggccgcgcc
     1561 acccatacaa tttcctccca gccagtagtg cagcaaaaac tccccatgaa ataaagccaa
     1621 aaagtgtttt atgtgaaaaa gtgatcgatc gtccaaagac ttcttgtgag aaaataatgc
     1681 cactgaataa tgtgagtgtt aggagtgtaa aaccagtcca aataatgtga aatagcattt
     1741 tctccattgc gagcaacggt ggtaaattcg ttagcaacgg tgatagtgtt ggatgatgca
     1801 aacgatattc ttgtatggtc attaacagcg cgtgcagtgc agcaattgta aataagctat
     1861 aagccagcat agcaaccaac aaatgcattt taaatgcagt catttcggta ttggctaggg
     1921 gtcgtactga ggggatcaaa aggggcaata acacagcaat tgctccagca atcgcaatcg
     1981 gtgcaaatag attttgtaga ccttgtaaac gtgggaagaa gccagtaaac caataaatta
     2041 aaccagtcaa ccatacaatc gaggaaatcg cattacctac gccaaagcta aggcctgttt
     2101 caataaaaat tgattgggat aggcttaatc catggaaaat aatgggaacc aacatgatga
     2161 gatgcgcttg ccaatttgct cgcgatggat cattgccctg attgactgga taaatcgatt
     2221 gatgtttcca tcgtgtttgc cagaaatacc agcccgcaaa ggtgtaaaat aggaaagcga
     2281 ggatataaac taatgtgcca gtcattcgaa gtagagtata gtttactaac catagatttc
     2341 ggtgtgcaca gtctacaatg ggacggccta aatttaaagc gtaaaaatta ataatgaata
     2401 attatttgag agaacactat gtttgagaat cttactgaac gtttatcggg tgtcattaaa
     2461 acgttacgtg gtgaggcaag gttaaccgaa gagaacattc aagctgcttt acgggaagtg
     2521 cgcatggcct tgatcgaggc cgatgttgcg ctacctgcaa tcaaggattt tatcgaacgc
     2581 gtcaaacaaa aagcagttgg tcaagatgtt ctcgcaagcc tttcgccggg gcaagcgttg
     2641 attggtgtag tgcatcaaga attgatcgcc attatgggcg gcgacaaagc agaactcaat
     2701 cttagcacat cgccgccggc tattatttta atggccgggt tacaaggtgc aggtaaaacg
     2761 accagtagtg ggaaactcgc caaatggttg atcgataaga agaaaaaagt attgttagtc
     2821 tcatgcgata tttaccgtcc cgctgcgata gatcagttgg aattattagc taagcaggta
     2881 ggtgctgatt tttttccagt acagactgga cagaaaccca aagaaatatg tagtgcagcc
     2941 ttagactatt cgcgaagaaa tcatcatgat gtcattatcg tagatactgc aggacgttta
     3001 ggcatcgatg agttcatgat gaatgaaatt agtgagctcg aggcgttact caaacccatt
     3061 gaaactttct ttgtggttga tgcgatgcaa ggtcaggacg ctgttaatac cgccaaagct
     3121 tttgctgatg cactaccttt gacgggcgtc atcttaacta agctagatgg ggatatgaga
     3181 ggtggagccg cactatcagt tcgacatgta accggtaagc ccattaagtt tgtagggatt
     3241 gctgagaaac taacgggctt ggaaccattt tttccagatc gaatggcttc tcgtatttta
     3301 ggaatgggtg atgtactggg tttgattgaa gatgcccagc gtagttcaga ccagaaagaa
     3361 gctgaacaat tgatgaagaa aatgaaatca ggcaagggct ttgacttgaa tgattttaaa
     3421 atgcaatttc agcaaatgaa aaagatgggt ggaatgactg caatgcttga aaaattaccg
     3481 catcaattga gtcaggctgc acagaacgtt aaagtggatg atcgcattat caaccgcaac
     3541 gaaggaatca ttaattccat gacgcaactg gagcgtacta aaccagaatt aattaaagca
     3601 tcgcgtaaac ggcgtatcgc agctggttca ggggtttcag tgcaggaagt caaccggtta
     3661 ctagctcagt ttgagcaagc tcaaaaaatg atgaaaatgg ttaataaggg aggtatggct
     3721 aagatgatgc gtgccatgaa aggcattctt ccgcaattcc gttaattttg ttggaggtaa
     3781 ggaagcaccg tatcattcga ttgcatcggg tacattttca agtccgatta ttttatgccc
     3841 ggcagtaatt tttctgcttg taaaccaagc ttgatgcatt tcaagcgcgt attttgccgg
     3901 tttgatcgaa gcgtgtgcta ctgttgaaaa gggctccatt gcttcaatat tgagaatgat
     3961 gcctttttca tcaataaaag caacactcaa gggtagcgac gtattgagca tccacatgct
     4021 gtagaagtca attttaggaa aaataaagag cataccctgg ttttcaggca gcgtggcgcg
     4081 gtccatgagc cctttggaac gggtatcata ggtatgagcg atttccgcat tgaggggatg
     4141 accgccaatc gttaacgaaa ctaccagatt ctcagccagt aatagcggac tcagtgtcaa
     4201 acccaagcaa aagctgagta taaatagatt agatctaatc atcggccagt gctaccgaaa
     4261 ccaccttcac cgcgatgact cattgcaaag ttttctacga tattaaactg cgcttgtacg
     4321 acaggaataa caaccagttg ggcaattcgt tctaaaggat tgagcaagaa ttcagtttgc
     4381 ccacgattcc agcatgatac aaaaatctgc ccttgataat cagaatcgat caacccaacc
     4441 aaattaccta agacgatacc gtgcttatgt cctaaaccag aacgaggtag aacaaccgca
     4501 gcaaacccag ggtcttcgat gtggatggca atgccggtag gaatcaattg tgtttcgccg
     4561 gggtggactg ttaatggttg ctcggtgcag gcgcgcaggt ccaatcctgc cgaaccgggg
     4621 gtggcatagc taggaagttg agtttgtagt cgtgtgtcca gaattttgat atcgattttc
     4681 atgagtcaaa ctcagcagta tatgctgaag tagcaagaaa ttccccacaa ctaatttgtg
     4741 gggagtaagg taatgtttca gtaaggtggt acttaaatta cttgctttta ctggttaatg
     4801 aacctaagaa tgcttttaca tcttcaataa acccagggcc tacttcgcgg cctagttggt
     4861 gctctgccat ttcttcaatc gcttgttcta gcgtggcaat agaaccatca tggaaataag
     4921 ggccagtttg ggcaatattt ctaaggcttg gcactttaaa tactttctta tcagtttcta
     4981 aaccggttac ctcatatctg ccgagatctt tggtttcata cggttcaacc aggccaattt
     5041 ttttgtatga attaccaccg atggctacgc cattatggca atttgcacat cccatgtgga
     5101 cgaattttgt caatccgcgt ttttccgcat cattcaatgc attctcatca cctttgagaa
     5161 aatcgtcaaa acgcgaaggg gttaataaag tccgctcgaa tgcaccaatc gctttgccaa
     5221 tatttttata ttgaataggg tctttttcgt ccttgaatgc attcgcgaac agagttttgt
     5281 actcatcgat ttttttcagc ttgttgacca ccgcttcttc attgggcatg cccatttcga
     5341 ttggattcag aatgggacca agtgcttgtt cttcaacatc tttggcacgc ccatcccaga
     5401 attgagcaat atgaagtgca gcgttaaatg ttgtggggga attccgacca ccgcgtgtgc
     5461 catcatgacc gggtgaagta ggttggctat caacaccaaa attgtttaat tgatggcaag
     5521 aattgcaaga aatcgtatca ttgatcgaaa gccttggatc aagatatagc tttttaccaa
     5581 gcgcaatcag cgtggcattt ttcttttcat cgatgatgga tgcgggaagt ggttcgaaga
     5641 aatgctttaa tgtagcacca tctaatttgt ggtgtccacc atgcatcatc ataccgccca
     5701 taccatgatg gccatcacca tgaccttgca taccaccctg ctgggtgtct tctttttgtg
     5761 atttatgttg atgatcgtgc ccactttgtg cataagcata agaacttact atgagcacta
     5821 agcataatat tactctcata cagcctccaa gaaattaacg ttagtaatga ctggtcacat
     5881 actcaaatta ttttatggat tctaaaagtt agaatgagta gtttacaact gaagatcata
     5941 tcaaattaat cttgtaatga cttaacatga tttcgataat ggagaattaa ttttctcatt
     6001 gcttaaatca ctttgtcttg agaatttctg aggtgattta agttgttatg tatgtgtttg
     6061 gctatttatt gataaatttc tgtggcgagg cgtaataatt gtgagcttaa atttaagcca
     6121 gctttttttg ccatcattaa attagcttca aaggtgactt tctcattatt gacattcata
     6181 tttagtatca cgccctgatg acaggcgcca ggactgtcag ctatggttaa aattggcttg
     6241 tctttgactg tagtaatcac atcggctaag ttagaaatca taggacgcga aataaaaaca
     6301 agctgacact gatcaagttg gctcacatta tcgattctat gaatgtcaat cgtgcgctca
     6361 ttaatttttt tacttttcat taaaaaatta atatcttgac caaaaggatt atcgccataa
     6421 atgcacagat taaatttatc gatattgcta ccaggccagg aggtaaatat tgcgaagtta
     6481 tacaaaaaga gggcttttaa gccatattcc gatacttcct cagcttgcaa aggcttgggt
     6541 agaatagcgc ttatgccaat gagcaataag taaaggattg cacttggttt tttatgtgaa
     6601 aacaagctgc ctaagttgta aaaatattgc attagtaact tgagcatcgc tttcacaatg
     6661 gatttgatca gaatcgccaa gtaactttac caaatacacc gcgttgaatc tgcaccggca
     6721 tggtttgaat gacttcgggg gaaaattcct gatggtgtga ttgcaataaa ttctgtccaa
     6781 ccagggttaa ttccgtatct gatcttggcc gccacgataa tcgtaaatct agcgcttggt
     6841 aactctttat ttccaaacca ggtaatttat cgatgtgtcg gaagaacgca tccagctcaa
     6901 tattcttggt taaatcaagt tgtgtgcgga gtgtaaattg atgcttggga tcttcgccgg
     6961 attccgactc atgatccgca ggcacatgat ttcttacttg taagtgacta tagcttggca
     7021 tgaagcgtag cttatcaaag gcacgccatt ctccataaat ctcaaatcca aagatttgag
     7081 tcttgcgttg attggtaagt tgaattgttt ctgcggttgt ttctcgttcg ctggtagaat
     7141 tattatagtc attgaaaaaa cctgcgatgt cgactgataa atttgaagta acttgacgtc
     7201 gatagcccgt ttcaaatccc catagtgttt ctgcaagcag atgaggatta ctgatcaaac
     7261 gaataaattc ggcgccactt ctgatgagtt ggttgccatc gcgttcgaag cgcgagggta
     7321 aacggatcga acgtgttgcg gataaccaca cagattgctt gtcatctggc gtccataata
     7381 gtctagcatt aggtaagaaa ttcgaatgtg cgtaagtgtt atcaagaaat ttaattcctg
     7441 tggtgagttt tagccgatct tcaaatagcg gtatgtcggt ttgcatgaag acattgccag
     7501 tctgtaagtt acgttttgct ggatcgaagg aaatcggtaa gttaattccc aaatgatcgg
     7561 tagtccagcg atagcctcct ccccaaatca gatccggctg gaatggaata taaggaattt
     7621 gtaatggaaa acggtgctgg aaatcgatgt caacggtttg tctggtttcc cgaaaagaca
     7681 attcattgcg agatgtgcga tcataataaa cttgtagtgc cgtatttgaa cgctctccag
     7741 tcgttcgctg ccagcgcatt aatgcatgcc caccagcgag attggcatct tcagggcgcg
     7801 taatggctga ctcaggttca gtagggttga gaagcatggt attctggcca gcctttccag
     7861 catagtattg cgcttgaaag gtaacggcat tattggtatt gatatcccaa tcggcccgaa
     7921 aaccgagggt tcccatgcgc caactgtcgt ttgtaccgct tgcattgaag ctgttatcac
     7981 gggtaaatcc cttagcataa aaacggtaaa aaagattttc ccccacacgt ccaccatggc
     8041 ggaaagtagc gaaccctcgc tcttcactac cacccccagt ggtgattaaa ttgccttgag
     8101 tcgctttagt atgtttggtg atgatattaa tggcaccgtt gacagcgtta gcgccccaaa
     8161 ttgttccacc aggcccccga atgacttcga tgcgatcaat atcctccaag ggtagatcga
     8221 gcgtttccca aaatgtaccc gcaaacagcg gtgaataaac agtacgtcca tcgatcataa
     8281 cgaggagatc atcagcaaag cggccactat aaccacgaga agtaactgac catttgttgg
     8341 aatcaatccg tgctactgat aaacccggtg ccatgcgcaa taattcagga atgctattgg
     8401 cgctggagcg gcggatatct tcttgagaaa tcacaaaaac cgcagcggct gtttcttgca
     8461 atgtttgcgg tttccggaaa acagtagaaa ttttgacatt catcaattcc tcaagcgagg
     8521 catcagcaag attgcgtata gtctggatat tctgtgcagc aaatgcagaa ttgataaaag
     8581 gcgctaacaa cagaaaaagt gataactgcc aatgtgtttt actggcacag atgggttttc
     8641 gatacgaagt aactaccatg gtcacaaaaa ttcctttact gcttggtata gaaaatcatt
     8701 gagcatacaa gcaatgattt tcccatagcc agtctcaatt caaagactta aattagcgat
     8761 aatttgaggt ttttttgatt ggaagaatgg ggttgatgct gatatgttta actccagatt
     8821 tccatgagtg gcgtgcaaaa tcatgtagag actcaggcat tttgtttttc aaataactgt
     8881 gcaatatgtt taatgagctg gcgggcgagt tcggtttttg aggcctgggg taaaacatgt
     8941 ttaccgttat catcgaacaa gattaacgta ctttcgtcag cccccatcgc ttgttgggca
     9001 agatttgcta cgatcaaggg taatcgcttt tgttgtcgtt tcttttgagc atattgatcg
     9061 agtttttccg tttctgctgc aaaaccaacg caaaacggtg gtttttttaa atgagccaca
     9121 tagtgaagga tatcgggatt aggaactaac tcaagcgtta atggctggtt ggctttcttt
     9181 attttttgtg gctggtaggt ttgagggcgg taatctgcaa cggctgcgac actgataaag
     9241 atatcgcagt tgcttacttc ttttttaaca gccgctaaca tttgtgttgc gctggtaaca
     9301 ctaatgaatt tttgtaccga tggtttgggt aaatgaaccg gccctgcgat gagggtaact
     9361 gtagcacctg cattgctggc tgcgtttgcg attgcatagc ccatcttacc cgaactcaaa
     9421 ttgatgatac cgcgcacggc atcgatggct tcatacgttg gtcctgcggt aatcagaaca
     9481 ttttttccat caagccattt aggctcaaaa aaaacctgta aagcctgttg taattgctcc
     9541 ggttcaagca tacgccccag tccattttca ccacaggctt gatcaccact agcaggaccc
     9601 cagatggtta tctgatccgc atgcaaggta ttgaggttac gctgtgtcgc tgggttttca
     9661 aacatctgct tattcatggc tggtgcgatg atcaaagggc aatcccgcgc taagcaaagt
     9721 gtagaaagca aatcatcggc gacaccgtgt gcgagtttcg cgatgaaatt agcactggtc
     9781 ggtgcgatga gtatcgcatc gctactgcgg gataatccga tatgagccat ggaattttgt
     9841 agggaatctc cccaaagctc ggtgtaaacc ggattccctg ttaacgcatg gagggtttct
     9901 ggccctatga atcggcaggc agcttgggtc atcaccgttt gaatgatggc cccttctttg
     9961 actaaaagcc gagcgagttc agcagttttg tacgctgcga tacctcccgt tatgccgagt
    10021 atcagttttt taccggtgag cgcgctgatc gaatccatcc tgcttgcaaa ttaacaaatt
    10081 gagattggtt atgacatagc cttaataata caacgactga tcatattacg atgatttttg
    10141 actttatgta gttttatttg gaatgaccgt ttacatggct catggttaac gcgatggaga
    10201 aatatgatgg caattagtga ctggcctgag ctagaacgtc cccgtgaaaa attattgcac
    10261 tatgggcccg ctagtctttc cgattcagaa ttactcgcta ttttcttacg caccggtatt
    10321 actggaaaga gtgcggttga tcttgcaaga gaactgctga gtcattttgg gagtttgacc
    10381 gagttattca gcgcagatca gcgatcattt tgtcaaatac caggattggg tgtagccaaa
    10441 tacgtacaac tcaaagcaac gctggaaatg gcgcagcgtg cgctcgaaac gggtttaaag
    10501 cggggcgata tcatgacttc tccgcagcaa gtgcggcaat ttttgcgact tagcttggtc
    10561 catcgtcaac atgaagtgtt tatgggactc tttttagatg ccagaaatcg cattatccaa
    10621 agtgaagaat tatttagtgg aacactgaca gaaacgagtg tttatgcgcg agaagtcatt
    10681 aaacgtgcgt tatttcacaa tgctgcagcc atcatttttg cgcataatca tccttcagga
    10741 catgccgaac cgagtcaagc agatgaagta ctgacccaat ctttgaaaca agcactggcc
    10801 tatgtggaag tcaaagtgct ggatcacttt attgtcggtt cggaaggcat tacttcattt
    10861 gcggagcggg gcttactttg aagttaatgg gtagtgagcg atgtgtgcgg taagcgaaaa
    10921 agggtattaa agttatccgt tgttgcggtc gcgatttcat cgaggctttt ttcgcggagt
    10981 ttggcaattt cttcagccac atgtttaaca aatgcaggtt gattggtttt tccacggtgt
    11041 ggaacgggtg caagataggg cgaatctgtt tcgatcaaca tgcggtttaa gggcactttc
    11101 cgagcaactt ctttcaacat tagggcattt ttaaacgtga cgatgcctga aaaagaaata
    11161 taaaaattca tctcgatagc gcgttgtgca acatcccaat tttccgtaaa gcaatgcatg
    11221 acaccgccaa tcgttgctgc gtcttcctct tgcatgatgc gcaaggtatc ttctgctgct
    11281 tcccgggtat gaatgatgag tggtttctgg ctttccctgg ctgctcgaat atggcgacga
    11341 aagcggtcgc gttgccaatc gagatcacct ttaatgcgaa aataatccaa ccctgtttca
    11401 cccacggcaa tcactttggg atgttgagcg agttcaacga gctgagccac agttggttca
    11461 gtgatgtttt cataatcagg gtgta
//
LOCUS       DAOQKQ010000078        11482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_2641_length_11482_cov_6.229982, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000078 DAOQKQ010000000
VERSION     DAOQKQ010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 11482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11482
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2641_length_11482_cov_6.229982"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1235)
                     /locus_tag="PKZ49_06600"
     CDS             complement(<1..1235)
                     /locus_tag="PKZ49_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HND36268.1"
                     /translation="MTYSQSTSTQFSSTVMAPELKFGVSFLELYRRDGLLKLDQAFLE
                     FLGSADPVLRARLDQARLSPNEIAPKDESILLIDLAPWAEDFIGQLFGIEKEVRALAA
                     KHHELAPLYFCKRQFVQRRAKTKVSDEILSTIDGIALEKALEVEFGEPFSELVFATHV
                     ANWLDDEANNAARIEKALHYAAWALRTPEGQMHTQAGILFKTPAKLDFLHLLSLSTDN
                     EKGFPLHRLDHLRHREGFSLTDTGTDLVGALDEANYCIWCHEQGKDSCSKGFIQKPKS
                     SEELPSFKKSELGVLLAGCPLEERISEFHKLKTQGHAIGSLAMIVLDNPMCAGTGHRI
                     CNDCMKSCIYQKQVPVNIPQAETRTLKDVLELPWGFEIYSLLTRWNPLDLRRPLPKPA
                     TGKKVLVVGMGPAGYTLAH"
     gene            complement(1380..2651)
                     /gene="cysN"
                     /locus_tag="PKZ49_06605"
     CDS             complement(1380..2651)
                     /gene="cysN"
                     /locus_tag="PKZ49_06605"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HND36269.1"
                     /translation="MSVVENVGLDRAELLRFIAAGSVDDGKSTLIGRLLHDSKSIFED
                     QLSSIAKTSSKRGMQNVDLSLLTDGLQAEREQGITIDVAYRYFATPKRKFIIADTPGH
                     EQYTRNMVTGASTANLAIILIDARKGVLTQSRRHTYLASLVGIPHIVVAVNKMDLVEY
                     SQEVFTQICDDFAVFSQHLNLRNIEYIPMSALNGDMVVERGDQLDWYQGRTLIDLLEN
                     VDIEHDVNLDDFRFPVQWVCRPQTEELHDFRGYMGRVESGSIKVGDAVTIQPSGLHSR
                     IKEILLYQSNFDEAFAPQSVTVTIEDHLDISRGDMLIKSDDQPRVDKEFTAMLCWLSE
                     QPLSSGRKYIVKHTTRMVKGLVSQIDYRVDVNTLNQEKTDSLRMNDIARVVMKVQQPL
                     VFDAYSRNRATGSFILIDETTNNTVAAGMIC"
     gene            complement(2712..3617)
                     /gene="cysD"
                     /locus_tag="PKZ49_06610"
     CDS             complement(2712..3617)
                     /gene="cysD"
                     /locus_tag="PKZ49_06610"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HND36270.1"
                     /translation="MNHRSFTHLDALESEAIHIMREVAAECANPALLFSGGKDSIVLL
                     RLAEKAFRPGRFPFPLLHIDTEHNFPEVIAFRDHRAKELGERLIVRSMDDSIKKGRVV
                     LRSENQSRNPFQSITLLDAIAEFSFDACIGGARRDEEKARAKERIFSFRDEFGQWDPK
                     NQRPELWSLYNTKTHSGENIRVFPISNWTELDVWEYIARENLEVPSIYFAHWREVIPR
                     DSGLLPVSHLVQPRDNEKIEKIMVRFRTVGDMSCTCPVASDAASVGEIIAETAMTRIT
                     ERGATRMDDQTSDASMELRKKEGYF"
     gene            complement(3652..4353)
                     /locus_tag="PKZ49_06615"
     CDS             complement(3652..4353)
                     /locus_tag="PKZ49_06615"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HND36271.1"
                     /translation="MTEINIEHKVEEVSRLLEDIHENFMPAVFANSFGAEDMLLTDLI
                     ARYFPNIEIFTLDTGRLPEETYALIQTVRDQYHINIKAYFPEAAAVESYIAQNGPNGF
                     YQSVELRKRCCYIRKVEPLRRALTGKRAWVTGMRREQAITRDSLSISQFDAEHAIQKF
                     NPLCDWSNAEVWSYLKQHDVPYNKLHDHFYPSIGCAPCTRAITPGEDIRSGRWWWENP
                     DSKECGLHVSRAVSA"
     gene            complement(4382..6478)
                     /locus_tag="PKZ49_06620"
     CDS             complement(4382..6478)
                     /locus_tag="PKZ49_06620"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HND36272.1"
                     /translation="MDYLTDLANDEEIDSYNQSLQDFFAQKMDVDRFTATRLQMGIYG
                     QRQDGVNMVRIKVPGGRLNISQLDAIADVIEKHVQSGEAHVTTRQDIQLHYVPLADTP
                     AVIRHLAKGGLTTREACGNTIRNVTACPLTGVCSREHTDISQHLGGVVRHFLRKPLNQ
                     LMPRKFKMSFSGCEADCAQAMIHDLGVVAVQQGDRFGFKVLVGGGLGHKPHEAIVIES
                     FAEEKDLLLIVEAVLTMHNKYSDRNKRAKARIKFLVDKFGVDGFVEKYREELARVKAV
                     LADQDYPKGDWALGNRASEVPGPGAPRKLFAQKQAGLFVVPISVPLGQVTAKQVRGIA
                     KLLRQHDLKEIRTTQDQNLMLVDVPEAKITDIQATLNELGLSLPKAGDDVVACPGTST
                     CRLGITSSPTVGAKLTGGKDDLRIRVSGCHNGCAQPESGDIGIYGEGKRMYGKLVPHY
                     QMYFGGNGMAEGGLAFKGPSVPSARVEVAVDRVQSAYATNRQNGESFFNWTRRVGQGY
                     FTELLADVIEVKQEDLHAVLRDHGQGDDFRVLQLGGGECAGAKQLQIGTAFFDAANER
                     NYRNALKFQRKLEDSLKCAEEIARLISQGLAQLVGGKKFENLSEQAEELKRVLPTKPN
                     LAVGLAKFAQAFSTPAEELTDSALTEWYAALDEWTAEVADFCIDFDRQLDLAGALPKA
                     VSSIPMKHAQQSTTAL"
     gene            6779..7729
                     /gene="cysB"
                     /locus_tag="PKZ49_06625"
     CDS             6779..7729
                     /gene="cysB"
                     /locus_tag="PKZ49_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HND36273.1"
                     /translation="MRLQQLRLLCEINRQNLNLSKAAESLHTSQPGISKQIQLLEEEL
                     GVDIFVRNGKRIVKITPPGKAIIEIAERMLQDAENLKSVGQEFNNQTSGTLTIATTHT
                     QARYALPPVIKRFTERYPNVKLTLRQGNPTQIATLVSSGEADLAIATEAIELFHDLIM
                     LPCYQWNRCIVVPPNHPLLDSPILTLEAIAQYPIVTYDFAFTGRSKINRAFEANGLNP
                     NVVLTAIDSDVIKTYVELGLGIGILANMAFDPLRDQHLRSIDASHLFEPSTTRVGISR
                     NSYIRGYSYDFIEMFAPHLTHDVVKSAMKGKPNKTEPFSF"
     gene            8101..9183
                     /locus_tag="PKZ49_06630"
     CDS             8101..9183
                     /locus_tag="PKZ49_06630"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36274.1"
                     /translation="MEKLNIAYTCNVRKPGMEDERYGEFESPETIEAVISALEGAGAN
                     VELIDVGPDIFQQLEKRKNSIDIVFNNTEGLNEKELREAIVPFFCEFLSIPYTGSSPK
                     TFINKMDKATAKRIVAYDGVPTARFQLMKPQDRLRDLAFPLMVKPYSEGTSIGINQKS
                     KVNNLAELEQAIETIFTQFNQPALVEEFLDGTEYTIGFIGNYLLPILEIDFAKIPGQP
                     QLRDPYVKSIENPFISHLPWNEKARSFASLSIKAHEALEVRDYNRMDFRERNGQLYFL
                     EANVIPGLHPTEADLTNMCRHAGVAHADMVVLILSAAVKRLSEQYPERFKKKTELLTE
                     LANKAICKATHAGEIVYQDRVYNLLG"
     gene            9204..9659
                     /locus_tag="PKZ49_06635"
     CDS             9204..9659
                     /locus_tag="PKZ49_06635"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HND36275.1"
                     /translation="MIVPISQEMHPQLIKIALQEMGAMYQPELHEELDDIERGRCFGL
                     CKLENGKPVGYVVAKRTIDAYHLLTIAVDIEHRGKGYGSLLIDSIFAEIEKQGGRILN
                     VITDAEANESIRFYLKNSFVLAGIVQDEFISGVAQVHLTKRAGYSPEQY"
     gene            9715..10551
                     /gene="dapF"
                     /locus_tag="PKZ49_06640"
     CDS             9715..10551
                     /gene="dapF"
                     /locus_tag="PKZ49_06640"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HND36276.1"
                     /translation="MRIRATKMDGCGNSFLVIDEIKNPLARFDRTKISNALAVQHNTD
                     GILYLDQQHGMPTMKIFDRDGTEETMCGNGLRCATRYFRDQYISSNIFPIVTGDGIKE
                     VTVDNELVEVNLGSARDYCQVSDKMHFVFTGVPHLVVIGNEMSLESAKTLGKLLREDK
                     SLCARVNHPEGINVNFIWRTIEGIAVRTYEVGVEDITLSCGTGSAASAYVAAKVYDLS
                     LPITTQSLGGNLEISDIGGELSIRGITRYQGKVIGFVNHAHPKSTIPSRYWQRSNVSL
                     SL"
     gene            10577..10873
                     /locus_tag="PKZ49_06645"
     CDS             10577..10873
                     /locus_tag="PKZ49_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36277.1"
                     /translation="MEFSIVTFLLIMVAMAFADVCWTLYFIDVEERKAHPAAFWSAMI
                     ILVTAFSVTNYVENKIYIAAAFIGAYIGTYGTLKWKLHKEKHHAQSESSQTKGA"
     gene            complement(10901..>11482)
                     /locus_tag="PKZ49_06650"
     CDS             complement(10901..>11482)
                     /locus_tag="PKZ49_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HND36278.1"
                     /translation="PSLYSRKEASVTDWSLEAIIELLSSGVSKHANVSGPMATVVQQS
                     LQYWSPQDIRAMAVYLKSLPDKTAQENVRYPEMPESAKRYLAQGEKVYEKHCQDCHGE
                     VGQGIPGIYPALANNRSVTLPVPLNLIRAVLNGGYPPTTHLNPQPYGMPPFQQLLRDD
                     EIAQVISYIRNAWGNRAGLVTIVDVDQGRGGNH"
BASE COUNT         3421 a         2723 c         2149 g         3189 t
ORIGIN      
        1 tggtgtgcaa gcgtgtagcc agccggtccc ataccaacaa ctagtacttt tttacccgtt
       61 gctggtttag gtaaaggtct tcgcaagtcg agtggattcc agcgcgttaa caaactataa
      121 atctcgaatc cccatggtaa ctccagcaca tctttgagtg ttcgggtttc tgcttgagga
      181 atattaaccg ggacttgctt ttgataaata catgatttca tgcaatcgtt acagatacga
      241 tgcccagttc cggcacacat cggattatcg agcacaatca tcgctagact accaattgcg
      301 tggccttgtg ttttaagctt atgaaactct gaaattcttt cttccaacgg gcaaccagct
      361 aataaaacgc ccaactcact ttttttgaat gaaggtaatt cttccgagct tttaggcttc
      421 tggataaatc ctttagagca agaatcctta ccttgttcat gacaccagat gcaataattc
      481 gcttcatcca gtgcgcctac caaatcagtc cctgtatctg tcagtgaaaa cccttctcgg
      541 tgacgtaaat ggtctaaacg atgcagcgga aagccctttt cattgtccgt cgagagcgat
      601 aataaatgta aaaaatctaa ttttgcgggt gttttaaata aaatacccgc ttgagtatgc
      661 atttgaccct caggagttct taaagcccaa gctgcataat ggagggcctt ttctattctt
      721 gccgcattgt tcgcttcatc atccaaccag ttagctacat gagtagcaaa taccagctct
      781 gagaaaggtt cgccaaattc aacttctaag gctttctcaa gcgctattcc atcaatcgtc
      841 gataatatct catcacttac tttagtttta gcgcgccgct gcacaaactg tctcttacaa
      901 aaatataaag gcgctagctc atgatgcttt gctgctaaag cacgaacttc tttttcgatt
      961 ccaaataatt gaccgataaa atcctctgcc cagggagcga gatcaattaa caatatcgat
     1021 tcatcctttg gcgcaatttc atttggactc aaacgtgctt gatctaaacg agccctaagt
     1081 accggatcag cacttccaag aaattctaaa aatgcctgat ctaatttcag cagaccatcg
     1141 cgacgataaa gctctaagaa actgacccca aatttaagct cgggagccat aactgtcgat
     1201 gaaaattgtg tagatgttga ttgtgaataa gtcattttgg ttaattttta ttgtaaaaat
     1261 cctactaaaa ttttgctttg gttaaactca atacaaatct ttcagaagat ctatgcaaat
     1321 tgccagcgct ctagcaagct tgaatcaaag caagggagat tacaaaatag gattggactt
     1381 tagcagatca ttccagctgc aaccgtatta ttagttgttt catcaattaa aataaaacta
     1441 cctgttgcac gattgcgact gtatgcatca aaaacaagag gttgttgaac tttcatcaca
     1501 acacgcgcaa tatcgttcat ccttaaggag tctgttttct cttgattaag cgtattcaca
     1561 tcaacacgat aatcaatttg actcacgagc ccctttacca tacgcgtagt atgcttaact
     1621 atatatttac gtccggaact caaaggttgt tccgacaacc aacacaacat cgctgtaaat
     1681 tctttatcga ctcgtggttg atcatctgat tttattagca tatccccacg agagatatcc
     1741 aaatgatcct caattgtcac tgtaacagac tggggcgcaa aggcttcgtc aaaattgctt
     1801 tgataaagca aaatttcttt aattcgagaa tgtaatccag agggctgaat cgttaccgca
     1861 tcaccaacct taattgaacc ggattcaact cgacccatgt aacccctgaa atcatgaagc
     1921 tcttcggtct gaggccgaca aacccattga accgggaaac gaaagtcatc caaattaaca
     1981 tcgtgctcaa tatcaacatt ctccaacaaa tcgattagcg ttcttccttg ataccagtct
     2041 agttgatcac cgcgttctac aaccatatcg ccatttaagg ctgacatcgg aatatattca
     2101 atgttacgca aattaagatg ctgcgaaaac actgcaaaat catcgcaaat ttgagtaaat
     2161 acttcttgtg aatattccac taaatccatc ttatttactg ccacaacaat atgaggaata
     2221 ccgacaaggc tcgcaaggta agtatgacgt cgagattgag tcaagacccc cttacgtgcg
     2281 tcgataagaa tgatcgccaa atttgccgtg gaggcacctg ttaccatatt tcgggtatat
     2341 tgctcgtgac cgggtgtatc cgcgataata aatttacgtt taggtgttgc gaaataacga
     2401 tacgccacat cgatcgtaat gccctgctcg cgttctgctt gcaaaccatc cgtcaataac
     2461 gacaaatcaa cattctgcat accacgtttg ctggatgttt ttgcaataga gcttagctga
     2521 tcttcaaata ttgattttga atcatgtaat aatcgaccaa ttagcgtact tttcccatca
     2581 tctacactac ctgccgcaat aaagcggagt agttcagcac gatcaagccc tacattttca
     2641 actaccgaca tttgataacc ttctatatta aacttttaga ttgatttatt ttctgtatca
     2701 gattcacaaa actaaaaata gccttctttt tttcgcaatt ccatcgaagc atctgaagtt
     2761 tgatcatcca tgcgagtcgc acctctttca gtaatgcgag tcattgccgt ttcagcaata
     2821 atttcaccga cactagctgc atccgatgca accgggcaag tacagctcat atcccctact
     2881 gtgcgaaacc gaaccatgat tttctcaatt ttttcattat ctctcggttg tacaagatgc
     2941 gaaacaggca ataggcctga atcacgagga ataacctcac gccaatgtgc aaaataaata
     3001 gatggaactt cgagattctc gcgagctata tattcccaca catcgagctc agtccagtta
     3061 ctaattggaa acactcggat attttcacca gaatgtgttt tggtattgta caaactccat
     3121 aattcaggac gctgattctt cggatcccat tgaccaaact catcccggaa agaaaagatg
     3181 cgctcttttg cgcgtgcttt ttcttcatca cgtctagctc caccaatgca agcatcaaag
     3241 ctaaactcag caatcgcatc tagcaatgta attgactgaa atggattcct actctgattt
     3301 tctgaccgca gtaccactcg acctttttta atcgagtcat ccatacttct aacgatcaat
     3361 ctctctccta gctctttagc ccgatgatcc cgaaatgcaa ttacttcagg gaaattgtgc
     3421 tctgtatcga tgtgcaagag cggaaacggg aatcgacctg gacgaaacgc tttctctgcg
     3481 agacgaagca atacaataga atcttttcct cctgaaaaaa gtaaagcagg attggcgcac
     3541 tcagcagcca cctcccgcat gatgtgtata gcttcacttt ccagggcatc cagatgagta
     3601 aaagatcgat ggttcatttt tttactaaat caatataaat taattatcta ctcaagcact
     3661 tactgctcga cttacgtgta atccacattc ttttgagtca ggattctccc accaccagcg
     3721 cccagatcga atatcttccc ccggcgtgat agcccgagta caaggcgcac aaccaatact
     3781 aggatagaaa tgatcatgca gcttgttata gggcacatca tgttgcttga gatagctcca
     3841 aacttcagca ttactccagt cacataatgg attaaacttc tgaatcgcat gttcagcatc
     3901 gaactgagat attgataaac tatctcgtgt aatagcttgc tcacgccgca tacccgtcac
     3961 ccaagcgcgt ttgcctgtta gagctcgtcg caagggctca accttgcgga tatagcaaca
     4021 acgtttacgt aattcaacac tttgataaaa cccattggga ccattctgcg caatataact
     4081 ttccaccgca gccgcttcgg gaaaatacgc cttaatattg atatgatatt gatccctaac
     4141 cgtctgaatc agtgcataag tttcttcagg gagtcgaccc gtatccagcg taaatatctc
     4201 aatattggga aaatagcgtg caatgagatc tgttaaaagc atatcctcag caccgaagct
     4261 atttgcaaat acagccggca taaagttttc atgtatatcc tcgagtagcc gggatacttc
     4321 ttcaacttta tgctcaatat taatttcagt cattccatta attagcgttt atctacgaaa
     4381 cttatagtgc agtagttgac tgctgagcat gcttcattgg tatcgatgac acagctttag
     4441 gcaacgcccc tgccagatca agctggcgat caaaatcgat acaaaaatca gccacttcag
     4501 cagtccactc gtcaagcgca gcataccatt cggtcaaggc gctatcggtt agctcttctg
     4561 ctggagttga gaaagcttgt gcgaatttag ctagcccaac tgcaagatta ggtttcgtcg
     4621 gcaaaacacg cttgagctcc tcggcttgct ctgacaaatt ttcaaatttc ttaccaccca
     4681 cgagctgcgc caaaccttga ctgatcagtc ttgcgatctc ttcagcacat tttaaggaat
     4741 cctctaattt acgctgaaac ttaagtgcat tacgataatt tcgttcattc gccgcatcaa
     4801 aaaaagcagt accaatttgg agttgtttgg cgccggcaca ctcaccccca cccaattgca
     4861 acaccctaaa atcatcccct tgaccatggt ctcttaaaac agcgtgtaaa tcttcttgct
     4921 taacttcaat cacgtccgcc agcagctcag taaaataacc ttgcccaact cgcctggtcc
     4981 aattaaaaaa cgactcacca ttttggcgat ttgttgcata agctgattga acgcgatcaa
     5041 ccgcaacttc aacacgcgca cttggtaccg aaggcccttt aaatgccaac cccccttcag
     5101 ccataccatt gccaccaaaa tacatctgat aatggggaac aagtttgcca tacatccgct
     5161 ttccttcccc ataaatccca atatcaccac tttcaggttg tgcgcaacca ttatggcaac
     5221 cagaaactct aatacgcaga tcatctttac cgcctgtcaa ctttgctcca acagtaggac
     5281 tagaagtgat gcccaaccga cacgtcgacg ttcctgggca agcaacaaca tcatcccccg
     5341 ccttgggcaa actcaaccct aactcgttta acgtggcttg gatatctgta attttagctt
     5401 ccggcacatc aaccaacatt aaattctgat cctgagttgt tcggatttct ttcaaatcat
     5461 gttgcctaag caactttgcg atcccacgca cctgcttggc cgtaacctga cccagcggga
     5521 cactaatagg taccacaaat aaccccgctt gtttctgggc aaacaattta cgtggtgccc
     5581 ctgggcctgg cacttccgat gctcgattac ctaaagccca atcaccttta gggtagtcct
     5641 gatcagccaa cacagcttta acccgcgcta attcctcacg gtatttctcg acaaacccat
     5701 cgactccaaa tttatccact aagaatttaa tccgagcctt agctctttta ttgcggtcag
     5761 aatacttgtt gtgcatggtc aacacagctt cgacaatgag cagcagatct ttttcttcag
     5821 caaacgattc aattacgata gcctcgtgtg gcttatgtcc caaccccccg ccgactaaaa
     5881 ccttgaaacc aaaccgatcg ccttgttgta ctgcaaccac tcctaaatca tgaatcatcg
     5941 cctgcgcaca gtcagcctca caacctgaaa agctcatctt aaatttgcgc ggcattaatt
     6001 ggttaagcgg tttacgtaaa aaatgtcgga ccacgccgcc caaatgctga ctgatatcgg
     6061 tatgttctcg tgaacagact ccagttagcg gacatgcagt tacattccga atagtattac
     6121 cgcacgcttc tctcgttgta agcccacctt tggctagatg ccttataact gcaggtgtat
     6181 cggctagcgg cacataatgt agttgaatat cttgccgcgt cgtaacatgc gcttcaccag
     6241 attgaacatg cttctcgata acatcagcga tggcatccaa ttgtgatata ttcaatcgtc
     6301 cacccggtac tttaatacgc accatattaa ccccatcttg gcgctgtcca tatattccca
     6361 tctgtaaccg ggtagcagta aatcgatcaa catccatttt ctgcgcaaag aaatcctgca
     6421 atgattgatt gtaggagtca atttcctcat cattagccag atcagttaaa taatccatct
     6481 atttcttctc tccatttttc actcatatca acttgctact gatatgtaag taaggtatta
     6541 ataatttacg cctattttat ctgatatctg atcgctggcg gtaatttctc gatcacttcc
     6601 cttctatttt ttatgagtcc caattggagt ctgtcgcata ttgggctatt gatcgatcaa
     6661 atagatacga ctgcagttat attcagggta agaaagtaag ctatggtatc agcacttaca
     6721 tatacctcaa aataatatta tattacaata atataattat taattatata agaacacgat
     6781 gagactgcaa caattacgtc ttttatgtga aatcaatcgc caaaatttaa atctttccaa
     6841 agcagcagaa agcttacaca cctctcagcc cggtattagt aagcaaattc agttactcga
     6901 agaagagctc ggcgtagata tttttgtacg caatggtaag cgtatcgtta aaatcacccc
     6961 ccctgggaag gcgattatcg aaattgccga acgtatgcta caagatgctg aaaacttgaa
     7021 atcggttggc caagagttta acaatcaaac cagcggcaca ctcactatcg caaccactca
     7081 tactcaagcc agatatgcgc taccaccggt cattaaacgc tttacggagc gctaccccaa
     7141 tgtaaaactt accctgcgac aaggaaaccc tacccaaatc gcaacactag tctcatctgg
     7201 tgaagccgat ctagccatcg ccaccgaagc aatcgagcta tttcatgatt taatcatgct
     7261 accgtgttat caatggaatc gctgtattgt cgtaccaccc aaccatccac tcttagatag
     7321 cccaatcctc acacttgaag ccatcgcaca atatcctatt gttacctatg atttcgcatt
     7381 cacaggccgt tcaaaaatta atcgggcctt cgaagcaaac ggattaaatc caaatgtagt
     7441 tttgactgct atcgattccg acgtaatcaa aacttatgtt gaattaggat taggcattgg
     7501 gattttagcg aacatggcct ttgatccttt acgcgaccaa caccttcgat caattgatgc
     7561 tagtcacttg ttcgaaccca gcacgactcg agtcggtatt agccgcaaca gctatattcg
     7621 agggtatagc tatgatttca tagaaatgtt tgccccgcac ctaactcatg atgtggttaa
     7681 atcggcaatg aagggcaagc ctaacaaaac tgagcctttt tcattctgag cgcagaaaaa
     7741 cttcattccc cccaatatac aatagcagac cctccatttc tgaataccta gctttgctgt
     7801 tttttcaata caaacaaaag ctatgccatt gatctcccag cttatcaaaa aaaataaccc
     7861 tattgtcaac ggaaccctat ttgatcgcgt tattggtacc tataaagcta caaaagcaaa
     7921 ataaaagtta atcacatgag ataactataa atcacgaggg aaaaatgctt ttaaccaatc
     7981 aatataccgc gaatgccagc aaagccatcg cacccattcc aataaatcta gcctgttttg
     8041 ttattcgcta acattctcta gtttcatttt tacgatttta attaagtttc cactacagtt
     8101 atggaaaagc tcaatattgc ttatacatgc aacgtcagaa aaccgggcat ggaagatgaa
     8161 cgatacggtg aattcgaaag tcccgagacg atcgaagctg ttattagtgc gcttgaagga
     8221 gcaggggcga atgtagaatt aatcgatgta gggccagaca tttttcagca gcttgagaaa
     8281 cgcaaaaaca gcattgatat cgttttcaat aatactgaag ggttgaatga aaaggagtta
     8341 cgggaagcta tcgttccttt cttttgtgaa ttcttaagca ttccttatac cgggagcagc
     8401 cctaaaacct tcatcaacaa aatggacaaa gcaacagcca aacggattgt agcttacgat
     8461 ggagtaccaa cagcgcgctt ccaactgatg aaacctcagg atcgacttag agatctagca
     8521 tttcctctaa tggtcaaacc ttatagtgaa gggaccagta tcggtattaa ccaaaaaagc
     8581 aaagtgaata atttagccga gcttgagcaa gcaatagaaa ccatttttac tcaattcaac
     8641 caaccagctc tcgtggaaga attcctagat ggaacagagt acacgattgg ttttattgga
     8701 aattatctat tacccatcct agaaatagac tttgcaaaga ttccaggaca gccacaactc
     8761 agggatcctt acgtaaaaag catcgagaat cctttcattt cgcacttacc ctggaacgaa
     8821 aaagctagaa gttttgcgtc actttcaatt aaagcacatg aggcactgga agttcgggat
     8881 tataaccgta tggattttcg tgaacggaat ggtcagcttt attttctgga agccaatgtc
     8941 attcctgggc tacaccctac cgaagctgat ctaactaaca tgtgccgtca tgccggtgtt
     9001 gctcacgcag acatggtagt actaatccta agtgctgctg taaaaagact atctgagcag
     9061 tatccggaac gatttaagaa aaaaacagaa ttactcactg agttagccaa taaagcgatc
     9121 tgcaaggcga ctcatgctgg tgaaattgtt tatcaagacc gagtatacaa ccttctggga
     9181 taattatttt aggactctac acaatgatcg tacctatctc acaagaaatg catccccagc
     9241 ttatcaaaat cgctttgcaa gaaatggggg ctatgtatca acctgagctt catgaagagc
     9301 tagatgatat cgaaaggggg agatgtttcg ggctttgtaa gctagaaaac ggtaaacccg
     9361 tcggatacgt cgtggctaaa agaacaatcg atgcttacca cctccttacc attgctgtcg
     9421 atatcgagca tcgcggtaaa gggtatggct ccttattgat cgacagtatt tttgctgaaa
     9481 tcgaaaaaca gggggggcgg atccttaatg tgattactga cgcagaagcg aacgaaagca
     9541 tacgcttcta cctcaagaat agtttcgtct tggcaggcat cgtacaagac gaatttattt
     9601 ctggcgtggc tcaagttcat cttacaaagc gtgcaggcta cagcccagaa caatattgaa
     9661 aattcttttt acttataaca atcaaataaa ataactattc taggggcaca aaacatgaga
     9721 attagagcaa caaaaatgga tggatgtggc aactctttcc tagttatcga tgaaataaaa
     9781 aatccattgg ctcggttcga tcgaaccaaa atttcaaatg cacttgcagt tcagcataac
     9841 acagatggaa tactctatct cgatcagcaa catgggatgc caaccatgaa aatttttgat
     9901 cgagatggga cagaagaaac catgtgtggt aatggactac gctgcgctac tcgatatttc
     9961 cgcgatcaat acatttcaag caatattttt ccaatcgtta ccggcgatgg tattaaagaa
    10021 gtcacggttg ataacgagct tgttgaagtc aatctaggat cagcacgtga ttattgccaa
    10081 gtaagcgata aaatgcattt tgtgtttacc ggtgttccac acctcgttgt tataggtaac
    10141 gagatgagcc ttgaatctgc caaaacacta gggaaattgc tgagggaaga taaatctctt
    10201 tgcgcacgag tcaatcatcc cgaaggtatc aacgtcaatt ttatctggag aacaattgaa
    10261 ggaatcgctg tacgcaccta tgaagtcggt gtggaagaca ttaccctcag ttgcggaacg
    10321 ggaagcgcag ccagtgcata tgtagctgca aaagtttatg atttatccct gcctattacg
    10381 acgcaatcgc ttgggggtaa cttagaaatt agcgatatcg gcggagaatt atcgattcgt
    10441 ggcataactc gctatcaagg aaaagtcatt ggattcgtaa atcacgctca ccccaaatcg
    10501 actattcctt ctcgatactg gcaacgaagc aatgtttcat taagcctata aatccaataa
    10561 ttaatttaaa accaatatgg aattttctat tgttaccttt ttactgatca tggttgcaat
    10621 ggcttttgca gacgtttgct ggacactcta ctttatcgat gtagaagaaa ggaaagcgca
    10681 cccggctgca ttctggtcag ccatgatcat tctcgttaca gccttttctg tgacaaatta
    10741 cgtcgaaaac aaaatttata ttgctgccgc ttttataggt gcctacatcg gtacttatgg
    10801 aaccctcaag tggaaattac acaaagaaaa acatcatgcg caatccgaat catcccagac
    10861 taaaggagca taagctcaga ttcttatagc cgctccaact tcaatgattg ccgcctctac
    10921 cctgatccac atccacaatc gtaactaaac ccgctcgatt cccccaggca tttctgatat
    10981 atgaaatcac ctgtgcaatt tcgtcatctc tgagcaattg ttggaaaggt ggcatgccat
    11041 aaggttgtgg attgagatga gtagtcggtg gatatccccc atttagcaca gctctgatga
    11101 gattaagcgg aacgggtaag gtgacactgc gattattagc aagcgcaggg tagatacctg
    11161 gtatgccttg accaacctcg ccgtggcaat cttggcaatg cttctcatag actttctcac
    11221 cctgcgcaag atatcgctta gcgctctcag gcatttctgg atagcgaaca ttttcctgtg
    11281 cagttttatc tggtaacgac ttaagataga ctgccatggc tcttatatct tgaggtgacc
    11341 aatattgcaa actttgctgc acaaccgttg ccataggacc agaaacattc gcatgctttg
    11401 atacgcctga agaaagcaat tcgataatag cttcaagact ccagtcggta acactcgcct
    11461 ctttacggga ataaagagaa gg
//
LOCUS       DAOQKQ010000079        11350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_2679_length_11350_cov_5.492784, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000079 DAOQKQ010000000
VERSION     DAOQKQ010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 11350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11350
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2679_length_11350_cov_5.492784"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /gene="folK"
                     /locus_tag="PKZ49_06655"
     CDS             <1..477
                     /gene="folK"
                     /locus_tag="PKZ49_06655"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HND36279.1"
                     /translation="HIAFIGLGSNLENPVLQINRALAHLARLSEICILKQSSLYSSAP
                     IDAPNQPDYINAVVKVKTSLQPQALLAQLLAIEENSGRIRTYPNAARILDLDLLLYDN
                     LQYREKNLIVPHPRMHQRAFVLRPLVEIENDSVIPGIGLASELLNQCNQQKLERLT"
     gene            480..1127
                     /locus_tag="PKZ49_06660"
     CDS             480..1127
                     /locus_tag="PKZ49_06660"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HND36280.1"
                     /translation="MLLGKYRYIVIEGPIGVGKSSLAQNMANYFNCATLLEKPTENPF
                     LENFYHDIARYALPTQLTFLLQRTDQFKQLIETNLSNKPLIGDFLLEKDQLFAKLTLS
                     SSEYAIYQKIYQHIQPQAPNPDLIIYLQASPEILIERVKRRNHAFEKAITEDYLWKLT
                     NSYTQFFHQYKKAPILIVNSENLNFANNTKDFNLLLQQISQMRGAIEYFSCSEIH"
     gene            1140..1931
                     /gene="panB"
                     /locus_tag="PKZ49_06665"
     CDS             1140..1931
                     /gene="panB"
                     /locus_tag="PKZ49_06665"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HND36281.1"
                     /translation="MRTTRDTLQRMYAEGKKIAVLTCYDATFAHLLENVGIDILLIGD
                     SLGSVLQGHDHTLSVTLDDMIYHTQCVVRGSNKAFIMVDMPFGSSQVSPEETFVNAAR
                     LVSVGAQMVKIEGGQIMAETVDFLTRRGIPVCGHIGLMPQSVHQLGGYKVQGRTTNEA
                     EQLFNDAMVLQEAGAAMVVLETIPASLASKITRELSIPTIGIGAGIDCSAQVLVLHDM
                     LGIYPGNKPRFVKNFMSEASSIQAAIERYVEEVKTGQYPGAEHSF"
     gene            1977..2822
                     /gene="panC"
                     /locus_tag="PKZ49_06670"
     CDS             1977..2822
                     /gene="panC"
                     /locus_tag="PKZ49_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HND36282.1"
                     /translation="MEIISDISLLRTRLKPEQSIGFIPTMGNLHKGHLSLVTITQQHA
                     RCTVVSLFVNRLQFSPHEDFDRYPRTLQNDFALLKEAGVDIVFLPDEKTIYPAPQTFQ
                     LLLPALADTLEGAFRPGFFRGVTTVVLKLFNIVQPHVAVFGKKDYQQLHLVRDMVEQL
                     NLPITILAGETIRLKNGLAFSSRNSYLTSLERTEASQLYQSLNQIKHAITAGERNLTL
                     LEETAKQQLNQRNWKVDYLSVMKRNTLLPATPQDTELVILGAAWLGTTRLIDNLEVTL
                     PQPLG"
     gene            complement(2872..3696)
                     /locus_tag="PKZ49_06675"
     CDS             complement(2872..3696)
                     /locus_tag="PKZ49_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HND36283.1"
                     /translation="MTAFFEPLEYEFFCRGLLAALMVGALCGLIGVYVILRGMSYVGH
                     GLSHAAIGGAIVGFTLNLNFYAGACTMGLLAILIINKITKNNKIKLDAAIGIVTTAMF
                     ALGVAIVSKLRNFKQNFEAALFGNILGITDQDLIVITIVTVTAFIVLFFTYRALLFST
                     FDYEAAQVFGIKTGSVQLLFSLLLTFSVIASLNVVGVTMVAATLIAPAMTARMITDSF
                     SRMVIYSVLIGATTGVVGMYLSYFFNVASGTTIVLLSALLFCLSYLVKPLQRYRGG"
     gene            complement(3693..4514)
                     /locus_tag="PKZ49_06680"
     CDS             complement(3693..4514)
                     /locus_tag="PKZ49_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HND36284.1"
                     /translation="MSVIFPSNFQASPAIRLNQVTAGYDRNIVFSDLSLEIAPGQFAG
                     IVGPTACGKSTLLKIILGTHPVISGKIQLNKMPAGKVKPGTIGYVPQLESVDWNFPVT
                     VEEVIMMGLYTNGRIWPWLSKGERSRIHEIAERLGVDDCLNHHIIDISGGQRQRVFLA
                     RALISNPSLLILDEPTSGVDIKTQHEVLHLLSDLNREGITVVLTTHDLNAVASHLPWV
                     ICFNRGIVAQGRPYDIFTNEILTRTYGGDIVIVKYDGHLLIANSTPLNFIDVTRE"
     gene            complement(4492..5442)
                     /locus_tag="PKZ49_06685"
     CDS             complement(4492..5442)
                     /locus_tag="PKZ49_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HND36285.1"
                     /translation="MSRRFLMFLMLCCFQVAASAADQIARLKIATTVSPITNMVQNVG
                     GTTVEVKGVVPDGMDSHTFEPLPSDAKLLQNADLIIVNGLDLELPTLQLAEKVKKAHV
                     PILQLGNQAISQAEWHYDFSFPRELGHPNPHLWLNIELVMRYVQLIRDQLVVADPANK
                     ASYASNADIYLSKLNQLDQAIFKCVASIPEKNRKLVTYHDSFAYFAPRYGMTVIAAIQ
                     PHDFSEPGPQEVINIIKQIRSENVPAIFGSDVFPSRVMAQIAREAKVKFIDQLSDDEL
                     PPAPNHSFIGMMANNMVIMTEALGGDKTCMAGIDVSNISF"
     gene            5603..7672
                     /locus_tag="PKZ49_06690"
     CDS             5603..7672
                     /locus_tag="PKZ49_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HND36286.1"
                     /translation="MRYFIQIKAILYFTTALLLNAGFLPRSEAAGVPTLRAINVVANS
                     KELIGVANSANEGTVLKRQLDLRTIYRPGELLETVPGLIVTQHSGEGKANQYFLRGFN
                     LDHGTDLRISVDGMLVNQRSHGHGQGWADTNFIIPELTSGLQYQKGPYYADQGDFSSA
                     GAIHMGYADTLPKGILSYGIGQQGFMRGLIAKSHQVGHGNFLYATELLTKDGPWIKHE
                     DFNKFNSVLRYSQNDGPTKFNITAMGYGGKWNSTDQIPLRGVTSGFVPRLGAIDPTDG
                     GESHRFSLSSTLQHTSRLGVTHLNLYTVHSNLALFSNFTYFLDNPIQGDQFSQLDKRF
                     QSAFNIGHAWVNKLGPFDIENSVGMQFQNDIIDNGLLTTQQRHTLSTIRRDHINQNSL
                     GIYYQNSVQWLEKFRSVTGVRSDYYWFDVNSQLNVNSGNRFDSITNPKLSLIFGPWVQ
                     TEFYFNYGGGFHSNDARGTTATIDPKTGDSINRAHPLVRSTGYEVGVRTAVVPGLQAS
                     FALFRLDMDSELLFVGDAGTTVASRPSQREGFEISTFYMPTPWLMIDLDYALARAQFS
                     DNDPSGNHIPGAIRGVGKAAISIDNIGPVFGSLQFRYFGKRSLIEDNSVRSNHTMTLN
                     GQIGYKVSDNVRVIMQAFNLLNTKAHAIDYYYTSRLPGESLGGINDLHFHPIESRSFR
                     LNLVMNF"
     gene            7723..8184
                     /gene="nikR"
                     /locus_tag="PKZ49_06695"
     CDS             7723..8184
                     /gene="nikR"
                     /locus_tag="PKZ49_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HND36287.1"
                     /translation="MERFTISMDDQLFQQFDQAAQKRGYDNRSEAIRDLVRDYLETTR
                     LEKDIHGYCIATLSYIFNHHERDLASQVTSAHHHHHDITLSSMHVHMDHDNCLEVVIL
                     RGTIQDVRRFANRVIATNGVRHGKLHIVPIELSQEHHEHSSVPHTHSSPLT"
     gene            complement(8252..9235)
                     /gene="yut"
                     /locus_tag="PKZ49_06700"
     CDS             complement(8252..9235)
                     /gene="yut"
                     /locus_tag="PKZ49_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015204 - urea transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HND36288.1"
                     /translation="MGWSMAIDNAIPKPLRVMLRGVGQVFFCCNAVTGLIFLIALYVG
                     GITAGLAATVGVVSSTIAAYLLGFSDDDIEAGLYGFNGTLVGPCLFLFLENTPQLWLY
                     VVLASILSTVVLAALMRILQPYNIPASTSPFVLTCWMFMVAVYSFDGFSRGSLLPAPG
                     IPADIATTSAATMEAWYTNFSQATVEAWYTAITKGIGEVMFADSVIVGILFLVGIAIT
                     SLRGAVMALLGAIVGVAVPVLLGADKTLIELGLYAFNPVLTMMAVGWVFLKPSTGTAL
                     LALLAGILTIICQAGLANFLAPIGLPTLTFPFVLVMWMFLFAASKSKYWKS"
     gene            complement(9339..9974)
                     /gene="ureG"
                     /locus_tag="PKZ49_06705"
     CDS             complement(9339..9974)
                     /gene="ureG"
                     /locus_tag="PKZ49_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HND36289.1"
                     /translation="MNNVHKQSNPLRVGIGGPVGSGKTALCEALSKRMRDHYEMAVIT
                     NDIYTKEDMEILLRANALPPERLMGVETGGCPHTAIREDASINLEAIARMTHDFPNLD
                     LILVESGGDNLASTFSPELSDLTIYVIDVAAGEKIPRKGGPGITRSALLVINKIDLAP
                     YVGASLEIMERDAKKMRGDRPFVFTNMHTGEGVDQVVDFIVKQGLLDEVKH"
     gene            complement(9986..10663)
                     /locus_tag="PKZ49_06710"
     CDS             complement(9986..10663)
                     /locus_tag="PKZ49_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HND36290.1"
                     /translation="MQSLTLLRLLQLASPSLPIGAYTYSQGLESAIEKGTVSNESSAR
                     QWIAESLSIVADFEAPIVWRLLKAFTARDAEQVAYWTESFVAARDTAEFRAETIQMGY
                     SLGKLITDLNVADESLKAILANQSEVPLPTAFACAAVALGIPHEAALLGMLFSLVENQ
                     VLVCVKSVPLGQVSGQSLLLSLHPEIEHAAHHAQRLEDDELSNWAPGLSLLSMQHETQ
                     YSRVYRS"
     gene            complement(10672..11196)
                     /gene="ureE"
                     /locus_tag="PKZ49_06715"
     CDS             complement(10672..11196)
                     /gene="ureE"
                     /locus_tag="PKZ49_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HND36291.1"
                     /translation="MITLNTKIDHAETIYAQLVLPYEKRENSRLRATLASGEEVAIFT
                     ERGTVLRHNDLLKSADGQVVQIVAAHEPTYKITCRTPHDLLRCAFHLGNRHTQTQVGD
                     GFLRILQDTVLKQMLEGLGASIAEENAQFEPESGAYGGGGHHHHGDGHYHAHGPLAPI
                     PARQKIHRPSDTKA"
BASE COUNT         3391 a         2642 c         2315 g         3002 t
ORIGIN      
        1 catatcgcct tcattggatt gggcagtaat cttgaaaatc ctgttttgca aattaatcgg
       61 gcacttgctc atctagccag actttcagag atctgcattc taaaacaatc gtcactttac
      121 agcagcgccc caatcgatgc cccgaatcaa ccggattata ttaatgctgt ggtaaaagtt
      181 aaaacatcac ttcaaccgca ggcgttactc gcacaactct tagcgattga agaaaacagc
      241 gggcgtatac gtacttatcc caatgcagcg cgtatactgg atctagatct attgttatat
      301 gataacttgc aatatcgtga aaaaaatttg atagtccccc accccagaat gcatcaacgc
      361 gcttttgtac ttcgaccact cgtagaaatc gaaaacgata gcgttattcc tgggattggg
      421 ttagcttcag aattattaaa tcaatgtaat caacaaaaac tggaacgact cacataatca
      481 tgctgttagg taaataccgc tatatcgtca ttgaagggcc tatcggtgtt ggcaagtcca
      541 gccttgctca aaacatggct aactatttca actgtgccac cctattagag aaaccgaccg
      601 aaaacccttt tctcgaaaat ttctaccacg atattgctcg ttacgcatta cccactcaat
      661 taacctttct actgcagcga actgatcaat tcaagcaatt aatagaaacc aatctatcta
      721 ataaaccgtt aattggtgat tttttactgg aaaaggatca gctatttgcc aaactcacgt
      781 tatccagctc agaatatgcg atctatcaaa aaatttacca gcatatacag ccacaagcac
      841 ccaatcccga tttaatcatc tatttacagg cctcgcctga aatactcatt gaacgggtca
      901 agcgaagaaa tcatgcattt gagaaagcca tcacagaaga ttatctttgg aaattgacca
      961 atagttatac acaattcttc catcaatata aaaaagcccc tattttgatt gtgaatagtg
     1021 aaaatctcaa ttttgctaac aacaccaagg attttaattt actacttcag caaattagtc
     1081 aaatgcgtgg tgcaatagaa tattttagtt gtagcgaaat ccattaatta gaagaacata
     1141 tgagaacaac acgagacaca ttacaaagaa tgtacgcgga aggtaaaaaa atagcggtgc
     1201 tcacttgcta tgatgcgacc tttgcccact tacttgagaa tgtaggtatc gatattttgc
     1261 tgattgggga ctcactcggc agtgttttac aaggacatga ccatacatta tcggtcacct
     1321 tggatgatat gatttaccat acccaatgcg tcgtgcgcgg ctcaaataaa gcattcatta
     1381 tggttgatat gcccttcggc tcttcgcaag tctctcctga agaaactttc gtcaacgcgg
     1441 ctcgtttagt atcggttggt gcgcaaatgg tgaaaattga aggtgggcaa attatggctg
     1501 aaaccgttga ttttctgact cgtcgtggca ttcccgtttg tgggcatatc ggtttgatgc
     1561 cacaaagtgt ccaccagctc ggtggctata aagttcaagg tagaacaaca aatgaagcag
     1621 agcaattatt caatgatgcg atggtattgc aagaagccgg agcagcaatg gtagtgttag
     1681 aaacgatccc ggcttcgctt gctagtaaaa tcacacgtga actatcgatt cctaccattg
     1741 ggatcggtgc aggcatcgat tgctcggccc aagtactcgt cctacacgat atgcttggaa
     1801 tctaccccgg aaacaaacct cgcttcgtta aaaattttat gagtgaagca agcagtatac
     1861 aagcggccat tgaacgctac gttgaagaag tgaaaactgg ccaatatcca ggtgctgagc
     1921 atagcttcta aaaactaaag cgctatttgc tcgcatttct ttgatcgcca atcctgatgg
     1981 aaataatttc cgatatttcg ctactcagaa caagacttaa gcctgagcaa tcgattggat
     2041 tcattcctac catggggaat ttacacaaag gacacttatc actcgtcacc attacacaac
     2101 agcatgcacg ctgtacagta gtcagtttat ttgtaaatcg cttacaattc tcgcctcacg
     2161 aagattttga tcgatatccc cgtaccttac agaatgattt tgcgctacta aaagaagcag
     2221 gggttgatat tgtttttcta cccgatgaaa aaacgatcta ccccgcaccc caaacatttc
     2281 aactgctact tcccgcgctg gccgatacct tagaaggggc gttccgtcct ggttttttta
     2341 gaggggttac gactgtcgta ctcaagttat tcaacattgt tcaaccccat gttgccgtat
     2401 ttggtaagaa agattatcag cagttacatc tggttcgtga tatggttgaa caattgaatc
     2461 taccgataac gatcttagcc ggtgaaacaa tccggctaaa aaatgggctt gcgttcagct
     2521 cccggaatag ctatctaaca tccttagaac gcactgaagc aagccagctt tatcaaagct
     2581 taaatcaaat caagcacgcg attaccgcag gggaacgtaa cttaacgtta ctagaagaaa
     2641 ccgctaaaca gcaactcaat caacgtaatt ggaaagtgga ttatctctca gtgatgaaac
     2701 gcaataccct cttgccagcc acgcctcagg atactgagct cgttattctc ggggcagctt
     2761 ggttaggtac aacacggttg attgataacc ttgaagtcac cctcccccaa ccacttggtt
     2821 aatacccatt tctaatattg acgcgaatct aatatttttt gatcaatggc ttcatccacc
     2881 tcgatatctt tgcaagggtt taacaagata agataagcaa aataacaacg cactcaataa
     2941 aacaatcgtt gtgcccgaag caacattgaa aaaataactc aaatacatac ccactactcc
     3001 agtggtagca ccgatcaaga cggaatagat caccatgcga ctaaaactat ccgtaatcat
     3061 tctcgccgtc atagccggtg caatcaaagt tgccgccacc attgttacgc caactacatt
     3121 caatgaagca atcacagaaa atgttaatag taatgaaaac agcagttgca ccgaccccgt
     3181 tttaatacca aacacttgcg ctgcttcata atcaaatgtc gagaaaagga gtgcacgata
     3241 ggtaaaaaac aaaacgataa atgctgtcac tgtgacaatc gtgatcacga ttaaatcttg
     3301 atcggtaatg cccaaaatat tgccaaacaa ggcagcttca aaattttgct taaaattacg
     3361 gagtttactg acgatcgcca cacccagcgc aaacatagcg gtagttacaa tcccaatcgc
     3421 cgcatccaat ttaattttgt tatttttagt aatcttatta atgatcagta tcgccagcaa
     3481 acccattgta caagcgcctg cataaaaatt cagattaagc gtgaagccga caatggcacc
     3541 cccaattgcc gcatgcgata agccgtgacc tacataactc ataccacgca aaatgacata
     3601 gacaccgatt aatccacata aagcacctac cataagggct gcaagcaaac ctcggcaaaa
     3661 gaactcatac tccagtggct caaaaaaagc tgtcattcac gagtcacatc aataaaattc
     3721 aggggggtac tattggcaat caacaaatgc ccatcatatt tgacaatgac gatatcgccg
     3781 ccataagtcc gcgttaagat ttcattcgtg aaaatatcgt aggggcgacc ctgtgcgaca
     3841 atgcctcgat taaaacaaat gacccaaggt agatgagaag caaccgcatt taaatcatgt
     3901 gtcgtcaaga cgacggtgat accctctcga ttaagatcac tcagcaaatg caacacttca
     3961 tgctgtgttt taatatcgac gccagaagta ggctcatcca aaatcaaaag gctgggatta
     4021 ctaatcaagg cacgtgccag aaaaacccgt tgtcgctgcc cccctgaaat atcgataata
     4081 tggtggttca ggcaatcgtc gacacctaat ctctcggcga tctcatgaat acgactgcgt
     4141 tcccctttac tcagccaggg ccaaatacga ccattggtat aaagtcccat catgatgact
     4201 tcttcaacag tcacggggaa attccaatca acgctttcta gctggggcac ataaccaatg
     4261 gtgccaggct ttactttacc ggctggcatt ttattcaatt ggattttgcc cgaaatcacc
     4321 ggatgcgttc ctaaaatgat tttcagcagc gtactctttc cacacgccgt aggacctaca
     4381 atcccagcga attgccctgg cgctatttca agcgaaagat cagaaaaaac gatattgcgg
     4441 tcatagcccg cagtaacttg attgagccga atggctgggc ttgcttggaa attagaagga
     4501 aatattactg acatcgattc cagccataca cgtcttatcc cctcccaggg cttctgtcat
     4561 gatcaccata ttattggcca tcataccgat aaatgaatga ttgggtgcgg ggggtagctc
     4621 atcatcggaa agctgatcaa taaatttgac ctttgcttct cgcgcaattt gggccattac
     4681 tctgcttgga aacacatcag aaccaaatat cgccggtaca ttctcagatc gaatctgctt
     4741 aatgatatta attacttctt gtggacctgg ctcagaaaaa tcgtgcggtt gaattgccgc
     4801 aatcactgtc attccatagc gcggcgcaaa atacgcaaat gaatcgtggt aagtcaccag
     4861 cttgcgattc ttttcaggaa tcgatgcgac acacttaaat attgcctggt ctaactgatt
     4921 aagcttgctc aaatagatat cagcattgct ggcataactc gctttatttg ctggatcagc
     4981 aacaacgagc tggtctcgaa tgagctgaac ataacgcatg accaattcga tgtttaacca
     5041 taagtgtggg ttaggatggc caagctcgcg tggaaaacta aaatcataat gccattccgc
     5101 ttggcttatc gcctgattac cgagctgtaa aatgggcaca tgggcttttt tcactttctc
     5161 ggccagctgt aaagtcggaa gctccagatc gaggccattg acgatgatca aatccgcatt
     5221 ctgtaatagc ttggcatccg aaggaagcgg ttcaaaggta tgggaatcca tcccatccgg
     5281 cacgactcct tttacctcaa cagtcgtacc gccaacattt tgcaccatgt tggtaatggg
     5341 agaaactgtc gtcgcaatct ttagtcttgc aatctgatca gcagcgctgg ctgcaacttg
     5401 aaagcagcat agcattagaa acattagaaa tcgtcgtgac atagtgcccc taaaattgcc
     5461 tatcaatccc tacgctattt tgatccaaat caaaaaagta tgaaagtaaa tttattcttc
     5521 atacattata agttagaatt cagctagatt tcacatagat ttcgcagttt ttatactaaa
     5581 taacaaaaat cctgagtgta aattgcgata ttttattcaa ataaaagcaa ttttgtattt
     5641 cactactgcc ttgctgctca atgcggggtt tcttcccaga agcgaggcag ccggtgtgcc
     5701 gacacttcgg gcaatcaatg ttgtcgctaa ctcgaaagaa ttaatcggtg tcgcaaattc
     5761 agccaatgaa ggaaccgtac tcaagcgtca acttgatttg cggacgattt atcgacctgg
     5821 agagctactt gagacagtac ccggcttgat cgtgacgcaa cacagtggcg aaggcaaagc
     5881 caatcagtat tttttgcgtg gctttaactt agaccatgga acggatctac gtatttcggt
     5941 tgatggcatg ctagtcaatc agcgttcaca tgggcatggc caaggttggg ctgataccaa
     6001 ttttattatt cccgagctaa ccagcggctt acaatatcag aaagggcctt attatgcaga
     6061 tcaaggtgat ttttcttccg ccggggcgat tcatatgggc tacgctgata ctcttcccaa
     6121 aggtattcta agctatggca ttggacagca aggcttcatg cgcggcctga tcgccaaatc
     6181 ccatcaagtc ggtcatggaa attttctcta tgcgaccgaa ttgctgacca aagatggtcc
     6241 ttggatcaag catgaagatt ttaataagtt caattcggtc cttcgctata gtcagaacga
     6301 tggtccaacc aaattcaata tcactgcgat gggttatggt ggaaaatgga actcgaccga
     6361 tcagattcca ctccgtggcg tgaccagcgg attcgtgcct agattgggtg cgatcgatcc
     6421 caccgatggg ggtgaatccc atcgttttag cctctctagc acgctacaac atacaagtcg
     6481 attgggtgtc acgcatctca atttgtatac cgtgcattcc aatctagcat tgttttcgaa
     6541 tttcacctat tttcttgata atccgattca gggtgatcaa ttttctcagc ttgataaacg
     6601 ctttcagtca gcgttcaaca taggacacgc atgggttaat aaattgggtc cgttcgatat
     6661 cgaaaatagc gtcggcatgc aatttcaaaa tgatatcatc gacaatggat tgctcaccac
     6721 gcaacagcgg catacgttat cgaccatccg tcgagatcat atcaaccaga atagcttggg
     6781 tatctattat cagaatagtg tgcaatggct cgagaaattt agaagcgtaa ccggtgtccg
     6841 ctccgactat tactggtttg atgtgaacag tcagttaaac gtgaattcgg gcaatcgctt
     6901 tgatagcatc accaacccta aactgagttt aatattcggc ccatgggttc aaactgaatt
     6961 ctatttcaat tacgggggag gctttcatag caatgatgca cgcggaacaa cagcaaccat
     7021 tgaccctaaa acaggagatt ccattaaccg agcccatcca ttggtgcgct ccacagggta
     7081 tgaagtaggc gtacgaacag cggtagttcc aggattgcaa gcttcatttg cactcttccg
     7141 actagatatg gattctgaat tattatttgt cggcgatgca ggtacaaccg tggccagcag
     7201 acccagtcaa cgcgaagggt ttgaaatttc gaccttttat atgccaactc cttggctaat
     7261 gatcgatctc gattatgcgt tggccagagc acagtttagt gataacgacc ccagtggtaa
     7321 tcacattcca ggggccattc ggggagtcgg aaaagcagct atctcgatcg ataatatcgg
     7381 gcctgttttt ggtagtctgc aatttcgcta ttttggcaaa cggtcgctca tcgaagataa
     7441 tagcgtacgc tcaaaccaca cgatgacgtt gaatggtcaa atcggttata aagttagcga
     7501 taatgttcgc gtaatcatgc aagcctttaa tttattgaat accaaagcgc atgccatcga
     7561 ttattactat acttctcgac taccaggaga atcgttaggg ggaatcaatg atttacattt
     7621 tcatccgatc gaatctcgat catttcgcct taatctagtg atgaattttt aattttaccg
     7681 cttattacta attgttatgt gtccattcag gagaaattaa ttatggaacg ctttacgatt
     7741 tcaatggatg atcagctctt ccaacagttt gatcaggctg ctcaaaaacg aggctatgat
     7801 aatcgatcag aagccattcg cgatctggtt cgtgattatc tggaaacaac tcggttagaa
     7861 aaggatattc atggctactg catcgcaaca ttgagttaca tttttaatca tcatgagcgg
     7921 gatttagcca gtcaagtaac ctcagcacac catcaccatc acgatattac cttatcgagc
     7981 atgcacgttc atatggacca tgataattgt ctagaagtcg tgattctcag gggtacgatc
     8041 caagatgtca ggcggtttgc aaaccgagtc atcgctacca atggggtacg gcatggaaag
     8101 ctacatatcg taccgataga actctcccag gaacatcatg aacattcttc ggttccacac
     8161 actcacagta gcccattgac ctaatcatat tcaaccaata aaaaaacccg ccagaaggcg
     8221 ggtcattcaa tgaattcagt tgaagctaga ttcaactttt ccagtatttc gatttactag
     8281 cagcaaataa aaacatccac attaccaaaa caaaaggaaa ggtaagggtt ggtaatccaa
     8341 tcggtgcaag aaaatttgct aatccagctt gacaaataat cgtcaagatt ccagctaaca
     8401 aagccagaag cgctgttcca gtagagggtt tgagaaaaac ccaacccaca gccatcatgg
     8461 tcagaacagg attaaatgca tataaaccca gttcaatcaa ggttttatcc gcacccagta
     8521 agactggtac tgcaacaccg acaatggcac ctaataatgc catcactgca ccacgcagcg
     8581 aagttattgc gataccaacc aggaatagaa tacccacaat gacactgtca gcaaacatca
     8641 cttcccctat ccccttggta atcgctgtat accaggcttc aacggttgct tgactaaagt
     8701 tggtatacca ggcctccatg gttgctgcgg aggtagttgc gatatctgct ggaattccag
     8761 gcgctggtag taaagaaccc cgagaaaagc catcaaaaga ataaaccgca accatgaaca
     8821 tccagcatgt caggacaaac ggagatgttg aagcaggaat attgtacggc tgcaaaatac
     8881 gcattagtgc tgccaacacc acggtggaca gaatcgatgc aagcacgaca tacagccaaa
     8941 gttgaggtgt gttctcaagg aataagaaca agcagggccc aacgagcgtt ccattgaaac
     9001 cataaagccc agcttcgata tcatcatcag aaaaacctaa taaataggct gcaatcgtac
     9061 tactaaccac accaaccgtt gccgccaggc ctgcagtaat tccgcctaca tacagtgcga
     9121 tcaagaaaat aagcccggta accgcattgc agcaaaaaaa gacttgccca acccctctga
     9181 gcattactct cagtggtttt ggaattgcat tgtcaatagc catagaccat cccatagtga
     9241 ctaactcctc aagtagtatt ttgtaaaata aaactgcgat aactcgacta gaaccttaac
     9301 tcagcttact cagttgtatt gaatttctac atagtagatt aatgcttcac ttcatccagc
     9361 aaaccttgct tcacaatgaa atcgacaact tgatcaacac cctcccctgt atgcatattg
     9421 gtaaacacaa aaggacgatc tcctcgcatt ttcttagcat cacgttccat aatttctaga
     9481 ctagccccaa cataaggcgc caaatcgatt ttattgatga ccagtaaagc agagcgtgtg
     9541 ataccaggcc cccctttgcg gggtattttt tcgccggcgg caacatcgat gacgtagatg
     9601 gttaaatccg ataactcagg actaaacgtt gaagccaggt tatctccacc cgattcaact
     9661 aagattagat cgaggtttgg aaaatcatgc gtcatgcgcg cgattgcctc taaattaatc
     9721 gatgcatctt ctcgaatcgc cgtatgcgga catccgcctg tttctacgcc cattaagcgt
     9781 tccgggggta aggcattggc gcggagcaaa atctccatat cctctttggt atagatatca
     9841 ttggtgatga cggccatttc ataatggtct cgcatgcgtt tgcttaacgc ttcacatagc
     9901 gctgttttac ccgatccaac cgggccgcct attccaactc gcagtgggtt tgattgtttg
     9961 tgtacgttgt tcatagtgat atagattaag atcgataaac tcggctatat tgcgtttcgt
    10021 gttgcatgga aagcaatgaa agtccaggcg cccaatttga tagctcatca tcctcaagtc
    10081 gttgtgcatg atgggcagca tgttcaatct ctgggtgcaa tgacaggaga aggctctgcc
    10141 cagaaacctg tccaagcggt actgatttga cgcaaacgag tacttgattt tcaaccaaag
    10201 aaaatagcat acccaataac gccgcttcat gcggaatacc gagtgcaaca gcagcacaag
    10261 caaacgctgt aggtaaaggt acttctgatt gattcgccag tatagctttt aaagattcat
    10321 cagcaacatt aagatccgtg atcaacttac cgagggaata gcccatttgt atcgtttctg
    10381 cacgaaattc agccgtatct ctcgctgcaa caaaactttc ggtccagtag gcaacttgct
    10441 ccgcatcacg ggcagtaaat gccttgagta gacgccacac gatgggtgct tcaaaatcag
    10501 ccacaatact cagcgattca gcaatccatt gacgtgcaga gctctcattg gacaccgttc
    10561 ccttctcaat cgctgattcc agtccttgtg aataggtata ggcaccgatg ggaagcgatg
    10621 ggctcgccaa ctgaagcaga cgcaatagtg ttagagattg caaaatagct cctatgcttt
    10681 ggtatcagat ggtcgatgaa tcttttgtcg cgcaggaata ggggctaatg gaccatgtgc
    10741 atgataatgg ccgtcaccat gatggtgatg accgccaccc ccataagcac ctgattcagg
    10801 ctcaaattgt gcattctctt cggcaatcga ggcgcccagc ccctcaagca tttgctttaa
    10861 taccgtatct tggagaatac gtagaaagcc atcaccgact tgtgtctgtg tatggcgatt
    10921 ccccaaatgg aatgcgcaac gtaataaatc atggggcgtg cggcaggtta ttttataagt
    10981 aggctcatgg gcagcaacaa tctgaaccac ttgcccatca gcacttttga gtaaatcatt
    11041 atgacgaaga acggtgccac gttccgtaaa aatggctact tcttctccgg aagcaagcgt
    11101 agctcttaat cgactattct cacgcttttc ataaggtaga accaattgag cgtaaatagt
    11161 ctcagcatga tcgattttag tatttaatgt aatcatctgt tttattctgg tttatagata
    11221 agttatagat gattactatc acagcaatct aggtattaac gacggctgtg atagtcactg
    11281 ccaaatagcg cagtccatta cgctatgact aaacctaata ggttagctaa tgcatgaact
    11341 gcgcaacttg
//
LOCUS       DAOQKQ010000080        11291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_2694_length_11291_cov_5.305091, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000080 DAOQKQ010000000
VERSION     DAOQKQ010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 11291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11291
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2694_length_11291_cov_5.305091"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            299..1246
                     /gene="pstC"
                     /locus_tag="PKZ49_06720"
     CDS             299..1246
                     /gene="pstC"
                     /locus_tag="PKZ49_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HND36292.1"
                     /translation="MSSKFTKDTGVDPGYSKGSLKYSYKRHLKEKIIELLLLLAAVFS
                     VLVTFAIIYMLVKESYVFFQHVSLKEFLTDTQWTPLFDDAHYGILPLVSGTLVSSVVA
                     LMVALPLGTIIAIYLSEFASFRVREIAKPFLELLGGIPTVVYGYFALLFVTPLLQKIF
                     PNLPGFSLLSAGLVMGIMIIPYVSSMAEDAMRAVPMNLREGSYAMGATRFQTAINVVT
                     PAALSGIAAAYILGISRAVGETMVVAIAAGMQPNLTWNPMDQAATITAYIVQVSLGDL
                     PHGSIGYQTIFAAGLTLLLITLIFNILGHLLRKYYRESY"
     gene            1319..2251
                     /gene="pstA"
                     /locus_tag="PKZ49_06725"
     CDS             1319..2251
                     /gene="pstA"
                     /locus_tag="PKZ49_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HND36293.1"
                     /translation="MIEENQTDLRELRSIIVRHKRWDLIFLICGVLALMIAILTFIAL
                     FLNMLIDGLPRLSWEFFTSYPSRRAESAGILSAWVGTTLVMLVTAVSAVPLGIAAGVY
                     LEEYAPKNFFTELIEINVTNLAGVPSIIYGLLALGLFVYQLGLGQSILAAGLTLALLI
                     LPIVIVTTREAIRSIPVAIREGSYALGATKWQTVADHLLPYSAPSILTGIIIGLARAI
                     GESAPIITIGALTFIAFLPNAPVTHEFPFVSFEWLLAPFTVMPIQMFNWVSRPEEAFQ
                     FNAAAAGLVLVIMTLTMNALAIYLRYQMRKNIKW"
     gene            2291..3058
                     /gene="pstB"
                     /locus_tag="PKZ49_06730"
     CDS             2291..3058
                     /gene="pstB"
                     /locus_tag="PKZ49_06730"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HND36294.1"
                     /translation="MKNQHKSVVKNLSFHYGDFNALKNIDMVLHDKQVTALIGPSGCG
                     KSTFLRCFNRMHDLYPGNRYEGNIILYPDNENILAPSVDPIEVRMRISMVFQKPNPFP
                     KSIYENVAYGLRIRGIRQRALLDEKIEEALRNAALWDEVKDRLHELAFNLSGGQQQRL
                     CIARALATNPEILLFDEPTSALDPIATASIEELIADLKSKVTILIVTHNMQQAARVSD
                     YTAYMYLGQLIEFDLTDTIFIKPKNKQTEDYITGRFG"
     gene            complement(3068..3793)
                     /locus_tag="PKZ49_06735"
     CDS             complement(3068..3793)
                     /locus_tag="PKZ49_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HND36295.1"
                     /translation="MPDHSFKAMILAAGRGERMRPLTDTIPKPLLRVGNKMLIEYHLE
                     NLVLAGFKEIVINHAYLGDMIEKALGDGARYGVAIQYSKETIKLETAGGIANALPYLT
                     ADHRNQPFLVVNGDIYCPIDFSSLIPDLARLNAQSNDDLAHLLLVNNPSHHPEGDFYL
                     SEQGRIIATGQTKLTFSGIGIYRPQLFEQVAINCPTRLAPLLHQAVANHQVSGKFFSG
                     KWVDIGTPDRLNALDQQLRSQQR"
     gene            complement(3801..4811)
                     /locus_tag="PKZ49_06740"
     CDS             complement(3801..4811)
                     /locus_tag="PKZ49_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HND36296.1"
                     /translation="METDRLHQLKAWLHNEFPNQHFDLSIASADASFRRYFRVQFEDR
                     SLIAMDAPPQHEDCRPFIKIAQLFSTADVNVPSILSQNLTDGFLLLTDLGNTTYLQQL
                     VAHPSEARAMYSDAIDALIRMQQISQENILLNYDRALLQKELNLFPEWYLTRHKQFNL
                     SQHQSDHLQSTFSAILANNLEQPKVFVHRDFHSRNLMITTPNPGILDFQDAVYGPITY
                     DLVSLFKDAYIQWEEELVLDWVIRYWEKARKARLPVSSDFSIFYRDFEWMGVQRHLKV
                     LGIFARLYHRDNKAGYLNDIPLVLHYLRKTCERYRELHLLKTLLDEIEDAPSPQQVGY
                     TF"
     gene            complement(4921..5568)
                     /locus_tag="PKZ49_06745"
     CDS             complement(4921..5568)
                     /locus_tag="PKZ49_06745"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HND36297.1"
                     /translation="METKQLESHFKRSLIAAAASLLLIGCGAEQGKESFSDRVAKRFG
                     GGDAAGLPEPAWKEAAAEHKKAKEAGAASGDKGKEPEKKEPEKAAAEPAKPSPVEATI
                     QATEEIAADVGEKISAVAEDIKTEVSESVNSLTAMATPSADSPTEDLKAVVKPTPEII
                     RQLQQALTDAGYNPGPVDGKSGGKTTEAMKKFQKDNNLTVGQITKETLATLGIAY"
     gene            6929..8503
                     /locus_tag="PKZ49_06750"
     CDS             6929..8503
                     /locus_tag="PKZ49_06750"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HND36298.1"
                     /translation="MSNYLQRDESKQIQLSGWRCLLPWLLSSFCLLLASVSHQFASAQ
                     EIDSLRKGVVKVAATLDGIQKTGTEFIVQQDTKATYIVTAAHVVADDKYPKVAFFSER
                     HTFVETEVLQNDQRNDTSLLEVHDNTLVSTDVIALLFAAEEKVQDNNELIAIGFTPGV
                     EWAQTPVTLALRQATDLILDGNLDEGNSGGPILKKGSINDLVTKGDNRLGRAIPEKFI
                     TTVLDGWDITLMKGGVVTAQFTSKPTPHEKPPTTTRLPIEPDMVAVPAGKLIMGSPKT
                     EPGRYSNEGPQHEVFIASFAISRTEITVGQFRLFVQDKDYHGGKEFQTAAEENEKGCW
                     TWNEEKKQFERIPQRNWKSPGFHQHDEHPAVCVSWDDAQAYVQWLLKKTGKQYRLPTE
                     AEWEYVARAGTTTVYWWGDNIGKNNAVCGDCGSQWDGRQTAPVGSFKPNAFGVYDTAG
                     NVWEWTQDCWHDDYTNAPTNGTAWLEKDNGDCSRRVVRGGSWGFDPLDLRSAGRDWII
                     TDGAGYGTGFRVARDF"
     gene            complement(8606..>11291)
                     /gene="uca"
                     /locus_tag="PKZ49_06755"
     CDS             complement(8606..>11291)
                     /gene="uca"
                     /locus_tag="PKZ49_06755"
                     /EC_number="6.3.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004847 - urea carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urea carboxylase"
                     /protein_id="HND36299.1"
                     /translation="DLVEWMVKQAAGELPPLTAFTIKPQGVAIQARIYAENAAKNFQP
                     CCGTLTTVEFPQTCRVETWVENGTELSAFYDPMIAKMIVHAPTRNAAVNKLITALNET
                     SLYGIETNLSYLKQIISSDVFQKGQVSTQFLSQFTYLANTLDVLDPGTQTTVQDYPGR
                     LGYWNVGVPPSGPMDSLAFRLANRLVNNPEHLAGLEITLNGPTLRFNCASVIALCGAP
                     IEALLDNEPLALWQSHAVRAGTLLKISKIDSVGSRVYLAVQGGIQVPHYLGSRSTFML
                     GQFGGHAGRALQSGDVLSIKPAKPFVAQKLREAIIPRYTHDWEIFVLYGPHGAPDFFT
                     QADIEQFFATAWKVHHHSSRTGVRLVGPKPQWARPDGGEAGLHPSNIHDNAYAIGTID
                     FTGDMPIILGPDGPSLGGFVCPATIIAAELWKIGQLRPGDRIRFRPLSLENAQMLETQ
                     QNHLIEHLDHAQPAATSSIAEDWGQPILQQIPVQANQVQVTYRQAGDKYLLVEYGPPI
                     LDLNLRFRIHALMTLIEAQAQGIIDLTPGIRSLQIHFDTRIRTRDELLAWLMACESRL
                     PALDDMNIPSRIIHLPLSWDDPATQIAIEKYNRVVRPAAPWYPSNIEFIRRINGLDHI
                     DDVKQIVFDASYLVMGLGDVYLGAPVATPLDPRHRLVTTKYNPARTWTPENAVGIGGA
                     YLCIYGMEGPGGYQLVGRTLQMWNRYRQTAAFKTHRPWLLRYFDQIRFYPVSEAELLK
                     HRADFIAGRLKLRIEESTLNLKQYNAFLQQHRESIESFKTQQQKAFDEESQRWKATAA
                     FETVNEDILEAADAQIEIDLPAGAEIITAPVSGTVWKLLIEAGQQINQQEPILVIESM
                     KMEFPVPAPADGVVVQLFCQPGNYVAAGQALGVLQHSC"
BASE COUNT         2987 a         2384 c         2691 g         3229 t
ORIGIN      
        1 gaagctaaat ctgaagcttc cgatgatgat gaagatgacg aggatgagta aggttggctt
       61 aatccaattc tagcatctgt tgtatcaaag acgaggttct ctgatgacag gaacctcgtt
      121 aaaggttcaa agaaattgaa gaataatctt cgcttctata ggagctccat cctaatctga
      181 gcaatgatca ggttgtcgag gaaggtgctt atttcacaag cttgtaacaa taagcgccta
      241 ctctagctta cataataaaa atatgaattg gctttcccac taacggtaaa tcgataagat
      301 gagttctaaa tttacaaagg atacgggtgt cgatccgggt tattctaagg ggagtctcaa
      361 gtatagttat aagcgacatc tcaaagaaaa gatcattgaa ttactgctat tacttgccgc
      421 ggtcttttcc gttcttgtca cgtttgcaat catttacatg ctggtaaaag aatcctatgt
      481 tttttttcag catgtatcat taaaagaatt tctgacggat acgcagtgga ctcctttatt
      541 tgatgatgct cactatggca tacttccact cgtttctggc acgctggtaa gttctgttgt
      601 cgcactcatg gttgctctgc cgcttggaac catcattgca atctatctgt ctgaatttgc
      661 ttcatttaga gtgcgtgaaa ttgctaaacc atttcttgag ttactaggtg gcattccaac
      721 tgtggtttat ggttattttg cgctactgtt tgtaacaccg ctgcttcaaa agatttttcc
      781 taatttacct ggattcagct tgctatcagc cggtttggtg atgggaatca tgattattcc
      841 atatgtgagt tccatggctg aggatgcgat gcgagcagtt ccaatgaatt tgcgtgaggg
      901 atcttatgcg atgggagcaa cacgcttcca aacggcaatt aatgtcgtca caccggcagc
      961 tttatcaggg atagcagcag cctacatatt aggtatttcc agagcagtag gtgaaaccat
     1021 ggttgtggca atcgctgcag gtatgcaacc taatctgact tggaatccta tggaccaagc
     1081 tgcaacgata actgcgtata tcgtacaggt aagtttaggg gacttgccac atggcagtat
     1141 cggttaccaa acaatttttg ctgcaggctt aacgttgcta ctcattacac tcattttcaa
     1201 tattctaggc catttattaa gaaaatatta tcgtgagtct tattaagagt gagttaataa
     1261 atgagtccca gaatagcatc ttgtcgagtg taacgtgttt ttctaattta gtagggttat
     1321 gatagaagaa aaccaaactg atctaagaga attaagatca atcattgttc ggcataaacg
     1381 ctgggatttg atttttctga tctgtggtgt gctcgcgttg atgattgcta ttctgacctt
     1441 tatagccctt tttctgaata tgctcattga tggcttgcca cggttatcct gggagttttt
     1501 tacgtcttac ccttcacggc gggctgaatc ggcgggtatt ttgtcagctt gggtaggtac
     1561 aactttggta atgcttgtga ctgctgtttc agccgttcca ttagggatcg ccgcaggcgt
     1621 ttatttagag gaatacgccc ccaaaaactt ctttacagaa ttaattgaaa ttaatgtgac
     1681 caatcttgca ggcgtaccct cgatcattta cgggttattg gcacttggac tttttgttta
     1741 tcaattaggt ttggggcaaa gcattttagc tgcaggatta acactcgcat tactgatttt
     1801 gccaattgtt atcgtgacaa cccgtgaggc gatacgctct attcctgtag cgattcgtga
     1861 aggatcctat gctctcggcg caacgaaatg gcaaaccgtt gctgaccatt tattgcctta
     1921 ttctgcgcca agtattttaa ccggtattat cattgggctc gccagagcga tcggtgaatc
     1981 tgctcccatt attaccatcg gtgcattgac ttttatcgct tttttaccta acgccccagt
     2041 aacgcatgaa tttccctttg tttcatttga atggttactc gcgcctttta cagttatgcc
     2101 aatccaaatg tttaattggg tatcgaggcc tgaagaagca tttcaattca atgcagccgc
     2161 tgcagggctg gttttagtca ttatgacgct gactatgaat gcactcgcca tttatttgcg
     2221 ctatcagatg cgtaagaata ttaaatggtg acagaaaata ttcaactgat aataataata
     2281 cgaacctctt atgaagaatc agcataaatc ggtagtaaag aatttaagct ttcactatgg
     2341 cgactttaat gcattaaaaa atatcgatat ggttttgcat gataagcaag taactgcttt
     2401 gattgggcca tccggttgtg gaaaatcgac ttttctccgg tgttttaatc ggatgcatga
     2461 tctttatcca ggaaatcgct atgaaggtaa tatcatctta tatcctgata atgagaatat
     2521 ccttgcaccg agcgtcgatc cgattgaagt gaggatgcgt atcagtatgg ttttccagaa
     2581 acccaatcca tttcccaaat ccatttatga aaatgtagcc tatgggctca ggataagagg
     2641 aattcggcag cgtgcgctac tggatgagaa aatcgaagaa gcgcttcgca atgctgcatt
     2701 atgggatgaa gttaaagatc gtctccatga attagccttt aatttatccg gtggacaaca
     2761 gcaaagactt tgcattgcgc gagcgctggc aaccaatcca gaaattttac tgtttgatga
     2821 accaacctcc gcgcttgatc cgattgcaac cgcaagtatt gaagaattaa ttgccgatct
     2881 aaaaagtaaa gtgacgattc tgatcgttac gcataacatg cagcaggctg cccgcgtatc
     2941 ggactatacc gcttatatgt atttaggcca attaatcgag tttgacctga ctgatacgat
     3001 ttttattaaa cctaaaaaca agcaaactga agattatatt accggtcgat ttggttaata
     3061 tgcttgacta gcgttgctgg gatcgaagtt gttgatccag cgcgtttaga cgatcgggtg
     3121 taccgatatc aacccatttt cccgagaaaa acttaccact cacttgatga ttagcaactg
     3181 cctgatgcaa gagtggcgct aatcgtgttg ggcaattgat agctacctgt tcaaacagtt
     3241 ggggtcggta gattccaatg ccactaaatg taagtttcgt ttgaccggtt gcgatgatgc
     3301 gaccttgctc agatagataa aaatcacctt ctggatgatg agaaggatta ttaacgagca
     3361 agaggtgtgc gagatcgtca tttgattgtg cattcaaacg cgctaaatcg ggtatcagcg
     3421 atgagaaatc gatagggcaa tagatatcgc cgttgacgac taaaaaaggt tggttgcgat
     3481 gatcagcagt gagatagggt agtgcatttg cgataccgcc ggctgtttca agtttaatgg
     3541 tttcttttga atattggatg gcgacaccgt aacgtgcgcc atctcccagt gctttttcaa
     3601 tcatatcgcc aagatacgca tgattgataa cgatttcttt gaagccagcg agtacgagat
     3661 tttcaagatg atactcgatc aacattttgt ttcccacgcg aagcaacggt ttgggaatag
     3721 tatccgtgag cgggcgcata cgttcacccc ggcctgctgc tagaatcatt gccttaaaag
     3781 agtgatcagg catacaacga ctaaaaggta taacctactt gttgtggcga tggcgcatct
     3841 tcaatttcat cgagcaaagt tttcaatagg tggagctcac ggtagcgctc acaggttttg
     3901 cgtaaataat gcaagacgag aggaatatca ttgagataac ctgctttgtt atcgcggtga
     3961 taaaggcgtg caaagatacc taataccttc aagtgacgct gtacgcccat ccattcaaag
     4021 tctcgataga agattgagaa atctgatgaa acgggtagcc ttgcttttcg tgctttttcc
     4081 cagtagcgaa tcacccaatc aaggactaac tcctcttccc actgaatgta ggcatcttta
     4141 aacaaggaaa cgaggtcata ggtaatgggg ccatagaccg catcctgaaa atcgagaata
     4201 ccaggattcg gtgtggtaat catgagatta cgcgaatgga agtcgcggtg cacaaatacc
     4261 ttgggttgtt cgagattatt ggcgagtatt gctgagaaag tcgattgcag atgatctgat
     4321 tgatgctgac tcagattgaa ctgtttgtgt cgcgttaggt accattcggg aaatagatta
     4381 agctcctttt gtagcagggc gcgatcataa ttcagcaaga tattttcctg gctgatctgc
     4441 tgcatgcgga tgagcgcgtc gattgcatcg ctgtacattg cccgcgcttc gctaggatga
     4501 gcgactaact gttgtaaata agtggtattt cctaagtcgg tgaggagtaa aaatccatcg
     4561 gttaaattct gagataagat gctgggaaca ttcacatcag ccgttgagaa aagctgagca
     4621 attttgataa agggtctaca gtcttcatgt tgaggcggcg catccattgc aatgaggctg
     4681 cgatcctcaa attgaactcg gaaatagcgt cgaaaactag catcagcaga agcaatcgat
     4741 aaatcaaaat gttgattagg aaattcgttg tgaagccatg cttttaattg atgtagacga
     4801 tcagtttcca caaatacccg atattattgt ttaaaaaaat tgaaagcatt atcaacaaaa
     4861 aataaaggcc agtccatttt tttggactag ccttttatat aaacaaacag aaaagttttt
     4921 ttagtatgcg ataccgagtg ttgctaatgt ttctttagta atctgaccga ctgtcaggtt
     4981 attatctttc tggaattttt tcatcgcttc agtggttttt ccaccgcttt tgccatccac
     5041 agggcctggg ttataaccag catctgtcag cgcttgttgt aactgacgaa tgatttccgg
     5101 tgttggttta acaaccgctt taagatcctc tgttggagag tctgcgctcg gtgttgccat
     5161 ggctgtcaga gaattgacgc tttcactaac ctcagttttg atgtcttcag cgaccgcaga
     5221 aattttttct ccgacatccg ctgcaatttc ttcagttgct tgaatagttg cttcaactgg
     5281 gctgggttta gctggctcag cggctgcctt ttcaggttct tttttctcag gctcttttcc
     5341 tttgtcgcca cttgcagcgc ctgcttcttt cgctttttta tgctcggctg ccgcttcttt
     5401 ccatgccggt tcagggaggc ctgccgcatc tccaccgcca aatcgtttag caacgcgatc
     5461 agagaatgat tcttttcctt gctctgcgcc acaaccgatt aataataacg aagctgctgc
     5521 agcaattagg ctacgtttga agtgtgattc aagttgtttg gtttccatat ttaattctcc
     5581 ttccattttt attgttatta gtaattaatg actcaagtta aacccaaaat atcgaacatg
     5641 ttgaaagtta agattcatat agtttcctta ctcctatgat tgatagacga attttcccaa
     5701 ttaatgattc acttaataca acgctcggtg gttatagctc gttgtattgt tatgattaat
     5761 ttcctaacgg atgcgattaa gtacactaac gctggagact atcacagtat ttgtcaaaaa
     5821 attgtcaaga aggcggagtg ggttgtggat tagaataatc acgctgaatt ccaagcattg
     5881 tttttatcat cagtaatgct aacgggtaag ttgccctaca tttttttgga tttaccgtga
     5941 ttctgagttt atcgatgcaa tcagttattt accatttctc agcagcacca aatcctctaa
     6001 tgatgcggtt tgacatcaaa aataacatcg attagattgc aattaatcga tagcttcgca
     6061 atatttgctt tgcgaaatga gcataaaatt atgagcgata tttttatcag ttatactagc
     6121 gaggatcgtg acttagcaag acaattcccc caaaattatc atccatccgc tcactgagga
     6181 gaataaacca acgggatcac tggaatcgat catattccac cttgcaccac gcttaccttt
     6241 tttactcctt gtgtcgcgtc acatcagcat accattgaat tttctgggaa tgaccttgca
     6301 cttgcgtcgc gtattacgct gactggccca tttaaactgc ttgccgccga aaacgtaacc
     6361 tcgcttatag gacgcagctc atatttaata aaaagccgtc ggtcgattta caagtcgcat
     6421 tgggtgatag tggtcctttc gtcgtggtaa ttagtgctac gcatcgtttt accgtcacgt
     6481 tgatccttgc tggccgttca aatcatgagc cattggtgaa gccaggtagc gcaattagca
     6541 gcatcgattt ttagaggcaa ggatcggatg gcaaacccgg gtctacgttc attgcactcg
     6601 gaacgattag ctacgatggg tttgcgcaga tccccgcagt gatgttggct gcacacgacg
     6661 ttcttgcact taacgatctc caggatgtca cgattcaaaa aatttattac gatccacagt
     6721 aaaatggttt tgtgctaaga ctaatcaggc aagttgataa agtagcccag caaactggaa
     6781 aatggactaa agatcttcaa agatcttcat ctcacggtgt tcgaaaattt ctggcatgat
     6841 caaaggctgg tattgatttt tagtatcatc gtaggggtgg gttcagtcgt gttcggtgct
     6901 tataaaattt atcaggaggt cagaaaacat gtctaattac ctacagcgtg atgaaagcaa
     6961 acaaattcaa ctatccggat ggcggtgttt gttaccgtgg ttgctatcca gtttttgttt
     7021 gctgcttgca agcgttagtc accagttcgc aagtgcgcaa gaaatcgata gtttgcgcaa
     7081 aggggtggtc aaagtagctg caactttaga tggaatccaa aaaaccggaa ccgaattcat
     7141 cgtccagcag gatacgaagg caacttatat tgttacggca gcacacgtcg tcgccgacga
     7201 taaataccct aaagtagcat tttttagcga gcgacataca ttcgtcgaaa cggaagtatt
     7261 acaaaatgac caacgcaatg atacttcctt attggaagtt cacgataata cgctagtgtc
     7321 aaccgatgtt atagcccttc tgtttgcggc agaagaaaag gtgcaagata acaatgaatt
     7381 gattgcaatc ggttttacac cgggtgttga gtgggcgcaa accccagtaa ctttagcctt
     7441 acgtcaagct actgatctaa ttctcgatgg taatcttgat gaaggcaatt ctggcggccc
     7501 catcttgaaa aaaggcagta ttaatgacct ggtaaccaaa ggagataata gattgggtcg
     7561 cgcaattcca gaaaaattca tcacgacggt gttagacgga tgggacatta cgctaatgaa
     7621 gggcggggta gttacagcac aatttacgtc taaaccaact cctcacgaaa aaccaccgac
     7681 cacaacccgg ttaccgatcg aacccgacat ggtagcagtt cctgcaggta aacttattat
     7741 gggctctcct aaaaccgagc cgggtcgcta ttctaatgaa ggtccgcaac atgaagtttt
     7801 cattgcatct ttcgccatta gccgaacgga aattaccgta gggcagtttc gattgttcgt
     7861 ccaagataaa gactatcatg gcggaaagga gtttcaaaca gccgcagaag agaatgagaa
     7921 ggggtgctgg acctggaatg aggaaaagaa gcaatttgaa cgaataccac aacgtaactg
     7981 gaaaagccca gggttccacc agcacgatga acatccggcg gtatgcgtat cctgggatga
     8041 cgctcaagct tatgtacagt ggttattgaa aaaaacgggc aagcaatacc gcttacccac
     8101 tgaagcagaa tgggaatatg tggcacgtgc tggcacaacc actgtttact ggtggggaga
     8161 taacattggg aaaaataatg cagtttgcgg tgactgtggc agtcaatggg atggtcgaca
     8221 aacggcaccg gtgggttcct tcaaacccaa tgcctttgga gtttatgata ctgcgggtaa
     8281 tgtatgggaa tggacgcagg attgttggca tgacgattat acgaacgcac ctactaatgg
     8341 tacggcttgg ttagagaagg ataacggtga ttgctcgcgt cgggtggttc gcggcgggtc
     8401 atggggcttc gatccattgg atctgcgttc ggctggtcgc gactggatca ttactgatgg
     8461 ggcgggttat ggtaccggct ttcgtgtcgc cagggatttt tgaggtgtgt gctgtgtgct
     8521 tttgtccttt gcggggtcag aggcgtagcc cctgagattt ttggggagga tcttagtgct
     8581 ctgcgcccaa tctgagtgat tcaatttaac aactgtgctg tagcacaccg agcgcctggc
     8641 ccgcagcgac ataattgccg ggttgacaaa aaagctgcac aacaacgcca tctgccggag
     8701 ctggtacggg aaactccatt ttcatcgatt cgatgacgag gatgggttct tgttggttaa
     8761 tctgctggcc tgcttcgatg agcaatttcc ataccgttcc actaaccggt gcggtaatga
     8821 tttcagcccc tgccggtaga tcgatttcaa tttgggcatc ggctgcttcc aggatatctt
     8881 cattaaccgt ttcaaaagcg gcggttgctt tccagcgttg gctttcttca tcaaacgcct
     8941 tttgttgctg agttttgaac gattcaatac tttcacgatg ctgctgcagg aaggcgttgt
     9001 attgcttgag attgagcgtg ctttcttcaa tacgcaattt taagcgaccg gcgatgaaat
     9061 cagcgcggtg tttaagtagc tcagcttcac tgacgggata gaatcggatc tggtcaaaat
     9121 agcgcaatag ccaaggccgg tgtgttttga atgctgctgt ttggcgatag cgattccaca
     9181 tttgcagtgt tctacccacg agctgatagc caccggggcc ttccatgcca tagatgcaca
     9241 aataggcgcc accaataccg actgcattct caggggtcca agtgcgcgcc ggattatatt
     9301 tggtcgtgac tagccgatgt cgtgggtcaa gtggcgtggc aacgggcgca cctaaataca
     9361 catcccctag ccccatgacg agatagcttg catcgaatac gatttgctta acgtcatcga
     9421 tgtgatcgag gccattgatg cggcgaatga attcgatgtt gctcgggtac cagggtgctg
     9481 ctgggcgcac cacgcggtta tatttctcga tggcgatttg cgttgctggg tcatcccaag
     9541 aaagcggcaa atggatgatg cgtgagggaa tattcatgtc gtcaagcgcg ggtaatcttg
     9601 attcacaagc catcaaccaa gcgagcagtt catcgcgcgt tcgaatgcgc gtatcaaagt
     9661 gaatctgcag cgaccgaatg ccaggcgtca aatcaataat tccttgcgct tgggcttcaa
     9721 tgagtgtcat taacgcatgg atacgaaaac ggagattgag atcgaggata ggcggaccat
     9781 attccactag caggtattta tccccagctt ggcgatacgt cacttgcacc tgattcgctt
     9841 gtactgggat ttgctggagg atgggttgac cccaatcctc tgcgatggaa ctcgtcgcag
     9901 cgggttgtgc gtgatcgaga tgctcgatca aatgattttg ctgggtttcc agcatttgcg
     9961 cattttccaa ggaaagcggt cggaaacgga tgcgatctcc gggtctgagt tgcccgattt
    10021 tccagagctc tgctgcaatg atcgtcgctg ggcaaacgaa accccctaaa ctaggaccat
    10081 cggggcccag aataatcggc atgtcaccgg taaaatcgat agtaccgatt gcataggcgt
    10141 tatcgtgaat attggaagga tgcagcccag cttcaccacc gtcgggtctt gcccattgcg
    10201 gtttgggacc gaccagtctt actccggtgc ggctggaatg atgatggact ttccaagcgg
    10261 tcgcgaagaa ttgctcgata tcagcttgcg taaaaaaatc aggcgcacca tggggaccat
    10321 agagcacaaa aatttcccaa tcatgcgtat agcggggaat gatggcttca cgcagtttct
    10381 gtgcgacaaa aggttttgct ggtttgatcg ataacacatc gcccgattgc aatgcgcgac
    10441 cggcatgccc accaaattgc cctaacatga atgtggagcg gctaccgagg taatggggta
    10501 cttgaatacc cccttgtact gcgagataaa cgcgactgcc gacagagtca attttactaa
    10561 ttttcagcaa ggtgccagcg cgaacagcgt gcgattgcca tagcgcaagt ggttcattat
    10621 cgagcaaggc ctcgatcggt gcgccacaga gcgcaattac gctagcgcaa ttaaagcgca
    10681 gtgtcggacc gttcagcgtg atttcaagcc cggctaaatg ttcagggttg ttgaccaatc
    10741 gattggccaa ccggaaggcc aatgagtcca tcggaccaga gggcgggaca ccgacattcc
    10801 aatatccgag cctgccgggg taatcttgta ccgttgtttg cgtgcctgga tcaagtacat
    10861 cgagggtgtt ggctaaatag gtaaactgac ttagaaattg cgtactgact tgacctttct
    10921 gaaacacatc actgctgatg atttgtttga gataactgag attggtttca ataccatata
    10981 aggaagtttc attaagcgcg gttattaatt tgttgactgc tgcattccgg gttggtgcat
    11041 gcacgatcat ttttgcgatc atgggatcgt agaaagcaga tagctcggta ccattttcaa
    11101 cccaggtttc aacccggcag gtttggggaa attcaaccgt tgtcagggtg ccacaacacg
    11161 gttgaaaatt tttagccgca ttctcagcat aaattcttgc ctgaatggca acgccttggg
    11221 gtttaatcgt gaaagcggtg agtggtggca gttctcccgc tgcctgtttg accatccatt
    11281 cgacgaggtc c
//
LOCUS       DAOQKQ010000081        11276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_2700_length_11276_cov_5.910258, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000081 DAOQKQ010000000
VERSION     DAOQKQ010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 11276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11276
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2700_length_11276_cov_5.910258"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..295
                     /locus_tag="PKZ49_06760"
     CDS             101..295
                     /locus_tag="PKZ49_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HND36300.1"
                     /translation="MYICICKGVTDHAIREAVHQGAERMRDLKTCLGVTEQCGICACH
                     AKMVLDQALIQQQIGSASST"
     gene            336..800
                     /gene="bfr"
                     /locus_tag="PKZ49_06765"
     CDS             336..800
                     /gene="bfr"
                     /locus_tag="PKZ49_06765"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HND36301.1"
                     /translation="MRGNSDIITWLNRQLQHELTAINQYFLHARMYKNWGFNKLAEHE
                     YNESIEEMKHADQLIERILFLEGLPNLQELGKLLIGENAQECVSCDLKLELISRETLV
                     AGIAACETSQDYISREIFEKILEDTEEHIDWLETQLDVMQKVGMQNWLQSQI"
     gene            958..3384
                     /locus_tag="PKZ49_06770"
     CDS             958..3384
                     /locus_tag="PKZ49_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HND36302.1"
                     /translation="MVENLIKNGQFTSITRTIQKQLSKSGIVFFITITTFFSSAGIVH
                     SSALNESAVVNLNIPAASLNEALNQFSKQTGIKIAFDPEQFQGKTAKALIGEFTLKEA
                     INQLISGNKLTAVPVGSGYTLLEIADSSVSNPVVTTLPSIQITATKTASRYLATSSIT
                     ATKTNTLLRDVPQSISVITEGIIKDQAIRSLSDAVRYIPGIGVSQGEGNRDALIFRGN
                     RSTGDFFVDGIRDDVQYLRDLYNIERVEVLKGANGMIFGRGGSGGVINRVTKQAGWDP
                     IREFSFQGGSFNLKRMTMDVNQAINEHVAFRVNGLFEDAGSFRNGVESQRLGISPTVT
                     IKPTDRTKIVVNMERFHDERVADRGIPSFQGRPADVKSSQFFGDPRRSNSNIEVLSFN
                     SLIEHRFESGVMLRNRTNYAEFDKFYENVFANGPVFGNFVPLSAYNNATDRQNIFNQT
                     DLLYTLETGPITHTLLAGVEVGQQITNNRRETGFFNNDLSRGQSIRVPFGNSITNAPI
                     TFVNAEGDAFNRSKVDITSFYFQDQIELLPQLQAIAGVRYDLFEVNFQQKNGNQDRLN
                     TTDGLLSPRFGLIYKPIEPVSIYVSFSQAFVPRAGEQLTSLTVTTDTLKPEKFTNLEA
                     GFKWDIRPDLSFTGAAFRIDRTNVITIDPTNAARSFLTKGQRTEGVELSLSGQLLPNW
                     NVMGGYAYQIGEVTSDQAGAPKGNSVAELPRNTFSVWSRYDLTPKFGAAIGVIYRSSM
                     FASTSNTVIIPDFTRVDAALFAQLTTRVRAQLNVENLFDAKYFASVHNDNNITPGSPV
                     AIRASLIANF"
     gene            3732..5900
                     /locus_tag="PKZ49_06775"
     CDS             3732..5900
                     /locus_tag="PKZ49_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36303.1"
                     /translation="MSVRAASSAELIQSGVTAYQTIGTLRRETPINGDAIAAAYTGDL
                     QALVQEIDEANVLELHNDIQGAINDIKNGKAPKLAAQVVDKTLQRVFYQIIWNRISAI
                     RSEFQSGALSTLVSMLDEAEAAFNAIKGTVARENRVLTADRQSIVAGDNPGLDTEVLD
                     QFARVRVALNKTNAEEDFIVVQIARYAIRMSLARAYYIGVLREVAAVIENRNSDVEET
                     LEVLKEGEIFYRIIESQISNGNPAGNLIIKNQLVGHPSKVVADEIVSELGKGFIGRVL
                     AETSGQERAITEGNRPQAVAEAAGAKYFARVLFPDLELRLGSTVRSNLENELENLLAA
                     SNELNATNSKVARDAIAGILNQYESALNRSKYTVTKPTALIENAVVDFQEIGVLRNQS
                     PADADAIAQKYSGDLKGLTQIVDQIYGLTIDQDVSAAINRVKNGDDTALALQVIDKSL
                     QRMFAIVVYNRVVLTVDQFANLSTDELLIEWERAYSAYLAIARTADREDKILTADKTT
                     IMSGRNPDLDYQILTAFVQGKEVLSKANDDDRASIALAQENIIIPLVRSFLIGVLREV
                     EGIISDRDGNVDEAREKQVEGEYFYRVVEGFISQDNLVGSNLIKTQLTGSLASVEADA
                     IVKQINKGILGQLKRNISQIEVNFASDKSKALLARERLFLLAGIFLSDLELRLGALQR
                     VRLENAIRNLKEAILTDDSSQAIATRAVMTEVIANYESKL"
     gene            complement(6098..6754)
                     /locus_tag="PKZ49_06780"
     CDS             complement(6098..6754)
                     /locus_tag="PKZ49_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36304.1"
                     /translation="MENSSILDFTSPGSIPLESSEIVKQVNLEIRISIQTLENILVTD
                     PMETTCWKLLGGLYLGANLTNKFNKLTQQYQNFFGKPLFPEFEEKNRAEEQLLFRMPS
                     NIVPELLPNTTEVEQACNSRKKVTIDFSDVKESSAEGLSTLAAFFLSLAKVSNKPEII
                     DINHFILNLEKKAIASNKVNSVWDVLFAYKRLCDDVKGFDNLALKFAIQYSISPPSWI
                     "
     gene            complement(6775..8919)
                     /locus_tag="PKZ49_06785"
     CDS             complement(6775..8919)
                     /locus_tag="PKZ49_06785"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HND36305.1"
                     /translation="MSEANALFLEASKYLKPEELKLLSRSYYFSQHAHQGQFRKTGDP
                     YISHPVAVAKILCSLRLDALTLASALLHDVVEDTDTSKAEISENFGETVAELVDGVSK
                     LDKIESQTQADAQAENFRKMLLAMAQDVRVILIKLADRLHNMRTLGVMRTDKKKRIAR
                     ETLEIYAPIAHRLGLNNIYQELQELGFQHFYPNRYKVLTKATKAARGNRREVVSKILE
                     AIKQKLLNAGIDAIVTGREKHLYSIYKKMSEKQLSFSEVLDIYAFRVLVKDTRSCYAA
                     LGALHGLYKPIPGKFKDYIAIPKPNGYQSLHNTLLGPYGLPLEIQIRTYEMHRIAETG
                     VASHWLYKSSDNEDNDLHLKTNQWLKSLLETLSDSSDSVEFLEHLKIDIFPGDVYVFT
                     PQGKILALPRGSTVVDFAYAIHTDVGNRCVAAKVNSENTPLRYKLKNGDRVEIITAPT
                     ANPNPGWLSYVNTGRARSSIRHFLRTIQYDESAELGERLLNQVLLSFNIIPETITPVQ
                     WDKLVKDSGVKTKKALFAEIALGKQFPVVVAKRLTAPYEALSEAFSTSNNPISILGTE
                     GMTIQFAKCCYPIPGDAIAGLIKKDHGLVIHTQDCTAISKYLKNSENLLNVSWGEEIP
                     NTFQSAIIMTVVNQRGVLARVASEIAKVGSNIDDVKLENDSDFTNMRFILQVRNRKHL
                     AQIMRKLRHIHEIVKLGRVKAGIEHKYHKGSI"
     gene            complement(9002..9205)
                     /gene="rpoZ"
                     /locus_tag="PKZ49_06790"
     CDS             complement(9002..9205)
                     /gene="rpoZ"
                     /locus_tag="PKZ49_06790"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HND36306.1"
                     /translation="MARITVDDCLEIIPNRFDMTLAATVRARQISIGSTPLIENEKDK
                     PTVIALREIARGKINADILNTIR"
     gene            complement(9226..9831)
                     /gene="gmk"
                     /locus_tag="PKZ49_06795"
     CDS             complement(9226..9831)
                     /gene="gmk"
                     /locus_tag="PKZ49_06795"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HND36307.1"
                     /translation="MSSLIIISAPSGTGKTSIVSALTESDSQLSLSVSHTTRRPRPNE
                     VEGQDYYFVDRDIFQAMVNRGEFLESAEVYGNLYGTSHKWVKESLASGRDILMEIDYQ
                     GAQQVRKAFPQAISIFILPPSYAQLEERLKQRGQDDQKTISRRLATVREEVSHLNEFD
                     YVVVNNELQEAIKDVNCIIKAERLRIMRQLINQRTLVAQFY"
     gene            complement(10032..10730)
                     /locus_tag="PKZ49_06800"
     CDS             complement(10032..10730)
                     /locus_tag="PKZ49_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36308.1"
                     /translation="MQTNTDSTNSHEDDLKKLESDIREAIAHGGNVKEAVHKLTLNAM
                     QANRLDIDSLGRIASAVMQGIHEGASQKLALASEQSHIAKDQISHAVSGLDTALAQFV
                     GASKLALEEAAGKAKQFSREELSKTRADLESLESLFLETLQRTASTAKGLIADALNDL
                     LMHAKRNGTTIGEQLKDTLATFTHQLSSVSHSQIEASLQLAQTTADLLHKISSGILSG
                     ISEQTKRNDSENKQ"
     gene            complement(10776..>11276)
                     /locus_tag="PKZ49_06805"
     CDS             complement(10776..>11276)
                     /locus_tag="PKZ49_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HND36309.1"
                     /translation="PCFRSEAGSYGKDTRGLIRQHQFDKVELVHIVHPHHSFEALEQL
                     VHHAETILQKLELPYRVMVLCTGDMGFSAAKTYDIEVWLPAQNTYREISSCSNCEAFQ
                     ARRMQARFRNEKGKTELLHTLNGSGLAVGRTLVALLENYQNADGSITIPSVLKKYMNN
                     LDRITI"
BASE COUNT         3350 a         2013 c         2350 g         3563 t
ORIGIN      
        1 ttatagatag atggcaaatc agttacttgc actatttgaa atggtgcaat ataattacga
       61 atcattctca ttaacaattt ttggtttata ggtttaactc atgtatattt gtatctgtaa
      121 gggtgttacg gaccatgcga ttcgcgaagc tgtgcaccaa ggagcggagc gtatgcggga
      181 tttaaaaacg tgtttgggcg ttaccgaaca gtgcggaatt tgtgcatgcc atgctaaaat
      241 ggtattggat caggcattga tacagcagca aattggctcg gcctcttcca cctaaattga
      301 tgtgcttcac aagttaaacg ctctaggagg taataatgag aggtaattcc gatataatca
      361 cctggttaaa tcgccagctg caacatgagt taacagcaat caatcaatat tttctccatg
      421 cgcgcatgta taagaactgg ggctttaata agcttgctga gcatgaatac aatgaatcga
      481 ttgaggaaat gaagcatgca gatcaattga ttgagagaat tttattttta gaaggtcttc
      541 ccaatttaca agaattagga aaattattaa ttggtgaaaa cgcgcaagaa tgtgtttcgt
      601 gtgacttgaa actagaactt atttctcgtg aaacactagt cgctgggatc gcagcctgtg
      661 aaacaagcca agattatatt tctcgagaaa tttttgagaa aatcttggaa gatactgaag
      721 agcatattga ctggcttgaa acgcaattgg atgtgatgca aaaggttggt atgcagaact
      781 ggttacaaag tcaaatctaa ttttcttact taatgctgct ggcccttcat tctttgtcag
      841 cagcattttc ctaaagctag ctaaaaattc taactataag cttggttgtt attattaaag
      901 cactcttaaa ttgatcattc tgcgttgttc gacatgttcc aggcattaag aaattttttg
      961 gttgaaaatt taattaagaa tggtcaattt acctctataa ctcgaacgat tcaaaaacaa
     1021 ctcagcaagt cgggaattgt tttttttatt accattacta cctttttttc aagcgctggg
     1081 atcgttcatt cgtcagcgct aaatgaatca gcagttgtta acctaaatat tcctgcggct
     1141 tcattaaacg aagcacttaa tcaattttca aagcagactg gtatcaagat tgcttttgat
     1201 ccagagcaat ttcagggaaa aacagccaaa gcattgatcg gcgagttcac tttaaaagaa
     1261 gcgataaatc aattaatcag tggaaataaa ctgacagcgg tgccagttgg ttcagggtat
     1321 acgctactag aaatagccga ttcctccgta tcaaacccag tagttacaac gttaccttcg
     1381 atacagataa cggcgaccaa aacagcaagt cgttatttgg ccactagtag cattacagca
     1441 accaaaacca atacacttct tcgagatgtt ccgcaatcaa tttctgttat tacggaagga
     1501 ataataaagg atcaagctat ccggagtttg agtgatgcag tgcgatatat tccgggaatc
     1561 ggggtctcgc agggtgaggg taatcgtgat gcgctaatat ttaggggtaa ccgatcaaca
     1621 ggagattttt ttgtcgatgg tattcgtgat gatgttcagt atctccgtga tttatataat
     1681 attgagcgtg ttgaagtatt aaaaggtgct aacggaatga tattcggacg cggtggttct
     1741 ggcggggtta ttaatcgcgt taccaagcaa gctgggtggg atcctattcg tgaattttcg
     1801 ttccaaggtg gatcttttaa tttgaaaaga atgacaatgg atgttaatca ggccattaac
     1861 gaacatgtgg catttagggt aaatggttta tttgaagatg cgggtagctt tcgaaatggc
     1921 gttgagtcac agcgccttgg aatttcgccc actgtaacaa ttaaaccaac ggatcgcact
     1981 aaaattgttg ttaatatgga acggtttcat gatgagcgtg ttgctgatcg tggtatacct
     2041 tctttccagg gaagaccagc tgatgttaag agttctcaat tttttggtga tccaagaaga
     2101 agtaattcta atatcgaggt tttgtcattt aactcgctaa tcgagcatcg ttttgaatct
     2161 ggagtcatgc tgcgtaaccg aacaaactat gcagaatttg acaagtttta tgagaatgtt
     2221 tttgctaatg gcccagtttt tggaaacttt gtacccttga gtgcttataa taatgcaacc
     2281 gatcgtcaaa atatttttaa tcagacagat ttgttgtata cccttgaaac aggacctatt
     2341 actcatacgt tgttagcagg ggtcgaggtt ggtcagcaaa ttactaacaa cagaagggaa
     2401 actggttttt ttaataatga tttatcacgg ggtcaaagta ttcgagtacc atttggtaat
     2461 tcaattacca atgcgccgat cacatttgtg aatgctgaag gagatgcttt taatcgcagt
     2521 aaggtcgata tcacttcctt ttattttcaa gatcagattg agttattgcc ccagctgcaa
     2581 gccattgcag gggttcgtta cgatttgttt gaagttaatt ttcagcaaaa gaatggaaat
     2641 caagatcgct tgaatactac cgatggtttg ctatcccctc ggtttggttt gatttataaa
     2701 ccaattgaac cagtctcaat ttatgtgagc tttagtcaag cttttgttcc tcgtgctggg
     2761 gaacagttaa cttcgttaac tgttacaacg gatacgctga aacctgaaaa atttacgaat
     2821 cttgaagcgg gttttaagtg ggatatccga cctgatttat cgtttacagg tgctgcattt
     2881 agaattgaca gaacaaatgt tattacgatt gacccaacta atgctgctag gtcctttcta
     2941 acaaaaggac agcgaacaga aggggttgag ctaagtttaa gtgggcaatt gctaccgaat
     3001 tggaatgtga tgggagggta tgcataccaa attggtgaag taacgagtga tcaagctgga
     3061 gctcccaaag gaaattcagt cgccgagctc cctcgtaata cattttctgt ttggagtcga
     3121 tatgatttaa caccaaaatt cggcgcagcc atcggtgtta tctatcgtag tagtatgttt
     3181 gcatcaacat caaatacagt tattattcca gattttacac gtgttgacgc cgcattattt
     3241 gcacaattaa caacgcgggt ccgagctcaa ttgaatgttg agaatctttt tgatgctaaa
     3301 tattttgctt cagtgcataa cgacaataat attactcctg gatctccagt tgcaattcgt
     3361 gcctcattaa ttgcaaattt ctaaagccat gcttttaatc tctccaagtt ttgtttttct
     3421 ccctctagta cataagctat tgaagcaata aaatttgcta ctggtcttgt tgaattaata
     3481 gggctcgggt agagtggtac tggcggtcta gttacttctc ttggagctat atcgcagatt
     3541 tcttagagaa tctctctaaa gataatttta caatatttga ttttattaat aataatcatt
     3601 atcattaacc ttcgtattca atattaatta tcaaggggga ttttgtaatg cttttaagaa
     3661 agcagttatt caatttaata gtttcttcat cattgataag tttattgtca ttgggtttag
     3721 cgttaagtag tgtgagtgta agagcagctt caagtgctga actaattcaa tcgggcgtga
     3781 cagcttatca aacgattggg acgttacgca gagagacacc aattaatggc gatgccattg
     3841 ctgcagctta tacaggtgat ttgcaagcgc tggtacaaga aattgatgaa gcgaatgttt
     3901 tagagctaca taatgatatt caaggtgcaa ttaacgatat taaaaatggg aaagcgccaa
     3961 agttggctgc acaggttgta gataaaacct tgcagcgtgt tttttatcaa attatttgga
     4021 atcgaatttc agcaatacgt agcgaatttc agtcaggcgc attatctacc ttagttagta
     4081 tgttggatga ggcagaagca gcatttaatg caatcaaggg aactgtagcc agagagaatc
     4141 gagtgctaac tgctgataga caatcaatag tagcgggaga taatcctgga ttagatactg
     4201 aggttctcga tcaatttgca cgagtaaggg tagcgctgaa taaaaccaat gctgaagaag
     4261 atttcattgt ggtgcaaatt gctcgttatg cgattcgtat gtctctggct cgagcatact
     4321 atatcggggt tctacgtgaa gtggctgcag tgatcgaaaa tagaaattcc gatgtagaag
     4381 agacccttga agtactaaaa gaaggagaaa tcttttatcg catcatagag tctcagatat
     4441 caaatggaaa tccagcaggt aatttaatca tcaagaacca attggtaggt catccatcaa
     4501 aagtggttgc ggatgagatt gtgagtgaat taggtaaagg atttattggt cgagtgcttg
     4561 cagaaacaag cgggcaagaa agagccatta ctgaaggcaa tcgtccacag gccgtagcag
     4621 aagcagctgg cgctaaatat tttgctagag tcttatttcc agatttggaa ttgaggttgg
     4681 gatcgacagt gcgaagtaat ttagaaaatg aactcgaaaa tttgctagca gcgagtaacg
     4741 agctaaatgc aacaaactca aaagtagcac gcgatgctat tgcagggatt ctgaatcagt
     4801 atgagagtgc gcttaaccgt tcgaaatata cagtaacaaa acccactgcg ctcatagaaa
     4861 acgcagtagt ggattttcag gaaattgggg ttttaagaaa ccaaagccca gcagatgccg
     4921 atgccattgc gcaaaaatat tctggtgatt taaaaggttt gacacaaatc gttgatcaaa
     4981 tttatggatt aacgatcgat caggatgttt cagcagcgat caatcgagta aaaaatggcg
     5041 atgatactgc tctagcattg caagtaattg ataaatcact acagagaatg tttgcgatcg
     5101 tggtttacaa tcgtgtagtg ttgacagttg atcaatttgc caatctttcg accgatgagc
     5161 tactcatcga atgggaaaga gcttatagcg cttatttggc tattgctaga acggcagatc
     5221 gggaagataa aatactgaca gcggataaga caacaatcat gagtggcagg aacccagatc
     5281 tagattatca aattcttact gcgtttgttc aaggtaaaga agtcttaagc aaagctaatg
     5341 acgatgatag ggcctcaatt gctttggcac aagaaaatat cattattccc ttagtaagaa
     5401 gcttcttgat cggtgtgcta agagaagtag aaggaattat ttctgatcgt gatggtaatg
     5461 ttgatgaagc tagagaaaaa caagtcgaag gggaatattt ttaccgcgtt gtggaaggat
     5521 ttatttcaca agataactta gttggcagca atctcatcaa gacacaacta actggtagtt
     5581 tggcctctgt tgaagctgat gcaatcgtta aacaaataaa taaaggaatt ctgggccaac
     5641 ttaaaagaaa tatcagtcaa atcgaggtta attttgcaag tgataagagt aaggctttgc
     5701 tagccagaga gaggcttttt ttacttgcag ggatatttct atcggatcta gagttacgct
     5761 taggtgcatt acaacgagtg aggttagaaa atgcaattcg gaatcttaaa gaagccatcc
     5821 ttactgacga ttcgtctcaa gcaattgcta ctcgtgcagt gatgaccgaa gttattgcaa
     5881 attatgagag taaattgtag ctttctcata aatattagaa tgctgggtaa ctggatcaat
     5941 caataaatat caaaacaaag ccactgtcag ctacatgaga gcttcgatag tggcttaaca
     6001 gaaatttagt tggcgttttg agaaaaacat aaatctactg atattgatgg caagttcagc
     6061 agaaatattt tatcgtgata actaaggatt gttgaagcta aatccaagaa ggtggagaaa
     6121 tactatactg aattgcaaat ttaagagcta aattatcaaa cccttttaca tcatcacaca
     6181 gacgtttata agcgaaaagt acatcccata cgctatttac tttattgctg gctatcgctt
     6241 ttttctctag atttaatatg aaatgattta tgtcgattat ttcgggttta ttactaactt
     6301 tggctagtga tagaaagaaa gctgctagtg tacttaatcc ttctgcactt gattctttaa
     6361 catcagaaaa atcaattgtt acttttttgc gagagttaca ggcctgttct acttctgtag
     6421 tgtttggtaa taattctgga acaatattag aaggcattct aaacaacaat tgttcttcgg
     6481 ctcggttttt ttcttcaaat tcaggaaata atggttttcc aaagaaattt tggtattgtt
     6541 gtgtaagctt gttgaattta ttagttaggt ttgctcctag ataaagccct ccaagtaatt
     6601 tccagcacgt tgtttccata ggatcagtga ctagaatatt ttcgagtgtt tgaattgaaa
     6661 tcctaatttc cagattaact tgcttaacta tctctgagga ctctaaagga atcgagccag
     6721 gggatgtaaa atcaagaata gaagaatttt ccattgtgat aacttaagtt aaagttaaat
     6781 actaccttta tgatatttgt gctcaatacc agctttcact cgtccaagtt ttacaatttc
     6841 atgaatatgc cttagctttc tcatgatttg agcgagatgc ttcctatttc tgacttgtaa
     6901 aataaaacgc atattagtga aatcactatc attttctagt ttgacatcgt caatattaga
     6961 cccaacttta gcaatctcag aagcgactct tgcaagaaca ccgcgttgat ttaccactgt
     7021 cataataata gctgattgga atgtatttgg aatttcctcg ccccaactca cattcaaaag
     7081 gttttctgaa ttctttagat attttgaaat tgctgtgcaa tcttgggtat gaatgactag
     7141 tccatgatct ttttttatca atccagcaat ggcatcacca ggaattggat aacagcactt
     7201 ggcaaattga atggtcatgc cttcagttcc tagtatcgat ataggattat tgctagtact
     7261 aaaggcttcg gacagcgctt cataaggagc agttaatcgt tttgcaacaa caacaggaaa
     7321 ttgctttcct agtgcaattt cagcaaaaag tgcttttttg gtttttacac cactatcttt
     7381 tacaagctta tcccattgaa ctggagtgat agtttctgga atgatgttaa acgatagtaa
     7441 tacttgattt aagagacgct cgcccaattc tgctgattca tcatactgaa tggttctgag
     7501 gaaatggcga atacttgacc gtgctcttcc agtattaaca taggataacc acccaggatt
     7561 tggatttgcc gttggtgcgg ttatgatttc aactctatca ccatttttca gtttatagcg
     7621 gagtggggta ttttcgctat ttacttttgc ggccacgcag cgattcccaa catcagtatg
     7681 gattgcatat gcgaagtcaa ctacagtaga gcctcgtggc aatgccagta tcttaccttg
     7741 aggtgtaaaa acatatacat cacctggaaa aatgtctatt ttcaagtgtt caagaaattc
     7801 aactgaatca gaggaatcac tgagtgtttc tagtaagctt tttaaccatt gattggtttt
     7861 taaatgtaag tcattatctt cattatcgct acttttatat aaccaatgag atgcaacacc
     7921 ggtttctgct attcgatgca tctcataggt acgtatctgg atttctagtg gtaacccgta
     7981 tggacctagc agagtattat gaagtgactg atatccattc ggttttggaa tggcaatata
     8041 atctttaaat tttcctggta ttggcttata taaaccatgt aaagccccta aagctgcgta
     8101 acatgatcga gtgtctttga ctaatactcg aaaagcgtag atatctagaa cttctgagaa
     8161 gcttagctgt ttctcagaca tcttcttgta aatactataa agatgtttct cccgaccagt
     8221 cacgattgca tcgataccag cattgaggag tttctgctta atagcttcta gtattttact
     8281 aactacctcg cgacggtttc cacgtgcagc cttggttgcc ttagttaata ctttatatcg
     8341 gttaggataa aaatgttgaa acccaagttc ctgtaattcc tggtagatat tatttaatcc
     8401 gagtcgatgt gcaataggtg cataaatctc aagtgtttct cgagcaatac gttttttttt
     8461 atcggtccgc attacaccga gggtccgcat attatgcaag cgatcagcaa gcttgattaa
     8521 aatgacgcgt acgtcttgtg ccatagcaag cagcatcttg cgaaaatttt ctgcttgagc
     8581 atcggcctga gtttgtgatt cgattttatc gagcttagaa accccatcaa caagctctgc
     8641 aactgtttcc ccgaaattct cgctaatctc tgcttttgaa gtatcggtgt cttcaacgac
     8701 atcgtgtagt agcgcagaag ctagtgtcaa tgcatcaagt cgaagactac ataaaatttt
     8761 agctactgcg acaggatggg agatataagg atcccccgtt ttacggaatt gaccttgatg
     8821 agcatgttga ctgaaataat aagagcgact gagtagtttt agctcttcag gcttaaggta
     8881 tttagatgct tctaaaaata acgcattagc ctcagacatt tccataggaa cttaagcgct
     8941 acgtttgacg tgctgcaacg aagtgtattt ggaaatgggt agtagaagtt atttcaaatt
     9001 actagcgaat agtattgagt atatcagcat tgattttacc ccttgctatt tcgcgcaagg
     9061 caataacggt gggtttatcc ttctcgtttt caatcaacgg agttgatccg atcgaaattt
     9121 gacgggcccg cacagttgca gccagtgtca tatcaaagcg attaggaatt atttctaaac
     9181 aatcatcaac ggtaatacgt gccatcagtt tctctcttca aaaaattaat aaaattgggc
     9241 gactagcgta cgctgattaa taagttgtcg cattatcctt aatcgttctg ctttaatgat
     9301 acagttaaca tccttaatgg cttcttgtaa ttcgttatta acaacgacat agtcgaattc
     9361 attaaggtga ctcacttctt cacgaacggt agcaagacgt cgtgaaattg ttttttgatc
     9421 atcttgcccc ctttgtttta gacgttcttc aagctgcgca taggatgggg gtaaaataaa
     9481 aatactaatt gcttgaggaa atgctttgcg tacttgttgc gctccctgat aatcaatctc
     9541 catgagtata tctcgtcctg aagcgaggga ttccttaacc cacttatgag aagttccata
     9601 caaatttcca tatacttcag cactttcaag aaactctcca cgattcacca tcgcttggaa
     9661 aatatctcga tcaacaaaat agtaatcctg tccttccact tcattaggtc ttggtctgcg
     9721 agtagtatgc gatacagata aacttaactg actatcagat tcggttagcg cgctgacgat
     9781 actagttttt cctgtcccag atggggcgct aataataata aggctactca tgttggtgat
     9841 tatattggat attgcggtat tctggataat ataatgctaa ttataatgtc tctatttgat
     9901 ttgatctggg ttttatcggt aagaaatata tcttttataa aagagagaag ctaacttagc
     9961 gattcgaata accttttaag cagatttttc agtaataaat tcgattctat atatcttttt
    10021 caaattaggt actattgctt attttctgaa tcgtttcgtt tcgtttgctc cgaaattcca
    10081 gacaaaattc cacttgaaat tttatgaagt agatctgctg ttgtttgggc gagttgtaaa
    10141 ctagcttcaa tttgagagtg acttactgaa gacaactgat gtgtgaacgt tgccaaggta
    10201 tctttgagtt gctcaccgat cgttgttcca tttctttttg catgcataag gagatcatta
    10261 agggcatcag cgatcagtcc ttttgctgtc gacgcagttc gttgcagagt ctcgagaaat
    10321 aagctttcta gactttctaa atcagcacgt gttttagaga gttcctctcg tgaaaattgc
    10381 ttggcttttc cagctgcttc ttccaatgct aacttggaag caccgacaaa ttgggccaaa
    10441 gcagtgtcaa ggcctgagac tgcatgacta atttggtctt ttgcaatatg tgactgttca
    10501 gaagcaagtg ctaatttttg gctggcacct tcatggatac cttgcataac ggcactagca
    10561 attcgtccga gtgaatctat atcaagtcgg ttagcttgca tcgcgttgag tgtaagttta
    10621 tggaccgctt cttttacatt tccaccatgg gcgatagcct ctcgaatatc tgactcaagt
    10681 ttttttaaat catcttcatg ggaattggtg ctatcagtat tggtttgcat tttgtttctc
    10741 ctgaattaaa ttaaaaatta ttggatgtac ccgtgttaga ttgtgattcg atcaaggtta
    10801 ttcatatatt tctttaaaac agatggaatg gtaatactac catcagcatt ttggtaattt
    10861 tccaaaagag cgacaagcgt tcgaccgact gctagacctg atccatttaa cgtatgtagt
    10921 aattctgttt ttcctttttc attgcggaat ctagcctgca tgcgtcgagc ttggaaagct
    10981 tcacaattac tgcaagaaga aatttcacga taagtatttt gtgcaggtag ccatacctcg
    11041 atatcatagg tttttgctgc tgagaatccc atatctcccg tacagagcac catgacgcga
    11101 taaggtaatt caagtttttg caaaatggtt tcagcatgat ggaccaactg ctctagcgcc
    11161 tcaaaggaat ggtgtggatg gacaatgtgg actagctcca ccttatcgaa ctgatgttgg
    11221 cgaattaacc ctcgtgtatc tttaccataa ctccctgctt ctgagcggaa gcaggg
//
LOCUS       DAOQKQ010000082        11197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_2726_length_11197_cov_6.333603, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000082 DAOQKQ010000000
VERSION     DAOQKQ010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 11197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11197
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2726_length_11197_cov_6.333603"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /gene="tgt"
                     /locus_tag="PKZ49_06810"
     CDS             <1..952
                     /gene="tgt"
                     /locus_tag="PKZ49_06810"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HND36310.1"
                     /translation="LQELQAQIILGNTFHLWLRPGLGVIEAHGGLHQFMQWHGPILTD
                     SGGFQVFSLGQLRKIQEQGVKFRSPINGDLCFLSPEESMRIQRILNSDIVMIFDECTP
                     YLVDFAIVKASMELSLRWAERSKRAHEGNSNALFGIVQGGMHENLREQSLQGLVQIGF
                     DGYAIGGLSVGEPKEDMQRILKHTAPLLPEDKPRYLMGVGTPADIVHAVAQGIDMFDC
                     VLPTRNARNGWLYTRNGIIKLRNSGYRLATQSPDEKCQCYTCKNFSLSYLHHLQKTNE
                     ALGARLNTLHNLYYYQQLMREIRHAIETHQFEQYQREFMG"
     gene            1014..1478
                     /gene="yajC"
                     /locus_tag="PKZ49_06815"
     CDS             1014..1478
                     /gene="yajC"
                     /locus_tag="PKZ49_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HND36311.1"
                     /translation="MLISEAFAQAASGSQTEPGLMSLLPLIGILVIFYLLLIRPQSKR
                     AKEQIKMREALQKGDEVIISGGELGRVVSVGDNYVTLEIAPNVEIIVVKSGVQTLLPK
                     GTLKSIDTGKSSKSTKNNKTQKVTDTTESSNDDKNGEATDKETPSEGNKKES"
     gene            1539..3446
                     /gene="secD"
                     /locus_tag="PKZ49_06820"
     CDS             1539..3446
                     /gene="secD"
                     /locus_tag="PKZ49_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HND36312.1"
                     /translation="MNRYPLWKNLIIVISLFFGLLYTLPNFFGESPAIQISPLRIATA
                     TVNPELIKKVEDSLRKANIPTNGTIVEGSSIKVRFAETDTQIKAKDLLQAELGNDYIV
                     ALNLIPNSPDWLSSVGALPMYLGLDLRGGVHFMLQVDMVGALTKALDRYSADIRSSLR
                     EQKVSFSGIERERGQLTIKFRDEPARAKGETELKRIYPDLELRTTNMDRNFLLIATIK
                     PDAHARMQTAALQQNITTLRNRVNELGVAEPIIQQAGMDRVIVQLPGVQDTAKAKDIL
                     GRTATLEVRLVDDESDIDAALRGQAPLGTELFTERGGSPLLVKKQVLLTGDRITDAQP
                     GFDSDNQPAVHVNLDSNGARIFKDLTRNNVGKRMAILLVEKNLSEVITAPVIREEIGG
                     GRVQISGRMSTIEARDVALLLRAGALAAPMEIIEERTVGPSLGAENIARGFNSNLFGF
                     LAVAIFIMIYYRAFGVISVIALGANLLLLIALLSILQATLTLPGMAAIALTVGMAIDA
                     NVLINERIRDELRSGATPHMAISAGYERAFGTILDSNITTLIAGLALFSFGSGPIKGF
                     AVVLCLGIMTSIFSAVLVSRSLVNFLYGNQRKLENISIGTVWKSAVKTKRSGKNVKTK
                     NSELTNPIKDN"
     gene            3450..4382
                     /gene="secF"
                     /locus_tag="PKZ49_06825"
     CDS             3450..4382
                     /gene="secF"
                     /locus_tag="PKZ49_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HND36313.1"
                     /translation="MEFFRFERDIPFMSWGKYTTTISLLTFVLSVFFIAYKGLNLGVD
                     FTGGTVLEVNYAQPADLNKIRGTFSSIGISDVSVQNFGSSRDVMIRLPIKGDLSAAKL
                     SEIVIDALKKADDTVEVRRVEFVGPQVGQELLENGALALLFVSLGIVAYLAMRFEWKF
                     GVAGIIANLHDVIIILGFFAFFQWEFSLTVLAAVLAILGYSVNESVVIFDRIRENFRK
                     MRRAEVNQVINNAITRTMSRTIITHGSTQIVVLAMLFFGGEALFYFALALTIGILFGI
                     YSSVLVASPILLLLGVKREDLVTPEVKPQEEAVP"
     gene            4429..5034
                     /locus_tag="PKZ49_06830"
     CDS             4429..5034
                     /locus_tag="PKZ49_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HND36314.1"
                     /translation="MSEIINWIVETVGMLGYAGIVIMMFLESSFVPFPSEVVMIPAGY
                     LAFKGEMNLMIAILCGIVGSLSGALFNYYLAIKLGRPLLIRYGTYVMFNEDTLSRMEN
                     FFNRHGHISTFIGRLIPVIRQYISLPAGLANMNLVIFSLYTSLGAGIWVIILALLGYY
                     IGENEVLIKAYLHNIIVVLLLFCIIGATLYYWRYQRKQKRF"
     gene            complement(5042..5506)
                     /locus_tag="PKZ49_06835"
     CDS             complement(5042..5506)
                     /locus_tag="PKZ49_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36315.1"
                     /translation="MQASSDFQMNLNHFSVEIATESLRNLNLLQSSESTPPSLLNRMT
                     AQMQADAVFAGKLILAIQNLAVSEELDTNHQLIDKLNEAQHYINQFCDELGLRYKPGN
                     EHSASDNNNDDHSYSDAVSAADNLHSIIEILCSVIKTPSQSAEGIASKFFVV"
     gene            5705..6712
                     /locus_tag="PKZ49_06840"
     CDS             5705..6712
                     /locus_tag="PKZ49_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HND36316.1"
                     /translation="MDLLTQGLLGAALAQAGSKQQETRIATGVGFFAGLLADADILIQ
                     SDQDPLLTVEFHRHFTHSIFFVPFGALIAALMLWFFLRHKLSFLRLYFYCFLGYSLSG
                     VLDGLTSYGTHLFWPISQERVALNLISVIDPVFTLILLAGVIIAFRKKTKTFARISLF
                     LAGLYLCVGWVQLQRAENMTAFLAQTRGHVIETSIVKPTLANLILWRSIYEMNGKFYV
                     DAIRVGFFSEPKVYEGETIEKFIPGIHAPKLPEKSVLADDIERFTHFSAGYIGIQPER
                     PHLLIDVRYSNLPTSTDPLWAIVIDLHRPDQHAQYQLFRDASHEIRQKFLAFLLGEHI
                     N"
     gene            complement(6805..8556)
                     /locus_tag="PKZ49_06845"
     CDS             complement(6805..8556)
                     /locus_tag="PKZ49_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HND36317.1"
                     /translation="MHSSPPEHANSSESLEKGNRQKWNAETSARGRILVMPRAGLPAK
                     ALAHILKEHNGKARKIGQSDLYIVDIPEYSEESVITALNQHPYLKYAEPDYIFYSSLT
                     PNDPYFGNAWHLSKTGATAAWDLSQGAGITIAILDTGVDSAHPDLAGKIVPGWNFYDN
                     NSNTVDVNGHGTAVAGAAAASSNNGVGVTAMAGQSKIMPVRIASPEATAYGSTIAQGL
                     TWAADQGARVANISYANVPASSTIINAAQYMKNKGGLVIVAAGNGGKDEGFTPTTSMI
                     PVSATNATDTKTSWSSFGNYVALSAPGIDIWTTYRNALYGTAWGTSLASPVVAGTVAL
                     MMAANPKLGSTEIESLLFSTAKDLGSAGRDPYYGYGRVNAAAAVQAAKSNITATDTTP
                     PIVSTIEPLSGETVSGIVPVDIQAYDNVGVTRAELWVNNTSVAVDTSEPFAFAWDSAG
                     ASNGETNLVVRAYDAAGNMSLSNAISVTVSNPIQPPIVDTEAPIISIINPLTGNVSGA
                     VTITANASDNNGASGITLSIYIDGVLKTTGIGSALSMSWNTRSKYVKAGMHTIKAIAK
                     DAAGNSSAKSVSVNVIK"
     regulatory      complement(8841..8927)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(9105..10160)
                     /locus_tag="PKZ49_06850"
     CDS             complement(9105..10160)
                     /locus_tag="PKZ49_06850"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HND36318.1"
                     /translation="MRQRVRNLSATLFLILILGTFYGTVPYYQNYFSSSTDFLGQEYS
                     HWQVLLTAMLAYVVILSIFYLTEKSPRISKSIYCLRAIQRLMRSPKSILQTGLPFEER
                     LGLLSILLKGFFIPLMVVWLFAHTGHMIEHGNKLAVALNQATIEWISLFNAHGFWFMF
                     KVILFLDVLFFTVGYMIELPSLKNEIRSVDPTLMGWFVALASYPPFNGLTSSVFAGYT
                     TDFPQFNDPILHIALNIFLLILMAFYTWASIALNLKASNLTHRGIIMHGPYRYIRHPA
                     YTCKNLAWWIGVLPAVIAATETSITATLLAVGSMLGWSIIYILRALTEEDHLRSVDNE
                     YDLYCQKVKYRFFPGII"
     gene            complement(10328..11122)
                     /locus_tag="PKZ49_06855"
     CDS             complement(10328..11122)
                     /locus_tag="PKZ49_06855"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HND36319.1"
                     /translation="MNKSFKNKVAGVASAVAIAMSASAAYAYVETQNFWGTTSQSGGI
                     VTFSNEGVGANQLKITFDNTSNFSGIITGIVFNITQNINNASVLSFVDGNGTNLASLW
                     NIGFNVNNNITPGNTVFDIAFTTNNGIIGGIYNSGVATSTANTYPDIATLVLQISDPN
                     PWNFSDVGDDSILRTQRTGANGNGSDKIVTSSSSTSTSGGTSTSSTSGGTSTSSTSGG
                     TTTSSTSGGTSNGNTVSEPGVLALLGIGLLGQALMFVARRRRLGSK"
BASE COUNT         3186 a         2118 c         2464 g         3429 t
ORIGIN      
        1 acttcaggaa ctccaagcgc aaattatatt gggaaatacc ttccatttat ggttacgtcc
       61 tggtctcgga gtcattgaag cgcatggtgg actacatcaa tttatgcaat ggcatggccc
      121 gatattgaca gattcgggtg ggtttcaagt ttttagtctg ggtcagctac gcaaaattca
      181 ggaacagggc gtcaagtttc ggtctcccat taacggcgat ctttgttttc tttctccaga
      241 agaatcgatg cgaattcagc gcatactgaa ttctgatatt gtgatgattt ttgacgaatg
      301 cacgccttat ttggttgatt ttgcaatcgt aaaggcttcg atggaattga gtttgagatg
      361 ggcggaacgt tctaagcgtg cgcatgaagg aaactccaac gcactatttg gaatcgttca
      421 aggcggtatg catgagaatc ttcgtgagca gtccctgcaa ggattggtgc aaattggctt
      481 tgatggctat gccattggtg gtctgtcagt gggcgaaccc aaagaagata tgcagcgcat
      541 tttgaaacac actgcccctt tattaccgga agataagccg cgttacttga tgggggtggg
      601 tacgccagca gatatcgtgc atgctgtagc gcaaggcatt gatatgtttg attgcgtgct
      661 gccaacacgc aatgcacgta acgggtggct ttatacaaga aacggtatta tcaagctgcg
      721 taatagcggt tatcgccttg caacgcaatc accagacgag aagtgccaat gttatacctg
      781 taaaaacttc tcattgtctt atttgcatca tttacaaaaa accaatgaag cactaggggc
      841 acggctcaat acactacata atctatatta ctatcaacag cttatgcgtg agatccgaca
      901 cgcaatcgag acccatcaat ttgagcaata tcagcgagaa tttatgggct agcctaaggc
      961 cgtgctaaaa tgcttagatt ttaaatttaa acaattatag aggagatacg cgaatgttga
     1021 ttagtgaagc atttgctcag gcggcgagtg gaagtcagac tgagcctgga ttaatgagtt
     1081 tgttgccttt aatcggtatt ttagtcattt tctacttact tttaattcgc cctcaatcta
     1141 aacgtgcaaa agagcaaatt aagatgagag aagccttgca gaagggtgac gaagtcatca
     1201 tcagcggagg tgagttgggg cgcgtcgtga gtgttggtga taattatgtc acactcgaaa
     1261 ttgcgcctaa tgtggaaatt atcgtcgtta aatccggtgt gcaaactttg ttgccaaaag
     1321 gaacactgaa aagcattgat accggtaaaa gtagtaaatc aactaaaaat aataaaacac
     1381 agaaagttac tgacacaact gaaagtagca atgatgataa gaatggcgaa gcaactgata
     1441 aagaaacgcc ttcagagggt aataaaaaag aaagttaagc attttttata cgcgttctgt
     1501 tcaaatcaaa ttctgtgtac caaaactaat taattaatat gaatcgatat ccgctctgga
     1561 aaaatctgat tattgtcatt tccctcttct ttgggttgct gtatacctta cctaattttt
     1621 ttggggaatc tccggcaatt caaatttcgc cgcttcgcat tgcaaccgca accgttaacc
     1681 ctgaattgat caaaaaagtc gaagatagct tacgtaaagc aaatattcca acgaatggaa
     1741 cgattgtgga gggaagtagc atcaaagttc gctttgctga aacggataca caaatcaaag
     1801 ccaaggatct tttacaagcg gaattgggaa acgactatat cgttgcattg aatttgattc
     1861 caaattcacc tgattggctg agtagtgtcg gtgctttacc gatgtatctt ggactcgatc
     1921 tccgtggagg ggtccacttc atgttgcaag tggatatggt aggcgcgtta actaaagcgc
     1981 ttgatcgata cagcgcggat attcgtagta gtttacggga gcagaaagtt tccttctcag
     2041 gaatcgagcg tgaaagagga caacttacaa tcaagtttcg cgatgagcca gcacgtgcca
     2101 aaggtgaaac tgagcttaaa agaatctatc ctgatttgga attgcgaacg accaatatgg
     2161 atcgaaactt tttactgatt gcaaccatta aacctgatgc acatgcgcga atgcaaaccg
     2221 cagctttaca gcagaatatt acgacattgc gtaaccgcgt taatgagctt ggtgtggctg
     2281 agcctattat tcagcaagca ggaatggacc gtgtcatcgt tcaattgcct ggcgtacaag
     2341 ataccgccaa ggcgaaagat attttaggaa gaacagcgac gctcgaagtc cggttagtgg
     2401 atgacgaaag tgatatcgat gcagcattac gcggacaggc tcctttgggt accgagcttt
     2461 ttactgagcg aggcggcagt cctctgctgg ttaaaaaaca agtattgctg acaggagatc
     2521 gcattaccga tgcgcagcct ggatttgata gcgataatca acctgctgtt catgtaaatc
     2581 tcgatagtaa tggggccaga atttttaagg atctgactcg taataacgta ggtaagcgca
     2641 tggctatttt gctggttgaa aaaaatctga gtgaggtcat tactgcgccg gtgattcgtg
     2701 aagagattgg gggagggcgg gtgcaaatca gtggccgaat gagtacgatt gaagcccgtg
     2761 atgtggcctt actgctgaga gccggcgcgc ttgccgcacc gatggaaatc atcgaggaac
     2821 ggactgtagg gcctagcttg ggtgcagaaa atatcgcaag ggggttcaat tcaaacttgt
     2881 ttggattcct agccgttgca attttcatta tgatttacta tcgagcattc ggtgtcattt
     2941 cagtcattgc gctcggtgct aacttattgc tattaatagc attactttca attttacaag
     3001 caaccctgac attgccgggt atggcagcca ttgcgttaac agtcggtatg gccattgatg
     3061 caaacgttct gattaatgaa cgtattcgag atgaattgcg aagtggcgct actccccata
     3121 tggctattag tgccggttat gagcgagcct ttggaacaat tttggactct aatatcacta
     3181 cactgattgc gggacttgcg ttattttcgt ttggatcggg tcctattaaa ggatttgccg
     3241 tggtgttgtg cttaggaata atgacttcaa tttttagcgc ggtgttggtt tcacgttctt
     3301 tagttaattt tttgtatggt aatcagcgca aacttgaaaa tatttcgatc ggaacagttt
     3361 ggaaatctgc ggtcaagact aaacgttctg gcaagaatgt aaagactaag aatagtgaat
     3421 taacaaatcc tattaaggat aattaaccta tggaattttt tagatttgaa cgcgatattc
     3481 ccttcatgag ctggggaaaa tatactacga ccatttcttt gttgacgttt gttttatccg
     3541 ttttttttat tgcttataaa ggacttaatt taggtgttga ttttaccggt ggtacggttc
     3601 tggaagtaaa ttacgcacaa ccggcagatc tgaataaaat tcgtggcacc tttagttcga
     3661 tcggtatctc ggatgtgtcg gtccagaatt ttggatcttc acgcgatgtc atgattcggt
     3721 taccgattaa aggtgaccta tccgctgcaa aattgtcaga aattgttatt gatgcgctga
     3781 agaaagctga tgataccgtt gaagttcgcc gtgtagagtt tgtgggaccc caagttgggc
     3841 aagaattact ggaaaacggt gcgctagcac ttctttttgt atcgttgggt attgttgcgt
     3901 atttagcgat gcgctttgaa tggaagtttg gagtagcagg aattattgct aaccttcatg
     3961 atgtgattat tattctgggt ttttttgctt tttttcaatg ggaattttct ttaacggttc
     4021 tcgcagccgt gttggcaatt cttggttatt ctgtcaatga atcagtcgta atttttgacc
     4081 ggattcgtga gaatttccgg aaaatgcgaa gagctgaggt caatcaagtg atcaataatg
     4141 ctattacgcg aacgatgtcc cgtaccatta ttacgcatgg aagtacacag attgtcgtat
     4201 tagctatgtt gttttttggt ggcgaggctt tgttctattt tgcactggct ttaacgattg
     4261 gtatcttatt tggtatttac tcctcagtct tagtcgcaag tcctatcttg ttattgttag
     4321 gggttaaacg tgaggatttg gtgacacccg aagtgaaacc acaggaagaa gctgttccct
     4381 aaggtatttt ctaattacct ttcgtttaat tgaaagagag tgcacagctt gtccgaaata
     4441 atcaattgga ttgttgaaac cgttggtatg ctcggttatg caggaattgt gattatgatg
     4501 ttcctggaat caagctttgt cccttttccc agtgaagtgg tgatgattcc agctggttat
     4561 ctagccttca aaggagagat gaacctaatg attgcaatct tatgtggaat cgttggtagt
     4621 cttagcggcg ctttatttaa ttattacttg gcgatcaaac taggtcgccc actcttgatt
     4681 cgatatggta cttatgtcat gtttaacgaa gatactttgt cgaggatgga aaatttcttt
     4741 aatcgccatg gccatatctc gaccttcatt gggcgtctta tccctgtcat tcggcaatat
     4801 atttctttgc cagcagggtt agctaatatg aatttagtga tctttagcct ttacacaagt
     4861 ttaggggctg gaatctgggt catcatttta gcgttgttag gctactatat tggggagaac
     4921 gaggtattaa tcaaagcata cctacataat attattgtag tgcttttact gttttgtata
     4981 attggtgcta cgctttatta ttggcgctat cagagaaagc agaagcgttt ctagtattcg
     5041 attaaacaac aaaaaactta gaagcaatac cttctgcaga ttgggatggt gttttgatta
     5101 cagaacatag tatctcaata atgctatgca aattatctgc tgctgaaact gcatcggaat
     5161 aactatgatc atcgttatta ttgtctgagg ctgagtgttc atttcctggt ttatatctca
     5221 gacctaattc gtcacaaaat tggttgatat agtgttgcgc ttcgttaagt ttatcaataa
     5281 gttgatggtt ggtatcgagt tcttctgata cagccaaatt ctgtatagct aggattaatt
     5341 ttccagcaaa tacagcatct gcttgcatct gggcagtcat tcgattaagg agtgaagggg
     5401 gggtagattc agatgattgc aatagattaa gattgcgtaa gctttctgtc gcaatttcta
     5461 cgctaaaatg attcagattc atttgaaagt cactggatgc ttgcattttg gattccctta
     5521 acaaggtata tcaaattaat atcgctaaat catgtcctat ctttaggaat gatcagcgat
     5581 aacaatcctt ctatttcatt aggattgcat tacaatttga gacaatacct aattatatga
     5641 tttttaatga ttagaacaaa taaattattc agcatcgcgt gaataagcat aataatatag
     5701 tgatatggat ttactgacac agggtttatt aggtgcggct ttagcgcagg caggttcaaa
     5761 gcagcaagaa acgcgaattg caacgggagt tggttttttt gcgggcttat tagcagatgc
     5821 tgatattttg attcaatctg atcaagaccc tttgctaacc gtagagtttc atcggcattt
     5881 cactcattcc attttttttg ttccattcgg tgcgttaatc gctgcactga tgctttggtt
     5941 ttttttgcga cataagctat cgttcttgcg actgtatttt tactgttttc taggttatag
     6001 cttaagcggt gtattagatg gcttaacaag ctatggcacg catttgttct ggccaatcag
     6061 tcaagaacgt gtcgcgttaa atctcatttc agtgatagac cctgttttta cgctgatttt
     6121 actggcaggt gtcattatcg cttttagaaa aaaaacaaaa acattcgcac gcataagttt
     6181 attccttgct ggattatatc tttgtgtggg ttgggttcag ctacaacgcg cagaaaatat
     6241 gactgctttc ctcgcacaaa cacgaggtca tgttatcgaa acgagcatcg tgaagcctac
     6301 acttgcaaat ttaatactat ggcgatcaat ttatgaaatg aatgggaagt tttatgttga
     6361 tgcgatacgc gttggttttt tttcagaacc gaaagtatac gaaggtgaaa ccattgagaa
     6421 atttattcct ggtatacatg ctccaaaatt acctgagaaa tcagtactag cagacgatat
     6481 tgagcgattt acccattttt cagccgggta tattggcatt caaccagaac gccctcattt
     6541 actgattgat gtgcgctatt cgaatttacc aacaagcact gacccgttat gggcgattgt
     6601 gattgattta catcgaccgg atcagcatgc gcaataccaa ttattccgag atgcatcgca
     6661 tgagattagg caaaagtttt tagcatttct acttggggaa catattaatt agaaaagtat
     6721 taggcgagtg ggattaatac tagcgtaagt agcgagaaca cgtgctttta acggcaagca
     6781 cgtgtttggt tcatcagtag gacattactt tataacatta acactgactg attttgctga
     6841 tgaattaccc gcagcatctt ttgctattgc ttttatcgtg tgcatgccag ccttaacata
     6901 cttagaacgt gtattccaac tcatacttag tgcacttcct atccccgttg tttttaggac
     6961 accatcaata taaattgaaa gagtgatacc gcttgcacca ttattatcgg atgcatttgc
     7021 cgtaatggtg actgctccag atacattacc tgttagtgga ttaataatgc taatgatagg
     7081 tgcttcagta tccacgatag gcggttgaat gggattacta actgtaacgc tgatcgcatt
     7141 ggatagcgac atattgcctg cggcatcata agcacgaaca accaaattcg tttctccatt
     7201 tgatgcacca gcagagtccc aggcaaatgc aaatggttca gaagtatcaa cggcaacact
     7261 cgtattgttg acccaaagct cagctcgtgt tacaccaaca ttatcataag cttgaatatc
     7321 aacgggaacg attccactta cagtttcccc ggaaagtggt tcaatagtcg atacgattgg
     7381 tggcgttgtg tcggttgctg ttatatttga cttagctgcc tgaactgcgg cggcagcatt
     7441 tacacggcca tatccataat aggggtcgcg acctgccgag ccgagatctt ttgctgttga
     7501 aaacaataag ctttcaatct ctgtgctacc cagctttggg ttggctgcca tcattaatgc
     7561 tacagtacct gctacgactg ggcttgccag tgaagttccc caagctgttc cgtataatgc
     7621 attgcggtag gtagtccaga tatcaatacc tggagctgat aatgcgacat aattaccaaa
     7681 actagaccaa ctggttttag tatcagttgc attggtagct gatactggga tcattgatgt
     7741 ggttggcgta aaaccttcgt cttttccacc attacctgca gcaacaataa ctagcccccc
     7801 tttatttttc atatattgtg cagcattaat gattgtggaa cttgctggta cattcgcata
     7861 gctaatgttt gccacacgtg ctccttgatc ggctgcccaa gttaggcctt gtgcgatagt
     7921 actaccataa gctgttgctt cagggctagc gattcttaca ggcatgattt ttgattgccc
     7981 agccatcgct gtaacaccaa ctccattatt gctggaggct gctgctgcac cagcaaccgc
     8041 agtgccatgc ccatttacat cgacagtatt ggagttatta tcatagaaat tccagccagg
     8101 gactattttt cctgctagat ctggatgtgc gctgtcaacg ccagtatcga gaatggcgat
     8161 agtgatgcct gccccttgtg aaagatccca ggcagcagta gctccagttt tggaaaggtg
     8221 ccatgcgttg ccaaagtatg ggtcattggg agtaaggcta gagtagaaaa tataatcagg
     8281 ctctgcatat ttcaaatacg gatgctgatt taaagcagta atgacacttt cctcagaata
     8341 ttccggtata tcaacaatat ataaatcact ctggcctatt tttctggctt taccattatg
     8401 ctctttcaga atatgagcca gagccttagc aggcaaccct gctcgaggca ttactaaaat
     8461 cctacctctt gctgatgttt ctgcattcca tttttgacga tttcctttct caagtgattc
     8521 gcttgaattt gcatgttctg ggggagatga atgcaattgg gtggggatca aaagaaatga
     8581 taaaaataag catacaaaaa cagaaaaact aagaaagtcg taaatttgat tttttgatgc
     8641 actgaaaaat aaataacttt gattggtgct ttccatgaat ttatccttta cttttttaaa
     8701 ttcaggggaa gcccgggaaa ggaactgcat gagtcgttgc gttgcatggc ttgctagttt
     8761 tatataaaaa gcgagttgca gcgcttgaac tgatcttcga attcaaacaa ttgcgagtgt
     8821 ttgcttaatg aagaatacta tctggcagtc ttgctatcct ggggaatttt ccctgtagat
     8881 caatgacttt gcgtccctca ctttcgcgag gtttgccttt aacaaatagt tgtttgctgt
     8941 aagtagtact cacagacaaa aaaactatcg aatatttatc ggatagattg gaagaatagt
     9001 aaagaagtca cttgctgata aacgagtcca gcgttgtgag atttggtttt tgttgagaat
     9061 gaaatacaaa gtaagtggag tagcgctact aatgatttga tttgctagat aataccgggg
     9121 aagaatcggt atttaacttt ctgacaatat agatcgtatt cgttatctac actgcgcaaa
     9181 tgatcttctt ccgttaatgc gcgtaggata taaatgattg accaacccag catagatcca
     9241 acggcaagca aagttgctgt tatggaagtt tctgttgcag cgattactgc gggtaatacg
     9301 ccaatccacc aagctaaatt tttacacgtg taagctggat gacgaatata gcgataaggg
     9361 ccgtgcataa taattcctcg atgcgtcaaa ttacttgcct tgagattaag tgcaatcgaa
     9421 gcccaagtat agaaagccat taagattaat aaaaaaatat ttaaagcgat atgtaaaatc
     9481 ggatcattaa attgaggaaa atccgtggta tatccagcaa aaacagaaga agttagaccg
     9541 ttgaaagggg ggtagctggc aagtgctaca aaccatccca ttaaagtagg gtcgacagat
     9601 cggatttcat ttttaagtga tggcaactcg atcatgtagc caaccgtaaa gaacagaaca
     9661 tctaaaaaga gaattacttt gaacatgaac caaaagccat gtgcattaaa taacgatatc
     9721 cattcgatgg ttgcttgatt taatgctacg gctaatttat ttccatgctc gatcatatgt
     9781 cctgtgtgag caaataacca aacgaccatg agcgggataa agaacccttt aagtaggata
     9841 ctcagtaatc caagtctttc ttcaaaaggt agtcctgtct gcaaaatgct tttgggtgac
     9901 ctcatcagac gttggattgc acgtaggcag taaattgatt tagaaattct tggtgatttt
     9961 tccgtcaaat aaaaaataga gagaataaca acataagcta acattgccgt cagcagaact
    10021 tgccagtgac tatattcttg gccaagaaag tcggttgatg aagaaaagta gttttgataa
    10081 taaggtacgg ttccgtaaaa agtacctaaa atcaatatta aaaatagagt tgctgataaa
    10141 tttctgacgc gttgtctcat tgacttctaa taggtaatta aagctcaaac aaaatagcat
    10201 taatttaatt tgtgcaatac aaaaagaaaa tgcgcctaag cgcattttct ttaaaccttt
    10261 catttaaatg ctacaaacta tttcgtagta attaaaatta agctagctac taataagttg
    10321 tcagcgttta ttttgatcct aggcgacggc gtcttgctac aaacatgagt gcttgcccga
    10381 gtaaaccaat gcctaataat gctaaaacac ctggttctga aacagtattg ccattgcttg
    10441 tgccaccact ggtactactc gtggttgtac caccactcgt gctactggtt gaggttccac
    10501 cgctggtact gcttgttgat gtaccaccac tggtgctcgt gctactactg ctggtaacaa
    10561 ttttatcgct tccgttacca tttgcgccgg tacgctgtgt ccgcaatata gagtcatccc
    10621 caacatcact aaagttccat ggattcggat cggagatttg taatactaat gttgcgatat
    10681 cgggatatgt gttagccgtg ctggttgcaa ctccagaatt gtaaataccg ccaataatgc
    10741 cattattcgt cgtgaaagcg atgtcaaaca cagtattacc cggggtgata ttgttattta
    10801 cattaaaacc aatattccag aggctggcca gattagtacc attgccatcc acaaatgata
    10861 aaactgatgc gttattaata ttttgagtaa tattaaaaac gatcccggtg atgattccac
    10921 tgaaatttga agtgttatca aaagtaattt tgagttgatt tgcgccaaca ccttcattgc
    10981 taaatgtaac aataccaccc gactgagaag tggtacccca aaaattctga gtttcaacat
    11041 aagcgtaggc tgcacttgcg ctcatcgcaa tagctactgc gcttgcgaca cccgctactt
    11101 tgtttttaaa agatttattc attgattgct tccctcatta atctaaatgc ctatgaacta
    11161 ttctcagtgt ttttcatctt atatagcaag atcaata
//
LOCUS       DAOQKQ010000083        11099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_2761_length_11099_cov_6.169775, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000083 DAOQKQ010000000
VERSION     DAOQKQ010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 11099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11099
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2761_length_11099_cov_6.169775"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..940)
                     /locus_tag="PKZ49_06860"
     CDS             complement(107..940)
                     /locus_tag="PKZ49_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HND36320.1"
                     /translation="MRTTLRLIILGISIYAAWAIFIFFAQSHLIYHPEIDREIVNTPD
                     QFSMPYESVVLTTSDQEKLHGWFVPAAKETTATILFLHGNAGNISHRMGYLSIFHQLG
                     YNTFIVDYRGYGNSSGTPTEEGTYQDALTAWNYLIENKTIPGSHIVLFGESLGGAIAA
                     WLASKNDPGALVLASTFTSIPDIAADLYPLLPVRLMARFHYNTSKYLQSVTCPVIVAH
                     SPEDEIVPFKHAQALFDKAPNPKQFIVLQGKHNDSFFVEQDAWVHPLNQFIRASLSAY
                     K"
     gene            1032..1580
                     /gene="ppa"
                     /locus_tag="PKZ49_06865"
     CDS             1032..1580
                     /gene="ppa"
                     /locus_tag="PKZ49_06865"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008060184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HND36321.1"
                     /translation="MNLDRVDSGRDLPNDFNVIIEIPMNSEPIKYEMDKKTGAMFVDR
                     FMSTAMRYPCNYGYIPHTLSEDKDPVDVLVISPTPLITGVVVRCRPIGLLNMTDEAGE
                     DVKLIAVPIDKLCGLYIHVQTLDDLPEFMRAQIAHFFAHYKDLERGKWVKIEGWGDVE
                     AARAEIMTGVKRYNSAKNKPKF"
     gene            complement(1622..2800)
                     /locus_tag="PKZ49_06870"
     CDS             complement(1622..2800)
                     /locus_tag="PKZ49_06870"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HND36322.1"
                     /translation="MNSYDVIIIGAGAAGLMCAIEAGKRGRSVWLIDHMAKFAEKIRI
                     SGGGRCNFTNIFTQPQHYLSQNPHFCKSALAQFTPHDFINLVNKHHIAFHEKKLGQLF
                     CDHSSQQIIDMLLSESHQAKVSFALQTRVTQIEALADGYRLDTNRGIHTCRSLVIATG
                     GLSIPKIGASNFGYEVAKKFDINVIKPRPGLVPLTFDGTLLEQCKSLSGVSIDATVQF
                     NRQSFSEGLLFTHRGLSGPSILQISSYWEQGKAIRVNLIPDIDLYSVLKAKKNNQPKL
                     HIGGVLAERLPKRLAQNLCDQYVFTGAIGILSDRQLAKIAEVIQAWLIWPTGTEGYRK
                     AEVTIGGVDTNELSSRTLEAKKRPGLYFIGEVVDVTGHLGGFNFQWAWSSGFAAGQSV
                     "
     gene            complement(2894..3376)
                     /locus_tag="PKZ49_06875"
     CDS             complement(2894..3376)
                     /locus_tag="PKZ49_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36323.1"
                     /translation="MINKSIIVLALFLVACATTAPKEKPKIFEDQLAGSYSNLARCTI
                     AKLRAEGNWALRSLQFNLKHYKDIEASEVFAYDIRFLPGVFARNSPANPDAVFDYIDP
                     NPSITTYEQRLTNILPGYAFALLIKKNDDTTVNATLEGEMRSGEIAWKHLLECPAKAE
                     "
     gene            3700..4533
                     /locus_tag="PKZ49_06880"
     CDS             3700..4533
                     /locus_tag="PKZ49_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HND36324.1"
                     /translation="MEAEHGELIFPTSVKKAIEVKRILDDPARDLDAAARLIRSDPLL
                     SARVVAVANSTAFNRSRKTITNVPAAITLLGLFLVKSLILGVAVRQIAESATTAKREL
                     IAQLWKHSAYVGSIAQVIARRITKHDPETALFAGMIHEICWFYLLFRTKDYPDLFEGD
                     ISSLWNKDDEIDNEGEFIGEIEVGTLLLKALSIPDSIIDTIAFVWRGYQTFPPTSLGS
                     VLLIANQLAPIQSPFTLPIYQTNQDHLVKLDLFFKDYSLSEILAESEEEVDSLTNALC
                     L"
     gene            complement(4541..5677)
                     /locus_tag="PKZ49_06885"
     CDS             complement(4541..5677)
                     /locus_tag="PKZ49_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HND36325.1"
                     /translation="MEKEQAIKFMHDLLRLMLNKKASDLFITAGYPPAMKIDGKMTPV
                     SQQQLTAQHTVELVRAIMNDKQVGEFEASKECNFAIHPQGIGRFRVNAFIQQQRAGVV
                     IRTITTQIPKFDDLKLPEVLKDVVMSKRGLVIFVGGTGSGKSTSLAALIGHRNQNSYG
                     HIITIEDPVEYVHEHINCIITQREVGVDTDSWEAALKNTLRQAPDVILIGEIRDRETM
                     EHAIAFAETGHLCMGTLHANSANQALDRIINFFPEERRAQLLMDLSLNMRALIAQRLI
                     PKKEGKGRVAAIEVMLNSPLIADLIFKGSVHEIKEIMKKSRELGMQTFDAALFDLYEA
                     GLISYEDALRNADSMNELRLQIKLNGSETKNKNVSAGIEHLTIT"
     gene            complement(5711..6754)
                     /locus_tag="PKZ49_06890"
     CDS             complement(5711..6754)
                     /locus_tag="PKZ49_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HND36326.1"
                     /translation="MNIVELLAFVVKNNASDLHLSAGMPPMIRVHGEIRRINLPAMEH
                     KDVHDMIYDIMNDSQRKHYEEHLECDFSFAIPNLARFRVNAFNTQRGAASVMRTIPSK
                     VLSLEQIKAPKIFAEIAQQPRGVVLVTGPTGSGKSTTLAAMINDINESNYGHILTLED
                     PIEFVHESKKCLINQREIGRDTHSFANALRSALREDPDIILVGEMRDLETIRLAMTAA
                     ETGHLVFGTLHTSSAAKTIDRIIDVFPAEEKEMVRAMLSESLRAVISQTLLKTKDGNG
                     RVAAHEIMIGTPAIRNLIREGKVAQMYSTIQTSQAVGMQTLDQNLADLVKRNVVSNAE
                     ARTKAMNKDNFRG"
     gene            6948..7664
                     /locus_tag="PKZ49_06895"
     CDS             6948..7664
                     /locus_tag="PKZ49_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HND36327.1"
                     /translation="MDTIATRLESVKLRIAEAAKLAGRDPQEILLLAASKTNQPEKLR
                     EAWNAGQKVFGENYLQEGLIKIHALSDLPIEWHFIGPIQSNKTKPIAENFAWVHSIDR
                     EKIAARLSSARPESLPPLQVCVQVNVSGEVTKSGIAPEDAIALAKFVSQQPRLKLRGL
                     MAVPELTSATALQREQFQIMRNIFDQLKNQGFDVDTLSMGMSEDMNIAIAEGATIVRI
                     GTAIFGPRRYAIPEELAPRQ"
     gene            complement(7633..7902)
                     /locus_tag="PKZ49_06900"
     CDS             complement(7633..7902)
                     /locus_tag="PKZ49_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HND36328.1"
                     /translation="MALIITDECINCDVCEPECPNGAISQGEEIYEIDASRCTECVGH
                     YDKPQCIEVCPVNCIVTNPDLPESKDQLLAKYHRLITGEGQAPQE"
     gene            complement(7918..8397)
                     /gene="coaD"
                     /locus_tag="PKZ49_06905"
     CDS             complement(7918..8397)
                     /gene="coaD"
                     /locus_tag="PKZ49_06905"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HND36329.1"
                     /translation="MNKVIYPGTFDPITRGHEDLVRRASTLFEEVVVAVAGDTTKTPF
                     FSLEERISMAKTVLSDYANVKVIGFSGLLMSFVQRQNSRIIVRGLRAVSDFEYEFQLA
                     GMNRSLYPDVETVFLTPSEQYMFVSATIVREIARLGGEADKFVHPIVAEQLRRKLEH"
     gene            complement(8366..8941)
                     /gene="rsmD"
                     /locus_tag="PKZ49_06910"
     CDS             complement(8366..8941)
                     /gene="rsmD"
                     /locus_tag="PKZ49_06910"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HND36330.1"
                     /translation="MPQSGKVRIIGGQWRSRLISVANEVGVRPTPNRVRETVFNWLGQ
                     DLQGKHCIDLFAGSGAMGFESASRGAESVVMVEIKDNVFQVLRSMKEKLGAAQIKLVK
                     MDAIEFIRSDQNQYDVIYLDPPFQLGLLPKILPLLPSRLSKSGIVYLESEYPFQQNDH
                     WVILKCGRAGNVFFQLLKFEDHEQSNLPGHI"
     gene            9198..10445
                     /locus_tag="PKZ49_06915"
     CDS             9198..10445
                     /locus_tag="PKZ49_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HND36331.1"
                     /translation="MSPYELLIGLRYTKAKRRNHFISFISLISLLGITLGMTALITVM
                     SVMNGFHKEVRSRILGVASHVQITSFNGTIAHWEQVADIAVKNPQVVSAAPYVNAQGM
                     LSYNQVVRGVIVRGILPDAENTVADFSNMMMMGELNQLQAGEFGIVIGLELARALGTF
                     PGDKIVLISPQGQVTPAGILPRLKQFTVIGVFEAGHYEYDSGLALIHLVDAQKLYRME
                     PDQVSGVRLKLHDLFEAPKVVRELSALFSSDTYISDWTQQHTNYFRAIQIEKRMLSLI
                     LALIIAVAAFNIVSTLVMAVTDKQSDIAILRTLGASPGSIMKIFIVQGTLIGLFGTLL
                     GIIGGVILAYNVSDVVVFIEQMFNIQFLSQEVYYISEIPSDPHLSDIVTVAVVSFVLT
                     LLATLYPSYRASKVNPAEALRYE"
     gene            10438..>11099
                     /gene="lolD"
                     /locus_tag="PKZ49_06920"
     CDS             10438..>11099
                     /gene="lolD"
                     /locus_tag="PKZ49_06920"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HND36332.1"
                     /translation="MSNQVIACQNLYKSYFQGKLEVPVLHGVNLQLNKGEMVSIVGAS
                     GSGKSTLLHLLGGLDSPTKGQIKILDQDITQIDEQQRCQLRNQSLGFIYQFHHLLPEF
                     SAQENVAMPLLIRRMNRKEAMDTAAEMLQQVGLGHRLTHTPGELSGGERQRAAVARAL
                     VTRPACVLADEPTGNLDRHTAEGVFNLMLDLNQKANASLIIVTHDSQLAQRAERILHL
                     VDG"
BASE COUNT         3240 a         2318 c         2282 g         3259 t
ORIGIN      
        1 ctgtcatcct aaatacccag gttgggctcc ttggtgcgga aagtgaagcg caacgattac
       61 aaaatttgct ggtagcaaat tcgagtaata atactaatac ttagcgttat ttataggcag
      121 ataaactggc acggatgaat tgattcaaag gatgaaccca tgcatcttgt tcaacaaaga
      181 agctatcatt gtgctttcct tgcaacacga tgaattgttt gggattaggt gctttatcaa
      241 aaagtgcttg ggcatgcttg aagggcacaa tctcatcctc tgggctgtga gcaacgatga
      301 cgggacaggt aacagattgc aaatatttgc tggtattata gtgaaaacgt gccatgagtc
      361 gcacaggtaa taaaggatat aaatcggctg ctatatctgg aatagaggta aaggttgaag
      421 ccaacactaa tgcgcccgga tcatttttcg atgcaagcca tgcagcaatg gctcctccca
      481 gtgactctcc aaacaagacg atgtgcgaac ccggaatcgt cttattctcg atcaggtaat
      541 tccatgcggt aagtgcgtct tgataagtac cttcttcagt tggggtaccg ctactgttac
      601 cgtacccccg ataatcaaca atgaatgtgt tatagccaag ttggtgaaat attgaaaggt
      661 agcccatgcg atgagaaata ttgcctgcat tcccatgcaa aaaaaggatg gttgcagttg
      721 tttctttggc tgcaggtaca aaccacccat gtagtttttc ttgatcgcta gtcgtcagta
      781 caactgattc ataaggcatc gagaattggt cgggagtatt gacgatttca cggtcaattt
      841 ctggatggta aatgagatgc gattgagcga agaaaatgaa tatcgcccaa gctgcataaa
      901 tggagatgcc gagtatgatc aaccgaagtg ttgtgcgcat acggataaca aagaaagaac
      961 ttttcttatc aatccaagta aattagaaac ttgctattat aatcctgctt taactctctt
     1021 taggatttgc tatgaactta gatcgtgtag actcaggtcg ggatcttccc aatgacttca
     1081 atgtaattat cgaaattccg atgaattccg agccaatcaa atatgagatg gataaaaaga
     1141 cgggcgccat gtttgtcgat cgcttcatgt caaccgcgat gcgctatcca tgcaattatg
     1201 gttatattcc gcacactttg tcggaagata aggaccctgt tgatgttcta gtcatttctc
     1261 ccactccgct cattactggt gtcgttgtgc gttgtaggcc gattggttta ttgaacatga
     1321 cggatgaagc cggtgaagat gtcaagctaa tcgcagtacc tattgataaa ttatgtgggc
     1381 tttatataca tgtgcaaacg ctggatgatt tacccgaatt catgcgcgca caaattgccc
     1441 attttttcgc tcattacaaa gatttagagc ggggcaagtg ggttaaaatc gaaggctggg
     1501 gagatgttga agcagcccga gccgagatca tgacaggggt aaagcgctac aattccgcta
     1561 agaataaacc taaattttga ttgctagtgt taataattta cctgggtaaa tcgtatgaca
     1621 gctagactga ttggcccgca gcaaagcccg atgaccaggc ccattgaaag ttgaatccac
     1681 ctaaatgccc ggtgacatcg acaacttcgc caataaaata aagccctggt cttttttttg
     1741 cttccagagt ccgagaagaa agttcattag tatcgactcc accgattgtg acctctgctt
     1801 ttcggtaacc ttcagtacca gtaggccaaa tcaaccaagc ctgaataact tcagcaattt
     1861 ttgcaagctg ccggtcggac aaaataccaa tggccccagt aaaaacatat tggtcacata
     1921 aattctgggc gagacgttta ggtaatcgtt cagcgagtac acctccaata tgtagttttg
     1981 gttggttatt ctttttggct ttgagcactg aataaagatc gatatcggga ataagattga
     2041 ctcgtatcgc ttttccctgt tcccaataag aagagatttg caaaatcgat ggcccactta
     2101 atcctcgatg ggtaaacaat aacccttccg aaaaggactg gcggttgaat tgtaccgttg
     2161 catcaatcga aacaccgctt aaagatttgc attgctccaa taaggtgcca tcaaatgtta
     2221 atggtacgag cccagggcgt ggtttaatca cattgatatc aaatttcttt gcaacttcat
     2281 acccaaaatt agacgcgccg attttgggga tagataatcc ccccgttgcg atcaccaagg
     2341 aacgacaggt atggataccc cggttggtat ccagtcgata accatcagcg agcgcctcaa
     2401 tttgcgtgac acgtgtttgc aacgcaaacg atacttttgc ctgatgcgat tccgatagca
     2461 gcatatcaat tatctgctgc gatgaatgat cgcaaaaaag ctggcccagt ttcttttcgt
     2521 ggaaagcaat gtggtgctta tttacaagat taatgaaatc atgaggcgta aattgagcga
     2581 gtgccgattt gcaaaaatga ggattttgag aaaggtaatg ctgtggctgg gtgaaaatat
     2641 tggtgaaatt gcatcgtcct ccaccagaaa ttcgtatctt ctctgcaaac ttagccatat
     2701 gatcgataag ccatacactt cgacctcgct tacctgcttc aatggcacac atcaaaccgg
     2761 ctgcacctgc accgatgata attacgtcat atgaattcat tgatccagtt gatcgattat
     2821 gcgttcgagt acactcgatc agctttaatt acattaaaaa ataaaggtaa attacttagc
     2881 gagatagcaa aagctattct gcttttgctg gacattcaag gaggtgtttc caagcaattt
     2941 cacccgagcg catctcacct tctaatgttg cattgactgt cgtgtcatca tttttcttga
     3001 tgagtaaagc aaacgcatag cccggtaaga tattcgtcag cctttgttca tacgtcgtaa
     3061 tactaggatt agggtcgata taatcaaaga ccgcatcagg attagcgggt gaattacggg
     3121 caaaaacacc gggtaagaaa cgaatatcgt aagcaaagac ctcagaagcc tcaatatcct
     3181 tgtagtgttt caaattaaac tgtagcgaac gaagcgccca gtttccttca gctcttaatt
     3241 ttgcgatagt gcagcgagct aaattcgaat aactcccggc caactgatct tcaaagattt
     3301 ttggtttttc ttttggcgca gtcgttgcac atgccaccaa gaacaaagct aaaacaataa
     3361 ttgatttatt gatcacgatt attactccaa aaaaaataga cagagcgaga atgaattact
     3421 ttattgatat cagaagaaaa tttgaagact gaatagtaaa attctaactt actttaagtg
     3481 ttattctcat ggccttactc attctaatga gctcccttag ttgagattaa aagttccccc
     3541 aatatctgat tagcaaaatc catgctaact tactgattaa aagtaattta ctaaattaat
     3601 aaattgttaa accgataccc taaaaggtgg tctaattgtt aactattaat tctaactgca
     3661 tttaccatgg ataaagctga gatcttaaat agaattaaaa tggaagcaga gcacggggaa
     3721 ttaatttttc caacttcagt aaaaaaagca atagaagtta aaagaattct ggatgaccca
     3781 gcccgtgacc ttgatgctgc tgcccgactt attagatctg atccattatt gtcagcaagg
     3841 gtagtcgctg tcgcaaattc cacagcattt aatcgatcaa gaaaaacaat caccaatgta
     3901 cccgctgcaa taacattgct gggactattt cttgtaaagt ctctgatttt aggggttgca
     3961 gtgcgccaaa ttgcagagtc tgcaacaacc gccaaacgcg agttaattgc tcagttatgg
     4021 aaacactctg cctatgttgg ttcaattgct caggtcattg cacgaagaat tacaaaacat
     4081 gatcctgaaa ccgctctgtt tgctgggatg attcatgaga tatgctggtt ctacttacta
     4141 ttccgaacaa aagattaccc cgacttattc gagggtgata tttccagttt atggaataaa
     4201 gatgatgaga tcgataatga aggtgaattt attggtgaaa ttgaagtagg tacattgcta
     4261 ttaaaagcgt tgagtatccc tgattccatc attgatacca ttgcttttgt ttggcgcggc
     4321 tatcaaacct ttcctcccac ttcactgggt agtgtcctcc taatcgctaa ccagctggca
     4381 cccattcaat ccccttttac actccccatt tatcagacaa atcaagatca tttagtcaaa
     4441 cttgatttat tctttaaaga ctattcgctt agtgaaatac tagcggaatc tgaagaagaa
     4501 gtagattcat taacgaacgc cttatgctta taagcttgca tcaggtaatc gttaaatgct
     4561 caataccggc gctgacattt ttatttttgg tttctgaccc attgagtttg atctgtaagc
     4621 gcagttcgtt catagaatct gcatttctaa gcgcatcttc ataactaatc aagcccgctt
     4681 catatagatc gaatagtgct gcatcaaaag tttgcatacc tagctctctc gattttttca
     4741 tgatctcttt gatttcatgg acgcttcctt tgaaaattag atcagcaatt aagggtgaat
     4801 tgagcataac ttcaattgct gctacacgcc ctttaccttc tttcttagga atcaatcgct
     4861 gcgcaataag tgctcgcata ttgagggata aatccatgag tagttgagca cggcgctctt
     4921 ctgggaaaaa gttaataatt cgatccaggg cttggttagc actgtttgca tgcaatgtcc
     4981 ccatacatag atggcctgtt tcagcaaatg caatggcgtg ttccattgtt tcgcggtcac
     5041 gaatctcccc gattaagata acatcagggg cttgacgcag cgtatttttt aacgcggctt
     5101 cccaggaatc ggtatctacg ccaacttcac gctgggtaat aatgcaattg atatgttcat
     5161 gaacatattc aactgggtct tcaatggtga tgatatggcc ataactgttc tgattccggt
     5221 gaccaataag cgcagccaac gaagtcgact taccagagcc agtgccgcca acaaagatga
     5281 ctaatcctcg cttgctcatt acgacatctt tcagtacttc aggtaacttc aaatcatcaa
     5341 atttcggaat ctgcgtggta atcgttcgaa tgacaacgcc tgctctttgt tgttgaataa
     5401 atgcattgac gcggaagcgc ccaatacctt gtggatggat tgcgaagtta cattctttgc
     5461 ttgcttcaaa ttctcccact tgcttatcgt tcatgatagc tcgtacaagc tctactgtat
     5521 gttgcgctgt aagttgctgc tgtgatacag gggtcatttt tccatcaatt ttcattgcgg
     5581 gaggatatcc agcggtaatg aacagatcag aagctttctt atttaacatt aagcgcagta
     5641 aatcatgcat aaactttatt gcttgttctt tttccataat tttatctact gataatgatc
     5701 aattaaggtg ttatccacgg aaattatctt tgttcatagc ctttgttctt gcttcagcat
     5761 ttgagacaac gtttcttttt acgagatcag ccagattctg gtcgagggtc tgcataccaa
     5821 ctgcctgact cgtctggatc gttgaataca tttgagctac tttcccttct cgaattaggt
     5881 tacgaatcgc aggagtacca atcatgattt cgtgtgctgc aactcgacca ttaccatctt
     5941 tagttttcaa taaagtttga gaaattacag cacggagcga ttctgataac attgcccgaa
     6001 ccatttcttt ttcttctgct ggaaaaacgt caataatacg atcaatcgtt ttagcggctg
     6061 agcttgtgtg tagggttcca aagactaggt gcccagtttc tgcggcagtc atagctaagc
     6121 gaattgtttc caaatctcgc atttcaccga ctagaataat atctggatcc tcacgcaaag
     6181 ctgaccgaag cgcattagca aaactgtgag tatctctacc aatctctctt tgattaatta
     6241 agcatttttt actttcgtgt acaaactcga ttggatcttc aagtgtaaga atatggccat
     6301 aattactttc attgatatca ttgatcatcg cagcaagtgt ggtcgattta ccagaaccgg
     6361 ttggtccagt tactagtacg acaccgcggg gttgttgcgc gatttcagca aaaatcttgg
     6421 gggccttgat ttgctctagt gatagtactt tggatggaat cgttctcatt accgaagctg
     6481 caccccgttg tgtgttaaag gcatttaccc ggaagcgagc gagattggga atagcaaaag
     6541 aaaagtcgca ttccaaatgc tcttcataat gctttctttg actatcattc atgatgtcgt
     6601 agatcatgtc atgcacatct ttatgctcca ttgcaggaag gttaatgcgt ctgatttcac
     6661 catgaacacg aatcatggga ggcatgccag cagaaaggtg caaatctgaa gcgttattct
     6721 tgaccacgaa ggcgagtagc tctactatgt tcatctatta ttcctagtcc aagttgttag
     6781 gttatactgt gattacttca gtatgtttta cccaattcca agtaaaatcc tgcttgttta
     6841 tcaacggtag gcctaaacaa aaattgagaa gctactgcta gcgtagttaa tttagaattt
     6901 tccactccaa atacttacat tttcgtttta ttttttactt catcactatg gatacaatcg
     6961 caacacgttt ggaatcagtt aaattgcgca tagctgaagc tgcaaagctt gcaggaagag
     7021 atccacaaga aattctatta ttagcggcaa gcaaaacaaa tcagccagag aaactgcgtg
     7081 aagcatggaa tgctggtcag aaagtatttg gagagaatta tttacaagag ggactaataa
     7141 aaattcatgc gctttctgat cttcccattg agtggcattt tattggccca atccagagca
     7201 ataaaacaaa accaattgct gagaatttcg cttgggttca cagtatcgat agagaaaaaa
     7261 ttgctgctcg gttgtctagc gctagaccag aatcattacc accgctacag gtttgtgtgc
     7321 aagtcaatgt aagtggagaa gttacaaaaa gcggcatagc cccagaagac gcgattgcat
     7381 tggctaaatt tgttagtcaa caaccacgtt taaaattgcg gggtttaatg gcagtacctg
     7441 aattgacttc ggccacggct ttgcaacgcg agcaatttca aatcatgcga aacatattcg
     7501 atcaacttaa aaatcagggc tttgatgttg atacactatc catgggcatg tcagaagata
     7561 tgaacattgc catagcagaa ggtgcaacta ttgtccgtat cggaacagca atttttggac
     7621 cgagacgtta cgctattcct gaggagcttg cccctcgcca gtaattaacc gatgatattt
     7681 ggcaagcaat tggtctttac tttcaggcag atcaggattc gtaacgatac agttcaccgg
     7741 acaaacctcg atacattgcg gtttgtcata atgcccaaca cattctgtac accgacttgc
     7801 gtcgatttca taaatttctt cgccttgcga aatagctcca ttagggcatt ctggttcaca
     7861 aacatcacag ttaatacatt cgtcagtaat aataagcgcc atattgtttt tatttaatca
     7921 atgttctagt tttctacgta gctgttctgc cacaatggga tgaacgaatt tatcagcttc
     7981 tccacctaat ctcgcaattt cccgaacaat cgttgcggaa acaaacatat attgttcaga
     8041 aggtgttaga aatacagttt cgacatcagg ataaaggcta cggttcattc ctgccaattg
     8101 gaattcatac tcaaaatcag agaccgcacg cagtcctcga acaataattc gagaattctg
     8161 gcgttgtaca aatgacatca atagcccaga aaaaccgatt actttcacgt tagcgtaatc
     8221 ggatagcacg gtttttgcca tactaattcg ttcttcaagt gagaaaaaag gtgtcttggt
     8281 ggtatcaccc gcaacagcaa ctaccacttc ctcaaacaaa gtcgaagcac gacggactaa
     8341 atcttcatga ccgcgtgtaa tgggatcaaa tgtgcccggg taaattactt tgttcatgat
     8401 cttcaaattt taagagctga aaaaaaacat taccagcacg cccacacttc aaaataaccc
     8461 agtgatcatt ctgttgaaat ggatactcac tctctagata aacgattcca gatttactta
     8521 atcgcgatgg taatagtggt aatattttag gaagtaaacc cagttgaaag ggtgggtcga
     8581 gataaatcac atcatattgg ttttgatccg atcggatgaa ttcaatcgca tccattttaa
     8641 ccagtttaat ctgtgctgcg cctaattttt ctttcattga gcgtagcact tgaaaaacat
     8701 tatcctttat ctctaccata acaacacttt ctgctccacg agatgccgat tcgaacccca
     8761 tggcaccact cccagcaaat aaatcgatac aatgcttacc ctgcagatct tgccctagcc
     8821 agttaaaaac ggtttcacga actcgattcg gtgtgggtcg aacaccaact tcatttgcaa
     8881 cagagatcaa acggcttctc cattgccctc caataatacg aacttttcca ctttgaggca
     8941 ttagctgata atatctattc tgaatggaca gaaaagcgct gatttaagta atttgagtta
     9001 cttaatcatc cgattgctag cattatactg aacaaaaaat aattgaataa ccactagcta
     9061 atcaatttaa ggcaatgaga aagcgtattc atcgtttaga aacagagcta gtttaatttt
     9121 aaaccatata acatcactat tgataatagc ttttcttaga aaacaacatc atctaaatct
     9181 ttttcaggaa ttcatttgtg tcaccttatg agctattaat aggcctacgc tacacaaaag
     9241 caaagcggcg taatcatttt atttccttta tttccttgat ttcactactg ggtattacac
     9301 tgggtatgac agcgctaatt acggtcatgt cagtcatgaa tggatttcat aaagaagtgc
     9361 gttcgcgaat attgggggtt gcttctcatg tgcaaatcac aagcttcaat ggaacgatcg
     9421 cgcattggga gcaggtcgct gatatcgcag tcaagaatcc tcaagtagtt tcggcagcac
     9481 cctatgttaa tgcacagggg atgctttcgt ataatcaagt ggtacgcggt gttatcgtgc
     9541 gcggaatatt accggatgct gaaaatactg tcgcagattt ctccaatatg atgatgatgg
     9601 gggagttaaa tcagctacag gctggagaat ttgggattgt cattggcctt gaattagcga
     9661 gagcgctggg tacttttcca ggtgataaga ttgtgttgat atcacctcaa ggtcaagtaa
     9721 cgccagcagg tattttaccc cgcttgaaac aatttaccgt tatcggtgtt tttgaagccg
     9781 gtcattatga atatgattca ggtctcgcgt taatccatct ggttgatgca cagaagctct
     9841 atcgcatgga acctgatcaa gtatccggtg ttcgcttaaa attacatgac ttgtttgagg
     9901 caccgaaagt agtgcgagaa ttgtcagccc tattttcatc ggatacctac attagcgact
     9961 ggacgcaaca acataccaat tactttcgtg caattcaaat cgaaaagcgc atgctgtcac
    10021 tcattctggc actaattatc gcagttgcgg cgtttaacat tgtttcgaca ctcgtcatgg
    10081 ctgtcacaga taaacaatcg gacatcgcca ttttacgcac gctcggcgct agcccgggta
    10141 gcatcatgaa aattttcatt gtacaaggaa cattgatcgg cttgttcgga accctattag
    10201 gcattatagg tggagttatt ctggcttata acgtcagtga tgtcgtagtt ttcattgagc
    10261 aaatgtttaa cattcaattc ctctcccaag aagtatatta cattagtgag attccttcag
    10321 atcctcattt atctgacatt gtaactgtag ccgttgtgtc atttgttttg acattgttag
    10381 cgacacttta tccaagttat cgcgcatcaa aagttaatcc tgccgaggca ttgcgttatg
    10441 agtaaccaag ttatcgcttg ccaaaattta tataagagtt attttcaggg aaagctagaa
    10501 gtacccgtat tacacggggt aaatctgcaa ctaaacaaag gtgaaatggt ttcgatcgtc
    10561 ggtgcttctg gctcgggcaa aagcacctta ctacacttac ttggagggtt agacagtcca
    10621 accaaaggtc agattaagat attggatcag gatatcaccc agatcgatga gcaacagcga
    10681 tgccaactac gcaatcaatc attgggcttt atttatcaat ttcatcattt acttcctgag
    10741 tttagcgcac aggaaaatgt tgcgatgcca cttttgattc ggcgcatgaa taggaaagag
    10801 gctatggata ctgctgcaga aatgcttcag caagttggac ttggtcatcg actgactcat
    10861 accccaggag aactttctgg cggtgaacgc cagcgtgcgg cggtagcaag ggcattggta
    10921 acaaggccag cctgtgtttt agcggatgaa cctacgggta atcttgatcg acataccgct
    10981 gaaggggtat ttaatttaat gctcgacctc aatcaaaaag caaatgccag tttaatcatc
    11041 gtaacacatg attcacagct cgctcagcga gcagaacgca ttttgcatct ggttgacgg
//
LOCUS       DAOQKQ010000084        10948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_2817_length_10948_cov_5.932434, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000084 DAOQKQ010000000
VERSION     DAOQKQ010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 10948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10948
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2817_length_10948_cov_5.932434"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..786)
                     /gene="pyrE"
                     /locus_tag="PKZ49_06925"
     CDS             complement(145..786)
                     /gene="pyrE"
                     /locus_tag="PKZ49_06925"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HND36333.1"
                     /translation="MSDYKKAFIELAIGCNVLRFGEFKTKAGRLSPYFFNAGLFNNGN
                     ALRQLGQFYAKAIIAAQLPFDMLFGPAYKGIPLVSTIAIALAELGYNYPFCFNRKELK
                     DHGEGGITIGAPLSGRVLIADDVISAGTSIGESVNLIRAESATPCGVIIALDRLEQGS
                     NKLSAVQEVNNLHHIPVISIIDLNDIVDYLKEATNMRQNLQAIQAYRERYGTQ"
     gene            807..1580
                     /locus_tag="PKZ49_06930"
     CDS             807..1580
                     /locus_tag="PKZ49_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HND36334.1"
                     /translation="MRIITLNVNGIRAAAKKGLFNWLPLQQADVICVQELKAGIDDMS
                     PEMKAFDGYQGYFHCAEKKGYSGVGVYTRNKPDQVIEGVGIPGIDSEGRYLRVDFGSL
                     SIVSIYLPSGSSGEHRQDAKFYFMEHFFPFLEEFSKEGREIILCGDWNIAHKEIDLKN
                     WRSNQKNSGFLPEERAWLSAVFEELKFVDVFRRLNSESEQYTWWSNRGQAWTNNVGWR
                     IDYQIATASIADKATQVSIYKQERFSDHAPLIIDYDYQL"
     gene            1577..2950
                     /locus_tag="PKZ49_06935"
     CDS             1577..2950
                     /locus_tag="PKZ49_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HND36335.1"
                     /translation="MKNTSLIAWLDACRIYLHPRVMGMLALGFSAGLPLLLILGTLSF
                     WLREAGIDRTTIGHLSWIGLAYGFKWLWSPLVDRMPLPILTKLLGRRRAWLLLSQITI
                     VIALIGMASTDPLLNLTHMVFFALAVAFASATQDIALDAYRIEAVAIELQGAMAATYQ
                     AGYRLAMILSSAGVLWIAASLDTMPTGYDYRAWQIAYFVMAAFMGIGVLTTLIIQEPA
                     VKISQLAVENEKRVEAMIARWNLNHRLAQLLLWFYGAVIAPFRDFIVRHRYNAFLILA
                     LIAVYRISDVVMGVMSNPFYVDMGYTKDEVATVSKVYGVLMTIAGAAVGGVLIMRIGI
                     IKTLFIGAILSAVTNLLFVWLTVIGHSLPGLIFTISADNLSAGIASSAFIAYLSGLTH
                     SAYSATQYALFSSVMLLLPKFIAGFSGQFVDAYGYAWFFLSTALLGLPVLILVWLVSR
                     IDFHSSE"
     gene            3152..4006
                     /locus_tag="PKZ49_06940"
     CDS             3152..4006
                     /locus_tag="PKZ49_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HND36336.1"
                     /translation="MQSKTITGLKNWISFFNKAEIPVLKLTGNELFLLLQQEKNPSAR
                     SVANIIKRDPLMTVKLLRYFCQHKHRSLEHEVMQAEQILIMLGVDKAMKNVLPEPTVE
                     ELLREQKVEVLINLLHLVHRSHIASNYAFEWAVRLHDTHFEEIRIAALLHDISELLMW
                     CFAPTEMLRIREMQKKDKTLRSYLAQKQVLGFTLADLQSELVLRWSLPELLITLMDDH
                     NASLQRVRNVVLAVNLARHSANGWDDAALPDDYTAIGELLRMPVHDVKIIVGAKNIEP
                     VNSQQSEH"
     gene            complement(4022..4888)
                     /locus_tag="PKZ49_06945"
     CDS             complement(4022..4888)
                     /locus_tag="PKZ49_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HND36337.1"
                     /translation="MITSMTGYATATQEISSGLISMELRSVNNRYLDIQFKLPDEFRS
                     FEAGMRELLNTKIKRGKIDCRLTFQSYPDLNLPQQLNSQVIRQLLDLNNTVQSNFIAA
                     RSLSVAEILKWPGILVSDSSPTTDLQEACNNLLQITLTELVSTRLREGEKLKAILIDR
                     LTQMRQLTSALSPRIPTLIKNFQEKLILRLQEAQIDCNDDRIRQELVLFASKIDIDEE
                     LSRLNTHLDEVEQTLLKGGASGKRLDFLMQELNRESNTLGSKSLDVEVSKISVELKVL
                     IEQMREQVQNIE"
     gene            complement(4942..8718)
                     /gene="hrpA"
                     /locus_tag="PKZ49_06950"
     CDS             complement(4942..8718)
                     /gene="hrpA"
                     /locus_tag="PKZ49_06950"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HND36338.1"
                     /translation="MSISQHLSAENLARRSNNLPKPKYSDELPICKQRHKILDAIRSN
                     SVIIVSGETGSGKTTQLPKFCLELGRGVSGLIGHTQPRRIAARSVAIQIATELSSPLD
                     HAVGYKVRFSDNINQDTYIKLMTDGILLAETQSDPYLQKYDTIIIDEAHERSLNIDFL
                     LGYLKQLLPKRADLKLIITSATIDAERFSHHFNNAPVIEVSGRTYPVEIRYHAKDNNQ
                     DEEEYDLQQSILDATDELLRTGIGDILIFLPGEREIKETSETLRKHRFNHFSDPGKLE
                     ILSLYARLSYAEQQRVFHKSNAVSRIILATNVAETSLTVPGIRYVIDTGVARINRYNH
                     RNKIEQLLVEKISQASANQRAGRCGRVMSGICIRLYAKEELETRPLYTDPEILRTSLA
                     SVILRMKSLKIGDIENFPFIQPPLPRMIADGYQSLSELGALDIHQKLTTIGWQLAKFP
                     IDPKIARMILAAKQENCLSEVLIIASVLSLADPRERPFDQQEAADRAHLPFHDERSDF
                     IGFLKLWNFFDELLKHKKSKKKLITQCHEHFISYRKMREWREIHGQLHTLVLEFGLRP
                     NQVPANYDEIHRALLAGLLSNIGFKSENEGEYLGARGIKFFIYPGSTLRKAKAKWIVA
                     AELVETTKLYARCAATIDVTWLEKIAGKLCKKHFFDPHWEKKTAQVMVYERVTLYGLM
                     IVPKRSVTYGKMNPKEAREIFIRNALVLGDYETNAQFLKHNRQLIQDIEELEHKTRRQ
                     DVLVEEQDIFTFYDKIIPDNILNGSSFESWRKQVEQDNPHLLYLSREYLMRHKADTAF
                     ETLFPESISIFDQQLPLTYRFEPGHAMDGVTVTAPLHSLNKLKANQFQDLVPGLIREK
                     ITWCFKALPKPIRRNLIPIAEHVTHFLDYRDKQKHSLSLLEALSEYIFSITGTKISSD
                     TWDTKLMPLHLQMNYRIIDDAGKELGVNRDLIELQDQFGQTAQLIFSQNNAANQLGIE
                     RDQITQWNFGQLPEEVTFIRAQKQLTGYPALVDQIDHVAIRLFDTIEVAHNHMRSGVK
                     RLLILELKDPIKLLEKKLPSTKSDSLVLSHLIDSETLKQDMFAAIYDRALIGDEPLPR
                     NEHEFSQLKQKGRIRLNSVADTLIQFIHDLALEYQAFKQKLSTVNPHHAQIKQELNNQ
                     LQALIYPNFLQQTDWVRLPHLLRYLKGMLVRLDKYPNNPARDNQHALAISVLWNQYRQ
                     RYEKHSKRGFKDPKLEEFRWQIEELRISLFAQELKTPYPVSVKRLQKLWEQVHE"
     gene            complement(8797..9267)
                     /locus_tag="PKZ49_06955"
     CDS             complement(8797..9267)
                     /locus_tag="PKZ49_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HND36339.1"
                     /translation="MDNIWFKPYTLDYLEGLRNANMGIHIGIHFSEIGPNFLKARMPV
                     DQRTTQPFGILHGGASCVLSETLGSVSAWMTIDPEKYRAVGLEINANHIRAVTKGYVI
                     GICTPLHVGRRTQVWQTDITEETTGKRVAISRLTVAIIEQGTLSNQKETVIVEK"
     gene            complement(9378..9803)
                     /locus_tag="PKZ49_06960"
     CDS             complement(9378..9803)
                     /locus_tag="PKZ49_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36340.1"
                     /translation="MNDLAAWEYFDNTWLFAGLVAILLSILLKYWKINTTSSINRSTE
                     QFILKSQKILLALAFVFLTGNLLIPTTKEANEKNEFNQPHQSNVTPSTPIGNTRNELL
                     SSPPAPDRIRSNRPTLTQPLDYNKNSIAEDRSEGIVNDY"
     gene            complement(9876..10913)
                     /gene="mltB"
                     /locus_tag="PKZ49_06965"
     CDS             complement(9876..10913)
                     /gene="mltB"
                     /locus_tag="PKZ49_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HND36341.1"
                     /translation="MRLPIPKNKLKKDIHRLPTYYMVILVLGVCMLLWGATAIGSPTR
                     PEIKTFIDEMVAEHGFNRPKLEAIFSKVQFQPSIINLISTPSSSVPWSQYRSRFVNLQ
                     RIKNGVSFWNQHANVLQRANKEYGIPEEIIVAIIGVETSYGASTGKHRVIDALSTLAF
                     DFPKRAEFFRSELVQYLLLAQEQNFDLLGIKGSYAGAIGIPQFMPGSYRRFAVDFDGD
                     GKVDLANSAADAIGSVANYLKEYGWEMGGPIVVQSSVKADRLQEILESGIELKHTIKQ
                     LQEASIIPLQSFENERLAALIELKDNENFQYWLGFKNFYVITRYNRSSFYAMSVHQLA
                     EAIRNARGSLR"
BASE COUNT         2974 a         2085 c         2453 g         3436 t
ORIGIN      
        1 tcgtcagtga tgtacatagc aatttctgtt ttgagtttct ccttgtttga caaattgtgg
       61 cttgaattag gttgaagtac gtgacgaggc ccctcagacc ctacccaatg tggtagggtt
      121 ttttttgtat tattatttta ctgattactg cgtcccatag cgctcacgat aggcctgaat
      181 ggcttgtaag ttttgtcgca tatttgtagc ttcttttaaa taatcgacaa tatcattgag
      241 atcaataatg ctgattaccg ggatatggtg caagttgttg acttcctgaa cggcagatag
      301 cttatttgaa ccttgctcta accgatcgag cgcaatgatt acaccacaag gcgttgctga
      361 ttcagcccgg atgagattga cggattctcc tattgaagtc cctgcagaaa taacatcatc
      421 agcaatgagt acgcgtccac tgagtggtgc cccaatcgtt atgccccctt caccatgatc
      481 ttttagctct tttcgattaa agcagaaagg gtaattgtaa ccaagctcag caagcgctat
      541 cgcgatggtg cttaccaatg gaataccttt gtaagctgga ccaaataaca tatcaaaggg
      601 aagttgggca gcgataattg ctttagcgta gaattggcct agttgacgca aagcgttacc
      661 attattgaat aagccggcat taaaaaagta gggtgataat cggcctgcct tggttttgaa
      721 ttcaccaaag cgaaggacat tacagccaat ggctagttcg ataaatgctt ttttgtaatc
      781 tgacatagtt taatttagta gcataaatga gaattataac gcttaacgta aatgggattc
      841 gtgctgcagc caaaaaaggg ctttttaatt ggttaccgtt gcaacaggct gatgtaatct
      901 gtgtgcagga gctgaaagca gggattgacg atatgagtcc tgaaatgaaa gcatttgacg
      961 gctatcaggg ctattttcac tgtgctgaaa agaaagggta cagtggtgtt ggggtttata
     1021 cacggaacaa accagatcag gtgattgaag gcgttggtat tccgggaatc gattcagaag
     1081 gtcgctattt gcgtgtcgat tttggctcgt taagcattgt ttcaatttat cttccttctg
     1141 gctccagtgg tgagcatcgc caggatgcta aattttattt tatggaacat tttttcccct
     1201 ttctggagga attttcaaag gagggaagag aaattattct gtgtggtgat tggaacattg
     1261 cccataaaga aatcgatttg aagaattggc gatccaatca aaaaaactct ggatttttac
     1321 cggaggaaag ggcgtggtta tcagcggtat ttgaagaact gaaatttgtt gatgtgttcc
     1381 gtcgcctaaa ttcagaatcc gagcagtata cctggtggtc aaaccggggc caggcatgga
     1441 ctaataatgt aggctggcgt atcgattatc aaattgcaac agcttcgatt gcggataaag
     1501 caacacaagt ttccatatat aagcaagaac gtttttcaga tcatgcgcca ctgatcatcg
     1561 attacgacta ccaattatga aaaatacttc gctaatcgca tggctagatg catgccggat
     1621 ttatcttcat ccgagagtga tgggaatgtt agcgctgggt ttttctgcgg gactgccttt
     1681 attgctgatt ctaggcacat tatcattttg gttaagagaa gcaggcattg atcgaaccac
     1741 gatcggacat ttaagttgga ttggcttggc ttacggcttt aaatggttgt ggtccccctt
     1801 ggtcgatcgt atgcctttgc ctatcttgac taagctgctc ggtcgtcgcc gtgcatggtt
     1861 gttattgtca cagattacga ttgtgatagc actgattggt atggcaagca cagatccgct
     1921 attgaaccta acgcacatgg ttttttttgc attagcggtt gcttttgcct ccgcaaccca
     1981 ggatatcgca cttgatgctt atcggatcga ggcggttgca atagagctac aaggtgcgat
     2041 ggcggcaact tatcaagcgg gctatcgatt ggctatgata ctttcttcag cgggtgttct
     2101 ttggattgcg gcttcgctcg atacaatgcc tacgggttat gattatcgtg cttggcaaat
     2161 cgcctatttt gtaatggcgg ccttcatggg aattggtgtg ctcacaacac tgattatcca
     2221 agagccagcg gtgaaaatca gtcagcttgc tgttgaaaac gaaaaacgag tagaagcgat
     2281 gattgctcgc tggaatttaa atcatcgact tgcccaatta ttactgtggt tctatggtgc
     2341 agtaatcgcg ccctttcggg attttattgt gcgtcaccgc tacaatgcat tcttaatctt
     2401 agcattgatc gcagtttatc ggatttctga tgtggtcatg ggggtgatga gcaatccttt
     2461 ttacgtcgat atgggctata ccaaagacga agtcgcgaca gtttcaaaag tttatggtgt
     2521 tctgatgacg attgcaggcg cagcggtggg gggggtgctc attatgcgaa tcgggattat
     2581 taaaacgctg tttattggtg caatactatc tgcagtaacc aatctattgt ttgtatggtt
     2641 gacggtaatc ggccatagtt tacccggatt aatttttacc atttcagctg ataatctttc
     2701 ggcaggaatt gcttcatcag cgtttatagc ttatctctca ggtctgacgc attctgctta
     2761 ttctgcgacg caatacgcat tgtttagctc agtgatgcta cttttaccta aatttatcgc
     2821 aggatttagt gggcagtttg tcgatgccta tggctatgcc tggtttttcc tcagtactgc
     2881 actactagga ttaccggttc tgatcttggt ttggcttgtt agtcgtatcg atttccattc
     2941 atcggaatag cccgagtgtc gtaatgcttg gcctttttct ctctttgaat tgagctaatc
     3001 tcataggatt ggtaaacaat aggtaatcta ccagaatgac tacttgatac gctaaagcct
     3061 ttgctgagta atatgattaa taatccggaa gttaataatt tttcatagat tgccgtcata
     3121 tgaatcatta tcgattttta ttgttaagtc tatgcaatcc aaaacgatca ctggattaaa
     3181 aaactggatt agttttttta ataaagcaga aatcccagtc ttgaaactaa cgggtaatga
     3241 gctgttttta ttgcttcagc aggaaaaaaa tcctagcgct cgcagtgttg caaacatcat
     3301 taaacgagat ccactgatga ctgtgaaatt acttcgctat ttttgtcagc ataagcacag
     3361 gagtctggaa cacgaagtga tgcaagctga gcagatcttg attatgcttg gggttgataa
     3421 ggcgatgaag aatgttttac cagagccgac ggttgaggaa ttgttgcgcg agcagaaagt
     3481 tgaagttctt attaacttac ttcatttagt tcatcgttcc catattgcct caaactatgc
     3541 tttcgaatgg gctgttcgtc tccatgatac gcattttgaa gaaatacgta ttgccgcatt
     3601 gctacatgat atttcagaat tattaatgtg gtgttttgcg cccacggaga tgctaagaat
     3661 tcgagaaatg caaaaaaaag ataaaacact acgaagttat cttgctcaaa aacaagtact
     3721 cggatttacg ctagccgatt tgcaatcaga attagttctg agatggtcat tacccgagct
     3781 gttgattacg cttatggatg accacaatgc gtcactacaa cgtgtgcgta atgtcgtatt
     3841 ggcagtaaac ctagcgcgcc attctgcaaa tggatgggat gatgcagcat taccggatga
     3901 ttatactgca attggtgaat tattaaggat gccagtacat gatgtgaaga taattgtcgg
     3961 cgcaaaaaat attgagccgg tcaattctca gcagtctgaa cattagctaa taattattag
     4021 attattcgat attctggact tgctcacgca tctgctcaat cagtacctta agttcaactg
     4081 aaatttttga tacttcaaca tcgagtgatt tcgaacccaa tgtattcgat tctcgattta
     4141 actcttgcat taagaaatcg agacgttttc cacttgcccc acctttaagt agggtttgct
     4201 cgacttcatc gagatgcgta ttgagtcgtg ataattcttc gtcaatatcg attttacttg
     4261 caaataaaac aagttcttgt cggatacgat cgtcattgca atcaatctgt gcctcttgca
     4321 agcgtaagat taatttttcc tgaaaattct taatgagtgt tggaatacgt ggtgataacg
     4381 ctgaagttaa ctgacgcatt tgggttagtc gatcaatcaa aatcgcttta agtttctcac
     4441 cttcacgtaa tcgtgttgag acgagttccg tcaacgttat ttgtagtaag ttgttacacg
     4501 cttcttgtaa atcagtggtt ggcgaagaat cactcactaa gatgccaggc catttaagaa
     4561 tttctgcaac actcaagctt cgcgcagcaa taaaatttga ttgaactgta ttatttagat
     4621 ccagtaattg acggattact tgggaattaa gctgttgggg aaggtttaaa tcgggatagg
     4681 attgaaaggt taggcggcaa tcaatttttc ctcgttttat ttttgtattc agcaattcac
     4741 gcatacccgc ttcgaagcta cggaattcat cgggtagttt gaattggata tcaagataac
     4801 gattattaac agaacgaagt tccatggaaa tcaggccact ggaaatttcc tgggtcgctg
     4861 tggcgtatcc tgtcatgcta gtgatcataa tgcgattatc ctgttgacat ttaataatgt
     4921 gtaagcttac ccctgttttc tctactcatg gacttgttcc cataattttt gtagacgctt
     4981 aactgaaaca gggtaaggtg tttttaattc ttgagcaaat aatgaaatcc tcaattcttc
     5041 gatttgccag cgaaattctt caagctttgg atctttgaat ccacgcttgc tgtgtttctc
     5101 atatctttgg cgatattgat tccataatac tgagatggca agcgcatgct ggttatcacg
     5161 tgctggattg ttagggtact tatctaaccg aacgagcatt ccttttaagt accgaagtaa
     5221 gtgtggaagt cttacccagt cagtttgctg caggaaatta ggataaatga gcgcctgaag
     5281 ctgattgttc aattcttgtt ttatttgagc atgatgcgga ttgacagtgg acagtttctg
     5341 tttgaatgcc tgatattcga gcgcaagatc atggataaat tgaataagtg tgtcagcgac
     5401 ggagtttaaa cgaattcggc ctttctgctt caactgggaa aactcatgtt cattccgtgg
     5461 taaaggttca tcgccaatca aggcacgatc atagattgcg gcaaacatat cttgcttgag
     5521 tgtttctgaa tcgatgagat ggcttaatac taacgaatca gacttggtgc ttggtaattt
     5581 cttttctaat agcttaatag ggtcttttaa ttccaaaatg agcagtcgtt tgacgccact
     5641 tcgcatatga ttgtgcgcta cctcgatagt atcaaataag cgaattgcaa catggtcaat
     5701 ctgatcaacg agcgctgggt atcctgttaa ttgtttttgt gctcgaataa aagtgacttc
     5761 ctcagggagt tggccaaagt tccattgcgt aatttgatca cgttctatac caagctgatt
     5821 tgctgcgtta ttttgcgaaa agatcagttg agcagtttgc ccaaattgat cttgtaattc
     5881 tattaaatca cggtttaccc caagctcttt cccggcatca tcgataattc gataattcat
     5941 ttgcaggtgt aatggcatta attttgtatc ccaagtatcg gatgagattt ttgtacctgt
     6001 tatgctaaaa atatactcag aaagcgcttc aagtaaggaa agcgaatgct tttgcttatc
     6061 acggtaatct aggaaatggg ttacatgctc agcaatagga atgagattgc gtcgaattgg
     6121 cttaggtaat gctttaaagc accaggtaat tttttcacga attaaaccgg gtactaaatc
     6181 ttgaaactga ttcgctttta gtttatttaa tgaatgaagt ggggcagtta ctgtaacgcc
     6241 atccatagcg tgacccggct caaaacgata ggtgagtggg agttgttgat caaaaatgga
     6301 gattgattca ggaaatagcg tttcaaaagc tgtatctgct ttatggcgca ttaaatattc
     6361 ccggctaaga taaagcaaat gggggttatc ctgctctact tgtttgcgcc agctctcaaa
     6421 tgagctgcca ttcaatatat tgtcgggaat tattttgtcg taaaaagtaa aaatatcttg
     6481 ttcttcgact aaaacatctt gacggcgggt tttgtgttcg agttcttcga tgtcttgaat
     6541 gagctggcga ttatgtttaa ggaattgagc attggtttcg tagtcgccaa gaaccaatgc
     6601 attgcgaata aaaatttccc gagcttcttt ggggttcatc ttcccatacg taacgctgcg
     6661 cttcggcaca atcataagtc catagagcgt aacgcgttca tataccataa cttgtgcagt
     6721 tttcttttcc caatggggat caaaaaaatg ttttttacaa agcttgcctg caattttttc
     6781 aagccatgtc acatcaatcg tagcagcaca tcgtgcatag agctttgtcg tttcaacgag
     6841 ctcagctgct acaatccact tggcttttgc ttttcttagc gtagagccag gataaataaa
     6901 gaattttatc cctctagcgc caagatactc cccttcattc tcacttttaa acccaatatt
     6961 gctaagtaaa ccggctagta aggcacgatg aatttcatca taatttgctg gaacttggtt
     7021 gggccttagt ccaaattcca gaactaacgt atggagttgg ccgtgaattt cacgccattc
     7081 ccgcattttg cgatacgaaa taaagtgctc gtgacattgt gtgattaatt ttttctttga
     7141 ttttttgtgt ttaagcaatt catcaaagaa attccaaagt ttcagaaatc caataaaatc
     7201 agagcgctcg tcatgaaagg ggagatgagc acgatctgct gcttcttgtt gatcaaaggg
     7261 tcgttcacgc ggatctgcca aacttaatac cgatgcaatg atcaatactt cgcttaagca
     7321 gttttcttgt ttggcggcaa gaatcatgcg tgcaattttg ggatcaattg gaaattttgc
     7381 aagttgccag ccaatggtcg tcagtttttg atgaatatct agggcgccta attcgctgag
     7441 cgattgatag ccatccgcaa tcatgcgagg taaaggcggc tgaatgaatg gaaagttctc
     7501 gatatcacca atcttgaggg atttcatgcg caggatgacc gaagccaatg atgtgcggag
     7561 aatctctggg tcggtgtata gtggacgtgt ttcaagctct tctttagcgt agagtcgaat
     7621 acaaatacca ctcatgacac ggccgcatcg tcccgcacgt tgattcgctg aagcttgtga
     7681 aattttttca acgagtaact gctctatttt attgcgatgg ttatagcgat taattcgtgc
     7741 aacaccagta tcaatgacgt agcgaatgcc tggaacggtt aacgaagttt cagcgacatt
     7801 ggttgcaagg atgatgcgac tgactgcgtt tgatttatga aatacacgtt gctgctcagc
     7861 ataagataat ctggcataga gggatagaat ttctagtttc ccaggatcac tgaaatgatt
     7921 aaaacgatgc ttacgaaggg tctcggacgt ttccttaatt tcacgttcac cgggtaagaa
     7981 aattaatata tcccctattc cagtgcgtag aagctcatcc gtagcgtcca aaatggattg
     8041 ctgcagatcg tattcttcct catcttgatt attatctttt gcgtggtagc gtatttcgac
     8101 tgggtaggtt ctgcctgaga cttcgattac gggcgcattg ttaaaatggt gcgagaagcg
     8161 ctcagcatca atggtggccg aagtgataat cagctttaag tctgctcgtt tcggtaataa
     8221 ttgcttgaga taacctaata aaaaatcaat gttaaggctg cgctcatgag cctcatcgat
     8281 gataatcgtg tcgtattttt gcagataagg gtcgctttgc gtttcagcaa gtaaaatacc
     8341 atcagtcatg agcttgatat aggtgtcttg attgatgtta tctgagaaac gaactttgta
     8401 acccacagcg tgatcgagcg ggctactcag ttctgttgcg atttgtattg cgacagatcg
     8461 ggcggcaatg cgacgaggct gtgtatgccc gattaaacct gagactcctc taccaagctc
     8521 taggcaaaat ttaggaagct gcgttgtttt gcctgatccc gtttcaccac taacaataat
     8581 gactgagtta gatcgaatcg cgtccaggat cttgtggcgc tgcttgcaga taggtaattc
     8641 atctgaatac ttgggtttgg gtaaattgtt actccttcgt gccagatttt cagcagatag
     8701 gtgctgggaa atactcattc gggctaacga acacttaccg atttaaaggt tgcagggggc
     8761 gaaggcaatt atttgctata aaagatcgca atgaaattat ttttcgacaa taacggtctc
     8821 tttctgatta ctcaaggttc cctgttcaat gatggctacg gttaatcgag agatcgcgac
     8881 acgtttgcct gtggtttctt cggtgatatc ggtttgccat acttgggtgc gtcgaccaac
     8941 atgaaggggg gtgcagatac caatcacata tccttttgtt acagcacgga tatgattggc
     9001 attgatttct aagccgactg cgcgatattt ttctgggtca atcgtcatcc atgcagaaac
     9061 gctgcctaat gtttcagaca agacacagct tgcgccaccg tgtaagattc caaaaggttg
     9121 tgtggttcgt tgatcaacag gcatacgcgc tttcagaaaa ttaggcccaa tttcagaaaa
     9181 atgaatgccg atatgaatgc ccatgttggc attgcgtagc ccttccaagt aatcaagggt
     9241 atagggttta aaccaaatat tatccattgt tatgtgtgct tctgaagaga aatgtaaaca
     9301 ctttccactg tcgatagcga ttaggaaagc agctatttta attttattta ggaagcgcta
     9361 ggaacgggta atcgatctta gtaatcatta acgattcctt cgctgcggtc ttctgcgatg
     9421 ctgtttttat tataatcgag cggttgcgtc aatgttgggc gatttgatcg tattcggtca
     9481 ggcgctgggg gcgaagatag tagttcattt ctagtgttac cgatgggggt agatggcgtt
     9541 acatttgatt ggtggggttg gttgaattca tttttttcat tggcttcttt tgttgttgga
     9601 atgagcaaat ttcccgttaa gaaaacaaaa gcgagggcca gtaaaatttt ctgactttta
     9661 agaataaatt gttcagtact gcggttaatt gagctcgtcg tattaatttt ccagtatttt
     9721 aataatatag aaagaaggat tgctacaaga cccgcaaata accaggtatt atcaaaatat
     9781 tcccaggctg ctaaatcatt cattgttcaa aagctagaat taatctggtt ctttggctta
     9841 ttactcatga tcaaggaaaa tcaatacgtt gttgcctatc ttaatgagcc gcgcgcattt
     9901 cgaattgctt cagctaattg atgcacggac attgcataaa acgagctgcg attatagcgc
     9961 gtgatgacat aaaaattctt aaaacctagc caatattgaa agttttcgtt atctttcagt
    10021 tcgatgagtg cagcgagccg ctcattctcg aacgattgta atgggataat actcgcttct
    10081 tgaagttgct tgatggtatg tttgagttcg attccactct caagtatttc ctgtaagcga
    10141 tcagctttta cactggattg aacgacaatt ggtcctccca tttcccatcc atattctttt
    10201 aaatagttag caacgctacc gattgcatcg gctgcactat ttgctagatc aactttacca
    10261 tcaccgtcaa agtcaacagc aaatcgtcta taacttcctg gcataaattg aggaatacca
    10321 attgcaccgg cgtaagaacc tttgatgccc agtaaatcaa aattttgttc ttgagcaagt
    10381 aataaatatt gaacgagttc actccgaaaa aactcagctc ttttaggaaa atcaaatgca
    10441 agtgtactga gtgcatcgat gacgcggtgt tttccagttg atgcaccata ggatgtttct
    10501 acaccgatga tggcaacgat tatttcttca gggataccgt attctttgtt tgcgcgctgg
    10561 agtacattgg cgtgctgatt ccagaaacta actccatttt taattcgttg cagattaaca
    10621 aagcgcgaac gatattgact ccaaggaact gaggatgagg gagtcgaaat cagattgata
    10681 attgaaggct ggaattgaac tttgctaaat atggcttcca gtttgggcct attaaatcca
    10741 tgctcagcga ccatttcatc gatgaaagtc ttaatttcag gacgagtggg agaccctatt
    10801 gcggtggctc cccagagcag catacaaacc ccaagtacca aaataaccat gtagtaggtt
    10861 gggagcctgt gaatgtcttt ttttaattta tttttaggta ttggtagcct cataatagac
    10921 tgatataaag ttgggaagct tgatttaa
//
LOCUS       DAOQKQ010000085        10921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_2835_length_10921_cov_6.008559, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000085 DAOQKQ010000000
VERSION     DAOQKQ010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 10921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10921
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2835_length_10921_cov_6.008559"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="PKZ49_06970"
     CDS             <1..898
                     /locus_tag="PKZ49_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36342.1"
                     /translation="MQQGMRREESIALLDTHAETPPINWWILGGGAAFTVISLSVGLG
                     GISGAEEIVFALSMAIVLFLIKQISGELDEEARNMLVGTAILIFVFRAIPGPGAGSTW
                     WMIDHLGFDQQFLATLSLIGSALTLAGMFIFRRFMAERSMAFIIGILTIAGTILSLPI
                     IGMYFGLHEWTAALTGGVVDARFIAVVDTALESPLGQIAMIPMLAWIANSAPEKLKAT
                     FFAVMASFTNLALSASQLCTKYINQIFIISREVIDASTGEVTIPADYSQLGLLLMSIT
                     AIGFVIPLLTIFILKQTRFRIA"
     gene            1021..1335
                     /locus_tag="PKZ49_06975"
     CDS             1021..1335
                     /locus_tag="PKZ49_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HND36343.1"
                     /translation="MQRFLQLLVIFFVFSSTVFAGVNINTASEAEFEALKGIGPAKAK
                     AIVEYRNQHGHFKSIDDLEKVSGVGPGTINQIRNELIVDEIAAAPDPEKMVVKKEELS
                     SK"
     gene            1480..2364
                     /gene="cysM"
                     /locus_tag="PKZ49_06980"
     CDS             1480..2364
                     /gene="cysM"
                     /locus_tag="PKZ49_06980"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HND36344.1"
                     /translation="MFKTIQDCVGNTPLVQLKRIPGKTSNILLAKLEGNNPAGSVKDR
                     PALSMIMRAQERGAIKPGDTLIEATSGNTGIALAMAAAMMNYQLILVMPENLSIERRQ
                     SMSAYGAKIILTPKEGGMEHARDIAEQLRDEGQGFILDQFANPDNPLAHYKGTAPEIW
                     HETSQSITHFVSSMGTTGTIMGCARYFSEANPDIKIIGVQPEEGSQIPGIRKWSENYL
                     PKIYDPTLINRILFVSQTEAETMTKRLAREEGIFTGISSGGALAAALKISDEVEDATI
                     VFIACDRGDRYLSTNVFK"
     gene            complement(3070..5310)
                     /gene="clpA"
                     /locus_tag="PKZ49_06985"
     CDS             complement(3070..5310)
                     /gene="clpA"
                     /locus_tag="PKZ49_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HND36345.1"
                     /translation="MIAQELEASLHMAFIQSRQKRHEFITVEHLLLALLDNPSAAEVL
                     VACAIDIEELRTLLHDHINQHTPIVGGSEDVDTQPTLGFQRVIQRAILHVQSSGKKEV
                     TGANVLAAIFGEKDSHAVYFLQQKGITRLDVTNYISHKICKVSQNNEQNNKAEEESEN
                     EQQSAAPSPLESYTINLNNLAMANRIDPLIGREKELERVIQTLCRRRKNNPLLVGEAG
                     VGKTAIAEGLAKRIVEGDVPEILSKHQVYALDIGALLAGTKYRGDFEQRFKTILKQLT
                     DNPKAILFIDEIHTLIGAGAASGGTLDASNLLKPILSSGRLKCIGATTYNEFRGIFEK
                     DHALSRRFQKIEVNEPSIDETVAILRGLKSHYEKHHGVKYSSIALTSAAELSARFIGD
                     RHLPDKAIDIIDEAGAAQRILPKSKQRKVIGKTEIEEIIAKIARIPSQSISSDDRSKL
                     KTLDRDLKSIVFGQDAAIDVLAIAMKLSRCGLGNPQKPIGSFLFSGPTGVGKTEVARQ
                     LAYIMGIPLHRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTETIIKHPHSVLLLDE
                     IEKAHTDIFNVLLQVMDHGTLTDNNGRKADFRNVIIIMTTNAGAESLVKSSIGFTETS
                     QTGNEMTEITRLFTPEFRNRLDSIISFAPLSKGIILRVADKFLMQLETQLQEKKVEVI
                     FTDDLRNYLAEKGFDPLMGARPMARLIQDVIRSALADELLFGKLTNGGKVTVTISKEG
                     KVELQF"
     gene            complement(5307..5621)
                     /gene="clpS"
                     /locus_tag="PKZ49_06990"
     CDS             complement(5307..5621)
                     /gene="clpS"
                     /locus_tag="PKZ49_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HND36346.1"
                     /translation="MTIRNNREESVLEDNKNKLKTPPLYKVLLLNDDFTPMDFVVMVL
                     QTFFFMSQEKAIHVMLKVHNEGAGICGVYPSDIAMTKVKQVNDFSRQNQHPLKCVMEK
                     NT"
     gene            5969..6172
                     /locus_tag="PKZ49_06995"
     CDS             5969..6172
                     /locus_tag="PKZ49_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HND36347.1"
                     /translation="MATGTVKWFNDSKGFGFITPDDGSEDLFAHFSAINMTGFKTLKE
                     GQKVSFEVTQGPKGKQASNIQAA"
     gene            complement(6293..7543)
                     /gene="icd"
                     /locus_tag="PKZ49_07000"
     CDS             complement(6293..7543)
                     /gene="icd"
                     /locus_tag="PKZ49_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HND36348.1"
                     /translation="MFQHIKIPAEGKKILVNSDQTLNVPDNPIIPYIEGDGTGVDITP
                     VMLKVVDAAVSSAYGGKRKIAWMEIYAGEKSTKVYGENVWLPDETLQAAKEYVVSIKG
                     PLTTPVSGGIRSINVALRQLLDLYVCLRPVRYFQGVPSPVKQPEKVDMVIFRENSEDI
                     YAGIEWEAESSAANKVIRFLIDEMQVKSIRFPDTSGIGIKPVSREGTERLVHKAIQYA
                     IDNKRSSVTLVHKGNIMKFTEGAFKNWGYHVAAKDFGAELYNGGPWMRLPQDKGGIII
                     KDVIADAFLQQILLRPEEYDVIATLNLNGDYISDALAAQVGGIGIAPGANLSDSIAIF
                     EATHGTAPKYAGKDQVNPGSIILSAEMMLRHMGWNEAADAILRAMEKTITSKIVTYDF
                     ARLMDGATEVSCSKFGSTLIANMS"
     gene            complement(7622..8110)
                     /locus_tag="PKZ49_07005"
     CDS             complement(7622..8110)
                     /locus_tag="PKZ49_07005"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HND36349.1"
                     /translation="MDNRFLWGFMAALVIWVSIFAVVYFFFASAQAPIVAMVNTDSVE
                     KEIVIPRSKDGHYYVRGKINGYPVDFMVDTGASVVSISYDLARSIHLPRGVPANFSTA
                     GGNMIGEIVSAIDIEVGNIRLSELTVSVGIQGNIALLGQNFLRRIDVIQSDDKMILRI
                     RN"
     gene            complement(8241..8984)
                     /locus_tag="PKZ49_07010"
     CDS             complement(8241..8984)
                     /locus_tag="PKZ49_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36350.1"
                     /translation="MENPLTKSYLSTHSVNAWKNTLTNLVRRSQLVVVSKLKRRRRYT
                     LVLHRLKRNLVLAGGLIGFMVGLSWITINYLAKESSYLSTEDQESYSIVVPPHVFIDS
                     IDTLEKWLRKELKLPYAKSHMIIRPASVLSGNKIAFTQLPESWEIKFLTHNSNFSVRY
                     WKGDTDVLVIKREASFWALLKNIHDDSTSNSAWVLLTDAVVGGVSSYVVINSLIWSRL
                     YYLRLLAVGFIGITLAISFGLVLNSFSLY"
     gene            complement(9439..>10921)
                     /locus_tag="PKZ49_07015"
     CDS             complement(9439..>10921)
                     /locus_tag="PKZ49_07015"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HND36351.1"
                     /translation="LPELDTCLLSLAQKGWIEYNEATISFKCSPVVQEVTRQKNPGLR
                     EDCTMLVNTLIEKLGYDGRHMTGSSYDEAHLYARYAEAVMKTFLMKPDDNLLALSERT
                     GNYHRITGNLERSLFFFEHLTQVCKTLLEGAPDDPALKNNLATSYSELGTTHKAMGNL
                     QQALTCFEQSNRLGKELFDTYPQDALCKGFLAISCQYLGDTHKAMGNLEQALTFFEQS
                     NQLEKALSDAAPQDVGFKHELAISCQHLGITHKALGNLKEALDFFEQYHRLEKELVDV
                     YPQNVEFKYNLAASYQFLGSTHTALGNLKEALDFSGQYHRLEKELADAYPQNVEFKDS
                     LAISCQHLGITHTALGNLKEALDFFEQYHQLEKELADVYPRNVGFKNGLALSCQCLGG
                     THQALGNLKEALDFFEQYHQLEKELADVYPRNVEFKNNLALSYQWLGWFYEDKLQDLN
                     QGQAYYRLSKSLLLELVSSFPGYVQFKQSLDWVTSRLPDEADE"
BASE COUNT         3224 a         2376 c         2060 g         3261 t
ORIGIN      
        1 aatgcaacaa ggaatgaggc gtgaagaatc gattgcccta ttagatacgc atgctgagac
       61 ccctccaatc aattggtgga ttctgggggg aggcgccgcc ttcacagtca tctcattaag
      121 tgtaggatta ggtggtatta gcggcgcaga agagattgtt tttgcattat caatggccat
      181 cgttcttttt ctaataaagc aaatctcggg cgaattagat gaagaagcgc gcaatatgct
      241 ggttggaacg gcaattctaa tctttgtatt tcgagccatc cctggaccgg gtgctggctc
      301 aacttggtgg atgattgacc accttggttt tgaccaacaa tttctggcaa cgctctcatt
      361 aatcggttcc gccttaacgc tcgcaggtat gtttatcttt cgacgcttta tggcagagcg
      421 atcgatggct tttattatcg gaatactgac aattgcaggc acaatccttt ctttacctat
      481 cattggaatg tactttggat tacatgaatg gactgccgcg ttgacagggg gtgtcgttga
      541 tgcacgcttt attgctgtcg tagacacagc gttagaatcg cctttagggc aaatcgctat
      601 gattcccatg ctagcttgga ttgccaattc agcccctgag aaactcaaag ctaccttttt
      661 cgccgttatg gcatctttta ctaatcttgc actatcggca tctcaattat gcacaaaata
      721 tatcaatcaa atattcatca tatccagaga agttattgat gccagtacag gtgaagtcac
      781 tattcctgct gattatagtc aattaggttt attactgatg tctattaccg ctattggttt
      841 tgtgattcct ttgcttacaa tttttatctt aaaacaaaca cgtttccgaa ttgcctaatc
      901 cttttatttg atttttcatc ctagcctaaa actccacaac gatctgccga tatattctgg
      961 ccttttgatt gcacgatttg tatttcactt ggcaatcttt tttaaacgat aggagatttt
     1021 atgcaaagat ttttacaact attagtaatc ttttttgtat tcagtagtac tgtttttgct
     1081 ggcgttaata tcaacacagc atctgaagcg gagtttgaag ctttaaaggg aattggacca
     1141 gctaaagcaa aggctattgt agagtatcga aatcaacatg gccatttcaa atctattgat
     1201 gatttagaga aagtaagtgg cgttggtccg ggcaccatta atcaaatccg caacgaatta
     1261 atcgttgatg aaattgccgc tgcacctgat cccgaaaaga tggttgtaaa aaaagaagag
     1321 ctgtcttcta agtaaataaa gctagcttca tcaaaaccct ctgaaatata atttcagagg
     1381 gtttttgctt ttttattaac cctatgcttt atcaagtctg taccctgtat gtaataattc
     1441 catttattca ttggtattta aaaaaatcca aactcaccta tgttcaaaac aattcaagat
     1501 tgtgttggca ataccccact cgttcaactt aaacgtatac cgggaaaaac cagcaatatt
     1561 cttttagcga agttagaagg taacaaccca gcaggatcag ttaaagatcg gccagcactt
     1621 tcaatgatca tgcgtgcaca agaacgtggc gctattaaac caggtgatac tttgattgaa
     1681 gcaaccagtg ggaataccgg cattgcatta gcgatggctg ctgctatgat gaattatcag
     1741 ttgattttag taatgccaga aaatctgagt attgaacgac gccaatcaat gagtgcatac
     1801 ggcgcgaaaa tcatcttaac tcctaaagaa gggggtatgg aacacgccag agatattgcc
     1861 gaacaactac gagatgaagg tcaaggattt attcttgacc aatttgctaa tcctgacaac
     1921 ccattggccc actacaaagg cacagcacct gaaatttggc atgaaaccag tcaatcgatt
     1981 acccattttg tcagcagtat gggcacaaca ggaacgatta tggggtgcgc tcgatatttt
     2041 tccgaagcta atccagacat taaaattatt ggtgtacaac ctgaagaagg atctcaaata
     2101 cctggtatca gaaaatggtc tgaaaattac cttcctaaaa tttacgaccc aacactcatt
     2161 aatcgtatcc tctttgtctc ccaaacagaa gctgaaacga tgacaaaacg cctagcacgc
     2221 gaagaaggaa tttttaccgg tatttcatca ggcggtgcac tcgctgctgc acttaaaatt
     2281 tcagatgaag tcgaagatgc tactatcgtt ttcatagctt gtgatcgagg tgatcgttat
     2341 ctatctacta acgtgtttaa ataattgtcc ttgtaaatac atccacaccc cagatataaa
     2401 gtgtaaatgg ttgtatgctt gcagatcaaa ttgactaata ttgtcttacc acagtacgaa
     2461 agtagtctat ttgcttaaac acattggtcg agcaaatttt ctgaataagt attgatttac
     2521 atgggcctaa gacgttggaa gatacggttc ttattgatac gacgctacaa aaaaagaaca
     2581 gtacctatct tactgacagc aaactggcga tcaaaatcat taatcgcctg aacaagatct
     2641 actcgctgcg tgtacaggag aaaagaccac aagcaatacg aatatggcag caaggcatcg
     2701 attgccagta ccgtacaaga tcatttaatc gtcagcgtga tcaatcacga ataaaacctg
     2761 catgacagtc atacgttacc ggaaatattt tatcatgttg cagcatcgcg cggcaaaagg
     2821 gaagtacttc ttgaaaagag ttattggtcg atcatatcaa tttattgata gtcgcctacg
     2881 cctggaattt aagagctatg gctactggcc actttttgac tgtttttcca aggaaacagc
     2941 agtaaatttc atcttcttcc aatgagaata ataaaaacca ttttccccaa gaaggggaag
     3001 atcaggccca atttttcaat ctttataaca cttgtttgaa tattcatacc aacattaatc
     3061 ttgtgtgcct tagaattgaa gttctacttt accttctttt gaaatggtaa ctgtcacttt
     3121 ccccccattc gtcaatttcc cgaagagtaa ttcatcagcc aaggcactgc gaataacatc
     3181 ttgaatcaaa cgagccatag gacgggcgcc cattaaagga tcaaatccct tttctgcgag
     3241 ataattgcgc aagtcatcag taaaaataac ttcaactttt ttctcttgca attgagtttc
     3301 aagctgcatt aaaaacttat ctgctacacg taaaataata cctttactta atggtgcaaa
     3361 tgaaataatt gaatctaatc gatttctaaa ttcaggtgta aataagcgcg taatctcagt
     3421 catttcatta ccggtctgcg aagtttccgt aaaaccaata cttgatttca cgagtgattc
     3481 agccccagca ttagtagtca taataatgat gacattacga aaatccgctt tccgcccatt
     3541 attatcggtt aatgtgccat gatccatcac ttgaagtaat acattaaaaa tatcagtatg
     3601 ggctttttct atttcatcaa gcagcaatac cgagtgggga tgcttgataa tcgtttcggt
     3661 caataaacca ccttgctcaa atccgacata acccggtggc gctccgatta gacgtgaaac
     3721 agcatgccgc tccatatact cggacatatc aaaacggtgc aatgggattc ccatgatata
     3781 ggcgagttga cgtgccactt cggtcttacc gacacccgta ggaccggaga aaagaaaaga
     3841 tccaattggt ttttgcggat ttcctagacc gcatctcgat aatttcatag caattgcaag
     3901 cacatcgatt gcggcatctt gaccaaatac gattgatttc aaatcacgat cgagcgtttt
     3961 gagtttacta cgatcatcgc ttgaaatgct ctgagaggga attcttgcaa tcttagcgat
     4021 gatttcttca atttcggttt tgccaatcac tttacgttgc ttagattttg gtaggatacg
     4081 ttgtgctgca cctgcctcat caataatatc aattgcttta tcaggcaagt gccgatcacc
     4141 aatgaatcgt gctgaaagct ctgctgctga tgttagtgca atcgacgaat atttcacccc
     4201 atgatgtttc tcataatgag attttaaacc ccgtaatatc gctaccgttt catcaatgct
     4261 cggctcgttg acctcaattt tctgaaagcg gcgtgatagc gcatgatctt tctcaaaaat
     4321 acctctaaat tcgttataag ttgttgcacc aatgcatttc aatctcccag aactcaatat
     4381 cggtttaagt aaattcgatg catctaacgt acctcccgaa gctgcgccag caccgatcag
     4441 tgtatgaatt tcatcaatga atagaatggc ttttggatta tcagttaatt gctttaggat
     4501 agttttaaac cgttgttcaa aatcaccgcg atatttagtg ccagcaagaa gcgctccgat
     4561 atccaatgca taaacttgat gtttagacaa aatctcaggt acatcccctt ctacgattct
     4621 cttggctaga ccctcagcaa tcgctgtttt accaacaccc gcttccccaa ccagtaacgg
     4681 gttatttttc cgacgccggc atagggtttg aatcactcgt tctaactctt tttccctacc
     4741 aattaacgga tcaatacggt tagccattgc caggttattc agattaatcg tataactttc
     4801 taatgggcta ggcgcagcag actgctgctc gttctcactt tcttcttcgg ctttattatt
     4861 ttgctcgtta ttctgcgaaa ccttgcagat tttatgggat atgtaattcg ttacatccag
     4921 tctcgtaata cccttttgtt gaagaaaata aactgcatgc gaatctttct cgccaaagat
     4981 tgcggctaaa acatttgctc ctgtaacttc tttctttcca gatgattgaa catgcaagat
     5041 tgcgcgctga attacccgtt ggaatccaag tgttggttgt gtatctacgt cttcgcttcc
     5101 ccccacaata ggggtatgtt gattaatatg atcatgtaaa agtgtgcgca attcctcaat
     5161 atcgattgca caagcaacta atacttcagc cgcacttgga ttatcgagta gcgcaagcag
     5221 taaatgttct actgtaataa attcatgtcg cttctggcgt gattggataa acgccatatg
     5281 gaggctagct tctaattctt gagcgatcat gtatttttct ccataacaca ctttaatgga
     5341 tgctgatttt gcctagaaaa atcatttact tgtttcactt tagtcattgc aatatcactc
     5401 ggataaacac cacaaattcc tgcgccctca ttatgaactt taagcataac gtgaatcgct
     5461 ttttcttggc tcataaaaaa gaaagtttga agtaccatta ctacaaaatc cattggtgta
     5521 aaatcatcat taagtaacag cactttatac agcggaggtg ttttgagctt atttttatta
     5581 tcctctagaa cactttcttc gcgattattt cttattgtca tcactgattc ctttttgcca
     5641 agctaatatc taaaaacttt agactacagg gatttaatca taagtcttca tcaacattag
     5701 cattctaatt atttttcttt ttacatattt gacgattacg acaaaatttt caagcgtatt
     5761 agctgcattc acttatttta ctgctactta aaaaaagtat aaaagtgata ttttcaaatg
     5821 ctaaacgctt gacttaagat taaaatttgc gtaaaactgc aactggcgtt tggcttcaag
     5881 ttccctgcag tattggttag agccgtttgc ggtcttgaaa ttcaaatagt tttagggttt
     5941 ccggcccagt tttttatagg aagtagagat ggcaacaggt acagtaaaat ggttcaatga
     6001 ttctaaaggg tttggtttta ttactcctga tgatggaagc gaggatttat tcgcccattt
     6061 ctcagcgatt aatatgaccg gttttaaaac tctgaaagaa ggccaaaagg tgagctttga
     6121 agtaactcag ggtcctaaag gaaaacaggc atcaaatatt caagctgctt aaggtagatt
     6181 gtttgatcta ctttgattag tttattttag ctataaagca acttgcccat tactataata
     6241 ctgttaatca ttattatgta atgggcaaaa cataaattct aagaacaata cactaagaca
     6301 tattagcgat aagtgtactt ccaaattttg aacaagaaac ctcagtagcg ccatccatta
     6361 accgagcaaa atcataggtg acaattttgc ttgttatggt tttctccatc gccctcaaga
     6421 ttgcgtcggc tgcttcattc cagcccatat gccttagcat catttcggcg gaaaggatga
     6481 ttgatccagg attgacttga tcttttccgg catatttagg tgctgttcca tgagttgctt
     6541 caaaaatagc aatcgaatca ctaagattcg cacctggtgc aatcccaata ccaccaacct
     6601 gtgctgctaa cgcatctgaa atataatcac catttagatt taaagtggca atgacgtcat
     6661 attcctctgg ccgcaataag atctgttgta agaaagcatc tgcaatcaca tctttgataa
     6721 tgataccccc tttatcctga ggtagtctca tccaaggccc gccattatag agctctgcac
     6781 cgaaatcttt agccgcaaca tgataacccc aatttttaaa tgccccttcg gtaaatttca
     6841 taatattgcc cttatgcacc agtgttaccg aagatctttt gttatcaatt gcatattgaa
     6901 tcgctttatg aactaacctt tctgtacctt ctcgcgagac tggtttaatg ccaatccccg
     6961 atgtatcagg aaaacgaatg cttttcacct gcatttcatc gataagaaaa cgaatcactt
     7021 tattcgccgc tgaagattca gcctcccact cgatgcccgc atagatatcc tcagagttct
     7081 ccctaaaaat caccatatca actttttcag gttgtttaac agggctgggc actccctgga
     7141 aataacgtac tgggcgtaag catacataca aatctagcaa ctgccttaat gcaacattaa
     7201 tagaacgaat ccctccactg actggcgtag ttaatggtcc cttaatagaa accacatact
     7261 ctttagctgc ttgtaatgtc tcatcaggta gccaaacatt ctctccatag actttggttg
     7321 atttctctcc cgcataaatt tccatccagg cgatttttct tttacctccg taggcgctcg
     7381 aaacggcagc atcaactact tttaacataa caggtgtgat gtcaacaccg gttccatcac
     7441 cttctatata gggaataata gggttatccg gtacattcaa cgtttggtca gaattaacaa
     7501 gaattttttt accttccgca ggtattttta tatgctgaaa catattagtc tccgatcatc
     7561 tcgctcgtgt tagattagta acatcatcaa ttcaattttt gttcaagcta aagctatttt
     7621 tctaatttct aattcgcaat atcatcttat catcagattg gatcacgtcg attcttctga
     7681 gaaaattctg cccaagcaat gcgatatttc cttgaatacc cacactaaca gttagttcgc
     7741 tgagtctaat attaccgact tcgatatcga ttgctgaaac aatctctccg atcatattac
     7801 cacccgcagt agaaaaattt gcgggaactc cgcgcggtaa atggatggaa cgagccaaat
     7861 catagctaat cgatacaacg ctagcacccg tgtcaaccat aaaatcaacg ggataaccat
     7921 tgattttacc tctaacgtaa tagtgaccat ctttggagcg aggaataacg atttcttttt
     7981 ctacagaatc cgtattgacc atcgccacta tcggcgcctg tgccgatgcg aagaaaaaat
     8041 atacgacagc aaagatactc acccaaatga cgagcgctgc cataaatccc cacaaaaatc
     8101 gattatccat aaaaattatc aagtcacaat tttaaaataa gaaaataatc gaatcattgc
     8161 agcaagaaat agcttggata ggttattgta caccttctac cctaacagtc acgaaagaac
     8221 ccagagtcaa taacgctgat ctaatatagc gagaaggaat ttaaaacgag accgaatgaa
     8281 atcgccaacg taataccaat aaaaccaact gcaagcaatc ggagataata taaacgactc
     8341 caaatcaaag aatttattac cacataagac gatacccccc ctacaacagc atcagtaaga
     8401 agcacccaag cactattgct cgtcgaatca tcatgaatat tttttaacaa tgcccagaaa
     8461 ctagcttctc gctttataac taacacatcg gtatcaccct tccaatagcg aacactaaag
     8521 ttactattat gtgtcaaaaa tttgatttcc cacgattcag gcaactgcgt gaatgcaatt
     8581 ttattgccgg acaatacgct agcaggtcta attatcatgt gcgatttagc ataaggcagc
     8641 tttaattcct tacgtaacca tttttctagt gtatcgattg agtcaatgaa aacatgtggg
     8701 ggaacaacaa tcgagtagga ttcctgatct tctgttgata aataactact ttctttagct
     8761 aaataattaa ttgttatcca agaaagcccc accataaaac caattaagcc ccctgctagc
     8821 accaagttgc gttttaatcg atgtaatacc agcgtataac gccttctgcg ctttaacttg
     8881 gaaacaacaa caagctgact ccgccgtact aaattagtta gcgtattctt ccaagcattt
     8941 acgctatgag tagaaagata agatttagtt aatggatttt ccatcgagtt aacaatgcgt
     9001 gaagcacacg ttgcgtaata aagtagtgac tactgagtcc aagcaattca tgatcgtgag
     9061 tagagcagat atgccttcat tactgaaata atgcataccc aaccagattt acagcacagt
     9121 tgaaataaaa gacttaatca aagagcagca caaccacacc ggattaaaag gatactgcga
     9181 agtgaaaaat atccttgcaa gaataattgt gctttttcac tggcttttat tgaaattgtt
     9241 aaatttctac tgagcgcaaa agcaaataga acgacatacg aaggtctgtg aaactagatg
     9301 gagattaagt gattcttacc gtaagcctcc gattccgtca ggctacccaa attcttctcc
     9361 ttagagcttc aatagcttcc ggtgcaaacg acagcaaatc atcgatgcga ctgtttagcc
     9421 aagtagagag tagtcacttt attcatcagc ttcatcgggc aatctactgg tcacccaatc
     9481 caggctttgt ttgaactgca cataacccgg aaaactgctg actaattcca gtaacaagct
     9541 ctttgatagg cggtaatacg cttgcccctg attcaaatcc tgaagcttat cctcataaaa
     9601 ccaacccagc cattgatagg atagcgccag gttatttttg aactccacat ttcgcggata
     9661 gacgtcagcc agttcttttt ccaattggtg gtattgttca aagaaatcca atgcctcctt
     9721 gagattgcct agtgcttggt gcgtgccacc aaggcattgg catgaaagtg ccaagccgtt
     9781 tttgaatccc acatttcgcg gatagacgtc agccagttct ttttccaatt ggtggtactg
     9841 ttcaaagaaa tccaacgcct ccttgagatt gcctagtgcc gtgtgcgtaa tgccaaggtg
     9901 ttggcacgaa atcgccaagc tgtctttgaa ctcaacattt tgcggatagg cgtcagccag
     9961 ttctttttct aatcggtggt actgtccaga aaaatccaat gcttccttga gattgcctag
    10021 tgccgtgtgc gtgctgccaa ggaattgata tgaagccgct aagttgtatt tgaactcaac
    10081 attttgcgga taaacgtcaa ccagttcttt ttctaatcgg tggtactgtt caaagaaatc
    10141 caacgcctcc ttgagattgc ctagtgcctt gtgcgtaatg ccaaggtgtt ggcacgaaat
    10201 cgccaactcg tgtttgaatc ccacatcttg cggagcggcg tcagacagtg ctttttctaa
    10261 ttggttggac tgttcaaaga aagtcagcgc ctgctctaga ttgcccatgg ccttgtgcgt
    10321 gtcgccaagg tattggcacg aaatcgccag gaagcctttg cataacgcat cttgcggata
    10381 ggtgtcaaat aattcttttc ccaatcggtt ggattgttca aagcaagtca gcgcctgctg
    10441 gagattgccc atggccttgt gcgtcgtgcc aagttctgag tacgaagtcg ccaagttgtt
    10501 ttttaacgct gggtcatcag gggcaccctc aagcagtgtc ttgcagactt gagtaaggtg
    10561 ttcaaaaaag aacagactgc gctcaaggtt gccagtgatg cgatgatagt taccggtgcg
    10621 ctcgctcaaa gcgagcagat tatcatccgg tttcatcaag aaagttttca tcactgcttc
    10681 ggcatagcga gcgtacaagt gcgcttcgtc ataggaagag cccgtcatat gccggccatc
    10741 ataacccaat ttttcaatca acgtgttcac cagcatcgtg cagtcctcgc gcaagccggg
    10801 atttttctgc cgggtcacct cttgtaccac ggggctgcat ttaaaggaaa tggttgcttc
    10861 attgtattca atccaccctt tctgagcgag agaaagcagg caggtatcga gttcaggcaa
    10921 g
//
LOCUS       DAOQKQ010000086        10911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_2839_length_10911_cov_5.634672, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000086 DAOQKQ010000000
VERSION     DAOQKQ010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 10911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10911
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2839_length_10911_cov_5.634672"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..488
                     /locus_tag="PKZ49_07020"
     CDS             180..488
                     /locus_tag="PKZ49_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HND36352.1"
                     /translation="MSYYQKHIFFCVNQRENSERCCNDHHAQVMRDYAKDRIKTLKLS
                     GKGKIRVNNAGCLDRCNEGPVIVIYPDNIWYTYVDQEDIDEIIEEHLINDRIVDRLKI
                     "
     gene            521..1147
                     /locus_tag="PKZ49_07025"
     CDS             521..1147
                     /locus_tag="PKZ49_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HND36353.1"
                     /translation="MKNAVEQKLFVIGPAGKIETIITFPIHKPSGIAIIAHPHPLYQG
                     NMDNKVVYITNKALIEKGFITAKFNFRGVGASEGKYAEGVGEISDVLAVTRFMQENYG
                     ATLNHALLVLAGFSFGGAIQAFAAQQLNPHKLILIAPSVARMNTPQVADHAGATLIIH
                     GDHDDIVPLQSVIDWANPQGIAVTVIPDAAHFFHGKLTILKQTIMNFC"
     gene            complement(1167..2069)
                     /locus_tag="PKZ49_07030"
     CDS             complement(1167..2069)
                     /locus_tag="PKZ49_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HND36354.1"
                     /translation="MLHVTLRQLKVFESVARHLSFSKAAEELFLTQPAVSLQIKQLEE
                     VVSLPLFEQIGKRIHLTEAGQELYQYSRAIAQQLSDMELALDELKGLERGKLNIAVVS
                     TANYFAPNLLAKFCQRYKGVTVSLHVSNRETVLKHLADNLTDLAIMGQPPDGIDITAE
                     SFMENPLVIVAPPQHPLCSQQNIPVKRLEEEVFLVRELGSGTRNAMERFFVEQGIRIN
                     KGMEADTTEAIKQAVQAGMGLGVMSLHTIQLELETHRLKILSVEGFPIIRHWYIVHRK
                     HKRFSNVAKTFKAFLLIEAPALMS"
     gene            2270..3691
                     /locus_tag="PKZ49_07035"
     CDS             2270..3691
                     /locus_tag="PKZ49_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="form I ribulose bisphosphate carboxylase large
                     subunit"
                     /protein_id="HND36355.1"
                     /translation="MAVKTYNAGVKEYRQTYWMPEYTPLDTDILACFKITPQPGVDRE
                     EAAAAVAAESSTGTWTTVWTDLLTDLDYYKGRAYRIEDVPGDDTCFYAFIAYPIDLFE
                     EGSVVNVFTSLVGNVFGFKAIRGLRLEDVRFPIAYVKTCGGPPHGIQVERDMLNKYGR
                     ALLGCTIKPKLGLSAKNYGRAVYECLRGGLDLTKDDENVNSQPFMRWRQRFDFVMEAI
                     HKAERETGERKGHYLNVTAPTPEEMYKRAEYAKEIGAPIIMHDYITGGFCANTGLANW
                     CRDNGILLHIHRAMHAVIDRNPHHGIHFRVLAKILRLSGGDHLHSGTVVGKLEGDRGA
                     TLGWIDLMRESFIKEDRSRGIMFDQDWGSMPGVFPVASGGIHVWHMPALVAIFGDDAC
                     LQFGGGTLGHPWGNAAGAAANRVALEACVEARNQGIQIEKEGKAILTNAAKHSPELKI
                     AMETWKEIKFEFDTVDKLDVAHK"
     gene            3757..4113
                     /locus_tag="PKZ49_07040"
     CDS             3757..4113
                     /locus_tag="PKZ49_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose bisphosphate carboxylase small subunit"
                     /protein_id="HND36356.1"
                     /translation="MSEMIDYKSRLSDPASRKFETFSYLPAMDKEQIKKQVQYIVSKG
                     WNPAVEHTEPEYLMSNYWYMWKLPMFGETDVDRILKETEACHKANPNNHIRLVGYDNF
                     AQSQGTSMVIYRGKTV"
     gene            4232..5035
                     /locus_tag="PKZ49_07045"
     CDS             4232..5035
                     /locus_tag="PKZ49_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HND36357.1"
                     /translation="MSEAIKQYLIKDQPYYHPVADEVALYEAAYSVRMPMMLKGPTGC
                     GKTRFVEYMAWKLKKPLITVACNEDMTASDLVGRFLLDAQGTRWQDGPLTTAARHGAI
                     CYLDEVVEARQDTTVVIHPLTDNRRILPLEKKGELIHAHTDFQLVISYNPGYQSLMKD
                     LKQSTKQRFGALDFNYPDHDSETEIVAHETGIDKKIADKLVSIAERARNLKGHGLDEG
                     ISTRMLIYAGSLIAKNVDAHAACRVALVRPITDDPDMRDALDAAVSTFF"
     gene            5072..7399
                     /locus_tag="PKZ49_07050"
     CDS             5072..7399
                     /locus_tag="PKZ49_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation protein NorD"
                     /protein_id="HND36358.1"
                     /translation="MSINLSDYTELLESLPPETMALLENTWHDATRVLSPRGLDNYIK
                     GASALQGLGKGRSLVEAWIDEAPQVAKEIGEDVIGDLATTLLMLASKTSGAVIELIMS
                     TSTTAAKRLGDADLFRNYLQFLNTLLAQAPRGVRPMLDKLDVLFGQLTLGGLRRWALW
                     GAHAHRTNYEEQINYFSLSSKESLAVLQRERKGTLFVDIQRRINIYLRALWARDFFMR
                     PTSGDFETRQGYRPYIENYLIHLPDAYDAHGEIAASEVYRAAAAHAAAHLVETKYPIS
                     AESLNPLQMAVISVIEDARVEALSIKRFPGLRQIWAKLHDANPDMATTIGDYLNRLAR
                     ALLDPDYQDPDEWIVEGRLLFAQAQDRLSENQLSWEIGVQLAHKIMGKKIPFNPRTDV
                     LTAPYLDDNRYFWEFEEFNFERSQTAGYEEPKQIRKYVNVMELANEIDVETAGDDAQE
                     IWVVGTEFFPYEDMGVSFNEMEGKEPVSPPFHYAEWDYQIQLERPTWVTVQEKRAKAG
                     DIVLVDSIATQYKREISRMKFLLDAMQPQGVQRIRKLEDGDEIDINAAISSTIDIRMG
                     QQPDPRIMMRSVRKIRDISVLVLLDLSESTNETVSGQEFSVLDLTRQATVLLADAINK
                     IGDPFAIHGFCSDGRHDVEYFRYKDFNQPYDEQPKAKLAGMTGQLSTRMGAAIRHASH
                     HLKQQRSSKKLLLIITDGEPADVDVRDPQYLRYDTKKAVEEAGRSGVITFCMSLDPRA
                     DQYVSRIFGAKNYMVVDHVERLPEKLPLLYAGLTR"
     gene            7441..7743
                     /locus_tag="PKZ49_07055"
     CDS             7441..7743
                     /locus_tag="PKZ49_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36359.1"
                     /translation="MSQQVYAGIDLDENGGLTHLGRIVLDAWVFGILPETETCTGWSH
                     AQMQNLYEEVYAAWEPFAHLPSRLPPELSAKHEKLYGQAITIAKRKGWSAELGEDD"
     gene            complement(7789..8196)
                     /locus_tag="PKZ49_07060"
     CDS             complement(7789..8196)
                     /locus_tag="PKZ49_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36360.1"
                     /translation="MDITCYRSQEIKREVRLLPATTYNLACQLITRCTTDHLFVPIRA
                     MQYLAILDKKEFVFIDGERKCWVDIAWQNFQTQNRTSLDQPVAYEVVYYRENQEELML
                     RLQKEFPSALQILANKATIKSPAQIIPFPAINT"
     gene            8233..10032
                     /locus_tag="PKZ49_07065"
     CDS             8233..10032
                     /locus_tag="PKZ49_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HND36361.1"
                     /translation="MRATRLNKPPANRNDWKTIRSLLPYLWEFKGRVLIALMLLLLAK
                     LANVSVPLILKEIIDALDPAQTIVTLPLFLLIAYGVLRFCSTLFGELRDAIFAKVTQR
                     AIRRVAIRVFSHLHSLSLRFHLTRQTGGVSRDIERGSRGISFLLNFMLFNILPTLLEI
                     SLVMVILISKYDIWFFIITFITLLSYIALTVFVTEWRMIFRRTMNDMDSKANTRAIDS
                     LLNYETVKYFNNEAWETQRYDEHLQQWEKAAVRNQTSLATLNTGQSAIIAIGVTLLMM
                     LAASKVVSGEMTLGDLVLVNAFLLQLYMPLHFLGFVYREIKHSLADMERMFTLLAENE
                     EIQDQPNAIALNISHASITFKNVNFSYESNRQILFDVNFSIPSGSTLAVVGHSGSGKS
                     TLARLLFRFYDTSSGAILINGHDIKQITQKSLRSMIGIVPQDTVLFNESIYYNIAYGR
                     PDASEAEILAAAKSAHIHDFVQSLPNQYDSQVGERGLKLSGGEKQRIAIARAILKNPL
                     ILIFDEATSALDSRTEKNIQHELKQLSKNRTTLIIAHRLSTVIDADQILVMDKGRIVE
                     QGNHQQLLATNGIYAQLWEIQQREEDTTGSITI"
     gene            10235..>10911
                     /locus_tag="PKZ49_07070"
     CDS             10235..>10911
                     /locus_tag="PKZ49_07070"
                     /inference="COORDINATES: protein motif:HMM:NF037256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-containing carbonic anhydrase"
                     /protein_id="HND36362.1"
                     /translation="MLDSIKKMLQQLMGSSSEDSNQATDITFYTLGKFNLGVGKIGPD
                     NRTDIDQSVLKEMYRRVAAGEFNIPVSGTLPEICVDGRTNRQGSRINAPSAAGGSLTM
                     VYGRDLGDSGALNDKTEMQLTIETMDTLKGKGYTTAVHGDDHSNCGCGACAHAQKVYQ
                     HITDKIDPIANLTGNYGIVISDTEKASIMGKAKNRLNHPSFFAEDRSAIIQACQDHGA
                     NYEELVDA"
BASE COUNT         3185 a         2385 c         2320 g         3021 t
ORIGIN      
        1 aaagggcaca agtactggag gatcgactca ccgacctagt tacacaagaa aaaaggcaaa
       61 gccgcttatt agctttgctc gccacattac ttgctgcact gttactggta caaatatact
      121 tataagttat ctactttagc aatctagaac gattcttttg cctaattttt atcacgctaa
      181 tgagttatta tcaaaaacat atcttttttt gcgtcaatca acgtgaaaat agtgagcgtt
      241 gttgcaacga tcatcatgca caagtcatgc gtgattatgc gaaagaccgt ataaaaacgc
      301 ttaaactaag tggaaaaggt aaaattcgtg tcaataacgc ggggtgtctt gatcgctgta
      361 atgaaggtcc tgtcatcgtc atctaccctg ataacatctg gtatacctat gtggatcaag
      421 aagatattga cgaaatcatt gaagaacatt taatcaatga ccgtatcgtc gatcgcctca
      481 aaatttagtt tgcaaaaaag ccacttgatc gcaagccaat ttgaaaaacg cagttgagca
      541 aaaattattt gttatcggcc cagccggcaa gatagaaacg atcattacgt ttccaattca
      601 taaacctagc ggtatcgcca ttatcgcaca cccacatccc ctgtatcagg gaaatatgga
      661 taataaggtg gtttacatca ccaacaaagc gctgatcgag aaaggattca tcactgctaa
      721 atttaatttt cgaggcgttg gtgcaagtga aggaaagtat gcggaaggag tgggggaaat
      781 tagcgatgta ttagcagtga cccgattcat gcaagaaaat tacggggcaa cactcaatca
      841 cgctttatta gtcttagcag gcttctcatt tggtggtgcg attcaagcct ttgccgcaca
      901 gcagctcaat ccgcacaaac tgattctaat cgcaccttct gttgcacgca tgaatacacc
      961 acaagtggct gatcatgccg gagcaacctt aatcatccat ggcgatcacg atgatatcgt
     1021 accgctgcaa tccgttatcg attgggcgaa cccccaaggc attgcagtca ccgtcattcc
     1081 agatgcagca catttttttc acggaaaact caccattctt aaacaaacca tcatgaattt
     1141 ctgttaaatc taaataatag tttccatcaa ctcatcaatg cgggagcttc aatcagcaaa
     1201 aacgctttaa atgtttttgc cacgttagaa aaccgcttgt gtttacggtg cacaatatac
     1261 cagtgacgaa tgatgggaaa tccctcaaca ctcaatattt tgaggcgatg agtttcaagc
     1321 tctaactgta tagtatgcaa ggacattacc cccagaccca taccagcttg aactgcttgt
     1381 ttgatggctt cagtggtatc agcttccatg cctttattaa ttctaatgcc ttgctcaaca
     1441 aaaaaacgct ccattgcatt gcgtgttccc gatcccaact ctctaacaag aaaaacctct
     1501 tcctcaagcc gtttaaccgg gatgttttgt tgactacata aaggatgctg tggcggcgcc
     1561 acgatgacga gcgggttttc catgaaggac tcggcggtaa tatcgatccc atccgggggt
     1621 tgccccataa tggccagatc ggttagatta tcagccaaat gcttaagtac tgtttcccgg
     1681 ttagatacgt gcaaactaac cgttacccct ttataacgct gacaaaattt tgctaacaag
     1741 tttggcgcaa aataattagc tgtactcacc accgcgatat ttaattttcc gcgctccaat
     1801 cctttcaatt catctagcgc caattccata tctgacaact gttgagcaat cgcacggcta
     1861 tattgatata gttcttgacc tgcttcagtc aaatggatgc gtttcccaat ttgctcaaat
     1921 agtggtagcg acacaacttc ttccagttgc ttgatttgca atgaaacggc aggctgcgtt
     1981 aaaaagagct cctctgcggc cttagagaaa ctcagatgac gtgcaaccga ttcgaatact
     2041 tttaattgac gcaaggtaac atgcaacata gactaaatcc tgagataaga tcggtttatg
     2101 tataagtaaa atcttatcat atccatcaga attattaatt tgttcttatg cttatctctc
     2161 gctatagtta tccctgaatt gtatgtaatt caacattaac aacgtttcac cctatacctt
     2221 ctcaagttct ttgtagcttt ggcaggattc tcactaagga gactttacga tggcagtaaa
     2281 aacctacaac gctggtgtta aagagtaccg gcaaacctat tggatgcctg aatatacccc
     2341 gcttgatacc gatattctgg catgtttcaa aattacccca caacctggtg ttgatcgtga
     2401 agaagccgct gcagcagttg cagcagagtc ttctactgga acatggacca ccgtttggac
     2461 tgatttattg accgatctcg attactacaa aggtcgtgct tatcgtattg aagatgtacc
     2521 cggtgatgat acttgcttct atgcatttat tgcctatccg atcgatttgt tcgaagaagg
     2581 ctccgttgtc aacgtgttta cctccctcgt tggtaatgtt ttcggcttca aagcaatccg
     2641 tggcttacgc ttggaagacg tgcgcttccc tatcgcttat gtgaaaacct gtggtggccc
     2701 tccgcatggc attcaagttg aaagagatat gctcaataag tatggtcggg cattgcttgg
     2761 ctgtaccatt aaacccaaac tgggtctctc ggctaaaaac tatggtcgtg ctgtgtatga
     2821 gtgcttacga ggtggtcttg atctcactaa agatgatgaa aacgtcaata gccaaccctt
     2881 catgcgctgg cgtcaacgtt ttgactttgt gatggaagca attcacaaag cagaacgtga
     2941 aacaggggaa cggaagggcc attatctaaa tgtaactgca ccaaccccag aagagatgta
     3001 caaacgtgcc gaatacgcaa aagaaatcgg tgcaccgatc attatgcatg actacatcac
     3061 aggcggtttc tgtgccaata cggggctagc aaactggtgt cgtgataacg gtatcctact
     3121 ccatatccat cgtgcaatgc acgcggtgat cgaccgcaat ccgcaccatg gtatccattt
     3181 ccgcgtactg gcgaagattc tacgtctatc tggcggtgat catctgcact ccggtaccgt
     3241 agtgggcaaa ttagaaggtg atcgcggtgc aaccctcggt tggattgact tgatgcgcga
     3301 aagcttcatt aaggaagatc gcagtcgtgg cattatgttt gatcaagatt ggggttcaat
     3361 gccaggcgta ttcccagttg catcaggtgg gattcatgta tggcacatgc ctgcattagt
     3421 tgcaattttt ggtgatgatg catgcttaca attcggtggt ggtacattag gccatccttg
     3481 gggtaatgca gcgggtgcgg cggctaaccg tgttgcgttg gaagcttgtg ttgaagcacg
     3541 taaccaaggt atacaaattg aaaaagaagg aaaagctatc cttaccaatg ctgctaaaca
     3601 tagccctgaa cttaaaatcg cgatggaaac atggaaagaa attaagttcg aatttgatac
     3661 cgttgacaag ttagacgtcg ctcataagta acattgattg attcctctga actttaaaat
     3721 tcagaggaat accaaaccat accaggagtt ttaataatgt cagaaatgat tgattacaaa
     3781 agtcgattaa gcgatcctgc tagtcgcaaa tttgaaacct tctcctatct gcctgcaatg
     3841 gacaaggaac aaattaagaa acaagttcaa tatatcgtga gcaaaggctg gaatcctgca
     3901 gttgaacata ccgaacctga gtatttgatg agtaattatt ggtatatgtg gaaactgcct
     3961 atgtttggtg aaaccgatgt tgatcggatt ctcaaagaaa ctgaagcttg ccataaagcc
     4021 aatccaaaca atcatattcg tctcgttggg tacgataatt ttgcgcaaag ccaaggaaca
     4081 tccatggtga tttatcgcgg aaaaacagtt taatacgcag cacgggatta tccgtcttac
     4141 aacccccgct tgctgcaaag caacgggggt ttttatttcg tttcaaatga tttagtacga
     4201 ttttaccaag cgttatccgt tggagagaaa aatgagtgaa gccataaagc aatatttaat
     4261 caaagatcaa ccttactatc accccgttgc agacgaagtg gccctctatg aagctgccta
     4321 ctcagttagg atgccgatga tgctcaaggg accaacgggt tgtggtaaaa cccgattcgt
     4381 agaatatatg gcatggaagc tgaaaaaacc gctgattacc gttgcttgca atgaggacat
     4441 gaccgcttcc gatttagttg ggagattctt actcgatgca caaggtacgc gttggcaaga
     4501 tgggccgcta acgacagcag cacgtcatgg cgccatctgt tatttggatg aagtcgttga
     4561 ggccagacaa gacacaaccg tcgtcatcca cccgctgaca gacaatcgcc gtattttacc
     4621 gctggaaaaa aagggggagt tgattcatgc gcacactgat ttccagcttg tcatctctta
     4681 taaccctggc tatcaaagcc tcatgaaaga tttaaaacaa tcgaccaaac aacgctttgg
     4741 tgcgttggac tttaactacc ccgatcatga ttcagaaacc gagattgttg cccatgaaac
     4801 cggtattgat aaaaaaatag cggacaaact cgtttccatt gcagaacggg cacgtaatct
     4861 aaaaggtcac ggattagatg aaggcatatc aactcgtatg ctcatctacg caggaagtct
     4921 tattgcaaaa aatgtagatg cgcatgctgc gtgtcgagtc gccctagtac ggcctatcac
     4981 agatgatccc gatatgcgtg atgcgctcga tgctgcggtt tctactttct tctaaattgt
     5041 tttttatcga taacaatcat cgttaaatca aatgagcatt aaccttagtg attacacaga
     5101 actgctagag tcgctaccgc cggaaaccat ggcgttattg gaaaatactt ggcacgatgc
     5161 aacccgtgta ctctcgccgc gagggcttga taattacatt aaaggtgcat cagcgttgca
     5221 aggattgggc aaagggcgct cccttgtcga agcctggatt gacgaagcac cacaagttgc
     5281 taaagaaatt ggcgaagatg tcatcggtga tttagcaact acgctactca tgctcgcttc
     5341 taagacatcg ggcgccgtta tcgagttgat catgtcaaca tcgacgacag ccgccaaacg
     5401 attaggtgat gcggatttat ttcgtaacta cttgcaattc ctcaatacac ttttggcaca
     5461 ggcgccacgt ggtgttcgtc caatgctcga caaattggac gtcctatttg gtcaactcac
     5521 actaggcggt ctgagacgtt gggccttgtg gggagctcat gcccatcgga ctaactacga
     5581 agagcaaatc aattatttca gcttgagttc aaaagaatcc ctcgctgttc ttcaaagaga
     5641 gcggaaaggg actttatttg ttgacattca acgtcgaatt aatatttatt tacgtgcatt
     5701 gtgggcacgt gattttttta tgcgccccac ttctggggat tttgaaacgc gccaaggtta
     5761 tcgaccttat atcgagaatt atttgattca tttgcccgat gcttatgatg cgcatggcga
     5821 aattgctgca agtgaagtct atcgtgcagc cgcagcccat gctgctgctc atttggtcga
     5881 gaccaaatac ccaatttctg ctgaaagcct taatccatta caaatggctg ttatttcggt
     5941 tatcgaagat gcgcgcgtcg aggcactctc gattaagcgt tttcctgggc tgcgtcagat
     6001 atgggctaaa ctccatgatg caaaccctga tatggcaaca acgattgggg attatctgaa
     6061 tcgcttagca cgagcactgc ttgatcctga ttaccaagat cctgatgagt ggatcgtcga
     6121 aggtcgacta ctctttgcgc aagcgcaaga tcgcctcagc gaaaatcaac tttcctggga
     6181 aattggtgtg cagcttgcgc ataaaattat gggtaaaaag attccattta accctcgaac
     6241 cgatgttctg acagcaccct acttagacga taatcgctat ttctgggaat ttgaagaatt
     6301 caattttgaa cgctcgcaaa cagctggata cgaagagccc aaacaaattc gtaaatacgt
     6361 taatgtgatg gagcttgcca acgaaatcga tgttgaaaca gcgggtgacg atgcccaaga
     6421 aatttgggtt gtcggcactg aattctttcc ctatgaagat atgggtgtct ctttcaatga
     6481 aatggaaggt aaagagcccg tttcaccgcc gtttcattat gctgagtggg attaccaaat
     6541 tcagcttgag cgccccactt gggtcaccgt acaggagaaa cgcgcaaaag caggggatat
     6601 tgtgctcgtc gatagcattg caacacaata caaacgtgaa atttctcgca tgaaattttt
     6661 attggacgca atgcaaccac aaggggtgca acgcattcgg aaactcgaag atggggatga
     6721 aatcgacatt aatgctgcga tttcatcaac tatcgatatc cgcatggggc aacagccaga
     6781 tccgcgcatt atgatgcgct cggttcgtaa gatacgcgat atatctgtgc ttgtcttgct
     6841 tgatttatcc gaatcaacca atgaaaccgt ttcaggccag gaattttcgg tgctcgatct
     6901 gacacgacaa gcaaccgtgc tgttagctga tgcaattaat aaaattggtg atccctttgc
     6961 tatccatgga ttttgctcag atggtcggca cgatgtggaa tattttcgct acaaagattt
     7021 caatcaaccc tacgacgagc agcctaaagc caaattggcc ggcatgacag gccaactatc
     7081 gactagaatg ggagccgcga tccgtcatgc gagccaccac ctaaagcaac aacgctccag
     7141 taaaaaatta ctactgatca ttactgatgg agaacctgct gatgtcgatg ttagagatcc
     7201 ccaatatttg cgctacgata ccaaaaaagc agttgaagaa gcaggcagat cgggtgtcat
     7261 tactttctgt atgagtttgg atcctcgggc cgatcagtac gtttcgcgaa tttttggcgc
     7321 taaaaattat atggtcgttg atcatgttga gcggcttcca gaaaaattac ctttgctgta
     7381 tgccgggctt acccgataat tagtcacact acgaaattaa accttggaat cattgcgagt
     7441 atgtcacaac aagtctatgc aggaattgat ttagatgaaa atggaggact tacgcattta
     7501 gggcgtattg tcttagacgc ctgggttttt ggtattctac ccgaaacaga aacctgtact
     7561 ggttggagtc atgctcaaat gcagaactta tatgaagaag tctatgctgc ttgggaaccc
     7621 tttgcacacc tacctagccg ccttccacct gaattaagcg caaaacatga gaaactttat
     7681 ggccaagcga ttacgattgc caaacgtaaa ggctggtcag cagaacttgg ggaagacgat
     7741 taatatcaac taaccttaag aatcaatctc acctactact cctaactatt aagtattgat
     7801 cgctggaaac ggaatgattt gtgcaggcga tttgatagtc gctttatttg cgagaatttg
     7861 tagtgcactc ggaaattcct tttgaagccg gagcatcagt tcttcttgat tctctcgata
     7921 gtaaacaact tcgtaagcca ctggttgatc taaggaagta cgattttggg tttggaaatt
     7981 ctgccaggca atgtcaaccc aacattttcg ctcgccatcg ataaaaacaa attccttttt
     8041 atcgagaata gctaaatact gcatagcgcg aatcggcacg aataaatgat ccgtggtaca
     8101 ccgtgtgatt agctgacatg ctaaattata agtcgtagcg gggagaagac gcacctcacg
     8161 tttaatttct tggctacggt aacaagtaat atccatcatt ctttctccct atattcattt
     8221 acattaaatc taatgcgcgc gactcgccta aataaacctc ctgccaatcg gaatgattgg
     8281 aaaacaattc gctccctatt accctattta tgggaattta aaggccgtgt tctcattgcc
     8341 ttaatgttac tgctactcgc aaaacttgcc aatgttagcg taccattgat ccttaaagaa
     8401 atcatcgatg cgctagaccc tgcacaaacg atagtaacgc taccactttt tttactgatt
     8461 gcttatggtg tcttacgatt ctgcagtacc ttatttggcg aattacgtga cgccattttt
     8521 gcgaaagtaa cacagcgcgc gattcgacgt gtcgcaattc gagttttcag tcatttacat
     8581 tcattatcgc ttcgttttca tttaacaaga caaacaggcg gtgtatcccg tgatatcgaa
     8641 cgtggttcgc gtggtatctc gtttttattg aatttcatgc tcttcaatat tctaccgacg
     8701 ttacttgaaa tcagtctggt catggtcata ctcatttcta aatatgatat ttggtttttt
     8761 atcataactt ttataacgtt actttcttac atcgcgttga ctgtttttgt caccgaatgg
     8821 cggatgattt ttcgccgcac catgaacgat atggactcaa aagccaatac ccgcgcgatt
     8881 gatagcctgt tgaattacga aaccgtcaaa tacttcaata acgaagcttg ggaaactcag
     8941 cgctacgatg aacacctaca acaatgggaa aaagccgctg ttcgtaatca aacctcgcta
     9001 gctaccctca acacgggtca aagcgcaatt atcgcaatcg gcgttactct gctcatgatg
     9061 ttggctgcga gtaaagtagt ttctggcgaa atgaccttag gtgatttagt gttagtcaac
     9121 gcatttctct tacaactcta tatgccactc catttcttag ggtttgttta ccgtgaaatc
     9181 aagcattctt tagctgacat ggaaagaatg tttacgctat tagctgagaa tgaagaaata
     9241 caagatcaac ctaatgcaat cgcattaaat atttctcacg cctcaatcac atttaaaaat
     9301 gtcaatttta gttatgaatc caaccgccaa attctttttg atgtcaactt tagcattcct
     9361 tccggcagca cactggccgt cgttggtcat agtggctctg gaaaatccac attagcacgc
     9421 ttattgtttc gtttttacga tacatcgagc ggtgcaattc taatcaatgg ccatgacatc
     9481 aaacagatca cacaaaaaag tttacgctcg atgattggca tcgttcccca agacaccgtg
     9541 ctgtttaatg aaagcattta ttacaatatc gcttatggaa gacccgacgc atcagaagca
     9601 gaaattctag cagccgctaa atcagcccat atccatgatt ttgttcaaag ccttcccaat
     9661 caatacgact ctcaagtcgg tgaaagagga ttaaagctat caggtggcga aaaacaacgt
     9721 atcgccattg cccgagccat cctcaagaac ccattgattc taatttttga tgaggcaaca
     9781 tccgcactag attctagaac tgagaaaaat attcagcacg aactcaaaca attatccaaa
     9841 aatcgaacta cgcttatcat agcgcatcgc ctttcgacag ttattgatgc tgatcaaatt
     9901 ttagtgatgg ataaagggcg catcgttgag caaggaaacc atcaacaact cctcgctacc
     9961 aatggaattt acgctcagtt atgggaaata cagcaaaggg aagaagatac aacgggatcc
    10021 attacgattt gattaatgct catttcgctc gcatcaacta tgagctttat cacgattcct
    10081 tcacaaatca caccgtacaa tcttagccat gtaatttatt tcattcacat taacgttaga
    10141 gcaatgtgaa tgaataattt ttcttgacat ctgaataagg tttttttatc gtcagttaaa
    10201 tttgaaaaat aacatcactt ataggggaag aaatatgctt gatagtataa agaagatgtt
    10261 acagcagcta atgggaagta gcagtgaaga ttcaaatcaa gcaacagata taacatttta
    10321 tacactcggc aaatttaatt tgggtgtcgg taaaattgga cctgataatc ggacagatat
    10381 cgaccaaagc gtattaaaag aaatgtaccg ccgtgtagca gctggcgaat ttaatatccc
    10441 tgtttcaggg accttaccag aaatttgtgt tgatggccga accaatcgac aaggttcacg
    10501 aatcaatgcg ccttctgcag caggtggaag tttaacgatg gtgtatggcc gagatcttgg
    10561 tgactctggc gcattaaatg ataaaactga aatgcaatta accattgaaa ccatggatac
    10621 cctcaaagga aaagggtata caaccgcagt gcacggcgat gatcatagta attgtgggtg
    10681 cggtgcctgt gctcatgcac aaaaagttta tcaacatatt actgataaaa tcgatccgat
    10741 cgctaattta actggaaatt atggcattgt aatcagcgac actgaaaaag cgtccattat
    10801 gggtaaagca aaaaatcgtc tgaatcatcc aagctttttt gctgaagatc gatccgccat
    10861 catacaggcc tgtcaggatc atggtgcgaa ttatgaggaa ctcgttgatg c
//
LOCUS       DAOQKQ010000087        10808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_2878_length_10808_cov_6.230075, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000087 DAOQKQ010000000
VERSION     DAOQKQ010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 10808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10808
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2878_length_10808_cov_6.230075"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..1017)
                     /gene="ttcA"
                     /locus_tag="PKZ49_07075"
     CDS             complement(91..1017)
                     /gene="ttcA"
                     /locus_tag="PKZ49_07075"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HND36363.1"
                     /translation="MQEILSKKTQYNNNKLHKRLRRLVGTAIADFNMIEPGDRIMVCL
                     SGGKDSYGLLDILLNLQAHAPLDFELVAVNLDQKQPGFPEHVLPEYLARISVPFRIVE
                     QDTYSVVKRVISEGKTTCSLCSRLRRGVLYRVAKELGATKIALGHHRDDILETFFLNL
                     FYGGKLKAMPPKLVSDDGNHIVIRPLAYCKEKDLAAYAQVANFPIIPCNLCGSQPNLQ
                     RQVIKEMMQQWDKKFPGRIETMFTALQNIQPSHLADIARYDFQGLKAHGKADADGDKA
                     FDEEQFEPTIAALMPFSDDSSAYQVASTQDNT"
     gene            1203..1661
                     /locus_tag="PKZ49_07080"
     CDS             1203..1661
                     /locus_tag="PKZ49_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family protein"
                     /protein_id="HND36364.1"
                     /translation="MKKSLFMLYFILISWTLNTFADTGTHPEHSLIGQSGDAAQVSQT
                     IEIKMIENRFIPDEITIKQGETIRFLVKNDGKKHHEFAIDTIENLKEHAKMMRAHPNM
                     AHNDPNRIKIAPGEQKELIWQFTETGIVDFACPFPGHFKGMRGKIYVEKK"
     gene            1658..2122
                     /locus_tag="PKZ49_07085"
     CDS             1658..2122
                     /locus_tag="PKZ49_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HND36365.1"
                     /translation="MKTNKRQFILTSILTIHLVITTNFVSAAGTVEVYKSPTCGCCGK
                     WVDHLRDHGFTVNTHDVGNKEARARAGISPTLGSCHTALVDGYAIEGHVPAQDIIRLL
                     KERPKAVGISVPDMPHGTPGMEGERNDPYDVLLIQEKGDTRDKASVFNRYRP"
     gene            2135..2617
                     /locus_tag="PKZ49_07090"
     CDS             2135..2617
                     /locus_tag="PKZ49_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HND36366.1"
                     /translation="MKLQRCFNQAIMIASILFLSSCTESVTTATKLLQQLQEQGLMNE
                     VKRDFDAKQVTRGHEIFIKNCAVCHGQNGEGTADWRKPLANGRYPPPPLDSTAHAWHH
                     STEELKRFILKGGPPGEGRMPEWQDKLSEQEIDDILVWIKSLWSDEIYEAWYRQIEHR
                     "
     gene            complement(2648..2724)
                     /locus_tag="PKZ49_07095"
     tRNA            complement(2648..2724)
                     /locus_tag="PKZ49_07095"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2688..2690),aa:Arg,seq:ccg)
     gene            complement(2745..4034)
                     /locus_tag="PKZ49_07100"
     CDS             complement(2745..4034)
                     /locus_tag="PKZ49_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HND36367.1"
                     /translation="MEALSLPLLLLLLVVLLILSGFFSLSETSMMAINRYRLKHLAKQ
                     GHRGAQLASKLLENTDRLLGVILLGNNLLNTAAATLVAVIVSMLFAHSDLALLIGTLA
                     VTFAILVFSEITPKVIAATYPEKIALAASYILTPLLKIFYPAVWFVNLFVQGILSLLR
                     LHPKEHTAHKISPEELKMLVLEAGHFIQTKHQNILVNLVDLETVTVDDVIVPRKQIEA
                     IDLDAEDEVIHEQIMTCHHTRLPVYRERLDNVIGFIHVRKIFNQLKGERITANIIERV
                     LQEPYFIPSGTRLFSQLQLFQENRKRIGFVVDEYGEWLGLVTLEDIIEEIIGEFTTHA
                     PTQTSAFIQEPDGSIIAEGSVLLRDLNRKLGYQLPLDGPKTLNGLILEHFQDIPEAGI
                     GLNIAGYPMEIIQTKDQAVKIVRIHPTIPKIVGDKIE"
     gene            complement(4132..4341)
                     /locus_tag="PKZ49_07105"
     CDS             complement(4132..4341)
                     /locus_tag="PKZ49_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HND36368.1"
                     /translation="MGKIVIDIYESEHTKFMRELFKQRPHLVEQQKEARAIWWDKKVD
                     LETQKHFKEAKVPQTSYVYFDWLQK"
     gene            4684..6603
                     /gene="mnmG"
                     /locus_tag="PKZ49_07110"
     CDS             4684..6603
                     /gene="mnmG"
                     /locus_tag="PKZ49_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HND36369.1"
                     /translation="MVFSEKFDIIVVGGGHAGTEAALAAARTGRKTLLLTHNLDTLGQ
                     MSCNPSIGGIGKGHLVKEIDALGGVMAEAADEAGIQFRTLNSSKGLAVRATRAQADRV
                     LYRQAIRSRLENQPNLWLIQQSVDDFILEGDKIAGVVTQLGIRYSARAVILTAGTFLA
                     GLAHIGSAHFQAGRAGDPPSISLAHRLRELNLPVGRLKTGTPPRLDGRSINYALLQEQ
                     PGDYPTPVFSFIGNVSQHPRQISCWISRTNDQTHEIIRNGLDRSPLFTGKIEGVGPRY
                     CPSIEDKVVRFSERNSHNIFLEPEGLNTHEIYPNGISTSLPYDVQIELVRSIAGLEHA
                     HITRPGYAIEYDYFDPRALKSSLETKAIEGLFFAGQINGTTGYEEAAAQGLLAGLNAS
                     LKLQGHEPWCPLRNEAYLGVLVDDLITRGVTEPYRMFTSRAEYRLQLREDNADMRLTE
                     IGYRLGLVSEARWRTFSHKQATIELEQQRLRSYWVRPGMAPEEHQVKILGNSIEKEYT
                     LYDLLRRPNVTYDGLMTLFQIEDNVSNSSVAEQVEISAKYEGYIERQRQEVARHQHYE
                     TTRLPIGIDYHQVSGLSNEVRQKLNLHKPETIGQAARISGVTPAAISLLLVHLKRGFI
                     LSNSNQDYESGKTTA"
     gene            6578..7225
                     /gene="rsmG"
                     /locus_tag="PKZ49_07115"
     CDS             6578..7225
                     /gene="rsmG"
                     /locus_tag="PKZ49_07115"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HND36370.1"
                     /translation="MNLEKQLLEGLEAINCGTSDRKALCTRLLHYTALIEKWNQVHNI
                     TAVRKPEEMVSKHLLDSLTLLPHIIGQYVADVGSGAGLPGIPLAIANPDLRVTVIESN
                     QKKIAFLDQARLELALSNVKVTPVRVEKYFPTQRYDTVVSRAFSDLATFVKSAQHLCV
                     EDGKSRLIAMKGRFPDAELMQLPENCIVERVIRVNVPGLRAARHLIIIKPEQRTH"
     gene            7245..8009
                     /locus_tag="PKZ49_07120"
     CDS             7245..8009
                     /locus_tag="PKZ49_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HND36371.1"
                     /translation="MVKILAIANQKGGVGKTTTSINLAASLVNAGKRILLIDLDPQGN
                     TTMGSGIDKRFLQHTIYHVLLGEQSIANVRISETKGRYHLIPANQDLAGAEIEMVGLE
                     QRETRLKNALQEVIADYDLVLMDCPPALNLLTLNGLCAAHAVMIPMQCEYFALEGLSD
                     LVNTIKRVRASFNPALKIEGLLRTMFDPRNILAKQVSDQLQQHFGKKVYQTVIPRNIR
                     LAEAPGFGLPVLLHDKHSKGAQAYLDLAKEVLNAAA"
     gene            8053..8889
                     /locus_tag="PKZ49_07125"
     CDS             8053..8889
                     /locus_tag="PKZ49_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HND36372.1"
                     /translation="MKLKGLGRGLDALLTGSSINQALSESLQNLDIAQLQPGKYQPRT
                     NMDQSSLMELSKSIKAQGIMQPILVRAIDTERYEIIAGERRWRAAQLAGLTQVPVIIR
                     EVADESALAMSLIENIQRENLNPLETAMGIERLIREFGMTHQTAGDALGSSRSAITNL
                     LRLLNLASPVQDLMMQGKIDMGHGRALLTLNATKQIEIAHLIANKQLSVRETEKLIKR
                     IEQVPLKKPIIQNRDLLRLQEDISTKLGANVTIKPGKHGTGNLVIHYTSLEQLDGILA
                     KF"
     gene            8936..9931
                     /locus_tag="PKZ49_07130"
     CDS             8936..9931
                     /locus_tag="PKZ49_07130"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HND36373.1"
                     /translation="MDTHVLANPKLAAILGGLVADAASLGLHWLYDPNRIAEIEKESD
                     IAFRQPNENDYANVSGFFAHGSKVAGDSSGYGEIGLLMLKHLAKHGQFNHMAYQLEFR
                     AFFGPGGEYTGYVDSPTRQTIRTLLPLDAKSFPATSGADDDQMLALATVPVLVATHHG
                     TVDSLMKQVEEVVRITNHNDIAVSAAQCAAALLNGILMGKPLNQAATDALPLAEDNLR
                     LLLEQALSLEALDSVAAANRFGSACHVTEGLPIVFHIAKHAHDYRTAINANIRAGGDS
                     CGRSIILGALVAAHLAAQNRANTGIPLPWLARYRKLTMAADACQAIMDVPTPKAR"
     gene            complement(10299..10661)
                     /locus_tag="PKZ49_07135"
     CDS             complement(10299..10661)
                     /locus_tag="PKZ49_07135"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HND36374.1"
                     /translation="MSNGDDTIISCFVVGIAGCSDNEMGFLFREEGITAASPGVFTNV
                     QPNDYWSVTEFAIPFSAWNFNFNNGNRFVDGMNFNFFAWAVHSGDVGAQVPEPGTLAL
                     MGLGLMGLLGFGRQQRRR"
BASE COUNT         3225 a         2392 c         2280 g         2911 t
ORIGIN      
        1 gtttgcacta ctcgttctga tgacctataa agttgcacca cccattgctt ataaaagaaa
       61 ccataatcgc cattagttta tttaaaacca ctatgtatta tcctgtgtcg aggcaacttg
      121 ataagcggat gaatcatcag aaaatggcat tagcgctgca atcgtaggct caaattgttc
      181 ttcatcaaat gccttatcgc catctgcatc ggcttttcca tgcgccttaa gcccctgaaa
      241 atcatagcgc gcaatatcag ccaaatgtga aggttggata ttctgtaacg cggtaaacat
      301 tgtttctatt cgacccggaa atttcttatc ccactgctgc atcatttcct tgatgacttg
      361 tcgttgcaaa ttgggctgag aaccgcacag attacaagga ataattggaa aattggcaac
      421 ctgtgcgtag gctgctaaat ccttttcctt acaataagcg aggggacgaa tcacgatatg
      481 attcccatca tcgctaacta attttggtgg cattgcctta agcttaccgc cataaaatag
      541 attgaggaaa aacgtctcaa gaatatcatc ccgatgatga cccagtgcaa tcttagttgc
      601 cccaagctct ttcgcaacac gatacagaac acctctacgc aaacgagaac ataagctaca
      661 ggttgttttg ccctcgctaa tcactcgctt tactacacta taagtatctt gctcgacgat
      721 acgaaaagga acactaattc gagctaaata ttcaggtaag acatgctctg gaaaaccagg
      781 ttgcttttgg tccaaattaa cggcaaccaa ttcaaaatca agcggagcat gcgcttgtaa
      841 atttaacaaa atatccaata atccataact atcttttccg ccggataaac aaaccatgat
      901 ccgatctccc ggctcaatca tattaaaatc agcaatcgct gttccaacca gtcggcgtaa
      961 gcgtttatgt agcttattgt tattatattg tgtcttcttt gataatattt cttgcataag
     1021 taagccttta gtgatcatcc atcgtgcgat agcgtctagt gcgatcttta tagcagttct
     1081 gctagttgct gatataggta agattataca actcaaaact ggaacatttt aggatagact
     1141 atattctaaa taaggtaagt agccgtaaca gtcgattcat ctcaaaaaca aagggggtct
     1201 atatgaaaaa atctctattc atgctctatt ttattttgat aagttggaca ttgaatacat
     1261 ttgcagatac aggcactcac cccgaacatt cactgattgg acagtccggc gatgctgcac
     1321 aagtatctca aaccatcgaa atcaaaatga ttgagaaccg cttcataccc gatgagataa
     1381 cgattaagca gggtgaaacg attcgcttcc ttgtaaaaaa cgatggaaaa aaacatcatg
     1441 agtttgcgat cgataccatt gaaaatttaa aagaacacgc caaaatgatg cgtgctcatc
     1501 ccaatatggc gcataacgat cctaaccgta taaaaattgc gccaggagaa caaaaagaat
     1561 taatctggca gtttacggaa actggtatag tagattttgc atgccctttc cctggacact
     1621 ttaaagggat gcgtggaaaa atttatgtgg agaagaaatg aaaacgaaca aaagacaatt
     1681 catacttaca tcaatactaa caattcattt agtcatcaca acgaatttcg ttagtgcagc
     1741 aggaactgtc gaagtttata agagcccaac ttgtggctgc tgtggcaaat gggtggacca
     1801 cttacgtgat catgggttca ccgttaacac gcatgatgtc ggaaataaag aagcaagagc
     1861 gcgcgctggc atatccccaa cattaggatc atgccataca gcgctagttg atggttatgc
     1921 gatcgaaggt cacgtaccag cacaagacat tattcgattg cttaaagaac gacctaaagc
     1981 agttggaatt tccgttccgg atatgccgca tggcacgcca ggtatggaag gggaacgaaa
     2041 cgatccttat gatgtactac tcattcaaga aaaaggcgat acgcgtgaca aagcgtctgt
     2101 gttcaaccgt tacagacctt aatatcgctt atcaatgaaa ttgcagcgct gctttaatca
     2161 ggcgataatg atcgctagta tcttgttttt aagtagttgt acagaatccg ttacaaccgc
     2221 aacgaaacta ctccagcagc tccaggagca aggtttgatg aacgaagtga aacgtgattt
     2281 tgacgctaaa caagttacac ggggccatga aatttttatt aagaactgtg cagtttgcca
     2341 tggtcagaat ggtgaaggaa cagccgattg gcggaaacct ttagcaaatg gtagatatcc
     2401 gcccccaccg ctcgacagta ctgcgcatgc ttggcatcac tctactgaag aattgaagcg
     2461 cttcatcttg aaaggagggc ctcctggtga aggaagaatg cctgaatggc aagataagct
     2521 atccgaacaa gaaattgacg acattcttgt ttggattaaa tcattgtggt ctgacgaaat
     2581 ttatgaagct tggtaccggc aaatcgagca tcgctagttt atttttttga aaagcaggta
     2641 agtcatatgg cgcgcccaac acgattcgaa cgtgcgaccc ccgccttcgg agggcggtac
     2701 tctatccaac tgagctatgg gcgcgtggct gattagggaa taatctactc aattttatca
     2761 cccactattt tgggaatagt tgggtgaata cgaacaatct taacggcttg atctttggtt
     2821 tggataattt ccattggata acccgcgata ttcaatccta tccctgcttc cggtatatct
     2881 tgaaaatgct ctagaattaa accatttaaa gttttaggtc catctaaagg cagctgatat
     2941 ccaagtttgc gattaagatc acgcaataac acgctacctt cagcaattat actaccatct
     3001 ggttcctgaa taaatgcgct cgtttgcgtg ggcgcatgcg ttgtaaactc gccgataatc
     3061 tcttcaatga tatcttctaa tgtgacaaga cctagccatt caccatactc atcaacgaca
     3121 aaaccaatcc gttttcgatt ttcctgaaat aactgtagct gcgaaaacaa gcgtgtccca
     3181 gaagggataa aatagggctc ttgcaacact cgttcaatta tgttcgctgt gattctttcc
     3241 cctttcagtt gattaaaaat tttgcggaca tggataaaac caatcacatt atctaagcgc
     3301 tcacgataaa ctggaagccg tgtatgatgg caagtcatga tttgctcatg aataacctca
     3361 tcttctgcat ctaaatcaat tgcttcaatt tgtttgcgcg ggacaattac atcatccacg
     3421 gtaaccgttt caagatcaac caaattgacg agaatatttt gatgcttcgt ttgaataaaa
     3481 tgccccgctt ccaggacgag cattttaagc tcttctgggc tgattttatg cgcggtatgt
     3541 tccttgggat gtagtcgtag caagcttaaa ataccttgta caaacaaatt gacaaaccat
     3601 actgcggggt aaaagatttt gagtaacggc gtcagaatgt aactcgctgc aagcgctatc
     3661 ttttctggat aggttgcagc aatcacttta ggcgtaattt cactgaacac taaaatagca
     3721 aacgttactg cgagagtacc aatcaacaag gccagatcgc tatgggcaaa caacattgaa
     3781 acaatcactg caaccaaggt agccgccgca gtattcaata gattattacc taaaagaata
     3841 acgcctaata agcgatcggt attctcaagt agcttactcg caagttgcgc gccacggtgc
     3901 ccttgttttg caagatgctt caaacgatag cgattgattg ccatcatact ggtttcagaa
     3961 agcgaaaaga aaccagatag gatcaataaa acaaccaaca gcaaaagcaa taacggtaaa
     4021 gaaagcgctt ccaaagaatg ctatctcttt taatttatga ccgaggcgct ataaagtcag
     4081 caagtcaaaa tcctgattgc tcaccgatag caaaataaac gatggggtag attacttttg
     4141 cagccaatca aaataaacat agctcgtttg cggtacttta gcttctttaa agtgcttttg
     4201 tgtctcaaga tcaacttttt tatcccacca aatcgctctc gcctcttttt gctgctcaac
     4261 aagatggggt ctttgtttaa aaagctctcg cataaattta gtatgctctg attcatatat
     4321 atcgatgact atcttgccca tttatatttc cttattgttc taatgcaatt aacacttatc
     4381 ataatgacaa tgaagcaaat cacgcttctt aagtctagct cgctaggata actcataata
     4441 gcatatcttc agcgaatcaa cttatcatct aaacgcactt aaagatgata gtttattttt
     4501 ttcaactagt taaattatat tgtgacttgc ttttttaaca agaaaattaa aactgctggc
     4561 aaaaggtttc acgtgaaacc ttttttatcg ttgcaatata aaatacatat gtaatgagta
     4621 aaaataattt aaaatcttgt cttattctta aatttcacaa aacacccaaa tctgtgcaat
     4681 ccaatggttt tttcagaaaa atttgacatt attgttgtgg gcggggggca tgccgggact
     4741 gaagctgcct tggccgctgc acgcacagga cgaaaaacat tgctattaac ccataacctc
     4801 gatacgttag ggcaaatgtc atgcaatcct tcaattggag gaattggcaa aggtcacctc
     4861 gttaaagaga tcgatgcgct cggtggcgtc atggcagaag cagcagacga agcaggcatt
     4921 cagtttcgta cccttaattc gagtaaaggg ttagcagtgc gcgcaactcg cgcgcaggct
     4981 gatcgcgttt tataccgcca agccattcgc agtcgattag aaaatcaacc caatctttgg
     5041 ctcatacaac aatcggttga tgattttatt ttggaaggcg acaaaatcgc tggtgtagtc
     5101 acccaacttg gtattcgata ttctgctcgc gcagttatac taaccgcagg aacctttcta
     5161 gcagggttag cgcatatcgg tagcgcccat ttccaagccg gcagagcggg agatcctccc
     5221 tcaatcagcc tggcgcatcg gttgcgcgaa ttaaatttac ctgttggaag attaaaaacg
     5281 ggaacacccc cgcgacttga tggccggagt atcaattatg ctttacttca agaacaacca
     5341 ggtgattacc caacccctgt tttttcgttt atcggcaatg tgagtcagca ccccagacaa
     5401 atatcatgct ggatttcccg tactaatgat caaacccatg aaattatccg aaacggactt
     5461 gatcgctctc ctttgttcac aggaaaaatt gagggggttg ggccgcgcta ctgtccatca
     5521 attgaagata aggtagttcg attttcggaa agaaattccc ataatatttt tctcgagccc
     5581 gaaggattga atacacatga aatctatcca aatgggatct cgactagctt accttatgat
     5641 gtacaaattg aacttgtacg atcaattgca ggattggagc atgcgcatat tactcgccct
     5701 ggctacgcca ttgaatatga ttacttcgat ccccgtgcac tcaaaagctc cttagaaaca
     5761 aaagcgattg aaggattatt ttttgcgggc cagattaatg gcactaccgg ttatgaagaa
     5821 gccgccgcgc aaggattact agccgggctt aatgcaagcc taaaactcca aggacatgag
     5881 ccctggtgtc cgctcagaaa tgaagcttat cttggtgttc tagtcgatga tttaattact
     5941 cgcggtgtaa ctgagccgta tcgaatgttt accagtcgag ctgaatatcg attacaacta
     6001 cgcgaagata atgctgacat gcgcctgacg gaaattggtt atcgattggg gttagtcagt
     6061 gaagcgcgtt ggcgaacttt ttcccacaaa caagccacta tcgaactgga gcagcaacga
     6121 ttacgaagtt attgggttcg cccaggcatg gctcctgaag aacatcaagt caaaatcctt
     6181 ggcaatagta ttgagaaaga gtataccctg tacgacctct tacgcagacc taacgttaca
     6241 tatgatggtc tcatgacgct atttcagatt gaagataacg tgagcaattc cagcgttgct
     6301 gaacaagtag aaataagcgc aaaatatgaa ggctatattg aacgccaacg acaggaggtt
     6361 gctcgccatc agcattatga aacaacgcgc ttaccgatag gaatcgatta tcaccaggta
     6421 agtggattat ccaacgaagt tcggcaaaaa cttaatttac acaaacctga aacaatcgga
     6481 caagctgcgc gtatttctgg agttacacca gcagcaattt cattattgct cgtgcacttg
     6541 aagcgtggtt ttatattatc aaactccaac caagactatg aatctggaaa aacaactgct
     6601 tgaaggcctt gaggctatta actgtggcac atcagatcgg aaagcgttat gcacacgctt
     6661 gctgcactat accgctttga ttgagaaatg gaatcaggtt cataatataa cagcggtgcg
     6721 taaaccggag gaaatggtta gcaagcattt gctagatagc ttaaccctat taccgcacat
     6781 tattgggcaa tatgtagctg atgtggggag cggtgctggg ttacccggta tcccgctggc
     6841 aatcgctaat ccggatctcc gcgttactgt tatcgaaagc aatcaaaaaa agattgcttt
     6901 tttagatcaa gcgcggcttg agcttgcact aagtaatgtg aaagttactc ccgttcgtgt
     6961 tgaaaaatat tttccaacac agcgatacga taccgtagta tctcgggctt tttctgatct
     7021 tgccactttt gttaaatcag cacagcattt atgtgtcgaa gatggtaaaa gtcggttaat
     7081 cgcaatgaaa ggtcgattcc cagacgccga gttgatgcaa ttgcctgaga attgtatcgt
     7141 tgaacgtgtg attcgtgtca acgttcctgg tctaagggct gcacgccatc tcattataat
     7201 caagcctgaa cagcgtaccc attaaccatg aaggagttta tcaagtggta aaaatacttg
     7261 cgatcgccaa tcaaaaagga ggggtaggaa aaacgacaac cagcattaat cttgcggcga
     7321 gcttggtcaa tgcaggcaag cgcattttgc taatcgatct cgatcctcaa ggaaatacca
     7381 ctatgggaag tggtatcgac aagcgatttt tgcagcatac tatttatcat gtattgcttg
     7441 gagaacaatc gattgccaat gttcgtatta gcgaaaccaa aggtcgctac catctgattc
     7501 cagcgaatca agatcttgct ggtgccgaaa tcgaaatggt cggcctcgag caaagagaaa
     7561 cgcgattaaa aaatgcattg caagaggtaa tagcagatta tgatctggtt ctgatggatt
     7621 gcccacctgc gctcaattta ttgacgctga atggcctctg cgctgcccat gctgttatga
     7681 ttcccatgca gtgcgagtac tttgcactag aggggcttag cgatttagtc aacacgatta
     7741 aacgtgtccg cgctagtttt aacccggcgc ttaaaattga gggtttatta cggaccatgt
     7801 ttgatccgcg caacatctta gctaaacaag tctcagatca gctgcaacag cattttggaa
     7861 aaaaagtata tcaaaccgtt attccacgca atatccgctt agcggaagct cctgggttcg
     7921 gcttgcccgt actcttacac gacaagcatt ctaaaggtgc acaggcttat ctagatttgg
     7981 cgaaagaagt tttaaatgcg gcagcctaat cgtttttatc aagacgcaat aaaattctga
     8041 gggctcaatt taatgaaact aaaaggctta ggtaggggat tagatgcatt actaaccggt
     8101 agttcaatta atcaagcttt gtctgaatcc ttgcagaacc ttgatattgc tcagcttcaa
     8161 ccagggaaat atcagcctcg taccaatatg gatcagtctt cattgatgga attatctaaa
     8221 tccatcaaag cgcaaggaat tatgcagccg attttggtgc gtgctatcga tacagagcgt
     8281 tatgaaatta ttgcgggcga aaggcgttgg cgcgctgcac aattagctgg attaacacaa
     8341 gttccggtaa ttatccgcga agttgctgat gaatcggcgc ttgcgatgtc gcttatcgaa
     8401 aatattcagc gggagaatct caacccactc gaaactgcca tgggtatcga gcgcctcatt
     8461 cgcgagtttg gtatgacgca tcaaacagca ggggatgcgt tgggtagctc aagaagcgca
     8521 attaccaact tattacgtct attgaatctt gcgtcacccg ttcaggatct catgatgcaa
     8581 ggaaaaatcg atatgggaca tggccgtgcc ttacttaccc ttaatgctac taaacaaatt
     8641 gaaattgccc atcttatcgc gaataaacaa ctttccgtca gagaaaccga aaaattaatc
     8701 aagcgtatcg agcaagttcc attgaaaaaa cccattattc aaaatcgcga cttattacgc
     8761 ttacaggaag atatttctac caaattaggt gccaatgtca caattaagcc tggcaagcat
     8821 ggaacaggta atctggtgat tcactatact agccttgagc aactcgatgg tattctcgcc
     8881 aagttttaac caaaacaatt gtacgctttt ctaattcatt cacctggagt cagcattgga
     8941 tactcatgtt ttagcaaacc ccaaacttgc cgccatttta ggtggattag tcgctgatgc
     9001 tgcttcatta ggccttcatt ggctatatga tcctaaccgg attgcggaaa ttgaaaaaga
     9061 atctgacatc gcctttcgtc aaccgaatga aaacgattat gccaatgtaa gcggcttctt
     9121 tgcgcatggt agcaaagtcg caggagattc atcaggttac ggagaaatcg gtttactcat
     9181 gctcaagcac ctagccaagc atgggcaatt taatcatatg gcttatcagc tcgaatttcg
     9241 cgctttcttt ggccccggcg gagaatacac gggttatgtc gattctccca cgcgccaaac
     9301 catacggact ttgttgccac tggatgccaa aagttttcca gcgacttccg gagccgatga
     9361 tgatcaaatg ctggccttag caaccgtacc cgttttagta gcaacacacc acggaaccgt
     9421 tgattcactg atgaagcaag tagaagaagt tgtgcgcatt actaatcaca acgatattgc
     9481 tgtctcagca gcccaatgtg ccgctgcatt gctcaatggt attttaatgg ggaaaccctt
     9541 gaatcaagcc gcaaccgatg cactaccctt agccgaagat aatttgcgat tattactcga
     9601 acaagcactc tcgctagaag cattggatag tgttgcagca gccaaccgtt ttggcagcgc
     9661 ttgccacgta acggaaggac ttccaatcgt ttttcacatt gcaaaacatg cccatgatta
     9721 taggacagcc attaacgcta atattcgggc aggaggcgat agctgtggcc gttcaattat
     9781 attgggtgca ttagtagcag cccatctcgc tgcacaaaat cgcgcaaata ctggaattcc
     9841 gttaccctgg cttgcgcggt atcgtaagct caccatggca gcagatgctt gtcaggcgat
     9901 catggatgta cctaccccta aggctaggta gaaaggttct agccaactac tgacagaatt
     9961 acagctaagt tttgtgtgtt ccttaaaaga ataggcgttt atacaacggc tgagagggtc
    10021 tgcgactggc tggaatcaat cttattcttc acgttaaaga agataatctt gatgatctgg
    10081 aaagggtaga gattaactta gagtatgaat aataaaaaac cccacagttt taaggtgggg
    10141 ttctcttcgt aattggtagg tcggaggttc gactccacac ccttcaaata cttacatcac
    10201 tgtaaaatat tccgataatt cccgatagga tcggcacagc aagggaggct tccacctgtg
    10261 cctattcaaa gagccgaagc gcccgaatca tcaaagtatc aacgtcgacg ctgttgccgt
    10321 ccgaagccaa gtagtcccat taacccgagt cccatcagtg ccagcgtacc tggctcaggt
    10381 acctgcgcgc cgacatcacc cgagtgaaca gcccaagcga aaaaattgaa gttcatgccg
    10441 tccacgaaac ggttgccgtt attgaagttg aagttccacg cgctgaatgg aatcgcaaac
    10501 tccgtaacgg accagtagtc gttgggctgg acgttggtga atactcccgg tgatgctgct
    10561 gttatgcctt cttcacggaa cagaaacccc atttcattat cagaacaacc agcaatccca
    10621 acaacaaaac aactgattat agtatcgtca ccattagaca tcaacgctag gtagccgcca
    10681 accgcctatt ccgccaatag taagacctga aacccaggct tgttgatcgt tccaagtacc
    10741 ttgccctgtc aatctgcatg ccaaattagc cgagtgggaa gctttctaca actatcaccg
    10801 acctcatg
//
LOCUS       DAOQKQ010000088        10755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_2896_length_10755_cov_5.373084, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000088 DAOQKQ010000000
VERSION     DAOQKQ010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 10755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10755
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2896_length_10755_cov_5.373084"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(491..2908)
                     /gene="lon"
                     /locus_tag="PKZ49_07140"
     CDS             complement(491..2908)
                     /gene="lon"
                     /locus_tag="PKZ49_07140"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HND36375.1"
                     /translation="MTSDAENKDSDSIVLPLLPLRDVVVFPHMVIPLFVGRTKSIKAL
                     ELAMESGKNILLVAQKSAAKDDPSLSDLYGIGCVSNLLQMLKLPDGTVKVLVEGNYRA
                     HIREYIDSENYLLGNIEEIPADNQNTPEIEAMRRALITQFDQFVKLNKKIPQEILASL
                     SSIDESGRLADTVAAYLPLKLEQKQEILEIVNTQKRLERLLNLLETELDILQVEKRIR
                     GRVKRQMEKSQRDYYLNEQVKAIQKELGEGEEGADLDEIEKKIKAASMPKEALAKAES
                     ELKKLKLMSPMSAEATVVRNYIDALVTLPWKHKSKICKDLSAAEAVLEEDHHGLEKIK
                     ERIVEYLAVQQRVSKLKAPILCLVGPPGVGKTSLGRSIARATNRKFVRMSLGGVRDEA
                     EIRGHRRTYIGSMPGKILQNLSKVGVRNPLFLLDEVDKMGMDFRGDPASALLEVLDPE
                     QNNAFVDHYIEVEYDLSDVMFVATANSLNIPPALLDRMEVIRLSGYTEDEKLNIAERY
                     LLPKQMTNHGLKEDELSISHSALCNIVRHYTREAGVRSLEREISTICRKVVKTILTQK
                     KKGKQQVVVNARNLDKYLGVTRYTYGTAESKNQVGQVTGLAWTEVGGELLTIEAAVLP
                     GKGKTITTGKLGEVMQESVQAALSVVRSRAAVLGIADEFYLKKDIHIHLPEGAIPKDG
                     PSAGIGICIAMISALTNIPVRATVAMTGEITLRGEVLPIGGLKEKLLAAHRGGIKTVL
                     IPEDNVKDLSEIPENIISKLKIKPVKWIDQVIDFALEYKPEIAAAPIPTPLSEGDENL
                     RTTVVKH"
     gene            3265..3684
                     /locus_tag="PKZ49_07145"
     CDS             3265..3684
                     /locus_tag="PKZ49_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36376.1"
                     /translation="MKKLIFAGLLSIITFHTAVNAKTEEPIQFAQLAPAKPAIEKPRT
                     TNMRENKKIMLNQEEVEKNAKQKQPSSSASGTPQADRLLGNRGYNDPTQTDNPGLGHD
                     RGFNKGKSEKDNNRFNNANPPNKIHRSNNIHMGVPAY"
     gene            3897..7358
                     /locus_tag="PKZ49_07150"
     CDS             3897..7358
                     /locus_tag="PKZ49_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[ACP]--phospholipid O-acyltransferase"
                     /protein_id="HND36377.1"
                     /translation="MQQNRLHLLSSKRFLPLFASQFLGAFNDNIFKNALIIYMTYVVI
                     DQSSVDPEIMVTLAAGIFVLPFFLFSSTAGRLADKWDKAKLIRIIKFIEIPLIIGAAA
                     AFLLHSIPLLMILLFLMGMQSTFFGPLKYGILPDQLLKNELIEGNALIEAATFLAILA
                     GTILGGLLILANNGTNLVAIVMIMTAFAGWLASLFIPSTPARSPEIKIGFNIWKDTQA
                     LLHHIQQNKIIFRSILGISWFWLLGATYLSQLPTFSKNIIGGNEQVVTLFLVIFSVGI
                     AIGSLLCNRLLKQEIAATYTPVGILGVTLFSIDLFFAAGQISPAENTALIGATEYLST
                     FTHWRILFDLLGISICGGIFIVPLYAIIQDCSETSHRSRVFAANNIINALFMVIAAIT
                     ISLMYYSNFSVTDCFLTLAILNGFVALYISTLLPHALVKSLLQALLYTCYRLEIKGLE
                     NYRDVGEKSIIVANHLSFLDAILLSVCIPDQLCFAINTHIAQKWWIRPFLFLAETVAI
                     DPTNPMSTRLLIDRVKKGKRIVIFPEGRLTVTGSLMKIYEGPAMIADKSGATLLPISI
                     VGAQYTPFSRLKGKLRIRWFPRITITIMPPEWLNLPNEVRGKQRRQLAGNKLYDIMTS
                     ALFHSNDLQQTLFQSLLDASHIHGRNHIIAEDIERQPITYSQLLTRSFILGSRLCKFS
                     KPAENIGLLLPNSIGAIVTFFALQAYARIPAMLNFSVSKANLMNACQIASIRTLVTSS
                     RFIEVAKLTSVIDNFRAMGITVLYLEDLRQEIHWWHKLLGFLAGLIPQTYYRLCCPAS
                     YPDTPAVILFTSGSEGLPKGVALSHVNLQANRFQVAARIDFGPTDIVFNVLPIFHSFG
                     LTGGTLLPILSGIKAFLYPSPLHYRAIPELIYDSNATILFGTDTFLTGYARHANPYDF
                     YSLRYVFAGAEKLRESTRRVWSENYGVRIFEGYGATETSPILSLNTPMHNRPGSVGRM
                     LPGIAYRLEKISGIEEGGKLLVSGPNIMMGYYLINAAGQINPPNQGWYDTGDIVAIDQ
                     DGYIFIKGRAKRFAKIAGEMISLTAVEAYISKLWPNYQHAVVAIPDSKKGEQLVLITT
                     NPTAGRNEILTYVNQQGFGELSIPKTILIKEDIAVLGSGKTDYVALLDWVNTQSTQTH
                     NKDQLND"
     gene            complement(7355..8758)
                     /gene="der"
                     /locus_tag="PKZ49_07155"
     CDS             complement(7355..8758)
                     /gene="der"
                     /locus_tag="PKZ49_07155"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HND36378.1"
                     /translation="MNPTLVLVGRPNVGKSTLFNRLTRGRDAIVADLPGLTRDRHYGQ
                     GKVGKKPFIVVDTGGFEPVVKSGILYAMAKQTLQAIDEADVILFVVDGRQGLTPHDKI
                     IAEQLRKSGQRIQLVVNKTEGMNTSLITSEFHELGLGDPCAVSATHGQYIAELVESAL
                     APFEFLAEEEKITLEAGPKIAIVGRPNVGKSTLINTLLGEERVIAFDQPGTTRDSIYI
                     DFQYHQRPYTLIDTAGLRRRGKVFEAVEKFSVIKTLQAIEDANVVVLVLDAQNDISDQ
                     DAHIAGFVLETGRALVVAINKWDGLDSYQRETIKREILRRFIFLDFAKFHFISALQAK
                     GIHELFLSIDTAYKAAMASLPTPKLTRVLHAAVMKQSPPRGGMSYPKLRYAHQGGSNP
                     PLIIVHGSMLNHVPETYRRYLENTFRDFFNLEGTPLRVEFRVGHNPYANKKPAPLTEE
                     EAKRAHSRRRRNRKKYG"
     gene            complement(8849..10102)
                     /gene="bamB"
                     /locus_tag="PKZ49_07160"
     CDS             complement(8849..10102)
                     /gene="bamB"
                     /locus_tag="PKZ49_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HND36379.1"
                     /translation="MISYTLVFFLVFEIKKIRQKFFSYFGLVLLTGCSSFGGLGGFGD
                     FGDFGISNLIDRFSSGKDEIQIDEEEIAALKALPTLPLIWKSKVSESEIAVFEPIYEN
                     GAIYTADEKGFLTRLDPLNGKETWQIKTKLKLSGGVGAGEGLVLVGTFNGEVHAFDES
                     GGALWKARLTSEVLSAPQVHRGIVVVRTGDGRVFGLDAADGSRKWVYQGVTPALTVRN
                     FAGALVARGAVFVGLSGGKLIAMNMLNGNIGWEAAVSQPKGVTELERMTDITSLPVID
                     DQQVCAVAYRGRIACFEIMDGNQIWARDASSHVGLVMDNNYIYVSEERGAVVAYDKRS
                     GAMMWKQAKLGTRKLSAPQLVGGSSLLVVADAEGNVTLLNRYDGNLVARATTDESPIY
                     SRPEYIPDGFIVQTSKGGIYAFLPQ"
     gene            complement(10106..>10755)
                     /locus_tag="PKZ49_07165"
     CDS             complement(10106..>10755)
                     /locus_tag="PKZ49_07165"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HND36380.1"
                     /translation="EIVAYNPEEQEKIEGLKAWWSTHGSSVIIILSTMIAVMAGMQAW
                     KYYHKQQALQAADLFAVLQQQIDKGGSSEKINDALHLLTTGYPESGYASRAALIAAQA
                     NKNLGNLPEAKAKLQWILDHAKELEIKDIARLRLAGVLLDEKKFDGALKLLDSQHGES
                     FSGLYADLKGDILVAAGKPSEASAAYQKAYDRFSMSKSNYMNLVQMKLDAVKVSK"
BASE COUNT         3016 a         2568 c         1996 g         3175 t
ORIGIN      
        1 aaaaatatcg cgtaaaactg ctctaattca tgacccatca actttagtcc catttctcca
       61 tttttgcgta ggctagcatc taagccgtta actcgattga tgaaataaaa actgaatcac
      121 atgacaattt caatttaagt ttctgtacgc tgacgctcaa ggcgtcaaac gctatacaaa
      181 tagagtgtgt taacgtaggg gggaaatgaa aatcaccctt ggcacgagta accagttttt
      241 tgtgccaggt gaaaattttt taggaaaaaa cttgaaacaa caaaatgaca gtctggcttt
      301 tcctagaaaa agtcaggtct taagatgaat aagcaatcca ccatcggttt actaccggga
      361 ttacaagaaa acaggaggcg taccctattt aacttcctgc ttaaacgctt tgttaaatag
      421 ggtaattgtt atcgagaacg atacgattga atttgtgttg cttttatgcg caacaccgtt
      481 agcttcacga ctaatgctta accactgtcg ttcttaaatt ttcatcgccc tcggacaggg
      541 gtgttgggat cggtgcagca gcgatttcag gcttatattc caaagcaaaa tcaatgacct
      601 gatctatcca cttaaccggt ttaattttta acttactgat aatattttct ggtatctcac
      661 tcagatcctt aacattatct tctggaatca gcacggtttt aattccacct cggtgagccg
      721 caagcaattt ctctttgagc cccccaatgg gtagcacttc accacgcaat gtgatttcac
      781 ccgtcatcgc cacagtcgca cggaccggaa tattggttaa tgcagaaatc atcgcaatac
      841 aaataccaat cccagcactt ggaccatctt tggggatagc accctctggt aagtgaatat
      901 ggatatcctt tttcaaataa aattcatcag caatgcccaa aacagcagca cggctacgaa
      961 cgactgacaa agcagcttgc accgattctt gcattacttc acccagttta ccggtcgtaa
     1021 tggtttttcc ttttccaggt agaaccgcgg cttcaatcgt tagcaattct cctccgactt
     1081 cagtccacgc tagccctgtg acttgcccta cctgattttt actttcagcg gttccatagg
     1141 tatagcgcgt tacacctaaa tacttatcga gattacgagc attaaccacg acttgctgtt
     1201 tgcctttttt cttctgcgtc aggatcgttt tcacaacctt acgacaaatc gttgaaattt
     1261 ctctttctag cgatctaacc cctgcttctc gcgtgtaatg tcttacgatg ttgcataacg
     1321 ccgaatgaga aatagacaac tcatcttctt ttaaaccgtg gttcgtcatt tgtttaggca
     1381 gcaaatagcg ctcggcaata ttcagcttct catcttcggt atagccagaa agtcgaatca
     1441 cttccatccg atccagtaac gcaggcggaa tattaagcga atttgccgtt gcaacgaaca
     1501 ttacatccga taaatcatac tcaacttcaa tatagtgatc gacaaacgcg ttattttgct
     1561 caggatcaag tacttcaagc aatgcagaag ccgggtcacc gcggaaatcc atccccatct
     1621 tgtcaacttc atctagcaga aacaatggat tccgaacacc gaccttactg agattctgca
     1681 atatcttccc gggcatagag ccaatgtaag tgcggcgatg gccacgaatt tcggcctcat
     1741 cacgcacacc gcccaaagac attctgacaa atttccgatt ggttgcccgc gcaatggatc
     1801 gacctagcga agttttacca acaccggggg ggccaaccag acaaagaatc ggcgctttta
     1861 gcttacttac tcgttgttgt accgccagat actcaacgat tctttccttg attttttcta
     1921 acccgtgatg gtcttcttca agcactgctt cggcagcact caaatctttg caaatcttac
     1981 ttttgtgttt ccatggcaaa gtcaccaaag cgtcaatata atttcgcaca accgtggctt
     2041 ctgcagacat cggagacatc agtttcaatt tcttcaattc ggattctgct ttagcaaggg
     2101 cttctttagg catcgatgct gctttaatct ttttctcaat ttcatcgaga tcagcgccct
     2161 cttcaccctc accgagttct ttttgtatcg cttttacctg ttcgttcaga taataatctc
     2221 tttggctttt ctccatctgc cgcttaaccc ggccacgaat ccgcttctcg acttgcaata
     2281 tatctaattc agtttctaac aagttgagta agcgctcaag ccgtttctgg gtattaacga
     2341 tttcaaggat ttcttgcttc tgttcaagtt tcaaaggcag ataagctgca acggtatccg
     2401 ctaatcttcc tgattcgtca atgctcgaca gagacgctaa aatctcttga ggaatttttt
     2461 tatttaattt tacaaactgg tcaaattgcg taatcaatgc acgacgcatt gcctcgattt
     2521 ctggggtatt ctgattatcc gcaggtatct cttcgatatt gccaagtagg taattttccg
     2581 aatcaatata ttcacgaata tgcgcacgat aattaccttc aaccagcact ttgacggtac
     2641 catccggtaa ctttagcatc tgcaaaagat tagacacaca tccgatccca tataagtcac
     2701 tgagactcgg gtcatcttta gcggctgatt tttgtgctac cagcaaaata tttttgcctg
     2761 actccattgc taattcaagt gctttgatag acttagttcg accaacgaag agtggtatta
     2821 ccatatgcgg aaatacgacc acatcgcgta acggtagcaa aggcaacacg atcgaatctg
     2881 aatctttatt ttctgcgtct gaagtcatat ttatttataa atcacatatt aaattgtagt
     2941 taaagcatac tagactattg tttatatcac ttcaatctat tgcaacaaac ttgtatctcc
     3001 aattgcgagc tagatcaatt aattatttaa cccaatggtg ctaactaaca cgaccacatt
     3061 taattagacc accgaatgga tattcgacta tcgaaaacag gtgcacaaca caaattcact
     3121 ttattaatac tccctatttt tgtttttaca aatcgaattt acatggtaat ttatgggctt
     3181 atttgtagtt ttcaagattt gaaatatctt ttatcgtttt caagttctcg cttaattttt
     3241 ctaaccttat tagacaaaaa tacgatgaaa aaattaatat ttgcaggtct tcttagcatt
     3301 attacctttc ataccgctgt caatgcaaaa acagaagaac ccatccaatt cgctcaactt
     3361 gctcctgcta aaccggcaat agaaaagcca agaacgacta atatgcgtga gaataaaaag
     3421 atcatgctca atcaagagga agtagaaaaa aatgcaaagc agaaacaacc atcttccagc
     3481 gcgtcgggta cgcctcaagc agatcgatta ctcggcaatc gaggttataa cgatcccact
     3541 cagacggata accctggatt aggtcacgat cgaggtttca ataaaggaaa atctgagaaa
     3601 gacaacaatc gttttaataa cgccaatccc cccaataaga tccatcgcag taacaatatt
     3661 catatgggtg ttccagctta ttagaaaatc acccatcgca ttctaatctt caacgattac
     3721 cctccaataa ctcttcatcg atcgcggtta cgttctatct ggcgctaacc gccccatcta
     3781 tctaataata atctttatta ccgggtattc tttttttaaa acaatatcgt tccaatggat
     3841 aattgaaaaa gaaaaaaatc gactcacctc aaattttggg ctatttgttt aaacctatgc
     3901 agcaaaatcg attgcactta ctttcttcta agcgattctt accacttttc gcatcacaat
     3961 ttttaggcgc attcaatgac aatattttta agaatgcact gattatttac atgacctacg
     4021 ttgtgatcga tcaatccagc gtagacccag aaatcatggt tacgcttgct gcgggcattt
     4081 ttgtgttgcc attcttcctt ttttcgtcaa ccgcaggacg acttgcagac aagtgggata
     4141 aagccaagct tattcggatc attaagttca tcgaaattcc actgatcatt ggcgcagccg
     4201 ccgctttcct acttcatagc attccgttat tgatgatttt gcttttctta atgggaatgc
     4261 aatcgacttt ctttggccca ctgaaatatg gaatcttacc ggatcaatta ctcaaaaatg
     4321 aactaatcga gggaaatgca ctgatcgagg cggctacttt tttagctatc ttagcaggca
     4381 caatattggg tggcttactt attcttgcca ataacggcac caatttggta gccatcgtca
     4441 tgatcatgac agcgtttgct ggttggttag ccagtttatt tattccgtca accccagcca
     4501 gaagtccaga aattaaaatt ggattcaata tttggaaaga tactcaagcg ctccttcatc
     4561 acattcagca aaataagatt atctttcgct ccattctagg tatttcctgg ttctggttac
     4621 tgggcgcaac ctatctttca cagctcccaa cattttctaa aaatatcatt gggggcaatg
     4681 agcaagtcgt caccttattc ttagtcatat tttcagttgg gatagcgatt ggctctttgc
     4741 tgtgtaatag attacttaaa caggagattg cagccaccta tacaccggtg ggaattttag
     4801 gcgtcacgtt attctccatc gatctatttt ttgcggctgg gcaaattagc cctgcggaga
     4861 ataccgcatt aattggcgca actgagtatt tgagtacctt tacacattgg cgtatcttat
     4921 ttgacctact cggaatctcc atatgcggtg ggatttttat cgttccgtta tacgcgatca
     4981 tccaagactg ctcagaaacg tcccatcgct cccgcgtatt tgctgctaac aatattatca
     5041 atgcgctgtt catggtaatc gcagccatca cgatcagttt gatgtattac agcaactttt
     5101 cagtgacgga ttgctttctc acactcgcaa tactaaacgg cttcgtagcg ctttatatct
     5161 cgacgctttt gcctcacgca ttagttaaat cattgctgca agctctttta tacacatgtt
     5221 accgacttga aatcaaaggg ttagaaaatt atcgtgacgt aggtgaaaaa tccatcatcg
     5281 ttgccaatca tttatcgttt ttagatgcga ttcttttaag cgtttgtatc cccgatcaac
     5341 tttgttttgc catcaatacc catattgcgc agaaatggtg gataagacct tttctgtttt
     5401 tggctgaaac cgtagcaatt gatccgacca acccaatgtc gacccgatta ttgatcgacc
     5461 gggtcaaaaa aggaaaacgc atcgtgattt ttcctgaagg cagattaacc gtcaccggtt
     5521 ctctcatgaa gatttatgaa gggccggcaa tgattgccga caaatccggt gcaacgttac
     5581 tgccgattag tattgtcggc gctcaatata cccctttttc aagactcaaa ggcaaattaa
     5641 gaattcgctg gtttcctaga atcacgataa cgatcatgcc gcctgagtgg cttaatttac
     5701 cgaacgaagt tcgtggaaag caacgtcgac aacttgcagg aaacaagctg tacgatatca
     5761 tgacgtccgc tttgtttcat agtaatgatc tccaacaaac tttgttccaa tccctactgg
     5821 atgcctctca tatccatgga agaaaccata tcattgctga agatatcgag cgacaaccca
     5881 ttacctattc tcaattatta acccgtagct ttattttggg tagtcggtta tgcaaatttt
     5941 ccaaacctgc agaaaatatt ggactattgc tccctaattc cattggtgca attgtcacct
     6001 tctttgcact gcaagcgtat gcacgaattc ctgcaatgct taacttttct gtcagcaaag
     6061 ctaacttaat gaatgcttgc caaattgcaa gcattcgaac gcttgttacc tcgagtcgct
     6121 ttattgaagt tgcaaagctt accagcgtaa tcgataactt ccgagcaatg ggaataacgg
     6181 tactttattt agaagatctt cggcaagaaa ttcattggtg gcacaagctc ttggggtttc
     6241 tggcgggttt gatcccacaa acttactacc gattatgttg tcccgctagc taccctgaca
     6301 ctcctgctgt aattctcttc acatcaggct cagaaggttt gcccaaggga gtcgcactga
     6361 gtcacgttaa tcttcaagcg aatcgatttc aggttgcagc gcgcatcgac tttgggccga
     6421 cagatattgt tttcaatgtt ttaccgattt ttcactcttt tggtctaacc ggcggcacac
     6481 ttttacctat cctgtcaggt atcaaagcct ttctttatcc atcgccgctt cattatcgag
     6541 caattccaga gttaatctac gattccaatg caacgattct ctttggaacg gatacatttc
     6601 tcactggcta tgcgcgtcat gccaatccgt atgattttta tagtcttcga tatgtttttg
     6661 caggcgccga gaaattacga gaaagtacac gcagagtatg gtcggaaaat tatggcgtgc
     6721 gtatctttga aggctatggt gccacagaaa cttcacccat cctcagcttg aatacaccga
     6781 tgcataatcg accaggatcg gttgggcgta tgcttcctgg catagcctac cgactagaaa
     6841 aaataagcgg gatcgaagaa ggaggaaaat tattggtatc gggccctaat atcatgatgg
     6901 gctattattt aataaatgct gcaggccaaa tcaatccgcc taatcaaggt tggtacgata
     6961 ccggtgatat cgtagcaatc gatcaagacg gctatatttt tatcaagggg cgagcaaaac
     7021 gctttgcaaa aatagcaggg gagatgatat cgctaaccgc agtcgaagct tacatcagca
     7081 aactttggcc aaattatcag catgccgtcg tcgctattcc tgattcaaag aaaggggaac
     7141 aactcgtact catcaccact aacccaactg cgggtcgtaa tgaaattctc acttatgtga
     7201 atcaacaagg gtttggtgag ctgagtattc ccaaaaccat cctcatcaaa gaagatatcg
     7261 ctgttttagg ttcgggaaaa acagattatg ttgcattatt agactgggtc aatacacaat
     7321 cgacccaaac tcataacaaa gaccaactca atgactagcc gtatttcttc cggtttctgc
     7381 ggcgtcgact atgcgcgcgc ttggcctctt cttccgtcaa cggtgcaggt tttttgtttg
     7441 cataaggatt atgcccaaca cgaaattcaa ctcgcagcgg tgttccttcg aggttaaaga
     7501 aatcacggaa ggtgttttca aggtaacgac ggtaggtttc tgggacatga ttcagcatac
     7561 tgccatgaac aataatcaag ggtggatttg atcccccttg atgggcatac cgtaatttgg
     7621 gatacgacat tcctcctctg ggtggcgatt gtttcataac ggctgcatgc aaaactcgtg
     7681 tcaatttagg cgtcggcaaa ctcgccatgg ctgctttata agcagtatca atcgacaaaa
     7741 acagctcatg aatcccttta gcctgtaatg cagagataaa atgaaatttg gcaaaatcaa
     7801 gaaaaataaa gcgtctcaat atctctcgct tgattgtttc gcgctggtag ctatccaacc
     7861 catcccattt gttaatagcc acaactaatg ctctgcccgt ttctagtaca aaaccggcaa
     7921 tatgcgcatc ttgatctgaa atatcatttt gagcgtctaa taccagcact actacatttg
     7981 catcttcaat cgcttgtagc gttttgatta ctgaaaactt ctcaaccgcc tcaaaaactt
     8041 tgccgcgtcg ccttaatcct gcagtatcaa tcagcgtata gggacgctgg tgatattgaa
     8101 aatcaatata gatactatcg cgggtggttc ccggttgatc aaacgcaatg acgcgctctt
     8161 cgccaagcaa ggtattgatt aaagtagatt taccaacatt gggtctgccc acaatagcga
     8221 tctttggccc cgcttccagc gttatttttt cttcttctgc caaaaattca aagggagcca
     8281 aggctgattc cacaagctca gcgatatatt ggccatgtgt cgctgaaaca gcacaaggat
     8341 cacccaaacc caattcgtgg aattcagaag taatgagcga agtattcatt ccttctgttt
     8401 tattgacgac taattgaatt ctttgcccag atttgcgtaa ctgttctgca ataattttgt
     8461 catggggtgt taagccttgc ctaccatcca ccacgaatag aataacatct gcctcatcaa
     8521 tggcttgcaa ggtttgcttc gccatcgcat ataaaatacc ggatttcaca acaggctcaa
     8581 atccgcccgt atccacgaca atgaatggct ttttgcctac tttaccctga ccataatgcc
     8641 gatcacgagt taatccaggt aaatcagcaa cgatggcatc acgaccacgg gttaaccgat
     8701 taaataacgt agatttccct acattaggtc ggccaacaag cactaaagtg ggattcattg
     8761 cttagcgtaa aggctatagg agaattgttg cgtgaaagaa atgactgatt attaattaat
     8821 aacgaatcaa ctcgttttac tgccatgctt attggggtaa gaaagcatag ataccccctt
     8881 ttgatgtttg tacaataaac ccgtcaggaa tatattctgg tcgactataa atcggacttt
     8941 catcggtagt tgctctcgcg accaaattac cgtcatagcg attcaatagg gttacattgc
     9001 cttccgcatc tgcgacgact aacaaagaac tgccgccaac caattgtggc gcagaaagtt
     9061 tgcgagttcc taattttgcc tgcttccaca tcattgcgcc gcttcgctta tcataagcca
     9121 ccacggcacc ccgttcttca ctgacataaa tataattatt atccataacg agtccaacat
     9181 gactcgatgc atcccgcgcc catatttggt taccatccat gatttcaaaa caagcgatac
     9241 ggccacgata agcaaccgca caaacttgtt ggtcatcaat aacggggaga ctggttatat
     9301 cggtcattcg ttccagctca gttactcctt tcggctgtga aacagcagct tcccagccaa
     9361 tatttccgtt taacatattc attgcaataa gctttccacc agatagccca acaaaaacgg
     9421 caccgcgtgc aacgagcgca cccgcaaaat tcctaaccgt caatgcaggt gtaacccctt
     9481 gataaaccca tttgcgcgat ccatccgcag catctagtcc aaaaacacgg ccatcgcctg
     9541 ttcgaacaac gacaatgcca cgatgcactt ggggtgcgct taacacttca ctggttaaac
     9601 gcgctttcca taatgcaccg cccgattcat caaaagcgtg aacttcccca ttaaatgtcc
     9661 ccaccaagac taaaccttct cccgcgccga ctccacctga taatttaagt tttgtcttta
     9721 tctgccaagt ttcctttcca ttcagtgggt caagccgtgt taaaaatcct ttttcatcgg
     9781 ccgtgtagat agctccattt tcatagatgg gttcaaatac agcaatttcg ctctcactga
     9841 ctttagattt ccaaattaaa ggcagcgtcg gcaacgcttt taacgctgcg atttcttctt
     9901 catcaatttg aatttcatct ttgccactac tgaagcgatc gattagatta ctgataccaa
     9961 agtccccaaa atcgccgaat ccacccaatc cgccaaagct ggagcacccc gttaatagta
    10021 ctaacccaaa gtatgaaaaa aacttttgtc gtattttctt tatttcaaaa acaagaaaaa
    10081 aaactaacgt gtaactaatc acctgctatt tgctcacttt aactgcgtct aatttcattt
    10141 gaacgagatt catgtaatta cttttactca tactaaagcg gtcataggct ttctgatagg
    10201 cggcactcgc ttcactcggc tttcctgcag caaccaatat atcccctttc aaatcagcgt
    10261 aaagtccgct gaaagattct ccatgctgac tatccaataa tttcaatgcc ccatcaaatt
    10321 tcttctcatc cagcaaaacc ccagctaagc gcagccttgc gatatccttt atttccagct
    10381 cttttgcatg atcaagaatc cattgcaatt tagctttagc ctctggaaga tttcccaaat
    10441 ttttattggc ttgagcagct atcaatgctg cacgagaagc gtagccactc tcgggataac
    10501 cagtagtcag gagatgcagc gcatcattga ttttttctga gctaccgccc ttatcaatct
    10561 gctgttgcaa tactgcaaac aagtcggcag cttgcaacgc ttgctgctta tggtaatact
    10621 tccacgcttg cattccagcc ataactgcga tcattgtcga caaaatgatg atgacggagc
    10681 taccatgagt cgaccaccac gctttcaatc cttctatttt ttcctgttct tccggattat
    10741 atgccacgat ttctt
//
LOCUS       DAOQKQ010000089        10649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_2932_length_10649_cov_5.622333, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000089 DAOQKQ010000000
VERSION     DAOQKQ010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 10649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10649
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2932_length_10649_cov_5.622333"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="PKZ49_07170"
     CDS             <1..524
                     /locus_tag="PKZ49_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
                     /protein_id="HND36381.1"
                     /translation="RRGGALEMVQLWVNLPAKNKMSPPRYQSILDSQIPAIDLPQDQG
                     KIRVIAGEFGGLTGPARTFTPINVWDLRLSNGQLIDLAVPDGYNTLLAVLKGSMRVNN
                     SETINTAEVGLFDQTGDHIRITSTSDTTALLLCGKPIDEPIVGSGPFVMNTTEEIRQA
                     MADYQSGKMGKIT"
     gene            645..1247
                     /locus_tag="PKZ49_07175"
     CDS             645..1247
                     /locus_tag="PKZ49_07175"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HND36382.1"
                     /translation="MLLIDHQSGLFQLVKDIPVSDLRRNAIALAKVATILKMPVITSA
                     SVPDGPNGPLIPEIHQAAPHAQYVGRRGEINVWDSPDFVKAVEATGRKTLILAGTLTS
                     VCMAFPSISAVAAGYRVYAVVDASGNWSQMGTELTIARIVQAGVIPIDTVAVISELQK
                     TWNRPEAIEFADLYADYPMLHYRLLIESYDKAQTVQKAKN"
     gene            1264..1614
                     /locus_tag="PKZ49_07180"
     CDS             1264..1614
                     /locus_tag="PKZ49_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HND36383.1"
                     /translation="MTLEEKFNDFNQMILDGEILESMDKYYHSDCIVIEKNDVVATGL
                     QQAKARESELFEGVEAWLKSEIVSTAVGENVTMTEWHYEYKHKDFGHKKFDQVAVQHW
                     KNGKIISDRFYALY"
     gene            1653..2282
                     /gene="ycaC"
                     /locus_tag="PKZ49_07185"
     CDS             1653..2282
                     /gene="ycaC"
                     /locus_tag="PKZ49_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HND36384.1"
                     /translation="MNKTFKYNRLSKDDAVLLLVDHQAGLISLVQDFSPNEFKNNILA
                     LTACGKYFKLPTILTTSFEDGPNGPLVPEIKEMHPAAPYIARPGNINAWDNKDFVNAV
                     KKTDRKQLIIAGVVTEVCVAFPALSAIEEGYEVFVVTDASGTFNEVTRDAAWARMQSA
                     GVQLMNWFGVACELHRDWRNDVEGLGALFSSYLPNYRNLMTSYFTITKK"
     gene            complement(2522..3016)
                     /locus_tag="PKZ49_07190"
     CDS             complement(2522..3016)
                     /locus_tag="PKZ49_07190"
                     /inference="COORDINATES: protein motif:HMM:NF024751.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HND36385.1"
                     /translation="MFQETIKTCKAQNRVIVYIDESGFSHDMPRTHGYAPIGKRCHGV
                     CNWHARGRVNVIGALIGKCLLTVGLFISNIDADTFLGWTVHDLLPKLPPASVVVMDNA
                     TFHKRQDIQSVITQAGHTLEYLPAYSPDLNPIEYKWAQAKAIRKQQNQTVEQLFKIES
                     FYVA"
     gene            complement(3322..3702)
                     /locus_tag="PKZ49_07195"
     CDS             complement(3322..3702)
                     /locus_tag="PKZ49_07195"
                     /inference="COORDINATES: protein motif:HMM:NF024375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HND36386.1"
                     /translation="MTKSTTTYWNPLISEHRSQWDAIKGLEGIAEEITLSIDSTTGEY
                     TRLTRFLPGADTWALGGKTHPYPEEIFIVSGRLYDQAFGRWLEAGDYASRPPGELHGP
                     FKTDIGCVVLEISFPNRIAEGNND"
     gene            complement(3708..4346)
                     /locus_tag="PKZ49_07200"
     CDS             complement(3708..4346)
                     /locus_tag="PKZ49_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36387.1"
                     /translation="MSYVSLTHHQFDPNGFWEKPLQSVSVPAAQDLALFDQNGYDLTD
                     LEQHYAIINSAPAKSHREHHRALKTPWFTQPDRIEGAVLNHSLLFERKGYTGEALRQL
                     QQWAHTNPLIYKIIRIRPKWGLDFSMDYVDRSGNVFEVLHWEYDGFEYDEVAARKRLL
                     EVKFAAIDWDDAAINILRQKDQWHHLDFFAQSDWKCNYFGIVKERFKMVIWA"
     gene            complement(4408..4731)
                     /locus_tag="PKZ49_07205"
     CDS             complement(4408..4731)
                     /locus_tag="PKZ49_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
                     /protein_id="HND36388.1"
                     /translation="MNKPLKNQKPISPRQRKYWSQHATESSNALDLELGIFTWKDPHK
                     IALSLKCSADNSQRRKANPFASAMSMLNFYINRAGTHLPVTQREILESAKEELRKLYG
                     RFPSA"
     gene            complement(4802..5713)
                     /locus_tag="PKZ49_07210"
     CDS             complement(4802..5713)
                     /locus_tag="PKZ49_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HND36389.1"
                     /translation="MAGFMLTKQNNKLPILALLLGATCWGIAWYPYRLLNQSGFSGEA
                     ATALTYLVALISGIIFLRKNLSIQHNTASSENHWLIWIGITAGWTSIAYTLGVIHGEI
                     VRVLLLFYLAPFWTIFFARLLLNERLGLSGYWVIVLSLSGAMTILWQSGNDFPFPTQF
                     SDWMGLSGGIMFALNNVLIRKDQHHTIEQKSLAIWLGVFLLSFMLTGMTGTLPNFDQI
                     GNETGLILLGLGITIFLLSILLQYGLTHVPANQAIVILLLELFIAAIAAYYLADEQLS
                     TKEWIGGLMVVIASLLSTKIHYETSQA"
     gene            5846..7045
                     /gene="dapC"
                     /locus_tag="PKZ49_07215"
     CDS             5846..7045
                     /gene="dapC"
                     /locus_tag="PKZ49_07215"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HND36390.1"
                     /translation="MNPLLRNLQPYPFQKLSHLLEGITANASLAPINLHIGEPKHETP
                     AFILTELAKNLHGVANYPTTLGSKWLRTSIATWLVQRYQLTAIDPETEIIPVNGSREA
                     LFAFAQTVINSKTPEQPIVVCSNPFYQIYEGAALLAGAQPYFLNASAEQDFALNYEQL
                     PEAIWSRTQLIYVCSPNNPTGRVMTLDEWQHLFELSDRYGFVIASDECYSEIYFDEHT
                     PPLGALQAAQQLGRNKFTRLVVFSSLSKRSNVPGLRSGFVAGDAAILKDFLLYRTYHG
                     SAMNPAVQAASTIAWSDEQHVIENRRIYQEKFKDALAILNECMLAKMPDAGFYLWVKT
                     PINDRDFTQKLYRDYNVIVLPGSYLGREAHGFNPGENFVRIALVAPLAECSQAMQRIK
                     ACISQIH"
     gene            7073..7894
                     /gene="dapD"
                     /locus_tag="PKZ49_07220"
     CDS             7073..7894
                     /gene="dapD"
                     /locus_tag="PKZ49_07220"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HND36391.1"
                     /translation="MEQLQATIEAAFERRAELTPRNVEAGLKESIAQVIHLLDAGKLR
                     VAEKISGEWVTHQWIKKAVLLSFRIEDNNFIKGGFSNYFDKVPSKFADYSSRDFRDGG
                     FRVVPPAAVRKGAFIANNVVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPVQANPTIIEDNCFIGARSEIVEGVIVGENSVISMGVYIGQSTKIYDRE
                     TGQVSYGRIPPGSVVVSGSLPSENKQYSLYCAVIVKQVDAKTRSKTGINELLRGI"
     gene            7976..9193
                     /gene="fabF"
                     /locus_tag="PKZ49_07225"
     CDS             7976..9193
                     /gene="fabF"
                     /locus_tag="PKZ49_07225"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HND36392.1"
                     /translation="MRVVVTGMGVIAPIGTGVEQFWNAAVNGICGIRKISGFDASGQR
                     SQIAGEIIGFDPASVMSAKQIEQSDRFTQLALCAANFALKDAGDLDGYLPQRVAVSLG
                     SGMGGFSTFESSATRKFQKKSLPPFTVPRIMANSAAAWIATKYGFKGVNLTTSTACSS
                     GGNAIGVGLELIRSGKADVVIAGGAEACVLPLTMSGFEVLFALSTGFNNAPEQASRPF
                     DKGRDGFVIGEGAGIVVLEKEEHAKQRGALIHAYLSGYGCTCDAAHIVAPEIEGQVAA
                     MQAAIQDAQIQPEAVDYINAHATSTPLGDIVETKAIKQCFGSHAQKLSISATKSMIGH
                     SIGASAAIGSIATIMALKTGTIHPTINLVAPDPECDLDYTPNHAVRKPIQVGLCNAFG
                     FGGNNSTLVFTIN"
     gene            9212..9466
                     /locus_tag="PKZ49_07230"
     CDS             9212..9466
                     /locus_tag="PKZ49_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HND36393.1"
                     /translation="MTTADIEAKVIQFIASKVENVDASAVTAASQFKDLGLDSMDTVQ
                     LLFDAEEEFGVTFDGDEVKNLRTVGDIIDYINSHPPAAPQ"
     gene            complement(9508..>10649)
                     /locus_tag="PKZ49_07235"
     CDS             complement(9508..>10649)
                     /locus_tag="PKZ49_07235"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HND36394.1"
                     /translation="GHKAGDTVLTRTSGEISSIVRQTEIFSRLGGDEFAILSPLKSGD
                     DISALPSRIVNAIASIPIHFRDTNIRLTTSVGVAIFPIHGETAEDLIAHADAAMYQAK
                     NHGKNTWALYDPMRDSSEMMMNRLTWYNRIIQALEQNLFEIHFQGVYETTQSNLEHLE
                     ILVRMRDKNKPDQLIMPGQFIPVAEKNGQIVNIDRWVIKRSIELLHQNPNMPPVAINI
                     SGRTFDDPAIPNYISNLLNEYHVDPKRLIIELTETAAVSDVQDAQRFIEAVHQTGCRV
                     CLDDFGSGFSTFGYLKYLGVEVLKIDGLFIRDLPNNRDNQIFVKAMVEVARGLGKITV
                     AEFVEDAATLSMVKNLGVDLAQGYYLGRPEANFPRDHHKKAVNYL"
BASE COUNT         3042 a         2172 c         2419 g         3016 t
ORIGIN      
        1 cacggcgcgg cggtgcgctg gagatggttc agttatgggt aaatttgccc gccaagaata
       61 agatgtcacc gccacgttat caaagtattc tggatagcca gatccccgcc atcgatttgc
      121 cacaagatca aggaaaaata cgtgtcattg caggtgaatt cggtggtctt accggcccag
      181 cacgtacctt tacacccatc aacgtctggg acctgcgctt atccaatggg cagctgatcg
      241 accttgccgt gcctgatggt tataacacat tgctggcggt actcaaaggt tcgatgcgcg
      301 ttaataattc tgaaactatc aatacagcgg aggtcggcct gtttgatcaa acgggagacc
      361 atattcgcat cactagtaca tccgatacga cagcgttgtt gctgtgtggt aaaccgattg
      421 atgaacccat tgtcggtagc ggcccatttg tcatgaatac tacagaggaa atacgccaag
      481 ccatggcaga ttaccagagc gggaaaatgg gaaaaattac ataaatagca tgtatagcaa
      541 aaactacttg taaacacaaa ttggtttgac tattaagtaa ccatttttta gcaaaactaa
      601 cataggagaa taccatgagt cacatcaatc ccgacgaaac cctgatgctt cttatcgatc
      661 accaaagcgg tctatttcaa ctggtgaagg atattccagt gtcagacttg cgccgcaatg
      721 ccattgcact tgccaaagtt gctacaatac tcaaaatgcc ggtaattact tcggcatctg
      781 ttccggatgg cccgaacgga ccactgattc cggaaatcca tcaagccgct ccacatgcgc
      841 agtacgtggg aaggcgcggg gaaatcaatg tttgggatag ccctgatttt gtcaaagcag
      901 ttgaagctac tggccgcaaa acactgatcc ttgctggtac gctgaccagc gtatgcatgg
      961 cctttccttc catcagcgca gtggctgctg gttatagagt atatgctgta gtagatgcgt
     1021 caggaaattg gagccaaatg ggcacagaat taaccattgc tcgcattgtg caagcgggag
     1081 tgattccgat cgatactgtt gcagttattt cggagttgca aaaaacctgg aatcggccag
     1141 aagcaataga atttgctgat ctctacgctg attatcctat gttgcattac cggctgttga
     1201 tcgaaagcta tgacaaggca cagacggtgc agaaagcgaa gaattaagtt tgagagaatt
     1261 aaaatgactt tagaagaaaa attcaatgac tttaatcaga tgattctaga tggtgaaata
     1321 ttggaatcaa tggataagta ttaccattct gattgcatcg tgatcgaaaa aaatgatgtg
     1381 gtagcaacgg gtctgcaaca agctaaagcg cgcgaatccg agctatttga aggcgtcgag
     1441 gcatggctaa aatcggaaat tgtatcgact gcggtaggtg agaacgtgac aatgacagag
     1501 tggcattacg aatacaagca taaagacttt ggtcacaaga aattcgatca agtagcggta
     1561 cagcactgga agaatggcaa gatcatcagt gatcgctttt atgcgttgta ctgagcgatg
     1621 aatttacaat cagtagcata aggagaaaaa taatgaacaa aacttttaaa tataaccggc
     1681 tttcaaaaga tgatgcggtt ttattactgg tagaccatca agcaggattg atttctctgg
     1741 tgcaggattt ttcaccgaat gagttcaaaa acaatatatt ggctctgaca gcttgtggca
     1801 agtatttcaa gttgccaacc attctaacaa ctagtttcga agatggtccc aatggtccgc
     1861 tggttccgga aatcaaggaa atgcatccgg ctgcgcctta tattgcacgc cccggcaata
     1921 tcaatgcctg ggataacaaa gactttgtta acgcagtcaa aaagaccgac cgcaaacaat
     1981 taatcattgc cggcgtggtt accgaagtat gtgtcgcttt cccggcacta tccgcaatcg
     2041 aagaaggtta tgaagtcttc gtagttaccg atgcctccgg tactttcaat gaagtaacac
     2101 gcgatgcagc ctgggcgcgt atgcaatcgg ctggcgtaca attgatgaac tggtttggcg
     2161 tggcttgtga attgcatcga gattggcgta acgatgtgga gggtttaggt gccttgttct
     2221 ctagttatct acctaactat cggaatttaa tgacgagtta cttcaccatc actaagaagt
     2281 aagtaagcaa atttataacg ggagaccggt tttcatgaat gagcatgaga taaatttgca
     2341 ccggcgtctt atttttcagg cagtgtgggt gcatcttttt tacttttgaa aactggcacg
     2401 cttgcggcta atcttctgga gggtcttcaa tggataatct taaagcgcaa ctgattttat
     2461 gcaatggtca attcaccaca cttgattgct agaatcatcg agctatggct gctgctatag
     2521 cctacgccac ataaaatgat tcaattttaa agagctgctc aacggtttgg ttttgttgtt
     2581 ttctgattgc tttggcttgt gcccatttgt actcaatggg gttaaggtct ggtgaatacg
     2641 ctggtagata ttcaagcgta tgtccagctt gtgtgattac actttgaata tcctgccttt
     2701 tgtgaaaggt cgcgttatcc atgaccacaa ctgatgctgg tggaagtttt ggcaacagat
     2761 cgtgtaccgt ccagcccaaa aaagtatctg catcaatatt gctgatgaac aaccccaccg
     2821 tcagaagaca tttgccgatc aacgcgccga tcacattgac gcggcctctg gcatgccaat
     2881 tacacacacc gtgacaccgc ttgccaatgg gcgcataccc atgtgttcgt ggcatatcgt
     2941 gagaaaaacc actctcgtca atataaacga tgacgcgatt ttgagctttg cacgtcttaa
     3001 tcgtctcctg gaagatgtgc cgtttgtctt cgtccgcttt gggatgccgc agtgtttttt
     3061 ttatagctga tgttcatacg ccgcaacgcg tggccaatac ctttttcgct tacgcctaat
     3121 cgacgagctc gttctgcttg ataatcactt tatttgagcc ttgctatagg tggctggcgg
     3181 catggaagag gaacattgaa agacgagata tatgcaaaaa tatacattac agcaaatgta
     3241 ggcattgcga cgacaaacca aatatcaact cagttttagt ttggtttcta aatcaagcga
     3301 acaatttgag taaaaactgg atcagtcatt atttccttcg gctattctat tgggaaatga
     3361 gatttctagc acaacgcacc caatatcggt tttgaaagga ccatgaagtt ccccgggtgg
     3421 acggcttgca taatctcccg cttcaagcca gcgaccaaaa gcttgatcat ataaccggcc
     3481 actgacgata aatatttcct caggataggg atgtgttttt cctcccagag cccatgtatc
     3541 agcgccaggg agaaagcgag tgagtcgagt gtattcgccc gtagtagaat ctatactaag
     3601 cgtaatttct tcggcgattc cctccagccc tttgatggcg tcccattggc tacgatgctc
     3661 agaaatcagg ggattccaat aagtcgtggt cgattttgtc atagctttta tgcccaaatt
     3721 accattttga aacgctcttt tacgattccg aagtaattgc atttccagtc gctttgcgcg
     3781 aaaaaatcta agtggtgcca ctgatctttc tgtctcagaa tattaatggc agcatcatcc
     3841 caatcaattg ctgcaaattt gacttctaat agccgtttac gagcggctac ttcatcgtat
     3901 tcaaagccat catattccca atgcagaact tcaaagacat tgccagagcg atcaacgtaa
     3961 tccatgctga aatcgagtcc ccatttgggt cggatacgga taattttata aatgagcgga
     4021 ttagtgtgtg cccactgctg taattggcgt aacgcttcac cggtataacc tttacgttca
     4081 aagagtaggc tatgattgag tactgcacct tcgattcgat cgggttgtgt aaaccacggt
     4141 gtttttaacg cccggtgatg ttcgcggtgg gatttggctg gtgcagagtt gattatcgcg
     4201 tagtgctgtt ctaaatccgt tagatcgtag ccattttgat cgaataatgc caaatcttga
     4261 gcagctggta ctgacacgct ttggagaggt ttttcccaga aaccattagg atcaaattga
     4321 tgatgggtta aactgacgta gctcatggaa tactaaattt actcattgat tattgataaa
     4381 gttaattata aatagaaaaa ggtgctacta agcgctgggg aatcggccat ataatttccg
     4441 tagctcttct ttggcacttt ccaatatttc ccgctgcgtt actggtaaat gagttcctgc
     4501 tcggttgata taaaaattga gcatggacat cgctgaagca aatggatttg ctttgcgccg
     4561 ctggctatta tcagcggaac attttagtga taacgcgatt ttgtgtgggt ctttccaggt
     4621 aaatataccg agttcaagat ctaatgcatt acttgattcg gtggcatgtt gtgaccagta
     4681 tttccgttgc ctgggtgata ttggcttttg gttttttaga ggtttattca tattttcaaa
     4741 tacgaactcg acgaatttaa gcgatggttc tattgattaa aaaaagtaag actttccgta
     4801 attaagcctg ggatgtttca tagtgaattt tagtggaaag tagactagct attacgacca
     4861 tcaatccacc gatccattcc ttagtactta attgctcatc ggcaaggtaa taagctgcga
     4921 ttgcggcgat aaacaattca agcagcaaaa taacaattgc ttgattagcg ggaacatggg
     4981 ttaatccata ctgtagcaaa atactgagca agaagatggt tatgcctaac cccagtagga
     5041 tcaaccccgt ttcgttacca atttgatcaa aattgggtag cgttcctgtc atacccgtga
     5101 gcatgaagct cagtaagaag acccctagcc atattgccag ggatttttgt tcaatcgtat
     5161 ggtgttgatc tttacggatg agcacattgt tgagtgcaaa catgataccg cctgatagac
     5221 ccatccagtc actgaattga gtagggaatg gaaaatcatt acccgattgc caaagtatcg
     5281 tcatagcacc gctcaaggaa aggacaatga cccaataacc cgataaaccg agtcgctcat
     5341 ttaagagtaa tcgcgcaaaa aaaattgtcc agaacggggc taaataaaac aatagtaata
     5401 ctcttacaat ttcgccatga atcacaccaa gtgtataagc gatactagtc catccggcag
     5461 taatgccaat ccatatcaac caatgatttt cagatgaggc cgtgttgtgt tggatcgata
     5521 aatttttacg aagaaaaata atacccgaga tcaatgcgac taaataagtt aaagcagttg
     5581 cagcttcacc actgaatccg gattgattga gtaagcgata gggataccaa gcaatacccc
     5641 agcaagtagc acccaataat aatgcaagga taggtagctt attattttgt ttggttaaca
     5701 tgaatccagc catttataat gcggttctat agttaatagt taaaaaaatt tcaccgcagt
     5761 gaaaaataga agtcatgtag cctgaacaac taatattaat cgttaatgat ggcataaatt
     5821 caaggtttta gaattcatat cacaaatgaa cccgttattg agaaatcttc aaccctatcc
     5881 gttccagaaa ctgtcccact tacttgaggg tattacagct aatgcatctt tggcgccgat
     5941 aaatctgcat attggtgaac ccaagcatga aacgcctgca tttattctca ccgaattggc
     6001 caaaaatctt cacggtgttg caaattatcc tacaacactg ggttcaaaat ggctacgaac
     6061 cagcattgcg acatggctag tacaacgtta ccaattgaca gcaattgatc ctgaaactga
     6121 gatcattcct gtcaatggca gccgtgaagc gctatttgca tttgctcaaa ctgtcattaa
     6181 tagcaaaaca cctgaacaac ctatcgtagt ttgttctaat ccgttttatc aaatttatga
     6241 aggtgcagca ttacttgctg gcgcgcaacc ttattttctc aatgcatccg ctgagcaaga
     6301 ttttgcgctg aattatgaac agttaccaga ggcgatttgg tcacgtacgc aattaattta
     6361 tgtttgttcc cctaataatc caacgggacg ggtgatgact ttggacgagt ggcaacactt
     6421 attcgaattg tcagatcgct atgggttcgt gattgcttcg gatgaatgct attcagaaat
     6481 ttatttcgat gaacacacgc caccattagg tgcgctacag gcagcgcaac aattaggacg
     6541 caacaagttt acgcggttgg ttgttttttc cagtttatcc aaacgctcta atgttcctgg
     6601 cttacgctcc ggctttgttg caggggacgc agcaattttg aaagattttt tactgtatcg
     6661 gacttatcat gggagcgcca tgaatccagc agttcaagca gccagtacca ttgcctggag
     6721 cgatgaacag cacgtgatag agaaccgccg gatttatcag gagaaattta aagatgcgct
     6781 cgcgatttta aatgaatgta tgctggcaaa gatgcccgat gcgggtttct atttatgggt
     6841 caaaacgcct atcaatgatc gggatttcac gcaaaagtta tatcgcgatt ataatgtaat
     6901 cgttttgcca gggagctatc ttgggcgtga agcccatgga ttcaacccag gagaaaattt
     6961 tgttcgtatt gcgctcgttg ctccattagc tgaatgctca caagcgatgc agcgaataaa
     7021 agcatgcatt tcccaaattc attaaaatta ttaaattcat tcaaggaata acatggaaca
     7081 gttgcaagca acgattgaag cagcgtttga acggcgtgcc gaattaacac cccgtaatgt
     7141 ggaggcgggt ctgaaagaat caatcgctca agtcattcat ttactcgatg caggtaaatt
     7201 acgagttgca gaaaagatta gtggtgaatg ggtaacgcac caatggatca agaaagcggt
     7261 gctcctatct ttcaggattg aggataataa ttttattaaa gggggattct cgaattattt
     7321 tgataaggtt ccctcaaaat ttgctgatta tagttcccgt gattttcgcg atggtgggtt
     7381 tcgtgtcgtt cctcctgcag ctgttcggaa aggtgctttt attgcgaata atgtggtgtt
     7441 gatgccttct tatgtcaata taggtgctta tgtcgatgaa ggaacgatgg ttgatacttg
     7501 ggcgacagta gggtcttgtg cccaaattgg taagaatgtc catttatcgg gaggcgttgg
     7561 gattggcgga gtacttgaac cggtacaagc aaacccgacc atcattgaag ataattgctt
     7621 tattggtgcc cgatctgaaa ttgtcgaagg tgttatcgtt ggtgagaatt ccgtgatctc
     7681 aatgggggtt tatattgggc aaagtactaa aatctatgac cgggaaacgg ggcaagttag
     7741 ctatgggcgt attccacctg gatcagtcgt cgtgtcagga agtttacctt cagagaataa
     7801 gcaatatagt ctctattgtg cggtcattgt aaaacaagtc gatgccaaga ctcgctctaa
     7861 gactggaatt aatgagttgc tacgtggaat ttaacgactc gtcggacttc tacttaacgt
     7921 tagcgaataa cagaatgttt tgagcaatcg attttcaatt actggatgat tagaaatgcg
     7981 tgtcgttgtg actggaatgg gggttattgc tccgatcggg acaggagttg aacaattttg
     8041 gaatgctgca gtaaatggaa tctgtgggat acgcaaaatt agtggctttg atgcatctgg
     8101 ccaacgttcc caaattgccg gtgaaatcat tgggtttgac cctgcttccg tgatgtcagc
     8161 caaacaaatt gaacaatctg atcgatttac acagctagcg ttatgtgccg ctaattttgc
     8221 gcttaaagat gcgggagatc tcgatggtta tttacctcag cgcgtagcag tgagcttagg
     8281 ttcggggatg ggtgggtttt caacgtttga atcttcagcg acacgaaaat ttcagaaaaa
     8341 atcattaccc ccttttacgg ttccacggat catggccaat tctgcagcag cctggattgc
     8401 gaccaagtac ggatttaaag gtgttaacct gacgacgagt acagcgtgct catcaggcgg
     8461 taatgcgatt ggggtggggc ttgaattgat tcgctctgga aaagcagatg ttgttatcgc
     8521 aggaggtgct gaagcatgcg tattaccttt gacgatgtct ggttttgaag tgttatttgc
     8581 gttgtctact ggatttaata atgccccaga acaggcatca agaccctttg acaaaggtcg
     8641 agatggcttt gtaattggtg aaggcgcagg tatcgttgtt ctagaaaaag aagaacatgc
     8701 caaacaacgt ggagccttaa ttcatgcata tctatccggt tatggttgta cttgcgatgc
     8761 agcacatatc gtggcgccgg aaatagaggg tcaagttgcc gcgatgcaag cagccataca
     8821 agatgcacag atccaacctg aagcggttga ttatattaat gcgcatgcaa cttctacacc
     8881 actcggcgat atcgtagaga ctaaagctat taaacagtgc tttggatccc atgcgcagaa
     8941 attatccatt agtgcgacta aatcaatgat tggacactct atcggtgcgt cggcagcaat
     9001 tgggagcatt gccacgatca tggcactaaa aactggcact attcatccca cgatcaattt
     9061 agtggcgccc gatccagaat gcgatcttga ctatacaccc aatcacgcag ttagaaagcc
     9121 gatccaggtt gggttatgta acgcttttgg ctttggtggt aataattcca cacttgtttt
     9181 tacaataaat taaatcatag gaatacatca tatgactaca gcagatatcg aagcaaaagt
     9241 cattcagttc attgcttcta aagttgagaa tgtcgatgct tcagcggtta cagcagcatc
     9301 acaatttaaa gatttaggtt tggactcaat ggatacagtt cagctattat ttgatgcgga
     9361 agaagaattt ggtgtcacat ttgatggcga tgaagtgaag aatctccgaa cggtagggga
     9421 tattatcgat tatattaaca gccatcctcc agcagcacct caataaagct gagtgggtgg
     9481 attgctaaac atggtttagt aaattattta gagataattg accgcttttt tatgatgatc
     9541 tctagggaaa ttggcttctg ggcgacctaa gtaataaccc tgtgcaaggt caaccccaag
     9601 attcttgacc atgctcaagg ttgcagcatc ttcaacaaat tcagccacag tgattttacc
     9661 taatccacgt gctacctcca ccatggcttt gacaaatatt tgattatcac gattattcgg
     9721 aagatcacga ataaataagc catcgatttt taatacttcc acaccaagat atttgagata
     9781 accaaaggta gaaaacccac tcccaaaatc atccaagcaa acacggcaac ctgtttgatg
     9841 aactgcctca atgaaacgtt gtgcatcttg gacgtcagaa actgcagccg tttctgttaa
     9901 ctcgataatc aaacgtttgg gatcgacatg atattcattt aatagattac taatgtaatt
     9961 cggtatagca ggatcatcaa aggttcgacc agaaatattg attgcaacgg gcggcatatt
    10021 ggggttttgg tggagcaact caatactacg ttttattacc caacgatcaa tattgacgat
    10081 ttgcccattt ttctcagcaa ctgggataaa ttgtcccggc ataatgagct gatcaggttt
    10141 atttttatcg cgcattctaa cgagaatttc taggtgttct aaattactct gggttgtttc
    10201 gtaaacacct tgaaaatgaa tttcaaacaa gttttgttca agcgcttgta tgattcggtt
    10261 ataccacgtt aaccgattca tcatcatttc tgacgaatca cgcattggat catagagtgc
    10321 ccaggtgttt tttccatgat ttttagcctg atacattgcg gcatctgcgt gggcaatcaa
    10381 gtcttcagcc gtttctccat ggatgggaaa gattgcgact ccaacgctgg tggtgaggcg
    10441 tatattcgta tcacgaaaat gaattgggat agaagcaata gcattgacga tgcgagaagg
    10501 taatgcgctg atatcatcgc ctgattttag tggactcaaa atcgcgaatt catccccccc
    10561 gagtcgtgaa aaaatttccg tttgtcgaac gatgctagaa atttccccag acgtacgagt
    10621 taaaacggta tctccggctt tatggccaa
//
LOCUS       DAOQKQ010000090        10522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_2966_length_10522_cov_5.358364, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000090 DAOQKQ010000000
VERSION     DAOQKQ010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 10522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10522
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2966_length_10522_cov_5.358364"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /gene="folE2"
                     /locus_tag="PKZ49_07240"
     CDS             <1..679
                     /gene="folE2"
                     /locus_tag="PKZ49_07240"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HND36395.1"
                     /translation="QHTVAMFNMYVNLPHNFKGTHMSRFVEILNSHEREISVESFEDI
                     LRAMVTRLESDSGHIEMTFPYFVNKSAPISGVQSLLDYEVTFIGEVKQGEYRFTMKVV
                     VPVTSLCPCSKKISDYGAHNQRSHVTVAVRTNSFVWIEDVIKIAEEQASCELYGLLKR
                     PDEKYVTERAYDNPKFVEDIVRDVAEVLNHDDRIDAYVVESENFESIHNHSAYALIER
                     DKHRKQI"
     gene            complement(689..2227)
                     /gene="murJ"
                     /locus_tag="PKZ49_07245"
     CDS             complement(689..2227)
                     /gene="murJ"
                     /locus_tag="PKZ49_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HND36396.1"
                     /translation="MNLLKALATVSSMTLISRILGFVRDVLIARIFGAGVATDAFFVA
                     FRIPNLLRRLFAEGAFSQAFVPILAEYKNNRSEAETRALIDHVTTLLGVTLFVVTLIG
                     IVAAPLIIYISAPGFSGDIEKFSLTVTLLRITFPYILFISLVALAGGILNTYSQFSVP
                     AFTPVLLNLSFIGCSLWVVPYVDPPVLALAWAVFLGGILQLLFQIPYLIRIKRMPRIR
                     FKTKDTGAGRVLKLMLPAIFGVSIGQISLLINTIFASLLITGSVSWLYYADRLMEFPA
                     GLLGVALGTVLLPSLSRHYASHSNEEYSRLLDWGLRLTVLLTLPAAVALALLATPLIT
                     TLFYYGAFTAEDVWMTRQALIAYSFGLLGLIMVKVLAPGFYARQNIKTPVKIAVLTLA
                     ATQVMNLIFIIPLQHAGLALAIGLGACLNAGLLYYKLRTNDIYQPLPGWLKFMVKILV
                     AVIVMGTTLWLSSGNDSLWLQMNAIDRALRLTFTVCIGAIAYFITLWLLGFRLKDFSQ
                     LGTS"
     gene            2554..3207
                     /locus_tag="PKZ49_07250"
     CDS             2554..3207
                     /locus_tag="PKZ49_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HND36397.1"
                     /translation="MGIDSSLVHCRRCSRLASFLDAVKAQYPTYHARPVAAFGDINPK
                     LLIVGLAPGMHGANRTGRPFTGDYAGILLYQTLFKFGLANQNESISSTDALCLLHTRI
                     TNAVKCLPPENKPKPCEIKQCNDYLSNEIIHFIEQNGKGILALGNVAHQAVLMAFNLK
                     LKHYPFSHGAIYVLPNELKLYDSYHCSRYNTQTKRLTQEMFEQVFSEICADLAIPNH"
     gene            3224..3409
                     /locus_tag="PKZ49_07255"
     CDS             3224..3409
                     /locus_tag="PKZ49_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HND36398.1"
                     /translation="MPYVNIRVAGTLTREQKQKIAEEITDTLERIGNKPKSYTYISFD
                     EMPHDTWAIAGKLLGDD"
     gene            3429..5246
                     /gene="uvrC"
                     /locus_tag="PKZ49_07260"
     CDS             3429..5246
                     /gene="uvrC"
                     /locus_tag="PKZ49_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HND36399.1"
                     /translation="MSEVAFDGKVFAAALPNLPGVYRMLNAAGEVIYVGKAIDLRKRV
                     SSYFQKSMLGPRIQLMVSQITGIETTVTRSEAEALLLENNLIKSLNPRYNILFRDDKS
                     YPYVKLSQHPFPRLAFYRGTIDHRHQYFGPFPNAGVVRESIQLLQKVFRLRTCEDSVF
                     HHRTRPCLLYQIKRCSGPCVNLIEQSDYQADVKSASLFLQGKQNEVIRNIADKMQQAS
                     NQMDYEQAAVFRDQMQALRKIQEKQFVDSGKSLDADVIAGVKASDGSEKVCVNLVMIR
                     GGLHLGDKSFFPQNIQGYEMVDVLEAFIAQHYLNRSIPPIIIIDTKIQTKILQSLLTE
                     QANRKVTIISNSIGERRMWLDMATANADLALKQQLQRQASQEDRLQILQQALNLSNLN
                     RIECFDISHTAGEATVASCVVYDHFAMQNNEYRRYNITGVEPGDDYAAMKQALHRRYA
                     KVAEGQGKIPDLILIDGGKGQVSVAQEILTELGLADIPFLGVAKGEDRKPGLEQLVFP
                     SGEKPLKLPADHPGLHLIQQIRDEAHRFAIQGHRARRNKARTVSTLEEIAGIGAKRRQ
                     SLLGRFGGLKGVLTASVEELQQTEGISQALAERIYRELH"
     gene            5320..5880
                     /gene="pgsA"
                     /locus_tag="PKZ49_07265"
     CDS             5320..5880
                     /gene="pgsA"
                     /locus_tag="PKZ49_07265"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HND36400.1"
                     /translation="MPFNLPNLLTWLRIFAIPLFVGIFYIPGLSPFGQNLVATIIFSV
                     AAITDWLDGYLARTLNQTSAFGAFLDPVADKLMVAAALIILLHLERLDAPIALIIIGR
                     EITVSALREWMAQIGKAKSVAVSFMGKIKTTAQMIAIPLLLYHETIGESFNSHLVGTW
                     FIYLAAFLTLWSMIYYLKAAIPHALK"
     gene            complement(5961..6572)
                     /locus_tag="PKZ49_07270"
     CDS             complement(5961..6572)
                     /locus_tag="PKZ49_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36401.1"
                     /translation="MFTKIFKGLGLSLLVTGVLVGCTTTQKITSSARTTTEQLLISEA
                     LKRSLPKQTNAPLPIPQGSVVKLDVIGLTGDKDIVKGAVAGWLGQHGYIAQDEPATYR
                     MNIMVDSIGTEQANTFFGIPPISAALIPISLPKLAFYEAEYQTGYSRIHLDIFEVPSG
                     RFVGSTSSFIGDTYFNAHTILFLFKLKRTDLTSPPEVGTFMGH"
     gene            6766..7929
                     /gene="dinB"
                     /locus_tag="PKZ49_07275"
     CDS             6766..7929
                     /gene="dinB"
                     /locus_tag="PKZ49_07275"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HND36402.1"
                     /translation="MNQSSRRIAHLDMDAFYASVELLRYPQLKGLPVVIAGRAEHKPQ
                     IQADGQLQFSRLSDYIGRGVITTSTYEARALGIYSAMGVMKAAQLAPQAILLPVDFTA
                     YRHYSRLFKAAVVEIAPEIEDSGIDEIYIDLTTLADDNHTLAKRIKQAVLNATGLTCS
                     IAITPNKLLAKIGSDLNKPDGVTILSAEDIPTRIWPLPARKINGIGPKAEKKLIALGI
                     TTIGQLAEAELHWLQIHFSHNYAIWLYEAARGIDDRPVLTCSEPKSVSRETTFERDLH
                     PIRDRSILTAELIALCKRVANDLNRKAYQGRTIGIKIRFEDFNTITRDITLGKSVIDA
                     TAILSATRNCLRRVKLDKKIRLLGVRVSSLVALAEMQPLEDLHQVELPLSSNR"
     gene            complement(7996..8391)
                     /locus_tag="PKZ49_07280"
     CDS             complement(7996..8391)
                     /locus_tag="PKZ49_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36403.1"
                     /translation="MKIALFKVSTIAATTLAVMALSLPASSAEAADIRVQCEKRSNRS
                     KVSVDGNNLISGNYSAKITSGANTKVSAYKPSVGDEIEFDFDSDRNDVAAGAIRISSS
                     FIQNGQVTGELINEKGFTVASSTSACRIK"
     gene            8575..9054
                     /locus_tag="PKZ49_07285"
     CDS             8575..9054
                     /locus_tag="PKZ49_07285"
                     /inference="COORDINATES: protein motif:HMM:NF013981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HND36404.1"
                     /translation="MVEITLLIWLICVNGAPILLAKILGKRFNIPIDGNRLWRDGQPL
                     FGQSKTIRGILVALLVSIGAGSLLQLPIVMSITFGLLSMVGDLTSSFVKRRLGYKPSA
                     MALGIDQIPESLFPLLGIQFWLTLSFSQIVGIVLAFFCLELLLSRILFWMKIRKKPY"
     gene            complement(9051..9986)
                     /locus_tag="PKZ49_07290"
     CDS             complement(9051..9986)
                     /locus_tag="PKZ49_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HND36405.1"
                     /translation="MDTLEQQLSERLGRIHARQRLGIESDHKQQVFGKGLNFFHPENW
                     YATHTLIRFAIQLVGLYGRGQRNARNLQVRENSFYLAHLPEAFHQFRILHLTDLHVDM
                     DDRNLTALIETIIDLPYDICVLTGDYRAQTFGTIDHAIAGMQRLIAMLKQPIYGVLGN
                     HDSIRMLPALESMGVIMLMNEAVKLEKDGESIFLAGVDDPHYFRVDNLEKAAQTIPHD
                     AVSLLLSHTPEIYRQAAHADFDMMLCGHTHGGQICLPGGIPVTLDSSCPRYLGAGKWQ
                     YRTMQGYTSVGAGTSIVNARFNCPPEVTIHQLLKN"
     gene            10328..>10522
                     /locus_tag="PKZ49_07295"
     CDS             10328..>10522
                     /locus_tag="PKZ49_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36406.1"
                     /translation="MSMIQQTKSELLEEVRQLRAELAAVKWMTEKRAQELYMSEERFS
                     LAMRGANDGLWDWNLETDEIY"
BASE COUNT         3188 a         2013 c         2282 g         3039 t
ORIGIN      
        1 ccagcatacg gtagcaatgt ttaacatgta tgttaattta cctcacaatt ttaaaggcac
       61 tcatatgtca cgtttcgtgg aaatactgaa tagccatgaa cgcgaaatat cggtggaatc
      121 gtttgaagat attctaaggg cgatggttac tcgactcgag tccgattcag gtcatattga
      181 gatgaccttt ccatactttg ttaataagtc ggctccgata tccggtgtac aaagtctact
      241 cgattatgaa gttacattca ttggcgaggt aaagcaagga gagtatcgtt ttacaatgaa
      301 agtcgtggtg ccagttacga gtctttgtcc ttgttcaaaa aagatttctg attatggtgc
      361 tcataatcaa cgttcgcacg ttactgttgc agttcgcacg aatagttttg tttggattga
      421 agatgttatt aaaattgcag aagagcaagc ttcttgcgag ttatatgggt tattgaaacg
      481 ccccgatgaa aaatatgtta cggaaagagc gtatgataac cctaaatttg ttgaagacat
      541 cgttcgggat gtagctgaag tactcaatca cgatgatcga atcgatgctt atgtggttga
      601 atctgaaaat tttgaatcca tacacaacca ctcggcttat gctttaattg aacgagataa
      661 acatcggaaa caaatataag tttgataatt aactagtacc gagttgagaa aaatccttta
      721 agcgaaaccc taagagccac agtgttataa agtaagcaat tgccccaata caaaccgtga
      781 aagttaaacg gagtgcacgg tcgatggcat tcatttgtaa ccataagctg tcatttccac
      841 tactcaacca aagggtggtg cccataacaa tcacggcaac tagaatttta accatgaatt
      901 taagccaacc aggtaagggt tgatagatat cgttagtccg cagcttatag taaagtagtc
      961 ctgcattaag gcaagcacct aaaccaatcg ctaaagctaa gccagcatgc tgcaacggga
     1021 tgataaatat taaattcatg acttgtgtgg cagcgagtgt taatacagca attttgactg
     1081 gcgttttgat attctgtcgc gcatagaagc caggtgcgag tactttaacc ataatgagtc
     1141 ccagtaatcc aaagctatat gcaatgagcg cttgccgtgt catccaaaca tcttccgctg
     1201 taaatgcacc ataataaaac agtgtggtga ttagtggggt tgcaagcaat gctaatgcca
     1261 cagcagcagg taaagtcaat aaaaccgtta aacgtaatcc ccagtccagc agacgcgagt
     1321 attcttcgtt actgtgactc gcataatgcc gagataacga aggtagcaaa accgttccaa
     1381 gtgcgacacc taaaagtccc gcaggaaatt ccattaaccg atctgcgtaa taaagccacg
     1441 aaacgcttcc tgtgatgagt aaagaagcaa aaatcgtgtt gattaatagg ctgatttgcc
     1501 caatcgatac gccaaaaata gcaggtaaca ttagcttaag cactcgccct gcgccggtat
     1561 ccttggtttt aaatcgtatg cgcggcatgc gcttaattcg aattaaataa ggaatttgga
     1621 atagaagctg taaaatcccc cctaaaaaaa cagcccatgc gagtgcaagt acaggaggat
     1681 caacataagg aacgacccat aatgagcagc cgatgaatga taaattcaat aagactgggg
     1741 taaatgcggg tactgaaaat tgactgtagg tatttaggat gccaccggcc agtgcaacca
     1801 atgaaataaa taaaatatag gggaaagtga tgcgcaaaag ggtgacagtg agcgagaatt
     1861 tttcaatatc gccagagaat ccaggagcgc tgatataaat aatcaaggga gctgctacga
     1921 taccaatgag tgtaacaaca aacagtgtaa cgcctaataa tgttgtcaca tgatcgatta
     1981 atgctcgcgt ttcagcttca ctccggttat ttttatattc ggctagaatg ggaacaaaag
     2041 cttgtgagaa agctccttcc gcaaataatc tgcgcaataa gttagggatt cggaacgcaa
     2101 caaaaaaagc atctgtagcg acacccgcac caaaaattct cgcgatcaat acatcgcgta
     2161 caaatcctag aatacgggaa atcaatgtca tgctactaac ggtagccagt gctttgagta
     2221 gattcaattt atttgggaaa gaagcctaac cacaaattgg tgagcgcata ttatgtttga
     2281 aaagagccta ttttacgtgg gtttaagaaa ttgtgatcaa taaatattgt tagtttgggt
     2341 aaatgaatga agctcttttt ttcaatctgt gcagattgat aataaaatat caaatatcaa
     2401 ttaatttaag aatggaatga aaggaaatcg tttatgttga aaaaagttgc cattgtcgtc
     2461 gcattagtat ttgctgttgg ttatatttat ctgatggtta agtttttcta aatctaagaa
     2521 gtgtaattta ggattaatct aaattttcta tcgatgggaa ttgattcgtc gttggttcat
     2581 tgtcgacgct gtagccgtct tgcttcattc ttagatgctg taaaagcaca atacccaact
     2641 tatcatgcaa gaccagtcgc agcatttgga gatatcaatc cgaaactgtt gatcgttggt
     2701 cttgcgccag ggatgcatgg cgcaaatcgc actgggaggc cttttacagg agattatgca
     2761 ggcattttgc tttaccaaac gttattcaaa tttggtttgg caaatcagaa tgaatcgatt
     2821 tcgagcactg acgcattgtg cttgcttcat acgcgtatta cgaatgcagt aaaatgtctg
     2881 ccacctgaaa ataaacctaa gccctgtgaa atcaagcaat gcaatgatta tttatcgaat
     2941 gaaataatac atttcattga acagaacggc aaagggatac tggcgctggg gaatgtggct
     3001 caccaggccg tgttgatggc gtttaattta aaattaaagc actacccatt tagccatggg
     3061 gcaatttatg ttttacccaa tgaactcaag ctttatgaca gttatcattg tagtcgctac
     3121 aatactcaaa ccaagcgcct gactcaggag atgttcgaac aggtgttttc agaaatttgt
     3181 gccgacttag ctattcccaa tcattaatct taggagaaat cgaatgcctt atgtgaatat
     3241 tcgtgttgca ggtacattga ctcgtgaaca gaaacaaaaa attgctgaag aaattacaga
     3301 tacactcgaa cgaatcggta ataagcccaa atcgtatacg tatatttctt ttgatgaaat
     3361 gcctcatgat acgtgggcta tcgctggtaa attgttgggt gacgattgat taataagcat
     3421 taaacatcgt gtctgaagtt gcatttgatg gtaaggtttt tgctgcggca ttaccaaatt
     3481 taccaggcgt atatcgaatg ctgaatgcag caggtgaggt catctatgtg gggaaggcaa
     3541 tcgatcttcg caaacgggtt tcatcgtatt ttcagaaatc gatgctaggg ccacgtattc
     3601 agttaatggt gtcacaaatt acaggaattg agacaacggt gacacgttca gaagctgaag
     3661 cactgttact tgagaataat ctgattaaaa gtttaaaccc tcgatataac attttgtttc
     3721 gggatgataa atcgtatcct tatgtaaagc taagtcagca tccttttcct cgtttggctt
     3781 tttatcgggg cacaattgat catcgccacc agtacttcgg tccttttccc aatgctgggg
     3841 ttgtacgtga aagtatccaa ttgctgcaaa aggtttttcg gttaaggacc tgtgaggata
     3901 gtgttttcca tcatcgaact cgaccttgct tactctatca aataaagcgc tgtagtgggc
     3961 cctgtgttaa cttgatcgaa caatctgatt atcaagcaga tgttaaaagt gcttcgcttt
     4021 ttttgcaggg taaacagaac gaagtaatta gaaatattgc agataagatg cagcaggcat
     4081 caaatcagat ggactatgag caagcagctg tctttcgcga tcaaatgcag gcgttacgga
     4141 aaatccaaga aaagcaattt gtcgatagcg ggaaatcctt agacgctgat gtgattgcag
     4201 gtgtcaaagc atccgatgga tcagagaagg tctgtgtcaa cttggtaatg attagaggtg
     4261 gtttacactt aggagataaa agctttttcc cacaaaatat acaaggctac gaaatggttg
     4321 atgtgcttga agcatttatt gcgcagcact atttaaaccg aagtattcct cctatcatca
     4381 tcattgatac taaaattcaa acgaaaatac tgcaatcgtt gttgactgag caagcgaacc
     4441 ggaaagttac cattattagc aactcgattg gggaacgtcg tatgtggctg gacatggcta
     4501 cagccaatgc tgatctagcg ctaaaacaac aattgcagcg gcaagcgagt caagaagacc
     4561 ggttgcaaat tttgcagcag gctttaaatt tatcaaatct taatcggatt gagtgctttg
     4621 atattagtca tactgcagga gaagctacgg ttgcttcctg cgttgtttat gatcattttg
     4681 ccatgcaaaa taatgaatat cgccgctata acattacggg tgtcgaaccg ggtgacgatt
     4741 atgctgcaat gaagcaagcg ttgcaccggc gttatgctaa agttgcagaa ggtcagggta
     4801 aaattccaga cttgattcta atcgatggcg gtaaaggtca agtcagtgta gcacaggaaa
     4861 ttttgactga attggggcta gcagatattc cttttctagg cgtggcaaaa ggagaagatc
     4921 gtaaaccagg gcttgaacaa ttagtttttc catcaggaga aaaaccgtta aaattaccag
     4981 ccgatcatcc tggtttgcat ctgattcagc aaattcgcga tgaagcgcat cgttttgcga
     5041 ttcaaggtca ccgtgcgaga agaaataaag caagaaccgt ctcaacactc gaagagatcg
     5101 caggcattgg tgctaaacgt cgccagagtt tattggggcg ttttggggga ttgaaaggcg
     5161 tgttaacagc gagcgttgaa gaattacagc aaacagaagg catcagtcaa gcattggcag
     5221 aacgtattta tcgggaatta cattgacatt acactgcgca tccgtacatt cattttcaat
     5281 gcatctcact agtcattact ttttcttact gattatccaa tgccctttaa tttacctaac
     5341 cttctaacct ggcttaggat tttcgcaatt ccgttatttg tcgggatttt ttatattcct
     5401 ggattatcgc cttttggcca gaatttagtg gcgaccatta tttttagtgt agcagccatt
     5461 accgattggt tagatggtta tctcgcacgg acactcaatc aaacttcagc ttttggtgct
     5521 tttcttgatc ctgtagcaga taaattaatg gtggcagcgg ccctcattat tcttttgcat
     5581 ttagaacgac tcgatgcacc cattgctttg atcattattg gtcgtgaaat tactgtttcg
     5641 gcacttcgtg aatggatggc tcaaatcggt aaagcaaaga gtgtagccgt ttcatttatg
     5701 ggtaaaatca aaacgactgc acaaatgatt gccattccat tgctgctata ccatgaaacg
     5761 attggagaat catttaattc tcatctcgtg ggtacttggt ttatttacct tgctgcgttt
     5821 ctgacgctgt ggtcgatgat ttattatttg aaagcagcta ttccacatgc actcaaataa
     5881 tgtgctgaaa agttttaagt tttttgtgct ttaggaaaag attgtttttc taaagcacag
     5941 gggtttggat taggctggat ttagtgtccc atgaaggtac caacttctgg tggtgaagtt
     6001 aggtctgttc tcttgagttt aaatagaaac agtatcgtat gggcattaaa ataggtatca
     6061 ccaataaagg aactggttga tccaacgaat cgaccggacg gtacttcaaa aatatcgaga
     6121 tggattctgg aataacccgt ttgatactca gcttcataga aagcgagctt tggcaatgaa
     6181 atcggaatca gcgccgcaga gatcgggggg ataccaaaga acgtattggc ttgttcggtt
     6241 ccaatcgaat ctaccatgat attcatgcga tacgttgctg gttcgtcttg agcgatgtaa
     6301 ccatgctgcc caagccatcc agcgacagca cctttgacta tgtctttatc accagtcagt
     6361 cctatcacat cgagttttac aactgaacct tgtggaatcg gtaatggtgc attagtttgt
     6421 ttgggaaggc tacgttttaa ggcttcactg attaatagct gctcagtcgt tgttcttgct
     6481 gaactggtta ttttttgggt tgtagtacaa ccgaccaaaa cgcccgtaac taataagctt
     6541 agacctaatc ctttgaagat tttcgtgaac atgaagcaac tccttgttgt gatgaatgag
     6601 tgttttgatc gacattaatt agaacgaggg cacattaaca taaattccta ataggaaaaa
     6661 ccctgtcaaa attaagttta tgtaagttgc taagcatgat ttcttattca tcacgataaa
     6721 ataaactttt agtaatttta cttacaacta atcaatttaa ttggagtgaa tcaatcatct
     6781 cgacgcattg ctcatctcga tatggatgca ttctatgcat ccgttgaact gttgcgttat
     6841 ccgcagttaa aaggattgcc tgttgtgatt gcgggtcggg cggagcataa acctcaaatt
     6901 caagcagatg gtcaattaca attttcgcgt ttgagcgatt acataggacg tggtgtcatt
     6961 actacttcga cgtatgaagc tcgcgcattg ggtatttatt ctgctatggg tgtcatgaaa
     7021 gctgcacaat tagcacctca agcaatttta ttaccagtag attttaccgc atatcgccac
     7081 tattcgagat tattcaaagc agctgtcgtt gaaattgcac ctgaaattga agattctggc
     7141 attgatgaga tttatattga tttaaccacc ctagcagacg ataaccatac acttgcaaaa
     7201 cgtattaaac aagcagtgct gaatgcaacc ggtttgactt gctcgatcgc gattacgccg
     7261 aacaaattac ttgccaagat tggatcagat ctcaataaac ccgatggcgt gacaatattg
     7321 agtgcagaag atattccaac tcgtatttgg ccacttccag ctcgtaagat aaatggtatc
     7381 ggcccgaaag cggagaaaaa acttattgca ctgggaataa ccactattgg tcaattagcc
     7441 gaagcagagt tgcactggct acaaatccac tttagccata attacgcaat ttggttgtat
     7501 gaagctgccc gagggatcga tgaccgtcct gtcttaacgt gttctgaacc caaatcagtg
     7561 agtcgtgaaa cgacatttga gcgagattta cacccgattc gtgatcgatc aattttgaca
     7621 gcagaattga ttgccctttg taaacgcgtt gccaatgatc ttaatcgtaa ggcctatcaa
     7681 ggaaggacga ttggcatcaa aattcgtttt gaggatttta atacgatcac tcgtgatatt
     7741 acgctaggaa agtcagtgat tgatgctact gcgattttat cagcaacccg taactgctta
     7801 cgccgtgtca agttagataa aaagatacgt ttacttggcg tacgggttag ctccttagtc
     7861 gcacttgcag aaatgcaacc tttggaagat ctgcatcagg ttgaattacc tttatccagc
     7921 aatcgataag ttgaaaaaaa agccctgaaa aaatagaggg gaaatttcag ggcccaaaaa
     7981 gtaacaaatt aatcgttatt taatgcggca tgcgctggta ctagatgcaa ccgtgaatcc
     8041 tttctcatta atcaattcac ccgttacttg cccgttctga ataaagctgc ttgaaatacg
     8101 aatggcacca gcagcaacgt cattacggtc actatcaaaa tcaaattcaa tttcatcacc
     8161 gactgagggt ttgtaagcag atactttggt atttgcgccc gaagtgattt ttgcactgta
     8221 gttacctgaa atcaggttgt ttccatcaac agaaacttta gagcggttgg aacgtttttc
     8281 gcattgaacg cggatatcgg cagcttcggc actactggca ggtagcgata gcgccattac
     8341 tgctaatgtg gttgcagcaa tggttgatac tttaaacagg gcgattttca ttttttgact
     8401 cctttaaatt atttaacagt gagatttatg caataaacaa tcaataaacg cggatatatt
     8461 tcccacgctt ttttcagtat atgtgtgaaa aaatcccatg tcaacaaaat ttttgtcaaa
     8521 aataaatgtg gaaatctaaa atgactgaca acattcaaag gtaaagaaaa gataatggtg
     8581 gaaattacat tgctgatttg gttgatctgt gtgaatgggg cgcccatttt gttagcaaaa
     8641 atattgggaa agcgcttcaa tataccaatc gatggaaatc gcttgtggcg tgatggtcaa
     8701 ccgctattcg gtcaatccaa gacaattcgg gggattttgg ttgcgctact ggtatcgatc
     8761 ggggcgggaa gtttattgca attacctatc gtaatgagta tcacgttcgg attgttatcg
     8821 atggttggtg atctaacttc cagttttgtc aaacggcgat taggatacaa accgagcgca
     8881 atggcgctag gtatcgatca aattcctgaa tccttgtttc cattactggg aatccagttt
     8941 tggctaacac ttagcttttc acaaattgtt gggattgtgc tggcgttttt ctgtctagag
     9001 ttgttgctgt cacgtatttt attctggatg aaaatacgta aaaaacctta ttagtttttt
     9061 aacaattgat gaatagtgac ttccgggggg caattaaaac gagcattgac aatcgaagtg
     9121 cctgcaccca ctgaggtata accctgcatc gtgcgatatt gccatttgcc cgcccctaaa
     9181 tagcgagggc agctcgaatc aagcgtaaca ggaatgccac cgggtaaaca aatttgccca
     9241 ccatgggtat gtccacataa catcatatca aagtcagcat gggcagcctg gcggtaaatt
     9301 tcaggagtat gtgacagcaa caacgatacc gcgtcgtgtg gaatggtttg agcagctttt
     9361 tcaagattat ccacacggaa ataatgtgga tcatcgacac ctgctaaaaa aatcgactca
     9421 ccatcttttt ctagtttgac tgcttcattc attaacataa tcacgcccat tgattccagc
     9481 gcgggtagca tacgaataga atcgtgattg ccaagtacac cgtaaatcgg ttgcttgagc
     9541 atcgcaatca accgctgcat gcctgcaata gcgtgatcga tagtgccaaa ggtttgcgcg
     9601 cgataatcgc cagtgagcac acaaatatcg tagggtaaat caatgatcgt ttcgatgagc
     9661 gcagtaagat tacgatcatc catatccaca tgcaaatcgg tcaaatgtaa aatacggaat
     9721 tgatgaaatg cttcgggcaa gtgcgctaaa tagaacgagt tctctctgac ttgtaaatta
     9781 cgtgcattac gttgaccgcg cccatacaag ccaactaatt gaatggcaaa tcgaattagc
     9841 gtatgtgtag cgtaccagtt ttctgggtga aaaaaattca acccttttcc gaaaacttgt
     9901 tgcttgtgat cgctttcgat accgagtcgc tgccgtgcat gaatgcgtcc caatcgttca
     9961 gataattgtt gctcaagggt gtccatgaaa tgaaagttat tcgttgactg atagttagca
    10021 aattatcaga gattcaaacg aatcgctact ggcgcgaaat tgctgataaa tttttataac
    10081 ttaattacta ctattcatag acttacttaa aaacaacact aaatatggca tctattataa
    10141 attcatccat ttcaattgcc cgctacaatt tataccatta acattaaaag ataagtggtt
    10201 aatcttaaga agcgtataag tttagagagt tacaatgatt tgtctgagtt tcaatttctg
    10261 atttatacct cgtaaaagat atgtttcgaa gtgacttgaa gatgagcgac ccaatactga
    10321 accgatcatg agcatgatcc aacaaacaaa atccgaattg ctcgaagaag tacggcaatt
    10381 acgcgctgaa ttagctgccg tcaagtggat gacagaaaag cgagcgcaag agctttatat
    10441 gagcgaagaa cgcttctcgt tagccatgcg aggtgccaat gatgggttat gggattggaa
    10501 tctagaaacg gatgaaatct at
//
LOCUS       DAOQKQ010000091        10376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_3035_length_10376_cov_5.374964, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000091 DAOQKQ010000000
VERSION     DAOQKQ010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 10376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10376
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3035_length_10376_cov_5.374964"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..82)
                     /locus_tag="PKZ49_07300"
     tRNA            complement(7..82)
                     /locus_tag="PKZ49_07300"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47..49),aa:Ala,seq:ggc)
     gene            complement(144..1529)
                     /gene="gltX"
                     /locus_tag="PKZ49_07305"
     CDS             complement(144..1529)
                     /gene="gltX"
                     /locus_tag="PKZ49_07305"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HND36407.1"
                     /translation="MVRTRFAPSPTGYLHIGGARTALFSWAFARKHGGKFVLRIEDTD
                     LERSTQESVQAILDGMAWLNLDYDEGPYYQMQRLTRYQEVAEQLLRTNQAYYCYATKE
                     ELDLLREGQRAAGQKPRYDGRWRDNKSIPPQGIKPVIRLKNPLEGEVVFDDLVKGRIS
                     VANNELDDLVLMRSDGTPTYNFGVVLDDLDMQISHVIRGDDHVNNTPRQINILKALSA
                     SLPQYAHVPMILGADGERLSKRHGAVSVMHYHEEGYLPKALVNYLARLGWSHGDDEIF
                     SSEQLIEWFDLESINRAPAKFNPEKLQWLNQHYLKTEAITDIAHLTIPFLEKDNCEIS
                     NREYLHKVVSLLKDRVSTISELADAAVYFFRVLAPSEELRAQYFNNETKSILVSLIDR
                     FSSISWDKQLIHDEIKNTATAFGVKLPKIAMPLRVAITGEVHTPSIDAVLEILGKSET
                     LNRLNRQITTL"
     gene            1859..3475
                     /gene="nadB"
                     /locus_tag="PKZ49_07310"
     CDS             1859..3475
                     /gene="nadB"
                     /locus_tag="PKZ49_07310"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HND36408.1"
                     /translation="MQVHFDTLIIGSGLAGLTLALNLANTQKVAIVTKRTLLDSASSW
                     AQGGIAAALSTEDSPDKHIQDTLIAGAGLCDTAITKYVVEQGPAAIQWLISQQVDFTK
                     DAQNETGYHLTREGGHSVRRVIHINDATGQAVQLALIRQTQAHKNISIFENHIAVDLI
                     TNKKLLANPLNQLNRCHGAYILDIKSDKIITFSAQNTVLATGGAGKVYLYTTNPDTAT
                     GDGIAMGWRAGCRVANMEFIQFHPTCLYHPHAKSFLISEAVRGEGGLLKLPNGERFMP
                     SHDERAELAPRDIVARAIDFEMKKRGLDCVYLDISHKPISFLKQHFPTIYSRCLDFGI
                     DITKQPIPVVPAAHYMCGGIITDQNGMTDLNNLYAIGETAFTGLHGANRLASNSLLEC
                     LVFGMASAKHILDQPNQESAALPDWDASRVTDADEEVVISHNWDELRRFMWNYVGIVR
                     TNKRLERAQHRVKLLHEEINEYYTNFRVTGDLLELRNLVTTANLIIQCAMQRHESRGL
                     HSSRDYPNTSSELKNTILQGRGAVGNHLSY"
     gene            complement(3495..4829)
                     /locus_tag="PKZ49_07315"
     CDS             complement(3495..4829)
                     /locus_tag="PKZ49_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 domain-containing protein"
                     /protein_id="HND36409.1"
                     /translation="MYLDTDIKSSDFLPNNHPQRIILHNEVHARPSQRIHLPALIIYV
                     AVFNEGVTREQECNHLRLLPNQANLTPEQLQNNFLRLRLQGYALRWERHTEYTRYTVV
                     QQLPNSANLGATDPELLSYLALPKNWLAGIPGLTIAAIKLAMVHGDLTQMEDLLKTAR
                     SWFGGHPIVASLMGRDGHSLAVTDFMLRESGFERMLVIAPPSTSETRAGRISQRLLEI
                     ETYRLVALKALPVAKQLAPELTDAELQLADITARLESKSTSDQELLDTLIALAARVER
                     ITVEHAYRFAATHAYDRVVNQRISELREKAIPGIQTIGEFMQRRLSPAIATVDATAQR
                     MGYLSARVARTSSLLRTRVDILAETQNQQLLEKLTRGQELQLRLQSTVEGLSIAAISY
                     YVISLLLYGFKAIKAAGWPIHPELVTGLLIPVVIWSVWYITHRIHKKFLESH"
     gene            complement(5249..5806)
                     /locus_tag="PKZ49_07320"
                     /pseudo
     CDS             complement(5249..5806)
                     /locus_tag="PKZ49_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558077.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5856..6242)
                     /gene="rplQ"
                     /locus_tag="PKZ49_07325"
     CDS             complement(5856..6242)
                     /gene="rplQ"
                     /locus_tag="PKZ49_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HND36410.1"
                     /translation="MRHKNGLRKLNRTSSHRILMLRNMANSLLKHELIKTTLPKAKEL
                     RRIVEPLITLGKKPSLANKRNAFSRLRDRDMVVKLFSELGERYKSRNGGYLRILKFGY
                     RKGDNAPLAIVEMLDRPIVGVDAKEQ"
     gene            complement(6232..7239)
                     /gene="rpoA"
                     /locus_tag="PKZ49_07330"
     CDS             complement(6232..7239)
                     /gene="rpoA"
                     /locus_tag="PKZ49_07330"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HND36411.1"
                     /translation="MCMSSSTYLMPRIVDVQNISENTAKVTLEPFERGFGFTLGNALR
                     RVLLSSINGCAATEVKIAGVVHEYSTIDGIQEDVIDILINLKGLVFKIHSGSKIELKL
                     KKSTEGAVTAGDIELSHDVEIINRNHVIAHIIKGGSLDMQITVEKGYGYVPATLRKVV
                     DKESKIGSIMLDASFSPIRRVSYAVQNARVEQRTDLDKLILDVETNGSIDPEEAVRYS
                     ARVLIEQLSFFADLVSTPVLKEYPVSPQIDPILLRPVDDLELTVRSANCLKVENIYYI
                     GDLIQRTEAELLRTPNLGRKSLNEIKEVLASRDLSLGMKLENWPPPNLDNQMKESGHA
                     S"
     gene            complement(7261..7887)
                     /gene="rpsD"
                     /locus_tag="PKZ49_07335"
     CDS             complement(7261..7887)
                     /gene="rpsD"
                     /locus_tag="PKZ49_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HND36412.1"
                     /translation="MARNLQPKCRQCRREGEKLFLKGEKCFSDKCAIERRNYAPGQHG
                     QKKSRSSDYGTQLREKQKVRRMYGLLERQFHNLYKLAEKKRGVTGENLLVLLESRLDS
                     VVFGMGFGASRSESRQIVRHNGILVNNKRVNIPSYQVEPGDVIEITDNSKKQLRIKQA
                     LDNTKERKFPSWLDIDVNNLKGTFKSNPDRSDLSSMINESLIVELYSK"
     gene            complement(7898..8287)
                     /gene="rpsK"
                     /locus_tag="PKZ49_07340"
     CDS             complement(7898..8287)
                     /gene="rpsK"
                     /locus_tag="PKZ49_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HND36413.1"
                     /translation="MVKTASRGKKKIKKNVVEGIAHIHASFNNTIVTITDRQGNALSW
                     ATAGGAGFKGSRKSTPFAAQVAAENASKIALEYGVKNLEVRVKGPGPGRDSAVRSLNA
                     AGFKISSISDVTPVPHNGCRAPKRRRI"
     gene            complement(8315..8668)
                     /gene="rpsM"
                     /locus_tag="PKZ49_07345"
     CDS             complement(8315..8668)
                     /gene="rpsM"
                     /locus_tag="PKZ49_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HND36414.1"
                     /translation="MARIAGVNIPNNQHLRIALTAIYGIGIPTAKKICLDLNLDGSVK
                     MKDVADSQIDLIRDYISRMTVEGDLRREITMNIKRLIDIGSYRGMRHKRGLPVRGQRT
                     RTNARTRKGPKKSAN"
     gene            complement(8709..8822)
                     /gene="rpmJ"
                     /locus_tag="PKZ49_07350"
     CDS             complement(8709..8822)
                     /gene="rpmJ"
                     /locus_tag="PKZ49_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HND36415.1"
                     /translation="MKVKASVKKICRNCKIVKREGIVRVICVNLRHKQRQG"
     gene            complement(8858..9076)
                     /gene="infA"
                     /locus_tag="PKZ49_07355"
     CDS             complement(8858..9076)
                     /gene="infA"
                     /locus_tag="PKZ49_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HND36416.1"
                     /translation="MSKEETIQLQGEIIETLPNATFKVKLENGHVVLGYISGKMRMNY
                     IRILPGDKVTVDMTPYDLSKGRITFRSK"
     gene            complement(9093..>10376)
                     /gene="secY"
                     /locus_tag="PKZ49_07360"
     CDS             complement(9093..>10376)
                     /gene="secY"
                     /locus_tag="PKZ49_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HND36417.1"
                     /translation="DLKIRLWFLLGALIVYRIGSHVPVPGIDAIVLKDIFESQDNGLL
                     GMFNMFSGGALSRFSIFALGIMPYISASIIMQLGTVAIPYLEMLKKEGESGRKRITQY
                     TRIGTIFLALIQSFGISIALQSQPGLVITPGTIFVITTVVTLVTGTIFLMWLGEQITE
                     RGIGNGISLIIFAGITAGLPSAIGGTLELVRTGSMHFLVAFGIFVAVISVTALVVFVE
                     RGQRKILVNYAKRQVGKQVLGGQSSHLPLKLNMAGVIPPIFASSIILFPATLAGWFST
                     DTTFSWLSDFSASLSPGQPAYVLLYASMIIFFCFFYTALVFNPKETADNLKKSGAFIP
                     GIRPGEQTTSYIEKIMLRLTLIGSIYVTLVCLLPELLILKWNVPFYFGGTSLLIIVVV
                     TMDFMAQVQSHVMSAQYESLLKKANLKMNNLGLAK"
BASE COUNT         3186 a         2209 c         1722 g         3259 t
ORIGIN      
        1 aaaaactggt ggagataagc gggatcgaac cgctgacctc ttgcatgcca tgcaagcgct
       61 ctcccagctg agctataccc cctagactga aagaataggc gattatactg acgctaaata
      121 tccttgtaaa gcgatagata aatttacaaa gtcgtaattt gccgatttaa tcgattcaat
      181 gtctccgatt ttccaagtat ctcaagtacc gcatcaatcg aaggtgtatg gacttcaccg
      241 gtaattgcaa ctctcaaggg cattgcaatc ttaggcagtt taacaccaaa cgctgtcgct
      301 gtatttttaa tttcgtcatg aatcaattgt ttatcccagc taatcgaaga aaatcgatcg
      361 attagagaaa ccagaattga ttttgtttca ttattgaaat attgcgctct caactcttca
      421 gaaggcgcta atactcgaaa aaaataaaca gcagcatcag caagttcact tatcgtactc
      481 actcgatctt tgagtaaact cactacttta tgcaagtatt cacgattaga aatttcacaa
      541 ttatcttttt ctagaaaagg aatggttaaa tgagcaatat cagtaatagc ttctgttttg
      601 aggtaatgct gattaagcca ctgcaatttt tctggattaa atttagctgg cgcacgattg
      661 attgattcaa gatcaaacca ttcaatcaat tgttcactag aaaaaatttc atcatcacca
      721 tgagaccatc ctaaacgggc cagatagttt accaacgctt taggcaaata accctcttca
      781 tgataatgca tcactgatac cgcgccatga cgtttagaaa gccgctcacc atctgcacct
      841 aaaatcatcg gcacatgtgc atactgaggt aacgatgcac taagcgcctt caaaatgttg
      901 atctgacgcg gtgtattatt gacatgatca tctcctcgaa tcacatggct aatttgcata
      961 tcaagatcat ccaatacaac cccaaaatta taggtcggtg tcccatcact tctcattagc
     1021 actagatcat caagttcgtt attcgcaaca ctaatcctgc ctttgactaa atcatcaaag
     1081 acaacttctc cctctagcgg atttttcaat cgaatcaccg gctttatccc ctgcggtggt
     1141 attgacttat tatcgcgcca acgaccatca tagcggggtt tttgtccagc tgctcgctga
     1201 ccctctctta ataaatcaag ttcttctttg gttgcataac aataatacgc ttgattagtt
     1261 cgcaatagct gttcagcaac ttcttgataa cgagtcaatc gttgcatctg ataataaggg
     1321 ccctcgtcat aatctaaatt taaccaggcc atgccatcta aaatcgcttg tacagattct
     1381 tgcgttgagc gctctagatc tgtgtcttca atacgtaaaa caaatttacc accatgctta
     1441 cgtgcaaatg cccatgaaaa caatgcagta cgcgcaccac caatatggag ataccctgta
     1501 gggcttggag caaatcgagt tcgaaccatc ataagattat ttcagatcaa aaaactagtg
     1561 attttgttag agattttaaa aaataatgtc agggtaaaaa atcaggtaga ttttcagttt
     1621 aactggtaga tattcaccct aattccattc acacgatcaa agaacaggag caagcgatta
     1681 atcgtcagct aattctacta cacccttagc aacctcgctt gtattagaat gctctagcac
     1741 ttagccacgc gataacgcaa aaaatgttat agggtatatc agcaaacgca gttttcctct
     1801 cgtgctagaa taaaaacaac agtagctcta tatttagtta ataaataata ataaacatat
     1861 gcaagttcat tttgatacgc tcatcattgg gagcggcttg gctggactca cgctcgcact
     1921 taatttagca aatactcaga aagttgctat tgtcacaaaa cggactctgc ttgatagtgc
     1981 aagtagctgg gctcaaggtg gtattgccgc tgccttatct actgaagatt ctcccgacaa
     2041 acacatccaa gatacgctta ttgcgggtgc tggactatgc gataccgcaa taacgaaata
     2101 tgtcgtcgaa caaggccctg cagccattca gtggctaatt tcacaacagg tcgatttcac
     2161 caaagatgct caaaatgaaa ctgggtacca tttaacgcgc gaaggggggc acagtgttcg
     2221 tcgcgtcatc catatcaatg atgcaacagg tcaggcagtt caacttgctt taattcggca
     2281 gacccaagca cataaaaata tttcgatctt cgagaatcat atcgcggttg acttgattac
     2341 aaataaaaaa ttattagcca atccattaaa tcaactgaat cgatgccatg gcgcttatat
     2401 cttagatatc aaatcagata aaatcatcac attctcggca caaaacacgg ttcttgcaac
     2461 agggggggcg ggcaaagttt atctttacac cactaacccg gataccgcaa cgggtgatgg
     2521 catcgccatg ggatggcggg caggatgccg cgtagctaac atggaattca tccaatttca
     2581 tccaacgtgt ctttaccacc ctcatgcaaa atcattttta atcagtgaag cggttcgcgg
     2641 tgaaggcggt ttattaaagc tacccaatgg tgaacgcttt atgccttcac atgatgaacg
     2701 tgcagaactt gctcctcgcg atattgtcgc tcgcgccatc gattttgaaa tgaagaagcg
     2761 tggccttgat tgtgtctatc ttgatatctc ccataaacca atctcatttc ttaaacagca
     2821 cttcccaacg atttattcac gatgccttga ttttggtatt gatatcacaa aacaaccgat
     2881 tccggttgta ccagcagcgc attatatgtg tgggggtatc ataacagacc aaaatggaat
     2941 gactgatttg aataacttat atgcaattgg tgaaactgca tttactggtc ttcatggcgc
     3001 caatcgatta gcaagcaact ccttactaga gtgtttagta ttcggaatgg cctcagcaaa
     3061 acatatatta gatcaaccta accaggaatc tgctgccttg ccggattggg atgcaagtcg
     3121 tgtaactgat gcggatgagg aagtcgtaat ttcccataat tgggatgaat tacgtcgatt
     3181 catgtggaat tatgtaggaa ttgttagaac caacaagcgc cttgaacgcg cgcaacatcg
     3241 ggtcaaattg ctgcatgaag aaatcaacga gtactacacg aatttccgtg taaccggtga
     3301 tttattagag ctacgtaatt tagttacaac agcgaatctg atcatccaat gcgccatgca
     3361 acgccatgaa agccgtggac tacattctag tcgtgattat cctaatacaa gctctgaatt
     3421 aaaaaatacg attttacagg gccgtggcgc cgttggaaat catctaagtt attagcttta
     3481 aggatcatag aaggttaatg tgattctaag aattttttat gaattcggtg tgttatatac
     3541 cacacagacc aaatgaccac tggaattagt aatcccgtta ccaattctgg atgaatcggc
     3601 catcctgcag cttttattgc tttgaaccca taaagcaaca aactaattac ataataggaa
     3661 attgcagcaa tcgatagtcc ttctactgta ctttgcaggc gtaactgcaa ttcctgccca
     3721 cgtgttaatt tttccaatag ttgttgattt tgagtctcag ctaaaatatc gactcgtgtc
     3781 cgtaacaaag aactcgtcct tgcaactcga gctgaaagat aacccatgcg ctgcgctgtt
     3841 gcatccactg ttgctatcgc tggagaaaga cgtcgctgca tgaactctcc aatcgtttgg
     3901 ataccaggta tcgctttctc tcttaattca ctgattcttt gatttactac tcgatcataa
     3961 gcgtgagttg cagcaaaacg ataagcatgc tcaacggtaa tacgttccac tctagccgca
     4021 agcgctatta gtgtatctag taattcctga tctgaagtcg acttactctc cagtcgtgca
     4081 gtaatatcag ccagttgtag ctcagcatca gtcaactcgg gagccaattg tttggcaacg
     4141 ggaagcgctt ttagtgcaac tagtcggtac gtctcaattt caagtaagcg ttgtgaaatt
     4201 ctccccgcac gcgtttcaga tgtactcggt ggtgcaatga caagcattct ttcaaagccg
     4261 ctctcacgca gcatgaaatc agttactgca agtgaatggc catctcttcc catcaaggaa
     4321 gccacaatgg gatgtcctcc aaaccaactg cgcgcagtct tcaacagatc ttccatttgc
     4381 gttaaatcac catgcaccat tgcaagttta attgcagcaa tcgtcaatcc tggaattccg
     4441 gccaaccaat ttttaggtaa agccaaataa gataacaatt ctggatcggt tgcacctaaa
     4501 ttggcgctat tcggcaactg ctgcactact gtataacgcg tgtattcagt atgcctctcc
     4561 catctcaagg catatccttg aagccgcaag cgtaaaaaat tattttgcaa ttgctcaggc
     4621 gttaagtttg cctgattcgg caataagcgt aagtgattac attcttgttc tcgtgttaca
     4681 ccttcattaa aaactgcaac ataaatgata agtgcaggaa gatgaatccg ctgacttgga
     4741 cgtgcatgta cttcattatg cagtattatt cgttgcgggt gattatttgg taaaaagtct
     4801 gatgatttga tatcagtgtc taggtacatc gttccaggct tatgaacagc gagctatgtt
     4861 ttttttaaat gtatcaagaa tctgacataa ggtcaaaagt aagtcgccct taatttgcag
     4921 gaaattttat ttcttgataa agaagtggat gtggcaatat acactaagga gtcaaaacta
     4981 agaagttatt ctctgcatga ttctttttat aaataaaaga agtacagaaa atgagcaaga
     5041 aacgcagaaa tccttttgca gaatttagat taagaatcgt gctgccggca atcaaggatg
     5101 acaataggct agtcgaggta tcacggcaat ataatgtagc gactactaca ctaaaccgga
     5161 tacattcctt aaaacaacta aaactctaga ctagcagagg ctaatagcat tcgatgagct
     5221 accatagtac actgacaagt gcatcagatt attttttaca gcgccattca gattttttta
     5281 aatatagaat aaagttagcc gaaacatcat taaactttgt aaagttccaa aatattcgat
     5341 acccgttata atatgaccgt gattatctca ggtaaattca ttatcaccat gcgatactcg
     5401 aaaatgctta gaatagctta catctaccag actattataa ccacaccaac cgtcactata
     5461 aatgacactc gcaatggata tttgcccaat attactgctt gtaaattaat cgcttaaatt
     5521 caggtactat ctcggtgtgc ccatttctct caaagaaccc aaatactggt cgttttaatg
     5581 tgccacgacc acgtttgagt ttgctatgat ataacctcaa tgatgcttcc attaaagtga
     5641 ctcttatcta ctgttagtct acctaacaat ttatccttaa gggcagtctg ggcatgataa
     5701 ttacttgcct gaaagctata tcaacgatca attttgtttc aatttttctt taacaacaga
     5761 gtcctttgct ggcaggtata tcaattaaat tactatttct taatatctgc ctagactaat
     5821 agattttgtc tgctagtcta aagtagactt cagcattatt gctccttagc atcaactcca
     5881 actattggtc tatccaacat ttcaactatc gcaagtggag cattatcacc ttttctatag
     5941 ccgaatttca gtattcttaa ataaccgcca tttctacttt tataacgctc tccaagctca
     6001 gagaataatt taacaaccat atccctatca cgtaagcgac tgaatgcatt tcttttattt
     6061 gcaagtgaag gcttctttcc caaggttatc aatggctcta caattctcct aagctccttt
     6121 gcttttggta gagtagtttt aattaactcg tgttttaata aagagttcgc catatttcta
     6181 agcattaata ttctatgact actagtgcga tttaattttc gtaatccatt cttatgacgc
     6241 atgtcctgat tccttcattt gattatctag attaggaggt ggccaatttt ctaatttcat
     6301 tcctaatgac aaatctcttg atgctaaaac ttctttaatt tcatttaatg attttcttcc
     6361 taaattaggg gttctaagta attctgcttc tgttctttga attaaatcac caatatagta
     6421 aatattctca acctttaaac aatttgcaga tctaactgtt aattccaaat catctacagg
     6481 cctaagcaat attggatcta tttgtggaga aactggatat tctttaagca caggagtgct
     6541 cactaaatca gcaaaaaaag acaattgttc tattaataca cgcgcactat aacgaactgc
     6601 ctcctcagga tcaattgaac catttgtttc aacatccagt atcaatttat caagatctgt
     6661 acgttgctcc acacgagcat tttgtactgc ataacttact cgtctaatag gactaaaaga
     6721 agcatctagc ataatactac caattttact ttctttatct accacttttc gcaaagtagc
     6781 aggaacatat ccatatcctt tttcaaccgt aatttgcata tccaaactac cacctttaat
     6841 gatatgagca atcacgtgat tacgattaat aatctctaca tcatggctta gttctatatc
     6901 gccagctgtt acagcgcctt ctgtagattt tttcaatttc aattcaattt tacttccaga
     6961 gtggattttg aatactaaac ctttaaggtt tatcaagata tcaataacat cttcttgaat
     7021 accatcaatt gtagaatatt catgcacaac acccgcaatt tttacttctg tagcagcgca
     7081 tccgtttatc gatgaaagta atactcgtct aagtgcgttt cctaaagtaa atccaaaacc
     7141 tcgctcaaaa ggttccaaag ttacttttgc agtattttca gagatattct gaacatcaac
     7201 tatcctaggc atcagatatg tactactaga catacacatt cacctttatt ataaaattca
     7261 ttatttcgag tataactcga ctatcaaaga ctcattgatc attgatgata aatctgatcg
     7321 atctggattt gatttaaatg tgcctttaag attattcaca tcaatatcta accaactagg
     7381 aaattttcgc tctttggtat tatctagcgc ttgtttgatt ctcagttgtt ttttagaatt
     7441 atctgtaatt tcaataacat cacctggctc tacttgatat gaaggaatat ttactctttt
     7501 attatttaca agaatgccat tatgccgaac aatttgccga gattcagaac gtgatgcacc
     7561 aaaacccata ccaaatacta cactatctaa tctactctca agtaatacaa gtaaattctc
     7621 cccagtaact cctcttttct tttcagccaa tttataaaga ttatgaaatt ggcgctctaa
     7681 gaggccgtac atacgtcgta ctttctgttt ttctcttagc tgcgttccat aatcagagga
     7741 cctgctcttt ttctggccat gttgtcccgg agcataattt ctgcgttcaa ttgcacattt
     7801 atcagaaaaa catttttcac cttttaaaaa taatttttca ccttcccgac gacattgacg
     7861 acacttaggt tgtagatttc tagccacttt atatccttta tattcttctg cgtttaggcg
     7921 cacggcatcc attgtgagga acaggtgtta catctgaaat actagaaatt ttaaaacccg
     7981 cagcattaag agaacgcact gcagaatctc ttcctggtcc agggccttta actctaacct
     8041 ctaaattttt aacaccatat tcaagagcta ttttactagc attctctgca gctacttgcg
     8101 cagcaaaagg agtgcttttc cgggatcctt taaaaccagc acctcctgca gttgcccaag
     8161 acagggcatt tccttgtcta tctgttatag ttacaatagt attattgaaa gaagcgtgga
     8221 tatgcgctat tccctcaaca acattctttt ttatcttctt tttacctcga ctagcagttt
     8281 taaccatatt tattaacaaa attattgatt aactttaatt agcagatttt ttaggacctt
     8341 tccgtgtcct tgcatttgtt cgagttcgtt gtcctcttac aggaagtcct cttttatgtc
     8401 gcatacctct ataacttcca atatctatta atctcttaat attcatagtt atctcacgtc
     8461 gcagatcacc ctcgacagtc attctagaaa tataatctct aattagatca atttgcgaat
     8521 ctgcaacatc cttcatcttt acactaccat ctaaattcag atctaaacag attttctttg
     8581 cggtaggaat tcctatacca taaattgctg ttagtgcaat tcttagatgt tgattattag
     8641 gaatgttgac acctgcaatt ctagccattt attaatacct aatcttaagt ttacttgaat
     8701 tttaaagttt atccttgtct ttgtttgtgt ctaagattga cacagataac tcggactatc
     8761 ccttcacgtt ttacaatttt acaatttcta caaatttttt taactgatgc tttgactttc
     8821 ataatatata ttttaaatga attaaatgac gtatcactta tttacttcta aaagttattc
     8881 ttccttttga taaatcgtat ggagtcatat caaccgttac tttatcgcca ggtagtattc
     8941 gaatataatt cattctcatt tttccagata tataacctaa tactacatga ccgttttcta
     9001 atttaacctt aaaagttgca tttggtagag tttctatgat ttccccttgt aattgtattg
     9061 tttcttcttt tgacatgaca tcaaaataat ttttactttg caagtcctaa attattcatt
     9121 ttaaggtttg cttttttcaa taaactctca tattgcgcgc tcatcacatg ggactgaacc
     9181 tgtgccataa aatccattgt aacaactacg atgatcaata atgaagttcc tccaaaataa
     9241 aaagggacgt tccactttaa aattaataat tctggcaata aacatacaag agttacatat
     9301 atagaaccaa taagagtcag tctaagcatg attttctcaa tataactcgt tgtttgttcc
     9361 ccaggtctta ttcctggaat aaaggcacca ctttttttaa gattatcagc agtttctttt
     9421 ggattaaaaa ctaatgcagt ataaaagaaa caaaagaaaa taatcatgct tgcatataat
     9481 aatacataag caggctgacc aggagataat gaggcactaa aatcacttaa ccacgaaaaa
     9541 gtagtatctg tagaaaacca tccagctaaa gtagctggaa acaagataat acttgaagca
     9601 aatattggtg gaattacgcc tgccatatta agttttaatg gtaaatgaga gctttgtccc
     9661 cccaatacct gtttacctac ttgtcgtttt gcgtaattca caagaatttt tctctgccct
     9721 ctttcgacaa atacaactaa tgcagtaacg ctaataactg caacaaatat accaaacgca
     9781 acaaggaaat gcatagatcc ggtccgtacc aattctaacg taccgcctat cgcactgggt
     9841 aatcctgcag taattccagc aaagatgatt aacgaaatgc cattaccaat accacgttca
     9901 gtaatttgtt ctcctaacca cataagaaaa attgttcctg ttacaagtgt taccacagtt
     9961 gtaataacaa atatagttcc aggggtgatt actaaaccag gctgcgattg cagggcaatc
    10021 gatataccaa agctttgtat caatgctaga aaaattgtac caatccgcgt gtattgggtg
    10081 attctttttc gcccactttc accttctttt tttaacattt cgagataagg tattgcaact
    10141 gtacctaatt gcataataat cgatgcagaa atatatggca ttatccctaa tgcaaaaatt
    10201 gaaaaccgtg ataacgcacc gcctgagaac atattaaaca ttcccaataa accattatct
    10261 tgactctcaa aaatatcctt taatactatc gcatctattc caggcacggg cacatgagaa
    10321 cctattctat atactattaa tgcgcccaac aaaaaccaca atcttatttt tagatc
//
LOCUS       DAOQKQ010000092        10227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_3092_length_10227_cov_6.505505, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000092 DAOQKQ010000000
VERSION     DAOQKQ010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 10227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10227
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3092_length_10227_cov_6.505505"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..2697
                     /gene="cphA"
                     /locus_tag="PKZ49_07365"
     CDS             121..2697
                     /gene="cphA"
                     /locus_tag="PKZ49_07365"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HND36418.1"
                     /translation="MKLIQSRVLRGPNLWSKHPSIEALLLFDEGACLIEDIPEFESRL
                     RDRFPEIALQYPANSHDVRTIAQVLEFAVLGLQNQIGCPVTFGQTISMPDKRNYRVIV
                     EYSEEAVGQLAFQYAHDLCIAAIENLPFNLNHALQHMRTLYEDIRLGPSTGAIVKAAI
                     ARGIPYRRLTDGSLVQFGWGSKQRRIQGTESDATNAIAESIVQDKQLTKALLQAAGIP
                     VPLGRCVMNADDAWVAACEIGLPVVIKPKDGNHGRGVAVNLMTEQQVRLAFTLATAAE
                     DEVIVEQYVTGSDYRLLVVGEKVIAAARRDPPQVIGNGVHTISQLVEQINRDPLRSEG
                     HSNLLSKIHLDQISLDYLATQGYSAESIPNKGVRVILRNNANLSTGGTATDVTDDVHP
                     EIFATAIAAAKMTGLDICGIDMICDSIRVPLEEQRGAVIEVNAAPGLRMHLQPSFGKG
                     RAVGESIINYMFAPGENGRIPIVAVTGTNGKTTTVRLITNILSIHGMQVGMTCTDGIF
                     VDSDGIDTGDCSGPKSARNVLFHPKVDAAVLETARGGMLREGLGFDHCDVAVVTNIGR
                     GDHLGLAHINTSEEVALVKRIVIENVLPKRGVAILNADDPLVVKMADHSPGSVIFFTQ
                     NSRNPVMLTHRIQQKRVIYLDKKTIIASSIDTEQRFALSEIPLTQNGLIGFQIQNVMA
                     AIGAGWALGIDWATIRAGLANFVSDVQTVPGRFNFFNYRNATLIADYGHNPDAIEALV
                     DAIDNLPAKKRIVVISAAGDRRNEDIRLQTQILGDVFDEIVLYQDQCQRGRSDGEVLG
                     LLREGLEHAKRVSKVREIHGEFKAIDTALTNLQAGELCLILVDQVEQSLNHIRERVTS
                     HR"
     gene            complement(2722..3198)
                     /locus_tag="PKZ49_07370"
     CDS             complement(2722..3198)
                     /locus_tag="PKZ49_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36419.1"
                     /translation="MKKIIISTLICFFVLPLNAANETDNRKALLLNEAQRHHILSEMR
                     SMLLGTQQIIQALAEDDMLAVARYARMLGMEMAHKGEDHLQSVLPDDFMQLGMSVHRS
                     FDKIATDAETLKDSRHTLSQLSMTLQQCVACHSAYQIWTTDIPSDAEPHPTHHLSH"
     gene            complement(3239..5626)
                     /locus_tag="PKZ49_07375"
     CDS             complement(3239..5626)
                     /locus_tag="PKZ49_07375"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HND36420.1"
                     /translation="MTHSIINKNKLFLGLAFFLLGLGGIFWFVLSASLPQYSGSAKLS
                     SLTQAVRVDRDALGTATIQAMNRLDLTQALGYVHAQERFFEMDLMRRSAAGELAELFG
                     KAALPKDRQIRKHRMRTRAKHMFDNLPEDQRALIESYRKGVNAGLATLLIQSFPYLLI
                     RERPVPWQNEDTLLVILAMYFTLQESSLNRELQLSALQTVLPASVYQFLTASGGDWDA
                     PLIGIPLKWPQLPSKEEFNFQAYAHLPTMEQDRLELHDEAPGSNSFAVSGSLTDGAAL
                     IANDMHLTLRVPNIWFRTRLIYPHPNKAGINVAVSGVTLPGVPAIVVGSNGNVAWSFT
                     NSYIDVADWVRVVRHPEHSTRYLSDGDWKSLATFQEILHVRDAEDEFLEVEETEWGPI
                     LGHDHDGTALALKWVAQHRDAADLKLMRLEWVETAHQALEIAQQSGIPAQNFIVGDRE
                     GSIGWTIAGRIPMRSGNYDPNFPSDWPNGETGWLGWLPSAHYPLISNPMNMRLWSANA
                     RMIDGLALVKLGDGGYELGARSKQIRDNLFAKEKFTALDLFEVQLDHRALFLSRWQKL
                     LHEVIDHAPIEPWKQKLEDGLLDWDGLAVPSSVSYRIVRTFRQEVVDSIYRNFIAVVK
                     HQYPDFSPPRLPQIEHAIWRLIENRPPHLLPLGNANWEAFLQKCARKVALLMETQSGD
                     DKPWNWGGQNTAQIQHPLSKVLPNFVAWWLNMPKDLLPGDSFMPRIQAPSFGASERFV
                     VAPGNEEQGYFSMPGGQSGHPLSPYYGSGHQDWVSGKPTPFLPGIAQQTLWLAPH"
     gene            complement(5637..6134)
                     /locus_tag="PKZ49_07380"
     CDS             complement(5637..6134)
                     /locus_tag="PKZ49_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HND36421.1"
                     /translation="MIALKWFLVLILILVIVAILAGQLGMFKGGAPNDLGVVEGRLKL
                     PADTPNSVSSQADLYPNHPQHNYARIEPLPLVGDSKSTLSKIKAIIELMPGSQIITNQ
                     ENYLYVQFTTRVMKFVDDAEFWFDPQSNVVQVRSASRLGKSDLGVNRQRIEWIRKQLE
                     EISLK"
     gene            6185..7045
                     /locus_tag="PKZ49_07385"
     CDS             6185..7045
                     /locus_tag="PKZ49_07385"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HND36422.1"
                     /translation="MPESRNNLSTQLIQDPNELPLIFHSRVNEPISNLSLLRKSLLFA
                     ELSMIAYNDSNEAKRAVNLIGFPVSIFFDYDGAQGFFFENDHDAVIVCRGTEPNEWND
                     IHADANLGTVLAETAGRVHRGFKQEADDLWPMMEKHLIDLGKTLWFTGHSLGAAMATV
                     CAYRCHVSNKCASPTELYTFGSPRVGNKRYVNFVKLKHYRWVNNNDIVTRVPPIWLGY
                     RHIGIEMYLNRNGLLRTLDYATKRLDRWHGFLRGIRKFRIDHFSDHSIHEYVNHILKA
                     VQKDENNAAS"
     gene            7227..8456
                     /locus_tag="PKZ49_07390"
     CDS             7227..8456
                     /locus_tag="PKZ49_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase, exosortase A
                     system-associated"
                     /protein_id="HND36423.1"
                     /translation="MRILHILDHSIPLHSGYTFRTRSILKAQRALGWETFHLTSTKQG
                     LTNSLEEVIDNFHFYRTPVNNRFINTLPIINQIAVMTSLTQRLNEVVDIVKPDILHAH
                     SPVLNAIPALYVGNKKGIPVVYEVRAFWEDAAVDHGTSNAGGLRYRLTHGLETYALKR
                     ANAITTICEGLRNDIVERGIEAHKVTTIPNAVDLAQFSMNHSIDQSLKQRLGLQDKRV
                     IGFIGSFYAYEGLEILLRSLPNLLPIYPNLQLLLVGGGPQENFLKQTAQTLGIQDKVI
                     FTGRVPHEQVEKYYDLIEVLVYPRLSMRLTDLVTPLKPLEAMAQGKLFIASDVGGHRE
                     LIRDGETGLLFKSGDPDALAKKIIYLFNTPDLWKSLIQAGRAYVENERNWQNSVARYK
                     TVYNQLLVEQGKTVESL"
     gene            8443..9543
                     /locus_tag="PKZ49_07395"
     CDS             8443..9543
                     /locus_tag="PKZ49_07395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HND36424.1"
                     /translation="MNHYKMHIGLVGPLPPPSGGMANQTRQLAHLLTDENIQVTLIQV
                     NAPYYPQWIEKFKGIRAFFRLIPYLYKLWQASHSVQIFHVMANSGWSWHLFSAPVIWI
                     AYLRNKPVIINYRGGEAEAFLNKSIFWVRPSLKRVSAIIVPSAFLKHVFNQQGFDVTI
                     VPNIIDLDRFAAQTQEWNVPKIENSPHIILTRNLESIYDNATAIRAFSIVKKNFPNAK
                     LSIAGSGPEKLMLEQLVDDLNLVDSVTFLGRVDNTAINDLYRQADVMINTSLIDNMPI
                     SILEALACKVPVVSTNVGGIPFLVKHDETALLVPPKNPGEIAEAIQHILTNPEKSRKM
                     AEAGYEMVQHYAWKNVRTQLMEIYERTLSHRF"
     gene            9568..>10227
                     /locus_tag="PKZ49_07400"
     CDS             9568..>10227
                     /locus_tag="PKZ49_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HND36425.1"
                     /translation="MSTNPVNKHLYTWFIANICFPIHEFFKRHHTVSIRRELEITQWW
                     NKSQIEQLQLSKLKQLLIHAKANVPYYQKIMRDLAIEPEQFESLAALSKLPLLNKAAI
                     RTNIESLKSLNAQSLVRSNTGGSSGEPLIFYLGKERVSHDVAAKWRATRWWNVDIGDP
                     EIVIWGSPIELGTQDNIRALRDSLFRSKLLPAFEMSDQKLQIFLDEIRSFQPKMLFGY
                     PS"
BASE COUNT         3036 a         2201 c         2125 g         2865 t
ORIGIN      
        1 ggcgttagat atatcgcctg atgtaatgcg ggtgggagtc gaagcgtttg gatcaagtca
       61 gcaggaggcg ccttgccaaa gccagttttc atgaaataac aaaaatacaa gggatatagt
      121 atgaaattaa tacaaagtcg tgtattacgc ggtcctaatt tatggagtaa acatccctca
      181 atcgaagctt tattattatt tgatgaaggc gcctgcttga ttgaggacat tcctgaattt
      241 gaatcacgcc tccgtgaccg ctttcctgaa attgcattac agtatccagc taacagccat
      301 gacgttagaa ccattgcaca agtacttgag tttgcggtac tgggcttaca gaaccagatt
      361 gggtgtccag ttacgtttgg acaaaccatt tcaatgcccg ataagcgcaa ctatcgtgtc
      421 attgttgaat atagtgaaga agcagtaggt caactcgctt ttcaatatgc tcatgatctt
      481 tgtatcgctg caatcgaaaa tcttcctttt aatttaaatc acgctttgca gcacatgcgc
      541 acgctttatg aagatattcg cttgggtccc agtactgggg caattgtcaa agcagcgatc
      601 gccagaggaa ttccctatcg tcgtttaacg gatggtagct tggtgcaatt cggttggggc
      661 agcaaacaaa gacgaattca gggcactgaa agcgatgcta ccaatgcaat tgctgaatca
      721 attgttcagg ataaacaatt aactaaagcg ctgttacaag ccgcaggaat cccagtaccg
      781 ctgggacggt gtgttatgaa tgcagatgat gcttgggtag ctgcgtgtga aataggccta
      841 cccgttgtga ttaaaccgaa ggatggtaat catggtcggg gtgtagccgt caatctgatg
      901 acggaacaac aagttagatt agctttcaca cttgccacag cagctgaaga tgaagtgatt
      961 gtggaacaat atgttacagg atcagattat cgtttattag tggtgggaga gaaagtaatt
     1021 gcggcagcaa gacgtgatcc tcctcaagtg attggtaatg gggttcatac tatcagccaa
     1081 ttagttgaac agatcaatcg tgatccatta cgaagtgaag gtcactccaa tttattgagc
     1141 aagatccatc ttgatcaaat ctcattagac tatctcgcta ctcaaggtta ctcagctgaa
     1201 tcaataccaa ataaaggtgt acgtgtcatt ctgcgaaata atgcaaattt atcaacaggt
     1261 ggcacagcaa cagatgtcac ggacgatgtt catcctgaaa tttttgcgac tgcgatagct
     1321 gctgcaaaaa tgactggttt agatatatgt ggtattgata tgatttgtga ctccattcga
     1381 gttcctttag aagaacagcg aggagcggtc atcgaagtaa atgctgcgcc aggtttgcgc
     1441 atgcacttac aaccctcttt tggaaaaggt cgcgcagtgg gtgaatcgat catcaattat
     1501 atgtttgctc ccggtgaaaa tggacgtatt cccattgtcg cagtcacggg aaccaacggt
     1561 aaaacaacta ccgttaggct catcaccaac atacttagta tccatgggat gcaggttggg
     1621 atgacctgca cggatggtat ttttgttgat agtgatggta ttgataccgg cgattgcagc
     1681 ggacccaaaa gcgcgagaaa tgttttattt catcctaagg tagatgctgc ggtgttagaa
     1741 actgcgcggg gagggatgtt gcgtgaagga cttggttttg atcattgcga tgtagcggtc
     1801 gttacaaata ttggtagagg tgatcatcta ggtttagcgc atattaatac ttcagaagaa
     1861 gtggctcttg taaaacgcat cgttattgaa aatgtattgc ctaaaagggg ggtcgcaata
     1921 ctcaatgcgg atgatccgct cgtggttaaa atggctgatc attccccagg ctcagtcatt
     1981 ttctttactc agaatagtcg taatccagtt atgttgaccc atcgtattca gcagaaacgc
     2041 gtgatttatc tggacaaaaa aactatcatc gcatcgagca ttgatactga gcagcgcttt
     2101 gcattgagcg agattcctct aacccaaaat ggtttgattg gattccaaat tcagaatgtc
     2161 atggctgcga ttggtgcagg atgggcgctg ggcatcgatt gggcaacgat tcgagcgggg
     2221 ctagccaatt ttgtaagtga tgtgcagacg gtcccaggcc gctttaattt cttcaattat
     2281 cggaatgcaa ccttgatcgc agattacgga cataatcctg atgccattga agcattggtt
     2341 gatgcaattg ataatctacc tgcaaagaaa cgaatcgttg tgattagcgc agctggtgat
     2401 cgtcgtaatg aagatatccg gttacaaacc caaattttgg gtgatgtttt cgatgagatc
     2461 gtgctatatc aagatcaatg ccagcgggga cgttccgatg gagaagttct aggactgctg
     2521 cgagaaggat tagagcatgc taagcgtgtt agcaaagtga gggaaatcca cggtgaattt
     2581 aaagcaatcg atacggcatt aaccaatctt caagccggcg aattgtgctt gattctggtt
     2641 gatcaagttg aacaatcctt aaatcatatt cgcgaacggg taacgtcaca tcgctgactg
     2701 agagccaatt tcatcgatta tttaatgact cagatggtga gtcgggtgcg gttcagcatc
     2761 agaaggtata tcggttgtcc agatttgata cgccgagtga catgcaacac attgctgcaa
     2821 agtcatactt aactgagaca atgtatgcct agaatctttt agcgtctctg cgtctgtggc
     2881 gatcttatca aagcttctat gaactgacat accgagttgc ataaaatcat cgggcagaac
     2941 agattgcaaa tgatcttcac ctttatgcgc catttccata cccagcatac gcgcatatct
     3001 cgctactgct aacatatcat cttctgcaag tgcttgtatg atttgctgtg tccctaaaag
     3061 catagaccgc atctcggata aaatatgatg gcgctgcgct tcattcagta atagtgcttt
     3121 tcggttatca gtttcattgg ctgcatttaa cggaagcaca aaaaagcata ttagcgtgct
     3181 gataataatt tttttcataa aaaacaccct ctcgtttttt tgttaatctt tacagcactc
     3241 aatgtggcgc caaccacaat gtttgctgcg ctataccagg aagaaatggg gttggtttcc
     3301 cacttaccca atcctgatga ccgcttccat agtaaggtga aagtggatgg ccactttgcc
     3361 caccaggcat agaaaaataa ccttgctctt cattaccagg cgcaacaaca aatcgttcag
     3421 aagccccgaa actcggtgct tggatacgag gcataaaact atcacctggc agtaaatctt
     3481 taggcatatt taaccaccaa gcaacaaaat ttggtaaaac tttacttaac ggatgttgta
     3541 tctgagcagt attctgcccc ccccaattcc aaggtttatc atcgccggat tgcgtttcca
     3601 tcagtaaggc cacttttctt gcacattttt gtaaaaacgc ttcccagttt gcattgccga
     3661 gaggaagcaa atgaggtggg cgattctcta tcaaccgcca tattgcgtgt tcaatttgcg
     3721 gtagtcgcgg aggcgaaaaa tctggatact gatgctttac gaccgcaata aaattacgat
     3781 aaatgctatc aactacttcc tgcctgaaag tacgaacgat tcggtaggaa actgagcttg
     3841 gtactgcaag accatcccaa tccagcaatc catcttctaa tttctgcttc catggttcaa
     3901 tcggtgcatg atctatcact tcatgaagaa gcttctgcca gcgagacaaa aataatgcgc
     3961 gatgatctaa ttgcacctcg aacaaatcta gtgcagtaaa tttttctttg gcaaacaaat
     4021 tgtcacgaat ctgttttgaa cgcgcaccca attcgtatcc accatctcca agttttacta
     4081 atgcaagacc atcgatcatt ctggcattag cactccataa gcgcatattc attggattac
     4141 taatcaaagg gtaatgcgcc gaaggtaacc atcctaacca acctgtttca ccatttggcc
     4201 aatctgaagg aaaattgggg tcataattac cagatctcat cggtatacgc ccagcaatgg
     4261 tccatccaat gcttccttcg cgatcaccca caataaagtt ttgggctggt attccgctct
     4321 gttgcgcaat ctctagtgcc tgatgagcag tttctaccca ctccaagcgc atcaatttaa
     4381 gatcagcagc atcacgatgc tgagcaaccc acttcagcgc aagtgctgta ccgtcatgat
     4441 catgacctag aatgggtccc cactccgtct cttcaacttc aagaaactca tcttcagcat
     4501 cgcggacatg cagaatttct tgaaaggttg ccaatgattt ccaatctccg tcggaaagat
     4561 atcgtgttga atgctcggga tgacgaacaa cacggaccca atccgctaca tcgatatagc
     4621 tattggtgaa actccaggca acattaccat ttgacccaac tacgattgct ggcacaccgg
     4681 gtagcgtaac accactaacc gctacattga ttcctgcttt attgggatgg ggataaatta
     4741 agcgcgtcct aaaccatata ttaggtactc gtagggtaag atgcatatca ttagcaatta
     4801 gcgccgcacc atcggttaaa ctacccgata cagcaaaact attactaccc ggtgcttcat
     4861 catggagttc taaccgatcc tgttccattg ttggcaaatg tgcatatgcc tgaaaattaa
     4921 attcctcctt gcttggtaat tgtggccatt ttaggggtat cccgatgaga ggtgcatccc
     4981 aatccccgcc agatgcggtt aaaaactgat agactgaggc aggtaaaacc gtttgtaaag
     5041 ctgatagctg tagctctcgg ttaagacttg attcttgcag cgtaaaatac atcgccaata
     5101 taactagcaa agtatcttca ttctgccaag gaactggacg ttctcgtatc aataaatagg
     5161 gaaaagactg aatgagtaat gtagctagtc ctgcattaac tcctttacgg tatgactcaa
     5221 ttagagcacg ttgatcctct ggtaagttat caaacatatg cttggctcga gttctcatac
     5281 ggtgttttcg tatttgtcta tcctttggta aagctgcttt accaaataat tccgctaact
     5341 caccggccgc acttcgacgc atcaaatcca tttcaaagaa acgctcttgt gcatgcacat
     5401 aaccaagtgc ctgcgttaaa tcaagtcgat tcatcgcttg aatcgttgcc gtacccaaag
     5461 cgtcacgatc aactctaact gcctgagtca agcttgaaag cttagcagaa cctgagtatt
     5521 gcggtaagct agcgctcaac acaaaccaaa agataccccc taatccaagc agaaaaaaag
     5581 ccaagccgag aaaaagcttg tttttattta taatagaatg agtcatcagt gatctctcat
     5641 tttaatgaga tttcctccaa ctgtttacgg atccactcaa tccgttgtcg attaacacca
     5701 agatcactct tgcctaatcg agaggcagat cgtacttgca cgacatttga ctgaggatca
     5761 aaccaaaact ctgcatcatc gacaaacttc atcacgcgcg ttgtaaattg cacataaaga
     5821 tagttttctt gattggtaat tatttgagag ccaggcatca gctcgataat cgctttaatt
     5881 ttactcaagg tagatttgct atcgcctacc aatggtaatg gctcaattcg ggcatagtta
     5941 tgctgcgggt gattggggta gagatctgct tggcttgata cgctatttgg cgtatccgca
     6001 ggcaatttca gcctaccttc gacaacaccc aggtcgttgg gtgcgccccc cttaaacatc
     6061 ccgagctggc ctgctagaat tgctacaata acaagaatca gtatcaaaac caagaaccac
     6121 tttaacgcta tcatgttagg aaaatcctat taatttaaaa ttaaaaatac aaccaagaaa
     6181 cgttatgcca gaaagtagaa ataatctatc aacacaattg atacaagatc ctaacgaatt
     6241 acctttaatc tttcattccc gcgtcaatga acccatctca aatctatcat tacttcgcaa
     6301 atcattgtta tttgctgaat tatcgatgat cgcttataac gattctaatg aagcaaaacg
     6361 tgctgtcaat ttgattggtt tcccagtgag tatttttttt gattatgatg gcgctcaagg
     6421 ttttttcttc gaaaatgacc acgatgccgt gatcgtttgc cgaggaacag agcccaatga
     6481 gtggaacgat atccatgcag atgctaactt aggaaccgta ttagcagaaa cagcgggacg
     6541 cgttcaccga ggttttaaac aagaagcaga tgatctctgg cccatgatgg aaaaacatct
     6601 aatcgattta ggcaaaacgt tgtggtttac tggtcactca ttaggtgcag ctatggcaac
     6661 cgtgtgcgcc tatcgatgcc atgtttcaaa caaatgtgct agcccaaccg aactctatac
     6721 atttggtagc cctcgcgtcg ggaataaacg gtatgtcaac tttgtcaagc taaagcatta
     6781 tcgttgggtc aataataatg atattgtgac aagagtcccc ccgatatggc tgggttatcg
     6841 tcatatcggc attgaaatgt acctaaaccg taacggtctt ttacgaacac tcgattacgc
     6901 aaccaaacga ttagatcgct ggcacggatt cctacgcggc attcgcaaat tccggataga
     6961 ccacttttct gaccatagca tccatgagta tgttaaccat attttaaaag ccgtacaaaa
     7021 agatgaaaac aacgcagcga gttaacaaat ctcgccatta ccaatcactg actaatcgtt
     7081 aacattgcaa attatcgatc agcccaaatc aatattgcat tagttatata aaaaactact
     7141 gctattttca tctcagttaa gaaaaataaa cagttatgat atgaagtaat ttttattcgt
     7201 ttggcttatt tagctatcta aacataatgc gtatcctgca tatacttgat cattcgattc
     7261 cattacacag cggttataca ttccgaacac gctctattct gaaagcgcaa cgcgcgttag
     7321 gatgggaaac gtttcattta accagcacta agcaaggttt gacaaactcg cttgaagaag
     7381 taatcgataa tttccacttt tatcgaacac ccgttaataa tcgttttatc aacacattgc
     7441 ctatcattaa tcagatagca gtaatgacaa gccttactca acgattaaat gaagtagtag
     7501 atatcgtcaa acccgatata ttgcatgctc actccccggt attaaatgcg ataccagcct
     7561 tatatgttgg caataagaaa ggtattccag ttgtttatga ggtcagagca ttctgggaag
     7621 atgcagcagt tgatcatggg acgagtaacg cagggggcct acgttatcgt ttgacacatg
     7681 gcttagaaac ctatgcatta aagcgtgcta acgccattac cacgatatgt gaaggattgc
     7741 gtaacgatat tgtagaacga ggaattgaag cgcataaagt tacaacaatt cccaatgcgg
     7801 tcgatctcgc acagttttca atgaatcatt cgattgatca atcactgaag cagcgtttgg
     7861 gtcttcagga taagcgcgtc atcgggttta tcggttcctt ttatgcgtac gaaggtttgg
     7921 agatcttatt gcgatcgtta ccaaacctat tacctatata tcccaatctc caactattac
     7981 tagtgggcgg cggacctcaa gaaaatttct taaaacaaac ggctcaaaca cttggaatac
     8041 aagacaaagt tatcttcaca ggccgagtac cccatgagca agtagaaaaa tattatgatc
     8101 taatcgaagt attagtttat ccgcgattat cgatgcgatt aaccgactta gttactccgc
     8161 tgaaaccact tgaagcaatg gcgcaaggta aattatttat tgcttccgac gtaggaggtc
     8221 accgcgaatt aattcgcgat ggcgaaacag gtcttttatt taaatccggc gatccagacg
     8281 cgcttgcaaa aaaaatcatc tatttattta atacacctga tctttggaaa agcttgattc
     8341 aagctggacg tgcttacgta gagaatgaaa gaaattggca aaatagtgtt gcacgttata
     8401 agaccgttta taaccagctc ctcgtcgaac aaggtaaaac cgttgaatca ttataaaatg
     8461 catatcggac tggttggtcc attaccacca ccatccggag gaatggcaaa tcaaacgagg
     8521 cagttagctc atttgctaac ggatgaaaat attcaagtta ccttaattca agtcaatgcc
     8581 ccctattacc cccagtggat tgaaaaattc aaagggatcc gcgctttctt tcgactaatt
     8641 ccttacttgt acaagctatg gcaagcctct cattctgttc aaatttttca tgtgatggcg
     8701 aattcaggtt ggtcatggca tctgttttca gcacctgtaa tttggatcgc atacctgaga
     8761 aataagcctg tcatcatcaa ttaccggggt ggagaagctg aagcctttct taataaatct
     8821 attttttggg tcaggcccag cttaaaaaga gtaagcgcca tcatcgttcc ttccgcattt
     8881 cttaagcatg ttttcaatca gcaaggattt gatgtaacga tcgtacccaa tatcatcgat
     8941 cttgatcgct ttgctgcaca gactcaagaa tggaacgtac ctaaaattga gaatagtccc
     9001 catatcattt taacgcgcaa ccttgaatca atttatgata atgcgacagc aattcgcgca
     9061 ttttctatcg ttaaaaagaa ttttccgaat gcgaagctat cgatagcagg ctcaggccct
     9121 gagaaactga tgctagagca gcttgtagat gaccttaatt tagtagactc cgtaactttc
     9181 ctcggtcgag tggataatac tgctatcaat gatttgtatc gtcaagcgga tgttatgatc
     9241 aatacgagtt taattgataa catgccaatc tcgatattgg aggcactcgc ttgtaaagta
     9301 ccagtagtca gcaccaatgt tggcggcatt ccattcttag tcaaacatga tgaaactgca
     9361 ttactggttc caccgaaaaa tcccggagaa atagcagaag ccatccagca cattctcact
     9421 aacccagaaa aatccagaaa aatggctgag gctggatatg aaatggtaca acactatgcg
     9481 tggaagaatg tgcgaactca attaatggaa atatatgagc gtactctctc tcatcggttc
     9541 taatttaata acgaaaccgt aaattatatg agcaccaatc ctgtcaataa gcatttatat
     9601 acttggttta ttgcaaatat ttgttttccg attcatgaat ttttcaaacg ccatcataca
     9661 gtttcgatta ggagagaact tgaaatcacg caatggtgga ataaaagcca aatagaacag
     9721 cttcagctct caaaactaaa acaactgctc atccatgcaa aagcaaatgt tccttactat
     9781 caaaaaataa tgcgtgacct agcgattgaa cctgagcaat ttgaatcgct tgcagcgtta
     9841 tctaagcttc cacttttaaa taaagcagcg attaggacca atatcgaatc attaaaatcc
     9901 cttaatgctc aatcacttgt tcgatcaaac acaggcggat ctagcggaga acccttaatt
     9961 ttctatttag gtaaagaacg ggtaagccat gatgttgcag caaaatggcg agctacccga
    10021 tggtggaatg ttgatattgg cgatcctgag atcgtgattt ggggatctcc tatcgaatta
    10081 ggaactcagg ataatatccg cgcgttacga gactcattat ttcggagcaa attgctgcct
    10141 gcttttgaaa tgtcagatca gaaattacaa attttccttg atgaaattcg ttcttttcag
    10201 cctaaaatgc tctttggcta cccttca
//
LOCUS       DAOQKQ010000093        10209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_3102_length_10209_cov_5.858578, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000093 DAOQKQ010000000
VERSION     DAOQKQ010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 10209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10209
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3102_length_10209_cov_5.858578"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /locus_tag="PKZ49_07405"
     CDS             <1..1307
                     /locus_tag="PKZ49_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36426.1"
                     /translation="EGLATQVRQVAATIDASEELANVIIRVSTNENSVDGAQSITMGK
                     GDDTVIFDMLNDSRAGLTISDTVAGGDGNDTLVIDGHDTRVSLGASEWTNVTGFETIR
                     LVGNNAAPVSTLIGQNSYNLTLTNDLIDANGGDMIHIINDNDVNNDEEDEVDTATTGT
                     EFGVTIDARTLNAQNHFSYNGEEGASRTTDKFILGDANVNGANVIDGGAVDNDGDTTF
                     AANTDILEVRNTATVTTGDLANVRNVGIIAGSNDQATEQTLVLQLNDTVIDAMVDSYH
                     ASSTTEVETLSVRLNDAADVSAVAGMGIDLDTTGTTAKSAITVDLDTVVGGTDVFKIG
                     YGLLTVNNFTTGTDTIQLSVSKFGLTLDADDIGLVANTDTDADGTNIEIGVAAATAAD
                     TRIVIDTATGDIFYDADGNGAQSQVLIAQINGIAAGDVFIVA"
     gene            1428..2966
                     /locus_tag="PKZ49_07410"
     CDS             1428..2966
                     /locus_tag="PKZ49_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HND36427.1"
                     /translation="METWTGGYVADVNYIYGYYPELNPMRAQLALCGAGIVVPKIEVA
                     CELGFGQGLSINLHAAASDVNWYGTDFNPAQAAFAQQLAQQAKSQAHLFDQSFEAFCQ
                     RLDLPDFDYIGLHGIWSWISNKNRAVLVDFIQRKLKVGGVLYVSYNTYPGWAATVPLR
                     ELMLQHTEFMGSEQQGVIGKIDAAIEFAETLFAVNPRFASDNPQVGVRLKNQNRNYLA
                     HEYFNRDWQPMSFAQISQWLAEAKVQYAGSAHYQDYIDTINLTKAQQELLARLPSGRF
                     YQTVRDFCVNQQFRRDYWIKGARKLSPLEKREQLYEIAVVLTQLRSGFEPKIVGSLSE
                     AKIQPTIFNPLLDCLADNKPKQLGQLVQALKNQKITAAQVLQAALLLSGSGALQCAQG
                     EAAIQSARKKTDALNQYLMQKARSNAEINYLASPVTGGGISVPRFHQLFLLGYQQRQQ
                     PKSISAGELAQFAWRILSSQGQRLTKAGKPIETAEANLAELKSIAEKFLGNDLSVYQA
                     LQII"
     gene            complement(2963..3808)
                     /locus_tag="PKZ49_07415"
     CDS             complement(2963..3808)
                     /locus_tag="PKZ49_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HND36428.1"
                     /translation="MATTILSPNNPMTITLICIGLGFGTGIILALTGAGGTIIAVPLL
                     VLSLNLSVVEAVPIGLLAVSLAALIGASIALAQGNVRYRAAGYLAIMGSIVSPLGIIA
                     AQHLPNMVLTVLFAILLTYIAWRHFKISSAPRTSLSERFSVPCLLNDGSNRLVWNAPC
                     FRALTFSGLIAGFLSGLLGVGGGFIINPALKKISNLSMQTILGTTLAVVALISMVGVI
                     SAMLISTLQWSIAIPFVSSTIVGMLFGRYLARYVTESRSQQLFASLAACVAIVMLYKA
                     ISSVL"
     gene            complement(3783..4136)
                     /locus_tag="PKZ49_07420"
     CDS             complement(3783..4136)
                     /locus_tag="PKZ49_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HND36429.1"
                     /translation="MFKESLTETHPTALSAMLSNRAAAGQACALLKVLANEDRLLILC
                     QLIEGEKNVGELEQALGIRQPTLSQQLTVLRDENLVETERKGKFIYYRLTSQEALQIM
                     KMLYQLFCGHHHTES"
     gene            complement(4186..4620)
                     /locus_tag="PKZ49_07425"
     CDS             complement(4186..4620)
                     /locus_tag="PKZ49_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HND36430.1"
                     /translation="MKTLTHLIALLAGLIFGFGLIVSDMVNPDKVQDFLDLSGSWDPS
                     LAFVMGGAIAVGFLAFTFAKRRNQTFMGTPIHLPTTHLIDKRLVLGSLAFGIGWGMAG
                     ICPGPGLVLVGSGSVQGMVFVAAMLIGMGIFELLERRKQKKP"
     gene            complement(4640..5071)
                     /locus_tag="PKZ49_07430"
     CDS             complement(4640..5071)
                     /locus_tag="PKZ49_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HND36431.1"
                     /translation="MNIDWTSFTPVSALIGGGIIGIATAVLLLFAGRIAGISGIVGGL
                     FKQPSADTGWRIAFLAGLLLAPICWQQFSNLPPIQINANDATLALAGFIVGIGTRYGS
                     GCTSGHGVCGISRLSPRSIVATMLFIATGVMTVYFIRHLIS"
     gene            5167..6027
                     /locus_tag="PKZ49_07435"
     CDS             5167..6027
                     /locus_tag="PKZ49_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011113024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HND36432.1"
                     /translation="MRAQIRAFFDPATWTMSYVVYDEQGGFCAIIDPVLDYDPKSGRT
                     KTVSADKLIDFVRENRLTVEWLLETHAHADHLSAAAYLQDKVGGKTAIGESIALVQGS
                     FKKLFNLGPEFVADGLHFDHRITDGEVLRVGKITLKAISVPGHTPADMAYQLDDTVFV
                     GDTLFMPDVGTARADFPGGDAHQLYHSIQKILSLPPQTRLFMCHDYPPSNRSPQWEST
                     VLEEKMHNIHIKDGVTEDAFVAMRTKRDATLEMPVLMLPSVQVNIRAGRFPDPESNGV
                     SYLKIPINAI"
     gene            6058..7701
                     /locus_tag="PKZ49_07440"
     CDS             6058..7701
                     /locus_tag="PKZ49_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HND36433.1"
                     /translation="MKRFDHARRQFLQAATLGSLVSLRPDLLLAGESQKSTTITSTFK
                     PDVEIELFARNDKVSMMSGAATDVYRYHGQLLQGPSTTLSTMPSYLGPTLNLVQGQKV
                     RIHFHNQLAEPCITHWHGMHVPQRMDGHPMYEVAEGGTYIYEFEVKNPAGTNWYHAHT
                     HELTAKQVYRGLAGLIVISDEQEQQLQLPSGEYDLPIVIQDRRFTESNQLYYRQHGMH
                     ERMVGFLGDTILVNGQINPTMPVKTRAYRLRVFNGSNSRIYKLAWEDGTPLIAIGTDG
                     ALLEKPESYPYIMLAPAERVELWVDFSGREVGSELVLQSLAFHGAAPSMGMRGRMGGG
                     MEGEAMGMMSGLAQGEQFPIAKFRITEKMSESPQLPQTLIKMNRLTARDASNPDRTIP
                     IAISMAHMSWKLNGRSFEMGNVMDIEKIPVNTIQKIRIFHESHQMHGGRGGMGGGMRG
                     GRGGGMGMTMSMAHPIHLHGQQFQILSRQLTDQQATGGYDTVKQGFINSGWKDTVLVM
                     PGEEVTIIKPFEDYTGLYLYHCHNLEHEDLGMMRNFLVS"
     gene            7847..8782
                     /locus_tag="PKZ49_07445"
     CDS             7847..8782
                     /locus_tag="PKZ49_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HND36434.1"
                     /translation="MNVFFKSQFNCFGLCLVSLLLWTSISFANSEADFFKATLTGDGA
                     TVEKLLAEGLDINLKNPDGFTALAVASQKGHDAIVKLLLDKNAEVDAANIQGGTALLL
                     AVKNGHAAIVDTLLAKGADPNLQAKNGLTPLIIAAAKDYADIVKSLVAKGADPDLSDT
                     SQTTALHIAAQNGHVDIVNTLLTNGAKPDLQDVNGASALILAALVGAQEVVQLLLEKG
                     AKADLKATNDFTALILAAQNGQTAVVETLLARGASINQQNQDGITALMWAALHDHIDT
                     VKLLIEKGADTKLKSKSDKTALDMAKDQTIIELLK"
     gene            9040..>10209
                     /locus_tag="PKZ49_07450"
     CDS             9040..>10209
                     /locus_tag="PKZ49_07450"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HND36435.1"
                     /translation="MSFENLNLNPAIIKAIHELGYTAPTPIQQQAIPELILGHDVMAS
                     AQTGTGKTAAFMLPALNRLATPSKIRSRGPRVLVLTPTRELALQVSDATSKFGKYLPH
                     TKVVSILGGMSYPLQNKLLSQVVDILVATPGRLIDHIQRGRIDFSRLEMLVLDEADRM
                     LDMGFIDDVEAIASRTPDTRQTLLFSATLDSSIDKIALRLLKSPKRIQVAAQHAKLDN
                     IEQKLHYVDDLSHKNRLLDHLLRDESLKQAIVFTATKRDADALADDLAVSGHEVAALH
                     GDMNQRERTRTLTRLRAGRLRILVATDVAARGIDVTNITHVINFDLPKFAEDYVHRIG
                     RTGRAGASGVAVSFATGRDSISLRKIERFTGQTIAPFVVPGMESKYKPKTGGGRSH"
BASE COUNT         2929 a         2080 c         2465 g         2735 t
ORIGIN      
        1 tagaaggttt agcaactcaa gttcgtcaag tagctgcgac cattgatgct tctgaagaac
       61 tggctaatgt gatcattcgt gtcagcacca atgaaaacag tgttgacggc gcacaaagca
      121 ttaccatggg caaaggcgat gataccgtaa tctttgacat gttgaacgat tcacgtgccg
      181 gactgacgat ttctgacacc gttgcagggg gtgatggtaa cgatacctta gtgatcgatg
      241 gccatgacac tcgtgtatca ctcggtgcat cagaatggac caatgtgacc ggttttgaaa
      301 ccattcgttt ggtgggtaac aatgctgcgc cagttagcac cttgattggc caaaacagct
      361 ataacctcac gttgaccaat gatctaatcg atgccaacgg tggtgatatg attcatatca
      421 tcaatgataa cgatgttaac aatgatgaag aagatgaagt ggataccgct acaacgggta
      481 ccgaattcgg tgtcactatc gatgcacgta cactgaatgc acaaaaccac ttctcctata
      541 acggtgaaga aggtgcgagc agaaccacag ataaatttat cttgggtgat gcgaatgtta
      601 atggtgccaa tgtaatcgat ggtggtgcag tagataatga tggtgacact acctttgctg
      661 ctaatactga cattctcgaa gtacgtaaca ccgcaactgt aacaacaggc gatttggcaa
      721 atgtcagaaa cgttggaatc attgctggct caaatgacca agcgactgaa caaacactag
      781 tactgcaatt gaacgatacg gttatcgacg ctatggttga tagttatcat gcttcatcaa
      841 ccactgaagt ggaaacttta tctgttcgtt tgaacgatgc agctgatgta tctgctgttg
      901 ctggaatggg catcgatctg gatacaaccg gaacaacggc taagtctgct attactgttg
      961 atcttgatac agttgttggt ggaaccgatg tattcaagat tggttatggt ctgctaaccg
     1021 taaacaactt tacaaccggt accgatacca ttcaattgtc agtcagcaaa tttggattga
     1081 cattggatgc ggatgatatc ggtttagttg ctaatactga tactgatgct gacggtacca
     1141 atatcgagat tggcgttgca gcagcaacag cagcagatac acgtatcgtg attgataccg
     1201 caactggtga tatcttctac gatgcagacg gtaatggtgc acaatctcaa gtgctgattg
     1261 ctcaaatcaa tggtattgct gcaggcgatg tctttatcgt agcttaataa ccgtagtaac
     1321 cgaagttaag tgtgcaacag taatgttgct cactaagtag aaattaaaaa aaccgcccgt
     1381 atttagggcg gttttttttt gatcggattt taaatgaatg gcttgatatg gaaacttgga
     1441 ctggcggtta tgtagcggat gttaattata tttatggcta ttaccctgag ctaaatccaa
     1501 tgcgagcaca gctcgctttg tgcggcgcag gcatagttgt gcctaaaata gaagtggctt
     1561 gtgagctcgg ttttgggcaa ggccttagca tcaatctgca tgcggctgca tctgacgtta
     1621 actggtacgg cacagatttt aacccagctc aagcagcgtt tgcacaacaa ttggcgcaac
     1681 aagccaagtc acaagcacat ttatttgatc aatcatttga agcattttgt cagcggttag
     1741 atttacctga ttttgactat attggcttgc atggtatttg gagctggatt tcaaacaaga
     1801 atcgtgccgt tctcgttgat tttattcaac gtaaactcaa ggttggcggc gttctatatg
     1861 taagctacaa cacctatcct ggatgggctg ctaccgtacc tttgcgagag ctgatgttgc
     1921 aacatactga attcatggga tcagagcagc aaggtgttat tgggaaaatc gatgctgcca
     1981 ttgaatttgc tgaaacgcta tttgctgtaa atccacgatt tgcaagcgat aatccacagg
     2041 tgggcgtccg cctgaaaaac caaaaccgta actatctggc acacgaatat ttcaaccggg
     2101 actggcagcc gatgtctttt gcacaaattt cgcagtggct tgctgaggca aaagtgcaat
     2161 atgctggttc tgcccattat caagattata ttgacacgat caatttgacg aaagcacagc
     2221 aggagttgct tgctcgcttg ccgagtgggc gcttttatca aaccgtacgt gatttctgcg
     2281 tcaatcaaca atttcgcaga gattactgga ttaagggagc tcgaaaatta agtcctttgg
     2341 aaaagagaga gcagctctat gagattgcag ttgttttaac gcaactgcgt tcagggtttg
     2401 aacccaaaat agtgggaagc ttgagcgagg cgaagattca acctactatt tttaatcctc
     2461 tgctggattg tttggctgat aataagccca agcaacttgg acaattggtt caggcgctta
     2521 aaaaccaaaa gattacagca gcgcaggttt tgcaagccgc attgctgttg agtggtagtg
     2581 gggctttaca gtgtgcgcag ggcgaggcag caattcagag tgcccgaaaa aaaactgatg
     2641 ctttgaatca gtatttgatg caaaaagcac gtagtaatgc tgaaattaat tacctggcga
     2701 gtcctgtcac gggcgggggt atttcagtgc cgcgctttca ccaacttttt ttactgggtt
     2761 accaacaacg ccagcaacct aagtcgattt ctgcagggga attagcacaa tttgcctggc
     2821 ggatattgtc ttcgcagggt caacgactta ccaaagcagg taagccaatc gaaacagcgg
     2881 aagcgaattt agctgaatta aaatctatcg ctgaaaaatt tctgggtaat gatttatcgg
     2941 tttatcaggc tttgcaaatt atttagagca cactggagat tgctttatac agcattacga
     3001 ttgccacgca agcagcgagt gaagcaaata gttgttgaga ccgtgattca gtgacatatc
     3061 gcgcgagata tcgaccgaat aacattccta ctatggtact gctaacgaat gggattgcga
     3121 tcgaccattg tagcgtgctg attagcatcg ctgaaatcac accgaccatg gaaatgagtg
     3181 caacgacggc taacgttgtg ccgagtatcg tttgcatcga taagttagaa atttttttga
     3241 gcgcagggtt gatgatgaat cctcccccta cccctaataa gcccgacaaa aaacctgcga
     3301 tcaatccaga aaatgtgagc gcacgaaagc aaggcgcatt ccaaactaag cgattcgagc
     3361 catcattgag taggcaaggt acggaaaatc gctcagagag cgatgttcgt ggcgctgaac
     3421 ttattttaaa atgacgccat gcaatgtaag tgagtagtat tgcaaacaaa acggtgagca
     3481 ccatattggg taaatgctgt gcagctatga tgccgagcgg cgatacgata gaacccataa
     3541 tggcaaggta acctgctgct cgataacgaa catttccttg tgcaagcgcg atcgatgcac
     3601 cgatcaatgc cgctaaacta accgccaata aaccaattgg aactgcttct acaacggata
     3661 gattgagact taatactaaa agcggtactg cgataatggt gccaccagca ccggtaagcg
     3721 caagaatgat accggttcca aagcctaatc caatgcaaat gagtgtaatg gtcattgggt
     3781 tgttaggact cagtatggtg gtggccacaa aataactgat agagcatttt catgatttgt
     3841 aatgcttcct ggcttgtcaa tcggtaataa ataaatttac ctttacgttc cgtttcgact
     3901 agattttcat cgcgtaatac agtcagttgc tgtgacaaag tcggttgtcg gataccaagt
     3961 gcttgctcta attcaccaac gtttttttct ccctcgatta gctggcaaag aatcagtaag
     4021 cgatcttcat tggcgagcac ttttaataat gcacaggcct ggcccgcagc tgctcgattg
     4081 gatagcattg ctgatagtgc ggttgggtga gtttcggtga gtgattcctt aaacatagtt
     4141 tggtgaaaga gggttgaata taatggtggt ggttgtttct taattttaag gtttcttttg
     4201 tttacggcgc tctagcaatt caaatatccc cataccgatt aacatggcag caacaaatac
     4261 cataccttgc acgcttcctg agccgacgag aaccaacccg ggacctgggc aaatacctgc
     4321 cataccccag ccgataccga aagcaagact gccaagtact aatcgtttat cgatgaggtg
     4381 tgtggttggc aaatgaatcg gtgtacccat gaaggtttgg ttgcgtcgtt tggcaaaagt
     4441 aaaagccaga aatcctaccg caatggcacc ccccatcacg aaagctaatg aaggatccca
     4501 tgagccactc agatccagga aatcctgaac tttatcggga ttaaccatat ccgatacaat
     4561 caaaccaaat ccaaagatta ggccagccag tagcgcgatc aaatgagtaa gcgttttcat
     4621 cgtgttcagt tgagttgaat taactgatga gatgacggat gaaataaacc gtcatgacac
     4681 ccgttgcaat aaaaagcatg gttgcgacga tcgatcgagg tgaaagccgg gagattccac
     4741 aaaccccatg accactcgtg caaccggaac cataacgtgt tccgataccg acgatgaatc
     4801 ctgcaagtgc gagtgttgca tcatttgcat tgatttgaat cggaggcaag tttgagaatt
     4861 gttgccaaca gatgggggca aggagaagtc ctgctaaaaa ggcaatgcgc cagcctgtat
     4921 cggcgctggg ttgtttgaat aatccgccaa caatgcctga gattccagcg attcgacctg
     4981 caaacaggag taacactgca gttgcgatac cgatgatgcc gccaccgatt aaagcagata
     5041 caggtgtaaa gctcgtccaa tcaatattca tcgaaatccc caaaatttat ctttacatat
     5101 aatcttttag tatattatat taatatatat attatagtca actaatatga aataaggtta
     5161 agcataatgc gtgcacagat tagagcgttt tttgatccag cgacgtggac gatgagttac
     5221 gttgtttatg atgagcaggg tggattttgt gccattatcg acccagtgct ggattatgat
     5281 ccgaagtcag gaagaaccaa gacggtatca gctgataagc tgattgactt cgttcgggaa
     5341 aaccgattaa cggttgaatg gctccttgag acccatgctc atgcagacca tctttcagca
     5401 gccgcttatc tgcaagataa agtcggtgga aaaacggcga ttggtgagtc tattgccctt
     5461 gtgcaaggta gctttaagaa gctgtttaat ttgggtccag aatttgtagc agatggtttg
     5521 cacttcgatc accggatcac cgatggtgaa gtgcttcgcg taggaaaaat aacgcttaaa
     5581 gcaatatcgg ttccaggtca tacgcccgcg gatatggcgt atcagctcga tgacacagta
     5641 tttgtaggag ataccttgtt catgccggat gttggaactg cacgtgctga ttttccgggt
     5701 ggtgatgcgc atcagttgta ccattcaatt cagaaaattc tgagtctacc accgcagacc
     5761 cggttattta tgtgccatga ttacccaccc agcaatcgtt ctccgcagtg ggaaagtaca
     5821 gtgcttgagg agaaaatgca caatattcat atcaaagatg gcgtgacgga ggatgcgttt
     5881 gtcgcgatgc gaacgaagcg tgatgcaaca ttagaaatgc cagtgctgat gctaccttcg
     5941 gtgcaagtta atatcagggc aggccggttt cctgatcctg agtcgaatgg ggtttcctat
     6001 ttaaaaatac cgattaatgc aatttaattt gcgtagttaa aaaaagagga acagaaaatg
     6061 aaacgttttg atcatgccag aaggcaattc ttacaggcag ctacgcttgg ctcattggtt
     6121 tccttgcggc cagatttgct gcttgctgga gaaagccaga aaagcacaac gattaccagt
     6181 acctttaagc ctgacgtaga gatcgaattg ttcgcgcgca atgacaaggt ttcgatgatg
     6241 tcaggtgccg caacagatgt ttatcgctat catgggcagt tactgcaagg gccttccacg
     6301 acgttgtcaa cgatgccaag ttatcttggc ccaacgctaa atttggttca aggccagaaa
     6361 gtgcggattc attttcataa tcagctagct gagccctgca tcacccattg gcatggcatg
     6421 catgtaccac agcgcatgga tggccaccca atgtatgagg ttgcggaggg tggaacctat
     6481 atttatgaat tcgaggtcaa gaatcctgca ggaaccaact ggtatcatgc acacacacac
     6541 gagttgacgg cgaaacaagt ttatcgtggt cttgcgggac tgattgttat tagtgatgag
     6601 caagagcagc aattgcaatt gccaagcggc gaatatgatc taccgattgt gattcaagat
     6661 cggcgtttca cagaaagcaa tcaactctac tatcgccaac atggcatgca cgagcgcatg
     6721 gtaggattct tgggggatac gattttagtt aatgggcaga ttaatcctac tatgccagta
     6781 aaaacgcggg cctatcgatt gagagtattt aatggctcta attcaaggat ttataaattg
     6841 gcttgggagg atggcacacc gctcattgct attggaaccg atggtgcgtt gttagaaaag
     6901 ccggaaagct atccttatat catgttggcc cctgctgaac gtgttgaatt atgggtggat
     6961 ttttcaggac gtgaggtggg ttctgagctt gtgttgcaga gcttagcctt ccatggggca
     7021 gccccatcaa tggggatgcg tggaagaatg ggaggcggta tggaaggcga agcaatgggc
     7081 atgatgagtg gtctggcaca gggcgaacaa tttccgatcg ctaaattccg catcactgag
     7141 aaaatgagtg aatcacctca attgccgcaa actttgatca aaatgaatcg actgacagcg
     7201 cgcgatgcat caaatccaga tcgaacgatt ccgattgcga ttagcatggc gcatatgtcg
     7261 tggaaactca acggacgttc attcgaaatg gggaatgtaa tggatattga aaaaatccca
     7321 gtcaatacca ttcaaaaaat tagaattttt catgagagtc atcagatgca tggagggcgt
     7381 ggtggtatgg gtggtgggat gcgaggcggt cgaggaggag gtatgggcat gacgatgtcg
     7441 atggcgcacc cgattcacct tcatggtcag caatttcaaa tcctctcccg gcaattaaca
     7501 gaccaacagg caactggtgg ctacgatacg gtcaaacagg gctttattaa tagcggctgg
     7561 aaggatacgg tattggtgat gcctggcgag gaggttacaa ttattaagcc gtttgaagac
     7621 tatacgggtc tgtatctcta ccattgccat aaccttgagc atgaagatct cgggatgatg
     7681 cggaatttcc tcgtgagctg aaggtgaaca ctaaactgtg ggcgccattg agcttggcgt
     7741 tgattgcaca gtaaacgctt ttacaaacga agcgttagga aaagtgaggt atacttttcc
     7801 actttattta accttataag ttaatttcta gcacatgcta ctcattatga acgtattttt
     7861 taaatctcaa ttcaattgct ttggattatg cttagtttct ctcttgcttt ggacttcaat
     7921 cagttttgca aatagcgagg cagatttttt taaggcaacg ctaactggcg atggtgcaac
     7981 cgtagaaaaa ttattggctg aaggcttaga cattaatttg aaaaacccag atggatttac
     8041 ggcgttggct gtggcttctc agaaagggca tgacgccata gtgaaattac tactggataa
     8101 gaatgctgaa gtggatgcgg cgaatattca agggggaacc gcgttgttac tcgctgtaaa
     8161 aaatggccat gctgcgatcg tcgatactct acttgcaaag ggcgcagatc ccaatttgca
     8221 ggctaaaaat ggattaaccc cactcatcat cgcagccgca aaggattatg ctgatattgt
     8281 taaaagtttg gtggcaaaag gagcggatcc tgacttaagc gatactagtc aaacgacggc
     8341 gctgcatatt gcagcgcaga acggccatgt tgacattgta aacacgttgc ttacgaatgg
     8401 tgcaaaaccc gatcttcaag atgtgaatgg cgcttcagcc cttattttag cagctttggt
     8461 cggtgctcaa gaggtggttc aactgctact ggaaaaaggt gcgaaagctg acttgaaagc
     8521 gaccaatgat tttacagcgc ttatcttggc ggcacagaat ggtcagactg cagttgttga
     8581 aacgttgtta gcgcgaggag cgagtataaa tcagcaaaat caagatggaa ttacggcatt
     8641 gatgtgggct gcgttacacg atcatatcga cactgttaaa ttgttgattg agaagggcgc
     8701 tgataccaaa cttaaaagta aaagcgataa aactgcctta gatatggcaa aagatcaaac
     8761 gatcattgaa ttgctgaagt aagtgaagaa taaagtttat ggtgctaatg tgatgtgaat
     8821 tttgcaaaag taagcgaaaa tattctataa tagcaagcta actatggcag tttcatagtt
     8881 atatcccatc gttcctgacc ttccttcaat tttctttctt tggaaattgc ttatacccct
     8941 ggttagcagc gatgcattaa tcctgcgcta gcggtattgg ttgactcgtt caattcattc
     9001 ctgcttgctt ttttactcaa acttaatttg ggaaataacg tgtcttttga aaatttaaat
     9061 ttaaacccag caattattaa agcaattcat gaattaggct acactgcccc tacaccgatt
     9121 caacaacaag caattccaga attaatctta ggtcacgatg ttatggcttc tgcccaaaca
     9181 gggacaggta aaacagccgc ttttatgcta ccagcactga accggctagc tacaccgtct
     9241 aaaattagaa gccgcgggcc acgggtcctg gtattaacgc cgacacgcga gttagcactg
     9301 caagtttcag acgctaccag caaatttggt aaatatttgc cacacactaa agtggtgagt
     9361 attcttggcg gtatgtccta ccctttacaa aataaactgt tatcacaagt cgttgatatt
     9421 ttagttgcaa ccccaggaag acttattgat catattcagc gtggtcgtat cgatttttcc
     9481 cgcttggaaa tgctggtgtt ggatgaagca gatcgaatgt tagatatggg atttatcgat
     9541 gatgttgaag ccatcgcctc ccgcacacca gatacacgtc aaaccttatt attttcagca
     9601 acgttggatt catcgattga taagatcgca ttacgattat tgaaatcacc taaacgtatt
     9661 caggtagccg ctcaacacgc aaagctggat aatattgagc agaaactcca ttatgttgat
     9721 gatctttcgc ataaaaatcg attactagat catttgttac gtgatgagtc acttaaacaa
     9781 gctatcgttt ttaccgcaac caaacgagat gcggatgcat tggctgatga tttggctgta
     9841 tccggtcatg aagtagcagc attacatggt gacatgaatc agcgtgaacg aacgagaaca
     9901 ctgacgcgat taagagcggg gcgtttaaga atactggttg ctactgacgt ggctgcccgt
     9961 gggatcgatg tcacgaatat tacccatgtc attaattttg atttacccaa atttgcagaa
    10021 gattatgtac atcggattgg tcgaaccgga cgagccggag catccggggt agcagtatcg
    10081 tttgcaactg gaagggacag tatcagcctg agaaaaatag agcgatttac cgggcagacg
    10141 attgctcctt tcgtcgttcc aggaatggag tcaaaatata aaccaaagac tggtggtggg
    10201 cgttcacac
//
LOCUS       DAOQKQ010000094        10187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_3108_length_10187_cov_6.167687, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000094 DAOQKQ010000000
VERSION     DAOQKQ010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 10187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10187
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3108_length_10187_cov_6.167687"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..689)
                     /gene="cysC"
                     /locus_tag="PKZ49_07455"
     CDS             complement(63..689)
                     /gene="cysC"
                     /locus_tag="PKZ49_07455"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HND36436.1"
                     /translation="MTTLPRVSSDILWHHASVTRHRRELLNGHRGAILWFTGFSGAGK
                     STLAHAVEEVLHQSGYKTFVLDGDNVRHGLCNDLGFSPENRVENIRRVGEVAKLFMEA
                     GIIVLTAFISPFRVERERVRNLVAQGDFIEIYCNSPIEICESRDVKGLYKKARAGQIA
                     EFTGISSPYEAPENPELTVNTSITDLNTSVQQVINELQSRNIIKKINE"
     gene            complement(731..2275)
                     /locus_tag="PKZ49_07460"
     CDS             complement(731..2275)
                     /locus_tag="PKZ49_07460"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HND36437.1"
                     /translation="MIQMILLLILTTLLAAISIRFAIIYALANNIADHPGGHKQHDTV
                     TPFVGGIGLLIAFSIALIVLFHNQPEFWIKWLALGIAAAIVFIIGLLDDIFHLNYKIR
                     FLAQGIVALIMIFMGGVQLSELGTLISNNQVLELGLFAIPLTMFATIGGINALNMIDG
                     IDGLAGTLSWITLLLISILAFFGGDQANTTLAIALVGGISGFLYFNLRYSTQNRARVF
                     LGDNGSMLLGFLFAWLLVDLSQGSHASMTPVTAIWLFAVPLMDTVTVMLRRIWLRQSP
                     FVPDHNHLHHILLHAGFRIEDTVFVIGTLHLILGSFGLVGLYLGIPESIMFFSFLVIY
                     TGYFYLTQHPWYIMSSLRHLHNFLGLLPTDDYGVFLGSYSAREAETLVKMVCKELGPS
                     VDSWVHVIEQQSSNNYAEKRYSVAVNIRLSNTDYVTDKEISQYIAKLSHRMMERWGIR
                     VRQFVKRNIKNDRRIKDIGNPLGGNFRVSERRSIEIPILMFEAMFDKLAIERNNHSEE
                     SISETR"
     gene            complement(2672..3286)
                     /locus_tag="PKZ49_07465"
     CDS             complement(2672..3286)
                     /locus_tag="PKZ49_07465"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HND36438.1"
                     /translation="MPDTIVDLIRHGEPAGGQRYRGHRVDDPLTEKGWSQMWNSVGEY
                     QKWTHIITSPLQRCNAFALSLGKRLNIPVIVESRFKEVGFGLWEGLSHDEIRNNRAEE
                     FQAFITDPVNCRPQGAEPLDTFLQRVNLAYEDIIKLNQNHHYLIVAHAGVIRAIIART
                     VHLAPSGFYRIKISNGGVTRIRHTTTGPILELLNGKLAGNHSND"
     gene            3631..4608
                     /locus_tag="PKZ49_07470"
     CDS             3631..4608
                     /locus_tag="PKZ49_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HND36439.1"
                     /translation="MKKLIIICGMPRSGTTWLGKIFDSHPGILYRHEPDAGGKLNLLP
                     LFTETWVEEELKFIKQFISELESHTRVNQVSKFPIFKKIRESDIHFQVRKAYLTALKI
                     ASRYFGNFSAYEMLPNQSDMTIVWKSIESLGRMPIMAQAVQELGIEAYFIHIIRHPCG
                     QLNSTLRGQAKQVFTGNYNAAEDEPLFKLLASSSQAQLYELTVSQFQQMNAEQRLTWR
                     WALMNDFALRLMENKPNYFKVRYEDICQEPELTTKELFTFCKLGWNEQTAHFLEMSTK
                     QDVPDYYSVIKNPLNSANAWRKKLSEQQINDIFAVSEKTLSGKLYLPRD"
     gene            complement(4646..5551)
                     /locus_tag="PKZ49_07475"
     CDS             complement(4646..5551)
                     /locus_tag="PKZ49_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HND36440.1"
                     /translation="MATANLLALLDDIASVLDDIAVLSKLAAKKTAGVLGDDLALNAQ
                     QVMGIQANREIPVVWAVAKGSFWNKAILVPAALLISALLPWLVTPLLMIGGAYLCYEG
                     FEKLAHRFLHDAVEETTHKKALSKAIADPAIDLVAYEREKIRGAIRTDFILSAEIIVI
                     ALGIVANETLMTRIGVLIAIALVMTIAVYGLVAVIIKLDDLGLWLKKKSDATLTIIGQ
                     FLLNATPWLMRFLSVAGTVAMFLVGGNILMHGIPSFAHWIEPIISSTNGLSTLTISSL
                     FNILLGIMCGGLILGITRVIQKIKS"
     gene            complement(5663..6397)
                     /locus_tag="PKZ49_07480"
     CDS             complement(5663..6397)
                     /locus_tag="PKZ49_07480"
                     /inference="COORDINATES: protein motif:HMM:NF016291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HND36441.1"
                     /translation="MSYGDIVGRLIKVLANEGTSKLEHIIKSSQQSQTASGVWDFVLN
                     FFRTKQAGNMTGSGIGSLGVIAGALLNGGAAAVKGAMGGGVLAMLGTMAINALQKRLN
                     IQSADIESGLLSKFLSNEDIASMSSAETEKLVLSAMINAAKADGQLDQQEMDKILGKA
                     GVDGISDDEKKFIQDELNKPFDLQNLVSAVPNQVVGAQVYAASLFAIDINTDAEKDYL
                     KNLAQALELDGETIARLHEMTGAPLV"
     gene            6833..7270
                     /locus_tag="PKZ49_07485"
     CDS             6833..7270
                     /locus_tag="PKZ49_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HND36442.1"
                     /translation="MAITRYEPWGLLTQLQKELEQMRSDVAGDGSSATAEWAPAVDIK
                     EESDRFVLHADLPGVKPEEIDVSMENGVLTIRGEKKTEAKTEKEGYKRIERTYGSFYR
                     RFSLPDTANPDAISAVSKNGVLEITIPKRESVQPKKISVAAVE"
     gene            7440..9356
                     /locus_tag="PKZ49_07490"
     CDS             7440..9356
                     /locus_tag="PKZ49_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HND36443.1"
                     /translation="MPLITLEDSCLAFGHHALLNHVDLQLDPGERIGLIGRNGSGKSS
                     LLKVLAKEIKLDDGKLWHKPELKLVYVSQEPEFGTAGTVYHEVSRGLGHLSQVLNEYH
                     EISQALSEGKGSNETNFSRLASLQNFLELNDGWRIQTRVETVIHQLGLSAQDLIGNLS
                     GGTRKRVALARALVVLPDVLLLDEPTNHLDFTSIEWLEELLQTFAGSVIFITHDRRFL
                     DNVSTRIIELDRGLLGSFTGNFAHYLQKKMEMLQSEATQQHKFDKVLAQEEVWIRKGV
                     QARCTRNEGRVRRLEALRLERAARRTRVGSVNFNVEVGERSGHLVAELQQVTKCFGDK
                     TVIKDFSCRILRGDRIGLLGPNGSGKSTLLKLILGELEPDLGEIKRGTKLSIAYFDQM
                     RGQLNPESTVIEAINQGSDFIEINGERKHVISYLEDFLFAPQRARSPVKSLSGGERNR
                     LLLARLFTQPANLLVLDEPTNDLDIETLELLEVLLQSYTGTLFLVSHDRTFLDNVVTQ
                     VIAFEGDGLLNEYVGGYEDWARSKSAKDNLTQLLLQTSKISVPTEEKKSRLSPKLSYH
                     EVRELAALPEKIDSLEREQTLINQQLSDATLYKSNPEQIKVLQTRLTKIEEQLTQFFS
                     RWEELEAKQSAAKM"
     gene            9456..9788
                     /locus_tag="PKZ49_07495"
     CDS             9456..9788
                     /locus_tag="PKZ49_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HND36444.1"
                     /translation="MHKVKKLAVKLAFAVGLALAISGGVHAASDAEGVKSKTSMCEGC
                     HGIEGYRTAYPVAYHVPKLGGQHAVYLAKALRDYKAGERNHPTMKAIAATLSDKDIEE
                     LAAYYAGN"
     gene            9818..10132
                     /locus_tag="PKZ49_07500"
     CDS             9818..10132
                     /locus_tag="PKZ49_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HND36445.1"
                     /translation="MKNYFIAAVTGAIFVISGNAIAADIEAGKRKAAEVCASCHGADG
                     NSPAPNFPKIGGQPRTYLEKALKDYKTGDRKDPVMAGMAAALTPDDIGNLAFYYSSQN
                     IG"
BASE COUNT         3151 a         1930 c         2154 g         2952 t
ORIGIN      
        1 attgatcttg ctatataaga tgaaaaacac tgagaatagt tcataggcat tttttagaaa
       61 gactattcgt taattttttt aatgatattc cttgattgca attcattaat gacttgttga
      121 acggaagtat tcagatcggt aatgcttgta ttaacagtca gctctggatt ctctggtgct
      181 tcatagggag aagaaatacc tgtaaattct gcgatttgac ctgctctggc ttttttatag
      241 agccctttaa catctcgaga ttcacaaatt tcaattgggc tattgcaata aatttcaata
      301 aaatcacctt gcgcaactag atttcttact cgctctcgtt ctacgcgaaa tggggaaata
      361 aaagcagtta gcacgataat tcctgcttcc atgaatagtt ttgcgacttc acctactcgg
      421 cggatatttt ctacgcgatt ttcaggagag aatccaagat cgttgcataa cccatgccga
      481 acattatcgc cgtcgagtac aaacgtttta tacccgcttt gatgaagcac ttcttcaaca
      541 gcatgggcca aggtagactt tccagcgcca gaaaacccag taaaccagag aatagccccg
      601 cgatggccat tcagtaattc tcgtcggtga cgagttactg aagcatggtg ccagagaata
      661 tcgcttgata ctcggggtaa cgttgtcatg attactcctg aaatcgttga aatgttatag
      721 cttagcttcc ttagcgtgtt tctgaaattg attcttctga atgattgttg cgctctatcg
      781 caagtttgtc gaacattgct tcaaacatca gtatcgggat ctcaattgaa cgtctctcag
      841 aaacacgaaa gttgccgccc aaaggattgc cgatatcttt gattcttcgg tcatttttga
      901 tattacgttt aacaaattgc cggacacgaa tgccccagcg ttccatcatg cggtgtgaca
      961 gttttgcaat atactgacta atttctttat cagtgacata atctgtgttc gataatcgga
     1021 tattaactgc gactgaatat cttttttcag cataattatt tgatgattgt tgttcaatga
     1081 catgcaccca ggaatcaacg cttggtccaa gttctttaca gaccatttta actaatgttt
     1141 cagcttctcg cgcagaataa ctacccaaga aaacaccgta atcatcagtg gggagtaacc
     1201 ctaagaaatt gtgtagatgg cgtaacgatg acatgatata ccatggatgc tgtgttaagt
     1261 aaaaatagcc ggtgtatatc actaagaaac taaagaacat aatagattcg ggaataccca
     1321 gatacaaacc cactaagcca aatgatccta gtattaagtg aagcgtgcca ataacaaaaa
     1381 cggtatcttc tatcctaaaa cctgcgtgta gcaggatatg atgtaaatga ttatgatcag
     1441 gaacaaatgg agattggcgt aaccaaattc tgcgcagcat cacagttacg gtatccataa
     1501 gcggaacagc aaatagccaa attgctgtta caggagtcat ggaggcatga gatccttgtg
     1561 aaagatcgac caatagccaa gcaaataaaa aaccaaggag catactcccg ttatccccaa
     1621 gaaagacacg agcacgattc tgtgtggagt agcggaggtt aaaatataag aaaccagaaa
     1681 tacctccaac taatgcgata gcaagcgtgg tgttggcctg atctccccca aaaaacgcca
     1741 agatactgat cagtagtaaa gtaatccatg atagcgtacc agcaagtcca tcaatgccat
     1801 cgatcatatt gagtgcattg attcctccga tagtagcaaa cattgtaagg ggaatggcaa
     1861 atagacctaa ttccagaact tggttattag aaattaatgt tccaagctcg ctgagttgta
     1921 ctcctcccat aaagatcatg atcagcgcga ctattccttg agcaagaaat cttattttat
     1981 agttgagatg aaagatatca tcaaggagac ctataatgaa aacgatagcc gcagctattc
     2041 caagtgctaa ccattttatc cagaactctg gttgattatg gaataaaacg attaaagcaa
     2101 tagaaaatgc aatgagtaac cctattccgc cgacaaaagg ggtcacggta tcatgttgct
     2161 tgtggccacc cgggtgatca gcgatgttat ttgctagtgc ataaattatt gcaaatcgaa
     2221 tactaatcgc tgctaataaa gttgttagta tgagcaaaag aatcatttgt atcataatca
     2281 gattacccct taagaattga tagatttcta atcagattgg gggaagaact tagcatagat
     2341 aaatgaagga aatttgaatc cttaaaacgc acccgatcat tttataatat tatcaataag
     2401 atagcattat aaatttcttt agcatacaac aaatgattga aataattata ttaattccta
     2461 tcagtaagga tatcaattta cgctattctg atgctatcga aagaagttaa gccaaagtaa
     2521 tggaaactag aattttgtat ttttacatat accgcaaaat cagagaatgc ggcaaattgt
     2581 cacaccccct agagcaagtg aataaactga atgccgcgat aaacttttag taatgagagc
     2641 gatggagcta gtattaaatg actaatattc atcagtcatt tgaatgattg cctgcgagtt
     2701 tcccatttaa taattctaga atcgggcctg ttgtagtgtg acgaatgcgt gtaacgccac
     2761 cattactaat ttttattcga tagaaacctg aaggtgcgag atgcacggtt cttgcaatga
     2821 ttgcacggat aacccctgca tgggctacga tgagatagtg atgattttga ttaagtttaa
     2881 taatatcctc ataggctaaa ttgacccgtt gcagaaaagt atctagaggt tcagcgcctt
     2941 gcggcctaca attgacaggg tctgtgataa atgcctgaaa ttcttctgcg cgattatttc
     3001 tgatttcgtc atgactcaat ccttcccaaa gtccaaagcc aacttcttta aagcgtgatt
     3061 cgacaataac cggtatgtta agtcgcttac ctaatgaaag agcaaacgca ttacagcgct
     3121 gtaatggaga cgtaataata tgtgtccatt tctgatattc tccaacggaa ttccacatct
     3181 gtgaccaacc tttttccgtc agtggatcat cgacacgatg accacggtaa cgttgacctc
     3241 ctgctggctc gccatggcga atcaaatcaa caatggtatc aggcattaac ataatcggta
     3301 ctcgtaatgt ggcgtaaatt aaatagcgct aaatttttgt atgcttttat ttgcaatcta
     3361 tcataaagaa atattgaact gaagcaattg agtgagaagc aatttccttt taaaggagca
     3421 gccaggtaac ctgtaaaaaa taatgtttac tttttagagg cttgatttgc agatgtgtta
     3481 agtgtttaaa aaggcgttac tcttgtggtt tttatcattc tataaagaaa aactgtcgtg
     3541 tatgatgcat gtggccctaa agcaagccat gccaaatagt tgagtactga ttgatatatt
     3601 gatgctaaat catcccgaat aggctagtta atgaaaaaat tgattattat ctgcggaatg
     3661 ccgcgctcag ggacaacctg gctaggtaag atatttgata gccaccctgg aattttatat
     3721 cggcatgaac ccgatgccgg cggaaagcta aatttattac cactctttac tgaaacctgg
     3781 gttgaggaag aacttaaatt cattaaacaa ttcatttccg agcttgaatc gcacacgcgg
     3841 gttaatcaag taagcaagtt tccaatcttc aagaaaattc gtgaaagtga tatccatttt
     3901 caagtgcgta aagcctatct cacggcatta aagattgctt cacgatattt tggtaatttt
     3961 tcagcttatg aaatgctacc taatcaatcg gatatgacga ttgtttggaa aagtattgaa
     4021 tcgctggggc gaatgcctat aatggcgcaa gctgtccaag aactaggaat tgaagcctat
     4081 tttattcaca tcattcgtca tccgtgcggg caactaaact caacattaag aggtcaagca
     4141 aagcaggtat ttacagggaa ttataatgct gctgaagatg agccgctttt taaattgctt
     4201 gcaagttcca gtcaagccca gttgtacgag ctaacagtgt cccaatttca acaaatgaat
     4261 gcggagcaac gtttaacgtg gagatgggcg ctcatgaatg attttgcgtt gcgtttaatg
     4321 gaaaataaac cgaattattt taaggttcga tatgaagata tctgccagga gcctgagctg
     4381 actacgaagg agttatttac attttgtaaa ttgggatgga atgagcaaac agcccacttt
     4441 cttgaaatga gcaccaagca ggatgtgcct gattactata gtgttatcaa aaatccactt
     4501 aattccgcaa atgcctggcg aaaaaaattg agtgagcagc agattaacga tatttttgcg
     4561 gtatctgaaa aaacgttgag cgggaaactc tacttgccga gagattaaat tttgttagtg
     4621 aattgtaatt agctaggttg tttttttatg atttgatttt ctgaatgacg cgtgtgattc
     4681 ctaatataag accaccacac ataatgccta atagaatatt gaatagtgat gagatggtta
     4741 aagtagatag accattggtc gaggaaataa tgggttcaat ccagtgagcg aaactgggta
     4801 ttccatgcat cagaatattg ccacccacta gaaacatcgc tacggttcca gcgaccgata
     4861 aaaaccgcat taaccaaggt gttgcattga gaagaaactg cccgatgatg gtgagtgtag
     4921 cgtcgctctt tttctttagc cagagcccaa gatcatctaa tttaataatg actgcaacta
     4981 acccataaac cgcaatcgtc atgaccaatg cgatggcgat tagtacgccg atacgtgtca
     5041 tgagcgtctc attcgcgacg atacctaacg caatcactat tatttcagct gacagaataa
     5101 aatcggtacg gatagctccc ctgatctttt cgcgctcata ggcaactaaa tcaatagcag
     5161 ggtcagcgat agctttggat aatgcttttt tatgcgttgt ttcctctact gcatcatgca
     5221 gaaatcggtg tgcaagcttc tcaaaacctt cgtagcaaag ataagcaccg ccaatcatca
     5281 gaagtggagt aactaaccaa gggagtaaag cgctaatcag taacgctgca ggaactaaga
     5341 ttgccttatt ccagaatgag ccttttgcaa ctgcccacac cacgggtatt tcacggttag
     5401 cttgaatgcc catgacttgc tgcgcattaa gcgcaaggtc atctcccagt acccccgctg
     5461 tttttttagc ggcgagctta cttagcacag caatatcgtc taaaacactc gcaatatcat
     5521 cgaggagtgc gagaaggttt gcagttgcca tcgaggttaa tcaataggat ttcaaaaaag
     5581 cttaaatttt ggtaacgata tcagataaac acctcgctat cgttagcgag gcgtcaaaca
     5641 ttcctggtta tacaagaatt atttatacca gtggcgcacc ggtcatttca tgcagacgtg
     5701 caatcgtttc accatccaac tcaagggcct gagctagatt ttttaaataa tctttttctg
     5761 catcagtgtt aatatcgatt gcaaacaaag atgccgcata aacttgcgct ccgacaactt
     5821 gattaggaac agcagatact agattttgta aatcgaaagg tttattcaat tcgtcttgaa
     5881 tgaatttttt ttcatcatca gaaattccat caaccccagc tttcccaaga attttatcca
     5941 tttcttgttg atcaagttgc ccatcagctt tagcagcatt gatcattgcg ctgagtacta
     6001 gtttttctgt ttcagcagag ctcatcgatg caatatcttc attcgataag aattttgaca
     6061 ataatcccga ttcgatatct gcggattgga tgtttaatct tttctggagc gcattgattg
     6121 ccatggtacc cagcatagct aagacgcctc cacccatggc tccttttaca gcagcagcac
     6181 ctccatttaa tagggcgcca gcgataaccc ctaagctacc aattccgctg ccagtcatgt
     6241 tgccagcttg ttttgttcga aaaaaattca acacaaaatc ccatacgcca cttgcggttt
     6301 gggactgttg agaagatttg atgatgtgtt ctagctttga ggttccttca ttcgctaata
     6361 cttttattaa gcgaccaaca atatctccat aactcatttt ttttcctttc tgtgaattga
     6421 gatggcttta tctatagcaa ttgcatgaga taaagatgaa aaggtttcct atgatttgtg
     6481 atacgtgcca gggtaatcat accagcattt tgggaagtta tagcgcaact cagtgaatga
     6541 agataaagct tcactataag aatttctttt aaatgagttg actagattgc cagcacttga
     6601 gttatgcggt gcatagcaat cctatgataa tgagggatta aaaaaatcat tttatctgct
     6661 tgaaaatggt aggcgcatcc ctatattaca agcagtactc gttattgcat acgatcgttg
     6721 atgatgagta tttggagatg ctcagatatg ttcaatggca ctatagcatc aggatatata
     6781 ggaaatttta attgcaggta atttgcatat ttataattgg agataatgaa ttatggctat
     6841 tacacgttat gaaccatggg gattattaac acagcttcaa aaagagttgg agcaaatgcg
     6901 gagtgatgtg gctggcgatg gctcttctgc taccgcagaa tgggcgcctg ctgtcgatat
     6961 caaagaagag tcagatcggt ttgttttaca tgctgatctg ccgggggtaa aaccagaaga
     7021 aatcgatgtc agtatggaaa atggtgtatt gaccatcaga ggcgagaaaa aaactgaagc
     7081 aaaaactgag aaagaaggct ataaacgaat tgaacggact tacggttcat tttataggcg
     7141 ttttagcctg cctgatacag caaatccaga tgcaatttca gctgtttcta aaaatggcgt
     7201 actggaaatt accattccaa aacgtgaatc ggtacagcct aagaaaatca gtgtggcagc
     7261 agtagaataa ttaagtttat tacttttagt aaattaaaaa gggaatctaa tgattccctt
     7321 tttcttttga attaaacgat tcaagataat aagcaatcag cgaactagtt tgctgcatcc
     7381 tataattcat gaaaaaatcg caccatctta tttgatgttg attatttgta cttttactta
     7441 tgccacttat tacccttgag gatagctgct tagcctttgg ccatcacgcc ttactgaatc
     7501 atgttgatct ccaattagac ccaggagaac gaatcggctt gattggacgc aatggcagtg
     7561 gcaaatcaag tttgttaaaa gtactagcga aagaaattaa gcttgacgat ggaaagcttt
     7621 ggcataaacc agaactgaag cttgtttatg tttcgcagga gcctgaattt gggacagcag
     7681 gtactgtcta tcatgaagtt tctcgagggt tgggtcatct gagccaagtt ctcaatgaat
     7741 accatgaaat ttcccaagcg ctgagtgaag gcaagggaag caatgaaacc aacttttctc
     7801 gactcgcatc attgcagaat tttttggagt taaacgatgg atggcggatc caaacgagag
     7861 ttgaaaccgt cattcaccaa ttaggattat cagcacaaga tctgattgga aatttatcag
     7921 gtggaacccg taaaagagtc gcgttagcgc gtgctttagt cgtgttgcct gatgtattgt
     7981 tattggatga acctactaac catcttgatt ttacttcgat tgaatggcta gaagaattac
     8041 tccagacttt tgcaggcagt gtgattttta tcactcatga tcggcgcttt ttagataacg
     8101 tttctactcg aattattgag ctcgatcgcg gattacttgg gagttttaca ggtaattttg
     8161 cgcattacct gcaaaagaaa atggaaatgt tgcaaagtga agcgacacaa cagcacaaat
     8221 tcgataaggt gcttgcgcaa gaagaggtat ggatacgaaa aggtgtacaa gcgcgttgta
     8281 cgcgtaatga agggcgagta agacgtttgg aagcattgcg tttggaacgc gcggcacgtc
     8341 gcacccgtgt tgggtctgtc aatttcaatg ttgaagtagg tgagcgatca ggacatttag
     8401 tagcagaact tcagcaggtt accaaatgtt ttggtgataa aaccgtgata aaggattttt
     8461 cttgtcgcat tcttcgtggc gatcgaatcg gtttattagg ccctaatggt tcgggtaaat
     8521 cgaccttact aaaacttatt ctaggcgaat tggaacctga tttaggggaa ataaagcgag
     8581 gaacgaaatt atccattgct tattttgatc aaatgcgagg tcaattaaat ccagaatcca
     8641 cagttatcga ggcgattaat caaggctctg attttatcga gattaatggt gagcgtaagc
     8701 atgttatcag ttatcttgaa gattttcttt ttgcaccgca gcgagcgcgt tcaccggtta
     8761 aatcattatc cggtggggag cgtaatcgtt tgttattggc acgtctgttc acccaacccg
     8821 ctaatttgct cgtattagat gagcctacga atgatcttga tatcgagacg ctggaattat
     8881 tggaagtttt attgcagagt tacaccggaa ccctgttttt agtcagccat gatcgtactt
     8941 ttctagataa tgtggttaca caagttattg cgtttgaggg agatggattg ctaaacgagt
     9001 atgttggtgg ttatgaagat tgggcacgaa gtaaaagtgc caaagataac ttgacccagt
     9061 tacttcttca aacaagtaaa atttctgtcc cgactgaaga aaagaaatca agattatcgc
     9121 ccaaattgag ttatcacgaa gtgcgggagc tagcggcgct accagaaaag attgattcat
     9181 tagagcgtga acaaactttg attaatcagc aactcagtga tgcaacactc tataaaagta
     9241 accctgaaca aattaaggta ttgcaaacgc gtttgaccaa gattgaagag cagctcactc
     9301 aattcttttc gcgctgggag gaattagagg ctaaacaatc cgcagcaaaa atgtagggat
     9361 tgagcgtttt ttccctataa acaacaggga gatagcgatt atcttcattg actaaacagc
     9421 ccccatcgtt taaagttctg atttttgaga ggaggatgca taaagtgaaa aagttggccg
     9481 ttaaattagc gtttgcagtc ggcttggctt tggcaatttc tggtggggtt catgcggcca
     9541 gtgatgctga aggagtgaaa agtaaaactt ctatgtgcga aggatgccat ggaatagagg
     9601 gttaccgtac tgcttatcca gttgcttatc atgtaccaaa attgggtggg cagcatgctg
     9661 tgtacttagc aaaagcattg agagactata aagcgggtga gcgtaatcat cctacaatga
     9721 aagcgattgc agctaccctt tctgataagg atatagaaga attagctgca tactatgcag
     9781 gtaactaatt tcccatttac gcaaggataa taagattatg aagaattatt ttattgctgc
     9841 agtaaccgga gcaatttttg ttatttcggg aaatgcgatt gcagcagata ttgaagcagg
     9901 taaaagaaaa gccgctgaag tatgtgcttc ttgccatgga gcagatggca atagcccagc
     9961 gcctaatttt cctaaaattg gcggtcaacc aagaacttat cttgaaaaag cactaaaaga
    10021 ctataaaaca ggcgatcgca aagatccagt tatggcagga atggcagcag cgttgactcc
    10081 agatgatatc ggcaatctcg ctttttacta ctcaagtcaa aatattggct agacaatgaa
    10141 aaagtaagtg tattagatca ataaagtagg gcattcatta gtgcgct
//
LOCUS       DAOQKQ010000095         9984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_3184_length_9984_cov_5.473864, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000095 DAOQKQ010000000
VERSION     DAOQKQ010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 9984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9984
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3184_length_9984_cov_5.473864"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(298..1698)
                     /locus_tag="PKZ49_07505"
     CDS             complement(298..1698)
                     /locus_tag="PKZ49_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HND36446.1"
                     /translation="MSMLLGNVSLQASEKITTSQDQKSITVTIYNENLALIKDSRNVE
                     LSQNINQLAWREVSALIRPETALLGNQTSQSGFHVFEQNFDFDLLTPQKLLEKHVGKE
                     ITVVRTNLATGTETNETAVVLSTNEGIVLKFNNRIETGAPGRMVFPGVPENLRDKPTL
                     LLLLHSPESGKHELELSYLTGGLSWRADYVAVLSENDSKLDLNGLVTLTNQSGVAYPQ
                     ARLQLVAGDVNQIQSEQRIGRKMAAMSVEMADAAQMKEESLFEYHLYTLQRPTTLAEN
                     QTKQVALMSASQIPVNKIFFLQGADYYYSGKYGVLGQKLKVSVLISFDNKGEQLGIPL
                     PKGIVRVYKKDVQGYSQFIGEDQIDHTPKNEQIKLKLGSAFDITADKIQTDFQQIAGS
                     IHQTSIFETTYQITIRNAKKESVVVQVQEPIPGDWVIVSESIKHKKISANTVEWKIPV
                     SAEGEATFVYRVRVKY"
     gene            complement(1748..3898)
                     /locus_tag="PKZ49_07510"
     CDS             complement(1748..3898)
                     /locus_tag="PKZ49_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36447.1"
                     /translation="MTFKSRLMKSILNIQSPHVDSDSDSNVVLLNPSEKLNGDLTYKH
                     CVDSENYELLSAGNSIPPQLSAETAFQEAKKRIEADAKASVAAEARARAEASARLAAE
                     LRIKAETAATEEARARAKAEALASKEAQARKELEAKLRQTIEDGIKAEKEIVATLRAK
                     VQAETLIAEKTRVRAKEEALILDQIKAKEEAEERAIEAAIVRRKIEEEARLLALSATE
                     AEAKAAAMARVKIQEEEKLLALAHAKTETERQILDEVRLRAEVEAKALAKAQEHLTAE
                     TKSREAEEAKLYAEIQAEQVTQAKIDAELNAKTAAELRAEADAKAAEAAIARAEADKA
                     AAANAEATAKAQLEAEAIAISRAQAEARLLEEINQKITAEKEAQFSAESRAQAEQQEV
                     ATLSAKMNADNAAVEATKERIKAQAEAIKKSRERAIAEATAKQAAEERMAAEAKAAAE
                     VEDRAKVDKLAAEAAIELAEIEARLAADARKRHDAIVQAKISITSRIENEQRMTELAS
                     KIAQARIVAAVKTEEKLKATEIAASTTFARVQAETSALNAIQARIESDALAIEQAIAR
                     EAVEAMAKEAADARTKADQRAIAQASQQIQQDLAETEIIRNNQSAEETTEEQATFSQE
                     DYVGSPLIEQYEEEESSELSDENEYDEEFRADIQDLADSPIEDSTELVEYVEINEIDF
                     GWQTELDSESTTNDNDSSASDEELEEDNRGKIFS"
     gene            complement(4072..4875)
                     /gene="fliR"
                     /locus_tag="PKZ49_07515"
     CDS             complement(4072..4875)
                     /gene="fliR"
                     /locus_tag="PKZ49_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HND36448.1"
                     /translation="MISFTYAELSTLLASFFWPLSRILAFISSAPILSHRSIPIQVKI
                     GFALLLTLTIVPTLQTLPSVDPGSEIGFLILMQQIVIGLAMGFAMRVIFVAIEMAGEI
                     IGLQMGLGFALFFDPQNSGQVQLIGRFLGLIALLVFLAIDGHLQMIAILAQSFSVLPI
                     GTAGMPSLSFSILANWGSEIFLLGMKLSLPILTTLLIANLALGILTRAAPQLNIFAVG
                     FPLTLAIGLAMMSLVLPYYLPILEQMFQNGLQIMLEIITPSTIEPSPTE"
     gene            complement(4909..5181)
                     /gene="fliQ"
                     /locus_tag="PKZ49_07520"
     CDS             complement(4909..5181)
                     /gene="fliQ"
                     /locus_tag="PKZ49_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HND36449.1"
                     /translation="MSPESAITIGRHALEITFMVSAPLLLAALVTGLIVSIFQAATQI
                     NEMTLSFIPKLLIMFAVMVLAGPWMLSVMMDYVQRLYANIPWLTTG"
     gene            complement(5194..5973)
                     /gene="fliP"
                     /locus_tag="PKZ49_07525"
     CDS             complement(5194..5973)
                     /gene="fliP"
                     /locus_tag="PKZ49_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635079.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HND36450.1"
                     /translation="MTDKRQAIVTWHRSLLFLLLITLCLMVLPVYAQQSNFPAFTSTP
                     TQNGGTAYTLSIQTLLLFTTLTFIPALILMMSSFTRIIIVLSLLRLALGTQSSPPNQV
                     LIGLTLFLTLFVMSPVIDRVYSEAYVPFSQDKINLFEAGEKASVPLKAFMLRQTREAD
                     LALFTQISNSEEIESRDQVPLKILVPAYITSELKTAFQIGFIVFIPFLIIDMVVASVL
                     MAMGMMMLSPMIISLPFKLMLFVLVDGWHLLIGSLTQSFYT"
     gene            complement(6001..6414)
                     /gene="fliO"
                     /locus_tag="PKZ49_07530"
     CDS             complement(6001..6414)
                     /gene="fliO"
                     /locus_tag="PKZ49_07530"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HND36451.1"
                     /translation="MNLQVPLRAEEVAKPEIAAMPSLVTGSSVLHMFLALLLVLAVIG
                     VIAWLLKRFGIGPGNQSNLIKVVAAAAVGQRERIVIVEIANAWLVLGVAPGHINLLHQ
                     IEKIASSTGSEQLSTEGTNDFFAQLQKNIKKQQDE"
     gene            complement(6450..6908)
                     /gene="fliN"
                     /locus_tag="PKZ49_07535"
     CDS             complement(6450..6908)
                     /gene="fliN"
                     /locus_tag="PKZ49_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HND36452.1"
                     /translation="MSETEQTEKPIEADDWAQAMEEQSSTASDPAAIPESNHQSISSD
                     TLTASTPVFEQFSKDNVLNNAKNDIDIILDIPVQLTVELGRTRIAIRNLLQLAQGSIV
                     ELDGMAGEPMDVLVNGCLIAQGEIVVVNEKFGIRLTDIITPSERIRKLNR"
     gene            complement(6912..7919)
                     /gene="fliM"
                     /locus_tag="PKZ49_07540"
     CDS             complement(6912..7919)
                     /gene="fliM"
                     /locus_tag="PKZ49_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HND36453.1"
                     /translation="MAENFLSQDEVDALLSQAGIGDDKPEEASATQRESSTAPEGIRP
                     YNMATQERIVRGRMPTYEIINARFARLLQIGLYNFMHRAADISPGTMKTVKYSEFIRS
                     LVVPTNMNLVSMKPLRGTALIVFDPKLIFLIIDNLFGGDGRFHWRVEGREFTLTEQRI
                     ISRLLEVVYEDYQKSWKSVYPIDVEQVRTEMNPQFASIATPGEIVIATTFTVDIGGTG
                     GEFHTCIPYTMLEPIRDTLSSTLQGDQLELDKRWVRSLTKQVQSAEVELIANIGHAKI
                     TLNQILNMQEGDVVALDIPDTVTALADGVPLLECQYGVLNGQYALKVKSILSHSENST
                     E"
     gene            complement(7933..8436)
                     /gene="fliL"
                     /locus_tag="PKZ49_07545"
     CDS             complement(7933..8436)
                     /gene="fliL"
                     /locus_tag="PKZ49_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HND36454.1"
                     /translation="MSASAEAGSPAKESTGGKKKMIMLILVAILSIGAGVGGTWYFMQ
                     SQQGEEDEEPKKKKKKKPTEFIKFEPFTVNLQPEEDGERHYLQVEISLKVNQTEVVGI
                     IKEKMPEIRNKALLVLSSKKASEIFTLEGKQQLGKVIVEEIQLIVGDEDFNEDIKETL
                     FNSFIIQ"
     gene            complement(8645..9442)
                     /locus_tag="PKZ49_07550"
     CDS             complement(8645..9442)
                     /locus_tag="PKZ49_07550"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HND36455.1"
                     /translation="MSKFDLTQQFQNILKLVPQPRKKSGFKVALVCIAVALILTLDYY
                     LGDYRFILTLLHSMELKELNAIFSSLMLDHTDAQLHQLLYWAASIILFYFVSPILLVV
                     FILKERLTEYGFAMHFSWDSYKPYLIMLIIMVPVIAYFSGTQSFLANYPFYTVERAEQ
                     LYPNFVIWQFFYLLQFIALEFLFRGFLLHGTKAKFGFYAIFVMMIPYCMIHFGKPLAE
                     TIGAIVAGIVLGIMSLKTRSIWPGVMLHYGVAIMMDLLVLYRKGIIL"
BASE COUNT         2891 a         2198 c         1825 g         3070 t
ORIGIN      
        1 atgtgtaaag catgccgcca gcgttcaatc tgagccagga tcaaactctt cagtttaatc
       61 actcatatat ttacaatatt aattacttat attgctatca agcgcctaca atttattggt
      121 tattagattt taaagaactt atcgctttca aaattactta ctgcgcgatt gaagatgtgc
      181 attatacaag cattttcatt ttggtcaagc tttattgtaa aaaatgtaga gaatcgatgg
      241 atcagtaaaa ataatatggc tttagactaa tggatttagt ctaaagccat attattacta
      301 atatttaacc cgcactcgat aaacaaacgt cgcttcaccc tccgctgaaa ctggaatctt
      361 ccactcaaca gtgttagcag atattttttt atgttttata gattcagaaa caattaccca
      421 atcacctgga attggttcct gaacctgcac aacgactgac tctttcttag catttctaat
      481 cgtaatttga tatgtcgttt caaaaatact tgtttgatgt atcgaacctg caatctgttg
      541 aaaatcagtt tgtatcttat cagcagttat atcaaatgca cttcccagtt ttagtttaat
      601 ctgttcgttt ttcggagtat ggtcaatttg atcttcgcca ataaactgac tataaccttg
      661 cacatctttc ttataaacac gaacgatccc ctttggcaac ggaataccga gctgttcacc
      721 tttattatca aagctaatta atacacttac tttgagcttt tggccaagca cgccatattt
      781 tccggagtaa tagtaatctg caccttgaag gaaaaatatt ttattaactg gaatttgact
      841 agcagacatg agagccactt gcttagtttg attttctgcc agcgttgttg gccgctgaag
      901 tgtataaaga tggtactcaa aaagtgattc ttctttcatc tgggcagcat cagccatttc
      961 taccgacata gccgccattt ttctaccaat gcgttgttca gattgaattt gattaacatc
     1021 cccagcaacc aattgcaatc tagcctgagg atacgccacc ccgctttgat tggttagcgt
     1081 cactaatcca tttaaatcga gcttactatc attttcactg aggaccgcaa cataatcggc
     1141 acgccaagaa agaccacccg tcaagtacga gagctcaagt tcatgcttac ccgattctgg
     1201 gctatgcagc aatagtaata aagtaggttt atcacgcaaa ttttctggca cacctggaaa
     1261 taccattcgt ccaggcgcac ccgtttcaat acgattatta aatttgagaa caattccctc
     1321 attggttgag agaacgaccg ccgtttcatt tgtttctgtg ccagtagcca aattagtccg
     1381 gaccacagta atttcttttc ctacatgttt ctctaacaat ttttgtggcg tcagcaggtc
     1441 aaaatcaaaa ttttgctcaa aaacatgaaa accagattga gatgtttgat taccgagtaa
     1501 agcggtttca ggacgaatta gtgcggaaac ttcacgccag gccagctgat taatgttttg
     1561 acttaactca acattacgag aatccttaat taaggccaga ttttcgttat aaatcgtaac
     1621 tgtaatactt ttttgatcct ggctcgttgt gattttttcc gatgcctgta gtgatacatt
     1681 acccaaaagc atactcatac taaaaactat tgcaaagacc gtttcaattc gtaccttttc
     1741 ggtaatatca ggaaaatatt tttccccggt tatcttcctc caattcctca tcgcttgcag
     1801 agctgtcatt gtcattggtc gtgctctcag agtctaattc agtttgccat ccgaaatcta
     1861 tttcgtttat ttcaacatat tcaaccaact ctgtcgaatc ttcgattgga ctatctgcta
     1921 aatcctggat atctgcacga aattcctcat catactcatt ttcatcggaa agttctgatg
     1981 actcttcctc ttcatattgt tcaattaatg gagaaccaac ataatcctct tgagagaaag
     2041 tagcctgttc ttcagtcgtt tcttctgctg attggttatt acgaattatt tctgtttctg
     2101 caagatcttg ttgaatttgc tgtgaggctt gtgcaattgc acgttgatcc gctttcgtgc
     2161 gtgcatcggc tgcttctttt gccatggctt ctacggcttc tcttgcgata gcttgttcaa
     2221 tcgcaagcgc atcactttca attctagctt gaattgcatt aagcgctgaa gtctcagcct
     2281 gaactctagc aaacgttgtt gaagcagcta tttctgttgc tttcaatttt tcttctgttt
     2341 taaccgcagc aacaattctg gcctgcgcga tcttggatgc taattctgtc attctttgct
     2401 cattctcaat gcgagaagta atagaaattt ttgcttgcac gattgcatca tgccgtttac
     2461 gagcatccgc cgctaaccgt gcctctatct ctgccagttc aattgcagct tcagcagcaa
     2521 gcttatccac tttagctcta tcttctactt cagctgctgc tttcgcctct gcggccattc
     2581 tttcttccgc agcctgtttc gctgtcgctt ccgctatcgc tcgctcacgt gattttttga
     2641 ttgcctccgc ctgcgctttg atacgctcct tcgttgcttc aactgcagca ttatcagcat
     2701 tcattttggc actaagtgtt gctacctctt gctgttccgc ttgagcacgg ctctctgctg
     2761 aaaattgagc ttccttctcc gctgtaattt tttgattaat ttcttccaat agccgtgctt
     2821 cagcttgagc acgagaaatt gcaatagctt cagcttccaa ctgagctttt gcggtcgctt
     2881 ccgcatttgc tgcggcagcc ttatcagctt cagctcgtgc aattgctgcc tctgctgcct
     2941 ttgcatcagc ttctgcacgt aattctgctg cagttttagc atttaactca gcgtcgatct
     3001 ttgcctgagt tacttgctca gcttgaattt cagcatacag ttttgcttct tcagcctctc
     3061 tcgatttagt ttcagctgtc aaatgttctt gcgcttttgc aagtgctttt gcttcaactt
     3121 ctgcacgcaa gcgtacttca tccaatattt gacgttcagt ctctgtttta gcatgcgcca
     3181 atgctaataa cttttcttct tcttgaattt ttactctagc catcgcggct gctttagctt
     3241 ctgcttcagt tgcagaaagt gctaataatc tcgcttcttc ctcaattttt cttcttacga
     3301 ttgcggcttc gatcgctctc tcttcagctt cttcttttgc tttaatttga tcaaggatca
     3361 gtgcctcttc ttttgctcga acacgtgttt tctcagcaat aagtgtttca gcttgaactt
     3421 ttgctcggag cgtagcaaca atctctttct ccgctttgat tccatcttca atagtttgtc
     3481 gtaattttgc ttcaagctct ttacgtgctt gagcttcttt gctagcaagc gcttcagctt
     3541 ttgctctggc acgcgcttct tctgttgcag ctgtttctgc tttaattctg agctcagcag
     3601 ctaatcgtgc gctggcttct gcacgagctc tagcctcagc tgcgacagaa gctttagcat
     3661 cagcttcaat tcgttttttt gcttcctgaa aagcagtctc agcactcaac tggggaggga
     3721 tactattccc tgctgataat aattcataat tttctgaatc aacacaatgc ttataggtta
     3781 ggtctccatt aagcttttca ctcggattaa gcaaaacaac gtttgaatca gaatctgagt
     3841 caacatgagg agattgaata tttaggatag acttcatcaa acgagattta aatgtcatgg
     3901 cagctcaccg cacaaaaagt ggaatgttta tcaaatactt aggaagtaat gcacgattaa
     3961 attaaatact taagtattac agtatcaatt tctttagcaa aacaattgtt agaagaaagg
     4021 gggaaatact atctgtttac aaaattagct ttcctaaaac cacgcctaac gctattcagt
     4081 gggtgatggc tctatagttg acggcgttat tatttccagc atgatctgca atccattctg
     4141 gaacatctgc tcaaggatag gtaaataata aggcaacacc aacgacatca tcgctaaacc
     4201 aattgctaac gttagtggaa aaccaaccgc aaaaatattc aactgtggag cagctctcgt
     4261 cagaatgcct aatgccaagt ttgcaatgag tagtgtcgtt aagatcggga gtgataattt
     4321 catcccgagt aaaaatattt cacttcccca gtttgccaaa atgctgaatg atagcgaagg
     4381 catgcccgct gtccctattg gtaaaacgct aaaactttgt gcaagaatgg caatcatttg
     4441 caagtgacca tcaattgcaa gaaaaaccaa gagggcaatc aaccctaaaa atcgccctat
     4501 caattgaact tgacccgagt tctgtggatc gaaaaaaagg gcaaagccca gccccatctg
     4561 caagccgatt atttcacccg ccatctcgat cgcaacaaag ataactcgca tagcaaaacc
     4621 cattgctaag ccaataacaa tttgctgcat caagatcaag aaaccaattt cagaacctgg
     4681 atctaccgaa ggcaacgttt gtaaggttgg aacgatggtc agagtcaata ggagtgcaaa
     4741 acctattttg acttgtatcg gaatactgcg atgactgaga atgggggcac ttgagataaa
     4801 cgcaagaatt ctactaagtg gccagaaaaa actcgctaat aatgtgctga gctcggcata
     4861 agtgaagctg atcatgacct aaatcagtaa ttgtaatagc ttctacaact aacccgtcgt
     4921 cagccaaggg atattagcat acaatctttg cacgtaatcc atcataacac ttagcatcca
     4981 aggccctgct aataccatca ctgcaaacat aatcaaaagt ttggggatga acgatagcgt
     5041 catttcatta atttgcgtcg ctgcctgaaa aatgcttacg atcaaacctg ttaccaacgc
     5101 tgcgagtaat agcggcgcag aaaccataaa tgtaatttct agcgcgtgcc taccgatggt
     5161 aattgcgctt tctggactca ttgcataatt cctttaagtg taaaaacttt gtgtcagcga
     5221 accgattagt aaatgccaac catctaccaa tacaaacaac attaatttaa atggaagtga
     5281 aatgatcatg ggcgataaca tcatcatccc cattgccatc aacacgctgg ctacaaccat
     5341 atcaataatt agaaacggta taaaaacaat gaagccgatt tgaaaagcag ttttaagttc
     5401 actcgttata tacgctggga ccaaaatctt taatggaacc tgatcgcggc tttcaatttc
     5461 ctcgctattc gatatttgtg taaataatgc caaatctgct tctcttgttt ggcggagcat
     5521 aaaagctttt aggggtacac tcgctttttc accggcttca aatagattaa ttttatcttg
     5581 tgaaaaaggc acatacgctt cgctataaac tcgatcaatg acaggcgaca tcacaaacag
     5641 cgtaagaaat aacgttaacc cgattaaaac ttggttaggc ggtgaagatt gcgtaccaag
     5701 tgcaagccgt agcaaggata atacgatgat gatccgcgta aaactcgaca tcatcaggat
     5761 gagcgcaggt ataaatgtta aagtcgtgaa tagaagaagc gtctggatac ttaatgtata
     5821 ggcggttcca ccattctgag tcggtgtgct ggtaaatgct ggaaaatttg attgctgtgc
     5881 ataaacaggt aaaaccatca ggcaaagcgt gattaacaat aaaaacaata acgatcggtg
     5941 ccaggtaacg attgcttgtc ttttatcagt cattattttc acccattcac aatacgatga
     6001 ttattcatct tgttgctttt taatattttt ttgtagctgt gcaaaaaaat cattcgttcc
     6061 ctcagtactt aactgctcac tccctgtgct actagctatc ttctcaattt gatgtagaag
     6121 attaatatgc cctggcgcaa caccaagaac caaccaagca tttgcaattt caacgatgac
     6181 gatccgctct cgttgcccaa cagctgcagc ggccacaact ttaattaaat tggattgatt
     6241 accaggccct attccaaaac gtttaagtaa ccaagcgatt actccaatga cagccaatac
     6301 caacaatagg gccaagaaca tatgtagcac actgcttccc gttacgagcg atggcatagc
     6361 agctatctca ggcttagcaa cctcttccgc tcgaagtggt acttgcaaat tcattatgag
     6421 aaagagtagt aagaaatgaa cttttagcat tatcgattaa gcttccgaat tcgttcgctt
     6481 ggagtaatga tatcggttaa gcggatacca aatttttcat tgaccacgac gatctcaccc
     6541 tgagcaatta ggcaaccatt caccaaaaca tccattggct cacctgccat tccatctaac
     6601 tcaacgattg agccttgcgc tagttgtaat aaatttcgga ttgcaattct tgttcgccca
     6661 agctcaacag ttagttgaac tggtatatca agaataatat caatatcatt cttggcgtta
     6721 tttaacacat tgtcttttga aaactgttca aaaacgggtg ttgatgctgt tagcgtgtcg
     6781 ctagatattg attgatggtt actctcagga atcgctgcag ggtctgatgc tgtagatgac
     6841 tgctcttcca tagcttgtgc ccaatcatca gcttcaattg gcttttctgt ttgttctgtt
     6901 tcactcatag cttactccgt agaattttca gaatgggaaa gaatcgattt aactttcaat
     6961 gcatattgcc cattaagaac accatattga cattccaata atggcacacc atcagcaaga
     7021 gcagtaacag tgtcgggaat atccaatgca acaacatctc cttcttgcat atttagaatc
     7081 tgatttaggg taatcttcgc atgcccaata tttgcgatta gctccacttc agcactttgt
     7141 acctgcttag ttaatgacct gacccaccgt ttatcaagct ctaattgatc accctgtaaa
     7201 gtactactca gtgtgtcgcg aatgggttca agcatggtgt aaggaatgca agtatgaaat
     7261 tcacctcctg ttccaccgat gtcaaccgta aatgtggttg cgataacaat ttcacccggg
     7321 gtagcaatgc ttgcaaactg gggattcatt tctgtgcgta cttgttcaac atcaatggga
     7381 tatactgatt tccaagattt ttggtagtct tcgtaaacga cttcgagcaa tcgagaaata
     7441 atgcgttgtt ccgttagcgt aaactcccta ccctcaactc gccaatgaaa acgaccatct
     7501 ccaccgaata aattatcgat aatcaagaaa atgagcttgg gatcaaaaac aattagagcc
     7561 gtacctcgca gtggcttcat actaaccaaa ttcatattgg tcggtaccac taacgatctt
     7621 ataaattcac tatatttgac cgttttcatc gtacctggcg aaatatcagc cgcacggtgc
     7681 atgaaattgt aaagcccgat ctgtaagagt ctagcaaatc gtgcattaat aatttcgtaa
     7741 gttggcatgc gaccacgaac aatccgttct tgagtcgcca tattataagg acgaatacct
     7801 tctggagcgg tagacgactc tctttgagtc gcgcttgctt cttctggctt atcatcaccg
     7861 atacccgctt ggctcaataa tgcatcaact tcatcttgag aaagaaaatt ctcagccata
     7921 ggttaatatc aattactgaa taatgaatga gttaaacagc gtttctttga tatcttcatt
     7981 aaaatcttcg tcccccacaa tcaattgaat ttcttcaacg atgactttgc ccaattgctg
     8041 cttaccttca agcgtgaaaa tttctgaagc ttttttactt gaaagtacta gcaaggcttt
     8101 attccgaatt tctggcattt tttctttaat gatgcctaca acttcggttt gattgacttt
     8161 tagagatatt tctacttgaa gataatggcg ttcaccgtct tcttctggct gaagattaac
     8221 tgtgaaaggc tcaaatttaa taaactcggt tggttttttc ttctttttct tcttgggctc
     8281 ctcatcttct tcgccttgct ggctttgcat aaagtaccaa gtaccgccca cgccagcacc
     8341 aatcgataaa attgccacca ggataagcat aatcattttt ttcttgccac ctgtactctc
     8401 ttttgcaggg cttccggctt cagcactcgc gctcattttt cctcctttct atgcaaaaat
     8461 ttttataaat tatgtgtgaa tagatagaaa agcgatgtgc agaacagtac tctaatccat
     8521 ggttattttt aattctgctg ataaaatcaa gataaacacg ctaggtagga aggtgaatct
     8581 actgaaacaa aatagctttt aacaagaatc gaaataagat cgatgattga tgcgaggatt
     8641 atcgctagag tattatccct ttccggtata aaaccaataa atccatcata atcgcaactc
     8701 cataatgtag cattacacca ggccagatac ttctggtctt caagctcatg atacctagta
     8761 caataccggc cacaatggct ccgattgttt cagccaacgg ctttccaaag tggatcatac
     8821 agtaagggat catcatcaca aagatggcat agaacccaaa tttcgcttta gttccatgta
     8881 ataagaaacc tcgaaacaaa aattcgagcg caataaattg tagcaagtag aaaaactgcc
     8941 aaataacaaa atttggatat aactgttcag cacgttcaac cgtgtagaat ggataatttg
     9001 ctaagaagct ttgggtcccc gagaaatagg cgataacggg caccataatg atcaacataa
     9061 tcagataagg tttatagcta tcccagctaa aatgcattgc aaatccgtat tctgttagtc
     9121 gctcttttaa gataaacacg accaagagaa taggagaaac gaaataaaat aaaataatgg
     9181 aagcagccca ataaagtagc tgatgcaatt gagcgtcagt gtggtccaac atcagagagc
     9241 taaatatcgc attcagctcc tttaattcca tactgtgcag taaagttaag ataaaacgat
     9301 aatcaccaag atagtaatct agtgtcagaa tcaaagcaac ggcaatgcaa accaacgcaa
     9361 ccttaaaacc agactttttt ctcggttgcg gcacaagttt cagaatattt tgaaattgct
     9421 gagttaaatc aaatttggac accttaaatc tacaagtaag taactatttt aatagccgta
     9481 tagtttagct attttgattt aaccaagaaa gccctgtgaa aaagtaatcg cataaatttt
     9541 atttttgata acgtactagt ctttattgcc aaaactttga atctataaaa agtacggtac
     9601 acttcattca aggagcgtat ttacttagaa aagctactta tttaagtaaa taccagttca
     9661 tagagaaagg ctttaaaacg agtcagtata aaaaatttca gctggtacca tctgcgcacc
     9721 aaacatgggc aagttggtta gcccagcagg gtgcgtcgat gaatgtgcta caagaactcg
     9781 gcggctagga gtcagaagaa atggtcagac ggtatgctca tctatcaaaa ccacagcttt
     9841 tggaacatgc caaactcgtc tctaatgcct ttattggcac aaatctggca cacccaaaga
     9901 aaaaagaggc tagagaagat ctctaacctc ttaattttat ggtgggtctg gtaggacttg
     9961 aacctacgac caagggatta tgag
//
LOCUS       DAOQKQ010000096         9874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_3235_length_9874_cov_5.688257, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000096 DAOQKQ010000000
VERSION     DAOQKQ010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 9874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9874
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3235_length_9874_cov_5.688257"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..982
                     /locus_tag="PKZ49_07555"
                     /pseudo
     CDS             122..982
                     /locus_tag="PKZ49_07555"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
     gene            1047..1553
                     /locus_tag="PKZ49_07560"
     CDS             1047..1553
                     /locus_tag="PKZ49_07560"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HND36456.1"
                     /translation="MLPDGFLFGEGVKTTLKRELLEEFNLHTIVRLPKGVFSPYTSIA
                     TNLLFFEKSGSTQEVWFFEHPYPEGYKSYSRSKPLTIAEFEREKSWWGGSARKDRKTT
                     EYAWKVSAQDIAERNYNLDCKNPHEVEVNHRAPEELMAEYQQIVKQLEDAQQDLKAEL
                     MTCLGKKS"
     gene            1649..3517
                     /locus_tag="PKZ49_07565"
     CDS             1649..3517
                     /locus_tag="PKZ49_07565"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HND36457.1"
                     /translation="MTLAMQGKLVAQDPNDPPASELLKAIEAEKQRLHVTSSLRKQGS
                     SEGKNLDSLLRGNDGMKIKPLPPIKPKEVPYELPCGWEWVRLGNICSYIQRGKGPNYV
                     DFSMHRVVSQKCVRWYGLDIGPARYIDPISLEKYESFRFLRAGDLLWNSTGTGTIGRA
                     CIFPQELEKSKIVVDSHVTVVRSIKINSLFLWRWIQSPIVQNDIESSASGTTNQIELN
                     TTTVVNQLMPLPPLPEQHRIVAKIDPLMAKCDELEKLRKERQEKRLAVHAAAIKQLLD
                     NPPSFPRRRESSSTEELESHSPSTRGQACGDDEKELGSDGVSDSSWDFIQQHFGELYT
                     VKENVAELRKVILQLAVMGRLVPQGPNDPPASELLKVIEAEKQRLHATSSLRRQGSSE
                     GKNLDSRLRGNDGIKIKSLPLIKPEEVPYELPLGWEWVRLGNLTQVITKGSSPNWQGV
                     QYTDKDKGILFITSENVGSYELLLEKRKYVEEKFNLIEPRSILKKRDILMNIVGASIG
                     RTAMFDLDEVANINQAVAIIRLIKKLDHEYFLRFFNSQTCLSYMFDKQVDNARPNLSM
                     GNIALFAIPLPPLPEQHRIVAKIDQLMALCDKLEQQINAANHKQSELLNAVMASMS"
     gene            3800..4471
                     /locus_tag="PKZ49_07570"
     CDS             3800..4471
                     /locus_tag="PKZ49_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HND36458.1"
                     /translation="MNRSFTTVSQSSTSALATNKVLRNTYMLLSLTLLFSGLTAGLSM
                     FLNMPPMTYMISVIGGMVLAMFVLPRFAHSSAGIGIVFLITGLLGFGLGPMLTMYASL
                     PNGGNIITLSLGGTGVIFLGLSAYALATRKDFSFMGGFLVVGFLLVLIAAIANIFLQI
                     PAMSLAISSVVILIMSGFILYDTSRIIHGGEDNYVLATIGLYMTIFNIFISLLQILGI
                     MGKDD"
     gene            4565..4744
                     /locus_tag="PKZ49_07575"
     CDS             4565..4744
                     /locus_tag="PKZ49_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36459.1"
                     /translation="MDWMQIVSALALGLFVVFLFPTAINMMKNSPKGSAADWQSVIVP
                     MVIIILFIMFLIQLV"
     gene            4755..5273
                     /gene="msrB"
                     /locus_tag="PKZ49_07580"
     CDS             4755..5273
                     /gene="msrB"
                     /locus_tag="PKZ49_07580"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HND36460.1"
                     /translation="MKRRAVLKTLATASMFPFMAACWRIPEIGEAANPITPLKKSEKE
                     WRELLSQRAYQILFEEETERPESSNLVYEDREGTYLCAACYLPLFESQHKYESWTGWP
                     SFTQPIAGHVATQLDFKLIKPRVEYHCVRCGGHQGHVFQDGPPPRGERWCNNGIALKF
                     VSKDEPLPALRT"
     gene            complement(5283..5477)
                     /locus_tag="PKZ49_07585"
     CDS             complement(5283..5477)
                     /locus_tag="PKZ49_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HND36461.1"
                     /translation="MQFSITEVCNRVAQSLRLMVGVPEYSQYVAHMKTAHPDRTIMTE
                     VQFYRERQEARYGGKVSRCC"
     gene            complement(5494..7567)
                     /locus_tag="PKZ49_07590"
                     /pseudo
     CDS             complement(5494..7567)
                     /locus_tag="PKZ49_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380607.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
     gene            complement(7647..8429)
                     /locus_tag="PKZ49_07595"
     CDS             complement(7647..8429)
                     /locus_tag="PKZ49_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HND36462.1"
                     /translation="MTTYNTPDRPDFIRQQYAFAAHIRNPKQFPCPEGIEARRMAVYR
                     ELFYNNVEAFIANTYPVLHNIMTEDRWHAMVQDYFANHLAQTPLFPEMPREFLKYLEH
                     ERQPQPDDPPFLLELAHYEWVELALSLFDEKIDNNAINLTGDLLEGIPVISPLAWVLS
                     YRFPVHKISPTFQPQQADEANPTHLIVFRDAEFDIHFIETNAITVHLLQRITHNTNQS
                     GQTILQRIAVEMNHPQPDMIVTYGEQLMQQLKEQGILLGTVR"
     gene            complement(8398..9264)
                     /locus_tag="PKZ49_07600"
     CDS             complement(8398..9264)
                     /locus_tag="PKZ49_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HND36463.1"
                     /translation="MNSFKYPVHGVGLGLRRVMLSTLADQNTESVNFWEVAPENWINV
                     AGRHGKQFRELTEKHPVICHGLSLSIGSPAPLDETFLQRLKQFLKEHRIRYYSEHLSY
                     CSDDGHLYDLMPIPFTEEAVRYVAERIRHVQDMLDQRIAMENISYYAAPGKQMEEIDF
                     LNAVLEEADCDLLLDVNNIYVNSINHRYDAEAFLRQLPAERIRYIHVAGHYQEAEDLL
                     VDTHGSSVIDPVWQLLEKAYQHFGVLPTLLERDFNIPALSELLEEAKIINHLQAKWLN
                     CACDHIQHARPS"
     gene            complement(9277..9621)
                     /locus_tag="PKZ49_07605"
     CDS             complement(9277..9621)
                     /locus_tag="PKZ49_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36464.1"
                     /translation="MSKKIIKPVSIALGTALLTTLSASNFAAESNPFAMKTLSSGYMQ
                     LASADDAKKGEQDSQPSTDQKKADHEGKCGEGKCGAKGEMKKNMEGQCGGKKAEQEGK
                     CGEGKCGSSNKK"
BASE COUNT         2816 a         2389 c         2211 g         2458 t
ORIGIN      
        1 gctaatgtgg cttgactcaa actaaaaggt ctccggaaaa cccggagcga ttcaagatag
       61 aagaactgga gataacacaa ccatgtccct caataccctg gttaaatcta ttcaggacat
      121 catgcgcaaa gacgttggtg tcgacggcga tgcacagcgc atcagccaga tggtttggct
      181 gatctttctg aaaatcttcg acgacaagga acaagaatgg cagctcacca ttcccggcta
      241 caaatcgccg ctgcccagcc gcttccgctg gagcaactgg gcaagggatg cggagggcat
      301 gaccggcgat gagttgatcg atttcgtcaa caacgatctt tttccggcac tgaaaaaact
      361 ggcgacggct gccggtgtgt cgccgcacgg taaagtggtc ggctcggtgt tcgaggatgc
      421 ttacaactac atgaaatccg gcacattgct gcgccaggtg atcaacacca tcgaggcgga
      481 tgtcgatttc aacaaatcca gtgaccgcca cctgttcaac gacatttatg aaaaaattct
      541 cgccgacctg caatcggccg gcaatgccgg tgaatactac acccctcgcg ccgttactca
      601 gttcatggtc gacatgctcg acccgcagct cgggcaaatc attctcgacc cggcctgcgg
      661 caccggcggt ttcctcacct gcaccatcga acacctgaag cagcagatca aaaaccccgg
      721 cgaccgcacg cggttacagg aaagcatttc cggggtggaa aagaaaccgc tgccgcacat
      781 gctggccatg acaaacatga tgctgcacgg catcgacgtg cccaccaaca ttcgccacga
      841 caacacactg agccgtccgc tcagagatta cggcccgcgc gaccgggtcg acctgatcgt
      901 caccaacccg ccgtttggcg gcatggaaga agacggcatc gaaaacaact tcccgcgcaa
      961 ataccagact cgcgaaaccg ccgacttgtt catggcattg atcatgcacc tattgaaaca
     1021 cgacggcggc aaagccgcgg tggtgcgtgc taccagatgg ttttctgttc ggcgaaggtg
     1081 tcaagaccac gctcaaacgc gaattgctgg aagaattcaa cctgcacacc atcgtgcgtt
     1141 tgcccaaagg cgtattcagt ccatacacca gcatcgccac caatctgttg ttcttcgaga
     1201 aaagcggatc gacccaggag gtctggtttt tcgagcaccc ctatcccgag gggtataaat
     1261 cctattcccg ctccaaaccg ctgaccattg ccgaattcga gcgggaaaaa agctggtggg
     1321 gcggcagcgc acgcaaagac cgaaaaacca ccgaatacgc ctggaaagtc agcgcgcaag
     1381 acattgccga acgcaattac aacctcgatt gcaagaatcc gcatgaagtg gaagtcaatc
     1441 accgcgcacc ggaagaactg atggcggaat accaacaaat cgtcaaacaa ctcgaagacg
     1501 cgcagcagga cttgaaagcc gaactgatga cctgcctggg gaaaaaatca tgatgacctc
     1561 acgccttggc gtgaacgagc ttctggaaca acacttcgac accgcctttg ccgccccaga
     1621 cggcatcaaa aaactttgag aactgatctt gacgctggcg atgcaaggta aattagtggc
     1681 gcaagatccg aacgatccac cggccagcga attgctgaaa gccattgaag ccgaaaaaca
     1741 gcgactccac gtcacgtcat ccctgcgcaa gcagggatcc agtgaaggaa aaaacctgga
     1801 ttccctccta cgcgggaatg acggcatgaa aatcaagccg ctgcccccga ttaagcctaa
     1861 ggaggtgcct tatgaattgc cgtgtgggtg ggagtgggtg aggttgggta atatttgttc
     1921 atacatccag cgaggaaaag gacctaatta tgtcgatttt tcgatgcacc gcgtggtttc
     1981 acaaaaatgt gtcagatggt atggtcttga tatcggacct gcaagatata ttgatccaat
     2041 ctcactggaa aagtatgaat cgtttcgatt tttaagagct ggagatcttt tgtggaattc
     2101 gactggtaca ggaaccattg gtagagcgtg catctttcca caagaactcg aaaaatctaa
     2161 aatcgtagta gattcccatg taacagtcgt tcgctcaatc aaaattaact cactgtttct
     2221 gtggcgttgg attcaaagtc ctatcgttca gaatgatatt gaaagttcag catcaggcac
     2281 aacaaatcag attgagttga atactacaac tgttgtcaat caactaatgc ctctcccgcc
     2341 cctccccgaa caacaccgca tcgtcgccaa aattgacccg ttgatggcga aatgcgatga
     2401 actggaaaag ctacgcaagg aacggcagga gaagcggttg gctgtccacg ctgccgcgat
     2461 caagcaactg ctcgacaacc ccccgtcatt cccgcgcagg cgggaatcca gtagcacgga
     2521 agaattggaa tcccactccc cgtctacacg aggacaagca tgcggggatg acgaaaaaga
     2581 gctcgggagt gatggtgttt cggatagcag ttgggacttt atccaacaac atttcggcga
     2641 actctacacc gtcaaggaaa acgtcgccga actgcgcaaa gtcatccttc agctcgccgt
     2701 catgggccgc ctcgtccccc agggccccaa cgacccgccc gccagtgaac tgttgaaagt
     2761 catcgaagcc gagaaacagc gacttcacgc cacgtcatcc ctgcgcaggc agggatccag
     2821 tgaaggaaaa aacttggatt cccgccttcg cgggaatgac ggcataaaga tcaagtcact
     2881 gccgctcatc aaaccggagg aagtgcctta tgaactaccg ctagggtggg agtgggtgag
     2941 gttgggtaat ctgactcaag taataactaa aggctcctcg ccgaattggc agggggtgca
     3001 atatactgat aaagacaaag gcattctttt catcactagc gagaatgtgg gttcatatga
     3061 attactacta gaaaaaagga agtatgtgga agaaaaattc aacctgatag aacctagatc
     3121 aatcctcaaa aaaagagaca ttttgatgaa tattgttggc gcatctatcg ggcgaactgc
     3181 tatgttcgat cttgatgaag tggcgaatat taatcaggca gtagcaatta ttcgtttaat
     3241 taaaaaattg gatcatgaat atttcttgag gtttttcaac agccaaacct gcttgagcta
     3301 tatgtttgat aagcaggttg ataatgcgcg ccccaacctt agtatgggaa acatcgcatt
     3361 atttgcaatt cctctcccac ccctccccga acagcaccgc atcgtcgcca aaatcgacca
     3421 gttaatggcc ttgtgcgaca agctagaaca gcagatcaac gctgccaacc ataaacaaag
     3481 cgaattgctc aatgcggtca tggcaagcat gtcatgataa cgtttagcac attgcattct
     3541 gagaaaaatt aaaacaaaag tcgatgaatt tgttatctta ttcatcgtaa tagcactttt
     3601 atctgagctc aaaaatcatt taaactgggc tcttgggtcg gtatttttct aaatcatcga
     3661 aaataattga tctataatta aaaaagtagg tctaacctac gttaacaaga gatgagcgca
     3721 gaagacggta tttaatcgat atcgtgtgct taaatacaat gataatattc acatttatgt
     3781 ttttgttgga ggtcacaaaa tgaatcgaag ctttacaacc gtttcacaaa gttcgacgtc
     3841 tgcattagct actaacaagg tattacggaa tacctacatg cttctatcgt tgacattatt
     3901 gttcagcgga ttgactgctg gactctcaat gtttctgaat atgccgccaa tgacctatat
     3961 gatttctgtg atcggcggca tggtgcttgc aatgtttgtg ttgccgcgtt ttgctcattc
     4021 tagcgcgggc attggtattg tttttctgat taccggtttg ctggggtttg ggttaggtcc
     4081 catgttgaca atgtatgcct cactgccaaa tgggggcaat atcattacct tgtcgctagg
     4141 tggaaccggt gtgattttcc tgggattgtc agcctatgcg ctagctaccc ggaaagattt
     4201 tagttttatg ggtggattct tggtcgtggg ctttttgcta gtattaattg ctgcgattgc
     4261 aaatattttc ttgcaaatcc cagcgatgtc actcgcgatc tcatctgtgg ttattctgat
     4321 catgagtggt tttattcttt acgacaccag ccgcattatt catggcggtg aagacaacta
     4381 tgtgttggca acaatcggtc tttacatgac gattttcaat atcttcatta gcttgttgca
     4441 aattttaggc atcatgggta aagacgactg attcttgtcg ataagttacc tatgttagac
     4501 aaacaaaccc cggttcatcc ggggtttgtt tttttgtgct caataataaa ggaaaaacct
     4561 gagtatggat tggatgcaaa ttgtctctgc attagcgtta gggttgtttg tagttttttt
     4621 atttccaact gctatcaata tgatgaagaa cagccctaaa ggcagcgctg cagattggca
     4681 atcagttatt gtgccgatgg tcatcattat tttatttatt atgtttttaa tccagctagt
     4741 ttgagtgcat tagaatgaaa cggcgcgctg tattaaaaac actagccact gcgagtatgt
     4801 ttccgttcat ggctgcttgc tggcgaattc ctgaaattgg cgaagctgcc aatcccatca
     4861 cgccactcaa gaaatctgag aaagaatggc gtgaattact ctcacagcgc gcttatcaaa
     4921 ttctcttcga ggaagaaacc gaacgacccg aatccagcaa tctggtttat gaagaccgtg
     4981 aagggactta tctttgcgca gcgtgctatt taccactatt tgaaagtcag cacaaatatg
     5041 aaagttggac aggctggccg agtttcactc agccgattgc aggtcatgtg gcaacccaac
     5101 tcgatttcaa gctgattaag ccccgtgttg aatatcactg cgttcgatgc ggcgggcatc
     5161 aaggtcatgt attccaggat ggccctccgc cacgcggtga acgttggtgc aataatggga
     5221 ttgcgctcaa atttgtatct aaagacgaac ccttgccagc gctaagaact tgataccaac
     5281 cgctagcaac agcggctaac tttccctcca taacgcgctt cttgccgttc acgataaaat
     5341 tgcacctcgg tcataatcgt tcgatctgga tgcgctgttt tcatatgggc tacgtattgg
     5401 ctatattcgg gtacacccac catcaagcgc agactttgag caacgcgatt acaaacttct
     5461 gtgatgctaa attgcattgt gttactcctt taattatttt ccggtcggaa cggtaaccgg
     5521 taacagctca aaaggcgcct cctgtaccgt tggatgttga ttagtacgcg cttgcagcac
     5581 ggttcggaga ccaaaccaca acatactgat caggactgcc ataaacaacc ccgctaacgt
     5641 tgcattgaca taatcgttaa taatgacttg ctgcatttgg tcgatcgatt tggcaggcgc
     5701 gagtaccaat ccttgcatta cagcctcctg gtatttttcc gcatgggcca gaaaaccaat
     5761 gcgcggattg tcatgaaaga ttttctgcca cgctgcggtt aacgtgcata cacaaaccca
     5821 ggtaactggg atcatagtca cccaggcata gcgatcctgt ttcatcttaa acagtacgca
     5881 ggtacataga atcaatgcga taccagctag catttgattg gaaataccaa ataatggcca
     5941 caaggtatta atgccaccga ggggatcgac aacaccttga tatagaaaat atccccaacc
     6001 tgacacacaa ataaaagtag ccaccaaact ggctggaacc gattcggttt ttttcatggc
     6061 aggaataaaa gagccgagta aatcttgcaa cataaaacgc cctgctctgg tccctgcatc
     6121 gaccgccgtc aaaataaata aggcttcaaa tagaatcgca aagtgatacc agaatgccat
     6181 catggcggta ccgccaacta cctcagacaa aatttgcgcc atacccaccg ccaacgtagg
     6241 tgcaccgcca gtgcgcgaaa taatgctgtg ttctccaaca ttctgcgcgg tttggttaat
     6301 catttccggt gttacataaa acccccattg tgcaatggtc tgcgcggctg attcggcagt
     6361 gctaccaatg atcgcagcgg ggctattcat tgcaaaatat attcctggct caagcgtcgc
     6421 ggcagcgacc aatgccataa tggccacgaa ggactccatt agcattgccc cataaccaat
     6481 aaaacgcgca tcggtttcat tctgaatcat tttaggcgtc gtgccggatg caatgagcga
     6541 atgaaatcct gaaattgcgc cacatgccac ggttacaaac aaaaagggaa ataagtcgcc
     6601 agaccagacg ggaccagaac catctacaaa ttgggtcaat gctggcattt taagatctgg
     6661 cgcaatgatg agcactccta tcgcaagccc aaccgatcgt accgattttc aggaatgtcg
     6721 agagataatc acgtggcgct aataacagcc aaacgggtaa taccgaagca acaaaaccat
     6781 agccaatcag cgcccaagtc aattgctccc cggttagggt aaaaatcgca gcccatgcag
     6841 ggtcttcctg tacatattgc ccaacgacaa tcgatagcat cagcaataca aaaccgatga
     6901 gcgaaacttc accaatcgca caaggccgga aaaaacgcga ataaattccc ataaacatcg
     6961 caataggaat cgttgctgcc acggtaaacg tcccccatgg tgattctgca agcgccttga
     7021 ccacaatcag cgacaaaacc gctaacaaaa tcagcatgat aaaaaacgca ccaaacaacg
     7081 cgatcatccc tgggatttgc cctagctctg acttgatcaa atcaccgagt gaacgcgcat
     7141 tgcgtctcgt cgatacgaac aacacgataa aatcctgcac tgcacccgct agcacaacac
     7201 ctgccagcaa ccacagcatc ccaggcaaat accccatttg cgcggctaga atcggaccca
     7261 cgagcggacc tgcacccgca atcgccgcga agtgatgacc aaataacacg aatttgttcg
     7321 tcggaacatg atccaatcca tcgttatgct tatgcgccgg cgtcattcgc gttgcatcaa
     7381 gcttcaacac gtgattggca ataaataagc tatagaatcg atacgcaatc aaatagacac
     7441 agactgctgc aatgacgatc cacaacgcgc caatcgattc acctcgattg agtgcgatga
     7501 ctgcgagagc agaagcaccg aggagagaaa agagtaacca acctagttta ttcactatcg
     7561 aattcatatc taattagtat caaaaaatta ttatcaaatt gactacttga atggagcgat
     7621 gctagcgaat ttagtctaca ggttgcttaa cgtacggtac cgagcaagat tccttgttct
     7681 ttgagctgct gcatcaattg ctccccgtag gttacgatca tatcaggttg aggatgattc
     7741 atctcaactg cgatgcgctg taaaatcgtt tgccccgatt gattggtatt atgggtaatg
     7801 cgttggagca aatgcacggt aatggcgttt gtttcaatga agtgaatgtc aaattcggca
     7861 tcacgaaata cgatcagatg agttggattc gcttcatcag cttgttgagg ttgaaaagtc
     7921 ggtgaaattt tgtggacagg aaaacgataa ctcagcaccc aggccaaagg tgaaataaca
     7981 ggtatgcctt cgagcaaatc accagttagg ttaattgcgt tgttatcgat cttttcatca
     8041 aataaggata gcgctagctc tacccattcg taatgcgcaa gctctaacag aaaaggagga
     8101 tcatcaggct gaggttggcg ctcatgttcg agatatttca gaaactccct tggcatttcc
     8161 ggaaacaagg gggtttgcgc taaatggttt gcaaaataat cctgaaccat cgcatgccag
     8221 cgatcttcag tcataatgtt atgcagcacc gggtaagtat tggcgatgaa cgcttctaca
     8281 ttgttgtaga acaattcacg atacacggcc atacgccgcg cttcgattcc ttcagggcag
     8341 ggaaattgct tagggttacg aatatgtgca gcaaaagcat attgctgccg aataaagtca
     8401 ggacggtcgg gcgtgttgta tgtggtcaca agcgcaattt agccacttgg cctgcaaatg
     8461 attgataatt ttagcctctt ctaataattc agaaagtgcc ggaatattaa aatcgcgttc
     8521 caacaatgtc ggtaatacac caaagtgctg atacgctttt tccagtaatt gccagaccgg
     8581 atcaatgacc gaagatccat gggtatcgac caataaatct tccgcttcct gataatgccc
     8641 tgcaacgtga atataacgaa tgcgttctgc gggaagctgt cgcaaaaacg cttctgcatc
     8701 gtagcgatga ttgatactgt tgacgtaaat attattgaca tccagtaaca aatcgcaatc
     8761 tgcttcttct aagactgcat tcaggaaatc gatttcttcc atttgttttc ccggcgctgc
     8821 gtagtaggaa atattttcca tagcgattcg ttgatccagc atatcttgaa catgccgaat
     8881 ccgctctgcc acatagcgca ccgcttcttc cgtaaaagga atgggcatca aatcgtacaa
     8941 atgcccatca tcactacaat agctgaggtg ctcgctgtaa taacgtatgc gatgctcttt
     9001 caaaaattgc ttcaaacgct gtaaaaatgt ttcgtcgagt ggtgctggac tcccgatgga
     9061 aagcgataag ccatggcaaa taacaggatg cttctcggta agttctcgaa attgcttacc
     9121 gtgtcgtcca gcaacattaa tccaattttc aggcgcgact tcccaaaaat taactgattc
     9181 ggtgttttgg tctgcaagcg tgctcaacat cacacgccgt agaccaagcc caacaccatg
     9241 aacaggatat ttaaaactgt tcatggtaaa accttattat ttcttgttgg agctaccaca
     9301 cttaccttca ccgcattttc cttcttgctc ggctttctta ccgccacact gtccttccat
     9361 atttttcttc atctcacctt tagcaccaca cttgccttcc ccacatttac cttcatgatc
     9421 agcctttttt tgatctgtag atggctgact atcttgttca cctttcttgg catcatcagc
     9481 agaagcgagc tgcatatagc cactcgataa ggttttcatt gcaaaaggat tactctcagc
     9541 cgcaaaattg ctggctgata atgtggtcaa tagcgctgta cctaaagcaa ttgagacagg
     9601 tttgatgatt ttctttgaca ttaaactaat tctccataaa aagttaatct acgatgatta
     9661 caaattggac aagcagttaa tatagcagat ttctttgaag ttataaccct aacggtaaag
     9721 ataggaaaat cggataagca ggagaaaaaa tcgctggtag cgttccgaaa aataagaaag
     9781 cgaatatgaa acattccata ttcgcctttt cgaaacaact ttatttttta aagtagcgca
     9841 ctaatgagtg ccctacttta ttgatctgat aaac
//
LOCUS       DAOQKQ010000097         9780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_3284_length_9780_cov_6.115270, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000097 DAOQKQ010000000
VERSION     DAOQKQ010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 9780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9780
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3284_length_9780_cov_6.115270"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PKZ49_07610"
     CDS             <1..327
                     /locus_tag="PKZ49_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HND36465.1"
                     /translation="NGGNTEITTNEIVALAAQGDRLAEATMQCFFDRFGRAMATVVNI
                     LDPDVIVLGGGLSNIDRLYTEGRAKVAQYVFNDQFITPMLRNIHGDSSGVLGAAQLWR
                     HDDAFE"
     gene            517..1482
                     /locus_tag="PKZ49_07615"
     CDS             517..1482
                     /locus_tag="PKZ49_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36466.1"
                     /translation="MDRLTIIILMMLVSNIAIANYFNTHRSQKIIGSFVTEKSQIQEV
                     NSFSNAFAFAPASPRDITQYLDENKGRMLPVISISRLLLDEQTGMYHHDVTSIVEAIA
                     KAKHKDTEILFLMDEPFWWVRRACEEKGKAEACLDVANRYVETLSTFRIAGQLLRKHL
                     PGSGIIHIEAWAELVLQKKAYPEENVVMLDDAEYLGFDCYGGIEFCGSDEYGYYSQFE
                     YGTWVWDAMLAMESQQAIGRKLFVIPGTFLADGHFADIDTLINQIGFYGWILNQSDKI
                     GGFGAFLWGDMVENNKPFTGGRNILQATNFLKVIARYYGVKTNEK"
     gene            1811..4645
                     /locus_tag="PKZ49_07620"
     CDS             1811..4645
                     /locus_tag="PKZ49_07620"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HND36467.1"
                     /translation="MATNTKTNVLSTASFTKNSKANNDSFENLSSNNTTYVLDVLAND
                     SGGQSQTLWSIDDSASSSAPPSPDLLSPDEIGAINLSEKGANIWITEEGKVAYELTPD
                     LASALKALPQGENLVDTFSYAMRQGKGNNPLSWATATVTFAGVNDAPELTGAPTILSD
                     GIVNQPYSILATDLLSGYTDADGDPLSISNLTATHGLLTTIPDGWLFTPDSDYSGPVE
                     LNYDVIDNQGGSLPAQQNFTVITPGDNTAPSFLSSRPTDDQTTFGNDENIFLYFDEAI
                     KAGTGVITISNGLDTRVIDATDTSQISFTSSKGSHYVTINPHDDLIPNTTYNVQMGAG
                     AITDLVGNPFAGIADENTLNFTTVLSLPQLIGSNPWDDATQFKIDSNIELYFDETVMA
                     GSGDIIISNGVDTRIIPINDTNQVSFSKDGSTITINPTADLIPNTDYNVQIASGVITN
                     LAGNPYLGINDSRTLNFTTIPSNPLLIGSYPWDDSSEFQVDSDIVLSFDEGVKTGIGN
                     ITITNEADSTDSRVISVNDATQVLFDDSGYVIINPMDNLASNAEYSIQIDSGAIIDLA
                     GNAYAGISDNTSLNFTTIPTNPLLNWSNPPDDFNHFQVENNIELFFNESIIAGSGNII
                     ISNGVDTRIIPITDTSQVSFDQYYSAIVTINPTDDLIPNTTYHIEIESGVINDLAGDP
                     FLGISDDTTLNFTTTLNPFLIGSSPWDDSTDFKIDDNLWLSFDETVKAGVGNITITNE
                     TNPADSRMISVDDITQVSFDDFGNVIINPTADLIPNTLYSIQIDNTAITDLAGHAYIG
                     ISDNTTLNFTTIPSNPRLSFSTPADDTILFPTDGNITLSFDEQITAGIGNIIITNESD
                     TSDTHTFAINDTSQVTISGSKVTIDPSTDLMPNTTYNIQIAEGVIKDLAGNTYKGIQD
                     ADTLDFTTGDAFQTSTVGINEFNLPILPLG"
     gene            4723..6012
                     /locus_tag="PKZ49_07625"
     CDS             4723..6012
                     /locus_tag="PKZ49_07625"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HND36468.1"
                     /translation="MTTSQSQISNLLQKAAHCHQQGNFQDAEACCLKVCALVPNQPDT
                     LHLLAIIYAQTKQYELANNYFIKAITVAPQRADFLGNYANALWEQGNIDDALINCEKS
                     LALNSNQADVHNILGNVYLAQNRIEAAIGCFRNALQYRPNFPQALNNLGNALQKINKM
                     EEAVKSYSNALGLNENYPEAYNNLGQALKTLGRIEEARNYFLKAIALRPNFTQAILNH
                     QEVAMIWLAPLEGNKLYLRRYCKEDASYLRQCYQNTQFMGQYNHYIPRHQRAEELALK
                     LDEANKMHPCQLKSIDWVIMKKGANQPIGIANLVGIEFAHRRAEFLIGLPDTNNHAKG
                     LGLEATLLVLDFAFNKVNLNKITSIVYEDNTVSQQNALALGFIQESMLRDHIFDSTSG
                     KFLNLWGIGMTMNDFRNNLRIAKLSDRLLGKNITLVP"
     gene            complement(6070..6762)
                     /locus_tag="PKZ49_07630"
     CDS             complement(6070..6762)
                     /locus_tag="PKZ49_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HND36469.1"
                     /translation="MILLASSYEEMLDEWRRSLTDVSSVLCINDHELLNHELARRKPP
                     VLLIDHDFLGNDGSKKIEGLLALSPKTQIIISSPGISDEMEWKLFKSGIRGCYRDSIE
                     PGEIKRVVKAVWQGELWIRRKLVHHMLDELVVTTKEKKHIERAVNNLLENLTKREYEI
                     AILVGRGESNKQIARRLDITERTVKAHLTEIFRKLAITDRIKLALLVKDTAVSSNQTV
                     LHHSNNFPIASA"
     gene            6947..7579
                     /locus_tag="PKZ49_07635"
     CDS             6947..7579
                     /locus_tag="PKZ49_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36470.1"
                     /translation="MTRLFRSFPLVLKISSTLQMLLGLVLLIIGLSISHAENLLKSET
                     IIAHFEEKQSSTRNFNENITVTIFADGYVKVYRPSYYKKSGIFSGHLDPVALNQLWNQ
                     LTSPSLLNFNETEIQHRLKTAKNQQEKNTLLLQRVSDAPTIIIEIYPNRYMPTASIDQ
                     NNWNTTKRITWYALQWTVENFPELEELHELMEVRELFENILESSELKSLN"
     gene            7611..8972
                     /locus_tag="PKZ49_07640"
     CDS             7611..8972
                     /locus_tag="PKZ49_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36471.1"
                     /translation="MKDISTIILLIFTYGLMLNTVAGTFLFAEDNETADVITHPQGYQ
                     GQGTNLIVTVGISPSSLHGTEIALPIQNAINTWNQLIPTLGNIVSDERIPFNHFDFES
                     VVLHELGHCLGLAHPNLSSESGLSGENRNYTQTTRGSNNRYDLNSGSDGIAGSNDDLR
                     GDDANLHWFRRKNNNPFTIAEKIDITTYSRNLSDLPVGHTFAANGDRTVANLLGYQNT
                     EAVMQQGIIAGETQRTLTADDVATLKLAMSGLDRFAGTPDDYTLALTFIGFTDDADIV
                     VSFNNSRSSFAVCETRAEQLNSKHFAITRSRIYFNTNVQWYFNDLTSPNPPKLSYPSP
                     TIAANNQTGTLQLSRNDTLLLTVSLDPGVLLENPAEYWIRAITPTETFWLNDRFEFIP
                     SSQPIRAHVGEFNTINQFIIMKSSLKALQPGNYSITFAVDDNQDGIANGTFHNTVNIT
                     ITP"
     gene            complement(9024..>9780)
                     /locus_tag="PKZ49_07645"
     CDS             complement(9024..>9780)
                     /locus_tag="PKZ49_07645"
                     /inference="COORDINATES: protein motif:HMM:NF039822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HND36472.1"
                     /translation="DFEVPIIVLGATEIESNGYHIPLSELEVGDVVKINGFFGEDGIV
                     AESIYVLDGGLGKFRLRGLIKGVEPVEDGIFMQVLGIDILVDESTSIRSRGLGVVNNL
                     PISALQVGESVDVKGRYLDDTFFADRVEVGKRPDEHVRVKGKIIDLTNDIIEIVTIHE
                     AHLIVVRTGTTKIYGSLAEGEFVEVEGYLNQKLDVIAEKIIVDDDRNGYSRDYHHYES
                     KRSDDDRFGVINRNYGKHHGKKNGRDKDEDRDD"
BASE COUNT         3042 a         2068 c         1831 g         2839 t
ORIGIN      
        1 aatggaggta ataccgaaat tacaacaaat gagattgtgg cacttgcagc acaaggtgat
       61 agactggcag aagctaccat gcaatgtttt tttgatcgat ttggccgtgc catggcaaca
      121 gtcgtcaata tacttgaccc tgatgtgatc gtgttgggtg gcggattatc taatatcgat
      181 cgattgtata ccgagggtcg agctaaggta gcgcagtatg tgtttaatga tcagtttata
      241 acacccatgt tgcgcaatat tcatggcgac agctccggtg ttcttggggc tgcacagttg
      301 tggcgtcacg atgatgcttt cgaatagtta caatttgggt aaatataaat aatcgagaaa
      361 atcatttcaa ttttatattt gcaatacttc gaatagttag cagataaagt gtgatttata
      421 tctggtcctt gagtgagttt atgcaccgac gttaacttgc cataaatcta ccatcgtctt
      481 catcaatgaa taagtttctg atgaaggaga acgacgatgg acaggctaac gataatcata
      541 ctgatgatgc tagtctcgaa tattgcgatt gctaattact ttaatacaca tcggtcacaa
      601 aaaatcattg gatcgtttgt gactgaaaaa tcgcaaattc aagaggtcaa ttcgtttagt
      661 aatgcctttg catttgctcc ggcatctccg agagacataa cgcagtatct agatgaaaat
      721 aaaggcagaa tgcttccagt cattagcatt agtcggttat tgctcgatga gcaaacgggc
      781 atgtaccatc acgatgttac gagcattgtc gaagcaattg ctaaagctaa acacaaagat
      841 acagaaattc tatttttaat ggatgagccg ttttggtggg tccgacgtgc ctgtgaagaa
      901 aagggaaaag cagaagcctg tctcgatgtt gctaatcgat atgtagaaac cttatcaact
      961 tttcgaattg ctgggcaatt actgcgcaaa catttaccag gttcagggat tatacatatc
     1021 gaagcctggg ctgaactcgt actacaaaag aaagcctatc ctgaagaaaa tgtggttatg
     1081 ttagatgatg cagaatacct gggatttgat tgttatggag gtatcgaatt ctgtggctct
     1141 gatgagtatg gttattactc acagtttgaa tacgggacat gggtgtggga tgccatgcta
     1201 gcgatggaat ctcaacaagc tatcggacga aaattgttcg tgataccagg aacattcctg
     1261 gcagatggac attttgcaga tatcgatacg ctgatcaacc aaataggatt ttatggttgg
     1321 attcttaacc agtcagacaa gatcgggggg tttggcgcgt tcctttgggg cgacatggtt
     1381 gaaaacaata aaccttttac cgggggtagg aatatattgc aagcaaccaa cttcctgaaa
     1441 gttattgcga gatactatgg tgtgaaaaca aatgagaaat gaagcattct aagtttttta
     1501 tcgaattaat gtaaacaaat attcacggga acgagcagga aggccacctt tacctactcg
     1561 tggtagctta ctgtgattat cagctaaatt acacgctcat tcgcggaaaa atcgcaagca
     1621 gctcaacctt tcccacaaat tagctcagaa tttttctgaa atatgaaatt tacattattt
     1681 tgtaagatct ctgatattct atgtacatag atttttcatt gtggaaacat ctgctgattc
     1741 aggtatggat agccccactt cgataaattt atatcaaact cgatcatttt ctattttaga
     1801 ggataggttt atggcgacta atacaaagac caatgtttta tcaacagcta gctttacgaa
     1861 gaactccaaa gccaataacg atagttttga aaatctttct agcaataaca ctacctatgt
     1921 attagacgtc ctggcaaatg attccggtgg ccaatctcaa acactctggt caattgatga
     1981 tagcgcgagc agctctgccc ccccttctcc tgatctcttg tcacccgacg aaatcggtgc
     2041 tattaaccta agcgaaaaag gggcaaatat ctggataacc gaagagggta aagtggccta
     2101 tgagctaaca cccgatttag cgtctgccct aaaggcctta cctcaaggag aaaatttagt
     2161 tgacaccttc agctatgcaa tgcgtcaagg caaaggcaac aatcccctga gttgggccac
     2221 tgctacggtg acgtttgcgg gcgttaatga tgcccccgag cttacaggcg caccaaccat
     2281 attgagtgat ggaatcgtca atcaacctta cagtatcttg gcaaccgatt tgttatcagg
     2341 gtatacggat gccgatggcg atccattgtc tatttcaaac ttaactgcta cccatggatt
     2401 attgactaca atccctgatg gatggttatt tacgcctgat tctgattata gtggtccagt
     2461 cgaattaaac tacgacgtta tcgataacca aggtgggtct ttacccgccc aacaaaattt
     2521 tacggtaatt actccgggcg ataatacagc accttcattt ttgtctagca gacctaccga
     2581 tgatcaaacg acatttggca atgatgaaaa tattttcttg tattttgacg aagcaatcaa
     2641 agctggaacg ggcgttatta ccattagcaa tgggttagat acgcgtgtca tcgatgcaac
     2701 tgacacaagc caaatcagtt tcaccagcag caagggaagt cattatgtaa cgatcaatcc
     2761 acacgatgat ctcattccta ataccactta caatgtccaa atgggtgctg gggccattac
     2821 cgatctagtt ggaaaccctt ttgcagggat tgctgatgaa aatactttaa actttactac
     2881 cgtactttca cttccccaat taattggtag taatccgtgg gatgatgcaa cgcaatttaa
     2941 aattgatagc aatattgagc tttattttga tgaaacagta atggccggta gcggtgacat
     3001 cataattagt aatggagtcg atacacgaat cattccaatc aatgacacca atcaagtttc
     3061 ttttagtaaa gatggctcaa cgatcaccat caacccaacc gctgatttaa ttcctaacac
     3121 tgactacaat gttcaaatcg caagtggcgt gataaccaat ttagcgggca acccatacct
     3181 tggtattaat gacagtcgga ctctcaactt tacaaccatc ccttctaatc ctttactgat
     3241 cggtagctat ccttgggatg atagtagtga attccaggtc gatagtgata tcgttcttag
     3301 ctttgatgag ggagtaaaaa caggtattgg taatatcacg attacgaatg aagctgattc
     3361 aactgatagt cgggtaatct ctgtcaatga tgctactcaa gttctatttg atgattctgg
     3421 ttatgtcatc atcaatccta tggacaacct agcctccaat gctgaatata gtattcaaat
     3481 agactccggt gcaattatcg atctagctgg aaatgcatat gctggtatct ccgacaacac
     3541 atcgctcaat ttcacaacca ttccaacgaa tcccttgctg aactggagca acccaccaga
     3601 tgatttcaat catttccaag tagaaaataa catcgagctc ttcttcaacg aaagtataat
     3661 tgctggtagt ggcaatatca tcatcagcaa tggagtagat acacgcatca ttccaattac
     3721 agacactagc caggtcagct ttgatcaata ctatagcgct attgttacta ttaatccaac
     3781 agacgattta atacccaata ctacttacca tattgaaatc gaaagtggtg taattaacga
     3841 tttagcgggt gacccattcc taggcattag cgatgataca acactaaatt tcacgacgac
     3901 acttaaccca ttcttaatag gtagctcccc ctgggatgat tccaccgatt tcaaaattga
     3961 tgataatctt tggcttagtt ttgatgaaac cgtaaaagca ggtgttggaa atattacgat
     4021 cacgaatgaa accaatccag ctgatagtcg catgatctca gtagatgaca taactcaagt
     4081 atcatttgat gattttggta atgtcatcat taatccgaca gctgatttga ttccgaatac
     4141 tttatatagt attcaaatcg ataatactgc aatcactgat cttgcgggtc atgcttatat
     4201 tggcatctcg gataacacaa cgcttaactt cacgacgatt ccatcaaacc cacgcctatc
     4261 cttcagtacg ccagcagatg atacaattct ttttcctacg gatggaaata tcacattgag
     4321 ctttgatgag caaataacag ctggaattgg taacatcatc atcacaaatg aatcggatac
     4381 ctcagacaca cacacctttg caattaacga taccagccaa gttacgatca gtggcagcaa
     4441 ggtaacaatc gatccatcga cagacttgat gccaaataca acctacaaca ttcagattgc
     4501 cgaaggcgtt attaaagatc tcgcgggtaa tacttataaa ggtatccaag atgctgatac
     4561 gcttgatttt actaccggtg atgcgttcca aacctcgaca gtgggtatta acgaattcaa
     4621 tctacccatt cttccgctgg gttagaaagt aacacgtcta atttcgctga aatatccaaa
     4681 cctttagggt aagtcagcga aattaataat accctgttca caatgactac gtctcagtct
     4741 caaatttcta atttgttaca gaaagcggcg cattgtcatc agcaaggcaa tttccaggat
     4801 gcggaagcct gctgcctgaa agtttgtgca ttagtgccta accaacctga tacgctccat
     4861 ttacttgcca ttatttacgc gcaaacaaag caatacgaat tggcaaacaa ttattttatc
     4921 aaggcaataa ccgttgctcc tcaacgcgct gattttcttg gtaattacgc aaatgcatta
     4981 tgggaacaag gcaatattga cgatgcgtta attaactgcg agaaatcgct agcacttaac
     5041 tcgaatcaag cagatgtcca taatattctg ggtaatgttt atcttgcaca aaatcgtata
     5101 gaagctgcca ttggctgctt tcgaaacgct ttgcaatatc gccctaattt tccgcaggca
     5161 ttaaataatt taggtaatgc gcttcaaaaa ataaacaaaa tggaggaagc agttaaaagc
     5221 tattccaatg cgctcgggct aaatgaaaat tatccagaag cttacaataa tctcggccag
     5281 gcactcaaaa cactgggtcg catcgaggaa gcaagaaact attttctcaa agcaattgca
     5341 ctccgtccta actttactca agcaatctta aatcaccagg aagttgccat gatctggctc
     5401 gctccacttg agggaaacaa actttattta cgtcgctatt gtaaagaaga tgcaagctat
     5461 cttcgccaat gttatcaaaa tacccagttt atggggcaat acaatcacta tattccccgc
     5521 catcaacgcg cagaggagct tgccttaaaa ttagacgaag cgaataaaat gcatccttgc
     5581 caacttaaat ccattgattg ggtcattatg aagaaaggag cgaatcaacc tatcggtatc
     5641 gcaaatctgg ttggtattga gtttgctcac cgccgtgccg agtttcttat tggcttgcca
     5701 gatacaaaca atcacgcaaa agggttaggg ctggaagcga ctttattagt tctcgatttt
     5761 gcttttaata aagtcaattt gaacaaaatt acgtctatcg tttatgaaga taataccgta
     5821 tctcaacaaa acgctttagc cttaggtttt atccaagaaa gtatgttacg tgatcatatc
     5881 tttgattcaa cgagtggaaa atttctgaac ctctggggaa tcggcatgac catgaatgat
     5941 tttcgcaata atctgcgcat tgctaagtta tcggatcggc ttttaggtaa aaatattacg
     6001 cttgtgcctt gataagatcc aactcatctt atccatatct aaccgtttat ctggctaaat
     6061 cgtagttaat taggcagaag ctataggaaa attattagaa tgatgaagca cagtctgatt
     6121 tgaagagact gcagtatctt taacaagtag cgctagtttt attcgatcgg taattgctaa
     6181 tttacgaaaa atttctgtta gatgggcttt aacagtacgc tcggttatat ctaatcttct
     6241 cgcaatctgt ttattgcttt caccacgacc gaccaagata gcaatttcat attctcgctt
     6301 cgttaaattt tcaagcagat tattaacagc acgctcaata tgttttttct cttttgtggt
     6361 aaccaccagc tcatccaaca tatgatgaac cagcttgcgc cgaatccaca attctccttg
     6421 ccaaacagct ttaacaactc gctttatttc tccaggttca atcgaatcac gataacatcc
     6481 cctgattccc gacttaaata atttccattc catttcatct gaaatacctg ggcttgatat
     6541 aatgatttga gttttagggc ttaaggcaag taaaccctct attttctttg aaccatcatt
     6601 ccctaagaag tcatggtcta ttagaagtac tggcggtttt cttctcgcta attcatggtt
     6661 cagcaattca tgatcattga tgcaaagaac ggacgaaaca tcagttaagc ttcttctcca
     6721 ttcatctagc atttcttcat atgaacttgc aagcaatatc acaattggct cccttgaatt
     6781 aaactaagtt tagaatgtca gtattaacga cattatggtc agtggcttaa ttcaatcggg
     6841 aaattaaatt aacccctaat tttattttta tcgaggtaga aattaagcaa aaaaccactc
     6901 taaatctcta ggtgcaccga ctattatttt gcaagagaat atttaaatga ctcgattatt
     6961 tcgaagcttt cccctagtgt taaaaatctc cagtacattg caaatgttac ttgggttagt
     7021 tctgctaatt attggactgt ctatttcaca tgcagaaaat ttactgaaat cagagactat
     7081 cattgcccat ttcgaggaaa agcaaagtag cactcgaaat tttaacgaga atatcaccgt
     7141 gacaatcttt gcagatggtt acgtaaaagt atatcgtcct agttattata aaaaatcagg
     7201 gatattctct ggacaccttg atccagttgc gttgaatcaa ttgtggaatc aattaacctc
     7261 gccaagccta cttaatttca atgaaactga gattcagcat cgtctgaaaa ctgctaaaaa
     7321 tcaacaggaa aaaaatacgt tattgctaca acgtgtttca gatgcgccta caatcatcat
     7381 agaaatttat ccaaaccgat atatgccaac tgccagtatt gatcaaaaca attggaatac
     7441 gacaaagcga atcacttggt acgcactaca atggacagta gaaaactttc cagaattaga
     7501 agaactgcac gaattgatgg aagttagaga actatttgag aatattctgg aatcttctga
     7561 gctaaagtcg cttaattaat cctttttacc aatagtcgtc gaagtacata atgaaggaca
     7621 tcagcacgat cattttacta atatttacct atgggctaat gctgaatacc gtagccggta
     7681 ctttcttatt tgcagaagat aatgaaacag cagacgttat cacccaccct caaggttatc
     7741 agggtcaagg aacaaatcta atcgttacag tcggcatctc accttcttca cttcatggca
     7801 ctgaaatagc actaccgatt caaaatgcaa ttaatacttg gaatcaatta attcccacgc
     7861 ttggcaatat cgttagcgat gaaagaattc cgttcaatca tttcgatttt gaatccgtgg
     7921 tattacatga gctcgggcat tgcttaggtc ttgcacatcc caatttatct tctgaatcag
     7981 ggctttcggg agaaaatcgt aactataccc aaaccactcg gggctcaaat aaccgatacg
     8041 atctgaatag tggaagcgat ggtatcgctg gctcaaatga tgatttacga ggtgatgatg
     8101 caaacttgca ttggtttagg agaaaaaata ataacccttt tactatcgca gaaaaaattg
     8161 atattaccac ttatagtcgg aatttaagtg atttaccggt agggcatacc ttcgctgcca
     8221 atggtgatcg aactgtagcg aatctgcttg gttaccaaaa tactgaagcc gttatgcagc
     8281 agggtatcat cgctggagaa acacaacgaa ccctcacagc agatgatgtt gcaacattaa
     8341 aacttgccat gagtgggcta gatagatttg ctggcacacc tgatgactat acgttagctc
     8401 taacgttcat tggatttacg gatgatgcag atattgtcgt cagcttcaat aacagtcgtt
     8461 catcgtttgc agtttgcgaa acccgagctg aacaactgaa ttcaaaacat tttgcgatta
     8521 cccggagtag aatctatttc aatacgaatg tgcaatggta ctttaatgac ttaacttcac
     8581 ccaatccacc gaaactctca tatccgagtc ctacgattgc agcaaataat caaacgggaa
     8641 ccttacaact ttccagaaac gatacacttt tactgacagt atctttagat ccgggggtac
     8701 ttctagaaaa ccctgcagag tattggatac gtgcgatcac cccaaccgaa actttctggt
     8761 taaatgatcg attcgaattt attccttctt cacaaccgat acgggcgcat gttggagaat
     8821 tcaatacgat caatcaattc attatcatga aaagctcact caaagcccta caaccgggta
     8881 attactctat aacgtttgct gtcgatgaca atcaagacgg tattgccaat ggcacctttc
     8941 acaataccgt caatattacg atcacgccat aaactcatca aagattaaca ctgatcgcaa
     9001 ttagatctca gcattacaca gtattaatca tcacggtcct cgtctttatc acgaccgttc
     9061 tttttcccat gatgtttccc gtaattgcgg ttgataactc caaaacgatc atcatcactt
     9121 cgctttgatt cataatgatg ataatcacga gaatatccat tccgatcatc atctacaata
     9181 attttttcag cgataacatc tagcttctga ttaagataac cttcaacttc aacaaattca
     9241 ccttctgcta ggcttccata gattttggta gttcctgttc gaaccacgat caaatgggct
     9301 tcatgaatcg ttacaatttc aatgatgtca ttggtaagat caataatttt tccttttacg
     9361 cgcacatgct catctggacg cttaccaact tcaacgcgat ccgcaaaaaa tgtgtcatcg
     9421 agatatctac ctttcacatc gacactttca ccgacttgaa gcgcagatat aggcagattg
     9481 ttaaccacgc ccagtcctcg actccgaatt gaggtggatt catcgactag aatatcaata
     9541 cctaacacct gcataaaaat gccatcttcg acaggctcaa cccccttaat taaaccgcgt
     9601 aagcgaaact tacctaatcc accatctagc acgtaaattg attctgcaac aatgccatct
     9661 tcaccaaaga aaccgttaat ttttacgaca tcaccaactt ctaattcaga caagggtata
     9721 tggtaaccat tcgactcaat ctctgtggca cctaacacaa tgataggcac ttcaaaatca
//
LOCUS       DAOQKQ010000098         9774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_3287_length_9774_cov_6.297150, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000098 DAOQKQ010000000
VERSION     DAOQKQ010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 9774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9774
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3287_length_9774_cov_6.297150"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /gene="dapE"
                     /locus_tag="PKZ49_07650"
     CDS             <1..1015
                     /gene="dapE"
                     /locus_tag="PKZ49_07650"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HND36473.1"
                     /translation="EKLNFGDVENLWARRGTKQPLVCFAGHTDVVPTGPVGEWESDPF
                     VPTIRDNRLYGRGAADMKASLAAFITAIESFLEANPEPEGSIALLITSDEEGPAVDGT
                     VKVVEILKERGESIDFCIVGEPTCTDQFGDTIKNGRRGSLSGHLTIKGIQGHIAYPHL
                     AKNPIHLAAPVIAELAQTVWDNGNEYFPPTTWHISNIQGGTGATNVIPGHISLIFNFR
                     FATASTPESLQKKFCEILDKHGLEYQITWELSGKPYLTPRGKLADALTEAIKTVTTIT
                     PQLSATGGTSDGRFIAEICPQVVEFGPRNATIHKINESVEIEDIERLSQIYQKALEYL
                     LGH"
     gene            complement(1043..2131)
                     /gene="hemH"
                     /locus_tag="PKZ49_07655"
     CDS             complement(1043..2131)
                     /gene="hemH"
                     /locus_tag="PKZ49_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HND36474.1"
                     /translation="MMLPEPPFQHGTSSKTGVLLINLGTPDAPTAEALKPYLRQFLSD
                     SRIIELPRWIWWFILNGIILNTRPKKSAEKYALVWTEEGSPLKVHTERQTKLLAQALA
                     SSISPVPMVEYAMNIGNPSIAHVLAKMKAAGCDQILVLPLFPQYAASSIGSAMEHVFA
                     ELAKIRNMPAVRTIKHFHDDHGYIAALAQNVQDYWNSHGKPSKLVISFHGVPRKTLDK
                     GDPYHCECQKTGRLLAEALALKPEEYQVCFQSRFGKAKWLTPYTTDILQELGKQKLNR
                     VDVVCPGFVADCLETLEEIAIEGKKTFVEAGGNEFHYIPCLNEHPAWINALTQITRNH
                     LQGWLAPEPTAEEAALCRERATKLGASS"
     gene            complement(2200..3222)
                     /gene="hrcA"
                     /locus_tag="PKZ49_07660"
     CDS             complement(2200..3222)
                     /gene="hrcA"
                     /locus_tag="PKZ49_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HND36475.1"
                     /translation="MLNDRAKSLLKTLVERFIVDGEPVGSRSLSKFSGLDLSPATIRN
                     VMVDLEEMGYIASPHTSAGRIPTALGYRFFVDSLLVVKTLESHEVNHLENQLHPDNPS
                     RLINVASQLLSELTRFAGVVVTPKRSGSAVFRHIEFMTLSEKRVLLILVTPEGDVQNR
                     IIFTEASYSQTDLIEASNFLNQHYAGCTLEEIRIRLQTELKQLRHEMMLLMNAAINVS
                     NDVIQESSEAVVVAGEHKLLDVKDLSDNLSNLKQLFELFERKTKLLQLMDLSRQAHGV
                     KVFIGGESNVAALDEVSVITAPYEIEGAIVGTVGVIGPRRMAYERVIPIVDITARLLS
                     SSLSQH"
     gene            3430..4314
                     /locus_tag="PKZ49_07665"
     CDS             3430..4314
                     /locus_tag="PKZ49_07665"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HND36476.1"
                     /translation="MSTRFNKIALIGKHKNPEILAPMMKLAEYLASRQFGVIIDRLSA
                     AHLEHSEFPILTLEEIGAEADLAVVIGGDGTMLNIARILAPYNVPLIGVNQGRLGFLT
                     DLTVDTMIETLGEMLDGQFMIEQRVLLSAEVIRQDQVVYKALAFNDIVIHRGISTGMI
                     EFQVRINGEYVYSLRSDGLIVATPTGSTAYALSAGGPILHSSLDLIELVPVCPHTLSN
                     RPIVVGPDAEIEIMLNYNTETKIYSDSHSWFDLGEHDRILVRRFPQVVKLLHAINHSY
                     YRMLREKLGWSEIPHKNH"
     gene            4323..5999
                     /gene="recN"
                     /locus_tag="PKZ49_07670"
     CDS             4323..5999
                     /gene="recN"
                     /locus_tag="PKZ49_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HND36477.1"
                     /translation="MLTHLTIQNFVIVERIELSLEPGFTVLTGETGAGKSILIDALML
                     ALGGKSDTNQIRQGCERAEIIAEFNIEHLPDTQRWLQENDLADDSGSCILRRVIEANG
                     RSRHFINGISVALQQLRAIGDQLVDIHGQHAHQSLLRSDAQRALLDGFAGNDELLAQV
                     KAAYQHWHGLNQQRLAWEANQSAFAQEKEQLAWQVDELTSLNFNPQEWQSLQADHQRL
                     SHSASLQAAVEYSIDQLSEGEAALLGQLQTTLTRLRQALDYDEKLQSPYQLLESTSVQ
                     LQEGIHELKHYQKRLDSDPQRLQDVEERIAAIHAMARKYRCDIESLPERLQLAHDRLQ
                     VICGEYSGDNLIEAEASARQHYEAVAQQLTLSRAGAAQSLAKQVTQSMQNLAMAGGIF
                     VINLIPVEQGNANGFEQIEFQVSPHKDTPARPLTKVASGGELSRISLAIQVITSKVSR
                     VPTLIFDEVDAGIGGRIAEMVGTLLKQLGSAHQILCITHLPQVAAMGDQHWLITKIKD
                     AKHAQGISSHIDDLDQQARVEEIARMLGGATITTITRQHAAEMLQKCQNR"
     gene            complement(6011..7567)
                     /locus_tag="PKZ49_07675"
     CDS             complement(6011..7567)
                     /locus_tag="PKZ49_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HND36478.1"
                     /translation="MQPTQHNSFSNFWLYCTVFLTGAAVMIIELLGTRLIAPFYGASL
                     YVWASLISVTMIALAMGYFLGGYWADRAKHAGLALIIGLAALLTMTIPWLVQPVLLST
                     DHLGLKLGTFISTLILFTPSLTMLGMVGPFAVKRITANLAGVGTSTGSIYAVSTVGSV
                     IGTLFLGFYLFPQIGSREIIIGLAVGLFVLAFIVAIFERHQIPLIKTLSFVACFFFMG
                     VSILLATTPLSNERVNQQDNDYQTRFERESLYGWVRVIDNPSLNLRLLTADASTIGAA
                     SISQGENVLTYQKIVKMIPNLVPNMKQALLIGQGAGHMATTLERKGIRTDTIEIDPAV
                     AEAARDYFDFIPTGQTLIGDARYEIRKLKGPYDLVILDVFTGGSEPVHLLTFETLEQI
                     KALLTPDGLLALNFVAFFEQGQNTALASVAKTLAQSFAHQLAFISEPDHEFNDFIFIA
                     SRQPLTIEQSLLNTQQTIWLKQRRIEIDQRKGIVLTDNFNPLEHLQGNKSEHYRRMMV
                     DMIGTQFFIR"
     gene            8082..8825
                     /locus_tag="PKZ49_07680"
     CDS             8082..8825
                     /locus_tag="PKZ49_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36479.1"
                     /translation="MKQMTKARALVLSAVSAALVGMGMSAFAHTRLDIPTMTEGSRTI
                     NHLVGHTCGSNKAIGFSVVFPDGVDSTVLVNSQPHTGALTDFVEGYGNNAQLIFDRSQ
                     FDIVDEKMDPSGANVVGFWGGGGPGVPSNLTVATRFRLTAPTIVPTSCAKSVKAYISI
                     AVVCQVTGTDQFGTEGVVDLWTPNNLGTPFDSADANADAPWTISRDLTKNPLPESCGE
                     GVDVEIKPSAAQINRDMPVKVNGQQVWPQ"
     gene            8908..9390
                     /locus_tag="PKZ49_07685"
     CDS             8908..9390
                     /locus_tag="PKZ49_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36480.1"
                     /translation="MKQSTVFNVRKLVKQCITVLALSGGMLGVIHTGLAHDAAATMDS
                     VGNSATFTGYGFVTCFDDGNGPADKLVASVRDHSPPVPGLLVNLQILKGNRAASVSDP
                     VSGDGNGSPSISVKGGNGVYLILVNKTAPGARDFSIEYHCTTANDVHTGTDIGVYQFE
                     "
     gene            9443..>9774
                     /locus_tag="PKZ49_07690"
     CDS             9443..>9774
                     /locus_tag="PKZ49_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HND36481.1"
                     /translation="MKISNKFYGLTLAVTLTCFAVSPSFAHLEGDVPPPQCKLTALDG
                     KKVEDWSTLKGEVVYVDFWASWCPPCIQSFPFMTQLMDEFKEKGLHVIGVNLDENSQD
                     AINFLKQHP"
BASE COUNT         2750 a         1982 c         2306 g         2736 t
ORIGIN      
        1 cgaaaaactc aattttggag atgttgagaa tttgtgggcg cgtcgtggca caaaacaacc
       61 gttagtgtgt tttgcggggc acacggatgt cgttcctaca ggacctgtgg gtgagtggga
      121 aagcgatccg tttgtgccga ccatccggga taatcgatta tatggacgcg gtgctgccga
      181 tatgaaggcc tcattggctg cctttattac ggcgattgaa tcgtttttgg aagcgaatcc
      241 tgagcctgaa ggatcgattg cgttattgat tacctctgat gaagaagggc ctgccgtcga
      301 tggaaccgtc aaggttgtcg agattctcaa ggaacggggt gaatcgattg atttttgtat
      361 tgtcggtgag ccgacttgca cggatcaatt tggggacacc attaagaatg gccgacgcgg
      421 ttctttatct ggccatttaa cgatcaaagg gattcaaggg catattgcgt atccgcactt
      481 ggccaaaaat ccgatccatc tcgccgcacc tgtcattgct gagctagcac aaaccgtgtg
      541 ggataacggc aatgaatatt ttcctccgac aacctggcat atttccaata tccagggagg
      601 tacaggagca accaatgtga tacctggcca catcagttta atatttaatt ttcgttttgc
      661 aacagcgagt acgccagaat cgctacaaaa gaaattttgc gaaattcttg acaagcacgg
      721 tcttgagtat cagataacat gggagttatc aggtaagcct tatttgacac cgagaggaaa
      781 attggccgat gctttaacgg aagccattaa aactgtaacc acgataacac cacagctttc
      841 tgcaacgggt ggaacctcag acgggcgatt tattgcagaa atctgtccac aagtcgttga
      901 attcggtcca cgtaacgcga cgatacacaa aatcaatgag tctgtagaaa tagaagatat
      961 cgagcggttg tcacaaattt atcagaaagc cttggaatat ttgctcggtc actgagccaa
     1021 tattctggtt tggctagcat gactagctgc ttgcacccaa tttggtggcg cgttcacgac
     1081 aaagtgcagc ttcttcagca gtgggttctg gcgctaacca accttggaga tggttccgtg
     1141 tgatctgggt tagtgcgtta atccaggcgg gatgctcatt gaggcaaggg atataatgaa
     1201 attcatttcc acctgcttca acaaaggttt ttttgccttc aatggcaatt tcttctaagg
     1261 tttctaagca gtccgcaaca aatcccgggc aaaccacatc gactcgatta agtttttgtt
     1321 tgccgagttc ttgaagaata tcagtggtat aaggtgttag ccacttagcc ttgccaaagc
     1381 gtgattgaaa acaaacttga tactcttctg gctttaatgc taaagcttcg gccagtaatc
     1441 ttcccgtttt ctgacattcg caatgataag gatcgccctt gtccaacgtt tttcgtggga
     1501 caccatgaaa gctaatgacc aacttgctcg gcttaccgtg gctattccaa taatcctgta
     1561 cattttgtgc caaggctgca atgtagccat gatcatcatg gaagtgctta atcgtccgaa
     1621 cagccggcat attgcgaatt ttggccaatt cagcgaaaac atgttccatc gcagaaccga
     1681 tgctactcgc tgcatattga ggaaacaggg gtagcactag aatctgatca catccagccg
     1741 ctttcatttt tgctagaacg tgtgcgatag aaggattacc aatattcatt gcgtattcca
     1801 ccatgggaac cggagagatg gatgaagcta atgcttgggc aagtagctta gtttgtcgtt
     1861 cggtgtgaac cttgagggga gaaccttcct cggtccatac taatgcatat ttttctgctg
     1921 atttcttggg gcgcgtattg aggataatgc cattcagaat gaaccaccaa atccagcgag
     1981 gcagttctat aatgcgagaa tcactcaaga attggcgtaa gtaaggtttt agcgcttctg
     2041 ctgtgggtgc atccggtgtt cctaaattga ttaataacac accggtttta ctcgatgttc
     2101 cgtgctgaaa ggggggttct ggtagcatca tgatttattc ttgtgatgtg ttgagtagca
     2161 atagtgattg ttttaaaggc gatttatcgt gaaatcaaat cagtgttgcg agaggctgct
     2221 tgaaagtaat cgtgctgtga tatcaacaat aggaataacg cgctcataag ccattcgtct
     2281 cgggccaatg acacccacag tgccgacgat tgcgccttcg atttcatagg gtgcagtgat
     2341 aacactgact tcatccaatg ctgcaacatt cgattctccc ccgatgaaaa ctttgacacc
     2401 atgtgcctgg cggctgagat ccatcaattg taataacttg gttttacgtt caaataattc
     2461 gaacaattgt tttagattgg agagattatc cgagagatct ttcacgtcga gcaatttgtg
     2521 ctctccagcc accacaacgg cttcgctact ctcttgaatg acatcgttac tgacgttaat
     2581 cgcagcattc atgagtagca tcatttcatg acgaagttgt ttcaattctg tttgcaaacg
     2641 gatgcgaatc tcctcaagcg tacaacctgc ataatgttga ttcagaaaat tactggcttc
     2701 aatcaaatcg gtctgactat aactcgcttc tgtaaaaatg atacggtttt gtacgtcgcc
     2761 ctctggtgtg accagaatga gcaatacgcg cttttcagac agcgtcatga attcgatatg
     2821 gcggaaaaca gcgctaccgg aacgttttgg ggtaacgact acacctgcaa atcgcgtcaa
     2881 ttcagataat aattgagaag caacattaat caatcgagaa ggattgtctg gatgcaattg
     2941 attttcaagg tgattcactt catggctttc taatgtttta acgaccagta agctatcgac
     3001 aaaaaatcga taaccgagcg cagtcggaat gcgtcctgcc gaagtatgcg ggctcgcaat
     3061 ataacccatt tcttctaggt caaccatgac attgcgaatc gttgcaggac taagatccaa
     3121 tcctgaaaac tttgagagcg aacgcgaccc tactggctct ccatcgacaa tgaagcgctc
     3181 cacgagtgtt ttgagtaatg attttgcacg atcgtttaac atatcagttg atattttaca
     3241 cgattaaact tataccgcgc gtaagttaac ccctgcttat ccatggtatt tttatgttat
     3301 ccctttgttt gtgttgtcac cgcattatcc ttttaccttt ccagttgact acgttaatat
     3361 tcaaaataat cacattacct gtgtcagtgt attaatagat atgtattgcc ctaatttgtt
     3421 tcaataacaa tgagcactcg ttttaataaa attgcattaa ttggcaaaca taaaaatcca
     3481 gaaattctag cgccgatgat gaagctcgcg gaataccttg cgagtcgaca atttggcgtc
     3541 attattgatc gattaagtgc agctcatttg gagcattccg agtttcccat tttgactttg
     3601 gaagagattg gtgccgaggc tgatctggcg gttgtcattg gtggggatgg gacgatgttg
     3661 aatattgctc gcatattggc gccgtataat gttccattga ttggggtcaa ccaaggtaga
     3721 ttgggttttc taaccgattt aaccgtcgac accatgattg aaacactagg agaaatgctt
     3781 gatggccaat ttatgattga gcaacgggtg ctactttcag cggaagtcat tcgacaggat
     3841 caagtggttt ataaagcact ggcttttaac gatatcgtta ttcatcgcgg gataagtacc
     3901 gggatgattg aatttcaggt acgcattaat ggtgaatatg tttattcatt gcgttcggat
     3961 gggttgatcg ttgcgacccc aacgggatcg actgcttacg cactttcagc agggggaccg
     4021 atattgcatt ccagccttga tttaatcgaa ttggttccag tttgtccgca taccttgagt
     4081 aatcgtccca ttgtcgttgg acctgacgca gagattgaga tcatgctgaa ttacaatact
     4141 gagactaaaa tttactcaga tagtcattct tggtttgatc tcggtgaaca tgatcggata
     4201 ttggtaaggc gttttcctca agtcgtcaaa ttgctacacg cgattaacca tagctattac
     4261 cgcatgctgc gtgagaagtt ggggtggagt gaaataccgc ataaaaatca ttgattgtca
     4321 gcatgctgac ccatttaacc atacaaaatt tcgttatcgt cgagcgcatc gaattgagtc
     4381 tcgagccggg ttttacggtg ttgacgggag aaacaggggc aggaaaatcc attttaatcg
     4441 atgcattaat gcttgcatta gggggcaaaa gtgataccaa tcaaatccgg caaggctgtg
     4501 aacgtgctga aatcatcgct gaattcaata ttgagcattt gccggatact caacgttggt
     4561 tgcaagaaaa tgatctggcg gatgattcag gaagttgcat tttacgacgc gtcatcgaag
     4621 cgaatggacg gtcacgccac tttataaatg gtatttcagt tgctttacag caattacgcg
     4681 caatcggtga tcaattagtc gatattcatg gacaacatgc gcatcaatcg ctattacgaa
     4741 gtgatgcgca gcgcgctttg ctcgatggat ttgctggaaa cgatgaatta ctggcacaag
     4801 tcaaagcagc ttatcagcac tggcatggct taaatcaaca acgccttgct tgggaggcta
     4861 atcaatcagc gtttgcacaa gaaaaagagc aattggcctg gcaagtggat gagctgacta
     4921 gccttaattt taatccccaa gaatggcagt cattacaagc cgaccaccaa cgtttatcgc
     4981 atagcgctag cttgcaagca gccgttgaat attcgattga tcaattatcg gagggagagg
     5041 ctgcgctatt gggacaactt caaacaacac tgacccggct tcgtcaagct ttggattacg
     5101 atgagaaatt gcaatcccct tatcagttac tggaatccac atcggtgcaa ttgcaagaag
     5161 ggattcatga gctgaaacac tatcaaaaaa ggctcgactc agatccacag cgtttgcaag
     5221 atgttgaaga gcgcattgct gcgattcatg ccatggcgcg aaaatatcgc tgtgatatcg
     5281 aatcattgcc tgaacgactt cagttggctc atgatcgttt gcaagtgatc tgtggagagt
     5341 actcgggtga taatttgatc gaggcggaag ccagtgctcg acagcactat gaagcagttg
     5401 cccaacaatt gacactttca cgcgctgggg cagcacaatc gcttgcaaag caagtgacac
     5461 aaagcatgca aaatttggcg atggcggggg ggatttttgt aatcaatctg atcccagtcg
     5521 agcagggtaa tgcaaatggg tttgaacaga tcgagtttca agtatcaccg cataaagata
     5581 ccccagcccg ccctttgacc aaagtagcat cagggggtga gctatctcga atcagtttag
     5641 cgatacaagt cattaccagc aaggtaagtc gcgttcctac actgatattt gatgaagtcg
     5701 atgctggaat tggtggacgt atagccgaga tggtaggcac attacttaaa caattaggaa
     5761 gtgcccatca aattttatgt attacccatt taccccaggt tgctgcgatg ggagatcagc
     5821 attggctcat taccaaaata aaggatgcaa aacacgctca aggaatctca agccatattg
     5881 atgacttgga tcagcaagca cgtgttgagg aaattgcgcg aatgctggga ggcgcaacga
     5941 ttacaacgat tacccgacaa catgctgcag aaatgctgca gaagtgccaa aacaggtaat
     6001 aattgacttg ctagcggata aaaaattggg ttccgatcat gtctaccatc atccttcggt
     6061 aatgttccga tttgttacct tgtagatgtt ccaaaggatt aaaattatcc gtcagcacaa
     6121 tgcctttacg ctgatcgatt tcaatcctac gctgttttaa ccatatcgtt tgctgggtat
     6181 tcagcaaact ttgctcaatc gtgagtggct gcctgcttgc gataaagatg aaatcattaa
     6241 actcatgatc gggttccgag ataaacgcaa gttgatgagc gaagctttgt gcgagggttt
     6301 tcgcaacaga agctagcgcc gtattttgcc cttgttcaaa gaatgcaaca aaattcagcg
     6361 ctaagagacc atctggggta agtagcgctt taatctgttc aagcgtttcg aatgttagta
     6421 gatggacagg ctcagatcct ccggtgaata catcgaggat gaccaaatcg taggggcctt
     6481 taagctttcg gatttcatag cgggcatcgc cgatcaatgt ttgccccgtt ggaataaaat
     6541 caaagtagtc acgcgctgct tctgcgacag caggatcgat ctcaatggtg tcggtacgaa
     6601 tacctttgcg ttcaagggta gttgccatat gtcctgcacc ttgtccaata agcagcgctt
     6661 gtttcatgtt aggcacaaga tttggaatca ttttgacgat cttctgatac gttaatacat
     6721 tctcaccctg actgatactg gctgcaccga ttgttgacgc gtcggcggtt aatagacgta
     6781 ggttgagtga aggattatca atgaccctta cccacccata caagctttca cgctcaaagc
     6841 gtgtttgata atcgttatct tgctgattga cacgctcatt ggataacggt gtggttgcca
     6901 ggagtatcga tacccccatg aagaaaaaac aggctacaaa cgatagtgtt ttgatcaatg
     6961 gaatctggtg tcgttcaaag attgcgacga taaaagccag cacaaaaagc ccgactgcga
     7021 gaccaatgat aatttctctt gagccgatct gtgggaataa ataaaagccc agaaacagtg
     7081 ttccgatcac gctacccacg gtgctgaccg cataaattga accagtactc gttccaacgc
     7141 cggctaaatt ggctgtaatg cgtttaacag caaaagggcc aaccataccg agcatggtga
     7201 gactgggggt aaaaaggatc agtgtgctaa tgaaagtacc gagttttaat cctaagtgat
     7261 cggtgctgag taacaccggt tggactaacc acggaatcgt cattgtgagt aaggctgcta
     7321 atccaataat caacgctaaa ccagcatgct tagcacgatc tgcccagtac ccacctaaaa
     7381 aataacccat tgccagtgca atcattgtta ccgaaatgag tgaggcccaa acatataagc
     7441 tggccccgta aaaaggggca attaagcgtg tccccagtaa ctcaatgatc atgactgcgg
     7501 cgcctgtcaa aaaaaccgtg cagtacaacc agaaatttga gaaagagtta tgttgagtgg
     7561 gttgcatgat ggtattgctt atcagattgg gagcgtcatt gtaaagttga ttgcggtaat
     7621 tttaaatgcg acaaattgtc gcacttgagt attttggagt tgaccaaaat attgtgagtt
     7681 tgctagttga atgagctact tattcaaatg attattaact ggagtatctt tgcagaaggg
     7741 gcgatgcggc aatttgtcac attgctattt tttttcattg ccagaataat ggttcgcaac
     7801 aaattactaa aaaaattata gttattctat aaatttaacc aaagtttggc acaaagaatg
     7861 aaattcagct cacctgtttt attaacattg cagattaacc tccctcctac acaggcatgg
     7921 attatccttg cacccattgc ctgtgtagtt tttttataag ggtgcgacaa tttgtcacat
     7981 tgttaagcaa ggtgcaaaag cagataatcg aaacaacctt attcgcgaat ggttaataaa
     8041 agagttaaaa atgaatgata aaaatttgat gagggaaaaa aatgaaacaa atgactaaag
     8101 caagggcgtt agtactttct gcagtttctg ccgccttggt tggaatggga atgagcgcgt
     8161 ttgcacatac acgattggat atacctacca tgacggaagg gtctcggaca attaatcatc
     8221 tggttggaca tacctgtgga agtaataaag caattgggtt ttcagttgtt tttccagatg
     8281 gagttgactc tacagtgtta gttaatagtc agcctcatac gggcgctctg actgattttg
     8341 tggaaggtta tggaaataat gcgcaactta tctttgatcg gagtcaattt gatatagtgg
     8401 atgaaaaaat ggatccttca ggtgctaatg tagtgggatt ctggggaggt ggtggtcctg
     8461 gtgtaccgag taatcttacg gtagcgacac gatttagatt gactgcacct acgattgtac
     8521 caacgtcttg cgcgaaaagt gtaaaagctt atatttcaat tgcggttgtg tgccaagtaa
     8581 ctggtacaga tcaatttggt acagaagggg ttgtagattt gtggacgcct aacaatttag
     8641 gtactccttt tgatagtgcg gatgcaaatg cggatgcgcc atggacgatt agccgagatt
     8701 taacgaaaaa tccactgcct gaatcctgcg gagagggggt agatgttgag attaaacctt
     8761 ctgcagctca aattaatcgc gatatgccgg ttaaagttaa tgggcaacag gtatggccgc
     8821 aataaccaat taattgacct aaaggaacag aaagtataac cctgatgagt tatactttct
     8881 ggatcgaatg atagaaggat gatcgtcatg aaacaatcta cagtgtttaa tgtaaggaag
     8941 ctagtcaagc aatgtattac cgttttagct ctttcaggtg gaatgttggg ggttattcat
     9001 acaggattag cacatgatgc ggcggcaacc atggattcag ttggcaatag tgcgacgttt
     9061 accggttatg gattcgtcac ctgttttgat gatggtaatg gtcccgcaga taagctagtt
     9121 gctagcgtta gagatcactc tcctcccgtt ccagggttat tggttaatct gcaaatcttg
     9181 aaaggaaatc gtgccgctag tgtgagcgat cctgtgtcgg gtgacggaaa tggtagtcca
     9241 tcgattagcg tcaaaggggg gaatggggtt tatctgattc tagtcaacaa aacagcccca
     9301 ggtgcacgtg atttctcgat tgaataccac tgtacgactg ccaatgatgt gcatacggga
     9361 acggatatcg gtgtctatca gtttgaatag cgcgatagat taccgcataa tgtcacattg
     9421 gttttaatag cgaataataa caatgaaaat atccaacaag ttctatggat taacgcttgc
     9481 ggttacttta acttgttttg ctgtatcgcc gagttttgca catcttgaag gcgatgttcc
     9541 gccaccgcaa tgtaaattga ctgcattaga tggaaaaaaa gttgaagatt ggagtacttt
     9601 aaaaggggaa gtggtttacg ttgatttttg ggcttcctgg tgtccccctt gtattcaatc
     9661 gtttcctttc atgacacagt taatggatga gtttaaagaa aaagggctac atgtcattgg
     9721 cgtcaatttg gatgaaaatt cccaggacgc tataaatttc ctcaaacaac atcc
//
LOCUS       DAOQKQ010000099         9763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_3296_length_9763_cov_6.604347, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000099 DAOQKQ010000000
VERSION     DAOQKQ010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 9763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9763
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3296_length_9763_cov_6.604347"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1473
                     /locus_tag="PKZ49_07695"
     CDS             <1..>1473
                     /locus_tag="PKZ49_07695"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HND36482.1"
                     /translation="EQVIADLFAQSVHKRDYLQLTPSERQALLLDEITSPRTLYSPYF
                     KYSATTQSELRILKMAAEIQHRFGHAALPNYIISMTTSVANILEVVLLLKEVGLLHSG
                     ENPRLGLNIIPLFETIGDLRGCGAMMDELFSVPYYRKLLESRGNEQEVMLGYSDSNKD
                     GGFLTSNWEIYKAEINLTEVFAKHQIELRLFHGRGGTVGRGGGPSYQGIMAQPPHSVN
                     GQIRVTEQGEVVASKYTDPEVGRRNLETLVAATIEATLLSHETIQADVTRYHQAMETL
                     SSHAFSAYRNLVYETPGFKQFFLESTPVREIAGLHIGSRPASRKPSDRIEDLRAIPWV
                     FSWSLNRTIIPGWYGFGSAVEKFIQQHEQDETGLKLLQEMYAKWPLMQTLLSNMDMVL
                     AKTDLGIASRYAELVTDVSLREQIFSRIQEEWQRSVKWLFQITGHSELLEDNPTLARS
                     IQIRTPYIDPLNHLQIELLRRYRAGGDVEEQVHRSIHLTI"
     assembly_gap    1474..1573
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1579..2946)
                     /locus_tag="PKZ49_07700"
     CDS             complement(1579..2946)
                     /locus_tag="PKZ49_07700"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HND36483.1"
                     /translation="MAINEKATTFTYGTRKNDTIDAQYLLGDMIDGLQGDDTITGLSG
                     NDTLLGGSGRDTIFGLDGNDLLDGGAGNDKLYGGAGDDLIRPDTGAIGSDLIDGGDGI
                     DTLDYSVSAAVSGVTVDLRKIVLQDTKGAGKEIILNIENINGTNFVDTLTGNDTRNVL
                     VGGNGGDTIYALDGHDQIDGGAGNDKLYGGLGNDLLIPDTGIIGNDSIDGGDGIDTIN
                     YQRQGLISGVTVDLSLITSQNTGGAGIDLILNVEQVQGSDFNDTITGSNLDNWLNAGM
                     GSDIINGGDGNDAIFSGAGDLLGDSLYGGNGDDKIRGGTGNDVLDGGSGADLLRPNRG
                     ADSLTGGSDSDTFIFTISSSGTGSDSNPIAPDLITDFNGLEDTIILQSTEWSPNSVVF
                     LGNAPFTASGVSEVRLTYDLGLGTQRIEVDIEGNGSADIDSVINVIGTPIVADDLVFV
                     STYPF"
     gene            complement(3204..3368)
                     /locus_tag="PKZ49_07705"
     CDS             complement(3204..3368)
                     /locus_tag="PKZ49_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36484.1"
                     /translation="MMIKKRITTYVLALMVAMAFSSAFANPPQTPEDCQKIYAGDDVK
                     VRTCIDSLKK"
     gene            complement(3762..5417)
                     /locus_tag="PKZ49_07710"
     CDS             complement(3762..5417)
                     /locus_tag="PKZ49_07710"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HND36485.1"
                     /translation="MSETKSIHLLINWCKPYRVFFWLVAAILLIVGVSSATLPLVAKA
                     LLDHMLDQDQETMMVTMFTLICIISVRTIANYFADFILRWIAGKLSTDLQTLLLEKAL
                     ALPVYHFEQHDLKQFYIDHIANILKIADLSTRVFWGQVKEILIFTGLVAIMFYINRDA
                     AVLGIFLILVTVLIAQILNKGPNHRNSQSLAITKSSDLFQQIMKHIRSIKIDESLRQE
                     RNNFYQRLERQRISLLKPIMGNFLGKLLAIIVFSLMLIAVFYYLLHQYHLHKLVLSEM
                     IAMIGAFGLLAPASARFLNRIAEMDTGRIALQQLNEFLSLQTQPPKEGIALGTIQGAL
                     VIENLKPQGRDSLNDSSSHSFLTIQPKDVVALTAANPGVLKSMLDCVLRFNQPHSGRI
                     MLDGIDIATLKSEEIGTKVAWISPDMPLLEDTLAANIAYGTMHCATEAQLTAIARVSH
                     VADFAREMPYGLQTRINKDGLTSSEALKQCVLLARALLKNPAIVIIDESFVEFELSNE
                     KVDHALDVLMQGRTSIIISTRQPMIQKAQKMVCIDDLMQMTSK"
     gene            5568..6491
                     /gene="rfbA"
                     /locus_tag="PKZ49_07715"
     CDS             5568..6491
                     /gene="rfbA"
                     /locus_tag="PKZ49_07715"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HND36486.1"
                     /translation="MNTHPSEPTQQRPSTPRKGIILAGGSGTRLYPVTQVISKQLLPI
                     FDKPMVYYPLSTLMLAGIRDILIISTPQDTPLFKQLLENGEPWGLNLSYAVQPSPDGL
                     AQAFIIGESFLGNDYSALILGDNIFYGHDFHHLLDNATQRATGATVFAYHVHDPERYG
                     VVGFDSAGRAINLEEKPQQPQSNYAVTGLYFYDQQVVEYAKSLKPSARNELEITDLNR
                     IYLEQNKLHVEIMGRGYAWLDTGTYESLLDASKFVATIEHRQGLKIACPEEIAFRKGW
                     ITAEQLDKLARPLAKNSYGKYLQQILNQPLL"
     gene            6519..7064
                     /gene="rfbC"
                     /locus_tag="PKZ49_07720"
     CDS             6519..7064
                     /gene="rfbC"
                     /locus_tag="PKZ49_07720"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HND36487.1"
                     /translation="MEAIQLAIPDVYLIQPRVFGDERGFFFESYNQQQFNAAINRPIS
                     FVQANHSRSARHVLRGLHYQIKQPQGKLVRVVSGEVFDVAVDLRKHSATFGQWVGEIL
                     SAENKKQLWIPEGFAHGFLVLSESADFLYQTTDYWAPAFERCILWNDPVLNIQWPIDQ
                     SPVLSGKDAQGTSFKLAETYP"
     gene            7109..8458
                     /gene="radA"
                     /locus_tag="PKZ49_07725"
     CDS             7109..8458
                     /gene="radA"
                     /locus_tag="PKZ49_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HND36488.1"
                     /translation="MAKSKSLYFCNACGGQSLKWQGQCPHCREWNTLVEGVAEKKGPS
                     LISGLRPTEIQSLSDIEVTETPRLSTTIGEFDRVLGGGLVLGGVVLLGGDPGIGKSTL
                     LLQALSAMSMDHRVLYVSGEESAQQIALRARRLNLDVSKVSLLTEIHLENIHAMLSNA
                     APAITVIDSIQTIYSDTLQSAPGSVAQVRECTAQLTRIAKSKGICMILIGHVTKEGSL
                     AGPRVLEHMVDTVMYFEGDAHSSFRLIRSFKNRFGAVNELGVFAMTEKGLRGVNNPSA
                     FFLSHHHKPVPGSCVMVTQEGTRPLLVEIQALVDDTHTANPKRLSIGLEQNRLAMLLA
                     VLHRHAGIPCFNHDVFINAVGGIRITEPGADLAVLAAIVSSIKDKPLAEKIVVFGEIG
                     LAGEIRPVQRGQERLKEAAKLGFIKAIIPESNKPKQPIPDIEILTVNRVEQALALLR"
     gene            8455..9270
                     /locus_tag="PKZ49_07730"
     CDS             8455..9270
                     /locus_tag="PKZ49_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phytoceramidase"
                     /protein_id="HND36489.1"
                     /translation="MKSVTTSRKIRIYLLILLTCALLLAAAVLPPVSQSTLYHEFADQ
                     RSYWGIPNFLNVVSNLIFLIVGIAGLIYLRRESLLQQNQPLSAEIKSYRLVFASTAAA
                     CLGSIYYHWEPSLISLFWDRLPIAIGFMSLLAATVMERFATNWVTTYSVILLPIAGAM
                     STLYWLWSELYSVGNLNFYLAAQFCSIILILLLVSSFPSRYSHASEIYRILSIYGLAK
                     LAEIADQEVYQFGNIISGHTLKHILAGLAVYFIMRMLKIRTLKPNHEIYVSKK"
     gene            complement(9320..>9763)
                     /locus_tag="PKZ49_07735"
     CDS             complement(9320..>9763)
                     /locus_tag="PKZ49_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HND36490.1"
                     /translation="HRIGRTGRAGASGVAVSFATGRDSISLRKIERFTGQTIAPFVVP
                     GMESKYKPKTGGGRSHSGRDQRMPNITHKRNRSWGGGNQLTEERKTQNHSNQSKRDNH
                     TFNDFSAGSEARKRPVRNRSGFSSGFNSFSGSGRSNQSRTPRFGS"
BASE COUNT         2765 a         2255 c         1934 g         2709 t
ORIGIN      
        1 tgagcaagtt atcgcagatc tttttgcaca aagcgtgcac aaaagagatt atttacagct
       61 cacgccatca gaacgccagg cgctactctt agatgaaatc acgagcccca ggacactcta
      121 ttcgccttat tttaagtatt cagctacaac acaaagcgag ctaagaattt taaaaatggc
      181 ggccgaaatt cagcatcggt ttggtcatgc agcactaccc aattatatta tttccatgac
      241 caccagcgtt gccaatatct tggaagtagt tttactcctc aaagaagttg gtttgctcca
      301 ttctggagag aacccgagac tcggtttaaa tatcatcccc ttgtttgaga caattggtga
      361 tctaaggggt tgtggcgcaa tgatggatga actcttttca gttccttatt accgtaaatt
      421 gctagaatca cgcggtaacg agcaagaagt catgctgggc tactctgaca gcaataaaga
      481 cggcggcttc cttacctcta actgggaaat ctacaaagct gaaatcaatt taactgaagt
      541 ctttgctaag catcaaatcg aattacgatt attccatggt cggggaggca ccgttggccg
      601 aggcgggggg ccaagctatc agggcattat ggctcaacct cctcatagtg ttaatgggca
      661 aatccgtgtg acggaacaag gtgaagttgt cgccagcaaa tataccgatc ccgaagttgg
      721 gcgtcgcaat ctagaaacac tggtcgctgc aacgatagaa gcgacattat taagccatga
      781 aacgatccag gctgatgtga cgcgttatca tcaagcaatg gaaacccttt cttcgcatgc
      841 gttttcagct tatcgaaatc tcgtatatga aacaccagga tttaagcaat ttttcttgga
      901 atcaacaccc gttcgtgaaa ttgcaggcct tcatatcgga agccggcccg cttctagaaa
      961 gccatcggat cgtattgaag atttacgtgc cattccatgg gtttttagct ggagcttgaa
     1021 tcgaaccatt attccaggtt ggtatggatt tggctctgcc gttgaaaaat tcattcaaca
     1081 acatgagcaa gatgaaactg ggttaaaact gctacaggaa atgtacgcta aatggccatt
     1141 gatgcaaaca ttgctgtcaa acatggatat ggtacttgcc aaaactgatc tgggtattgc
     1201 atcgcgctat gcggaattag ttaccgatgt tagcttgcgt gagcaaatct ttagtcgcat
     1261 ccaagaagag tggcaacgta gtgtcaaatg gcttttccaa atcacgggtc atagcgaatt
     1321 actcgaagat aaccccacgc tcgctcgcag tatccaaatt aggacaccgt acatcgatcc
     1381 gctcaatcac ctacaaattg aattgttgcg tcgctatcgt gcgggtggtg atgttgaaga
     1441 acaagtccat cgctccattc accttaccat caannnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnntcttctt agaaaggata agtagataca aacaccagat catccgccac
     1621 gatcggcgtc ccgattacat taatcaccga atcgatatca gcacttccat tgccttcaat
     1681 atcgacttcg atacgctgtg ttcccaaccc tagatcataa gttagccgta cttcactcac
     1741 accactggca gtaaatggag cattgcctag aaataccacg ctattcggtg accattctgt
     1801 cgattgcaaa attattgtat cttctaaacc attaaaatct gtaatgagat ctggcgcgat
     1861 cgggtttgaa tcacttccag tcccgctact gcttatagta aatataaacg tatcgctatc
     1921 actcccaccg gttaggctat ctgcaccgcg gttaggcctg agcaaatccg cccccgaacc
     1981 accatccaag acatcgtttc ctgttccacc acgaatttta tcgtcaccat taccgccata
     2041 aagactatct cctaggagat caccggcacc actgaatatt gcatcattac catcgccacc
     2101 attaatgata tcgctaccca tcccagcatt aagccaatta tctaggttag agccggtaat
     2161 cgtatcattg aaatcgctac cctggacttg ttctacatta agaatgaggt caatacctgc
     2221 tccaccggta ttttgcgacg taatcaaact caaatcgact gtcacaccac tgatcaaacc
     2281 ctggcgctga taattaatcg tatcgatacc atctccacca tcaatactat cgtttccaat
     2341 aatccccgta tctggaatca agagatcatt tcccaaacca ccatagagtt tgtcattccc
     2401 agcgccacca tcaatctgat cgtgcccatc taacgcataa atcgtgtcac ccccatttcc
     2461 acctaccaga acgtttctag tatcgttacc agtcagcgta tctacaaaat tggtgccatt
     2521 gatattctcg atattaagaa taatctcttt ccctgcaccc ttggtatctt gtaagactat
     2581 cttacgcaaa tcaaccgtta caccgctcac cgcagctgaa acactataat ccagagtatc
     2641 aatgccatct cctccgtcga tcaaatcgct cccaattgca cccgtatcgg gtctgatcaa
     2701 gtcatcacca gccccgccat acaatttgtc attaccggcg cctccatcga gtaaatcatt
     2761 cccatctaaa ccaaaaatcg tatcgcgacc gctaccgccc aataatgtat cattcccact
     2821 taggcctgta atcgtgtcat caccctgcaa gccatcgatc atgtcaccaa gcaaatattg
     2881 cgcatcaatc gtgtcgtttt tgcgcgtacc ataggtaaaa gttgttgcct tctcattaat
     2941 tgccatttgc attctccttt atcatcagaa ttatagttat tgttgatact tacatttttg
     3001 attgtaatcc tttaatactc ttcgatattg atccaaatca atatccaagc atcctccact
     3061 aatagttgga caagcacgaa catagggaca agaaagcaag ggcaatataa taataattac
     3121 cccttcaaac taagtggaac acctaatttg aagaggtttc gccctgataa tattagggca
     3181 ggaggaaaat aaatggagcg tacttatttc ttaagactat caatacaggt tcggactttt
     3241 acatcatcgc ctgcatagat tttttggcaa tcctcaggcg tttgaggcgg gtttgcgaag
     3301 gcagacgaga aggccatcgc aaccatcaag gctaaaacat aagtggtaat tcgttttttt
     3361 atcatcatga ttaaactcct atttaacaat gagtaaagaa aactgacgaa tacgccagta
     3421 attcttattt agcaatgagt gtgccaacct gataaaaatt cattagaaca acaagttacc
     3481 aaaaccgctc atcgataatc cgatcaatcg ctgcacattt tcacgtaatg atgtcgtttt
     3541 gaaccgactt atagatcgac agaataagct atcgcaacga ttagacttga aatagcgctg
     3601 tctttaattt gaaacaaata cccgtaggcg atcgaatcgt aaaatttgct accaatcact
     3661 gcgatgttat gtgacttatt taagaagctg aggaaattcg gctatttaca aatatttacg
     3721 aaaataagat gtcttacacg cgatgttctc cgtgttcact atcatttaga ggtcatttgc
     3781 atcaaatcgt caatacaaac cattttctga gctttctgga tcataggctg acgggttgaa
     3841 ataatgatcg aggttcgccc ttgcatcaaa acatcgaggg catgatcaac tttttcgtta
     3901 ctgagttcaa attcaacgaa ggactcatca ataatgacaa tcgctggatt tttaagtaat
     3961 gcacgcgcca ataacacgca ttgcttgagt gcctcagagg aagtcaatcc atctttattg
     4021 atgcgagtct gcaagccata gggcatttct cgtgcaaaat cagcaacgtg acttacccgg
     4081 gcgatagcgg ttaattgcgc ttctgttgca caatgcatcg tcccatatgc aatattggct
     4141 gccaacgtat cttcaagcaa cggcatatcg ggactaatcc atgcaacctt tgtacctatc
     4201 tcctcgctct tcagcgtagc aatatcgatt ccatccagca ttatccttcc tgagtgcggt
     4261 tgattaaatc gtagtacaca atccagcatt gatttgagca ccccagggtt tgctgcggtt
     4321 aatgccacca cgtctttggg ttgaattgtc aaaaaactat gggacgatga atcattaagc
     4381 gaatcacgac cttgcggctt aagattctca ataacgagcg cgccttgaat cgttccaagg
     4441 gcgattcctt ctttaggagg ctgtgtctgc aatgatagga attcattgag ctgttgcaac
     4501 gcaatccgtc ctgtatccat ctctgcaata cgattcaaaa accgagctga agcaggcgca
     4561 agtaatccga atgcaccaat cattgcaatc atttcactga gcactagctt atgaagatga
     4621 tattgatgca acaagtaata aaaaactgca atgagcatta gagaaaagac aatgatcgcc
     4681 aggagcttac ctaagaaatt gcccataatc ggtttcagta aggatatccg ctgtctctca
     4741 agtcgttgat aaaaattgtt tctttcttgg cgtaaactct catcaatttt gatcgatcga
     4801 atatgcttca taatttgttg aaataaatcc gacgattttg tgattgccaa ggattgacta
     4861 ttacgatgat tgggtccttt attaaggatc tgagcgatta aaacagtaac caaaatcaaa
     4921 aagattccta gcaccgcggc atcccgattg atataaaaca tgatggcgac taagccagta
     4981 aaaatgagga tttctttaac ttggccccaa aatacgcgcg tactgagatc tgcaattttc
     5041 aatatattcg caatgtgatc tatgtaaaat tgcttgaggt catgctgctc aaagtggtaa
     5101 accggtaatg ctagcgcttt ttctaataga agcgtttgaa gatcggtcga tagttttccc
     5161 gcaatccagc gcaagataaa atctgcaaag taatttgcta ttgttcgcac gctgataata
     5221 caaatcagcg taaacatcgt taccatcatt gtttcttgat cttggtctag catgtgatcc
     5281 agtaacgcct tggccactaa aggcagtgtt gcggatgaca cgccaacaat caaaagaatc
     5341 gctgcgacaa gccaaaagaa aactcgatag ggtttacacc aattaatcag caaatggatc
     5401 gattttgttt cactcatggt gcatcatcat caatgctaaa attattcaaa ataaatgcaa
     5461 tggctgcgta cataaagtca tctcttttta gtaaactcac agcatattca ggattcagtc
     5521 aatttatcaa tccgtttagg aaaacttaat ttcattcgat caggacaatg aatacacatc
     5581 catccgaacc tacccaacag cgaccttcca cacctagaaa aggtatcatt ctagcaggtg
     5641 gttcaggtac tcgactctac cctgtaactc aagtcatttc aaaacaactc ttaccaattt
     5701 ttgataagcc aatggtttat tatccgctga gcaccctaat gcttgcaggg attcgcgata
     5761 ttcttatcat ctcaacaccg caagataccc cgctcttcaa gcaattattg gagaacggtg
     5821 aaccctgggg gctcaatttg tcctatgccg tgcaaccttc ccccgatggc ttagctcaag
     5881 cctttatcat cggtgaatcg ttcttgggaa acgactattc tgcattaatc ttaggtgaca
     5941 acatttttta tggtcacgat ttccaccatt tactcgataa cgccacgcaa cgcgcaacgg
     6001 gtgcaacggt ttttgcgtac catgttcatg accctgaacg ctatggcgtg gttggattcg
     6061 attctgccgg ccgagcgatc aatcttgaag aaaaaccaca gcaaccccaa tcaaactacg
     6121 cagtaacggg cttatatttt tatgatcagc aggttgttga atacgcaaaa agtttaaagc
     6181 cttccgcgcg aaatgaattg gaaattaccg acctcaatcg aatttatcta gaacaaaaca
     6241 aactccacgt ggaaattatg ggcagaggct atgcttggtt ggataccggc acatacgaat
     6301 ccttactcga tgcgagcaaa tttgtagcaa ccatagaaca tcgccaggga ttgaaaatcg
     6361 catgtcctga agaaatcgca tttcgtaaag gatggattac tgcagagcag ttagataaac
     6421 tggctcgccc tttagctaag aatagctacg gtaagtattt acagcaaatt ttgaaccaac
     6481 ccttattatg atcgcttgag aaaattaagg aacaaaaaat ggaagccatt caattagcca
     6541 ttccagatgt ttatttgatt caaccgcgtg tatttggcga tgaacgaggc ttcttttttg
     6601 agagctataa tcagcaacag tttaatgcag cgatcaatcg acccattagc ttcgttcaag
     6661 ctaaccattc gcgttcagcg cgccatgtgc tgcgtggctt acattatcaa atcaaacaac
     6721 cacaaggtaa gttggttcga gtcgtcagcg gagaagtttt tgatgtggct gtcgatcttc
     6781 gcaagcactc tgctaccttt gggcaatggg ttggtgaaat tttaagcgct gaaaataaaa
     6841 agcagctctg gattcctgaa ggctttgcac atggctttct cgtgctttcc gaatcggccg
     6901 acttccttta ccaaacaacc gactattggg caccggcgtt tgaacggtgc atcctctgga
     6961 acgatcccgt attgaatatt caatggccaa tcgatcagtc tcctgtgcta tcgggtaaag
     7021 atgcacaagg cacatcgttt aagctcgccg aaacttaccc ttgaacacta cgttcccatt
     7081 agcaccaacc gccattaatt actaatatat ggcaaagtcc aaatcgctat acttctgcaa
     7141 tgcgtgcggc gggcaatcct tgaaatggca gggacagtgc cctcactgtc gcgaatggaa
     7201 taccttggtt gaaggcgttg ctgaaaaaaa agggccaagt ttgatttcag gactaagacc
     7261 aactgaaata caaagcttga gcgatatcga agtcactgaa acaccccgtt tatcaacaac
     7321 aatcggcgaa ttcgatcgtg tactgggggg tggtttggtg ctggggggag tcgtgttatt
     7381 agggggagac ccgggcatcg gcaaatcaac actgctgctt caggcgcttt cagcaatgtc
     7441 gatggatcat cgcgtattat atgttagcgg cgaagaatcg gcgcagcaaa tcgctttaag
     7501 agcacggcga ttaaatctcg atgtcagtaa agtcagcttg ttgactgaga ttcatcttga
     7561 aaatatccat gcaatgctta gtaacgcagc acctgcgata acagtcatcg attcaattca
     7621 aacaatctat tcagatacct tgcaatcagc ccccggttct gtggcacaag tcagagagtg
     7681 tactgcgcaa ttgacgcgaa ttgctaaaag taaaggaatt tgtatgattc tgatcggtca
     7741 tgtgacgaaa gaaggttcgc ttgccgggcc cagagtgctt gagcatatgg tcgataccgt
     7801 tatgtatttc gagggtgatg ctcactctag tttccgtttg attcgctcat tcaagaatcg
     7861 atttggcgcc gtcaatgagc tcggtgtttt tgcaatgacg gaaaaggggt tgcgcggtgt
     7921 taataatcca tccgcttttt ttctctctca ccaccataag cctgtgccag gttcctgcgt
     7981 tatggtcact caagaaggaa ctcgcccctt attagtcgaa attcaagctt tggttgatga
     8041 cacccacact gccaatccca aacgtttgag tatcggttta gaacaaaacc gactggcaat
     8101 gcttttagct gttctgcatc gccatgccgg aattccttgt ttcaatcatg atgtcttcat
     8161 caatgcagtg ggtggtatcc gaattaccga gcctggcgct gatttagcag ttttagccgc
     8221 gattgtttca tcaattaagg ataaacctct tgcagaaaaa atagtggtat ttggcgagat
     8281 tggcttagct ggagaaattc gccccgtaca acgcggccaa gagcggctaa aagaagcagc
     8341 aaagctcggg ttcatcaaag ccatcatccc cgaatcaaat aagcccaagc aacccatccc
     8401 tgacatcgaa atccttaccg taaaccgagt ggaacaagca ttagcgctac tgcgatgaaa
     8461 agcgtcacta catccagaaa aattcgtatc tatttgttaa ttttacttac ttgtgcattg
     8521 cttctggctg ccgctgtgct accaccggtc tcacaatcca ccttatatca tgagttcgct
     8581 gatcaacgct cgtattgggg tattcctaat ttcctcaacg tggtttctaa tctaattttc
     8641 ttgattgttg gcatcgcggg tctaatttat ttgaggaggg aatcattatt acagcaaaat
     8701 caaccgttat ccgctgaaat aaaatcatac cgactggtat ttgctagtac cgctgctgct
     8761 tgcttgggtt cgatttacta tcactgggaa cctagcctga ttagcctatt ctgggatcga
     8821 ttacccattg caatcggatt tatgagctta cttgccgcga ctgtaatgga acgctttgct
     8881 actaactggg taacaaccta ttctgtaata ttattaccta ttgctggcgc aatgagcaca
     8941 ctatattggc tctggagcga gctttatagc gttggtaact taaatttcta tctggctgcg
     9001 caattttgtt caattattct cattctcctg ctggttagca gttttccatc tcgctatagc
     9061 catgcgtcgg agatttatcg catcctgtcc atttatggtt tagctaaatt agctgagata
     9121 gctgatcaag aagtctatca attcggaaac atcatcagcg gacataccct taaacacatc
     9181 ctcgctggac ttgcagttta tttcattatg cgcatgctaa aaatacgaac actgaaacca
     9241 aaccatgaaa tatacgtcag taaaaaataa aaaccccttt agcaaaattt gctaaagggg
     9301 tggtaatttt agaagttact caagaaccaa aacgcggtgt acgcgactga ttacttcttc
     9361 ctgaacctga aaatgaatta aatccagatg aaaatccact gcgatttcgg acaggtcttt
     9421 ttctagcctc agagccagct gaaaaatcat taaacgtatg attatcccgc tttgattgat
     9481 tggagtggtt ctgcgttttt ctttcttcag ttaattgatt acctcccccc cacgaccgat
     9541 ttcgtttatg cgtgatattt ggcattcttt ggtctctacc gctgtgtgaa cgcccaccac
     9601 cagtctttgg tttatatttt gactccattc ctggaacaac gaaaggggca atcgtttgcc
     9661 ctgtaaatcg ctctattttt ctcaggctga tgctgtccct tccagttgca aatgatactg
     9721 ctacgcctga tgctccagct cgtccggttc gaccaatccg atg
//
LOCUS       DAOQKQ010000100         9591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_3378_length_9591_cov_6.380348, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000100 DAOQKQ010000000
VERSION     DAOQKQ010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 9591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9591
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3378_length_9591_cov_6.380348"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1459
                     /locus_tag="PKZ49_07740"
     CDS             <1..1459
                     /locus_tag="PKZ49_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HND36491.1"
                     /translation="FYPAWGRARLEAMIKNRPDWCISRQRNWGVPMTFFVHKETQAVH
                     PRTLELLEAVAKAVEQQGIEAWFSLDPATLLGNEADQYQKLTDTLDVWFDSGTTHDTV
                     LRRNIHLNHPADLYLEGSDQHRGWFQSSLLTGCAMNHRAPYDALLTHGFVVDGQGHKM
                     SKSKGNVIAPQKIVDTLGADILRLWVASTDYSGELFISDEILKRIVETYRRIRNTLRF
                     LLANIADFNPQTDQLPVEQWLEIDRYMLAHTEALQNELTALYQRYEFHQSVHALHNFC
                     SEDLGGFYLDILKDRLYTTGSKNLPRRSAQNALYHITQSLVRLFAPILSFTAEEVWEQ
                     LTNKPEDSVLLQSWHSFPVQARDQIDFLLARWSRLRQLRSQVLKKLEESRAQGAIGSS
                     LAAVVTIQAAGEDFTLLQSLGHDLRFVMITSEVHLHHIAHTNDIMITVSASSHQKCER
                     CWHYREDVGHHADHPTLCQRCISNLYGAGEHRKHA"
     gene            1490..1954
                     /gene="lspA"
                     /locus_tag="PKZ49_07745"
     CDS             1490..1954
                     /gene="lspA"
                     /locus_tag="PKZ49_07745"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HND36492.1"
                     /translation="MKLYLSLIIAFIVVALDLVTKYWVESALVFGQQIPITSFFNLVL
                     TYNPGAAFSFLGDASGWQRWFLSGIAFIACAVISYLLYKHTENKLFCWALSLVLGGAL
                     GNLYDRITLGHVVDFLDFYIGDYHWPAFNVADSAICIGAALLIYESFTTKKP"
     gene            2031..2348
                     /locus_tag="PKZ49_07750"
     CDS             2031..2348
                     /locus_tag="PKZ49_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HND36493.1"
                     /translation="MKKAYSANNLIEAQMMLQLLEQAYIPAKLMNTFSQGGLGEIPFT
                     HAYPEVWVIRDDDFEKAQNIIDQYEHTSIDPGHVNCPNCSESNPANFELCWNCGHGLE
                     NKG"
     gene            2552..4615
                     /locus_tag="PKZ49_07755"
     CDS             2552..4615
                     /locus_tag="PKZ49_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HND36494.1"
                     /translation="MFLQNLASAGSFLVMLTIISCGFANIATASNTSTPAGKASMDPL
                     MDLSIEELMQIKITSVSRREQKLSGTPSAIFVITQEDIRRSGATSIPEALRMAPGVEV
                     ARVGTDKWSISVRGFNGRFANKLQVLMDGRSVYTPLFSGVIWSQQDTLMEDIERIEVI
                     RGPGATVWGANAVNGVINIITKKAIDTQGTMITAGGGSFEQGFIGMRYGGKINEATPF
                     RIYAKGFSRDNTHSLAGSNLQDNWRSARAGFRLDHTRGIDQLTLQGDVFINRIGDSLL
                     QPTLISPFSRLDVVDTTEKGGNIRFRWDRNFSDQSSIMLQTYYDRVQNQLTPWTKYAE
                     SFDIDFQHRLPILKSHDVTWGLNYRLYHNKVAETELTQFNPAQKTNHNFSAFIRDEVT
                     VIPEKLSLSIGTRLDHNDFTGLEIQPSGRLMLTPNEYHSIWLSVSRAVRIPSRGENDV
                     TIAGRTLQSLPGLPGLPIPVLMAVQGSNQFASEKLIAYEMGYRHQVSANASIDLATFF
                     NDYSQLRDFRFGQMMPGTGVIPHWILPVIFSNTTSAYSYGFESAIDWRPFKKWRLQSS
                     YSYLTIHTNSNPEFRQLDSSSAGADKANPNHQLSIRSHYDFSEQLQLNLWLRYVSALT
                     FFNIPSYVTMDAKLSWKPVRNTELFVVGQNLFSKNHRESQSDFIPSIPALIPRGIYVG
                     IERRF"
     gene            4711..5112
                     /locus_tag="PKZ49_07760"
     CDS             4711..5112
                     /locus_tag="PKZ49_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36495.1"
                     /translation="MKLDSTIIRWILLVPATWVAWYIAVFLGIAILTAGDAFCPSEQI
                     VSGWCHANWYPFFLQSVMVFSSSVSAIFVVVAGYYMAPSHRSFVAKLIFITGSAVAIF
                     MAIETQEYLALTGALVCGLGSLLIVIKDRAQ"
     gene            complement(5594..5857)
                     /locus_tag="PKZ49_07765"
     CDS             complement(5594..5857)
                     /locus_tag="PKZ49_07765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HND36496.1"
                     /translation="MNYHIQFTPQALEDLSQLDNVVAKRIVDKIQWLAEHYDRIQPET
                     LTGTFANFYKLRVGNWRILYTCDMTHCHITIHVIAHRSKVYKS"
     gene            complement(5854..6054)
                     /locus_tag="PKZ49_07770"
     CDS             complement(5854..6054)
                     /locus_tag="PKZ49_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36497.1"
                     /translation="MKAADLTVDELQALIRKVVHEELQNIMADPDQHLELTDEIKTRL
                     ELSLGSSERISLQEVKDRLKLA"
     gene            complement(6536..6625)
                     /locus_tag="PKZ49_07775"
     tRNA            complement(6536..6625)
                     /locus_tag="PKZ49_07775"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6589..6591),aa:Leu,seq:taa)
     gene            7017..8255
                     /locus_tag="PKZ49_07780"
     CDS             7017..8255
                     /locus_tag="PKZ49_07780"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HND36498.1"
                     /translation="MQSVGLNARNASRAVAKADTATKNRALQAMAEAIRRDERKLIDA
                     NQQDLDTARSKELSSAMIDRLTLSTKGIQSMAEGLLQIAALADPIGEIKDLNFRPSGI
                     QVGRMRVPLGVIGIIYEARPNVTADAAGLCLKAGNAAILRGGSEAIHSNQAIAACVKE
                     GLIVAGLPEAAIQVIETRDREAVGELITMKAFVDVIVPRGGKGLIERIANEAQIPVIK
                     HLDGICHVYIDDQANIDKAIRVADNAKTQRYGTCNTMETLLVHAGIAQQILPALCKIY
                     FDKGVELRGNQAACAIVSPMKLATEVDWGTEYLAPILNVRVVDSLDQAIDHITTYGSQ
                     HTDSIITENFTHARRFLREVDSSSVMVNASTRFADGFEYGLGAEIGISTDKIHARGPV
                     GLEGLTSQKFIVLGDGHIRQ"
     gene            8309..>9591
                     /locus_tag="PKZ49_07785"
     CDS             8309..>9591
                     /locus_tag="PKZ49_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HND36499.1"
                     /translation="MANTLTITIPDIGDFKDVPVIEVLVKPGDTVEKETPLITLETDK
                     AAMEVPSPQAGIVKTLHIKVGDKVSQGSAILTLELSAVAESSSESVAASSQSAQTIQP
                     DTQSTAKMSGDMHAEVVVLGAGPGGYTAAFRSADLGKKVILIERYSSLGGVCLNVGCI
                     PSKALLHVAKVIAEAKAASKHGIDFGKPNIDLDQLRTWKESVINKLTKGLSALAKQRK
                     VQVFQGIGRFLNPHALEVATPDGKKIVTFDHCIIAAGSTATRIPGFPDDPRLMDSTDA
                     LALTEIPKRMLIVGGGIIGLEMASVYHALGSQITIVELMDQLIPGADADLIKPLHKQL
                     MNRYEAIYLQTKVSKITTEKKTLKVTFEGAQAPAEQSYDRVLVAVGRRPNGKTINAEL
                     ANIQVNERGFIPVDSQMRTNVPHIFAIGDIAGDPML"
BASE COUNT         2643 a         2040 c         2242 g         2666 t
ORIGIN      
        1 attttaccca gcctggggaa gagcacgctt agaagcgatg atcaagaatc gccccgattg
       61 gtgcatttca cgtcagcgaa attggggtgt gccgatgact ttctttgtcc ataaggaaac
      121 tcaagccgta cacccacgca cccttgagtt actcgaagcc gtcgccaaag ccgtagaaca
      181 gcaaggcatt gaagcttggt ttagcctcga tcctgctacg ttgttaggca atgaagcgga
      241 tcagtatcaa aaattaactg atacgctcga tgtttggttt gattcaggta caacccatga
      301 tacggtgctg agacgtaata ttcacttgaa tcatccagcc gatctctatt tagaaggctc
      361 tgatcaacat cgtggctggt tccaatcgtc acttttaact ggctgtgcaa tgaatcatcg
      421 cgctccttac gatgcgttac tcacccatgg ttttgtggtt gacggtcaag gccacaaaat
      481 gagcaaatcc aagggtaatg tcattgcacc ccaaaaaatt gtcgacacgc taggcgctga
      541 tatcctgcgt ttatgggtcg cttctaccga ttattcgggt gagttgttta tctctgatga
      601 gattttaaaa cggatcgtcg aaacctatcg ccggattcgt aataccctac gttttttact
      661 cgctaacatc gcagatttca atcctcaaac cgatcagcta ccggttgaac aatggttgga
      721 aatcgatcgt tatatgctcg cgcataccga agcgttgcaa aatgagctga cggcgctcta
      781 tcaacgttat gaatttcatc aaagcgtgca tgctttacat aacttctgct cggaggactt
      841 aggcggtttt tacttggata ttttgaaaga ccgtttatac actaccggtt cgaagaatct
      901 tccgcggcgt tcggcgcaaa atgcgcttta ccatatcaca cagagtctgg tacggttgtt
      961 tgcaccgata ttgagcttta ctgccgaaga agtctgggaa caattgacaa ataagcctga
     1021 ggatagtgtg ttgctacagt cgtggcattc atttcctgtg caagcacgtg atcaaatcga
     1081 ttttttattg gcacgctgga gccgcttgcg tcaattgcgt tcacaagtat taaaaaaatt
     1141 agaggaatcg cgcgcgcagg gagcaatcgg ttcctcgctt gcggccgttg tcacgattca
     1201 agcagcggga gaagatttta cgctattaca atcgctaggc cacgatctgc gttttgtcat
     1261 gatcacgtct gaagttcatt tacatcacat agcgcatacc aacgatatca tgattacagt
     1321 ttcagcaagc tcgcatcaaa aatgtgagcg ctgttggcat tatcgtgaag atgtcggtca
     1381 tcatgccgat catccaacgc tctgtcagcg ttgtatctcc aatttatatg gcgctggtga
     1441 acatcgtaaa catgcctaaa tttcttgtat tgattaccta ctcattatca tgaaactcta
     1501 tcttagtctg atcatcgctt tcattgttgt agcactcgat ttggttacaa agtattgggt
     1561 agagtcggcg ttggtttttg ggcaacaaat tcccatcact tcctttttta atttggtgct
     1621 gacatataat ccaggcgctg catttagctt cctgggcgat gcctccggct ggcaacggtg
     1681 gtttttaagt ggcatcgctt ttatcgcatg cgcagtcatc agttatttac tttataaaca
     1741 cactgaaaat aaattgtttt gctgggcgtt gagtttggta ctcggtggtg cgctaggaaa
     1801 tttatatgat cgcattacat taggccatgt cgttgatttt ctcgatttct atattggcga
     1861 ctatcattgg cctgctttca acgtggctga ctcggcaatt tgtattggcg cagcattgct
     1921 aatctatgaa agttttacaa ccaagaaacc ctaaatgaat cgtgtattaa ttgcggtacc
     1981 gctgtgatgt attcaggaaa ttgaatcgca tgtagccata actctctacg atgaaaaaag
     2041 cgtattcagc taataatctg atcgaagccc agatgatgct tcaattatta gagcaagcct
     2101 atattccggc caaactgatg aatactttct cacaaggcgg tttaggagaa attcccttta
     2161 cccatgctta ccctgaagtt tgggtgatac gcgatgatga ttttgagaag gcgcagaaca
     2221 tcattgatca atatgagcac acttcgattg accccggaca cgtaaactgc cccaattgta
     2281 gtgaatccaa tccagccaat tttgaattat gttggaattg tggtcatggg ctagaaaata
     2341 agggttaaat gaagaccacg ttctacctcc aagttttatt gcccaaactg tcctgactgc
     2401 tgattggaac ttcatttcaa aattaacaat cgttagcagt tcatcttgaa ttcttgaaaa
     2461 agaaggagca aaaccaacga aattggaatt tttatcatgg caacttaaga taaaatttaa
     2521 catcccaaat caataaatat ccgattaacg catgtttttg caaaacctgg cttcagcagg
     2581 cagtttctta gttatgctga ctattatttc ttgcggtttt gcaaatatcg ctacagcttc
     2641 aaatacttca acaccagctg gcaaagcttc gatggatcca ctgatggatt taagtatcga
     2701 agaattgatg caaattaaaa ttacatcggt gtcccggcgc gaacaaaaat tatctggcac
     2761 gccctccgca atttttgtta tcacacaaga ggatatccgc cgctctggcg cgactagtat
     2821 tccagaagca ctgcgtatgg ccccgggtgt cgaagttgcc cgggtgggca cggataagtg
     2881 gtcaatcagt gttcggggct tcaatggtcg atttgcaaat aaactacaag ttttaatgga
     2941 cgggcgtagt gtttatacac cgttattttc aggtgtaatc tggagtcaac aagacaccct
     3001 catggaggat attgagcgaa tcgaagtgat tcgtggtccg ggcgcaaccg tttggggggc
     3061 taatgctgtt aatggtgtta ttaacatcat cactaaaaag gccattgata cgcaaggcac
     3121 catgattact gcagggggtg gtagctttga gcaggggttt attggcatgc ggtacggagg
     3181 aaaaatcaac gaagcgacgc cgttccggat atacgctaaa ggatttagtc gcgataatac
     3241 gcattcactg gctggaagta atctacagga taactggcgt tcagccaggg ctggatttcg
     3301 cttggaccat acccgcggca tcgatcaatt gacgttgcag ggcgatgttt ttattaatcg
     3361 aattggcgat agtttattac agcctacgtt aatatctccc tttagtcggc ttgatgttgt
     3421 tgatacgaca gaaaaagggg gaaatattcg tttccggtgg gatcgaaatt tttctgatca
     3481 atcatcgatc atgctgcaaa cctattatga tcgcgtccaa aatcaactga cgccatggac
     3541 aaaatatgct gaaagctttg atatcgattt tcaacatcga ttgccgatac taaaaagcca
     3601 cgatgttact tgggggctaa attatcgact gtatcataat aaagtcgcgg aaacggaact
     3661 cacgcaattt aaccctgcac aaaaaaccaa tcataacttt agcgctttta ttcgtgatga
     3721 agtgacggta atcccagaga aactgtcatt gtcaattggt acacgccttg atcacaatga
     3781 ttttaccgga ttagagatac aaccgagtgg ccgtttaatg ttgacgccta acgagtatca
     3841 ctcgatttgg ttatcggtat cgcgtgcagt aagaatcccg tcacgcggtg aaaatgatgt
     3901 aaccatcgct ggccggacat tacaatcgtt gccaggattg cctggattac ccattccagt
     3961 actcatggca gtacaaggga gtaatcagtt cgcttcagaa aaactaattg cttatgaaat
     4021 gggttatcgt caccaggtgt cagcaaatgc atcgattgat ttggcaacct tttttaatga
     4081 ttatagccaa ttaagggatt ttcgctttgg tcaaatgatg cctggaacag gtgtgatacc
     4141 gcattggata ttaccggtaa tattcagtaa cacgacttca gcctactcgt atggttttga
     4201 atctgcaatt gactggcgcc cgttcaaaaa atggcgctta caaagcagtt actcctattt
     4261 aactattcac acgaattcga atcctgagtt tcgacaacta gactcatcct ctgcgggtgc
     4321 tgataaagcc aacccaaacc atcagctctc gatccgatcg cattacgatt tttcggaaca
     4381 gttgcaatta aatttgtggt tgcgctatgt cagtgctttg actttcttca atattcccag
     4441 ctacgtaacg atggatgcaa aactatcctg gaaacccgtg aggaatacag agctttttgt
     4501 tgtgggccaa aatttgttta gtaagaatca tcgtgaatca caatcagatt tcatcccttc
     4561 tattccagcg ctgattccac ggggtatata tgtgggcatt gaaagacgtt tctaaaaaag
     4621 cgatgaatct aaaattctct atctgctgag ttgtgagtca gatcattttt ctgctgcgcg
     4681 agtggtttgc taattactaa cctaaacata atgaagcttg atagcactat tatccgctgg
     4741 atccttctgg taccagcaac ttgggtagct tggtatattg ctgttttctt aggcatcgca
     4801 atccttacgg ctggtgacgc attttgtccc tctgagcaaa tcgtgtcagg ttggtgccat
     4861 gccaattggt atcccttctt tcttcaatca gtaatggttt tttcttcgtc agtatcagct
     4921 atttttgtcg tcgtggctgg ttattacatg gcaccatcac accgatcttt cgttgcaaag
     4981 ttaattttta tcacaggttc ggcagttgct atcttcatgg cgattgaaac ccaagaatat
     5041 ctggcgctta ccggcgcact cgtatgtgga ttaggctcgc tattgatcgt gataaaggat
     5101 agggcgcagt gagcaacaaa tagttgatgc tgattctcag gcacagcaac ttgctgtcat
     5161 gaatcaaaac tggtgccgga gccggaatcg aaccggcacg gacggataag gtctgaggga
     5221 tcttaagtct acctaaatag caaacaaaat caaaacgtta cctagatctt attccacaaa
     5281 aataggtttt agacgtcgcg aagaacgcct gtgcggtgaa ttcgcaaagc aatccaccat
     5341 tggtgccata gatcgaagga ttgacccgaa actgatcgaa taaccttgat gtggataaat
     5401 tattttctgt attgggttgt tttggtggtg tagttgtgcc gaattatcgg cggaattgga
     5461 agtgggggaa tgcggatagg aatggtcttg atgaaagatt ttgtgttgga gttcgtgtaa
     5521 cacgctccaa cctgccatgt acgataaagt taaggaaaaa taaccaatga ttatgcaagt
     5581 accagaactg aaatcaggat ttgtagactt tgctgcgatg agcaataaca tgaatcgtga
     5641 tatggcaatg cgtcatatca caggtataca ggatacgcca gttaccgact cgaagtttat
     5701 aaaagtttgc gaaagtacct gtcaacgtct cgggctggat tcggtcgtaa tgctccgcga
     5761 gccattggat tttatctact atcctttttg caaccacatt atcgagctga gaaagatcct
     5821 caagtgcttg aggcgtgaac tggatgtgat aattcaagcc agcttcaacc tgtcttttac
     5881 ttcctggagt gagatgcgtt cgctggaacc tagggacaat tcaagccgcg ttttgatttc
     5941 gtcagttagt tcgaggtgct ggtcaggatc cgccatgatg ttctgtaact cttcatgtac
     6001 aactttcctg atcagggctt gcaattcatc tacagtaaga tcggctgctt tcataggatt
     6061 accctcttca atcatttaat taacggaaaa gaattgtgct cattatatag gctaacgaat
     6121 agagaagtgg atcccatttt ttgggcaggt accttaggag ttattttccg acataccaac
     6181 ctgtcttttg cagcctgaca gactgataat aaaactgatt aggtgctgcc atcaaggtct
     6241 ggtgcttccg gttttcatta taaaaccgga aatactttgc caacaaatgt gttgcaacga
     6301 ccggttgaat ccaccgttcc gagctttacg agggaaagca gcaacagata aaactgaaaa
     6361 ttcagggaat gttagagaag ctcaaaaaca actcgatcat tctacgatta gcatgacaga
     6421 aaagtatttg aagaacagga aaggaggcaa agtgacaccg acaaaataat tctattttgt
     6481 gagttattca cagtttttgc ggaaaaaatt ttgctacaaa aaaatcgttc gaaactggtg
     6541 cccggagccg gaatcgaacc ggcacggacg gataaggtcc gagggatttt aagtcccttg
     6601 tgtctaccaa tttcaccacc cgggcagtat ttaaatcaat gctgctttct cagctcagga
     6661 tgagctttga tgaaagcaag taagaaagcg attatacata gaaataatcg ctttagaaac
     6721 agctagcccc ttttgaaaat tcacgattgt ttttttatca acctgaatcg attgctggca
     6781 tgctgaattt cagccctgga gttgtaaggt ttgcaatcgc ggcttgattg cttacgatgc
     6841 tattgttaca aaacgataag tgagtggcgg aaatttactt ttttgcactg aaagtttctt
     6901 ttgggaatat tacgcgagac ggtatcctcc catgtaaaat accaatcatt aaagtaaatg
     6961 agtcaaactt tttctgggtg gagagaaaag tggaagtaac caatattaaa acctatatgc
     7021 agtcggtcgg gctaaatgca cgcaatgctt cccgagccgt agccaaagca gataccgcaa
     7081 ccaagaatcg ggcgctacag gcaatggctg aagcgattcg acgcgatgaa cgaaaattaa
     7141 tcgatgccaa tcagcaggat ctagatacgg cccgatccaa agaattgagt agtgcgatga
     7201 tcgatcgttt aaccctttca accaagggta tacaaagtat ggctgaaggg ctattgcaga
     7261 tagccgcact ggctgatccg attggggaga ttaaggatct aaattttcgt ccttccggca
     7321 tccaagtggg aagaatgcgc gtgccgctgg gggtcattgg tatcatttat gaagcgcgcc
     7381 cgaatgtgac tgccgatgca gcggggttgt gcctcaaggc aggtaatgcg gcaattttgc
     7441 gcggtggctc ggaagccatt catagcaatc aagcgattgc agcttgtgtg aaggaagggc
     7501 tgatcgttgc ggggctgccg gaagcggcga ttcaagtcat cgaaacccgt gatcgcgaag
     7561 cggtaggtga attgatcacg atgaaagcct ttgtggacgt catcgtacca cgaggcggga
     7621 aaggcttgat cgaacgtatt gccaatgaag cacagattcc agtcattaag cacctggacg
     7681 ggatttgcca tgtgtatatc gatgatcaag ccaatattga taaagctatt cgcgttgctg
     7741 ataatgccaa aacacaacgc tatggcacct gtaataccat ggaaacctta ttggtgcatg
     7801 caggcattgc acagcaaatt ttaccggcgc tctgtaaaat ttatttcgat aaaggcgtgg
     7861 aattacgtgg gaatcaagcc gcatgtgcga ttgtgtcgcc catgaaatta gccactgaag
     7921 tagactgggg tactgagtat ctggccccga ttctcaatgt gcgggtggtt gatagcctgg
     7981 atcaagccat cgaccatatt acgacgtatg gttctcagca taccgattcc atcatcacgg
     8041 aaaattttac ccatgcgcgt cgctttctgc gcgaagtgga ttctagctcg gtcatggtca
     8101 acgcatccac gcgctttgca gatggtttcg agtatggcct gggagcggaa atcggtattt
     8161 cgacggataa aattcatgcg cgcggtccag tgggattaga aggcctgacc agccaaaaat
     8221 ttatcgtatt gggggatgga catatccgcc aataaaggct gcgatgcgtt cactgttttc
     8281 gattgtttag attgatataa aaaactgcat ggcaaatacg ctcacgataa ccattcctga
     8341 catcggcgat ttcaaagatg ttccagtgat cgaagtgttg gtcaaaccgg gtgatacggt
     8401 agaaaaagaa actccgctga tcaccctgga aaccgacaaa gccgcgatgg aagtaccttc
     8461 accccaggca ggcatcgtca aaaccttgca tatcaaagtg ggcgataaag tatcgcaagg
     8521 gagtgctatt ttaacgctgg agctatcggc tgtcgctgag tcctcaagtg aatctgtggc
     8581 tgcatccagt caatcagccc aaaccatcca gcccgatacg caatcaaccg cgaaaatgtc
     8641 aggcgatatg catgccgaag tagtagtgct cggagcaggg ccgggtggat atacggctgc
     8701 tttccgttcc gcagatctcg gtaaaaaagt aatcttgatc gaacgttatt caagcttagg
     8761 gggcgtttgt ctcaatgtcg gttgcattcc atccaaagcc ttgctgcatg tcgccaaagt
     8821 gattgcagaa gcgaaagcag cctcgaaaca tggcatcgat tttggtaaac cgaatattga
     8881 tctcgaccaa ttgcgcactt ggaaagaatc ggtcatcaat aaattgacca aaggcctatc
     8941 tgcattggcc aaacagcgca aagtgcaggt atttcagggc ataggtcgat ttctcaatcc
     9001 ccatgcgctg gaagttgcta cgcccgatgg gaagaaaatc gttacctttg atcattgcat
     9061 cattgctgct ggttcaacag caactcgtat tcctgggttt cctgatgatc cgcgattgat
     9121 ggattcaacc gatgccttgg cattaaccga aattcctaaa cgcatgctga ttgtcggcgg
     9181 gggaatcatc ggtcttgaaa tggccagtgt ctatcatgcg ctggggagtc aaatcacgat
     9241 cgtggaactc atggatcaac tgataccggg tgctgatgcg gatttgatca aaccactgca
     9301 taaacaattg atgaatcgtt acgaagccat ttatttacaa accaaggtca gtaaaattac
     9361 caccgagaaa aaaacactga aagtgacttt tgaaggagct caagcgccag ctgagcaaag
     9421 ttatgatcgg gttttggttg cggtcgggcg ccgccccaat ggaaaaacga ttaatgccga
     9481 gctagccaat attcaggtca atgagcgtgg ttttattccc gtggattcgc aaatgcgcac
     9541 taacgtaccg catatttttg ccatcggtga tattgcgggt gacccaatgc t
//
LOCUS       DAOQKQ010000101         9478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_3429_length_9478_cov_5.411546, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000101 DAOQKQ010000000
VERSION     DAOQKQ010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 9478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9478
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3429_length_9478_cov_5.411546"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1761
                     /locus_tag="PKZ49_07790"
     CDS             <1..1761
                     /locus_tag="PKZ49_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HND36500.1"
                     /translation="PRIQVLENGIGSLDVSSLSPDHPVAIETLNASQIEILRGPATLL
                     YGGGATGGVVNTVNRNIPNQLHKTLTGSFEARGNTATEERAGSVNLSRSFGSMSWNVS
                     GFRRNTGDYNIPGQANISGISPEAEGGSSAHSINNRTHELKNSAAMSQGGSVGGSYVG
                     ERGFLGGSISLMNSKFGIPSLEAPTIDMTQARYNLAGALDNPIKGFEKLKVRTGYNDY
                     KHDERERTGELGTRFKNRELETRVELLHTPINELKGAVGVHFQDRTFSALGEESIVPI
                     THARSTGVFLLEERSWEKLRVEMGGRYEHATRNPQHNINPARSFDLFSGSIGGFWNLI
                     QDYNVGLTATHGQRAPAIEELYVNGAHHATATFQTGDATLRKEASNNIDLSINKATGP
                     IRWKINGFYNHFNDYIFLRNSDRDRNGIADRVDEEGILDPAGEFLVQDMTQRNATFYG
                     AEAEANLTLKPETLELRLFTDYVRGKLNSGGNIPRVTPQRFGMELNYVKGPWSGNLTT
                     IYVLRQNKRAELETSTADYVLLNLEASYRIKQTKADGIRLFIQGRNLLNEEIRYHTSF
                     LKNFVLQPGVALVVGIRGDF"
     gene            complement(1845..2720)
                     /locus_tag="PKZ49_07795"
     CDS             complement(1845..2720)
                     /locus_tag="PKZ49_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36501.1"
                     /translation="MFDDLAAYYHENVVSSFVEYRDVCNDGVAGRSRDLRAALNAASA
                     LFHLREHLPGGLLSRADVERLCPDYALLGDVVNASKHKTLNNKTPHGAPLVTDAGNLG
                     EQLVLIEYEDDAGTYRYIQKTVVVRLSDGSECSLLEILTSVINFWEGQMLSLEVLSKA
                     RVFTHDNDIRYRSRADCEANRLTFELVKGLRFHQTMRLLRFNKTTGRAEPIDLTGLDV
                     RSRIYQPKYDIELSLIHDASGKTYKTEITLTEEESLILSRMSSDEERRAYVNNLPSAQ
                     AALQQLAVGAGLPKG"
     gene            3093..3299
                     /locus_tag="PKZ49_07800"
     CDS             3093..3299
                     /locus_tag="PKZ49_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36502.1"
                     /translation="MQNFIGSFFRIHVDAFQHDLGADFDRVEVTGASHFGCTGTLFDN
                     IAFFIDQFIPQLLASRKRSVLDDV"
     gene            complement(3510..3887)
                     /locus_tag="PKZ49_07805"
     CDS             complement(3510..3887)
                     /locus_tag="PKZ49_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HND36503.1"
                     /translation="MSYTLLKLIHVTSVSISFCLFLLRGLWMVNASDMLKRRWVKILP
                     HVNDTVLLLSAIMLAITIQQNPLENSWLTAKVIGLLTYILLGMVALRFGKTRRTKITA
                     WIMALMVFGYIVLVALTKNPTIF"
     gene            complement(3887..4369)
                     /locus_tag="PKZ49_07810"
     CDS             complement(3887..4369)
                     /locus_tag="PKZ49_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36504.1"
                     /translation="MDEFALARVLHVLGVVLWIGGVAMVTTVLLPSVRQFKSEHEQIE
                     FFEQVESRFATQARFTTLLVGVSGFYMTYLLDTWSRFTEPRYWWMHAMVFVWVMFSIM
                     LYILEPLVLHKWFINRARVKPQATFNLILRMHWVMLSISLITTAGAVAGSHGWFPLGF
                     "
     gene            complement(4407..4760)
                     /locus_tag="PKZ49_07815"
     CDS             complement(4407..4760)
                     /locus_tag="PKZ49_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HND36505.1"
                     /translation="MINIEKTQNVVVVAVIGEFTLTDYKEFEDKVLHQYHFDGRANLL
                     FDWRDMVDYSVDVAWEEIKFMREHGSEFGRVAVVTDDQWQTWSAWVSNLFIDADVTVF
                     SDYDEALDWVTDAEV"
     gene            complement(4815..5666)
                     /locus_tag="PKZ49_07820"
     CDS             complement(4815..5666)
                     /locus_tag="PKZ49_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HND36506.1"
                     /translation="MHSLWMLVAGFMFACMGLFVKLGAAHFTSTELVFYRSLFGLIVT
                     FLFLTSCRMPLTTVHWKIHCWRGLFGLGGLLLFFYCLTQLPLATAVSLNNTWPLFLII
                     LATLILKEHLHWPLACAVMLGFTGVLLLLQPTLNENQLFAGLLGVISGFLASIAHFNV
                     KQLGKLGESDWLVVFYFTLISTIITGIWLTLTQFSTITPHNLLLLIGVGLTATLAQLA
                     LTRAYRMGKTMVIGALAYSTVLFSGLWDVYFWEEVLPISAWAGMGLIILGGILSIRVV
                     PKGIRNS"
     assembly_gap    5826..5925
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5926..6362)
                     /locus_tag="PKZ49_07825"
     CDS             complement(<5926..6362)
                     /locus_tag="PKZ49_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HND36507.1"
                     /translation="MSNPAELPHLTEKFLSLPIDWYLNQQIYEIEKQRLFEQGPGYIG
                     HELMIPNLGDYYVLKWLNNSKVLVRNEHGIELLSNICRHRQAILLEGHGNTKNIVCPI
                     HRWTYAMDGKLMGAPFFNRNPCLDLGKTILQHWNGMLFAGARNV"
     gene            6645..8030
                     /locus_tag="PKZ49_07830"
     CDS             6645..8030
                     /locus_tag="PKZ49_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HND36508.1"
                     /translation="MKLNQQTRKIGLIISGLCVLSLTACEDSKEVVEVKTKVTAKGPT
                     TGVLSDGFAISGVAYSTASGSSGVTDANGVFHYQHGDNVRFKLGELTLADIKGSAIVS
                     PIDLAAGNENKLRNLLILLQSLDSDDDVTNGISISSKVANAIKSSINLESDPATFSDN
                     AALKAAREAAELPGEAKSLEEAGEQFLARGINLLSSHIWVKYDNQSASALQIAEDGSG
                     EYLHGEATADDSCDENRVCGGETIFRTGVESGTTKAEAFDTRGFKLVGTTSIDTNLRA
                     GLSHPRATWRINTDGDELIISDIVVAQRERQQSSLFGELFHIAKPIELSADDEPIATE
                     VKERRYSKMDNTAQGIVGAWSLDKDTVKSKLFLFFPNNRFMLIDPTGQTVQSEPLACG
                     KPGIEFAAYSYDGASKALKITSFIFDTNACAGLSESADKLVTFDISSDGNTATLTKQG
                     QAAVKLSRVSN"
     gene            8145..>9478
                     /gene="priA"
                     /locus_tag="PKZ49_07835"
     CDS             8145..>9478
                     /gene="priA"
                     /locus_tag="PKZ49_07835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HND36509.1"
                     /translation="MTIVRVALNVPIDSLFDYRLPDASPQDIGRLVRVPFGKRRMVGV
                     IVERVQDTQIKPEQLKAVDEVLRSIPPLPALLLKLFIFCNHYYHHPIGMIAMGCLPAK
                     LAGVKSISKINTEQTFFMLTEAGRLVSINTLPVRDKAQRSLLTQLQTVGTLTSKQAKA
                     ISRRAPQLLGLFLTRAWIEEQHSVTPSIQPKRVSMPTLNAEQADAVKSIVAELHQFKT
                     WLLHGITGSGKTEVYLQVIAAVLQQDRQVLILVPEINLTPQLEKGFRDRFPALSVICL
                     HSKLNTTERTQNWLLAQAGTANIVLGTRLSIFTPLPKLGLIIVDEEHDPSYKQQDGFR
                     YSARDVAIFRAKQTGVPIVLGSATPSLESYHNALTGRYALLTLRTRAIKNAALPVIQL
                     IDTRNKLLQEGLSEPLLNALEQNLAQNKQSMVFINRRGYAPILLCKSCAWMAT"
BASE COUNT         2720 a         1946 c         2150 g         2562 t
ORIGIN      
        1 cctcggattc aagtattaga aaatggaatt ggaagtttgg atgtatcttc gctgagcccg
       61 gatcatcccg ttgccattga aaccctcaac gcatctcaaa tcgaaattct tcgtgggcca
      121 gcaacactac tatatggcgg gggggcgact ggtggggttg taaataccgt gaatagaaat
      181 attcccaacc aattgcataa aacactaacg ggtagttttg aagctcgagg aaatacagcc
      241 actgaggaaa gggccggttc ggtcaatttg agcagaagct ttggttcaat gagttggaat
      301 gtcagtggat ttagacgtaa caccggcgat tacaatattc ctggccaagc caatatcagt
      361 ggaattagtc cagaagctga aggcggctcg agtgcgcatt caattaataa tagaacccat
      421 gagttgaaaa atagtgctgc catgtcacaa ggcggatcgg ttggtggcag ttatgtcggt
      481 gaaagagggt ttctcggtgg ttcaatttca ctgatgaata gtaaatttgg tatcccgagc
      541 ttagaagcgc caaccatcga tatgacgcaa gcgcgttaca atcttgctgg agcgcttgat
      601 aatcccatta agggctttga aaaactcaaa gtacgaacgg gttacaatga ctacaagcac
      661 gacgaacgcg aacgtactgg agaattgggt acccgcttta aaaatcgtga actggaaaca
      721 cgcgttgaac tactgcacac gccgattaat gagctaaaag gcgctgtagg ggtgcacttt
      781 caagatcgga ctttttcagc gcttggggaa gaatccattg tgcccatcac ccatgcgcgc
      841 tcgacaggtg tttttttgct agaagagcgg agctgggaaa aactgagggt agagatggga
      901 gggcgctatg aacatgcgac tcggaatccg cagcataata tcaaccctgc tcgatcattt
      961 gatttattca gtggctccat tggtggattc tggaatttaa tccaagatta caacgtggga
     1021 ttgaccgcaa cacatgggca gcgtgcgcca gcaatcgagg agctttatgt gaatggcgcg
     1081 catcatgcaa cagcaacgtt tcaaactggt gacgctacgt tacgtaaaga agcatccaat
     1141 aacattgatt tatccatcaa taaagcaact ggcccaatac ggtggaaaat taatggcttt
     1201 tacaatcatt tcaacgacta catctttcta agaaattccg atcgcgatcg gaatggcatt
     1261 gccgaccgag tcgacgaaga gggaatcctt gatccggcag gggagttttt ggttcaagat
     1321 atgacgcaaa gaaatgcaac cttttacggg gcggaagcag aagcgaattt aacgcttaaa
     1381 cctgaaacgc tagaattgcg gctttttacc gactatgttc ggggtaaatt gaatagtggc
     1441 ggaaatattc cccgtgttac gccgcaacgc tttgggatgg aactcaatta tgtaaaaggc
     1501 ccctggtcag gtaatttgac gacaatctat gtgttacgcc agaacaagcg tgcagagcta
     1561 gaaacgagta ccgccgacta tgtattgctc aatcttgaag caagttatcg gattaagcag
     1621 accaaagcag atggcattcg gctttttatt caaggtagga atttgctcaa tgaagaaata
     1681 cgctaccata cctccttttt gaagaatttt gtactacaac ccggtgttgc ccttgttgtg
     1741 ggtataagag gtgattttta gaggagtgga gaacccgtgc gttcgatcga tttgttaaac
     1801 tatgcgggta ctttgttatg cgtcgcattt acggaggccc tcacttatcc tttcggaaga
     1861 ccggctccga ctgcaagctg ttgaagagcg gcttgtgcgg agggaaggtt gtttacgtat
     1921 gctcggcgtt cctcgtcgct tgacatgcga gaaaggatca aactttcttc ctccgtcagg
     1981 gtgatctcag tcttgtaggt cttcccgcta gcatcatgga ttagagacaa ctcgatgtcg
     2041 tacttgggtt ggtagatgcg agatcgcacg tccaagccag ttagatcgat aggctccgct
     2101 ctgcctgtcg ttttattgaa gcgcagaagt cgcatagttt ggtgaaatcg tagccctttc
     2161 accaattcaa aggtaaggcg gttggcttcg caatcggcgc gcgatcgata gcgaatgtcg
     2221 ttgtcgtgtg taaacacacg cgccttcgaa agcacttcca gagacaacat ttggccttcc
     2281 cagaaattga tgacactcgt aagaatttcg agcaggctac attctgaccc gtcagagagc
     2341 cttacaacta ctgtcttctg aatatatcgg taggttcctg catcatcttc gtattcgatc
     2401 aatacgagct gttcgcccag gttcccggca tcggtgacaa gcggtgcgcc atggggggtc
     2461 ttgttgttca gtgttttgtg cttcgatgcg tttacgacat cgccgaggag cgcatagtca
     2521 ggacaaaggc gctcgacgtc ggctcgcgac agtaacccac ctggcaaatg ctctcgtaga
     2581 tgaaagagtg ctgaggcagc gtttagtgcc gcgcggaggt cacgactgcg accagcaacc
     2641 ccatcgttac aaacatcgcg gtactcaaca aaagacgaga cgacattctc gtgataatag
     2701 gctgctagat catcaaacat ggcgattcag tctcgtgggg atgtcaagcg cccaacttca
     2761 attagacgtc cgaaatgacg caatatatta cgtcacaaat gatatctaat acggggttgt
     2821 tcatgatatt tccttatatt tctcagctaa tagccaattt agctgtcatt ttatcagact
     2881 tgtttagcgc cacaaattga atgaataatc aaacagaaaa aaacctcagt tttgagtcta
     2941 ctacgcaatg agagcaaata tccgagtaaa gaacgctgct caatcgtgca aattttcctg
     3001 tggtgacggg aaaaatatct aaataacagt tgctatgcat tattttggta aaaagtgaag
     3061 ttttttttcc ttcttaggga attttttccc ttatgcaaaa ttttattggc agcttttttc
     3121 ggattcatgt agatgctttt cagcatgacc tgggcgctga ctttgatcgt gtcgaagtaa
     3181 ctggcgcttc acattttggc tgcactggaa cactatttga taatatagcg ttttttattg
     3241 atcaatttat tccccagctt ttggcaagtc gaaaacggtc tgttcttgac gatgtatgag
     3301 ttcggctgct tgcttaagta ccgtgtgtgt ttctagctcg gttatgggag gcaaaagcgg
     3361 aagattgttg taaggttgat ccgggcgcca aactgtcatg gtttaatttc tgcatttttg
     3421 cgacacgttt taagtttatg tcgttcatgg cgacaggtca attcaatcta tcgcaaattt
     3481 ggcgtggctc agtaaatttc agggtattgt taaaaaattg tagggttctt ggtcaaagca
     3541 actaatacga tataaccaaa taccatcaat gccatgatcc aagcggtaat ttttgttctt
     3601 cgcgttttac caaaacgtag ggcaaccata ccgagtagga tgtaggtcaa taatcctatt
     3661 acttttgccg tcagccatga attttcaagt ggattttgct ggatagtgat tgctagcatg
     3721 atggcgctta gcagcaatac ggtatcgtta acatgcggta agattttgac ccaacgtcgc
     3781 ttcagcatgt ccgatgcatt gaccatccat agaccccgga gtaggaatag gcagaagctg
     3841 atcgatacac tcgttacatg gattagtttt aacaaggtat agctcatcag aaacctagag
     3901 gaaaccagcc atgactgcct gcaacggcac ctgctgtggt aattaagcta atactcagca
     3961 tgacccaatg catgcgaaga attaaattaa atgttgcttg cggtttcaca cgggctcggt
     4021 tgataaacca tttgtggagc acgagcggtt ctaggatata caacatgatt gaaaacatta
     4081 cccatacaaa aaccatcgca tgcatccacc agtatcttgg ttccgtgaaa cggctccaag
     4141 tatctaatag ataggtcata taaaaaccac tgacaccaac gaggagcgtt gtaaatcggg
     4201 cttgtgttgc aaaacggctt tcaacttgtt caaaaaattc tatttgctcg tgttccgatt
     4261 taaattgtcg tactgagggc agtaaaacag ttgttaccat ggctacaccg ccgatccaaa
     4321 gtacaacgcc aagcacatgt aagactctcg ctagcgcaaa ttcatccata agccttttgt
     4381 tcagctcaac tgattaggtg ggttatttaa acttctgcgt cagtaaccca gtccagcgct
     4441 tcatcgtaat ctgaaaaaac ggttacatca gcatcgatga aaagattgga aacccaagcg
     4501 ctccaggttt gccattgatc atctgtcaca acggcaacac gaccgaattc gctgccatgt
     4561 tcacgcataa atttaatttc ttcccaagcg acatcgacag agtagtcaac catgtcacgc
     4621 caatcgaata acaaattagc acgaccatcg aaatggtatt gatgtaaaac tttgtcttca
     4681 aactctttat aatctgtgag cgtaaattcc ccaataacag cgacaaccac aacgttttgg
     4741 gttttttcaa tattgatcat gatttaaata ctcccaagtt ctatttttat gaatgggtac
     4801 cacgcatccg ttcattagga attgcggata cccttgggca ccactcggat gcttaatatt
     4861 ccacctaaaa tgattaatcc cattcctgcc catgctgaaa taggcagtac ttcctcccaa
     4921 aaataaacat cccatagtcc agagaataat accgtactat aggcgagtgc accaatgacc
     4981 attgttttac ccattcgata agcgcgtgtc aaagccaatt gcgctaacgt tgcggttagg
     5041 ccgaccccaa tcaataacag cagattatgc ggtgtaatcg tactaaattg ggtcagagtc
     5101 aaccaaatgc cggtgataat ggtcgatatc aaggtgaaat aaaaaacaac gagccaatcg
     5161 gattcaccga gttttcctaa ttgttttaca ttgaaatgcg cgatactggc caaaaaaccg
     5221 gaaataacgc ctaacaaacc agcgaatagt tgattttcat taagcgtagg ttgtaatagt
     5281 aataaaacgc cagtaaagcc cagcattacc gcgcaagcga gcggccagtg taaatgttct
     5341 ttgagtataa gggtcgctaa gataattaaa aataagggcc aggtattgtt cagagaaact
     5401 gccgtcgcta agggaagctg agtgaggcaa taaaaaaaga gtagcaatcc gcctaatcca
     5461 aacaatccac gccaacaatg aattttccag tgcacagtcg ttaatggcat acggcaactg
     5521 gtcagaaata ggaacgtaac gattaagcca aacagtgaac ggtaaaatac taattcagtg
     5581 ctggtaaaat gtgccgcgcc taatttcaca aacaagccca tgcaagcaaa catgaagcct
     5641 gctacgagca tccataatga gtgcatggga aattaatcgc ttacaaaagg aaaatgatga
     5701 aggaaactgt aagtctttta gcggataaag ggaccaattt cgcgtcgtag gaactgatga
     5761 aaatgggcca tgccttcttc cattgggatt tgataaggac caaattcatt gatttgttgc
     5821 tgatannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnacgtt tcttgcgcca
     5941 gcaaataaca ttccattcca gtgctgtaaa atggttttac ccagatctaa gcaggggttt
     6001 cgattaaaaa aaggggcacc cattaacttt ccatccatcg cataggtcca tcgatggatt
     6061 gggcaaacga tatttttggt attaccatgg ccttcaagca aaatggcctg acgatggcgg
     6121 caaatatttg agagcaactc aataccatgt tcattacgca ctaatacttt tgaattgttc
     6181 agccatttca agacatagta atcacccaga ttaggaatca tcaattcgtg gccaatataa
     6241 ccgggaccct gttcaaacaa tcgttgtttt tcaatctcgt aaatctgctg attgagatac
     6301 caatcgatag gaagtgacag aaatttttcg gttaaatggg gcaattcagc aggattcgac
     6361 ataaacaatc agttaattat caaatgatgt tatcaacttt caaagtttga ttgaccaact
     6421 aagtgtataa gcattcaaaa aatcaaaaaa ataaattaca tctatacttt ttgctggatg
     6481 tgaatttatc attcagctag gttttgtttt gagggttata ataatcctaa agattaacaa
     6541 aaaatctgag cgcgatatta tcctattatt tcataagatt acaattttag gtacgctata
     6601 catatcgcat ctattgatta actaaattgg gaagagtcac tttgatgaaa ttgaatcaac
     6661 aaacaagaaa aatagggtta attatatctg gtctatgcgt tttaagcctg actgcttgtg
     6721 aggatagtaa agaagttgtt gaggtgaaaa caaaagtgac cgcaaagggg cctacgactg
     6781 gagttcttag cgatggattt gcaatttcag gtgtggctta tagtacggct tctggttcat
     6841 caggcgttac cgatgcaaac ggtgtttttc actatcagca tggcgataat gttcgcttca
     6901 aactgggtga attgaccctc gcggatatca aagggtcagc aatcgtaagc ccgattgatc
     6961 tggctgccgg taatgaaaat aaactaagga acttgctgat tttattgcaa tcactggatt
     7021 cagatgatga tgtcaccaat ggtatttcaa tttcatccaa agttgccaat gcaatcaaat
     7081 cgtccattaa tttggaatct gatccagcta ccttttctga caatgctgct ctcaaagctg
     7141 cgcgtgaagc cgctgagtta ccaggggaag ctaaatcatt ggaggaagcg ggcgaacaat
     7201 ttctggcccg gggtattaat ctgttaagca gtcatatttg ggttaaatac gataaccaaa
     7261 gcgcaagcgc ccttcaaatc gctgaagatg ggagtggtga gtacttacat ggagaagcga
     7321 ctgcagacga ttcctgtgat gagaatcgtg tttgtggtgg tgaaacgatt tttagaacag
     7381 gtgtggagtc aggaacgacc aaagctgaag cttttgatac acgcggattt aaactggtgg
     7441 gaacaacatc aattgatacc aaccttagag caggattatc acatccccgt gcgacttggc
     7501 gaattaatac cgatggtgat gaattgatta tctcggatat cgttgttgca caacgcgaaa
     7561 gacaacaaag tagtttattt ggtgaattat ttcatattgc taaaccgatt gaacttagcg
     7621 cggatgatga gccgatcgca acagaggtaa aagaacgccg ttattccaag atggataata
     7681 ccgcacaggg cattgttggc gcatggagtt tggataaaga tacggtcaaa tcaaaattgt
     7741 tcttattttt tcccaataat cgatttatgc tgatcgaccc gacaggacag acggttcaat
     7801 cagaaccgct cgcgtgtggt aaaccgggta ttgaatttgc tgcgtatagt tatgatggcg
     7861 cgagtaaggc attaaaaatt acaagtttta tttttgatac caacgcctgt gccggcttat
     7921 cagaaagtgc tgataaattg gttacttttg atatcagctc agatgggaat acggctactt
     7981 tgacaaagca gggtcaagca gcggtgaaat tgtctcgtgt atcgaattaa tgttgtgagc
     8041 atttaattct cgatcttact gattcattaa aaaggcaaga gtaaatctct tgcctttttg
     8101 ttttctaaaa ctaatcttgt agtgatgtga ttgagtaacg ataagtgacg attgttcgag
     8161 ttgcactcaa tgtcccgatt gatagtttgt tcgattatcg gttacccgat gcttcgccac
     8221 aggacatcgg ccgattggtt cgcgttccat ttggaaagcg gcgtatggtt ggtgttattg
     8281 tggagagggt tcaagatact cagattaagc ctgaacaatt aaaagcggtt gatgaagttt
     8341 taagatcaat tccgcctctg ccagccttgt tgcttaagct gtttattttt tgtaatcact
     8401 attaccacca tccgatcgga atgattgcca tggggtgttt gcctgcaaag ctagcaggtg
     8461 tgaaatcgat aagtaaaatc aatactgagc aaacattttt tatgctcacc gaggcaggcc
     8521 gattggtatc gatcaataca ctgccggtac gtgataaagc gcaacgtagt ctacttacac
     8581 agctgcaaac agtgggcacg ctgacaagca agcaagctaa agccatttca aggcgtgcac
     8641 cgcagctact agggctgttt ttgacacggg cgtggattga ggaacaacac tctgtgaccc
     8701 cgtcgattca gcctaagcga gtatcaatgc cgacgctcaa tgctgagcag gcagatgcag
     8761 tcaaatccat tgtggcagag cttcatcaat ttaaaacttg gttattgcat gggattaccg
     8821 gcagtggtaa aaccgaagtc tatctgcagg ttatcgccgc tgtcttacaa caagatcgcc
     8881 aagtattaat tttagtgcct gaaatcaatc taacccctca gcttgaaaaa gggtttcgag
     8941 atcgctttcc tgctttatcg gtgatttgtc tgcacagtaa actgaatacc accgagcgca
     9001 ctcaaaactg gctgctagct caagccggta cggctaacat cgttcttggg acacggcttt
     9061 cgatatttac gccattaccg aaactaggcc tgatcattgt cgatgaagag catgacccat
     9121 cctataaaca gcaagatggt tttcgctatt cagcgcgcga tgtggcgatt tttcgtgcca
     9181 aacagacggg tgttcctatt gttctaggtt cggctacgcc ttccctggaa agctatcata
     9241 atgccttaac agggcggtac gcgttattaa cgctaagaac gcgggccatc aagaatgcag
     9301 cattacccgt gattcaattg attgacacgc gtaacaaatt gcttcaagaa ggtttgtctg
     9361 aaccgttgtt gaacgcgctt gagcaaaatc tggcgcaaaa caagcaaagt atggtgttta
     9421 ttaatcggcg aggttatgca cccattctcc tgtgcaaatc atgcgcctgg atggcgac
//
LOCUS       DAOQKQ010000102         9396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_3474_length_9396_cov_5.986511, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000102 DAOQKQ010000000
VERSION     DAOQKQ010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 9396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9396
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3474_length_9396_cov_5.986511"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="PKZ49_07840"
     CDS             complement(<1..836)
                     /locus_tag="PKZ49_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HND36510.1"
                     /translation="MLDIQQLRNELDLVAVKLAKRGYEFPVDQFRSLELKRKVIQTET
                     QELQAKRNAVSKQIGIAKQKGEAVTAMMAEIANLGDSLKQFENQLEHIQNELQKILLV
                     TPNLAHESVPEGRDDKNNLEIRRWGKPKLFDFEIKDHVTIGEQLGMLDFETASKLSGA
                     RFNVMKGGIARLHRALAQFMLDTHTLEHGYTEVYVPYLVNADCLRGTGQLPKFAQDLF
                     VAQSGAEIKSDSDLADQGIGKLHLIPTAEVPLTNMVSDKIIPCEQLPLKFVAHTPCFR
                     SEA"
     gene            complement(946..1473)
                     /locus_tag="PKZ49_07845"
     CDS             complement(946..1473)
                     /locus_tag="PKZ49_07845"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HND36511.1"
                     /translation="MPIKSLIRTIAHYPHEGIQYRDITTLLKDPIGLKTTIEEIAKRY
                     QLMKIDKVVGIESRGFIIGAPVAYQLEIGFVPIRKSGKLPAKTIGCEYDLEYGTDRIE
                     LHTDAIQPGERVLLIDDLIATGGTMEAAVKLIHELGGEVVECCFVVDLPEAGGRLRLE
                     KQGQKVFSLCQFEGS"
     gene            1762..2973
                     /gene="zapE"
                     /locus_tag="PKZ49_07850"
     CDS             1762..2973
                     /gene="zapE"
                     /locus_tag="PKZ49_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HND36512.1"
                     /translation="MKQTTKQSEGPEAEYQALLQAGELQPDIAQRRAIHALERLHHEL
                     FAYSEVTNRGSQRSFLSWWPFRALTRNTNNLTAPKGVYLYGGVGRGKSMLMDVFYSVT
                     PTTQKKRVHFHEFMLEVHARLKAINEHSAQKRKRESRYLSGDNDPIPLIAQEIADETV
                     LLCFDELHVTDIADAMVLTRLFDKLFELGVVVVATSNRLPDDLYKNGLNRQRFLPFIE
                     QIKEKLHIIALDGPVDYRYNRLKGAQTYYFPINEDATNQLAITFFQLTDRRIEDRDKV
                     PSEELIVQGRKLFVPKAAKGVAVFSFKRLCANPLGTADYLAIARTYHTVIMVAIPQFS
                     QETEDQARRFVLFIDALYEYSVKFLCSAAVPVQLLYTGSEISFEFERTISRLMEMQSE
                     DYLTQGHGKAI"
     gene            2989..3321
                     /locus_tag="PKZ49_07855"
     CDS             2989..3321
                     /locus_tag="PKZ49_07855"
                     /inference="COORDINATES: protein motif:HMM:NF018784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HND36513.1"
                     /translation="MRKIRTLVLVYFAILILSGCGTLTTVFQADSVTKRKLNQVETKC
                     ELVPRIYSGIAYDFCVLRGKPSQAALWLVPVPELIMVDIALSAALDTALLPYTIYEQI
                     QSGSIYIQ"
     gene            3409..3615
                     /locus_tag="PKZ49_07860"
     CDS             3409..3615
                     /locus_tag="PKZ49_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36514.1"
                     /translation="MSKLSILVTWSEARWVKKGDKSVFGYEKLTGLASNGLLMAVLAT
                     AVNCHDSELLARLERLPNSLPNTE"
     gene            complement(3857..4210)
                     /locus_tag="PKZ49_07865"
     CDS             complement(3857..4210)
                     /locus_tag="PKZ49_07865"
                     /inference="COORDINATES: protein motif:HMM:NF025062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CZB domain-containing protein"
                     /protein_id="HND36515.1"
                     /translation="MDLNQAIEKHAEWKVKFRSAIVRKESMDAGTISKDNCCELGKWL
                     HGIGRAKFSNLPSFKNCVLNHVRFHIEAGKVAQAINLKKYTEAETMLSNGTPYSLASN
                     EVGTAIIQLKKEAQI"
     gene            4608..5369
                     /locus_tag="PKZ49_07870"
     CDS             4608..5369
                     /locus_tag="PKZ49_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HND36516.1"
                     /translation="MMHFFKLLTIYKRIKEIVFQIGYINTAILLFHRILIKLFSGKLR
                     IYKYYLTAQPVTNRTFLPPNRGKKITVKQISRDDPIVSAFPRPDSIIKNRFDTGAVCL
                     VALKENVFVGYIWFMLGAYQEDEVRAKFVPLPASATVWDFDIYVHPDYRLGHTFLRLW
                     DEANRFLLDRKIEWSCSRISAFNAPSINVHTKLGALNLGQVIFFCIGSCQLTLATLFP
                     YIHVSTHLNSYPTFFIDTQKNNTIFPMKGKESTTE"
     gene            5374..5631
                     /locus_tag="PKZ49_07875"
     CDS             5374..5631
                     /locus_tag="PKZ49_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HND36517.1"
                     /translation="MQHIDEVRNILADTLSLGDRKHQLNENSFLLGSIPELDSMAVVN
                     VITALEDFYNISVDDDEISAKTFETLGSLTIFVEQKLSQSN"
     gene            5633..6469
                     /locus_tag="PKZ49_07880"
     CDS             5633..6469
                     /locus_tag="PKZ49_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase 2, exosortase A system-associated"
                     /protein_id="HND36518.1"
                     /translation="MDSPTLCAEPFFLKVESGERFCLFYRPNLANPLGTFIFIHPFAE
                     EMNKSRRMIAQQSKAFSQNGFAVLQIDLYGCGDSSGEFNEARWEVWKQDIFHAINWIK
                     HQTAAPINLWGLRLGGLLALDLASGAEEFFQHIVLWNPIISGNTFLTQFLRLKVASQL
                     MIKSGKESVGTGALRNKIMAGETLEIAGYELSSILAIAIDKLRIGDFKVFDTKIHWFE
                     VVPEAGRVLSPSAMATVNSWKERNIDLDLQYVTGQAFWATQEIAECPELIEATINQFS
                     TV"
     gene            6472..7341
                     /locus_tag="PKZ49_07885"
     CDS             6472..7341
                     /locus_tag="PKZ49_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase 1, exosortase A system-associated"
                     /protein_id="HND36519.1"
                     /translation="MNFSEQPLRFCCQGCWLYGILSIPFQSCKRGVLIVVGGPQYRVG
                     SHRQFTLLARFLADHDIPAFRFDYRGMGDSDGVIRTFENIGDDLESAIDQFFINVPSL
                     KEVVIWGLCDAASAALFYAHKDQRVTGLVLVNPWVRTESGVAKAYLKHYYTSRLLDGE
                     FWGKIIRGDFKLFDSFKFLFQSIKKSLIRKTEIANDKIKQSTCNLLLPLPQRMLEGLK
                     QYTGKIFIITSGDDLTAKEFLDLINTTKDWRQIINIKQVKFAHLKDANHTFSSRQWRD
                     QVAVWTTNWTKSW"
     gene            7335..8573
                     /locus_tag="PKZ49_07890"
     CDS             7335..8573
                     /locus_tag="PKZ49_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HND36520.1"
                     /translation="MVRRVLLIAYHFPPLQGSSGIQRTLRFAQYLPKLGWEPIILTAH
                     PISYQLTRPTQLPKEFDNISIYRSFALDTARHLSLLKRYPRMLAVPDRWISWWLGAFP
                     QGLYLIKKFKPDVIWSTFPIATAHLIGYTLSRISGIPWAADFRDPMVQRDYPTDPIIY
                     RAYQWIEEKTIRNCASVIFTTANTRKDYISRFSTESESRFKVIENGYDEQLFSEVTVE
                     HQVKQNKQLTLVHSGIIYSSERDPTYFFEALSDLLKEKYISSDNLKIILRATYNDNFL
                     QQLINRFNIGSIVTLAPPISYKEAIAEMLSADGLIILQASNCNNQIPAKLYEYLRAQK
                     PILALTDPEGDTGLKLISLGFDTISRLDSKPDIKELILKFLDSIRNKIVQPLPLNQLQ
                     LHSRESRTSELVEILNGIVR"
     gene            8636..9268
                     /locus_tag="PKZ49_07895"
     CDS             8636..9268
                     /locus_tag="PKZ49_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HND36521.1"
                     /translation="MKLLCHISFFLSIIALSFQAYSQQNSICGELKGPHYGPFDYLER
                     VQLRDKLEIVEAYHFSQNVEKLKGNVTSVAADLSYTLHAWPNHHRALASLAKLAIREK
                     AVNLPGLKYNVECYFDRAFRWRSNDSIVRSIYGSYLSKVGRANNAIEQLEIAVRLKSD
                     NATAHYNLGLLYFDKANYDKAQFHAESAYALDFPLPGLRNKLIRAGKWKK"
BASE COUNT         2817 a         1690 c         1891 g         2998 t
ORIGIN      
        1 gcttctgagc ggaagcaggg agtgtgtgcg acaaatttta gcggaagttg ctcacaagga
       61 atgattttat cactgaccat attcgttaaa ggtacttctg ctgtggggat taggtgcaat
      121 ttacctatac cttgatcagc gagatctgag tcagatttaa tttctgcacc tgactgggca
      181 acaaataagt cttgtgcaaa tttgggcaac tgccctgtgc cgcgcaaaca atcggcattc
      241 actaaataag gcacatagac ttcagtgtaa ccatgctcaa gcgtgtgggt atctaacata
      301 aactgggcta gcgcccgatg taagcgagca ataccgcctt tcatgacgtt aaaacgagcg
      361 ccacttaatt tagaggctgt ttcgaaatca agcataccta attgttcacc aatcgtgaca
      421 tgatctttaa tctcgaaatc aaatagttta ggttttcccc agcgtcttat ctcaagatta
      481 tttttatcat ctctgccttc aggtacgctt tcatgtgcca aattaggggt taccaataat
      541 attttttgca gttcattttg gatatgttca agttgattct cgaattgttt aaggctatca
      601 cctaaattgg cgatctctgc catcattgct gtgactgctt cccctttttg cttggcaatc
      661 ccgatctgct ttgagactgc attccttttt gcttgcagct cctgtgtctc ggtttgaatt
      721 actttacgtt ttagttcaag tgacctgaat tggtcgactg gaaattcata gccacgtttg
      781 gctaatttta ctgccacaag gtcaagttcg ttacgaagtt gctggatatc taacatcctt
      841 gatgggtacc ttattaatga tgagtgataa agcgttagtt ttttttactt aatgaatcgt
      901 tatcaaatga aaaattacaa ctaacattaa taaatcattt ctcagttagc tgccttcaaa
      961 ttggcaaagt gaaaacactt tttgtccctg tttttctaag cgtaaccttc ctcctgcttc
     1021 gggtagatca actacaaaac agcattcgac aacttctccg cccaattcat gaattaactt
     1081 aacagctgcc tccatggttc cacccgttgc aattaaatca tctatcaaca acacgcgttc
     1141 ccccggctgt attgcatcgg tatgaagttc gatacgatct gttccatatt ctaaatcata
     1201 ttcgcatcca atagtcttgg ctggcaattt gccagatttc cgaatgggta caaaaccaat
     1261 ttctagttga taagcgactg gagcaccaat gataaatccc ctggattcga ttccaaccac
     1321 tttgtcgatt ttcatcaact gataccgttt tgcaatttcc tctatggtag tttttaaccc
     1381 aataggatcc tttagcaagg ttgtaatatc tcgatattgg atcccctcat gtggataatg
     1441 ggcaatcgtc cgaattaggg attttatggg catataaagt taataaaata atatttgctt
     1501 gcgaaataac taatcatttt atcgtttttt aaacttgacg gagaagatga ttctgcgatt
     1561 tataaaaaat ctgttgatga caaaaaaatt cagtctgtta ttagatagta gcatcaggaa
     1621 gcattacgat catttatcag tgaatcaatc catgtgatgt ttaaagcaga gctaaccggg
     1681 aattgatcta ctgttagctt tttatcagta tgattcagat cattatattt gaacaattca
     1741 gatagcctat atcgaagtac catgaaacaa actactaaac agtccgaagg gcccgaagcg
     1801 gaatatcagg cattattgca ggcaggtgaa ttacagcctg atatagcaca gcgtcgagcg
     1861 attcatgcat tagagcgatt gcaccatgaa ttatttgctt attctgaagt aacgaataga
     1921 gggagccaac gatctttttt gtcatggtgg ccttttagag cactaacaag aaatactaac
     1981 aatttgactg ctcccaaagg agtttatctt tatggtggcg ttggtcgtgg aaaatcaatg
     2041 ctgatggacg tgttttactc agttactccg accactcaga agaaacgcgt ccattttcat
     2101 gaatttatgc tggaagttca tgcaagactg aaagcaatca atgagcattc tgctcagaag
     2161 cgcaaacggg aatcacggta cttgtcaggt gataatgatc cgattccact gattgcgcaa
     2221 gaaatcgctg atgagactgt tttgttgtgt tttgatgaat tacacgtgac cgatattgcc
     2281 gatgcaatgg tgttgacgcg cttattcgat aagttattcg aattgggagt tgttgtggta
     2341 gcgacttcta accgacttcc tgatgatctc tataaaaatg ggttgaatcg acaacgtttt
     2401 cttccattta ttgagcaaat caaagaaaag ctgcatatta ttgcattaga tggcccggtt
     2461 gattatcgct ataatcgttt gaaaggcgct cagacttact attttccgat taacgaggat
     2521 gcaactaacc aattagccat tacttttttt cagttaactg atcggcgtat tgaagatcga
     2581 gataaagtac cgagtgaaga actcattgtg caaggaagaa aactgtttgt tcctaaagcg
     2641 gctaaggggg ttgcggtttt ctcgttcaag cgattatgtg ctaacccttt aggtaccgct
     2701 gattatttag cgattgctag aacgtatcat acggtaataa tggtcgcaat accgcaattt
     2761 agtcaggaaa ctgaagacca agccaggcgg tttgttttat ttatcgatgc gttgtatgag
     2821 tatagtgtta aatttctctg ctcggcagca gtgccggttc aattgcttta taccggtagt
     2881 gaaattagtt ttgaatttga gcgtacgatt tcacggctga tggaaatgca gtcagaagat
     2941 tatttaacac agggccatgg caaagccatt taaaattaat attaaagtat gagaaaaatt
     3001 cgaacgctgg ttctagtgta ctttgctatt ctgattctct cgggatgcgg caccctgact
     3061 actgttttcc aagcagatag cgttacaaaa cgtaaactga atcaagttga aactaaatgt
     3121 gaattggtgc ccagaattta tagtggaata gcgtacgatt tttgtgtatt gcgtgggaag
     3181 ccatcgcaag ctgcgctgtg gttagttccg gtacctgagt taataatggt cgatatcgcc
     3241 ctatcagcag cattagacac agctctatta ccttatacga tttatgagca aatccaaagt
     3301 ggtagtattt atattcagtg aaagtcctat tacctgaatt ctgcgatcat ttctttgagt
     3361 agttaacaat gtcaggaaaa tttcttgtca accttgtctc gatgaaaatt gagtaaactt
     3421 agtattttag tgacctggtc agaagcacgc tgggttaaaa aaggcgacaa atctgtattc
     3481 ggttatgaga agcttacggg attagctagc aatgggttgt tgatggcagt attagctact
     3541 gccgtcaatt gtcatgatag cgagttgctt gctcgacttg aaagacttcc caactctttg
     3601 ccgaacactg aatgactccg caaatatagg agatagtgcg cctactctac cggaaatagc
     3661 cgatttagat ggactaatta caagcttgtt cgtggaaaaa ttactaaaaa gcccaattcc
     3721 ccaacaaatt agcccggaat ttttctagaa tgcaaagttt gcgttatttt gcaaagattt
     3781 cggttagtct atagaaataa ccaaaactaa tcataattca atatagccca ctctgatgtg
     3841 ttttagagca tttttcctat atttgtgctt ctttcttgag ttgaattatt gcagttccaa
     3901 cctcatttga tgctaggctg tatggggttc cattgcttaa catcgtttct gcttcagtgt
     3961 atttttttaa attgatagct tgagctacct ttcctgcttc aatatgaaat ctcacatgat
     4021 tcaaaacaca gttcttgaaa cttggtaaat tactaaactt agctctaccg ataccatgca
     4081 accatttccc caattcacag caattatctt ttgaaattgt tccagcatcc atagactctt
     4141 ttctgacaat tgcacttcta aatttaactt tccattctgc atgtttttca atcgcttgat
     4201 ttaggtccat aatattctca ctggtaaata atcaagctaa ttacgacagt aattgctgta
     4261 aaatttagag atggcctcgt ttattaaaag attgcatgta aagcacttta aaattggtag
     4321 agaaaacggt agggatttaa aatgaataat atgaggatcg gcttaatgaa tgtttttcat
     4381 gatagatatg aatcccttcc gccgttgtgg gtaaataaaa tttaactagg ctaacgttaa
     4441 aaaagggatt tatcacatgc ctattgtttt gtttaattaa gtaaaactga tatacgataa
     4501 gtattcaaga ttaataaata aaaaatgaga tttagcaata tttttaccct agatcgtagg
     4561 gcggctttta attttttaag tatattatga caagatgagc tgcgtggatg atgcatttct
     4621 ttaagctatt aacaatttat aaaaggatta aagagatcgt tttccagata ggctacatta
     4681 atacagcaat tctcttgttt catcgaattc tcataaagct atttagtggt aagctacgta
     4741 tatacaaata ttatttgact gcccaaccag ttactaatcg aacttttcta ccacccaata
     4801 ggggtaaaaa aattacggtg aagcaaataa gtcgtgatga tcctattgtt agcgcttttc
     4861 caagacctga ttcaataatc aaaaatcgct ttgatactgg cgctgtatgc ctagttgcct
     4921 tgaaagaaaa tgtttttgtt ggctacattt ggtttatgtt aggagcctat caagaagatg
     4981 aagtacgggc aaaatttgtt ccattacctg cctcggctac tgtttgggat ttcgatattt
     5041 atgttcatcc agactatcgt ttagggcata cctttttacg tttatgggat gaagctaatc
     5101 gattcttgct cgatagaaaa atagaatggt cgtgtagtag aatatcagcc tttaatgcgc
     5161 cttcgataaa tgttcataca aaactagggg cattaaattt agggcaagtt atcttttttt
     5221 gtataggtag ttgtcaatta acgttagcaa cacttttccc gtatattcac gtttcaacac
     5281 accttaactc atacccaact tttttcatag atacacaaaa aaacaatact atttttccta
     5341 tgaaagggaa agaatcaaca acggagtaaa tttatgcagc atattgatga agttagaaat
     5401 atcttagcag atacgcttag tctcggtgat aggaagcacc agctcaatga gaattctttt
     5461 ttgcttggca gcattcctga attagattca atggcagtag taaatgtaat taccgcactt
     5521 gaagattttt acaatatatc ggttgatgat gatgaaatta gtgcaaaaac ctttgagacc
     5581 cttggtagct taactatttt tgtggaacaa aaattgtcac agtcgaacta gaatggattc
     5641 tccaactctt tgtgcggagc cgttcttttt gaaagttgaa tcaggggaaa ggttttgcct
     5701 attttatcga ccaaatcttg ctaatccatt gggtaccttt atttttattc atcctttcgc
     5761 agaagagatg aataaatcac gaaggatgat tgcacaacag tctaaggcgt tttctcaaaa
     5821 tggttttgca gtgcttcaaa ttgacttgta tggatgtggc gatagtagtg gtgaattcaa
     5881 tgaggctcgt tgggaagtct ggaaacaaga tatttttcat gcaatcaatt ggattaaaca
     5941 tcaaacagct gccccgatta atctttgggg actacgtttg ggtgggttac ttgcgcttga
     6001 tttagcgagt ggggctgaag aatttttcca acatattgtt ttgtggaatc ctattatcag
     6061 tggaaatact tttctaacac aattcttgag gttgaaagta gctagccaac ttatgataaa
     6121 aagtggcaaa gaaagtgttg ggacaggtgc tctacgtaat aaaataatgg cgggtgaaac
     6181 acttgagata gctgggtatg agctttcttc gattcttgca atagctatcg acaaattaag
     6241 gataggcgat ttcaaagtat ttgatactaa aatccactgg tttgaggtag tgcccgaagc
     6301 tggcagagta ctatcacctt cagcaatggc gacagtcaat tcctggaaag agcgcaatat
     6361 cgatctggat ttgcaatatg taacaggaca ggcattttgg gcaactcaag aaatcgctga
     6421 gtgtcctgag ttaattgaag ccacgataaa tcaattttcg actgtttaat aatgaatttt
     6481 agtgagcaac ccttgcgttt ttgttgtcaa ggttgttggt tgtatggaat tcttagcatt
     6541 ccgttccaat catgtaaaag gggggtactg attgttgtgg gaggacctca atatcgggtt
     6601 ggaagtcatc gccaatttac actccttgcc cgttttctcg ctgatcatga tattcctgct
     6661 tttcgctttg attatcgagg aatgggggat agcgatggtg taattcgaac gtttgagaat
     6721 ataggcgatg atttggaaag tgcgattgat caattcttta ttaacgtgcc ttcacttaaa
     6781 gaagtggtaa tttgggggtt gtgtgacgca gcatcagcgg ctttattcta tgctcataaa
     6841 gatcaaaggg ttactgggct tgtattagtt aatccttggg ttagaactga atctggcgtc
     6901 gcaaaagcct atcttaaaca ctattatact tcgaggttgc tagatgggga gttctgggga
     6961 aaaattataa ggggagattt taagcttttc gattcgttta aatttttatt tcaatctata
     7021 aaaaaatcct taataaggaa aacagaaata gcgaatgata aaattaagca atctacttgt
     7081 aacttgttac taccattacc gcagcggatg ttggagggtc taaagcaata taccggaaaa
     7141 atattcatca ttaccagcgg agacgatcta acagcaaaag aatttctaga cttgatcaat
     7201 actacaaaag attggcgcca aataatcaat attaagcaag ttaaatttgc tcatttgaag
     7261 gatgcaaacc ataccttttc ttctcgtcag tggcgcgacc aagtagcggt ctggacaact
     7321 aattggacaa aatcatggta aggcgtgttc tgctgattgc gtatcatttt cctccattac
     7381 aaggaagcag tggtatacag agaacattaa gatttgcaca gtatcttcca aaattaggtt
     7441 gggaaccaat cattttaact gctcacccaa tttcctatca gcttactagg cctacccagt
     7501 tacctaagga atttgacaat atcagtatct atcggtcttt tgcgctggat acggcaaggc
     7561 atttatcact gctaaagcgt tatccaagaa tgcttgctgt gcctgatcga tggatttctt
     7621 ggtggcttgg ggcgtttccc caagggcttt atttgataaa aaaatttaaa ccagatgtca
     7681 tttggtcaac attccccata gcaacagcgc atcttattgg ttatacactg agtcggatat
     7741 ctggtattcc gtgggcggct gattttcgtg atccaatggt acaacgcgat tatcctactg
     7801 atcccatcat atatcgcgct tatcaatgga ttgaagaaaa aacaattcgt aattgcgcca
     7861 gcgtaatttt tactacagct aacacgagga aagattacat cagtcgattt tctactgaaa
     7921 gcgaatcgcg ctttaaagtt atcgaaaatg gttatgatga gcaacttttc tcagaagtta
     7981 cggttgaaca tcaagtaaaa caaaataaac aactaacgct tgttcatagc ggcattatct
     8041 attcgtctga gcgtgatcca acctactttt ttgaggcgct atcagattta ctaaaagaaa
     8101 agtatatttc ttctgacaat ctaaagatta ttcttcgagc aacttacaac gataattttt
     8161 tacaacaact gattaatcga ttcaatattg gatcgatagt tacgctagct ccaccgattt
     8221 cgtacaaaga agcaattgca gaaatgttat ccgcagatgg gttaattatt ttgcaggctt
     8281 ccaactgtaa taatcaaata cctgcaaagc tatatgagta tttacgtgcg cagaaaccga
     8341 ttctggcact aactgatcca gaaggggata ctggtctaaa acttataagc ttaggttttg
     8401 atacaataag tcggttagac tctaaaccag atatcaagga attaatatta aagtttcttg
     8461 actctattag aaataagatt gttcaacctt taccgttaaa ccaacttcaa ctgcactcaa
     8521 gagaatcaag aacatctgag ttagtggaaa ttttgaatgg aatcgtaagg tgaccaatga
     8581 tttatttttt agcattaatt aatgatatat agaagttact caagaggcga tttatatgaa
     8641 attattatgc catatcagtt tcttcttatc gattatagcc ctatcatttc aagcttattc
     8701 tcagcaaaat agtatttgtg gggagttaaa gggtccacac tatggacctt ttgattatct
     8761 tgaaagagtt cagttaagag acaagcttga aatagtagag gcctatcatt tttcacagaa
     8821 tgttgagaaa ctaaaaggta atgtaacgag tgttgcagca gatttaagct ataccttgca
     8881 tgcctggccg aaccaccatc gagccttggc atctttagca aaactcgcga ttagagagaa
     8941 ggctgttaac ttacctgggt tgaagtacaa tgtagaatgt tattttgatc gtgcttttcg
     9001 ttggcgatca aatgatagta ttgttagatc gatttatgga agttatttat caaaagtcgg
     9061 tagagctaat aatgcaattg aacagctcga aattgccgtg agattaaagt ctgataacgc
     9121 tacagcgcac tacaatcttg gattgcttta ttttgataaa gctaattatg ataaagcaca
     9181 atttcatgct gagagcgctt atgcgcttga ctttcctttg cctggtttac gaaataaact
     9241 tataagagct gggaaatgga agaagtagga agatcatggc ggacgtgcgt cacttttcct
     9301 aataaaaagt aaaaccggta gaaaataatt acgcctcatc ggcatctggg ttttcagtat
     9361 ggtcgagatt tatatcctct actgggacag aagttt
//
LOCUS       DAOQKQ010000103         9388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_3478_length_9388_cov_6.003429, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000103 DAOQKQ010000000
VERSION     DAOQKQ010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 9388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9388
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3478_length_9388_cov_6.003429"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..657)
                     /gene="hisG"
                     /locus_tag="PKZ49_07900"
     CDS             complement(10..657)
                     /gene="hisG"
                     /locus_tag="PKZ49_07900"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HND36522.1"
                     /translation="MTDITIALSKGRIFEDTAPFLKAAGIIAQDDPETSRKLIIATNK
                     PNVRLIMVRASDVPTYVQYGAADLGVAGKDVLLEHGGMGLYQPLDLQIARCKMMVAVP
                     EDFDYQSAVFQGARLRVATKYLKTARDHFAEKGMHVDLIKLYGSMELAPLVGLADAIV
                     DLVSSGNTLKANHLKAVEEIRPISARLIINQAALKLKRATIQPMLESFESVIGAA"
     gene            complement(671..1309)
                     /locus_tag="PKZ49_07905"
     CDS             complement(671..1309)
                     /locus_tag="PKZ49_07905"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HND36523.1"
                     /translation="MSTNVDNFSKAALSGNTYVLAPLPYADNALEPVISANTLSFHYG
                     KHHKAYVDNLNKLLADTPLAGQSLEQIIKATAGQADKAGIFNNAAQIWNHMFYWHSLS
                     PKGGGEPPAALKQKIEAAFGSVDNCKKELANAAVTQFGSGWAWLVQDGDKLAIAKTSN
                     AETPITKNSRPLLTIDVWEHAYYIDFQNRRPDYVNTVLDKLINWEFAAANLS"
     gene            complement(1403..1837)
                     /locus_tag="PKZ49_07910"
     CDS             complement(1403..1837)
                     /locus_tag="PKZ49_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HND36524.1"
                     /translation="MNFQRGQKKEEPEINLVPMIDVLLVILIFLVITTTYSKFSELEI
                     TLPEATAIEVDKNVQRPNTIDVSVSASGGYTINLSPVTFSSIEALRDALKAAAKDNQN
                     PIIIINADAKASHQSVITVMEAARLAGYNQVTFTTEVQPKLQ"
     gene            1937..2428
                     /gene="purE"
                     /locus_tag="PKZ49_07915"
     CDS             1937..2428
                     /gene="purE"
                     /locus_tag="PKZ49_07915"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HND36525.1"
                     /translation="MVNPLVSIVMGSNSDWNVMQLAAQVLKEFSIPHETLIVSAHRTP
                     DRMFEFAESAMERGIQCIIAGAGGAAHLPGMIAAKTIVPVLGVPVTSKQLNGLDSLLS
                     IVQMPKGVPVATFAIGEAGAANAALFAIALLATKDHQLAAALTAFREKQADTVAAMTL
                     PEV"
     gene            2425..3561
                     /locus_tag="PKZ49_07920"
     CDS             2425..3561
                     /locus_tag="PKZ49_07920"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HND36526.1"
                     /translation="MMILPGARLGLLGGGQLGRMFTMAAQTLGYRVTVLDPADDSPAG
                     RIAERHLQADYLDYQALSDLSNSCAAITTEFENVPAAALRYLAQQRIVSPNANSVSIA
                     QDRIHEKQFLSANGFAVAPFAVIQSGDDLASPIYQSLCPGILKVSQLGYDGKGQVRIT
                     HINDLSPAFEKLRQVPCVLEQFLPLACEISVILARGFDGKTVHFPVIENQHQQGILDV
                     SLVPARVSDSIQQQACRIATELATKLDYQGVLCVEFFLLQDGQLLINEIAPRPHNSGH
                     FSIDACITSQFEQQVRTLCQLPLGSTTMKTPAAVMINLLGDLWHSGEPDWNNVLEQPA
                     AKLHLYGKSEARPGRKMGHITVLGDTLEHALDKALTIRATLSER"
     gene            3563..4453
                     /locus_tag="PKZ49_07925"
     CDS             3563..4453
                     /locus_tag="PKZ49_07925"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HND36527.1"
                     /translation="MKNSTALFETTLNSLPLIHRGKVRDIYAVDADNLLIIQTDRLSA
                     FDVILPTPIPGKGKLLTQISAFWFEKLKHIIPNHLTSVLPQTVVSPQECDQVIDRAFV
                     VKRLQPLPIEAIVRGYIIGSGWKDYQNSGAISGIKLPPGLREAEKLTQGAIFTPSTKA
                     QAGEHDENISFAICEQMLGTQLASQVRDISIALYTEAADYALQKGIIIADTKFEFGQD
                     QAGKLYLIDEVLTPDSSRFWPVDQYQPGMSPPSFDKQFVRNWLEQSGWNKKPPAPEIP
                     DEILLQTVNRYQEALIRLTQ"
     gene            complement(4455..5258)
                     /locus_tag="PKZ49_07930"
     CDS             complement(4455..5258)
                     /locus_tag="PKZ49_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HND36528.1"
                     /translation="MEWWLIYLLLGAIVGYIAGLFGIGGGLVIVPVLVYMFTLQAFPA
                     DRVLHFALGTTMATIIFTSLSSLRMHHVHGAVNWLIVRYLSPGIILGTLVGSTLASTL
                     STSVLGVIVVLFIYYAATQMWLKLLPKPEYTLPGKGGLFMAGGIIGSVSSLVAIGGGL
                     LTVPFLSLCKVKLQQAIGTAAAVGFPIALAGTIGYIINGELQTQALPEYSLGYVYLPA
                     LGWIAAASMLTARLGAKTTHRIQTATLKKLFIVLLYCLGTKMLFDILSN"
     gene            complement(5273..5728)
                     /locus_tag="PKZ49_07935"
     CDS             complement(5273..5728)
                     /locus_tag="PKZ49_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HND36529.1"
                     /translation="MRYWLMKSEPSEVSIDDLAAKPDQTVAWDGVRNYQARNFMRNQM
                     QLGDKTFFYHSSCEQPGIVGITTVSKLAYPDATQFNPKSKYYDPKSTPENPRWFNVEV
                     KLVRKTRLISLKELRSYSALANMRILQKGNRLSITPIDPSEWTFILGKI"
     gene            5802..6254
                     /locus_tag="PKZ49_07940"
     CDS             5802..6254
                     /locus_tag="PKZ49_07940"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HND36530.1"
                     /translation="MPKLLFLSLLLFIVSNANSQIYKWIDEKGRVEYSDQPPPAGSPI
                     VPTRVSLQYSSSYSMDDENTPAPEHVTQKEEGFKERRAAREETKKKQLAEANTKKEQC
                     NQARSNMEILKNTPQLSIPDGMGGVKEADDNFRLKQMEDVKKMIASLC"
     gene            6521..>9388
                     /locus_tag="PKZ49_07945"
     CDS             6521..>9388
                     /locus_tag="PKZ49_07945"
                     /inference="COORDINATES: protein motif:HMM:NF040127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HND36531.1"
                     /translation="MIHSFKFIKAAQVTFTLLLVFMVLIARNSYGFQSSPIVWTTTGI
                     WHATPEESCHYWASRRDIEYVGIPSSKYAEIKAMWVIPWGWNENNVLVMNIIPAVQYQ
                     CGARVFHYGNPPYQQPYEYDWRFGGAFPVCRVGDSPSVHSDTVCNIPDHIPDRGANLG
                     QPSTCVGNPINTATGNKFAQQIDVVLPTFTFTRFYNSFGFSSNGQIGASWRHNFQSSL
                     FINPTTITATRADGKLIYFRLEGAVWINQQSSGEALAPITDVSGSEGGWLLTTADDHK
                     KYYDTAGKLVAISDRAGRRVSLNYDTHYQLATVTDDVGRTLQFTYDGNNRIKALIDPS
                     GEQYQYHYDNQGNLTRVTYPDNHFISYHYNEPAYTSGANLPFALTGITDENGNRFAIY
                     HYDAQGRAIVTEHTDGADRYHLGYNPDGSTTITDSLNTQRTQTFQTLFGVVKMVSQSQ
                     PAGSGCDASSSDISYDANGNVASRTDFNGNKTCFSYDLDRNLEIARVDGLSTNQSCPT
                     DLVSYNPTTNSNERKILTSWHSSFQLPQVITEPNRETIIDYDDLGNITRYHLRDLSSP
                     ASRTWTINYHYHPTVPGFILQTLIDGPRTDLSDITTIDFYPPDANCNGGHYGCRGLTQ
                     RITNALGHQTQMTRYNAHGQIEEMIDPNGLVTTLMYDARQRLLNRVVGTEITNYAYDD
                     AGQLIKVILPDGSSLRYTYDAAHRLIGINDNLGNRIHYTLDSLGNPIREEIFDPLNTL
                     TQTHRSEYDALGRLWKSIGAQNQITEWGYDPNGNFKQSINPLAFTTTNRFDALNRLTQ
                     MTDPAQGLTRLQYNHQDEITQVTAPNGVVTSYTYNGLGDLTREMSADRGLIKYTYDSA
                     GNLKTVTDARGVKHTYFWDALNRPLKRSHSAVTGIPGTTIPTWQYDAGTHGTGRLTSI
                     TDGSGNTQFSYDQQGRLVSKAQTTKLGTVSITHTLGYTYDQT"
BASE COUNT         2676 a         2066 c         2048 g         2598 t
ORIGIN      
        1 ctgaagcgat taagcggcgc cgataacact ttcaaaggat tcgagcatcg gttgaatggt
       61 tgcgcgtttc aacttgagtg cagcttgatt gatgattagc cgcgcggaaa tcggtcggat
      121 ttcttcaacc gctttgaggt gatttgcttt caaggtattg ccgctggaaa ccaaatcgac
      181 gatagcatcc gctagcccaa cgagaggtgc taattccatc gatccatata atttaattag
      241 atcgacatgc atgcctttct cggcaaaatg gtcacgcgca gttttgagat acttggttgc
      301 aactcgtaaa cgcgcaccct gaaacacagc cgactgataa tcaaaatctt ccggaaccgc
      361 aaccatcatt ttgcaacgtg caatttgcaa atcgagcggt tgatacaatc ccataccccc
      421 gtgctcaagc aatacatctt tgccagccac gccgagatca gccgcaccat attgcacata
      481 agttggaaca tccgaggcac gcaccatgat gaggcgaaca ttgggtttgt tggttgcgat
      541 gatgagtttg cgcgaagttt caggatcatc ttgtgcgatg atacccgctg ctttcagaaa
      601 gggcgcagtg tcttcgaata tgcgcccttt tgaaagcgcg atggtgatat cagtcatata
      661 aaataaagga ttaactcaga tttgcagcag caaattccca gttgatcagt ttatccaaca
      721 cggtattcac ataatccgga cgacgattct ggaaatcgat gtagtaagca tgttcccata
      781 cgtcgatcgt gagaagtggg cgggaattct tagtaattgg cgtttccgca ttactggttt
      841 tagcaatggc gagcttatcg ccatcttgaa ctaaccaagc ccatccactt ccaaattggg
      901 tgacagcggc gtttgccaat tcttttttgc aattatcgac actgccaaac gcagcttcga
      961 ttttttgctt cagtgccgca ggtggttcac cgcccccttt aggactcaaa ctgtgccagt
     1021 aaaacatgtg gttccagatt tgagccgcat tattgaaaat ccctgcttta tccgcttgac
     1081 ccgctgtagc tttgatgatt tgttccagtg attgacctgc taaaggcgta tcagccaaga
     1141 gtttgtttaa attatccaca taggctttgt ggtgcttacc ataatgaaag ctcaaggtat
     1201 tcgcagaaat aacaggctct aacgcattat ctgcataagg cagtggtgct aaaacatagg
     1261 tattaccgga caaggcagct ttagaaaagt tatcaacatt cgtactcatg ttttttcctt
     1321 taaataagaa attgaataat gaagccattg tgtttatttt aattatgatt agttaataaa
     1381 ttcttgcgag ccgtatcata acttactgca atttgggttg cacttctgtc gtaaaggtga
     1441 cttgattata gcccgcaagg cgtgctgctt ccatcaccgt gatcaccgat tgatgcgaag
     1501 ctttagcatc cgcattgatg atgatgatcg gattttgatt atccttggct gcagctttta
     1561 atgcatcacg taacgcttca atacttgaaa acgtgaccgg tgacagattg atcgtataac
     1621 cgccactcgc actgacactg acatcaatgg tattggggcg ttgaacattc ttatccactt
     1681 caatcgccgt tgcttcaggt aatgtaattt caagctctga aaattttgaa taagtcgtgg
     1741 taataactaa gaagatcagg atgactaaaa gtacatcgat catcggaacc aaattgattt
     1801 cgggttcttc ttttttctgt ccgcgctgaa aattcatgga agcaatggct attttactga
     1861 tttatcgatt cgttaattct agtttattat taccgctttc gtcaccctca ttaaatttta
     1921 tatttttcca atccaaatgg ttaacccatt agttagcatc gtcatgggca gcaacagtga
     1981 ctggaatgtc atgcaactcg ctgcacaggt gttgaaagaa ttttcaattc cacatgaaac
     2041 actcattgtc tctgcccatc gcacgcccga tcggatgttt gagtttgcag agtctgcgat
     2101 ggaacggggg attcaatgta ttattgccgg tgctggggga gctgcgcatt tgcccggtat
     2161 gattgcggct aaaactatcg tgcccgtatt aggggttcct gtgacttcga agcagttaaa
     2221 tgggctcgac tccctgcttt ccattgtgca aatgccaaag ggtgtaccgg ttgcaacctt
     2281 cgcgattggt gaagccggtg cagccaatgc tgcattgttt gccattgcgt tattagcgac
     2341 caaagatcat caattagctg cagcactcac cgcattccga gaaaaacaag cggacactgt
     2401 agctgcgatg acactgcccg aagtatgatg attttacccg gtgcaagact tggcctactg
     2461 gggggtggcc agttaggtcg tatgtttacg atggcagccc aaacactggg ctatcgggtt
     2521 accgtgttag atcctgcaga cgatagtccg gcggggagaa tcgctgaacg tcatttgcaa
     2581 gcagattatc tcgattatca agcactcagt gacttgagta acagttgtgc tgccattacc
     2641 actgaatttg agaacgtacc cgcagcagca ttgcgctatc tcgcgcagca acgcatcgtt
     2701 agtccgaatg ctaatagtgt ctcgattgca caagatcgta ttcatgaaaa acagttttta
     2761 tcggcaaatg gatttgcagt tgcgccgttt gctgtcatcc aatctggaga tgatttagcg
     2821 tcgccgattt atcaatcgtt atgtcctggt attttgaaag tcagtcaact cggttacgat
     2881 ggtaaaggac aagttagaat tacccatatc aatgatttgt cgccggcctt tgagaaactt
     2941 cggcaagtcc cttgtgtatt agagcaattc cttccacttg cttgcgaaat ttcggttatc
     3001 ttggcacggg gattcgatgg caagacggtt catttcccag ttatcgagaa tcagcatcag
     3061 cagggtatac tcgatgttag cttggtgccc gcccgtgttt cagattcgat tcaacagcaa
     3121 gcctgtcgca ttgctactga gctagcgacc aaactggatt atcaaggcgt gctgtgcgtt
     3181 gaatttttct tattgcaaga tggtcagttg ctcattaacg agattgcgcc tcgcccccat
     3241 aatagtggtc atttttcaat cgacgcttgc atcacttctc aatttgaaca acaagttcgc
     3301 acactgtgcc agttaccgct cggcagtact acaatgaaaa cacccgctgc tgttatgata
     3361 aatttactgg gagatttatg gcatagcggt gagcctgatt ggaacaatgt tttagaacaa
     3421 cctgctgcga aactccattt atatggcaaa agtgaagcga ggccagggag aaaaatgggg
     3481 catattacgg ttcttggtga tacgctcgag catgcattag acaaagcatt aaccatcagg
     3541 gctactttat cggaaaggta agatgaaaaa ttcgacagca ctctttgaga cgactctgaa
     3601 cagccttccc ctcattcatc ggggtaaggt gcgagatatt tatgcggtgg atgcagataa
     3661 cttgttgatt atccaaaccg atcgattgtc cgcattcgat gtgattctac ccacacctat
     3721 tccgggtaaa ggcaaattgc taactcagat ttcagccttc tggtttgaga agctcaagca
     3781 cattattcct aatcatttaa ccagtgtttt gccacaaacg gtggtatctc cgcaagaatg
     3841 tgatcaagtc atcgatcgcg cgttcgtggt aaaacgtctt caaccgcttc cgattgaagc
     3901 aatcgttcgg ggctatatca ttggttctgg ctggaaagat tatcaaaata gtggagcaat
     3961 cagcgggatt aagctaccgc caggattaag agaagctgaa aagctaacgc aaggtgcaat
     4021 ctttacgcct tcaaccaaag cacaggcagg agagcacgat gaaaatattt cgtttgcgat
     4081 ttgtgagcaa atgctgggaa cacagttggc ttcacaggtg agagatatct ccatcgccct
     4141 ttatactgag gctgctgatt atgcgttgca aaaaggaatc atcattgcag ataccaagtt
     4201 tgaatttggg caagatcaag ctggtaagct ttatttgatc gatgaagtat tgactccgga
     4261 ttcatcgcgt ttctggccag tagaccaata tcaaccgggg atgagccccc ccagttttga
     4321 taagcagttc gttcgaaact ggctagagca atcgggatgg aacaaaaagc cccctgcacc
     4381 ggaaattcct gatgagatct tgttgcagac tgttaatcgt tatcaggaag ccttgattcg
     4441 cttaacgcaa taatttaatt ggataagata tcgaacagca tttttgtgcc taaacaatag
     4501 agcaatacga taaatagctt ttttagcgtc gctgtttgga tacgatgtgt ggtttttgcc
     4561 cctaatcgcg ctgttagcat actggctgca gcaatccaac ccaaagctgg taaataaaca
     4621 taccctaagc tatattctgg aagcgcttgg gtttgtagtt caccattgat gatataaccg
     4681 atggttcccg ccagtgcaat cggaaaacca actgctgccg cggttccaat ggcttgctgg
     4741 agtttgactt tgcatagcga aagaaatgga accgttaaaa gcccaccacc gattgccacc
     4801 aaacttgata ccgaaccgat gatacctcct gccatgaata acccaccttt tccgggtagc
     4861 gtatattcag gcttgggtag taacttcaac cacatctggg ttgctgcata ataaataaag
     4921 agtacgacga taacacctag taccgatgtc gataatgtgc tggcaagagt tgatcctacc
     4981 aacgttccca aaataatacc gggtgaaaga tacctgacga tcaaccaatt aacagcacca
     5041 tgcacatggt gcatccgtag gcttgatagc gatgtaaaaa taatcgttgc catagttgtg
     5101 cccaaggcaa aatgtaaaac tcgatcggca ggaaaagctt gcagcgtaaa catataaacc
     5161 agcactggaa cgatgacaag gccaccgcca atgccaaata atccggcgat ataaccaacg
     5221 atagcaccaa gcaataaata aatcagccac cattccatct taattctctg agttaaattt
     5281 tcccaaggat gaatgtccac tcggatggat caatcggcgt aatcgataag cgattgcctt
     5341 tttgcagtat tcgcatatta gcgagcgctg aatagcttct taattctttg agggaaatta
     5401 atcgggtttt tctgacgagc tttacctcaa cattaaacca acgaggattc tcaggtgtcg
     5461 acttgggatc gtaatattta ctcttcgggt tgaattgggt tgcatcaggg taagcgagtt
     5521 tcgacacggt tgtaatacca acaatacctg gctgttcgca gcttgaatgg tagaaaaaag
     5581 ttttatctcc caattgcatt tggttgcgca tgaaattacg tgcttggtaa ttgcgcaccc
     5641 cgtcccaagc aacggtttgg tctggctttg cagccaaatc atcaatacta acttcggaag
     5701 gctctgattt cattagccaa taccgcattt atttatctcc ttctatctcg ataaagttta
     5761 aaagaaaacg atcgatagat ttttatttta ggagaaaaac catgcctaag cttctttttc
     5821 tatcgctatt gctctttatt gtttcaaatg caaatagtca aatctataaa tggatcgatg
     5881 aaaaaggccg cgtggaatac tccgaccaac cacccccagc gggaagtcct atcgtaccca
     5941 cccgcgtcag cctgcaatat tcatcttctt actcaatgga tgatgaaaat accccagccc
     6001 ctgagcatgt aactcaaaaa gaagaaggat ttaaggaacg ccgcgccgcc cgtgaagaaa
     6061 ccaaaaagaa acaattggcc gaggctaaca ctaagaagga acaatgtaat caagcccgta
     6121 gcaatatgga aatcttaaag aatacccctc aactgagtat cccggatgga atggggggtg
     6181 tcaaagaagc tgatgataat tttcgcttaa aacaaatgga agacgtgaaa aaaatgattg
     6241 cgtcgctatg ttaattcgct tcatcaaacc taaaaaaaat acccaccagc acatcgtatt
     6301 taccgagtta atgatgcaga ccctgggtaa tttcccaggg ctttctagta cgttcctgaa
     6361 gttctgactc gtgtgcctat cagaatttat cttttattcg aatcacatca aacgataact
     6421 cagatcgaga aagtaagtag ctgttatttc attatagcta gtgaccttct acttattggt
     6481 agccaattaa cttctcaact aatgcaaaag gaaattagag ttgattcatt cttttaaatt
     6541 tataaaagct gctcaagtta catttacgct gttgttggtg tttatggtgt tgatagcaag
     6601 gaatagttat ggtttccaaa gtagcccgat agtatggact acaacgggga tatggcacgc
     6661 cacccctgaa gaatcatgcc attattgggc atctcgcaga gatattgaat atgtgggtat
     6721 tcctagttcc aagtatgctg agattaaggc tatgtgggta attccctggg ggtggaatga
     6781 aaataatgta ttggtaatga atattatacc tgcagttcag tatcaatgtg gggcacgtgt
     6841 ttttcattat ggaaatcctc cctatcaaca accttatgag tatgattggc ggtttggtgg
     6901 agcatttcca gtttgtagag taggtgattc accttctgtt cacagtgata ctgtttgcaa
     6961 catccccgac cacatcccag atagaggcgc aaacttaggc caaccctcaa catgtgttgg
     7021 taaccccatt aataccgcta caggcaataa attcgcccag caaatcgatg tcgtgctgcc
     7081 aacatttact tttacacgtt tctacaattc gtttggattt tcgagtaatg gtcaaattgg
     7141 tgcctcctgg cgccataact tccaaagtag tttatttatt aacccaacta cgattacagc
     7201 aacccgggca gacggtaaat taatctactt ccgactcgag ggagcggttt ggatcaatca
     7261 gcagtccagt ggagaggcat tagcaccaat cactgatgta agtggctcag agggtggctg
     7321 gttgttaacg actgctgatg atcataaaaa atactacgac actgcaggca agcttgttgc
     7381 aatttcagac cgtgcggggc gacgagtttc tttaaactat gatacgcatt atcaattagc
     7441 taccgtgact gatgacgtag gtcgaacact gcaatttact tatgatggta acaatcgaat
     7501 taaagcattg attgacccat caggagaaca gtatcagtat cattatgata atcaaggcaa
     7561 tctgacgcgt gtcacctacc ctgataatca ttttattagc tatcactaca acgaacccgc
     7621 ttataccagt ggagcaaatt tacctttcgc attaaccggt attaccgatg aaaatggaaa
     7681 tcggttcgca atttatcact atgatgcgca aggaagagcg attgtaactg aacatacgga
     7741 tggcgctgat cggtatcatc tgggctataa cccagatggt agcactacga taacagattc
     7801 cttaaatacg caacgaacac aaacattcca aaccctgttt ggggttgtaa aaatggtgag
     7861 tcaatcacaa ccagcaggct ctggatgtga tgcatcatct agcgatataa gttacgatgc
     7921 aaatggtaac gttgctagcc gtacggattt caatggtaat aaaacttgtt tcagctatga
     7981 tctcgaccga aatctcgaaa tagcgcgtgt tgatgggtta agcacaaatc aatcttgccc
     8041 aacggattta gttagttaca accccacaac caattctaat gaacgaaaaa ttctgacaag
     8101 ttggcactcg agctttcaac tgcctcaagt tattactgaa cctaatcgtg aaacaataat
     8161 tgactatgat gatctaggaa atataacgcg ctatcatttg cgtgatttat caagcccagc
     8221 ttctcgtacc tggactatca attaccacta tcacccaacg gttccaggct ttattctgca
     8281 aactttgatt gatgggcctc gtactgattt atccgatatt acgacaatcg atttttatcc
     8341 tccggatgca aattgtaatg gtggtcatta tggctgccgt ggtctgacac agcgtattac
     8401 caatgcgctg ggccatcaaa ctcagatgac tcgctacaat gcgcatggtc aaatagaaga
     8461 aatgattgac cccaacggat tggtgacaac gttgatgtat gatgcacggc aacgtctatt
     8521 aaaccgcgtt gtaggtactg aaattactaa ttatgcgtat gatgacgcag gacaacttat
     8581 aaaagttatc ctgcctgacg gctcttcgct gcgatatacc tatgatgcag cccatcgtct
     8641 cattgggatc aacgataatc tgggcaatcg aattcattac acgttagaca gcttgggaaa
     8701 tcctattaga gaagagatat ttgatcctct caataccctt acccaaactc atcgcagtga
     8761 atacgacgct ttgggacgat tgtggaaatc aattggcgca caaaaccaaa taaccgagtg
     8821 gggttatgac cctaatggaa acttcaaaca aagtatcaac ccattagcct ttacaacaac
     8881 gaatcgattc gatgcattaa atcgccttac ccaaatgact gatccagccc agggtttgac
     8941 acgtcttcaa tataaccatc aagatgaaat aacccaggta actgctccca acggagttgt
     9001 gactagctat acctacaacg ggttaggaga cctgactcgc gaaatgtcgg ctgatcgtgg
     9061 tttgatcaaa tacacttatg atagtgcagg caatcttaaa actgtaactg atgcgcgggg
     9121 tgttaagcat acctatttct gggatgcatt gaatcgaccg ctcaaacgat cgcattctgc
     9181 ggtaaccggt attcctggga ctaccattcc aacatggcaa tatgacgcgg gcactcatgg
     9241 cacaggacgt cttactagca taaccgatgg ttcaggcaac actcaattta gctatgatca
     9301 acagggtcgt cttgttagca aagcgcaaac gactaagctc ggaacggtga gtataacgca
     9361 tacccttggg tatacttatg atcaaacc
//
LOCUS       DAOQKQ010000104         9381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_3481_length_9381_cov_5.232683, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000104 DAOQKQ010000000
VERSION     DAOQKQ010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 9381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9381
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3481_length_9381_cov_5.232683"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..126)
                     /locus_tag="PKZ49_07950"
     CDS             complement(<1..126)
                     /locus_tag="PKZ49_07950"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase, FolE2/MptA family"
                     /protein_id="HND36532.1"
                     /translation="MNKQIDMPIADVQSSLDTRQIAIDRVGIKAIRHPVRVADKGG"
     gene            complement(286..2130)
                     /gene="dxs"
                     /locus_tag="PKZ49_07955"
     CDS             complement(286..2130)
                     /gene="dxs"
                     /locus_tag="PKZ49_07955"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HND36533.1"
                     /translation="MYPLLDRINSPIELRKLDRKQLPQLADELRQFLIESVASTGGHL
                     SSNLGTIELTIALHYIFNTPYDRVVWDVGHQTYAHKVLTGRRAGMAKLRMKGGVAGFP
                     RRDESEYDAFGTAHSSTSISAALGMAVASQLDDATRRAIAIIGDGAMSAGMAFEALNN
                     AGVMDANLLVILNDNDMSISPPVGALNNYLAKLMSGRFYATARRAGEKVLGVVPPVLE
                     LARRAEEHVKGMMTPGTLFEEFGFNYIGPIDGHDLDVLLTTLSNIKHLDGPQFLHVVT
                     RKGAGYKLAEEDPILYHGVGKFDPHKGIIAKAASKPTYTQVFGDWLCDMAALDTRLIG
                     VTPAMREGSGLVKFSQEYPNRYFDVGIAEQHAVTFAAGAACDGLKPVVAIYSTFLQRA
                     YDQLIHDVAIQNLPVVFAIDRAGLVGADGPTHAGSFDLSYLRCIPNITVMVPADENEC
                     RQMLYTAFQLNTPSAVRYPRGSGIGVKIQKKMNALPIGRGEIRRSGSKIAILAFGTLL
                     TPCLEVGEVLDATVANMRFVKPLDDELVSDLAKTHDLLVTVEENTIMGGAGSAILESL
                     MASSVTVNVLQLGLPDVFLDQGDPVQMLADCGLDKAGILKSIQDRLAK"
     gene            complement(2183..3073)
                     /locus_tag="PKZ49_07960"
     CDS             complement(2183..3073)
                     /locus_tag="PKZ49_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HND36534.1"
                     /translation="MSVNFQEWVKNCQHQLELFLEERLPNNRTAPERLHDAMRYAVLG
                     GGKRVRPLLAFAAGELGGADISRVRVVAAAVELIHAYSLVHDDLPCMDDDVLRRGKPT
                     CHIQYDEATALLVGDSLQSLAFQLVAEYPVTDNPTDQLEMIKQLAQASGSRGMAGGQA
                     IDLASVGKSITLPELESMHIHKTGALIRAAVMLGAHCGNQLSSHDLSRLDHFAKCIGL
                     AFQVVDDILDSEATTATLGKTAGKDAQQNKPTYVSILGVKQARELAESLQQDAYKALS
                     EIKSTQRLLQVTDFIIQRHF"
     gene            complement(3063..3320)
                     /locus_tag="PKZ49_07965"
     CDS             complement(3063..3320)
                     /locus_tag="PKZ49_07965"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HND36535.1"
                     /translation="MKKKTTETLIVEPESFEAASAELESIVIKMESGQMTLEASLAAY
                     KRGMELLQYCQKALQNAQQQIRVLEDGNLRDFTETESINER"
     gene            3771..4250
                     /locus_tag="PKZ49_07970"
     CDS             3771..4250
                     /locus_tag="PKZ49_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36536.1"
                     /translation="MENSLDQIIKYFEEVPLWPFVLLAGLLLFSLGIEFVNRRRRIDA
                     VEYFDSTFLTELAGLYPQATRWPEDLAEHLQESFPVMRDAFENLRGFLPQKQLREYNA
                     AWNNFFDFCRTNGQSHRNVNVKSVSDQPSELNQSAQDIETFHKLVSALLAYSNQFKR"
     gene            4367..5500
                     /locus_tag="PKZ49_07975"
     CDS             4367..5500
                     /locus_tag="PKZ49_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HND36537.1"
                     /translation="MSEIVVRPVDTYQRMGHFIEVPWLVYKDDPNWVPPLRLERRFHY
                     SRFNPFFEHAEWQAWVAYKDNHAVGRISAQIDSLHRQHYGQQTGHFGTLECINDEAVF
                     ATLINSAEQWLIEHDAFNITGPFNLSINQECGVLVEGFDTPPVVMMPHSRKWYDSLLQ
                     QYGYQSARDLLAYWVDVDFTAPKAMQAVNQRYRDQIKLRTLRRKHFNEEMETLRDIFN
                     DAWSENWGFVPFTKAEFSELGNSLRWLVPEDYIQIAELEGRPVAFMAGIPNLNEIFSQ
                     LNGSLFPFGWIRLIKLLKSRAIKTGRIPLMGVRKQYQKTPIGLALAFAVIDAPRQIGI
                     RQGIKAVEMSWILEDNSPMRSILKSIGSKEYKRYRLYEKTLVA"
     gene            5507..6313
                     /locus_tag="PKZ49_07980"
     CDS             5507..6313
                     /locus_tag="PKZ49_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HND36538.1"
                     /translation="MNSNLNYAALVLAADRTANDPIALKAQVSCKAFAPIDGKPMIIR
                     VLDTLIASPTVKSITLCGPPQALLPECPELHERILHGQIGWITNQNSPSLSAAKGLEQ
                     LDPDIPVLLTTADHALLTPEIVEYFLKSASDNEYDATVGAINYEIIHAAFPESKRTVI
                     RLQDGKYCGCNLFAFKPRGRLLVSYWQKAEAQRKRPWKLISQTIGWQAVFKYLLGFLT
                     LNQALSHIEKQIGLSVKIATLPFPEAGVDVDTVSDLMFVEALLKKKAENT"
     gene            6347..7423
                     /gene="lptF"
                     /locus_tag="PKZ49_07985"
     CDS             6347..7423
                     /gene="lptF"
                     /locus_tag="PKZ49_07985"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HND36539.1"
                     /translation="MKIIEKYITRELLIPFFVVIGILIGLFSSFSIARFLTSAVTETL
                     GMIAMLKLVSLRTIIALEVLVPIAFYVAIIYGLSRLNKDQELNIIRSSGFGDNRILFT
                     ILVIALPISIISGVLSLYARPWAYAESYILDAQAEAELNADRFQAGRFYGSEKSGRVI
                     FVQSKDDTGQHMENVFHFVRKGENSEIIVAKQAHQPYQTRLDQRPETTLFEGYIYQLG
                     NNEHKDSAIQFEKLTYYNENESVMNYRRKAASTRTLWDSEQPRDIAELQWRLSRPVAT
                     ILLALIAVSFTRTIPRQSKSDKTFLIAAMVFALYYNLSGLAQTWVEQAVIPEIPGVWW
                     LYSLLLIAVILFLPSIRQKIANLS"
     gene            7420..8481
                     /gene="lptG"
                     /locus_tag="PKZ49_07990"
     CDS             7420..8481
                     /gene="lptG"
                     /locus_tag="PKZ49_07990"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HND36540.1"
                     /translation="MKTAYSYIAKQVIIGMVIATFVLLPLFSFFDLLDQLDDVGKGTY
                     RTADAFLYVAFLLPRRFIQIAPFIALLGTVGALGKLAVNSELVALRIAGLSPLKISLA
                     PVSIGLLLLVLIMILEQFVAPQLHQRAISHRSIALDKSAELGKGLGIWARDEQHILRI
                     GEMLPTQQAIDIEIMYFDHDGYLSTHTYAEFATIFEDYWELNEVTVRTFSANKISSTQ
                     ASSVAWQSFLNADDIGTLTKPPESLSPAELMRHAAFLQSTGQKANAYIMAIWRKIGGG
                     IMIIAMILLAIPFIFGSIREGLGGKLILAAVIGVSVYLLDQIIANAGLLLHMNPVLTS
                     ILPSVALISVAIYFLKRVY"
     gene            8508..>9381
                     /locus_tag="PKZ49_07995"
     CDS             8508..>9381
                     /locus_tag="PKZ49_07995"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HND36541.1"
                     /translation="MSTNTYIYIYEDSEVKLWGLSGRERLERMVQSFQHINLIDDPLK
                     TPDDSPVLLLRADHLFDIRVISTLIHLEGEIVLCNQAGTPVALRTVGNRVPSRIEALR
                     HTTRLSAIHGMKQYSIQDLQVGLQQKLKKKEHPYVLPIAVNQKEALEWELFAGSYKGV
                     TDLVTKWLWPIPAFWVTHLCIRWKITPNQVTVLSIVLALLAGIAFWYGHYASGLIMGW
                     IMTFLDTVDGKLARVTITSSRIGDVMDHGLDLIHPPLWYLAWGIGLTATELPLANLEF
                     LVWLIFIGYIGGRIC"
BASE COUNT         2698 a         2032 c         1880 g         2771 t
ORIGIN      
        1 ccctccttta tccgccactc tgactggatg tcgtatcgct ttgataccaa ctcggtcgat
       61 cgcaatttgg cgtgtatcga gtgaactttg gacatccgca atcggcatat caatctgttt
      121 gttcatgttg attcctctga aaatagtcat tactgcgacg ttgctagaat tcttgtgttt
      181 tttctgaaag gtaaccacat tgctatactc aacataaatc gcgttacaag taatgtgact
      241 accttcaatt taaatggttc ttagagctaa ttcactacaa cggaatcact tagccagcct
      301 atcttggatc gattttagaa tgcccgcctt atctaaccca caatctgcaa gcatctgtac
      361 tgggtcgcct tgatcaagaa aaacatctgg cagccccagt tgtagaacat tcacagtaac
      421 actactcgcc atgagtgatt caagaatagc gcttcctgct ccgcccatga tggtattttc
      481 ttcaacagtc actaataaat catgcgtttt agccaaatca ctcaccagtt catcatccaa
      541 cggttttacg aagcgcatat tggcaaccgt tgcatcaagc acttcgccta cttctaaaca
      601 gggtgtaagt aaagttccaa aagctaaaat cgcaatttta ctacctgagc gacgaatctc
      661 tccgcggcca atcggtaacg cattcatttt tttctggatt ttaacgccga tacctgaacc
      721 acgaggatag cgaacagcag aaggtgtatt gagttggaac gcagtatata acatctgccg
      781 gcactcattt tcgtcagcgg gcaccataac tgtgatatta gggatacatc ttaaatagct
      841 taaatcaaaa cttcccgcat gcgttggtcc atcagcaccg accaacccag ctcgatcaat
      901 cgcaaataca actggcaaat tttgaattgc aacatcgtgt atcagttgat cataggcgcg
      961 ctgtaggaag gtcgagtaaa tcgctaccac gggtttcaat ccatcacaag ctgcgccggc
     1021 tgcaaaggta actgcatgct gttctgcaat accgacatca aaatagcgat tagggtattc
     1081 ttgagaaaat ttgactaaac cggagccctc gcgcattgcg ggggtaacac caattaaacg
     1141 cgtatccagt gcagccatat cacatagcca atctccaaat acttgagtat aagttggttt
     1201 gcttgcggct ttggcaataa tacccttatg aggatcaaac tttccaaccc catgatataa
     1261 aataggatct tcttcggcaa gcttataacc tgcacctttg cgcgtgacga catgtaagaa
     1321 ttgtgggcca tcgagatgct tgatattgct caatgtcgtc aataaaacat cgagatcgtg
     1381 tccatcgatc ggcccaatat agttgaagcc aaactcttca aataaagtac ctggcgtcat
     1441 cattcctttc acatgttctt cagcgcgtct cgcgagctct aaaaccggtg gtaccacccc
     1501 taatactttc tcacccgctc ggcgtgccgt ggcataaaat cgtccagaca tcaatttagc
     1561 caaataatta ttgagtgcgc ctacaggagg cgagatagac atatcgttgt cgttcagtat
     1621 cacaagtaaa ttcgcatcca tcacacccgc attattgagc gcttcaaaag ccatgcctgc
     1681 actcatggca ccatccccaa tgatggcaat ggctcgtcta gtcgcatcat ccagttgaga
     1741 agcaacagcc atgccaagcg cagcgctaat cgacgtactc gaatgggcgg tcccaaatgc
     1801 atcatattcg ctctcgtcac gacgaggaaa accagcgaca cctcctttca tccttaattt
     1861 ggccatccca gcacgacgac cggtaagaac tttatgcgca taagtttgat gaccaacatc
     1921 ccaaaccacg cgatcatagg gtgtgttaaa tatataatgt agtgcgatag tcagttcgat
     1981 tgtgccaaga ttagaagaca agtgtcctcc tgtactggca accgactcaa ttagaaattg
     2041 gcgcaattca tccgcgagct gcggaagctg ctttcgatct aactttctta gctctatagg
     2101 gctattaata cgatctagta atggatacat tcaacacaga ataaaattat tttcaaaaat
     2161 taagaaggta agattgtcaa aactaaaagt gacgttgaat gatgaaatca gttacttgta
     2221 gcaaacgctg ggttgatttt atctcgctga gtgctttgta tgcatcttgt tgtaacgatt
     2281 cagccaattc gcgagcttgc ttaacaccca agatactgac ataagtcggt ttattttgtt
     2341 gagcgtcctt tccagctgtt ttacccagcg ttgccgtcgt tgcttctgaa tcgagaatat
     2401 catctaccac ttgaaaggcc aagccaatac attttgcaaa atgatctaat cgacttaaat
     2461 cgtgtgaaga aagttgatta ccgcaatgcg ctccgagcat cacagccgct cgaattaatg
     2521 ccccggtttt atggatatgc atcgattcaa gttctggcaa ggtaatagat tttccgacac
     2581 tcgctaaatc aatggcctgt ccaccagcca tacctctcga tcctgatgct tgagccaatt
     2641 gcttgatcat ttctaattga tcggtaggat tatcagtgac agggtattct gccaccagct
     2701 gaaaagcgag actttgcaga ctatcgccca ctaaaagtgc ggttgcttca tcatactgaa
     2761 tatggcaagt cggtttaccc cgacgtaaaa catcatcatc catgcaaggt aaatcatcat
     2821 gcacgagaga atacgcatgg atcaattcaa cagccgctgc aacgaccctg acacgagaaa
     2881 tatctgcgcc accgagctca cctgccgcaa aagctagcaa tggtcgaaca cgcttacccc
     2941 cccccaacac agcatagcgc atcgcatcat gtaaacgctc tggcgctgtt ctattgttcg
     3001 gaagccgctc ttctaaaaat agctctaatt gatgctggca atttttaacc cactcctgaa
     3061 aattaacgct cattgatact ttccgtttca gtaaaatcac gtaaattacc gtcttcgagg
     3121 acacgaattt gttgctgtgc attttgcaag gctttttggc aatactgcaa tagctccatt
     3181 cctcgtttat aagctgctaa tgatgcttcc agcgtcattt gaccactctc catctttatc
     3241 acgatacttt ctaattcagc ggatgcagct tcaaagcttt ccggctcaac gattaatgtt
     3301 tcagttgttt tttttttcac gatttttcta aagaaatcat ttgtatactt tgattatatc
     3361 cattgaattc aaatcaatcc ctatgcactg cttcttttaa agccttttaa agcttttgtt
     3421 tctttaatgt taaaaaggtt aaaaatctgg ttattttaac ctgcataatt gggttcatgc
     3481 tattttgatt ctaaatagta tttaaaataa tagtaaatta gcttacttca gataatttgg
     3541 acagaatatt tttataaacc aatggataaa aaacatgcac cgtatcatcc tacaaaatca
     3601 aaactgcttt ctacgctatc tgctttaaaa catattgcta ctcgctatat cctatggcaa
     3661 tatcaagcaa aatcgatatt taatactctc tcaatataaa aattgagcca gtaaaatatt
     3721 acgtcttatc ttgtttataa cctattttta agaatagaat cgaggatatt ttggaaaatt
     3781 cacttgatca aatcattaaa tactttgaag aagtaccact ttggccattt gttctattag
     3841 ctgggcttct tttattctca cttggtattg agtttgtcaa tcgtaggcga cggatagatg
     3901 cggttgaata ttttgatagc acattcctaa cagaattagc tggcttatat ccccaagcta
     3961 cccgatggcc tgaagatctc gctgaacacc ttcaagaaag cttcccagtg atgcgtgatg
     4021 catttgaaaa tttgagagga ttcctacccc agaagcagtt acgtgaatac aatgcagctt
     4081 ggaataattt ttttgatttt tgccgtacca atggccaaag tcaccgtaat gtcaatgtaa
     4141 aatctgtatc ggatcagcca tcagaactta atcaatcagc ccaagatatc gagacatttc
     4201 ataaactagt atctgctttg ctcgcctata gcaaccaatt taagcggtag taaacgatta
     4261 ctctttgcat gcattgcttg cgtttattgg aatctaagct gagttatcac tgtaacttag
     4321 tcttttaaat tgatcatcaa agttttttta tctgataatt aaattgatga gtgagatcgt
     4381 tgttcgtccc gttgatactt atcagcggat gggacatttc attgaggtac cctggttagt
     4441 ttataaagat gatccgaact gggtcccacc ccttcgatta gaaagacgat ttcattactc
     4501 tagatttaat cctttcttcg aacacgctga atggcaagcc tgggtagctt ataaagataa
     4561 tcacgcggta ggaagaatca gtgcgcagat cgattctttg catcggcagc attatgggca
     4621 gcagacaggc catttcggca cactagagtg tattaatgat gaagccgtgt ttgcaacgct
     4681 tataaatagt gctgagcaat ggctaatcga gcatgacgct ttcaatatta cgggtccatt
     4741 caacctatcg atcaatcagg aatgtggcgt attagtggaa ggcttcgata ccccaccggt
     4801 tgttatgatg cctcattcgc gtaaatggta tgactcgttg ctacagcaat atggctatca
     4861 atcagcacgt gatctccttg cttattgggt tgatgtcgat ttcactgcgc ccaaagccat
     4921 gcaagcagtc aaccagcgct atcgcgatca aattaaacta aggacgctca gacgcaagca
     4981 ttttaatgaa gaaatggaga cactaagaga tatctttaat gatgcttggt ccgaaaactg
     5041 gggatttgtt ccttttacca aagctgagtt ctctgaactg ggtaatagcc tacgctggtt
     5101 agttcccgaa gattacatcc aaatcgcaga actagagggc agacccgttg cattcatggc
     5161 aggtattcct aatctcaatg agattttttc acaactcaat ggtagtctct ttcctttcgg
     5221 ttggattcga ttgattaaat tactcaaatc ccgtgccatt aaaacgggcc gtattcctct
     5281 gatgggggta agaaaacaat accaaaaaac accaattggg cttgcccttg cctttgcagt
     5341 aatcgatgcg cctcgacaaa ttggaattag gcaaggtatc aaagcggttg aaatgtcatg
     5401 gatcctcgaa gacaattcac ctatgcgtag tatattaaaa agtattggta gcaaagaata
     5461 taaacgctat cgtttatacg agaaaacgct tgtagcataa catccaatga attcaaacct
     5521 aaattatgct gcgctggtgc ttgctgcaga tcgtaccgca aatgatccca tcgcgctaaa
     5581 agcgcaggta tcatgtaaag cttttgcacc tatcgatgga aaaccaatga taataagagt
     5641 actagatacc cttattgcaa gccctacagt taaatccatt acgctatgtg gaccacctca
     5701 ggccctgcta ccagagtgcc cagaacttca tgaacgcatc ttacatgggc agattggttg
     5761 gattacaaat cagaattcac cgagtttaag cgctgcaaaa gggctggaac aacttgaccc
     5821 agatattccc gtgcttctca ccacggcaga tcatgcttta ctgacccctg aaatcgtcga
     5881 atattttcta aaatcggcca gtgataatga atacgatgca acagttggtg caatcaacta
     5941 tgaaataatt catgcggcat tccctgaaag caaacgaact gttattcgat tacaagatgg
     6001 taaatattgt ggctgtaatt tatttgcatt taaacccaga gggcgattac tggtttccta
     6061 ttggcagaag gcagaagccc aacgaaagcg tccctggaag ctcatatcgc aaaccattgg
     6121 atggcaagct gttttcaaat atctattggg tttcttgaca ttgaatcaag cgctttcgca
     6181 tatcgaaaaa caaattgggt taagcgtaaa aatcgctacg cttccttttc cagaagctgg
     6241 agttgatgta gacacggtta gtgacttgat gtttgttgaa gcgctgttaa agaaaaaagc
     6301 agaaaatact taattaagca ttacacgttt ctctcagagt gaaaagatga aaataataga
     6361 aaaatacatc actcgcgaat tattgattcc ttttttcgtg gtcattggaa ttctaattgg
     6421 actattctct agctttagta tcgcacgttt tctaaccagt gcagtgactg aaacgcttgg
     6481 tatgatcgca atgcttaagc tcgttagcct tagaaccatc attgcgttgg aagtactggt
     6541 tccaatcgcc ttctacgttg ccatcattta tgggttgagt cggttaaata aagatcagga
     6601 attgaatatt attcgttcat ccgggtttgg agacaaccgc attttattta caattttggt
     6661 aattgcttta cctattagta tcattagtgg cgttctctct ctttatgctc gtccatgggc
     6721 atatgcagaa agttatatct tagatgctca ggcagaagca gaattgaatg cagatcgatt
     6781 tcaagctggt cgattctatg gtagtgaaaa aagtgggcgg gttattttcg ttcagtcaaa
     6841 agatgatacc ggtcagcaca tggaaaatgt atttcatttt gtcaggaaag gggaaaatag
     6901 cgaaattatc gttgcaaaac aagcacacca accctatcaa acaagattgg accaaagacc
     6961 tgaaacaacg ttattcgagg gttatatcta ccaactcggc aataacgaac acaaagatag
     7021 tgcgatacag tttgaaaagt taacctacta taatgaaaat gagagtgtaa tgaattatcg
     7081 tcgtaaagct gcatcaacac gaacgctttg ggattcggag caacctcgcg atattgctga
     7141 attacaatgg cgactatcac gtcccgttgc gacaatttta cttgcactca ttgctgtctc
     7201 atttacgcgc acgattcctc gccaaagtaa aagcgataaa actttcttaa ttgctgccat
     7261 ggtttttgcg ctttattata atttaagtgg acttgcacaa acttgggtgg agcaagcggt
     7321 tattcctgaa ataccgggtg tttggtggct ttatagcttg ttgttgattg cagtcatttt
     7381 atttctgccg agtattcggc aaaaaatagc taatctatcg tgaaaactgc atacagctat
     7441 attgcaaagc aagtcattat cggcatggtg attgctactt ttgtattatt accgttattc
     7501 agtttttttg atttattaga tcaacttgac gatgtgggaa agggtacata tcgtacggcc
     7561 gatgcctttc tctatgttgc atttttacta ccgcgcagat ttattcaaat tgcgcccttt
     7621 atcgcattat taggaaccgt cggcgcctta ggtaaactcg ctgtgaattc agagcttgta
     7681 gcgttaagga ttgcgggact atcccctcta aagatcagct tggcaccggt ttctatcgga
     7741 ctactgctac tggttctaat catgatcctt gagcaatttg ttgcaccaca actgcatcaa
     7801 cgagcaattt cgcaccgctc gattgcatta gataaaagtg cagaactagg aaaaggattg
     7861 ggcatctggg ctcgcgatga gcaacatatt cttcggattg gtgaaatgct acccacgcag
     7921 caagcaatcg atatcgaaat tatgtatttt gatcacgatg gatatttatc cactcatacc
     7981 tatgcggaat tcgcaacaat cttcgaagat tattgggaac tcaatgaagt caccgttaga
     8041 acgttctctg caaataaaat ttcttcgacg caagcaagca gtgtagcgtg gcaatcattc
     8101 ttgaatgccg atgatatcgg aaccttaacc aaaccgccag agagtctgtc acccgctgaa
     8161 ttaatgcgac atgcggcgtt tttacaatcg acaggccaaa aagcgaatgc ctacattatg
     8221 gctatctggc gaaaaatcgg cgggggaatt atgattatcg cgatgattct acttgcgata
     8281 ccttttattt ttggctcaat tcgtgaaggg cttggcggta aattaatact ggctgctgtc
     8341 atcggggtca gtgtttattt attagatcag atcattgcca atgcaggact actattgcat
     8401 atgaatccgg tcctgacttc tattttacct agcgttgcac tcatcagtgt tgcgatctat
     8461 ttcttgaagc gagtctatta agttacttaa caaccactta acacgctatg tcaacaaata
     8521 cttatattta tatctacgaa gatagtgaag ttaaattgtg gggactatcc ggtcgtgaac
     8581 gattagaacg catggtgcaa tcattccagc atattaattt gattgatgat ccgcttaaaa
     8641 ctccagatga ttcgcctgtc ctgttattaa gagccgatca tttgtttgat atacgtgtta
     8701 tttctacttt aatccaccta gaaggtgaaa ttgtattatg caatcaagca ggaacccctg
     8761 ttgcgttacg aacggttgga aatcgggttc ctagccgaat tgaggcgtta cgccacacaa
     8821 cacgcctttc tgcaattcat ggaatgaagc aatattccat tcaagatctg caagtcggcc
     8881 ttcaacaaaa attaaagaaa aaagagcacc cttatgtcct accaattgca gtcaatcaaa
     8941 aggaagcgct tgaatgggag ctttttgctg gctcttataa aggagtcacc gatctcgtca
     9001 caaaatggtt atggcctatc cctgctttct gggttactca tttatgcatt cgctggaaaa
     9061 taactcctaa ccaagttacc gtattaagta ttgtacttgc tttactagca ggcattgctt
     9121 tttggtatgg tcattatgcg agtggcttga ttatgggatg gatcatgact ttcttagata
     9181 cagtcgatgg aaagttagca cgagtcacga ttacgtcgag tcgaatcggc gatgttatgg
     9241 atcatggctt ggatctcatt caccctccct tatggtatct agcttggggc atcggtttaa
     9301 cggctaccga gctgccatta gctaacctag aatttcttgt ttggttaata ttcattggct
     9361 atatcggtgg gcgtatttgc g
//
LOCUS       DAOQKQ010000105         9318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_3522_length_9318_cov_6.063586, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000105 DAOQKQ010000000
VERSION     DAOQKQ010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 9318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9318
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3522_length_9318_cov_6.063586"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /locus_tag="PKZ49_08000"
     CDS             <1..1093
                     /locus_tag="PKZ49_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36542.1"
                     /translation="GELGTAKIASEDVDAVVNYFTYLLTKGSTQIEITDRQMLFRLSI
                     PLSMGITTSYLNLETNFIEVEGFPSLQSIKISYFQLPVFLITKIFPKLAEWSISIIPE
                     IQLAVSALQQVRMSPSGIDIVYRWQGGLFDKLNKPFAMSLPLVNKQELDRFYRYHFIL
                     TNFTASKSSPIPLSQLLIPVMRLASEQSTKGNAIEENRAAILITSLHILGISLKTLFP
                     EAINWPKQQKYRVTLDGREDFAKHFILSAAITAYADTKLSDAIGLYKEIEDARSGSGF
                     SFNDIAADRAGTKFGEKAVENSMSALKIQEKISSGLDDKDLMPAWSDLPEFLTEAVFK
                     ERFGDINTPSYLQIMDKIEQRIANLIIFH"
     gene            complement(1097..2812)
                     /locus_tag="PKZ49_08005"
     CDS             complement(1097..2812)
                     /locus_tag="PKZ49_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HND36543.1"
                     /translation="MKSKTFLFLLLIGLAGCNQLPKVGSADSARLAKDSQSQIDSPKQ
                     ELTSELLFDYLLAETALQRENIDVAIQSYSRLAKNTQDPRIAERATDIALRARRPDAA
                     QEAVARWVNLEPNSMNARQAAAALFVSMGKLDKAKPHLEKLLSSEKSSVDKAFMHLNK
                     LLSRHPQKAETYKLVQSLAAGYPNLPESHFAVSQAAWSNNQLDIALKEMKRALELRPD
                     WEIAAIHQGRILQQIANAEAMTFYENYLGKYPKANDMRIAYVRMLMTEKNFPLAREHF
                     QKMMAENPVNPDIAVAVGLLSLELNDFEGAEENFKKALELGYEDNNNIYFNLARIYEV
                     TQRTDDALETYQRISGGERFFPAQIRYAALIAKKEGINRARQHLHQIPTETDQQRIQL
                     MLAEAQLLREVGKHQDAFNLLDKNLARNPDQPELLYDRALAADKIDRFDIVEKDLRRL
                     IELKPDNAHAYNALGYSLAERGIRLAEALSLIQKAVELSPDDPYIMDSLGWVYFRMGK
                     IQEGLNYLNLAFSSRPDPEIAAHLGEVLWVQGERENAEKIWRSALETDPDNETLLDTM
                     KRLMQ"
     gene            complement(2968..3654)
                     /locus_tag="PKZ49_08010"
     CDS             complement(2968..3654)
                     /locus_tag="PKZ49_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HND36544.1"
                     /translation="MEHSTISGNSVPTFSRQHQGYIYLWTLFSISLAGVALAGLGPMW
                     QTAAQREKEIELLYVGDQFRLAIEAFYNNATSANRSQRYPESLSDLLKDEGSIVTKRY
                     LRKIYMDPMTNSFEWGLIKQGEKGITGVYSLSTKIPIKRANFPEQYAAFSKAKSYQDW
                     KFNHASGTTGQGRQQTQPNAPPGSPGQSQNTNPFTGQSTPSGQNRGGPANPFPQNQPD
                     NSRANPFSTQ"
     gene            complement(3629..4003)
                     /locus_tag="PKZ49_08015"
     CDS             complement(3629..4003)
                     /locus_tag="PKZ49_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HND36545.1"
                     /translation="MQRPNGFTLIELLVVMAVIATLLSIVAPRYFNSIDRSREAVLKQ
                     NLSIMRDAIDKFYSDTGKYPLSLNQLVEERYIRAVPIDPMTESSQTWIEVPPPDPEIE
                     GMYDVRSSSDRQALDGTFYDKW"
     gene            complement(4018..4515)
                     /locus_tag="PKZ49_08020"
     CDS             complement(4018..4515)
                     /locus_tag="PKZ49_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HND36546.1"
                     /translation="MPFLQKAFCRPNQGFTLVEMIVVVALLSILASAAMPFYQMTVKR
                     EKEQELRIALRQIRTAIDLYKQAADEGRVEKKADEVGYPKRLEMLVEGVPNIKDPKKN
                     MIYFMRKLPRDPMFDNPDIPNADTWGKRSYESPPDDPDEGDDVFDVYSRSNKVGLNGI
                     PYNRW"
     gene            complement(4524..6785)
                     /locus_tag="PKZ49_08025"
     CDS             complement(4524..6785)
                     /locus_tag="PKZ49_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin and TonB N-terminal domain-containing
                     protein"
                     /protein_id="HND36547.1"
                     /translation="MRLIGYLLSIVLVLSGCATFTNRNEAFLEGKQLIAQGQLDAGLK
                     KLEQAAYEEPGSKEIRAVLFRERETIINKTLFEADKLLASDNLDAAEKKYRRVLEIKP
                     NEERARDGLDALNLSRYQSATINRAKEMLDRNDVEGAEAAVRAVLQENPYHEEARLLV
                     KRLNEHIARAEEPVLALVTAFNNPITMEFRDTALKTIFELISKTAGINFIFDKTVQQD
                     SKTSIFVRNNRIEDVLKLLLMTNQLAYKILNDNTLLIYPDTPAKQKDYQELVVRSFHV
                     ANTDVKQMVAMIRGLVKAKDIYVNEKLNLFVIRDTLEAIRLVERLVAINDFPDPEVML
                     DIEVLELSRTRVMELGPKLPQSVTFSGVPGVGDATVAGVAKFSAIGAAQSTSDGLRNF
                     TVNNQVIIDFKKTLTVDDILANPRIRVKNREKAKILLGDKIPIFQASATATGVISQNV
                     TYLDVGLKVEVEPIISFNNEVSVKVALEVSTLGASESSGTTRAFRIGTRSAETLLSSK
                     DGETQVLAGLIRDNDRKGFAGLAGLIDLPVIGRLFTSHSFNRESTEIILLITPYVMRN
                     ITQPTKLESEFYSGTAAAAGKLPATIRKTAPKSLAITPAGAGSASAAFSAPMLSDDTE
                     AIPNPFAAAAQANPATPTLSLQAPATIAMDKEFTATIRLMTQNTKLASELELVYDKGM
                     LEMLDGGEKSGSRTLKLGREEPNGMTAVLRFKAVSPNPGSTNITVQSISTRNEKGESV
                     DVDLPSPAIIEIQ"
     gene            complement(6785..7402)
                     /locus_tag="PKZ49_08030"
     CDS             complement(6785..7402)
                     /locus_tag="PKZ49_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36548.1"
                     /translation="MKSLALEPTALRKSILGIALLLTIAAAIWVEQEDQDAIDEIQPA
                     LSVKDSSKPSMQRKEASSLAIHPLGQRKFNTEADDIFAATSWEPTRTPRGGDRSLFGG
                     TASSEDDEGDPFTAMMKHAKSKPQPAPAPVAPPLQFKYLGKVIEGKTTRVFLALADKN
                     HVVGIGERIDNQYRIERISDETIEFTYLPLRIKQTLLINQQSGKL"
     gene            complement(7405..7959)
                     /locus_tag="PKZ49_08035"
     CDS             complement(7405..7959)
                     /locus_tag="PKZ49_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36549.1"
                     /translation="MDRYALNNLLSEIRWQTLRLSAVGKLGFGLMVLSVVFFLAAVWP
                     QYETLDQAQAKVTLLQQQSQRDASQPIQLDDNQAIQVFYDSFPNVDSSPYWVKEIDAV
                     AKKRGVELHGSDYRLVSDKGSRLARYEMILPVRGSYPQIRAFIADMLVTIPAVALVDM
                     IIKREDIKSGRLEVRLSLNLYLNQ"
     gene            complement(7941..8477)
                     /locus_tag="PKZ49_08040"
     CDS             complement(7941..8477)
                     /locus_tag="PKZ49_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HND36550.1"
                     /translation="MRRLSLKFPYTGQQTSPLDYGLLLLGLVFLVATVLHLKSIMEEI
                     SYWETRADRFENAQQQKRAPKTSSSRTNQESQQELKQAREIMKQLNLPWEVLFDSLEY
                     TVTKDIALLSVQPSVANQSIRINGEARNLAALTEFVEAMEREAFFENAHILSYKVKQE
                     NPHRPILFSLITSWIDTP"
     gene            complement(8464..>9318)
                     /locus_tag="PKZ49_08045"
     CDS             complement(8464..>9318)
                     /locus_tag="PKZ49_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36551.1"
                     /translation="VSPSWRDQIHIFFASGRIDLVRIPKGLKALPQPKISINFEANPD
                     NSLWKIAIGQLTQLLEKENSVSSLKGAELSITLSNHFVRYAVVQFQQGIKDAHEFFAY
                     AQFQMREIYGDRIDSWDISMSDWDPKLGGICAALARDLVQQLQALTVRFHMKLKQIEP
                     YLTAAFDQWSRAINYQRFWFIVVEPGRLCALLFSNGGWKSIRNQRLLNSVETELLVLL
                     EQEAIIATQREPVELAFIYAPEHTNLQLPLDKGWQFNRIPFDGILAPNHFPAPVGNTS
                     ELPAHAST"
BASE COUNT         2510 a         1963 c         1948 g         2897 t
ORIGIN      
        1 tggggagtta ggcacggcaa aaattgcttc agaagatgtt gatgcggtgg taaattactt
       61 tacctatctt ttgaccaaag gtagtactca aatcgaaatc actgaccgcc aaatgctgtt
      121 tcgtttaagt attcctttat cgatgggcat cacaaccagt tatttaaact tagagacaaa
      181 ttttattgaa gtagaaggtt tcccttctct tcagtcaata aaaatcagtt attttcaact
      241 acctgttttt ctaatcacaa agatttttcc gaagctagca gaatggtcga tttctattat
      301 tcctgaaata caattggcag ttagtgcgct acaacaagtt agaatgtctc cttctggaat
      361 tgatatcgtc tatcgttggc aaggaggttt attcgataaa ttgaataaac ccttcgcaat
      421 gagcctccct ctagtcaata aacaagaact cgatcgtttt tatcgatacc actttatcct
      481 tactaatttt actgcgagta aaagtagccc gattccgtta tcgcaactat taattccagt
      541 catgcgcctt gcttcagagc agtcaactaa aggaaatgca atagaagaaa atcgggcagc
      601 aattttgatt acctcacttc atatattggg tatatcacta aaaacacttt ttccagaagc
      661 aattaattgg cctaagcagc aaaaatatcg ggttacactg gatggacgcg aagatttcgc
      721 aaagcatttt atactttctg ctgcaattac agcttacgcg gacaccaagt tatctgatgc
      781 gatcggactt tataaagaaa ttgaagatgc tcgatcaggg agtggatttt cttttaacga
      841 tattgctgct gatcgtgccg ggacaaaatt cggtgaaaaa gcagttgaga attctatgtc
      901 agcactgaag atacaagaaa aaatatcgtc tgggcttgat gataaggatt tgatgcctgc
      961 atggtcagat ttgcctgagt tcttaaccga agcagtattt aaagaacgat tcggtgatat
     1021 taatacgcct tcttatttac agataatgga caaaatcgag cagcgtattg caaatttaat
     1081 tatttttcac tagcagctat tgcattaacc gtttcatggt atcgagcaaa gtttcgttat
     1141 ctggatctgt ttcaagtgca gaacgccaaa ttttttctgc attttctctt tctccttgaa
     1201 cccacaatac ttcacctaaa tgcgctgcaa tctctggatc aggtcgagaa ctaaatgcaa
     1261 ggttaagata atttaagcct tcctggattt ttcccattcg aaagtaaacc caacccagac
     1321 tatccataat atagggatca tctggggata attctactgc tttttggatt aacgataatg
     1381 cttctgctag gcgaatgccg cgctcagcca aactatatcc taacgcatta taggcgtgtg
     1441 cattatcagg tttgagttca attaatcgcc gtaaatcttt ttcaacaatg tcaaatcgat
     1501 caatcttatc tgcagcgagt gctctgtcat agagtaattc gggttggtct ggatttctag
     1561 ccaggttttt gtctaataga ttaaacgcat cttgatgttt acctacttct ctcagcagtt
     1621 gtgcttcagc aagcattaac tgaattcgtt gctggtcagt ttcagtaggt atttgatgga
     1681 gatgctggcg tgcacggttt attccttcct ttttagcaat taaagccgcg tatcggattt
     1741 gagctgggaa aaaacgctct ccacctgaaa tgcgttggta ggtttcaagt gcatcatcgg
     1801 ttctttgcgt tacttcgtag attctagcta gattaaaata gatattgtta ttatcttcat
     1861 aaccaagctc aagcgccttt ttgaaatttt cttccgcgcc ttcaaaatca tttaattcta
     1921 gtgacaataa accaactgcc actgcaatat caggattgac agggttttca gccatcattt
     1981 tctgaaaatg ctcgcgggct aatggaaaat tcttttcagt cattagcatt cgcacatagg
     2041 cgattcgcat gtcattggct ttaggatact taccaagata gttctcataa aaagtcatgg
     2101 cttcggcatt tgcaatttgt tgcaaaatac ggccctggtg gatagcagcg atttcccaat
     2161 cagggcgtag ttctaacgca cgtttcattt ctttgagcgc tatatcaagc tgattatttg
     2221 accaagcggc ttgcgataca gcaaaatgag attcgggtaa gttgggatag cctgctgcga
     2281 gcgattgaac gagcttatag gtttctgcct tttggggatg tcgtgataat agcttattta
     2341 gatgcatgaa cgctttatcc accgagctct tttctgacga tagcaatttc tcgagatgcg
     2401 gttttgcttt gtcgagttta cccatactga caaacaatgc cgctgctgct tgtcgggcat
     2461 tcattgagtt gggttctaaa ttaacccagc gtgctacagc ttcttgggca gcgtcaggac
     2521 gacgtgcacg tagcgcaata tcagttgccc tttcagcgat tcggggatcc tgtgtgtttt
     2581 tagctaaacg agaatagctt tgtatagcaa catcaatatt ttctcgttgt agcgctgttt
     2641 cagccaataa gtaatcaaaa agtaattcgc ttgttagctc ttgcttcggt gagtcaattt
     2701 gagattggct atcttttgcc aatcttgcac tatcggcact cccaacttta ggtaattggt
     2761 tacaacctgc aagtccaata aggagtaaga atagaaacgt tttagatttc atttaaatta
     2821 aacgcataaa taataatagg actgataact aaataatcag ttctttaaac tacgacatat
     2881 ttttcaattt acatgacgca agtttataac gaacactcat gaataatcat cttatttttg
     2941 tatttttaat aaaaatcaaa aaataaatca ttgagttgag aaaggattgg ctctgctatt
     3001 atcgggttga ttttgtggaa aaggatttgc tggcccacct ctattttgtc cggaaggggt
     3061 tgattgacca gtaaatggat tggtattctg actttgtccg ggagatccgg ggggggcatt
     3121 gggttgagtt tgttgtcgtc cctgacccgt tgttccagat gcgtgattaa acttccaatc
     3181 ttggtaactc ttagcttttg agaatgctgc gtattgttca ggaaaattag cgcgtttaat
     3241 gggtatttta gttgataagc tgtaaacacc ggtaatacct ttctcaccct gtttaataag
     3301 gccccattca aagctatttg tcatgggatc catataaatt ttccttagat accgttttgt
     3361 aacgatagac ccttcatctt ttaataaatc agataatgat tcggggtagc gttgacttcg
     3421 attagcgctg gtagcgttat tataaaaagc ttcaattgct aggcgaaatt gatcacccac
     3481 ataaagaagt tcgatttctt tttctctttg agcagcggtt tgccacattg gccctaatcc
     3541 tgctaatgca acacccgcga gcgaaattga aaaaagtgtc cataaataaa tgtagccttg
     3601 atgctgacga gaaaaggtgg gcacactatt accacttatc gtagaatgtt ccatctaaag
     3661 cttggcgatc tgagctactg cggacatcat acatgccttc aatttcagga tcagggggag
     3721 gcacctcaat ccaagtttgt gaactttcgg tcattggatc gatgggaact gcgcgaatgt
     3781 agcgttcttc aactaattga ttgaggctta atgggtattt cccggtatcg gaataaaatt
     3841 tatcgatcgc atcgcgcata atacttagat tctgcttaag aacggcttct cgtgaacgat
     3901 cgatcgaatt gaaatatctg ggagcgacaa tgctcaataa agtagcgatg acggccatga
     3961 cgaccagtaa ctcaatcaat gtaaaaccat tgggtcgctg catgctagtt ttccttatta
     4021 ccatcgattg tagggtattc cattcaatcc aactttatta gaccgggagt aaacatcaaa
     4081 tacatcatca ccttcatctg gatcgtcagg ggggctttca taactgcgtt taccccaagt
     4141 gtcagcattt gggatatctg gattatcaaa catcgggtca cgtggtaact tacgcatgaa
     4201 gtaaatcata tttttcttag ggtctttaat attaggaacg ccttcgacaa gcatctctag
     4261 ccgtttcggg taccctactt catccgcttt tttctctact ctaccttcat cggctgcttg
     4321 cttgtacaaa tcgatggcag ttcgaatttg ccttaaagca atacgtaatt cttgttcttt
     4381 ctcccgtttc acggtcattt ggtaaaaagg cattgcagcg gaagctagga tagagagtaa
     4441 agcaacgacc actatcattt cgactagcgt aaacccttgg tttggtcgac aaaaagcttt
     4501 ttgtaaaaaa ggcatcatgc ctgctactga atttcaataa ttgcaggtga tggaagatct
     4561 acatcgactg attctccttt ctcattgcgg gttgaaatgc tttgtaccgt gatatttgtc
     4621 gagccaggat ttggactgac cgctttaaat cgcaagaccg cagtcattcc attcggttct
     4681 tctctaccta atttcagtgt gcgcgatcca gatttttctc caccatccag catctctagc
     4741 atccctttat cgtagaccag ttcaagttca ctcgctagtt tagtattttg cgtcattagg
     4801 cgaatcgtcg ctgtaaactc tttgtccatt gcaattgtcg cgggagcttg taggctcaac
     4861 gttggtgtgg ctgggttggc ttgtgccgct gctgcaaatg gattaggaat tgcttcggta
     4921 tcgtcagaca gcatcggtgc actgaaagca gctgaagctg agcctgctcc cgcaggagtt
     4981 attgctagcg atttgggcgc tgtttttcgt atcgtggctg gcaattttcc tgctgccgct
     5041 gccgtacctg agtaaaactc gctttcaagt ttggtgggtt gggtgatatt acgcatcaca
     5101 tacggggtaa tgagcaagat gatttccgta ctttctcgat tgaagctatg actcgtaaag
     5161 agtcttccta tgacaggtaa atcgattagc cctgcgagac ctgcaaaccc ttttcgatca
     5221 ttatcgcgta tcaatcccgc taacacttga gtttcaccgt cttttgatga taataacgtt
     5281 tccgcactgc gcgtccctat tctaaacgct ctggttgttc cagacgattc agaggcaccc
     5341 aaggtactga cttccaaggc gactttgact gaaacctcat tattaaatga aatgatcggc
     5401 tcaacttcga cttttaatcc tacatccaga taagttacat tctgagaaat gacacccgtt
     5461 gcggtcgcgc tggcttggaa aattggaatt ttatccccta gcaggatttt ggctttctca
     5521 cgatttttca cgcgaattcg aggattagcc agaatatcat cgacagttag agtcttttta
     5581 aagtctataa tgacttggtt gtttactgta aaatttctaa gcccgtcgct tgtcgactgt
     5641 gcagcaccaa tggcactaaa ttttgcaaca cctgctacag tggcatcacc gacgccaggt
     5701 acgccgctaa acgtaactga ttgaggtagt ttaggtccca attccatgac gcgagtacga
     5761 ctaagctcta gtacttcaat atcgagcatc acttcgggat cagggaaatc attgattgca
     5821 acgagtcgtt ctacgaggcg aatcgcttct aacgtatctc gtattacaaa aagattgagt
     5881 ttttcgttga catagatatc ttttgcttta actaaccctc ggatcatcgc gaccatttgc
     5941 tttacatcgg tattggcaac atgaaagctg cgaacaacta attcttgata atctttctgc
     6001 ttggccggtg tatcggggta aatcaacagc gtattatcgt tcaatatttt ataagcaagc
     6061 tgattggtca tcagcaatag cttcaataca tcttcaattc gattatttct aacaaaaatt
     6121 gaggtttttg agtcttgttg taccgtttta tcaaaaataa aattgattcc agccgtcttt
     6181 gaaatcagtt caaaaattgt tttaagcgct gtatcgcgga actccatagt gattggatta
     6241 ttaaatgctg tgactagggc aagaacaggc tcttctgcgc gcgcaatatg ttcattaagt
     6301 cgcttaacga gcagacgggc ttcttcatgg taggggtttt cctgcaatac agccctaacc
     6361 gcagcttctg caccttctac atcattgcga tccagcattt cttttgctcg attgatggta
     6421 gctgattgat aacgtgaaag attcagcgca tcgagtccat ctcgtgcacg ttcttcattg
     6481 ggtttgattt ctaacaccct acgatatttt ttttctgctg catctaagtt atccgacgcg
     6541 agtaatttgt ccgcctcaaa cagggtttta ttgatgatcg tttcacgttc acggaaaaga
     6601 acagctctga tttccttact accgggttct tcatatgcgg cttgttctaa tttttttaat
     6661 ccggcatcta actgaccttg agcgattaac tgctttcctt ctaagaaagc ttcattgcga
     6721 ttagtaaaag ttgcacaacc gcttaatact aaaacgatac tcaataaata cccaattaat
     6781 ctcattaaag ctttccagat tgttgattga tcaaaagcgt ttgtttaatg cgcagcggca
     6841 aataagtaaa ttcaatcgtt tcatcgctaa ttcgctcgat acgatactga ttgtcgatac
     6901 gttcgccaat tcctactaca tggttcttat ctgctagtgc tagaaacaca cgggttgttt
     6961 ttccctcgat gacttttcca agatatttga attggagtgg cggcgcaacg ggtgcaggcg
     7021 ctggctgagg ctttgatttt gcatgtttca tcatcgctgt gaagggatct ccctcatcat
     7081 cttcggatga tgccgtgcca ccaaataagg aacgatcacc accgcgtggg gttcttgtcg
     7141 gttcccaaga cgtggctgca aaaatatcgt ctgcctctgt attaaatttt ctctgaccca
     7201 gtgggtgaat ggctagggag ctagcttctt ttctttgcat tgatggtttt gatgaatctt
     7261 ttaccgataa agccggttga atctcatcaa tggcgtcttg gtcttcctgc tcaacccaaa
     7321 ttgccgccgc tatcgttaga agaagtgcaa tacctaatat cgattttcgg agagccgtcg
     7381 gttctaacgc taggcttttc atgattattg attgagatac agatttaggc taagtcgtac
     7441 ttctaatcga cccgatttga tatcttcgcg ttttatgatc atatcaacta aagcaacagc
     7501 aggtatggtt actaacatat ctgcaataaa tgcacgaatt tgcggatagc tgccgcgcac
     7561 cggtaatatc atttcataac gagcaagtct cgatcctttg tcagaaacaa gtcgatagtc
     7621 actcccatga agttcgacac cccgcttctt ggccacagcg tcgatttctt taacccaata
     7681 aggcgatgaa tcaacattgg gaaaggaatc ataaaaaact tggatggcct gattatcatc
     7741 aagctgaatc ggttgtgaag cgtctcgttg agattgttgt tgcagcaagg tcactttcgc
     7801 ttgagcttga tcgagcgttt catattgtgg ccatactgca gctagaaaaa aaacgacgga
     7861 aagcaccatc aagccaaatc caagcttacc gactgcgctt aaacgcaagg tttgccagcg
     7921 aatctcactc aataaattgt ttaaggcgta tcgatccatg aggtaatcaa agaaaatagg
     7981 ataggtcgat gcggattttc ctgctttact ttataactga gtatatgtgc attttcgaaa
     8041 aatgcttcac gttccatggc ttcgacaaac tcagttaaag cagctagatt tctggcttcc
     8101 ccattaatcc gaatcgattg attggctacg ctgggttgca ccgataacag cgcaatatct
     8161 tttgttaccg tatattcaag tgaatcaaac aatacttccc agggtaaatt gagctgcttc
     8221 attatttctc gggcttgttt gagttcttgc tgtgattctt ggttggtccg tgagcttgat
     8281 gtttttgggg cacgcttttg ctgttgcgca ttctcaaagc gatctgctcg ggtttcccag
     8341 tacgaaattt cttccatgat cgattttaaa tgcaaaacgg tagcaactaa aaacactagc
     8401 cccagcagca gcaagccata atcaagtggg ctcgtttgct gtccggtata aggaaattta
     8461 aggctaagtc gacgcatgcg ctggaagctc gctggtatta cccactgggg ccggaaaatg
     8521 attaggagca agaatgccgt caaacggtat ccgattaaac tgccatccct tatctaaggg
     8581 taattgtaaa ttagtatgtt ctggcgcata gataaaagct aactcaacgg gttctcgttg
     8641 agtggcaatg attgcttcct gttcgagtaa tacgagtaac tcggtttcaa ctgaattcag
     8701 taatcgttga ttgcgaatac ttttccaacc tccattcgag aatagcagtg cacataggcg
     8761 acctggttca acgacaataa accaaaatcg ctgataattg attgcacgac tccattgatc
     8821 aaatgcggct gttaagtaag gctcaatctg tttgagcttc atatggaatc gtaccgttag
     8881 cgcttgcaat tgctgaacga ggtcgcgcgc taatgcggca caaataccgc ctaatttagg
     8941 atcccaatca ctcattgaaa tatcccaact atcgatgcga tcgccataga tttctcgcat
     9001 ctggaattgc gcataagcaa agaattcgtg ggcatctttg ataccttgct gaaactgaac
     9061 aacggcatag cgaacgaaat ggttggacaa ggtgatcgag agttcagcgc ccttcaaact
     9121 gctgacgcta ttttcttttt cgaggagctg cgtcaactgt ccgatcgcaa ttttccacaa
     9181 cgagttatcc gggtttgctt caaaattaat tgaaattttg ggctgcggta aagctttgag
     9241 tcccttggga attctgacta aatcaatcct gcctgacgca aagaaaatgt gaatttgatc
     9301 acgccacgat ggtgacac
//
LOCUS       DAOQKQ010000106         9231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_3580_length_9231_cov_6.410963, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000106 DAOQKQ010000000
VERSION     DAOQKQ010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 9231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9231
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3580_length_9231_cov_6.410963"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PKZ49_08050"
     CDS             <1..349
                     /locus_tag="PKZ49_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36552.1"
                     /translation="EMAKLEDGDEALAIHIGYESQPDDESLSEVFDHYVDEPRVSGAL
                     WRSYDRGKKIILLGGLLVGMAAVWFVTIFIPESSELAILATGLVAACLFILPTFLIGL
                     LIYRSKIKQSKHS"
     gene            382..1239
                     /gene="folD"
                     /locus_tag="PKZ49_08055"
     CDS             382..1239
                     /gene="folD"
                     /locus_tag="PKZ49_08055"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HND36553.1"
                     /translation="MSALIIDGKAIANKVRDALKQRTEKLAQLGLQPGLAVILVGDNP
                     ASGIYVRNKAKACKEAGIHSEIYQFPGDTSQMTVLEHIHTLNHDPKIHGILIQLPLPA
                     HFDTQKIIESIAIEKDVDGFHPCNIGALATGNARFYPCTPYGVMTLLKEFNLTIEGQH
                     AVVVGRSNIVGKPMAYMLLEKGATVTICTSKTQNLSQYTQSANILVVATGKPQMITAA
                     MVRPGSVIIDVGINRLGDGRLCGDVDFASVKEKVGYITPVPGGVGPMTITMLLSNTIE
                     AAERVQSAS"
     gene            1288..3951
                     /gene="aceE"
                     /locus_tag="PKZ49_08060"
     CDS             1288..3951
                     /gene="aceE"
                     /locus_tag="PKZ49_08060"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HND36554.1"
                     /translation="MNPHDIDPQETQEWLDALESVLINEGPERAHYLLERLVEKARRS
                     GAYLPYSANTAYINTIPSGKEERSLGDHAIEHRIRSYVRWNAMAMVLRANRNTNVGGH
                     IASFASAATLYDVGYNHFWHAPSSERGGDLIYVQGHSSPGIYAYAFLMGKLSTQQLDN
                     FRQETGGNGLSSYPHPWLMPDFWQFPTVSMGLGPLMAIYQARFMKYLGSRNLVETTGR
                     KVWAFMGDGEMDEPESLGAITLASRENLDNLIFVINCNLQRLDGPVRGNGKIIQELEA
                     AFRGAGWNVIKVIWGSYWDPLLAKDTKGLLQQRMMECVDGEYQTFKARDGAYVRKHFF
                     GKYPELLEMVANMSDDDIWRLNRGGHDPHKVYAAYAAAVKHQGQPTVILAKTIKGYGM
                     GESGEAQNITHQQKKMGTTSLRAFRDRFGLPVPDEKIDEIPYLQFDENSEELRYMKSH
                     REALGGFPHHRRRKAQPLNPPALTAFETVLKASGEGHESSTTMAFVRILNILTKDKNI
                     GKHVVPIVADESRTFGMEGMFRQLGIWSSVGQLYTPQDADQLMYYKEDKHGQILQEGI
                     NEAGALSSWIAAATAYSTHGVQMIPFFIFYSMFGFQRVGDLIWAAGDLRCRGFLIGGT
                     AGRTTLNGEGLQHEDGHSHLTASTIPNCVSYDPTFAYELAVIVQDGLRRMYQEQEDIF
                     YYITVMNENYAHPDLPKGAEQDILKGMYLLQDRSHQHKEKKIPLQLLGSGTILREVMA
                     AAEILQNDYGVAADVWSVTSFNQLRREALSVSRWNMLHPTKNAKTSHIENCLKNRNGP
                     VVAATDYMKLYADQIRAFIPGRYSVLGTDGFGRSDTREKLRHFFEVDRYYITVAALKV
                     LSEEGKIPAKLVSQAIKTLGIDPEKPEPITQ"
     gene            4020..5357
                     /gene="aceF"
                     /locus_tag="PKZ49_08065"
     CDS             4020..5357
                     /gene="aceF"
                     /locus_tag="PKZ49_08065"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HND36555.1"
                     /translation="MGQVKKVLVPDIGDFKEVPVIEVLIHEGERLSIDDALITLESDK
                     ASIEVPSPYAGIVNTIHVKVGDKVSEGAEIITMEVSDAEEKSSTTIQAEAVSDSPAAS
                     NVKQSIAKEIPVAAVEQSEAKSPAQQPSQLSNRSWNANPDITPHAGPSVRRFARELGV
                     DLTRVIGSGPKHRILKEDILAYVKHQLSKPTSSGERGYLDLLPWPHVDFAKFGPIELK
                     TLSRIKQISGANLHRNWVMIPHVTQFDEADITELESLRKEYNESQSANGIKLTILAFL
                     IKATTAALKKFPEFNASLDNSNSEANLVLKRYYHLGFAVDTPNGLVVPVIRDADQKGV
                     ITIAEELGKLSTLARDGKLKPTDMQGASFTISSLGGIGGTAFTPIINAPEVAILGVSK
                     ASLKPVYRNGEFVPRLVVPLSLSYDHRVIDGASAARFTSHLSNILADMRLALF"
     gene            5414..6088
                     /gene="nadD"
                     /locus_tag="PKZ49_08070"
     CDS             5414..6088
                     /gene="nadD"
                     /locus_tag="PKZ49_08070"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HND36556.1"
                     /translation="MKTDYTLIGIYGGTFDPIHYGHLRTAEELVEILQLDGLHFIPAG
                     QPRLRNQPIASRHQRCVMLNKAIRDNSRFELDTREIKGRGESYSVETLRKLNQEYSSR
                     QVAFCFIIGADAFMKLPSWYHWRELFDLCHLIVVNRPGFSLINQQSDLPHELTIECQD
                     RWVEQADLLKQRASGLIYCAATTPVAISATTIRNAILTGKSIRYLLPDSVINYIYRYH
                     VYPGEK"
     gene            6085..6435
                     /gene="rsfS"
                     /locus_tag="PKZ49_08075"
     CDS             6085..6435
                     /gene="rsfS"
                     /locus_tag="PKZ49_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HND36557.1"
                     /translation="MNTNQLIKITIEALEDIKANEIVVLEAEKNVSLFDYIVVASATS
                     SRQANAIANHIREKVKAAGGAIYGIEGERTGEWLLVDLGDVIVHIMQPTTRAYYNLEA
                     LWSESARYQTHPQP"
     gene            6437..6898
                     /gene="rlmH"
                     /locus_tag="PKZ49_08080"
     CDS             6437..6898
                     /gene="rlmH"
                     /locus_tag="PKZ49_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HND36558.1"
                     /translation="MKFNVLAVGNKMPDWIESGFMEYRKRMPKELSIELIEVKQVKDK
                     NREQSLLAEYERIAKVLPTANYIVAMDEQGKQLSTQELAASLINWMNLGKDITFIIGG
                     ADGLHPVIKEKADQVLSLSKMTLPHGLVRVLLAEQLYRATTILKHHPYHRA"
     gene            6969..7592
                     /locus_tag="PKZ49_08085"
     CDS             6969..7592
                     /locus_tag="PKZ49_08085"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HND36559.1"
                     /translation="MAYTENQIYLASRSPRRRELLKQIGIKHSILLMRETMDRSVDID
                     ETPDAEESPADYVYRMVHTKADASWKRLTQRKLPILPILVADTIVALDGCILGKPRDA
                     AHAEEMLLSLSGRTHQVLTAIALSMQGDVKHRLSITTVRFREIGKREIQACIKGGETH
                     DKAGAYAIQGLAAAFIVEIYGSYSGVVGLPLFETAQLLEESGVALFS"
     gene            7624..9078
                     /gene="rng"
                     /locus_tag="PKZ49_08090"
     CDS             7624..9078
                     /gene="rng"
                     /locus_tag="PKZ49_08090"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HND36560.1"
                     /translation="MNSEILINMTPQETRVAVIEQGVVQELHIERTSSRGIVGNIYNG
                     RVSRVLPGMQSAFIDIGLERAAFLHVADIKEFGSNTDADKPIERLLFEGTSILVQVIK
                     DAIGTKGARLSTQISIAGRMLVYLPYESHIGISQRIEDNAERESLREKLQRVLPPEDK
                     GGYIIRTMAEAASESDLRADIEYLHKLWHDIQNKSLSASPPALLYQDLDLSHRVLRDF
                     LDESITGIIVDSRETHQKLKAFAQNFMSNVTDKIDHYTGERPLFDLYGVEEEIEKSLS
                     RRVNLKSGGYVIIDQTEALTTMDVNTGGFIGVRNFDDTIFKTNLEAAQVIARQLRLRN
                     LGGIIIIDFIDMENQEHRNAVLVEFNKALMKDRTRMTVNGFTALGLVEMTRKRTRESL
                     AHILCETCPTCQGRGEIRTAQTICYEILRELLRESRQFNANEYRILASQPVIDLFLDE
                     ESQSLAQLGDFIAKPISLQVEASYTQEQYDVILI"
BASE COUNT         2624 a         1852 c         2223 g         2532 t
ORIGIN      
        1 ggagatggca aaacttgaag atggcgatga ggctttagcc attcatattg gatatgagtc
       61 tcaaccggac gacgagagct tatccgaagt ctttgatcat tatgtggatg aaccccgggt
      121 ttcaggtgca ttatggcgat cctatgatcg aggtaagaaa attattttat tgggtggctt
      181 gctagtcggg atggcagcgg tatggtttgt cacaattttc ataccagaaa gtagcgagct
      241 tgcgattttg gcaacggggc tagtggccgc ttgtttattt atcttgccta ctttcttaat
      301 tggtttgctc atctatcgca gcaaaattaa acaaagtaaa cattcttaaa gacaataagc
      361 ggggggttgt cttaaaaaat tatgagtgca ttaatcatcg atggtaaagc cattgccaat
      421 aaagttcggg atgcactcaa gcaacgtacc gagaaacttg cccaattagg gttgcagcct
      481 gggcttgcgg ttatcttagt tggagataat ccggcatccg gtatttatgt gcgtaataaa
      541 gcgaaagctt gtaaagaagc agggattcat tctgagattt atcaatttcc gggtgataca
      601 tcacaaatga ccgtgcttga gcatattcat accttaaatc atgatcccaa gatacatggt
      661 attttgatac aacttccatt acccgctcac ttcgatacgc aaaaaattat cgagtcgatt
      721 gcgatcgaga aagatgtaga tgggtttcat ccttgcaata taggcgcatt agcgacaggt
      781 aatgcgcggt tctatccgtg cacaccttat ggtgtaatga ccttattgaa ggagtttaat
      841 ttaactatcg aaggacagca tgcggttgta gtgggtcgaa gtaatatcgt tggcaaaccg
      901 atggcttata tgctgctaga gaagggtgcg accgttacga tttgcacatc caaaacacaa
      961 aatttatccc aatataccca gagtgccaat atattagtgg ttgcaacggg aaaaccccaa
     1021 atgattactg ctgcaatggt tcgtccggga tcggttatca tcgatgtagg aatcaatcgg
     1081 ctaggcgatg gtcgtctttg tggtgatgtt gattttgctt ccgtgaaaga aaaagtaggc
     1141 tatattaccc ctgtcccagg gggtgtcggt cccatgacca ttaccatgct attaagtaac
     1201 acgatcgaag ctgccgagcg cgttcaatct gctagctaaa atcaaggata ttgcttatcc
     1261 tttaattgaa cattacagtt actcgatatg aatccacacg atattgatcc gcaagaaacg
     1321 caagagtggc ttgatgcact ggaatcggtg ctcatcaatg aaggaccgga acgagcgcat
     1381 tatttattgg aacgcttagt tgagaaagca cgacgttcgg gtgcttacct tccttacagc
     1441 gccaatactg cttacattaa tacgatccca tctggcaaag aagaacgttc attgggcgat
     1501 cacgcaattg agcatcgtat tcgctcatat gtgcgttgga atgcgatggc gatggtattg
     1561 cgcgcaaatc gaaataccaa tgttgggggg cacatcgcaa gtttcgcttc ggcagctacg
     1621 ctctatgatg ttggttataa tcatttttgg catgcgccga gtagtgaacg tgggggtgat
     1681 ttaatctatg tccaaggcca ttcatcccct ggaatttatg cgtatgcttt tctaatgggg
     1741 aagttatcga cgcagcagct cgataatttt cgtcaagaaa ccggaggaaa tgggctttcg
     1801 tcatatcctc atccttggtt gatgccggat ttttggcaat ttcctacggt atcaatgggg
     1861 ctgggcccac ttatggctat ttatcaggcc cgttttatga aatatctagg aagtcggaat
     1921 ttagttgaga cgacggggcg caaagtttgg gcttttatgg gtgacgggga aatggatgag
     1981 ccagagtcgc tcggcgctat taccctcgca tcgcgtgaaa atctcgacaa tttgattttt
     2041 gtcattaatt gtaatctcca gcggctagat ggccctgtta ggggaaatgg aaaaatcatc
     2101 caggagcttg aagccgcatt taggggcgct ggctggaatg tcatcaaggt gatttggggc
     2161 tcctactggg atcctttgct agctaaggat actaaaggtt tgctacaaca acgtatgatg
     2221 gaatgcgttg atggtgagta tcaaacattt aaagccagag atggtgctta tgttagaaag
     2281 catttttttg gaaaatatcc tgagctactg gaaatggtag ctaatatgtc tgatgatgat
     2341 atttggcggc taaatcgtgg ggggcatgat cctcacaagg tttatgctgc ttatgccgca
     2401 gcagtcaaac atcaaggtca gccgaccgtt attctggcaa aaaccatcaa aggttatggg
     2461 atgggtgagt caggtgaagc gcaaaatatt acgcatcagc agaagaagat gggtacaact
     2521 tctctgagag catttcgtga tcgatttggc ttgccggttc cagatgaaaa aattgatgag
     2581 attccgtatc tgcaatttga tgaaaattca gaagaactgc gctatatgaa atcccaccgc
     2641 gaagcattgg gtggatttcc ccatcatcgc cgccgtaaag cgcagccatt aaatcctcct
     2701 gcattgactg cctttgaaac agtattgaaa gccagtggag aaggtcatga atcttcgacc
     2761 acgatggctt ttgtgcgtat tctcaatatt ttgactaaag acaaaaatat aggaaagcac
     2821 gtagtaccga ttgttgctga tgagtctcgt acctttggta tggaaggcat gttcagacaa
     2881 ttgggaattt ggtcttcggt cggtcagctt tatacaccgc aagatgcaga ccagctcatg
     2941 tattataagg aagacaagca tgggcaaatt ttgcaagaag gcattaacga ggcaggtgca
     3001 ttgtcatcgt ggattgctgc ggcaactgct tatagtaccc atggcgtgca aatgattcct
     3061 ttctttattt tttactcgat gtttggtttt caacgggtgg gtgatttgat ttgggctgct
     3121 ggtgacttac gctgccgtgg ttttctgatt ggtggtacgg ctgggcgtac gacactcaat
     3181 ggtgaagggt tgcagcatga agatggtcac agtcacttga cggcctcgac tatcccgaat
     3241 tgcgtttcat acgatcccac ctttgcttat gagctagcgg ttattgttca agatggattg
     3301 cgccgcatgt accaggagca agaagacatt ttctactata tcacggtcat gaatgaaaat
     3361 tatgctcacc ctgatttacc taaaggggct gagcaagata ttctgaaagg catgtatttg
     3421 ctgcaagatc gaagtcacca gcataaagaa aagaaaatac cgctacaact cctcggttca
     3481 ggaacgatac tacgcgaagt gatggccgct gctgaaattc ttcaaaatga ctacggtgtg
     3541 gctgcagatg tatggagtgt aacgagtttt aatcaattgc gtcgtgaagc attgagcgtt
     3601 tctcgttgga acatgcttca tccgaccaaa aatgcaaaaa catcgcatat cgaaaattgt
     3661 ttaaaaaata gaaatggacc tgtcgttgct gcgacggact atatgaagct atatgcagat
     3721 cagattcgtg cttttattcc agggcgttat agcgtgttgg gtaccgatgg atttggtcga
     3781 tccgatacgc gtgagaagct cagacatttt tttgaggttg atcggtacta tattaccgtt
     3841 gcggcactta aagtgttgtc agaggaagga aaaataccgg ctaaattggt ctctcaagcg
     3901 atcaaaacat tgggtattga ccccgagaaa cctgaaccca taacacaata aatgatgggt
     3961 gacgctatga gtagtcttta tctcagaaag acagtgatgt attaaattaa ggaacgtacg
     4021 tggggcaagt aaaaaaagta ttagtcccag atattgggga ttttaaagag gttccagtca
     4081 ttgaagtgtt gattcacgag ggtgaacggt tatccattga tgatgctttg atcaccctag
     4141 agtccgataa agcctcaatt gaggtgcctt ccccttatgc cggaatcgtt aatacaatcc
     4201 atgtaaaagt tggggataag gtttctgaag gcgctgaaat catcaccatg gaagtatccg
     4261 atgcggagga aaagagtagc actaccatcc aggctgaagc agtgagtgat tcgcctgctg
     4321 cgagtaatgt aaagcagagc atcgctaagg aaataccggt tgcagcagtt gagcaatccg
     4381 aagctaaatc acctgcgcaa caaccatcgc agctctctaa ccgatcatgg aatgccaatc
     4441 cagatattac accccatgca ggcccttcag tacgccgttt tgcgagagaa ctaggggtcg
     4501 atctaactcg agtgattggg agtgggccta agcaccgaat tcttaaagaa gatatcttgg
     4561 cttatgtcaa gcatcaatta tcgaagccaa caagctcagg tgaacggggt tacttagatc
     4621 ttttaccctg gccccacgtt gattttgcaa aatttggtcc catagaatta aaaacattaa
     4681 gtcgcatcaa gcaaatatcg ggagccaact tacaccgtaa ctgggtcatg attccccatg
     4741 ttactcagtt tgacgaagcc gatattactg agttggagtc actccgcaaa gaatataatg
     4801 aatcgcaatc ggccaatggg attaagctaa caatactggc tttcttgata aaagcaacta
     4861 cggctgcttt aaagaaattc cctgaattta atgcttccct cgataatagt aatagtgagg
     4921 caaatttagt actaaagcgc tactaccatt taggatttgc ggttgatacg cccaatgggc
     4981 tggttgttcc ggttattcgt gatgccgatc agaaaggggt tatcaccatt gctgaagagc
     5041 ttgggaagct ttcgacactg gctagagatg gaaaattaaa accaacggat atgcaaggtg
     5101 cgagtttcac gatatcaagc cttggcggca ttggtggcac ggcatttacc ccgattatca
     5161 atgctccgga agttgctatt ctaggtgtat ctaaagctag tttgaagcca gtttatcgca
     5221 atggagaatt tgtaccacgg ttagtcgtcc ccctttcgtt gtcctacgat caccgagtga
     5281 ttgatggagc atcagcggca cgttttacct cacatttgtc gaatattctt gccgacatgc
     5341 gccttgcgct tttttgagtg attgatagat cgatccacca tcaaacgctg gctttcctgt
     5401 cagcagtata ttagtgaaaa ccgactacac tttgataggt atttatggtg gcaccttcga
     5461 tccaatccat tatggtcatt taagaactgc agaagaactg gtagaaattc tacagctcga
     5521 tgggttacat tttataccgg ctggacaacc tcggctccgc aatcaaccca ttgcttcccg
     5581 gcatcaacgt tgtgttatgc tcaataaagc aattcgagat aatagtagat ttgagctaga
     5641 cactcgggaa attaaggggc gaggtgagag ttacagtgta gaaacgttaa gaaaattaaa
     5701 tcaggagtat tccagtaggc aggttgcttt ctgctttatc ataggcgctg atgcttttat
     5761 gaagttacca agctggtacc attggcgtga attattcgat ctttgtcatt tgatcgtggt
     5821 caatcggccc ggttttagtt tgattaatca acagtctgac cttccgcatg aactcacgat
     5881 agaatgtcag gatcgttggg ttgaacaagc ggatttatta aagcaacgtg catcaggtct
     5941 tatctattgc gctgcaacga ccccagttgc gatttctgca acaactattc gaaatgctat
     6001 cctaactgga aagagcatac gctatttact accagatagc gtaataaact atatttatcg
     6061 ctatcatgtg tatcctggag agaaatgaat accaatcaat taatcaaaat taccattgaa
     6121 gcacttgagg atatcaaagc aaacgagatt gtcgtgcttg aagctgagaa aaatgtttca
     6181 ctgtttgact atatcgttgt tgcgagtgcg acatcatccc gacaagccaa tgcgattgca
     6241 aaccatattc gagaaaaagt taaagcagcc ggtggggcaa tctatggtat cgaaggggaa
     6301 cgcaccgggg aatggctctt agtcgattta ggcgatgtga ttgtccatat tatgcagcct
     6361 acaacgcgcg cttattataa tttagaggcg ctatggtccg agtcagcccg ctatcaaacc
     6421 catcctcagc cttaacatga agttcaatgt gctggccgtg gggaataaaa tgccagactg
     6481 gatagaatca gggtttatgg aatatcgtaa acgcatgccg aaggaactat caatcgaatt
     6541 gattgaagtt aagcaggtca aagataagaa tcgtgagcaa tcgcttcttg cagaatatga
     6601 gcggatagcg aaagtattgc cgactgcaaa ctatattgtt gctatggatg agcaaggcaa
     6661 acagctatcg actcaagagc ttgccgcttc attaataaat tggatgaact tgggcaagga
     6721 tattaccttt atcattggcg gtgctgatgg attgcatcca gtgatcaagg aaaaagcaga
     6781 tcaagtttta tcgctttcga aaatgaccct tccacatggt ttagtcagag tactattggc
     6841 agaacaatta tatcgtgcaa cgaccatcct caaacatcat ccctatcatc gtgcatagat
     6901 aacgtgtttc agggataatc acagctagag agcaattatc ttttgcctac cgttctgagt
     6961 taaaaattat ggcctatact gaaaatcaga tatacctggc atctcgcagc cctcgaaggc
     7021 gggagttatt aaaacaaatt ggtattaagc atagtatatt attgatgcgt gaaacgatgg
     7081 atcggtctgt tgatattgat gaaacacccg atgcagaaga atctccagca gactatgttt
     7141 accggatggt gcataccaag gccgatgcga gctggaaaag attaacacaa cgtaagttac
     7201 cgattttgcc aatcttagtc gccgatacca ttgtagcgct tgatggctgc atactcggta
     7261 aacccagaga tgcagcgcat gcggaagaaa tgcttttatc actctcaggt agaacgcacc
     7321 aagtattaac tgccatagca ttaagtatgc aaggcgatgt taaacatcga ttatcgatta
     7381 caacggtgcg ctttcgcgaa attggcaaac gggagattca agcctgtatc aaaggggggg
     7441 aaacccacga caaagcaggt gcctatgcga ttcaagggtt ggctgcggcg tttatcgtag
     7501 agatctatgg tagttatagt ggggtggttg gtttgccctt atttgaaaca gcccaactgc
     7561 ttgaagagtc aggggtagcg cttttttctt agtcgatact ttttctgttt cgatcgcttt
     7621 agtatgaata gtgaaattct catcaatatg acccctcaag agacacgtgt tgctgtcatt
     7681 gagcaagggg tggtccaaga attacatatc gagaggacca gtagtcgtgg catcgtagga
     7741 aatatctata atggtcgtgt gagtcgggtg ttaccgggaa tgcaatcggc tttcattgat
     7801 attggcctag aacgagcagc ttttctacat gttgcagata tcaaagaatt tggatcaaat
     7861 accgacgctg acaagcccat agaacgctta ctgttcgaag gcaccagtat cttagttcaa
     7921 gtcatcaagg atgcgattgg caccaaaggc gcccgcctct caactcaaat aagcatcgct
     7981 ggtcgcatgc tcgtttattt accctatgag tcccatatcg gtatctctca gcgtattgag
     8041 gataatgcag agcgtgaatc cttgcgagaa aagctgcaac gggttttacc cccagaagat
     8101 aagggagggt atattatccg aacgatggca gaagcggcca gtgaatccga tttgcgcgca
     8161 gatatcgaat atttacataa gctttggcac gatattcaga ataaatcctt atcagcgagt
     8221 cctccggcat tgctctatca agatctcgat ctaagtcacc gagtactcag agattttttg
     8281 gatgaatcga taacgggcat catcgttgat tcgagggaaa cccatcaaaa attaaaagca
     8341 tttgctcaaa attttatgag caatgtcacc gataaaatcg atcattacac cggcgaacgc
     8401 cccttgttcg atttatatgg tgttgaagaa gaaattgaaa aatcgttgtc acgccgtgtt
     8461 aatttgaaat cggggggcta tgtcatcatc gatcaaactg aagctttaac aaccatggat
     8521 gttaatactg gggggtttat tggggtccga aattttgatg atacgatttt taaaacgaat
     8581 ctcgaagccg ctcaagtgat tgcgcgacaa ctgcgccttc gaaatttagg cggaattatc
     8641 attattgatt tcatcgatat ggaaaaccag gaacatcgga atgccgtact cgttgaattc
     8701 aataaagcat taatgaaaga ccgcacacgc atgacggtta acgggtttac tgcactcgga
     8761 ctggttgaaa tgacgcgtaa acgaacgcgt gaaagtctag cgcacatcct atgtgaaacc
     8821 tgtcctacct gccaaggtcg aggtgagatc cgaacggcac aaaccatctg ttatgaaatt
     8881 ttgcgggaat tgctcagaga atcgcggcag tttaacgcca atgaatacag aatccttgct
     8941 tcacaacccg tgatcgattt atttctggac gaagaatcgc aaagcctagc gcagttaggc
     9001 gattttatcg caaaaccgat cagcctccag gtagaagcat cctatacaca agagcaatat
     9061 gatgtcatct tgatttagga atgaatgtaa gagcggctag aaaggttgtc tgtgatttta
     9121 taatcacacc actcatgaag gggtaggtta ttacagcgca gcttcttgcg cgtcgcctgc
     9181 aaccgggggg gtaagttcgc attttacggc gcgtttgcga gcgtggcaac g
//
LOCUS       DAOQKQ010000107         9076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_3668_length_9076_cov_6.129365, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000107 DAOQKQ010000000
VERSION     DAOQKQ010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 9076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9076
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3668_length_9076_cov_6.129365"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="PKZ49_08095"
     CDS             <1..662
                     /locus_tag="PKZ49_08095"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HND36561.1"
                     /translation="VSGLETRLRYFVQVWRKENNKLQKGVWLDLGCGAGTYSKFLVDQ
                     GMYVIGLDYSFPTLKKAQLRNYSSIEWCVADATKIPLRTGIFDGAICFGVTQALENSD
                     TVIAELASATKVGGKIWVDALNRWCIVHFIESFLRRLQGKPMHLRYETPANIINLMRK
                     NNLSNIQLFWLPMLPSKLARFQWVLETPFLKWVFRNVPLMGSSLCHAFIITAERNYPS
                     D"
     gene            707..1663
                     /locus_tag="PKZ49_08100"
     CDS             707..1663
                     /locus_tag="PKZ49_08100"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HND36562.1"
                     /translation="MKLLPLIFSPSGKRARLSIVIYHRVLLQPDLLVGQKGDIASFDT
                     HLRILTRNFNILPLSKAVNMLKSNTLPSRAVSITFDDGYADNAEIALPILKKYSTVAT
                     FFVSAGVIDGGRMWNDTIIESIRHAQGDILDLSQLNMGSYPIKSLTERQKVLITLIEK
                     LKYTPQEQRQEFVDKLSALIAAKLPNNLMMTSDQVRELHREGMEIGAHTINHPILARI
                     ENPTAYNEIAEGKKMLEEIIKAPVNLFAYPNGKPNKDYLLDHVKMVKEIGFDAAVSTA
                     WGAAQAGDDIYQLPRFTPWDLNEGFFVLRMMRNMFNEIEVAH"
     gene            complement(1688..2197)
                     /locus_tag="PKZ49_08105"
     CDS             complement(1688..2197)
                     /locus_tag="PKZ49_08105"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HND36563.1"
                     /translation="MAREKVELTIPTLVILLSLITGTLIYFIPRPIITQIYENYAENY
                     QVIEVNPRVFIYLDTDSSITIRRNEPIQIELIRGGASFDIEEAKSKILDQLEIIIGDA
                     RITNKGTRFNIKIYDKRSEITITDGQIELHLPSQKYLIDSGRKIEIDNNQIITDNIIN
                     RTEVPPWLK"
     gene            complement(2286..2705)
                     /locus_tag="PKZ49_08110"
     CDS             complement(2286..2705)
                     /locus_tag="PKZ49_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HND36564.1"
                     /translation="MSFLNQERYQSQSTMSEINVVPLVDVMLVLLIIFIITAPLLTHS
                     VKVDLPKAASNPNITQPDHIEFAIRADGSFFWNGEAIHFYQLSQRFTEAAKLSQTTEL
                     HIRADKHAHYEYVARVMSIAAKSGLSKIGFITDPTIE"
     gene            complement(2705..3466)
                     /locus_tag="PKZ49_08115"
     CDS             complement(2705..3466)
                     /locus_tag="PKZ49_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HND36565.1"
                     /translation="MENGLGFSNFLAQIDQIGTSVLILLLSLSVASWYLIISKSITNL
                     IATKRANLFLENFWKLNSLQELQLNIKNLPNDNAFSELAKQAIETTKNSQRVGLEKIA
                     TSGTNELLTRTLRNGLEQEATRIEGGLTIIASAGSAAPYIGLFGTVWGIYHALIQIGL
                     SGQGTLDKVAGPVGEALIMTALGLAVAIPAVLAYNAFTRRNRIWMSKLDAFAHDLYTI
                     ITVDNQSNKQPEAITQAQPRSFSAAVDTPVFEVKQ"
     gene            complement(3471..4181)
                     /locus_tag="PKZ49_08120"
     CDS             complement(3471..4181)
                     /locus_tag="PKZ49_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HND36566.1"
                     /translation="MTGAKNKPLITSLSFNSLLLVLMVHAGLLYAMWHHRFPQPSLQT
                     VTLFAELISPPQKVEPANPTKVDLKAEPKPTPPEKPKVTKKIQPKQQQVAAHAPAKPK
                     EATIAPPVVNEPIKETDSSPPQEVKPAVASRPQMSADPITLSSELSVSCPELSAPDYP
                     AISRRLGEEGKLMLQVELDESGRISNTRIINSSGYSRLDNAALAAVKTWRCKPAVRDG
                     QPVKAIALQPFNFVIQGN"
     gene            4432..4848
                     /locus_tag="PKZ49_08125"
     CDS             4432..4848
                     /locus_tag="PKZ49_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HND36567.1"
                     /translation="MNPDTLSFWRRTLCLIYDFLLFLAIWFIASLIFIVIVQDTSFAY
                     FRPIYQFYSLSIIGIYFIWFWTHGGQTLAMQTWKLRVVAQDGKALTLRQAITRYLFAV
                     VGITSFGLGIIWALFDRDRKFLHDRLAGTRIIRVEQ"
     gene            4891..6129
                     /locus_tag="PKZ49_08130"
     CDS             4891..6129
                     /locus_tag="PKZ49_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HND36568.1"
                     /translation="MLSRASLNSQIVVGVLLGVLIGAWLTTLDQTTQLAQQSLYFASL
                     ASTLFIDLLKMVLVPLVFSSIVVGVANLQTHHQINRVWVITLAYFLLTMMLAVLLGLF
                     VTNSLRPGEELQIAMFQDMMQASEVKQMSWQAFFAHFLHSLFLNPLAAMAQGQILAVV
                     VFALLFGVALVVGGDRYTNILNLMKELLELILMLVNWIMRLAPFGIMALLIQLIVSQD
                     IELLMTLIQFVAVVIGTTLFHGLIILPLILYLTTGTTPLKLWKGSREALITALATSSS
                     AATLPITLRCVEQYLHVKPDVARFVLPVGATMNMDGTALYEAIAALFIANLAGIDLNL
                     TQQLIIFFTAMFAAIGAPGIPSAGMITMVMVLQAVGLPVEAIAILLPIDRLLDTFRTM
                     VNVEGDIVGSMVVQHWVDKT"
     gene            complement(6140..7990)
                     /locus_tag="PKZ49_08135"
     CDS             complement(6140..7990)
                     /locus_tag="PKZ49_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HND36569.1"
                     /translation="MLNFFERLINPFPPEHPTQPPNGLFPFCKHYIRGIERYLVLMAI
                     LTIAVAVGEAMLYGVLGTIIDWLSEKDPSRFLQEEYYSLIGLSIFILIVMPMLVIAHS
                     LVMYQTLMGNFPMIVRWQAHRYLISQSYAFFQNEFSGRIATKVMQTALAVRETVMKTI
                     DVLLFVSVYFATTLFLIANADFRLCLPLFFWLSIYLCILYYFIPKLRDISKLQADARS
                     VMTGRIVDSYTNILTLKLFSHTHRESEYVKSSMVNFLGTVHPQMRLVTWLHSFVWSNN
                     MLLVFCIGGLGIYLWINGNITIGAIAVALSLAIRLTGMSHWVMWEISALFENIGIVQD
                     GIDTLSKPHTVTDINDAPLLTVSSGSIHFKQISFSYQSDTQNKIIHQPIFRNFDLTIA
                     AGEKVGIVGPSGAGKSTLVNLLLRFYDVQQGNIYIDEQDISLVAQESLRTHIAIVTQD
                     TSLLHRSIRDNILFGKPDATEAQMIEAAKLAHAHEFITTLVDSKGRRGYDAFVGERGV
                     NLSGGQRQRIAIARVLLKNAPILILDEATSALDSEIEASIQENLYQLMQGKTVIAIAH
                     RLSTIAAMDRLIVMDKGQIIEQGSHAELLSHNQLYATLWNHQSGGFLGHL"
     gene            complement(8061..>9076)
                     /locus_tag="PKZ49_08140"
     CDS             complement(8061..>9076)
                     /locus_tag="PKZ49_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HND36570.1"
                     /translation="ALAKMDLLQVIKKGQASMVAASLALGAFQQRMAEQLGVEPGSEM
                     RTAIKDATDAKLPVLLIDREIGTTLKRIYYNVPWWRRFELYSGLLASIITRESVSSEE
                     IEKLKEGDVLESAFLQFSQDQKDLFKPLISERDEYMSARLINECKENNYKHTLAIVGA
                     GHLKGIKELLMNRSISNTNQKLEELDTIPNTSSWFKYLPWIIVILILTGFAFGFDRSS
                     DIGMAMIIDWVFINGGLAALGAIIALAHPLTILTAFLAAPITSLNPMIGAGMIAATAE
                     IFIRKPQVEDFGRLRSDAATLKGWWHNQVTRILLIFLLSSFGSALGTYIAGFRIFERL
                     SSG"
BASE COUNT         2705 a         1731 c         1847 g         2793 t
ORIGIN      
        1 ctgtctctgg cttagaaaca cgcttacgct attttgtaca agtttggaga aaggaaaata
       61 ataaacttca aaagggagta tggctagatc ttggttgtgg agcaggaact tactctaaat
      121 ttcttgtaga tcaagggatg tatgttatcg gtctagatta ttcattcccc actttaaaga
      181 aagctcaatt aagaaattat tcctcaatag aatggtgtgt tgcagatgca actaaaattc
      241 cattacgcac tggtattttt gatggtgcta tttgttttgg cgtaacacaa gcacttgaaa
      301 actctgacac tgtgattgct gaactagctt cagctacaaa ggtaggtgga aaaatctggg
      361 ttgatgcatt aaatcgatgg tgcattgttc attttattga aagttttctg cgtagactac
      421 aaggaaaacc catgcacctt cgttatgaaa ctcctgctaa tattatcaat ttaatgagaa
      481 aaaataatct aagtaatatt cagttatttt ggttaccaat gcttccttca aaattggctc
      541 gtttccagtg ggtactagaa actccttttt taaaatgggt gtttcggaat gttccattaa
      601 tgggaagttc gttatgtcac gcatttatta ttactgctga gagaaactac ccaagcgact
      661 gatagtaaaa ttactagaaa aaaattaaga ttaaaatgaa tctcccatga aactcctacc
      721 attgattttc tcaccgtcag gtaaaagagc tcggttatcg attgttattt atcatcgtgt
      781 tttacttcaa ccggatcttc ttgtgggtca gaaaggagat attgcaagtt ttgatactca
      841 tctgagaatt ttgactagaa attttaatat tcttccatta tccaaagcag taaatatgct
      901 taaaagtaat actttaccca gccgtgcagt cagtattaca ttcgatgatg gatatgcaga
      961 taatgcagaa attgcattac caattttaaa gaaatattct acagtagcta ctttttttgt
     1021 ttcggctggc gtgattgatg gtggaagaat gtggaatgat acgattatag aatcaattcg
     1081 ccatgctcaa ggagatatat tagaccttag ccagctcaat atgggaagtt atccaataaa
     1141 atccctgact gaacgtcaga aagtattaat cacattaatt gagaagttaa aatatacgcc
     1201 tcaagagcaa agacaagaat ttgtagataa gttgagcgca ctcatcgcgg caaaattacc
     1261 aaacaatcta atgatgacat cagatcaagt tagagaacta catcgtgagg gaatggaaat
     1321 tggtgcacat actataaacc atccgattct ggccaggatt gaaaatccaa ccgcttataa
     1381 tgaaatcgct gaagggaaaa aaatgcttga agaaataatc aaggcaccag tcaatttatt
     1441 tgcgtatcca aatggaaaac ctaataaaga ctatttgctt gatcatgtca aaatggtaaa
     1501 agaaataggt tttgacgcag cagtgtcaac agcctggggc gctgcccaag caggtgatga
     1561 tatctatcaa ttgccaagat ttacgccttg ggatctgaat gagggtttct ttgtattacg
     1621 catgatgcgg aatatgttca acgaaatcga ggtagctcac tgacagtact aaaccttaca
     1681 agtataatta ttttaaccaa ggaggcactt cagttcgatt gataatatta tcagtaataa
     1741 tctgattatt atcaatttca attttccttc ctgaatcaat gaggtatttc tgggatggca
     1801 agtgtaattc aatttgtcca tcggtaattg tgatctcgct acgcttatca tatattttga
     1861 tattaaaccg agtgccctta ttagtaatgc gagcatcccc aatgataatt tctaactgat
     1921 ctagtatttt tgattttgct tcttctatat caaaagaagc tccaccacgt attaattcta
     1981 tttgtattgg ctcgtttctt cgtattgtga tcgaactatc cgtatctaaa tatataaaaa
     2041 ctcgaggatt tacttcaatt acttgatagt tttcagcgta attttcataa atttgcgtaa
     2101 tgattgggcg aggaatgaaa taaattaatg tgccagtaat aagcgacaat aaaataacta
     2161 gcgtaggtat agtcagttct actttttctc tagccataag aaaaaagaat actttttctc
     2221 aaattccaaa aatagagtaa gtctcttatg gctatggcta aagagcgtat aacgctatac
     2281 aacaattact ctatggttgg atcagtaata aatccaattt tagataaacc ggacttggct
     2341 gcgatactca taacgcgagc gacatactca taatgcgcat gtttatcggc tcggatatgc
     2401 aattccgttg tttgggaaag ttttgctgct tcggtaaatc gttgactaag ctggtagaaa
     2461 tgaattgctt caccattcca gaaaaagcta ccgtcagctc gaatcgcaaa ctctatgtga
     2521 tcaggttgag taatattggg attactcgct gctttaggta gatcaacttt gactgaatgc
     2581 gtaagtaaag gcgcagtaat gataaaaatg atgagcaaca ctagcatcac gtcaaccaat
     2641 gggacaacat tgatttctga cattgtcgat tgactctgat accgttcttg attaagaaat
     2701 gacattactg ttttacctca aacacaggtg tatcaactgc tgctgaaaaa gaacggggtt
     2761 gagcttgtgt tattgcttca ggctgcttat tagattgatt atcaacagta ataatcgtat
     2821 agagatcgtg tgcaaaagca tccaatttcg acatccatat tcgatttcgc cgagtaaagg
     2881 cgttgtaggc taatactgca ggaattgcaa cagccagccc taaagcagtc ataattagcg
     2941 cttcaccgac aggtccggct actttatcta aagtaccttg cccagagagt ccaatttgaa
     3001 ttaatgcatg atagatgccc cataccgtac caaataatcc gatataaggt gcagctgaac
     3061 cagccgaagc aataatggtt aaccccccct cgatacgagt ggcttcttgt tccaatccat
     3121 ttcgtaatgt acgcgtgagt aattcgttgg ttccactcgt agctatcttc tccaatccta
     3181 ctctctgaga atttttggtg gtttcaatcg cttgctttgc taattctgag aaagcattat
     3241 cattcggtaa gttttttatg tttaattgca gctcttgcag ggaatttaat ttccaaaagt
     3301 tttctagaaa taaattagca cgcttcgtgg caattaagtt agtgatactt tttgaaataa
     3361 tcaaatacca acttgcgaca gaaagcgata gcagtaagat gagtacactt gttccgattt
     3421 gatcaatctg agcaagaaaa ttagaaaatc ctaatccatt ttccattaaa ttaatttcct
     3481 tgaataacaa aattaaaagg ttgtaaagcg atagccttga cgggttgacc gtccctaaca
     3541 gcgggtttac agcgccaagt ttttactgca gcgagcgcag cattatcgag gcgagagtaa
     3601 ccactactat taatgatgcg ggtattacta attcgtcctg attcatccaa ttcaacttgc
     3661 agcatgagct taccttcttc tcctagccga cgggaaatag ccgggtaatc cggcgcgctg
     3721 agttcagggc acgaaactga taattctgag gatagcgtta tcggatcagc tgacatttgc
     3781 ggacgagatg ccactgcagg tttgacttct tgaggtggag atgaatcagt ttctttaatg
     3841 ggctcattga ctacgggtgg tgctatcgtt gcttctttag gctttgcagg cgcatgtgcc
     3901 gctacttgtt gctgtttagg ttgaattttt ttagtaactt taggtttttc aggtggagta
     3961 ggcttaggct cagctttaag atcaactttt gtagggtttg ctggctcaac tttttgaggg
     4021 ggtgaaatta attctgcaaa taacgtaaca gtttgtaatg aaggctgggg gaagcgatgg
     4081 tgccacattg catagagtaa tcctgcatga accatcaaca cgagtaacaa actgttaaac
     4141 gatagtgaag tgataagggg tttatttttt gcaccagtca ttcaatgagc tattttttta
     4201 gggcaatgat tgggtaaaat tattattttg attagtaaca aatactaacg caaacaataa
     4261 taattatcaa tcacttgtga tctccttgta gcttcttttt gtgatattga gtaatgacag
     4321 actttacaaa agaggctgat aaattagtgt agtatgaatt tgttactaag attcttttcg
     4381 gattaacatc ctagatgtaa attaccaatt ttgtttaatt taacttattc aatgaatcct
     4441 gatactttat cattttggcg acgcacgctt tgcctgattt acgatttttt attgttttta
     4501 gcgatttggt ttattgcaag cttgattttt attgtaattg tacaagatac cagctttgcc
     4561 tatttccgcc ccatatacca attctattca ctgtcaatta ttggaatcta ttttatttgg
     4621 ttttggacac atggaggaca aacacttgca atgcaaacct ggaaattacg tgttgtagca
     4681 caagatggta aagcacttac tttgcgacaa gcgattactc ggtacttatt tgcagtagtc
     4741 ggtataactt cgtttggact tggaatcatc tgggcacttt ttgatcgtga ccgaaagttt
     4801 ctacatgatc gactagcagg aactcgtatc atcagggtcg agcaataaac gaggctcaat
     4861 catcctgtat ttaatacaaa aatatcatcc atgctatcaa gagcttcact caatagtcag
     4921 attgtagttg gtgtgttatt aggtgtttta atcggggctt ggttaaccac attagatcaa
     4981 acaacacaat tagcgcagca aagcttgtac tttgctagct tagcaagcac tttatttatt
     5041 gatttgctca aaatggtgct tgttccactg gtgttttcgt cgattgtagt gggcgtggct
     5101 aatttacaaa cccatcacca aatcaatcgc gtatgggtta ttacgctagc ctattttcta
     5161 ttaaccatga tgcttgcagt tttacttggg ctatttgtta ccaatagctt gcggcctggt
     5221 gaagaactac aaattgctat gtttcaggat atgatgcaag cctccgaagt taagcaaatg
     5281 tcttggcaag cgtttttcgc tcattttctg cattcgctat ttctcaaccc attggcagcc
     5341 atggcgcaag gtcaaatcct ggccgttgtc gtttttgcat tactatttgg tgttgcgctg
     5401 gtggtggggg gagatcgtta tacaaacatt ctgaatttaa tgaaagagct tttagagctt
     5461 atcttaatgc ttgtgaattg gattatgcga cttgcgccat ttggaattat ggctttattg
     5521 attcaattaa tcgtttccca agatattgaa ttgttaatga cgctcattca atttgtggct
     5581 gtcgttattg gcacaacgct ttttcatggc ttgatcattt taccattgat cctttatttg
     5641 acgacaggaa ccacaccgct taaattgtgg aaaggatcgc gtgaggcact aattacagca
     5701 ctagcaacga gttctagtgc tgcaacatta cccataacat tacgctgtgt ggaacaatat
     5761 ctccatgtaa aacctgatgt agcgagattt gtcttgccag taggcgcgac catgaacatg
     5821 gatggtaccg cgctgtatga agcgatagca gcgttattta ttgcaaatct tgccggtatt
     5881 gatttaaatc ttactcagca gttgatcatt ttttttactg caatgttcgc cgcgattggc
     5941 gctcctggta tacccagtgc aggaatgatc actatggtaa tggtgcttca ggctgtaggg
     6001 ttaccagttg aggcaatcgc gattttattg ccaatcgacc gattattaga cacatttcgg
     6061 acgatggtta atgttgaagg ggatattgtt ggaagtatgg ttgtacaaca ttgggtggat
     6121 aagacttaat caatacttcc tataaatgac ccagaaatcc tccagattga tgattccaaa
     6181 gggttgcgta tagttggtta tgggatagta gctcggcatg actaccttgc tcgataatct
     6241 gacctttatc catcacaatc agtcgatcca ttgcggcaat ggtagacaat cgatgtgcaa
     6301 tcgcaatgac tgttttgcct tgcattaatt ggtataaatt ttcctgaatg ctcgcctcaa
     6361 tttctgaatc taaggcagag gtagcttcat ctaagataag aattggggca tttttaagta
     6421 atacgcgtgc gatagcaatg cgctgtcgtt ggccgcccga taaattaaca ccacgctctc
     6481 cgacaaacgc atcataacct ctgcggccct tgctatcgac cagcgtagtt atgaattcat
     6541 gggcatgggc taatttagcc gcctctatca tttgggcctc ggttgcatcc ggtttaccaa
     6601 ataaaatatt gtcacggatc gatcgatgga ggagggaagt atcttgcgtt acgatcgcga
     6661 tatgagtacg taaactttcc tgagcaacta agctgatatc ttgttcatcg atgtagatat
     6721 tgccttgctg aacatcataa aaacgaagta ataaattaac taatgttgat tttccagctc
     6781 cagatggacc aacaatgcca actttttctc ccgctgcaat cgttagatca aaattcctga
     6841 atatgggctg atgaatgatt ttattttgag tgtcagactg atacgaaaag ctgatttgct
     6901 taaaatgaat ggatccagag ctgactgtta ataagggagc atcgtttata tccgtcacag
     6961 tatgtggctt ggacagcgta tcaataccat cttgcacgat accaatattt tcaaataacg
     7021 cactgatttc ccacatgacc cagtgcgaca tacccgttaa tcgaatggcc aagctcagtg
     7081 caaccgcgat tgcaccaatc gtaatattcc cattgatcca taaataaatg cctaatccgc
     7141 caatacaaaa caccagcaac atgttgttac tccaaacgaa agaatggagc caagtcacta
     7201 ggcgcatttg tggatgaacg gtcccaagaa aattcaccat actagattta acatattctg
     7261 attcacgatg cgtatgggaa aataacttta acgtgagtat attggtatag ctgtcgacga
     7321 ttcgtcccgt catgacggat cgtgcatccg cttgaagctt agaaatatcc cttaatttag
     7381 gaataaaata ataaagaatg cagaggtaaa tgctcagcca gaaaaataag ggtaggcata
     7441 agcgaaagtc agcatttgca attaaaaaaa gcgttgtagc aaagtaaact gacacgaaaa
     7501 gcagcacatc aattgttttc atgacggtct cacgaacggc cagcgcagtt tgcatgactt
     7561 tggttgcaat acgaccactg aattcattct gaaaaaacgc atagctctgg ctgattaagt
     7621 aacgatgcgc ctgccagcga acaatcattg gaaaattacc catcagggtc tgatacataa
     7681 ccagtgagtg agcgatcact aacataggca ttactatcag gataaaaatc gacaacccaa
     7741 taagtgagta atattcttct tgcaaaaaac gactagggtc cttttctgat aaccaatcga
     7801 tgattgttcc taacacgcca tagagcattg cttcaccgac tgcaactgcg atggtcagga
     7861 tagccatcaa cacgagatag cgttcaatac ctcggatata gtgcttgcaa aatgggaaaa
     7921 gcccattggg cggttgagta gggtgctcag gcgggaatgg gttgattaga cgctcaaaaa
     7981 aattaagcat agtatttacc caagctggca aagaagtagg aagatacccg catctgaagg
     8041 attcgagtta tcttatgcta ttaaccactt gatagccttt caaatatcct gaagcctgca
     8101 atataggtac ctaatgctga gccaaaagac gaaagaagaa aaataagtaa tattcgagtc
     8161 acttggttat gccaccatcc ctttaaggta gcagcatcgc ttcttaagcg accaaaatct
     8221 tctacttggg gtttacgtat gaatatttct gcagtagcag caatcatgcc cgcaccgatc
     8281 attggattga gtgaggtaat cggtgcagct aaaaaagcag ttaggatagt caatgggtgt
     8341 gccagggcta taatggcacc gagggcagct aatccgccat taatgaagac ccaatcgatg
     8401 atcattgcca tgccgatatc tgagctacga tcaaaaccaa aagcaaagcc agttaaaatt
     8461 aaaataacaa ttatccaggg taggtatttg aaccaggaag aagtgttcgg aatcgtatca
     8521 agttcctcaa gtttttgatt cgtattggag atgctacgat tcattaagag ttctttgatg
     8581 ccttttagat gcccagcacc aactatggca agggtatgtt tataattatt ttccttgcac
     8641 tcattgatca agcgcgctga catatactcg tctctttcgc tgatcaacgg tttaaataaa
     8701 tccttttgat cttgggaaaa ttgcaagaaa gcgctttcta aaacatcacc ttctttcaat
     8761 ttctcaattt cttctgaact gacagattca cgcgtgataa tacttgcgag taatccgcta
     8821 tataactcga atcttctcca ccagggtacg ttatagtaaa tgcgctttaa tgttgtacca
     8881 atttctctgt cgatcaacaa taccggtaat ttagcatcag ttgcatcttt aatggcggtt
     8941 ctcatctccg atccaggttc aacacctagt tgttccgcca ttctttgctg gaaggcccct
     9001 aaagctaggc ttgcagcaac catgctggct tggccttttt tgatcacttg gagtaaatcc
     9061 attttcgcta atgcat
//
LOCUS       DAOQKQ010000108         8944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_3732_length_8944_cov_6.891439, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000108 DAOQKQ010000000
VERSION     DAOQKQ010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 8944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8944
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3732_length_8944_cov_6.891439"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="PKZ49_08145"
     CDS             complement(<1..1001)
                     /locus_tag="PKZ49_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HND36571.1"
                     /translation="MEEVFLGRQPILDRNQNLVAFELLFRAGEINEANVTNDVHASAS
                     VMVNAYGQLGIQRVLGQQRGFINISTELLMSDAILLLPSNHIVLELLESIEITDQVVK
                     RCLELKQIGYHLALDDVTEINSRTLQMLPIVNVVKLDVLAIDETKLKEIINTLRRWPV
                     ILLAEKVDSPELAKKCTALGFDMFQGYHFAKPEIISGKRVDANKLPLLNLLTMVMGDC
                     EAEEIETELKHQPNLSYNLLRLVNSVGFGLQQRINSIKQAVIILGRQQLQRWVQLLLY
                     TSDSSGDSISNALMQTAAVRGKLMELIAQIDRPHDKTYQDRAFMVGIFSLLDTLLGM"
     gene            complement(1089..1892)
                     /locus_tag="PKZ49_08150"
     CDS             complement(1089..1892)
                     /locus_tag="PKZ49_08150"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HND36572.1"
                     /translation="MKSLKKEKLLRLINTSGVGMRKPENEGGSNSLTAANEYYQQVEQ
                     FAQRIRKTNDVDEMIKILDTLLNETKGLHNNNEIQNTRELRHAENKIESLKYELEELR
                     ELVHTDQMTGTYNRRGLDNIFMREAARADRYENALCVVLIDLDDFKFINDTHGHLFGD
                     SALIHFATIAKKTIRPSDVIARYGGEEFLIILPDTRPEEAIWVMQRLQNNLSKNPLLD
                     TEETPIKITFSGGITLRKFQETQNTVIKRADKALYRAKQYGKNRIEIIA"
     gene            complement(2081..2713)
                     /locus_tag="PKZ49_08155"
     CDS             complement(2081..2713)
                     /locus_tag="PKZ49_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HND36573.1"
                     /translation="MIRVMIADDHAIVRQGLKQILSETGDISVTGEAETGFQAVKVIR
                     QQEFDVMLLDISLPDRNGIEILKQIKKDRPNLMVLMLSMHNENEFALRALKAGASGYL
                     NKQSAPAQLVTAIRQVSEGHKYISPALAQELANAWTSEADRPLHENLSDREFQTLCLI
                     ASGKSLSEISIEMCLSPKTVSVYRARMMEKLKISNNSELVRYAIKHKLVD"
     gene            complement(2746..4233)
                     /locus_tag="PKZ49_08160"
     CDS             complement(2746..4233)
                     /locus_tag="PKZ49_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HND36574.1"
                     /translation="MSESLSILLVGFLNQSAQTIRKIFTDEDINFDSKLVGTYDEFLH
                     EIQKCHWDIIIANEATMPTGTHAILTYFSQVNQYLRIPVIIISNESNEQSAKDFLKAG
                     AYDFMLISNLVRLAPVIKKCAQELKSIQENRDTQISLQKSEARFRAIASNLPGLVFQF
                     LKKPDKSVAFPFVSEGVLMLLGLTAEELTVQPDLFYQMILPEDQYSYNEQMKASAKNF
                     STWNWEGKIRVKGDSDIKWISLRATPRQSNQGGILWDGIVLNITRNKLAEIELANSHA
                     QLAELSSYLQKIKEHERMHIAREIHDDIGGTLTAIKCELQSCIDIDKPRLPEFYQQKA
                     RSIEELVDYVIDSTRRISLDLRPGVLDVGIVASVYWQAREFSKRNHIPCEVKCESEDI
                     SLNDDLAVAIFRIFQETLTNITKHANASIVKVKLAEINNHVLLEVIDNGKGITDLEMK
                     KPNSFGIRGIRERCQQLGGDFCITGTKGKGTSALIKIPIETKKIS"
     gene            4499..6208
                     /locus_tag="PKZ49_08165"
     CDS             4499..6208
                     /locus_tag="PKZ49_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HND36575.1"
                     /translation="MPQIINTNIASLNAQRNLNGSQGMLNTALERLSSGLRINSAKDD
                     AAGLAISERMSTQIRGLNQAARNANDGISLSQTAEGALTEIGNNLQRIRELSVQAANA
                     TNTASDRAALQAEVTQLTSEIQRVASQTQFNGLNLLDGSFSTMNFQVGANANQTISAS
                     SGNFQTNAFGNYRIGGLAAFTPGGSGDLVAGTIGTNSDAFGNSVLLSGTTGDTSGING
                     ALAAGDFRISTGSGNFDVYYRAGASANEIATAINRTGSGVNASATTEVVLGADTGAGA
                     GFQQNTTYSFAISTDYSDPTNANTVDPKYTTVSFKTGGADDTADVDSGSYLSTAVQAF
                     NDASAKTGFTAEAVLTEDGYWALKLTNSQGNDLRLLNNSYDNTGTALDITVSDTAVLD
                     GDTGTADSLADTLAGGGLDATTGVWTNGNGAWYTGKVTLDSAKSFTVTTAVADVFQDA
                     GTPGTAAAGTYGAQLQAASAMDITTAEAATRTLAIVDSALTTVNDQRARFGAIQNRLE
                     STISNLQTSSENLSASRSRIRDADFAAETASMTRAQILQQAGVAMLSQANAIPNNVLS
                     LLR"
     gene            6289..6651
                     /locus_tag="PKZ49_08170"
     CDS             6289..6651
                     /locus_tag="PKZ49_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HND36576.1"
                     /translation="MMISSTTGIADFLQPVLPQQKVQKNEHMPVQTTVTPESSQVNKQ
                     NTIELDVKEAVNQIQHFTETLAPNLQFSIDEETGKTVVKIMDAQTQEVIRQFPSEEAI
                     SIARALGKIEGLLFHDKA"
     gene            6684..8111
                     /gene="fliD"
                     /locus_tag="PKZ49_08175"
     CDS             6684..8111
                     /gene="fliD"
                     /locus_tag="PKZ49_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HND36577.1"
                     /translation="MISSPGLGSGLDVNGIVGQLMAIEQRPLLLLTNKEATQQAQISA
                     YGSLKGALSSFQSSVKTLTNLSIFTGIKANVGDSTIATASASTNAAVGSYQIEVQNLA
                     QAQKIKSESFATTSTAIGSGALTIEFGTYNGDGTFTSNPTKISKTITIESDQTDLASI
                     RDSINEADAGITASIVNDGSGNRLVFASNNTGTDNALKITVSDDDGNNLDNAGLSKLA
                     YDATTGGTANMTQTVAAQNATMVIDGITISKSSNTIKDALEGVTFNLLKAAPGTTTSL
                     SISEDTSKIQTAVSAFVGAYNELVKTISDISRYDTANKQASVLTGDATVRTVESQIRG
                     VLNSFLPGAPSSLNSLSQIGINFQKDGTLQLDNAKLTSVINDSEKDLGSLFATNGFAV
                     KLDKLVDGMLKNNGLIDGRLDGISDTIKDIGKQREMLGRRLEDVEKRYRTQFTALDTM
                     IASMTQTSTFLQQQLSNLPKASSGN"
     gene            8160..8612
                     /gene="fliS"
                     /locus_tag="PKZ49_08180"
     CDS             8160..8612
                     /gene="fliS"
                     /locus_tag="PKZ49_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HND36578.1"
                     /translation="MSAKNYAINSYQRVGVESGVASADSHKLILMLFEGSINALNRAK
                     QEILHNNISAKGEMISKAISIIDNGLKASLDISVGGEIANNLQALYDYMTRQLLIANI
                     QNSTGTINEVIELLSQLQEAWRSIGNKELAKPNIHQISNTHALEPTDI"
     gene            8620..>8944
                     /locus_tag="PKZ49_08185"
     CDS             8620..>8944
                     /locus_tag="PKZ49_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HND36579.1"
                     /translation="MDGVQVIFTYKAILTVTEKMLKAAQANNWEELISLEQECKLLTQ
                     ALVSNSNQIQLSDEMQKKKIDIIHRILDKDAKIRTITEPWMTHLQNLLINANQKRKLD
                     RLYSSD"
BASE COUNT         2797 a         1746 c         1678 g         2723 t
ORIGIN      
        1 tccattccaa gtaaagtatc tagaagcgag aaaataccaa ccataaaagc acgatcttga
       61 tatgttttat catgagggcg atcaatctgc gctataagct ccatcaattt tccacgaact
      121 gcagcagttt gcattaatgc gttcgaaatg ctatctcccg atgaatcaga ggtatataac
      181 aacaactgaa cccacctttg aagttgttgc cggcctaaaa ttattactgc ttgcttaatg
      241 gaattaattc tttgttgtaa gccaaaacca actgaattta ctaagcgtag taagttatag
      301 ctaagattgg gttgatgctt aagttctgtt tcaatctctt ctgcctcaca atcccccatc
      361 accatagtta ataaatttaa taatgggagc ttattcgcat caactcgctt acctgaaata
      421 atttcaggtt ttgcaaaatg atagccttga aacatatcga aaccgagagc cgtacatttc
      481 tttgccaact cagggctatc aaccttctct gctaacaaga taactggcca gcgtcttaat
      541 gtattgataa tttcttttag cttggtttca tcaattgcaa gcacatcgag ttttacaaca
      601 ttgacaatag gcaacatctg caatgttcta ctattgatct cagttacatc atcaagggca
      661 aggtggtaac ctatttgctt taactctaaa cagcgtttaa ctacttgatc ggtaatttct
      721 attgattcaa gtaattcgag tacgatatga ttacttggca gtaacagtat cgcgtcgctc
      781 atcagtaatt ctgtactaat gttaatgaag cctcgctgtt gaccaagtac tcgctgtatg
      841 cctagttgac cgtaagcatt aaccataaca ctggctgatg catgaacatc attggtaacg
      901 ttagcttcgt tgatttctcc agcacggaat aataattcaa atgccactaa attctggtta
      961 cgatccaata tgggttgcct acctaaaaat acttcctcca ttacgctact ccagaattga
     1021 acgtcatgta taaactagaa aaaatagcta caattatttg tgattgtggc taaaagtaaa
     1081 ataataaatt acgcaattat ttcaatacga tttttaccgt actgtttagc acggtaaagt
     1141 gctttatcgg cacgtttaat cacagtattc tgcgtttcct gaaacttacg cagtgtaatg
     1201 ccaccgctaa acgtaatttt aatcggtgtt tcttcagtat ctaacaaagg gttctttgat
     1261 aaattatttt gcaagcgctg cataacccaa atcgcttctt caggtctcgt atcaggcaat
     1321 atgatcagga actcttctcc gccatagcgt gcaattacat cacttgggcg tatcgttttt
     1381 ttagctatag ttgcgaaatg aatgagtgca ctgtctccaa ataaatggcc gtgagtatca
     1441 ttaatgaatt taaaatcatc cagatcaatt aaaacaacac agagcgcatt ttcataacga
     1501 tctgcgcgag cagcttcacg cataaaaata ttgtcgaggc cccggcgatt ataagtacca
     1561 gtcatttgat ctgtatgaac gagctccctg agctcctcta attcatattt taaagactcg
     1621 atcttatttt ctgcatggcg aagctcgcgg gtgttctgta tttcattatt attatgtaat
     1681 ccttttgttt cattaaggag tgtatctaag atcttgatca tttcatcaac atcattagtt
     1741 ttacgtattc tttgcgcaaa ctgctcaact tgctggtaat attcattcgc tgcagttaaa
     1801 gaattgctac ccccttcatt ctcaggcttg cgcataccta ctccagatgt attgattaat
     1861 cttagcaatt tttctttttt taagctcttc atatcgatga gacctcttag ctggcttcat
     1921 aaatgaaatt tccactctag ataagtatgg attgtttttt gaaggagaac ttacctagaa
     1981 tttatttctt cgtttagcag attagcaaat ctatgttgaa gtaagaaatg aaactaagga
     2041 tgaaaagacc ctttattctg tttttgcttt aacaatggtt ttagtcaact aatttatgtt
     2101 taattgcata acgaactaat tcagaattat tggaaatttt taatttttcc atcattcttg
     2161 cgcgataaac actgaccgtt tttggactta aacacatttc aatagagatc tcagagagtg
     2221 atttacctga tgcaatcaag cataaagtct gaaattcacg gtcagaaaga ttttcatgaa
     2281 gtggtcgatc cgcttctgaa gtccatgcat ttgctaattc ttgtgccaat gcaggagaga
     2341 tatatttatg tccttcagaa acttgtctaa ttgctgttac aagttgcgct ggcgcacttt
     2401 gcttatttaa atacccggaa gcgccagcct taagcgcacg taatgcaaat tcattctcat
     2461 tatgcatact aagcataagc accattaaat tgggtcggtc tttcttaatt tgctttaata
     2521 tttcgatacc attccgatca ggtaaagaaa tatctaacag catcacgtca aactcctgct
     2581 ggcgtatgac ttttaccgct tggaacccgg tttctgcttc ccctgttacg ctaatgtccc
     2641 ccgtttcact taaaatttgt tttaagcctt gacgtacgat cgcatgatcg tctgcaatca
     2701 ttactcgaat catctttatc ctttgcgact attgagtatc aagggttaac taattttctt
     2761 ggtttctata ggaatcttaa tcagcgcact agttcccttt cctttagtac ccgttataca
     2821 aaaatcaccg cccaattgtt ggcaacgttc acgaattccg cgaataccaa atgagtttgg
     2881 ctttttcatt tcgaggtcag taataccttt cccattatca ataacttcta ataaaacatg
     2941 attatttatc tcagcaagtt taactttaac aatagatgca tttgcatgtt ttgtaatatt
     3001 cgtcagagtt tcctggaata ttctaaagat tgctacggcc agatcatcgt tgagtgaaat
     3061 atcttcactc tcacatttca cctcacaagg aatatgatta cgtttactaa actcccttgc
     3121 ttgccagtaa accgatgcaa caatgccaac atctagaacg cctggcctaa gatctaatga
     3181 gattctacga gtactatcaa ttacatagtc aaccaactct tcaattgatc tagctttctg
     3241 ttgataaaat tctggtaacc taggtttatc gatatcaatg cacgattgga gctcacattt
     3301 gattgctgtt aatgttccgc caatgtcatc atgaatttca cgagcaatat gcatacgctc
     3361 atgttcttta attttttgta aataggaaga taattctgct agctgtgcat gtgaatttgc
     3421 aagttcaatt tctgctaatt tattacgagt aatattgagc acaattccgt cccatagaat
     3481 gcctccttga ttagattgcc gaggagttgc tcgcaaacta atccacttta tatcactatc
     3541 cccttttact ctaatctttc cttcccaatt ccaggtagaa aaatttttag ctgatgcttt
     3601 catttgctca ttatatgaat actggtcttc aggtagtatc atttgataaa acaaatcagg
     3661 ttgtacggtc aactcctcag ctgttaatcc tagtagcatc agcacccctt cacttacaaa
     3721 tggaaaagca accgatttgt cgggtttttt taagaattga aataccaaac ctggcaaatt
     3781 ggacgcaatc gcgcggaatc gtgcttcact cttttgaagg ctaatctgag tatcacgatt
     3841 ttcttgaata ctttttagtt cttgagcgca ctttttaata actggagcaa gcctaacaag
     3901 atttgaaatc agcataaaat catatgcccc agctttcaga aaatctttag ctgattgctc
     3961 attactttca ttcgatataa taattactgg aattctcaaa tattgattga cttgagaaaa
     4021 ataagtgagt attgcatgag tacctgtggg cattgtagct tcatttgcta tgatgatatc
     4081 ccagtgacac ttttgtattt catgtaagaa ctcatcataa gtgcccacca atttagaatc
     4141 aaaattaata tcctcatccg tgaatatctt acgaatagtc tgcgcactct gatttagaaa
     4201 acctactaag agtatagata gagactcact cacaataagt atttgttaat taatgaattg
     4261 caatcagcat aaccgaccat tatcaccatc gcaagtataa taattcatat tcatttgtta
     4321 taaatatata taagtcgcct atgactaatt aaacttttta ttagtcattc cgtttacctt
     4381 catcaacagc gactcatcat gacctgaata gattgcaggt agagtcaacg tcaccgtgag
     4441 cggcacgagc gccgcatttt tactgatcta tagtaatcaa actctgaagg agaaacaaat
     4501 gccacaaatt atcaatacta atattgcctc gttgaatgct caacgaaatt taaatggttc
     4561 acaagggatg ctgaatactg cgttagagcg tttatcatct ggcttacgta ttaacagtgc
     4621 taaagatgat gcagctggtc tcgcaatttc tgaaagaatg agcacccaaa ttagaggcct
     4681 taatcaggct gcgcgtaacg caaatgatgg catatcactt tctcagacag ctgaaggtgc
     4741 attaactgaa attggtaata acttacagcg tattcgtgaa ttgtcagtgc aagcagccaa
     4801 tgcgactaat accgcaagtg atcgtgcagc actccaggct gaggtaacac agctaacttc
     4861 agaaattcaa cgtgtagcta gtcaaactca atttaacggg ttgaacctgt tagatgggtc
     4921 attcagcaca atgaatttcc aagtcggtgc aaatgcaaat caaacgattt cagcttcctc
     4981 aggtaatttc caaactaatg catttggtaa ttatcgtatt ggtggcctag ctgctttcac
     5041 tcctggcgga tccggtgatt tagtggcagg aacaatcgga acaaatagcg atgccttcgg
     5101 taactccgta cttttaagtg gaacaacagg cgatacatcc ggcattaatg gtgctcttgc
     5161 agcgggagac ttcagaatct ctactggttc tggtaatttc gacgtttatt atcgtgctgg
     5221 tgcaagcgca aatgaaatcg caaccgcaat taaccgtacc ggtagcggtg taaatgcaag
     5281 tgcaacgact gaagtcgtat taggtgcaga tactggcgct ggtgctggtt tccaacaaaa
     5341 tacaacctac tcgttcgcaa tatcaaccga ttattccgat ccaacgaatg caaatacagt
     5401 agacccgaaa tatacgaccg tttcatttaa aaccggtggt gcggatgata cagcagatgt
     5461 tgattctggt agttatctga gtactgccgt gcaagccttt aatgatgcat ctgctaaaac
     5521 aggttttaca gcagaagcag tattaaccga ggatggttat tgggcgctta agctgacaaa
     5581 ttcgcaaggt aatgatcttc gtctcttgaa taattcatac gataataccg gaactgcttt
     5641 ggatattacg gtatcagata ctgcggtatt agatggcgat acaggtactg ctgatagttt
     5701 agctgataca ttagctggtg gcggtcttga tgcgaccact ggagtgtgga caaatggaaa
     5761 tggcgcttgg tataccggga aagtaacgct tgattctgca aagtcattta ctgtaaccac
     5821 tgctgttgct gatgtttttc aagacgctgg aacacctggc actgctgcag caggcaccta
     5881 tggtgctcaa ttgcaagcgg ctagcgcgat ggatattacg actgcggaag cagctactcg
     5941 tacactcgca attgttgact ctgccttgac aacagttaat gatcaacgtg cgcgctttgg
     6001 tgcgattcaa aatcgtcttg aaagcacgat tagcaatctg caaacttctt ctgagaattt
     6061 gtctgcctca cgcagtcgta tccgtgatgc ggattttgca gcagaaactg ctagcatgac
     6121 aagagcacaa attctacaac aagctggtgt tgcaatgctt tctcaagcaa atgcaatacc
     6181 taacaatgta ttgtctttat taagataagc aatagtattg ataatgcttg gtttagggta
     6241 gcagtgatca atgaactatc ctaaaccagt tatagagagg agtttaaaat gatgataagt
     6301 tcaactacgg gaatagcaga ttttttgcaa cccgtattac ctcagcagaa ggtacagaaa
     6361 aatgagcata tgcctgttca gactacggtc actcctgaat caagtcaagt aaataaacaa
     6421 aatacgattg aattggatgt aaaagaagca gtaaaccaaa tccagcattt tacagaaacg
     6481 ctagctccaa atttgcagtt ctcgattgac gaagaaactg gaaaaactgt agttaagatc
     6541 atggatgctc aaactcagga agtaatacgc caatttcctt ctgaggaagc gatttcgatt
     6601 gcacgtgcct taggaaaaat tgaagggcta ttgttccatg ataaggcata aatgtaacta
     6661 atagttgtat aaaggaaatt aaaatgattt cttcaccagg acttggttca ggattagatg
     6721 tcaatggaat tgttgggcaa cttatggcaa ttgaacagcg cccactttta ctacttacca
     6781 ataaagaagc tactcagcaa gcacaaattt ctgcctatgg aagcttaaaa ggagctttat
     6841 cttctttcca gagtagtgtt aagacattaa ccaatctatc gatatttact ggtattaagg
     6901 ctaatgtagg agattcaaca atagctactg caagtgcatc tactaatgca gcagtaggta
     6961 gttatcaaat agaagttcag aaccttgctc aggcccagaa aatcaaatct gaatcattcg
     7021 caactactag tacagcaata ggaagtggcg cactgacaat tgagtttggt acttataatg
     7081 gtgatggaac gtttactagc aatcctacaa aaatatccaa aactataaca atcgaatctg
     7141 atcaaacaga cttggcaagt attcgcgact cgattaatga agcagatgct ggaattacgg
     7201 caagcattgt caatgatgga agtgggaatc gtttagtatt cgcatcaaat aacaccggta
     7261 cagataatgc gctaaaaatt acagtgagtg atgatgatgg taataatcta gataatgcag
     7321 gattatctaa gttagcttac gatgcaacta cgggcggtac tgcaaatatg acacaaacag
     7381 tagctgcaca aaatgcaacg atggtgatag atggaataac tatcagcaag tcttcaaata
     7441 ctataaaaga cgccttagag ggggtaactt ttaatctgct aaaagctgct cctggtacta
     7501 ctacaagctt gtcaataagc gaggatacat caaaaatcca aactgctgtt agtgcatttg
     7561 ttggcgctta taatgagctt gtaaaaacaa ttagtgatat ctctcgctat gacacagcta
     7621 ataagcaagc atctgttcta acaggggatg cgacagttcg tacggttgaa agccaaattc
     7681 gtggggtgtt gaattcattc ttaccaggcg ccccaagtag cttaaattct ctttcacaga
     7741 ttgggataaa tttccagaaa gatggcacat tgcagttaga taatgccaag ttgacttcgg
     7801 taatcaatga ttctgaaaaa gatttaggct cattatttgc tactaacgga ttcgcagtta
     7861 agcttgataa attagttgat ggcatgctta agaacaatgg cttaattgac ggtcgcctag
     7921 atggaatcag tgatacgatt aaggatattg gcaaacagcg agaaatgcta ggccgccgct
     7981 tggaagatgt tgagaaaaga taccgcacac aatttacggc attggatact atgatcgcaa
     8041 gtatgactca aacaagcact ttccttcagc agcaattatc aaatctacca aaggcaagtt
     8101 ccggtaatta gtactttgat agataagtaa actttattaa ctatatataa gataaatcta
     8161 tgtctgcaaa aaattatgcc attaattcat atcagcgtgt tggtgtagag tctggagtag
     8221 catccgctga ttcacataaa ctcattctaa tgttgtttga agggtctatc aacgctctca
     8281 atagagctaa acaggaaatt ctgcacaata atatttcagc aaagggggag atgatttcaa
     8341 aagccatatc gattatcgat aacggcttaa aagcaagtct cgatatttct gttggtggtg
     8401 agattgcaaa caatctgcag gctctatatg actatatgac taggcagctt cttattgcaa
     8461 atattcaaaa tagtaccgga actattaatg aagttattga gctattgtcc caacttcagg
     8521 aagcatggcg gtctatagga aataaagaac ttgctaaacc aaatattcat caaattagta
     8581 atactcacgc tttagaacca acagatattt aaaaaattta tggatggagt acaagtaatt
     8641 tttacttaca aagcaatact aactgttact gaaaaaatgc taaaagctgc acaagcaaat
     8701 aattgggaag aattaatatc tcttgagcaa gaatgtaagt tattaacaca agctcttgta
     8761 tctaattcta atcagattca gctcagtgat gaaatgcaga aaaaaaaaat agatattatt
     8821 catagaatat tagataagga tgcaaaaatc cgaactatta ctgagccatg gatgacgcat
     8881 ctacagaatt tactgataaa tgcaaatcag aaacgtaaac tggatcggct gtatagctct
     8941 gatt
//
LOCUS       DAOQKQ010000109         8880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_3773_length_8880_cov_6.315014, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000109 DAOQKQ010000000
VERSION     DAOQKQ010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 8880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8880
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3773_length_8880_cov_6.315014"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(299..1900)
                     /locus_tag="PKZ49_08190"
     CDS             complement(299..1900)
                     /locus_tag="PKZ49_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HND36580.1"
                     /translation="MRLENAILEWKKIIGDSQVFDKETAEVEYGSCTTGVSRAISASI
                     KPLDASHIPNIVKIANKYKVALYPISTGHNWGYGTALPSIDNCVILDLSALNKILEFD
                     PDTGLVTLEPGVTQGQLAEFLTLGNHSYMVPVTGAGPTCSLLGNALERGYGITPITDH
                     FGAVISIEAVLADGSIYQSALSAMGGESIDRAFKWNVGPYLDGIFSQGSFGVVTKMTI
                     ALAHRPESVESFLFGLKNSEQLPDLVRLIQKIITRYPGILGGVNLMNAHRVLAMSIPY
                     PRDKLALNKTIAQETLSELCKANHVMPWTGLGTLYGSRKVLKVVKQEIKAMLSPIASQ
                     LIFVNSGLIKTSIKVINFFPNFIRTKFERKLMMLERSMQLVSGWPNQTALPLCYWLMN
                     DSDVRQEVFLNPARDGCGLIWYSPLVLMKPDSVMQYINMVTSVMQKHGLEPLVTLTSV
                     TNACFDSTVPLLFDRTSSQGQKSAENCYWELLETGKKLGFVPYRFGIHAMSWLTADET
                     PYWQTVRKIKRSLDPDNIISPGRYV"
     gene            2066..3307
                     /locus_tag="PKZ49_08195"
     CDS             2066..3307
                     /locus_tag="PKZ49_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HND36581.1"
                     /translation="MNSNWEKSIEISLAFRNIIRQKQRSLISLGAIIFGVCSLILASG
                     FIEWIFIDFRETSIRSQLGHLQITRPGYQEEGKANPYHFLLPDTQPELKLTSSSLEKI
                     KVIVPRLSFSGLISSGESTLSFIGEGVDPNEQNFFGDALQLTTGNYLSGNNTFQIIMG
                     EGLSRNLGVETGDNVTLLVNTASGGINAIEVTISGLFTTVTKSYDDNAIRIPIETAQK
                     LIRVTGAHSWIVLLNHTEDTNQVLSLLHDQLPQSKFEITPWYQLADFYNKTTVLLSKQ
                     VLAIKIIIAIIILLSISNTMSISVIERTGEIGTAMALGIKQTKILYMFLTEGILLGCI
                     GGILGVMIGYLLANIISSVGIPMPPPPGMARGYIGEILVTQSMVLEAISLAIGTTLIA
                     SVYPAWKASKLQIVDALRHNR"
     gene            3314..4534
                     /locus_tag="PKZ49_08200"
     CDS             3314..4534
                     /locus_tag="PKZ49_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HND36582.1"
                     /translation="MLKLSLRNILRQKIHTTMSLISIICGVVGIILAGGWVNDIFIQL
                     GEALIHSQSGHIQIYKQGYFYEGSRSPEKYLIYDFDQMKKTVAQQITVEKFDSITARL
                     NFSGLLNNGRSDLPIVGEGIHPENEGQFSTALKITDGRQLTDQDKFGLMLGRGLANGL
                     KLKPGDTVTLLTNTIEGTLNSLDFEVIGIFQTFSNDYDARTIKISLATAQELLSINAV
                     NALVISLKETKDTDQITALLKNKLDSFDLEVRTWTELNDFYEKTVDLYKSQFGVLQLI
                     ILVMVCLSVANSVNMSIYERTGEFGTMLALGNRSMDVFYLIISENLLLGCIGASLGIA
                     IGMFLALTISAIGIPMPPPPNADLGYIAQIRIVPSVLLLAFLIGLFATVIAAIMPARK
                     IAQLPIVEALRQNY"
     gene            complement(4535..5719)
                     /locus_tag="PKZ49_08205"
     CDS             complement(4535..5719)
                     /locus_tag="PKZ49_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36583.1"
                     /translation="MAFAYADPDHLSKMRLRTELENIRQINSFIKWKLSGRIDYDAVY
                     SISNFYSHNVREDQRFEFWLRENYLDISRGNWDFRVGRQHIVWGEMVGLFFADVVSAK
                     DMREFVLQDFNLLRIPQWALRTEYSKNNFHADLVWIPFATLDKIGRPGEPGRSAAEFY
                     PFALPDQLQATFLNEDRSGRNITNSNYGLRLSQLVNGWDFSAFYYHSLDASPTFYLVG
                     GELGRPVFQARHDRIDQVGSTFSKDLGSVVVKGEFVYTNGRQFNVTRFSQPFGLVQQD
                     TVDYALGLDFSLPAEIRTNLQFFQRVFLSYDRDIFHDKWESGASIMLNRKLWQDVEAQ
                     TLLIHSLNRVEWMLRPRINWNFTKNWRLALGADMFEGPATGLFGRFANNDRIFTEIRL
                     SF"
     gene            complement(5981..6682)
                     /locus_tag="PKZ49_08210"
     CDS             complement(5981..6682)
                     /locus_tag="PKZ49_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HND36584.1"
                     /translation="MNIVQISNVSKEYMLGEQHVQALKDVNLEIEESVFLAIAGPSGS
                     GKSTLLNLIGCIDNPTTGKIFIDGQDTSGKTPDELSELRLRNIGFIFQTFNLFPVLSA
                     EENVEYPLLQIKELDSDERQERIVKFLDIVGLSKFAHHRPNQLSGGQRQRVAIARALA
                     THPKIILADEPTANLDHNTGEGILELMKEINRKDKTTFIFSTHDSRVMSMADRLIHIE
                     DGRIIRENTVNSMGS"
     gene            6734..7507
                     /locus_tag="PKZ49_08215"
     CDS             6734..7507
                     /locus_tag="PKZ49_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HND36585.1"
                     /translation="MTNMSQQYFMNYLLFLYFTLIPIAVFANTTEDTDTLARAILEKA
                     DAIRFPRESFQVEVDIKTISPEKINEEYKYRVLSKGNENSIVMITEPASERGQTILMK
                     ERDLWVFMPSVSQPIRLSLSQRLVGQVANGDIARANFSGDYNPKLLRTEIINDKAYYV
                     LELTAVDRSVTYHKVVLWVQESNYWPYRAEFYSLSNRLLKTSRYENFDWVLGIERPTR
                     LIMEDALKKDEVSILDYSEMILRDLPDKIFTKEYLKKLD"
     gene            7565..8386
                     /locus_tag="PKZ49_08220"
     CDS             7565..8386
                     /locus_tag="PKZ49_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM/exosortase system-associated
                     acyltransferase"
                     /protein_id="HND36586.1"
                     /translation="MSQNEIINIGATFSEHFEIVPALSEELKNEAFRVRHQVYCEDLN
                     YESQRASKYETDEYDINALHLLMKSVRLNKFIGCTRLIRPSIDDPHQPLPFEKCCGNT
                     LDHSKVGSLLLSRNKIAEVSRLAVISEFRRRKGESERPINLSDEDYGISNYARFRFPY
                     IPLGLFIGTVELAYIHHIDILFMLTEKRLAMHFSKLGANLDFIGMPVEHRGVRVPSMV
                     NTVEIIKNMKPMFRPLYNLISKTICESLSRSTQNPFIIQIGSDPQNTANQQLNRL"
     gene            8515..8601
                     /locus_tag="PKZ49_08225"
     tRNA            8515..8601
                     /locus_tag="PKZ49_08225"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8549..8551,aa:Leu,seq:caa)
BASE COUNT         2775 a         1830 c         1592 g         2683 t
ORIGIN      
        1 caccaaccac aatcgcttga gctgacatag aagcaagaaa accacctccc actatcgccg
       61 caatcgctgt tttttttaat tgtttaatca ttcgaatctc cttagttttc ctatcactga
      121 gttatgaccg acaaattatc tgccatcaca aaaagaaaag caacttgtat gccaaaatct
      181 taataaatta ataaatcaca accagaacaa tgcattacaa cagcagcagc aaccaatcca
      241 aattttagaa aaataaaagt gtaagaaaaa tcgacagaat tacctagaga aaataagatt
      301 aaacatacct acctggcgaa ataatattgt caggatcaag tgatcgttta attttacgaa
      361 cggtttgcca atagggcgtt tcatcagcag tcaaccagct catcgcatga ataccaaatc
      421 ggtaaggaac aaacccaagc ttcttccccg tttctaataa ctcccaataa cagttttctg
      481 cactcttttg accttgactc gatgtacgat caaataacaa gggaactgtg ctatcaaagc
      541 aagcgtttgt cacacttgtc aaagtaacga gtggctctaa tccatgcttt tgcatgacgg
      601 aagtcaccat gtttatatac tgcatgacac tgtcaggctt cataaggact aatggtgaat
      661 accaaattaa tccacatcca tccctggcag gattcagaaa aacttcttgc ctaacatctg
      721 aatcattcat taaccaatag cacaggggca atgcagtctg attaggccaa cccgaaacca
      781 attgcattga acgctctaac atcattaatt ttctttcgaa cttggtccga ataaagtttg
      841 gaaagaaatt tatgacttta atcgaagttt ttatcaaacc agaatttaca aaaatcaatt
      901 gtgatgctat tgggcttaac attgctttta tttcttgttt aaccaccttc aatacttttc
      961 tcgacccata aagtgtccct agacccgtcc aaggcattac atgattagcc ttacaaagtt
     1021 cagaaagtgt ttcttgtgca atagtcttat ttagtgctaa tttatctcga gggtacggaa
     1081 tgctcatcgc caagacgcga tgagcattca ttaaattcac accacctaaa atacctggat
     1141 aacgtgtaat aattttttga ataagtctta ccaagtccgg taattgctca gaatttttaa
     1201 ggccaaacaa gaatgactcc acggattctg ggcgatgtgc aagtgcaatg gtcatttttg
     1261 tcacaacacc aaaacttcct tgtgagaaaa ttccatctaa gtaaggtcca acattccatt
     1321 taaaggcgcg gtcaattgat tctcctccca tagcagagag ggcagattga taaatactac
     1381 catccgcaag tactgcttca atagaaatta ctgcaccaaa atgatctgtt atcggagtga
     1441 ttccatagcc tcgttctaag gcgttcccaa gtaagctgca cgttggccca gcacctgtta
     1501 caggaaccat ataagagtga tttccaaggg taagaaattc cgctagttgc ccttgagtga
     1561 ctccgggttc taacgttacc aaaccagtgt ctgggtcaaa ctccagaatt ttgtttagtg
     1621 ctgataaatc taaaattaca caattgtcaa ttgatgggag cgctgttccg taaccccagt
     1681 tatgtccagt actgatggga taaagtgcca ccttatactt attcgcaatc ttaacgatat
     1741 ttggaatatg gctagcatca agtggcttaa tactagcaga gatggcacgg cttactcctg
     1801 ttgtacatga accatattca acctcagcag tctccttatc aaaaacctga gagtcaccga
     1861 taattttctt ccattctaaa atcgcatttt ccaatctcac cagctggcat cctgataaag
     1921 aaaaaaatcc cgttttacgc caaaattaac tttaccatct agtttatttg ttttttcacc
     1981 cattaatcaa cacaaagata tagtacatct tggttattat atcgttatct tttttcataa
     2041 ccatattaat aactcttata ctagaatgaa cagtaattgg gagaaatcca tcgaaataag
     2101 cctagcattt cgcaatatca ttcgccaaaa acaacgaagt ttaatttcat taggggccat
     2161 catatttggc gtttgttcac tcattttagc aagtggtttt attgagtgga tttttatcga
     2221 cttccgggaa acttcgattc gatctcagtt aggtcattta caaatcacac gtcccggtta
     2281 tcaagaagaa ggtaaagcaa atccttatca ttttctatta cctgatacac aaccagaatt
     2341 aaagctcaca tcaagtagct tagagaaaat taaagttatt gtcccacgct tatcttttag
     2401 cgggttaatc agcagtggcg aatcgacgct atcgtttatt ggcgaagggg ttgatccaaa
     2461 cgagcagaac ttctttggtg acgcacttca actcactacc ggaaattatt tgtctggaaa
     2521 taatacattc cagattatta tgggagaagg tttatcgcgc aacctgggcg tagaaactgg
     2581 agataacgtt acattactgg taaatacagc ctctggagga attaatgcga ttgaagtaac
     2641 cattagcggg ctttttacaa ctgtcacaaa atcatacgac gataatgcaa ttcgaattcc
     2701 tatcgagaca gcccaaaaac tcattcgtgt gacaggcgca catagttgga tcgttttgct
     2761 taatcatact gaagatacaa atcaagtcct ttctttactg cacgatcagc ttccccaaag
     2821 taagtttgag attacacctt ggtatcagct tgctgacttc tataataaaa caaccgtgct
     2881 cctttctaaa caagtgctag caatcaaaat cattatcgca ataatcattt tgttaagtat
     2941 ttccaacacg atgagtatta gtgtgataga acgaaccgga gaaatcggta cagcaatggc
     3001 acttggaatc aaacaaacaa aaattctgta catgttccta acggaaggta tcctgcttgg
     3061 atgcatcggt ggcatacttg gtgtaatgat cggttattta ttagcgaata ttatttcaag
     3121 tgtcgggata ccaatgcccc ctccccccgg catggcaaga ggatacatag gggaaatatt
     3181 agttactcaa tcaatggtat tagaagctat atcattagcg ataggaacaa ctcttattgc
     3241 aagtgtttat ccggcttgga aagcttcaaa attgcaaatc gtcgatgcgc ttcgccataa
     3301 ccgataaaaa tcaatgctta aacttagctt acgcaacatt ctgagacaaa agattcatac
     3361 cacaatgagt ttaatctcga ttatttgtgg tgttgttgga atcattttgg ctggtgggtg
     3421 ggttaatgat atttttatcc aactaggtga ggctctgatt cactcacaat ctgggcatat
     3481 tcaaatatat aagcaagggt atttttatga aggttctcgc tcccctgaga aatatcttat
     3541 ttatgatttt gatcaaatga aaaaaactgt tgctcagcaa ataactgtcg aaaaatttga
     3601 cagtattaca gctcggctaa atttttctgg cttattaaat aatggtcgta gtgacctacc
     3661 aatcgttggt gaaggcattc accctgaaaa tgaaggtcag tttagtactg cactaaaaat
     3721 cactgacgga agacagttaa ctgaccagga taaatttggc cttatgctcg ggcgtgggtt
     3781 agccaatgga ctaaaactta aaccaggtga tacggttacc ttacttacaa atacaattga
     3841 aggcactttg aacagcttag attttgaagt aatcggtata ttccaaacct tttcaaatga
     3901 ttatgacgcg cgcaccatca aaatttcgct agcaacagcg caggaactcc tgagtattaa
     3961 tgccgtaaat gcgctcgtga tttcactaaa agaaactaaa gacacggatc aaataactgc
     4021 tttactaaaa aacaagctag attcctttga tcttgaagtt agaacttgga ctgaactcaa
     4081 tgatttttat gagaaaacag ttgatttgta taaaagtcaa tttggtgtac tgcaattgat
     4141 cattcttgta atggtatgtt taagtgtcgc caatagtgtc aatatgagta tctatgaaag
     4201 aaccggggag tttggcacga tgctggcttt aggtaatcgc agtatggatg ttttctactt
     4261 gatcatcagt gagaacctct tattaggatg catcggcgct agcctcggta ttgcaatagg
     4321 aatgtttctt gcgctaacga tctccgccat cggcataccc atgcctcctc cacccaatgc
     4381 cgatctaggt tatattgctc aaatcaggat tgtaccttct gttctattgc ttgctttttt
     4441 gatcggatta tttgctaccg tgattgcggc cattatgccc gcaagaaaaa ttgctcagtt
     4501 accgattgtt gaggcacttc gtcaaaatta ttgatcagaa tgaaagacga atctctgtaa
     4561 aaatacgatc attatttgca aaacgaccaa aaagccctgt tgctggtcct tcaaacatat
     4621 cagctcccaa tgcaagtcgc caattttttg tgaaattcca attaattctg ggtctaagca
     4681 tccattctac tcgattcagg ctatgaatca gtaacgtctg cgcttcaaca tcctgccata
     4741 attttcgatt cagcatgatg ctcgcgccac tttcccactt atcatggaaa atatcccggt
     4801 catagctaag aaaaactcgc tgaaaaaact gtaggttggt tctgatctct gcaggcaggc
     4861 taaaatcgag ccctaatgca taatcaacag tatcttgttg aactaaccca aacggttgtg
     4921 agaatcgcgt tacattaaat tgcctaccgt tggtataaac aaactcacct ttgaccacca
     4981 ctgagcctaa atctttagaa aatgtactgc cgacttgatc aattcgatca tgacgcgcct
     5041 gaaatacagg tctaccaagc tcaccaccaa caagatagaa agtaggtgat gcatcaaggc
     5101 tatgataata aaacgctgaa aaatcccacc cattaacgag ttgtgataag cgcaaaccat
     5161 agtttgaatt tgtaatatta cgacccgaac gatcttcgtt tagaaatgtt gcttgtaatt
     5221 gatcgggaag tgcgaaaggg tagaactcag ctgctgacct acctggctca cccggcctac
     5281 caatcttatc cagcgttgca aaaggaatcc aaaccagatc cgcgtgaaag ttattttttg
     5341 aatattcggt ccgcaatgcc cactgcggga ttctaagcag gttaaaatct tgtagcacaa
     5401 attcccgcat atcctttgct gataccacat cagcaaagaa taaaccgacc atttctcccc
     5461 aaacaatatg ttgtcggccg acacgaaaat cccaattgcc tcgagaaata tctaaataat
     5521 tttcgcgtaa ccagaactca aatcgctgat cttctcgcac attgtgtgag taaaaatttg
     5581 agatactata aactgcatcg taatcaatcc gaccacttaa tttccattta atgaacgaat
     5641 tgatttgtcg aatgttctca agctcagtcc gtaagcgcat tttggacaag tgatcaggat
     5701 ctgcgtacgc aaatgccatt tcatattgtg agaaaccccg ccagcttgat gtttgtttga
     5761 aacgttgatt agagactttc gttgaagatg ttttattatc tataaataaa tcatctagag
     5821 atgcaaccct atttgagcct acgcttttaa tctcttgctt aattgataga atattcgttt
     5881 tatcagaacc aaagcaatat gagatgcttg caaagcatat tacgcttgtt attgttaaac
     5941 aggttccaat cgtgttataa atctgcgtaa aagacacctg ctaacttccc atgctattaa
     6001 cagtattttc tctaataatt ctaccgtctt ctatatgaat taaccgatct gccatggaca
     6061 taacacgaga atcatgggtt gaaaaaataa aagtagtttt atcttttctg ttaatttctt
     6121 tcattaattc caagattcct tcacctgtat tatgatcaag attagcagta ggctcatctg
     6181 ccaggataat tttaggatga gtagctaaag cacgtgcaat tgctactctt tgacgttgcc
     6241 cgccgctcaa ttgattagga cgatgatgag caaatttgga caaaccaact atgtcgagaa
     6301 attttacaat cctttcctgt cgttcatcag aatctaattc tttaatttga agtaaggggt
     6361 attccacgtt ctcttcagca gataaaacgg gaaataggtt aaatgtctga aaaataaaac
     6421 cgatatttct taatctaagt tctgacaatt catccggtgt ttttccactt gtatcctggc
     6481 catctataaa aatttttcca gtcgttggat tatctatgca accaattaaa ttaagtagag
     6541 tagacttacc gctccctgat gggcccgcaa ttgctaaaaa aacactttcc tcaatctcta
     6601 gattaacatc cttaagcgct tggacatgtt gctcgcccaa catgtattcc ttagatacgt
     6661 tactaatttg aacaatattc atgagacttg attccaggtt gttttcacag aaacgatatt
     6721 ttactggaaa gagattacta atatgagcca acaatatttt atgaattacc ttttatttct
     6781 ttatttcacg ctaattccaa tagctgtctt tgcaaataca acagaagaca cagataccct
     6841 agccagagcc atattagaaa aagctgatgc aataagattt ccccgagaaa gctttcaagt
     6901 cgaagttgat attaagacta tctctcctga aaaaataaat gaagaatata aatatcgtgt
     6961 gttatctaaa ggcaatgaaa atagtattgt catgataact gagccggcat ctgagcgtgg
     7021 ccaaactata ttaatgaaag aacgtgattt gtgggtattc atgccaagtg tttcccaacc
     7081 tatccgtcta tcgctttccc agcgtttggt tggtcaagtt gcaaatggtg atattgctcg
     7141 tgcaaacttc tctggagatt ataaccccaa gcttctacgt acagaaatta tcaatgacaa
     7201 agcatattat gtacttgagt tgaccgctgt cgatcggagc gttacttacc acaaagttgt
     7261 tctctgggtg caagaatcca attactggcc ttatcgagca gaattctatt cattatcaaa
     7321 tcgccttctt aaaaccagcc gctatgaaaa ctttgattgg gtattgggca ttgaacgccc
     7381 gactcgcctt attatggagg atgcgctaaa aaaggacgag gtttcaatac tcgattattc
     7441 cgaaatgatt ttgagagatc taccggacaa aatattcaca aaggaatatt taaaaaagct
     7501 tgattaattt taaagccaac cccaaggctt ccaaatcgca aaaactatta tagggcttcg
     7561 atctatgtca caaaatgaaa ttattaatat tggcgcaact tttagcgaac attttgaaat
     7621 tgtacccgca ttatctgaag aattaaaaaa tgaagctttt cgtgtacgcc atcaggtcta
     7681 ttgtgaagat ttaaactatg aatcccagcg tgctagtaaa tatgaaactg atgagtatga
     7741 tatcaacgcc cttcatctct tgatgaaaag tgttaggtta aataaattca ttggatgtac
     7801 aagattaatc cgaccatcaa tcgatgatcc gcatcaacca cttccatttg agaaatgctg
     7861 tggaaatacc ttagatcact ctaaagtcgg ctcattactc ttatctcgca ataaaattgc
     7921 tgaagtatcc aggctcgctg taatttcaga atttcgtcgt cgtaagggag aatcggaaag
     7981 acccatcaat ctttctgatg aagactatgg tatttcaaat tatgcgcgct tccgctttcc
     8041 gtacattcca cttggccttt ttattggaac agtagaactt gcttatattc accatattga
     8101 tattcttttt atgctaactg aaaaaagatt agcaatgcac tttagtaaat tgggcgcaaa
     8161 tcttgatttt attggtatgc cagttgaaca tcggggtgtt cgcgtacctt ctatggttaa
     8221 tactgtcgaa ataattaaaa acatgaagcc aatgtttagg ccattgtata atctgatctc
     8281 aaagacaatt tgcgaaagtc tctcgcgatc gactcaaaat cctttcatta ttcaaatagg
     8341 ctctgatccc cagaacactg caaatcagca actcaatcgt ttatagaaga taaagctgat
     8401 acataaagta gttaaattaa aggtaatgta tacacttact gaaataacag gattgctgag
     8461 acctattatt tcagtaataa tgtgcacttg ctggatttaa ggttatacat ataagccgag
     8521 atggcggaat tggtagacgc acgggactca aaatcccgcg atggtgacat catgggggtt
     8581 cgattccccc tctcggcacc aaatgagtat ttcaagcaat aaggttgtat attttcttat
     8641 aaaatcttgc cccatcttga cttctgtcaa ctcgcttgca gcttctgcta accgctagcg
     8701 ccaatcgatt ttctttaaaa aaacttaatc agttgctcac cggattcttg gttatacttc
     8761 gtcggcgata atcgtttcgt catgtcccct atttcgcgtc tttattttac gaaaattgga
     8821 ttctttgaaa ttcagcatac ttaagtctta tctgctgctt ttttaccaat acgcgtttcc
//
LOCUS       DAOQKQ010000110         8828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_3807_length_8828_cov_5.445572, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000110 DAOQKQ010000000
VERSION     DAOQKQ010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 8828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8828
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3807_length_8828_cov_5.445572"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..1999)
                     /gene="rpoD"
                     /locus_tag="PKZ49_08230"
     CDS             complement(101..1999)
                     /gene="rpoD"
                     /locus_tag="PKZ49_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HND36587.1"
                     /translation="MVDTPVTPEVVAPTAQDIEAKKTRLKKLIVQGKERGYLTYAEIN
                     DHLPDDMLDAEQIEGVISMINDMGISVHDEAPDAEALLMSDTATAVTDEDVVEEAEAA
                     LSNVDSEFGRTTDPVRMYMREMGSVELLTRESEIEIAKRIEDGLRHMIQAISSSPGII
                     GDILNLATEVENDRLRVDDLVDGFLDDDSQEMMSEEISDESMEQDFISDEMDEEGNVA
                     VVSASLLKLKADVLERFVKIRKAYNEMQKIVEKTGSRDGKKYQTLQEAISTELMVMRF
                     SAKMVEKLCDKQRDLVNEIRSCERKIMELCVSKAGMPRNHFIKSFPGNETDLDWIDGE
                     IKANKAYSQSLNHFMPAIKEQQQNLLALQELAMIPIKELKEINRQMTTGEAKARRAKR
                     EMTEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWI
                     RQAITRSIADQARTIRIPVHMIETINKMNRISRQILQETGQEPEPALLAQKMEMPEEK
                     IRKILKISKEPISMETPVGDDEDSHLGDLIEDSSTMAPADAAVYASLRIATKDVLDSL
                     TPREAKVLRMRFGIEMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPARSDRLRS
                     FLDTGGNP"
     gene            complement(2416..4295)
                     /gene="dnaG"
                     /locus_tag="PKZ49_08235"
                     /pseudo
     CDS             complement(2416..4295)
                     /gene="dnaG"
                     /locus_tag="PKZ49_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110873.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
     gene            complement(4384..4836)
                     /locus_tag="PKZ49_08240"
     CDS             complement(4384..4836)
                     /locus_tag="PKZ49_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HND36588.1"
                     /translation="MDLKQKIADDMKAAMRSGDSKRRDAIRLLQAAIKQREVDERITL
                     DNVAITAVIEKMLKQRKDSITQYEMAKRQDLAEAEKFEIEVLQTYMPQPLSEEEIIQI
                     INEIIASVGQLDQQSMGKVMAALKPKLAGRADMGYVSGLIKEKIKALT"
     gene            complement(4867..5079)
                     /gene="rpsU"
                     /locus_tag="PKZ49_08245"
     CDS             complement(4867..5079)
                     /gene="rpsU"
                     /locus_tag="PKZ49_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HND36589.1"
                     /translation="MTTVKVKENEPFEVAMRRFKRAVEKTGLLTELRAREFYEKPTAE
                     RKRKLAAAVKRTYKRLRSQLLPPKLY"
     gene            complement(5232..5843)
                     /locus_tag="PKZ49_08250"
     CDS             complement(5232..5843)
                     /locus_tag="PKZ49_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36590.1"
                     /translation="MGILSIIIFALCVGLLAGAGAGNDHILPITLITAVVLIASRDKI
                     AARSNAVSDELSNSDLQSNHHLENYYTWPELNQFQYEVKSNSYQGAINQLLKENISPP
                     AAVSIDVPLILNAHLIPDSDHLYDKNAVRIDIHDRTIGYLSPEEALIFYQRLSQQGLT
                     NQVTMCRVLIDKKRDKVNKTNIYKVKLDIEPFLVPEKSNIKSE"
     gene            5924..6343
                     /locus_tag="PKZ49_08255"
     CDS             5924..6343
                     /locus_tag="PKZ49_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HND36591.1"
                     /translation="MDEESSLIALFTSSFLAATLLPGGSEAVLIGVLMAYPELFTSAL
                     SVATIGNTLGGMSSYLIGRFLPDEKAILKKTNNSLRSLEWIHRHGSPILLLSWLPIIG
                     DALCVAAGWLRIHWFYAILFIAVGKLARYWVIATAIS"
     gene            complement(6372..6680)
                     /gene="grxD"
                     /locus_tag="PKZ49_08260"
     CDS             complement(6372..6680)
                     /gene="grxD"
                     /locus_tag="PKZ49_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HND36592.1"
                     /translation="MTTSESIEQLISSNPVVLYMKGTPSQPQCGFSANAVKILNACGV
                     TNFVAVDVLSDPEIRQGIKDFSNWPTIPQLYVNGEFVGGSDIMTEMYQSGELKKLLEK
                     "
     gene            complement(6827..7651)
                     /gene="prmC"
                     /locus_tag="PKZ49_08265"
     CDS             complement(6827..7651)
                     /gene="prmC"
                     /locus_tag="PKZ49_08265"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HND36593.1"
                     /translation="MPVTIAQLLNDAKYHVSDVDARLLLQHALQVSPVFLAAYSDEFV
                     NAEKMMCFYSLLQRRINGEPIAYIIGQREFYDLTFHVTPAVLIPRPETETLVELALAS
                     IPMDRSYTVLDLGTGSGAIALTLAKHRPLAQVMAIDLSAEALEVAQMNATLLEVDNVH
                     FTLGDWFQGIVGQYDLIVSNPPYVAIGDPHLDQGDLRFEPQIALSAPEDGIACIRTII
                     AQAPQFLCAGGQLLLEHGYNQAAQCRQLLAQEASFINIFSYPDLAGILRITGGQRI"
     gene            complement(7745..8824)
                     /gene="prfA"
                     /locus_tag="PKZ49_08270"
     CDS             complement(7745..8824)
                     /gene="prfA"
                     /locus_tag="PKZ49_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HND36594.1"
                     /translation="MNKSIAERLTRLSVRLEELNQLLSSEKVTTDLDNYRKITRERSE
                     IIPVVELYHAHQQTELDIQTAKEMSADPHLRDFAEAELITGKEKLQQIETELLKQLLP
                     KDPSDERNIFLEIRAGTGGDESALFAGNLFRMYARFAERQGWQVEIISQNPSDVGGYK
                     EIIARIIGYGAYSRLKFESGGHRVQRVPVTETQGRVHTSTCTVAVIPEADEIASVELN
                     PAELRIDTFRASGAGGQHINKTDSAVRITHLPTGIVVECQEGRSQHKNKAQAMSVLAA
                     RILDKQTREQQAEQAATRKSLVGTGERSERIRTYNFPQGRVTDHRINLTLYKMDQIMD
                     GDIGELCSALMAEHQAAQLESLMER"
BASE COUNT         2535 a         1982 c         1520 g         2791 t
ORIGIN      
        1 cgaccaaggg attatgagtc ccctgctcta accaactgag ctacagaccc ttatttactt
       61 agctaaactc tatagaaccc aatttaccag gcaaagcaaa ttacggattt ccacctgtat
      121 caagaaagct tcgaagtcga tcagatcggg cagggtgtcg cagttttcgc aatgcttttg
      181 cctcgatttg tcgaatccgt tcccgcgtaa catcaaactg ttttcctact tcctccaacg
      241 tatgatccgt attcatctct attccaaatc gcatgcgcaa tacttttgct tctcttggag
      301 taagtgaatc taatacatct ttggtcgcta ttctcaaact tgcataaacg gcagcatctg
      361 ctggtgccat tgttgatgaa tcttcaatga gatcaccaag atgagaatcc tcatcatcac
      421 caactggcgt ttccattgaa ataggttctt ttgaaatctt taagattttt ctaatctttt
      481 cttctggcat ctccattttc tgagctaata gtgctggttc tggctcttga ccagtttctt
      541 gcaaaatttg ccgtgaaata cgattcatct tattgatagt ctctatcata tgcactggaa
      601 tccgaattgt tcgtgcttga tcggcaattg atcgagtaat cgcttgccga atccaccatg
      661 tcgcataagt tgagaattta taaccacggc ggtattcaaa tttatctact gccttcatta
      721 aacctatatt accttcttgg atcaaatcga ggaattgtag gccacgattg gtatatttct
      781 tggcaatcga aataaccaaa cgcaagttag cttcagtcat ctctcgttta gctcgacgag
      841 ctttggcttc gccagtagtc atttggcgat taatttcttt caattccttg attggaatca
      901 tcgccaactc ctggagagca agaagatttt gctgctgctc tttgatagcc ggcataaaat
      961 gatttaatga ctgactgtaa gctttgtttg ccttaatctc accatcaatc caatctaaat
     1021 ctgtttcgtt tcccggaaac gatttgatga aatgattcct cggcatccca gcttttgaaa
     1081 cacaaagctc catgatcttt ctctcacagc tacgaatttc attgacgaga tcacgctgct
     1141 tatcgcataa cttttcgacc attttggcag aaaatctcat caccattaat tcagtagaaa
     1201 tagcttcctg gagcgtctga tatttcttac catcacgcga tccagttttt tctacaattt
     1261 tttgcatttc attataggct ttacgaatct tgacaaagcg ctctagcaca tcggctttta
     1321 gcttaagtag gctggcactg acaaccgcaa cattaccttc ttcatccatt tcgtcagata
     1381 taaaatcttg ctccatcgat tcatcagata tttcctcact catcatctct tgtgagtcat
     1441 catcaaggaa gccatctact aaatcatcta cgcgtaatcg atcattctcc acttctgtag
     1501 ccaaattaag gatatcgcca ataattccag ggctggaaga aatcgcctga atcatatgcc
     1561 ttaatccatc ttcgatgcgc ttggcaattt cgatttcgct ttcacgtgtc agcaactcta
     1621 ccgaacccat ttcccgcata tacattcgaa caggatccgt tgtacgacca aattctgagt
     1681 caacatttga aagcgccgct tcagcttctt caaccacatc ttcatcggta acagctgtag
     1741 ctgtatcaga catgagcaat gcttcagcat ctggtgcctc atcatgaact gagataccca
     1801 tatcattaat catgctgatc acaccttcaa tttgctctgc gtcaagcata tcatcaggaa
     1861 ggtgatcatt aatctcggca taagttaaat aaccacgttc tttgccttga acaatcaatt
     1921 ttttaaggcg tgtttttttg gcttcaatat cctgagccgt aggcgcaaca acttcaggag
     1981 taacaggtgt atcaaccatc tccttggagg caaccgcagc ttttagtttc tttgctactt
     2041 tattattttt tgatgacttt gcgcccttca taacctcatt ttcctctaac gactgctctt
     2101 cagtaatttc tagctgctca gtagtgggcg aatttttagt agctttttct cgcttcgttg
     2161 gtttattact catgttatta tctttgggca tcttcgtgat aatcactcaa aattgttatt
     2221 agaaatggcc tgagcctatc aattgaatca tctagtatct gggtttaggc tacaaaactt
     2281 tatcagcaat taaggcaacg actttaggca atacttgata aagccagata ttatatatag
     2341 aaataataat taatcctacc tattctctat catgtggaaa aataaatatc aataaaaatt
     2401 aaattgttgc tctagttagg atgaattagc cagctgctgt aattctcttt tttcctgctc
     2461 agttagcaaa ttaagtggtt tgttttgcaa caccgtcatt ctctccttac gttgcatatc
     2521 cttaattcta gataataagc ctaaaaattc tgcttccaaa tcgatttcag tttcccatcc
     2581 taatttgagt tctgttgtct tattgagttc ttcagcatgc atcgtttcag aaaaatacaa
     2641 tagcgtttca gtaattttct gagtttctaa ttcctgtgac gataacgatt tataaaaact
     2701 cactaacgct tccaatgcaa gcacagtttg ctgaggtgca gcgttaagaa gacaagataa
     2761 atcacgatct aatttgttta caaaacgtgg atcatcgagc aaaactttga ttaatttatg
     2821 ataaggtgag ataggttgca atctcaaact ttcaatttgc ttacttgact gagaattagc
     2881 atttttaagc ttactttcaa aaatcttggt taacgcttct tgattcactc cagttaactg
     2941 agaaagccgt tttattaata ttaatttcaa tattggtgca gaaactaatt ggatcaatgg
     3001 ctttgcgatt tgaatcaatg cggcccgacc ctcgctcgat tcaagattaa ctcgcgaaga
     3061 caattctccg atcaataaat cagatagagg aattgctttc tcaaagagcg attcaaatga
     3121 ttgcttacca aatttacgaa taaaactatc aggatcttcg ccatcgggta aaaaaagaaa
     3181 acttatcttt ttaccgtcct tcaagtgagc gagactcgtt tccaatgccc gccaagctgc
     3241 cttcttaccc gcattatcac catcaaagct aaatataatt tcatcagttt ggcgcaaaag
     3301 cttttgaata tgtgacgagg tggtcgctgt ccctaaggtt gctacaacat attcgatgcc
     3361 atattgcgac agcacaacga catccatata cccttcaaca accaggacac gccttgattc
     3421 tcgaatcgct ttgcgagcta aaaataaatt atacaattcc cgatttttaa tgaacaacga
     3481 tgtttcaggc gaattcaaat attttggccc acccttctca tcaatcactc gtccaccaaa
     3541 gccaatgatc tgcccttttt gattaagaat tggaaaaata atacgcgctc ggaaccgatc
     3601 ataatatcta ccattattac ttctaataat caggccattt attgttaaaa aatggcctga
     3661 ttattacttc taataatcag gccatttttt aacaataaat tttctgattc atctcgatca
     3721 tagtcagtaa aaatttgtga gagactcctt agctcatcgg gcgcataacc aatcttgaag
     3781 cgagcagcaa tttctcccga taatccacgt tgttttaaat atgatatggc cttagttgca
     3841 tgtttcaatt gttcccgata ataatcagct gctcgctgca gctgatcagt catgcgttgc
     3901 ataacttgat tatcctgttt tttctcaata ggctcctggt tatcgatctc agtatcaact
     3961 tttaatcttg aacttgatcg attagctgct gcagtaataa ccgttttatc aggcattggc
     4021 ataccagcct gaacagctaa attttcaact gcatcaacaa agctaattcc gctaaattca
     4081 attacaaaat taatagcgtt tccgtgctta ccacacccaa aacaatgata aaactgtttc
     4141 gattgattta ctgagaatga gggagttttt tcattatgga aagggcagca tgcaacataa
     4201 ttgctacccg atttttttaa tggaatactt tgctcgataa tatcaacgat atcaatgcga
     4261 ttaagtaatt cctgaataaa cgattgtgaa atcatttatt aaatagggaa ggtagttaag
     4321 acttaaaaca acccaatgac tttccttaat taaatcctca agaatctacc tacgaatact
     4381 cgattaagtc agtgctttta ttttctcttt aattaatcca gatacataac ccatatctgc
     4441 gcgccccgct aattttggct ttaatgccgc cataacctta cccatgcttt gctgatctaa
     4501 ttgaccgacc gaggcaatta tttcatttat gatttgaata atctcctctt cactcaaagg
     4561 ttgtggcata taggtttgca aaacttctat ttcgaatttt tctgcttcgg ccaaatcttg
     4621 tcttttagcc atttcatact gtgtaataga atcttttcgc tgcttcagca ttttttcgat
     4681 gactgctgta atcgctacat tatccagtgt aattctttca tcgacttctc gctgcttgat
     4741 ggcggcttgt aacaatcgaa ttgcatcacg tcgcttagaa tcacctgaac gcattgctgc
     4801 tttcatatca tctgcaattt tttgtttcaa gtccatgatc acgatggtca ataaatcgag
     4861 actctactag taaagcttag gaggtaacag ctgactacgt aaccgtttat aagtccgctt
     4921 caccgcggct gcaagtttac gtttacgctc agcagtaggt ttttcgtaaa actcacgagc
     4981 tcgtaactcg gtcagtaaac ctgttttttc tactgcgcgt ttaaaacgac gcattgcaac
     5041 ttcaaatggc tcattttcct taactttaac tgtagtcata aaatctttat tacccctttc
     5101 tcactagaga attctaaaaa cccgcaatta taacagtgtt ttaagtagaa ttacccaagt
     5161 tttttctatt aattaatttt ttatttcccc aatcctaatc aattaaagga caggatcacc
     5221 caacaacatt attactcgct ctttatatta ctcttctcag gaacaagaaa aggctcaata
     5281 tccaatttca ctttataaat attggtctta ttcactttat cacgcttttt atcaataagt
     5341 accctgcaca tcgttacttg attggtcaaa ccctgctgac ttagccgctg ataaaaaatc
     5401 agtgcttctt ctgggcttaa atatccgata gttcgatcat ggatatcgat tctaacagcg
     5461 ttcttatcat ataaatgatc gctatcagga attaaatgtg catttaaaat cagtggtacg
     5521 tcaatagata ctgcggcagg gggcgatata ttctccttta gtagttgatt aatcgcacct
     5581 tgataagaat tacttttaac ctcatattga aactgattca attctggcca tgtataataa
     5641 ttttctaaat gatggtttga ttgtaaatcg ctattgctca gctcatcact cacagcatta
     5701 ctccttgcag caattttgtc gcgtgacgca attaaaacaa cagctgtaat cagtgtaata
     5761 ggaagaatat gatcattacc agcacctgcc cctgcaagaa ggccaacaca caatgcaaaa
     5821 ataataatag ataaaatacc caacttctca gacttcaatt tttgtacctg ccaaaaatgg
     5881 tataaattaa ttcagaatta acctcattta attacataac gtcatggatg aagaatcaag
     5941 cttaattgcg ctattcacaa gtagtttctt agctgcaacc cttctacccg gggggtcaga
     6001 agctgtattg atcggtgtgc taatggctta tcccgagtta tttacctcag cattaagtgt
     6061 tgcaacaatc ggcaataccc taggtggaat gagctcttac ctgatcggtc gatttcttcc
     6121 ggatgaaaaa gctattttga agaaaactaa taacagcctg cgaagcctcg agtggattca
     6181 tcgtcatggc tcccccatat tactgctgtc gtggttaccc attattggag atgcactttg
     6241 tgttgcagca ggttggcttc gcattcactg gttttatgct atcttattca ttgcagttgg
     6301 aaaattagcc cgctattggg tcatcgccac cgcaatcagc tagcgcacaa ctaagtacgc
     6361 tcaggaaaaa gttacttctc tagtaatttc ttgagttcac cactttggta catttccgtc
     6421 atgatatccg aacctccgac aaattcacca ttgacatata gttgaggaat cgttggccag
     6481 ttagagaaat ctttaattcc ttgccgaatt tcagggtcac tcaaaacatc tactgcaaca
     6541 aagtttgtta caccacaggc attcaatatc tttactgcat tagcagaaaa accacactgt
     6601 ggttggcttg gagtaccttt catatataaa acaactgggt ttgaactgat tagttgctca
     6661 attgattcac tggtagtcac actcaaaatc ctcacaaatt aataaaatta attaataaaa
     6721 atcagctact gctcatataa aaagttagtc atcaggggtc taagcaaata ttgatcactt
     6781 gcaaatggat aaccatcttt actgattcac acgtaatcca atacaatcag attcgctgcc
     6841 cacctgttat gcgtagaata cctgctaaat cagggtatga aaagatatta ataaaacttg
     6901 cttcttgtgc aaggagctgt ctacattgag cagcctggtt gtaaccatgt tccaatagta
     6961 actgtccacc agcgcacaga aattgagggg cttgcgcaat aatggtgcga atacatgcaa
     7021 taccatcttc aggcgctgac aacgcaatct ggggctcgaa acgtaaatcc ccttgatcaa
     7081 ggtgtggatc cccaatcgct acataaggtg gattagaaac aattaagtca tattgtccaa
     7141 caataccttg aaaccaatcc cccaaggtaa aatgtacatt gtcgacctca agcaatgttg
     7201 cattcatttg ggcgacctct agtgcttcag cagataagtc aatcgccata acttgcgcca
     7261 ggggtctatg cttagcaagg gtaagcgcaa tagctccact ccctgtcccc aaatcaagca
     7321 cagtatagct acgatccatt gggatgctag ctaaagcaag ctcaaccaat gtttcagttt
     7381 caggccgtgg aatgagaact gcaggtgtaa catgaaaagt caaatcataa aactcacgct
     7441 ggcctatgat ataggctatt ggctcaccat tgattcttcg ttgcagcaat gagtaaaagc
     7501 acatcatctt ctcagcatta acgaactcat ctgagtaggc tgctaaaaaa acaggggata
     7561 cttgcagtgc atgttgcagc aacaatcgcg catcgacatc gcttacatga tatttagcat
     7621 cgttaagcag ctgggcaatc gttactggca tttgcatcca gcctatttca gttgtaatcg
     7681 atcaataaca ctggaattgc aagatgaatg gacttcaaga acttcacaat taccagagat
     7741 attattaacg ctccatcaaa gactctaatt gagcagcctg atgttcggcc atcaatgctg
     7801 agcacaattc cccgatatct ccatccataa tttgatccat tttatagaga gtaagattaa
     7861 tacggtgatc tgttacccgg ccttggggaa aattataagt acgaatacgc tctgaacgtt
     7921 ccccagttcc taccaatgat tttcgagtcg ctgcctgttc tgcttgctgt tccctggttt
     7981 gcttatcaag aattcttgct gcaagtacac tcatggcctg cgctttattt ttatgctgcg
     8041 atcgaccttc ttggcactca acaacaatac cggttggcaa atgagtaata cggacagccg
     8101 aatcagtctt gttaatatgt tgcccacctg caccagatgc acggaatgta tcgattctta
     8161 attcggcagg gttgagttct acactggcaa tttcatctgc ttctggaatc acagccactg
     8221 tacaagtaga agtatgcacc cgaccttgcg tttctgttac aggaacacgt tgcactcggt
     8281 gcccccctga ttcaaatttt agccgtgagt aagcgccata cccgatgatc cgagcaataa
     8341 tctccttata accaccgaca tccgatgggt tctgagaaat gatttcaact tgccatccct
     8401 gccgctctgc aaaccgtgca tacatcctga acaaattacc agcaaacaac gcagattcat
     8461 ctccccctgt tcctgctcga atttcaagaa agatattgcg ctcatcactg ggatctttag
     8521 ggagaagctg tttaagtaac tccgtttcaa tctgttgtaa cttctccttt ccagtaataa
     8581 gctccgcttc tgcaaaatca cgtaaatgag ggtctgcact catttccttc gcggtttgaa
     8641 tatccagctc agtttgttgg tgcgcatgat ataactcaac gacaggaata atctctgaac
     8701 gttctcgggt aattttccgg taattatcta aatccgttgt taccttctca ctactcaaaa
     8761 gttgatttag ttcttcgagt ctcacactta accgtgtgag acgttctgca atacttttgt
     8821 tcattgcg
//
LOCUS       DAOQKQ010000111         8496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_4004_length_8496_cov_4.407890, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000111 DAOQKQ010000000
VERSION     DAOQKQ010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 8496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8496
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4004_length_8496_cov_4.407890"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="PKZ49_08275"
     CDS             <1..844
                     /locus_tag="PKZ49_08275"
                     /inference="COORDINATES: protein motif:HMM:NF024661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4055 domain-containing protein"
                     /protein_id="HND36595.1"
                     /translation="SKNDKGVETHRVLKLDDSGLYMQELYEIKNGKETLIDTIIPMIG
                     GKRFDYIPFTIIGVDSLDWDVEIPPLIDLVNVNMHHYVMSSAYERGCFLSGLPSLFIY
                     GNRDDDKVIYIGSSMANSFPDPQTKAEILEVRGNFGALKENLLSKEMQMAALGAKILE
                     PQKAGVESEGALARRMSGEESVLADMAGTISNGMTKVFYIYLSWLGINDESFTYEINR
                     EFLPSSLTSQEISALFLAWQGGAISYETLFYNYKRASLIPENVDFEEEQDRALNGRNI
                     PNPV"
     gene            940..>1688
                     /locus_tag="PKZ49_08280"
     CDS             940..>1688
                     /locus_tag="PKZ49_08280"
                     /inference="COORDINATES: protein motif:HMM:NF016148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HND36596.1"
                     /translation="MAKLEKQVIKTLDANIISEKYPDLPDFTEFTQHSIDLAMQDFSK
                     APSLAVIDRLYKPRFEGNVMRDWWTRAGEDLKFKVKGVIRQGTIEGWDKATMTSFLRS
                     SFDGNRRQAAAVVQTTIHTISNEARMAVYEANDDIIGGFYWLSTLDSRTTIPCVGRSG
                     LQWDINKEPMGHNIPFATPPIHWNCRSIMMPKLLTFKDVGVNLGEANITRSSKLGQID
                     SKISFDKFLGMLSKSEQDDQLGIGRAQLWRDG"
     assembly_gap    1689..1788
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1915..2613
                     /locus_tag="PKZ49_08285"
     CDS             1915..2613
                     /locus_tag="PKZ49_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36597.1"
                     /translation="MIKTVVDKVEDIPEALKEFYEEREGKFYLQTDESKALKSALERE
                     KEGSRKLKEMLAEYQSKFEEAEKKKEKEKLDDGDIEAVIESRLAKYKQDFESKLNAEI
                     ATKNSLMSSALKAEIRSNALKNGVNSDAVEDAILRGSGLFVIDADGSIVAKKDGQVVF
                     GRDGKTPYSISEWLEEIKPNTSHWYADQNKGGAAPGVKNMNSKSKTMQRAQFDKMSSY
                     EKMTFAKEGGKVTD"
     gene            2641..3786
                     /locus_tag="PKZ49_08290"
     CDS             2641..3786
                     /locus_tag="PKZ49_08290"
                     /inference="COORDINATES: protein motif:HMM:NF023081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HND36598.1"
                     /translation="MPNVLNNLAADLYRAADMVGRELVGFIPSSTLNTAEGQRVAKGG
                     IVRSHFTRTPTVGSTYAPSMTVPEGTDQTVDNKTLTVNQYASVQIPWTGEDIKHVNNG
                     AGYETIYGDQLAQAMRAISNTIESYVAGVVKAGSSRAFGTAGTTPFASNFNEVAELRQ
                     ILVDNGCPMDNQVTLVINSLAGTKLRNLAQLQKVNESGGSELLRQGTLLDLQQIMIKE
                     SAGISLHTKGTGSGYLVNSASITAGTTTIPADTGTGTVLAGDVVTFAADTTNKYVVNT
                     ALAGGSLALGAPGTRVSIADNNAITVGNNYTPNVAFHKSAVEVVCRAPELPSAEIAID
                     VMDVTDPWSGLTFQVAVYAGYQKSMIEVRCFYDAVVWKPNHVATLLG"
     gene            3794..4306
                     /locus_tag="PKZ49_08295"
     CDS             3794..4306
                     /locus_tag="PKZ49_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36599.1"
                     /translation="MALIVEDGSMVAGAESYASITYANNYFILLGNEAWGDLDLSTKE
                     QALRKATQFMQNEYFSMWQGVKRQYTQALDWPRENVYIAGTDSLVANSIIPNEIKNAC
                     CLLAYKASVGALYSDKERSIKREKIDVLETEYFESDSNQKEYREIDAMLRRYMISASA
                     KSGALPIVRV"
     gene            4344..4661
                     /locus_tag="PKZ49_08300"
     CDS             4344..4661
                     /locus_tag="PKZ49_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36600.1"
                     /translation="MAVTINYKTPGAYDVNTGSISNTTLSQTGYAVLVNNAFGDDRKS
                     SSSEVSEHDVLITILLSSKGINTPYLNDEIVISGVSHTITRIKTISPNYDEAIIYIVE
                     LKR"
     gene            4658..5089
                     /locus_tag="PKZ49_08305"
     CDS             4658..5089
                     /locus_tag="PKZ49_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008060694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36601.1"
                     /translation="MSFKGDVSRFTEKTNARSSKFVRLLVSKLGESLIMKTPVGDPSG
                     WKTKHPPAGYVGGTARANWQYGNGFIPNEFLNEQDGDGNRTLSRIINKTSSGDPYTVH
                     YITNNVPYIQILEDGHSSQAPQGMLKVTLIEFKSIFNAIKV"
     gene            5086..5484
                     /locus_tag="PKZ49_08310"
     CDS             5086..5484
                     /locus_tag="PKZ49_08310"
                     /inference="COORDINATES: protein motif:HMM:NF024943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator-like protein"
                     /protein_id="HND36602.1"
                     /translation="MTVSKIQIALENSINNIVPALPTKWPNTRPIIALPYQDVYFFFA
                     PPVHEEYSTGLHRIDGILQINLFYKLYTGKSEAMARAELIRLAFARGTSHSHDNVIVN
                     ISKSPRIKQSVEENDRFMIPIDIEFYSYIE"
     gene            5496..6704
                     /locus_tag="PKZ49_08315"
     CDS             5496..6704
                     /locus_tag="PKZ49_08315"
                     /inference="COORDINATES: protein motif:HMM:NF039819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HND36603.1"
                     /translation="MAIGSGVDKQVIYKKQTALGTKATASGAQVLRRTTSGIELKKNT
                     FQSNEIRTDYQVADFRHGSKYIEGPISGEISPATYKDFMAAAVRKDFVAGSSIASLTL
                     TTAGSGPTYTITRSAGSWITDGVKVGDIVRVTAGLAAGNLNKNYLVASLTATVLTVYV
                     LNGTSISAQSAIAACTLSVTGKKTLVPQSSHTADYFTIEQWYGTGVAMSEQFYDCKVN
                     SMDIQLPASGMATCSFGFLGRNLDIGSAAYFTSPTAATTTGVMAGAQGILIVGGASVG
                     NVTGMNFTLNGSMTKEDVVGASSTPDVFSGSVLISGQMTILLENDTYMQMFEDETEAS
                     VTMALTATSAANSDFVAFTMPRIKVGGNSKDDGQKGIVQTVPFTALLNTNSATNNGDV
                     TTLMIQDSAA"
     gene            6730..7143
                     /locus_tag="PKZ49_08320"
     CDS             6730..7143
                     /locus_tag="PKZ49_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36604.1"
                     /translation="MSGFGKLNITNICDMPTEFEYMGEDGVTKTGFYISVLGQYSKKV
                     QSFILELENKDRMRLFEERKRGKQEGFKKAEQDMDDTIEAIAVRVCGWRGTVNGVEVP
                     EYSEENAIELMKINPIIRSKVLEISNDLANFTKSK"
     gene            7209..7472
                     /locus_tag="PKZ49_08325"
     CDS             7209..7472
                     /locus_tag="PKZ49_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36605.1"
                     /translation="MRDHLLAAYRQTGRKHDMLIELPIGIFSNIWAWFCELDSARQVS
                     ELGISPLSYQEIDSWARLTDKSLSSHDVSIIKDLDRLRLRMIK"
     gene            7473..>8496
                     /locus_tag="PKZ49_08330"
     CDS             7473..>8496
                     /locus_tag="PKZ49_08330"
                     /inference="COORDINATES: protein motif:HMM:NF040129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HND36606.1"
                     /translation="MTDFAALTLTLDSTSIKGGISELNRLGDAGDKAERNISRSTGII
                     KDSFDSIKNAAIGVVSAYGALSTIKGLANAAIDVEKFESALKVATGSAKAANAELDFI
                     RKTSNELGLSLRDTTDQYSKLAVAAKGTKLEGQAVREIFVGISQAATALKLRSDETSG
                     ALLAIQQIISKGTVSAEELRGQLGERLPGAFQIAARSIGVTTEELGKLLQKGELTAEV
                     LLPNFARELQRTFGAESQAAAAGLQSQINRFNNALFELQVAIGRVGIIDFFTSATQSV
                     SVFLSKSADIVKLYGNDVRIAFAAVIDVGRGVINTFHAAGLGIASIVAQVKALLQLDI
                     KKAISID"
BASE COUNT         2696 a         1473 c         1990 g         2237 t
ORIGIN      
        1 gagtaaaaat gacaagggag tagaaacaca tcgcgttttg aaacttgacg actctggcct
       61 gtacatgcaa gaactttatg agattaaaaa cggaaaagaa acgcttatag acacaatcat
      121 accaatgata ggcggtaaaa gatttgacta cataccgttt actataattg gagtagattc
      181 tctggactgg gatgttgaga tcccgccact aatcgatttg gtaaacgtaa acatgcatca
      241 ctatgtaatg tcatcggcat atgagagagg atgcttttta tctggcttgc cttccttgtt
      301 catatacggc aatagagatg atgacaaggt gatttacata gggtcatcta tggcgaatag
      361 ttttccagat cctcaaacta aagcggaaat acttgaggta agaggaaact ttggtgcgct
      421 aaaagaaaat ctactgtcta aagaaatgca aatggctgca ctaggggcta agatattgga
      481 gccgcaaaag gctggtgtag aatctgaggg tgcgctagct agaaggatga gtggtgaaga
      541 atctgtcctg gcagacatgg caggaactat atctaacgga atgacaaaag tattctatat
      601 ttatctgtca tggcttggga ttaatgatga atcgtttact tatgaaataa atagggagtt
      661 tttgccttcc agcctgacat cgcaagagat aagcgcttta ttcttggctt ggcaaggcgg
      721 cgctatatcc tacgaaacac ttttctataa ctacaagcgc gcgtcattga tacctgaaaa
      781 tgtggacttt gaggaagagc aggatagagc gctaaacggt aggaatatac caaatccagt
      841 ttaatgataa atcaatcgca gctattaatt aagcttttaa agtaccagct ttctctgcgt
      901 aggtatgagg ccggtcaaag gcgagcgatt gaacaggttt tggcaaaact agaaaagcaa
      961 gtaataaaaa cgcttgatgc taatatcata agtgaaaaat atcctgattt acctgatttt
     1021 acagaattca ctcagcacag catcgatctt gccatgcaag attttagcaa ggccccatca
     1081 ttagctgtaa tagataggct ttacaagcca aggtttgagg gcaatgtcat gcgtgactgg
     1141 tggacaaggg ccggtgagga tttaaagttc aaggttaaag gagttattcg tcaaggaact
     1201 atagagggat gggataaggc aactatgacg tcgttcttgc gttcgtcatt tgatggtaac
     1261 aggcgtcagg ctgctgcagt agtgcaaact accattcata cgatatcaaa tgaagcaaga
     1321 atggctgtgt atgaagccaa tgacgatatc ataggcggtt tttattggct atcaacgctt
     1381 gacagcagaa cgacaatccc ttgcgttggt aggtctggtt tgcaatggga cattaacaag
     1441 gaaccaatgg gacataatat tccatttgca acccctccga tccattggaa ttgtcgatca
     1501 ataatgatgc ctaagctatt gacatttaaa gatgttgggg ttaatcttgg agaggcgaat
     1561 attacaagat catcaaaatt aggacaaata gatagtaaaa ttagcttcga taagtttctt
     1621 ggcatgctat ccaaatctga acaggatgat caattaggca ttggtagggc gcaattatgg
     1681 cgcgatggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa tctgaacagg
     1801 atgatcaatt aggcattggt agggcgcaat tatggcgcga tggaaagata acattatctc
     1861 agttgctaga tgctactggg cgtgaactga ctctagggga gctaagagcg cgatatgatt
     1921 aaaaccgtgg tagataaagt agaagatatt ccagaagcat taaaagaatt ttatgaggag
     1981 cgagaaggta agttctatct tcagacagat gaatcgaagg cactgaaaag cgctttagag
     2041 agagaaaaag aaggctctag aaaattaaag gaaatgcttg cggaatatca atctaagttt
     2101 gaagaagcgg aaaagaaaaa agaaaaggaa aagcttgatg acggcgacat agaagcagtt
     2161 atagaaagcc gtctggctaa gtataagcaa gattttgaat caaaattgaa tgcagagatt
     2221 gcaacaaaaa atagcttgat gagcagtgct ttaaaagctg aaattagaag caacgcattg
     2281 aaaaacggcg taaattctga cgctgtggaa gatgctatat tgcgtgggtc tggtttgttt
     2341 gtaatagatg ctgatggtag catagtcgct aaaaaagatg gtcaggttgt ttttggaaga
     2401 gatggaaaaa ctccatactc gatttccgaa tggctagagg aaattaaacc aaatacatcg
     2461 cactggtatg ctgaccaaaa taaaggcggc gctgctccag gtgttaagaa tatgaactcc
     2521 aagtctaaaa caatgcagag agcgcaattc gacaaaatgt catcgtatga aaaaatgaca
     2581 tttgcaaaag aaggcggaaa agttactgat taaactattt ttttaaaagg atattgaatt
     2641 atgccaaatg tattgaataa tttagctgca gatttatatc gtgcagcaga tatggtagga
     2701 cgtgagctgg ttggttttat tccatcgtca actttaaaca cagcagaagg ccagcgtgta
     2761 gcgaaaggcg gtattgttag atcgcacttt acacgcactc ctactgtcgg tagtacttat
     2821 gctcctagca tgactgtacc ggaaggcaca gatcaaacag ttgataataa gactttaact
     2881 gtcaatcagt atgcgtctgt tcaaattcca tggacaggtg aagatattaa acatgtaaac
     2941 aatggcgctg gatatgaaac aatttacggc gatcaattag cgcaagcaat gcgggctatt
     3001 agtaatacta tcgagtcata tgtagccggt gtagtaaaag caggctcatc tcgcgcgttt
     3061 ggtactgctg gcactactcc attcgcatca aactttaacg aggttgctga gctgcgtcaa
     3121 atccttgttg ataatggatg cccaatggat aatcaagtga cattggtgat taattcgttg
     3181 gctggtacta aattacgtaa tcttgcgcag ttgcaaaaag tgaatgaatc aggtggcagc
     3241 gagttgttga gacaagggac attactagat ttgcagcaaa ttatgatcaa agagtcggct
     3301 ggtatttcct tgcatacaaa aggtaccggc tctggctacc tagtaaacag cgcttccatt
     3361 accgcaggaa cgactaccat acctgctgac accggcaccg gtactgtttt agctggcgat
     3421 gtggtaacct ttgctgctga cacgacaaat aaatatgtcg tgaatacagc acttgcaggt
     3481 ggttctttag cgcttggcgc cccaggtact cgcgtttcta ttgccgataa taacgcaatt
     3541 actgttggca ataattacac gcctaatgta gccttccaca aatcggctgt tgaggttgtt
     3601 tgtcgtgctc ccgagttgcc atcggctgag attgcgattg atgtgatgga cgttacagac
     3661 ccgtggtcag ggcttacttt ccaagttgcg gtatatgctg gttatcaaaa atcaatgatt
     3721 gaagtacgtt gcttttatga tgctgtcgta tggaaaccta accatgtagc tacattgcta
     3781 ggataatatc cagatggcat tgattgttga agatgggtca atggtggcgg gcgctgaatc
     3841 ttatgcgtcc atcacatacg ctaataatta tttcattctg cttggtaacg aagcctgggg
     3901 tgatttggat ttatcaacta aagagcaggc gcttcgcaag gcaacgcagt ttatgcaaaa
     3961 cgaatatttt agcatgtggc agggcgtgaa aaggcaatac acgcaggctt tagactggcc
     4021 acgtgaaaat gtttatattg ctggcactga ctcattagtt gctaatagca taatacctaa
     4081 tgagattaaa aacgcatgct gtctgcttgc gtataaagca agcgttgggg ctttatattc
     4141 agataaagag cggtccatta aacgtgaaaa gattgacgtt ttagaaactg aatactttga
     4201 gtcagatagc aatcaaaaag aatacagaga aatagatgca atgctgcgta gatatatgat
     4261 atcagcatcg gcaaaaagtg gagcgttgcc aattgtacga gtctaatatt acatcggcat
     4321 acaaccttat atcgtctaag ggcttggctg taactataaa ttacaaaacg ccaggtgctt
     4381 atgatgttaa taccggctca attagcaata caacgctatc gcaaactggt tatgctgtat
     4441 tggtgaataa tgcttttgga gatgatagaa aatcaagctc tagtgaagtc tctgagcatg
     4501 atgtattaat aactattctt ttgtcatcta aaggcataaa cacaccatat ttgaatgatg
     4561 aaattgtcat aagtggagta agccatacaa taacaagaat aaaaacgata tctccgaatt
     4621 atgatgaggc aataatatac atagtggagc tcaagcgatg agctttaagg gtgacgtgtc
     4681 aaggtttaca gaaaaaacaa acgcaaggtc aagcaagttt gttaggttgc ttgtaagtaa
     4741 gcttggcgaa tcattgataa tgaaaacgcc agtgggtgac ccatcaggat ggaaaacaaa
     4801 acaccctcca gccggttatg ttggcggaac tgcaagagcg aattggcaat atggcaacgg
     4861 gtttattcca aatgaatttt taaatgagca agacggtgac ggaaacagaa cgctgtcaag
     4921 aataataaat aaaacaagct ctggcgatcc ttacacggtg cattacataa ccaataatgt
     4981 cccatacata cagatcttag aagatgggca ttctagtcag gcgccacaag gaatgctaaa
     5041 agtaacactg attgagttta aaagcatttt taatgctata aaagtatgac ggtatcaaaa
     5101 atacaaatag cattagaaaa cagcattaat aatattgtac cggcattgcc tactaaatgg
     5161 ccaaatacaa ggccaattat tgcattgcca tatcaagatg tttatttttt ctttgctcca
     5221 ccggttcatg aggaatactc aaccggattg catagaatag atgggatttt gcagattaat
     5281 ttgttttaca aattgtatac gggaaaatct gaagctatgg caagagccga gcttatacga
     5341 ttggcatttg cgagaggaac tagccattct cacgacaatg taatcgtaaa catcagtaaa
     5401 tcacctcgta taaagcaaag cgttgaggaa aatgacaggt tcatgatccc aattgatatt
     5461 gaattttaca gttatattga ataggagatt gaatcatggc aataggttca ggtgtagata
     5521 aacaagtaat ttataaaaag caaacggctt tgggcaccaa agccacggct tcaggcgctc
     5581 aagtgctaag gagaaccaca agcggcattg agctcaagaa aaatacattt cagtctaatg
     5641 aaattcgcac agactaccaa gtggctgatt ttagacacgg gtcaaaatat attgaaggcc
     5701 ctatcagcgg tgaaattagt ccggcaacat acaaggattt catggcggct gctgtgagaa
     5761 aggattttgt agctgggtct agtatagcgt cattaacact gacaacagct ggttccggcc
     5821 ctacatacac aatcacaagg tcggctggat catggatcac agacggtgtt aaagtcgggg
     5881 atattgtgcg cgttacagct ggtctggctg caggaaattt gaataaaaat taccttgtgg
     5941 cttctttgac tgcaacagta ctaactgttt acgtgctgaa tggtacttca atatcggctc
     6001 aatctgctat tgctgcgtgt acgctatcag taacaggtaa aaaaacatta gttccgcaat
     6061 cgtcacatac cgcggattac ttcaccatcg aacaatggta cggtactggg gtggcaatga
     6121 gtgagcaatt ttatgattgc aaagttaact ctatggatat tcaattgcca gctagtggaa
     6181 tggctacttg tagcttcggt tttctcggta gaaatttaga catcggttcg gctgcttact
     6241 tcacttcccc aacagccgcc accactacgg gtgttatggc gggcgcgcag ggaatattga
     6301 ttgtaggcgg tgcgtctgta ggtaatgtta caggaatgaa ttttacgctt aacggtagca
     6361 tgacaaaaga ggatgtggtc ggggcgagtt caacacctga tgtatttagt ggatcagtgt
     6421 taatttcagg acaaatgact atcctgttag aaaatgacac ttacatgcaa atgtttgagg
     6481 acgaaacaga ggcatctgta acaatggcat taactgcaac aagcgcggct aattctgatt
     6541 ttgtagcgtt taccatgcct cgcatcaaag taggtggaaa cagtaaagat gatgggcaga
     6601 aaggtattgt acaaacagtt cctttcacag cgcttctcaa taccaactcc gctacaaaca
     6661 atggcgatgt aactacgcta atgattcaag attcagcggc gtaattttta ttaattaaag
     6721 gagatataga tgtctggatt tggaaaattg aatattacta atatttgcga tatgccaact
     6781 gaatttgaat atatgggtga ggatggcgta acgaaaacag gcttttatat ctccgtactt
     6841 gggcaataca gcaagaaagt tcaatccttt attttagagc ttgaaaacaa ggatagaatg
     6901 cgcttgtttg aggagcgcaa acgcggtaaa caggagggat tcaagaaggc agagcaagac
     6961 atggatgaca ctatagaggc tatagcggtg cgtgtatgcg gttggcgtgg aactgtaaac
     7021 ggtgtagagg tgccggaata tagtgaggaa aatgctattg agctgatgaa gattaaccca
     7081 attattcgtt caaaggtgct tgaaataagc aatgatcttg caaattttac aaagagcaag
     7141 tagatattct gcttgcatat gccgaaaggc aatttaattt agaaaagcga caagaagatg
     7201 gtgcaacttt gcgtgatcac cttcttgcag catatagaca aactggaaga aaacatgata
     7261 tgctgattga attaccaatc ggcatatttt caaatatttg ggcttggttt tgtgagcttg
     7321 actcagcgag gcaggtcagc gaattaggca tcagtccatt atcatatcaa gagatagata
     7381 gctgggcaag gttgactgat aaaagtttat ctagtcatga cgtaagcata attaaggatt
     7441 tggatagatt acggcttagg atgattaaat aaatgactga ctttgcagcg ctaacgctaa
     7501 cactagattc aaccagcata aagggcggaa tatcagagct taaccgctta ggcgacgctg
     7561 gtgataaggc cgagcgcaat atatcaaggt caacaggaat aattaaagat tcttttgact
     7621 caataaaaaa cgctgcaata ggcgtcgtaa gcgcttatgg agcgctatca actatcaagg
     7681 gacttgctaa cgcggccatc gatgtagaaa agtttgaatc cgcattaaaa gtggcaacgg
     7741 gaagcgctaa ggctgcaaac gcagaacttg attttataag aaaaacatca aacgagctcg
     7801 gtttatctct tagagatacc acagatcagt attctaagct tgcggtagct gctaagggta
     7861 caaagcttga aggtcaggct gttagagaaa tattcgttgg aatatctcaa gctgcaacag
     7921 ctttaaaact aaggtcagat gagacatctg gcgccctgct tgcaatacag caaataatat
     7981 caaagggcac tgtatcggct gaagaattaa gaggccagct gggggaacga ttgccaggtg
     8041 cttttcagat tgcagccagg tcaataggcg tgacaactga agagttgggc aagctgctgc
     8101 aaaaaggcga attaacagca gaggtattgc ttcccaattt cgcgagggaa ttacaaagaa
     8161 cattcggcgc agagtctcaa gctgcagcag ctggtttgca atcccaaata aatagattta
     8221 ataatgcgct ttttgagttg caagtagcaa ttggaagggt cggtataata gatttcttca
     8281 cgtcagcgac tcaatctgtt tcggtgtttt tatcaaaaag cgccgatatt gttaaactat
     8341 acggaaatga cgtgcgcata gcgttcgcag cggtaattga tgttggtaga ggcgtgatta
     8401 atacgttcca cgctgctggg ctaggaatag catctatagt ggctcaggta aaagctttgt
     8461 tgcagcttga tattaagaaa gccatatcaa tcgatc
//
LOCUS       DAOQKQ010000112         8484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_4018_length_8484_cov_5.633053, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000112 DAOQKQ010000000
VERSION     DAOQKQ010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 8484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8484
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4018_length_8484_cov_5.633053"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1777
                     /locus_tag="PKZ49_08335"
     CDS             <1..1777
                     /locus_tag="PKZ49_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HND36607.1"
                     /translation="DWAANIGLTQVLGKSSLIDANIGYTHSSGFMENPYKATTVIFID
                     PDTISPNPADSIRGNVRAVLEQRPDYRNQLAISNRFVQHISPLDAALHLNYKFSFDDW
                     GINAHTFESDWIQPLFEGWTLTPRIRYYSQSAADFYQPYLFSNQAFLKTVTNSAGETQ
                     AITYDPRKLPQDFASDHRLASFGSLSGGISLNKRFARGFELEAGFEYYTRASAFAFGG
                     GNSSFADFDYYVANAALKIGLETLNVNQIGRNRSAPGAAHDHTAQHDHAFIPAGIMFG
                     HTLDKAGDIMVGYRFMYARQTGDILRGSHVVSDLEIVNQACGDLVLCRFAPDYMNMRM
                     HMLDIMYAPTRWLNLMLMPQFMDMEMNLRPLEGRPPLAPGGHDHPGTLHATGGVADTI
                     MMPLIKLLDQPNHRLHIGLGFSAPTGSVDQEVRRMAKIDGGIIHFDMQLGSGTWDFMP
                     NLTYTGSHSQWYWGGQLYGVKRLENKNESGYRLGDLFQASTWGGYRLTNWLAASVRGV
                     YTTRGAIHGDFNTFNARIGPMDFPHNQGGQFWDLGIGINARVPSGRLVGNHFAFEWLQ
                     PLRDDFNGYQLERQGALSATWHYSF"
     gene            1783..2733
                     /locus_tag="PKZ49_08340"
     CDS             1783..2733
                     /locus_tag="PKZ49_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HND36608.1"
                     /translation="MKLYQYTFKAMGTPCEIQLYGAVESEVSQVAESAIADVRRLETA
                     YSRYRDDSFLSEINRVAAHGGSIQVDSETAGLLDYAEACYHASDGLFDVTSGILRQAW
                     NFKSNQLPDEQTIQSLLDRVGWEKVHWLSPNLEFPVAGMEIDLGGVVKEYATDRAASI
                     CLDRGIQNGMVNLGGDIKVIGPHIDGSPWKIGISHPRQPATLLHTLALHSGALASSGD
                     YERCITINGIRYGHVLNPKTGWPVRKMAAVSVVGDFCVVAGSASTIGMLKDEEAKDWL
                     AILGLPHLWVNVSGEVGGSLLDTVQVTKIYNHDEAQRYPY"
     gene            complement(2690..3424)
                     /gene="ubiE"
                     /locus_tag="PKZ49_08345"
     CDS             complement(2690..3424)
                     /gene="ubiE"
                     /locus_tag="PKZ49_08345"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HND36609.1"
                     /translation="MTNTTHFGFKTVEESEKAHKVADVFHSVAARYNLMNDLMSMGLH
                     RLWKRFAIDASGAKKGDRILDIAGGTADLSALFLEQVGESGQVWLTDINNSMLTIGRD
                     RLLNDGKAVPATQCDAEKLPFPDNYFDHVCVAFGLRNMTHKDIALKEVLRVLCPGGSV
                     IVLEFSKIWKPLQPLYDAYSFKALPFIGKFVAQDEESYRYLAESIRMHPSQEELKQLM
                     QDVGFERVEYFNLSTGIVALHRGYKF"
     gene            complement(3466..3837)
                     /locus_tag="PKZ49_08350"
     CDS             complement(3466..3837)
                     /locus_tag="PKZ49_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HND36610.1"
                     /translation="MAGLNKNTPTPTEIKFHKQSKLLEITFNNNQKFQLSSEFLRVYS
                     PSAEVQGHGPGQEVLQIDKQDVTIVKIEPIGQYAIQPHFSDGHNTGIYSWDLLYNYGV
                     NQEKMWHHYLQRLEAAGIKHD"
     gene            3952..4677
                     /gene="phoB"
                     /locus_tag="PKZ49_08355"
     CDS             3952..4677
                     /gene="phoB"
                     /locus_tag="PKZ49_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HND36611.1"
                     /translation="MAATILIVEDEPAIQELISYNLRNTGFIVACAGSAEQAKIMVND
                     VLPDLILLDWMLPGTNGIDFARYLRQGARTKAIPIIMLTARVDESDKVSGLEVGADDY
                     VIKPFSPRELVARIKAVLRRRLPEASDEIVETGGLKLDPATHRVMVTDSADKSKSIEV
                     NLGPTEFRLLHYLMTHTERVHTRGQLLDRVWGDHVFVEDRTVDVHIRRLRRALEDVGK
                     DDLIQTVRGAGYRFSYQCEVDTH"
     gene            4949..6325
                     /gene="phoR"
                     /locus_tag="PKZ49_08360"
     CDS             4949..6325
                     /gene="phoR"
                     /locus_tag="PKZ49_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HND36612.1"
                     /translation="MFEIGRRFVGIVLLIIVSVILGFFFDETVALLFLSSILLIIVSY
                     HIISLASLDRWLQRYDPSSQVMASVPNNFGAWGDVFVRLTRYMRRYYRSRQSLSRALE
                     RLQRATSAMPDGIVILDKIDRIEWCNPVAEQHLGLDLKMDIGQHITHLVRQMQFVEYL
                     TSGDFSKPVIIKQSRYQEIMLSLQLVPYGDEEKLLISRDITRFEKMEIMRRDFIANVS
                     HELRTPLTVINGFLEMLQDNGMKDSDMEKHALGLMSQQSRRMQRLVEDLLTLSRLENG
                     QNVLREEEVDMKELLQNLLHEAQSLSAARHTITLQLKKDDINVLGSADELRSALGNLV
                     SNAVRYTPDGGDIVLSWGEEKGLFYVRDSGIGIEPQHIPRLTERFYRIDRSRSRETGG
                     TGLGLAIVKHILNRHQARMDIISELGKGSTFKVWFPLKRLQATTKNSLPEVVEAQLEH
                     SQFMPPQQ"
     gene            complement(6397..6473)
                     /locus_tag="PKZ49_08365"
     tRNA            complement(6397..6473)
                     /locus_tag="PKZ49_08365"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6437..6439),aa:Val,seq:gac)
     gene            6534..7526
                     /locus_tag="PKZ49_08370"
     CDS             6534..7526
                     /locus_tag="PKZ49_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HND36613.1"
                     /translation="MKSTKYNPPRWLWGGNAQTIYPYLMSQPASIPYRRERWELDDGD
                     FIDVDWLDGEGNKPLVVLFHGLEGGSRSHYALSMMRYLQSCQWNGVVIHFRGCSGYPN
                     RLPRAYHAGDSAEIDWMLKRIASIQSSQPQKSNRTIYAMGVSLGGNALLKWLGEQGKI
                     ANQHIAAVAAISVPMDLVAAGNVLDKGFNQLYTRHFLSSLKQKIVEKQKKFPGLFDIK
                     AVTQCATLYEFDNLVTAPLHGFRDTDEYWTTSSSKPWLRAIEVPTLVLNAKNDPFLPA
                     HALPEYSAVSSAVTLEYPEQGGHVGFLHGPFPGKLMWLPERILRFFEGVNCNNQ"
     gene            7644..>8484
                     /locus_tag="PKZ49_08375"
     CDS             7644..>8484
                     /locus_tag="PKZ49_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectin acetylesterase-family hydrolase"
                     /protein_id="HND36614.1"
                     /translation="MLRFDRITLILLIFFFSEAYAVGWEKIEVSNTELTILDDNGQYK
                     DIKPGCAFSYLPDEAGLPNKPFHFYYRKGTKPQTLIFLNGGGACWNDATCTTSLTVPV
                     SSTTRPAYNPSMDFENDPESVGGILDFARADNPLKDWNMVFIPYCTGDAHIGSNDKVY
                     ADPLGIINAGNPIMVQHRGFDNFMAVREWLKHRPDRFNTEKVLVAGSSAGAYGALMNF
                     SRLHRIYPSMTKISLLSDAGAGVFTNNFLNTVFEPNGPWGTENTLATWIPGINQISSY
                     NDLN"
BASE COUNT         2314 a         1623 c         2095 g         2452 t
ORIGIN      
        1 agattgggcc gccaatatcg gattaactca agtgttggga aaaagttcac tgatcgatgc
       61 caatattggg tatacccata gcagtggttt tatggaaaat ccctataagg ccactaccgt
      121 tatctttatc gatcctgata ccattagccc aaaccccgct gactccattc gcggcaatgt
      181 gcgagccgtg ttagagcaac gacctgatta tagaaatcaa ttggcaatca gtaatcggtt
      241 tgtgcaacac atcagtcctt tggatgcagc gttacatttg aactataaat tttcattcga
      301 tgattggggg attaatgcgc atacctttga gagcgactgg attcagccgc tgtttgaggg
      361 ttggacgttg accccacgga tacgctatta ctcacaaagt gctgctgatt tttatcagcc
      421 ctatcttttt tcaaatcagg cattcttaaa aacggttacg aatagcgccg gtgaaactca
      481 agccattacc tacgatcccc ggaaactgcc ccaagacttt gcgagcgatc atcgcttggc
      541 aagttttggc tctttgagtg ggggaatatc cctgaataaa agatttgcac gtggtttcga
      601 gcttgaagcg gggtttgaat actatacacg tgccagtgcg tttgcatttg ggggagggaa
      661 tagtagtttt gccgattttg attactatgt tgctaatgca gcgcttaaaa ttggacttga
      721 aacactgaat gtgaatcaga tcggcagaaa ccgttctgcg ccaggtgcag cgcacgacca
      781 tacagcacaa cacgatcatg ccttcattcc cgcagggatc atgtttggtc atacactcga
      841 taaagcgggc gatatcatgg tcggttaccg tttcatgtat gcgcgtcaaa caggcgatat
      901 tttgcgtgga tcacatgttg ttagtgattt ggaaattgtc aatcaagctt gcggtgattt
      961 ggtcctttgc cgttttgcgc ctgactatat gaatatgcgg atgcatatgt tggatatcat
     1021 gtatgcgcca acccgctggt tgaatttgat gctgatgccc caattcatgg atatggagat
     1081 gaatcttcgc ccgctagaag gtcgaccacc gcttgcaccg ggagggcatg atcatcccgg
     1141 tacactgcac gcaaccggcg gggtggctga taccatcatg atgccactga tcaagcttct
     1201 cgatcagcct aaccaccgac ttcatatcgg gttggggttt agcgcaccta ccggaagtgt
     1261 tgatcaagaa gtcagacgta tggctaagat tgatggcggt atcatccatt tcgatatgca
     1321 gctcggaagt gggacttggg atttcatgcc gaatctgacc tataccggga gtcattccca
     1381 atggtattgg ggtggccagc tatatggtgt gaagcgttta gagaataaaa atgaatccgg
     1441 ttatcggctc ggtgatcttt tccaagcctc gacttggggt ggctatcgtc taacgaactg
     1501 gttagcggcg tccgtgcgcg gtgtttatac gacaaggggg gctattcatg gggatttcaa
     1561 tacattcaat gcacgcattg gcccaatgga ttttccacat aaccagggtg ggcagttttg
     1621 ggatttagga attgggatta atgcaagggt tccgagtgga cgactggtgg gcaatcattt
     1681 tgcatttgaa tggctgcagc cgttgcgtga tgatttcaat ggctatcaac ttgagcgtca
     1741 gggggcctta tccgcaacgt ggcattatag tttttgatgg tgatgaaact ttatcaatat
     1801 acatttaagg caatgggcac gccttgtgag attcagcttt atggagccgt ggaaagcgag
     1861 gtatcccagg ttgccgaatc cgccattgcg gatgtccgtc gattggagac cgcgtattca
     1921 cggtatcgtg atgatagctt tttatcagag attaatcgtg ttgctgctca tggtggatcg
     1981 atccaggtag attcggaaac tgcaggatta ttggattacg cagaagcttg ctaccatgcg
     2041 agtgatgggc tattcgatgt tacctcaggg atattaagac aagcctggaa ttttaaatcg
     2101 aatcaattgc cggatgagca aacgatacag tctttattgg atcgagtagg ttgggaaaaa
     2161 gtgcactggt tatcgcccaa tcttgaattt cccgttgctg gaatggaaat cgatttaggg
     2221 ggtgttgtaa aggaatatgc gacagatcgt gctgcatcga tttgtttgga tcgtggaata
     2281 caaaatggta tggttaattt agggggagat attaaagtca tcggtcctca tatcgatgga
     2341 agtccctgga aaattggtat aagccaccca cgacaacccg caactttgct gcatacatta
     2401 gcgttacatt cgggtgcctt ggccagtagc ggagattatg agcgatgtat tacgattaat
     2461 ggtatccgtt atgggcatgt attgaatcct aaaacgggtt ggccagttag aaaaatggct
     2521 gcagtaagtg tcgttggtga tttctgcgtg gttgccggaa gcgcctcaac aatcggtatg
     2581 ttaaaagatg aagaagcgaa ggattggctt gctatccttg gcttacccca tttatgggtt
     2641 aatgtttcgg gtgaagtagg tgggtcgctg ctggatacag tgcaagtgac taaaatttat
     2701 aaccacgatg aagcgcaacg atacccgtac tgagattaaa atattcaacg cgttcaaagc
     2761 caacgtcttg catgagttgt tttaactctt cttgagaagg gtgcatgcga atcgattctg
     2821 cgagatatcg ataactctct tcgtcttgcg ctacaaattt tccaatgaaa ggcaacgcct
     2881 tgaatgagta agcatcataa agcggttgta gtggtttcca gattttggaa aattccaata
     2941 cgatgactga accccctggg cacagtacgc gtaacacttc cttgagtgca atatccttgt
     3001 gagtcatgtt tctaagacca aaggcgacac atacatgatc aaaatagtta tcaggaaatg
     3061 gtagtttttc tgcatcgcat tgagtggcag ggactgcttt gccgtcgttt aacaaacgat
     3121 cacgcccgat ggtcagcatg gagttattaa tatcagttag ccatacttga ccggattcac
     3181 cgacttgctc aagaaataaa gctgacaaat cagccgtgcc gcccgcaata tcaagtatac
     3241 gatctccttt tttagcgcca ctggcatcaa ttgcaaaacg tttccagagt cgatgtaggc
     3301 ccattgacat gagatcattc attaggttgt agcgagcggc aacggagtga aaaacatcgg
     3361 caactttatg tgctttctca gattcttcaa ctgttttaaa accgaagtga gtcgtattgg
     3421 tcatgtctat ttaaatataa gggagcccaa ggaaaagcgg acgaattaat cgtgcttgat
     3481 acctgcagct tcgagtcgtt gaagataatg atgccacatt ttttcttgat tgaccccgta
     3541 attataaagt aaatcccacg aatagattcc cgtattatgc ccatccgaaa aatgcggttg
     3601 aatggcatat tgcccaatgg gttcgatttt cacaatggtg acatcttgct tgtcaatttg
     3661 aagtacttcc tgccctggcc catggccttg aacttcagca gaaggtgagt aaacccgcaa
     3721 gaattcactg gataactgaa atttttggtt gttattaaac gtgatttcaa gcaatttcga
     3781 ttgtttgtga aattttattt ctgttggagt tggtgtgttt ttattcaagc cagccataaa
     3841 aaattaataa ttagttttag atgacaaatt tgtatttgtc acaaagttgc aatgttagct
     3901 ttatactatt gagagactta ctaaaaactt atttttgagt ggagtcaatc gatggctgca
     3961 acaattttaa ttgtagaaga tgaacctgcg atacaggagt taatttctta taatctgcgc
     4021 aacacgggct ttattgtagc gtgtgcggga agtgccgagc aagcaaagat catggtaaat
     4081 gacgtgctac ctgatttaat tttattggat tggatgcttc ctgggacgaa tggtattgat
     4141 tttgcgcgat atttgcggca aggtgcccgc accaaagcga ttcccattat catgctgacg
     4201 gctcgggttg atgagagtga taaagtatca gggttagaag tcggtgcaga tgattatgta
     4261 atcaaaccgt tttcaccgcg tgaactggtt gcaagaatta aagcggtgtt acgtagacgg
     4321 ctaccagagg cctcagatga aatcgttgaa acaggggggt taaagttgga tccggccact
     4381 catcgtgtga tggtgaccga tagcgcagat aaatcaaaat caatcgaagt caatcttggg
     4441 ccgacggagt ttcgattatt gcattattta atgacgcata cggaacgtgt acatacgcga
     4501 ggacagttat tggatcgcgt ttggggtgat catgttttcg tagaagatcg aacggtcgat
     4561 gttcatatac gtcgcttacg tcgtgctttg gaagatgtag gtaaggatga tttgattcaa
     4621 actgtgcggg gtgctggtta tcggttttct taccaatgtg aagttgatac tcattaatcg
     4681 gtatatcatt taataaagta cggcggttgg ttgaatgaaa tagtgtacga ccgacttaga
     4741 cttaagctac tatatagcgc cttagtcaat ttaagtcgtc ttgataattt ttagcgttaa
     4801 atgctcattg tttagaagtc attgcttgtc aaaaatatga gcaatgatct acctgcattt
     4861 cagatcgatt tcttagctat cctaacgtca gaataaatgg aaattcgaga ttctgtaacc
     4921 aaaccctacc tttctttgga atcaatacgt gtttgagata gggcgtcgat ttgtcgggat
     4981 cgtattgctg atcatcgttt ccgtaatact tggatttttc tttgatgaga cggtagcact
     5041 gcttttctta agttcaatct tactgatcat tgtcagttac catatcattt cattggcttc
     5101 actagaccgt tggctacagc gctatgatcc gtcttctcaa gtaatggctt ccgttcctaa
     5161 taactttggt gcttggggcg atgtttttgt gcggttgacg cgctatatgc ggcgttatta
     5221 tcgtagccga caaagcttga gccgggcatt agagcgcctg caaagggcca cttcagccat
     5281 gccggatgga attgtgattt tggataagat cgaccggatt gaatggtgta atcctgttgc
     5341 agagcagcac ctaggattag acttaaaaat ggatattggg cagcatatta cgcatttagt
     5401 gcgacaaatg cagtttgtgg agtatctaac ttcgggggat tttagtaaac cagttataat
     5461 taagcaatcg cgctatcagg agatcatgct atcgctacag ctcgttcctt acggtgatga
     5521 ggaaaaattg ttgatcagtc gagacattac gcgatttgag aagatggaaa tcatgcgtcg
     5581 ggattttatt gccaatgtct cgcacgagct cagaacgcct ctgacggtaa tcaatggatt
     5641 tcttgaaatg cttcaagata acggaatgaa agatagcgat atggagaaac atgcgctcgg
     5701 attgatgagc cagcaaagtc ggcgcatgca acgactggtc gaggacttgc taacgttatc
     5761 gcggttggaa aatggacaaa atgttttgcg tgaggaagaa gtggatatga aagagctact
     5821 ccagaatctc cttcatgagg ctcaatcttt aagtgcagcg cgccatacca tcaccctaca
     5881 gttaaaaaaa gatgatatca atgtgcttgg tagcgcggat gagttgcgaa gtgcgttggg
     5941 gaatttagtc agtaacgctg tgcgttatac cccggatggg ggtgatatcg ttttatcttg
     6001 gggagaagag aaaggtttat tttatgttcg agatagtggc atcggtatcg aacctcagca
     6061 tataccccgc ttgactgaac gattttatcg aatcgatcgg agtcgttcac gtgaaactgg
     6121 agggactggg cttggattag caatcgtcaa acatattttg aatcgacatc aagcgcgaat
     6181 ggatattatc agtgaattag gaaaagggag tacgtttaaa gtgtggtttc cgctaaaacg
     6241 attacaagca acaaccaaaa atagcttacc agaagtggtg gaagcacagt tggaacatag
     6301 ccaattcatg ccaccgcaac aataataatt aattaaccgt taggtggaaa tgactgagat
     6361 gtggggaatt agggtgattt ggattaattc cctcgatggt aggcgcgatt ggactcgaac
     6421 caacgacccc caccatgtca aggtgatgct ctaaccagct gagctacgcg cctaagcgtg
     6481 cttgcattct aactgaaaag aaaatccgct tcaaatttat tgtttaaaca tcaatgaaat
     6541 cgacaaaata taatcctccc cgatggcttt ggggcggtaa tgcacagact atttatccct
     6601 atctaatgag ccagccagca tcgattccct accggcgaga gcgatgggag cttgatgatg
     6661 gagattttat tgatgtggat tggttagatg gcgagggaaa taagccactt gttgtattgt
     6721 ttcatggatt agagggtgga tcgcgaagcc attacgcgtt gagtatgatg cgctatttac
     6781 agagctgcca gtggaatggg gtcgtcattc attttcgtgg atgctcaggt tatccgaatc
     6841 ggttaccgcg tgcttatcat gcgggagatt ctgcagaaat tgactggatg ctaaagcgta
     6901 ttgcttcaat acagtcctca caaccacaaa aatcaaatcg tactatctat gcaatgggcg
     6961 tttctctggg aggcaatgcg ctattaaaat ggctagggga gcaagggaag atagcgaatc
     7021 agcacattgc agctgtggca gcgatatcag ttcccatgga tttggttgct gctggtaatg
     7081 tattggataa gggatttaat cagctttata cgcggcattt tttaagttcc ttaaagcaaa
     7141 aaatagtgga gaagcaaaag aaatttccag gcttatttga cattaaagca gttactcaat
     7201 gtgcaacgtt atatgaattc gataatttgg taacggcgcc cttacatgga tttcgtgata
     7261 ccgatgaata ttggacgaca tcgagtagta aaccctggtt acgggcgatt gaagtaccca
     7321 cgctcgtact gaatgcgaaa aatgatcctt tcttaccagc acatgcatta cctgagtatt
     7381 cagcggtttc ttcggcagta acgctggagt atcccgaaca aggagggcat gttggtttct
     7441 tacatggccc atttcccggt aaattgatgt ggctgcccga acgtatactt cgtttctttg
     7501 aaggggtaaa ttgcaataat cagtgactta taagtttctt ttaccaaagt ttatgactta
     7561 gtattatcat gggatctaac ggttacagaa gttatggccg tgaatatcga tattgatagt
     7621 caatctaccg aaggggaggt gatatgctaa ggttcgatcg aattactttg atattactga
     7681 ttttcttttt ctcagaagcg tatgcggtag gatgggaaaa gatagaagta tccaacactg
     7741 agttaacgat actggatgat aacgggcagt ataaagatat caagccaggt tgtgcattta
     7801 gttatttacc tgatgaagca ggattaccga ataagccatt tcatttttat taccggaaag
     7861 ggactaaacc acaaacactg attttcctta atgggggagg cgcctgctgg aatgatgcaa
     7921 cctgtacaac atctctaacg gttccagttt caagcacaac acggcccgcc tataatcctt
     7981 ccatggattt tgagaatgat ccagaaagtg tagggggaat acttgatttt gctcgtgcag
     8041 ataacccgct gaaagattgg aatatggtct ttattcccta ttgtacgggt gacgcacata
     8101 ttggttcgaa cgataaagtt tatgctgatc ctttaggaat cattaacgct ggtaatccta
     8161 tcatggtaca gcaccggggt ttcgataatt ttatggcggt gcgcgagtgg ttaaagcata
     8221 gaccggatcg attcaatact gaaaaagtgc tagtagctgg gtcaagtgcc ggcgcatacg
     8281 gagcactaat gaatttttct cgattacatc gtatttatcc gagcatgaca aaaatttctc
     8341 tattgtctga tgcgggtgcc ggtgtattta ccaataattt tttgaatacc gtttttgagc
     8401 caaacggccc atggggaaca gagaatacct tagcgacttg gataccggga atcaatcaga
     8461 ttagtagcta taacgatcta aatt
//
LOCUS       DAOQKQ010000113         8347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_4134_length_8347_cov_5.959117, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000113 DAOQKQ010000000
VERSION     DAOQKQ010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 8347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8347
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4134_length_8347_cov_5.959117"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2248
                     /locus_tag="PKZ49_08380"
     CDS             <1..2248
                     /locus_tag="PKZ49_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HND36615.1"
                     /translation="LPKEMSGIDLNGTRVPFSAIYDSNSLRVDLNERMPGGFGGVAEY
                     GITVRWDKNFLQFIRLLLERRNEFALFGGVRFGGTLTADDALNLGFDHIALAAGAGRP
                     TVLDLPNGLARGVRAASDFLMALQLTGAAQTDSIANMQLRLPVVVIGGGLTAIDTATE
                     SLAYYPIQVEKFLQRYETLAAVQGEDSIQRSWDEEEREIATEFLMHARAIRAERLQAQ
                     KEGRDANIIKLLQAWGGATLAYRKRLVDSPSYTLNHEEVEKALEEGIWFAEGLTPVRI
                     ELDQWEHTRSIRFSVQSMDENGKWHQSKEVELPARAILVAAGTQPNTVLAREDEKYFK
                     LDGRYFAACDERGNPVSITKGNPKPETPAVLLSRCSDGRFISFFGDLHPSYSGNVVKA
                     MASAKQGYSVVSGVLSQVKPVNPQVDHEFIDNLNRQLRAAVYKVERLTPNIVEVIVHA
                     PLAAKHFHPGQFYRFQNFATLSKIAVNTRLAMEGIALTGASVDNSKGLVSLIALEMGG
                     SADLCTLLNPGDPVILMGPTGTPTEIFQNETVVLVGGGLGNAVLFSIGAAARAAGCKV
                     LYFAGYKKLVDRYKVAEIEAAADTIVWCCDEQPGFEPSRQGDFSFVGNIVQAMVAYGS
                     GALGSQSISLKDADRIIAIGSDRMMAAVGIARHQQLKPYLKDSHFAIGSINSPMQCMM
                     KEICAQCLQPHKDPKTGETTYVFSCFNQDQELDSVDFGGLASRLKQNSVQEKLTRHWI
                     SHCLQHLN"
     gene            2380..3519
                     /locus_tag="PKZ49_08385"
     CDS             2380..3519
                     /locus_tag="PKZ49_08385"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HND36616.1"
                     /translation="MHIGIPAEIFAGETRVAATPETVKKYTAKGLHTVLVQTGAGAGA
                     SIPDNAYQEAGAVIVSDAAQVYSQAQIVLKVRGPTADELPMLRKDLVLIGLLSPHHTD
                     GISTLAGRGVTAFAMEKLPRISRAQSMDVLSSQANIGGYKAVIIAADTYQKFFPMLMT
                     AAGTVKAARVLVLGAGVAGLQAIATAKRLGAVIEAFDVRPAVKEQVESLGAKFVEVPL
                     TDEEKAKAKAKAETAGGYATEMSDDYKRRQGELINERAAAADIIITTALIPGRPAPVL
                     IKEETVKAMKPGSVIVDMAVEAGGNCPLSELNKTVTKYGVHLIGVANLPGLVAADASA
                     LYARNLMNFLNLMLDAKTGELNINLEDEIIAGTMVCSGGDLAKKA"
     gene            3566..3874
                     /locus_tag="PKZ49_08390"
     CDS             3566..3874
                     /locus_tag="PKZ49_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-translocating transhydrogenase family
                     protein"
                     /protein_id="HND36617.1"
                     /translation="MIGDIDPTIIHLTVFVLAVFVGYHVVWNVTPALHTPLMSVTNAI
                     SSIILVGAMLAAGPLEADWGVWLGAIAVVLASINVFGGFLVTQRMLEMFRKKDKKGNA
                     "
     gene            3875..5254
                     /locus_tag="PKZ49_08395"
     CDS             3875..5254
                     /locus_tag="PKZ49_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HND36618.1"
                     /translation="MSANFIALAYLVAAVFFILALKGLSSPLSSRRGNLFGMVGMAIA
                     VATTLTITQNWMLILGCIAIGGIIGSIVANRVQMTAMPELVAFMHSLVGLAAVFIAIA
                     AVNNPVSFGLPETLPMGSKLELFLGTFIGAMTWSGSVIAFLKLSGKMSGAPVIFSGQH
                     AINLLLAIVMIGFGLWFFFSETTNWTAFIAMTAIAFVLGFLIIIPIGGADMPVVISML
                     NSYSGWAAAGIGFSLGNPMLIIAGSLVGSSGAILSYIMCKAMNRPFLSVILGGFGGDA
                     GSQAASDGTQKTYRSGSAEDAAFLMSNADSVIIIPGYGLAVARAQHSVKELAEALHKK
                     GVNARFAIHPVAGRMPGHMNVLLAEAEIPYEQVQEMEEINSDFSTTDVVLVLGANDVV
                     NPAANVPGSPIYGMPILEAHKARTVIVVKRSMAAGYAGLDNDLFYMDKTMMVFGDAKK
                     VVEDMVKSI"
     gene            complement(5297..6121)
                     /locus_tag="PKZ49_08400"
     CDS             complement(5297..6121)
                     /locus_tag="PKZ49_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HND36619.1"
                     /translation="MTNSQKATRNKYYLNNHCPDCGSNRIHRSRLRPGERSPSNWNLM
                     PMRCRDCKARFWVKNRDAYIVASVVFICSLLLITFIWIIISLDEISIDGKKFDKQLAS
                     NQSTANQSSNSFPTTYPKAAIEATNTQQNGVVNQSMPAETQYSSDDAHHHMINLFLNR
                     ANEGNAEAQHQLGQLYLTGNGTLQDFEEAVKWFSLAAEQNHAPAQYELGLLYHIGQGV
                     NIDNEKSYMWLNLSTAGGYEQAILARDKVARSLNPAQLVQAQKAAKEWLNTRINKK"
     gene            complement(6200..6976)
                     /locus_tag="PKZ49_08405"
     CDS             complement(6200..6976)
                     /locus_tag="PKZ49_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HND36620.1"
                     /translation="MPLSAVLFDVDGTLADTERDGHRLAFNSAFKELGLNWNWDIELY
                     GKLLQITGGKERIRYYIDHYSPEISKNTNLTDWVAELHKTKTKYFLQLLSEGKIPLRP
                     GVARLIQELRNNAIKIAIATTTTPENVYALLKSTLGEDSPSWFDVIGAGDIVPHKKPA
                     PDIYHWVLDRLDLTPAECIAIEDSENGLRASLTANITTIVTTSEYTHSHDFKGAAVVL
                     TDLGEPNKPATILANSFFPEPKSASIDISFLETVMNNQCK"
     gene            complement(6998..8035)
                     /locus_tag="PKZ49_08410"
     CDS             complement(6998..8035)
                     /locus_tag="PKZ49_08410"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HND36621.1"
                     /translation="MAITTSIAKTDLLKEQIFKAARLLQTGNNIVFPTETVYGLGADI
                     THLSAVNRIFQIKERPTNHPLIVHFADLGQISHWAENIPSQAKKLIEYFWPGPLTIIL
                     PKSRHVPYCVTGGQDTVGLRIPDHPVAIALLKALGPQKAVVAPSANRFGCLSPTAALH
                     ISKEISAEVSMILDGGPCKIGLESTIISFCDLKPVLLRPGGIPLNAIEEILNQNIIVS
                     VNHSNNLRASGTLASHYAPRTPLEVWPTETLQHRVDELLNQKYHVAILSWSEAFYSQH
                     QTTEKIIKSLKHHRMPSDPFDYGYHLYAKLHHLDDEGYDRLLVEMPPNLPDWLAVHDR
                     LQRASHHFAIR"
BASE COUNT         2314 a         1496 c         2022 g         2515 t
ORIGIN      
        1 attacctaaa gaaatgagtg gcattgatct caatggtacg cgtgttccat tttctgcgat
       61 ctatgatagt aactcgctac gggttgatct gaatgaacgc atgccgggtg gttttggcgg
      121 tgttgccgag tatggtatta ccgttcgatg ggataaaaat ttcttgcagt ttattcgatt
      181 gttattagaa cgtcgtaacg agtttgcgct ttttggcggg gtacgctttg gtggaacgct
      241 gacggcggat gatgcgctga atctgggttt tgaccatatt gcattggcag caggggcagg
      301 tcgtccgaca gtactggatt tacccaatgg attggcaagg ggtgtgagag ccgcatcgga
      361 ttttttaatg gcattgcaat taacaggggc ggcacaaaca gactcgattg ctaatatgca
      421 gttacgtttg cctgttgtag tgattggcgg gggattaacg gctattgata ctgcgactga
      481 gtcgcttgca tattacccta ttcaagtaga gaaatttttg cagcgctatg aaactcttgc
      541 tgcagttcaa ggggaagatt ctatccaacg gagttgggat gaagaagaac gagaaattgc
      601 aacggaattt ttgatgcatg cgcgtgcaat tcgagcagag cgacttcagg cgcagaaaga
      661 agggcgcgat gctaatatta taaagttatt acaagcttgg ggtggcgcaa cactcgctta
      721 tcgtaaaaga ctcgttgata gtccttctta taccttaaac catgaagaag ttgagaaagc
      781 attggaggaa gggatatggt ttgctgaggg tctgacaccg gtacgaatcg aattagatca
      841 atgggaacat acgcggtcaa ttcgattttc tgttcagtcc atggatgaaa atggcaaatg
      901 gcatcaatcc aaggaagttg aattgcctgc tcgtgctatt cttgttgcag caggaacaca
      961 gcctaatact gtattagctc gagaagatga aaaatatttt aagctcgacg gacgttattt
     1021 cgctgcatgc gatgaaagag gtaatccagt tagtatcacc aagggtaacc ctaaaccaga
     1081 aacgccagca gttctgttaa gccgttgctc agatgggcgt tttattagtt tttttggcga
     1141 tcttcatcca tcgtattctg gcaacgttgt gaaagcgatg gcgagtgcta agcaaggtta
     1201 ctcggtagtg agtggggttt taagtcaagt taagccagtg aatccccaag tagatcatga
     1261 atttatcgat aatttgaatc gccaactcag ggcagcagta tataaggtag aacggttgac
     1321 gcctaatatc gtcgaagtaa tcgttcatgc gccattagct gcgaaacatt ttcatccagg
     1381 tcaattttat cgtttccaaa attttgcaac gttatctaag attgcagtaa atacgcgtct
     1441 agcgatggaa gggattgcct tgacaggggc ctctgttgat aattcgaaag gacttgtgtc
     1501 attaatcgca ttggagatgg gcggatcggc tgatttatgc acgttattaa atcctggtga
     1561 tccggtaatt ttgatgggcc ctactggcac acctactgag atattccaga atgaaaccgt
     1621 agttttagtc gggggagggc tgggtaatgc agtcttattt tcgattggag cagcagcgcg
     1681 tgctgcaggc tgtaaggttt tatactttgc aggttataaa aagctagttg atcgttacaa
     1741 agtagctgaa attgaagcag cagcagacac cattgtatgg tgttgtgatg aacagcccgg
     1801 atttgaacct tctcggcaag gtgattttag ttttgttggt aatattgtac aggcgatggt
     1861 tgcttatggt agcggtgctt taggttctca gtctatatcg ctgaaagatg cggaccgaat
     1921 cattgcaatt gggtctgatc ggatgatggc cgcagtgggt attgcgcggc atcaacagtt
     1981 aaaaccttat ttgaaggata gtcactttgc aattggttca attaattcgc ccatgcaatg
     2041 catgatgaaa gaaatctgtg ctcaatgctt gcaaccgcat aaagacccta aaaccggcga
     2101 aacaacttat gttttttctt gttttaatca agatcaggaa cttgattcgg ttgattttgg
     2161 aggattggct tcacgtttaa aacagaatag cgtgcaggaa aaattgacga gacattggat
     2221 tagtcattgc cttcagcatt taaattaaaa ataaaaaaga taaatacagt gaaaaataca
     2281 attgaatacc gatacttgga ttgatttata atgatacatt caagacaatt gaaagtattt
     2341 ttaattattt ggtttgaaag aaaataaggg agataatcaa tgcacatagg catacctgca
     2401 gaaatttttg cgggagaaac gcgggtcgca gctacgccag aaacagtaaa aaaatatact
     2461 gctaaggggt tgcataccgt attggtccaa actggcgcag gagcgggtgc tagtattcca
     2521 gataatgctt accaagaggc aggcgcagtg attgttagtg atgctgctca agtgtatagc
     2581 caggcccaaa tcgttctgaa ggttcgagga ccaactgctg atgaattgcc tatgttgcgc
     2641 aaagatttag tgctaatcgg gttattgtca cctcatcaca ctgatggaat tagcactctg
     2701 gcaggacggg gtgtaacagc atttgcgatg gaaaagttgc cacgaatttc gcgagctcaa
     2761 agtatggatg tgctttcatc gcaagcaaat attggtggtt ataaggcagt tatcatcgct
     2821 gcggatacct atcaaaagtt ttttcccatg ctcatgaccg cagcgggaac agttaaggcg
     2881 gcaagagttc tggttctagg agccggtgtt gctgggttgc aagcaattgc gacagcgaag
     2941 cgcttgggtg cagttattga agcattcgat gtacgtccag cggtaaaaga gcaagttgaa
     3001 agtctaggtg caaaattcgt tgaagtccca ctgacagatg aagaaaaagc aaaagcaaaa
     3061 gcaaaagcag aaacagctgg tggctatgca accgagatgt cggacgatta taagcgtcgg
     3121 caaggggaat taattaatga acgagcagct gcggcagata tcatcattac cacggcttta
     3181 attcctgggc ggccagcgcc tgtattgatt aaagaagaaa ctgtaaaagc aatgaaaccg
     3241 ggctctgtga ttgtcgatat ggctgttgaa gcgggaggga attgtccctt atctgagctg
     3301 aataaaacgg ttaccaagta tggtgtgcat ttaatagggg ttgctaattt accaggttta
     3361 gttgctgcgg atgcgagtgc tttatatgcg cgtaacttaa tgaatttctt aaatctgatg
     3421 ttggatgcaa aaactggtga actcaatatt aatcttgaag atgaaattat tgcaggaact
     3481 atggtttgct ccggcggcga cttagctaaa aaagcgtgat acttgaacaa gttattttaa
     3541 gtgataaatc ttatgaggaa taaacatgat tggtgatatt gatccaacga taattcatct
     3601 tactgtattc gtgctggctg ttttcgttgg ctaccatgtc gtatggaatg tgacccccgc
     3661 tttgcatacc cctttaatgt cggtaacgaa tgcaatttcg agcattattt tagttggcgc
     3721 gatgcttgct gcaggcccat tagaggctga ttggggagtc tggttgggag cgattgctgt
     3781 ggtgcttgct tcaattaatg tatttggcgg tttcctcgtt acgcagcgta tgttggaaat
     3841 gtttagaaaa aaagataaga aaggaaacgc ataaatgtca gctaatttta ttgctctagc
     3901 ttatctcgtt gcagcagtat tttttatatt agccctaaag gggctaagct cgcctttatc
     3961 atcccgtcga ggtaacttgt ttgggatggt tgggatggcg atcgcggttg caacgacctt
     4021 gacgattacc cagaattgga tgctgatatt gggttgcatt gcaattggcg gtattatcgg
     4081 ttcaatcgtt gcgaatcgtg ttcagatgac tgcaatgccg gagttagtgg cgttcatgca
     4141 ttcattggtc ggtctagcgg cggtgtttat tgctattgct gcggtcaata acccggtatc
     4201 ctttggtttg cccgagacac tgcctatggg tagtaaatta gagctatttc tcggtacttt
     4261 catcggcgca atgacttggt caggttctgt catcgcgttt ctgaaattat ctggaaaaat
     4321 gagcggggct cctgtgattt tttcaggaca gcatgcgatt aatttgttgc ttgctatcgt
     4381 gatgattggt ttcgggttgt ggttcttttt tagcgagaca accaactgga cagcatttat
     4441 tgcaatgacg gctatcgcct ttgtgcttgg attcctgatc attattccaa tcgggggtgc
     4501 tgatatgccg gtcgtaatat caatgctgaa ttcctattca ggctgggcgg cagccggaat
     4561 tgggttctca ttaggcaatc cgatgctgat aattgcaggt tcattagtgg gtagctcagg
     4621 tgctattctt tcttacatca tgtgtaaagc gatgaatcgc cccttccttt ccgtcatact
     4681 gggtggtttc ggcggagacg ctggtagtca ggctgcaagt gatggcactc aaaagaccta
     4741 tcgcagtggt agcgctgaag atgcggcttt tctaatgagc aatgctgata gcgtgattat
     4801 aattccgggc tatggcttgg ctgtggctcg tgcacagcat tctgtcaagg aattggccga
     4861 ggcgcttcat aagaaagggg ttaatgcgcg tttcgccatt catcctgtgg caggtagaat
     4921 gccgggccac atgaatgtat tgttggctga agctgaaatt ccttatgagc aagtccaaga
     4981 aatggaagaa attaatagcg atttctcaac tacagatgtt gtattagtgc taggagcaaa
     5041 tgatgtggtt aatcctgcgg caaatgttcc agggagtcca atctatggca tgcctattct
     5101 agaagcacat aaagcacgta ctgttattgt agtaaaacgc agcatggcag cagggtatgc
     5161 gggcttggat aatgatttgt tctatatgga caaaacgatg atggtatttg gcgatgcgaa
     5221 gaaagttgtt gaagatatgg ttaaatcaat ttagcctttg aggatgcgac cagagttgct
     5281 tcattgaaat atcgattcat tttttgttga ttcttgtatt gagccattct ttagcagctt
     5341 tctgtgcttg aacgagctgc gctggattca atgaacgagc tactttatca cgtgcgagaa
     5401 tagcttgttc ataccctcca gcggttgaca aattgagcca catatagctc ttttcattat
     5461 caatattgac accttgtcca atatgataga gcaagcctag ttcatattgg gcgggtgcat
     5521 ggttctgttc tgcagcgaga ctgaaccatt tcaccgcttc ttcaaaatct tgcaatgttc
     5581 catttcctgt caagtatagt tgaccaagtt gatgctgagc ttccgcattg ccctcattag
     5641 ctctatttag aaaaagatta atcatgtgat ggtgagcatc atcagaacta tattgagttt
     5701 cggctggcat tgattgatta actacaccat tctgttgagt gtttgttgct tcaattgctg
     5761 ctttaggata ggttgtggga aaactattag atgactggtt tgcagtagat tgatttgaag
     5821 caagttgctt atcaaatttt tttccgtcta tcgatatttc gtcgagtgaa ataatgatcc
     5881 agataaatgt aattaagagc aaactacaga taaaaacaac ggatgcaaca atataagcat
     5941 cacgattttt aacccagaaa cgtgctttac aatcacgaca tctcatcggc ataagattcc
     6001 agttagaggg tgaacgctct ccaggtctta atctggatcg gtgaatacga ttactaccac
     6061 aatcaggaca gtgattattc aagtagtatt tattgcgagt cgctttttgt gaattggtca
     6121 tttactatga gaaatcaatt atctaaatcg cttttacttg gcgcggctaa gcaaaaagaa
     6181 ggtgaataat aaaaaattac tacttgcact gattattcat aactgtttct aaaaaagaaa
     6241 tatcgatcga cgctgatttg ggttcgggaa aaaaagaatt tgcaaggatg gtcgcaggct
     6301 tattaggttc tcctaaatcc gttaaaacta cggccgcacc tttgaagtca tgtgaatgtg
     6361 tatattcact cgttgtaaca atagtagtga tattcgctgt aagcgaagct ctaagcccat
     6421 tttctgaatc ctctatagcg atgcactcgg ctggcgttaa atcgagccta tcaagaaccc
     6481 aatgataaat atcaggagcg ggtttcttat gagggacaat atctccagcg ccaataacat
     6541 caaaccaact tggtgaatct tcacctaagg ttgactttaa aagtgcataa acattttctg
     6601 gggttgtagt tgtggcgatc gctattttaa tagcgttatt gcgcaattct tgaattagcc
     6661 tagcaacgcc aggacgtagg gggattttac cttctgacaa taattgtaag aaatattttg
     6721 ttttggtttt gtgtaattcc gcaacccagt cagttaagtt ggtatttttc gatatttctg
     6781 gcgaataatg atcaatgtaa tagcgtatcc tttccttgcc accagtaatt tggagcaatt
     6841 taccatagag ctcgatatcc caattccaat tgagtccaag ttctttaaaa gcggaattga
     6901 aagcaagacg atgtccatct cgttctgtgt cagcaagggt tccatcaaca tcaaataaaa
     6961 ctgcagataa tggcattaat tattaccttt tattatatta gcgtatcgca aaatgatggc
     7021 ttgcacgttg taatcgatca tgaacagcaa gccaatcggg aagattggga ggcatttcga
     7081 ctaataatcg atcatagccc tcatcatcta agtgatgaag tttggcatat aaatgatagc
     7141 cataatcaaa tggatcagaa ggcattcgat ggtgtttaag ggacttaatt attttctcag
     7201 tggtttgatg ttgtgaataa aatgcttcag accaagagag aatagcgacg tggtattttt
     7261 gattcaatag ctcatcaact cggtgttgga gagtttctgt tggccaaact tcgaggggag
     7321 ttctgggtgc gtaatgggac gcaagggttc ccgaggctcg taaattattg ctatgattga
     7381 ctgagacaat tatattttga ttaagtattt cttctattgc attcaaagga atgcctcccg
     7441 gtctgaggag aacaggtttt agatcgcaaa aactgataat cgtactctct aaccctattt
     7501 tacaaggccc cccatccagt atcatactta cttcagcact gatttcttta ctgatatgaa
     7561 gcgctgctgt tggacttaag cagccaaatc gatttgctga aggggctacg actgcttttt
     7621 gagggcctaa tgcttttaat aaagcgatag caactggatg atcaggtatg cgcaaaccta
     7681 cggtatcttg accacccgtt acacaataag gtacgtgccg acttttggga agaattatcg
     7741 taagggggcc tggccaaaaa tattcgatta gtttcttggc ttggctgggt atattttctg
     7801 cccaatggct tatttgcccc aaatctgcaa aatgaacgat cagagggtga ttagtgggac
     7861 gttccttgat ttgaaatatt cgattaactg cgctgaggtg agtgatgtca gctcctaacc
     7921 cgtatactgt ttcagttggg aaaacaatgt tattgccagt ttgtaacagt ctagccgctt
     7981 tgaaaatttg ttctttaagc aagtcagttt tagcaattga cgtcgtaata gccatttgtt
     8041 gtcactaaat gagaatgttg tatttaattc cttgtgtcag ccttatttta atctaattcc
     8101 tcggatgaat ggattatgga ttaagaacgc taaaagtaat tttaagaaaa atattcttct
     8161 tggtggttca ttttctcttg aaagaaataa ttacaaagaa aagttaaatc tttaaaaaaa
     8221 gaattttaac cctttgtttt ggaaaagcta taaagcccta ttctagaggt gattatccta
     8281 aattcccgga ggggtgatgg aaaaactact tgactaaaat ggataaggtg actagcctgt
     8341 agggtgt
//
LOCUS       DAOQKQ010000114         8235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_4207_length_8235_cov_6.215037, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000114 DAOQKQ010000000
VERSION     DAOQKQ010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 8235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8235
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4207_length_8235_cov_6.215037"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /gene="gcvPB"
                     /locus_tag="PKZ49_08415"
     CDS             <1..1016
                     /gene="gcvPB"
                     /locus_tag="PKZ49_08415"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HND36622.1"
                     /translation="ARGDTKRTEIIIPDAAHGTNPATAVMCGYKVVEIATDKEGDVDM
                     DALKAAVGPQTAGLMLTNPSTLGVFEKNVSEMSRIVHEAGGLLYYDGANLNAVLGKVK
                     PGDMGFDVIHINLHKTFSTPHGGGGPGAAPVGVAERLLPYLPIPVVAKTEKGNYRWLT
                     EKDLPKSIGRLSAHMGNAGVLLRAYIYVRLLGFDGMHRISEFATLNANYLMAELREVG
                     FEIAYPTRRASHEFIVTLKEIKDRTGVTAMNLAKRLLDKGYHAPTTYFPILVPECLLI
                     EPAETESKETLDAFVAAMKEILVEIKEQPDMVKGAPHNMPLRKLDDVKAARELDLTWK
                     PAS"
     gene            1105..2040
                     /locus_tag="PKZ49_08420"
     CDS             1105..2040
                     /locus_tag="PKZ49_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HND36623.1"
                     /translation="MRTLICGSIAYDTIMVFQDHFKHHILPEKIHVLNVAFLVPEMRR
                     EFGGCAANIAYNLKMLGEDPLIMATVGDDFQPYAYRCEKVGFDKTHIRQIDDTFTAQA
                     FITTDLDDNQITAFHPGAMNFSHHNSVKEAKHVSLGIVAPDGRDGMIAHAREFQECGI
                     PFIFDPGQGLPMFSGDELVDFISKADYVTVNDYEAELLLERTGYSLGKLASLVKALIV
                     TKGAQGSVIYTGGHHIEIPSVQPKAIVDPTGCGDAYRAGLLYGIAHGLDWYATGKLAS
                     LLGALKIAYRGGQNHEFDRSAIDQHYFEAYGNRIF"
     gene            2279..2956
                     /locus_tag="PKZ49_08425"
     CDS             2279..2956
                     /locus_tag="PKZ49_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HND36624.1"
                     /translation="MAKKWAQRFLRILNIKLHYHGSLPSDDQARVILAANHISWLDVI
                     AILAIYPVRFVAKSEILDWPIIGKLSANAGTIFVAREKRNDTLRVNQFLSEILIAGDR
                     IAIFPEGTTSDGTHLLHFHASLLQFAVASKGLVYPVAIHYRDAMNQPTTLTAYVDVSI
                     TQSLQLILQQPSCHVELNFLTPLQGEEKNRRELARLTEHAISHQLALPVVRKGSEKSS
                     DLPSESQ"
     gene            complement(2865..3704)
                     /locus_tag="PKZ49_08430"
     CDS             complement(2865..3704)
                     /locus_tag="PKZ49_08430"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HND36625.1"
                     /translation="MATYAIGDLQGCFKQLQHLLEFIAFSPSRDRLWFVGDIVNRGPD
                     SLSLLRFISQLGDSATTVLGNHDLHLLLVAEEITSQRSGDTLQAILNAPDRNQLLYWL
                     RHQKMLHVEDDYVLVHAGLLPSWPASFALELAQEVEATLQSNDYTDLLLHLYGNQPDQ
                     WHDDLKSYDRLRLIVNALTRLRICTKEGKMDFSYKGPLETIPEGYFPWFDVPSRQSQK
                     STIIFGHWSAIRLQLRHNLIALDTGCLWGAELTAIRLEDRKIFQIPCVQPVKRAGVKS
                     HAR"
     gene            complement(3722..4531)
                     /gene="dapB"
                     /locus_tag="PKZ49_08435"
     CDS             complement(3722..4531)
                     /gene="dapB"
                     /locus_tag="PKZ49_08435"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HND36626.1"
                     /translation="MLNIAIAGSTGRMGRTLLETIAESPHMRLSAALEQTNSPFLGKD
                     AGELIGAPCDVSINADIAAALSGSDVLIDFTRPAGTLAHLAICRKQGIKMVIGTTGFT
                     ADEKDILKSAANEIAMVFAPNMSVGVNILFKLLEVAAHALRDGYDIEIVEAHHRHKVD
                     APSGTALRMGEVIAQALDRDLSKVAVYGREGVTGERLPETIGFSTIRGGDIVGDHTAI
                     FAGIGDRIEITHKASNRKTFALGALRAAQFLMRQEKGLFDMQDVLGLRQGL"
     gene            complement(4559..5032)
                     /gene="bamE"
                     /locus_tag="PKZ49_08440"
     CDS             complement(4559..5032)
                     /gene="bamE"
                     /locus_tag="PKZ49_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HND36627.1"
                     /translation="MHVKFLLLSIVSLSISCSYLPSLPYKIDIQQGNVVTEDMVEKLR
                     LGMTRSQVRFVLGSPLITDVFHINRWDYVYSLAPSGRLSEKRRLSIFFDGDLLTHIEG
                     DFKLPILPANDLPLPSPNSTDEVPEDDEKKNEDSEELQPKEKKEDKKGTTDMIAP"
     gene            5106..5546
                     /gene="fur"
                     /locus_tag="PKZ49_08445"
     CDS             5106..5546
                     /gene="fur"
                     /locus_tag="PKZ49_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HND36628.1"
                     /translation="MSKEDLKSRDLKTIGLKTTLPRLKILNLFENSAIRHLSAEDIYK
                     ELLNEGEDIGLATVYRVLTQFEQAGLLERHHFEGGKAVFELTSDKHHDHLVCLQCGRV
                     EEFYDADIEKRQIKIAKDRGFSLQEHSLALYADCNKAECPHRKL"
     gene            5586..6032
                     /locus_tag="PKZ49_08450"
     CDS             5586..6032
                     /locus_tag="PKZ49_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36629.1"
                     /translation="MMKRMLWLKVIGLFVLGFYFSTISAQQSGQLIIEAQIQDLEASL
                     LRTTQHQQSVFQQFQMLQEIRRFEVAQDSFTTLSQSPQIGGTLNSPIVDYDDAVRAKQ
                     DRQRKLQQYTLDLETLYQRFQELESEKRMIMEQIDLLNRQKKQKVE"
     gene            complement(6096..7418)
                     /locus_tag="PKZ49_08455"
     CDS             complement(6096..7418)
                     /locus_tag="PKZ49_08455"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HND36630.1"
                     /translation="MAKNVVVIGTQWGDEGKGKIVDWLTDHAAGVVRFQGGHNAGHTL
                     VIDNKKTVLHLIPSGILRSSVMCYIGNGVVLSPRALLEEIDILESAGISVSPRLRISE
                     ACPLILPYHAALDSARETAKGSAKIGTTGRGIGPAYEDKVARRAIRLQDLLCKERFEK
                     KLEEILDYHNFVLKHYFHTTTIDLREVLDEGLAQAERIRPMVADIPELLFQAHKRGAS
                     LLFEGAQGALLDIDHGTYPYVTSSNCIAGAAATGSGVGPQMLHYVLGITKAYTTRVGS
                     GPFPTELDDETGSHLAKRGHEFGSTTGRPRRCGWFDAVAMRRSIQINGVSGLCITKLD
                     VLDGLEKLKVCIGYKNNSTANSVTSSTLNTLPVGAEDLVACEPVYKELPGWSENTKGI
                     TDFKALPSAAQSYLRCLEEVCEVPLDLISTGPDRAETIVFRHPFVDAT"
     gene            complement(7457..>8235)
                     /locus_tag="PKZ49_08460"
     CDS             complement(7457..>8235)
                     /locus_tag="PKZ49_08460"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HND36631.1"
                     /translation="SGLESDIEIQHLMLQCLVKSGVGKIQLDLGHVAIFRCLIKDLAI
                     KPALETDFYTLLRAKDLAALKELLQSTSTLLSKNAQEMLLHLPELYGDRKILIEARKL
                     LPEHPEIIAALDQLEVLAEKLACYVDRITFDLSDLRGYHYHTGIVFAAYTKGNPGPIA
                     LGGRYDEIGKSFGRSRPATGFSMDLRELSRLAHTETSPKGILAPYKPDDKLLQEKVIQ
                     LRASGWVVITLLPGCNEENDATCCDKKLVLKNDTWDVVEI"
BASE COUNT         2328 a         1717 c         1771 g         2419 t
ORIGIN      
        1 aagcgcgtgg cgatacaaaa cgtactgaaa ttattatccc tgatgctgca catggaacca
       61 atcctgcaac cgcggttatg tgtggctata aagtagtgga aattgccact gataaagaag
      121 gtgatgtgga tatggatgca ttaaaagcgg cggtagggcc acaaactgct ggattgatgc
      181 tgactaaccc ttctacactg ggtgtgtttg agaagaatgt atctgaaatg agccgcatcg
      241 ttcatgaagc gggtggcttg ctttactatg atggtgccaa cctcaatgca gtattaggaa
      301 aggttaagcc tggcgatatg ggttttgatg ttatccatat taacttgcat aaaactttct
      361 caactccgca cgggggaggt gggcctggtg ccgcaccggt aggtgtagcg gagcgcttac
      421 tgccttattt accgattccg gtagttgcta agacagaaaa agggaattat cgctggttga
      481 ctgaaaaaga tttacctaaa tcgatcggtc gattgtctgc gcatatgggt aacgcgggtg
      541 tgttactgcg tgcttatatt tatgtgcgtt tgttaggctt tgatggaatg caccggattt
      601 cggaatttgc aacgctgaat gcgaattact taatggcaga attacgtgaa gtgggctttg
      661 aaattgctta cccaacgcga cgcgctagtc atgaattcat tgtgacgctt aaagaaatta
      721 aagatagaac gggtgttact gcaatgaatc tggctaaacg attgttagat aaaggctatc
      781 atgcaccgac aacttatttc cctattcttg ttccagagtg cttgctgatt gaacctgctg
      841 agactgaatc aaaggaaaca ttagatgcgt ttgttgctgc gatgaaagaa attttagttg
      901 aaattaaaga gcaacccgat atggttaaag gtgcacccca caatatgccg ctccgcaagc
      961 ttgatgatgt taaagcggca agagaattgg atttgacttg gaagcctgcg agttagtgtt
     1021 gaattgatcg tcaatttaat gggcacgaag ttagcagtgc ccattaaatt tattctttat
     1081 aacgattatt gagttacgaa aattatgcgt accctcatct gtggttcgat tgcctacgat
     1141 acgattatgg tgtttcaaga tcactttaaa catcatattc ttccggaaaa aattcatgtt
     1201 ctcaatgtgg cattcctagt gcctgaaatg cggcgtgaat ttggcgggtg cgcagctaat
     1261 attgcctata atcttaaaat gttgggtgaa gatcccctca ttatggcaac ggtgggtgat
     1321 gattttcaac cgtacgcgta tcgctgtgag aaagtgggtt ttgataaaac gcatattcgc
     1381 cagattgatg atacttttac tgcacaagct tttatcacca ctgatttaga tgataaccag
     1441 attactgctt ttcatcctgg cgcaatgaat ttctcgcatc ataactcggt gaaagaggct
     1501 aagcatgtta gccttggtat cgttgcaccg gatggacgag atggaatgat tgctcatgca
     1561 cgtgagtttc aagaatgtgg aatccctttc atttttgatc cagggcaagg tctcccgatg
     1621 ttcagtggcg atgagttggt tgatttcatt agcaaagcag actatgtgac tgttaatgat
     1681 tatgaagctg aattgttatt ggagcgaacg gggtattcct tgggaaaatt agctagtctg
     1741 gttaaagcgt taattgttac taaaggggct caaggttccg tcatctacac gggcgggcat
     1801 catatcgaga ttccaagtgt ccagcccaaa gcgattgtag atccaacggg ttgtggtgat
     1861 gcgtatcggg ctggtttgct ttatggaatt gctcatggtt tggattggta tgcaacagga
     1921 aaactggctt cattactagg ggcactcaaa attgcttatc ggggtggtca aaatcatgaa
     1981 tttgatcgtt ctgctattga tcagcactat tttgaagctt atggaaatcg tattttctag
     2041 acaataaccg ctatttttta ggttattgta cgaatcctat taaagcttat tgtgggtggg
     2101 ggctttaatt catggctgaa taacaatttt attggttcta tcccattcca tgacgagcca
     2161 cgttaagact cataagtttc ttcaattttt ccgctttctc tgtttaatca ttcactttgt
     2221 atcaggatta ctacagtcgt ttatttatcc ccatgtaaat cgccgtacgc agcgttatat
     2281 ggctaagaaa tgggcgcaac gctttctgcg tattcttaat atcaaactgc actatcatgg
     2341 atcacttcct agcgatgatc aggcacgcgt gatactggct gccaaccata tttcttggct
     2401 tgatgtcatt gcgattcttg caatctatcc cgtccgcttt gttgctaagt cagaaattct
     2461 agactggccg ataattggaa aattgtctgc taacgcaggt acaatttttg tggcacgcga
     2521 aaaacgcaat gacactttaa gagttaatca atttctcagt gaaattttaa tagcgggtga
     2581 tcgtattgct atttttccag aaggtacgac gagcgatggg acgcatctgc ttcattttca
     2641 tgcatcattg ctgcagtttg cggtggcttc gaagggattg gtttatccag tagcgattca
     2701 ttatcgagat gccatgaatc agcctactac gctaacagcg tatgtcgatg tttcgatcac
     2761 ccaatcatta cagttgatac tgcaacaacc gagttgccat gttgaattaa attttctgac
     2821 cccattgcag ggcgaggaaa agaatcgacg agaactagct cggcttaccg agcatgcgat
     2881 ttcacaccag ctcgctttac cggttgtacg caagggatct gaaaaatctt ccgatcttcc
     2941 aagcgaatcg cagtgagctc agcgccccac aagcatccgg tgtccaatgc aatgagatta
     3001 tgtcttagct gtagccttat ggcagaccaa tgcccaaaaa taatggttga tttttggctt
     3061 tgtctactcg gcacatcgaa ccaagggaaa tagccttcgg gaatcgtttc taggggtcct
     3121 ttataagaaa aatccatttt cccttctttc gtgcagatgc gtagtcgcgt taatgcatta
     3181 acgattaagc gtaaccgatc gtaggatttt aggtcatcat gccattgatc gggttgattg
     3241 ccatataaat gcagtaatag gtcggtgtaa tcgttgcttt gtaatgttgc ttcgacttct
     3301 tgggccagtt ctagggcaaa cgaagcaggc catgatggta ataatcctgc atggaccaga
     3361 acataatcat cttcgacatg aagcattttt tggtgccgga gccaataaag taattgatta
     3421 cgatctggcg cattgagaat tgcttgtaag gtatcgccac tccgttgcga ggtgatttct
     3481 tccgcaacca gtaataaatg taaatcatga ttgcctaata cagtcgtagc tgaatcgccc
     3541 aattgactga tgaagcgtaa taaactgagc gaatcggggc cgcgattaac gatatcgcct
     3601 acaaaccata gcctgtcgcg cgaaggtgag aatgcgataa actcaagcaa gtgttggagc
     3661 tgtttgaagc aaccctgtaa atcaccaatt gcataagttg ccataaaaat cagctaccga
     3721 tttataggcc ttgtcgcaac ccgagaacat cctgcatgtc gaataaaccc ttttcttgcc
     3781 gcattaaaaa ttgagctgcg cgcagtgcac ccagtgcaaa tgttttacgg ttactggctt
     3841 tatgggtaat ttcaatacga tcaccgattc cggcgaaaat tgcagtatga tcgccaacga
     3901 tgtctccgcc gcgtatcgtg gaaaacccaa tagtttctgg taatcgttcg cctgtcacac
     3961 cttctcttcc atagacagca acttttgata aatcgcgatc taaggcttgg gcgattactt
     4021 ctcccatccg cagcgctgtt ccagaaggcg catcgacttt atgacgatga tgcgcttcta
     4081 caatttcgat atcgtagcca tcccttaatg cgtgcgcggc aacttcgagt aacttgaata
     4141 gaatgtttac ccctacactc atattaggcg caaaaaccat cgcaatttca ttagcagccg
     4201 atttaagtat atctttctcg tcggctgtaa accctgtggt accaatgacc attttgatac
     4261 cttgcttgcg acaaatagct agatgcgcta gcgtacctgc tggacgcgtg aaatcgatta
     4321 aaacatcgct gccggataac gctgctgcga tatctgcatt aatagaaaca tcgcaaggag
     4381 ctccaattaa ttcaccagca tcttttccga gaaaggggct attcgtttgc tctaacgctg
     4441 cggataaacg catatgaggt gattccgcta tggtttccag taatgttcgc cccatacgac
     4501 cagtactacc tgctattgct atatttaaca tagattttga ttcattatga gaatagtttt
     4561 aaggagctat catatctgtt gtaccttttt tatcttcctt cttttctttt ggctgtaatt
     4621 cttcagaatc ctcatttttc ttttcatcat cttcaggaac ttcatcagtg gaattaggtg
     4681 aaggcaatgg aagatcatta gccggtaaaa ttgggagctt aaaatcacct tcaatatgcg
     4741 tcaataaatc accatcgaaa aaaatggata aacggcgttt ttctgatagc ctcccgctag
     4801 gggccaagct atacacgtaa tcccatcgat taatatgaaa aacatcagta atgagcggtg
     4861 accccagtac aaaacgtact tgggatcgag tcatacccag tcgcagcttt tctaccatat
     4921 cctcagttac gacattacct tgttgaatgt caattttgta gggaagtgat ggaagataag
     4981 agcagctaat cgatagcgat acaatactaa gcaacaaaaa ttttacatgc atactcaaac
     5041 gagagttttc aaagctaacc aagcgctata tgatacagca aataactttt aggactgatt
     5101 aaaccatgag taaagaagat ctcaaaagta gagacctcaa aacaataggg cttaagacta
     5161 ccttaccccg tttgaaaata ttaaatttgt ttgagaatag tgctatccgt cacttgtcag
     5221 ctgaagatat ttataaagaa ttacttaacg aaggtgaaga tattggtctt gcaacggttt
     5281 accgcgttct tactcagttt gagcaagcgg gtttattgga aaggcaccat tttgaaggcg
     5341 gtaaagcagt tttcgaatta accagcgata agcaccacga tcatttggtt tgcctgcagt
     5401 gtggccgtgt cgaggaattt tacgatgctg atattgaaaa gcgccaaatc aagatcgcca
     5461 aagatcgtgg atttagcttg caagagcatt cacttgcact gtacgcagat tgcaacaaag
     5521 ctgaatgtcc ccaccgcaaa ttataaaacc agtactatca tcgatcattc agtgcaaaaa
     5581 aatttatgat gaagcgtatg ttatggctca aagtgattgg attgttcgtt cttggttttt
     5641 atttttcaac aatctcagcg caacaatcag gtcagctgat cattgaagca caaattcaag
     5701 atttagaggc gagcttatta cgaacgactc aacatcaaca aagtgttttt cagcaatttc
     5761 agatgttgca agagattcgc cggtttgaag tagcgcaaga tagctttacc acattatccc
     5821 aatcgcccca aattggggga acactgaact caccgatcgt tgattatgat gatgcggttc
     5881 gcgctaaaca agatcgacag aggaaattac agcaatatac gcttgatctc gagacgcttt
     5941 atcaacgatt ccaagaacta gaaagtgaga aaaggatgat tatggaacag attgatttgc
     6001 tgaatcgcca gaaaaagcaa aaagtagaat gagggggagt gattggtatt gttgaaagta
     6061 catacagcaa agtaagattt tctaggcaag ttatcttagg ttgcatctac aaaagggtgt
     6121 ctaaatacga ttgtttcggc gcgatcaggc ccagtagaaa taagatctaa tggaacttca
     6181 catacttctt ccaagcaacg taagtaactt tgagctgcgc ttggtaacgc tttaaaatca
     6241 gtaatacctt tagtattttc actccatccg ggtagttctt tataaaccgg ctcgcaggcc
     6301 acaagatctt cagcacccac tggcaaagta ttcaaagtgc tacttgtgac tgaattagct
     6361 gtggaattat ttttataccc aatacaaact tttaattttt ctaaaccatc taaaacatct
     6421 aatttagtaa tacataaccc ggatacccca ttgatttgta tcgatcgccg cattgcgaca
     6481 gcatcaaacc atccacagcg acgaggacgc cctgtggtag atccaaactc atgtccccgt
     6541 tttgctaaat gtgaacctgt ttcatcgtca agttcggtag gaaaggggcc agaacccacg
     6601 cgagtcgtat aagctttggt tatccccagt acataatgta acatttgtgg accaacaccg
     6661 ctcccagtag cagcagcgcc tgcaatacag ttgcttgagg taacataggg ataagtgcca
     6721 tggtcaatat cgagtaatgc tccttgagca ccttcaaata gtaaactagc tccgcgctta
     6781 tgggcctgga acagtaactc gggtatatcg gctaccatcg gtcgaattcg ttctgcttga
     6841 gcaagtcctt catcaagaac ttctctcaaa tctatcgtcg tggtatgaaa gtaatgtttg
     6901 agaacgaaat tatggtaatc aagaatttct tcgagtttct tttcaaatcg ttctttacaa
     6961 agtaagtctt gcagacggat agcccgtcgc gccactttat cctcataggc ggggccaatg
     7021 ccacgtccgg ttgtgccaat tttagctgat cctttcgcgg tttcacgtgc gctgtctagg
     7081 gcggcatggt acggtaggat taatggacaa gcttcactaa ttcttaatcg aggtgatact
     7141 gagatacctg cactttcaag gatatcgatt tcttctaata aggcgcgtgg tgacagaaca
     7201 actccgttac caatgtagca catgaccgaa ctacgcaata ttccagatgg gataagatgt
     7261 aatacagttt ttttattatc gatgactaag gtatgaccag cattatgtcc accttgaaaa
     7321 cgaacaacac ccgctgcgtg atcggttaac caatcaacaa ttttcccttt tccttcatca
     7381 ccccattggg taccaattac cactacattt ttagccatag ctttatttct ctttacctga
     7441 attacttaag gcagtatcaa atttctacaa catcccaagt gtcattttta agaacgagct
     7501 tcttatcaca acaagtggca tcattttctt cattacaacc cggcaaaagt gtgatgacaa
     7561 cccagcccga tgcccgcagt tgtatcacct tttcttgtag taacttatca tcaggcttat
     7621 agggcgctaa aattccttta gggctcgttt cagtatgtgc tagccgagat aattcacgta
     7681 aatccatact aaaacctgtt gctggccttg atcttccaaa agatttacca atctcatcat
     7741 atctgccacc caacgcaata ggcccaggat ttcctttcgt ataggcagca aaaacaatac
     7801 cagtatgata gtggtagccc cttaaatcag atagatcaaa cgtaatccga tccacgtaac
     7861 atgctaattt ttctgcaagc acttcgagct ggtcaagtgc agcgatgatt tcagggtgct
     7921 ctggtagcag tttacgtgct tcaatgagta tttttctgtc gccatataat tcaggtaaat
     7981 gcaataacat ttcctgagcg tttttactga gtaaggtgct agtcgattga agtaattcct
     8041 taagtgctgc taaatcctta gcgcgtagca gggtatagaa atctgtttcc agtgcaggtt
     8101 taattgctaa gtctttaatt aagcacctga aaatagcaac atgtcccagg tctaattgta
     8161 tttttccgac ccctgacttt acaagacatt gcaacattaa atgttgaatc tcgatatcgc
     8221 tttctaaacc tgaat
//
LOCUS       DAOQKQ010000115         8234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_4209_length_8234_cov_4.787382, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000115 DAOQKQ010000000
VERSION     DAOQKQ010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 8234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8234
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4209_length_8234_cov_4.787382"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="PKZ49_08465"
     CDS             <1..259
                     /locus_tag="PKZ49_08465"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HND36632.1"
                     /translation="TESVYEIGGVVIDKKTGIVLKGAEVTLIETGKLAITDQTGQYYF
                     TGLNKGAYHLRAVKSNYSTATQEIGVPSNSSTAFDIKLSPT"
     gene            272..1426
                     /locus_tag="PKZ49_08470"
     CDS             272..1426
                     /locus_tag="PKZ49_08470"
                     /inference="COORDINATES: protein motif:HMM:NF016845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HND36633.1"
                     /translation="MGSYLSPGVYVEEVPPLARPIAGVGTSTAGFIGVISDAATLKMP
                     DKPDGSGKYTLADAGKSILVTSWNEFVTNFGEIQAGNSDLAHAVFGFFHNGGTRCWVI
                     HVTALDDLSSELGEFAAIDEIAIVAVPGATTKPIHAALLDHCLQLGDRVAILDGVKDA
                     DPPTADNISPEGRSEKGSYGAIYFPWIKVSNPLKKNETITVPPSGHIAGIYARNDARR
                     GVHKAPANEVVLGALGLEKRISKAIQNDLNPEGVNVIREFGREIKVWGGRTRADSGHS
                     EYQYISTRRYMNFLRESIDEGTQWVVFEPNSHSLWQRISRTLTDFLTNQWRQGALFGE
                     TPKQAFFVKCDADTNPPDVRELGQVVTEIGVAIVKPAEFVIFRIQQTTGG"
     gene            1464..1889
                     /locus_tag="PKZ49_08475"
     CDS             1464..1889
                     /locus_tag="PKZ49_08475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HND36634.1"
                     /translation="MARVDPYKNFRFLLEIDGITQAGFSDCSGFGSNVEVVEYREGGE
                     APTVRKLPGKTSYPDITLKWGITDSRDLYDWHLSAINGNLVRKNGSIILQDDLGQEKV
                     RWNFFTAWPSKYDGPDLSAKGNDVAIDTLTISCERLVRA"
     gene            1886..2257
                     /locus_tag="PKZ49_08480"
     CDS             1886..2257
                     /locus_tag="PKZ49_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36635.1"
                     /translation="MSEFATEFEFVLPKGYIDSVGTVHREGIMRLATAADEILPLKDP
                     RVQNNSAYLVVILLSRVITRLGELDAINPKVIEGLFAADLTYLQEFYNRINSAQTRKI
                     AVMCPQCQHHFNVEPELAGGS"
     gene            2471..>2951
                     /locus_tag="PKZ49_08485"
     CDS             2471..>2951
                     /locus_tag="PKZ49_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36636.1"
                     /translation="MIEELNQKQHNARFGVLHDRSTWRGLSEISRTCLLEAMWQRYNF
                     SLTIQCNKNARRNGTMMNFPPLELALLRSEQPLSLAPSVTVNLWQHYQIVKRIMSGNP
                     EIQSVTSVAIAKQRLNALVTSKGKPIYQGHKQQLTVQSENSSAFALSAHGALRTGVLP
                     "
     assembly_gap    2952..3051
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3142..3705
                     /locus_tag="PKZ49_08490"
     CDS             3142..3705
                     /locus_tag="PKZ49_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36637.1"
                     /translation="MSMARQAPLKHNQHDTVLSGPAAELQQNPLNHLEKSPALVLSTH
                     HTPRSEVSPAQYFKRSGKLPSPTNQQAHQQHSQHIAVRRELAEGKEHSSPNSLADILF
                     DRAMQSQPRLPSDFSLRMLPPESAEVAVQANAPDTKSMQLGTKSAVNSKDPIQLNGSD
                     ITRVADQVAQVLNRRKKFERERRGEFC"
     gene            3705..4331
                     /locus_tag="PKZ49_08495"
     CDS             3705..4331
                     /locus_tag="PKZ49_08495"
                     /inference="COORDINATES: protein motif:HMM:NF039279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HND36638.1"
                     /translation="MALAKAVIINLDERTPLPIPVMFNPPEYTLSKTNQFAEIKIPGL
                     PSSILQFVNGDAQSLSMELFFDTTDSSLDVRSRTIAIANLTEPTAQTKAPPRLLLLWG
                     SLAFSCFLISVKQHFEYFNSFGLPLRARLTVEFKGNTTAESMVAGAPAALVEQAARYI
                     VKSSDTLQGIAAAQLNDPSKWREIAIANNIDNPRALANGQRLTMPKSV"
     gene            4334..5410
                     /locus_tag="PKZ49_08500"
     CDS             4334..5410
                     /locus_tag="PKZ49_08500"
                     /inference="COORDINATES: protein motif:HMM:NF017739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HND36639.1"
                     /translation="MNLSVDFSMLGQEVTVPIFEIRVNGTPLAVPYHKAITGVSVDLV
                     TNEPCAFALQVNDPKLELVDAKQGIFAEGKELEIFMGYTRATQSMIAGEITSIGANLD
                     EGGGLTLNVHGFDKLHTGTRGTGYREFREKKRDSDIVREIATDMQLTADVDVTEEQGK
                     QQIQNHVSNLEYIQYLAKKNGFFFWVEDNKLMFKRSRDRKEIRIARGKNLISFSTQLS
                     TSGQTSAVEVRGWDAAQKRAFSARAEATQSQAYIAGLSATGQTQIKGIGSNRSERIIY
                     ADGEVGSIAEAQRLADAELAKQQRALFSIQGSTIGNPDIRVGNILILEKMGRFSDVKY
                     VVETAFHEINQSGYRTSFTASQRI"
     gene            5407..5925
                     /locus_tag="PKZ49_08505"
     CDS             5407..5925
                     /locus_tag="PKZ49_08505"
                     /inference="COORDINATES: protein motif:HMM:NF016595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HND36640.1"
                     /translation="MNGAIPGVYIGIVKSNKLDAEGRIEITLEGVSGNAKSYPARIAT
                     LMAGNGYGTLLLPEKDDQVLVAFINGAISEPVVIGGLWSVQDKSPETNASGENNIKLI
                     RTRSGNEIRIIDEGGKELIEIQSPNGKIMIKGSEITLDGSVHITGTLDVGPDSGSQTH
                     IDGNEITGRTSS"
     gene            5922..6386
                     /locus_tag="PKZ49_08510"
     CDS             5922..6386
                     /locus_tag="PKZ49_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36641.1"
                     /translation="MTDLILKGPLNLMGSLKLAADGGKVMVNEAEILVEVGRNGKSQG
                     MGIPVIQPPPPAPKPIDDGSEVRIFNSFNSRVTIKVNGADIRVVALGLCIQGNLPGGT
                     WPGMVLPSMNNRSLVTINQMPVTINQIPINVKGDQGITLVNGGTINFTDSGQ"
     gene            6395..7183
                     /locus_tag="PKZ49_08515"
     CDS             6395..7183
                     /locus_tag="PKZ49_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36642.1"
                     /translation="MAFIKLSIVTPEPEPKIPITGAPAKVIFQGSATIPLGTPQLFYR
                     WYSSSVVRLSSSDPPIAGGYSMNPAPITNPDVPFEYELGLGSHVITFAVSDQPGESNQ
                     DFENIQHAGITGGNTGDSTKCVIHVYAAKIVGLSSGQTISKTNLRLSAEAPAEWDAKY
                     LDAKTNQYIFPYQQINQLAYHWKLEPLGNPPGRSTHESGQLNHGDKLSFSQDTGKPIV
                     TYQPDLSSASENDFEGSYRITLSVLKKESGAPVDSARVEVFLTH"
     gene            7225..7635
                     /locus_tag="PKZ49_08520"
     CDS             7225..7635
                     /locus_tag="PKZ49_08520"
                     /inference="COORDINATES: protein motif:HMM:NF016829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HND36643.1"
                     /translation="MNENSYSSDEILGRGVAFPLQLAGGIVGTNAYENQVKQSISLIL
                     QTTPGERMMRPDFGAGMETLAFEPMSAITTALVQHRIQEALARFEPRINVLNVTVNTE
                     QSEGKLMVDIQYRVTRTGSVFNLVYPFYIERGVL"
     gene            7635..>8234
                     /locus_tag="PKZ49_08525"
     CDS             7635..>8234
                     /locus_tag="PKZ49_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36644.1"
                     /translation="MLSKAPLIDTRSTAEIARQIRQLLNIYLRNAPYNWESADGGEIG
                     RALTEISAYYCGLIVDRINRAPENNLLAFLDLLGNSLVLPVPAQVPVTFFLDTRATEG
                     LNLPAGTRMLAEPERNSNDPVPFETSRDLWLTTLELTKLENEPESKEYSNLIKNRPNL
                     TKNLSETPLAIFKDKTDSYIFEFTLPAESKLPVNHPVTLF"
BASE COUNT         2306 a         1847 c         1973 g         2008 t
ORIGIN      
        1 aaccgaatcg gtatatgaaa tcgggggggt agtaattgac aaaaagaccg gaatcgtgct
       61 caaaggggcc gaggttactt tgatcgaaac tgggaagctt gctatcacag atcaaacagg
      121 gcaatattat tttactggat tgaataaagg ggcttatcat ttacgtgcag taaaatcaaa
      181 ctattcgaca gctactcaag agattggagt gcctagcaac tcatccaccg ccttcgatat
      241 taagctatca cctacttaga ggagaatctt gatgggatca tatctatcac ccggcgttta
      301 tgttgaagaa gtaccgccat tggctcgtcc gatagcaggt gttgggacaa gtacggccgg
      361 gtttatcggt gttatttcag atgccgcgac cttgaaaatg cctgataaac cggatggcag
      421 cggaaaatat acactcgctg atgcgggcaa aagcatactt gtgacaagtt ggaacgaatt
      481 tgtaacaaac tttggtgaga ttcaggctgg taactcggat cttgcacatg ctgtatttgg
      541 atttttccat aatggaggaa cccgatgttg ggtaatacat gttactgctt tagatgatct
      601 cagcagtgaa cttggagagt ttgcagcgat tgatgaaatt gcgatcgtgg cggttccagg
      661 tgcaaccaca aagcctattc atgcagcttt gttggatcat tgcttgcagt taggtgatcg
      721 tgtcgctatc cttgatggcg tcaaagatgc agaccctcct actgcggata atatcagccc
      781 tgagggcaga agcgaaaaag gaagctacgg tgctatttac tttccatgga ttaaggtttc
      841 taatccattg aaaaaaaacg aaaccattac tgtgccgcct agcggccata ttgctggaat
      901 ttatgcacgt aatgatgcgc gacgtggagt tcataaagca cctgccaatg aagtcgtact
      961 tggcgcactc ggactggaga aaagaatcag taaagccata caaaacgatt tgaatcccga
     1021 aggagtcaat gtaatccgag aatttggtcg cgaaataaag gtttggggcg ggcgaacacg
     1081 tgccgacagt ggacattctg aataccagta catcagcaca cgccgttaca tgaatttttt
     1141 gcgtgaatca atcgacgagg gaacgcaatg ggttgtcttt gagcccaatt ctcattcatt
     1201 atggcagcgc atttcccgta cattaacaga ttttcttacc aatcaatggc gccaaggtgc
     1261 tctatttggg gaaacgccaa agcaagcatt ctttgtcaaa tgtgatgcag atactaatcc
     1321 acccgatgtt cgtgaactgg gtcaagttgt cacagaaatc ggggtagcca ttgtcaaacc
     1381 ggcggaattt gtaatcttcc ggattcaaca aacgacaggc ggttaatcaa accataacat
     1441 gttaataaag aaaggaagct gaaatggcca gagtagatcc ttacaaaaat tttagatttt
     1501 tattggaaat tgatggtatt acacaagccg gtttttccga ttgcagtggc ttcggttcga
     1561 atgtggaagt tgttgaatat cgtgagggag gggaagctcc gaccgtgcgc aaactgccgg
     1621 gcaagaccag ttatcccgat atcactctta aatggggtat aacggattca cgcgatttgt
     1681 atgactggca cctctccgca attaacggaa atctcgtgag aaaaaatgga tcaatcatct
     1741 tgcaagatga cttagggcag gagaaagtgc gttggaattt ctttactgcc tggcccagca
     1801 aatacgatgg tcccgattta agcgctaaag gcaatgatgt tgccattgat acgttaacca
     1861 ttagctgcga aagattggta agggcatgag cgaatttgcc accgaatttg agtttgtgct
     1921 gccaaaaggc tatatcgaca gtgttggcac tgtgcaccga gaaggaatca tgcgactggc
     1981 aacagcagcc gatgaaattc ttccattgaa agatccgcgt gttcagaaca attcagccta
     2041 tcttgtagtt attctgctat ctcgtgtgat tactcgtttg ggcgagcttg atgcgatcaa
     2101 ccccaaagtg attgaaggtt tgttcgcagc ggatttaacg tatttgcagg aattttataa
     2161 ccggatcaat agtgcacaaa cccgcaagat tgcagtcatg tgcccgcaat gccagcatca
     2221 ctttaatgtg gagccagaac tcgcgggggg gtcataggct accctctcgc ccggctgtac
     2281 gaggaggtag cctttattgc ctatcatttt cactggccgc cggaacagct actgaatctg
     2341 gagcacgcag accggcggcg ttgggttgag gaaatttcga gaatcaacaa gacggttatg
     2401 aaacctgcag aatgagtgac acaaaccggt ttcctcaaaa gcttcgtaat tgattgtggg
     2461 agcactgaga ttgatagaag aattaaacca gaaacaacat aatgcacgat tcggtgtact
     2521 gcatgatcgg tcaacttggc gcggactaag tgagatcagc cgcacatgct tgttggaagc
     2581 catgtggcag cggtataatt tttccctcac aattcagtgt aataaaaacg ctaggcgtaa
     2641 cgggacaatg atgaattttc cgcctctgga acttgcattg cttcgatcgg agcaaccact
     2701 ctcattagca ccctctgtaa cggttaatct gtggcagcac taccaaatcg tcaaacgcat
     2761 aatgtcgggt aatcctgaaa tacagtccgt tactagcgtt gctatcgcca agcaaagatt
     2821 aaatgcgcta gtgacatcca aaggtaagcc aatttatcag ggacacaagc agcagcttac
     2881 cgttcaatcc gaaaactcat cggcattcgc tctatctgcg catggtgcgt tgcgtaccgg
     2941 ggtattaccg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntcatcggca
     3061 ttcgctctat ctgcgcatgg tgcgttgcgt accggggtat taccggtaca ttatcccagg
     3121 cacagcaaca agcagttggc tatgtctatg gctcgacaag cccctctaaa acataatcaa
     3181 catgataccg ttctttccgg gcctgctgcg gaattgcaac agaacccgct taaccatctt
     3241 gagaaatcgc cagcactcgt tctttccaca caccacaccc ccagatccga ggtatcaccg
     3301 gcacagtatt tcaagcgaag cggtaagctt ccctcgccga cgaatcaaca agctcaccag
     3361 caacatagtc aacacattgc agttcgccgc gagcttgccg aagggaaaga gcacagctcc
     3421 cccaatagct tggctgatat cctatttgat cgcgcaatgc agtcgcagcc acgactgccg
     3481 tccgatttta gcttacgcat gctaccgcca gaatcagcag aagttgctgt tcaagcaaat
     3541 gcaccagaca caaaatcaat gcaactcggt acaaaatctg cagttaatag taaagatccc
     3601 attcaactga atggcagcga cattacacga gtggctgatc aagttgcaca agtattgaat
     3661 cgacgcaaga aattcgagcg ggaacgtaga ggggaatttt gctaatggca ctcgcaaaag
     3721 ctgtaattat taatctcgat gagcggactc ctttaccgat tcctgtgatg ttcaatccgc
     3781 cggaatatac tctctccaaa accaatcaat ttgctgagat taaaatcccc ggattgccgt
     3841 cttcaattct gcagtttgtc aatggcgatg cacaatcatt gtcaatggaa ctcttttttg
     3901 acaccaccga ttccagcttg gatgtgagat cgcgcacgat agccattgca aacttaactg
     3961 aacccactgc acaaacaaaa gcgccgcctc gcctgctatt gctctggggt tcactggctt
     4021 tctcatgctt tctaataagc gtcaaacaac attttgaata ttttaattct ttcggtttac
     4081 cattgcgggc tcgccttacc gttgaattca aaggtaatac aacggcggag tcgatggttg
     4141 ctggcgcacc agctgcgctg gtcgagcaag ctgcgcgcta tattgtaaaa agtagcgata
     4201 ctctacaagg aatcgccgct gctcagctta acgacccgag caaatggcgc gaaattgcca
     4261 tcgcaaacaa tatcgataat ccgcgcgcac tggcaaatgg ccagcggctc acaatgccaa
     4321 agtcagtgta accatgaatt tatcggttga tttcagcatg ctgggccagg aagtaacggt
     4381 gccgattttt gagattcgag ttaacggcac gccattagct gtgccctatc acaaagcaat
     4441 caccggtgtg agcgtggatc tggttaccaa tgaaccatgc gcctttgctt tacaagtaaa
     4501 cgatcccaag cttgaacttg tggacgctaa acagggcatc tttgccgaag gaaaagaact
     4561 ggaaattttt atgggatata cccgggccac gcaatccatg atcgcgggtg agattacttc
     4621 catcggagca aatttagacg aaggcggcgg tctcacactc aatgtacatg gattcgacaa
     4681 attacacaca ggaacccgcg gtaccgggta tcgtgaattc cgtgaaaaga aaagagatag
     4741 cgacatcgtg cgggaaattg caacggacat gcaattaacg gcagacgtcg atgtcactga
     4801 ggaacaggga aaacagcaaa ttcaaaatca cgtatccaat ctcgaataca ttcaatactt
     4861 ggcgaagaaa aatggcttct tcttttgggt ggaagacaac aaactaatgt tcaagcgctc
     4921 tcgagataga aaagaaatcc gcatcgcgcg tggcaaaaat ctgatttcgt tttccacgca
     4981 actgagcaca tccggacaaa ccagtgcagt cgaagtgcgc ggttgggatg ccgcgcaaaa
     5041 acgcgcattc tccgcccgcg cggaagccac acaatcacaa gcttacatcg ccggattgtc
     5101 ggcaaccggg caaacacaaa ttaaaggcat cggcagcaat cgctctgaac gcatcattta
     5161 tgccgatgga gaagttggat ccattgccga agcgcagaga ttagccgatg ccgagttggc
     5221 aaagcaacaa cgcgcattgt tttcgattca aggtagtacc atcggtaatc cggatatccg
     5281 ggtgggtaat attctgatac tggaaaaaat ggggcgcttt agcgatgtta agtatgtggt
     5341 cgagaccgca tttcatgaaa tcaaccaatc cggttaccgt acttccttta ccgcaagtca
     5401 gcgcatatga acggagccat accgggtgtt tatataggaa tcgtgaaaag caacaagctt
     5461 gacgctgagg gtcgaattga aattacgctc gagggcgttt ccggcaatgc gaagtcctac
     5521 cccgcccgga ttgcgacgct gatggctggc aacggttacg gcactttatt gctgcctgag
     5581 aaagacgatc aggtattggt ggcctttatc aatggcgcca tcagcgaacc tgtcgtaatc
     5641 ggcggcttgt ggagcgttca ggacaagtct ccggagacca atgccagcgg tgagaataat
     5701 atcaaattga ttcgaacgcg aagcggtaac gagatcagaa ttatcgacga gggtggaaaa
     5761 gagctgattg aaattcagtc acccaacgga aaaattatga ttaagggcag tgagattaca
     5821 ctcgatggga gtgttcacat cactggtacg ctggatgtgg gtccggattc aggatcccaa
     5881 acccatattg atgggaatga gattacagga aggacatcat catgaccgat ttaattctaa
     5941 aggggccatt gaatcttatg ggcagcctaa aactcgcggc tgatggcggc aaggtaatgg
     6001 taaatgaagc cgaaatatta gttgaggttg gccggaatgg taagagtcag gggatgggaa
     6061 ttccggtaat tcagccgccg ccacctgccc caaagcctat cgatgatggt tccgaagtaa
     6121 ggattttcaa ctcttttaat tccagggtaa ctatcaaagt aaacggtgcg gatattcgag
     6181 tagtcgcact gggtctctgt atacaaggaa atttgcctgg tgggacatgg ccaggcatgg
     6241 tgttgccgag tatgaataac agatcactcg tgactatcaa ccagatgccc gtaactatca
     6301 accagattcc catcaacgtg aaaggtgatc agggaatcac cctggtcaac ggcgggacta
     6361 ttaacttcac cgacagtggc cagtaggagt gcatatggcg tttatcaaat tgagcattgt
     6421 cacaccagaa ccagaaccta aaataccgat tacgggggca ccggctaagg taatttttca
     6481 aggatcggca acaataccat taggcacccc tcaattgttt taccgctggt attcaagcag
     6541 cgttgtcagg ctttcttcct cagacccacc tatagctgga ggttactcga tgaatccagc
     6601 tccgataacc aatcctgatg ttccttttga atatgaactg ggcttgggca gtcacgtaat
     6661 tacgtttgct gtgagtgatc aaccgggtga atcgaatcag gatttcgaga atatccaaca
     6721 tgctgggata accggtggca acacaggtga ttccaccaag tgtgttattc atgtttatgc
     6781 ggccaagatt gtaggcctta gcagcggcca gacaatctca aaaaccaatt tgagactaag
     6841 cgctgaagct cctgcagaat gggatgcgaa gtaccttgat gcgaaaacca atcaatacat
     6901 ttttccttat caacagatca atcaacttgc ataccactgg aagctggaac cacttggaaa
     6961 cccaccgggg aggtctactc atgaatccgg tcagcttaat catggtgata agcttagctt
     7021 ttcccaagat actggaaaac ctatcgttac ttatcagccg gatctttctt cagcgtcaga
     7081 aaacgacttc gaagggtcgt accggattac cttgagcgta ttgaagaaag aatcgggtgc
     7141 cccggttgat agtgctcgag ttgaagtttt tcttacccat taaaagtggc aatgtgctag
     7201 ttattaacaa tatggaggca acatatgaat gagaatagtt acagcagcga tgaaattctc
     7261 ggacgcggcg tcgcctttcc gttgcaactc gcaggtggta tcgttggcac gaatgcttat
     7321 gaaaatcaag ttaagcaatc gatttcgcta atactgcaaa cgacaccggg tgagcggatg
     7381 atgcgcccgg attttggtgc ggggatggaa acacttgctt ttgaacccat gagcgccatt
     7441 acgactgcgt tggtgcaaca tcgcattcaa gaagccttgg ctcgctttga gcctcgcatc
     7501 aatgtgttaa acgttacggt aaacactgaa caaagcgaag gcaaattgat ggtcgacatt
     7561 caataccgcg taacgcgtac gggatcggta ttcaatttgg tgtatccgtt ttatattgaa
     7621 cgcggagttt tgtgatgctt tccaaagcgc cattgattga tacgcggagc actgctgaaa
     7681 ttgccaggca aattcggcaa ttattgaaca tttatctgag aaatgccccg tacaattggg
     7741 aatctgccga tggcggcgag atcggccggg cactaacgga aatttctgca tattactgcg
     7801 gattgattgt cgatcgaatt aaccgcgcgc ctgagaataa tctattggct tttctcgatt
     7861 tgctaggaaa ttctctggtg ctgccggtac ctgcgcaagt acccgttact ttttttttgg
     7921 atacgagggc aacggaaggt ctcaacctcc cagccggaac ccgcatgctg gccgagccgg
     7981 aaagaaacag caatgatcct gttccattcg aaacatcccg cgacctctgg ctaacaacgc
     8041 tagaactgac gaaattggag aatgaaccag agtcaaaaga gtattccaat ctgattaaaa
     8101 atcgacccaa tctgactaaa aatctatcgg aaacgccatt agctattttt aaagataaaa
     8161 cagactctta tatttttgag tttaccttgc ctgcggaaag caagcttccg gttaatcacc
     8221 ccgtaacact attt
//
LOCUS       DAOQKQ010000116         8133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_4290_length_8133_cov_5.585789, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000116 DAOQKQ010000000
VERSION     DAOQKQ010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 8133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8133
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4290_length_8133_cov_5.585789"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            317..1012
                     /locus_tag="PKZ49_08530"
     CDS             317..1012
                     /locus_tag="PKZ49_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HND36645.1"
                     /translation="MKPEDVAQYLQENPQFFETYADMLAEIKIPQPYDGRAISISERQ
                     ISTLREKNKLLQEKLHELINIGENNDTIGEKMHRLAVSLLEFNSVEELLHGLNYNLCE
                     DFSIPHVVMRLWYLNDIKSNQKRPEFSAVSKTVRISVESMRKPYCGPQVSDDIKPWFG
                     QDAKYLQSFSIIPLRRKRTIGLLVMGSPDAERFYPDMGTLYLERLGELVSSALARLER
                     IDLEQIIDEPPKI"
     gene            993..1916
                     /gene="xerC"
                     /locus_tag="PKZ49_08535"
     CDS             993..1916
                     /gene="xerC"
                     /locus_tag="PKZ49_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HND36646.1"
                     /translation="MSPPKFEEKPVSISAYLAYLRQVRRLAKLTYKSYERDLAVLNQF
                     SMSIPFHELEAHDIRHFIARLHGSGLSGKSLARTLSAWRTFYQYLIRFRGYQNNPCQG
                     IKVPKSPKHLPHTLSPDEAAKLMDFESTDSLSIRDRAMFELCYSSGLRLQELVQLKPH
                     DIDFTEAQVRVVGKGEKTRIVPVGDYALAALSAWLKQREALPKLDSDALFVSSRGNRI
                     HPRTVQQRLKIRADQQHLQSSIHPHVLRHSFASHVLQSSGDLRAVQEMLGHASISTTQ
                     VYTHLDFQHLAKIYDCAHPRAKRKKIAPSSD"
     gene            complement(1959..2972)
                     /locus_tag="PKZ49_08540"
     CDS             complement(1959..2972)
                     /locus_tag="PKZ49_08540"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HND36647.1"
                     /translation="MAIIKGWAALGPQQKLQPYTYEPGPLNGEEVEIAVEYCGLCHSD
                     LSMINNDWSLTRYPIIPGHEVVGRIIAIGDQVKGLKVGQSVGVGWNALSCMHCRECLT
                     GQHHLCPETQSTIVGRHGGFAERIRAHWVWVIPLPDTLDKSIAGPLLCGGITVFAPLQ
                     VFDVKPTDRVGVVGIGGLGHLALQFAHAWGCEVIALTSSPAKAEEAHKFGAYYSVSSR
                     DRSEISKLANTLDFLLVTVNVPLDWSSLLKTLKPNGRMHIVGAVLEPIPLQAFDLIMG
                     QKRISGSPTGGPTMTAMMLHFAARHRIAPQVEHFPLNQVNEAIAHLAAGKAKYRIVLD
                     HTN"
     gene            complement(3236..4489)
                     /locus_tag="PKZ49_08545"
     CDS             complement(3236..4489)
                     /locus_tag="PKZ49_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011113016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HND36648.1"
                     /translation="MKKKLVSLLVAGAMATPVITSAQVLNGVGGPGTHVTIFGRVQAE
                     YSTIDIDGKNAQTGILDDANQSRWGLHVSENLGMGLRAMGRIEYGLNPGSGRNDNARE
                     QWVGLGGDQWGELKFGRVQSPFKDFAGGHTIDPFAYTTLHANGSGGTMTASANGLGSG
                     DNSFVNSSARFDSAVVEGFSMSALLMPGDANRLNPQSFGGVGGYDPLNPNTGGEDGEW
                     DFQIAGKYSNKFDAHGIDIFGGYSRDNISDRMKAAGINDDEEIWRGGGKYSFADFTLR
                     GQYEYVNNANPLGGAATCSQAAALGSYGSLPSLLPSQTGDGGRGQCLSAMNANGDGYL
                     WFAGADYTIGNTKLVAQGGMAHAKAIGALDKRDVESLTVGVIHSLSRRSSLFGGYQRA
                     WVADRNATTTDSDRNVYTVGMRHDF"
     gene            complement(4800..6329)
                     /gene="recQ"
                     /locus_tag="PKZ49_08550"
     CDS             complement(4800..6329)
                     /gene="recQ"
                     /locus_tag="PKZ49_08550"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HND36649.1"
                     /translation="MQSNQADTLLRTVFGHGDFRGQQSEIINHVIQGGDCLVLMPTGG
                     GKSLCYQIPAILREGVGIVISPLIALMQNQVEHLRQRGVRVAYLSSLLAQSEIEAVEQ
                     DLQANCYDLLYVAPERLLTPRFLGLLQKIKLSLFAVDEVHCISQWGHDFRPEYVQLSI
                     LQKKFPHVPRIALTASADMRTRTEIIEKLDLYKARIFISSFDRPNIRYRVVNKANERQ
                     QLLHFIKSEHPRDAGIIYCLTRKKVEDVAFWLSSKDVAALPYHAGLSAQVRKNHHQQF
                     LSDKGKVIVATIAFGLGIDKPDIRFVAHLDLPKSIEGYYQETGRAGRDGLPASAWMTY
                     GFGDVARLRHLIENTGTQAMVKQVAMEKLDALLTFCEAVTCRRIRLLNYFGESVPQVA
                     CGNCDLCLDPPQVWDGTVAAQKALSCVYRAGQHSDLDHWIDVLCGKYTEQVKRWGHDK
                     ISTFGIGKELNEKDWRKLFRQLMVLGYLTSSRNDIYSQRLTPASRAILRGEQTLYLRT
                     K"
     gene            complement(6374..6763)
                     /locus_tag="PKZ49_08555"
     CDS             complement(6374..6763)
                     /locus_tag="PKZ49_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HND36650.1"
                     /translation="MIEVTDCEKFRIDKWLFAARFFKTRALAAEAVERGRVLVNGVRV
                     KPARTVVVGNRLIIRMDQCQYEIEVLALSNRRGSYAEAQKLYQETDASRQLRAAIMEK
                     AKAEPPQFYTKGRPTKRDRRELDRFKG"
     gene            7180..>8133
                     /locus_tag="PKZ49_08560"
     CDS             7180..>8133
                     /locus_tag="PKZ49_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HND36651.1"
                     /translation="MAVAEVLKKIQDHEVKFVDLRFTDTNGKEHHITVPAHTFTADKF
                     EDGHAFDGSSIAGWKGIHASDMLLMPDPETATLDPFMEETTLFLSCDVVDPSDGQGYN
                     RDPRSLAKRGEVYLQSTGIGDIAYFGPELEFFIFDSVRWQTTMSGCSLEIESEEAAWN
                     SKKKYEHGNTGHRPAIKGGYLPVPPVDSLHDIRSAMCVYLEEMGIDVEVHHHEVANAG
                     QCEIGTRFNSLVKRADWNQMLKYVVHNVANSYGKTATFMPKPIVGDNGSGMHVHQSIW
                     KDGKNLFSGNSYAGMSETALFYIGGIIKHTKALNAITNPGTN"
BASE COUNT         2383 a         1936 c         1554 g         2260 t
ORIGIN      
        1 aatctagtct ttgagtgaga tcttcatttg tgcaccacag aatcttcatt ttactataac
       61 tgatcgatct aaaatttact gtgatatcga tcatattcta aatcgctgcc caatgctcaa
      121 tcgtatactt tgatgagaac taagtgcgca gcattctatc aacaataaat tcaagccatc
      181 tggtcaatat acgctacctt taccactgtt ttcagtgtat atattacttc taattaatcc
      241 caaacactta cccagcttag cattttatgc gctaatatca tatgctattg ttatcgaatc
      301 gtgtaggaaa tgatctatga agccagaaga tgttgcacag tatttacagg aaaatcccca
      361 attctttgaa acgtatgctg atatgctcgc tgaaattaaa attccgcaac cttacgatgg
      421 gcgcgccatt tcaatcagtg agcgtcaaat aagcacttta cgggagaaga ataaattact
      481 gcaagaaaag ctgcatgaat taatcaatat tggagaaaac aacgatacga tcggcgaaaa
      541 aatgcatcgc ttggctgttt cattgcttga atttaatagc gtagaagaac tattgcatgg
      601 cttgaattac aatctttgcg aggacttttc gattccccat gtcgtcatgc gcttatggta
      661 cctcaatgat attaaaagca atcaaaaacg ccctgaattc agcgctgtga gtaaaacagt
      721 gagaatttct gtggagagca tgcgtaagcc ttattgtggc ccccaagtca gcgatgacat
      781 caaaccgtgg tttggccaag atgcaaaata tctgcaatct ttctctatta ttccgcttcg
      841 tcgcaaaaga accatcggtt tgttagtgat gggcagccct gacgctgagc ggttttaccc
      901 cgacatgggc acgctttacc tggagcgact cggtgaattg gttagtagcg ccctcgcacg
      961 actcgagcgc attgatcttg aacagataat tgatgagccc cccaaaattt gaagaaaaac
     1021 cggtaagcat tagtgcttac cttgcgtatt tacgccaagt ccgtcggtta gcaaagctaa
     1081 cttacaaaag ctatgaacgc gatttagcag tactcaatca attttcgatg agcatcccct
     1141 tccatgaatt agaggcacat gatattcgcc atttcatagc ccgattacat ggctcagggc
     1201 tatctggcaa aagccttgct agaacattat cagcttggag aactttttat caatacttga
     1261 ttcgatttcg tggttatcaa aataacccgt gccaaggcat caaagtgccc aagtcaccca
     1321 agcatctacc tcacacatta tcaccggatg aagcagctaa attaatggat ttcgagtcta
     1381 ccgatagcct cagcatacgc gatcgtgcca tgtttgaatt gtgctattca tctggattac
     1441 gcttacaaga gctagtccaa ctcaaaccgc atgatatcga ttttactgag gcacaagttc
     1501 gcgttgttgg caaaggcgag aaaacccgca tcgtaccggt aggtgattat gccttggcgg
     1561 cattaagcgc ctggctgaaa cagcgcgaag ctttaccgaa gctcgatagt gatgcgttat
     1621 ttgtctcgag tcgcggtaat cgaattcacc cacgaactgt ccagcagcgc ctcaaaatac
     1681 gcgccgatca gcagcactta caaagtagta tccatcctca cgttttacgt cattcttttg
     1741 cgtctcatgt attgcaatcg agtggagatc tacgcgccgt ccaagaaatg cttgggcatg
     1801 ccagcataag cacgactcag gtctatacac acctcgattt tcaacattta gcaaaaatct
     1861 atgactgcgc tcacccacgc gccaagcgaa aaaagattgc tccatcatca gactaaggcc
     1921 cagcagttac ccaacgaagc gcttgagatt actagaagct aatttgtgtg atctaacaca
     1981 atccgatatt ttgctttccc agcagcaaga tgggctatcg cctcgttgac ttgattcagt
     2041 ggaaaatgtt ctacttgcgg cgcaatgcga tgtcgagctg cgaaatgcag catcattgca
     2101 gtcattgttg gtccacccgt gggtgatccg gaaatacgct tctgccccat aattaaatcg
     2161 aatgcttgaa gaggaatggg ttcaagcaca gcccctacga tatgcattcg cccgtttggc
     2221 ttaagcgttt taagcaatga tgaccaatcc aaaggaacat tcacagtaac caataagaaa
     2281 tccaatgtat tagctagttt actgatttca ctccgatcac gactggatac actatagtat
     2341 gcaccgaact tatgagcttc ttcagctttt gcaggactcg aagtcaatgc aatcacctca
     2401 catccccagg catgcgcaaa ctgtagtgct aaatgcccta acccgccaat tccaacgaca
     2461 ccgactcgat ctgttggctt cacatcaaaa acctgtagtg gagcaaatac cgttatgccg
     2521 ccacagagca gtggccctgc tatggactta tctaacgtat caggcaatgg aattacccaa
     2581 acccaatgcg cccgaatccg ctcagcaaaa cccccatgac gccctacaat cgttgactga
     2641 gtctctgggc aaagatggtg ctgacctgtt aagcattccc ggcaatgcat acaactcagt
     2701 gcattccagc caaccccaac cgattgacca acttttagtc cttttacttg atctcctatc
     2761 gcaataatac gccctaccac ttcatgccca ggaataatcg gataccgagt caagctccag
     2821 tcattgttaa tcatcgacaa atcagagtga caaagcccgc agtactcgac agcaatttct
     2881 acctcttcgc cattcaaagg acctggctcg taagtgtaag gttgcaactt ctgctgaggc
     2941 cctaatgcag cccatccttt gattatggcc aaaattttct ccttgataat taaagcatgt
     3001 gtaagagaaa taataagact gattctctac ttaacacaag cgcacgattt aaccagtttt
     3061 cgcccctaag attaggttta gcgaaattaa ggtaatgtat cgttcaaatt agaaacaaag
     3121 caaggattca atttcagtca ttcattaagt aaaccctgag tactttaact tacgaaagtt
     3181 gcaataaaaa aggggtgcct tttgggcacc cctttctttt caaacttttc taatcttaga
     3241 aatcgtgacg catacccact gtgtaaacgt tacggtctga atcagtcgtt gttgcatttc
     3301 tatcagcaac ccaagcacgt tgataaccac cgaacaagct tgaacgtctg cttaaactat
     3361 gaatcacacc aaccgttagc gactcaacat cacgtttatc aagcgcaccg atcgctttag
     3421 catgagccat accgccttgt gctacgagct ttgtgttacc gattgtgtaa tcagcaccag
     3481 caaaccagag ataaccgtca ccatttgcgt tcatcgctga taaacattga ccacgtccac
     3541 catcacccgt ttgtgaaggc aataagcttg gcaatgaacc atagcttcct aaggctgctg
     3601 cttgtgaaca ggtcgctgcg ccgccgagtg ggttagcatt gtttacgtat tcatattgcc
     3661 cacgtaaggt aaaatcagca aaactgtatt taccaccacc acgccagatt tcttcatcat
     3721 cattgatacc agccgctttc atacgatcac tgatgttgtc acgggaataa ccaccgaaga
     3781 tatcgattcc atgagcatca aatttgttgc tgtatttacc agcaatttgg aagtcccatt
     3841 caccatcttc accaccggta ttaggattca aaggatcgta gccaccgaca cccccaaagc
     3901 tttgtggatt gagtctattt gcatcacctg gcattaacaa agctgacatt gagaaacctt
     3961 caacaaccgc agaatcaaaa cgcgctgaac tgttaacaaa actgttgtca ccagatccta
     4021 agccatttgc actggctgtc atggtaccac cactaccatt agcgtgtagg gttgtataag
     4081 caaacggatc aatagtatga ccaccggcaa aatctttaaa tggagattga acacgcccaa
     4141 atttcaattc gccccattga tcgccgccca gtcctaccca ttgttcacgg gcattatcat
     4201 ttctacctga acctggatta agaccgtatt cgatacgacc catcgctctt aaacccatac
     4261 caagattttc actgacatgc aaaccccagc gtgattggtt agcatcatcc agaatgcctg
     4321 tctgtgcatt tttgccatcg atatcaatcg tgctatattc agcttgtaca cgaccaaata
     4381 tcgtgacatg ggtaccaggt ccaccaactc cattcagtac ctgtgctgaa gtaataactg
     4441 gggtcgccat tgcacccgca actaacagtg aaactagttt ctttttcatc tgaaactctc
     4501 ctataaaatt agaacgacta ttcgccatgc ttaaaatctt tcgcttccat tctcgctgaa
     4561 tgttttcaat tatccagacc tttcgaactg gaaaggttag gcagattgtg cacaacacaa
     4621 gataatgatg caacaaaaaa caactaactt ttctgttttt aacaacattt gttgtttttg
     4681 aacaacattt gtgggcgcca aacgacttaa tttacgaaaa taaaaccaaa aaatacgcta
     4741 attcaatgct ttaaaaataa tgaatacgta aaaaaacgat aatttcaaat aaaagtcagt
     4801 tactttgtac gaagatagag cgtttgttct cctcgaagaa tggcacgact cgctggcgtt
     4861 agtcgctgcg agtaaatatc attgcgactt gacgtcagat aaccgagcac cataagctgc
     4921 cggaacaatt tccgccaatc tttttcattt aattccttac cgataccaaa cgtacttatt
     4981 ttatcgtgtc cccagcgctt aacctgttct gtatacttgc cgcacaacac gtcgatccaa
     5041 tgatcgagat ccgaatgctg cccggcacga tatacgcatg acaacgcttt ctgcgcagcg
     5101 actgtaccat cccaaacttg cggtggatca aggcaaagat cgcaattacc acaagccact
     5161 tgcggtacag attctccaaa atagttgagc aatcgtatcc gacgacacgt tactgcttca
     5221 caaaatgtca gcaaagcatc taatttctcc atcgcaactt gtttaaccat tgcttgcgta
     5281 cccgtatttt cgatcaaatg ccttaatcga gcgacatcac caaatccata ggtcatccag
     5341 gcgctggctg gcaaaccgtc acgaccagcc ctaccggttt cttggtaata cccttcaata
     5401 cttttaggca gatccagatg cgcaacaaaa cgtatatctg gtttgtcaat cccaaggcca
     5461 aatgcaatgg tcgcaacgat gaccttccct ttatcactca aaaattgctg atgatgattt
     5521 tttcttactt gagcactcaa tcctgcatga tagggtaatg cggctacatc tttgctcgat
     5581 aaccaaaagg caacatcttc aacttttttg cgggtcaaac aataaataat accggcatca
     5641 cgaggatgtt ccgatttaat gaaatggagt agctgctgcc gctcattagc tttattgacg
     5701 actcgataac gaatattagg tcgatcaaaa ctcgaaataa agatgcgcgc tttatacaaa
     5761 tccagttttt cgataatttc agtacgcgtc ctcatatcgg cagatgctgt tagcgctatt
     5821 cgaggcacat gcggaaattt tttctgcaaa atcgaaagtt gaacatattc gggcctaaaa
     5881 tcatgccccc attgagaaat acagtgcact tcatcaacgg caaacagcga cagcttaatt
     5941 ttttgcaata accccagaaa acggggtgta agcaatcttt cgggagcaac ataaagcaaa
     6001 tcatagcaat tagcttgcaa atcctgctca accgcttcaa tttccgattg agcaagcaag
     6061 gaactcaaat acgccacacg aactcctcgt tgcctcaaat gctcgacttg gttttgcatc
     6121 aacgcaatca aaggtgagat gactattcca acaccttcac gcaatattgc aggaatttga
     6181 taacacaagg atttaccccc gcctgtcggc atcaatacga ggcaatcccc tccttgaata
     6241 acgtgattaa tgatttctga ttgctgtcct cgaaaatcac cgtgcccaaa cactgttctt
     6301 aggagagtgt cagcctggtt cgattgcacg cttgatcgtc ctgtttatag ttttcttatg
     6361 atatttgacc aatctagccc ttgaatcgat ctaattctcg tctatcgcgt ttggttggcc
     6421 taccttttgt ataaaactga ggtggctcag ctttagcctt ttccataatg gctgcacgta
     6481 attgtcgact tgcatccgtt tcttgataaa gcttctgagc ctcagcatag gaacctcgtc
     6541 gattgctcaa cgctaatact tcaatttcat actgacactg atccatacga ataatcaatc
     6601 gattaccgac tacaaccgtt cttgctggtt tgactcgtac accattgaca agcaccctac
     6661 ccctctctac cgcttcagcc gctaatgcgc gggttttaaa aaaacgggca gcaaagagcc
     6721 atttatcaat acgaaacttt tcacaatcgg ttacttcgat catctcaatc caatttcata
     6781 tttatctgcg tggaagctcc tgagtagatt caattattgg tgccagatcc tatccctatg
     6841 ctatttagcg cactatatcc ttgtagtatc gataacagcg agggcaataa attacaaatt
     6901 ttgtaaaaga tgattttagt cattaataat caataagaac agtaagtcca gattcttata
     6961 taattagaag ctaaattcaa attttaagag ggtttgtagt ttgactttta tttactttaa
     7021 tactcgatta atcacgtaaa tacttgttta acccgacaca tcagagctaa ttaattagct
     7081 atatttaaaa tggttttttg aatattcagc actatatcaa ttaacaaatc attaattcac
     7141 catctaaaaa gaatcatcat tctgtctgga gaaaattaaa tggcggtagc agaagtttta
     7201 aaaaagattc aagaccacga agtaaaattt gttgatcttc gcttcaccga tacgaacggt
     7261 aaagagcatc atattactgt ccccgctcat acttttactg cagataaatt tgaagatggt
     7321 catgcgtttg atggatcctc aatagccggt tggaagggta ttcatgcttc agatatgctg
     7381 cttatgcctg accctgaaac tgctacttta gatcccttca tggaggaaac aacgctcttt
     7441 cttagctgcg atgttgttga tccttcagat gggcaaggct ataaccgtga tccacgctcc
     7501 ttagcaaagc ggggtgaagt ctacctccag tcgactggca ttggggatat tgcctatttt
     7561 ggtcctgaac tggaattctt catttttgat tctgttcgct ggcaaactac aatgtcaggc
     7621 tgctccttag aaattgaatc ggaagaagct gcctggaatt caaagaaaaa atatgagcat
     7681 ggtaatactg gacaccgccc tgctatcaag ggtggttatt taccggttcc ccctgtcgat
     7741 tctctgcatg atatccgttc tgcaatgtgc gtataccttg aagagatggg aatcgacgtt
     7801 gaggtgcatc atcacgaagt tgcaaatgct ggtcaatgtg agattggaac acgtttcaat
     7861 agtctcgtga aacgtgctga ttggaaccaa atgctcaaat atgtggtgca caatgtggct
     7921 aattcttacg gcaaaacagc cacgtttatg cctaaaccga ttgtaggaga taatggttcg
     7981 ggaatgcacg tgcaccaatc tatctggaag gatggtaaaa atttattttc aggaaatagc
     8041 tatgcgggca tgtctgaaac ggccctattc tatattggcg ggatcattaa gcataccaag
     8101 gcattaaatg cgattaccaa tccgggaaca aat
//
LOCUS       DAOQKQ010000117         8105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_4319_length_8105_cov_6.427826, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000117 DAOQKQ010000000
VERSION     DAOQKQ010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 8105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8105
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4319_length_8105_cov_6.427826"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="PKZ49_08565"
     CDS             complement(<1..519)
                     /locus_tag="PKZ49_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36652.1"
                     /translation="MDNNQINQTKSEDETDSLNNCIQYARTILNEQLARIKRKKYDFA
                     PEFKFMTIQLYLVGVMWQFYEKQQQMAPEVAREKAFEALHSMMIKDGAKPKRAEKQIT
                     FIKEMAKLEDGDEALAIHIGYESQPEDESLSEVFDHYVDEPRVSGALWRSYDRGKKII
                     LLGGLLVGMAAVW"
     gene            770..2527
                     /gene="argS"
                     /locus_tag="PKZ49_08570"
     CDS             770..2527
                     /gene="argS"
                     /locus_tag="PKZ49_08570"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HND36653.1"
                     /translation="MTVPKQNNFKVHFTELLYQATEKILPPNCEIFLELARPKQVEHG
                     DYSSNIALQLARFLHKNPKEIAQSLIQLLPDSPYLEKAEVAGNGFINLFLKKSAKQQF
                     LLQVLQQGSFFGTTDIGKGNQIQVEFVSANPTGPLHVGHGRGAAYGASLANILRANGY
                     SVTREYYVNDAGRQMDILTLSTWLRYLELCGVSIPFPGNAYQGEYVIDMAKMIKKAHG
                     DRYVHEPHALIARVTPASESSISNEDEQLDELIQVAKKTLSEDYAYIHQFVLTEQLGD
                     CRNDLMEFGVEFDTWFSEQSLFDNGSIAKVIAMLEKNNMLYQQDSALWFRSTRFGDEK
                     DRVVQRENGQYTYFASDIAYHLNKFERGFSHIINVWGADHHGYIPRVKGAMQALSLDP
                     KKLEIALVQFAVLYRDGKKASMSTRSGEFVTLRELRKEVGNDAARFFYVLRKSDQHLD
                     FDLDLAKSQSNDNPVYYVQYAHARINSVITQWGEAVSILSNAPTDSLSNEAELTLIQK
                     LIDYPDTLEAAAKERAPHFLAFYLKDLASAFHSYYNSTRFLVPEQELKLARLALIAAI
                     QQVIANGLNLLGVTAPNEM"
     gene            2548..3177
                     /locus_tag="PKZ49_08575"
     CDS             2548..3177
                     /locus_tag="PKZ49_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HND36654.1"
                     /translation="MSRDYKSRGGASSKGGGSLILGIFIGYALGLVSAIGVWLYINQA
                     PSPFLTEDKTANRPKEELLSKPTQTEKSVVSNTNNNNSTEESKSRFDFYKILPGIDEP
                     DSGNVPNAKEIAKASPQAESVQRGANSYFLQIGSFKSAHEAEKVKAELAFLGMMAAIQ
                     PTESSDKGTWYRVRVGPFSDIHEIDQIRASLQENQIESNIIRLPTSNMP"
     gene            3232..3879
                     /locus_tag="PKZ49_08580"
     CDS             3232..3879
                     /locus_tag="PKZ49_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HND36655.1"
                     /translation="MKKLTKLFILLLIGCVSSVGVSVASTEIVEGKDYTVLSTPQATD
                     DARSIEVIEFFWYGCPHCYDLHPHIDQWRKNAPKDVKFRYVPAIFRANWTVAAKTFYA
                     MEALGISKDLHDKVYDAIHAKKIDLAKESTLFEWVEKQGVDRKEFVNAYNSFSVQNMV
                     NRASQMTKQYKLTGVPALVVEGKYLTSGKMTGLPRDTILVLDALIEKARKEKSSQ"
     gene            3991..6291
                     /locus_tag="PKZ49_08585"
     CDS             3991..6291
                     /locus_tag="PKZ49_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HND36656.1"
                     /translation="MGVANKSMARKSSDNPLPPKLAKLLRESGCLVLGAIALYLVLIL
                     LSFNRADPGWSHSENLNQITNTGGKAGAWLSDLLLYLFGASAWWLVALFIAAIIWSYR
                     RIDIVSLFDPRSLIISLIGFVALLMASSGIEALRFNTLNIALPLAPGGILGETISQGF
                     SRLLGVTGASLSFFILFAIGFSLFSGISWVRIAEKIGETIELSCVTLWQSWLTWLDRR
                     AGKLSSLERSEHVDDLKKDLSTHPPLKIEIPDTSIPKSSRILKEKQTPLFSELPDSIL
                     PPLYLLDEPKVDVELLSNETLEFTSRLIERRLQEFGVEVKVVSAHPGPVITRYEIEPA
                     TGVKGNQIINLVKDLARALSVASIRVVETIPGKTTMGLEIPNPKRQIVRLHEILGSQA
                     YADTESPLTIALGKDISGRPIVSDLGKMPHALVAGTTGSGKSVAINAIILSLLYKASP
                     EQVRLILVDPKMLELSVYEGIPHLLTPVVTDMRDAASALNWSVAEMERRYKLMSALGV
                     RNLAGYNQKIQEAKKAGQPLVTPTHIVSDDATPLEEMPMIVVVIDELADLMMVMGKKV
                     EKLIARLAQKARAAGIHLLLATQRPSVDVITGLIKANIPTRIAFQVSSKIDSRTILDQ
                     MGAEALLGQGDMLYLPPGSGYPQRIHGAFVADHEVHKVVEYLKQQGEPRYIEAVLKAG
                     DEEGDFNGESSENNRPSGETDLLYDEAVAIVLKSRRASISMIQRQLRIGYNRAARLVE
                     DMERAGLVSAMQSNGNREVLAPDRGD"
     gene            6349..6963
                     /gene="lolA"
                     /locus_tag="PKZ49_08590"
     CDS             6349..6963
                     /gene="lolA"
                     /locus_tag="PKZ49_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HND36657.1"
                     /translation="MIRFVIVVVLLFLSLAAQASAVEHLREFVQKTQTFKAVFHQKLL
                     DQNFQVVQEASGSMMFQRPGKFRWIYEKPYQQLIVGDGKRVWFYDQDLNQVTVRQLDL
                     AIGNSPAALLAGSSAIENDFNLAEIQIEDEIDWLEATPKHKETNFELIRMGFAKTGEL
                     REMILRDNFGQFTLLVFSEINFNPNLASELFQFDPPSGADVISD"
     gene            6956..>8105
                     /locus_tag="PKZ49_08595"
     CDS             6956..>8105
                     /locus_tag="PKZ49_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HND36658.1"
                     /translation="MTELDFSSRPPLSPPLAERLRPRSLEEVIGQTHLLGIGRPLRNA
                     FETGKPHSMILWGPPGSGKTTLARLMSHSFDAEFIAISAVLSGVKDIREAIDRAKSIY
                     QQSQRHTVLFVDEVHRFNKAQQDAFLPYVEQGLITFIGATTENPSFEVNRALLSRAQV
                     YTLAPLSQEELQQLFDRTHQSVLKTLNFSYEAKQLIIQFADGDARRLLNLLEQLSIAA
                     EAEAYNTIDTNEVRKALTRSQRNFDKGKEAFYDQISALHKSIRGSSPDAALYWMCRML
                     DGGVDPMYIARRLIRAAVEDIGLADPRALTMTLDACQAYERLGSPEGELALAQATLYL
                     ACAPKSNAAYLAYKKARSMIEHDTSHPVPLHLRNAPTQLMQEMGYGKQY"
BASE COUNT         2360 a         1719 c         1713 g         2313 t
ORIGIN      
        1 ccataccgct gccatcccaa cgagcagtcc acccaataaa ataattttct tacctcgatc
       61 ataggatcgc catagtgcac cggaaacccg cggttcatca acataatgat caaagacttc
      121 ggataagctc tcgtcttctg gttgagactc gtatccaata tgaatggcta aagcctcatc
      181 gccatcttca agttttgcca tctccttgat gaaggtaatt tgtttttcgg cacgcttagg
      241 ttttgcacca tctttgatca tcatcgaatg gagcgcttca aaagcctttt cacgcgccac
      301 ttcaggtgcc atctgttgct gtttttcata aaattgccac atcactccaa ccagataaag
      361 ctggatcgtc atgaatttaa attcaggcgc aaagtcatat tttttacgtt tgattcgagc
      421 cagctgctca ttcagaatcg ttcttgcgta ttggatacaa ttatttaagg aatcagtttc
      481 atcctcgctt tttgtttgat ttatttgatt attatccata ttgcaacctt taatttttct
      541 tagccaatta tttaatgtgc actattatat acttgcaagc gatgtaaatc acattactgt
      601 ctcgttaagt gctgatgaga aggttgatac ttgatgcgaa ataaccaata acgttctctt
      661 cagaagaaaa tggctcgtta tgaatattaa ggttagttta aacaaatcac tcgcataaaa
      721 tcaccttatc ctcgatataa tttacgtttt acttaaatct cttttgctca tgaccgtccc
      781 caaacaaaat aattttaaag ttcatttcac tgaattactc tatcaagcaa ctgagaaaat
      841 attaccgcct aactgtgaaa tatttctgga gctggcacgt cctaaacaag tagaacatgg
      901 tgactattcc agcaatattg cgctgcaatt agctcgtttt ttgcacaaaa atcctaaaga
      961 aatcgcccaa tcgctcattc aactacttcc agattcccct tatttggaaa aagcagaagt
     1021 tgcgggaaat ggttttatca atttatttct caaaaaatct gcaaagcagc aatttttatt
     1081 acaggtatta cagcaaggca gtttttttgg gactaccgat attgggaaag ggaatcaaat
     1141 tcaggtcgag tttgtatcag caaatccaac aggccctttg catgttggtc atggaagagg
     1201 tgctgcatat ggtgcaagcc ttgcaaatat cttacgtgca aatggctatt cggtaacccg
     1261 tgagtattat gtaaatgatg cgggaagaca gatggatatt cttacattat caacctggtt
     1321 acgctatctc gagctttgtg gtgtatccat cccatttcct ggaaatgctt accagggaga
     1381 atatgttatc gatatggcca agatgataaa aaaagcgcat ggcgatcgat atgtacatga
     1441 accgcatgca ttaatagcac gggtcacccc ggcaagtgag tcatcaatct cgaacgaaga
     1501 tgaacaactc gatgaattga ttcaggtagc aaaaaaaact cttagcgaag attatgccta
     1561 tatacatcag tttgttttga ctgaacagtt aggggactgc cggaatgatt taatggaatt
     1621 tggtgttgaa tttgatactt ggttttctga gcaatcactt tttgataatg gatcgatcgc
     1681 aaaagtaatc gccatgctcg agaaaaataa tatgctctat cagcaagata gtgcactttg
     1741 gtttcgttca actcgatttg gtgatgaaaa agatcgtgtc gtccagcgtg aaaatgggca
     1801 atatacctat tttgcttccg atatcgctta tcatctcaat aaattcgagc gtgggttttc
     1861 tcatatcatc aatgtatggg gggccgatca ccatggttat atcccacgag tcaaaggcgc
     1921 aatgcaggca ctatccctcg atcccaagaa gttggaaatt gcattagtac aatttgccgt
     1981 gctttatcgg gatggcaaga aagcctccat gtctacccgc tccggtgaat tcgtcaccct
     2041 aagagaacta cgcaaggaag tcggtaatga tgcagcacga ttcttttacg tattacgaaa
     2101 aagtgatcag catttagatt ttgatttgga cctcgcaaaa tcacaatcga atgataatcc
     2161 ggtttactat gtacaatatg ctcatgctcg gataaatagc gttattacgc aatggggtga
     2221 agcagtttcg atactttcca atgcgccaac tgattcgtta agcaatgaag cggaacttac
     2281 tttgatacag aaattaattg attatcctga tacacttgaa gctgctgcaa aagaacgggc
     2341 ccctcacttc cttgctttct atcttaaaga tctggcgagt gcgtttcata gttattataa
     2401 ttcaacgcgc tttcttgtac cggaacagga acttaagtta gcaaggctgg cattaattgc
     2461 agcaatccaa caagttattg ctaatgggtt aaacttatta ggtgtaacag ctcccaacga
     2521 aatgtaactg cagttttata gaacattatg agtagagatt ataaatcaag gggaggagca
     2581 tcatcaaaag gtggtggttc gcttattctt ggtattttca ttggctatgc actgggttta
     2641 gtcagcgcaa ttggtgtatg gctctatatc aatcaagcgc ctagtccttt tttaacggaa
     2701 gataaaacag caaaccgacc gaaagaagaa cttttatcca aaccaaccca gacagaaaag
     2761 agtgtcgtta gcaacaccaa taacaataac tcgacagaag aaagtaagtc tcgctttgat
     2821 ttttataaga tacttcctgg aatcgatgaa cccgattcag gaaatgtgcc aaatgctaaa
     2881 gaaatagcaa aagcttctcc ccaagctgag agcgttcagc gtggggcaaa tagctatttc
     2941 ttacagatcg gttctttcaa gagtgcacat gaagctgaga aagtgaaagc tgaacttgca
     3001 tttttaggta tgatggctgc catccaaccg actgaatcat cagataaagg cacctggtat
     3061 cgcgttcgtg tggggccatt ctcggatatc catgaaattg atcagattcg ggcttcctta
     3121 caggaaaatc aaattgaatc aaatataatt cgattaccaa cgagtaatat gccttaatgg
     3181 atatgttatc ctaaaatgat ttctaacatt ccttttacca tttgcgatgc tatgaaaaaa
     3241 ctaaccaagc tttttatctt gcttttgatt ggctgtgtaa gcagtgtagg cgtatcagta
     3301 gcttctactg aaattgttga aggcaaagac tataccgttc tttcaacacc acaagctacc
     3361 gatgatgccc gcagtatcga agtcatcgag tttttctggt acggctgccc acattgctat
     3421 gatttgcatc ctcatatcga tcaatggcgc aaaaatgcgc ctaaagacgt aaaattccgc
     3481 tatgttccgg caattttccg tgcaaattgg accgttgctg ctaagacatt ctatgccatg
     3541 gaagcactcg gtatttcgaa agatttgcat gataaagttt atgatgcgat tcatgccaaa
     3601 aaaatcgatc tcgctaaaga atcgactttg tttgaatggg tagaaaaaca aggcgttgat
     3661 cggaaggaat ttgttaatgc ctataactct ttctctgtgc aaaatatggt caaccgtgca
     3721 tctcaaatga ccaaacaata taaattaaca ggtgtacctg cactggtagt cgaaggaaaa
     3781 tatctaacaa gcggtaaaat gactgggtta ccacgtgata ccattcttgt tctcgatgcg
     3841 ttaattgaga aagcccgcaa ggaaaaatca tcgcagtaag attaatcaag gagtgccctg
     3901 ctatcacatt tgagcgaccg tgaatgtgat gataatgaag tagcagtgat ataatctgca
     3961 ggtcactgct tacctttccc aggaattcac atgggcgttg ccaataaatc aatggcgagg
     4021 aaatcgagcg ataatcctct gcccccaaag ttagctaaat tattacgtga gtcgggttgt
     4081 ctggttctag gcgcgatagc gctatatctt gtactgatat tgttaagctt taatcgagct
     4141 gatccgggtt ggtctcacag cgaaaatctt aatcaaataa ccaacacagg cggtaaagca
     4201 ggtgcatggt tatccgattt attgctatat ttatttggtg cctcagcttg gtggttagtc
     4261 gctttattta tcgcagcgat tatctggagt taccgacgaa tagacatcgt cagcttattc
     4321 gatccacgct ctttaatcat atcgttgatc ggttttgtgg ctttgttgat ggcaagtagt
     4381 ggcattgagg cactacgatt taataccttg aatattgcat taccgctagc accaggtgga
     4441 atattaggtg aaacgattag ccaaggtttc tcacgattac ttggagtaac cggcgcttca
     4501 ttaagttttt tcattttatt tgcaattgga tttagccttt tctcaggaat ttcctgggta
     4561 cggatcgctg agaaaatagg tgaaaccatt gagttatcct gcgttacttt atggcaatca
     4621 tggttgacat ggctagatcg gcgggcaggg aaattatcct ctttggaacg aagtgagcac
     4681 gttgatgatt tgaaaaaaga tttatcaacc catcctcccc tcaaaataga aatcccagat
     4741 acatcgattc ccaaatcgtc tcgtatcctt aaagaaaagc agacgccatt attttcagag
     4801 ctcccagact caattcttcc ccccttatat ttattggatg agccgaaagt tgatgtagaa
     4861 ctcttgtcga acgagacctt ggaattcact tcccgcttga ttgagcgaag gttacaagaa
     4921 tttggggtag aggtaaaagt cgtatcggcc catccgggcc ctgtcattac gcgttacgag
     4981 atcgaaccag cgactggcgt aaaaggcaat caaattatta acctcgttaa agatttagca
     5041 cgcgcacttt cagtcgccag tattcgtgtt gtagaaacta ttccaggcaa aacaaccatg
     5101 ggattagaaa tccccaaccc taaacgacaa atcgtgcgtt tacatgaaat tctcggttca
     5161 caagcttatg ctgatacgga atcacctttg actattgcac tcggtaagga tatcagcggt
     5221 cggcctatcg tctccgattt aggtaagatg ccgcacgcat tagtggctgg aacaaccggt
     5281 tcaggtaaat ccgttgcgat caatgcgatc attttaagtt tgctctataa agcatcacca
     5341 gaacaagtaa gattgattct ggtcgatcct aaaatgcttg agctttccgt atatgagggc
     5401 attccacatc tgctcacccc tgtggtaact gatatgcgcg atgcggcgag tgcattgaat
     5461 tggtctgtag cagaaatgga acggcgctac aagctgatgt ctgcgttagg tgtacgtaac
     5521 ctcgccggct acaatcagaa aattcaagag gctaaaaaag ccggtcagcc gctggtaaca
     5581 ccgacccata tcgtatccga tgatgcgaca ccactcgaag aaatgcccat gatcgtggtc
     5641 gtgatcgatg aattggctga tttgatgatg gtcatgggta aaaaagttga gaaacttatc
     5701 gcgcggctgg cccaaaaagc ccgtgcagcg ggtatccatt tattacttgc tacgcagcga
     5761 ccttccgttg acgtcattac tggtttaatc aaagcaaata tcccaacccg aatcgcattc
     5821 caagtttcga gtaaaattga ttcacgtacc attctggacc aaatgggtgc ggaagcgtta
     5881 ctcggtcaag gtgatatgct ttatctccca cccggaagcg gttacccaca gcgtattcat
     5941 ggtgcatttg tagcagacca cgaagtccac aaagtagtgg aatacctcaa gcagcaaggt
     6001 gaaccacgct atattgaggc tgttctcaaa gcgggtgatg aagaaggcga tttcaatggg
     6061 gaatccagtg agaataatcg accttcggga gaaactgatt tgctgtatga tgaagcagtg
     6121 gcaatcgttt tgaaatcacg acgcgcatcg atctcaatga ttcagcgaca acttcgtatc
     6181 ggttacaatc gagcagcacg cttagttgaa gatatggaac gcgctggtct cgtctctgct
     6241 atgcaaagta atggaaatcg tgaagtactc gcgcccgacc gcggtgatta agcaatgccc
     6301 gttatggata atattattaa actcgtcatc gctattttgt agggggttat cattcggttt
     6361 gtaatcgtag tcgtcttatt gtttctttct ttagcggcac aagcttctgc tgtagaacat
     6421 ctaagagaat ttgtgcagaa aacacaaact tttaaagcag tctttcatca gaaattactc
     6481 gatcaaaatt ttcaagtcgt tcaagaagca agtggctcaa tgatgtttca acgtcctggt
     6541 aaatttcgct ggatttatga gaaaccctat caacaattaa tcgttggtga tggtaagcga
     6601 gtatggtttt atgaccagga tctcaaccaa gtaacagtgc gccagcttga tctcgcgatt
     6661 gggaatagcc ctgctgcgct acttgcgggt agcagcgcaa ttgaaaacga ttttaatctt
     6721 gcagaaatcc aaattgaaga tgaaattgat tggttggaag caacaccgaa gcataaggaa
     6781 accaactttg aattgattcg gatgggtttt gcaaaaacag gtgaactgcg cgaaatgatt
     6841 cttcgcgata attttggtca atttacccta ctggttttta gtgaaataaa tttcaaccct
     6901 aatcttgcta gcgagctgtt tcagtttgat ccacccagtg gggctgatgt aatcagtgac
     6961 tgagcttgat ttttcttctc gaccaccctt atccccaccg ttagcagaac gcttacggcc
     7021 tcgatcactc gaagaagtaa tcggccaaac tcacctcctc ggtatcggta ggcccctacg
     7081 taatgccttt gaaacgggaa agccccattc gatgattctg tggggacctc ctggtagcgg
     7141 aaaaacgacg ctagctcgcc tgatgtccca ttcgtttgac gccgaattta tcgcgatatc
     7201 tgccgtttta tccggcgtca aagatattcg tgaagcaatt gatcgtgcga aaagtattta
     7261 ccagcagtca caacgtcata ctgtgttatt tgtagacgaa gtccatcgct ttaataaggc
     7321 acaacaagat gcctttttac catatgtaga acaaggttta attaccttca tcggggcaac
     7381 aaccgaaaat ccatcctttg aagttaaccg cgctttatta tctcgcgccc aagtttatac
     7441 actagcccct ttatctcagg aagaactaca gcagcttttt gatcgtacgc atcaatccgt
     7501 attaaaaaca ctgaactttt catatgaagc taagcagttg attattcaat ttgcggacgg
     7561 cgacgccaga cgattgctca atttactcga acagcttagt atcgccgctg aagcagaagc
     7621 ttataacaca attgatacca atgaagtacg caaagcgtta acacgaagtc agcgtaattt
     7681 tgataaaggt aaagaagcat tttatgacca aatttccgca ctccataaat ccattcgcgg
     7741 ttcttccccg gatgcggcat tgtattggat gtgtcgcatg ctcgatggag gcgtcgaccc
     7801 catgtatatc gcacgaagat taattcgagc tgcagtagaa gatatagggc tagctgatcc
     7861 acgcgcctta accatgacac tcgatgcatg ccaagcttac gaacgattag gaagtcctga
     7921 aggtgaactt gcattagccc aagccacgct ttatttagcc tgtgccccta agagcaatgc
     7981 tgcttaccta gcctataaaa aagcccgatc catgatcgag catgatactt cccacccagt
     8041 accactgcat ctgcgcaatg cgcctacaca attaatgcaa gaaatgggct atggaaagca
     8101 gtacc
//
LOCUS       DAOQKQ010000118         8093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_4326_length_8093_cov_6.350709, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000118 DAOQKQ010000000
VERSION     DAOQKQ010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 8093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8093
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4326_length_8093_cov_6.350709"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="PKZ49_08600"
     CDS             complement(<1..697)
                     /locus_tag="PKZ49_08600"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HND36659.1"
                     /translation="MKARGRQTVRVDYRMRWHSVMLALLILLYFAYSRFSVASDIKPN
                     LNYCSEQQAQIVAVEGIAEVLRSGERAWKHIRIGDGLCAGDMLRVRAHSRVALRQKNE
                     SMLRLGEKTTVTFAQSEVHSAQRKATLLELLQGVLHVITRTPEPFEIRTPFMNAGVDG
                     TEFSVAVEPDKTQVVVYEGQVTASNDQGSIVLADQEAGIAYKDQKPQKEVIVRPLDAV
                     QWALYYPAIIDSRV"
     gene            complement(894..3239)
                     /locus_tag="PKZ49_08605"
     CDS             complement(894..3239)
                     /locus_tag="PKZ49_08605"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HND36660.1"
                     /translation="MQKYGYGKKLLISFFIGLIGVVLYLLPFGLALDEKFGLNVLFHL
                     RGVTSVPSDVVVIAIDQYSASKLKDNNQIANNTIDPSKWPRALHAELIDRLIEEEVKV
                     IAFDLRFRGCGSSFEDNDRLVQAIKNAGNVILVEELVDSRDIADFPYQMDEQPEVCNS
                     ESLSTKDLNRDEPHQYYFSESVGLHGAWHRSVLPMISDAAQATAPFVLPRGVVAYHWT
                     FKEDAGGIPLLPAVVLQIFAQDVYDQFAHILQEIDPVAGLNLPSTLDKGIEIEALMYE
                     LRGQFIEKPALKDQFINALNQNDEIDTQTKGKLQALAELYAGHEKHYLNFYGPPRSIR
                     TIPYYQILNQDRLQDQRVDLRGKAVFVGVSAAKFGEQDKLRDDYTTVFKQLDGLQISG
                     VEIAATAFANLLENQTLHPIDEGYAAILVFLFGFMICVFYFALPYRIVFIAVILFLLI
                     YYFSAYQLFETKHLWIPLVIPLIQVGLGFGIALSLKYVESNQQKERLQQAFGKYVPDK
                     VVNDFIHNSGLATARGQQLYGVCMDTDADRYTELSEKMRPTELMVLMNEYYSRLFESV
                     KACEGLISDVKGDAMLAIWTEPNFSKRQCEQACRAALKIIDAVEVFNRSHTDRALPTR
                     IGLHIGEVVVGNVGTVDHYEYRAVGDLVNSANRIQSANKHFQTRILLSTDVLEGLDLF
                     LTRPLGSVLLHGKSNPINLAELINFKAKATDDQIWLCEAFAKVIQAYEVQNWIVAKQD
                     LLEILEKFPNDGPSRFFLNRCETYLKVSPVAWDAITYLSGK"
     gene            3459..3680
                     /locus_tag="PKZ49_08610"
     CDS             3459..3680
                     /locus_tag="PKZ49_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36661.1"
                     /translation="MKKLMAIALSAMLVAGSTFVFASGNKESKLEPQRAQEILNQMGC
                     KDKKPEETIKDRTDGSIVKCGDVKPLVKR"
     gene            complement(3800..4510)
                     /locus_tag="PKZ49_08615"
     CDS             complement(3800..4510)
                     /locus_tag="PKZ49_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HND36662.1"
                     /translation="MYTTSGIIDKEQFVVEFAPLVKRIAHHMMMKLPASVEVDDLIQA
                     GMIGLLDAINRYEGAYGRQFESYAAQRIRGSILDELRESDWLPRSLRKKMRQIEVAMR
                     SLEQKLGCPPSEQEIAKELNISLEEYHEALQDARGGQLIYYEDFQDDDEDHFLDRLCS
                     DSGNEPLEILVDKNLRELLVAAIDKLPIREKEVMGMHYEQEMNLREIGEVLGVSESRV
                     CQIHSQAIARLRAKLRNQ"
     gene            complement(4520..5407)
                     /locus_tag="PKZ49_08620"
     CDS             complement(4520..5407)
                     /locus_tag="PKZ49_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HND36663.1"
                     /translation="MSRKMIDQASGLRELAAMQARDSVKVFTVTGGKMGVGKTSIVVN
                     LAAALAKIGNRILILDENQHHNNVSANLGVKSHYDLTHLIKREQSLDQILAKAPDDIL
                     VLTAARGIHSLTQLSAEEQEWIIKCLCELPIDVVLVDTMIGEMGHVLPLSLASQQVLI
                     TVSGSSKSITDAYTLIKIMSQEYARQDFLVIVNKVKSELDAQSIYENIAKVAKQHLSV
                     RLEYLGCILFDEKLHRASQLCRSVVDAFPASTAAIEFKRLAEKLLRCSYSNQYSGGVD
                     NFMYRLIRTSHLSKVKLSV"
     gene            complement(5400..6665)
                     /gene="flhF"
                     /locus_tag="PKZ49_08625"
     CDS             complement(5400..6665)
                     /gene="flhF"
                     /locus_tag="PKZ49_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HND36664.1"
                     /translation="MKMRRFIAKTSHEALRNVKEELGADAVILSNKQVDEGIEIVALA
                     SNEMSSLVNSPESKFVPTPPPVEKKEEVLSVINPNIEVLEAIQQVKKESNISEALALN
                     IINEIQAMRHTLEDQLNSVAWGSLSQSKPETMKLMRTLLDAGFSPLLSRHLLEKLTKV
                     SNYEESLKQTVALLTLNLRTATSDQLIENGGVYALIGPTGVGKTTTIAKLAARAVIRH
                     GADKVALLTTDSYRIGGHEQLRIYGKLLGIPVRSIKDIDDLQLTLLELRSKHIVLIDT
                     VGMSQRDQMVSEQMALLRDCGTEIKHLLLLNATSNGGTLDEVISAYQYNGIEGCIITK
                     VDEAVGLGTALDVVIRRKLVLHYVTNGQKVPEDLHAANSRYLLHKIFKPIAENSPFSL
                     QDPEFALVMAGYDRNSAAKPKGYAEASYE"
     gene            complement(6662..>8093)
                     /locus_tag="PKZ49_08630"
     CDS             complement(6662..>8093)
                     /locus_tag="PKZ49_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HND36665.1"
                     /translation="LVVGVFQHDLDVSAAAKYYTILTIGDGLVAQIPSLIISTAAGMV
                     VSRVSSDQDLGQQVVSQLFNKPQVLFLTAGILGVMGLIPGMPNFVFLLLASIIAGIAY
                     FKVNYLPEPEAVTEEATSSPVAETEGREASWNDVMPVDMVGLEVGYRLIPLVDKTQQG
                     DLLKRINGIRKKFAQEVGFLPPVVHIRDNLELRPNAYRITLKGSVIGQGESYNGMYLA
                     INPGQATKTLSGVNTSDPAFGLPAVWIEPALKDDAQAHGYTVVDPSTVVTTHVNHLMR
                     SYAAELLGREELQKLFDHLGRSIPKLTEDLIPKLLPLATVQKILQNLLEEGVHIRDVR
                     TIVDVLTEHATRTQDTNELTALVRVALGRSILEQFFPSGEELQVMSLHHNLESILVQV
                     AQSGLVAGAGIEPGLADTLLKEVSKAVDQHEQLGIPAVLLVPDSIRLLLARFLRRPLP
                     QLKVISHAEVVDSRKIKFVSIVGGNK"
BASE COUNT         2361 a         1875 c         1474 g         2383 t
ORIGIN      
        1 tgacgcgaga atcgatgatc gctgggtaat ataacgccca ttgcaccgca tcgaggggtc
       61 gaacgatgac ttctttttgg ggtttctgat ctttgtaggc aattcctgct tcttggtcgg
      121 ctaatacaat gctaccttga tcattgcttg cagtaacttg accttcataa accacaacct
      181 gggttttatc gggctcaact gctacactaa attcagttcc atccacaccc gcattcatga
      241 aaggtgttct aatctcaaac ggttcaggtg tgcgggttat aacgtgcaat acaccctgca
      301 gtaattccag cagtgttgcc tttctttgtg cagaatgcac ctcagattgt gcgaaggtca
      361 cggtcgtctt ttcacctaag cgcaacatac tttcattttt ttgccttaac gccactcggc
      421 tatgagcgcg caccctcaac atatcacctg cacacaatcc atcaccgatc ctgatatgct
      481 tccaggctcg ttcccctgac ctaaggactt cagcaatgcc ttctacggca acaatctgag
      541 cttgttgctc tgaacagtag ttcaaattag gcttgatgtc acttgcaact gaaaaccgtg
      601 aataagcaaa ataaaggagt ataagtaacg cgagcatcac agaatgccat cgcattcgat
      661 aatctactct aactgtttgc ctccctctcg ctttcataat tgcaacctaa tcaatattat
      721 tcatttggtg aagatcgttt gccttcttat tttgcttctc cgagagaaac catatctgca
      781 ttattgataa tagcatatcc gatccttttc attcagcgtg tgatttttct cactgattct
      841 ttttataact aagtatgatg atagttttaa gacatagctt ttaaatcgat catctatttt
      901 cctgaaagat aggtgattgc atcccaagcg acgggtgaaa ctttcaaata tgtttcacaa
      961 cgattcaaaa aaaaccgaga aggaccatca ttagggaatt tttctagaat ctcgagtaag
     1021 tcttgttttg caacaatcca attctgaact tcataggcct gaataacctt agcaaaggcc
     1081 tcacaaagcc aaatttggtc gtcagtcgcc ttcgctttaa agttaatcaa ttcagcaaga
     1141 ttgattgggt tcgattttcc atgcagcaac acgcttccca aagggcgagt cagaaaaaga
     1201 tctaatcctt cgagtacatc agtagaaagc agtatccgag tttggaaatg tttatttgcg
     1261 ctttggattc gattggctga attcactaaa tcacccactg cgcgatattc ataatgatcg
     1321 accgttccaa catttccgac cactacctcc ccaatatgaa gtccaatccg ggtaggtagc
     1381 gccctatcag tatggcttcg attgaatact tcgactgcat cgataatttt aagggccgct
     1441 ctgcaggctt gctcgcactg tcgcttcgaa aaattaggct ctgtccaaat cgcaagcatg
     1501 gcatcacctt ttacatctga aatgagcccc tcacatgctt taacagactc aaataacctt
     1561 gagtaatact cgttcatcaa gaccatgagt tcagtcgggc gcatcttctc acttaattcc
     1621 gtataccgat cagcatcggt atccatacag acaccgtata actgctgccc tctagccgtt
     1681 gccaatcccg aattatgaat aaaatcattc accactttat caggtacata tttcccgaag
     1741 gcttgttgaa gccgttcttt ttgctgattc gattcaacgt attttagtga taaagcaata
     1801 ccaaacccta agcctacttg tatcaaagga atcactaatg gtatccacaa atgcttagtt
     1861 tcaaacaact gatacgctga aaaataataa attaagagga acaggataac tgcaatgaaa
     1921 acgattctgt atggtaaggc gaagtaaaaa acgcaaatca taaaaccaaa caaaaacacg
     1981 agtatcgctg catacccttc atcaatggga tgtaatgttt ggttttctaa caagtttgca
     2041 aaagcagttg ctgcaatttc taccccactg atctgcaacc catccaattg tttaaaaaca
     2101 gttgtatagt cgtctctcag tttatcttgc tcaccaaact tagctgctga tacacccaca
     2161 aaaacagctt tccccctcaa gtcaactcgc tgatcctgca aacgatcttg atttagtatt
     2221 tgatagtagg gtatggttcg aatagaccta ggagggccat aaaaatttaa atagtgtttc
     2281 tcatggccgg catagagttc agctagcgcc tgtaatttac cctttgtctg agtatcgatt
     2341 tcatcatttt ggtttagcgc attaatgaac tgatctttta atgctggttt ttcgataaac
     2401 tgcccacgaa gctcgtacat tagtgcttca atttcgatac ccttgtccag ggtggaaggc
     2461 agattcaatc cagcgacagg atcgatttct tgtagtatgt gagcaaactg atcatagaca
     2521 tcctgtgcaa aaatctgtaa caccactgca ggtaataaag gaattcctcc cgcatcctct
     2581 ttaaaagtcc agtgataagc cactactcct cgaggcaaaa caaaaggagc cgtagcttgt
     2641 gccgcatcgg aaatcattgg tagtacggat cgatgccatg ctccatgtaa ccctaccgat
     2701 tcactaaaat aatattgatg aggctcatct cgattcaagt ctttcgttga tagcgattca
     2761 gaattacaaa cttcaggttg ttcatccatt tgatagggaa aatcagcaat gtccctggaa
     2821 tcaactaatt cttcaacaag aataacgtta cctgcattct tgatcgcttg aactaaccta
     2881 tcattatctt caaaactact tccgcaccct ctaaacctta aatcaaatgc gatcacttta
     2941 acttcttctt caatgagacg atcaatcaat tcagcatgga gcgcacgagg ccatttactt
     3001 ggatcgattg tattattcgc aatttgatta ttatctttta gcttcgaagc tgagtattga
     3061 tcgatcgcga tcacaaccac atcagaagga actgaggtaa cgccgcgcag atggaaaaga
     3121 acattcaacc caaatttttc atcaagtgct aacccgaagg gtaatagata taaaacaact
     3181 ccaattagcc caataaaaaa tgagatcagc agctttttac cgtatccgta cttctgcaca
     3241 atgagagcaa atcacataga attagatggt aaaacactaa aatttacacc tgacaaacaa
     3301 cccacactgt tatatcatag ctacacaagt attaaaaagt ataagtagat attggatcta
     3361 gagagtagat ttaactaaat ttatagccaa actaggttat tgtattggaa gtatctggtg
     3421 ggtatttatt aacttcaata aattaaaagg agcaaaaaat gaaaaaattg atggcaattg
     3481 cactttcagc tatgttggtc gctggatcca cctttgtatt tgcaagtgga aataaagaat
     3541 ctaaacttga accacaacgc gcgcaagaaa tattgaatca aatgggctgt aaagacaaaa
     3601 aacctgaaga aacgatcaaa gatcgtacgg atggatcaat tgtaaaatgt ggtgatgtta
     3661 aaccgctagt aaaacgataa aataaattct tcttttaggc tattgcgagg taatgatcgt
     3721 ttcgcttgtg atagcctaga aagccggttc aattagactt ttaattcgtc atcttcaata
     3781 aaactttctt aatcattctc tattgatttc ttagcttagc acgtagtcga gcaattgctt
     3841 gggagtgaat ctggcatact cgtgactcac tcacaccgag tacctcccct atttcacgta
     3901 agttcatttc ttgctcgtag tgcatcccca tgacctcttt ttctctaatc ggtaacttat
     3961 cgattgccgc tactaaaagt tcacgtagat tcttatctac caatatttca agcggctcat
     4021 tcccagaatc tgagcataga cgatcaagaa agtgatcttc atcatcatcc tgaaaatcct
     4081 cataatagat tagctgcccg ccacgcgcat cttgaagtgc ttcatggtat tcttccaggg
     4141 atatatttaa ctctttagct atctcttgct ccgatggagg acagcctagc ttttgctcca
     4201 gtgaacgcat tgctacttcg atttgtcgca ttttttttcg cagactacgc ggtaaccaat
     4261 ctgattcacg taattcatcc agtattgatc cccggatccg ctgtgcggca tagctttcaa
     4321 actgccgccc ataagcacct tcgtaacgat taatcgcatc gagtaagccg atcattccag
     4381 cttgaatcag gtcatcgact tcaacactgg ctggtagctt catcatcata tgatgggcaa
     4441 ttcgtttcac gagtggagca aattcgacaa caaattgctc tttatcaata attccagaag
     4501 ttgtatacat aggctctaat tagactgaaa gttttacttt gcttaaatgg cttgttcgaa
     4561 tcaatcgata cataaaatta tcaacgccac cgctatattg attggaatag gaacatcgta
     4621 ataatttctc agccagccgc ttaaattcaa ttgcagcagt cgaagcagga aatgcatcga
     4681 ctaccgatcg acataattgg cttgcacgat gtaatttctc atcaaataaa atacaaccca
     4741 gatactcaag tcttacagac aaatgctgtt ttgcaacttt tgcgatattt tcatagatgc
     4801 tctgcgcatc aagctcagac ttaaccttat ttacgattac taagaaatcc tgccgcgcat
     4861 attcttggct catgatttta atcagtgtat aggcatctgt aatcgatttt gatgatccag
     4921 ataccgttat cagcacttgc tgcgaggcta ggctaagtgg caacacatga cccatctctc
     4981 caatcatggt atcgacgagc actacatcga tgggtaattc gcacaggcat ttaataatcc
     5041 actcttgctc ttcagctgac aattgagtca atgaatgaat gcctcgtgca gcagtaagta
     5101 ctaaaatatc atctggcgct tttgccagaa tttgatccag tgactgctca cgcttgatta
     5161 gatgggtcaa gtcataatga cttttgacac ctaaattagc actgacatta ttgtgatgtt
     5221 gattctcatc caggatgaga atgcgatttc caattttggc cagagcagca gcaagattga
     5281 ccacgatact cgttttgcca acacccattt tgccacctgt gaccgtaaac acttttactg
     5341 agtcacgtgc ctgcattgca gcgagctctc gcaaacccga tgcttggtca atcatttttc
     5401 tactcatagc tagcctcagc gtatcccttt ggttttgctg cactattccg gtcatagcct
     5461 gccattacta atgcaaattc tggatcctgc aaagaaaaag gggaattttc tgcgatgggt
     5521 ttgaaaattt tatgaagtaa atatcttgaa tttgcggcat gcagatcctc tggtactttc
     5581 tgtccattgg ttacgtaatg caacacgagc ttacgtcgaa taacaacatc aagggccgta
     5641 cctaaaccca ccgcctcatc aactttggta ataatacaac cctcaatccc attatattgg
     5701 taggcagaga tcacctcgtc taatgttcct ccattactcg tagcatttaa caagagcaag
     5761 tgcttaattt cagttccaca gtcccgcaat aaggccatct gttcgctaac catctgatca
     5821 cgctgactca taccaaccgt atcgatgagt acaatatgtt tactccgcag ctcaagtagg
     5881 gttaattgca aatcatcaat gtcttttata ctacgaactg gaattcccaa taacttgcca
     5941 taaattctca actgctcgtg tccaccaatc cggtagctat ccgtagtcag taatgcaact
     6001 ttatctgcgc catgcctaat gacagctctg gcagcaagtt tggcaatcgt cgttgttttt
     6061 ccaaccccag taggaccaat cagtgcataa acaccgccat tctctatcag ttgatcactc
     6121 gttgcagtgc gtaaattgag cgttagtagc gcaaccgtct gctttaaact ctcttcgtaa
     6181 ttactaactt tggttagctt ctcaagtaaa tggcgcgata ataatggact aaaccctgca
     6241 tcaagcagtg ttcgcatcag tttcatcgtt tccggtttac tttgtgacaa acttccccaa
     6301 gcaactgaat tcagttgatc ttccaatgta tggcgcattg cctgaatttc attaatgata
     6361 ttcagtgcaa gcgcttcaga aatatttgat tctttcttaa cttgttgaat cgcttctagc
     6421 acttcaatat ttgggttaat aaccgataat acttcctctt tcttttcgac ggggggcggt
     6481 gttggaacaa acttagattc gggactattg accaagcttg acatttcatt acttgccaat
     6541 gcaacaattt caatgccttc atcgacttgt ttattcgaca agataactgc atcagcaccc
     6601 agttcttcct taacattgcg taacgcctcg tgtgaagttt tcgcaataaa tcttctcatt
     6661 ttcatttatt ccctccaacg atcgatacaa atttaatttt tctagaatcc actacctcag
     6721 catgtgaaat cactttgagt tgtggtaatg ggcggcgcaa gaaacgcgcc aataataatc
     6781 ggattgaatc aggcactagc aaaactgcgg gaattccaag ctgttcatgt tgatcgacgg
     6841 ctttactgac ttccttcaat aacgtatcag ccaagcctgg ctctattcca gcacccgcaa
     6901 caagccctga ttgtgcaact tgtactaaaa tgctttctaa attgtgatga agactcatca
     6961 cttgtaattc ttcacctgaa gggaaaaact gctccagaat ggaacgccct agtgcaactc
     7021 gcacaagcgc tgttaactca ttggtatctt gagtacgggt agcatgttct gtgagtacat
     7081 caacaatggt acgaacatcc cgtatatgaa cgccttcttc taataaattt tgcaaaatct
     7141 tctgaaccgt tgctaaaggt aatagcttcg gtattaagtc ttcggttagt ttaggtatac
     7201 tgcgacccaa gtgatcaaag agtttctgta gttcttctct acccagtagc tctgctgcat
     7261 aggagcgcat taaatggttt acatgggtgg taaccacggt actcggatca acgacggtat
     7321 aaccatgtgc ctgagcatca tcttttaagg caggttcaat ccatacagca ggcaatccaa
     7381 aagccggatc actggtattc acaccagata atgtcttcgt cgcttgaccg ggattaatcg
     7441 ccagatacat tccgttataa gattctcctt gcccaatgac agaacctttc agcgtaattc
     7501 ggtaagcatt aggtcttagc tctaggttat ctcgaatatg aacaacaggt ggcaagaacc
     7561 ccacttcttg cgcaaatttt ttgcgaatac cattaattcg ctttaataga tcaccttgtt
     7621 gtgttttatc gaccaatgga atcaaccgat aaccaacttc caaacccacc atatcgacag
     7681 gcatcacatc attccaacta gcttctcgcc cctcagtttc ggcaactggg gatgaggtag
     7741 cctcttcagt aactgcttct ggttccggta gatagttaac tttaaaataa gcgataccgg
     7801 ctatgatgga tgctagcaat aaaaagacaa aattaggcat acccggaatc aaacccatca
     7861 cacccaagat acctgcagtt agaaataata cttgtggctt attaaataac tgactgacaa
     7921 cttgctgacc taaatcctga tcagaactca cgcggctgac caccataccg gctgctgttg
     7981 aaataatcaa tgaaggtatt tgcgcaacca atccatcacc aatcgttaat atcgtgtaat
     8041 actttgcggc tgcactcaca tcaaggtcat gctgaaatac cccgacaacc aat
//
LOCUS       DAOQKQ010000119         8062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_4355_length_8062_cov_6.530161, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000119 DAOQKQ010000000
VERSION     DAOQKQ010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 8062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8062
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4355_length_8062_cov_6.530161"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1211)
                     /locus_tag="PKZ49_08635"
     CDS             complement(<1..1211)
                     /locus_tag="PKZ49_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36666.1"
                     /translation="MASYSTSLSVHKVLHLAQMQVSKPSDQTVTLMVCVCSFDANAST
                     NFSRTRYMLRFLMNPSYSGGSFGASTSWRDVTPYYIDKVYTNTLLTKPDAANDKRYTF
                     PTNADEYYFAEDLVGAYIYVEDASFATPNIHIAEGDKITSSDHTWHDFTSATMSVNTE
                     SNHPGSFSTWGGVTMDVLRIHKNLETGKIPYDQLCSDTASIFDQYTFSSEIKNERIIL
                     SMHTGDSGRANMTLGFNYVSHTHFSTGSIKGLADYIYNAASDVMYDKRLVYGFPNYDN
                     INKSNVDTVFDGKTIQRWNSGTLPSAAASGTATVSTNGYQKIAGQITLGSGFVAANLT
                     NNLYKILSCDVSIVTDDFIISEFDVHGRYGGFTDFQARSNRCSGNIQFMPAGSSLLSH
                     GSNFSEYEGNT"
     gene            complement(1215..1436)
                     /locus_tag="PKZ49_08640"
     CDS             complement(1215..1436)
                     /locus_tag="PKZ49_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36667.1"
                     /translation="MLRIGTDENHWGGLFDETDGFDKPGTFSALDNFIPNHKSYSLVR
                     RPGFSKLLDKPTTSNGGLGSHTGSMGGKG"
     gene            complement(1436..2968)
                     /locus_tag="PKZ49_08645"
     CDS             complement(1436..2968)
                     /locus_tag="PKZ49_08645"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HND36668.1"
                     /translation="MMSEQDVSDCSFDAIQELQTNSLCSRMEVKKLIDVNGEISTVDI
                     LDGGSGYLFPPTVTITDNNDDDCLSFDGNDYGSFTTKNIGTTHTIEFWLDASNKDFLI
                     CSRTGGTGGVEFDSTDKIYYSGDSTSQASKSITALSPGTKHHFAITRNDSTTITFYQN
                     GVSLGTSTISSNTALSLSAIGYRAHGASLYYVGKLGLFRIYKSALTAEQIKRQYNGGY
                     GNSPLDIDSYLTYEFDGTGGTGSTETDQSTDGGSTLTINGATRSTFGNSYLSSTEKAK
                     ATAFIEKGIVTKIVITNQGRGYIPKPIVTISGGGGGTGAIAVAEIATTTYDKIITSKF
                     GGSTTGDIDVECLYVDSMYRVGLDGTKNPIKITNRQSIQDERDNGANYNDASDYTAAI
                     EWNKNNIHIFTPWDMTGSTIELWIRFQIPYVQDANVGTNADSYLDTIPVQFQNRLVSG
                     VKYHIYESMEEMSGFSGKYKPMMDIFGNQWFNRDLPYVKQEANHARTSADVHIIKPSG
                     VF"
     gene            complement(3019..3765)
                     /locus_tag="PKZ49_08650"
     CDS             complement(3019..3765)
                     /locus_tag="PKZ49_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36669.1"
                     /translation="MASPIFDSLNQGLSIQLDDYVASATTDGKTYTSADRSDFLNRAS
                     KTLFNFFYDQFKEMVYSSQTPINRDDNLTVALNRFRDFIAVAAPTSTAGSLIALSGLT
                     RFLEVLNVVNTISTLQCRIVDKSQVPFVFTNHQRFKPTAANPICYVGDATNITVLPNS
                     SDNQSKLFSVTYLKYPDFTCVANDSSEKIEWNATFYEDLKYLAVGFARLDNGEYQAFE
                     SIKQAVFTMYKGNTQAFYDNKAVRRSVDNQ"
     gene            complement(3776..4387)
                     /locus_tag="PKZ49_08655"
     CDS             complement(3776..4387)
                     /locus_tag="PKZ49_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36670.1"
                     /translation="MATQTKSQAGLVSKSAKRTLSDTRGAKSRKGDNPTRHDTFFIRA
                     GQVNASFASGANTFVHLLTVGPEAFANNSSGTSKLINFRVSFIGGANNLDFTNLKVQK
                     VAFNSGGNSYGAADSTSGAGNNSVAVAPATAFYDQAQNAADHRYTGGSGSVVQVIRNV
                     DSTDTQLEVKPCDVIMLTLTMTGFTGTVTGAYDIALEFTELSI"
     gene            complement(4450..4755)
                     /locus_tag="PKZ49_08660"
     CDS             complement(4450..4755)
                     /locus_tag="PKZ49_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36671.1"
                     /translation="MAYSGSVAKAYALVPATQYIGPEALLMEVGTSTLATGTVEVPTK
                     LTSIQFAYLSYDGAVTSGGETEALTTDKGITSSAVTVTGKGNLTFSYIFIGRAAAQL"
     gene            complement(4785..>5045)
                     /locus_tag="PKZ49_08665"
     CDS             complement(4785..>5045)
                     /locus_tag="PKZ49_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36672.1"
                     /translation="VNFVYSREMSDSTRYRNSFLFANFDKIEHLVLKKKDGGNFDTYI
                     DKGKNGTGLGDSEKTTKYQWRSIDGLGFNDTDAAAFEAYFPA"
     assembly_gap    5046..5145
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5146..5858)
                     /locus_tag="PKZ49_08670"
     CDS             complement(<5146..5858)
                     /locus_tag="PKZ49_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36673.1"
                     /translation="MAGSSGEAIRGAVSLADEYTVLRKNDIDKAGLPILRLRALVPFQ
                     RIMMYERPKIKLVGDTKVSNFEQAEEINTVAVTGSTSGATMTVAIANATAAQLVVNQY
                     LINENINYSATAGYTKPANIQTVTSPEAAKILSIGEPGATNTDVVLLRMHQWTGGAST
                     NVGAAGGTWAAADNIRRSARSTFDGANSGTAIRINPEIRTNYLQIFEKPFEVTELQQN
                     VEKYSIDNPIEYQQYLAMMR"
     gene            complement(5872..6819)
                     /locus_tag="PKZ49_08675"
     CDS             complement(5872..6819)
                     /locus_tag="PKZ49_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36674.1"
                     /translation="MSSWGEAIAEVNKVAEDEGRSEVVKTTDQIKTVEDQGEDVKPEN
                     VEIEESSKPEVNNNGSTKPKAPNDVKPDGSEISDEVRAKIVSEIEAKYADYENLKKTS
                     EEYIALKAQVDAFEADPKSFIEKNFPQFKYQATEKTIDEKIRELKKKFGPDFVYDPSE
                     ARDPDSASFKYDYEYDRIKQEHMQEEVSRKFEIQKSMEENRKRIQESLGSVARSIEKV
                     KSDYGLKDDVMNKFVQEHMNIKDDPIKQFESWLLLKVKAEYGLNKVAERMKAQDQIEK
                     EKADALKSTPVSGKSASKSRSDDDRDFDFLFTKQTRSGY"
     gene            complement(6816..>8062)
                     /locus_tag="PKZ49_08680"
     CDS             complement(6816..>8062)
                     /locus_tag="PKZ49_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36675.1"
                     /translation="MQEGGVPVGAVEDLESIYTALNQTVLLTLLNARLQCNFHIMAPT
                     GSIKDMDAFELQWSKTGSVVEVDPVSVGGSQPMMPVPVYAQQLPSTFGDLTQMLIKMA
                     EYSSSVSEALQGINTGEKPSGVALEQLQSYGGLKFRPHAETNQTSLSRLGKVAFDFAK
                     QLMPNDVIIRFKEEDDDFLESISITDDEKQDPGLLAQRVKDEKSAIAQRVNMPGYNVE
                     MPLNYRVQVGEDVVFVNDFKNSETDLDFRIVAAPSTESQRYNWQAVIGNMIRQFPEMT
                     PVLGPEALKMLDYPNADKLFKKMDVLEQTRAQLRDALSQIEILKSEQVNLMQQNIRTK
                     MSADVTKVTADYKMKLEKMLNDMKLELASIESSGQDVDGNGEKIRQQIQSLEAFMASQ
                     MQDVSPQGSANSVEQTLPEGSL"
BASE COUNT         2315 a         1817 c         1436 g         2394 t
ORIGIN      
        1 aaagtgttcc cctcatattc cgaaaaatta gagccgtgcg aaagaagcga cgatccggct
       61 ggcataaatt gaatattgcc ggaacacctg ttacttcttg cttgaaaatc tgtaaatcca
      121 ccatacctgc cgtgaacatc aaactcggaa attataaaat catcagtaac aatagatacg
      181 tcgcacgata atattttata taaattatta gtcaaatttg cagctacaaa tcctgagccc
      241 aaagttattt gacctgctat tttttggtat ccattagttg ataccgttgc agttccacta
      301 gcggcggcag acggcaatgt tccagagttc cacctttgaa tggttttacc atcaaaaaca
      361 gtgtcaacat ttgatttatt tatattgtcg taattaggga agccgtaaac aaggcgtttg
      421 tcatacatca catcagatgc ggcattatat atgtaatcag ccagcccttt tatgctaccg
      481 gtagaaaaat gagtatgact aacataatta aacccaagcg tcatattcgc tctaccacta
      541 tcgcctgtgt gcatggacaa tattatacgt tcattcttta tttctgaact aaaagtatat
      601 tggtcgaaaa tagaggcggt atcggagcat aattggtcat acggtatttt gcctgtctct
      661 agatttttat ggatacgcaa cacatccata gtaacgccac cccaagtact aaatgaaccc
      721 ggatgattac tttccgtatt aacactcatc gtagcactag tgaagtcatg ccaagtatga
      781 tcgctagaag ttattttatc gccttcggca atatgtatat ttggcgtcgc aaaactagcg
      841 tcttctacgt atatgtatgc accgactaaa tcttcggcga aataatactc gtctgcattc
      901 gtagggaatg tatatctctt atcattagcc gcgtccggct ttgtcaataa tgtgttggta
      961 taaactttat caatgtaata cggagtgaca tcacgccaag acgttgatgc cccaaatgat
     1021 ccaccagaat aagatggatt cattaaaaac ctaagcatgt atcttgttct gctaaaatta
     1081 gtagaagcat tagcatcaaa agaacacacg caaaccatca atgtaacagt ttgatccgac
     1141 ggcttactaa cttgcatttg agctaaatgc aataccttat gtacagatag actagttgag
     1201 tatgatgcca ttatttatcc tttacccccc atactccccg tatgagaacc tagacctcca
     1261 ttggaggtcg ttggcttgtc tagtaattta ctgaacccag gtcgccttac gagtgaataa
     1321 gacttatggt ttggtataaa attatctaaa gccgaaaatg ttccaggctt atcaaaccca
     1381 tcggtttcat caaataggcc gccccaatga ttttcgtctg tcccaattct tagcattaaa
     1441 acacacccga tggtttaatg atgtgaacat cagcggatgt ccttgcgtga ttagcttctt
     1501 gcttaacgta aggcagatca cgattgaacc attgattgcc aaatatatcc atcattggct
     1561 tatacttacc agaaaatcca gacatttcct ccattgactc gtagatgtga tatttcacac
     1621 ccgatacaag cctattctga aattgaaccg gaatagtatc taaataagaa tctgcgttag
     1681 ttccaacatt cgcatcttga acataaggta tctgaaatct tatccaaagc tctatagttg
     1741 accccgtcat atcccaaggc gtaaatatat gaatattgtt tttattccat tcaatagccg
     1801 cagtataatc ggatgcgtca ttataatttg caccattatc tctttcgtct tgaatagact
     1861 gacgatttgt tatttttata ggattcttgg tgccatcaag cccaactctg tacatcgaat
     1921 caacatacaa acactcaaca tctatgtcgc cggtggttga ccctccgaat ttagaagtta
     1981 ttattttgtc gtatgttgta gttgctatct cagctacagc tatagctcca gtaccgccgc
     2041 ctccaccact tattgtaact ataggctttg gaatgtaacc acgaccttga ttagtaatca
     2101 ctatcttagt tactatgcct ttttctatga aagccgtagc tttagctttt tctgtagaag
     2161 ataagtaact atttccaaaa gttgaccttg ttgcgccatt tatagtcaac gtactaccac
     2221 catcagtact ttggtcggtt tctgttgaac cagtaccacc agtaccatca aattcatacg
     2281 taagatatga atctatgtct aaagggctat taccatatcc gccgttatat tgccgcttta
     2341 tttgctccgc cgttaatgct gatttataaa tgcggaataa accaagcttc ccgacatagt
     2401 aaaggctcgc tccatgagcg cgataaccta tcgcgctcaa agacaatgct gtattagaac
     2461 ttatcgttga cgttccgaga gacacgccat tttgataaaa agtaattgta gtgctatcat
     2521 tccgtgtgat tgcgaagtgg tgctttgttc caggacttaa tgctgtaata gattttgaag
     2581 cttgagatgt agaatctcca gaataataga ttttgtctgt actgtcaaac tccacgccgc
     2641 ccgttccgcc tgttcgcgaa caaattagaa aatctttatt cgacgcatca agccaaaact
     2701 ctatcgtgtg cgtcgttccg atgtttttag tggtaaacga accataatca ttaccgtcaa
     2761 aactaagaca atcatcatca ttattgtctg ttattgtaac cgtcggagga aataaataac
     2821 cacttcctcc atctaatata tctacggttg atatttcacc attaacatca atgagctttt
     2881 tcacctccat acgagaacac agcgaattag tttgtaactc ttgtatggcg tcaaaagaac
     2941 aatcagagac gtcttgctca gacatcatac tcgcgtcttt gatgttcttt aatgattttc
     3001 gtcttagatc actaaatgtc attgattatc aacactgcgt ctaacggctt tattatcgta
     3061 aaacgcctgg gtgttgcctt tatacattgt aaatacagct tgtttgatag attcaaaagc
     3121 ttgatactca ccattatcta aacgagcaaa tccaacagct aaatatttta agtcctcata
     3181 aaaagtagca ttccactcta tcttctctga actatcatta gctacacaag taaagtcagg
     3241 atatttcaaa taagtcacgg aaaataattt agattggttg tcagagctat tcggtaatac
     3301 cgtaatatta gtggcgtcac caacataaca tataggatta gcagccgtcg gtttaaatcg
     3361 ttggtgattt gtgaaaacaa atggaacctg agatttatca acgatcctac attgtaacgt
     3421 agagattgta ttgaccacat ttaaaacctc aaggaatcta gtcaatccag acaaggctat
     3481 aagcgacccc gcagtagaag tcggagcggc gacagcgata aaatcacgga atctattcaa
     3541 agccaccgtg agattatcat ctctgtttat aggagtttga gacgaatata ccatttcctt
     3601 gaattgatcg taaaagaaat tgaacagggt ttttgaggcc ctgttcaaaa agtctgaccg
     3661 atccgcagat gtataagtct taccgtctgt cgtagccgat gcaacatagt catcaagctg
     3721 aattgacaac ccctgattta gagaatcaaa aataggagaa gccatttata tatccttaga
     3781 ttgaaagttc tgtaaattct aacgcaatat cataagcacc agtaaccgtt ccagtaaacc
     3841 cagtcatagt aagcgttagc ataataacat cgcaaggctt cacctctaac tgagtgtccg
     3901 ttgaatcaac attacgaatt acctgcacca cgctaccaga gccaccagta tatcggtgat
     3961 ctgcggcgtt ttgcgcttga tcgtagaaag ccgtggccgg agctacagcg acgctattat
     4021 tgccagcgcc agaagttgag tcagcggccc cgtaactatt tccacctgag ttaaaagcta
     4081 ctttctgaac ttttaaatta gtgaaatcta agttattggc gccacctata aaagaaactc
     4141 ggaagttgat aagcttcgat gttccacttg agttgtttgc aaaagcctca ggaccaaccg
     4201 taagcagatg aacaaacgta ttagcgccag acgcaaacga ggcatttact tgtcctgcgc
     4261 gaataaaaaa agtatcgtgc cgagtaggat tatcaccctt acgcgactta gcaccgcgtg
     4321 tatcggataa tgtccgtttc gccgatttag atactaaacc agcttgtgat ttggtttgag
     4381 ttgccatttc atttctccat tgttagggtt ttgggggcta tatctcaagc ccccattacc
     4441 aaattattat taaagctgcg cggctgcacg acctatgaaa atatatgaaa acgtcaaatt
     4501 acctttacca gtaaccgtta ctgcgctgga tgtaataccc ttatctgtag taagagcctc
     4561 agtttctcca ccagacgtta ccgcgccatc ataagacaga tacgcaaatt gaattgacgt
     4621 taatttcgta gggacctcta ccgtaccagt agcaagagtg ctagtcccaa cctccatcag
     4681 aagagcctcc ggtccaatat attgcgtcgc agggactaaa gcataggctt tagctacact
     4741 acctgaatat gccatataaa atctccttaa ttataattcg ttaattacgc aggaaaataa
     4801 gcttcaaaag ccgcagcatc cgtatcattg aagcctaagc cgtcaataga acgccactga
     4861 tattttgtag ttttttccga atcgccaaga ccagttccat ttttaccctt gtcaatatag
     4921 gtatcgaagt taccgccgtc ttttttcttg agtaccaagt gttcgatttt gtcgaaatta
     4981 gcaaacaaga agctattgcg gtaacgagtc gaatccgaca tttctcggct ataaacaaaa
     5041 ttaacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncgcat catcgccaaa
     5161 tactgttgat attcaatcgg gttatcaatc gaatattttt ctacattctg ctgcaattcc
     5221 gtaacctcaa aaggcttctc gaaaatctgc aagtagttcg tgcgaatttc gggattgata
     5281 cggatcgctg ttcccgaatt agcaccatca aaagtagaac gcgccgaacg acggatatta
     5341 tccgcagccg cccaagttcc accagccgca cctacgttag tagaggcgcc accagtccat
     5401 tggtgcatac gcagtaacac gacatcggta ttagtagcac cgggttctcc aatactcaaa
     5461 atcttagctg cttccggaga agtaaccgtc tgaatattgg cgggttttgt atagccagcc
     5521 gtagcgctat agttaatgtt ttcgttaatt aaatactgat tgacaacaag ttgtgccgcc
     5581 gtagcattag caatagcaac agtcattgta gcccctgacg tggagccagt taccgctacc
     5641 gtgttaattt cttcggcttg ctcaaaatta cttaccttgg tatcgcctac taatttgatc
     5701 ttgggacgct catacatcat aatgcgttga aacggcacaa gagcgcgtaa gcgaagaata
     5761 ggcaacccag ctttgtcaat gtcattcttt cgtaataccg tgtactcatc agcgagtgac
     5821 actgcaccgc gaattgcttc accagaactt ccagccataa tttaaactcc tttaatatcc
     5881 acttctagtc tgttttgtga acagaaaatc gaaatcacga tcatcatctg atcttgactt
     5941 actcgcagac ttcccggata caggagttga ctttaatgcg tcagcctttt ccttctcaat
     6001 ctggtcttga gccttcattc gctctgctac tttatttaat ccatactcag cctttacctt
     6061 gagtaaaagc caactttcaa actgcttaat agggtcatcc ttgatgttca tgtgttcctg
     6121 aacgaactta ttcatcacat catctttcaa cccataatca gatttaactt tttctataga
     6181 tcgcgccacc gatccaagag attcttggat gcgcttacga ttttcctcca tagatttttg
     6241 tatctcaaac ttacgagaga cttcttcctg catgtgttct tgcttgatac gatcatactc
     6301 ataatcatac ttgaaagaag ctgaatcagg atcacgcgct tctgacggat catatacgaa
     6361 atcaggacca aacttcttct ttaattcacg gattttttca tcaatagtct tttcggttgc
     6421 ctgatatttg aattgaggaa aattcttctc aataaacgat ttagggtcag cttcaaaagc
     6481 atcaacttga gcctttaatg caatatattc ttcgctagtc tttttaagat tctcataatc
     6541 tgcgtattta gcttcaatct cagaaactat tttagcgcga acttcatctg aaatttctga
     6601 gccatccggc ttaacatcat ttggcgcctt cggcttcgtc gagccattat tgttaacttc
     6661 tggtttagac gattcctcaa tctctacatt ttcaggttta acatcttccc cctgatcttc
     6721 aaccgtttta atttgatctg tagtttttac cacctcagaa cgaccctcat cttccgctac
     6781 tttattcact tctgcgattg cctcgcccca tgaactcata aacttccttc cggtaatgtt
     6841 tgctctactg aattggcaga cccctgagga ctaacatcct gcatttgcga cgccataaac
     6901 gcttcgagcg attgtatttg ctgtcttatt ttttctccat tgccatcaac gtcttgacca
     6961 gaagactcta tagacgccag ctcaagcttc atgtcattaa gcattttctc aagcttcatc
     7021 ttgtagtctg ccgttacctt agtgacatca gccgacatct tcgttctaat attctgctgc
     7081 atgagattga cttgttcaga cttcaatatt tcaatctgag acaaagcatc gcggagttgt
     7141 gcacgagtct gctcaagcac atccattttc ttaaataatt tatctgcatt aggataatca
     7201 agcattttaa gcgcttctgg tccaagtacc ggcgtcatct cagggaactg gcgaatcata
     7261 ttaccaataa cagcttgcca attataacgc tgagactcag ttgacggagc cgctacaata
     7321 cggaaatcta ggtctgtttc gctattctta aagtcattaa caaaaacaac gtcctcacca
     7381 acctgaaccc tatagttaag tggcatctcc acattatagc ccggcatatt aacacgttga
     7441 gctatagcac tcttctcgtc ttttacacgt tgtgcgagta atccgggatc ttgcttttca
     7501 tcatccgtta tactaattga ctccaaaaaa tcatcatctt cttctttgaa tcgtataatg
     7561 acatcattag gcattagctg tttagcaaaa tcaaaagcga ccttgccaag cctagacaac
     7621 gaagtctgat tagtttcagc atgaggacgg aacttcaatc cgccataaga ttgtaactgc
     7681 tcaagcgcaa cgccagaagg cttttcaccg gtatttatac cctgcaatgc ctcagataca
     7741 gatgaagaat actcggccat ctttataagc atttgtgtga gatcgccaaa agtagatggt
     7801 aattgctgcg cataaacagg aacaggcatc atcggctgac ttccaccaac agacactgga
     7861 tcaacctcaa cgactgagcc cgttttactc cactgcaatt caaaggcatc catgtcttta
     7921 atagaaccag ttggagccat aatatgaaaa ttacattgca atctagcatt aagaagagtc
     7981 aggaggacag tttgatttaa tgctgtgtaa atagattcca agtcctcgac ggcacctaca
     8041 gggacaccgc cctcctgcat gt
//
LOCUS       DAOQKQ010000120         7979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_4406_length_7979_cov_5.070924, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000120 DAOQKQ010000000
VERSION     DAOQKQ010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 7979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7979
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4406_length_7979_cov_5.070924"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..407)
                     /locus_tag="PKZ49_08685"
     CDS             complement(42..407)
                     /locus_tag="PKZ49_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36676.1"
                     /translation="MSQFVIAFLGLFFITTLTYSQESADEKRLDEIAERGAHVMPFDL
                     EKTLHIFSKTNHGGIQQVIVKDKSDTEQINLIRSHLSKIAAEFKRSNFSNPEKIHGKN
                     MPGLADLKTAKDGQIKICL"
     gene            complement(505..1716)
                     /gene="purT"
                     /locus_tag="PKZ49_08690"
     CDS             complement(505..1716)
                     /gene="purT"
                     /locus_tag="PKZ49_08690"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HND36677.1"
                     /translation="MNKRLTIGTPLSAAATKVMLLGSGELGKEVIISLQRLGVETIAV
                     DRYANAPGHQVAHRAHVINMTDPMALRSLIEQERPAIIVPEIEAIATEVLLEIEKEGL
                     ATVIPTARAARLTMNREGIRSLAAETLGMPTSPYAFANSLAALRAAIDEQIGYPCIVK
                     PVMSSSGKGQSRIENVDGIEAAWNYAASGGRVDQGRVIVEGVIDFDYEITQLTVRAMN
                     DRAEVQTYFCEPIGHVQVNGDYAESWQPQAMTAIAMERARELAKKITDNLGGLGIFGV
                     ELFVKGDEVWFSEVSPRPHDTGMVTMCSQKQSEFDLHARAILGLPVDTTLSAPGASAV
                     IYGQLEEKGIAFTGLGEALSVPQSDIRLFGKPESFKKRRMGVALANGANTDEARTRAK
                     LAASRVCPIKS"
     gene            complement(1769..5083)
                     /locus_tag="PKZ49_08695"
     CDS             complement(1769..5083)
                     /locus_tag="PKZ49_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HND36678.1"
                     /translation="MIRLLSIVVFFICFIATSVLGQAIDLNDQLQSIQTKLEALNSSD
                     ETVQKKSLKEIFLKTQQVLLDHQSYLAKTQYYTEQLAQFPQQLENLEQQKTNVNSTQP
                     NFFELKQLTLSEMAQRNIETQAKLSEFQNQQQKILSELATLREQVVTNRNELATANST
                     RNIESAEIFAELDLSNEKIVEALRMYEMLQSQALADKMRMLELEALALPKAIEMATFQ
                     EEFILRPEIQQLIEESEWLAVEINLKRKAETEVAVEKSGLVLEESEWKHPILLIIVKK
                     NELLATELSHYVEQSSKLIVQKARSENRLNLIINNYAALQQRLALYEDDEALGSEIRK
                     QFKYVLTKPNTQLTQKKLNNAKLELFQLEQEKLQMQDDKTYLKRMTKGYTINPDAAVY
                     PDIMRAFQELKMFRLQVIDKFIEILREYLKELEQYASLQHQLLEKIDQYENLLKENLL
                     LTRNAKPIYQVTTNDIDKAMTWFLTDKTQSAFSKLVDRVGLVSFIVTILSFILLSVFW
                     WFLWPRYLMWEKVGKEAWGKVKQDKIIYSVRLLIFIFSQSLLFASPFFLIYLALEHSV
                     NTEINRAFSQVFYVAAIAVVFWNVLYQISRPSGLLNQHFKFPVDFVAQLHSSIRHYGP
                     VIVILCLITAFTDAIGEDMVRHVVGRVSFITVCFLLALLSLDWMGVTKVGKALYRDRH
                     FNYLKNPKLWAWIFFLQLLYLIATAAIGYYFAALYQAILIFETIAWITICGLIFLQAY
                     RGLLIAQRRITYKRAIEKREEMIALRTSIAGKAEVELVDENYMDVETISKQSHTLLTI
                     SVWGLLLLGLSSIWVDLLPTLGFLDKIVIWRTSAISEGETIVRLITLETLLVAMIILV
                     LAFIAAHNLPGTLEILVLRHLSLDTGTGYAITTLLKYVIIIIGIAIMSQKLGVEWSNI
                     HWLVAALGVGVGFGLQEIVANFVSGLILLFERPIRIGDTVTLSDVTGTVSKIHIRATT
                     LIDSDRKEVIVPNKTFITQQFINWTLSDEVTRIKIPVGIAYGSDCDNARKVLLEIAHN
                     HPLVLREPEPLAVFVDFGASTLNFELRVYAGQFSDRLDLVNDLNMEINRRFLAEGIEI
                     AFPQLDVHLHRASTKK"
     gene            complement(5162..5944)
                     /gene="tatC"
                     /locus_tag="PKZ49_08700"
     CDS             complement(5162..5944)
                     /gene="tatC"
                     /locus_tag="PKZ49_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HND36679.1"
                     /translation="MDEEGSFISHLVELRTRLIRIITVLVVGFIPCAYFARELYTLLA
                     QPLLEKLPQGGQMIATAVATPFFVPMKVALMTAFLLTLPHTLYQIWAFVAPGLYAHEK
                     RLFLPLVVASSGLFFIGMAFAYFAALPLVFEFIIYFAPQGVAVMTDIGEYLGFVMTMF
                     LTFGITFEVPVFVVVLARTGIISLTKMKEVRQYVLVGAFVIGAIFTPPDVISQFLLAI
                     PLYLLYELGLFLAGMSIKKGFSASNELATKQENKPSASSNEK"
     gene            complement(6028..6417)
                     /gene="tatB"
                     /locus_tag="PKZ49_08705"
     CDS             complement(6028..6417)
                     /gene="tatB"
                     /locus_tag="PKZ49_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HND36680.1"
                     /translation="MFDISFAELLVISVVALVVIGPERLPRVARTLGFLLGRARRYVE
                     TVKNDIRNEIELDELRRIKDTVQDTAQSIEKSVKQEIQQIQASVQPTASDSHDKKIKD
                     TSPVNNNHAGHEATPIASQSISNSKGD"
     gene            complement(6537..6776)
                     /gene="tatA"
                     /locus_tag="PKZ49_08710"
     CDS             complement(6537..6776)
                     /gene="tatA"
                     /locus_tag="PKZ49_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HND36681.1"
                     /translation="MGTFSIWHWLVVLAIVVLVFGTKKLRNLGSDLGNAVKGFKEGMK
                     GSEGEPMESPANTQQQIANRSTEDVSIKEKNETKL"
     gene            complement(6801..7154)
                     /locus_tag="PKZ49_08715"
     CDS             complement(6801..7154)
                     /locus_tag="PKZ49_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HND36682.1"
                     /translation="MSDCIFCKIVRKEIPAKIIYEDQHCLAFHDINPAAPVHFLIIPK
                     LHIASLYEVDDTHQLILGKMLGLVPKLAKQEGCEHGFRTIINTGKVGGQEVFHLHIHV
                     IGSKNVLPAMIHRSQ"
     gene            complement(7155..7484)
                     /locus_tag="PKZ49_08720"
     CDS             complement(7155..7484)
                     /locus_tag="PKZ49_08720"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HND36683.1"
                     /translation="MSHASILHHLATVIDDRRTADPSTSYVAKLLNSGQDKILKKVAE
                     EAAETIMASKDGDTTHVVYEVADLWFHCLILLAHHGLRPEDVLAELARREGLSGIEEK
                     RLRNTEK"
     gene            complement(7485..7871)
                     /gene="hisI"
                     /locus_tag="PKZ49_08725"
     CDS             complement(7485..7871)
                     /gene="hisI"
                     /locus_tag="PKZ49_08725"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HND36684.1"
                     /translation="MPASWVDKINWTTDGLVPVIAQEEATGTVLMFAWMNKEAVKLTV
                     ETKVVVYWSRSKQRLWRKGEESGHTQKVKAIYLDCDDDVLLIQVEQMGGIACHTGRKS
                     CFFNKLEGDQWVVNAPIIKDPSEIYQ"
BASE COUNT         2313 a         1810 c         1432 g         2424 t
ORIGIN      
        1 aatgcctgtt tgagtcacac ccagattaaa ctgaaaatag tttagaggca tatttttatt
       61 tgaccatctt tggcagtttt caaatcagca agcccaggca tattcttgcc atgaatcttt
      121 tctggattag aaaaattact tcgtttaaat tctgctgcaa ttttagataa gtgcgatcga
      181 atcaaattaa tttgctcagt atcagattta tctttcacaa taacctgctg aatccctcca
      241 tgattcgttt tagaaaaaat atgtagtgtt ttctctagat caaatggcat gacgtgcgca
      301 ccacgctctg caatttcatc gagccgcttt tcatctgctg actcttgact gtaggttagg
      361 gttgtaatga aaaataatcc cagaaaagca ataacgaatt ggctcatttt agtctctcct
      421 tattaagaga tctctttaga attcatatca actttttcgc aagtgcaatt tttctggaaa
      481 taaagctgaa acaagccgta attcttagct tttaatcggg caaactcggc tcgctgccaa
      541 tttagcacgg gttcgcgcct catcggtgtt ggcaccgttg gctaacgcaa cccccatccg
      601 tcgtttctta aaggattctg gcttaccaaa taaccgtata tcgctttgtg gaacactcaa
      661 tgcttctcca aggcctgtaa aagctatacc tttctcttcc agttgaccat aaatcactgc
      721 gctcgcgccg ggtgctgaaa gtgtcgtatc aactggcaaa cccaagattg ccctcgcatg
      781 taaatcaaac tcgctttgct tctgactaca catcgtcacc atccccgtat catgagggcg
      841 aggactaact tcgctaaacc atacctcatc acctttaaca aacagttcta ccccaaaaat
      901 accgaggcct cccaaattat ctgtaatttt cttagccaat tcacgggctc gctccatcgc
      961 aatcgctgtc atggcctgag gctgccaact ttcagcataa tccccattca cttggacatg
     1021 accaatcggt tcacaaaaat aggtttgtac ttcagcacga tcattcattg ctcgcacggt
     1081 taattgtgtt atttcgtaat caaaatcaat gaccccttcc acaatgacac gtccctgatc
     1141 gactcgtccg ccgctagctg cataattcca agccgcttca attccatcca cattttcgat
     1201 tcgtgattgc cccttacccg aagaagacat aaccggttta acgatacagg ggtaaccaat
     1261 ttgttcatcg attgcagctc gcaacgccgc taggctattt gcaaatgcat aaggtgaagt
     1321 tggcatgcct aacgtttctg cagctaaact acgaatacct tcacgattca tagtcaatcg
     1381 tgctgcgcgt gccgttggaa taaccgttgc aagcccttcc ttttcaatct ctagtaatac
     1441 ttccgtcgca attgcctcga tttcgggtac gatgatagcg ggtcgctctt gctcaattaa
     1501 actgcgtaat gccattgggt cagtcatatt gatgacatgt gcacgatgcg caacttgatg
     1561 gccgggtgca tttgcataac gatcgacagc aatggtttca acgcccaacc gttgtaatga
     1621 aataatgact tccttcccaa gctcaccgct acccaataac attactttcg ttgctgctgc
     1681 actgagtggt gtgccaatcg tcaatcgttt attcatacca taaaaccctt aaaataatac
     1741 ccttcttatc atcatactca tcttctgatt attttttcgt tgatgcacga tgcaaatgga
     1801 catccagttg tggaaaagca atctcgatcc cttcagctaa aaatcgtcga ttgatctcca
     1861 tatttaagtc attaacaaga tcaaggcgat ccgaaaattg acccgcatat actcgtaact
     1921 caaaattcaa ggtgcttgcg ccaaaatcaa caaaaacagc cagaggctca ggctctctca
     1981 ataccaatgg gtggttgtga gcaatctcta gcaatacctt tcttgcgtta tcacaatctg
     2041 atccataagc aatcccgact ggtattttaa tgcgcgttac ctcatcactg agtgtccaat
     2101 taataaattg ctgagtaata aaagtcttat tgggaacgat gacttcttta cgatctgagt
     2161 ctatcagcgt cgtggcacga atatgaattt tactaaccgt accagttaca tcgcttaatg
     2221 tcaccgtatc tccgattcgg atcggacgtt caaatagtaa aattaggcct gatacgaaat
     2281 tagccacaat ctcttgcaaa ccaaatccca ctccgacacc cagcgcggct actaaccaat
     2341 gaatattgga ccattccaca ccgagctttt gtgacatgat cgctattcca atgataatga
     2401 ttacgtattt taataaggtc gttatggcat agcctgtacc ggtatccagt gataaatggc
     2461 gcaaaaccaa aatttccaaa gtacccggta aattatgtgc cgcgataaat gcgagcacga
     2521 gaataatcat tgccaccagc agtgtttcaa gtgtgattaa ccggacgatg gtttctcctt
     2581 cacttatcgc agaagtccgc caaataacga tcttatctaa gaatcccagt gtcggtagca
     2641 aatcaaccca tatactgctc aaaccgagta acaaaagacc ccatacactg attgttagta
     2701 atgtatgaga ttgcttactg atcgtctcga catccatgta attctcatca accaattcca
     2761 cttccgcttt accggcaatc gaagtacgta acgcgatcat ttcttctctt ttttcaattg
     2821 ctcttttata ggtaatccta cgctgtgcta ttaataagcc acgataagct tgcagaaaaa
     2881 tcaaaccgca aatcgtaatc caagcaatcg tctcaaaaat aaggatggct tggtacaaag
     2941 cagcaaaata gtatcctatc gccgcagtag caattaaata caggagctgc aaaaagaata
     3001 tccaagccca tagctttgga ttttttaggt aattaaagtg ccggtctcga tacaatgctt
     3061 ttccgacttt ggttaccccc atccaatcta aagacaataa agcaagcaag aagcacactg
     3121 tgatgaaact cactcttccc acaacgtggc gcaccatgtc ttcaccaatt gcatccgtga
     3181 aagccgtaat caagcagaga ataacaataa ccggcccata atgtctgata ctcgaatgta
     3241 actgggctac aaaatctact ggaaatttaa aatgttggtt aagtagcccg gatggtcgac
     3301 taatctggta caacacattc cagaaaacaa ctgcaatcgc tgctacataa aaaacctggg
     3361 aaaatgcacg attgatctca gtattaacac tatgttcgag cgcgagataa atgaggaaga
     3421 atggtgacgc aaacaacaat gattgagaaa atatgaaaat cagcagtcgt accgagtaaa
     3481 ttatcttatc ttgcttaact tttccccatg cttcctttcc caccttctcc cacattaaat
     3541 agcgtggcca cagaaaccac cagaatacgc tgagcaaaat aaatgaaagg atcgtaacaa
     3601 tgaatgatac tagccctacg cgatcaacta gttttgaaaa agcgctctgg gttttatcgg
     3661 taagaaacca tgtcattgct ttatcaatat cattggtagt tacctggtaa attggttttg
     3721 cattacgggt tagtaataga ttttctttaa gtaggttttc atattgatca attttttcca
     3781 ataattgatg ctgcaagctc gcgtattgct ccaactcttt taaatattca cgcaaaattt
     3841 ctatgaattt atcaataact tgcaagcgga acattttaag ttcttgaaat gcccgcataa
     3901 tatcggggta aactgcagca tccgggttga ttgtatagcc cttagtcatt ctttttaaat
     3961 aagttttatc atcctgcatc tgaagctttt cttgctccag ttgaaacagc tccagctttg
     4021 cattgttaag tttcttttgt gtgagctgag tattaggttt tgttaaaaca tacttaaatt
     4081 gtttcctaat ttctgaacct agcgcttcgt catcctcata caatgcaagc ctctgctgca
     4141 acgcagcata attattgatg attagattca atcgattttc gctacgtgct ttctgtacga
     4201 ttaacttaga agattgttct acatagtgac tcagctctgt tgcaagcaat tcatttttct
     4261 taacgatgat gagaagaatg ggatgtttcc actcactttc ttctaggacc agccctgatt
     4321 tctcgacagc aacctctgtc tcggcttttc gctttaaatt aatttcaacc gctaaccatt
     4381 ctgattcttc aattaattgt tgtatctctg gcctaagtat gaattcttct tggaatgtcg
     4441 ccatttcgat ggctttgggt aacgcgagtg cctctagctc gagcattctc attttatccg
     4501 ccaatgcctg actttgaagc atttcataca tgcgtaatgc ttctacaatc ttctcattac
     4561 ttaaatcaag ctcagcaaat atttcagccg attcaatgtt acgcgtactg tttgcagtcg
     4621 ctagctcgtt acgattagtg acaacttgct ctcgcaatgt ggctagctca gataggattt
     4681 tttgctgttg attctgaaat tctgataatt tggcctgcgt ctcaatgttt ctctgcgcca
     4741 tttcacttaa tgtgagttgc ttcaattcaa agaaattagg ttgagtcgaa tttacattag
     4801 ttttttgttg ctctagattt tctagttgct gtgggaattg cgctaattgc tcagtatagt
     4861 attgagtttt ggcaaggtag ctttgatgat caagtaaaac ttgttgtgtt ttaagaaaaa
     4921 tctctttgag acttttcttt tggactgttt catcgctact attgagcgct tctaacttag
     4981 tttggatcga ttgaagttga tcatttaaat caatcgcttg acccaatacc gaggtagcaa
     5041 taaaacaaat aaaaaaaaca acaatactca ataaccgaat catttaggat atggcctgaa
     5101 tatcttaaaa tacagctcaa taaaggcaac cagatgttgg taaataatcc gtttcgatcg
     5161 cttatttttc attgctacta gcgcttggtt tattttcttg ttttgtggct aactcattcg
     5221 aggctgagaa acctttcttt attgacattc ctgcaaggaa taatcctaat tcatagagta
     5281 aataaagtgg aatcgctaat aagaactgcg agatgacatc tggtggggta aaaatcgctc
     5341 cgatcacaaa agcaccgaca agcacatact gccttacttc tttcattttg gttaacgaaa
     5401 taattccggt tcgagccagt acaactacaa ataccggtac ttcaaacgta atcccaaaag
     5461 tcaaaaacat cgtcatgaca aaaccaaggt actccccgat atctgtcatg acagcaaccc
     5521 cttgcggcgc aaaataaata ataaactcaa aaactaaggg taaagctgca aaataggcga
     5581 aggccatccc tataaaaaat aaaccgctac tcgccacgac caacggtaag aataatcgtt
     5641 tttcatgggc atatagcccg ggggcgacaa atgcccaaat ctgatacagc gtatgaggaa
     5701 gcgttaataa aaatgctgtc atcagcgcta ctttcattgg cacgaaaaaa ggtgtcgcca
     5761 ctgcggttgc aatcatttgc ccaccttgcg ggagtttttc gagaaggggc tgagctaaca
     5821 atgtgtatag ctcacgggca aaataagcac agggtataaa accaaccact aataccgtaa
     5881 taatccggat taaccgagtg cgtaattcga ccaaatgtga aataaaacta ccttcctcgt
     5941 ccatatttta tgactcactc tagaaaaaac tctatgaaag catactgtta tctctcaaca
     6001 cgacagtcct aattattatt agtcgaatca gtcgcccttt gagttcgaaa tcgattgaga
     6061 cgcaatgggc gtggcctcat gccctgcgtg attgttattt acgggagagg tatctttgat
     6121 ttttttatcg tggctatcgg atgcagtggg ctggacactt gcttgaattt gctgaatctc
     6181 ttgttttacg gatttttcaa tggattgcgc tgtatcctga accgtgtctt taatccgcct
     6241 taactcgtcc aattctattt cattccgtat atcatttttg accgtttcga catagcgacg
     6301 tgcgcgaccc aacaagaatc ctaaggttcg agctactcgt ggaagtctct ccgggccgat
     6361 gacaactaat gctacaactg aaatgacgag caactcagcg aagctaatat caaacataag
     6421 cctaaaatcc ttccgggaaa tttagaattc aaactactct agtttaagtt cttatagcag
     6481 gatattgaat ttccagagct taattcaact cccttattgg aatataacta aacgctttat
     6541 aatttagtct cgttcttttc ttttatacta acgtcttcgg tagatcgatt cgcaatttgc
     6601 tgctgagtat ttgcaggaga ttccattggt tctccttcgc ttcctttcat gccttcttta
     6661 aaacctttca ctgcattgcc tagatcactc cctaaattac gcaatttctt ggttccgaaa
     6721 actagcacta cgattgcaag tacaaccagc caatgccaaa tactaaatgt tcccattttt
     6781 atttcctctc aatagaatca ctattgacta cggtgaatca tagccggcaa aacattttta
     6841 ctgcctatca catgaatatg aagatgaaaa acttcttgcc cgcctacctt tcctgtattg
     6901 atgatagtac gaaaaccatg ttcacaccct tcctgtttgg ccaacttagg caccaggcct
     6961 aacatttttc caagaatgag ctgatgggta tcatcaactt catatagcga agcaatatga
     7021 agtttaggga taattaagaa atgaacggga gcagcagggt ttatatcatg aaaagcaagg
     7081 cagtgctgat cttcatagat aatctttgca ggaatttctt ttcttacaat tttacaaaaa
     7141 atacaatcgc tcatttactt ttccgtattg cgtagccgtt tttcttctat cccagataat
     7201 ccttctcttc gtgctaattc cgctaggaca tcttctggtc tcaggccatg gtgtgcaagt
     7261 aggataaggc aatgaaacca gagatcagca acttcataaa ccacatgtgt cgtatcgcca
     7321 tctttggatg ccatgatcgt ctcagccgcc tcttcagcaa ctttttttaa gattttatct
     7381 tgaccactat tcaatagctt agcaacatat gaggttgatg ggtcggctgt tcttctgtca
     7441 tcaatgacgg ttgcgagatg atgaagtatg gaggcgtgtg acatttattg atagatttct
     7501 gaaggatctt taattatggg cgcattaacg acccattgat ccccttccag tttattaaaa
     7561 aaacaacttt ttctaccggt atgacaagct atcccgccca tttgttctac ttgaatcagt
     7621 agaacatcat catcacaatc cagataaatt gctttgacct tttgtgtatg gccagattct
     7681 tcaccttttc tccagagcct ttgcttagaa cgagaccaat aaactaccac cttcgtttca
     7741 accgtcaatt taaccgcttc tttgttcatc caagcaaaca tcaaaacagt cccggttgct
     7801 tcttcttgtg caatgaccgg cactaaccca tcagtggtcc aattaatttt atcaacccaa
     7861 gaagctggca ttctgattcc agaaaattaa aaaatgatga caacctagtt attgttagag
     7921 tcgtacttcg ataccatgct gggataaaaa ttcttttgcc tcaagtatcg tatgctgac
//
LOCUS       DAOQKQ010000121         7792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_4555_length_7792_cov_5.627633, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000121 DAOQKQ010000000
VERSION     DAOQKQ010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 7792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7792
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4555_length_7792_cov_5.627633"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="PKZ49_08730"
     CDS             <1..789
                     /locus_tag="PKZ49_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HND36685.1"
                     /translation="SGDTGWQGIGRVSGRPWMEDDTKFMHVGISAGHTEVNTQYRADG
                     TMVGEGQIGGGGGMSFFAFPGTNVDRTNILNTGNLSTGALNDPNRRQVTSYDRYGAEG
                     WFVYGPFSAQAEYLRTNINGVGYDGEHLTGYYGFISYFLTGESKSYHVRNGAANRLKP
                     RKPFHWDGSGLGAWEIAAGYDYIDMNSGAIKGGEMDMFRFGLNWYPHSNVKFQSNIIH
                     VFNINTAGTPITNTNGYSGGAGARTHGWNNGDLSAFLTQITVDF"
     gene            838..1917
                     /locus_tag="PKZ49_08735"
     CDS             838..1917
                     /locus_tag="PKZ49_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HND36686.1"
                     /translation="MNHTLAARCSNAIALLEDKMLQLPHYLNRFSFVVLLFVCSHLAL
                     VAHAQPTINIDGSSTVYPITKIVADQFQITKNNTVTTVVDISGSGGGFKKFCRGEIDI
                     VNASRPILRDEIQQCKNSGIQYIEIPIAYDALTVAINPANNWSTRMTIAELKKMWEPA
                     AQGNITKWKQINSAWPDEPISLYGADEDSGTYDYFTEAIIGRAKSIRNDFTQSENDNH
                     LVDAVASNKNAIGFFGFAYYIENQNRVTAVAINNGRGAILPSTDTVEDGSYQPLSRPV
                     FIYVNIKSTDKAEIKEFVDFYLKNALLLVKEVKFFPLPPRAYKTMLEHFNSKRVGTIF
                     NGRPAIGLTIDELIRREGRLQYENY"
     gene            complement(1931..2827)
                     /locus_tag="PKZ49_08740"
     CDS             complement(1931..2827)
                     /locus_tag="PKZ49_08740"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HND36687.1"
                     /translation="MSDILNKILTVKVQEVAAAKALKSLSNIQIVAESSGQARDFVGA
                     IRTKLNQGQPAVIAEIKQASPSKGLLRGRIVSHQAATDLTLGRTQDVVIEGFEPAAIA
                     ASYAQQGAACLSVLTDRQFFMGSADYLKQARAACSLPVLRKDFMIDAYQIYEARAMGA
                     DCILLIVSAFLLFPYHDESGITDPLERMRYLEKLSHSLGMAVLVEVHDADELALALQL
                     STPLIGINNRNLRTFETRLDTTLQLLDRIPSERIVITESGIKEKADIHLMRSHQVNTF
                     LVGETFMRADDPGIAMQQLFFA"
     gene            complement(2842..3867)
                     /gene="trpD"
                     /locus_tag="PKZ49_08745"
     CDS             complement(2842..3867)
                     /gene="trpD"
                     /locus_tag="PKZ49_08745"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HND36688.1"
                     /translation="MSPQEILTRIIQHQDIPHDDMLVLMRQIMQGELSPVLIAAIITG
                     LRVKRESIDEIAAAAQVMREFATSVEVLDTDHLIDTCGTGGDTSHTFNVSTASVFVAA
                     AAGANVAKHGGRSVSSKSGSADVLEALGVGLNQTPQQIAQSIADIGVGFMFAPNFHSA
                     MKHAAPVRRELGVRTLFNILGPLTNPAGAKNQLLGVFDAKLVNLLVSVLHRLGSRHVM
                     VVHGFDGLDEITITGKTLIGELKQGERLEYTIQPEDFGMRSAPIEAIRVSDLNQAKDM
                     LLAVLENEPGPARDIVSLNAGAAIYISGIAPSLSQGIAVAQETLASGAAKAKLQALVN
                     YSRREAH"
     gene            complement(3898..4464)
                     /locus_tag="PKZ49_08750"
     CDS             complement(3898..4464)
                     /locus_tag="PKZ49_08750"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HND36689.1"
                     /translation="MLLMIDNYDSFTYNLVQYFGELGEAVEVFRNDEITLERIKALNA
                     QRIVISPGPCTPNEAGVSLSLLKEYGGQVPTLGVCLGHQSIGQAFGGKIVHAKQIMHG
                     KTSPIYHHNIGVFHGLPNPFTATRYHSLVIERESLPDCLEITAWTEDGEIMGVRHKSL
                     PIEGVQFHPESILSEQGHQLLKNFLTSR"
     gene            complement(4578..6434)
                     /locus_tag="PKZ49_08755"
     CDS             complement(4578..6434)
                     /locus_tag="PKZ49_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36690.1"
                     /translation="MIHTNSDNLGFGMSVRHTQKTVTSELTLGYRLKLGLFALLGHAV
                     VHGILLGSVIVLFAAVVWVDYAAPSDLTHVVLITLIPILLLLLSVLRIRLEKPQGYAL
                     RPTDFPELFTLLNDISQCIHAPRIDQVILTSELDITIIQTPKLGLWDWSHHTLVIGLE
                     LLLILSPQQANVALIHTLGHYSKKYHGFDNWIYQSRKNWQQLRNTLDRTHGVAPMLLK
                     HFFNWYLPKFNAYSAAVMQASEYQADALAAKMVHRDCVAKALISREVIGPYVDETYWL
                     AFFQQADDLPKPPSPWESLRHYLVNDLSMRVSLKRRLQQHLLTQCDTFEADPSLIDRL
                     AALNVHAELPEPSMISAAEIWFDSKYHRVIRDFDQMWLEINAKRWEERYEYVKQAKIR
                     LKYLRNFSLVKMTDEALWEKAQLEAELGDKDQAILAYRSYQDRFPDHALAAFHLGCYA
                     SAAGSDEVLIQMKKALTQADLVIPACQYACEYLARNHRKQESHWWQQQAERQLAIDHQ
                     ADVERTELADSDQIIAYESDDATSSYLAEKLRETGKITKAWIAQKIVQYYPEYPVLVI
                     VIECNGLFVNEMTLIEQLNKQLHLGCAFFVISKKGPHPSLAKQICALNDRII"
     gene            6912..>7792
                     /locus_tag="PKZ49_08760"
     CDS             6912..>7792
                     /locus_tag="PKZ49_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36691.1"
                     /translation="MSIPTETYEAVFEQYSAAMRWMTELGIKLNPGRTSHYEKIVGHW
                     KDAYKTATTDERRQTFPDFISSMFEVFDFVSIYKAFESVPVHQLTSIVEKLQKGVNGP
                     INAADERPDSTVARNFLFEVAVAAKAYRPDKGIETILDARSDTGISLSGKKLWVECKR
                     VTTIDKIESNVRKASSQLEAVLAREIGSGHRGIVALDVSKILNRGDKIFVTRNDDDLS
                     ASVDRIMDQFIEKYSHIWQLVYQRREKKIIGTIIRFSFMASSEARELLVYASQWAMNP
                     RLSVLESDEQIQRQLVAT"
BASE COUNT         2212 a         1806 c         1654 g         2120 t
ORIGIN      
        1 tctggcgaca ccggttggca aggaatcggg cgtgtttccg gaagaccttg gatggaagac
       61 gacacgaaat tcatgcatgt cggtatttca gcaggtcata ccgaagtcaa tacccaatac
      121 cgtgcggatg gcaccatggt cggtgaaggt caaattggcg gcggcggtgg catgtcattc
      181 ttcgccttcc ccggcaccaa cgtcgatcgt accaatattc tcaacaccgg taacttaagc
      241 acgggggcat taaatgatcc taaccgccgt caagtcacga gctatgatcg ttacggtgct
      301 gaaggctggt ttgtatatgg acctttctca gctcaagctg agtatttacg aaccaacatc
      361 aatggagtgg gatacgatgg cgaacacctt acgggttact atggatttat cagctatttc
      421 ctgactggcg aatcaaaatc ctaccatgtt cgaaacggtg cagcgaatcg acttaaaccg
      481 cgtaaaccct tccattggga tggttcaggt ctgggtgctt gggagattgc tgccggctat
      541 gactatatcg atatgaattc cggcgcgatc aaaggtggtg aaatggatat gttccgattt
      601 gggttaaatt ggtatccgca ctccaatgtt aaattccaaa gcaacatcat tcatgtgttc
      661 aatatcaata ccgctggcac accgattact aataccaatg gttattcggg tggagccggt
      721 gcgcgcactc acggttggaa taatggcgac ctcagcgcat ttctcacaca aattactgta
      781 gatttctaaa tcatgtttca aatgaataca aactggaaac atgttttaat tatgagaatg
      841 aaccatacac ttgccgctcg ttgcagcaat gcaatcgcgc tattggagga taaaatgttg
      901 cagttaccgc attatcttaa ccgctttagt tttgttgttt tattgtttgt ttgcagtcat
      961 ctagcgctag tcgctcatgc tcaaccgacc attaacatcg atgggtcgag tacggtctat
     1021 cccatcacta aaatcgttgc tgatcaattt cagatcacaa aaaataatac ggtaacgaca
     1081 gttgttgata tctcaggcag cgggggtggt ttcaaaaaat tctgccgtgg tgagatcgac
     1141 atcgttaatg cttctcggcc gatactccgc gatgaaattc agcaatgtaa aaattcaggt
     1201 attcaatata tcgaaatccc gattgcttac gatgccttga ccgtcgcgat caatcccgct
     1261 aataactgga gcactcggat gacaatagct gagctcaaaa aaatgtggga acctgcggca
     1321 caaggtaaca tcaccaagtg gaaacaaatt aactcagctt ggcctgacga accaatcagt
     1381 ctttatgggg ctgatgaaga ttcaggaaca tacgattact ttaccgaggc gattatcgga
     1441 cgcgccaaat caatccgtaa tgatttcact caatcggaaa acgataatca cctagtcgat
     1501 gccgtcgcga gcaataaaaa tgcaattggt ttctttgggt ttgcctacta tattgaaaac
     1561 cagaatcgag tcactgctgt tgcaatcaat aacggtagag gcgcgattct cccttccacc
     1621 gataccgtcg aagatggaag ctatcaacct ttgtcacggc ctgtttttat ttatgtgaat
     1681 attaaatcaa ccgacaaagc ggaaattaaa gaattcgtcg atttctattt aaagaatgca
     1741 ctgttgctcg tcaaagaagt taagttcttt ccgctaccac cgagggctta taaaaccatg
     1801 ttagaacact tcaatagcaa acgcgtgggt accatcttca acggaagacc ggcaattggt
     1861 ctcaccatcg atgaattgat tcgtcgtgaa ggaagattgc aatacgagaa ctactaattt
     1921 aacgcatccc tcaagcaaaa aagagttgct gcattgcaat accaggatca tcagcacgca
     1981 taaacgtttc tcccactaaa aaggtattca cttgatgcga tcgcatcaag tgaatatccg
     2041 ctttctcttt tatcccactc tctgtaatga cgatgcgctc cgaaggaata cgatctagta
     2101 attgcaacgt agtatcgaga cgcgtttcaa aagtacgcaa attacggttg ttgatgccga
     2161 tcaagggggt cgataactgt aaagctagcg ctaattcatc tgcatcgtgt acttctacca
     2221 atacggccat ccccaacgaa tgggaaagtt tctccaaata acgcatgcgt tctaatggat
     2281 cagtaatccc ggactcatcg tgatagggaa ataataaaaa agccgatacg atcaacaaaa
     2341 tgcaatcagc acccatcgct cttgcttcat agatctgata ggcatcgatc ataaaatctt
     2401 tacgcaagac tggcaggcta cacgctgcac gagcctgttt gagataatca gcactgccca
     2461 taaagaattg ccgatcggtc aggactgaaa ggcaagctgc gccctgttgc gcgtaactcg
     2521 ctgcaatggc tgcgggttca aatccttcga tgaccacatc ctgagtgcga cccagcgtta
     2581 aatcagtagc ggcttgatga gaaacgatcc gtcctcgcaa tagcccttta ctcggacttg
     2641 cttgtttgat ttctgcaatg acagccggtt gaccctggtt tagtttggtt cgtatcgcgc
     2701 cgacaaaatc ccgagcctgt ccagaagatt ctgctactat ttgaatgttg gataacgatt
     2761 ttagcgcttt tgcagccgca acttcttgca ctttaacagt aagaatttta ttaagaatat
     2821 cagacataga taacggggta tttagtgtgc ttcccggcgg gaatagttaa ccaatgcttg
     2881 taattttgct ttggccgcac cgcttgccaa tgtttcttga gcaaccgcaa tgccttgcga
     2941 tagtgaagga gcgattccag aaatataaat cgctgcaccc gcattcagtg aaacaatatc
     3001 tcttgctggg ccaggctcat tttcgagaac agcaagcaac atatccttgg cttggtttaa
     3061 atcagaaact cggatagcct caatcggcgc actccgcatt ccaaaatctt ccggttgaat
     3121 cgtgtactct aggcgttcac cttgttttag ttcacctatc aaggttttac cggtaatcgt
     3181 gatctcatct aatccgtcaa acccatgcac gaccatcaca tgtcgacttc ccagtcgatg
     3241 gagcacgctg actaatagat tcactaattt tgcatcaaaa acacctaata actggttttt
     3301 agcaccggca ggattggtca gtgggcccaa tatattgaac aaagttctca cccctaattc
     3361 gcgccgtacc ggtgcagcat gtttcatcgc actatgaaaa ttaggcgcaa acataaaccc
     3421 aacgccaata tccgcaatcg attgcgcgat ttgttggggt gtttgattca aacctacgcc
     3481 aagtgcttcg agcacatccg cactccccga tttacttgat actgaacgcc cgccatgctt
     3541 cgcgacattt gcacccgccg ctgctgcgac gaacaccgat gcagtcgata cattgaaagt
     3601 atgtgacgta tcaccgccag taccacaggt atcgatcaag tgatcagtat ccaacacctc
     3661 cacactcgtc gcaaattcac gcattacttg cgccgctgcc gcaatttcat cgatcgattc
     3721 tcgtttgacc cgtaatccag taatgatcgc agcgattaga acgggtgata gttccccttg
     3781 cataatttga cgcatcaaga ccagcatatc atcatgcgga atatcctgat gttggataat
     3841 tcgagttaat atttcctggg gactcatatc aaaaaaattt ggaaaattta gctaagctta
     3901 gcgactggtc agaaagtttt ttaggagttg atgcccttgt tcactgagta tcgattcggg
     3961 atgaaattgt accccttcaa tgggcagtga tttatggcga acccccatta tttccccatc
     4021 ttcggtccag gccgttattt ccaggcaatc tgggagactc tctcgctcaa tcaccagaga
     4081 atgataacgg gtcgcagtaa atggattggg gaggccgtga aatacaccta tattgtgatg
     4141 gtatatcggt gacgtttttc catgcatgat ctgcttagcg tgcacaattt ttccaccaaa
     4201 tgcttgccca atgctctggt gtcctagaca tacccctaac gtaggaacct gaccaccgta
     4261 ttctttcagc agcgataagg acacgcccgc ttcattaggc gtacaaggcc caggtgaaat
     4321 aacgattcgt tgtgcgttaa gcgctttaat tcgttctagc gtaatttcat catttctaaa
     4381 tacctcgaca gcttcaccaa gctcaccaaa atattgcacg aggttatagg taaacgaatc
     4441 ataattatca atcatcaaca gcatgctgca gctacttcct ttcaaaatct cgattagaaa
     4501 gactagttta tcacagcctt ctatcaactt gagttgaatg cagtaatcaa ctcagtcaca
     4561 ttggtctttc ttgaatctca gatgattcga tcatttagcg cacaaatttg cttagccaag
     4621 gatgggtgcg gtcccttttt tgaaatgaca aaaaatgcac aacccaaatg aagctgttta
     4681 ttcaattgtt caatcaatgt catctcattc acaaataagc cattacactc aatgacaatg
     4741 accaacacag gatattcagg ataatactga actatttttt gagcgatcca cgctttggtt
     4801 atttttccgg tttcgcgtag cttttcagca agataagatg aggtagcatc atcactttcg
     4861 taagcaatga tttgatctga atcggctaat tctgttctct caacatccgc ctgatgatcg
     4921 atagctaatt gacgttcagc ctgttgttgc caccaatgtg actcctgctt gcggtgattg
     4981 cgagctaaat actcacaggc atattgacaa gctggaataa ctaaatctgc ctgtgtcagt
     5041 gcttttttca tttgaattag cacttcatca cttccggccg cacttgcata acatcccaga
     5101 tgaaaggcag ccagcgcatg atccggaaaa cgatcttggt aactccggta tgccaagatc
     5161 gcttgatcct tatcacccaa ttcggcttca agttgggctt tttcccatag tgcttcatcc
     5221 gtcattttta ctaaactaaa attgcgcaag tacttaagcc gtattttggc ttgcttcaca
     5281 tattcatatc tttcttccca acgtttagcg tttatttcca gccacatctg atcaaaatca
     5341 cggatcacac gatgatattt tgaatcaaac cagatttctg ctgctgagat catcgaaggc
     5401 tctggtaatt ctgcgtgaac gttcaatgcg gctaaccgat cgatcagcga aggatcggct
     5461 tcaaatgtat cgcattgtgt taataaatgt tgttgtaacc gccttttcaa gcttactctc
     5521 attgacaaat cattcactaa ataatgtcgc aagctttccc acggagaagg tggtttgggt
     5581 aaatcatcgg cctgctgaaa gaatgccaac caataggttt catcaacgta gggaccaatc
     5641 acttcacggc tgatcagtgc cttcgccaca caatcacgat gaaccatctt agcagccaat
     5701 gcatctgctt gatattcact cgcttgcata accgctgctg aataagcatt aaacttgggt
     5761 aaataccaat taaagaaatg tttcaacagc atcggcgcta ccccatgagt gcgatctaat
     5821 gtgttgcgta attgctgcca gttctttcta ctttgataaa tccaattatc aaagccatga
     5881 tactttttag aataatgtcc caatgtatga atcaacgcta cattggcttg ttgaggagaa
     5941 aggatcaata gcaattcaag accaataacc agggtatgat gagaccaatc ccataagcct
     6001 aatttgggtg tttggatgat cgtaatgtct aactcgctcg tcaaaataac ctgatcgatt
     6061 ctgggcgcat gaatacattg actaatatca ttcagcaaag taaaaagttc cgggaaatca
     6121 gttggccgca acgcatagcc ctgaggtttt tccaatcgaa ttcttaaaac tgaaagcagt
     6181 aataaaagaa tcggaatcaa ggtaatcagc acgacatgcg ttaagtccga tggcgcagca
     6241 taatcaaccc aaacgacagc cgcgaacagc actatcactg acccaagcaa tatcccatgc
     6301 acgaccgcat gccccagtaa cgcaaacagc cctaatttaa gccgatagcc cagtgttaac
     6361 tcacttgtga ccgttttctg agtatggcga acagacatac cgaaccccaa gttatcacta
     6421 ttggtatgaa tcatgaaaat tacctataaa gtttgataag aagcctaccc aagccatgaa
     6481 tgcatcttga aaaatgacgc agaccaggca agcgattgtt aaaagaataa tttgggtcag
     6541 ccatttgatc tgtgcgttaa cgcacagtaa tagatctagc gttggagttc cccattttat
     6601 agggcagcct tatcaaacct ggccgtgaaa ggtagcgcaa ctagcaaaca cttcattcaa
     6661 gcaatatcaa attgctcagt acgaaactgt aatacttaaa cctgagtttt ccccgtggct
     6721 aatttagaag ttggcgtttg ttttctgttt aatgattcat gtaatttgta agcacaaaat
     6781 ttggtatgat gataactaat tcgttcaatc gttatagtca tgaggaaatt ccatgcctaa
     6841 ttccgtatta gatctcattt gtgacgtaat aaatcgcgtc atttcggaca tctaattcta
     6901 gttaggttcg catgtccatc cccaccgaga cttacgaagc cgtctttgag cagtattcgg
     6961 ccgcgatgag gtggatgact gaacttggta tcaaactgaa tccaggaaga acctctcact
     7021 acgaaaagat cgttggacat tggaaggatg cttacaagac tgcaactacg gatgaaaggc
     7081 gacagacatt cccagatttc atcagttcaa tgttcgaagt atttgacttt gtgagcatct
     7141 acaaggcgtt tgagagcgtc ccagtccatc agctcacatc tatcgttgag aagcttcaaa
     7201 aaggtgtgaa tgggccgatc aacgccgcag acgaaaggcc agactccacc gttgctcgca
     7261 atttcctttt cgaggttgca gtcgcagcaa aggcttatcg ccctgataaa ggtattgaaa
     7321 ccattctcga tgcgcggtcc gatactggca tcagtctgag tggaaagaag ttatgggtcg
     7381 agtgcaaacg cgtaacaacc atcgacaaga tcgagagtaa tgttcgaaag gcttccagtc
     7441 agctagaagc ggtacttgcg agggagatag gttctggcca ccgtggaatt gttgccttgg
     7501 atgtttccaa gattttgaat cgcggtgaca agatttttgt gacgaggaac gacgatgatc
     7561 tgtcggcgtc cgtggatcga ataatggatc agttcattga aaagtactct catatctggc
     7621 aactcgttta ccagagaagg gagaagaaaa tcatcggaac gatcattaga ttctcgttca
     7681 tggccagttc ggaagccaga gagctcttgg tgtatgcgtc gcaatgggct atgaacccgc
     7741 gcctgagcgt ccttgagagt gacgagcaaa ttcaacggca gctcgttgcc ac
//
LOCUS       DAOQKQ010000122         7761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_4580_length_7761_cov_5.775110, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000122 DAOQKQ010000000
VERSION     DAOQKQ010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 7761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7761
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4580_length_7761_cov_5.775110"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..2354)
                     /locus_tag="PKZ49_08765"
     CDS             complement(96..2354)
                     /locus_tag="PKZ49_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HND36692.1"
                     /translation="MNIPLFQFIVFILLLSLSVFSVDHAVAQQINLKKEMKELPIHVE
                     ADRIEGFAERHMEAIGKAQILQGDQFIAADRIKYYRGTQEVEVKGKVRFERDRDILEG
                     SNLKLFLDTKTGELSLPQYILKDGGGRGAGQLFLFEGDDQYRLKKGNYTTCPIGNDDW
                     YIRAEDLEIDNKKQVGTARDVTVRFKDVPILYLPWMNFSYSGQRKTGLLAPVFGNTIR
                     SGVEVSIPFYWNIAPNIDATFTSRNMSRRGAMLNTEFRYMGQSHGGQLNFDILPNDLE
                     TGKTRYGLAFEHMQHLGGGWTGNVNYNQISDREYFRDLATSVAFTSRANLVQQAGLSY
                     VGSLGHDGVINFNAMVQGFQTIQDPRAPIISPYKRLPQMTLNMSKRNVFGTDLDFTGV
                     YTHFYRPDSQQVPESQSGHRMVMFPSISVPLQTSYGYIKPKMGVHFTRYELGDSLKDV
                     NVTDTNPQRVLPIFSLDSGVVLERDTKLGRTNFVQTLEPRLYYLYIPFRNQNRLPNFD
                     SAEMDFSFAQIFTENRFSGHDRINDANEVTFAMTSRLIEQSTGNERVRIAAGQRVRFT
                     ERDVVLSNSQITSVKSDFIAAISGRITPVITTDSSIQLDQSELRTEKIRTGISYHPAP
                     GKVLNVGYRFTRGIPGIERGNNLFPVPGAGFEQVDFSTQWPVLKNWQAMASANYSLDD
                     DKLLAGLVGLEYNACCWSLRFVMNRFVRATGKESTTFFVQLELNGLMRIGTNPLAALR
                     QGVPGYTRTSHQ"
     gene            2483..4438
                     /locus_tag="PKZ49_08770"
     CDS             2483..4438
                     /locus_tag="PKZ49_08770"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HND36693.1"
                     /translation="MSDLAAIFSAQGVLAHTIPNYRARIEQLEMAQAIAKTIQHQQIL
                     VAEAGTGTGKTFAYLVPALLFGGKVIISTGTKTLQDQLYDRDIPTIRAALKVPVTVAL
                     LKGRANYVCHYRLEQTLESDQYKLTTREDIIHLGLIERFAQNHQTGDKSSLSKVPENA
                     SIWQHVTSTRDNCLGSDCPHFKQCFVMEARKKALLSEVIVVNHHLFFADLMLRDEGLS
                     ELLPTCNTVIFDEAHQLPEIASLFFGESVSTSQLLELARDSEMETRLAAPDYKPLLDC
                     IEAFEKTTRDLRLTIEQDNARLSLNHALRSNHFSAALALLQQRLTELVNLLEHQAVRS
                     PGLEQCWKRTTIIHSSLNRWQDQLEQEGYVRWVETFNQALHLNATPLSIAETFSKQLS
                     VPPRAWIFTSATLAVKDDFSHYQQAMGLAQAQSIRWESPYNFAHQALLYVPTGLPSPN
                     HQHFTAALIEAVLPVLKASQGRAFILCTSLRAMHEIHALLQTQFEQHQLTYPILLQGQ
                     GSRSTMLERFRELDNAILIGSQSFWEGIDVKGEALSVVIIDKLPFASPDDPVLSARID
                     QLTKEGHNAFMEYQLPRAIINLKQGAGRLIRDEHDRGVLMICDPRLITHAYGKRIWQS
                     LPPMKRTRNFLDVQKFFSPQPIQPINS"
     gene            4551..5831
                     /locus_tag="PKZ49_08775"
     CDS             4551..5831
                     /locus_tag="PKZ49_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HND36694.1"
                     /translation="MNKNIKMMLFGFVSLLLLCCGSLVIAAEPASSVNEARLVAQYNY
                     TIHILAMLLVGFGFLMVFVRRYGFGATTGTYLVVATGLPLYMLLRANGIFGHELNPHS
                     VDAILYSEFAVATALIAMGAVLGRLRVFQYALLALLIVPAYLLNEWLVLDNASGLTEG
                     FQDSAGSIVIHAFGAYFGLAMSLVLTTAQQREEPIVSDATSDRFAMLGSMVLWLFWPS
                     FATAIVPFEDMPQTVVNTLLALSGATLATYFLSTHFHKGKTSMVDMANAALAGGVAIG
                     STCNVVGPTGAFMIGLLAGALSVIGYVFIQPWLESKLKLIDTCGVHNLHGMPGLLGGL
                     SAIVVVPGIAVVQLTGIGITLAIAITGGLIAGAIIKATGTTEQAYEDSHEFTHLEGPE
                     SETRVEQLALDAKKGVDDLKREVSSRATASQPRF"
     gene            complement(5940..>7761)
                     /locus_tag="PKZ49_08780"
     CDS             complement(5940..>7761)
                     /locus_tag="PKZ49_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HND36695.1"
                     /translation="FFQNQFNFTQDLRIAIRGFGARSPFGVRGIQMRVDDIPQTLPDG
                     QTQLDAIDPAMIERMEVLRGPSASLYGNASGGMIHITTRNPQHAFEVSPRLVFGQYGL
                     IKSELYAGGRTENFNYGLFGSYLQQAGWRDHSRMENLFSQVKINFKTGSDSDWMVLLH
                     KFYSPDSKDPGALTLAESRANPKQASANNQLFNASESINQEQFAVRYRKNVAPGQLIT
                     ITTHALHRDFQNLLPFTNGGQVEFNRWVGGAMLQYSSDHTLWQRPNRLLAGIDYGIQN
                     DYRQRYNNHFGERGLLNLNQIERVQNIGLFLRNEWRIFDRLDFVVGGRWDWLHYQVKD
                     TFLADDNQSGSRTLSQGSGTAGLVYHLGDAHQLYTHVATIFEAPTTTELINNPTGKGG
                     FNPNLNAQTSLSKEMGIRGQPSGFEYEATLFHVGTRNEITPFELPDSPGRAFFRNAGQ
                     SERFGLETRLSSPSWNGFRADMTYTYSDFEFKRYRVGNDDLSGNEFPGLPRHRWEGRV
                     RYTHPVGFFTQFHVHRIGEFFVNDINTVTNSAYYLGQFLLGWEKKYHWIEGSIFLGIN
                     NLFDERYNANTRINAALGRYFEPGPPLNVFGGARIRLWVF"
BASE COUNT         2209 a         1843 c         1589 g         2120 t
ORIGIN      
        1 aaaacaatac tgatagttaa gctgataagt acaaaaaata gttttttcat taaattatcc
       61 aacaacatat tgattgactc ctataccaat ctgatttatt gatgacttgt ccgggtatag
      121 cccggcactc cttgccgcaa cgcagcaagc ggattagtcc cgattcgcat caatccattc
      181 aattcaagct gcacgaagaa agttgtcgat tcttttccag tagctctgac gaaacgattc
      241 attacaaaac gcagcgacca acaacatgcg ttgtattcta atcctactag tccagcgagt
      301 aacttgtcat catcgagtga gtaattagcg ctagccattg cctgccaatt ctttagaact
      361 ggccattgcg tagaaaaatc aacttgttca aaaccagcac cgggcactgg aaagagatta
      421 ttccctcgct cgatccccgg gataccccgt gtgaaacgat aaccaacatt caacacctta
      481 ccgggtgcag ggtgatagct gatacccgtt cgaatttttt cagtacgcaa ttcactttga
      541 tctagctgaa tactactatc tgtggtaata accggggtaa tacgccccga aatcgccgca
      601 atgaaatctg atttaacact ggtaatttgc gaattggaca atacgacatc tcgctcagta
      661 aagcgaactc gttgtccagc agcaatacgc acacgctcat tccccgttga ctgttcaatt
      721 aagcgcgaag tcatggcaaa ggtaacctca ttggcatcat tgatacggtc atgcccacta
      781 aaacggtttt ctgtaaagat ttgcgcaaag ctaaagtcca tctcggctga atcaaaatta
      841 ggcaatcgat tctggttacg aaacgggata taaagataat aaagacgcgg ttcaagcgtt
      901 tgaacaaaat tggttcgacc cagcttagta tcgcgttcaa gcacgacacc gctatccaaa
      961 ctaaaaatag ggagtacccg ttgcggattg gtatcggtca cattgacatc ctttaaggaa
     1021 tcacctaatt cataccgagt aaaatgaaca cccatcttag gtttaatata accatacgat
     1081 gtttgtaagg gaacgctaat actcggaaac atgaccatac ggtgaccgct ctgcgattct
     1141 ggaacttgct gcgagtcagg tcgataaaaa tgcgtatata ccccggtaaa atcgagatca
     1201 gtcccaaata cattgcgttt cgacatattc aatgtcattt gcggtaaacg cttatagggt
     1261 gaaataatcg gtgcgcgcgg atcttgaatc gtttggaaac cttgaaccat ggcattgaaa
     1321 ttgattacgc catcgtgccc taatgaacca acataggata atccagcttg ttggaccaag
     1381 tttgctcgcg atgtaaatgc aacggaagtt gctaaatctc gaaaatactc acgatccgaa
     1441 atttgattat agttaacatt gccagtccaa ccgcctccta aatgctgcat atgctcaaaa
     1501 gcaaggccat aacgggtttt tccagtctcc aaatcattcg gtaaaatatc aaaattgagt
     1561 tgaccgccat gactctggcc catatagcga aattcagtat ttaacattgc gccccgtcga
     1621 ctcatattac gcgatgtaaa cgttgcgtct atgtttggtg caatgttcca gtaaaacgga
     1681 atcgatactt cgacaccact gcgaatcgta ttaccaaaga ctggcgccag tagtcctgtc
     1741 ttacgctgcc cactatagga aaaattcatc cagggcaaat aaagaatggg aacatctttg
     1801 aaccgaactg tcacatcccg agcggttcca acttgctttt tattatcaat ttctaaatct
     1861 tcagcccgaa tataccaatc atcgttacca atcggacagg ttgtgtaatt accttttttc
     1921 agtcggtatt gatcgtcccc ttcaaataaa aataattgcc cggcaccgcg tccaccgcca
     1981 tctttaagaa tatattgagg aagacttaat tcaccggttt tagtatccag aaataactta
     2041 aggttacttc cttcgagaat atcccgatcc cgttcaaaac ggactttacc tttaacttcg
     2101 acttcctgag ttccccgata atactttatc cgatctgcag caatgaattg atcgccttgt
     2161 aaaatctgcg ccttgccaat ggcttccata tgccgttcag caaaaccctc gatacgatcc
     2221 gcttcaacat ggatgggtaa ttctttcatc tcctttttta aattgatttg ctgcgcaacc
     2281 gcatgatcta cgctaaacac tgatagactt agcagcaata taaaaacgat gaattgaaac
     2341 aaaggaatat tcattaatgc gataccgaag ccatttcaaa gcaaatttgc aatggcagca
     2401 agcttaaggt agataacact cattattgga caactttatt cgcgattact caaaaccgct
     2461 attttaactg acaatcattt tcatgtctga tcttgcagct attttttctg cacaaggtgt
     2521 tcttgcccac acgattccta attatcgagc cagaattgaa caattagaaa tggcgcaagc
     2581 catagccaaa acgattcagc accaacaaat cttggtcgct gaagcaggca ctggtactgg
     2641 aaaaaccttc gcttatcttg tgcctgcttt gttattcggt ggaaaagtta ttatctcgac
     2701 aggaaccaag acgttacaag atcaactcta tgaccgcgat atcccgacga tacgcgcagc
     2761 acttaaagtt ccggttaccg ttgctttact caaaggccgc gccaactatg tctgtcatta
     2821 tcgccttgaa cagaccctcg aatccgatca atataagcta accacacgcg aagatatcat
     2881 ccaccttggc ctcatcgaac gtttcgccca gaatcaccaa accggcgata aaagcagctt
     2941 aagtaaagtt cctgaaaatg cgagcatttg gcaacatgtc acatcgaccc gtgataattg
     3001 tttaggttct gattgtcctc atttcaaaca atgcttcgta atggaagcac gcaaaaaagc
     3061 attattgtct gaagtcattg tcgtcaatca tcatttattc tttgcagact tgatgttgcg
     3121 tgacgaagga ctctctgaat tgcttccaac ctgcaatacc gttattttcg atgaagcgca
     3181 tcaattaccg gaaattgcta gtctcttttt tggagaatca gtgagcacga gtcagttatt
     3241 agagttggca cgtgacagtg aaatggaaac cagactcgct gctccagact ataaaccgtt
     3301 actcgattgt atcgaagcat tcgaaaaaac aacgcgcgat ttacgcttaa ccatcgagca
     3361 agataatgcc cgtttatccc tcaatcacgc gctacgctcc aatcatttca gcgctgcact
     3421 tgcgctactt caacaacgac tgactgaatt agtcaatcta ctggaacatc aagccgttcg
     3481 gtctccaggg ttagagcaat gctggaaacg aactacaatc attcacagta gcctcaatcg
     3541 ttggcaagat cagctcgaac aagaaggcta tgtacgctgg gttgaaacct tcaaccaagc
     3601 actccatctc aatgccacgc cgctttctat cgcagaaaca tttagcaagc agctcagtgt
     3661 tccaccacgc gcttggatat tcacttcggc cacgcttgcc gtcaaggatg atttttctca
     3721 ttaccaacaa gcgatggggt tagcacaagc gcaatcgatt cgttgggaaa gtccttacaa
     3781 ttttgcgcat caagcattac tctacgtgcc aaccggcttg cccagcccta accatcaaca
     3841 cttcacggct gcacttatcg aagcggtcct gcccgtgcta aaagcaagcc aaggtcgtgc
     3901 tttcatatta tgtactagct tacgtgccat gcatgagatc catgccttat tacaaacgca
     3961 attcgaacaa caccagttaa cttatccaat tctgcttcag ggacaaggat cgcgatcaac
     4021 catgctagag cgcttccgtg aattagacaa tgcaatactc atcggcagtc agtctttttg
     4081 ggaaggcatt gatgtgaaag gtgaggcact atcagtcgta atcatcgata aattaccatt
     4141 tgcctcccct gacgatccgg tattatcggc acgcatcgat caacttacca aagaaggaca
     4201 caacgccttc atggagtatc aattaccccg tgctatcatc aatcttaaac aaggtgctgg
     4261 gcgactgatt cgtgatgaac acgatcgtgg tgtactaatg atttgcgatc ctcgtctgat
     4321 cacccacgct tacggtaaac gcatctggca aagcctacct ccgatgaaac gtacacggaa
     4381 ttttctcgat gtccagaaat tctttagtcc gcagccaatt caacccatca attcttaatt
     4441 caattatcaa aaattctctt ttattcccct attgaattta gggccattaa ggttcacact
     4501 gcgtagcggg gcaacccaaa ccaactcctc aattcataag ggaaattata atgaacaaaa
     4561 atattaaaat gatgttattt ggcttcgtca gtttgcttct cctgtgttgt ggatcgctcg
     4621 ttattgctgc tgagccggct tcgagcgtca atgaagcgcg gcttgtcgcg caatataatt
     4681 atacgattca cattcttgcc atgttattgg tcgggtttgg ttttttgatg gtattcgtgc
     4741 gccgctatgg gtttggtgcc acaaccggta cctatttagt agttgctacc ggactaccgc
     4801 tttatatgct actccgtgcc aatggtatct ttggccatga attaaatccg cattcagtcg
     4861 atgctatctt atattctgaa tttgcggtag caaccgcact catcgccatg ggcgctgtgc
     4921 taggtcgctt gcgggtattt caatatgcac tgttagcatt attgatcgta cctgcttacc
     4981 tcctcaatga atggctggta ttggataatg catcaggttt aaccgaagga ttccaggatt
     5041 ctgcaggatc gatcgtaatc catgcgtttg gcgcatattt cgggcttgcg atgtctttag
     5101 tgttaaccac tgcgcagcaa cgcgaagaac ccatcgtttc tgatgctacc tctgatcggt
     5161 ttgctatgtt aggttccatg gtgttatggt tgttctggcc tagttttgca acagcgatcg
     5221 taccatttga agatatgcct caaacagtag tcaacacttt gctcgcgctc agtggtgcaa
     5281 cccttgcgac ttatttcttg agtactcatt tccataaagg taaaacttct atggtcgata
     5341 tggctaacgc agcattagcc ggtggggttg cgatcggctc aacgtgtaat gttgtggggc
     5401 caaccggggc attcatgatc ggtttgctcg ctggtgcact atcggtcatt ggctatgttt
     5461 tcattcaacc gtggctcgaa tccaaactta aattaattga tacttgcggt gtccataatt
     5521 tacatgggat gcctggttta ctcggtggtc taagtgcaat tgtcgttgtt cctggtattg
     5581 cggttgtgca attgacaggc atcggcatca cactcgccat tgctattacc ggcggtctta
     5641 ttgctggagc aatcattaaa gcaacgggca cgactgaaca agcctatgaa gatagccatg
     5701 aatttactca tctagaagga ccggaaagcg aaacgcgtgt cgaacaactg gcactcgatg
     5761 ctaagaaagg ggtagacgat cttaaacgcg aggttagtag tcgagcaacc gcttctcaac
     5821 cgcgattcta atagaaaatc actataacac tctcgtgatc aattgatact gtcgtcgcca
     5881 agctagattg tgcgcacacg gttagcttgg ccgagcgtgt tgacaattaa cccgagatat
     5941 caaaataccc ataatcgaat cctggctcct ccaaaaacat tgagcggagg gccaggctca
     6001 aaataccgac ctaaagctgc attgatccga gtatttgcat tataacgttc atcaaataaa
     6061 ttattgattc ccaaaaagat cgagccttca atccagtgat atttcttttc ccagcctaat
     6121 aagaattgcc caagataata agcactatta gtaaccgtat tgatatcatt cacaaaaaat
     6181 tccccaatgc ggtgcacgtg aaattgtgtg aagaatccaa ctggatgggt ataacgtacc
     6241 ctgccttccc aacgatgtct gggtaaacca ggaaactcat tgccgcttaa atcatcgttg
     6301 ccaactcgat aacgtttaaa ttcgaaatcg gagtaagtgt aggtcatatc ggccctaaaa
     6361 ccattccatg agggactcga taaacgggtc tccaagccaa agcgctcgga ctggcccgcg
     6421 ttacgaaaaa aagcacgtcc tggtgaatcg ggtagttcaa aaggagtaat ttcgttgcga
     6481 gttccaacat gaaataacgt tgcttcatat tcaaatcctg aaggctggcc gcgtataccc
     6541 atttctttac tcaacgaagt ttgggcattt agattaggat tgaagccccc cttacctgtc
     6601 ggattattga tgagctcagt agtcgttggc gcttcaaata tcgtggcaac atgggtataa
     6661 agctgatgtg catcccctaa gtgatatacc aaaccggctg tgccacttcc ttgcgataag
     6721 gttcttgagc ctgattgatt atcgtcagcg agaaacgtgt ctttgacttg atagtgcaac
     6781 caatcccaac gcccccctac gacaaaatct aggcgatcga atattcgcca ttcatttctc
     6841 agaaagaggc cgatgttttg cacgcgctcg atttgattga gatttaataa cccccgttca
     6901 ccaaaatgat tgttatagcg ttgtcgatag tcattctgaa taccatagtc aattccggcc
     6961 aataagcgat taggccgctg ccataaagta tgatcgcttg aatattgcag catggcaccg
     7021 cctacccagc gattaaactc gacctgtcca ccattagtaa agggaagtaa gttttgaaaa
     7081 tcgcgatgaa gtgcatgggt agtaatcgta attaactggc ctggtgcaac atttttgcga
     7141 taacgaacgg caaattgctc ttgattaatc gactcactgg cattgaataa ttgattatta
     7201 gccgatgctt gcttaggatt ggcgcgtgat tcagccaagg ttaaagcgcc cggatctttg
     7261 gaatctggtg aatagaattt atgaagtaat accatccaat ccgaatcgga gccagtctta
     7321 aaattgattt ttacttgaga aaagagattc tccatacgac tatggtctct ccaaccggct
     7381 tgttgcagat aggaaccaaa caaaccatag ttgaaatttt cggtacgacc tcctgcatag
     7441 agctctgatt tgataagccc atactgccca aaaaccaacc gcggcgatac ttcaaacgca
     7501 tgctgcggat ttcgtgtcgt gatgtgaatc atgccaccag acgcattacc atacaaagat
     7561 gccgagggac cgcgcaatac ttccatccgc tcgatcatag caggatcgat cgcatcgagc
     7621 tgggtttgcc cgtccggtaa ggtttgagga atatcatcga cacgcatttg aataccacgc
     7681 acaccaaaag gagaacgtgc accaaaaccg cggatcgcaa tccgtagatc ctgcgtaaaa
     7741 ttaaattgat tttgaaaaaa t
//
LOCUS       DAOQKQ010000123         7751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_4586_length_7751_cov_5.402807, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000123 DAOQKQ010000000
VERSION     DAOQKQ010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 7751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7751
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4586_length_7751_cov_5.402807"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="PKZ49_08785"
     CDS             complement(<1..648)
                     /locus_tag="PKZ49_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HND36696.1"
                     /translation="MSIIERAADKLKSNADAEQKIPAIKNIHKDIIQDSKRKIQKEAS
                     DIERITEKFKNTIADSPKIQPIEPQGVDNAVKINREYIDKPISITEGRTVGKKVSVDT
                     HLPQPHIDIDLDRLHKLGIVTPQHGKTQIAEQFRIIKRPLLTNAFSKNSGIKNGNLIM
                     ITSSLAGEGKSFCSTNLAMSIAMEMDNRVLLVDADVARPSIPKMLDFSPDRKGLLD"
     gene            complement(690..2252)
                     /locus_tag="PKZ49_08790"
     CDS             complement(690..2252)
                     /locus_tag="PKZ49_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HND36697.1"
                     /translation="MDELIAQLLVYIKGTWKYRWVSIAIAWLVSIIGWTVVHHLPDDY
                     EASARIHVDTQNILRPLMAGMTVSPNPEQQVAIMSRTLISRPNVERVIRMVDLDVKVT
                     DDVAYDKLVTSLMKDIRLGAAGRENLFTISYSHKDPKLAKDIVQSLLTIFVEGGLESK
                     KQDSTSALRFLDQQIASYEEKLIASETALTGFRQRNIGLMPGQSGDYYAQLTNALEEL
                     EKAKVALKEAEQARDAVRRQISGDETVLLLETHENVLEKIINPEIDARIQALNLNLDN
                     LRLNFTDLHPDIVATKRLITQLEERKKEEATLIDPTAVDQGRNFSPMLQQLNIALTEA
                     EAVVASMQARVSEYTNRFERLKSLSSMIPTVEAELTQLSRDYNVNKANYEKLLERRAS
                     AEISGELTSTTGLMSFRIIDPPVVPETPSGPDRKKMYSFVFIGALLAGIGVAFIISQI
                     RPTFHSQTDLRQVTGLAILGTIPMVWTDQEKSKRRNRLYAFGFSLLVLMALYTTLMIY
                     TKLPVATLINLF"
     gene            complement(2302..2871)
                     /locus_tag="PKZ49_08795"
     CDS             complement(2302..2871)
                     /locus_tag="PKZ49_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HND36698.1"
                     /translation="MVFLFSACTSYPLLTNQGEKIAHDYLIGPGDTLNIIVWRNPEIS
                     MSVPVRPDGKITTPLVEDLPASGKTSTQLARDIEETLSKYLQQPVVTVIVTGFVGPFT
                     EQIRVIGEATRPQALPYSENMTLMDVMIAVGGITDFAAGNRARIIRTIDGKQQQFRVR
                     LNDLIRDGDITANTPVYQGDILVIPESFF"
     gene            complement(3377..4453)
                     /locus_tag="PKZ49_08800"
     CDS             complement(3377..4453)
                     /locus_tag="PKZ49_08800"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HND36699.1"
                     /translation="MQKIKVLVIDDSALICKILAEIINNQPDMEVAGVAHDPIVAREL
                     IRELNPDVLTLDIEMPKMDGLDFLERLMRLRPMPVLMISTLTEANSEPALLALELGAV
                     DFISKTKFDMATGLESFSDEVVSKIRMSVYAKIKKSTANQSEQSVKQNLSYGTNPTNW
                     GNKLIIVGASTGGTEAIREFLMELPPDVPGILIAQHMPESFTKPFANRLNTQCRITVV
                     EAQGGERVLPGHAYVAPGHSHLLLKRSGGYYMTELSQSEPVNRFRPSVDVLFRSAATH
                     AGKDALGVIMTGMGKDGAAGMLEMHRAGAYTFAQDEESCVIFGMPREAIAMGGVDEIV
                     PLKNMARQVLRRLAYSDKPNQLRA"
     gene            complement(4513..5100)
                     /gene="cheD"
                     /locus_tag="PKZ49_08805"
     CDS             complement(4513..5100)
                     /gene="cheD"
                     /locus_tag="PKZ49_08805"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF010013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemoreceptor glutamine deamidase CheD"
                     /protein_id="HND36700.1"
                     /translation="MDDGITYQYAKYSYFNCKLNREVIKLLPGEFYVSDNDVVMETVL
                     GSCIAACVYDSNQGIGGMNHFLLPAPPTQDREKDRSSARYGEYAMDILISEVIRMGAI
                     RSNLEVKLFGGGNVQHSMTAMNIGGKNADFALDYVKRKEIHLAGYDLVDIYPRKIIFY
                     PKDGLVMVKKLMIEPASIINDEKNYESILKNQIDK"
     gene            complement(5170..>7751)
                     /locus_tag="PKZ49_08810"
     CDS             complement(5170..>7751)
                     /locus_tag="PKZ49_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HND36701.1"
                     /translation="TELANHFSKQAEIYMQARNQFLNMIGNSDFVAARNYLEQETDPK
                     LSQAFLALQNLQALQETIAQSEYSDAGIALKSISSAITIAVLFIACLLIYMGINLIRA
                     VIKPISDISQVIKRVAEGDFSTQLEVRNFEEIGLLTEEIKMMQNRSGFEFNEAKRVAS
                     ESLRIKNALDQSNSSIMIADSNGQIIYLNQAAEALFKTNEVGFAKELPEFKAGNLLGA
                     SFNLFNQKMNLMSITQSQKVDTVLGGRIFSLVFVPVINEQQQRLGTAIEWIDRTQEIA
                     VEKEVSGVVSAAVDGDFKKRLTLEGKSGFFKHLSEAINKLMETTSTGLADVTRVLDGL
                     AHGDLSQKIEGDYKGTMGQLKDSANQTVTQLNEIVSQIKMMVAGAAAGDFSQRGDVTQ
                     YQNEFKEMIKGLNQLMQICESGLKDMVRVLNALAKGDVSQKIEGDYKGTFDQLKSSSN
                     QTIAQLNEIVSQIKIMVAGAAAGDFSQRGDVTHYQNEFKEMIEGLNQLMQICELGLKD
                     MVRILNALAKGDLTQKMTNEYEGIFGQLRDDSEQTVTQLTVIVNKIRESVESINTASR
                     DIADGNNDLSQRTEEQAANLQETASSIEELTSTVKQNAENAKHANELAASASGVAIKG
                     GEVVGRVVHTMSSINDSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAV
                     VATEVRALAQRSAAAAKEIKELISDSVNKVEDGTQLVDQAGKTMDEIVTSVKHVTEIM
                     AQIASASQEQSEGIEQVNDSITKMDEITQQNASLVEEAAAASESMREQAEQLAQAVRV
                     FKLAQGSAPNQSVQKVERRGPDRAKNVERIHSSSAADKAKKGRYVLPDPAKSGTDDDW
                     EAF"
BASE COUNT         2169 a         1815 c         1288 g         2479 t
ORIGIN      
        1 atctaataat cctttacgat ctggactaaa atccagcatt tttggaattg aggggcgtgc
       61 tacatcagca tcaaccaata aaactctatt atccatttcc attgcgatac tcatcgctaa
      121 atttgtagaa cagaagctct taccctcacc tgccaacgaa ctggtgatca ttatcagatt
      181 accgttcttt attccagaat ttttactaaa cgcgtttgtt aacagtggac gcttaataat
      241 acgaaactgt tcagcgattt gggtttttcc atgttgtggc gtcacaatac ccaatttatg
      301 caatctgtct agatcaatat ctatgtgagg ctgtggcaga tgagtatcaa cacttacttt
      361 cttacctaca gttcttccct cagtaataga tatcggttta tcaatatact ctcggtttat
      421 cttaacagca ttatctaccc cttgtggttc aataggctgt attttcggcg aatcagctat
      481 tgtattctta aatttttctg taattctttc aatatctgag gcctcttttt gaatctttct
      541 tttagagtct tgaattatat ctttatgaat attcttaatt gccggtattt tctgttctgc
      601 atcagcatta ctctttagct tgtcagcagc cctttctata atactcatat caattcctca
      661 ctaaaaatac ctggattcgc aaacaacgtc taaaagagat taattaatgt tgcaactgga
      721 agttttgtgt aaatcattaa ggtagtatat aaagccatca gtaccaatag agaaaaaccg
      781 aatgcataga gtcgatttct tcgcttagat ttttcttgat cggtccaaac cattggaatg
      841 gtgccaagaa tagcaagacc agtaacttgt cgcaaatcag tctgactatg gaatgttgga
      901 cgaatttgac taattataaa agcaacgcca ataccagcta gcaacgcgcc aataaaaaca
      961 aacgagtaca ttttctttcg atctggccct gatggagtct caggaacaac aggcgggtct
     1021 ataatcctaa atgacattaa acccgttgta gaagtcagtt cgcctgatat ctcagcagag
     1081 gccctgcgtt caagtaattt ctcataatta gctttattaa cattataatc ccgacttaat
     1141 tgagtaagtt ctgcctcaac tgttggaatc atagaactca gcgatttaag tcgctcaaaa
     1201 cgattcgtgt attcagatac ccgtgcttgc attgaagcga ccaccgcctc agcttcagtc
     1261 aatgcaatat tcaattgctg tagcatggga ctaaaattcc ttccttgatc taccgcagtt
     1321 ggatctatca gcgttgcctc ttctttttta cgttcttcga gttgagtaat taaacgttta
     1381 gttgccacaa tgtcgggatg taaatctgta aaattgagtc taagattatc aagattcaga
     1441 ttcagcgcct gaatacgtgc atcaatttca gggttaatta ttttttctaa aacattttca
     1501 tgtgtttcaa ggagtaaaac cgtttcatca ccacttattt gccttcttac agcgtctctt
     1561 gcttgttcag cttcttttaa tgccactttt gctttctcaa gctcttctag ggcatttgtt
     1621 aactgcgcgt aataatcccc actttgacct ggcattaggc cgatatttct ttgcctaaac
     1681 cctgtcaatg cagtttcaga agcgatgagt ttttcttcat aactcgctat ttgttgatct
     1741 aaaaaacgta atgctgaagt cgaatcctgt tttttacttt ctaaaccacc ttcaacaaaa
     1801 atagttagta atgactgcac aatatcttta gctaattttg gatctttatg gctatatgaa
     1861 atagtaaaaa gattttccct accggctgca cccaatctaa tatctttcat taatgaagta
     1921 acaagcttgt cgtatgcgac atcatcagta accttcacat ccagatcaac cattctgatc
     1981 acccgttcaa cattgggacg actgatcaaa gttcgactca tgatcgcaac ttgctgctct
     2041 ggattgggtg agacagtcat acccgccatc aggggcctaa gaatattttg agtgtccaca
     2101 tgaattcgcg cggaagcttc ataatcatca ggtaagtgat gaacaaccgt ccaaccgata
     2161 atagatacaa gccatgcgat tgcgatggaa acccaacggt acttccaggt tcctttaata
     2221 taaacaagca gttgagcaat taactcatcc atcttgcatc aatctccatt tctataaaaa
     2281 tcttaacaga tgctgttatg attaaaaaaa actctcagga atcaccaaaa tatcaccttg
     2341 atatacagga gtattagcag ttatatcccc atccctgatc aagtcattta gcctaactct
     2401 gaattgttgc tgtttaccat ctatggttcg aataattctt gcacgatttc ctgctgcaaa
     2461 atctgtgatt ccaccaactg caatcattac atccattagt gtcatatttt cgctgtaagg
     2521 caaagcttgg ggtcgcgttg cttcaccgat tactctaatt tgctcagtaa atggccctac
     2581 aaatccagtt acaatcactg tgacgactgg ctgctgcaaa tattttgaaa gtgtttcttc
     2641 tatatctctg gctaattgag ttgaagtctt cccacttgcc ggcaaatctt caactaaagg
     2701 ggttgttatt ttcccatcag ggcgaaccgg aacagacatt gatatttcag gatttctcca
     2761 aacgatgata ttcaaagtgt ccccaggccc gattaaataa tcatgtgcaa tcttctctcc
     2821 ctgattcgtt aggagaggat agctagtaca tgcgctaaat aaaaatacca aacatacaat
     2881 taaaactgct atggatcttc ttaaaaattg atcgttattt gggatatcca ctttatgcct
     2941 cattaaaaaa atcgctaaaa gaatttagaa cattccatat cacttggatg ttaaaccaaa
     3001 aaaaaaaatt caaacaccat aaaatggatg aatttgatgg catttctgaa gataagatgc
     3061 tgatgacgca acttatttac tattaaagaa atttgcaaac agtatcagga cttgccagtt
     3121 acattaatac tatattgtgg aactaattcg ttgcaagcta cccgaaattt tggaataact
     3181 cgagtcaata tgaatttaaa aatcgacttc cttagttttt ctggaaatta ggctatatct
     3241 gtagtctcat tttaaccaga ttcatgcggt taattatcat atatcaacaa aacttagttt
     3301 tttttgtaac attattacaa cgctttatta caatacgtta tcaaatcatc cgcagcaata
     3361 attcaggcca ctcaacctaa gcacgaagct gattgggctt atcagaataa gcgagtcttc
     3421 tcagaacttg cctagccatg tttttaagcg gaacaatttc atcaacgcct cccattgcaa
     3481 tcgcttctct tggcataccg aatattacgc aactctcttc atcttgtgca aaggtataag
     3541 cgcccgctcg atgcatctct agcatacctg cggcaccatc cttacccata cccgtcatga
     3601 taacccctaa tgcatcttta cctgcgtgag tggcggcgga tctaaataaa acatcgactg
     3661 aagggcgaaa acgattcacc ggctcagatt gactaagctc agtcatataa tatccaccgc
     3721 ttcgtttgag tagaaggtga gagtggcctg gcgctacata agcatgccca ggtaataccc
     3781 gctcacctcc ttgggcttcg acaacagtaa ttctgcattg cgtattcaaa cgatttgcaa
     3841 atggctttgt aaaggattcg ggcatatgtt gtgcaattaa aatgcctgga acatcaggcg
     3901 gtaattccat taagaattct ctaatagcct cagttcctcc agtagacgcg cccactataa
     3961 tgagcttatt tccccaattt gttgggttgg ttccgtaaga aagattttgt ttcacggatt
     4021 gttcagattg attagcagta gatttcttaa tttttgcata tacactcata cgaattttgc
     4081 tgaccacttc atcactaaag ctctccaagc ccgtagccat atcaaattta gtttttgaaa
     4141 taaaatcaac agctccaagc tcaagcgcta ataaagccgg ttcagaatta gcttctgtca
     4201 atgttgagat catcaatacc ggcatgggcc gcaaacgcat aagcctttca agaaaatcta
     4261 aaccatccat tttaggcatt tcaatatcga gcgtgagtac atctggatta agttctcgaa
     4321 tgagctcccg tgcaacaatc ggatcatgag ctacgcccgc cacttccata tcaggttgat
     4381 tattaataat ttctgccaaa attttacaaa ttagtgctga atcatcaata actaatacct
     4441 tgattttttg catctactca cctctttatc aatatttgct tgatttttaa tttcatgatt
     4501 cagccttatc aatcatttgt ctatctggtt tttcaaaatt gactcatagt tcttttcatc
     4561 attgataatt gaagctggtt caatcattag tttcttaacc attacaagtc catcctttgg
     4621 atagaaaata attttgcgtg gataaatatc gactaaatcg taacctgcta agtgaatttc
     4681 tttccgtttc acataatcta atgcaaaatc agcgtttttc cccccgatgt tcattgcggt
     4741 catactgtgc tgcacattgc cgccgccaaa caacttaacc tccaggttgc tgcgtatcgc
     4801 ccccattctg atcacttcac taataagaat atccatagcg tactctccat aacgtgcaga
     4861 agatcgatct ttctcccgat cttgggtagg tggcgcaggc aacaagaagt gattcatgcc
     4921 accgataccc tggtttgaat cataaacaca ggcagcaata caagaaccca atacagtttc
     4981 cattactaca tcgttatcac tcacataaaa ctcaccaggt aatagcttta tgacttcacg
     5041 attaagtttg caattaaagt aactatattt tgcatattga taagttattc cgtcatccat
     5101 ggccattcga attatttcta tgaatattca taaccgttag taacaagatg acactgatct
     5161 cgcacaaaac tagaatgctt cccaatcgtc atcggtacca gatttagcag gatcaggtaa
     5221 tacataccgc cctttcttag ctttatcagc tgcgctcgat gaatgaattc gctctacatt
     5281 cttcgcacga tccggtccac gtcgctctac cttttgtacg ctttgattag gcgctgatcc
     5341 ttgagctagc ttgaatactc ttactgcctg tgcaagttgt tctgcttgtt ctcgcatcga
     5401 ttccgatgcc gctgccgctt cctcaactag cgatgcattc tgttgagtga tctcgtccat
     5461 cttcgtgatc gaatcgttta cttgctcaat accctcactt tgctcctgcg atgccgatgc
     5521 aatctgcgcc ataatttctg tcacgtgctt cactgaagtc acgatctcat ccattgtctt
     5581 acctgcctga tctaccagct gagttccatc ttcaacctta ttaaccgagt cactaatcaa
     5641 ttctttgatt tccttagctg cagccgctga gcgttgcgcc agcgctcgca cctccgttgc
     5701 cactaccgcg aaaccacgcc cctgttcgcc tgctctggct gcctcaacag cagcattcaa
     5761 tgccaaaata ttggtctgaa aggcaatgcc gtcaatcacg ctgataatgt cagcaatctt
     5821 cttcgagcta tcattgattg agctcatggt atgcacaact cgcccgacca cttcaccacc
     5881 cttaatcgcc acgccagagg ctgatgctgc caactcattt gcatgttttg cattctccgc
     5941 attctgtttt accgttgagg tcaattcctc tatgctcgat gctgtctctt gcagattcgc
     6001 tgcttgctct tccgtacgtt gtgatagatc attgttccca tctgctatgt ccctggatgc
     6061 cgtattgatg gattcaactg attctctgat cttattcaca atcaccgtca attgcgttac
     6121 cgtctgttct gaatcatctc gcaattgtcc aaatatccct tcgtactcgt tcgtcatctt
     6181 ttgcgtcaaa tcacccttcg ctaacgcatt caatatcctt accatatctt ttaaacccaa
     6241 ttcacaaatc tgcatcagct gattcaatcc ttcgatcatt tccttgaatt cattctgata
     6301 atgggtaaca tctcctcgct gactaaaatc tcccgcagcc gcacccgcta ccattatctt
     6361 gatctgcgat acaatttcat tgagctgcgc tatagtttgg tttgagctac tctttaattg
     6421 atcaaacgtg cctttgtaat ccccttcaat cttctgtgac acatctccct tcgctaatgc
     6481 gtttagcact ctcaccatat cttttaaacc cgattcacaa atttgcatca gctgattcaa
     6541 tcctttgatc atttccttga attcgttctg atactgagta acgtctcctc gctgactaaa
     6601 atctcctgca gccgcacccg ctaccatcat cttgatctgc gatacaattt cgtttaactg
     6661 cgttactgtc tgatttgcgc tatctttcaa ttggcccatt gttcctttgt aatcaccttc
     6721 gatcttttga gataaatcgc catgtgccaa cccgtctaaa acacgtgtaa catctgcaag
     6781 tcctgtagaa gtcgtttcca tcaacttatt gattgcctca gataaatgtt tgaagaaacc
     6841 tgactttcct tcaagggtca aacgtttctt aaaatcacca tcgaccgcgg cagatacaac
     6901 accggatacc tctttttcca ccgcaatttc ctgagtacga tcgatccact ctatcgcagt
     6961 acctaaacgt tgctgttgct cattgatcac tggaacaaaa actaacgaaa aaattctccc
     7021 gccaaggaca gtatcaacct tctggctttg ggtaatactc attaaattca ttttctgatt
     7081 aaacagatta aagcttgcgc caagcaaatt cccggcttta aattcaggta gttctttagc
     7141 aaatccaact tcatttgttt taaatagcgc ctcagcagcc tgattcaaat aaataatttg
     7201 accattggaa tcggcaatca tgatcgagga gttcgattgg tctagtgcat tcttgatgcg
     7261 taagctttct gaagccactc gttttgcttc attaaactca aatccagatc ggttctgcat
     7321 catctttatc tcttcagtca aaagcccaat ttcctcgaag ttacgcactt caagctgagt
     7381 actaaaatca ccttcagcca ctcgtttaat tacttgacta atgtcactaa taggttttat
     7441 cactgcgcgt attaggttaa tccccatata aatgagtaga caagcaataa acagaaccgc
     7501 aattgtgata gcactagaaa ttgattttag ggctattccc gcatcactat attctgactg
     7561 cgcgatcgtt tcctgtaacg cttgcaaatt ctgtaacgct aaaaaagctt gactaagctt
     7621 tggatcagtt tcttgttcca aataatttct tgctgctaca aaatcactat tcccaatcat
     7681 attcaaaaat tggttacggg cctgcatata aatttcagct tgtttagaaa aatgatttgc
     7741 taattcagtt t
//
LOCUS       DAOQKQ010000124         7729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_4606_length_7729_cov_5.001824, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000124 DAOQKQ010000000
VERSION     DAOQKQ010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 7729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7729
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4606_length_7729_cov_5.001824"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="PKZ49_08815"
     CDS             <1..842
                     /locus_tag="PKZ49_08815"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HND36702.1"
                     /translation="DAFLFDWSIYQVADKAIKMSYSGTHWDRLQLRSRIESARTQLPY
                     LEKAAINIEPGEYPAYLSAAAVNELLGLLNWSGFSAKEQHTQQSVLQALVNKERTLSP
                     HITLRENTQLGLAPAFDSVGFVKSDQITLIDAGQHASSLISPRTAKEYGLQANGANES
                     ETTQSLELLPGTLDSTNLLEQLDEGIYVSNLHYLNFSDRTACRMTGLTRFATFWVEQG
                     KIVAPLNVMRFDDSLYRMLGSHLMGLTRETEWLLSSDTYGQRSVGSSRVPGALLSKLR
                     LTL"
     gene            complement(1264..2610)
                     /locus_tag="PKZ49_08820"
     CDS             complement(1264..2610)
                     /locus_tag="PKZ49_08820"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HND36703.1"
                     /translation="MIRELQQFKIALDAHAIVAITDYKGKICYVNNKFCEISKYSAEE
                     LVGKDHRIINSGYHSKEFFRNLWDTIKSGKIWKGEIRNRAKDNSIYWVDTTIVPFLDD
                     EGKPYQFIAIRAEVTLRKQLEDEILAKVAWQTAILSYAGHAMIATNPEGVIQTFNPTA
                     EDMLGYKAEELVGKATPAVFHEPNEVIDRAAKLSTELGIAIEPGFEVFVARARKNHAY
                     EDEWTYIHKDGTHFPVRLIVTAIKNFNEIIGFLGIAQNITEQKDILNKTKFLATHDEL
                     TGLANRYLLYDRIQQLLSDIERNHGSAAMLMIDLNDFKKINDTLGHDIGDLLLKEVSK
                     RLTSTLRAIDTVARLGGDEFVVVTPHVSVAGIKLVVQKIIHLLPYPIFIANNEMKIGV
                     SIGITLIPSEGKDTKTLLKNCDIAMYRAKKMGRNNYQFFSGELSNEVTVLNPKTLD"
     gene            2938..4113
                     /locus_tag="PKZ49_08825"
     CDS             2938..4113
                     /locus_tag="PKZ49_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HND36704.1"
                     /translation="MVTESFDVVIVGGGPVGMALALRLREWNIATLLLEAQPLLTNPV
                     TDSRPLALSYGSQLILKRLDVWDNLVSVTPIQTIHISNHGHAGQTLIQSEDAGVPVLG
                     SVVNYHGLYDALYQMMRQQNVTFQLGAKVTSLIHQPDHVQVDYEQSGETHRVDAKLLV
                     LADGGKLASQVEGIHFHTHDYQQWAVVANIQTSIDQRGIAYEHFTLDGPVALLPSGTG
                     YALVWTVPAEQVDRILNWDDATFLSELQVHFGDMAGKFVSCGKRTGFPLTLKYASPTI
                     APRTVLIGNASQTLHPVAGQGFNLGLRDAWELASELIRSANFSADLGSSRLLSSFASK
                     RQIDREGGKFFTNTLAKLFTVDLTSLQVACGLGLSILDQLSPVKRFVARRMIFGARG"
     gene            4183..5196
                     /gene="dusB"
                     /locus_tag="PKZ49_08830"
     CDS             4183..5196
                     /gene="dusB"
                     /locus_tag="PKZ49_08830"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HND36705.1"
                     /translation="MQIGPYRLKNQLIVAPMAGVTDRPFRQLCKRFGAGMAVSEMVSS
                     NSLLWGSEKTRRRASHEGEVAPISVQIAGADPTMMAEAARYNADQGAQIIDINMGCPA
                     KKICNVMAGSALLKDETLVGKILEAVTTAVDIPVTLKIRTGWDTQHKNALSVAKLAEQ
                     AGIQALAIHGRTRACAYQGYAEYDTIAAVKAAINIPVIANGDITTPEKAKQVLDYTKA
                     DAVMIGRAAQGRPWIFREIDYYLNTQKFLPLPEVSEIHQVLIEHLHDLYEFYGEYSGV
                     RIARKHISWYTKGLTNSAVFRHAMNQLQTVEAQLTATHQFFQQLADYGQRLTYDNEVA
                     IAV"
     gene            5193..5435
                     /locus_tag="PKZ49_08835"
     CDS             5193..5435
                     /locus_tag="PKZ49_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HND36706.1"
                     /translation="MSSFNENEIASCIRKSLDAYFKDLDGEKPCPIYEMVINSVEKPL
                     LELAIQYAKGNQSKAAELLGISRNTLRSRLAKHRIE"
     gene            5442..6998
                     /gene="purH"
                     /locus_tag="PKZ49_08840"
     CDS             5442..6998
                     /gene="purH"
                     /locus_tag="PKZ49_08840"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010393318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HND36707.1"
                     /translation="MIKQALISVSNKVGVVDLARALHQRGIKIISSGGTAKLLQENAI
                     PVTEVSDYTGFPEMLDGRVKTLHPKIHAGILSRMDLDAHRQAIQQVGIDPIELVVVNL
                     YPFVETINRAGCTLADAIENIDIGGPTLIRAAAKNYQHVTVITEPEDYSVLLAELDHG
                     SGAVSDSRRFYFAQKAFTHTARYDSAISNYLTAIDADGQHTTFPEHLNLNFSRVQTMR
                     YGENPHQQAVFYRDASLLPGSLANYTQLQGKELSFNNIADTDAAWECVKSFDKPACVI
                     VKHANPCGVAIADNLLAAYQFAFATDPTSAFGGIIALNQRVDAALAEAVIKQFVEVII
                     APAFSEEAQAVLKQKANVRVLVVPIQAESNRYELKRVGGGLLVQTPDDFHVGEADLKV
                     VTQLKPTPEQIQDCLFAWRVAKFVKSNAIVFCAQGRTLGIGAGQMSRVDSARIASIKA
                     EQAGLGLNGSTVASDAFFPFRDGLDVVVKAGAKIVIQPGGSIRDDEVIQAADEQGVIM
                     LFTGIRHFRH"
     gene            7087..>7729
                     /locus_tag="PKZ49_08845"
     CDS             7087..>7729
                     /locus_tag="PKZ49_08845"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413082.1"
                     /note="catalyzes the formation of
                     N(1)-(5-phospho-D-ribosyl)glycinamide from
                     5-phospho-D-ribosylamine and glycine in purine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HND36708.1"
                     /translation="MKLLVVGSGGREHAIAWKLSQSPRVQKVFVAPGNAGTALEAGLE
                     NIPITAIAELVAFVKQENIALTVVGPEAPLAEGIVDIFRAEGLRIFGPTQKAAQLEAS
                     KIFAKAFMQCYGIPTANSASFNEADAAHRYINQGAVPIVIKADGLAAGKGVVVAQTYE
                     QAHEAVDRMLTSSQLGKAGAQILIEDFLEGEEASFIVMADGQHALPLATSQDHK"
BASE COUNT         2125 a         1522 c         1814 g         2268 t
ORIGIN      
        1 tcgatgcttt cttgttcgat tggtctattt atcaggttgc ggataaagcg atcaaaatga
       61 gctactcagg aacgcattgg gatcgattgc aactaaggag ccgaattgaa tcagcgcgca
      121 cgcagctacc gtatctcgag aaagctgcaa ttaacattga acctggagaa tatccagcct
      181 atctgagcgc tgctgccgtc aatgagttgt tagggttgct gaattggagc ggtttttccg
      241 ccaaggagca acatacgcag caaagcgttt tgcaagcctt ggtgaataaa gagcgaacgc
      301 tttccccgca catcacgcta cgagaaaata ctcagcttgg cttggcacct gcttttgata
      361 gtgttggctt tgtaaaatca gaccagatta cgttgatcga tgccggacag catgcgagta
      421 gtttaatatc gccacgcacc gccaaagaat atggattaca agccaatggc gccaatgaaa
      481 gcgaaacgac gcagtcgctc gaactgctac caggcacgct tgatagcacc aacctacttg
      541 aacagctcga tgaaggcatc tatgtcagca atctgcatta tctcaatttc tcagatcgta
      601 ccgcttgccg catgacgggg ctaacgcgtt ttgcaacttt ctgggttgaa caaggaaaaa
      661 ttgtagcgcc gctcaatgtc atgcgtttcg atgacagcct ttatcggatg ttaggtagtc
      721 atctcatggg attgacacga gaaacggaat ggttgttaag ctcagatact tatggtcagc
      781 gatcagtagg ctccagccgt gttcctggtg cgttattatc gaagctgcgg ttgacgctgt
      841 gaatacatag atctgtaaca gtcgataatc aaaaaatatc taggagtaga attcctcgtc
      901 acgaagtacg aggaatatac actgacatgc ttatttacga aaagtacatc gatatcagaa
      961 ttaaagatgc tgccaccaac cctataatgg ctatattaat gcccaattta gtcatcgtca
     1021 tagaatcttc tttctctagc atttccatca ttacctccta aaatcaatga ttaacggaaa
     1081 ttgaattttc cacctaatca ataggttagt cgttgatcaa gatcaataaa gtcaatcaaa
     1141 ttttttgatt tagatcaaaa aatgaaaact aggcgggggt gctcatctaa ggagcaaaac
     1201 taatttaaag tgatttttat gattaaactg gtatgaaagt actagcatga tagatgtact
     1261 cgactaatct aaagttttag gattgagtac agtaacttcg ttgctgagtt cccctgaaaa
     1321 gaactgataa ttgttacgtc ccatcttttt tgcacgatac attgcaatat cacaattttt
     1381 taatagtgtt ttggtatcct ttccttctga tgggattagc gttataccaa tgcttacacc
     1441 aattttcatt tcattattag ctataaatat tggatacggt agaagatgta tgattttctg
     1501 tacaactaat tttattcctg cgactgaaac atgtggcgtt actactacaa actcatcacc
     1561 acctaagcgc gcaactgtat ctattgcgcg caaagtagat gttaatcttt tactgacttc
     1621 tttgagcaag aggtcaccaa tatcatgtcc taatgtatcg ttgatttttt tgaaatcatt
     1681 cagatcgatc atcaacatgg cagccgatcc gtggtttcgt tcgatatcag atagcaactg
     1741 ctgtatacga tcgtagagta aatatcgatt agcgagccct gttaactcat catgggttgc
     1801 caggaatttt gtcttattta atatatcttt ctgctcggta atgttttgtg caattcccag
     1861 aaagccaatt atttcattaa aattttttat agcggttact atcaaacgaa cagggaagtg
     1921 ggttccatct ttgtggatat aggtccattc atcttcataa gcatgatttt ttcgtgcacg
     1981 agcaacaaac acttcaaatc caggttcaat tgcaattccc agctctgtag ataatttagc
     2041 tgcacgatca attacttcat tcggttcatg aaaaacggct ggggttgctt tgccaaccaa
     2101 ttcctctgct ttataaccaa gcatatcttc agccgtggga ttgaatgttt ggataacgcc
     2161 ttctggatta gtagctatca tggcatggcc agcgtaactc aatattgcag tctgccaagc
     2221 tacttttgct aaaatttcat cctctaactg tttgcgcagt gttacttcag cacgtatagc
     2281 gatgaactgg taaggtttgc cttcatcgtc gagaaaaggc acgatggttg tatcaaccca
     2341 atagatcgaa ttgtctttag cacggttcct aatttcacct ttccagattt ttccactttt
     2401 gattgtgtcc cataaatttc tgaaaaattc tttcgagtgg taacctgaat taataattct
     2461 gtgatctttc ccgactaatt cttcagcaga atattttgag atttcacaaa atttgttatt
     2521 cacataacaa attttgcctt tgtaatcagt aattgcaacg atcgcatgcg catcaagtgc
     2581 tattttgaat tgctggagtt ctctaatcat gattctatgt gtaaaaactt atgagcctac
     2641 actggaatat aattaaaact agaatagcaa ctgtcaaatt aatattttgt gatgtggttt
     2701 ttgttttcta acgcgttgag aatgatttca gatttatcag gaatatttga atttttcggt
     2761 aaatctctgc gatgaattta tcagagcaca gatttgatta ggcaggcgtt ttgtctaaag
     2821 tatgagttgt gatattctgc ttggccttag tgagaagtat gaaatttact ccggaaagaa
     2881 aatttttgac tagactgatt tgagtgatga tcgattctat aaataatagg cttagttatc
     2941 gtgactgaat cgttcgatgt ggttattgtc ggcggtgggc cagttgggat ggcgctggca
     3001 ttgcgcttga gagagtggaa tatcgcgacg ctgttgctgg aagcgcaacc cttattaacc
     3061 aatcctgtta ccgattctcg acccttggcg ttgtcgtatg ggagccagct cattttaaag
     3121 cggttagatg tatgggataa cctagtgtcg gttaccccga ttcagacgat tcatatttcg
     3181 aatcacggtc atgcgggaca aacgttgata caatctgaag atgccggggt tccagtgctc
     3241 ggtagtgtcg ttaattacca tgggctgtat gatgcccttt atcagatgat gcgtcagcag
     3301 aatgttacgt ttcaattggg cgccaaagtg acttcattga tccatcaacc ggatcatgtg
     3361 caggttgatt atgaacaatc gggagaaacg catcgcgtgg atgctaaatt gctggtgctc
     3421 gccgatggcg gtaaactggc ttcacaggtg gaaggaattc actttcatac gcatgattac
     3481 cagcaatggg ctgtggttgc caatattcaa accagtattg atcagcgtgg aattgcctac
     3541 gagcatttta ccttggatgg tcctgttgct ttgttgccat ctggtacggg ttatgcgtta
     3601 gtatggactg tgccagccga gcaggtcgat cgcattttaa attgggatga cgcaactttt
     3661 ctttctgagt tgcaggtaca ttttggtgat atggcgggca aattcgtttc atgtggcaag
     3721 cgcacgggat ttccactgac actgaaatat gcatcaccta cgattgcccc tcggaccgta
     3781 ttgattggca atgcatcgca aacgttacat ccggttgcag ggcagggctt taatctggga
     3841 ttacgcgatg cttgggaatt ggcaagtgaa ttgatccggt ctgctaattt ttcagcggat
     3901 ttgggttctt cgcggctgct gtcatcgttt gcgagtaaac gtcagattga tcgagaagga
     3961 ggtaaatttt ttaccaatac acttgcaaaa ttgttcacgg tggatttgac ttctttgcaa
     4021 gttgcgtgtg gattgggttt gagtattcta gatcagctat ctcctgttaa gcgttttgtt
     4081 gcacgtcgta tgatatttgg tgcaagagga taattgagtg acgatcatca gtgtggaatt
     4141 aatcagaatt ctttgaaata aaccagattg acataacttc gtatgcaaat cggcccttat
     4201 cgtttaaaaa atcagttaat cgttgcgccc atggcaggtg tgacggatcg cccttttcgt
     4261 caattgtgta aacgatttgg cgcgggtatg gcggtatctg agatggtttc tagtaattcg
     4321 ttgctttggg gttctgagaa aacacgtcgt cgggccagcc atgaggggga ggttgcgccg
     4381 atttcggtac aaatcgctgg cgcagatcca acaatgatgg ctgaagcagc acgctacaat
     4441 gccgatcaag gggcgcaaat tatcgatatc aatatgggtt gtccggccaa aaagatttgc
     4501 aatgtgatgg cgggatcggc tttattaaaa gatgaaacat tggtagggaa aattttggag
     4561 gcggttacta ccgccgttga tattccagtg acgctaaaaa ttcgcacggg ctgggacacg
     4621 caacataaaa atgcgctgtc agtcgctaag ctagctgaac aagcgggtat ccaagcgcta
     4681 gcgattcatg gcagaacacg tgcttgtgcc tatcaagggt atgcagagta tgacaccatt
     4741 gccgcagtga aggctgcgat taatatccct gttatcgcaa atggggatat tacgacaccg
     4801 gaaaaggcca agcaggttct agattataca aaagccgatg ccgttatgat tgggcgtgct
     4861 gcacaaggac gcccctggat ttttcgcgaa attgattatt atttgaatac ccagaaattt
     4921 ttacccttgc ccgaagtgag tgagattcat caagtgctca ttgaacattt gcatgatctt
     4981 tacgaatttt atggtgaata ttcgggtgtt cgcattgcac gtaaacatat ctcttggtat
     5041 accaagggct taaccaattc agccgttttt cggcatgcaa tgaatcaatt acaaacggtg
     5101 gaagcgcaat tgactgcgac gcatcaattt tttcaacaat tagctgacta tgggcaaagg
     5161 cttacttatg ataacgaggt ggcaatcgca gtatgagcag ttttaatgaa aatgagatcg
     5221 caagctgtat tcgtaagtcc ctggatgctt atttcaaaga tttagacggt gaaaagcctt
     5281 gccctatcta tgaaatggtt atcaacagtg tcgaaaaacc gttacttgaa ctggcgattc
     5341 agtatgcaaa aggtaatcaa agtaaggcag cagaactttt aggaataagt cgtaatacgt
     5401 tgcgcagccg cctggctaaa catcgtatcg aatgatacat tatgattaaa caagcattaa
     5461 ttagtgtttc aaataaagta ggtgtggttg atttggcgcg agcattacat cagcgcggaa
     5521 taaagatcat atcatccggt gggaccgcaa aactgttgca agaaaatgct attcccgtta
     5581 ctgaagtcag tgattatacc gggtttcccg aaatgctcga tggccgggtc aagaccttac
     5641 atcccaagat tcatgcaggt atattatcca ggatggacct tgatgcccat cggcaagcga
     5701 tacaacaagt tgggatcgat ccaattgaat tagtggttgt taatctctat ccttttgttg
     5761 aaacgattaa tcgtgcgggt tgtaccttgg cagatgcgat tgaaaatatc gatatcgggg
     5821 gaccaacctt aatcagagct gctgcaaaaa attatcagca cgtaactgta atcacggaac
     5881 ccgaagatta ctcggtttta ttagcagaat tagatcatgg aagcggagcc gtgagcgatt
     5941 cacgccgttt ctattttgct caaaaggcat ttactcatac tgcacgttat gatagtgcaa
     6001 tcagtaatta tttgacggcc atcgatgctg atgggcagca tacgactttc cctgagcatt
     6061 tgaatctcaa ttttagccga gttcaaacga tgcgatacgg cgagaatcca catcagcagg
     6121 cggtttttta ccgtgatgca agcttattac cagggagtct ggcgaactat acgcaattac
     6181 agggcaaaga attatcgttt aataatattg cggataccga tgcggcttgg gaatgtgtca
     6241 aatcttttga taagccagcg tgtgtgatcg ttaagcatgc taatccttgt ggtgtggcga
     6301 ttgcggataa tttactagca gcttatcaat tcgcatttgc aacggatcct acttcggcat
     6361 ttggtggcat tatcgccctt aatcagcgtg ttgacgctgc tttagccgaa gcggttatta
     6421 aacaattcgt ggaagtcatt attgcccctg cgtttagcga ggaagcgcaa gccgtactca
     6481 agcaaaaagc gaacgttcgt gtgctggtcg tgcccattca ggcagaatcg aatcgctacg
     6541 agctgaaacg cgttggaggc gggttattag ttcaaacacc cgatgatttt catgtcgggg
     6601 aggctgatct caaagttgtg acgcaattga aaccaacacc tgaacagatt caagattgct
     6661 tatttgcttg gcgggttgcg aaatttgtga aatcaaacgc cattgtattt tgtgcacaag
     6721 gtcggacgct gggtattggt gcaggtcaaa tgagtcgggt cgatagtgct cgcattgcat
     6781 ccatcaaagc ggagcaggcg ggtttaggtt tgaatggctc aaccgttgca tccgatgcat
     6841 tttttccatt tcgagacggt ttggatgtcg tggttaaagc aggggctaag attgtcatac
     6901 aacccggtgg tagcattcgt gatgatgaag tgattcaagc agcagatgag caaggggtta
     6961 ttatgctttt tacaggaatt cgccatttta ggcattaaaa taaccgaaac gtcgcgataa
     7021 tagattactt tttgcttaat cggtcattac attaactatt cttttctaag ctggtagtgt
     7081 ataacaatga agttacttgt agtcggtagt ggaggtagag agcacgcaat cgcgtggaag
     7141 ctgagtcaat cgccacgggt tcagaaagtg tttgttgcgc ccggtaatgc aggtacagcg
     7201 cttgaagcgg ggctcgaaaa tattccaatt acagccattg ctgagctagt ggcatttgta
     7261 aagcaggaaa atattgccct aaccgtagta ggtcctgaag cgcccttggc tgaaggaatt
     7321 gtcgatatct tccgagcgga agggctccgt attttcggtc caacccaaaa agcggcacaa
     7381 cttgaagctt caaaaatctt tgcaaaagct tttatgcagt gctatggcat ccccactgcg
     7441 aattcagcaa gttttaatga ggctgatgcg gcacatcgtt atattaatca aggtgcagta
     7501 ccgatcgtta tcaaagcaga cgggttagcc gcagggaaag gtgtcgtcgt cgcacagact
     7561 tacgagcaag cgcatgaggc cgttgatcgc atgttaacga gtagtcaact gggaaaagct
     7621 ggcgcacaaa ttttaattga agattttctg gaaggtgaag aagctagctt tattgtgatg
     7681 gcagatggcc aacatgcatt accgctagcg acaagccaag atcataagc
//
LOCUS       DAOQKQ010000125         7707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_4630_length_7707_cov_5.828411, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000125 DAOQKQ010000000
VERSION     DAOQKQ010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 7707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7707
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4630_length_7707_cov_5.828411"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="PKZ49_08850"
     CDS             complement(<1..1075)
                     /locus_tag="PKZ49_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HND36709.1"
                     /translation="MNDKLNIAQANQKALALLLSMANRHGLIAGATGTGKTVTLQTLS
                     EQFSQAGVSVFMADIKGDLSGLSQKGGGNSKVEDRIKELGLNQFNYRDNPVVFWDVFG
                     KKGHPMRATISDMGPLLLARMLNLNETQTGVLTTAFKVADDNGWLLLDLKDLRAILQY
                     AAENSQQLVTTYGNISAASVGAIQRALLQLEHEGGESLFGEPMLNFADLMQTDQHGHG
                     VINILSAEKLHNSPRVYATLLLWLLSELYENLPEVGDVEKPKLVFFFDEAHLLFNDAP
                     KVLLEKIEQVVRLIRSKGVGVYFVSQNPLDIPDMVLGQLGNRVQHALRAFTPRDQKAV
                     KSAAQTFRVNPDIDIETVITELGV"
     gene            1302..2804
                     /locus_tag="PKZ49_08855"
     CDS             1302..2804
                     /locus_tag="PKZ49_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HND36710.1"
                     /translation="MVIPFDPVILWTDAFIYLLIGIVVAISGYIRRHPHLVAPWRRVT
                     HSASGMSAFTILVFFVSIGILDTIHFRPAVESSSHETNTQIFSVEVLSLLDVIATPLH
                     TNVEKTYSAPLSAYLYAKETIELPDGGQVREFARLTFGGAHLVNPETDHLQDITIRSI
                     KGAGWGLFAWSIIIFSITIWLAYQNKTRYWQTQQALWRGQTETPWWAIFITLNVIIVL
                     SGAVIALATHYHVLGTDKVGQDVLYLSLKSIRTGLVIGTLTTLIMLPFALFLGIAAGY
                     FRGWVDDVIQYTYTTLSSIPSVLLIAAAVLMMQVYIETHPDLFETILSRADLRLLFLC
                     IILGITSWTSLCRLLRGETLKLREMEYIQAAHAFAVSHWRIISRHILPNVFHILLIAI
                     VMDFSGLVLAEAVLSYVGVGVDPSMISFGTMINAARLEMAREPMVWWTLLAAFGFMFT
                     LVLSANLFADAVQNAFNPRIRTLPLKTIQPAKLTATQNTISVKQKNIPSI"
     gene            2801..4864
                     /locus_tag="PKZ49_08860"
     CDS             2801..4864
                     /locus_tag="PKZ49_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HND36711.1"
                     /translation="MNPLLEINQLSTVLYTGGNPIRAVDGLSLTIYKGETFALLGESG
                     CGKSMTALSIMRLLPEAGQIVEGQIKLADHDLLQLPEAEMRNIRGKRISMIFQEPMLS
                     LNPVMTISAQIDEVLQHHFDLPNQIKQQRIKELLQQVGISDIERRLSEYPFQFSGGMK
                     QRVMIAMALAGQPQLLIADEPTTALDVTIQAQVLNLMKAIQKNTQMSILLITHDLGVV
                     AKMADRIAVMYAGEIIETARREAFFSNPLHPYSQKLFAALPDKNKRQHGLAVIRGNVP
                     PLSQTFSGCRFVDRCDQAVKQCYEHVPSWTDTNTDHYVKCHLFNQPQQLVSRQTENAI
                     PIARSPSNSPNHELLHVTDLKVHFPIYSGLLKRVSGHVKAVDGISLAISSGKTLALVG
                     ESGCGKTTVGKSILQLIPVTAGSIQFNGIELTKLSPKALRNGRSDYQIIFQDPYSSLN
                     PKMRIFEILKEGIDALGIDKHDTVHRSELQTKLSSSSIETRIDELLQQVNLPAEAKWR
                     YPHEFSGGQRQRIAIARALSVNPKLLVCDEPTSALDVSVQAQILNLLSKLQHDFGLAF
                     LFITHNISVVEYLADEVAVMYLGRIVEKGTVDEVLNHAKHPYTQALLSAVPTINPQPD
                     HAQIELSGELPSLSHTPVGCYFHPRCPRAMPICQQSYPEATVFTATHAAHCHLYEHAV
                     TKAVI"
     gene            4894..6495
                     /locus_tag="PKZ49_08865"
     CDS             4894..6495
                     /locus_tag="PKZ49_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HND36712.1"
                     /translation="MLEQEVKRRRTFAIISHPDAGKTTLTEKLLMFAGAIHIAGSVKA
                     RKASRYATSDWMEIEKQRGISVASSVMQIEYNDHVINLLDTPGHQDFSEDTYRVLTAV
                     DAALMVIDAANGVESQTLRLLEVCRARNTPILTFINKMDREVRNPLDLIDEIERTLGM
                     DAIPFTWPIGMGKQFRGVFDLQNRCVRVFQPGADRIDAEPELINNFGDDQIATRFGNE
                     FLEAKQEIDLISGASPQFDQSSFLAGEQTPVFFGSAINNFGVREVLDALINLAPPPGP
                     REALQRIVSPQEKKFSGVVFKIQANMNPAHRDRIAFIRICSGEFKRGMNLKVTRNNKE
                     IRTNTVVSFLSQRREILETAYPGDIIGIPNHGTLQLGDTLTEGEILQFTGLPFFAPEI
                     FQTVEINDPMRTKQLKLGLTQLGEEGAIQVFRPHLGSTLLLGAVGHLQFEVVTHRLQH
                     EYGVDARIASAKYQLARWITSDNPKDLQRFIDLNSHRIAYDAVDAPTFLASFEAELSV
                     AKENWPSIHFHKMREHAGLVFQSHA"
     gene            6608..7054
                     /locus_tag="PKZ49_08870"
     CDS             6608..7054
                     /locus_tag="PKZ49_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36713.1"
                     /translation="MTKTPIVLLFFSILTLFTLANSKLADAHRTIIHNRSFSYTPWSY
                     GLGYTTGLYGYRSYYGYYLNPSYGNFPLIYPYPDPFLYPRYRAPIYYSPLINLPLTPP
                     VYIQQSESPPLRENNYWYYCEIPAGYYPDVKDCQTEWIKVPPRLAE"
     gene            complement(7105..>7707)
                     /locus_tag="PKZ49_08875"
     CDS             complement(7105..>7707)
                     /locus_tag="PKZ49_08875"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HND36714.1"
                     /translation="KIQATNIPFFIPETESIEFDWAGETARLLGKVLHRLLQHIGKMG
                     IEAFKEDDIHKLQQIGRVLLKQSGIMADRFDWAVEQLSTALNTLWQDEKAKWILSNRH
                     HDIHSEWALTGFRQGELDHIVIDRSFIDAQAVRWIIDFKTGTHRGTDIDDFLGQELQR
                     YRPQLERYAQLLQSMETRTIRLGLYFPILGKWLEWGFKDE"
BASE COUNT         2167 a         1796 c         1534 g         2210 t
ORIGIN      
        1 ctacaccaag ttcggttatt actgtttcaa tatcaatatc tggattaact cggaatgttt
       61 gtgctgctga cttaaccgct ttttgatcac gtggcgtaaa cgctcttaat gcatgttgta
      121 cacgattccc taattgaccg agaaccatat cgggaatatc caatggattt tgactgacaa
      181 agtatacccc aacgccttta gagcgaatta aacgaacaac ttgctctatt ttctccaaaa
      241 gtactttagg ggcatcatta aaaagtaaat gggcctcatc gaaaaagaag actaatttag
      301 gcttctcaac atcccctact tccggtaaat tttcatatag ttcagataat aaccaaagta
      361 ataaggttgc ataaactcgt ggcgaattat gcagcttctc agcggataag atattgatca
      421 cgccatggcc atgctgatcg gtttgcatta ggtctgcaaa attcagcatg ggttcgccaa
      481 ataatgattc ccctccttca tgttctagtt gcagcaaagc acgctgaatt gcgccaacac
      541 ttgctgcaga aatattgcca taggtggtta caagctgttg tgagttctcc gcagcatatt
      601 ggagtattgc acgcaaatct ttcaaatcga gcaataacca accattgtcg tcggcaactt
      661 tgaaggccgt ggtcaaaact ccggtctgag tttcattgag attcaacatg cgagccaata
      721 ataacggccc catatcggaa atcgtggcgc gcatcggatg accttttttt ccaaaaacat
      781 cccaaaaaac aacagggtta tccctataat taaactggtt aagccctagc tctttgattc
      841 ggtcttctac tttgctgttt cctcccccct tctgactcaa tcctgacaga tcccctttga
      901 tatctgccat aaatacagac acacccgcct gcgaaaactg ttctgacaac gtctggaggg
      961 tgactgtttt accagttcca gttgcgccgg caatcaatcc atggcgatta gccatcgaca
     1021 acaacaacgc caacgctttc tgattagcct gtgcgatatt gagcttatcg ttcataattc
     1081 atccctatgc aagtagaact taacgctaaa agtatcttga ttgataaatc ttatcattcg
     1141 aaatcagcct tgcctatgtg attagaggaa agctttgtta ttgtcgaaaa ctgtggaaaa
     1201 ccgttgttgt cgatcacttt cacgctccat caatatttcc actacaatat cgcattattc
     1261 tcacactatc gacaccccaa tttactgcaa ctaagcgcta aatggttata ccgtttgatc
     1321 ctgttatttt atggactgat gcattcattt atttactaat tggaattgtt gtcgccatta
     1381 gcggttatat ccggcgccat ccacacttag tagcaccttg gcgcagagtc acgcatagcg
     1441 ccagtggaat gtccgcattt accattcttg tattttttgt gtccattggg attttagata
     1501 cgatccactt ccgccctgct gtcgaatctt cttcccatga aacaaacact caaatattta
     1561 gtgtcgaggt actaagtttg ctcgatgtga ttgcaacgcc actgcacact aatgtcgaaa
     1621 aaacctattc tgcaccgcta tctgcctatc tctacgccaa agaaacgatt gagctacccg
     1681 atggcgggca ggtcagggaa tttgctagat taacctttgg gggtgcccat ttagtaaacc
     1741 ctgaaaccga tcaccttcaa gacatcacta tacgcagtat taaaggtgca ggatggggtt
     1801 tattcgcttg gagcatcata atcttcagca ttaccatttg gctcgcatat cagaataaaa
     1861 ctcgctattg gcaaacccag caagcactat ggcgtggcca aaccgagaca ccatggtggg
     1921 ctatttttat cacgctcaat gtcattatcg tgttatcggg cgctgttatt gcccttgcaa
     1981 cccattacca tgtgctgggc acggataaag tcggtcaaga cgtgctctat ctatcactga
     2041 aaagtatacg aacagggcta gtcatcggta cactgactac attgatcatg ctaccatttg
     2101 ccttgtttct cggcatcgca gcagggtatt tccggggctg ggttgatgat gtgattcaat
     2161 atacctacac aacgctgagt tcgattccaa gtgtactctt gattgcggct gcagtcctga
     2221 tgatgcaggt ttatatcgaa acgcacccag acttattcga aactattcta tctcgcgctg
     2281 atctgcgctt actatttctg tgcatcatct taggtattac tagctggacc agtctatgtc
     2341 ggctactacg cggcgaaacg ctaaagttaa gggaaatgga gtatattcaa gcggcgcatg
     2401 cctttgcagt ttctcactgg cggatcatta gtcgccatat ccttcccaat gtatttcaca
     2461 tcctcctaat cgccatcgtt atggatttta gtggcttagt cctcgcagaa gccgtgttat
     2521 cgtatgtcgg cgtgggagtt gatccatcca tgatcagctt tggcacgatg atcaatgcag
     2581 cacgacttga aatggcgcga gaacctatgg tttggtggac attactggca gcatttggat
     2641 tcatgtttac attggttttg agtgcaaatc tttttgctga tgcagtacaa aacgctttca
     2701 atcctcgtat taggacatta ccacttaaaa ccatacaacc tgctaagtta actgcaactc
     2761 aaaacacaat ttcagttaag caaaaaaaca ttccttcaat atgaatccgt tactggaaat
     2821 taaccagctt tcaaccgtcc tttatacggg tggcaaccct atacgggcgg ttgatggact
     2881 ttctctgacg atatataagg gtgaaacctt tgcgttgctt ggagaatctg gctgtggcaa
     2941 atcaatgaca gcactttcta tcatgcgatt attgcctgaa gcagggcaaa tcgttgaagg
     3001 tcaaatcaag ctagctgatc acgatctcct ccaattacca gaagctgaga tgcgaaatat
     3061 ccgtggaaaa cggattagca tgatatttca ggaaccaatg ctgagtttaa atccggttat
     3121 gacgatttca gctcaaatcg atgaagtgct ccaacatcat ttcgatttac ccaatcagat
     3181 caaacagcaa cgaataaaag agttgttgca acaggttggt atttccgata tcgagcgccg
     3241 cctatctgaa tatccattcc agttctctgg tggaatgaaa caacgtgtga tgattgccat
     3301 ggcattagcg ggacaaccac aattattaat cgccgacgaa cccacaacag cactcgatgt
     3361 taccattcag gcgcaagttc tgaatttgat gaaagccata caaaaaaata cgcagatgtc
     3421 gattttgctc attacccatg atctcggtgt agtagcaaaa atggctgatc gcattgctgt
     3481 catgtatgca ggtgaaatca tcgaaaccgc acgacgtgaa gctttctttt ctaatccact
     3541 gcatccgtat tcacaaaaac tatttgcggc attaccagat aaaaacaaac gccagcatgg
     3601 tcttgctgtg attcgcggta atgtaccgcc tttatcacaa actttctcag gatgccgctt
     3661 tgttgatcgc tgtgatcagg ctgtaaaaca gtgctatgag catgtgccaa gctggaccga
     3721 cacaaacacc gatcattacg tcaaatgcca ccttttcaat caacctcagc aactggtttc
     3781 gaggcaaact gaaaatgcaa ttccaatcgc tcgatcacct tccaactccc ccaatcatga
     3841 attactccac gttacggatt taaaagttca tttcccgatt tacagtggcc ttttgaaacg
     3901 agtatcaggc catgtcaagg ctgtcgatgg tatttcatta gcgatatcgt ctggaaaaac
     3961 gcttgcccta gtgggtgaat cgggttgtgg taaaacaacg gtgggtaaaa gtattttaca
     4021 gcttatccct gtgacggcag gaagtattca attcaatggt attgaactga ccaaactatc
     4081 gcctaaagca ttacgcaatg gaagatccga ttatcaaatt atttttcaag acccttattc
     4141 ttcccttaat cccaagatgc gcattttcga gattctaaaa gaagggatcg atgcgttagg
     4201 aattgataaa cacgataccg ttcatcgttc ggaattacag actaagctat cctcaagttc
     4261 gattgaaacg cgaattgatg agctccttca acaagtcaat ttacctgctg aagcaaaatg
     4321 gcgataccca catgaattct cgggggggca acgccagcgt atcgcaattg caagggcatt
     4381 atcggttaac ccaaaattat tggtctgtga tgaacccacg agcgcacttg atgtttcagt
     4441 tcaagctcaa atattaaatt tattgagtaa actacagcat gattttggtc tcgcctttct
     4501 ttttattaca cataatatct ctgtcgttga gtatcttgca gatgaggtag ctgttatgta
     4561 tctgggaaga attgtggaaa aaggaaccgt cgatgaagtg ttaaatcacg ctaagcaccc
     4621 gtatactcag gcactattat ctgcagtacc aaccatcaat ccacaaccag accatgcaca
     4681 aatagaacta agtggtgaac ttccttcgtt gagccatacc cccgttggct gttatttcca
     4741 ccctcgttgc ccgagagcca tgccaatttg tcagcaatcc tatcccgaag cgacggtatt
     4801 tacagcaact cacgcagctc actgtcatct ctatgaacac gccgtaacaa aagcggtcat
     4861 ataacaactc aaataatttt aggacttacc gcaatgcttg aacaggaagt aaaaagacgc
     4921 cgcacctttg caattatctc tcacccggat gcaggtaaaa ccacgttgac tgaaaaactt
     4981 ttgatgtttg cgggtgccat ccatatcgct ggtagtgtta aagcacgtaa ggcaagccgc
     5041 tacgccactt cagattggat ggaaatcgag aaacaacggg gaatttcggt agccagttcc
     5101 gtcatgcaga tcgagtataa cgatcacgta atcaatttat tagatacgcc aggacaccag
     5161 gatttttcgg aggatactta tcgagtactc accgctgttg atgctgcctt gatggtaatc
     5221 gatgccgcca atggtgtaga gtcacaaact ttacggcttc tggaagtatg tcgggcacgc
     5281 aatacaccga ttctcacttt tatcaataaa atggatcgag aagttcgaaa cccgctcgac
     5341 ctgatcgatg aaatcgaacg aaccttaggt atggatgcga ttcccttcac ctggccaatc
     5401 ggtatgggaa aacagttccg tggtgttttt gatctccaga atcgatgtgt aagagtattt
     5461 caacccggag cagatcgaat cgacgcggag cccgaattaa tcaacaactt tggtgatgat
     5521 caaattgcga ctcgttttgg aaatgaattc ctagaggcaa aacaagaaat cgatctgatt
     5581 tccggggcat ccccccaatt tgatcagagc agctttctag caggtgaaca aactcccgtc
     5641 ttttttggtt ccgcaattaa taattttggt gtccgtgaag tattagatgc gctcattaat
     5701 cttgctcctc caccaggtcc gcgcgaagcg ttacaacgca tagtgtcacc ccaagaaaag
     5761 aaattttctg gtgtcgtatt taaaattcaa gccaatatga accctgccca tcgtgatcgg
     5821 attgctttta ttcgaatttg ttcgggtgaa ttcaaacgag gaatgaatct taaagttaca
     5881 cgaaataata aagaaattcg aaccaatacg gttgtttctt tcttatcaca acgccgagaa
     5941 attttagaga cagcttaccc gggtgatatt atcggtatcc ccaatcatgg aacccttcaa
     6001 ctgggagata ccttaactga aggagaaata ctgcaattta ctggtcttcc ttttttcgcg
     6061 ccggaaatat ttcaaaccgt ggagattaat gacccaatgc gtaccaagca acttaaatta
     6121 ggattaactc aactcggtga agaaggtgct atccaagtat tccgccctca cctgggtagc
     6181 acgttactgc tcggtgcagt cggtcatttg caatttgaag tcgtcactca tcgcttacag
     6241 catgaatatg gtgtcgatgc acgtattgca tctgctaaat accaattagc acgctggatt
     6301 acttctgata atccaaaaga tctacagcgc tttatcgatt tgaattcaca ccgcattgcc
     6361 tatgatgccg ttgatgctcc aacttttctc gcttcatttg aagcggaatt aagtgttgca
     6421 aaagaaaact ggccttccat tcattttcat aaaatgcgag aacatgcagg gctggttttc
     6481 caatcccacg cttgaaatta aggcagtaag ttaatagccc ttcaattatc actgcaacta
     6541 atgatgtact atttaatcta tagtaaatga tagcaccacg cctcgttagc ttacctcaaa
     6601 aaaacaaatg accaaaacac cgattgtttt gttatttttt tctattctca ccttgttcac
     6661 gctagctaac agtaaattag ccgacgctca tcgtaccatc attcataatc gttcattttc
     6721 ctacacacct tggagctatg gtttaggcta caccaccggt ctttacggat atcgtagcta
     6781 ttatggatat tatctcaacc cttcttacgg gaactttcca ttaatttatc cgtatcccga
     6841 tccttttctt tatcctcgtt accgtgcacc gatttattat tcacctttaa ttaatctacc
     6901 gcttacccca cctgtttata tccaacagtc agagtcaccc cccttgcggg aaaataacta
     6961 ttggtattat tgcgaaatac cagcaggcta ctaccctgat gtcaaagact gccaaacaga
     7021 atggattaaa gttccacctc gattagctga atgaactgta ttgcgaaaaa tttaaagcgc
     7081 tagtaaatct accattgata acagttactc atctttaaac ccccattcca accatttccc
     7141 caaaattggg aaataaagcc ccaatcggat agtgcgtgtt tccatggatt gtagcaattg
     7201 cgcatagcgt tccagttgtg gtcgatagcg ttgaagttct tggcctagaa aatcatcgat
     7261 atcagtcccc cggtgtgttc ccgttttgaa atcgatgatc cagcggaccg cctgcgcgtc
     7321 gataaaactg cgatcgataa cgatatgatc gagttcgcct tggcgaaaac cagtgagcgc
     7381 ccattctgag tgaatatcgt gatggcggtt actcagaatc cacttcgctt tttcatcttg
     7441 ccataaagta ttgagcgcgg ttgataattg ttccactgcc caatcgaatc ggtctgccat
     7501 gatgccactt tgcttcaata gcacacgacc gatttgttgc aatttatgaa tatcatcttc
     7561 cttaaacgcc tcaatgccca ttttgccaat atgttgtaat aggcgatgca atactttacc
     7621 gagcagacgt gcggtttctc ccgcccaatc aaattcaatc gattctgttt cgggaatgaa
     7681 aaatggaata ttcgtggctt gtatttt
//
LOCUS       DAOQKQ010000126         7671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_4663_length_7671_cov_4.731618, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000126 DAOQKQ010000000
VERSION     DAOQKQ010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 7671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7671
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4663_length_7671_cov_4.731618"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1528
                     /locus_tag="PKZ49_08880"
     CDS             <1..1528
                     /locus_tag="PKZ49_08880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HND36715.1"
                     /translation="SVLAIFKAKTDSYIFEFTLPAESKLPVNHPVTLFFFIGNPVYDP
                     ALPKENSDSIQPLIWEYSSSEDRTEWKSLLVEDETQSLTRTGSVEFLVPSDFSKPEGK
                     QTFPIRVRLKNDAPYNPPPSLQWVALNTVNAEQVISVREEILGSGNDNPNQTFSTFRK
                     PVLVGQQLQVLERAAAANSLTNEQSPQDNWVDWKEVTDFYQSSGKDRHYMLDRITGKV
                     RFGDGNNGMIPPPGVRNIRLQYYRSGGGSNGNVPAGSIKSLVTSINKIEKIANFIAAS
                     GGADKETYESLLDRAPKILRHRDRIVTVEDYEDHAKLASPEVARALCVPLIDLAVEPS
                     KVITSADDEENGRGKVSVIIVPHTSGTKPLPSQILLSHVENKLREKSLPGITLSVVGP
                     LYLSTKITLSLQLESLRFEDQIKHELLNKLKAYLHPLTGRNGLGWPFGRKPHESDIYK
                     LIGEVPRINYVSGLQIVFKADKPSDCRSHNPVECIEKSERFLICSDDEHVILIEGIGN
                     "
     gene            1596..2336
                     /locus_tag="PKZ49_08885"
     CDS             1596..2336
                     /locus_tag="PKZ49_08885"
                     /inference="COORDINATES: protein motif:HMM:NF021220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HND36716.1"
                     /translation="MKDKIDNQLLQYLPEIFQQDTTRDFLLAFEKILLGRQDDEATIK
                     EIDKDPQLANQQGLEQILDHLARYFVPGTNPSDSTTPDEFLPWLSQWVALSLRWDITR
                     DANTNNDIRRRFIAEMVQLYRYRGTKKSMQKLLAIFSQADEKDVHIDDEIDDKPYFFK
                     ILLPLEELKDSDHEKEFERRMELAHSVIRLEKPAHTYYQLIPNIITFRIGQRQEPPPT
                     PSGVQLPTAYKVIVGKNTRLGFARRKKE"
     gene            2355..4541
                     /locus_tag="PKZ49_08890"
     CDS             2355..4541
                     /locus_tag="PKZ49_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36717.1"
                     /translation="MTDNTKRNNPRRNQKTSKETLQSAVAQPSTLVAARGSSKGEIER
                     LNYFTGQFLEATDFKDEQEYHIRMRRFGNRALYSAGILDSGFEVQKISDSEISISSGI
                     GIDAQGRELIIITPLKVGVPIDKKSMTLNITLEYVEKEAETSSDTFVDTEGISVTGRT
                     RIAETPAVEFPEDANITNNQIRIAKITLDANGKIGSIDKAERQFAHARFLGVVGIGQK
                     KAPEDWYRQRAGLEINKGDTNEVALLLSSSGLGWGSGIQFKNTAANGKTYGIYSDSIT
                     GSLHITDEDLKTDRLVIDKNGNVGLGTHNPVAKLQVTGGAIMPAAGNDDASGILFPEN
                     PGTGSGDKAWIRYYARDGEKTALEIGVADNSLGTLQDDILLIPSGGVGIGTREPKAML
                     DVEGGAFLGYESTQSNFNAPLKSGFYQGHGNPQGPEPDTQGDVPDTSHDWSHLIVSRH
                     SNTTNNHQFQIASTYLSNDRIFFRKIADNSPNWNEIATRGVNNFLGNQFIRGSLSVEG
                     EVYIRDHLYINGKIAISSANSANSLVLNFGNIFGNGVSCSGLFEPSSLNVGSYMTSTG
                     KLNPGIGNAYFKGKVMAVELSTTFSDLAENFFSDTMLDPGDVVCFDPIEDRIVKSENP
                     ESTMVCGIISTAPGVLLNSDHGTEKEGNFPLALSGRVPCKVTDENGPIKRGDLLCTSS
                     KSGHAMKAQPVVMSDVALYRPSTILGKALGSLESGSGIIDVLVFLS"
     gene            4608..4925
                     /locus_tag="PKZ49_08895"
     CDS             4608..4925
                     /locus_tag="PKZ49_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36718.1"
                     /translation="MTFPIRIDVHSLAYANFKISAVTPDWVDIRAVRVWELAEGAHRL
                     LFDSSAVDFTVTAAGKIEYDPALETYVRGFGTPGLEVTGYPVTVDATALADATVLFRL
                     KTA"
     gene            4939..>7671
                     /locus_tag="PKZ49_08900"
     CDS             4939..>7671
                     /locus_tag="PKZ49_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36719.1"
                     /translation="MAFSIEIDATQLSAIFFNIPGITPAQQPTQQNDLEKRIWPMEPG
                     AYKIYVVGQAYDFTLTEQGVIGYDEKLDISNGGYFAGLGTARLQLLGYPVEIIAEQLS
                     SPRIIYPDITPDWVSTKILQSFRLLPGTYGFRPSDSWASVVYQVTQDGKIGYDADLDV
                     NNDGCFAGLGTTRLQLLGYSVEIIAESLSSPRIIYTDVTPDWVSTQIPQLFQLLPGIY
                     RFQPSDSPALVVYQVTPDGKIGYDADLDANNGGCFTGLGTTRLELLGYPIKITANQLS
                     SPEIIYHNAQPTGEWRKTKDPQAFRLLPGLYNLQVAANYANVSYRVTPQGKIDYEDTL
                     DIKNGGCFSGKESTQLELLGYSIEIIAEKLSTEQIAYPYIIPSLGSKSLQTFRLLPGQ
                     YNLQVAAGYATVIYLVTPQGKIDYDEDLDANNGGCFAGLGTTRLELLGYSVEIVADEK
                     LSTPQITYPYIISGWVSGKTKQIFRLLPGLYNLQTPASFVSVVYTVTPQGKIGYEEPL
                     DVSNGGCFAGKETTQLKLIGFSIAVDSSPLFQPQFLISEATPSDEVAIQKKVTFHLLP
                     GAYTIQSISGSGYIFSVTPKGTIDYDETFDVNNPEAPGFFSGSETTTLTLEGYPITLD
                     ATPLADAISQFGLGNGTRWLTTRESHTVRLFPSRYGLDLVSGAINGAEFEVIFGGTID
                     YDSSLDIQRASGDLRPPGYLSGNNTNKLRLEGYAITLDASPLAQIAGSFRLGSGDATS
                     RLPTNKASKVRLLPTHYSLYFDTGAIAEHLFEVLYGGVIRYATQFDWSNGGFLEGNGE
                     PRLKFLGYTLLIDASELSGTKVVLLPFQYSIGSVSKGIEAIQVMPQANLTLELFTISP
                     QSVVFDMELNGTVTLRKSFPFVILEISSGYPLLKLKKHSLQLEQNNKRAMKINLP"
BASE COUNT         2176 a         1678 c         1796 g         2021 t
ORIGIN      
        1 gtccgtttta gctattttta aagctaaaac ggactcttat atttttgaat ttaccttgcc
       61 tgcggaaagc aagcttccgg ttaatcaccc cgtaacacta ttttttttca tcggtaaccc
      121 ggtttatgat ccagctttac caaaagaaaa ttctgattcg attcaaccgt tgatttggga
      181 atattcttct tcagaagaca gaactgaatg gaagtcactc ctcgttgagg atgaaacgca
      241 gtcattgaca cggaccggta gcgttgaatt tctagtaccc agtgattttt ctaaaccgga
      301 gggcaaacaa acctttccaa tccgtgttcg tttaaagaat gacgctcctt ataatccacc
      361 gccttcattg caatgggtgg cgctgaatac agtcaacgcc gagcaagtaa tttccgttcg
      421 tgaagaaatt ctcggttccg gtaacgacaa tcccaatcaa actttttcca ccttcagaaa
      481 accggtactt gtcgggcagc agcttcaggt actggaacgc gctgctgccg caaacagcct
      541 aaccaatgag caatctccac aagataactg ggtagactgg aaagaagtta ccgattttta
      601 tcaatcttcc ggaaaggatc gccactatat gttggatcgc attactggga aagtacgatt
      661 tggtgacggt aacaatggaa tgattccgcc gccaggggtt cgaaatattc gcttgcagta
      721 ttatcgttca gggggtggta gcaacggtaa cgtaccggca gggtcgatta aatcgctggt
      781 caccagcatc aacaaaattg aaaaaatcgc taactttatt gccgcatctg gcggcgccga
      841 taaagaaact tatgaatcgt tactcgatcg tgcacccaaa attctgcgcc atcgtgaccg
      901 aatcgtgaca gtcgaagact acgaagatca cgccaaactg gcatctcccg aagtggcgcg
      961 cgcactgtgt gtgccgctga tcgatttggc cgttgagcct agcaaagtca ttaccagtgc
     1021 agatgatgaa gaaaacggtc gtggcaaagt cagcgtcatt attgtgccgc atacatctgg
     1081 aaccaaacca ctacctagtc aaatattgtt atcgcacgtg gagaataaac tacgagagaa
     1141 aagccttcca ggtattacac tatcagttgt tggtccgctt tatctaagca caaaaattac
     1201 actttcgctc cagttggagt cattacgttt tgaagatcaa atcaaacatg aattactcaa
     1261 taaattgaag gcttacttgc acccccttac cggtcgtaac ggtctaggct ggccatttgg
     1321 ccgcaagcct cacgaatcag atatctataa acttattgga gaagtaccca gaataaatta
     1381 tgtgagcggt ttgcaaattg tttttaaagc agataagcca tcagactgtc gcagtcataa
     1441 tcctgtagaa tgcattgaga aatcagagcg atttctgatt tgctctgatg acgaacatgt
     1501 aattttaatc gaaggaatag gaaactagat taatgctgga ttatcgctaa ttttctatct
     1561 gaacagcaga agcgcctact aggttagtca acatcatgaa agacaaaatc gataatcagc
     1621 tactgcaata tttaccagag atttttcaac aagacactac aagggatttt ttgttggctt
     1681 ttgaaaaaat cctgttgggg cgccaggatg atgaagcaac catcaaagaa attgataaag
     1741 acccacaact tgcaaatcaa caggggttgg agcaaattct tgatcacctt gctcgttact
     1801 ttgtgccagg taccaatcca agtgacagca ccaccccgga tgaattcttg ccatggctca
     1861 gtcaatgggt agcactatca ctgcgctggg atatcacccg tgacgctaat actaataacg
     1921 acatccgccg tcgtttcatt gccgaaatgg tgcagctcta tcgttatcgt ggtaccaaga
     1981 agagtatgca aaagctcctc gcgatatttt ctcaagccga tgaaaaagac gtgcatatcg
     2041 atgacgaaat agatgataaa ccctattttt tcaagatact gctgccgctt gaagaactca
     2101 aagacagtga tcatgaaaaa gaattcgagc gacgcatgga gctagcccac tcggtgatcc
     2161 gcctggaaaa accggcacat acatactacc agctgattcc aaatattata actttccgca
     2221 taggtcagcg ccaggaacct cctccaacgc caagtggcgt ccaactgcca acagcataca
     2281 aagtgatcgt gggcaaaaac acccggctcg gatttgcccg ccgcaaaaaa gaatagataa
     2341 ggagatgagt tatcatgacc gacaacacta aaagaaataa tccccgtcgt aaccagaaaa
     2401 ccagcaaaga aacccttcaa agcgctgttg cgcaaccctc aacactcgtt gctgcgcggg
     2461 gatcaagcaa aggagagatc gaacggctga attattttac cggccaattc ctggaagcca
     2521 ccgatttcaa ggatgagcag gagtaccaca ttcgcatgcg ccggttcggt aatcgcgcgc
     2581 tatacagcgc cggtattttg gatagtggtt ttgaggttca aaaaataagc gattcagaaa
     2641 tctctatttc atcgggtatc ggtattgatg cacaaggccg tgaactgatt attatcactc
     2701 cgttgaaagt cggagttcca atcgataaaa aatcgatgac actgaatata acgctcgagt
     2761 acgttgaaaa agaagctgaa acaagctccg atacttttgt ggacactgag gggataagcg
     2821 tcaccggaag aacccgcatt gctgaaacac cggcagttga gtttcccgag gacgcgaata
     2881 tcacaaataa tcagataaga attgccaaaa ttactctcga tgcgaacggt aagattggta
     2941 gtatcgataa agctgaacgt caatttgcgc atgcacggtt tttgggggta gttggcatag
     3001 gacaaaagaa agcacccgaa gattggtacc gtcagagagc tggattggag atcaacaaag
     3061 gtgacacaaa tgaagttgct ttactcttga gttcctctgg acttggctgg ggatcgggta
     3121 ttcaattcaa gaatactgca gcaaatggca aaacctatgg aatttactct gactcaatca
     3181 cgggaagttt gcatatcacc gatgaagatc tcaaaactga tcggcttgtt atcgataaaa
     3241 atggtaatgt tggtcttggc acacataatc cagttgcaaa gctacaggtt actggtggag
     3301 ctattatgcc agcagcaggg aatgatgatg cttccggaat attgtttcct gagaatccgg
     3361 gcacagggag tggagataaa gcctggatta gatactacgc ccgagatggt gagaaaactg
     3421 cgttggaaat cggagttgct gataattcgc tcggcacact tcaagatgat attttactga
     3481 taccttctgg aggagtggga attggcactc gtgaacccaa agccatgctg gatgttgagg
     3541 gtggggcttt tttgggttat gagagtacac agtctaattt taatgctcct ttgaaatcag
     3601 gtttttacca aggtcatggt aacccgcagg gacctgagcc agatacgcaa ggagatgtgc
     3661 ccgatacaag tcatgattgg tcacatttga ttgtttctcg tcatagtaat actactaata
     3721 atcatcagtt ccagatcgca tccacctatt taagtaacga ccgaattttc tttcggaaaa
     3781 ttgcggataa ttctcctaat tggaatgaaa tcgcaacgcg aggtgttaat aattttttag
     3841 gaaatcaatt catcagaggg tctttatcag ttgagggtga agtttacata agagatcatc
     3901 tatatataaa cggtaaaatt gctattagct ccgctaattc ggctaatagt ttagtattga
     3961 actttggtaa catttttgga aatggcgtgt cttgttcagg actatttgaa ccaagcagtt
     4021 tgaatgttgg ttcctatatg acgagtacag gcaaattaaa tcctgggatt ggcaatgcat
     4081 actttaaagg gaaggtaatg gcagtggagc tcagtacgac gttttcggac ttggcagaaa
     4141 actttttctc cgacacgatg ctagaccctg gagatgtagt gtgctttgat ccaatcgaag
     4201 atcgcattgt gaaatcggaa aatccagaat caaccatggt atgcggaatc atatcaaccg
     4261 cacctggggt tttattaaat tctgaccatg gtacagaaaa agaaggaaat tttccattgg
     4321 cattgagtgg acgagtacct tgtaaagtca ctgatgaaaa cggaccaata aaacgaggtg
     4381 atttactttg cacatcatcc aaatcgggcc atgcgatgaa agcgcaacca gttgtgatga
     4441 gtgatgtagc tctttatcgc ccgagcacga ttcttggtaa agcccttggt tcattggagt
     4501 caggatcagg aatcatcgat gttctggttt ttcttagtta gtcagacttt aaatgtaaga
     4561 atttcgtaca atcaaccgag aagttaacag cgccgtcatt cataaccatg acttttccca
     4621 tccgaatcga tgttcactcg cttgcatacg ccaatttcaa gatttccgct gtaacgccgg
     4681 attgggtgga tatccgtgcg gtacgagtct gggaacttgc agagggtgcg catcgtttgc
     4741 tgtttgacag cagcgcggtg gattttaccg tcacggcggc gggaaaaatc gaatacgatc
     4801 ccgcgcttga aacctatgta cggggttttg ggactcccgg tttggaggtg acagggtatc
     4861 cagtcacggt ggatgcaacc gcacttgcgg acgctacagt tctattccgc ttaaagactg
     4921 cgtgagcatc gaataactat ggcattctca atcgagatcg atgcaaccca gctttccgca
     4981 atcttcttta acattccagg cataacccct gcacagcaac ctactcagca aaacgatctc
     5041 gagaaacgca tttggccgat ggagccaggt gcttacaaaa tttatgtggt tggacaggca
     5101 tacgatttta ctctaaccga acaaggcgtc atcggttacg atgaaaaact ggatatcagc
     5161 aacggtggct actttgctgg acttggaact gcccggctgc aattgctggg ttatccggtt
     5221 gaaatcatcg ctgagcagct atcgagcccg aggattattt atccagacat taccccggat
     5281 tgggtgagca ctaaaatcct tcaatcattc cggcttttac cgggaacata cggatttcga
     5341 ccctcggata gttgggcatc tgtggtttat caggtaacac aggacggaaa aatcggctac
     5401 gacgcggatt tggatgttaa taacgatggt tgctttgcgg ggctcggaac tactcgattg
     5461 caattgctgg gttattcggt tgaaatcatc gctgagtcgc tatcgagtcc gagaattatt
     5521 tacacggatg ttactccgga ttgggtgagc acccagatcc cgcagttgtt ccagcttttg
     5581 ccagggatat acagatttca accctcggac agtccggcat tagtggttta tcaggtgacg
     5641 ccggacggca aaatcggcta cgacgcggat ttggatgcca acaacggcgg ctgtttcaca
     5701 ggactcggga ccacccggct ggaattgctc ggctatccga tcaagattac tgcaaaccag
     5761 ttgtcgagcc cggagatcat ttatcacaac gcacagccta ccggagaatg gcggaaaacc
     5821 aaggatccgc aagcattccg gcttctgccg ggactatata atctccaggt tgccgccaac
     5881 tatgcgaatg taagttatcg cgttacccct cagggcaaga tcgattacga agatactctg
     5941 gatatcaaga atggaggttg tttttcgggg aaagaaagta ctcagttgga attgctcggt
     6001 tattccatag agatcattgc tgagaaattg tcgaccgagc agatcgctta tccctatatt
     6061 atcccaagtt tgggaagcaa gtctttgcaa acattccggc ttttgccggg acaatacaac
     6121 ctccaggtcg ccgctggcta tgcgacagta atttatcttg ttacccctca gggcaagatc
     6181 gattacgacg aggatttgga tgccaacaac ggcggctgtt ttgcaggact cgggaccacc
     6241 cggttagagt tgctcggcta ttctgttgag atcgtcgctg atgaaaagct gtcgaccccg
     6301 cagatcactt atccttatat catttcaggc tgggtgagtg gcaaaaccaa gcaaatattc
     6361 cgacttttgc ctggattgta caatcttcaa acacctgcga gttttgtatc ggtagtttat
     6421 accgtgacac cgcaaggtaa aataggctac gaagaacctt tggatgtaag caatgggggg
     6481 tgttttgctg gaaaagaaac cacgcaattg aagctgatag gtttctcaat tgcagtagat
     6541 tccagcccac tttttcaacc gcagttcctg atttcagaag caacacccag tgatgaggtg
     6601 gcgatacaga aaaaagttac attccatcta ctccccggcg cgtatacgat ccagtcaatc
     6661 tccggatctg gttacatttt ttcggttact ccaaaaggga ctattgatta tgatgaaact
     6721 ttcgacgtca acaatccgga ggctcctggc tttttttctg gcagcgagac gactacgctg
     6781 acactggagg gttatccgat tacgctggat gcgaccccgc tagctgatgc gattagccaa
     6841 tttggactgg gtaatggaac tcgttggttg actacccgcg aatctcatac tgtgcgcttg
     6901 tttccgtccc gctatggctt ggatcttgtc agtggcgcga taaatggcgc cgaattcgag
     6961 gtgatctttg gaggaacgat tgattacgat tcgagcttgg atattcaacg cgcttcaggc
     7021 gatctgcgcc ctcctggcta tttgagcggt aataacacaa acaaattgcg attggaaggc
     7081 tatgcaatta cattggacgc ttccccttta gcacaaatag ctggctcatt tcgtcttggt
     7141 agtggcgacg ctaccagccg attgcccacc aataaggcaa gtaaagtacg cttattgcct
     7201 acccattaca gcttatactt cgatactgga gcaatagccg agcatttatt tgaggtgctt
     7261 tatggtgggg tgattcgcta tgctacgcag tttgattgga gtaatggcgg tttcttagaa
     7321 gggaatggtg agcctcgatt aaaattcttg ggttacacac tacttattga tgccagcgaa
     7381 cttagtggga caaaggtagt actattacca ttccaataca gtattggttc agtatcaaaa
     7441 ggtatagagg ccattcaagt aatgccacag gccaatttga cattagaact atttacgata
     7501 tcaccgcaat cagttgtgtt tgacatggaa ctgaacggta ctgttacctt gagaaaatct
     7561 tttccattcg tgattcttga gataagtagc ggatacccat tactcaaatt gaaaaaacat
     7621 tctctacaac ttgagcaaaa taacaagcgt gctatgaaaa taaacttacc g
//
LOCUS       DAOQKQ010000127         7624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_4704_length_7624_cov_5.414057, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000127 DAOQKQ010000000
VERSION     DAOQKQ010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 7624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7624
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4704_length_7624_cov_5.414057"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="PKZ49_08905"
     CDS             complement(<1..792)
                     /locus_tag="PKZ49_08905"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HND36720.1"
                     /translation="MELSKRAQAIKPSPTLAVTAKAAKLKSEGKDIIGLGAGEPDFDT
                     PQHIKAAAIAAIQAGFTKYTQVGGTPALKQAIVAKFKRENGIEYTTKQVTVSCGGKQS
                     FFNLVLSTINPGDEVIIPAPYWVSYPDIVLIAEGKPVFIDTGIADNFKITAAQLEAAI
                     TPKTKMFVINSPSNPSGSVYSLEELKALGQVLLKYPNILIATDDMYEHILLTEGKFSN
                     ILNACPELYPRTIVLNGVSKAYAMTGWRIGYCGGPETIITAMENIQ"
     gene            868..2982
                     /gene="uvrB"
                     /locus_tag="PKZ49_08910"
     CDS             868..2982
                     /gene="uvrB"
                     /locus_tag="PKZ49_08910"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HND36721.1"
                     /translation="MIITFPNSPFKLHQPFEPAGDQPEAISKLEAGIHAGLMFQTLLG
                     VTGSGKTFTIANTIARLGRPALIIAPNKTLAAQLYAEMREFFPENAIEYFVSYYDYYQ
                     PEAYVPSRDLFIEKDSSINEHIEQMRLSATKSLLERKDTIIVATVSCIYGIGDPVDYH
                     GMILHLREQEAISQRDIIKRLTEMQYQRNEFEFNRGTFRVRGDIIDVFPAENSELALR
                     VALFDDVVESISAFDPLTGRILQKIARFTVYPSSHYVTPRSTTLKAIETIKTELAERA
                     NYFYQNTKLVEAQRIEERTRFDLEMLNELGFCKGIENYSRHLSGRKPGEPPPTLIDYL
                     PREALLIIDESHVTVPQIGGMFRGDRSRKENLVTYGFRLPSALDNRPLKFEEFEQLMP
                     QTIFISATPADYENQHSGQVVEQVVRPTGLIDPQIIVRPVLSQVDDLMSEVNIRVKQG
                     ERVLVTTLTKRMSEDLTDYFSDHGIRVRYLHSDIDTVERVEIIRDLRLGKFDVLVGIN
                     LLREGLDIPEVSLVAILDADKEGFLRSERSLIQTIGRAARHIHGTAILYADRITNSMR
                     RAIDETERRRQKQLLFNEAHHITPKGVSKRIKDLIDGVYCSETAQNNLRAAQAEARYE
                     NMSESRMAQEIKRLEKQMLATAKNLEFEKAAELRDEIKKLKDKLFVGYVEPEINPSNT
                     NNAQPLAMNPKQGVIRRVKKQK"
     gene            complement(2986..3309)
                     /locus_tag="PKZ49_08915"
     CDS             complement(2986..3309)
                     /locus_tag="PKZ49_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfJ"
                     /protein_id="HND36722.1"
                     /translation="MRRLLVSIIFSLFVFISTQGYAEQPAQPLPQNEGIVISTLDTTG
                     YTYMELGNGSKRFWIAAPTTQVKKGDHIRFVESMSMHNFTSKTLNKTFDRIIFVTSTQ
                     VKLAQ"
     gene            complement(3332..4972)
                     /locus_tag="PKZ49_08920"
     CDS             complement(3332..4972)
                     /locus_tag="PKZ49_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HND36723.1"
                     /translation="MNLFGKKYWINRFLTIATISCTFQSLANEPAKAASANPSNTTNT
                     AAPAAPNAAPEKFNWGGVPPVTPMPRPGLFVIPPAGPGYFSLWDLISGNKREAAPVAP
                     YAPFGLLPTSGFDVDFRYLEKPDHEKDVFDPFKRIKLADNWMLSFGGQFWYRYMHETD
                     SRLNAGGQDNKYHLFRTRFHADLWYRDQFRLFAEFIDARALGLDLPALTTDRNYTDIL
                     NLFADVKLGQLVDGPVYMRVGRQELLYGSQRLISPLEWVNTRRTFQGVKAFWRTPTFD
                     LDAFWVRPMTIERNEFDNWDKGRNFMGLWGTYKPMKGQLLDLYYLSLVDNRILNPFTV
                     QQGNILQGDSVLHTLGGRVAGDYNRFLYEFEGMYQFGRRSHLDISAFAVATGLGYHVP
                     LPMNPQFWIRYDFASGDANSQDGRSNTFNHLFPFGHYYMGFLDQVGRQNIHDFNAQFT
                     LHPQPWFTFIAQYHRFYLANTRDFLYNAAGLATLRDPTGQSGSHVGDEIDFRVNVHVS
                     RHQDVLFGYSKLFAGDFLRAQRPGISPDFFYAQYVIRF"
     gene            5254..6405
                     /locus_tag="PKZ49_08925"
     CDS             5254..6405
                     /locus_tag="PKZ49_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HND36724.1"
                     /translation="MLNESEKNQVSPWYQLNTEGGIHSLKLTGSYTLGALNNEIKKLG
                     TRLEYFANKPNVQWDLTGIETLDHVGLAMIWRSWGKQRPSQVLLRIDQEKLFDHLAQS
                     SQALAKPKWQADLLWPIVFLGKRFFLFWNHLTGIITLLGQVILDLFYLVRHPRQIPLR
                     EISANLYRTGAQALGITAVVGFLIGIVLSYLMSQQLHTFGADVFIVNILGMSIIRELG
                     PMLAAILVAGRSGSSMTAQLGVMRVTEELDAMTVMGIPHSMRLVLPKIIGLGIAMPLV
                     VLWTSASALLGGILAADLQIGLSYQYALMNLPNAVPVANLWIGLGKGLVCGMAIALIA
                     CHYGLRIKPNTESLGEGTTNSVVTSITAVILIDAIFAILFSNVGLRFSR"
     gene            6405..7187
                     /locus_tag="PKZ49_08930"
     CDS             6405..7187
                     /locus_tag="PKZ49_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HND36725.1"
                     /translation="MMESGSIIEINGLYTQFGPHLIHRNINLNVLKGEVLSLVGGSGS
                     GKTTLLRQMLGLETPSQGSVKIFGCSRYACNLDTLKAIRHRSGVLFQQGALFSALTVF
                     DNIALPMRELHCLDEALIHDLVMLKLKMVEVGAEHANKRPAELSGGMIKRVALARALA
                     LNPELLFLDEPTAGLDPSLSESIVDLVRALREELTLTIVMVTHDLDTLAAISTRIAVL
                     ADQHIVALGKLQEVVQVDHPFIKDFFWGERGQNALRKYLIAD"
     gene            7283..>7624
                     /locus_tag="PKZ49_08935"
     CDS             7283..>7624
                     /locus_tag="PKZ49_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HND36726.1"
                     /translation="MSGDTLTLAQMLISRRSLTPRDDGCQKLLENRLKALGFQIEKLT
                     FGDVENLWARRGTKQPLVCFAGHTDVVPTGPLGEWESDPFVPTIRDNRLYGRGAADMK
                     ASLAAFITAIES"
BASE COUNT         2076 a         1569 c         1830 g         2149 t
ORIGIN      
        1 ttggatattt tccatggcgg tgatgatcgt ttcaggcccg ccacaataac caatgcgcca
       61 accggtcatg gcataggctt tggaaacgcc attcagaacg atggttcgtg ggtataactc
      121 agggcaagca ttcaaaatat ttgaaaattt accttccgtt aataagatat gttcgtacat
      181 atcatcggtg gcaatcagta tattagggta tttcagcagt acctgcccta atgctttcag
      241 ttcctcaagg ctatagacac tgccggatgg attgctgggg ctgttgatca cgaacatctt
      301 ggtttttggg gtaatcgctg cttcaagctg tgcggcggta attttaaaat tatcagcaat
      361 tccggtatcg ataaaaaccg gtttgccttc cgcaatgaga acgatgtcag gataggatac
      421 ccagtaaggc gcgggaataa tgacttcgtc ccccgggttg atagtggata gaaccaaatt
      481 aaaaaaactt tgtttcccac cgcaggacac ggtgacttgt tttgtggtgt attctattcc
      541 attttctcgc ttgaattttg cgacaatcgc ttgtttgagt gccggtgttc ccccaacttg
      601 ggtatacttg gtgaatccgg cttgaatggc tgcaatcgca gcggctttaa tatgttgcgg
      661 cgtatcaaaa tcaggttccc cagcgcccag tcctataata tctttacctt ctgacttgag
      721 tttagccgcc ttggctgtga ctgcaagggt aggggaaggt ttgattgctt gggcccgttt
      781 tgaaagttcc actgctgtgc tcctgattga gttgatgctg ccgtgttaaa atttagaaat
      841 tagtttgcga ttcacgaaat tatacctgtg attattacct ttcccaacag tccatttaag
      901 ctacatcaac catttgaacc tgcgggcgat cagccggagg cgatcagcaa attagaagcg
      961 ggaatccatg cggggctgat gtttcaaacg ttgctggggg tcactgggtc aggcaaaacc
     1021 tttactattg ccaataccat tgcgcgttta gggaggcccg cgctcatcat cgcgccgaac
     1081 aaaacactgg ctgcgcaact gtacgctgaa atgcgtgaat tttttccaga aaatgccatc
     1141 gagtatttcg tttcttatta tgattattat caaccggaag cttatgtacc ttctcgtgat
     1201 ttgttcatcg agaaagattc cagcatcaat gaacacatcg agcagatgcg gctttccgca
     1261 acgaaatcct tattagagcg taaagatact attattgtgg caacagtttc atgcatttac
     1321 gggattggcg atcctgtcga ctatcatggc atgattctcc atttgcgcga acaagaagcg
     1381 atctcgcaac gcgatatcat taagcgcttg acggaaatgc aataccagcg caatgagttt
     1441 gaattcaatc gcggcacgtt tagagtgcgt ggcgatatca tcgatgtttt ccccgcagaa
     1501 aattctgaac ttgcacttcg tgttgcgttg tttgatgatg tggtggaaag tatttcagct
     1561 tttgatcccc tgacagggcg tatcttgcag aagattgcgc gctttacggt gtatccttcc
     1621 agtcattatg tgacgccgcg gagtaccact ttgaaagcga tcgaaaccat aaaaactgaa
     1681 ttagcggaac gcgctaatta cttttatcag aacaccaagt tagtggaagc acagcgcatt
     1741 gaggagcgaa cccgctttga tctcgaaatg ttgaatgaac tcgggttttg taaagggatc
     1801 gagaattatt ctcgccattt gtcggggaga aagccgggag aaccaccgcc aaccttaatc
     1861 gattatctgc ctcgtgaggc gcttttgatt atcgatgaaa gtcatgtgac agtgccgcaa
     1921 attggtggca tgtttcgagg cgatcgctcc cgcaaagaaa atctggtgac gtatggtttt
     1981 cgattgcctt ctgcgcttga taatcgtccg cttaaatttg aagagtttga acagctaatg
     2041 ccgcagacga tttttatttc tgcaacgccc gctgattacg aaaatcaaca tagtggtcag
     2101 gtggtggaac aagtggtgcg gccaacgggc ttgatcgatc cacagattat cgtacgaccg
     2161 gtactgagcc aggtcgatga tctgatgtcg gaagtgaata tccgagtgaa gcaaggggaa
     2221 cgtgttctcg tcacaacgct aactaagcgt atgtcagaag atttaaccga ttatttctcc
     2281 gatcatggca tcagagtgcg ttatcttcat tctgatattg atacggttga gcgcgtcgag
     2341 atcattcgcg atctaagatt aggaaaattt gatgtgctcg tgggtattaa cttattgcgg
     2401 gaaggattgg atattcccga agtgtcgctg gtggctattc ttgatgcaga taaagaagga
     2461 tttttacgtt ctgagcgttc tttgattcaa accattggga gggcggcacg ccatattcat
     2521 ggtacggcga tattgtatgc ggatcgtatt actaattcga tgcgtcgtgc gatcgatgaa
     2581 acggaacgga ggcgtcagaa gcaattatta tttaatgaag cgcatcacat tacgcccaaa
     2641 ggggtcagta aacgcatcaa agatttgatc gatggcgttt attgcagtga aacagcgcaa
     2701 aataatctcc gtgcggcaca agcagaagca cgttacgaga atatgagtga atcccgaatg
     2761 gcgcaagaga tcaagcgtct cgaaaaacag atgctagcca cagcaaagaa tcttgaattt
     2821 gagaaagcgg ctgaattacg cgacgaaatc aaaaagctaa aagacaagtt gtttgtgggt
     2881 tatgtcgaac ctgaaataaa cccatcgaat accaataatg cacaacccct ggcaatgaac
     2941 cctaagcaag gggttatacg gcgagtcaaa aaacagaaat aaccgttatt gcgccagctt
     3001 aacttgagtg gacgtgacaa aaatgatgcg atcaaacgtt ttattcaatg ttttactggt
     3061 gaaattatgc atcgacatac tctcgacaaa gcgaatatgg tcgccttttt ttacctgggt
     3121 cgtgggggct gctatccaaa aacgcttgct cccatttccc agctccatat aagtgtatcc
     3181 tgtggtatca agcgttgata tcacaatgcc ttcattctga ggtaagggct gagcaggttg
     3241 ctcagcatac ccttgtgtag aaatgaacac gaacaatgaa aaaataatac taacaagtag
     3301 gcgacgcata acgattaaca atatcctcgt attagaaacg aataacgtat tgtgcgtaga
     3361 agaaatcagg tgaaatgcct ggtctttgcg ctcttaaaaa atcacctgca aatagtttgg
     3421 aataaccaaa caagacatct tgatgacgac tcacgtggac attaacccgg aagtctattt
     3481 catctcccac atggctacct gattgcccag ttggatcacg caatgttgca agccctgccg
     3541 cgttatataa gaaatcacgc gtattcgcta aatagaagcg atgatattgt gcgatgaagg
     3601 taaaccacgg ttgcggatgt aaggtaaatt gcgcattaaa gtcgtgaatg ttttgacgac
     3661 ctacttgatc caagaaaccc atgtaataat gcccaaatgg gaataaatga ttaaaggtat
     3721 tcgatcttcc atcttggctg tttgcatcac ctgaagcaaa atcataacga atccagaatt
     3781 gaggattcat cggtaacggc acgtggtaac ctaacccagt tgcaactgca aaagccgata
     3841 tatcgaggtg tgaacgacga ccaaactgat acatcccttc aaactcgtaa agaaagcggt
     3901 tgtaatcacc ggcaacacgt ccaccaaggg tatgcagcac agaatcccct tgcagaatat
     3961 tcccttgttg cacggtgaag ggattcagga tacggttatc gaccaaactc aagtaataga
     4021 gatcaagcaa ctgccctttc attggtttat aggttcccca aagacccatg aaattcctac
     4081 ctttatccca attatcgaat tcattacgtt cgatcgtcat cggacgaacc cagaatgcgt
     4141 ctaaatcaaa cgttggggta cgccagaacg ctttaacccc ttggaaggta cggcgcgtat
     4201 taacccactc gagcggtgaa atcaagcgtt gcgaaccata cagtaattct tgacgcccaa
     4261 cccgcatgta tactggtcca tcgacgagtt ggccgagttt aacgtcagca aataaattga
     4321 gtatatccgt ataattccga tctgtcgtca gcgcaggtaa atcaagaccc aacgcgcgcg
     4381 catcgatgaa ttcagcaaat aatctaaatt gatcgcgata ccaaaggtca gcatggaaac
     4441 gagtacggaa aagatgatat ttattatcct gcccccccgc atttaagcgg ctatcggttt
     4501 cgtgcatgta gcgataccag aattgcccac cgaatgacag catccagtta tcagccagtt
     4561 ttatgcgttt gaacggatcg aaaacatcct tttcatgatc tggtttttcc agatagcgaa
     4621 aatcaacatc aaacccagaa gtaggtagca gtccaaaggg tgcataaggt gctaccggcg
     4681 cagcttcacg tttattgcct gagatgagat cccataatga aaaataacca ggtcctgccg
     4741 gtggaatcac aaataatccg gggcgtggca ttggcgttac cggtggaacg cctccccaat
     4801 taaacttttc aggtgctgca ttcggtgcag cgggtgccgc tgtattggtt gtatttgatg
     4861 gattcgctga tgctgcttta gcgggctcgt tcgccaacga ctgaaaggtg cagcttatgg
     4921 ttgcgatcgt taaaaatcta tttatccaat attttttacc aaaaagattc atgaattacc
     4981 tccccaaggt atttgataaa cccataataa gcgttcatat atgaacagca tctataattt
     5041 atataatttt aattttggcg cattctagca aaactaggca aaatccggtg aattgaatcg
     5101 cgcgagtgtc tcttttttat ctattacgat atgaagtgat gaatggggag atgttataac
     5161 tcggttcatg cctgttcgat ttatgcaata gcggggtgat cctcagttat tatatggagt
     5221 aggtaaaaat ctataggcta gctttattga tcaatgttga atgagtccga aaaaaatcag
     5281 gtatcgcctt ggtatcagtt gaatactgag ggaggtattc attcattaaa attgacagga
     5341 agctataccc ttggagcact gaataatgaa atcaagaaac tcggcactcg attagaatat
     5401 tttgccaata aacccaatgt tcaatgggat ttaaccggga ttgaaacgct tgatcatgta
     5461 ggattggcaa tgatttggcg ttcttgggga aagcagcgtc cttcccaggt tttgcttcga
     5521 atagaccaag agaaattgtt tgatcattta gcacaatcgt cacaagcatt agctaagccg
     5581 aaatggcaag cggatttgct gtggcctatc gtttttctgg ggaagcgttt tttcctattc
     5641 tggaatcact tgacgggcat tattacgttg ttagggcagg tcattttaga tttattctat
     5701 ttggttcgac atcctcggca aattccgtta cgcgaaattt ctgcgaattt atatcgaact
     5761 ggcgcgcaag cgctcgggat tacagcagtt gtcggttttc taattggcat tgttttgagt
     5821 tatttaatgt ctcaacagct ccacacattt ggtgcggatg tgtttatcgt gaatattctc
     5881 ggcatgagca ttattcgtga attagggccg atgttggcag caattctggt cgcaggccgc
     5941 tcaggttctt cgatgactgc acaattgggg gttatgcggg tgactgaaga gctcgatgca
     6001 atgacggtaa tgggtattcc tcatagcatg cgattggtgc ttcccaagat tattggtctg
     6061 ggtattgcca tgccattggt cgtgctttgg accagtgcat ccgcattgtt gggtggtatt
     6121 cttgctgcag atttgcaaat tgggctcagt tatcaatatg ccttgatgaa cctacctaat
     6181 gcagtaccgg ttgctaatct ctggatcggc ctgggtaaag gtttggtttg tggcatggcg
     6241 atcgcgctca ttgcatgcca ttatgggctg cgcatcaaac ctaataccga aagcttaggt
     6301 gaaggaacta ccaactcagt ggtgacctcg atcaccgcag tcattttgat tgatgcgatt
     6361 tttgcaatat tattttcaaa tgttggactc aggttttctc ggtgatgatg gagtctggtt
     6421 ctattattga aatcaatgga ctttataccc agtttggtcc gcatcttata caccgtaata
     6481 ttaacctcaa tgttcttaaa ggtgaggttt tgtcattggt gggtgggtca ggaagcggta
     6541 aaactacatt gctgcgacaa atgctcggtt tggaaacgcc ctcgcagggg agtgtgaaaa
     6601 tttttggctg ctctcgctat gcgtgtaacc ttgatacttt gaaagctatt cgccatcgat
     6661 caggcgtatt atttcagcaa ggtgcgttgt ttagtgcatt gactgtattt gataatatag
     6721 cgctgccgat gcgtgaattg cattgtctgg atgaagcttt gattcatgat ttagtgatgc
     6781 taaagctgaa aatggtggaa gtgggcgcag aacacgccaa taaacgacct gctgaactct
     6841 ctgggggtat gatcaagcga gttgctctag cccgggcgtt agcgcttaat cctgaattac
     6901 tttttcttga tgaaccgacg gccggactcg atccttcatt aagtgaaagt attgtggatc
     6961 ttgttcgagc gctgagggaa gaactaacgc taaccatcgt catggtgacc catgatctcg
     7021 atacgttggc tgcgatttca acccggatcg cagtattggc agatcagcat attgttgcgt
     7081 tgggaaagtt acaagaggtt gttcaggttg atcacccgtt tatcaaggat tttttctggg
     7141 gtgaacgagg acaaaatgcg ttgcgcaaat atttaattgc tgattagcag ctagcgcttc
     7201 gctaaaaatg aatcgtacgg ataaggtcta tcggatttca gattatttaa agtaaagctt
     7261 aaagcgaaca taaggaaaat aaatgtcagg tgatacgctt acactcgctc aaatgttgat
     7321 ttctcgacgt tcattgacac cgcgcgatga tgggtgtcag aaactgctag aaaaccgttt
     7381 gaaagcatta ggatttcaga tcgaaaaatt aacttttggg gatgtcgaga atttatgggc
     7441 gcgtcgcggt acaaagcaac cattagtgtg ctttgcaggg cataccgatg ttgttcctac
     7501 agggcctttg ggtgagtggg aaagtgatcc gtttgtgccg accattcggg ataaccgatt
     7561 gtatggacgg ggtgctgccg atatgaaggc ctcattggct gcctttatta cggcgattga
     7621 atcg
//
LOCUS       DAOQKQ010000128         7613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_4715_length_7613_cov_6.234454, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000128 DAOQKQ010000000
VERSION     DAOQKQ010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 7613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7613
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4715_length_7613_cov_6.234454"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="PKZ49_08940"
     CDS             complement(<1..1136)
                     /locus_tag="PKZ49_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HND36727.1"
                     /translation="MAQLTFLGGTNEVTGSCYLVETEETRFLVDCGMFQGGSEAEIKN
                     RSAFSFDPETIDFVLLTHAHIDHSGLLPRLSARGFRGPVYTTGATIDLLKVMLKDSAY
                     IQEKEVEWKNKILQRKQHVSEYENAPLYTVTQAEIFLKQLCSIAYDEKVFPHPTIQCC
                     FRDAGHILGSAIVEVWIKTQSKQKKLVFSGDIGQPGHPVVQDPTPIYQADVLLVESTY
                     GNRLHRSMVDTIDELTYAVNDTLLRKGGNIIVPAFSVGRTQELLFLLIDLHRQNKLGK
                     MDIYVDSPMAKAATDITLQHSQLLDKEAHETLQWFNKEGNPLKINFTENVEDSIALNA
                     IDHGAIIISASGMCDAGRIKYHLKYNLPRPECTILITGFQAAGT"
     gene            complement(1154..2467)
                     /gene="bioA"
                     /locus_tag="PKZ49_08945"
     CDS             complement(1154..2467)
                     /gene="bioA"
                     /locus_tag="PKZ49_08945"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HND36728.1"
                     /translation="MNDKNLSIRSFKHIWHPCTQMKHHETSPLVPIEKAQGIWLYDTN
                     GNRYMDAISSWWVNLFGHCHPYINDALVDQLNKIEHVMFAGFTHQPAVELAERLAQIT
                     PGNLDHCFFASDGASATEIALKMSFHYWQLYGKTDKVNFISLKNGYHGETLGSLSVTE
                     IPFFRQTYAPLLRNTEYMPSPDWRNIIGNESHLEFALKAASELEIFLSKHHTQIAAFI
                     LEPIVQAAGGMGMYHPIYLQRARTICDKYQVHLIADEIAVGFGRTGKMFACEHAEIVP
                     DLLCLAKGLTGGYLPLSVVMTTDKLYQAFYQDEISKAFVHSHSHTGNALACRASLATL
                     DLIEQYNIIEANQAKIDYLNQLASPLQSHPKVKNFRNRGMIWAFEVETNDSKFSNNFF
                     QKALEKQLLLRPLGKTVYFMPPYIIKEREIEHLVSNVLQIIDMQS"
     gene            2634..2924
                     /gene="groES"
                     /locus_tag="PKZ49_08950"
     CDS             2634..2924
                     /gene="groES"
                     /locus_tag="PKZ49_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HND36729.1"
                     /translation="MNIRPLHDRVIVKRLEEERKTASGIVIPDSATEKPDQGEILAVG
                     KGKAGDDGKIRPLEVKVGDKVLFGKYSGQAVKIKGEEYLVMREEDIMGVIEG"
     gene            2984..4627
                     /gene="groL"
                     /locus_tag="PKZ49_08955"
     CDS             2984..4627
                     /gene="groL"
                     /locus_tag="PKZ49_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110680.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HND36730.1"
                     /translation="MAAKEVRFGDVARHNLVHGVNILADAVKVTLGPKGRNVVLERSY
                     GAPTITKDGVSVAKEIELKDKFENMGAQMLKEVASKTSDVAGDGTTTATVLAQSIVKE
                     GMRYVAAGMNPMDLKRGIDKAVIATIEDLKKLSKPCSTSKEIAQVGSISANSDAEIGK
                     IIAEAMEKVGKEGVITVEDGSGLQNELEVVEGMQFDRGYLSPYFVSSAEKQIALLESP
                     FVLLHDKKISNIRDLLPVLEQVAKAGKPLLIIAEDVDGEALATLVVNNIRGILKTCAV
                     KAPGFGDRRKAMLEDIAILTGGTVVAEEVGLSLEKVKLDDLGQAKRIEVGKENTTIID
                     GAGDVKSIEGRVAQIRTQIEEATSDYDKEKLQERVAKLAGGVALIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGIIPGGGVALMRAIGTVGKLKGDNHDQDAGIKIVERALEEP
                     LRQIVANCGDEPSVVANKVKEGKGNFGYNAATGEYGDMVAMGVLDPTKVTRSALQNAA
                     SVAGLILTTDAMVAELPKDESASPAPGMGGMGGMGGMDM"
     gene            4723..5475
                     /locus_tag="PKZ49_08960"
     CDS             4723..5475
                     /locus_tag="PKZ49_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HND36731.1"
                     /translation="MENFSNYQADKDLLRDRVILVTGAGQGIGRQAAITYAACGAIVI
                     LHGRKTKKLENVYDEISELKREEAVIFPFDFEKATENDFYTIAESIASQLGRLDGILH
                     NAAFTYGPMPFEHHTLEHLQTLMHVNLLAPITLTKACLPLLKLSNDASIVMTSDDHGH
                     VPSAFWGAFAIAKAGVEAYVKIQSEEWTLLPNIRINGLVPGPVHSPQRVKTHPGELKS
                     KLLQPSDLMGTYLYLMGPDSKGRSGEVLFCQS"
     gene            5547..5631
                     /locus_tag="PKZ49_08965"
     tRNA            5547..5631
                     /locus_tag="PKZ49_08965"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5581..5583,aa:Leu,seq:tag)
     gene            5653..6963
                     /gene="tig"
                     /locus_tag="PKZ49_08970"
     CDS             5653..6963
                     /gene="tig"
                     /locus_tag="PKZ49_08970"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HND36732.1"
                     /translation="MQSNTESPNALERKISISIPQEDVRVEVDNRLKKLSGKVKLPGF
                     RPGKVPLKMVTQQYGLQLHQEVLSELVQKHFRDSLQKQDMRIAGTPNFSSKSLDGDSL
                     NYEFDAVFEVYPEITIGNFSDLNVSKPGVQIGEAEVEKTLDTLRKQRAKFESIDRPAG
                     KGDRVNIDYHGQVDGKEFEGGQAKGFSLILGDGYALKDFEDAIIDMKNGENKVFQMIF
                     PTDYHGKEVAGKSVTFDVRLNKVEEQLLPSIDSDFAKSLGISDGDINKMYAEVKVSIE
                     REVLQRIRTKLKEQVMQFLLESTNCGIPKVLVNHEIEHLLQEANNNLKARGLADREAN
                     LSPDLFRERAERRVKLGLILAKLIEQQGIKVQSDQLRKYVEEYAKSYENPEQVINWYY
                     SSPERLKDIESVVLEDNVVTWILERAHITEQQMSFDDLMGYSKH"
     gene            7009..7584
                     /gene="clpP"
                     /locus_tag="PKZ49_08975"
     CDS             7009..7584
                     /gene="clpP"
                     /locus_tag="PKZ49_08975"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HND36733.1"
                     /translation="MVIETSGRGERAYDIYSRLLKERIIFLVGPVTEVTANLVVAQLL
                     FLESENSDKDIYLYINSPGGSVSAGLAIYDTMQFIKPNVSTLCIGQAASMGALLLTAG
                     EKGKRFCLPNSRVMIHQPMGGFQGQASDIEIHAKEILYLKSKLNEIMSKHTGQSVKVI
                     EKDTDRDNFLSADEAVKYGLVDAVLTSRVVG"
BASE COUNT         2366 a         1287 c         1745 g         2215 t
ORIGIN      
        1 aatgttccgg cggcttgaaa accggtgatc agtatcgtgc attcaggtct aggaaggtta
       61 tatttaagat gatatttgat acgacccgca tcgcacatac cactcgctga aatgatgata
      121 gccccgtgat caatcgcatt gagcgcgatg gaatcttcaa cattctcggt gaagttgatt
      181 tttagtgggt ttccttcttt attgaaccac tgtagggttt catgcgcttc tttatccagt
      241 aattggctat gctgaagtgt aatatctgtg gcagcttttg ccattggcga atcaacataa
      301 atatccattt ttcctaactt attttgtcga tgtaaatcga tgagtaagaa aaggagttct
      361 tgagttcttc caacagaaaa agcaggcacg atgatgttac cgccttttct taatagcgta
      421 tcgttaacgg cataggtaag ctcatcgatg gtatctacca tagatcgatg aagtcgatta
      481 ccgtaggttg attcaacgag tagaacatct gcttgataaa tcggggttgg gtcttgcaca
      541 acgggatgtc ctggttgtcc aatgtcgcct gaaaagacta atttcttttg ctttgattga
      601 gtttttatcc agacctcgac aatcgcggat cctaagatat gtcccgcatc acgaaagcag
      661 cactgtatcg ttggatgagg aaaaactttt tcatcataag ctatactgca caactgttta
      721 agaaagattt ctgcttgggt aactgtgtat aaaggcgcat tttcatattc agaaacatgt
      781 tgttttcttt gcaggatttt atttttccat tccacttctt tttcttgaat ataggcgcta
      841 tcttttagca taaccttgag tagatcgata gtggccccag tcgtatagac cggtcctcta
      901 aaccctcgag cgcttaatcg tggtagtaat ccagaatgat caatatgcgc atgggtgagg
      961 agcacgaaat caattgtttc aggatcgaag ctgaatgcag atctattttt aatctctgcc
     1021 tcacttcccc cttgaaacat tccacaatca actagaaatc gagtttcctc agtttctacg
     1081 aggtaacagg aacctgttac ctcattcgtg ccacctaaga aagtaagttg tgccaaggta
     1141 tttcctattt ttactacgat tgcatgtcaa taatttgtag aacattgctt actaaatgtt
     1201 caatttcacg ttctttaatg atgtatggtg gcataaagta tactgttttt ccaagtggtc
     1261 gaagcagtag ctgcttctcg agtgctttct ggaaaaaatt attcgagaac ttggagtcat
     1321 tcgtttcgac ttcaaaagcc cagatcatgc cacgattacg aaagtttttt actttagggt
     1381 gggactgcag tggtgaagca agttgattga ggtaatcaat tttcgcttga ttcgcttcaa
     1441 ttatgttgta ttgttcgatc aaatccagtg tggcaaggga tgcacggcat gccagggcat
     1501 ttccagtatg agaatgggaa tgaacaaacg cttttgagat ttcgtcttga taaaaagcct
     1561 ggtatagctt atcagttgtc atgacaacgg aaagtggcag atagcccccc gttaatcctt
     1621 tcgccaaaca taataaatct ggaacaattt cagcatgttc gcacgcaaac atttttcctg
     1681 tacgaccaaa cccaactgca atttcatcgg cgattaaatg aacttgatat ttatcacaaa
     1741 tagttctagc ccgttgtaag taaataggat gatacatacc catcccgcca gctgcttgaa
     1801 caatcggttc gagaataaat gcggcaattt gtgtatggtg cttggaaaga aaaatctcta
     1861 attcagaggc tgcttttaat gcgaattcta ggtgtgattc attgccgata atatttcgcc
     1921 aatcgggtga aggcatatat tcagtgtttc gaagcagtgg cgcataagtt tgtctaaaaa
     1981 atggaatttc ggtgaccgat aatgaaccga gcgtttcccc gtgataacca tttttcaagc
     2041 tgatgaaatt gactttgtca gtttttccat ataattgcca atagtgaaag ctcattttta
     2101 gagcaatttc agtagctgat gccccatcgc tagcaaaaaa acaatgatct aagttacctg
     2161 gcgtaatctg agccaatctt tctgctagct caacagccgg ttgatgagta aaaccggcga
     2221 acatgacatg ttcaatttta ttcaattgat caactagcgc atcattgata taagggtggc
     2281 aatgcccaaa aagatttacc caccaagaac taattgcatc catataacga ttcccatttg
     2341 tatcataaag ccagatacct tgagcctttt cgataggtac aaggggagat gtctcatggt
     2401 gtttcatctg agtgcacgga tgccagatat gcttaaaact acgtattgat aaatttttat
     2461 cattcatcga cttgaaattc ctgtgaatag atcatattat tagcactcgt cagtgttgag
     2521 tgctaattac tttaaaatag cgaatactgc tacaaaacca gcatgagttt tgattgaatt
     2581 gatttgattt taagtatgtt catgtttttt aatcattatt aggagaatca actatgaata
     2641 ttcgcccctt acacgatcgt gttattgtca agcggctaga agaagaacgc aagacggctt
     2701 cgggaattgt tattccagat tctgcaacag agaaaccaga tcaaggtgaa attttagctg
     2761 ttggaaaggg aaaagctggg gatgatggga aaattcgccc tttggaagta aaagttggtg
     2821 acaaagtttt gtttggaaaa tattccggtc aagctgtaaa aatcaaaggt gaagaatacc
     2881 ttgttatgcg tgaagaagac atcatgggtg ttattgaagg ttaaggtatt taatcctttt
     2941 aatttgaaat ttgatcatgc aacagtaatt taggagaaga attatggcag caaaagaagt
     3001 tagatttggt gatgtagcac gtcataattt ggtacacggg gttaacattc ttgcagacgc
     3061 agtcaaggta accttgggtc caaaaggtcg taatgtggtg ctggagcgtt cctatggtgc
     3121 accgactatt actaaagatg gtgtatcggt agcaaaggaa attgaactta aagataaatt
     3181 cgagaatatg ggtgcccaga tgcttaaaga ggttgcaagt aaaacctctg atgtagcagg
     3241 agatggtaca acgacagcaa cggtattagc acaatcaatt gtgaaagaag gaatgcgtta
     3301 tgtcgcggcg ggtatgaatc caatggattt aaaacgcgga atcgataaag cagttatagc
     3361 aaccattgaa gacctaaaaa aactttctaa accctgttct accagcaaag aaattgctca
     3421 agttggcagt atttccgcaa attcagatgc ggagattgga aaaatcattg ctgaggcaat
     3481 ggaaaaagtt ggcaaagaag gtgtaattac ggttgaagat ggctcaggtt tacaaaacga
     3541 gcttgaagtt gtagaaggta tgcaatttga tcggggttat ttatctcctt atttcgtcag
     3601 tagtgcggaa aaacaaattg cattgctaga aagtcccttt gtattgttac atgataagaa
     3661 aatttctaac attcgtgatt tgttacccgt tctagagcaa gttgcaaaag cagggaagcc
     3721 tttattgatt atcgctgaag acgttgatgg agaagcgctt gcaacattag tggtcaataa
     3781 tattcgcggt attctcaaaa cttgtgcagt taaggcaccc ggttttggtg atcgccgtaa
     3841 agcgatgctg gaagatattg cgatattgac cggtggaacg gtagttgccg aagaagttgg
     3901 attatcgctt gaaaaagtta aattggatga cttaggtcaa gccaagcgaa tcgaagttgg
     3961 aaaagaaaat accactatta ttgatggtgc tggtgatgtg aaatcgatcg aagggcgtgt
     4021 agcgcaaatt cgtacacaaa ttgaagaagc aaccagcgat tacgataaag aaaaattgca
     4081 agaacgtgta gcaaaacttg ctggtggcgt tgcattgatt aaagtaggtg ctgcgacaga
     4141 agtagaaatg aaagagaaaa aagcccgagt ggaagacgca ctccatgcaa cccgtgcagc
     4201 agtggaagaa ggtatcatcc ctggcggtgg cgttgcgcta atgcgtgcta ttggtactgt
     4261 aggcaaactt aaaggtgaca atcatgacca agatgctggg attaagatag tagaacgagc
     4321 attagaagaa cctttgcgtc agattgttgc aaactgtggt gatgaaccat cagttgttgc
     4381 aaacaaagta aaagaaggta aaggaaattt tggatataat gcagcgacag gtgagtatgg
     4441 tgacatggtc gcaatgggtg tattagatcc tactaaagtt acccgttcag cgcttcagaa
     4501 cgcagcttca gttgcaggac ttatcctaac aaccgatgca atggtagcgg aattgccaaa
     4561 agatgaatct gcttcacctg ctccgggtat ggggggcatg ggcggtatgg gcggcatgga
     4621 tatgtaacgc cgttattcag aaaacgacga gtaatctatc cctaatcgct acaacaatag
     4681 gtcttcaagg cttgttgttg tggcgatatt ttttattgat atatggaaaa ttttagtaat
     4741 tatcaagctg ataaagattt attaagagac cgggtcattt tagtaacagg tgcgggacag
     4801 ggaattgggc gccaggccgc aataacttat gctgcttgtg gagcaatcgt tattcttcat
     4861 ggtcggaaaa ctaagaagct tgaaaatgtg tatgacgaaa tcagtgagct aaagcgggaa
     4921 gaagcagtta ttttcccctt tgattttgag aaagcaactg agaatgattt ttatacaatt
     4981 gcagaatcga ttgcgtcaca attaggaaga cttgatggaa ttttgcataa cgctgctttt
     5041 acttacggcc caatgccttt tgagcatcat acacttgagc acttgcaaac attaatgcat
     5101 gttaatttgc ttgccccgat aacactaaca aaagcatgtt tgcccctact caaattatct
     5161 aatgatgcga gtattgttat gacatctgat gaccacggtc atgtgccttc agccttctgg
     5221 ggggcatttg ctatcgcaaa ggcaggggtg gaagcttatg tgaagattca atctgaagag
     5281 tggaccttat tgccgaatat tcgtattaat gggcttgttc cagggccagt tcattcacca
     5341 cagcgtgtta aaacgcatcc tggagaactg aaaagtaaat tgttgcaacc tagtgattta
     5401 atggggactt atctttattt aatggggcct gatagcaaag gtagaagtgg cgaggtacta
     5461 ttttgtcaat cctagagtcc tttggctgct atcccaacca taacaaggta taattcacaa
     5521 acgaaaaaaa ttaattagtt gaaaaagcga aagtggcgga attggtagac gcaccagatt
     5581 taggttctgg cgccgtaagg tgtgggggtt cgagtccccc ctttcgcacc aaacttgttt
     5641 aggaagagtc caatgcaatc aaatacagaa agcccaaacg cgctcgaacg aaaaatcagc
     5701 atttctatac cccaggaaga tgttcgcgtt gaggtggata atcgtttaaa aaagctatca
     5761 ggaaaagtta aattgccagg tttccgtcct ggcaaggtgc cattgaaaat ggtgacgcag
     5821 cagtatggtc tacaattgca ccaggaagtt ttgagtgagt tggtacaaaa acattttcgt
     5881 gattcattgc aaaagcaaga tatgcgaatt gcaggtacgc caaatttcag ttctaaatcg
     5941 cttgatggag atagcttaaa ttatgaattt gatgctgttt ttgaagtcta tccagaaatc
     6001 acgattggaa attttagcga tcttaatgtt tctaaacctg gtgtacaaat aggggaagct
     6061 gaagttgaga aaacattgga tactttgaga aagcaaagag caaaatttga atcaatcgat
     6121 agacctgcag gaaagggtga tcgggtaaat attgattatc atggacaggt cgatggaaag
     6181 gaatttgaag gaggacaggc aaaaggtttt agcctaatat taggcgatgg ttatgcatta
     6241 aaagactttg aagatgccat tattgatatg aaaaatggcg aaaataaagt atttcaaatg
     6301 atatttccta cagattacca tggtaaagaa gttgcaggta aatccgtaac ctttgatgtt
     6361 agattgaaca aagtagaaga acaattactt ccaagtattg acagtgattt tgctaagtca
     6421 cttggtatca gcgatggaga cattaataaa atgtacgctg aggtaaaagt gagtattgag
     6481 cgtgaggtat tacaaagaat tcgtactaag ctaaaagagc aagtgatgca atttttactt
     6541 gaaagcacaa actgcgggat acctaaagtg ttagtaaatc atgagattga gcacttactt
     6601 caagaagcta ataacaatct aaaggcacga ggtcttgctg atcgagaggc caatttatcg
     6661 cctgatttat tccgagaaag agctgaacga cgtgttaagt taggtttgat tttagctaaa
     6721 ctcatagaac aacaaggtat aaaagtacaa tcagaccagt taagaaagta tgtagaggaa
     6781 tatgctaaaa gctatgaaaa tcctgaacaa gtcattaatt ggtattattc atcgcctgaa
     6841 cgattaaaag atatcgaatc ggtagtatta gaggataatg ttgtaacgtg gatattagaa
     6901 agagcccata ttactgagca gcaaatgtca tttgacgatt taatggggta ttcgaagcat
     6961 taatttacgg gggtgaattt ggatatcaat ggattaggtt taattccaat ggtcattgag
     7021 accagcggaa gaggtgaaag agcatacgat atctattcta ggttattaaa agaaaggata
     7081 atttttcttg ttgggccagt gactgaagta acagctaatt tagttgtagc tcaattatta
     7141 tttctagaat ctgagaactc tgataaagat atttatttat atattaattc acctgggggt
     7201 tctgtctcag ctggtctcgc aatttatgac acgatgcaat ttattaaacc taatgtgagt
     7261 acactatgta ttggtcaggc ggcaagtatg ggcgcactat tattaactgc tggtgagaag
     7321 ggtaaacgtt tttgcttgcc taattcacgg gtaatgattc accagccgat gggtggtttt
     7381 caaggccaag catctgatat cgagattcac gctaaagaga ttttatatct taaaagcaag
     7441 ttaaatgaga ttatgtcgaa gcacactggt caatcagtaa aagtaattga gaaagatact
     7501 gatcgtgata attttcttag tgcggatgaa gctgtaaaat atggtttagt tgatgcagta
     7561 ttaacaagta gggttgtagg atagaatggg gcatttggcg tgcttgatac aac
//
LOCUS       DAOQKQ010000129         7575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_4741_length_7575_cov_5.611702, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000129 DAOQKQ010000000
VERSION     DAOQKQ010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 7575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7575
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4741_length_7575_cov_5.611702"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PKZ49_08980"
     CDS             <1..505
                     /locus_tag="PKZ49_08980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HND36734.1"
                     /translation="DPTGSTTGFQVSSLTNPAFGAPGQFQAFSPEKLFRTVGGDEFDI
                     RFFSPSDQVTSVFTTGFGMVFTDADLGTLPTLEFFDAQGNLLHSLTGQATGSFGLGFM
                     GTVFDTAVVSRIHVTIGDANGLVMDDFIFGEPTRQQNQIPEPSSLALMFAGAFYLTRK
                     LTRQAFN"
     gene            complement(582..761)
                     /locus_tag="PKZ49_08985"
     CDS             complement(582..761)
                     /locus_tag="PKZ49_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36735.1"
                     /translation="MHNSNLGRVIKRRTLSSFLVIAIQNVADSSTNTGDQLLAQINQP
                     VESIGVDYSCFVAEK"
     gene            955..2676
                     /locus_tag="PKZ49_08990"
     CDS             955..2676
                     /locus_tag="PKZ49_08990"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HND36736.1"
                     /translation="MTHQNMQIIEASSPVYSEHQKEFYRRLQYWCLFISVKVILLSGL
                     LLFSINAASADEWIYRMQQGDNLWNLTERHLTHLKFVLPLQRLNHIQNPFQIPPGTEI
                     RIPIQWTRQQSSSALVLRVFGDAQLRRQGLAQPLPVVDGMQIFPGDAISTGEDAYVTF
                     EFADQSILRLQDNSELRFERLEILGNSAVISTEVNLQKGRTESQVPKHSPINSRFRII
                     TPSAVSSVRGTDFRVGANNDRATMNEVIGGLVQVSNQHQSINIPSGFGVLAEANSKSL
                     QSVPLLPPPDLSETPSLYERILLNIPVRPLSGASGYRAQIALDESFTQLITDFDTASL
                     PIRGGELPDGDYWLRIRGRDSLGLEGFEAKLPIRLNARPEPPFIIAPQHDSVVENQSP
                     ELQWAHQPEVAHYIIAITQKDSDFSTPIIYEPEVKGNRYQLNQSLEPGDYVWKIAAVS
                     AVEGIGPYNDPLSFRVPVPGPALESTDVSDQNISFSWAATEPGQYFHFQFARDEDFKQ
                     PIVDTFIHESKITLPRQDSGRHYLRIKIIEADGTHGSFSPAQMIEIPSKYPYWLFLLL
                     PLIGLLL"
     gene            2724..5549
                     /locus_tag="PKZ49_08995"
     CDS             2724..5549
                     /locus_tag="PKZ49_08995"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HND36737.1"
                     /translation="MIIVVACLPFTGWLERFDWMIYDKISYWKQFIPDDQIVIIAIDE
                     KSLQTIGRWPWSRRIHAELIDRLRQAGRNVVALDFLMAESDDNDPEGDRLLGLAIARH
                     GAVVLPIAPIVEPDSQKISLVQPLPMFQRNALLGHVDVELDYDGVARRLFLFAGVNSP
                     DWPALGYVLVRKAGGINAVSDSDYLIRSEINIPGTSDYWVRSHEVLIPFAGSPGTYPR
                     ISYERILNDDLVLKQLNNKIIIVGMEAIGLHSGFTTPVTSNHRLMSGIEWHANVVNML
                     HSGNTINPLAKFWIVFISIIWVLIALIGVNFIQRNKVIFLLMTVMALGMLLVWIMFDF
                     FHVWIAPSAGLIGLILVYPLWSWSRINEFMQSLFITKTFSFNALESVGDGVITTDAES
                     RIVYMNSEAERLIGIGIEQLKGSLLYELLDIKVVRRSRSIEIREISTLVKNIPQLQDQ
                     YRLKNYDESKRMVRVMHQSLFDEHKVLMGSVVTISDITDTVELKQQVSHQAHHDSLTM
                     LPNRTLLLAKLDQMVESAQQTTSIFAVFNIVIDEFKKINDALGHRAGDSLLKLVTQRL
                     QAIIGEQNVIARWSGDEFMVLFASAQDALQLVPNVAQDIIDSISHRFELDGQEIFVTA
                     SIGISLYPQDGQTGESVLSHAASAMGLAKQHGGNQFSFYLPDKVTDWTKDKLALEKDF
                     RLALKNNELDVFFQPIINVQLRRVVRMEALVRWIHPIRGFLPPGDFVPMAEQIGLINQ
                     MGELVLYKACKLARELEKIAGPISISVNVAPRQLLQKGFIQIVETIINEVGLPAELLV
                     LEITESAIIGDMVLASEILSDLKSLGILIALDDFGTGYSSLSLLRELPIDILKIDKSF
                     IRTIDGNVHDFTIAQTIIGLGKNLGKTVIAEGVETANHMKILRDHACYLQQGFYFSRP
                     LPFAQLIRYFEKNELAVATLCEYFPTN"
     gene            5605..6099
                     /gene="creA"
                     /locus_tag="PKZ49_09000"
     CDS             5605..6099
                     /gene="creA"
                     /locus_tag="PKZ49_09000"
                     /inference="COORDINATES: protein motif:HMM:NF008026.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HND36738.1"
                     /translation="MMQLLNRYRNFGFIFMGIALAILPLAAVAEQIGSVSTKFKVLGP
                     NDKIVIEAFDDPDIKGATCYLSRAKTGGISGAVGVAEDTADASIACRQIGPIQLSDEV
                     RNGKKDGDEVFKKSTSLLFKSLQVVRFYDAERNVLIYLAYSDRIIEGSPKNSISIIPI
                     MTWR"
     gene            6239..7492
                     /gene="hemA"
                     /locus_tag="PKZ49_09005"
     CDS             6239..7492
                     /gene="hemA"
                     /locus_tag="PKZ49_09005"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HND36739.1"
                     /translation="MQLFAFGINHNTAPLNIREQIAFPQDVMEHALHDLVEHQAVKEA
                     AIVSTCNRTEIYCSTDKPDAAIHWLTDFHHLPARELDPYLYKFPREQAVKHAFRVASG
                     LDSMVLGEPQILGQMKNAIKSAESAGTLGLLLHKLFQRTFFVAKEVRTSTEIGACSVS
                     MAAAAARLAERIFEEISEQSILFIGAGEMIELCASHFAARSPKRITVANRTVERAESL
                     AKRFNGYPISLSELPEQLALHDIVVTSTASPLPILGKGMLERAIKLRKHRPIFIVDLA
                     VPRDVEPEVAELDDVFLYYVDDLADIVKEGLDARLGAVAQAETIIETNVSDFMRWMAT
                     RELVPTIRALRDQAERFRRHELARASKLLAKGEDPVKVLESFSNGLTNKFLHLPSNAL
                     NHANDQERDQLVELINRLYQLHRPQ"
BASE COUNT         2076 a         1414 c         1780 g         2305 t
ORIGIN      
        1 tgatcctacg ggttctacaa ctggcttcca agttagttca ctaactaacc cagcttttgg
       61 tgcgccaggc cagtttcagg cattttctcc tgaaaaacta tttcgaacgg tgggtggcga
      121 tgaatttgat atccgattct tttctccgtc agatcaagta acatctgtat tcacaacagg
      181 attcggaatg gtttttaccg atgcagatct tggtactcta cctacattag aattctttga
      241 tgctcaaggt aatctgttgc actcactgac agggcaggca accggaagtt ttggtcttgg
      301 atttatggga acagtattcg atactgcagt tgtttcccgc attcatgtga cgattggtga
      361 cgcaaatggc ttagtcatgg atgattttat ttttggagaa ccaacacggc aacaaaatca
      421 aattcctgaa ccaagctcct tggcattaat gtttgcaggc gctttttatt taactagaaa
      481 attaactcga caagctttca actaagagcg ggtcgatttc agtaatagct accctaaatt
      541 aggcagcaag ttaactaact tgctgcccct ccaatgttta gttatttttc tgcaacgaag
      601 caggaataat caactccaat cgattctact ggttgattga tttgcgccaa taattgatct
      661 cccgtatttg tgctgctgtc agccacattc tgaatcgcaa tgaccaagaa tgacgatagg
      721 gtacgtcttt taattactcg accaagatta ctattgtgca cagttattgg ttaataaata
      781 tactgattag gctattgtaa caagttgata aatagaacaa tatagtgctt aatcggttag
      841 agatgagaaa gtcgaagtta gaacttatgt aacgtgttat tgggaaatag ctaatattcc
      901 attgtgtttt atctgcgatg agcagaatca agatttaatc ggagttatga gttaatgacg
      961 catcagaata tgcagattat agaagcttcc tctcctgttt atagcgagca tcaaaaagaa
     1021 ttttaccgac gtttgcagta ttggtgctta tttataagcg ttaaagtaat tcttttatca
     1081 gggctgttgt tattttcaat caatgcagca agcgctgatg agtggattta cagaatgcag
     1141 cagggcgata acctttggaa tttaacagag cgacatctaa ctcacttgaa atttgtttta
     1201 ccccttcaac gcttgaacca cattcagaat ccatttcaaa tcccgcctgg aactgagata
     1261 cgaatcccaa ttcaatggac ccgccagcag tcgagttctg ctttagtatt gcgcgttttt
     1321 ggtgatgctc agttgaggcg gcaaggcttg gcccagcctt tacctgttgt agatggtatg
     1381 caaattttcc ctggggatgc aattagtact ggagaggacg cttatgtcac gtttgagttt
     1441 gcagaccaat ccattttgag attgcaagat aatagcgaat tacgattcga acgattagaa
     1501 attctgggta atagcgctgt cattagcaca gaggttaatt tgcaaaaagg gcggacagaa
     1561 agtcaggtac ccaagcattc tccaataaac agccgtttta gaatcattac accttctgcc
     1621 gtgagttcag tcaggggaac cgattttcgg gtcggagcaa acaatgatcg tgcgacgatg
     1681 aacgaagtca ttggtggttt agttcaggtt agtaaccagc atcaatcaat taatatcccg
     1741 agtggttttg gtgtgttggc agaagcgaac agtaaatcat tacaatctgt cccgttacta
     1801 cctccacctg acctaagtga aacgccttcc ctgtacgagc gtattctctt aaatattccg
     1861 gtgagaccat tatcaggggc gagcggttat cgagcccaga ttgcactcga tgagagtttt
     1921 acgcagctta taacagattt tgatactgct agtttgccca taaggggtgg agagcttccg
     1981 gatggggatt actggcttcg gattcgaggc cgtgattcgc tgggtctgga aggttttgaa
     2041 gcaaaattac caatccgtct taatgcacgt ccggaaccgc catttataat agcgcctcag
     2101 catgattcgg tcgtggaaaa tcagagcccc gagttgcagt gggcgcatca accggaagtg
     2161 gcgcactata ttattgcgat tacgcaaaag gacagtgatt tttctacacc gataatctat
     2221 gagcccgagg tgaaaggaaa tcgttatcag ctcaatcagt ctttggagcc gggtgattat
     2281 gtgtggaaaa ttgcagcggt atctgcagta gagggaatag gtccctacaa tgatccatta
     2341 tcttttcgtg ttcccgttcc aggacccgcg ctggaatcaa ccgatgtgag tgatcaaaat
     2401 atcagctttt cttgggcagc gaccgaacca gggcaatatt ttcattttca gtttgcgcgg
     2461 gatgaggatt ttaagcagcc gattgtagac acgtttattc atgaatctaa aattacctta
     2521 ccgcgccagg atagtgggcg ccattactta cgcattaaaa tcattgaagc agatggtacg
     2581 cacggttcat ttagccccgc tcaaatgatt gagattccat caaagtatcc ttattggttg
     2641 tttctcctct tgcctttgat tggcttattg ttatgaaggt gagttttgat ttttggaaca
     2701 attattcttt tcggtctgca ttattgataa tcgttgtagc ttgcttacct tttacgggtt
     2761 ggttagaacg ttttgactgg atgatttatg acaagatttc ctattggaag caatttatcc
     2821 ctgatgatca gatcgttatt attgcaattg atgaaaagag cttacagacc attggtcgtt
     2881 ggccgtggtc gagaagaatt catgcagaac tgattgatcg acttcgccag gcgggacgta
     2941 atgtcgttgc attggatttt ctcatggcgg aatcagatga taacgatcct gagggcgata
     3001 ggttgttggg attggcgatt gctcgacatg gtgctgtggt gcttccgatt gcacccattg
     3061 ttgaacctga ttctcaaaaa atttcattgg ttcagccatt gccgatgttt caacgtaatg
     3121 cgctattggg gcatgtcgat gtggaactgg attatgatgg ggtggcacgc cgactttttt
     3181 tatttgcggg cgtgaattcg cccgattggc ctgcacttgg ctatgtgtta gtccggaaag
     3241 caggaggcat caatgcagtt agtgattctg attatcttat tcgaagtgaa atcaatattc
     3301 cgggcacgag tgattattgg gttcgttctc atgaggtact cattcctttt gccggttctc
     3361 caggaaccta tcccagaata tcgtatgagc gaatcttgaa tgatgatttg gttcttaagc
     3421 agttaaataa caagatcatc atcgttggga tggaagcgat tgggttgcat tccggattta
     3481 caacgcctgt gacttcaaat catcgtttga tgagcggtat cgaatggcat gctaatgtgg
     3541 tgaatatgct gcatagcggt aatacgatta atcccctggc taaattttgg attgttttca
     3601 tttcgattat ttgggtgctg attgccctaa ttggagtaaa tttcatacaa agaaataaag
     3661 tcattttttt attgatgacg gtcatggcgc taggtatgtt actcgtctgg atcatgtttg
     3721 atttttttca tgtttggatt gcacctagcg cggggttaat tggtctaatt ttggtttatc
     3781 cattatggag ttggtcgcgt attaatgaat ttatgcaatc actctttatt accaaaacgt
     3841 tctcattcaa cgcccttgaa tcagtaggag atggcgtgat aacgacagat gctgagagtc
     3901 ggattgttta tatgaatagt gaggcggaac gattaatcgg tatcggtatt gagcagctta
     3961 aaggtagttt attgtatgaa ttactcgata ttaaagtggt gcgacgcagt agaagtattg
     4021 aaatacgtga aatcagcacg ttagttaaaa atatccctca gttgcaagac caatatcgtt
     4081 taaagaatta cgatgaaagt aagcgtatgg ttcgagtaat gcatcaatca ttgtttgatg
     4141 agcataaagt tttgatgggg tcagtggtaa cgatttcaga tattactgat acggtagaac
     4201 ttaaacagca agtttcccat caggcgcacc atgactcatt gacaatgctg cctaatcgaa
     4261 ctttgctatt agctaaactt gaccaaatgg tggagtctgc gcagcaaacg acttcaattt
     4321 ttgctgtttt taatattgtt atcgatgaat ttaagaaaat taacgatgcc ctgggccatc
     4381 gcgctggtga ttcacttctg aaactggtaa cgcaacgttt gcaagcaatc attggagagc
     4441 aaaatgtgat tgcacgttgg agtggagatg aatttatggt gttattcgct tctgcacaag
     4501 atgcgctgca attggttcct aacgttgctc aggatattat tgacagtatc agccaccgat
     4561 ttgagttgga tgggcaagaa attttcgtaa cggctagtat cggtatcagt ttgtaccctc
     4621 aagatgggca aaccggggaa tcagtattaa gtcacgctgc aagtgcgatg gggctagcaa
     4681 aacagcacgg tggtaatcaa tttagctttt atttaccgga taaagttact gattggacta
     4741 aggacaagtt agcacttgaa aaggatttcc gccttgcgct taagaacaat gagctagatg
     4801 ttttttttca accgattatt aatgttcaat tgaggcgtgt tgtccgtatg gaggcgcttg
     4861 ttcggtggat acatccgatt cgaggttttt taccgccagg ggattttgta cccatggcag
     4921 agcaaatcgg cttgattaat caaatgggtg aactcgtatt atataaagct tgtaaattgg
     4981 cacgtgagtt ggagaaaata gccgggccga ttagtatttc agttaatgtg gcaccccgcc
     5041 aattattaca aaagggtttt atccagatcg ttgagacgat tatcaacgag gttggtttac
     5101 cagctgaact gctagtgctt gaaataactg aaagtgcaat tattggggat atggtacttg
     5161 cttctgaaat actttccgac ctaaagtcct tgggcatatt aatcgcgcta gatgattttg
     5221 gaactggtta ttcttcgctt agtttactgc gtgaactgcc aatcgatatt ctaaaaattg
     5281 ataaatcatt tattcgaacg attgatggta atgtgcatga ctttaccatt gcacaaacga
     5341 ttattggtct tggtaaaaat cttggaaaaa cggtaatcgc tgagggagtt gagacagcta
     5401 accatatgaa aattctgcga gatcacgcct gctatctgca gcaaggtttt tattttagtc
     5461 gcccattgcc ttttgcgcag ctaatacggt attttgaaaa gaacgagcta gccgttgcga
     5521 ctttatgcga gtattttcca actaattgat tcgcaactaa tttatagcaa aacatcacta
     5581 aactaagatg ataaggagca taaaatgatg caattactga ataggtatcg caattttggg
     5641 ttcattttta tggggattgc actggcgatt ttgccgcttg ctgcagtggc tgaacaaatt
     5701 ggcagcgtat caaccaaatt caaagtgcta ggtcccaatg ataaaatcgt aatcgaagca
     5761 ttcgatgatc ccgatatcaa aggggcaacc tgttatctga gtcgcgccaa aacaggcggt
     5821 attagtggcg ctgtcggtgt tgcagaagat actgctgatg cctcgattgc ttgtcggcaa
     5881 ataggtccca ttcaattatc ggatgaagtt agaaacggta agaaagatgg ggatgaagta
     5941 ttcaagaaaa gtacttcatt actattcaaa tcgttacagg tcgttcgttt ttatgatgct
     6001 gaacgcaatg ttctgattta tcttgcctat agcgatcgaa ttattgaagg ctctccgaag
     6061 aatagcattt caatcattcc gataatgacg tggcgttgat aatttggctt taatcagaag
     6121 acgagttagc ttgttagcga ctgaaagaat ttaaatcgta ctttcgtcgc attgctgacg
     6181 acaaaacttt cgctataatt acgagattta tatcttttta acaacgagta gtatttccat
     6241 gcagttattt gcttttggta tcaatcataa tacagcgcca ctgaatatac gtgagcaaat
     6301 tgcttttcct caagatgtga tggagcatgc attacatgat ttagtggaac atcaagctgt
     6361 caaagaagct gccatcgttt caacctgtaa tcggacggaa atctactgta gtactgataa
     6421 gcctgatgct gcaattcatt ggctgactga ttttcaccat ttaccagcta gagaactcga
     6481 tccttatctt tataagtttc cgcgtgagca agctgtaaaa catgcgtttc gtgtcgcgag
     6541 tggactcgat tccatggtgc taggagagcc ccagattctc ggtcagatga aaaatgcgat
     6601 taaatcagca gagagcgctg gaacgcttgg attgctacta cataaattgt ttcagcgtac
     6661 tttctttgtt gcgaaagaag tgagaacttc gactgagatc ggtgcatgtt ctgtttctat
     6721 ggcagcggcg gctgcgcgcc ttgcagagcg gatctttgaa gagatcagtg agcagagcat
     6781 cttgttcatt ggagcaggtg aaatgataga actctgtgca agtcattttg cagcgcgatc
     6841 gcctaagcga atcacagtgg ctaatcgaac agtagaacgt gcagagagct tagcgaagcg
     6901 cttcaatggc tatccgattt cactcagcga attgcctgaa caattagcac ttcatgatat
     6961 tgttgtgacc agtaccgcta gtccgttacc tattttgggg aaagggatgc tggagcgcgc
     7021 gattaagctt agaaagcacc gacctatttt tatcgttgat ttagcagttc cgagggatgt
     7081 tgagccggag gtggctgaat tagatgatgt gtttttatat tatgtggatg atctggcaga
     7141 tatcgttaaa gagggccttg atgcaaggct tggtgcggtg gcgcaggcgg agacaattat
     7201 cgaaaccaat gttagtgact ttatgcgttg gatggcgacg cgtgagttgg ttccaacgat
     7261 tagagcgctt agagatcagg cagaacgttt tcgtcgacat gagctagcac gagcctctaa
     7321 attgcttgca aaaggtgaag acccagtcaa agtgcttgaa tcatttagta atggattaac
     7381 caataaattt ttgcatcttc cttctaatgc gcttaatcat gcgaatgatc aggagcgtga
     7441 tcaactcgtt gagcttataa accggctgta tcagcttcac cgtccgcaat gaacaaaagt
     7501 attgcagaac gtctcacacg tttaagtgtg agactcgaag aactaaatca acttttgagt
     7561 agtgagaagg taaca
//
LOCUS       DAOQKQ010000130         7557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_4752_length_7557_cov_5.813383, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000130 DAOQKQ010000000
VERSION     DAOQKQ010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 7557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7557
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4752_length_7557_cov_5.813383"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="PKZ49_09010"
     CDS             complement(<1..727)
                     /locus_tag="PKZ49_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36740.1"
                     /translation="MIKFLNRIYCWLDEVVLSLSREIKLSYLPPLMVYLAAGISSLTA
                     IVGTFYVKERLGLSAEFLAALGFWMALPWALKMPLGHLVDLIWRWKGFLVYLGASLIM
                     LSLLIMIGLLGYPSDMQAIMPIENWYVLAALLAPIGYVLQDVVADAMTVEAVPKVDAN
                     GQAVSQEARKQMHVTMQTLGRVAIIGGGVLVSILNVILMHDMGSLSESEKLATYLFIY
                     QLALVIPIVSVGGVFLASCLRRKN"
     gene            complement(757..1893)
                     /locus_tag="PKZ49_09015"
     CDS             complement(757..1893)
                     /locus_tag="PKZ49_09015"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HND36741.1"
                     /translation="MNKLWLIFAQTVTILLAIFFVVSTLRPELLPWTPRGKIATIKEY
                     TSANPSIIPDSFNAAAETAMPAVVNIFTSKEVRSQTHPQLDDQAFQFFFGDRFSPKTK
                     QTSSLGSGVIVSAEGYILTNHHVVEAASEIQIALVDGRNAKAKIIGSDPESDLAVLKI
                     DLENLPSITIGNSESAKIGDIVLAIGNPFAVGQTVTMGIIGAKGRSQVGINTFENFIQ
                     TDAAINPGNSGGALTDTAGNLIGINTAIWSRTGGSLGIGFAIPVSAAIQIMKQIIETG
                     SVTRGWLGVSMHDVTPELVESLKLKKLGGILITGVLKDGPADQAGIKPGDILQAVDGD
                     EISNSAEVLNLVAALPPGRTVLLTIIRSQSEKSFKVTVGKRPSS"
     gene            1961..2707
                     /locus_tag="PKZ49_09020"
     CDS             1961..2707
                     /locus_tag="PKZ49_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HND36742.1"
                     /translation="MLQYDLENYLNQLLEISRFRDYCPNGLQVEGHKQINTLISGVTA
                     SLELIEMAIACNADAILVHHGYFWKGEESCIRGMKHQRLSLLLKHKINLFAYHLPLDA
                     HLELGNNIQLARKLGLQYDGTFGEQAIAMHGQLANRMMLGDFGKTIATSLNREPLIIG
                     DLSQKVQRIAWCTGAAQGYFEEAIDARVDAFITGEISEQNVHMARESGVAFIAAGHHA
                     TERYGVQALGTHIAQRFGVNHEFIDLMNPV"
     gene            2949..4130
                     /locus_tag="PKZ49_09025"
     CDS             2949..4130
                     /locus_tag="PKZ49_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HND36743.1"
                     /translation="MKRYSFFSLLLLLALSACKTESLSTTKQADTIQPAPSVTENINQ
                     LRLTQWTQLIGWEADELLPAWNTFMQSCTTLKRQPNWQAVCTKASMLEQANEQTIRLF
                     FESQLAPYQVFNSDGSDTGIITGYYEPLLQGSKKSTEKYRFPIYSPPDDLLVIDLGNA
                     LPDFKNLQLRGRLEGRKVVPFYSRAEIEKGIPTLKNRELLWVDDRVALFFLHIQGSGR
                     IQLENGEIIKVGYADQNGHPYVSIGSLLVQRGELPLEKASMQGIKQWGVQNPEKLTEL
                     LHQNSRYVFFRLLPDSLTGPLGSLGVPLTAGRSLAIDPRVVPQGAPVFLSTTWPNSDR
                     SLNRLMVAQDTGSAIKGGVRADFFWGFGKEAENQAGKMKQNGKMWVLLPKDMTIPPHS
                     K"
     gene            complement(4141..6234)
                     /locus_tag="PKZ49_09030"
     CDS             complement(4141..6234)
                     /locus_tag="PKZ49_09030"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HND36744.1"
                     /translation="MNPLLDFTGLPRFMDIKPEHVLPAIETLLQQCRSVANQARTAAT
                     ATWNSFIQPLVDANERLSRAWGQVSHLNSVMNSPELREVYNANLPKVTQYFAELSQDQ
                     SLFEKFKQLRNSPEFNDLSNARKKIIENQLRDFRLGGAELTTDNKKRFLAIQEELSAL
                     SAKFSDNLLDATNDFYLIIAQQDELSGIPEDALLAAKEAAEKAPNPSKGWKFTLHAPS
                     YLPVMQYADNRDLREKMYRAYQTRASEIDSLVHGTSNWDNMPLINKILKLRKEEANLL
                     GFDCYAEVSLAPKMAESPRQVLDFLHELAAKAKPYAQRDLEELKVFARTKLGLEKLES
                     WDLAYASEKLRVERYAFSDQEVKQYFPENKVLPGMFRLVETLYGITISEASLSRPIQR
                     WHPDVKFFDIHDAKGQLIGQFYLDLYARPSKRGGAWMDDAMTRRRIEQENRQSSIQIP
                     VAYLTCNFSAPVNKNGQARPALFTHDEVIVLFHEFGHGLHHLLTQVEDLAVSGINGVE
                     WDAVELPSQFMENFCWEWDVLSQMTAHIESGVPLPRALFDKLLAAKNFQSGLQTLRQI
                     EFALFDMHLHYDFDPNGEKTVLTLLDEVRQQVAVVIPPAFSRFPNSFAHIFAGGYAAG
                     YYSYKWAEVLSADAYSLFEETDPSNIVNTQAGSRFWSEILAVGGSRPALESFIAFRGR
                     EPKIDALLRHNGMTT"
     gene            complement(6296..>7557)
                     /locus_tag="PKZ49_09035"
     CDS             complement(6296..>7557)
                     /locus_tag="PKZ49_09035"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HND36745.1"
                     /translation="GRLIDPQNKLDAIKDLCIAEGKIVAIGSIPTGFTDCPVIDAKDW
                     VVCPGLIDLSARLREPGLEYKATLESELDAAAAGGITSLACPPDTDPPLDEPGLVEML
                     KHRTKSLNHIHVYPIGALTQKLKGEKLTEMAELRDAGCVAFSHADEPITNLHILRQAM
                     YYASTFNFSVWLRPQEINLANRGVAHDGEVATRLGLSPIPVCAETIALSNIILLVREI
                     GVRVHVCRISSAESVAMLRTAKQQKLPITCDVSINHLHLSEMDIGFFNTNCHLIPPLR
                     NLQDRDSLRAGLIDGTIDAICSDHAPIDDDAKLLPFGESEAGASGLELLLSLTLKWTD
                     SMNLSLIDGLKVITQTPASILAIDAGHLSLGAAADICIFDPEAYWKVTPSTLKSQGKN
                     TPFMGMELPGSVKYTLVGGNIIYQRSN"
BASE COUNT         2172 a         1582 c         1646 g         2157 t
ORIGIN      
        1 tattttttct tctgaggcag cttgctagaa atactccacc gacagataca atggggataa
       61 ctagcgcaag ttggtaaata aataagtaag ttgctaattt ttctgattca gataaagaac
      121 ccatatcgtg catcagaatg acattcaaaa tcgagaccag tacccctcct ccgataattg
      181 ccactcgccc cagcgtttgc atggtgacgt gcatttgttt acgagcttct tgtgagaccg
      241 cttgcccatt agcatctact ttgggaacag cttcaacggt catcgcatct gcgaccacat
      301 cttgaagcac atagccgatt ggagcgagta atgcagctag cacataccaa ttttcgatag
      361 gcattatggc ttgcatatcg ctaggatatc ccagtaatcc gatcatgatc aacaaactga
      421 gcatgatcaa gctggcaccc agatagacaa gaaagccctt ccatcgccaa attaaatcga
      481 ctaagtgacc aagtggcatt ttcaatgccc aaggtagggc catccagaat cctaatgcag
      541 ctaaaaattc tgctgataat cctaatcgtt ctttgacata aaaagtacct acaatggcag
      601 ttaagcttga aataccagct gctaaataaa ccatgagcgg cggtagataa gataatttaa
      661 tttcacggct cagtgaaaga actacttcat caagccaaca ataaatgcga ttgagaaatt
      721 taatcaattg aattttatta ctatcgtttt agattattaa ctagatggac gcttaccgac
      781 tgtcactttg aatgattttt ccgattgact tcgaataatg gttaacaaaa cggttctacc
      841 agggggtaaa gcggcgacta agttcagtac ttctgctgag ttactaatct cgtcaccatc
      901 gactgcttgt aagatgtcgc caggttttat acccgcttga tcggctggac catctttgag
      961 cacacctgta atgaggatac cccctagctt ttttaatttg agggactcaa ccaactcagg
     1021 tgtaacatcg tgcatactta caccaagcca ccctcgcgtc acgctcccag tttcgatgat
     1081 ttgcttcatg atttgtatgg ctgcgctaac agggatggcg aatccaatac ccagtgatcc
     1141 ccctgttcga gaccagattg ctgtattgat tccaattagg ttgcctgcag tatccgttaa
     1201 agcaccgcct gaattaccag ggttaatcgc agcatccgtt tgtatgaaat tttcaaacgt
     1261 attgatgcca acttgtgacc gaccttttgc acctataatt cccattgtta ccgtttgacc
     1321 aaccgcaaag ggattgccaa ttgctaatac aatatcgcct atttttgcag attccgagtt
     1381 gccgatggta atacttggta ggttttctag atcaattttt aagacggcta aatcactttc
     1441 cggatcagag ccaataattt ttgccttagc atttcgacca tctaccagcg cgatttgtat
     1501 ttcgcttgca gcttctacaa catgatgatt tgtcaaaata tagccttcag cgctgacaat
     1561 aacaccagaa ccaaggctgg atgtttgttt cgttttgggg ctaaagcgat caccaaagaa
     1621 aaactgaaat gcttgatcat ccagttgagg atgagtctgc gatcgaactt ctttactagt
     1681 aaagatatta acaaccgcag gcattgcagt ttcagcggca gcattgaaac tatcaggtat
     1741 gatagaggga ttcgcggaag tatattcttt aattgtggcg attttcccac gaggcgtcca
     1801 tggaagtagt tctggtcgca atgtggaaac aacaaaaaag atagccaaca aaatcgtgac
     1861 agtttgtgca aaaatcaacc agagtttatt catttgcaaa ttcataccta agacaataaa
     1921 gcgaatatta aatatataac tgacaggttg gaagtaaaca atgttgcaat atgatctaga
     1981 aaattatctt aatcaattac tggaaatttc gcgctttcgc gattattgcc caaatggact
     2041 ccaagtggaa gggcataagc aaataaatac tttgattagc ggcgttacgg cttctctgga
     2101 attgatagag atggctattg cttgcaatgc tgatgcaatt cttgtgcatc atggttattt
     2161 ctggaaaggg gaagaatctt gcatacgagg tatgaaacat caacgcctca gtttgttact
     2221 gaaacataaa attaatcttt tcgcgtatca tttgccgctc gatgcacatt tggagttggg
     2281 gaataatatt cagttagcac gaaaactagg tttgcagtac gatggtactt ttggggaaca
     2341 agcaattgcg atgcatgggc aattggctaa tcgtatgatg cttggtgatt ttgggaagac
     2401 tattgcgact tctcttaatc gagaaccgtt gattattggt gatttatctc aaaaggtgca
     2461 gcgtattgca tggtgtaccg gtgcagcaca aggttatttt gaggaagcga ttgacgctcg
     2521 ggttgatgct ttcattactg gagaaatttc cgaacaaaac gtccatatgg ctagagaatc
     2581 aggggtcgcg tttattgctg cagggcacca tgcaacggaa cgttatggcg ttcaggcgct
     2641 tggtacgcac atcgcgcaac ggtttggagt caaccatgag tttatagatt taatgaatcc
     2701 agtatgacca gaatcgctag cgggcgctat gcagcatagg ggttctgatt agataaactg
     2761 gtagtgctca tcgtatcgga ctatttttaa ggcttatctt tatccttctt ttttttagga
     2821 atcgtccacc agataatgaa aagaaaaagc cccaacgcaa gtgctgcttc aaccactaat
     2881 acccacatat tattttcttg attgttttaa taaaatcgta ataacagcat accactaacg
     2941 tgaatttgat gaaaagatac tcgtttttta gtttattact attactcgcg cttagcgcat
     3001 gtaaaaccga atcgctttca acaaccaagc aagcagatac gatacaacca gctccaagtg
     3061 ttactgagaa tattaatcaa cttaggttaa cacaatggac acagttaatc ggttgggaag
     3121 cggatgagct attgcctgct tggaatacat tcatgcaaag ctgcactaca ttaaagcgcc
     3181 aacctaactg gcaagcagtt tgtacaaaag ccagtatgct tgagcaagca aatgagcaaa
     3241 ccatacgatt attctttgaa agtcaacttg caccgtatca ggtatttaat tcagacggca
     3301 gtgatactgg gattatcaca ggctattatg aacccttgct gcaaggtagt aaaaaatcaa
     3361 ctgagaaata tcgtttccca atttattcac ctcccgatga tctactcgtc attgatctcg
     3421 gtaatgcact tcccgatttt aagaatttac agcttcgagg acgccttgaa ggtcgtaagg
     3481 ttgttccttt ctatagtcgt gcagaaattg aaaaggggat tccaacgcta aaaaatcgag
     3541 aattactttg ggtagacgat cgggtagcgc ttttttttct ccatattcag ggttcagggc
     3601 gtatacagct tgagaatgga gaaattatta aggtaggtta tgcggatcag aatggccatc
     3661 cctatgtttc gattgggtca cttttagttc agcgcggtga attaccatta gaaaaagcat
     3721 cgatgcaagg tattaaacaa tggggtgtac agaatccaga aaaattaact gagttattac
     3781 atcagaattc ccgttatgtt ttttttcgat tacttcctga ttcattaacg ggcccactag
     3841 gttcactggg tgttccattg acggcgggtc ggagtcttgc catagatccc cgagttgtac
     3901 ctcagggcgc gcctgttttt ctttcgacga cctggcctaa tagtgataga tcattaaacc
     3961 gactaatggt tgctcaagat accggtagcg ccatcaaagg gggcgtacgc gccgattttt
     4021 tctggggatt tggtaaagaa gccgagaatc aagcaggaaa aatgaagcag aatggtaaaa
     4081 tgtgggtatt actaccgaaa gatatgacca ttccacctca ttcgaaataa ctcaagattc
     4141 tcaagtggtc atgccattat ggcggagcaa agcgtcaatt ttaggttccc gtcctcgaaa
     4201 tgcgataaat gactctagtg caggccgact tcctcccacc gctagaattt cgctccagaa
     4261 gcgactacca gcttgagtgt tgacgatatt acttggatcg gtttcttcaa ataaactgta
     4321 cgcatctgct gataagacct cagcccattt gtagctataa tatccggcag cgtatccacc
     4381 tgcaaaaata tgcgcaaaac tgttgggaaa acgactaaac gcgggtggaa taacaactgc
     4441 aacctgctga cgcacttcat cgagtaatgt taatacggtc ttttcgccat taggatcgaa
     4501 atcatagtgt agatgcatat cgaacagcgc aaattcaatc tggcgtaatg tttgtagacc
     4561 actctggaaa tttttggcag ccagtaattt gtcaaataaa gcacgtggga gcggtactcc
     4621 actttcgata tgagcggtca tttgactcaa tacgtcccat tcccagcaaa aattttccat
     4681 aaactggctg ggcaactcaa cggcatccca ttcaacccca ttgatgccgg acactgctaa
     4741 atcttcaact tgagtcagta aatggtgtag gccatgacca aattcatgaa atagtacgat
     4801 cacttcatcg tgggtaaaca atgcgggccg tgcctggcca tttttattga cgggtgccga
     4861 gaaattgcaa gtcaaataag cgaccggaat ctgtatagat gattgcctat tttcttgctc
     4921 gatgcgacgc cgagtcatcg catcatccat ccatgctcca ccgcgtttac tggggcgtgc
     4981 gtagagatca aggtaaaatt gaccgatcaa ttgacccttt gcatcatgaa tatcaaagaa
     5041 tttcacatcg ggatgccagc gctgaatggg gcgtgaaagg cttgcttcag aaatggtaat
     5101 tccatacagt gtttcaacta atcgaaacat tccagggagg actttgtttt cagggaaata
     5161 ttgtttaact tcttgatctg agaaggcata gcgttctaca cgaagttttt cacttgcata
     5221 tgctaaatcc catgattcta gcttttctaa cccgagtttg gtgcgggcaa atactttaag
     5281 ctcttctaga tctcgttgag cgtaaggctt tgctttagca gcaagttcat gtaagaaatc
     5341 gagtacttgt cgaggtgact cagccatttt gggcgctaaa gatacttctg cataacaatc
     5401 gaagccgagt aaattagctt cttctttgcg cagctttaga attttgttga tgagtggcat
     5461 attgtcccaa ttcgatgtgc catgcactag gctatcgatt tcgcttgcgc gagtttggta
     5521 ggcgcgatac atcttttcgc gtaagtcgcg attatcagca tattgcatga ccggtagata
     5581 cgaaggggca tgtaaagtaa acttccaacc cttggatggg ttaggtgctt tttcagcagc
     5641 ttctttggct gctaaaagcg catcttctgg gataccagaa agctcatctt gctgagcaat
     5701 aatgaggtag aaatcattgg tcgcatcaag gagattgtcg ctaaacttcg cagataaagc
     5761 agataactct tcttgaatcg caagaaagcg ttttttgtta tcggtagtca attctgcgcc
     5821 acccaatcta aaatcgcgta actgattttc aataattttc ttgcgagcat tgcttagatc
     5881 attaaattca gggctattgc gtaattgctt aaatttttca aataacgatt ggtcttgtga
     5941 taattccgca aaatactgcg ttactttggg taagttggca ttgtagactt cacgcagttc
     6001 tgggctattc attactgaat ttaaatgtga tacctgtccc caagctctgg atagtcgttc
     6061 gtttgcatct acgaggggtt gaataaaact attccaagta gcagtcgctg cggtgcgagc
     6121 ttgatttgca acggatcgac attgctgtag taatgtttca atagcaggca aaacatgttc
     6181 gggtttaata tccatgaaac ggggtaggcc agtaaaatca agtaagggat tcatagcaag
     6241 tgcacttaaa gaaaatgatt gaaagaaatg attgagtcga aaccgaccgg gtagtttagt
     6301 tactacgctg ataaatgata ttaccaccga ctaaagtata tttaacagaa ccaggaagct
     6361 ccatgcccat aaaaggagtg tttttacctt ggctctttag ggttgatggc gttactttcc
     6421 agtaggcttc agggtcaaag atacaaatat cggctgctgc acccaggctc aagtgcccag
     6481 catcaattgc caagatgcta gctggtgtct gggtaatgac ttttagtcca tcaattaatg
     6541 agagattcat tgaatcggtc cattttagcg tcaaagatag tagcaactct aatccactag
     6601 cacctgcttc cgattcaccg aaaggcagca atttagcgtc atcatcaatg ggtgcatggt
     6661 cggaacatat tgcatcgatc gtgccatcga tcaaacctgc tctcaacgaa tctcgatcct
     6721 gcaagtttct gagtggcggt ataaggtggc aattggtatt aaagaaacca atatccattt
     6781 cagaaagatg cagatgattg atagagacat cgcatgtaat cggtaatttt tgttgctttg
     6841 cagtgcgaag cattgccacg ctttcagcgc ttgagatacg gcacacatga acacgaacac
     6901 caatttcacg gaccaataaa ataatattag ataaggcaat ggtttcagca caaactggaa
     6961 taggggataa cccgaggcgt gtggcgactt caccatcatg tgcaacccct ctattcgcta
     7021 ggttaatttc ttgagggcgg agccaaacac taaagttgaa ggttgatgcg taatacattg
     7081 cctggcgcaa aatatggagg tttgttattg gctcatctgc gtgactgaat gcaacacacc
     7141 ctgcatccct tagctcggcc atttctgtga gtttctcgcc ttttaatttt tgggttagcg
     7201 ccccgatagg ataaacatgg atatgattca agcttttagt gcgatgtttt aacatttcaa
     7261 ccaaacccgg ttcatccagt ggagggtcgg tatcaggggg gcaggcaagg ctagtgatac
     7321 ctccagctgc agcagcatca agctcggatt ccaaggttgc tttatattca agtcctggct
     7381 ctcgtaaacg cgcagacaaa tcaattaacc caggacaaac cacccaatct ttggcatcga
     7441 taacggggca atccgtaaat cctgtgggaa tgctaccaat tgcaacgatt ttgccctcag
     7501 cgatacagag atctttaatt gcatcgagtt tattttgggg atcgatcaaa cgtccat
//
LOCUS       DAOQKQ010000131         7364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_4959_length_7364_cov_5.606649, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000131 DAOQKQ010000000
VERSION     DAOQKQ010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 7364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7364
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4959_length_7364_cov_5.606649"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1704)
                     /locus_tag="PKZ49_09040"
     CDS             complement(<1..1704)
                     /locus_tag="PKZ49_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36746.1"
                     /translation="MTVEQVSSSLQSMNTQIPVALSPQSPWPGLAAYEEEAHDYFYGR
                     KEEAAELLRMIRLSGLTMLYGKSGLGKTSLLQAGLYPLLRAEHFFPIHLRIDFRPSAP
                     YPPLEQVLQKLLGALTGANADFPTPNENEGLWRYLHRRDLEIWSSDNYLLKPVLVFDQ
                     FEEIFSQGEHYPEQTQLTLNALADLIENRIPQELTIGDKHRQTLSALDLQAQRYRIVL
                     SFREDFLPEIENWKEQAPSLLRNRLRLLPMFRERAIEAVKAAGAEVLAEGVAEPLVDF
                     VGNLEASKLKSLPIIEPVLLSLCCYQLNQRRLPNHKIDIELLQQAGQDILQDFYHEAL
                     AGMPETVSEFIETHLIQGSRYRSSYPVDQALQDKFLTEEQLTRLTSQHRLLRIDQQLG
                     INRIELIHDRLVGVVSQVRDERLKKLEIEKIRAKEKERQRQLALSTALEKQAAAEDLA
                     AERLKATKRAQADAIRLRKQARQLQWALLSVLVLAILAGYGFYNTQTEKKERKLAQRE
                     SISLRLVSESLDITNGLRPGGDERALLQLLAAKQITPNHRKVDAALIDLMNKRVMTDK
                     IW"
     gene            complement(1708..3078)
                     /locus_tag="PKZ49_09045"
     CDS             complement(1708..3078)
                     /locus_tag="PKZ49_09045"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HND36747.1"
                     /translation="MNERNWDRLLKSIEEGLVVPVMGPQLITSLNGSSSFQSQIAQQL
                     LKMYDYALGHDVLMPHQELNDAVTRLKRDHPIQDLYGDIHDVIGQLTPNTDAELPEAI
                     KQITAIRDFRLFVTLTPDDLLARSLQAQVTVNEIIHSPYLPTSEGSDLPNDWRSRPGE
                     VYLLYLFGKSRSAPMFAIHDEDMLEYVHNVIARGSHVPVKFFAELQQRNLLLIGCNFP
                     EWLSRFFLRLMNQKRLSDTQRKREWLIDALQPEKELIYFLESYSEGTEVLSDISPQFF
                     IQQLSQRWLARHGADEQAPLSQVEMVPKHTLFFISYCRAPDLPAAIQLVAALKTLGVA
                     EHEIWFDKSAIEPGQDFRERILDGIRTCRYFIPLISSAADQLDEKFFRREWNEAIDRS
                     KSIQGRIFIVPIIADQNYDPEGYIRVPLAWKDNFHFGHAPLGQPNEQTNALLKKLIRS
                     ERQQSG"
     gene            3308..6034
                     /locus_tag="PKZ49_09050"
     CDS             3308..6034
                     /locus_tag="PKZ49_09050"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HND36748.1"
                     /translation="MAHDSTPNPQLAIILEAIQNRDYPDSIEQIKAYVAMLETEERFY
                     PARQLLSQAREKYRSIRQFQSEPTAVNWWQTDAAQDEVWITQKQAFFTYKNPELPPRT
                     RFIDSLLLLDEIGLRDSTNRDAETLALGGSIYKRKWQHFGQLEDLIEALFFYRAAFER
                     NPQQDKGYGGVNAALILDILADRAKQIARRSGIKLNESEASNFSAQAAAIRQNMVLQI
                     PVWQTELKQLPESDHFWPQVTFAEIYFGLQQYDQTKEWLQKANVAHAINWKKQTIYSQ
                     LLQIGRLQDLTIPKESDQPENWLPAWQILALIIGEDNAQRAFSSGTGKVGLSLSGGGF
                     RASFFHLGVMARLAEIDALRSIEALSTVSGGSIVGAHYYLEVQKLLESKPDKAITRND
                     YIEIVRRIQDNFLKGVQTNIKLQTFASLTDNLDVFFRKKHYSRSHRLGELYESEIYQS
                     VDDGHTNQIPRIMPELLIKPEGEADTQTHKPKHYNWLRRAKIPALVINSTSLNSGHNW
                     QFTARSMGEPPSQIDGEIDNNTRYRRMYYEHTPNETLQKYRLGYAVAASACVPGLFEP
                     LTIADLYQDKIVRLVDGGVHDNQGVAGLLDEGCTRILCSDACGQMDDVDQPSDSRPGV
                     VLRASSILQDRVREAEYQDLSNRLDSHALDGLFFVHTKKELESQPLDWINCQDPRTPA
                     HLQDPKTSYGIDRELQKKIAGIRTDLDAFTEVEAYAIMASGYRIAKREFELLQNQYQK
                     EGHTDHWGGYDIDAPETDWPFRKLEPIMGLKAEANKQSQDLHFQLHVARKIFFKTYDL
                     RLDYRIIVWSLLALLIAGILSFLYQQWNTPLTDTLHLGSLTWGKLFSLLILMVISALI
                     PMVHLLSPSNKVANIFKFMVAAGGSLLAKFHLAVLDRWFLKRGKLERLLKLK"
     gene            6078..6872
                     /locus_tag="PKZ49_09055"
     CDS             6078..6872
                     /locus_tag="PKZ49_09055"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HND36749.1"
                     /translation="MTRYKEGDSGKDIKRIQTALKDKGFNPGALDGEFGPGTEAAVIA
                     FQKSEGLVADGVIGTQTLKALGLIESDQPESFDLQGVTSINVSKMFPQTPISHIKTHL
                     PFVLDALKDAGLADRQMILMALATIRAETASFEPISEGKSKYNTSPNGHSFDLYDNRK
                     DLGNRGKPDGDRYKGRGFVQLTGRANYTKFGKEISQDLINNPDLANDPGIAAQLLARF
                     LKKGERKIRACLDDHDLAGARKVVNGGKHGLVEFSEAYNIGNKLFA"
     gene            6906..>7364
                     /locus_tag="PKZ49_09060"
     CDS             6906..>7364
                     /locus_tag="PKZ49_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36750.1"
                     /translation="MLKIKSILVLALLGISQSVVSANTHCSHQEVIVFNCNIGKKVVS
                     ICASPNLSAKTGYLQYHFGPIHSPELVFPSKKIAPHDQVTGGTLTFSGGGGAYLRFIR
                     NHHRYVVYTAIGRGWGEKAGVNVEKNGHRKANLICRNQPISELGPDFFERA"
BASE COUNT         2132 a         1670 c         1564 g         1998 t
ORIGIN      
        1 ccatatctta tccgtcatta ctcttttatt catgagatca atcagtgctg catcgacctt
       61 gcgatgattc ggcgtaatct gctttgctgc taacagttgc aacagcgccc gctcatcgcc
      121 acccggacgc agtccattgg taatatcaag gctctccgat accaaccgaa ggctgattga
      181 ttctcgttgc gccagttttc gttctttttt ctcagtctgc gtgttataaa aaccatagcc
      241 agccaaaata gccagtacta aaacactcaa caaagcccat tgtaattgtc ttgcttgttt
      301 ccgaagacga attgcatccg cctgcgcacg tttggttgct tttaaccgct ctgcagccaa
      361 atcttctgca gcagcttgtt tctcaagcgc agtagataac gccagttggc gttgccgttc
      421 tttttctttt gcgcgaatct tctcgatctc aagtttcttt aaacgctcat cccttacctg
      481 gctcacaaca cccactaatc gatcatgaat gagttctatg cgattaatcc ctaattgttg
      541 atcaatgcgt agtaaacgat gttgactagt cagtcgagtc aattgttctt ctgttaaaaa
      601 tttatcttgt aacgcttgat caactggata gctacttcga taccggctcc cttgtatcaa
      661 atgggtttca atgaattcgg atactgtttc tggcatacct gccagcgctt cgtggtaaaa
      721 atcctgcaga atatcctgcc ccgcttgttg taataactca atatcaattt tatggtttgg
      781 taaacgtcgc tgattgagct gataacaaca caagcttagt aaaacgggtt caataatcgg
      841 taatgatttc agcttagatg cctcgagatt ccctacaaaa tcaacaagcg gttcagctac
      901 accttcggct agcacttcgg caccggcagc tttaaccgct tcaatggcac gttcccgaaa
      961 catcggtaat aaacgtaagc gattacggag caatgaaggc gcttgctcct tccaattctc
     1021 aatctcagga agaaaatctt cacgaaatga aagtacgatt cgatagcgtt gtgcctgtaa
     1081 atccaatgct gatagggttt gccgatgctt atcgcctatt gtcaattctt gtggaattcg
     1141 attctcaatc aggtcagcta atgcgttgag tgttaattgt gtttgttcgg ggtaatgctc
     1201 accttgcgaa aaaatttctt caaattgatc aaaaacgaga acgggtttta gcaaataatt
     1261 atcgctactc caaatttcaa gatctcgccg atggagataa cgccataatc cctcgttttc
     1321 atttggagtt ggaaaatcgg cgtttgcccc cgttaatgca cctaataact tctgcaatac
     1381 ttgctctaga ggtggataag gtgcagaagg tctgaaatcg atgcgtaaat gaatcgggaa
     1441 aaaatgttct gcacgtaaca gtggataaag tccagcttgt aacaatgacg tttttcctaa
     1501 acctgactta ccataaagca tggtcaatcc agataagcga atcatgcgca ataactcggc
     1561 tgcttcctcc ttgcgcccgt aaaaataatc gtgcgcctct tcctcatacg ctgccaatcc
     1621 tggccatggc gattggggtg acaaagccac tggtatctgg gtattcatcg attgtaaaga
     1681 tgatgaaact tgctctacag tcataaccta gccactctgt tgtcgttccg atcgaattaa
     1741 ttttttgagt agcgcattgg tttgctcatt gggttgaccc agtggtgcat ggccgaaatg
     1801 aaaattatcc ttccaggcca atggaacccg aatgtaacct tctggatcgt aattctgatc
     1861 agcgataatg ggaacgataa agatacgccc ttgaattgat ttgcttcgat cgattgcttc
     1921 attccattca cgccggaaga atttctcatc taattgatca gcagcgctgg aaattagcgg
     1981 aataaaatag cggcaagtac ggataccgtc tagaattcgc tcccggaaat cttgacccgg
     2041 ttcgatcgcc gatttatcga accatatttc atgttctgca acacccaatg ttttcagtgc
     2101 cgctaccagt tgtattgctg caggcaaatc cggcgcacgg caatagctaa tgaaaaagag
     2161 tgtatgtttg ggaaccattt caacttggga taatggcgct tgctcatctg caccatgacg
     2221 cgctaaccag cgctgagaaa gttgctggat aaaaaattgc ggagagatgt cggatagcac
     2281 ttcggttcct tcactatagc tttctaaaaa atagattagc tctttttctg gctgtagtgc
     2341 atcgatcaac cattcacgtt tgcgctgcgt atctgacaat cgtttttgat tcatcaatcg
     2401 caagaaaaaa cgactcagcc actctggaaa attgcagccg atcaatagca aattgcgttg
     2461 ttgtaattct gcaaaaaatt taaccgggac atgacttccc cttgcgatca cattatgcac
     2521 atattccagc atatcctcat catgaatcgc aaacattggc gcagatcttg atttaccaaa
     2581 caaatacaat agataaactt ctcccggtcg agaacgccaa tcattaggca aatcagagcc
     2641 ctcgctggtc ggtaaataag gtgaatgaat aatttcattg acagtcactt gcgcttgtaa
     2701 gctgcgtgct agtaaatcat cgggtgtcaa agttacgaac aatcgaaaat cacgaattgc
     2761 agttatttgt ttgattgctt cgggaagctc tgcatcagta ttaggcgtta attgtccaat
     2821 gacatcatga atatcaccat aaagatcctg aatagggtga tcgcgcttca agcgcgtcac
     2881 agcatcgttc aactcttggt gtggcattag cacatcatga cctaacgcat agtcatacat
     2941 tttaagtaac tgttgggcaa tctgcgactg aaacgaggat gagccgttaa gagaagtgat
     3001 cagttgtgga cccattacag gcacaactag tccctcttct atcgatttca gcaaacgatc
     3061 ccaattacgc tcattcatgg tttaacaatc tttaccttaa caatccggat tttatgacgg
     3121 aaatcgtcgt tgtggttagt ttttagcgca gaataggcct tgtgattttc ttcacgtcaa
     3181 atcaccgtaa aatacggtag catagtagaa caagaacaca acatatcatc tgcgtactcc
     3241 attatcaaag aacaacagta gcagatgctt ttgattgaac gcatgttatc gaacaaggag
     3301 cttgctcatg gcccacgatt caaccccaaa ccctcaatta gcaatcattc tagaagcgat
     3361 ccaaaatcgc gattatccag actctatcga gcaaattaaa gcctatgttg cgatgctaga
     3421 aacggaagaa cgtttctatc cggctcgaca attactcagc caagcacgcg agaaatatcg
     3481 ctcgattcga caattccaat cagaacccac cgcagtaaac tggtggcaaa ccgatgctgc
     3541 gcaggatgag gtttggatta cccaaaagca agctttcttt acgtataaaa atcctgaact
     3601 gccacctcgt acacgattta tcgattcact gttattgctt gacgaaatcg ggttgcgcga
     3661 ctcgaccaat cgtgacgcag aaacgctagc cttagggggg agtatctata aaagaaagtg
     3721 gcagcatttt ggtcaattag aagatctcat cgaagcatta ttcttttatc gcgccgcatt
     3781 tgaacgtaac cctcaacagg ataagggcta tggtggcgtc aatgctgcgc tgattctcga
     3841 tatcttggct gatcgcgcta agcaaattgc acgccgcagt ggcattaaac tcaatgaaag
     3901 cgaagccagc aatttttctg cacaagctgc tgcgatacgc caaaatatgg tactacagat
     3961 tccggtttgg caaactgaac tcaagcaatt accagagtca gaccatttct ggccacaagt
     4021 cacattcgca gaaatttatt ttggcttgca gcaatatgat caaaccaagg aatggttgca
     4081 aaaagccaat gtagctcatg caatcaattg gaaaaaacaa accatataca gccagttgct
     4141 gcaaatcggg cgcctacaag acttgacgat tccgaaagaa agcgatcagc ctgaaaattg
     4201 gctaccagct tggcaaatac tcgcgttaat cattggtgaa gataacgctc agcgtgcctt
     4261 ttcatcagga actggtaaag tcggattatc gttatcgggc ggtggtttcc gagcttcatt
     4321 tttccacctg ggcgtcatgg ctaggcttgc tgaaatcgat gcattacgca gtatcgaagc
     4381 attatcgaca gtatcgggcg gcagcatcgt cggtgctcat tattacttgg aagtacaaaa
     4441 attgcttgaa agcaaaccag acaaagcaat tacacgtaat gattacatcg aaatcgtgcg
     4501 acgcatccaa gacaatttcc tgaaaggcgt acaaaccaat atcaaactgc aaacatttgc
     4561 aagtttgact gataatctcg atgtattctt ccgcaaaaaa cattattctc gcagccaccg
     4621 cctcggggaa ttatatgagt cagaaattta tcaatcggtt gacgatgggc atacgaacca
     4681 gataccgcgc atcatgcctg aattgcttat caaacccgaa ggggaagcgg atactcagac
     4741 gcataaacca aagcactata attggctacg ccgagccaaa atcccagcgc tcgtgatcaa
     4801 tagtacttca ttgaactccg gacataattg gcagtttacc gcgcgttcca tgggcgagcc
     4861 acccagccaa atcgatggtg aaattgataa caatacacgc tatcgccgta tgtactatga
     4921 acatacccct aacgaaaccc tacaaaaata tcgcttgggc tacgccgtgg cagcctcagc
     4981 ttgcgttccc ggtttatttg agccattaac aatcgctgat ctgtatcaag ataaaatcgt
     5041 gcgcctagtc gatggtggcg tacatgataa ccaaggagtt gcaggcttgc tcgatgaagg
     5101 ttgtacccgt attctgtgca gcgatgcctg tggccaaatg gatgacgtcg atcagccttc
     5161 cgattcacgt cctggtgtcg tactccgcgc tagctcaatc ttacaggatc gagtacggga
     5221 agcggaatac caagatctta gcaaccgctt ggatagtcat gcgctcgatg gcttattttt
     5281 tgtgcacacc aaaaaagagc ttgaatccca accattggat tggatcaatt gccaagatcc
     5341 tcgtacgcct gctcatctgc aagaccctaa aacatcgtat ggcatcgatc gggaactaca
     5401 aaagaagatt gcagggatcc gcaccgatct tgatgcattt accgaagttg aagcttatgc
     5461 catcatggca agcggttatc gcattgctaa acgggaattt gagctgttac aaaatcaata
     5521 ccaaaaagaa ggtcataccg atcattgggg cggatacgat atcgatgcac cggaaaccga
     5581 ttggcctttc cgtaaactag aacccatcat ggggctcaaa gctgaagcta acaaacaaag
     5641 tcaagattta cattttcagc tccacgttgc acgcaaaata ttctttaaaa cctacgattt
     5701 gcgactggat tatcgcatca tcgtttggtc attattagcg ctgctaatag caggtatact
     5761 cagtttcctt tatcaacaat ggaacacgcc actgactgac acgctccatt taggatcgct
     5821 cacgtgggga aaattgttca gtctattgat attaatggtc attagtgcat tgataccaat
     5881 ggttcatttg ctaagtccaa gcaataaagt agctaatatt ttcaaattta tggttgcagc
     5941 tggcggtagt ttattagcaa aatttcatct ggcagtgctc gatcgttggt tcttgaaacg
     6001 cggcaaactt gagcgattgt tgaaattaaa ataacgatga aacatattgt acgacctaac
     6061 aacagagggg aaaaaccatg acccgctaca aagaaggcga ttctggtaag gatattaaac
     6121 gcatccaaac agcgctaaaa gataaggggt tcaatccggg tgcactcgat ggtgaattcg
     6181 gtccaggtac agaggctgcc gtcatcgctt tccagaaaag tgaaggtcta gtggcggatg
     6241 gggtaattgg tacccaaacc ctgaaagcgc tgggtctaat tgaatcagat cagcctgaat
     6301 cctttgatct acaaggtgtt acttcgatta atgtatcgaa aatgtttcca caaaccccca
     6361 tcagtcatat caaaacccac cttccttttg tgcttgatgc cctaaaagat gcgggcttag
     6421 cggataggca aatgatactc atggctcttg ctacgattcg tgctgagact gcaagtttcg
     6481 aaccgattag cgagggcaaa tcgaaataca acacatcgcc caatggtcac tcatttgatt
     6541 tatacgataa ccgcaaagat ttaggcaatc gaggtaaacc tgatggagat cgctataaag
     6601 gacggggatt tgtacaatta accgggcgag ctaattatac aaaattcggc aaagaaatca
     6661 gccaagatct aatcaataat cctgacttag cgaatgatcc cggtattgca gcgcaactgt
     6721 tagcccgctt tctgaaaaag ggtgaacgta aaattagggc atgcttggat gaccatgatt
     6781 tagctggagc gaggaaagtc gtgaatggcg ggaaacatgg attagttgaa ttttcagagg
     6841 cttacaacat aggaaacaag ctttttgcct gaataattcg gagacattaa acaagtgctt
     6901 ttgccgtgct gaaaatcaag tcgattctag ttttagcttt acttggaatc agtcaatcag
     6961 ttgtttctgc caatacacac tgcagtcacc aggaagtaat tgttttcaat tgcaatattg
     7021 gtaaaaaagt cgtatcaatt tgtgcttccc caaacctcag tgcaaaaact ggctatctgc
     7081 aatatcattt cggccccatt cattcccctg agcttgtttt tccaagtaaa aaaattgcac
     7141 cccacgatca agtgacaggg ggtactttga ctttttcagg aggtggtggt gcttatcttc
     7201 ggtttatccg caatcaccat cgttatgttg tttataccgc aattggccgt ggttggggag
     7261 aaaaagctgg agtcaacgtt gaaaaaaatg gtcatcggaa agcaaatcta atctgtcgta
     7321 atcagcccat ctcagaactt ggccctgatt tctttgaacg tgca
//
LOCUS       DAOQKQ010000132         7316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_5008_length_7316_cov_5.276408, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000132 DAOQKQ010000000
VERSION     DAOQKQ010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 7316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7316
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5008_length_7316_cov_5.276408"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..549
                     /gene="trxA"
                     /locus_tag="PKZ49_09065"
     CDS             223..549
                     /gene="trxA"
                     /locus_tag="PKZ49_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HND36751.1"
                     /translation="MAQHIHYITDATFEAEVLQSPTPVLVDYWAEWCGPCKMIAPILD
                     EVANEYNDRLKVAKLNIDENQHIPQKYGIRGIPTLMLFKNGNIEATKVGALSKSQLTA
                     FIDSHL"
     gene            678..1937
                     /gene="rho"
                     /locus_tag="PKZ49_09070"
     CDS             678..1937
                     /gene="rho"
                     /locus_tag="PKZ49_09070"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HND36752.1"
                     /translation="MRLSDLKSLHVSELIKMAVANEVEGANRLRKQDLIFALLKNQAR
                     KNESIFGEGTLEILQDGFGFLRSPDTSYLAGPDDIYISPSQIRRFNLHTGDSVDGEIR
                     PPKDGERYFALVKVDKVNNEPPENSKRKILFENLTPLFPTERLVLERDIRAEENTTGR
                     IIDLIAPIGKGQRGLLVASPKSGKTVMLQHIAHSIAANHPDVILMVLLIDERPEEVTE
                     MIRSVRGEVISSTFDESAARHVQVADMVIEKAKRLVEHKKDVVILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIATALVDTGSRMDDVIYEE
                     FKGTGNMEIHLDRRMAEKRIYPAINVNRSGTRREELLLPQEILQKIWILRKLLYPMDD
                     MDAMSFLLDKIKATKNNADFFDSMRRV"
     gene            complement(2016..2486)
                     /locus_tag="PKZ49_09075"
     CDS             complement(2016..2486)
                     /locus_tag="PKZ49_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HND36753.1"
                     /translation="MGFCVFLGLMTWYLGKPPSIRFYETKSEAPLTAPLAPGINMALD
                     MQSSVAVRLSEPLHVELFKGNVYFDIQKQATHQLEVKVGSTIIKDFGTRFSIQLHKDG
                     TGHIAVTDGHIKIHSPSGNVFQINAYEEAEFDETGISKHRLLSEKNIAPWHILR"
     gene            2927..3976
                     /locus_tag="PKZ49_09080"
     CDS             2927..3976
                     /locus_tag="PKZ49_09080"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HND36754.1"
                     /translation="MTDIVNLLGQEADQLLAHVCQGIPKANLQIPGPDFIDRVLLNSD
                     RKPGVLRNLQALYNHGRLAGTGYLSLLPVDQGVEHSGGASFAPNPLFFDPENIVRLAI
                     EGGCNGVASTLGVLGMVARRYAHKIPFILKINHNELLTYPNKFDQTLFASVDQAFEMG
                     ACAVGATVFFGSEESRRQIQEISEAFAHAHELGMVTILWAYTRNTAFKTPEKDYHVSA
                     DLTGQANHLAVTLEADIVKQKMAENNGGYTALKFGKTNAKVYSELTSDHPIDLARYQV
                     ANCYMGRVGMINSGGESGKDDLHQAVRTAVINKRAGGMGLISGRKAFQKPFDEGVKLL
                     NAIQDVYLSQQITVA"
     gene            complement(4046..4894)
                     /gene="rpoH"
                     /locus_tag="PKZ49_09085"
     CDS             complement(4046..4894)
                     /gene="rpoH"
                     /locus_tag="PKZ49_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HND36755.1"
                     /translation="MTDICALPSVVGGNIESYIQAVNRCPILSQEEETGLARSLRNDG
                     NIEAAHKLVLSHLRVVVAVARGYLGYGLPHADLIQEGNVGLMKAVKRFDPERGVRLIS
                     FAIHWIKAEIHEFIMRNWRLVKIATTKQQRKLFFNLRSMKQSLDTMSQAEVNTVAKQL
                     GVKPEEVVEMETRFSGRDVSLEPSGDDEDDITSPIHYLTDGSDPAQQVEQEQIARLYH
                     DKLQESLASLDDRSRHIIEARWLREQDTATLHELADELGVSAERIRQIEAKAIQKIKS
                     TVGAVL"
     gene            complement(5130..6371)
                     /gene="glcF"
                     /locus_tag="PKZ49_09090"
     CDS             complement(5130..6371)
                     /gene="glcF"
                     /locus_tag="PKZ49_09090"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HND36756.1"
                     /translation="MQTNLADFVKKNQNSTEAEAILRSCVHCGFCLATCPTYQLLGDE
                     LDSPRGRIYLMKQVLEGQAVTQKTQLHLDRCLTCRACETTCPSGVRYSHLLDISRGIV
                     EEKVARNRYSELKRFAIRKFLTSRSIFKFSVRIAQLLRAFMPAKLKAAIPPLQRSAVN
                     WNNSRHPRKMLVLDGCVQPTLAPNINAATARVLDKLGISLIKVEEAGCCGALAYHLNA
                     QQQGLDAMRHNIDVWWPLIETHQIEAIVISASGCGVTVKEYGHFLQYDAAYAEKAQVV
                     AALAKDISEIITAELENLTVLLKETSLNHPGKLAFHSPCTLQHGMKIRGVVEKILSSA
                     GITLCQVDNPHLCCGSAGTYSILQPTLSQQLLKDKVIALSANGPAQIVTANIGCLMHI
                     QSGTTLPVKHWIELLDTLIHT"
     gene            complement(6375..>7316)
                     /gene="glcE"
                     /locus_tag="PKZ49_09095"
     CDS             complement(6375..>7316)
                     /gene="glcE"
                     /locus_tag="PKZ49_09095"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HND36757.1"
                     /translation="LDVTPYSGIVDYDPSELVITAKAGTRLVELESLLLQSNQMLAFE
                     PPHFTEHTTLGGTIAAGLSGPRRAYQGSVSDFVLGIRMLDGQGNDLRFGGRVMKNVAG
                     YDVSRLMVGSLGTLGVILEASLKVLPRPETELSICLEMNQSQAIEKIHQWASKPLPIS
                     ASCYHENKLLIRLSGSPAGVSTACQKIGGELLESSDSFWRSLRNHTHPFFTSGKRLWR
                     LSVKSTLLPIALEGEQLIEWGGALRWLATDGQENDQTLRQQIGDLNGHATLFYGDKSN
                     IQAFHPLSPSMLKIHQKLKQQFDPLSIFNHGRMYSEF"
BASE COUNT         2009 a         1480 c         1657 g         2170 t
ORIGIN      
        1 gtaaatatat tgggatgtca tcgaatattt ttccgaatga tatgcattgc ttgcttccca
       61 ttttcttttt ttttatatac tatatccatt atactgatta cacatcccaa tttaatccca
      121 ttccagctta aaaagtctta atgacaatat aactaaactt aatttcgaaa ttaattaagt
      181 taatggttat caatatctga aatcgataca tacgaggtat tcatggctca acatattcat
      241 tatataactg atgcaacatt tgaagcggag gttttgcaat cacccacccc ggtgctagtc
      301 gattattggg ctgagtggtg tggcccttgt aaaatgattg cacctattct tgacgaggtg
      361 gccaacgaat ataatgaccg ccttaaagtc gctaagctaa atatcgatga gaatcaacac
      421 atcccccaga aatatgggat tcgtggaatt ccaacgttaa tgttatttaa aaacggcaat
      481 attgaagcga ctaaagtggg cgctttatcg aagtctcagc taactgcctt tattgatagc
      541 cacctttgag ataactaatc aagctatttg acaacaaatt aaagaatcaa tttgtattcc
      601 tttagtgatc agtcagcagg ttacaacatc taagtctctt caagtttttc ttttctgatc
      661 tttcaatcat attctccatg cgcttatctg atctaaaaag ccttcatgtt tccgaattaa
      721 ttaaaatggc agttgcaaat gaagtcgaag gagccaatcg cctgcggaag caagatttaa
      781 tttttgcctt attaaaaaac caagcacgta agaatgagag tatttttggt gaaggtactt
      841 tggaaatttt gcaagacggt tttggcttct tgcgctctcc tgatacttcg tacttagcag
      901 gtcctgatga tatttatatt tctccaagtc aaattcgacg tttcaacctg catactgggg
      961 attcggtaga tggagagata cgtcctccta aggatggtga gcgttatttt gctttagtga
     1021 aagttgataa agtcaataat gagccgcctg aaaattctaa acgcaagatt ctgtttgaga
     1081 atttaactcc tttattcccc actgagcgtt tagtactaga acgcgatatc cgggccgagg
     1141 agaatacgac tggaagaata atcgacctta ttgcaccgat cggtaaagga caaagagggt
     1201 tattggttgc tagtccaaaa tctggaaaga cagtgatgct gcaacatatt gcacactcga
     1261 ttgcagccaa tcaccctgat gttattttga tggttttgct gatagatgag cgccctgaag
     1321 aagtgaccga aatgattcgt tcagtaagag gtgaagttat ctcttccact tttgatgagt
     1381 ctgctgccag gcacgtacag gttgcagata tggtaataga gaaggccaaa cgactagttg
     1441 aacataagaa agatgtcgtg atattgcttg attcgattac tcgtttggcg cgtgcttaca
     1501 atacagtcgt ccctgcttca ggtaaggtac ttactggtgg ggttgatgct aatgctttgc
     1561 agcgccctaa gcgatttttt ggtgccgctc ggaatattga agaaggtggg tcattgacca
     1621 ttatcgccac tgcacttgtg gatacaggat ctcgtatgga tgatgtgata tacgaggaat
     1681 tcaaaggtac gggtaatatg gagattcatt tagatcgccg aatggctgag aagcgtattt
     1741 atccagccat taatgtcaat cgttctggaa cacgaagaga agaattattg ctgccacaag
     1801 agatacttca aaaaatatgg atcttgcgca aactgcttta tccaatggat gatatggatg
     1861 caatgtcatt cttgttggat aaaattaaag caactaaaaa taatgctgac ttttttgatt
     1921 caatgcgcag agtataattg aattaggcag ggttaatatt tagtactatt ttcttacccc
     1981 cccactagca aatctctgat tgtatattga ggggattacc gcaagatgtg ccaaggcgcg
     2041 atgttttttt cgctgagcaa gcggtgtttg ctaatgccag tttcgtcaaa ttcggcttcc
     2101 tcgtacgcat tgatctggaa aacatttcct gaagggctat ggatcttgat gtgcccgtcg
     2161 gtaactgcaa tgtggcccgt gccgtcctta tgtaattgaa tgctaaaacg ggtgccaaaa
     2221 tctttgatga tggtgctacc tacttttact tccaattgat gggtggcctg tttctgaata
     2281 tcaaaataaa catttccttt aaatagctcg acgtgtaacg gctcacttag tctcacagca
     2341 accgagctct gcatatctag cgccatattg atacccggtg ctagtggcgc cgttaaaggg
     2401 gcttccgatt tcgtttcgta aaagcggata ctcggcggtt tgcccaagta ccaagtcatc
     2461 aatcctagaa atacacaaaa gcccatgatc gcactatgca cgaaaaagcg cttccagcta
     2521 aaaacgaaag tataaggttc aacgaccggg ggtttggggt cctgcattgc ttcggtaaaa
     2581 aatgaaaaga agcattatat accgatgacg atcattgctt gcctaaatgt gatggatgag
     2641 ctgagtgatt gttgattggc tggctgtagg ccatgaagct cagcagtggg gccgcaacgc
     2701 aactgcggat ggagtaagct atagcttaaa ggggaagcaa tttgtgagtg ggaggagcgc
     2761 tatattcaag aacacgaaac atcgctgggt aaggcgcgat taactagtgg tggaatacgg
     2821 caaagatggt gtttgtcgtc gtggttttat cgatgttttg agttgctttg ttacctgatg
     2881 ctatttttgg gcgtatactc aaaaaaatgg tgataaggag taagcaatga ctgatattgt
     2941 taatttattg ggtcaagaag cagatcaatt attagcgcac gtttgtcaag gcattcctaa
     3001 agcaaatcta caaatacctg ggcctgattt catcgatcgg gtgctactga atagcgatcg
     3061 taaacctggc gtattgcgta acctgcaggc actttataac catggtcgct tggcgggaac
     3121 cggctatctt tcattactgc cagtggatca aggagttgaa cattccggcg gtgcttcgtt
     3181 tgcaccgaat ccattgtttt tcgaccctga aaatattgtt cgacttgcga tcgaaggggg
     3241 gtgtaatgga gtggcatcga ctttaggtgt attgggtatg gttgcgcggc gttatgcgca
     3301 taagattccg ttcattctga agatcaatca taacgagtta ttaacttacc cgaataaatt
     3361 cgatcagacg ttatttgcga gtgttgatca ggcttttgag atgggcgctt gcgcggtggg
     3421 cgccacggtt ttttttggct ctgaggaatc gcggcgccaa atacaggaaa tttcagaagc
     3481 ctttgcgcat gcgcatgagc taggaatggt gactattctt tgggcgtata cacgcaatac
     3541 cgccttcaag acacctgaaa aagattatca cgtgagcgcg gatttgacgg gccaagcgaa
     3601 ccatttagcg gtgacgctcg aagcagatat cgtcaagcaa aaaatggctg agaataatgg
     3661 gggctacaca gcgcttaaat ttggtaaaac caatgccaag gtttatagcg aattaactag
     3721 cgatcatccg attgatttgg ctcgatatca agtagccaat tgttatatgg gccgagttgg
     3781 catgattaat tcggggggtg aatccggcaa agacgatttg catcaagcgg tgcggacagc
     3841 ggtcatcaat aagcgagctg gcgggatggg gctgatttct ggtcggaagg cttttcaaaa
     3901 gccatttgat gaaggcgtta aattactcaa tgccattcag gatgtttacc tcagtcaaca
     3961 aattactgtg gcttagcgct gcattcgctt aattattgag ttagctgtgc ttcttgacct
     4021 gaagcacagc cattaaggta gattgttata gaaccgcccc aacggtactt ttgatttttt
     4081 gaattgcttt tgcttcaatt tggcgaatgc gctctgctga tacgcctaat tcatctgcaa
     4141 gttcatgcag cgttgcagta tcttgctctc ttaaccagcg cgcttcgata atatggcggc
     4201 tacgatcatc taaactggca agcgattctt gtaacttatc atgatataag cgggcgattt
     4261 gctcttgttc gacttgttga gcgggatcgg aaccatccgt taagtaatga attgggctag
     4321 tgatatcatc ttcatcgtca ccactcggtt ctagtgaaac atctcgaccg ctaaagcgag
     4381 tttccatttc gaccacttct tccggtttga cacctaattg tttagctacc gtgtttactt
     4441 cagcttgcga catcgtatcc aggctttgtt tcatactcct gagattgaag aaaagtttac
     4501 gctgttgctt cgttgtggcg attttaacca accgccaatt acgcatgatg aactcatgga
     4561 tttcagcttt tatccagtga atcgcaaatg aaattaatcg taccccgcgt tcgggatcaa
     4621 agcgttttac cgctttcatc aagccgacat ttccttcttg gataagatca gcatggggta
     4681 atccatagcc taaataacca cgggctaccg caactacaac ccgtaaatgc gataggacga
     4741 gtttatgtgc agcttcaata ttgccatcat ttcttagtga gcgagctagg cctgtttctt
     4801 cttcttgtga cagaatgggg caacgattaa ctgcttgaat ataactttcg atattaccac
     4861 cgacgacaga aggtagcgca cagatatcag tcatattatt cctatctcct ttactgttgg
     4921 gatgattatt tgcaaaagat ttatcttgat tttagcactc gctgcataag agtgctaata
     4981 atttttaaag gttcccaaac tgaattgatt tcgttgagaa taaatcgatt gcttcttgtt
     5041 caataaagaa aaaagccata ttttctgcca tatttcagtc aaaacaactc actgttggtt
     5101 caacttattt aacgggggag gggcggtgtt taagtgtgta tcagtgtatc cagtaattca
     5161 atccaatgtt tgacgggtag ggtggttcca ctctgaatgt gcatcaagca gccaatattt
     5221 gcagtaacga tttgcgctgg tccatttgca gataatgcaa ttaccttatc ttttaggagt
     5281 tgttgagata gtgttggctg caaaattgag tatgtacccg ctgatccaca acaaagatga
     5341 gggttatcaa cctgacacaa ggtgataccg gctgaactga ggattttctc aacgacacct
     5401 cgaattttca ttccatgttg cagtgtacag ggtgaatgaa aggcaagctt gccgggatga
     5461 tttagcgatg tttctttcaa tagtaccgtt aaattttcca attcagccgt aatgatttca
     5521 ctaatatctt tagcaagtgc agctactacc tgggcttttt cagcataagc tgcatcatat
     5581 tgcagaaagt gtccatattc tttgacggtg acgccgcaac cactggctga aattacgatt
     5641 gcttcgattt gatgtgtttc gatcaaaggc caccaaacat cgatgttatg gcgcatcgca
     5701 tctaatcctt gttgttgggc attgagatga taggctaacg cgccacagca cccggcttct
     5761 tctaccttga tcaaagaaat acctagttta tcgagaacgc gtgctgtagc tgcattaata
     5821 ttgggagcca gtgtgggttg aacacaacca tccagtacaa gcatttttct tgggtgacga
     5881 gaattgttcc aatttacagc cgaacgctgc aacgggggta tagctgcttt aagtttagca
     5941 ggcataaaag cgcgtaataa ctgcgctatc ctaaccgaaa acttaaatat cgatcgactg
     6001 gttagaaatt tacggattgc aaagcgcttc agctcgctat agcgattacg tgccaccttt
     6061 tcttctacga taccgcggct tatatctaat aggtggctat aacgaacgcc ggaagggcag
     6121 gttgtttcgc aggcccgaca cgtgagacaa cgatccaagt gtaactgcgt tttttgcgta
     6181 accgcttgac cttccagcac ttgcttcatt aaataaattc gaccccgggg gctatccagc
     6241 tcatcgccta ataactggta ggttgggcaa gtcgcaagac aaaatccgca atgtacgcag
     6301 gatcgaagaa tggcttctgc ttcagtacta ttttgatttt tttttacaaa atcagcgagg
     6361 ttggtttgca ttgtttaaaa ctcggagtac atccgtccgt gattaaagat actgagcgga
     6421 tcaaattgtt gttttaattt ttgatgaatt tttagcatag acggggaaag tggatgaaag
     6481 gcttgaatgt ttgatttatc gccataaaat aacgttgcat gcccatttag atcgccaatt
     6541 tgttggcgaa gggtttgatc gttttcttgc ccatcggttg ctaaccagcg taaagccccc
     6601 ccccattcaa tgagttgctc gccttccaat gcgataggta gtagagtaga tttgacggat
     6661 agtcgccaga gtcttttgcc agaagtaaaa aaagggtggg tatggttacg cagcgatcgc
     6721 cagaatgaat cactcgattc gagcagttct ccgcctattt tttggcaagc agtactaacc
     6781 ccagcgggcg aacccgataa cctaatgagt aatttgtttt catgatagca actggctgaa
     6841 atcggtagtg gcttacttgc ccactgatgt attttttcaa tggcttgtga ttgattcatc
     6901 tccaaacaaa tacttaattc agtttccggt cttgggagta cttttaatga agcttctaaa
     6961 atgaccccca aggttcctaa tgagccaacc attaatcgag aaacatcata ccctgcaaca
     7021 ttcttcatga ctcgaccgcc aaatcgtaaa tcattaccct gaccatcgag catgcgtatg
     7081 cctagtacaa aatcgcttac cgagccctga taggcgcgtc taggtcctga caatcctgct
     7141 gcaatggttc cgcctaatgt agtatgctca gtgaagtggg gaggctcaaa agcaagcatt
     7201 tgatttgatt gcagcaacaa tgattctagt tcaaccagtc gagtacccgc tttggcggtg
     7261 ataactaatt cactcggatc ataatcaacg attcctgagt agggagtaac atcaag
//
LOCUS       DAOQKQ010000133         7211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_5107_length_7211_cov_6.153158, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000133 DAOQKQ010000000
VERSION     DAOQKQ010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 7211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7211
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5107_length_7211_cov_6.153158"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PKZ49_09100"
     CDS             complement(<1..309)
                     /locus_tag="PKZ49_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36758.1"
                     /translation="MSSSQKYLIDTNVFIGLEDARQVNAEVASFLALANKHSVGVFIH
                     EAAKDDIARDTDVNRRGISLSKLSKFQILKKVRGLTEQQLEESYGALPKPNDIVDATL
                     L"
     gene            complement(281..1150)
                     /locus_tag="PKZ49_09105"
     CDS             complement(281..1150)
                     /locus_tag="PKZ49_09105"
                     /inference="COORDINATES: protein motif:HMM:NF016178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HND36759.1"
                     /translation="MKFLQDFTYFCLLMNMLNYEKEEAYIMNLVGKIIETYVGVDGNE
                     SLALGKRLQRLRKLAGLTQADFSHRLKISEGALSRLEKRTDIHVSTVKQYVEALGAKL
                     QISAAFSTDNPIAFRIIEAFDAELINEKQLLLPIFDDDVFRPQRDVVLSIKPQYSSKI
                     LKGTKTIELRRRFPIDVPQGTLAFIYSTTPEKALIGYTEIVSVTKESVSNIWRSHKIE
                     ACISKRDFESYFSGQDFGFALRFRSAKPFKQAIDLTELRERFGFEPPQSFLYVKPELR
                     EALRHELLAEISY"
     gene            1360..2085
                     /locus_tag="PKZ49_09110"
     CDS             1360..2085
                     /locus_tag="PKZ49_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HND36760.1"
                     /translation="MAGHSKWANIKHKKAAQDAKRGKIFTRLIKEITIAARLGGGDSS
                     SNPRLRLAIDKAYEQNMPKDNIERAIKRGCGDLEGVSYEEIRYEGYGIGGAAIMVDCM
                     TDNRTRTVADVRHAFTKYGGNLGTDGSVAFLFNHCGQFLYPPGTDENKLIETALEVGA
                     EDIIHHEDDSLEVVTAPYEFITVKTGLEKAGFKAEFAEVTMKPTNETLLSGEDAIKMQ
                     KLLDALENIDDVQDVYTTAVLAE"
     gene            2112..2603
                     /gene="ruvC"
                     /locus_tag="PKZ49_09115"
     CDS             2112..2603
                     /gene="ruvC"
                     /locus_tag="PKZ49_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HND36761.1"
                     /translation="MRILGIDPGLRITGFGVIDKVGNKLTYVSSGCIKTSGDQLPQRL
                     KIILDSLKMIICEHEPDQVAIEHVFVNINPKSTLLLGQARGAAICAAVIHNLMVTEYT
                     ALQIKQAVVGNGHAAKEQVQEMIIRLLNLGGRPGADAADALACAICHAHSLVNNRSNL
                     VLR"
     gene            2616..3197
                     /gene="ruvA"
                     /locus_tag="PKZ49_09120"
     CDS             2616..3197
                     /gene="ruvA"
                     /locus_tag="PKZ49_09120"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HND36762.1"
                     /translation="MIAKISGVLLEKHPPQVLVNVHGIGYEIDVPMSTFYQLPTIGEA
                     VMLHTHFVIREDAHLLFGFSTIEERAAFRQLIKISGIGARTALALLSGLSVLDLQETV
                     VNQDADRLIKVPGIGKKTAERLLLELRDKLNIEVQPCSSDSPETSNNHDILNALFSLG
                     YNEREIGWVIKQIPKGTTVSEGIKQALKLLSKK"
     gene            3228..4259
                     /gene="ruvB"
                     /locus_tag="PKZ49_09125"
     CDS             3228..4259
                     /gene="ruvB"
                     /locus_tag="PKZ49_09125"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HND36763.1"
                     /translation="MIETDRIISAAPSSQKEEVMERALRPKQLEEYIGQEKIREQLQI
                     FIEAARRRNESLDHVLLFGPPGLGKTTLAHIIAREMAVNMRQTSGPVLEKPGDLAALL
                     TNLEPHDVLFIDEIHRLSPVVEEILYPALEDYQLDIMIGEGPSARSVKIDLPPFTLVG
                     ATTRAGMLTNPLRDRFGIVSRLEFYTPQELSKIVLRSAQLMNVDIQLDGAMEIASRSR
                     GTPRIANRLLRRVRDFSEIKAQGHVNQAIADAALKMLDIDEVGLDILDRKFLLAILEK
                     FEGGPVGIDNLAAAINEERDTIEDVIEPYLIQQGYLKRTPRGRVATDLTYQHFKLLPL
                     RDSPNGDLW"
     gene            4308..4733
                     /gene="ybgC"
                     /locus_tag="PKZ49_09130"
     CDS             4308..4733
                     /gene="ybgC"
                     /locus_tag="PKZ49_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HND36764.1"
                     /translation="MSSLPSSPEDKAFSHPVRVYYQDTDTGGVVYHASYLNFLERARY
                     EWLRDIGFSVDSLIKEHKTIFLVRSLNIEYFKPAVLDDLLRVTVEVVDMSRSRITLSQ
                     QVIRDQINLVSATIHVVCVSVDNLRPIRIPTPLFQRINS"
     gene            4740..5423
                     /gene="tolQ"
                     /locus_tag="PKZ49_09135"
     CDS             4740..5423
                     /gene="tolQ"
                     /locus_tag="PKZ49_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HND36765.1"
                     /translation="MSATVTQDMSLLHLVTSASLPVQLVMLMLLIASLLSWWFIFRKL
                     FAIREAIKQSDEFENTFWRGVDLNALYQRAAGARDRAGSMERIFEAGFREFTKHQSGM
                     DIGAIMDSTRGAMRAAYQREMDRLEAHLSFLATVGSVSPYVGLFGTVWGIMNSFRELS
                     NLTQATIAHVAPGIAEALIATAMGLFAAIPAVIAYNRYASDTSQLATRYESFMEELSN
                     VLQRRAVKL"
     gene            5439..5855
                     /gene="tolR"
                     /locus_tag="PKZ49_09140"
     CDS             5439..5855
                     /gene="tolR"
                     /locus_tag="PKZ49_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HND36766.1"
                     /translation="MANLRNKRRLMNDINVVPYIDVMLVLLIIFMITAPLIPPGQVEL
                     PSIGKSSSPPVEPLEVIIAVDGSLTLRDRARAGDEQKVNRTQLVAIIKNKQAQNTEQP
                     VVIAADKNVRYEEVINIMDVLEQEQVKKVGLLIKPK"
     gene            5864..6718
                     /gene="tolA"
                     /locus_tag="PKZ49_09145"
     CDS             5864..6718
                     /gene="tolA"
                     /locus_tag="PKZ49_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HND36767.1"
                     /translation="MTTLAWRRASDEEPGLIRSAALALLVHIVFFGFMVFGLNWRNDP
                     NESMVVDLWRDMPQQPIKPQPVIKEPIKELPKPIPPAQEPVKPQPETVQPKSEPVKIK
                     NPPPRKPVIEIKEKIEPPKPIQEEKKVEPVQEKVPKPEPVKEQVKKPEPIKEVEKKVE
                     PEKELPEKEQKTVEQDVLAQLAAQKSKALDEIAKYKAMIQAKIRSRIVTPPDLPGNPM
                     AEFRVTLLPGGDVLKVSLRKSSGYAAFDEAVERAIFLAKPLPIPPDPALFKEFRDFSL
                     TVYYQDAL"
     gene            6754..>7211
                     /locus_tag="PKZ49_09150"
     CDS             6754..>7211
                     /locus_tag="PKZ49_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HND36768.1"
                     /translation="MRVFTRYCYLALSLLLWSAQVHATLNIEIFGGGSKKIPVAIVPF
                     SQEERLPQSITAIVSADLERTGLFKMVGTFGQTPHEPSDIVYADWQSRGASAIVIGST
                     HLLPDGRVDVRFRLMDVAKTSQITGFTGVVPSDQLRVAAHRIADIIYEALT"
BASE COUNT         2079 a         1396 c         1697 g         2039 t
ORIGIN      
        1 cagtaatgtt gcatcaacaa tatcgttagg cttcggtaac gcaccataac tttcttcaag
       61 ttgttgttct gtcaggcccc gtactttttt aagaatctgg aattttgaga gcttacttaa
      121 cgaaatcccc cgacgattaa catcagtgtc tcgtgctata tcatccttag ccgcttcgtg
      181 aataaagacc ccaacgctgt gtttgtttgc tagcgcaaga aaggaggcaa cttctgcgtt
      241 tacctgtcga gcgtcttcaa gaccgatgaa tacgtttgta tcaataagat atttctgcga
      301 ggagctcatg ccttagcgcc tcgcgcaact caggcttaac atacagaaaa gattggggcg
      361 gttcaaaacc aaagcgttcc cttaattcag ttaaatctat agcctgcttg aatggttttg
      421 cactcctgaa tcgcagtgca aaaccgaaat cttgaccgct aaaataactt tcgaaatctc
      481 gttttgagat acacgcctct attttatgtg agcgccaaat atttgagaca gactccttgg
      541 tgacagaaac tatttccgta taaccaatga gtgccttttc aggcgttgtg gaatagataa
      601 acgcaagtgt cccttgaggg acgtcgatgg gaaatcgccg ccgcagctcg attgtttttg
      661 ttcccttgag gattttggac gagtattgcg gtttgatact taacactaca tccctttgtg
      721 gacgaaaaac atcgtcgtca aatatcggga gcagcaattg tttttcatta ataagttcgg
      781 cgtcgaatgc ttctataata cggaaagcaa tagggttatc tgtagaaaat gctgcactaa
      841 tttgcagttt tgcccctaac gcctcgacat attgtttcac tgtggaaaca tgaatgtcgg
      901 ttcgcttttc taatcgggat aatgctccct cgctaatttt tagtctgtga gaaaaatctg
      961 cttgcgttag cccggctagc ttacgcaacc tttgcaaccg cttgcccaga gcaaggctct
     1021 cgttgccatc aactccgaca taggtctcaa tgatttttcc tactaaattc attatataag
     1081 cttcctcttt ctcatagttt agcatgttca ttaacaggca aaaatatgta aagtcttgga
     1141 gaaatttcat tttttttatt actctattcc ctcaagaaat tgaggcgctt gtaagcttgg
     1201 gtcgatttaa attgcaaatt aattattgca tgagcttatt tctgatggtt ggcgcacaat
     1261 ttattgccgg catactttat tcatgagtaa tggaatgcta ggattacagt cttattggtg
     1321 aagaaccaca atatctagca aactgaggaa gaaaatcaaa tggcgggtca tagtaagtgg
     1381 gctaatatca agcataagaa agcagcacaa gatgcgaagc gcggtaagat ttttacgcga
     1441 ttaatcaaag agattacgat tgctgcaagg cttggtggag gagactcaag cagtaatcca
     1501 cgcttacggt tagcgattga taaagcatat gagcaaaata tgcccaaaga caatatcgag
     1561 agagcgatta aacgaggatg tggtgatctg gaaggcgtta gctatgagga aattcgatat
     1621 gagggctatg gaataggtgg ggccgcgatt atggttgact gcatgaccga taatagaacg
     1681 aggactgtag cagatgtgcg ccatgctttt acaaagtatg gtggcaatct cgggacggat
     1741 ggatcggttg cttttttatt taaccactgt ggccaatttc tttatccacc aggaaccgac
     1801 gaaaataaat tgatcgaaac agcactggaa gtcggcgcag aagatatcat ccatcatgaa
     1861 gacgatagcc tagaggttgt gactgcgccg tatgagttta tcaccgttaa aactggatta
     1921 gaaaaggcag ggtttaaagc agaatttgca gaggtaacga tgaagccgac gaatgaaacg
     1981 ttattgtcgg gtgaagacgc aatcaaaatg cagaaactat tggatgcttt agaaaacatt
     2041 gatgatgttc aagatgtcta tacgactgca gttttagcgg aatagattac ttgcgataaa
     2101 aagggggaga aattcgtatt cttggcatag acccagggtt acgtatcaca gggtttggtg
     2161 tgattgataa ggttggcaat aagctaacct atgtcagtag tgggtgcatt aagacatcgg
     2221 gcgatcaatt gccgcagcgt ttaaagataa ttctcgatag ccttaaaatg atcatttgtg
     2281 agcatgagcc agatcaggtt gcgatcgagc atgttttcgt taatatcaat cccaaatcga
     2341 ctttattgct gggtcaagcg cggggcgctg ctatttgcgc ggcagtcatt cataatctta
     2401 tggtgacgga gtatacggca ttacaaataa agcaagcggt tgtggggaat ggccacgctg
     2461 caaaagaaca agtccaagaa atgattatac gattattaaa tctaggtggt agacctggcg
     2521 ctgatgcagc agacgcattg gcttgcgcaa tttgtcatgc acatagttta gtgaataatc
     2581 gttctaatct tgttctcagg tagtatgatt taaacatgat agcgaaaata tcgggtgtgc
     2641 tgcttgagaa acatcctccg caggtattag ttaatgtgca tgggatcggt tatgaaatcg
     2701 atgtgcctat gagtacgttt tatcagctcc ccaccattgg tgaggcagtc atgcttcata
     2761 cgcatttcgt gattcgcgaa gatgctcatt tgttgtttgg tttttctacc attgaggagc
     2821 gagctgcttt tcgccaatta atcaaaattt caggtattgg tgcaagaaca gcgctcgcac
     2881 tgctatctgg gttaagcgtg ctcgatttac aagagacggt cgtcaatcaa gatgctgata
     2941 gattgatcaa ggttccaggt attggtaaga aaactgcaga aaggctattg cttgaattgc
     3001 gggataaact gaatatagag gtgcaaccct gttctagcga ttcccctgaa acgagcaaca
     3061 atcatgacat actcaatgcg ctattctcgt taggttataa tgaacgagaa ataggctggg
     3121 taattaagca aattcctaaa ggtacgacag tttcggaagg tatcaagcaa gcgctgaaac
     3181 tgctttcaaa aaaataatga ccatctggta gctccaatag aacaacagtg atagaaacgg
     3241 atcgtatcat ttcagcagct ccttcttccc aaaaagagga agtaatggag cgggctctgc
     3301 gtccaaaaca gcttgaagag tatattggcc aagaaaagat cagggagcaa ttgcagattt
     3361 ttattgaagc agctcgccgc cgaaatgaat cacttgacca cgtactgctt tttggtccac
     3421 ccgggttagg caaaactaca ctcgcccata tcattgctag agaaatggca gtgaatatgc
     3481 gccagacttc gggtcccgtt ctagaaaaac caggggattt agctgcttta ctgaccaatc
     3541 ttgagcccca tgacgtcctt tttattgatg aaatccaccg actttctccc gtcgttgagg
     3601 aaatactcta tcctgcactt gaggattatc aacttgatat catgattggg gaggggccat
     3661 ctgctcgatc agtgaagatt gatttacctc ccttcacgct cgtaggagca acaacccgcg
     3721 cagggatgtt gactaatccg ttaagagatc ggtttggtat tgtttcgcga ttagagtttt
     3781 atacgccgca agaattgagt aaaattgtgc tccgttctgc gcaattgatg aatgtggata
     3841 ttcaattgga tggcgccatg gaaatcgcga gtcggtcacg cggcactcca cgcattgcaa
     3901 atcgattatt gcggcgggtg cgtgattttt ctgaaatcaa ggcgcaaggt catgtcaatc
     3961 aagctattgc tgacgcagcc ttgaaaatgt tggatatcga tgaagtaggc ttggatattc
     4021 tcgacagaaa gtttttactg gccattttag agaaatttga gggtggtcca gttggaatcg
     4081 ataaccttgc tgcagccatt aacgaggaac gtgacacgat tgaagatgtg attgaacctt
     4141 acttaattca gcaaggttat ctcaagcgaa caccacgagg gcgtgttgca acagacttaa
     4201 cctatcaaca ttttaagcta ctaccgctta gagatagccc caatggggat ttgtggtgat
     4261 aatttgaaga agaaattgct tcctatcgtt tttttgaaat gaatacaatg agctctttgc
     4321 catcttcccc tgaagataag gcattttcac atcctgtccg tgtttattat caggataccg
     4381 atacaggggg cgttgtatac catgcttcct atctcaattt tttggagcgt gcccgctatg
     4441 aatggttgcg agatatcggc tttagtgttg attccctgat taaagaacat aaaacaatct
     4501 ttttagttcg atcgcttaat atagaatatt tcaaaccggc tgtgcttgat gatttattgc
     4561 gggtcactgt agaggtggtt gatatgagta gaagtcgtat cacattatct cagcaagtca
     4621 tacgtgacca aattaacttg gtgagcgcca cgattcacgt ggtatgtgtt agtgtagata
     4681 acttaaggcc aatccgtatt ccaacacctc tttttcaacg aattaatagt taaattttta
     4741 tgagcgcaac tgtaacccaa gatatgtcac tgttacatct tgtaacaagt gctagccttc
     4801 ccgtccaatt ggtcatgttg atgctgttga tcgcatcttt actttcctgg tggtttattt
     4861 tcagaaaatt gtttgcgatc agggaggcca ttaagcaaag tgatgaattt gaaaatacgt
     4921 tctggagagg cgtcgactta aacgcgctct atcagcgtgc tgctggggca agagacagag
     4981 ccggtagtat ggaacgtatt tttgaagcag gatttcgtga attcactaaa catcaatcag
     5041 gaatggatat tggtgcaatt atggatagca cccgaggtgc aatgagggca gcgtatcagc
     5101 gtgagatgga tcgacttgaa gcacatttat cttttctggc gactgttggg tcggtgagtc
     5161 cttatgttgg attatttgga acagtgtggg gtatcatgaa ttcatttcgt gagttatcga
     5221 atttaacgca ggcgaccatt gcccatgttg cgcctggcat tgcagaagca ctcattgcga
     5281 ctgcgatggg attatttgcg gcaatacctg ctgtcattgc ttataatcga tatgctagtg
     5341 atactagtca gctagctaca cgttatgaga gctttatgga agagctatcg aatgtattgc
     5401 agcgacgcgc tgtaaaactt taggattcta agaagctaat ggctaatctt cgtaataaac
     5461 gtcgtttaat gaatgacatc aatgtagttc cttacattga tgtgatgctg gttttgctca
     5521 tcatttttat gattacagca ccccttatcc caccgggtca ggttgaatta ccttccattg
     5581 gtaaatcatc gagtccaccg gtagagccat tggaagttat tattgcagta gacggcagtt
     5641 taacgttacg tgatcgagca cgtgcgggtg atgaacaaaa agtgaatcgc acacagctgg
     5701 tcgcaatcat taaaaataag caggcccaaa atactgaaca gcctgttgta attgctgctg
     5761 ataagaatgt ccggtatgaa gaagtaatta atattatgga tgttttggag caagaacagg
     5821 ttaaaaaagt aggcttgttg atcaagccaa aataaaagta acaatgacga cattagcttg
     5881 gagaagagct tccgatgaag agccagggtt gattcggtca gctgcactgg cattattggt
     5941 acatatcgtt ttttttggtt tcatggtgtt tggcctcaac tggagaaatg atcccaatga
     6001 aagtatggtt gtggatttat ggagagacat gccacaacag cccattaaac ctcagcctgt
     6061 gattaaggag ccgattaaag agctccctaa accaatccca ccagctcagg agcctgttaa
     6121 acctcaacca gaaaccgttc aaccgaaatc ggagccggta aaaatcaaaa atccaccacc
     6181 gcggaaacct gtgattgaaa tcaaggaaaa aattgagccc cctaagccca ttcaagaaga
     6241 gaaaaaggta gagccggttc aagaaaaggt acccaaacct gaaccggtta aagagcaagt
     6301 caaaaagcca gagccgatta aagaagtgga aaagaaggta gagccagaga aagagttacc
     6361 tgaaaaagag cagaaaacgg tagagcaaga tgtactggct cagttggctg cacaaaaaag
     6421 taaagcctta gatgagattg caaaatataa ggcgatgata caggcaaaga ttagaagcag
     6481 aattgtcaca ccgcctgatc ttcccggtaa tcccatggcc gaatttcgag tgacattgtt
     6541 acctgggggt gatgtgctca aggtttcgtt acgaaaaagc agtggttatg cagcgtttga
     6601 tgaggcggtt gaaagggcta tttttttagc aaaaccgcta cctattccgc ctgatccagc
     6661 tttgtttaag gaatttcgtg attttagtct cacggtatac tatcaagatg ctttataaca
     6721 gcgagtcgat taattaattt gttgagagtt tatatgcgag tatttacacg ctattgttat
     6781 ttagcactca gtttattgct atggagtgct caggttcatg ccacactaaa cattgaaata
     6841 tttggaggtg gttcgaaaaa aattcctgtc gcaattgttc ctttttctca agaagaaaga
     6901 cttcctcaga gcattacggc tattgtatca gccgatcttg agcgaacggg tctatttaaa
     6961 atggtaggca cttttggcca aactccgcat gagccgagcg acattgtcta tgcagactgg
     7021 cagagtagag gggcaagtgc gattgttatt ggaagtactc atctattgcc agatgggcga
     7081 gtcgatgtac gcttccgttt aatggatgta gcaaaaacat cccagattac aggattcacc
     7141 ggcgttgtgc cgtcagatca attacgcgta gccgcccacc gaattgcgga tattatttat
     7201 gaggctttga c
//
LOCUS       DAOQKQ010000134         7195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_5133_length_7195_cov_5.955182, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000134 DAOQKQ010000000
VERSION     DAOQKQ010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 7195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7195
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5133_length_7195_cov_5.955182"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..395)
                     /locus_tag="PKZ49_09155"
     CDS             complement(138..395)
                     /locus_tag="PKZ49_09155"
                     /inference="COORDINATES: protein motif:HMM:NF039366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6171 family protein"
                     /protein_id="HND36769.1"
                     /translation="MDDKCGDCSFPSLSTQGKNLGLSIFNVMTQAIKTGKLRATAETV
                     EKRLEICNGCEFLKDNRCLECGCFLALKAGLHAENCPKGKW"
     gene            complement(388..834)
                     /locus_tag="PKZ49_09160"
     CDS             complement(388..834)
                     /locus_tag="PKZ49_09160"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HND36770.1"
                     /translation="MLELNEQNFDKALQENEQLLVFFYREKGCSFCDKMKPVVEKYAE
                     TRPFTVAKYALGQTPDSVNEKFPIERFPTFYAFQKGQVISKLEGTQTEAKLDSMFAPK
                     IVPIEEAPLSMLLNDEVKLIDAIYPMMLDLKKVQAEIKKRKEIFNG"
     gene            complement(848..3220)
                     /locus_tag="PKZ49_09165"
     CDS             complement(848..3220)
                     /locus_tag="PKZ49_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36771.1"
                     /translation="MDFSPGLKNLIGQPNSWRQVNTYRKDRLGTTSLSLIKLENQATS
                     AAGAGNQQASPALEFIGRGWSTGSSLNTPIAVRQYLQPQSYSTAAALLKFQYNINNTG
                     WNDGFEYTNQTQNGVNIGRFTTYGYLYSSASNSSPIDTLQNVNIVNPSGSQTHLVFTF
                     GSTIRYGMSSSTSGDAKYYSAGSSPFHSFYVGSTITSQSLVAQIYGSGIYNSLNNYNG
                     GKVTAGSADTSPIATLNSYGSLAIKGVSVTSPTYTIAETETLIYANPDQSNVCGGTAP
                     SCSTYTNSSTCGARSAVGCNWFSGTSCSIFNGDINACTGQSPCTLDTLSCSSAANTDQ
                     SSCEALDDSFGGNCSWNTSACPSQPDQTSCESISGCSPEFNNSCSVFSNTVDCNAQTP
                     CSPTIDGDCSALSDGGGDGTNCATQPECSYDNGTGACSGFYFTACNGNYFSACVGNLC
                     DGNYNSGNCSGSFGAECQGSANCGAVTTQTPCNAEPGCEWSQGQVLTLPTTALSNKGG
                     TSRLYGIIHAGTAGTVQINANTGDDIVGLPNIVLTAPYQRVLLHNHQLMGQCSAFLSQ
                     SPCEAQSGCSWANPCGENRDQTSCEAVSGCSWDGGSNTCNGNNYCTGTYTVAKRWFIV
                     NQDADGALFQQYASVTVANTVTETSLLSTGKGITLLPKNYFSKQGVAIRIRARGTHSS
                     TGNPTITFKVKLGATVIGTITGISGNGSGDEWELDSIITCRSIGGSGSVFEQGTFKEL
                     HNNGLEVGSKNTAAVTVSTTVSQQIGLTLTWGTANAGNTLTVTNLSITSE"
     gene            complement(3223..3642)
                     /locus_tag="PKZ49_09170"
     CDS             complement(3223..3642)
                     /locus_tag="PKZ49_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36772.1"
                     /translation="MAGTATPIFPQTIKNYVTQIVNADASNLKTILTGAANGTLLNRI
                     WVCSNDSSARDLQFYVTIGGTDYLIGTLSIPASSGFTNAVPQVGVFESTQFPSMFLDN
                     NGNKVMRIESGAILKVKALTTVTSAKAISIFCQGGEF"
     gene            complement(3644..4459)
                     /locus_tag="PKZ49_09175"
     CDS             complement(3644..4459)
                     /locus_tag="PKZ49_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36773.1"
                     /translation="MSNWSVTVSNTTYTVDVSNAGDIDTVQVTTTPINITVETTGAQG
                     IKGDTGEGVPTGGTTGQVLKKASNTNYDTVWSSGTDTGITELSGDVTAGPGSGSQTAT
                     LTNTAVTPGSYTNASITVDSKGRITSASSGSFDHGNLAGLSDDDHSQYHNDTRGDARY
                     YTKSQVDTAIGTVQSDIDTHELNTSNPHSVTKTQVGLGNVDNTSDADKPVSTAQQTAI
                     DNKVSDSAYDSSGWDAVTTIAPSKNAVRDKIESLVTAISSKIDLATYSQYEGF"
     gene            complement(4456..4815)
                     /locus_tag="PKZ49_09180"
     CDS             complement(4456..4815)
                     /locus_tag="PKZ49_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36774.1"
                     /translation="MAAGKLNLTIEQGSTFTRLLTIKSGGTPVNLSGYSFAGQVRRKH
                     SDQKKLAEFTCAVGSPTSNGQVTISLTATQTSAIPFIYDDDGNLEVFYYDIEMTVGST
                     VDRILEGTVTLSPEVTR"
     gene            complement(4825..5325)
                     /locus_tag="PKZ49_09185"
     CDS             complement(4825..5325)
                     /locus_tag="PKZ49_09185"
                     /inference="COORDINATES: protein motif:HMM:NF017102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HND36775.1"
                     /translation="MVLQSEMLRLLNELSEEAQKWVGTKENDKVKVDLLRKVVDGVAV
                     NEPWCMCFVQFCVRQIEIHCGYQSKIFKSEHCLTTWNKSPAMIRCSKPAVGLIAIWQH
                     GNTASGHTGIVIDVVGENDFTTVEANTTEAPVGIVRDGDGIYIKHRSMVQKSNMRLLG
                     FLNPFM"
     gene            complement(5537..5776)
                     /locus_tag="PKZ49_09190"
     CDS             complement(5537..5776)
                     /locus_tag="PKZ49_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36776.1"
                     /translation="MIQAISAILTGLSNIKYLADLVKQIVEAAMLWYIDNMNAETSKL
                     LADAAAASATAKTDQEHADASALWKKALSRPRIIK"
     gene            complement(5773..6009)
                     /locus_tag="PKZ49_09195"
     CDS             complement(5773..6009)
                     /locus_tag="PKZ49_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36777.1"
                     /translation="MEMLTEFMGKYPAISSILMVIGILRMINKPLFAILKAVADATPS
                     EADNKLLDAAEKSAVYKGFVFVVDWLASVKLIKQ"
     gene            complement(6244..>7195)
                     /locus_tag="PKZ49_09200"
     CDS             complement(6244..>7195)
                     /locus_tag="PKZ49_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36778.1"
                     /translation="VGYSSNVAMSSDTDTRVVAAYIYGSTATGTLNTSDNIVKYPALT
                     RDTHGAYNSSTGQYTVPVPGWYQVNASYELQHASTAAGNEYYISIYTGTSGSTTRKAV
                     AWEAQAVANVPTRRKMRISSRIYCNAGEVIEIRSSTGGTTPSFANVLGGSEFDVALAS
                     GPATIAASESVNLQYSLTSSAPTIGTSPTVLVYNEKVRDSHGSYNSSTGVFTAPRTCV
                     YRITASMRSQSAVSSSSLDHAVYLAVRINGNINKYLSFFTYPVTSVSITPDLKGSVSV
                     KLNAGDTVDIVSARDSGVGSFITSTSGTHSYMMIESVSDL"
BASE COUNT         2016 a         1573 c         1383 g         2223 t
ORIGIN      
        1 aggcgtcttg atcaacccaa acgcccacaa ttagttccga caattctttt cttttgtcat
       61 aattgatccc aatcatttca atatactctg atttaacgcc agtgcgagaa tcggtgaatt
      121 gtttttttag tgccatttta ccattttcct ttcgggcaat tctcagcgtg caaacctgct
      181 tttaatgcta agaaacaacc gcattcaagg caacggttat cttttaaaaa ctcacaaccg
      241 ttgcaaatct ctaaacgctt ttcaaccgtt tcggctgtcg ctcttaactt gccagttttt
      301 attgcttgag tcattacgtt aaaaatcgaa agccccaagt ttttaccttg ggttgagagt
      361 gaaggaaaag agcaatcccc acacttgtca tccattaaat atctcttttc gttttttaat
      421 ttctgcttga acttttttaa gatcaagcat catgggataa atggcatcga ttaattttac
      481 ttcatcattg agaagcatcg aaagcggtgc ctcttcaatg ggaacaattt tgggggcaaa
      541 cattgaatca agttttgcct cggtttgcgt gccctcaagt tttgaaatga cttgcccttt
      601 ttggaatgcg taaaaagtag ggaaacgctc aattggaaat ttctcattta ctgagtcagg
      661 cgtttgccca agggcatact ttgcaaccgt aaatggtcta gtttcagcgt atttttcaac
      721 aacgggtttc atcttatcgc aaaaagaaca tcccttttca cgatagaaaa aaactaaaag
      781 ttgctcgttt tcttgtaatg ccttatcaaa attttgttca ttaagttcta acattttttc
      841 tcacctctta ttcagatgtg atcgaaagat ttgtgaccgt caaggtattt ccagcattag
      901 ctgttcccca agtcagagtt aaaccaatct gttgcgaaac cgttgtcgaa accgtaactg
      961 cggcagtatt cttggagcca acttcaaggc cattattgtg aagctcttta aaagttcctt
     1021 gttcaaaaac tgatccagac ccaccaatgg agcggcatgt tataatcgaa tcaagttccc
     1081 actcgtcacc agacccattt ccagatatgc ctgtaatagt tccaataaca gtagctccaa
     1141 gctttacttt aaatgtaata gttggattgc cagttgaact atgtgttcct ctagctctga
     1201 ttcgaatagc aacgccctgc ttcgagaagt aatttttggg gagtagcgta atcccttttc
     1261 cagtcgacaa aagacttgtc tctgtcactg tattagctac agtcacactc gcatattgct
     1321 gaaacaaagc gccgtctgcg tcttgattta caataaacca acgcttcgct accgtataag
     1381 taccagtgca atagttattt ccattacaag tatttgagcc accatcccat gaacaacccg
     1441 aaaccgcttc gcacgatgtc tgatcacgat tttcgccaca aggatttgcc cacgaacagc
     1501 cgctttgagc ttcgcaaggc gactgcgaaa ggaaagcgga acattggccc atcagttgat
     1561 gattatggag caagactcgt tgatatggtg cggtcaaaac aatatttgga agacctacaa
     1621 tatcgtcacc cgtgttcgca ttaatttgaa cagtgccagc agttccagca tgtattattc
     1681 cataaagtct tgatgtcccg cctttattag ataaagcagt tgttggtaga gttaaaacct
     1741 gaccttgtga ccactcgcac ccaggctccg cgttgcatgg ggtttgagtt gtaaccgctc
     1801 cgcagtttgc tgatccttga cattctgccc caaatgaccc tgagcaattt ccagaattat
     1861 aatttccatc acataaattc ccaacgcacg cgctgaaata atttccatta caagctgtga
     1921 aataaaatcc agagcacgca ccagtcccgt tgtcatagga gcattctggt tgcgttgcgc
     1981 aattagttcc atcaccgccg ccatcactca gcgccgagca atccccgtca attgttggcg
     2041 agcatggggt ttgcgcatta caatcaacgg tattagaaaa cacagagcat gaattattaa
     2101 actcaggcga acacccagaa attgactcac acgacgtttg gtctggttga gaaggacaag
     2161 ctgaagtatt ccatgagcag tttcctccaa atgaatcatc caaagcttcg caactgctct
     2221 gatcagtatt tgccgcactt gagcaagaca aagtgtcaag agtgcatgga ctttgaccgg
     2281 tgcaggcgtt tatatcacca ttaaaaattg aacagctagt tccagaaaac cagttacacc
     2341 caaccgccga tctcgcccca caagtgcttg agtttgtgta tgtcgaacac gatggagccg
     2401 ttccgccaca gacatttgat tgatcaggat ttgcgtaaat taaggtctca gtttccgcaa
     2461 ttgtataagt aggacttgta actgaaacgc cttttatagc aagagatccg taggaattaa
     2521 gagttgcaat tggtgaagta tccgccgacc cagccgttac ttttccgccg ttgtaattat
     2581 ttaatgagtt ataaattccg ctcccataaa tttgagcaac caaagattga cttgtaattg
     2641 tgctgccgac gtaaaaactg tgaaatgggc ttgaaccggc ggaataatat tttgcgtcac
     2701 cggaagtgct cgacgacatt ccataacgaa tagttgaacc aaaagtaaaa actaaatgag
     2761 tttgactccc cgaaggattt actatgttaa cattttggag tgtatcaatc gggctagaat
     2821 tacttgcgct agaatataga tatccgtaag tagtaaatcg cccaatattt acaccgtttt
     2881 gagtttgatt cgtgtactca aacccatcat tccaaccagt attattaata ttatactgaa
     2941 attttaaaag cgccgcagcg gttgagtagg attgtggctg caaatattgt ctcactgcaa
     3001 tcggtgtatt tagagagcta ccagttgacc aaccccgccc aataaattca agcgcgggcg
     3061 atgcttgctg attccctgcc cccgccgccg acgtagcttg attttctaac ttaataagac
     3121 ttagcgaagt cgtacctaat ctatcttttc gataggtatt aacttgtcgc catgagttag
     3181 gctgccctat taaatttttt agaccggggc taaaatccat agttaaaatt caccgccttg
     3241 gcagaaaatg cttatagctt ttgcgcttgt gactgtggtc agagctttaa cttttaaaat
     3301 tgcgcctgat tcaattctca tgactttgtt tccattgtta tcaagaaaca tactcggaaa
     3361 ttgagtgctt tcaaaaactc caacttgcgg gactgcattt gtgaacccac tgcttgcagg
     3421 aatggaaaga gtgccgatta aataatcagt gcctccgatt gttacataga attgcaaatc
     3481 tcttgcgctt gaatcatttg aacaaaccca aattcgattc aaaagagtac catttgcagc
     3541 gcccgttaag attgttttta aattcgatgc atccgcattt acaatttgcg tcacatagtt
     3601 ttttattgtt tgcggaaaga taggggttgc agtgccagcc atattaaaag ccctcgtatt
     3661 gtgaataagt agctagatca atttttgaag aaatcgcggt gactaagctt tcaattttat
     3721 ctctaacagc gtttttactt ggagctatgg ttgtaacagc atcccatccg cttgagtcat
     3781 aggcagagtc tgagacttta ttatcaattg ctgtttgttg agctgtggaa acaggtttat
     3841 ctgcatctga tgtattgtct acgtttccta atccaacctg tgttttagtt acagagtgag
     3901 gattagatgt gttcagctca tgagtgtcta tgtctgactg tactgttcca atagctgtgt
     3961 ctacttggga ttttgtgtag taccttgcgt cacctctagt gtcgttatgg tattgtgaat
     4021 gatcgtcatc tgatagtccg gctaagtttc catgatcaaa tgaaccactc gatgcagacg
     4081 tgattcttcc ttttgagtca acagtaatac ttgcgtttgt atagctacca ggagtaacag
     4141 cggtattagt cagtgtagct gtttgggatc cacttcctgg gccagctgta acatcacctg
     4201 aaagctcagt aattccagtg tcagtgcctg aagaccaaac agtgtcgtag tttgtatttg
     4261 atgctttttt tagaacctgt ccagtggttc caccagtagg tacaccctct ccagtgtctc
     4321 ccttaattcc ttgggctcca gttgtttcaa ccgttatgtt aattggagtc gtagtaactt
     4381 gtactgtatc gatatctcca gcgttagaaa catcaacagt atacgttgtg tttgaaacag
     4441 ttacactcca gttactcatc gagtaacctc tggtgataac gtgacagttc cttctaatat
     4501 tctgtcgaca gttgatccaa ccgtcatttc aatgtcgtaa taaaatactt ctaaatttcc
     4561 gtcgtcatca tagataaatg gaattgcaga tgtttgagtt gctgttaaac tgatggttac
     4621 ttgaccatta gatgttggag atccaacagc gcaagtgaac tcagcaagtt tcttctgatc
     4681 actgtgcttc cttctaactt gaccagcgaa tgaataaccc gataaattta ctggagttcc
     4741 tccagatttt attgtcagta atcgtgtgaa tgtagaaccc tgctcgatag ttaaattcag
     4801 ttttcctgcg gccatgctga aattttacat gaatgggttt aaaaaaccaa gaagtctcat
     4861 gtttgatttt tgaaccattg acctgtgttt gatatatatc ccatccccat ctcttactat
     4921 tcccacaggc gcctctgttg tgttggcttc aacggtggta aaatcatttt ctccaacgac
     4981 atcaattact ataccagtat ggccgcttgc ggtattccca tgctgccata tggctattag
     5041 tcctacagct ggtttgctac atcttatcat ggccggagat ttattccatg tggtcaaaca
     5101 gtgctctgat ttgaatattt ttgattggta tccacaatgt atttcaatct gtctcacgca
     5161 gaattgaaca aaacacatac accacggttc gttgacagcg actccgtcta ctactttcct
     5221 taataaatca accttaacct tatcgttctc tttggtccca acccacttct gagcctcttc
     5281 tgatagttcg tttaagagcc tgagcatttc ggattgtagc accattctat ataaggcttg
     5341 agctgctccg gtttaaagca gaccatcccc tccatttggc ttggaagaag ttttatgtac
     5401 tccaagcatt ttccagacga atctgttttt aggcaataaa aacccatccc aaaaccattt
     5461 agttcataag gatcaatctt tggagctgga tccttgcggc aaccagaaag taggatggtt
     5521 aaaataatta taatcctcat ttaataatcc ttggtcttga tagtgccttt ttccatagcg
     5581 cactggcatc tgcgtgttct tggtcagttt ttgcggtagc tgaggcagct gcagcatcgg
     5641 ctaatagctt tgatgtttct gcattcatat tatcaatgta ccacaacatt gctgcctcaa
     5701 ctatctgctt aaccaaatca gcaagatact ttatgttcga caatccagta agaattgctg
     5761 aaattgcttg tatcattgtt taattaattt gacagaagcc agccaatcaa caacgaaaac
     5821 aaaaccttta tatactgctg atttttcagc cgcatcaagt agcttgttat cagcttccga
     5881 tggagtagca tctgctacag cttttagaat tgcaaacaaa ggtttattga tcatcctcaa
     5941 aattccaatc accattagaa tagaagaaat tgctgggtac ttacccataa attcagttaa
     6001 catttccatt ttaatctcct tttgacgctc caagaacgcc ttcaattcta gctagagatt
     6061 ctcttactct agctagttcc tcgactacct ttgagtctaa agaatcatgt ttagttctaa
     6121 gaatatcaat ttgtttagta gtctcagcat ggtccctatc accttgaatt actctacctt
     6181 caagacgaat tagccatata actataccta acgcactacc gattatgtat tctacactta
     6241 acattatagg tcgctcacgc tttctatcat catataagag tgtgtaccgc tcgttgatgt
     6301 tataaatgaa ccaacaccgc tatctctagc cgaaactatg tcgacagtat caccagcatt
     6361 aagtttcacg gatacagaac cctttaagtc tggagttata gaaacagatg taacagggta
     6421 ggtaaaaaat gatagatatt tatttatgtt tccatttatt ctgactgcta aatagacagc
     6481 gtggtctaac gatgaagaag atacagcgga ctgagaccgc atagaagctg taattctata
     6541 aacacatgtt cgcggcgcgg taaaaactcc agtgctggaa ttataagaac cgtggctatc
     6601 tcttactttt tcattataca ctagaaccgt tggggatgtt ccaatggtcg gggctgatga
     6661 agttagtgaa tattgcagat taacactctc actcgcagcg atagtggccg ggcctgatgc
     6721 taaagcaacg tcaaactcac taccccctag cacgttggca aaactagggg tagttcctcc
     6781 cgttgaagat ctaatttcaa taacttctcc agcattacaa taaattcttg aggagattct
     6841 catttttcta cgagtaggaa cattcgcaac tgcttgggcc tcccaagcaa cagcttttct
     6901 tgtggtggaa ccagaagttc ctgtgtaaat tgaaatataa tactcattac cagcagcggt
     6961 tgaagcatgt tgtaattcat aagaagcatt aacttgatac caaccaggaa caggtacggt
     7021 atattgacca gtgcttgagt tatatgctcc gtgcgtatct ctagttaaag caggatattt
     7081 aacaatatta tcagaagtat ttaatgttcc tgtagcagtt gagccataaa tataagcagc
     7141 aacaacccta gtgtcagtat cagaagacat tgcgacattg ctgctatatc caaca
//
LOCUS       DAOQKQ010000135         7017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_5314_length_7017_cov_5.340563, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000135 DAOQKQ010000000
VERSION     DAOQKQ010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 7017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7017
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5314_length_7017_cov_5.340563"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PKZ49_09205"
     CDS             <1..415
                     /locus_tag="PKZ49_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HND36779.1"
                     /translation="ENIQSQSTSNPTSISQVAAETALNGDQSCMLPMIAAFRERNQFM
                     TDKLNAITGFRCLLSGGAFYAFVDANQAIQSLTKQGLIATANDMDFSEYLLEKSGVAV
                     VPGSAFGCNGYIRLSFATSMDNLTKAMDRLKKLLA"
     gene            complement(606..2378)
                     /gene="arsA"
                     /locus_tag="PKZ49_09210"
     CDS             complement(606..2378)
                     /gene="arsA"
                     /locus_tag="PKZ49_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical pump-driving ATPase"
                     /protein_id="HND36780.1"
                     /translation="MTKLKFLNQPPRFLFFTGKGGVGKTSLACATAIMLADSDLRVLL
                     VSTDPASNVGQVFGITIGNQVTAIAAVPRLAALEIDPQAAAQAYRDRIVGPVRGVLPD
                     TAVKGIEEQLSGACTTEIAAFDEFTALLTDSVLTADYDHIIFDTAPTGHTIRLLQLPG
                     AWSDFLAEGKGDASCLGPLAGLEKQRAQYKAAVDALADPERSRLVLVARAQQATLREV
                     ARTHEELTAIGLTKQFLVINGLLPEAETLQDPLAAAIFQREQKALAAMPEVLNQLPCD
                     HIVLKPFNLVGLTALRKLLIDALPDEGSKEFTHTVILFPSLSNLTDEIAKDGCGLIML
                     MGKGGVGKTTLAAAIAVNLAHRGLPVHLTTSDPAAHLSETLNGAMDNLAVDRIDPSVE
                     TERYRQHVLETKGAHLDAKGRALLEEDLRSPCTEEIAVFRAFSRIIREAGKKFVVMDT
                     APTGHTLLLLDATGAYHREIVKQMDPSIVHYSTPMMQLQNPKQTKIFLVTLAETIPVL
                     EAANLQADLRRAGIEPWAWIINNSVAATTVHSPLLGQRASNELTDIEVVSTAHAKRYA
                     VVPLLKEEPIGVQKLLDLSRSSSQ"
     gene            complement(2398..2748)
                     /gene="arsD"
                     /locus_tag="PKZ49_09215"
     CDS             complement(2398..2748)
                     /gene="arsD"
                     /locus_tag="PKZ49_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter metallochaperone
                     ArsD"
                     /protein_id="HND36781.1"
                     /translation="MTIIHVFDPASCCSSGVCGSDVDQELVGFSADVDWVKQHDVAIE
                     RFNLAQQPMAFADNSTVKAFLERSGADVLPLILVDGEVALAGRYPQRSELARWAGIPL
                     EEKIEESSCCGGCC"
     gene            3149..3553
                     /locus_tag="PKZ49_09220"
     CDS             3149..3553
                     /locus_tag="PKZ49_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HND36782.1"
                     /translation="MIGLLIISHENLGEEFVRCVTHILGERPSNLSCLAVQPNDDPSS
                     TITQAKKLLANLDQGEGVIIITDICGATPCNIASQLMIAGKVVCLAGINLAMLLRILS
                     YRHEPLDVVVSKALDGGKNGVMQILPELPHAS"
     gene            3543..3812
                     /locus_tag="PKZ49_09225"
     CDS             3543..3812
                     /locus_tag="PKZ49_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HND36783.1"
                     /translation="MLRNEVTILNKLGLHARASAKLTKLAGQFDSEVWITRNGRRVNA
                     KSIMGIMTLAANKGSILEIEISGKDEAAAMDAITALINNYFEEGE"
     gene            3809..5539
                     /gene="ptsP"
                     /locus_tag="PKZ49_09230"
     CDS             3809..5539
                     /gene="ptsP"
                     /locus_tag="PKZ49_09230"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HND36784.1"
                     /translation="MSFLLHGVGVSEGIAIGHAHLASPAALDVPHYLLPKNQIDHEVD
                     RLKHAFTTVRQELETLQASVSEGPAFAEFNAFLELHHMILDDPTLSDAAVNCIIQTQC
                     NAEWAIHQQMEVLIAQFEAIEDAYLRERKADVTQVVERILKVLLGMPGYIPPPLKHDG
                     MSILVAHDLSPADVMQYKQHQFAAFLTDLGGVTSHTAIVARSLNIPSIVAMQHARQLI
                     YNNDLLIVDGSRGIVIVNPDKYILAEYRLKKNQLELEKRKLKRLRTLPTITLDGSIIE
                     LHANIELPQDIEPVQESGATGIGLFRSEFLFLNRDDLPNEEEQFAAYRFVAERMQGMP
                     VTIRTFDLGADKNLKQDIHRAATNPALGLRAIRLSLSEPTMFLTQLRAILRASAYGNV
                     RILIPMLSGDLEITQTLQYIEQAKQSLRETQQPFDETIPIGGMIEIPAAALCVESFMR
                     KLDFLSIGTNDLIQYTLAIDRVDETVAHLYDPLHPAVLWLLSKIIHSANLENIPVSLC
                     GEIAGDIQYTRLLLGMGLKQFSMYPAQILTIKQEILKSHLPSIRPLTKKILKAEEPEK
                     IRQLLSRLNN"
     gene            5682..6809
                     /locus_tag="PKZ49_09235"
     CDS             5682..6809
                     /locus_tag="PKZ49_09235"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HND36785.1"
                     /translation="MHDSDSIGIVTPQRLHFDSPLQLESGAILDNYELVYETYGELNA
                     ARSNAVLICHALSGNHHVAGVYADNPKNAGWWNNMIGPGKPIDTNRFFVIGVNNIGGC
                     HGSTGPTTINTKTNKPFGADFPMVTTIDWAETYVRLAQQLNIDQFAAVVGGSLGGMSA
                     MQLSLDAPEKVRHALVIAAAAKLTAQNIAFNDVARQAIITDPDYHGGDYYAQQTFPRR
                     GLRLARMLGHITYLSDDSMAEKFGRELRSGSLGYNFDVEFQIESYLRYQGDKFAEQFD
                     ANTYLLMTKVLDYFDPARDYDNDLSAAFHCVKANFLVLSFSSDWRFSPERSRSIVKAL
                     LDNNLNVSYAEIASRHGHDSFLMKDDHYHQLVRSYMNNITT"
     gene            6851..>7017
                     /locus_tag="PKZ49_09240"
     CDS             6851..>7017
                     /locus_tag="PKZ49_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HND36786.1"
                     /translation="MIMTEITDTISPQLRPDFATIAAWIKPATKVLDLGCGDGALLRY
                     LTDTRQIKGYGV"
BASE COUNT         1914 a         1665 c         1525 g         1913 t
ORIGIN      
        1 ggaaaatatc caatcgcaga gtacctctaa cccaacgtcg atctctcaag ttgcagcaga
       61 aaccgcgctc aatggcgatc aatcctgcat gttacccatg atcgcagctt tccgcgaacg
      121 caatcagttc atgaccgata aactcaatgc gattactggg tttcgatgct tactctccgg
      181 cggtgctttc tatgcatttg tggatgccaa tcaggccatt caaagtttaa caaaacaagg
      241 cctgatagcg actgccaacg acatggattt cagcgaatat ttactggaaa aatcgggggt
      301 ggctgtagtg ccaggatccg cctttgggtg taatggctac atccggcttt cgtttgcaac
      361 ctcgatggat aatctcacta aagcgatgga tcgcctcaag aaattgctag cgtgagtgcg
      421 gattcggcat acattgattt atgcactacc atagcgttaa gaaatatgct tgctcggtga
      481 attcgaactc aacacttcaa gatgttcagc aagccatact ttaataatgg gctgccgcgg
      541 cctcatgcac gccaattcaa aagcacggta aaaaacagcg acaccacttt tctatccatg
      601 aattgttatt ggctactgct ccttgataaa tccaataact tctgcacgcc gatcggctct
      661 tctttgagta atggcacaac cgcataacgt tttgcatggg cagttgatac cacttcgata
      721 tcagtaagtt cattgctggc gcgctggccc aataagggtg aatgaacagt tgttgcagca
      781 acactgttat taatgatcca cgcccatggc tcaataccag cacggcgtaa atcagcttgt
      841 aggttggcag cttccagtac cggtatcgtt tccgctaatg taaccaggaa tattttggtt
      901 tgcttagggt tttgtagttg catcattggt gttgagtagt gcacgatgga agggtccatt
      961 tgtttgacaa tttcgcggtg ataagcgccg gtcgcatcca gtaataataa ggtgtggccg
     1021 gttggtgcag tgtccatcac gacaaacttc ttacccgctt cgcgaattat tcttgagaat
     1081 gcccgaaata ccgcaatttc ttcggtacaa ggcgagcgca aatcttcttc caataaagcc
     1141 ctgcctttag catctagatg tgcgccctta gtttccagga catgctgacg gtagcgttca
     1201 gtctccacag agggatcaat ccgatctacc gctagattat ccattgctcc attgagcgtt
     1261 tcactcaaat gtgctgcagg atctgaagta gtcaaatgta ctggcaaacc tcgatgtgcc
     1321 agattcacgg caatagcagc agccagcgtg gttttaccaa caccgccttt gcccatgagc
     1381 atgattaaac cgcacccatc tttggcaatt tcatcagtca agttagataa gctggggaat
     1441 aggattacag tatgcgtaaa ctctttgctg ccttcgtcag gcaaagcatc aatcagcaat
     1501 ttccgtaagg cagtcaatcc gactaggtta aacggtttaa gtacaatatg gtcgcaaggt
     1561 agttggttga gaacttccgg catggctgcc agcgcttttt gttcgcgctg aaaaattgca
     1621 gcagctaaag ggtcctgtaa tgtttcggct tctggtaaca agccattgat gaccagaaac
     1681 tgtttcgtca agccaatagc tgttaattct tcatgcgtcc gcgctacctc gcgtagcgta
     1741 gcttgttgcg cacgagccac caataccaat cgactccgct ccgggtcagc aagagcatct
     1801 accgctgctt tgtattgggc gcgctgtttt tccagaccag ctaatgggcc aagacaagag
     1861 gcatcaccct tgccttccgc aagaaaatca ctccatgcgc cgggcaattg cagcagccga
     1921 atggtgtgac cggtcggcgc ggtatcgaag ataatatgat cgtagtccgc agtcagcacc
     1981 gagtccgtca atagtgcggt aaattcatcg aaggcagcta tctcggtcgt gcatgcgccg
     2041 gataattgct cttcgatacc tttgactgct gtatccggca acacgccacg caccggaccg
     2101 acgatgcggt cgcggtaagc ttgggctgcc gcttgcgggt caatttccaa ggcggctaaa
     2161 cggggtactg cggcaatcgc cgtaacctga tttccaatcg tgataccgaa tacctgacct
     2221 acgttagagg ctggatcagt gctgaccaat aatactcgca aatcggaatc agccagcatt
     2281 atcgctgtgg cgcaagccaa tgatgttttt ccgactccgc ctttgccggt aaaaaataga
     2341 aaacgcggtg gctgattgag aaatttgagt ttggtcacga gcaaacctga gcttgcttca
     2401 gcaacaaccg ccgcaacaac tcgattcttc aattttttct tcaagaggaa tccccgccca
     2461 acgcgcgagt tcgctacgct gcggatagcg gcctgccagt gccacttccc catctacaag
     2521 aattaaaggt agcacatcag caccagatcg ctccaagaat gcttttaccg tagaattatc
     2581 cgcaaaggcc atgggttgct gtgctaaatt aaaacgctcg attgcaacgt cgtgctgctt
     2641 gacccaatct acatcagccg aaaaaccgac cagttcctgg tctacgtcag aaccgcaaac
     2701 gcctgagcta cagcatgagg cagggtcaaa tacatggata attgtcattt tgattctcct
     2761 ataaagttaa actaacaagg ttgcgataca atttaattct acttttagct attagcgcat
     2821 agcaccgcaa agctgacctc agattttaat cgctccagaa ctcctgggta aatatcgcat
     2881 ttattttttc attacaccat cccctcaaaa aacagcgtct cttacaaact tgggacgatt
     2941 caaatttcat gaactccata tttgacaata cacctcaaac accaagtaaa gtgcatttat
     3001 atggccctaa actcgctgat atattttgta agtcttgagt catataaaga tttttgtagc
     3061 ctctcccaaa ataacctaac atgactactt acgaactcgc gtgttaatgt tattttattc
     3121 actatttcaa actaccaaga agaagataat gattggccta ttaattattt ctcatgagaa
     3181 tctgggagag gaatttgtgc ggtgtgtaac acatatttta ggagagcgac ctagcaactt
     3241 gagttgcctc gctgttcaac cgaatgacga tcccagttca acgataaccc aagcaaaaaa
     3301 attgcttgct aatttagatc aaggagaagg tgtcattatc atcaccgata tctgtggcgc
     3361 tacaccgtgt aatattgcca gtcagctcat gatcgctgga aaagtcgttt gtttggctgg
     3421 aatcaattta gcgatgctat taaggatttt gagctatcgc catgaacccc tcgacgtggt
     3481 tgtatccaaa gcactcgatg gcgggaaaaa tggcgtcatg caaattttac cggagttacc
     3541 gcatgcttcg taacgaagtt actattctca acaaactcgg cctacatgcc cgtgcttcgg
     3601 caaaactcac caaattagca gggcaattcg atagcgaagt atggataacc cgcaatggtc
     3661 gccgcgttaa tgcaaaaagt attatgggta tcatgacatt agcagctaat aaaggttcga
     3721 tactggaaat cgaaatttca ggtaaggatg aagcggcggc aatggatgcg atcaccgccc
     3781 tcattaacaa ttattttgag gaaggtgaat gagctttcta ttacatgggg taggtgtttc
     3841 tgaaggcatc gcgatcggac atgcacatct cgcctcccct gcggcattgg atgttcctca
     3901 ttatttattg cctaaaaacc aaatcgacca tgaagtggat cgcctcaaac atgcattcac
     3961 gaccgttcgt caagaattgg aaacgctgca agcttctgta agcgagggac ctgcctttgc
     4021 tgaattcaat gcattccttg aattgcatca tatgattctg gatgatccga cgttgtccga
     4081 tgcggctgtt aattgcatta tccaaaccca gtgcaacgca gaatgggcga ttcatcagca
     4141 gatggaagta ctcattgcgc aattcgaagc catcgaagac gcctatctcc gtgaacgaaa
     4201 agcagacgtt acgcaagtgg ttgagcgtat cttaaaagtg ttgctcggga tgcccggtta
     4261 tatcccccct ccgcttaagc atgatggaat gagtatttta gttgcacatg acctaagtcc
     4321 tgcggatgtc atgcaatata aacaacatca atttgctgcc tttctgacag atctgggtgg
     4381 agtaacctct catactgcca tcgttgcgcg cagcttaaat attccatcga tcgttgcgat
     4441 gcaacatgca cgccaattga tctacaacaa tgatctgtta attgttgatg gtagtcgtgg
     4501 aatcgtgatt gtcaatccag ataagtacat tctggctgaa tatcgcctca agaaaaatca
     4561 actcgaatta gaaaaacgta agcttaaacg acttagaacg ttgccaacca tcacgcttga
     4621 tggcagtatc atcgagttac atgcaaacat tgaacttcct caagatattg aaccggtgca
     4681 agaaagtggc gcgaccggta tcggtttatt tcgcagcgaa tttctttttc taaaccgtga
     4741 cgacctcccc aatgaagagg agcaatttgc agcttatcgc tttgttgctg agcgcatgca
     4801 agggatgccc gtcacaatcc gcacatttga cttgggcgct gacaaaaacc tcaaacagga
     4861 cattcatcgt gctgcaacca atcctgcatt gggattgcgt gcaatccgat tgagtttatc
     4921 agaacccacc atgtttctga cgcaattacg cgccattctg agggcatctg cctatggtaa
     4981 tgtgcgcatt ctgattccaa tgctttcagg tgatttggaa attactcaga ctttgcagta
     5041 catcgaacaa gccaagcaat cgttacgaga aactcagcaa cctttcgatg aaaccattcc
     5101 gatcggtggc atgattgaaa tccctgcagc tgcgctctgt gtcgagtcat ttatgcgcaa
     5161 acttgatttt ttatctatcg gtaccaatga tctcatccaa tatacacttg cgattgatcg
     5221 ggtcgatgaa acggttgcgc atttatatga ccctctccac cccgcagtac tttggctatt
     5281 atctaaaatc atccattcgg cgaatctgga gaatatccca gtatccctct gcggtgaaat
     5341 tgccggtgat attcagtaca cgcgattatt gctcgggatg ggcttgaaac aattctcgat
     5401 gtatccagct caaattctca ccattaaaca agaaatatta aaaagccatt taccctcgat
     5461 tagaccgctg accaaaaaga ttctgaaagc agaagagcct gaaaaaatac gccaacttct
     5521 gtctcgtttg aataattaac cattcgcttt ttttccatct aatgcaagtg agttttatcc
     5581 gagaagatcg catttgcttt gccaaacgct atattattct caagaaaata cgtatgatta
     5641 accttttatt gatacgcttt cactcgtaaa ccgctcgacg tatgcatgat tcagattcaa
     5701 ttgggatagt taccccgcaa cgcttacatt ttgactcccc cctacaactc gaaagtggcg
     5761 ctatattgga taactatgag cttgtctatg aaacttacgg agaactcaat gcagcacgat
     5821 ccaatgcagt gctgatttgt catgcgctat caggcaatca tcatgttgca ggcgtttatg
     5881 ctgacaatcc gaaaaatgct ggttggtgga ataatatgat tggcccagga aagcccattg
     5941 ataccaatcg tttctttgtc atcggtgtca ataacatcgg aggctgccat ggttcaactg
     6001 gacccactac cattaatacc aaaacaaata aaccatttgg cgctgatttc ccaatggtta
     6061 ccactatcga ctgggctgaa acctatgtac gtttagcaca gcaactcaat attgaccaat
     6121 ttgcagcagt cgtcggtggg agtctaggcg gtatgtccgc tatgcagtta tcgttggatg
     6181 cgcctgaaaa agtccgacat gctttggtta ttgctgctgc agcgaaatta acggcacaaa
     6241 atatcgcgtt taacgatgta gcacggcaag cgattattac cgatcctgat taccatggtg
     6301 gcgattatta tgcacaacaa acctttccac gaaggggatt acgtctagcc cgcatgctag
     6361 gtcatattac ttatttatca gatgattcga tggctgaaaa atttggaaga gaacttcggt
     6421 ctggttcact gggttacaat tttgatgtgg aatttcaaat tgaatcctac ctgcgctatc
     6481 aaggtgataa attcgcggag caatttgatg ccaataccta tttgttaatg actaaggttc
     6541 ttgattattt tgatcccgct cgcgattatg ataatgatct cagcgcagct ttccactgcg
     6601 taaaagcaaa ctttttagtc ctttcattta gctcagattg gcgtttctcg cctgaacgct
     6661 cacggagcat cgtgaaagca ttgctggata acaacctcaa tgttagctac gctgaaatcg
     6721 cttctcgcca cggccatgat tcgtttctaa tgaaggatga ccactatcac cagttagtac
     6781 ggtcttatat gaataacatc acgacttaat cgcgcatcga ctcgcttaat tacgggtagc
     6841 tttgacttca atgatcatga ctgaaataac cgataccatt tctcctcagc ttagacctga
     6901 ttttgccacg attgcagctt ggattaagcc cgcgactaaa gtactcgacc ttggttgcgg
     6961 cgatggtgcg ctattacgct atcttactga tacacgccag atcaaaggat acggtgt
//
LOCUS       DAOQKQ010000136         6946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_5394_length_6946_cov_6.606153, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000136 DAOQKQ010000000
VERSION     DAOQKQ010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 6946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6946
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5394_length_6946_cov_6.606153"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..96
                     /gene="ffs"
                     /locus_tag="PKZ49_09245"
     ncRNA           <1..96
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKZ49_09245"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            156..1763
                     /gene="dnaX"
                     /locus_tag="PKZ49_09250"
     CDS             156..1763
                     /gene="dnaX"
                     /locus_tag="PKZ49_09250"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HND36787.1"
                     /translation="MILTQVLARKWRPKVFTELAGQDHIVKAIANSLDRNRLHHAYLF
                     SGTRGVGKTTIARILAKALNCDQGITSQPCGQCTTCLSIDQGTFVDLVELDAASNTQV
                     DHMRELLESAYYAPSVARFKIYLIDEVHMLSKSAFNAMLKTLEEPPNHVKFILATTDP
                     QKLPITVLSRCLQFNLKQIPPALIVSRLQHILSREQIKADLPALQLIARAACGSLRDA
                     LSLLDQAIAYGNGVVEENQVRDMLGTIDLDYLYDLLEALSTQDGRAMIAIADQMEARS
                     LSFDSALQDMGVLLHRLALAQIAPQAIDEAMLDRERFFDLVKRFSPEEIQLYYQIVLH
                     GRHDLSLAPDDYAGFTMTLMRMLAFVPEQDLASFEYNADDKKAEPAILIPNAKPVDEK
                     PVTHDSSQAEQWADSDYTSQQWLSIVKQLKLSGMAKMLAQHCEAKFLSEEKIALYVPE
                     LHKHLLEKRYQDRLKQALEDYFKKTIDIKFSIGTLSGMTVAAIEDQEKQLKQSQAIAA
                     IEADPFVREVVENFDAKLIVSSIKPIN"
     gene            1780..2103
                     /locus_tag="PKZ49_09255"
     CDS             1780..2103
                     /locus_tag="PKZ49_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HND36788.1"
                     /translation="MKANLGNIMKQAQQMQENMKAMQEQLASIEIEGQSGAGMVKVVM
                     TCRHDVKRVSIDPSLLGDDKEMLEDLIAAAVNDAVRRVETTTQEKMSGLTGGLGLPPG
                     FKLPF"
     gene            2172..3068
                     /locus_tag="PKZ49_09260"
     CDS             2172..3068
                     /locus_tag="PKZ49_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HND36789.1"
                     /translation="MRTSRPLRSPRKPSQPTENRSQPKRVDQAVTHKLQKLLAQSGLG
                     SRREMEALIAEGQVSINGKVAKVGDRISTGDLIRVKNRLIRINTGSRLPRIMFYHKPE
                     GEIVSCHDPEGRPSVFDKLPQLKSSRWVSIGRLDFNTSGLLIFTTSGELANQMTHPRF
                     QIEREYAVRIIGQLTPEQMTQLTRGVELEDGIAKFDMIVEQGGEGSNRWYHAVIKEGR
                     NREIRRMFEKLGIAVSRLIRVRFGPIRLPPYLKRGMLSELKENEVRQIMALFNKMIEP
                     KIVVPRVQSNQSKFTSNRFSST"
     gene            complement(3040..3963)
                     /gene="prmB"
                     /locus_tag="PKZ49_09265"
     CDS             complement(3040..3963)
                     /gene="prmB"
                     /locus_tag="PKZ49_09265"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HND36790.1"
                     /translation="MTHSNNAAPTLIQQAKTELRTVRDWLRLTTSYFSQAQLFFGHGF
                     PTAYDEAAHLILHVLHLAPNQLDLFLDAQIIESERNAMIELIEKRVSERIPITYLTHE
                     AWLGDFNFYVDQRVIIPRSFIAELLQTQLSPWISNPISVTRALDLCTGSGCLAILLAH
                     AFENAQIDAVDISPSALEVAHINIENYDLASSIHLIHSDLFSKLGNKRYDLIISNPPY
                     VNAESMDDLPEEFRHEPSIALAGGFDGLAIVRNILSNAADFLNDEGLLIVEIGHNRSA
                     LEQLYPRMPFTWLETSAGDQFVFMLKKNDLK"
     gene            4067..4549
                     /gene="tadA"
                     /locus_tag="PKZ49_09270"
     CDS             4067..4549
                     /gene="tadA"
                     /locus_tag="PKZ49_09270"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HND36791.1"
                     /translation="MMDEEWMRLALGLAEKAQELDEVPVGAIIVKNGEIIGQGYNQPI
                     KTNDPSGHAEMIAIRAAGQQIQNYRLEECTLYVTLEPCAMCIGAIFHARLRRLVFAAS
                     DPKTGVCGSILDLPAEKRLNHHLEVHKGLLAEESARLLRQFFYGKRHLDKLARYENSN
                     "
     gene            4533..5012
                     /locus_tag="PKZ49_09275"
     CDS             4533..5012
                     /locus_tag="PKZ49_09275"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HND36792.1"
                     /translation="MKIQINIEQQQLDLFDREERLVKRYLISSAKNGIGQVRGSYCTP
                     LGKHIIRAKIGDKQPVNTVFVKRRPTGEIYTPQLAAQYPGRDWILTRIFWLSGCEVGF
                     NRLGIVDTMRRYIYIHGSPDSAVMGKPGSIGCIRMHNDDLLELFDKVPVGTSVNITQ"
     gene            complement(5108..6376)
                     /locus_tag="PKZ49_09280"
     CDS             complement(5108..6376)
                     /locus_tag="PKZ49_09280"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HND36793.1"
                     /translation="MSLVATVIPIEKASSKILVSMFMIYTAFICVSQSWANDRQETAK
                     QERVLLKADRIFDGRTLHEDHALLIDNGKVIEIGPAPQIRKRGGKELDLGDATILPGF
                     IDLHAHLALQNVSHHAVLRHGVTTVRDVGGPLRAPAGGDGSLRVLTTGPIITAPGGYP
                     IPVFGQHGHGGGDHGHSDVALVVETPDQAREAVRHLIQGGAVNIKIALEPGGEPGAPW
                     TTGHAPSVMPPWPLLPLEIIQAIVDETHTLGKKVTAHVGEQQGVELALAAGVDEWAHV
                     PCMEISGDLLQQAVQQGVRIVTTIDTLSLCPGVYSNTLRLAQLGAQFYYGAEIAHIEI
                     PWGIDARELQLMLHLTGMTPLALFETATAKAGEALGLSPLGTLIVGAPADIIAVKGNA
                     FENVKLLEYPDLVISGGRLIVNHFPSKKAK"
     gene            6709..>6946
                     /locus_tag="PKZ49_09285"
     CDS             6709..>6946
                     /locus_tag="PKZ49_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36794.1"
                     /translation="MKQVKLISVLLSTLIFLIISSAIVMAKSSELEFRGAVTNIAIPD
                     GDGDSIVKVKLITSHSPDFEVPIIVLGATEIESNG"
BASE COUNT         1980 a         1377 c         1625 g         1964 t
ORIGIN      
        1 gggcctcccc gcattgtacg gtagtgaacc tggtcaggtc cggaaggaag cagccacaac
       61 tattttatac aagtgccggg ggtcaggctc gccaccttcc aaactttgat acgcagcgtt
      121 tccttttcgt ttatcaacct tcctcaaagt atactgtgat cttaacccaa gtgcttgcca
      181 gaaagtggcg acctaaagtt tttactgaac tggcagggca ggatcatatc gtcaaagcaa
      241 ttgcaaattc gcttgatcgg aatcgtttgc atcatgccta tttgttttcg ggaacgcgtg
      301 gtgttggtaa aacgacaatc gcccgaattt tagcgaaggc cctcaattgt gaccaaggca
      361 ttacctctca accctgtggg caatgcacga cttgccttag tattgatcaa ggaacctttg
      421 tagatttagt tgaattagat gccgcgtcga atacgcaggt cgatcatatg cgagagctac
      481 ttgaaagcgc ttattatgcg ccatccgtgg cgcgttttaa gatttatctg atcgatgaag
      541 tccatatgct ttctaagtcc gcatttaatg caatgctgaa aacacttgaa gaacctccca
      601 atcatgttaa gtttattcta gcgacgacag atccccagaa attgcccatt accgtacttt
      661 cgcggtgcct gcaatttaat ttgaaacaaa ttccacctgc gctgatcgtg agccgattac
      721 agcatatctt atcacgagag caaataaaag ctgatttacc agcgttacag ctaatcgcga
      781 gagcggcctg tgggagtcta cgcgatgcac ttagcttact cgatcaggca atcgcctatg
      841 gaaatggtgt ggtcgaagaa aatcaggttc gagatatgtt aggaacgatt gatctggatt
      901 atctctatga tttgcttgaa gcattgtcaa ctcaagatgg gcgtgcaatg atagcgattg
      961 cagatcagat ggaggcccgt agcttatcgt ttgatagcgc gttacaagac atgggggtat
     1021 tattgcatcg tctcgcttta gcgcagattg cgcctcaagc gattgatgaa gccatgctag
     1081 atcgcgagcg tttctttgac ttggtaaaac ggttctcgcc tgaggagatc caactgtatt
     1141 accagattgt gcttcacggt cgtcatgatt taagcttagc accagatgat tatgccggtt
     1201 tcacaatgac gcttatgcgc atgctcgctt ttgttccaga gcaagattta gctagctttg
     1261 aatacaatgc agatgataaa aaagctgagc cagcgatact catacctaat gctaaaccag
     1321 ttgatgaaaa gcctgtcact catgattcca gtcaagcaga gcagtgggct gatagcgatt
     1381 atactagcca acagtggttg tccattgtta agcagctaaa gttgagtggt atggcgaaaa
     1441 tgcttgcaca gcattgtgaa gcaaaatttt tgtcagaaga aaaaatagcc ttatacgttc
     1501 ctgaacttca taaacatttg ttggagaagc gttatcaaga caggcttaaa caggccttgg
     1561 aagattattt taaaaaaaca atagatataa aattttcgat tggtaccctt tcaggaatga
     1621 ccgttgcagc gattgaggac caagaaaaac aacttaagca atcgcaggcc attgctgcga
     1681 tcgaggcgga tccctttgtc agagaggtgg ttgaaaattt cgatgcaaaa ttaattgttt
     1741 cttcaattaa acctataaat taaaggagag aatcaaatga tgaaagcaaa tttaggcaat
     1801 atcatgaagc aagcacagca aatgcaggaa aatatgaaag cgatgcagga gcaacttgct
     1861 agtatagaaa tagagggtca atctggggca ggtatggtta aagttgtgat gacttgtcgc
     1921 cacgatgtca agcgagtaag tatcgatccg agcttgttgg gagatgacaa agaaatgctg
     1981 gaagacttaa ttgcagccgc tgtgaatgat gctgttcgtc gagtcgaaac gacaactcag
     2041 gaaaaaatga gtggcttgac gggcggtcta ggattaccgc caggatttaa attacccttt
     2101 tgattttcta tcagaacgga cggtatgttg tatcgtgaat cataatgaat tgctctgctt
     2161 gaaggtgttt tatgcgaaca agtcgccccc ttagatcacc tagaaaaccg tctcaaccaa
     2221 cggaaaatcg atcgcaaccg aaaagagttg accaggccgt tacacataag ctgcaaaagc
     2281 tattagcgca aagtggttta ggttcgaggc gggaaatgga agcattgatt gctgagggac
     2341 aagttagtat caatgggaaa gttgctaaag ttggggatcg aattagtacg ggtgacttaa
     2401 ttcgggttaa aaaccgactg atccgaataa ataccggtag tcgtcttcca agaatcatgt
     2461 tctatcataa accagaaggt gagattgtta gttgccatga tccagaggga agaccttcag
     2521 tctttgataa gctgccacaa ttaaaatctt cgcgttgggt ttcgattgga agattagatt
     2581 ttaataccag tggtttattg attttcacga caagcggtga attagcaaat caaatgacgc
     2641 acccacgctt ccagattgag cgcgaatatg cagtacggat cattggtcaa ttaacgccgg
     2701 aacaaatgac tcaattgact cgtggcgttg agcttgaaga tgggatagcc aagttcgata
     2761 tgattgtcga gcaagggggt gaaggttcca accgatggta ccatgcggtc atcaaagaag
     2821 gccggaatcg agaaattaga aggatgtttg agaaactggg aattgcggtg agtcgcctga
     2881 ttcgtgttcg gtttggccct attcgtttac caccttactt gaaacggggc atgttgtccg
     2941 aactcaaaga aaatgaagtt cgccaaataa tggctttatt taataaaatg attgaaccaa
     3001 agatcgttgt tcctcgggtt cagtccaatc aatcgaaatt tacttcaaat cgtttttctt
     3061 caacataaat acgaactgat caccagcact tgtttcaagc caagtaaacg gcattctagg
     3121 atatagctgc tccaaggcag accgattatg gcctatttcc acaatgagta ggccttcatc
     3181 atttagaaaa tctgctgcat ttgacaaaat gttacgaaca atggcaaggc catcaaaacc
     3241 tccggctaga gcaatggagg gttcatggcg aaactcttcg ggaagatcat ccattgattc
     3301 cgcattaacg tagggtggat tgctgataat gagatcatag cgcttgttac ccagcttaga
     3361 gaaaagatcc gagtgaatca agtgaatgga agaagctaaa tcatagttct caatattaat
     3421 atgggctact tctagcgcgc tgggagaaat atcaaccgca tcaatttggg cattttcgaa
     3481 agcgtgcgcc agtaagatcg cgaggcaacc cgatcccgtg cagagatcta atgcgcgagt
     3541 caccgaaata gggttgctga tccaaggtga cagctgggtt tgtaacaatt cagcaataaa
     3601 cgatctcggt ataatgacgc gttgatcgac gtaaaaatta aaatctccta accatgcttc
     3661 gtgggtgaga taagtaatag gtattctttc agagactcgt ttttcaatta attcgatcat
     3721 tgcattacgc tcggactcaa tgatttgagc atctagaaat aaatcgagtt gattcggcgc
     3781 aagatgtaac acgtgtaaaa taaggtgtgc tgcttcatca taagcggttg gaaagccgtg
     3841 tccaaagaat agttgtgctt gactaaagta gctagtagtg aggcgtagcc agtcgcgtac
     3901 agttcgtaat tctgttttgg cttgttgaat tagggtgggg gcagcattgt ttgagtgtgt
     3961 cacgatcaat ctagatagaa gtttgtgata aaaaattgta taagtttaga atgatagcgt
     4021 ggaatggttg gatatcgaat aaattgtgat tttagatagc ctgggtatga tggacgaaga
     4081 atggatgcgg ttggcgcttg gattagcgga aaaagcgcaa gaattggatg aagtccctgt
     4141 tggtgcaatc atcgtcaaaa acggtgaaat tattggtcaa ggttataacc agccgatcaa
     4201 aacaaatgat ccctcgggcc atgctgaaat gattgcgatt cgcgctgccg gtcaacagat
     4261 tcaaaattac cggttagaag aatgcacgct ttatgtcacg ctggagcctt gcgcgatgtg
     4321 tattggtgca atttttcacg cccgattgcg ccgccttgtg tttgctgcaa gtgatcctaa
     4381 aacgggtgta tgtggaagta ttttggattt gcctgcggaa aaaaggctta atcatcatct
     4441 ggaagtgcat aaaggtctgc ttgctgaaga gtcagctcga ttgttgaggc agttttttta
     4501 tgggaagcgt catttagata aactagctcg gtatgaaaat tcaaattaat attgaacaac
     4561 agcagcttga tttgtttgat cgtgaagaaa gattggttaa acgctattta atttcttcgg
     4621 ctaagaatgg catagggcaa gtaagaggga gttattgtac gcccttgggt aagcatatca
     4681 ttcgtgcaaa aattggcgat aagcaaccgg ttaacacggt tttcgtcaaa cggcgaccca
     4741 caggtgagat ttatacgccc caattagcgg cccaatatcc tggccgagat tggattctga
     4801 cacgaatctt ttggttatcg ggttgtgaag tcgggtttaa ccggctagga atagtcgata
     4861 ccatgcgtcg gtatatttat attcacggga gcccggatag tgcggtaatg ggtaaacccg
     4921 gttcgattgg ctgtattcgt atgcataacg atgatctatt agagcttttc gataaagtgc
     4981 cggttgggac atctgtcaat atcacgcaat gatttttaca ttgctgagcc taaatagcta
     5041 aatcattgca gtcttatgag aattcactcg ttattgcaac gatttagctg attgtttgat
     5101 cgtccgatta tttggctttc ttagaaggaa aatgattaac aattaatcga ccacctgata
     5161 ttactaaatc agggtactcc aacagtttaa cattttcaaa ggcatttcct tttactgcaa
     5221 tgatgtcggc aggcgcacca acaatgagcg tgcccagtgg tgatagaccc aatgcttcac
     5281 ctgctttagc agttgcggtc tcaaataacg ctaatggcgt cattcctgtc agatgaagca
     5341 tcaattgtaa ttctcttgca tcaatacccc atggtatttc gatatgggct atttctgcgc
     5401 cataataaaa ttgtgcgcca agttgagcca gccttagcgt atttgaataa acgccaggac
     5461 ataaagacaa ggtgtcaatg gtcgtcacaa ttcttacccc ttgttgtact gcttgctgaa
     5521 gtaaatcccc cgagatttcc atacagggaa catgcgccca ttcatctact ccagcagcga
     5581 gcgctaactc aacgccctgt tgctcaccta cgtgtgcagt tacttttttt ccaagtgtgt
     5641 gcgtctcatc gacaatcgct tggattattt caaggggtaa taagggccag ggtggcatca
     5701 cggatggcgc gtgcccagtt gtccatggtg cgcccggctc tcctcccggt tcaagcgcta
     5761 tttttatatt aactgctcca ccttgaataa gatgtcgaac ggcttccctt gcttgatctg
     5821 gggtctcaac tactaaagca acatcgctat ggccgtgatc accgccgcca tggccatgct
     5881 ggccaaagac agggattgga tagccgccag gtgctgtgat tatcggtccg gttgtcagaa
     5941 cacgcaggct gccatcgcct cctgctggtg cccgcaatgg cccacctaca tcccgaacag
     6001 ttgtcacacc atgacgaaga actgcatgat gagaaacatt ttgcagcgct agatgagcat
     6061 gtagatcgat aaaaccaggc agaatagttg catctcctaa atctagttcc tttccaccac
     6121 gcttgcgaat ttgtggggct ggtccgattt cgatcacctt gccattatcg attaataacg
     6181 catgatcttc atgcagtgtg cgtccatcaa aaatacgatc tgcttttaat aagacgcgtt
     6241 cttgctttgc tgtttcctgg cggtcatttg cccaactttg cgatacacag atgaatgctg
     6301 tgtatatcat gaacatactt actaagattt tacttgatgc tttctcgatt ggaattacag
     6361 ttgcaaccaa actcatcatt tcttcctttt tgttgtagtt atttgaattt attttcttaa
     6421 ataatgcagc gacgtgtggc cattatagga ataaaaatta atgatggaat gtgacaaatt
     6481 gtcgcagcac aattgactgc gattattatc tgaatactgc cagcattaaa tctggccgcg
     6541 tcgaattttt ttacagccta agcattaata gatagacaaa tctagattaa tcattaattt
     6601 gtagaaatgg catagatatt gcttttttta cttggtacaa gtatccacaa ttttcggttg
     6661 agatttttta taaacaaaaa agtattttgg agttctctag aggaaataat gaaacaagta
     6721 aaattgatct cagtattatt atcaacgctg atatttttga taatctcttc tgccatcgtg
     6781 atggcaaaga gttcagagct tgagtttaga ggtgctgtaa cgaatatcgc tattcctgat
     6841 ggcgacggtg atagcattgt taaagtgaaa ttgattacca gccactcccc tgattttgaa
     6901 gtgcctatca tcgtgttagg tgccacagag attgagtcga atggtt
//
LOCUS       DAOQKQ010000137         6918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_5428_length_6918_cov_5.849483, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000137 DAOQKQ010000000
VERSION     DAOQKQ010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 6918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6918
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5428_length_6918_cov_5.849483"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="PKZ49_09290"
     CDS             complement(<1..822)
                     /locus_tag="PKZ49_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HND36795.1"
                     /translation="MSTRLSKHALKVLFVTSEVYPLCKTGGLGDVSAALPASLREQQV
                     DVRILIPGYPQVLAGLKYKRKVAEFNDLAHFPPAKLLSANLQVNASTSIPVFVIDCPS
                     LYQRTGGPYLDVAGHDWPDNAQRFGLLSKMGALLGSDASPLTWQPNVVHCNDWQSGLT
                     PAYLHFHRGLKSPTLMSIHNLAFQGCFAPELVSQLGLPSESYSVQGVEYYGSLSFIKA
                     GLYYADHISTVSPSYAHEIQQERLGFGLHGLLSARKEQISGIVNGIDTIEWDPSAD"
     gene            complement(856..2502)
                     /gene="pgi"
                     /locus_tag="PKZ49_09295"
     CDS             complement(856..2502)
                     /gene="pgi"
                     /locus_tag="PKZ49_09295"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HND36796.1"
                     /translation="MTSLTQSLVWLALKAHQSKLESQHLRSLFAEDPHRFEKFSIRFN
                     DFLLDFSKQPVTNETMNLLFKLATQQKLNDWIAQMFRGEKINVTEHRAALHIALRANQ
                     TVTTESVDILPAVKQVLQQMESFTQAVHQGIHRGYSGLTFTDVVNIGIGGSDLGPVMV
                     TEALKPYWNGQITPHFVSNIDGAHLAETLKALNPATTLFVIASKTFTTLETIANAHAA
                     RTWFLTQGGSEKEIARHFVAVSTNQKAVQQFGIDPANMFVFWDWVGGRYSLWSAIGLS
                     IALTLGMQHFYDLLSGARQMDQHFLTTPFAENLPVILGLIGIWQINFLGATSHAILPY
                     DQSLHRFPAYLQQLEMESNGKSVTRDGTRVDYQTGTVVWGESGTNGQHAFYQLLHQGT
                     PTVTADFLAPCQSHYAVDHHHQLLLANFFAQTEALMLGKTADEVQVELQSQGYTLSEI
                     EQLAPHRVFAGNHATTSILFKKLDPKTLGSLIALYEHKVFVQSVIWNINPFDQWGVEL
                     GKQLANKIMTDLKTDQKVTAHDASTNGLINYFKENSDISN"
     gene            complement(2522..3724)
                     /locus_tag="PKZ49_09300"
     CDS             complement(2522..3724)
                     /locus_tag="PKZ49_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase family protein"
                     /protein_id="HND36797.1"
                     /translation="MSREIMVLSGVRTAIGDYGGALKDIPPTALASKVVREAVNRAQV
                     DISRIGHLVFGHVIHSEASDMYLSRVAAIQGGLPQSVAALSVNRLCGSGLQAIISAAQ
                     TILSGDTDIAIAGGVESMSRAGYLLPALRWGQRMRNSSAIDMMVGALTDPFDQVHMGN
                     TAENIAAKWNIGREKQDQFALESHRRATQAIRNGYFTSQILPIEISSRGKVVVFDTDE
                     HPRDLIDLEKLSNLPSVFQENGTVTAGNASGINDGAAAVVLCDATTAESDHLQPIARL
                     VAYGYAGVDPRYMGIGPVPAVHQALHRACLTIDDMDVIESNEAFAVQACAVACELGFD
                     PIKTNPNGGAVAFGHPIGATGCVLTIKALYELHRIKGRYALVTMCIGGGQGIAAIFER
                     LPSFAQLN"
     gene            complement(3763..5091)
                     /gene="hslU"
                     /locus_tag="PKZ49_09305"
     CDS             complement(3763..5091)
                     /gene="hslU"
                     /locus_tag="PKZ49_09305"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HND36798.1"
                     /translation="MSQMTPQEIVHELDKHIIGQINAKRAVAIALRNRWRRQQIADPL
                     RQEITPKNILMIGPTGVGKTEIARRLAKLADAPFIKVEATKFTEVGYVGRDVDSIIRD
                     LVESAIKQGREQEIRKMRPLAEDRAEERILDVLLPPARDLDFRAENEDSNATRQKFRK
                     KLREGELDNKEIEIEIAGAQASMEIFAPPGMEELTSQIQSMFQNMGSSKKKARKLMIR
                     DAKKILIEEEAARLINDEELKLRCVQNVEQNGIVFLDEIDKITSRSETAGADVSRQGV
                     QRDLLPLVEGTTISTKYGMIKTDHILFIASGAFHLAKPSDLIPELQGRFPIRVELTSL
                     TAADFEQILTNTDACLTRQYEALLKTEGVILNFAESAIKRLAEIAFSVNEKTENIGAR
                     RLHTVIEKLLEEISFNAAKHDGETLLIDAAYVDNRLNELAQSEDLARYVL"
     gene            complement(5123..5644)
                     /gene="hslV"
                     /locus_tag="PKZ49_09310"
     CDS             complement(5123..5644)
                     /gene="hslV"
                     /locus_tag="PKZ49_09310"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HND36799.1"
                     /translation="MTTIVSVRRGKQVALGGDGQVTLGAVVAKSSARKVRRLYHGKIL
                     AGFAGGTADAFTLFERFEGKLEKHQGHLMRSAVELAKDWRTDRMLRRLEAMLVVADHE
                     ATLIITGAGDVIEPELSLAAIGSGGAYAQSAARALLENTELSAKDIVSKALTIAGDLC
                     IYTNQAHIIESLD"
     gene            5787..6857
                     /locus_tag="PKZ49_09315"
     CDS             5787..6857
                     /locus_tag="PKZ49_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HND36800.1"
                     /translation="MPNLMSELHLATMTLSNRIIMAPLTRCRASTDHVPNALMAEYYA
                     QRASAGLIIAEATMIMAGHSAFWKEPGIYSEAQINSWQQITTAVHRAGGRIFLQLWHG
                     GRACHPLLNGGAQPISASAIPILGDEVHTPEGKKPYVTPREIADSEIPSIITGFKTAA
                     ENAKAADFDGVEIHGANGYLLDQFLRDGANKRHGPYGGSIEKRARLMLEVIEAVSQVW
                     ESHKVGLRISPLNSYNDMIDSDPISLTRWLASHLNDYNLAYLHLIRGDILQKQKGDII
                     TPARTHYKGILIGNQGYTPQEAEYAVKEGIVDAIAFGVPFIANPDLPLRIKNKAPLNQ
                     ANPARFYTSGPEGYTDYPFWQP"
BASE COUNT         1917 a         1662 c         1426 g         1913 t
ORIGIN      
        1 atcagcggat ggatcccact cgatggtgtc aatcccattc acaatcccac tgatctgctc
       61 tttgcgagca gataataaac catgcaaccc aaacccgagt cgttcctgct ggatctcgtg
      121 cgcatagcta gggctaaccg ttgaaatatg atccgcataa taaagccctg ctttaataaa
      181 tgacaaactt ccataatact cgacaccttg gacgctataa ctttcagaag gtagcccaag
      241 ctggctcacc agctcgggtg cgaaacaacc ctggaatgcc aagttatgaa ttgacattag
      301 ggtaggcgat ttcaaacccc gatgaaaatg aaggtaagcg ggcgtcaagc cactttgcca
      361 atcattgcag tgaacaacat tgggttgcca cgtcaatgga ctggcatcac tgccaagcaa
      421 tgcacccatt tttgagagca agccaaaacg ctgtgcatta tcgggccaat cgtgtccagc
      481 cacatctaaa tagggccctc ccgttctttg atataacgaa ggacaatcga tgacaaaaac
      541 tgggatactt gttgaggcat tgacttgcag gttggctgat aataacttag cagggggaaa
      601 atgagctaaa tcattgaatt cagctacctt tcgtttatat tttaatcctg caagcacttg
      661 cggataacca ggaatgagga tgcgcacatc aacttgttgt tcacgtaacg acgccggtaa
      721 tgccgcactc acatcaccta gtcccccagt tttgcataac ggataaactt ccgacgtaac
      781 aaaaaggact tttagcgcat gcttcgagag acgggtagac atttatttat taaatgtatt
      841 gatctatcaa cgatttcaat tcgagatgtc gctattttct ttgaaataat tgattaatcc
      901 attggttgat gcatcatgag cagtaacttt ttggtcggtt ttcaaatcag tcataatctt
      961 gttagcaagc tgctttccta attctacacc ccattgatca aatggattga tgttccaaat
     1021 gacactttgc acgaaaactt tgtgttcata gagcgcaatt aacgaaccca atgttttcgg
     1081 atcaagtttt ttgaatagaa tcgacgtcgt cgcatgattc cccgcaaaaa cccgatgcgg
     1141 tgccaattgt tcaatttcag aaagcgtata accttgcgat tgtaattcta cttgcacttc
     1201 gtcagcagtc ttgccaagca ttaatgcttc tgtttgtgca aagaaattag ctaataataa
     1261 ttgatgatgg tgatccacgg cataatgact ctggcaaggt gctaagaaat ccgcggttac
     1321 agtcggtgtc ccttggtgga gtagctgata aaaagcatgc tggccgttag ttcccgattc
     1381 cccccaaaca acggtaccag tttggtagtc aactcgtgtt ccatctcggg taacactttt
     1441 accgttactt tccatttcaa gttgctgtag ataggctgga aatcgatgca gcgactgatc
     1501 gtacggtaaa attgcgtggc tagttgcgcc cagaaagtta atctgccaaa ttccgattaa
     1561 acccaaaatt accggaagat tttcagcgaa aggcgttgtc aggaaatgtt gatccatttg
     1621 cctcgcacct gataacaagt cataaaaatg ctgcataccc agggtcaagg caatcgataa
     1681 tccaatcgca gaccacagtg aataacgccc cccgacccaa tcccagaaaa caaacatatt
     1741 ggcaggatcg attccaaatt gttggaccgc tttttgattc gttgaaactg ctacaaaatg
     1801 acgtgcaatc tccttttcag aaccgccctg tgtcaaaaac caggtacgcg ctgcatgggc
     1861 gttagcaata gtttctagtg tcgtaaaggt ttttgaagcg attacgaata acgtcgttgc
     1921 tggatttaac gcttttagtg tttcggcaag atgtgcccca tcaatattcg atacgaaatg
     1981 aggcgttatt tgcccattcc agtaaggctt taacgcttct gtgaccatga ctggcccaag
     2041 atcagaacca ccaataccaa tattgaccac atcggtaaaa gttaaccctg agtaaccacg
     2101 atgaataccc tgatgaactg cttgtgtaaa gctttccatt tgctgcaaaa cttgctttac
     2161 cgctggcaaa atatccacac tttcagtcgt aactgtttga ttagcccgca atgcaatatg
     2221 aagggcggca cgatgctctg taacgttgat tttctcccct cggaacattt gagcaatcca
     2281 atcattaagc ttctgctgtg ttgcaagctt aaacaataaa ttcatggttt cattcgttac
     2341 gggttgctta ctaaaatcaa gcaagaaatc attaaaacga atcgagaatt tttcgaaacg
     2401 gtgtggatct tctgcaaaca ggctacgtaa atgctgcgat tcaagcttag attgatgtgc
     2461 ttttaaagca agccatacga gtgattgggt caaggatgtg atcatcgtgg catctacctt
     2521 tctaatttaa ctgtgcaaac gagggtaatc gttcaaaaat agctgcaatg ccttgcccac
     2581 cgccgatgca catcgtcact aatgcatagc gccccttgat acgatgcagt tcataaaggg
     2641 ctttgatcgt cagaacacat cccgtagcac cgattggatg tccgaaagca acagcgccgc
     2701 cattgggatt ggttttgatc ggatcgaaac cgagctcaca agcaactgcg caggcctgta
     2761 cagcaaaagc ttcattggat tcgataacat ccatatcatc aatcgttaaa caagcacggt
     2821 gtagtgcttg atgtactgct ggaacagggc ctatacccat ataccgaggg tcaacaccgg
     2881 catagccata ggctactaat cgtgcaatgg gttgcaagtg atcgctttct gcagttgtgg
     2941 catcacaaag cacaacagcc gctgcgccat cgttgatacc tgacgcatta cctgcggtaa
     3001 ccgtgccatt ttcctggaaa acactaggta agtttgataa tttttctaga tcgatcaagt
     3061 cacggggatg ttcatcggta tcaaaaacaa ccactttgcc acgcgatgat atttcaatcg
     3121 gtagaatttg cgaggtaaag taaccgttac gaatcgcctg tgtcgcacgt cgatggcttt
     3181 ccagcgcaaa ctgatcttgt ttctcacgtc caatattcca ttttgccgcg atgttttcgg
     3241 ctgtatttcc catatgcact tgatcaaagg gatcggtcag ggcaccgacc atcatgtcaa
     3301 tcgctgaact atttctcatc cgttgacccc atcgcagcgc aggcaaaaga taacctgccc
     3361 ggctcatcga ttctactccg cctgcaatgg caatgtcggt atcacccgat aggatagttt
     3421 gcgctgctga gataatcgct tgcaagccgc ttccacataa tcgatttacc gataatgccg
     3481 caacagattg tggtagcccc ccttgaatag ccgcgactcg tgacagatac atatcactcg
     3541 cttcactgtg aatgacatgg ccaaaaacca aatgaccaat tctcgatata tcaacctgag
     3601 cgcgattgac tgcttctcgt acgacctttg atgccagcgc agtgggcgga atatctttga
     3661 gcgcaccgcc gtaatcccca atagcagtac gcacaccact taacaccata atttcgcgcg
     3721 acatgatgag ggtaaaaacc tttttatacc aacttatttc gattaaagaa catagcgtgc
     3781 aagatcttcg ctttgagcta attcgttaag tcgattatca acataagccg catcgatcaa
     3841 cagggtttcg ccatcatgct tagctgcatt aaatgaaatc tcttcaagta atttctctat
     3901 cacggtatgt agccgccttg caccgatatt ctcagttttt tcattaaccg aaaaagcaat
     3961 ttcagctaat cgcttgattg cactttccgc aaaattcagt atgactcctt ctgttttaag
     4021 cagtgcttca tactgacgcg tcaggcacgc atcggtattg gtaagaattt gctcaaaatc
     4081 agccgctgtc aggcttgtca gctcaacccg tatcggaaaa cgtccttgca actcaggaat
     4141 aaggtcagaa ggtttagcca agtgaaatgc accgcttgca ataaacaaga tatgatcagt
     4201 cttaatcatg ccatatttgg ttgatatcgt cgtgccttca accaaaggta ataagtcacg
     4261 ttgtactcct tggcgtgaaa catccgcccc cgccgtctcg gaacgactcg taattttgtc
     4321 gatttcatcc aagaatacaa ttccattttg ttccacgttc tgcacgcacc ttaacttcaa
     4381 ttcttcatca ttgatgagtc gagctgcttc ttcttcaatg agaattttct tcgcatcacg
     4441 tatcatcaac ttacgtgctt tctttttgct agaacccatg ttctggaaca tactttgaat
     4501 ttgtgaagtc aactcctcca tcccaggtgg tgcaaaaatc tccatgcttg cctgtgcacc
     4561 agcgatttca atttcaatct ctttattatc gagctcacct tcgcgtaatt ttttacgaaa
     4621 tttctggcgc gttgcgttgg aatcctcatt ctcagcacga aaatccaggt cacgagcggg
     4681 cggcagtaaa acatcaagaa ttcgttcttc tgcgcgatct tcggcgagtg gtctcatttt
     4741 tcgtatttct tgctcacgcc cctgcttaat tgcggactca accaaatcac gaataatcga
     4801 atcaacatca cgccccacgt aaccgacttc agtaaattta gtcgcttcga ctttaataaa
     4861 aggtgcatca gctaatttag ccagacggcg cgcaatttca gttttgccta ccccggtagg
     4921 cccaatcatg agaatatttt taggcgtgat ctcttgacgt aatggatcag caatctgctg
     4981 gcgacgccag cgattgcgca gtgcaattgc aactgcacgc ttggcattga tttgtccaat
     5041 aatatgttta tccaattcgt ggacaatttc ttgtggggtc atttgtgaca taaatttatt
     5101 gcttattaaa aaattcaatc aattaatcga gagactcgat gatatgcgct tgatttgtgt
     5161 aaatacacaa atccccagcg atggtcagcg ctttcgagac gatatcttta gcagaaagct
     5221 ctgtgttttc aagcaaagca cgcgctgctg attgcgcata ggcgccacca ctcccaattg
     5281 cagcaagact aagctccggc tcaatcacat cacccgcacc agtaataatc aacgtggctt
     5341 catgatcagc tacaaccagc attgcttcaa gacgccgcag catgcgatcc gttcgccaat
     5401 ctttggctag ttccaccgct gatcgcatca agtgaccttg gtgcttctcc aatttacctt
     5461 caaaccgctc aaatagcgtg aacgcatccg ccgttcctcc ggcaaaccca gccaaaattt
     5521 ttccatggta aagccgtctt actttgcgcg cgctcgattt agcaaccacg gcaccgagcg
     5581 taacctgccc atcccctcct agagcaactt gcttaccgcg cctcactgat acgatggtag
     5641 tcatgttatt tattattaga ttaaagtact aattatagcg ctgattctaa ggggttatga
     5701 aattgtaaaa aaagcgtgcg gagataattt agaccttgct ttagaatttc tgctaggctt
     5761 gaaactttat caattctatc ttaatcatgc ctaatttaat gagtgagctg catctagcaa
     5821 caatgacact ctctaatcga attatcatgg caccgctcac acgctgtcga gctagtactg
     5881 accacgtacc taatgccttg atggcagaat attatgcaca acgcgcaagc gctggcctga
     5941 tcatcgctga agcaactatg atcatggcgg gccattctgc tttctggaaa gaacccggta
     6001 tttattctga agcacaaatt aacagttggc agcagataac cacagccgtt catcgtgcag
     6061 gggggcgaat ctttttacaa ctttggcacg gtggccgtgc atgccatccg ctgcttaatg
     6121 ggggcgcgca acctattagc gctagcgcca ttcccattct aggtgatgaa gttcacacgc
     6181 ctgaaggaaa gaaaccatac gttactccgc gtgaaatcgc tgatagtgaa ataccttcca
     6241 ttatcaccgg atttaaaact gctgctgaaa atgcaaaagc tgctgatttc gatggtgttg
     6301 aaattcatgg tgccaatgga tatctactgg atcaattttt acgcgatggc gcaaacaaac
     6361 gccatggtcc ttatggtggc tcaatagaaa aacgagccag actcatgctg gaagtcattg
     6421 aagcggtcag tcaggtctgg gaaagccaca aagtggggtt acgcatctca ccgcttaaca
     6481 gctacaacga tatgattgat agcgatccaa taagtttgac tcggtggtta gcaagtcatt
     6541 tgaatgatta taatcttgct tatttacatt tgattcgagg cgatatcttg cagaaacaaa
     6601 aaggggatat cattacacct gcacgcactc attataaggg tatcttaatc ggtaaccaag
     6661 gctatactcc acaagaagct gagtatgccg tcaaagaagg catcgtcgat gcaatcgctt
     6721 ttggagtccc ctttatcgct aatcctgatt tgccgttgcg aattaaaaac aaagcaccgc
     6781 tcaatcaagc aaatccagct aggttttata catctggacc tgaaggttat accgattatc
     6841 ctttttggca accttaattt tagtttatgc ctctaaacta ttttcagttt aatctgggtg
     6901 tgactcaaac aggcattg
//
LOCUS       DAOQKQ010000138         6878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_5478_length_6878_cov_6.421955, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000138 DAOQKQ010000000
VERSION     DAOQKQ010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 6878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6878
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5478_length_6878_cov_6.421955"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /gene="tolB"
                     /locus_tag="PKZ49_09320"
     CDS             <1..997
                     /gene="tolB"
                     /locus_tag="PKZ49_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HND36801.1"
                     /translation="PDGRVDVRFRLMDVAKTSQITGFTGVVPSDQLRVAAHRIADIIY
                     EALTGDMGVFSTRIAFVAKTGNKFALQIADSDGYNAHSIIEYSEPIISPAWSPDGSKI
                     AYVSFENKKPVIYVQTLATRERKAVANFKGSNSAPAWSPNGDKLAVVLTLHGGSQIFL
                     INADGSGLQRLAQSPGIDTEPNFSPDGRSIIFTSDRGGSPQIYRMAISGGNAGVAERL
                     TFEGSYNVSPRFSPDGKSFTYIHRNGDRFNIALQGLANRQVQLLTDSKFDESPSFAPN
                     GKMILYATEISGRGILSVVSSDGQTKQRLSSQTGDIREPSWGPLLKSQRNNHFIN"
     gene            1015..1527
                     /gene="pal"
                     /locus_tag="PKZ49_09325"
     CDS             1015..1527
                     /gene="pal"
                     /locus_tag="PKZ49_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HND36802.1"
                     /translation="MKKLLTVLIICLLSACASQNKQSAEVDDRAMAGGGSGSQDGAGA
                     GGFGLSPLRDPNNILSQRSVYYDYDSYTIKSEYRELVLAHARYLRDNGDAKVLLQGNT
                     DDRGSREYNLALGQKRANSVKNAMLLSGARDQQIEAVSLGEEKPRATGHGESAWAENR
                     RADILYQGEY"
     gene            1527..2330
                     /gene="ybgF"
                     /locus_tag="PKZ49_09330"
     CDS             1527..2330
                     /gene="ybgF"
                     /locus_tag="PKZ49_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HND36803.1"
                     /translation="MVLRASSFLVVALLLVSKPSIAGLFGDEKAREQIDLLQKQTQAM
                     EARIAGMEELLKSNALIELYSQVEGFKIELGKLTGQIEVLTEENKLLRKQQKDFYLDL
                     DNRLNQVESEGSRESGNTSLGEPDSSTALPSEIEMTAQTVDGSEREAYQTGYNLFREG
                     DYLAAISHFEQFLKQYASSSLAPGAAYWIGNAHYALRNFQEAISAQQRVIDQYPKSAK
                     APDALLNIASSQLEIPDVSAAKKTLENLIQKYPNSEAAGKAKKRLTSLK"
     gene            2376..3017
                     /gene="queE"
                     /locus_tag="PKZ49_09335"
     CDS             2376..3017
                     /gene="queE"
                     /locus_tag="PKZ49_09335"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HND36804.1"
                     /translation="MEKLRVSEIFYSLQGETTRMGLPTVFIRLTGCPLRCVYCDTQYA
                     FTGGKMMSIPEILDKVSNSKARYVTVTGGEPLAQKACLSLLTALCDAHYSVSIETSGA
                     FDISEIDPRVSRIVDIKTPGSGEVSKNLFENLALISSKDEIKFVLCDREDYQWALQIM
                     NDYQLAQVCPILFSPTYGQLAADLLAKWILEDHAPVRLQLQLHKILWGEVAGR"
     gene            3057..3749
                     /gene="queC"
                     /locus_tag="PKZ49_09340"
     CDS             3057..3749
                     /gene="queC"
                     /locus_tag="PKZ49_09340"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HND36805.1"
                     /translation="MKKAVVLVSGGLDSATTLAVARDQHFDCYALSIDYGQRSRSELD
                     AALNVGKMIGVTDHQVISVDLRNLGRSALTDQSIDVPTTGMQVGIPVTYVPARNTIML
                     SLALAWAESLASQAIFIGVNAIDYSGYPDCRPDYIEAFERMANLATKAAYEGVKLKIH
                     APLIDLSKREIILLGTRLNVDYSRTVSCYQADEHGLACGVCDACRLRKMGFSAAGISD
                     PTRYSPIYREPQ"
     gene            complement(3764..4135)
                     /locus_tag="PKZ49_09345"
     CDS             complement(3764..4135)
                     /locus_tag="PKZ49_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HND36806.1"
                     /translation="MDVIFLQNFRTKAMIGLYPWEKKVAQLIELNLEIALPTRSASAS
                     DDIKDTIDYSLIADRIKHTLSDKHFMLLEALAEHIAQLILAEFKSPQVKVSVAKLGII
                     KGIEKLGVSIVREFDSHQHEN"
     gene            4226..4840
                     /gene="plsY"
                     /locus_tag="PKZ49_09350"
     CDS             4226..4840
                     /gene="plsY"
                     /locus_tag="PKZ49_09350"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HND36807.1"
                     /translation="MATIILLLMAYLLGSIPFAMVASKLFDLPDPRTYGSKNPGATNV
                     LRSGKIAAAVVTLLGDAGKGWLAVELTRVYVSMEGGGYELISGAALLVFLGHLFPIFL
                     NFKGGKGVATAAGVLLGFNVLLGSLVILTWVIVAAISRLSSLSALTAAVLAPIYSYLI
                     FNWDTPVWAVIIISLLLMLRHQANIINLIEGKETRIGKKATDKT"
     gene            complement(5123..5425)
                     /locus_tag="PKZ49_09355"
     CDS             complement(5123..5425)
                     /locus_tag="PKZ49_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36808.1"
                     /translation="MKNPELKLNINGSYERARLDKVRADKEQLELDLARRNLVLRSDV
                     HNAAFEAGRKLRDTLHSVCKQSAPNLVNIEDPAKIEMYLRDEVDRALNEFIKSCEL"
     gene            complement(5654..6427)
                     /locus_tag="PKZ49_09360"
     CDS             complement(5654..6427)
                     /locus_tag="PKZ49_09360"
                     /inference="COORDINATES: protein motif:HMM:NF013258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HND36809.1"
                     /translation="MAGSHLFRMGTVKGKNGVLIALKHNKRALQAERGASANINPFRT
                     PLNYSLTKPDNAEAIDRHAKVLMVQAGIDQPRKNQVMAVEAIFSLPIDQHQQDTRPFF
                     QCCMDWIVRNIPGVLLSFDVHLDETAPHAHALILPLVDNKMQGNKIMGGKGNLMRLIN
                     LFHAEVARYYGLSRNETKRLTNKAKQSLERQVLAKLEIDPVMKSLIWSCVRDAIHDNP
                     LPFAQLLGIEQKLAKYTGRSFVQIMTSKGKGEEIELNTI"
     gene            complement(6431..6574)
                     /locus_tag="PKZ49_09365"
     CDS             complement(6431..6574)
                     /locus_tag="PKZ49_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36810.1"
                     /translation="MNPDTWLEQLEMLLIRFSHLGIGADIDSMSLIELWAVYRYLLRL
                     EDG"
     gene            complement(6564..6863)
                     /locus_tag="PKZ49_09370"
     CDS             complement(6564..6863)
                     /locus_tag="PKZ49_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HND36811.1"
                     /translation="MDKKIPPHKEAAFKEINQQFTGDDAKQQCARLLQALRLFNINTF
                     EASRGLGIYHPPARILQLRKNGFCIDTIWQVIEAENGDKHRVGCYLLRNGDSHES"
BASE COUNT         1920 a         1343 c         1564 g         2051 t
ORIGIN      
        1 gccagatggg cgagtcgatg tacgcttccg tttaatggat gtggcaaaaa catcccagat
       61 tacaggattc accggcgttg tgccgtcgga tcaattacgc gtagctgccc accgaattgc
      121 ggatattatt tatgaggctt tgacaggaga tatgggcgtc tttagcaccc gcattgcatt
      181 tgtagccaaa acaggtaaca aattcgcgtt acagattgct gattctgatg gatataacgc
      241 gcattcgatc attgaatatt ctgagccaat catttcaccc gcatggtcac ctgatgggag
      301 caagattgct tatgtctctt ttgaaaataa gaagccggtt atttatgtgc aaacgctggc
      361 aacacgtgaa agaaaggccg ttgcaaattt taaaggaagt aatagtgctc ctgcttggtc
      421 accgaatggt gacaagttag cggttgtatt aacgttacac ggtggttcgc agatattttt
      481 aattaatgca gatggtagcg gtctacaacg attagcccaa agtcccggta ttgataccga
      541 gcccaatttc tcacccgatg ggcggtcgat tatttttact tcggatcgcg gtggaagtcc
      601 gcaaatctat cgaatggcta tttcaggtgg taacgccggt gttgcagaac gacttacatt
      661 cgaaggaagt tataatgtaa gtccacgctt tagcccggat ggaaaaagtt ttacttatat
      721 tcatcgcaat ggtgatcggt ttaatattgc cctgcaaggg cttgccaacc gccaagtgca
      781 attattgacg gatagcaagt ttgatgaatc tcccagcttc gctcccaacg gaaaaatgat
      841 tttgtatgct acagaaataa gtggacgtgg tatattatcc gtcgtttcca gcgatggaca
      901 aacgaaacaa cggctttcat ctcaaacggg tgatattaga gaaccgtcgt ggggtccttt
      961 gctgaaatca cagcgaaata atcacttcat taactaattt gaaaaggata agcaatgaaa
     1021 aaattattaa cagtgctgat tatctgcttg ttgtcggcat gtgcaagtca aaataaacag
     1081 tcagctgaag ttgatgatcg tgcaatggca ggtggtggtt ccggaagtca agatggagca
     1141 ggtgcaggtg gatttggttt aagccctttg cgtgatccta ataatattct atctcagcga
     1201 agtgtttatt atgactatga tagctatacc ataaaaagcg aatatagaga gctcgtgtta
     1261 gcacatgcac gttatctccg tgataatggc gatgcaaaag tcttattgca agggaacacc
     1321 gatgatcggg gtagtcgtga atataatctt gcgctggggc aaaaacgtgc taatagcgtg
     1381 aagaacgcga tgttgttatc tggagcacga gatcaacaaa tagaagcagt aagtttgggt
     1441 gaagagaaac cccgtgcaac aggacatgga gaatctgctt gggctgagaa tcgtagagcg
     1501 gatattctct accaaggaga gtattaatgg tattgcgcgc ttcttctttt ctggttgttg
     1561 cgcttttatt ggttagtaag cccagtattg ctgggctttt tggcgatgaa aaagcgcgtg
     1621 agcagatcga tcttttgcag aaacaaacac aagcaatgga agcgcgcatt gcgggaatgg
     1681 aggaattgct caaaagtaat gcattaattg agctttattc tcaagtggaa ggttttaaaa
     1741 tagagctagg aaaattgact ggccagatcg aggtattgac tgaagaaaac aagttacttc
     1801 gtaaacagca gaaagatttc tatctcgatt tggataatcg cttgaatcag gttgagtcgg
     1861 aaggatcaag agaatcaggg aatacatcgc tgggtgaacc tgattcatca actgcattgc
     1921 catccgaaat tgaaatgact gcacaaacag tcgatggctc ggaacgcgaa gcttaccaaa
     1981 ctggatataa tttattccga gaaggggatt atttagcagc gatcagtcat tttgagcaat
     2041 ttctcaaaca atatgcaagt tctagtcttg cgccaggagc ggcttactgg ataggaaatg
     2101 cccactacgc actgcgtaac tttcaagaag ctatttctgc tcagcaacgt gtaatcgatc
     2161 aatatcctaa gagcgcgaag gcgcctgacg cacttctcaa tattgctagt agtcaattgg
     2221 aaattcctga cgtatcggct gctaaaaaaa ccttagaaaa ccttatccaa aaatatccca
     2281 atagtgaggc tgctggtaaa gcaaaaaagc gcctaactag cctgaaataa tttttatcaa
     2341 gaaggaagtt tttacagctt cttgcttttt tctccatgga aaagttgcga gttagtgaga
     2401 ttttttactc cctgcagggt gaaacaacgc gtatgggttt gccgacggta ttcattcgtt
     2461 taactggctg tcctttacgc tgtgtttact gtgatacaca gtatgcattt accgggggaa
     2521 agatgatgtc gattccggag atattggata aggtttcaaa tagtaaggca cgctatgtaa
     2581 cagttaccgg tggtgaaccg ttggcacaaa aagcctgttt atctttactt actgcattat
     2641 gtgatgccca ttattctgtc tccattgaga ccagtggggc attcgatatt agtgaaattg
     2701 atccgcgtgt ctcccgcatt gtcgatatta aaacgcctgg atcgggcgaa gtatcaaaga
     2761 atctttttga gaacctagcg ctgatatcga gtaaagacga aattaaattt gtattgtgcg
     2821 atagggaaga ttatcaatgg gcgctacaga taatgaatga ctatcaatta gctcaagtat
     2881 gccccatctt attttcccct acttatggtc agctagcggc tgatttatta gcaaaatgga
     2941 tcttggaaga tcatgcaccg gttcgattgc agctccaatt acataaaatt ttgtggggag
     3001 aggtagcggg tcgatagccg aatgggttaa ttttctggct actttgtttt attgagatga
     3061 aaaaagcggt tgtattggta tcgggaggac tcgattctgc aacgactctt gcggttgctc
     3121 gtgatcaaca tttcgattgt tatgcactca gtattgatta cggacagaga agtcgctctg
     3181 aactcgatgc agcgttaaat gttgggaaaa tgatcggtgt cacggatcat caagtcatta
     3241 gcgttgatct gcgaaacttg ggaagatcag cactgacgga tcaatcgatc gatgttccca
     3301 cgacaggaat gcaagtgggt attccagtga cttatgtgcc agcacgcaat acgattatgc
     3361 tttcgttagc gttagcttgg gctgaaagcc tagcaagcca agctattttt attggcgtca
     3421 atgcaatcga ttattctggc tatccggact gtaggccgga ctatatcgag gcttttgagc
     3481 gtatggcaaa tctagccact aaagctgcat atgagggggt aaaactcaag attcatgcac
     3541 cactgattga tttatcgaag cgcgaaatta ttttgttggg tactcggttg aatgttgatt
     3601 atagtcgtac agtttcctgt taccaagcgg atgagcatgg attggcatgc ggtgtatgtg
     3661 atgcgtgccg gttgcgtaag atgggttttt cagcagctgg catttctgat ccaactcgat
     3721 attcaccaat ctatcgagag ccccagtaat caaggaaaag ttatcaattc tcatgctgat
     3781 gagaatcgaa ctcacgcacg atgctgacac cgagcttttc tataccttta ataattccaa
     3841 gttttgcaac acttacttta acttgaggtg atttaaactc ggccaagata agttgggcga
     3901 tatgctctgc caaagcttcg agtagcatga aatgcttgtc agacaaagtg tgcttgattc
     3961 tgtcagcaat aagggaataa tctatcgtat ctttgatatc atcactggca ctcgcgctac
     4021 gtgtgggaag tgcaatttct aagttaagtt cgatgagttg cgcgactttt ttttcccaag
     4081 gataaagacc gatcatcgct ttggttcgaa aattttgtaa gaaaatgaca tccatgggag
     4141 taatttactg cattatattt acaatagtgc tagatttttt tgttaacgat tctaatcgaa
     4201 ttagagaggc gaagctattt tggatatagc aacgatcatt ttattactga tggcctattt
     4261 acttggatcg ataccatttg cgatggtagc cagtaaatta ttcgacttac ctgacccaag
     4321 aacctacgga tcaaaaaacc caggtgcaac caatgtgtta cgaagtggca aaattgctgc
     4381 tgcagttgta accctattgg gggatgccgg taaaggctgg ttagcagttg agctaactcg
     4441 agtttatgtt tcgatggaag gaggcggtta cgaattaatt agcggtgctg cattattggt
     4501 ttttctcggg catctattcc ccatttttct caattttaaa ggcgggaaag gtgtagcgac
     4561 agctgcaggc gtattacttg ggtttaatgt gttattaggt tcgcttgtga ttttgacttg
     4621 ggttatcgtt gctgctatct caaggctatc gtccctatct gcgctcactg ctgcggtgct
     4681 tgccccgatt tatagttatt taatttttaa ttgggatacc cctgtttggg cggttatcat
     4741 catttcgtta ctgctaatgc tgcgtcatca agcaaatatc attaatttga ttgaaggaaa
     4801 ggaaacgcgt attggtaaaa aagcaacaga caagacttaa gtatgctgaa tttcaaagaa
     4861 tccaagtttc gtaaaataaa gacacgaaat gggggacatg acgaaacgat tatcgccgac
     4921 gaagtataac caagaatcca gtgagcgact agttaagttt ttttaaatta aatcgattgg
     4981 cgcgagaggg gtaaccgcaa atatataata agatattgat ttaaatatta ttaatattta
     5041 ttgattttat aagtaccgcc aaaaatgccg tcaatttatt ttgctgggct ggttcctagc
     5101 tgtcaacatg cttggtttgg tgttacagct cgcatgattt aataaactca ttaagcgcgc
     5161 gatctacttc atcacgtaga tacatttcaa tctttgctgg atcttcaata ttcacaagat
     5221 taggtgcaga ttgtttgcaa acagagtgca atgtgtcgcg aagcttgcga cctgcttcga
     5281 atgctgcatt gtgtacgtca gaacgcaata ccaaattacg tcgcgcaagg tctaattcta
     5341 gctgctcttt atcagcacga accttgtcaa gtctagccct ttcgtaagag ccattaatat
     5401 taagtttaag ttcaggattt ttcatgtgta tagaatttga atttctagat ttaagatgag
     5461 gccttcatta cccccactta aattatcaag agaagaacct actttttatt tagttactca
     5521 cttacacgcc tattggtatt aaaagttact gattcggtaa tcgtgaccgg tttagtaacc
     5581 tctaccattt agaatggtca gaaaacgaaa cgctatacac aggatagcgt tttatgtttt
     5641 aaaggcagaa acgctatata gtgttcaatt caatttcctc tcctttgcct ttggaagtca
     5701 taatctgtac gaatgatctc ccggtatatt tggcaagctt ttgttcaatt cccaataatt
     5761 gcgcaaaggg tagcggatta tcatgaatcg catcgcgcac acacgaccag ataagtgatt
     5821 tcatcactgg atcaatttct aatttagcca gaacctgtct ttcaagtgat tgcttagctt
     5881 tatttgtgag tcgtttagtt tcgtttctgc ttagtccgta atacctggca acttcggcat
     5941 gaaacagatt aatcaaacgc attagattac ctttgccgcc catgattttg tttccctgca
     6001 ttttgttgtc taccaagggt aaaattaaag catgtgcatg gggtgcggtt tcatccaggt
     6061 gaacatcgaa tgacaacaac acccccggaa tatttcttac tatccaatcc atgcaacatt
     6121 ggaagaatgg ccgagtgtct tgttggtgtt gatcaatggg tagactaaag atggcttcta
     6181 ctgccatcac ttggttttta cgcggctgat ctatgccagc ctgcaccatc aatacttttg
     6241 catggcgatc aatcgcttct gcgttatccg gtttagtcag tgagtaattc aatggtgttc
     6301 taaatggatt aatattcgcg ctagcacctc gttcagcttg tagcgctcgc ttattgtgct
     6361 tcaaagcgat cagaacgcca tttttacctt tcaccgtgcc catgcggaat aggtgactac
     6421 cggccatcgt ttaaccatcc tccagccgca acagataccg ataaactgcc cacaattcga
     6481 tcagactcat tgaatcaata tcagcaccaa taccaagatg agaaaaccgt attaacagca
     6541 tttcaagttg ttccaaccaa gtatcaggat tcatggctgt caccgttgcg tagcaaataa
     6601 catccaacgc ggtgtttatc gccattttcc gcttcaatga cttgccaaat tgtgtcaatg
     6661 caaaagccat tttttcgaag ctgcaatatt cgtgcgggcg ggtggtagat tcccaatcca
     6721 cggctagctt caaaagtgtt gatgttaaac aaccgcaacg cttgtaatag ccgcgcgcat
     6781 tgctgtttgg catcatctcc ggtaaactgc tgattgattt ctttgaaggc cgcttcttta
     6841 tggggcggta tttttttgtc catgttatgc cccctgat
//
LOCUS       DAOQKQ010000139         6875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_5483_length_6875_cov_5.233724, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000139 DAOQKQ010000000
VERSION     DAOQKQ010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 6875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6875
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5483_length_6875_cov_5.233724"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="PKZ49_09375"
     CDS             complement(<1..539)
                     /locus_tag="PKZ49_09375"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HND36812.1"
                     /translation="MKSINVGLLGIGTVGGGTFTVLKRNQEEIARRVGCNIVIQMVAD
                     KDIEKARHIVGSQTIVTQNAQEIVANPDIEIIIELIGGQTIAKELILQAINNGKHIVT
                     ANKALLANHGSEIFSAAQQKGVIVAFEAAVAGGIPIIKALREGLTANRIEWIAGIING
                     TSNYILSEMREKGLAFSTAL"
     gene            complement(635..1861)
                     /locus_tag="PKZ49_09380"
     CDS             complement(635..1861)
                     /locus_tag="PKZ49_09380"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HND36813.1"
                     /translation="MQPIKKSSKLENVCYDIRGPVLDRARQMEEEGYHIIKLNIGNPA
                     SFGFEAPEEILQDVIRNLSAASGYCDSKGLFAARKAIMHYTQEKQIANVQMEDIYVGN
                     GVSELIMLTMQALLENNDEVLIPAPDYPLWTAAVSLAGGTARHYVCDEQSDWFPDLDD
                     IRTKITDRTRAIVIINPNNPTGSLYPKELLLEIIEIARQNRLIVYADEIYDKILYEQA
                     THTSVASLADDVLFITFNGLSKNYRAAGFRSGWAVVSGNKHDAQDYIAGLNMLASMRL
                     CANVPAQFGIQTALGGYQSIHDLTMPTGRLKRQRDLAWQMLTDIPGVSCYKPQSALYL
                     FPHLDPKIYPIQDDERFVLDLLLEEKVLVVQGTGFNWLHPDHFRVVFLPNSDDLTEAI
                     SRIARFLDRFRKRYKT"
     gene            2054..2422
                     /locus_tag="PKZ49_09385"
     CDS             2054..2422
                     /locus_tag="PKZ49_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HND36814.1"
                     /translation="MKFHLSNIEGINTFSGYGAGYVVINQVRYDRNLVVFPDRIIENW
                     CIRNSGRFAIDDFDEILTYQPEIILLGTGETQRFPSPQLIKAMLNARIGLEVMDTFSA
                     CRTYNILSTEGRRVAAALLV"
     gene            2488..2772
                     /locus_tag="PKZ49_09390"
     CDS             2488..2772
                     /locus_tag="PKZ49_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36815.1"
                     /translation="MGALVFYTFVFFIGYFVIHGFNLLIGRKLLNRKITALVIVCLIA
                     LMHGYKILTSPLPAQQDADAFQALGYYVMFPILVIVTACVFFGLQDKEDE"
     gene            2870..3952
                     /gene="hisC"
                     /locus_tag="PKZ49_09395"
     CDS             2870..3952
                     /gene="hisC"
                     /locus_tag="PKZ49_09395"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HND36816.1"
                     /translation="MTHLLRNVIRPEIFELSAYHVPPAKGMIKLDAMENPYTLPLTLR
                     AEIAQLAENATINRYPDPTASVLKSILRKTLSVPDDMSILLGNGSDEIIQMIALAAAK
                     PGAVLLGVDPGFAMFRIIATFSGMSYVGVPLNEDFSLNLEAMRTAITRYQPAIIFLAY
                     PNNPTGNLFDIQVIHQIIQEAPGIVIIDEAYHAFAGVSFMDQLAQFPNLLLMRTLSKL
                     GLAGLRLGLLIGRSEWLEQFEKLRLPYNIGVMTQLIAEKVLQHYEDLLKQTAAIKTER
                     SKLLSELSGLSGITTFPSEANFILFRIKRASEVYALLKQKGVLIKNLDGAHPLLKDCL
                     RVTVGTPHENDQFYHALLQVLEDTNC"
     gene            3991..4578
                     /gene="hisB"
                     /locus_tag="PKZ49_09400"
     CDS             3991..4578
                     /gene="hisB"
                     /locus_tag="PKZ49_09400"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HND36817.1"
                     /translation="MRQIKVTRNTLETQISVEVNLDGSGHSKLNTGIPFLDHMLDQIA
                     RHGMIDITVEAKGDLHIDAHHTVEDIGITLGQALAKAWGDKKGVRRYGHAYVPLDEAL
                     SRVVLDLSGRPGLGYRVEFVRARIGEFDVDLIHEFFQGLVNHAMITLHIDNLFGDNAH
                     HQAETVFKAFGRALRMAIETDSRAINLIPSTKGSL"
     gene            4610..5260
                     /gene="hisH"
                     /locus_tag="PKZ49_09405"
     CDS             4610..5260
                     /gene="hisH"
                     /locus_tag="PKZ49_09405"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HND36818.1"
                     /translation="MTAIAIVDYGMGNLRSVSKALERVAPYATVQVTSDPKIVDKASH
                     IVVPGQGAMPHCMQELDKFGLKDVVIDAARNKPFLGICIGLQMLFDESEEGNVRGLGV
                     FPGKVVHFSAGGEADVQGGKLKIPHMGWNQVYQTMNHPLWDGIRSDARFYFVHSYYVK
                     SEDPNLSAAYSLYPTPFTCAIAKDNIFAVQFHPEKSHIAGLTLLSNFAKWEPHSSH"
     gene            5322..6068
                     /gene="hisA"
                     /locus_tag="PKZ49_09410"
     CDS             5322..6068
                     /gene="hisA"
                     /locus_tag="PKZ49_09410"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HND36819.1"
                     /translation="MLIIPAIDMKDGQCVRLKQGDMEAATVFSENPDEMALQWLNQGA
                     RRLHLVDLNGAFAGKPKNESVIRSIVKALNGNIPIQLGGGIRDLETIERYLDQGITYV
                     IIGTAAVKIPGFLHDACYAFPGQIMVGLDAKNGKVAVDGWSKVTGHDVVDLAKKFEDY
                     GVEAIIYTDIGRDGMLSGVNIEATVALARELTVPVVASGGISNLEDIKALCKIEAEGV
                     MGAITGRALYEGTLDFKQAQDLADKLSVTA"
     gene            6155..>6875
                     /gene="hisF"
                     /locus_tag="PKZ49_09415"
     CDS             6155..>6875
                     /gene="hisF"
                     /locus_tag="PKZ49_09415"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HND36820.1"
                     /translation="MGLAKRIIPCLDVNNGRVVKGVNFVSLKDAGDPVEIARRYDEQG
                     ADELVFLDITASSDNRDLILHIVEDVASQVFIPLTVGGGVRKVEDVRRLLNAGADKVS
                     INTSAVLNPQLVADAAGYYGAQCIVVAIDAKQVGEQGSQVPRWEVFTHGGRNATGLDA
                     IEWAKKMQALGAGEILLTSMDRDGTRHGFDLALTRTLSDAIDLPVIASGGVGSLNHLV
                     DGILQGHADAVLAASIFHYGQH"
BASE COUNT         1925 a         1261 c         1554 g         2135 t
ORIGIN      
        1 agtgcagtac taaatgctaa tcccttttca cgcatttcgg agagaatata attgctcgtt
       61 ccattgataa tacctgctat ccactcaatt cggttagctg taaggccttc acgcagcgcc
      121 ttgatgattg gaatccctcc agcaaccgca gcttcaaaag cgacaatcac ccctttttgc
      181 tgtgctgctg aaaaaatctc actgccgtga ttagcaagca aggctttgtt tgccgttacg
      241 atgtgcttgc cattattaat ggcttgtagg atgagttcct tggcaatggt ttgaccaccg
      301 ataagctcaa tgataatttc gatatcaggg ttagccacaa tttcttgagc gttttgggta
      361 acaatcgttt ggctaccgac gatatggcgt gccttttcga tatctttgtc tgcaaccatt
      421 tgaataacga tattgcatcc tacacggcgc gcgatttctt cttgatttcg ttttagaaca
      481 gtgaaagtac cgccaccgac cgttccaatt ccgagtaaac caacattaat agatttcatc
      541 ttatattctt aagtaaataa gctaatgatc aatacgctct attttaattc aacccatcca
      601 ggataagcca atttattgaa tgaataggtt gtaattaggt tttatagcgt ttccgaaaac
      661 gatcgagaaa gcgagcgatt cgtgaaatag cttcagtcaa gtcatcgcta ttcggtaaaa
      721 atacaaccct gaagtggtct ggatgtaacc aattgaaacc tgtcccttgt acaactagca
      781 ctttttcttc tagcaataaa tccaagacaa agcgttcatc atcttgaatt gggtaaattt
      841 taggatcaag atggggaaat aaatataagg cagattgtgg cttataacag cttacgcctg
      901 ggatatccgt gagcatttgc caggcaaggt cgcgctgccg cttgagtcta ccggttggca
      961 tcgtcaaatc atggatgctt tggtatccac ccaatgcagt ttggatacca aattgagccg
     1021 gaacatttgc gcataatcgc atcgaagcca acatatttaa gccagctata taatcttgtg
     1081 cgtcgtgttt attgcctgag acgacagccc aaccagaacg gaaacctgcc gcacggtaat
     1141 tttttgaaag accattgaag gtaataaata aaacatcatc ggcgagcgat gcgactgaag
     1201 tatgggtcgc ttgctcatag agtatcttgt cataaatttc atcagcgtac acaattaacc
     1261 ggttctgccg agcaatttcg atgatttcta ataatagttc tttgggatag agcgatcctg
     1321 ttggattatt gggattgata atcacgatgg cgcgcgttct gtctgtaatt ttggtccgga
     1381 tgtcgtctaa atcaggaaac caatcagact gttcatcgca gacataatgg cgagcagttc
     1441 ctcctgcaag cgaaacagca gcggtccata acgggtaatc aggcgcgggt attagaactt
     1501 cgtcgttatt ttccaataaa gcttgcatgg tcaacatgat gagttccgaa acaccattac
     1561 ccacataaat atcttccatc tgcacgttag caatctgttt ttcctgcgtg taatgcatga
     1621 ttgctttgcg cgccgcaaat aatccttttg agtcacaata gccagatgct gccgaaagat
     1681 tacgaataac atcctgtaaa atctcttcag gggcttcaaa tccaaaggaa gctggattgc
     1741 cgatattgag tttaatgatg tgataccctt cttcttccat ttgtctcgca cgatccaaaa
     1801 caggacctcg aatgtcataa catacatttt caagcttgct ggattttttg atcggctgca
     1861 tggtaggtca agtagtaata aagaatatgg tatcgtaagt tgtgaatgga ttggatgagt
     1921 acaaactcaa tgctatgtta cccgacacag ctatgctatt gcaaaatatg tattcgattg
     1981 aatggtggtt taagctggta ttttgccatt atttgatgat gattatgttt tataagtaag
     2041 ggggagggcg taaatgaagt ttcacctttc taatattgaa ggaataaata cgttttcagg
     2101 ttatggggct ggctacgtcg tgatcaatca agtacgctat gatcggaatt tggttgtatt
     2161 tccagatcgt atcattgaaa attggtgcat aagaaactct gggcgattcg ctattgacga
     2221 ttttgatgaa atacttacct atcaacctga gataatattg ctgggtacag gtgaaacgca
     2281 gcgatttcct agtcctcaat taatcaaagc gatgcttaat gcaaggattg gattggaagt
     2341 gatggacaca ttttctgctt gtcgcaccta caatatttta tcaaccgagg gtcgcagagt
     2401 agccgcagcg cttttagtct aacgactttt gtaaaatttt tgacgattac acaaataaat
     2461 tagcttggat aataaggaat accatcaatg ggggcattag ttttttatac ctttgttttt
     2521 tttattgggt attttgtgat tcatggcttt aatttgttaa tcggtcgtaa gttgttaaat
     2581 cggaaaatta ctgcactcgt cattgtttgt ctgattgcac taatgcatgg atataaaata
     2641 ttgacgagtc cactacccgc tcaacaggat gcagatgctt ttcaagcact tggttactat
     2701 gttatgtttc caatcttagt cattgtgacg gcctgtgtgt tttttggatt acaagataag
     2761 gaagatgaat gaataccagc gtgactctgt tttatatctt taatttttga taatcgttag
     2821 cgttatttct cctttttctt tatttattat cttttcttta atttgaaaca tgactcactt
     2881 gctcagaaat gttatacgac cagaaatttt tgaactttct gcgtatcatg ttccaccagc
     2941 taaaggaatg atcaaattag atgcaatgga gaatccttat acattgcctt taacgcttag
     3001 agcggaaatc gctcaattag cagaaaatgc aacgattaat cgatatccag atccgacagc
     3061 gagcgtatta aagagtatct taagaaaaac tttatcagtg cctgacgata tgtcgatcct
     3121 tctgggtaac ggctctgatg aaattattca gatgattgcg ttggctgctg caaaacctgg
     3181 tgcagttcta ttaggggttg atcctgggtt cgcaatgttt cgtatcattg ctactttttc
     3241 tgggatgagt tatgtaggtg ttccgctcaa tgaggatttt tcattaaatc tcgaagcaat
     3301 gcgtacagcg attactcgct atcaacccgc cattattttt cttgcctatc ccaataatcc
     3361 aacaggtaac ctatttgata tacaagtgat acatcagatt attcaggaag cacctggtat
     3421 tgttattatc gatgaagcct atcatgcttt tgctggcgtt agttttatgg atcagttggc
     3481 gcaatttcct aatttactat tgatgcgaac cttatcaaaa ttgggtcttg cagggttaag
     3541 attagggttg ctgattggaa ggtctgaatg gcttgagcaa tttgagaaac tgcgtttacc
     3601 ctataatatt ggcgtcatga cccaattaat tgctgaaaag gtattgcagc attatgaaga
     3661 tttattaaag caaacggcag ccatcaaaac agagagaagt aagctattat cagaacttag
     3721 tggtttaagc ggaataacga cttttccttc agaagctaat tttattttat ttcgtattaa
     3781 gagagcgagt gaagtgtatg cgttactcaa gcagaagggt gttctcatta aaaatctgga
     3841 tggagctcat cccttattaa aggattgttt acgtgttacg gtgggcacac ctcatgaaaa
     3901 tgatcaattc tatcacgcgc ttttacaagt acttgaagac acaaattgtt agattgatat
     3961 gtatcgtaag gcttaactga taatattttt atgcgccaga tcaaagttac ccggaatacg
     4021 ttggaaacgc agataagcgt tgaggttaat ttggacggaa gtggtcattc aaagttgaat
     4081 actggtatac catttctaga tcatatgtta gatcagattg ctcgtcatgg gatgattgac
     4141 atcacagtgg aagcaaaagg agatttgcat atcgatgcac accacacggt tgaagatatt
     4201 ggaattacac ttggtcaagc attagcaaaa gcttggggtg ataaaaaagg ggtgcgtcgt
     4261 tatggtcatg cctatgttcc actcgatgaa gcgctttcta gagtcgtgct ggatttatca
     4321 ggtcgaccag ggcttggtta tcgggtcgaa tttgttcgtg ctcgcattgg tgagtttgat
     4381 gtcgatttaa ttcacgaatt ttttcaagga ttggttaatc atgctatgat tacactccat
     4441 attgataatc tttttggaga taatgcgcac catcaagctg aaaccgtttt taaggctttt
     4501 ggaagagcat tacgtatggc aattgagact gattcccgcg caattaatct aatcccatcc
     4561 acaaaaggtt cattgtaagc gctactcgta accgcattaa ttattaagta tgacagctat
     4621 tgcgattgtt gattacggaa tgggtaattt acgttcagta tccaaggccc ttgaacgagt
     4681 tgctccctac gcgacagttc aagttacgag tgacccaaag attgtagata aagcttcgca
     4741 tatcgttgtt cccggccaag gcgcaatgcc gcattgtatg caagagttag ataaatttgg
     4801 tctaaaagac gttgtaattg atgctgccag gaacaagcca tttctaggta tttgtattgg
     4861 tttgcaaatg ctgtttgatg aaagtgaaga aggtaatgtg agaggcttgg gtgtcttccc
     4921 agggaaagtt gtgcacttct cggctggtgg cgaagctgat gttcagggcg gtaagttaaa
     4981 aataccccat atgggctgga atcaagttta tcaaacaatg aatcacccgc tatgggatgg
     5041 aattagaagc gatgctcgct tttactttgt tcatagttat tatgtgaagt cagaggatcc
     5101 aaacttatca gcagcctact cgctttatcc taccccattt acatgcgcga tcgctaaaga
     5161 taatattttt gcagtacaat ttcatccaga gaaaagtcac attgctgggt taacattatt
     5221 aagtaatttt gctaaatggg aacctcattc cagtcattag tacaattcaa ttcatcgttt
     5281 cattccgttt ttttattatc aattatttat cctaacaaca tatgttgatt attcctgcga
     5341 tagacatgaa agatggtcaa tgcgtgcgac taaaacaggg tgatatggaa gccgcaactg
     5401 ttttttctga aaaccctgat gaaatggcac tacaatggtt gaaccaaggt gcgcgtcgct
     5461 tacatttggt ggatttgaat ggagcttttg ctggtaaacc gaaaaatgag tctgttattc
     5521 gctcaatcgt taaagcgctt aatggcaata tcccaattca attaggtgga ggaattcgtg
     5581 acttagaaac gattgaacgc tatctggatc agggaattac gtatgtaatc attgggactg
     5641 ccgcagtaaa aattccaggt tttttgcatg atgcttgtta tgcatttcct ggtcaaatta
     5701 tggttggcct tgatgcgaag aatggtaaag tagctgttga tggctggtcg aaagtgacgg
     5761 ggcatgatgt tgtcgattta gccaagaaat ttgaagacta tggcgtagaa gcgattattt
     5821 ataccgatat tgggcgagat ggaatgctta gcggggttaa tattgaagca accgttgcgc
     5881 tagctcggga attaaccgtt ccggtagtag cgagtggagg catttctaat ttagaagata
     5941 tcaaagcgct ttgcaaaatc gaagcagaag gtgtaatggg tgcaatcacc ggtagagcct
     6001 tatatgaggg tacgctggat ttcaaacagg ctcaagactt ggctgataaa ttaagtgtaa
     6061 cagcctaaat taagtgtgca tcctgtgatc tgatcattac tttactattc acttttttct
     6121 ctctaatatt tttctttact aaggttttta ggcaatgggt cttgccaagc ggattattcc
     6181 ctgtcttgat gttaacaatg gacgtgttgt taaaggggtc aattttgtat cgttaaaaga
     6241 tgcgggtgat cccgttgaga tcgcacggcg gtatgatgaa cagggagctg atgagcttgt
     6301 gtttttagat ataacggcaa gttcggataa tcgggacttg atcctgcata tcgtggaaga
     6361 tgttgcgtca caagtcttta taccgttgac ggtagggggt ggcgtacgta aagtagagga
     6421 tgtgcgaaga ttactgaatg ccggcgctga taaagtcagt attaacactt cggcagtact
     6481 aaatccacaa ctcgtagcgg atgcggcggg ctattatggt gctcagtgta tcgtggttgc
     6541 catagatgca aaacaagttg gtgagcaagg tagccaggtt ccgcgttggg aagtatttac
     6601 ccatggcggc cgaaatgcta ctgggcttga tgcgattgag tgggctaaaa agatgcaagc
     6661 gttgggtgcg ggtgaaatat tgctgacaag catggatcga gatggtaccc gacatggatt
     6721 cgatcttgct ttaacacgaa cgctttccga tgcaatcgat ttgcctgtaa ttgcaagtgg
     6781 cggggtagga agcttgaatc acctggtaga tggaattctt caagggcatg ctgacgcggt
     6841 tcttgcagca agtatctttc attatggcca gcata
//
LOCUS       DAOQKQ010000140         6865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_5498_length_6865_cov_5.650073, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000140 DAOQKQ010000000
VERSION     DAOQKQ010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 6865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6865
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5498_length_6865_cov_5.650073"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2867)
                     /locus_tag="PKZ49_09420"
     CDS             complement(<1..2867)
                     /locus_tag="PKZ49_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HND36821.1"
                     /translation="MTLRQPIADYEKRLRLLDSQRSFLVQAPAGSGKTGLLIQRFLVL
                     LTKVDTPEEIIAITFTRKAAQEMRQRILQALEQGQSDIVPEDCYVQQTWELAREALRC
                     DRNKDWQLLNNPNRLRIQTIDSLCTMLVGQMPVLSQLGALPRITEDATDLYQEAARRT
                     IEAIESGHEWSDAIAHLIILFDNQLRRLQQLIADMLRRRDQWLPNLVESSHEPEVRQH
                     LEKALTELVEVALQKLVDQAQAINQNEIITLANFAAAHVPNDNPISRCLSLSTLPGSS
                     CRDDRAIWEGIAALLLTQEGNWRKQLTKNEGFPADKESKNKQEKETYSTMKQRMKALL
                     QDLKSAEGFRNQLVQFRALPASRYADSEWETLQALFKLLKLAAAHLEILFQQTGQIDF
                     PALTLAASQALGKPEAPTDLALALDYRIQHLLVDEFQDTSLNQAELLMRLIAGWQVGD
                     GRTLFLVGDPMQSIYRFRQAEVGLFLEIRDRGLFGQIPIEFVRLSVNFRSQQGVIDWI
                     NQHFPNILPNQDEVAMGAIAYTPSIAIHPKMEEQGVTFHPYWQKDEKAEAQQVVSIIQ
                     KTKQADPTQTIAVLVRNRAHLAQIIVQLRQQGLRYQAVEIERLSHRPVIQDLLALTRA
                     LLHQGDRIAWLAILRAPWCGLTLQDMLILSEPVDQVLLDSLRNSTTLARLTEDGQQRL
                     ARVVPIIEGALSQLGKQTISRIVEGAWLALGGPACVMDEIDLQDAAIFFQLLQQLETR
                     GVADALYDSETLQEKVAALYALPNLSEDAAMIQLMTMHKAKGLEFDVVILPGLGYSSK
                     QDDPQLLYWMKHHFQSGQTSLLFAPIDALGDKPNSTTAYLKQITQKKARYETSRLLYV
                     AATRAKHQLHLLGHINHQEDTPDAEMKVPTKDSLLASLWPAVSHQFQITGESLANKST
                     RIANINPNNDFLLQPIQRFRLVKSWQLPTPPQKYRSQAFHFS"
     gene            complement(2864..5587)
                     /locus_tag="PKZ49_09425"
     CDS             complement(2864..5587)
                     /locus_tag="PKZ49_09425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HND36822.1"
                     /translation="MKQDWLNPKQININELLKDSNQNTIVLTGNQRLTGVLRGLSEQI
                     FISQQFSVWKSPFIIPWNSWLLMLWEESLISGLLPKYEQLLSPWQEYVIWQEIIKADL
                     SVKPTHSTIQLVQNAWQLVGSWCIPQNESAFSYNQDSQLFWKWLLRFQSICKEKNFVS
                     VVSIANKLQPLFKSQKLISPFYLILTGFDELNPLQVQFLTTLENLGCRIEWVERQTKS
                     NQVFRIACKDVHEEREWMARWVRKKQEDNPGARIGIVVPQLQDQRDAIQHTLDEILIP
                     EIFHPESQHLTPPYNFSLGKALNQFPPITTALNLLNFFGTTLRFETVSHLLRSPLIAG
                     WAQESGARALLDAKLREIGEPVFTVDALIDFCVRFDSNTACPIFLQQLQSTKKWVTVN
                     YNNAKPGQWSSRIDHFLKRMGWLEDYSLSHETFQLIGAWRELLAEFASLDWVMGEMTA
                     IDAISLLSQMASQRIFQSQVNNQAIQILGPLEAQGIEFDFLWVAGLHDSAWPNHFDPN
                     PFIPIAIQRKHQIPHSSGQRELMVTTQQMQRILGSANEVIVSYPQFDEESILRPSPLI
                     KGFTAIDAQDLSLSNVPLWRDIIYAQSQSEIIQQETTLPVETTQVRGGSRIFELQAAC
                     PFHAFAELRLHASPLGKVQMGLNAMERGNLVHRAMEKIWGALNSHQQLMALSEAQLAE
                     LIRTQVMLTIDEIRVDYPYTLTNRIRMIEIARISAQIDKWLALEKTRSPFAVTSREQT
                     VEIELAELKIKLKIDRIDTLVDGRQIIIDYKTGKVSPSEWFGDRPDKPQLPLYSLVLA
                     KQQLAGITFAQLRVDQIAFSGITVEENLLPKVKSFEKMTQIQEIADWPTVIMNWQRVL
                     ENLALQFLSGEATVNPKKYPETCQHCSLQVLCRINEATILSDIEFNPETEA"
     gene            5744..6199
                     /locus_tag="PKZ49_09430"
     CDS             5744..6199
                     /locus_tag="PKZ49_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36823.1"
                     /translation="MNNNFIKTVLFIFIVSWSNLAISEQAESAAQVAETQEKADDSEI
                     FGQGWKLVRSPEMGNSGKFVHMVLVDLDKEMDKTVYSAAINRLCGKATDFCRIRFWTQ
                     ERFIPEKVTPTPEQLKAQKADYLFNPTAGVKKTTWSCKVDHDKSHCVNY"
     gene            complement(6324..6767)
                     /locus_tag="PKZ49_09435"
     CDS             complement(6324..6767)
                     /locus_tag="PKZ49_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36824.1"
                     /translation="MNKQKYVIRQQDQNAAVEFLDKQISSGLYWLGEDASQGIVAERE
                     YQDAKANPNDLNAWCEKWLHETQWAQLKNAISIARDQIEKQKYRPQLKTVSLTHNAWE
                     ILSELAKNEGLTLSELIVNRLGNEWLNSYEQNTLPNHHFIKSRQM"
BASE COUNT         1870 a         1516 c         1424 g         2055 t
ORIGIN      
        1 gaaaaatgga acgcttgtga cctgtatttt tgcggggggg tcggtaactg ccagcttttt
       61 accaatcgga accgttgtat gggttgtagg agaaaatcat tattagggtt gatatttgct
      121 atacgcgtcg atttattcgc taatgattca ccggtaattt gaaattgatg actcaccgca
      181 ggccataagc tcgctaataa gctatcttta gtaggcacct tcatttcagc atcaggggta
      241 tcctcctgat gattgatatg ccctaataaa tgtagttggt gtttggcacg cgttgcagcc
      301 acataaagca agcgactggt ctcgtagcgc gcctttttct gggtaatttg ctttaagtag
      361 gcggtcgttg agttaggttt atcgccaagt gcatcgatcg gcgcaaataa taatgatgtc
      421 tgccctgatt gaaaatgatg cttcatccaa tataacaatt gcgggtcgtc ttgtttactg
      481 gaatacccca aaccaggcag aatgactaca tcaaactcca agcctttcgc tttatgcatg
      541 gtcatcagtt gaatcatcgc agcatcctcc gatagattcg gtaaagcata taaggcagct
      601 actttttctt gcaaagtctc gctgtcgtac agcgcgtctg ccactcccct tgtctcaagt
      661 tgttgcaata gctgaaaaaa aatagccgca tcttgcaaat caatttcatc catcacgcaa
      721 gctggtccac ccagtgctaa ccaggcacct tccacaattc gactgatcgt ttgcttaccc
      781 aactggctca aggcaccttc aatgatcggc acaacacgag ctagccgctg ctgcccatcc
      841 tctgttaatc gcgctaaggt cgttgaattt ctgagcgaat caagcaatac ttgatcaacg
      901 ggctctgaca gaatcagcat atcttgcaac gtcaatccac accaaggtgc tcgcagaatc
      961 gcaagccaag cgatacggtc tccttgatgc aatagggcac gtgtcaatgc aagtaaatcc
     1021 tgaatgacgg gacggtggga taagcgttca atttcgacag cctgatagcg taacccttgc
     1081 tgccgcagtt gcacgatgat ttgtgccaga tgtgctcggt tgcgcactaa tacggcaatc
     1141 gtttgggttg gatccgcctg tttggttttt tgaataatcg aaacgacctg ttgtgcttct
     1201 gctttttcat ctttttgcca ataagggtga aaagttacgc cctgctcctc catcttcgga
     1261 tgtatcgcaa ttgaaggtgt ataagcgatg gcacccattg ccacctcatc ttgattgggt
     1321 aaaatattcg gaaagtgctg attgatccaa tcaataacac cttgttgaga tcgaaaatta
     1381 accgataaac gcacgaattc aataggaatc tgaccgaata atcctcggtc ccgaatttct
     1441 agaaacaaac caacttcggc ttggcgaaac cgataaatcg actgcatcgg atcgcctact
     1501 agaaacaaag ttcttccatc ccccacttgc caaccggcaa ttaatcgcat caataattca
     1561 gcttgattga gtgaagtatc ttgaaactca tcgaccaaca gatgctggat acgataatcc
     1621 aatgcaagcg caagatcggt cggcgcttca ggtttaccaa gtgcctgact tgctgccaaa
     1681 gtcagggcag gaaaatcgat ttgccccgtt tgttgaaaca gtatttccag atgggccgca
     1741 gccagcttga gtaatttaaa taatgcctgc aatgtttccc attcagaatc tgcgtagcgt
     1801 gaagcaggta aagcacgaaa ctgtactaat tgatttctaa agccttctgc agattttaaa
     1861 tcttgcaaca acgctttcat gcgttgcttc atagtgctgt aagtttcttt ttcttgttta
     1921 ttctttgatt ctttgtccgc tgggaatcct tcgtttttag tgagttgttt acgccaatta
     1981 ccctcctgag tcagcaagag cgcagcaatt ccttcccata ttgcacgatc atctctgcag
     2041 cttgaccctg gcaacgtcga aagcgataga caacgcgaaa taggattatc attgggaaca
     2101 tgtgctgcag caaaattagc taaggtgatg atttcatttt gattaatcgc ttgtgcttga
     2161 tcgactaatt tttgtaatgc aacttccacc aattcggtaa gggctttttc tagatgttgc
     2221 cgtacttccg gttcatggga agattcgaca aggttgggca accattgatc gcgccgccgc
     2281 agcatatctg caatcagttg ctgcaagcgc ctcaattgat tatcgaataa gatgatcaaa
     2341 tgagcaatgg catcggacca ttcatgaccg gattctatgg cttctatcgt acgtcttgct
     2401 gcttcttgat ataaatcagt agcgtcttca gtaatacgag gtaaagcgcc taactgtgat
     2461 aatactggca tttgaccgac cagcattgtg caaagtgaat caatcgtttg aattcgaagt
     2521 cggttcggat tgtttaaaag ctgccagtcc ttgtttcgat cacagcgtag cgcttctcga
     2581 gcaagctccc aggtttgctg tacatagcaa tcttcaggca ctatatccga ttgcccctgc
     2641 tcaagcgcct gtaagattct ttgacgcatt tcctgtgcag ctttgcgcgt aaaagtaatt
     2701 gcaatgattt cttctggcgt atcaactttc gtcaataaca ctagaaatcg ctggattagt
     2761 aatccggttt taccagagcc tgcaggcgct tgcaccagaa acgaccgctg agaatccaac
     2821 aatcgcagac gcttctcata atcggcaatc ggttggcgca atgtcatgct tcagtttccg
     2881 gattgaattc gatatcgctc aatatcgtgg cttcattgat acgacacaac acctgtaatg
     2941 aacaatgctg gcaagtttca gggtattttt tcgggtttac agttgcttct cccgataaaa
     3001 actgcagtgc aagattctcc aaaacccgct gccaattcat gatgactgtg ggccaatccg
     3061 ctatttcttg tatttgtgtc attttttcaa atgatttcac tttcggtaac agattttctt
     3121 caaccgtgat gccactgaat gcgatttgat cgactcgcaa ctgcgcaaaa gtgatccctg
     3181 caagttgctg ttttgccagc acaagactat acagcggtaa ttgcggctta tcgggacgat
     3241 ctccaaacca ctcgctcggg cttacttttc ccgttttata atcgatgatg atttggcgac
     3301 catcgactaa tgtatcgata cgatcgattt tcaatttgat tttgagttca gctaactcaa
     3361 tctcgacggt ttgctctcga ctcgtcacag caaacggact acgtgtcttt tccaatgcta
     3421 accacttgtc aatttgtgcg ctgattcgtg ctatctcaat catgcggatg cgattcgtga
     3481 gcgtgtatgg ataatcaacc ctaatctcat cgatggttag cattacttgt gttcgtatca
     3541 attctgcaag ctgagcctct gacagagcca ttaattgttg atgtgagttg agtgcccccc
     3601 agatcttttc catcgctcga tgcactagat tgccgcgctc cattgcgttt agccccattt
     3661 gcaccttgcc tagcggagag gcatggaggc gtaattcggc aaaagcgtga aatgggcaag
     3721 cagcctgcaa ttcaaagata cgacttcccc ctctgacctg tgtcgtttct acaggaagag
     3781 tcgtttcttg ctgaataatt tcactttgac tttgggcata aatgatatct cgccataaag
     3841 gaacattact caacgataga tcttgagcat ctatggcagt aaaccctttg ataagagggc
     3901 tagggcgtaa tatgctttct tcgtcgaatt gagggtaact cacgattacc tcgttcgcac
     3961 tgcctaagat acgttgcatt tgctgcgtcg taaccattaa ctcacgctgc ccgctggaat
     4021 gcggtatttg gtgtttgcgc tgtatcgcga tgggaataaa cgggttagga tcaaaatgat
     4081 ttggccatgc gctatcatgt aatcctgcca cccaaagaaa atcaaattca atgccctgcg
     4141 cctctaatgg ccctagaatt tgaatggctt gattgttcac ttgggattgg aagattcgct
     4201 ggctcgccat ttgactcaag agactaatcg catcaattgc agtcatttca cccatgaccc
     4261 agtctaatga agcaaattca gcgagtaatt ctctccaggc gccaattaat tggaacgtct
     4321 catgagacaa tgaataatcc tcaagccagc ccatcctttt gagaaaatga tcaatacgcg
     4381 atgaccactg cccgggcttt gcattgttat aattcactgt tacccatttt tttgtcgatt
     4441 gcaactgctg caaaaaaatg gggcaagctg tattgctatc gaatcgtacg cagaaatcaa
     4501 tcaatgcatc aacggtaaat acaggttcac caatttctcg tagcttggca tccaatagtg
     4561 ctcgcgcacc cgactcttgc gcccatcctg cgataagcgg agaacgaagt aaatggctga
     4621 ccgtttcaaa gcgtagcgtt gtgccaaaaa aattaagtaa attcagtgcg gtcgtaatcg
     4681 gtggaaattg gttaagcgct tttcccaagg aaaaattata agggggcgtc aaatgctggc
     4741 tttcagggtg aaaaatctcc ggtattaaaa tttcatccag cgtatgctga atcgcatcgc
     4801 gttggtcttg taattgcggt acgacaatac caattcgcgc tccaggatta tcttcttgtt
     4861 ttttgcgtac ccagcgcgcc atccattccc tttcttcatg gacatctttg caagcaattc
     4921 gaaaaacttg attcgattta gtttggcgct ctacccactc aatccgacaa cctaaatttt
     4981 ctagggtcgt taaaaactgc acctgcagtg gatttaattc atcgaaacct gtcaatataa
     5041 gataaaacgg tgatattaat ttctgagatt tgaaaagtgg ctgaagtttg ttcgcaatac
     5101 tgacaaccga aacaaaattc ttttccttac agatagattg gaaacgtaat aaccatttcc
     5161 aaaacaattg actgtcctgg ttatagctaa acgctgattc attttgagga atacaccatg
     5221 atcccaccaa ttgccatgca ttttgaacca gttgaatagt gctatgagta ggtttaaccg
     5281 ataaatctgc tttgatgatt tcttgccaaa ttacatactc ttgccaagga gaaagtagtt
     5341 gttcatattt aggcaatagt cctgaaataa gtgactcttc ccatagcatt aacaaccaac
     5401 tattccaagg tatgatgaac ggtgatttcc agacactaaa ctgctgagat ataaaaattt
     5461 gttcactcag tccacgtaaa acacctgtta agcgttgatt acctgtcaga acgatagtgt
     5521 tctgatttga atcttttaat aactcattga tattgatttg ctttggattg agccaatcct
     5581 gtttcatagt cggtctgaat agagatcgta attaatcaat agtagaatgc ttactcgatt
     5641 cagagcaaat gagaaatttt catgttgcta gctgactgtt ggaaatgaaa taggtaaaat
     5701 tgcgcgttat gcttattttt tgtaatacaa atcgggagaa ggaatgaata acaattttat
     5761 caaaacagta ctttttattt tcatcgtttc ttggtctaat ttagcaattt cggagcaagc
     5821 tgagtcagct gcgcaggtgg cagagactca agaaaaggcg gatgattcag agatttttgg
     5881 ccagggctgg aaattagtcc gttctcctga aatgggaaat tctggtaaat ttgtccatat
     5941 ggtattagtc gatctggaca aagaaatgga taaaacggtt tatagtgctg cgattaaccg
     6001 cttatgtgga aaagcaaccg atttttgccg aattcgattc tggacacaag agcgctttat
     6061 tcctgaaaaa gtaacgccca ctcctgaaca gctcaaagct caaaaggcag actatctctt
     6121 taatcctact gcaggtgtaa agaaaacgac ttggtcgtgc aaagtggacc acgataaaag
     6181 tcattgcgta aattattgat cgtaacgaaa taccctcttt cttttattta attttatctg
     6241 agaaagaggg ggaaactccc tcgaccagat tattaaatca catctaacct agtccaacaa
     6301 attcaggatt aacaggttat cactcacatt tggcgactct tgataaagtg atgattgggt
     6361 aaagtgtttt gttcgtaaga attcagccac tcattaccaa gccgattgac aatcaattct
     6421 gacaaagtta atccctcatt ttttgcaagt tctgaaagta tttcccaagc attatgggtt
     6481 aggctcacag ttttaagttg aggcctatat ttctgctttt ctatttggtc ccttgcaata
     6541 ctgatggcat tcttaagttg tgcccactga gtttcatgca accacttttc acaccatgca
     6601 tttaaatcat taggattggc tttggcatcc tgatactcac gttcagcgac gataccttga
     6661 gacgcatctt cgcctagcca gtataaacct gatgagattt gtttatcgag gaattcaact
     6721 gcagcattct ggtcttgctg tctaattaca tatttttgtt tgttcatttt tattatctct
     6781 cattagtttc ataaaactac taagattttt tatgagtaga taatacaagc caattcatta
     6841 ttcaggcaag tctttattca gattt
//
LOCUS       DAOQKQ010000141         6862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_5500_length_6862_cov_6.332746, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000141 DAOQKQ010000000
VERSION     DAOQKQ010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 6862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6862
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5500_length_6862_cov_6.332746"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            303..878
                     /locus_tag="PKZ49_09440"
     CDS             303..878
                     /locus_tag="PKZ49_09440"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HND36825.1"
                     /translation="MATRQQFSDFLSDVEIRAYKQALYAVRDEQAALDIVQDSMLKLT
                     EKYSDKPDTELPLLFQRILQNTITDHYRRKKTRSTWITLFSAFSSESEEKDNEYSDLL
                     ETLQVNDDSINKYNPDVQLEQEQLIKLIEQAIETLPARQREAFLLRYWEEMDVYETAI
                     VMGCSEGSVKTHCSRATHALATILRAKGVKL"
     gene            875..1255
                     /locus_tag="PKZ49_09445"
     CDS             875..1255
                     /locus_tag="PKZ49_09445"
                     /inference="COORDINATES: protein motif:HMM:NF023699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HND36826.1"
                     /translation="MNEEEAVGKNIAKLLDQGLVNINQNTRYRLQAARRNALDRLHPS
                     EKIVHTGLGLSAHNGGHHWHFDLTKLFWLVLFLLAFIWATILGKSYFENDEIAVIDTK
                     ILSDDLPVDIYVDDNFDKWLNSTH"
     gene            1234..1614
                     /locus_tag="PKZ49_09450"
     CDS             1234..1614
                     /locus_tag="PKZ49_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HND36827.1"
                     /translation="MVKLNALITFWLFLIFPINAFSEQKHTWSSLKPYQKIILQPLEQ
                     EWDGMEPALKKKWIRITKHYLKRTPQEQQRIQSQMQNWSQLTTEQRKLARDRYKKLEN
                     LPPGKLLPIIEKWRKTEEIPKNSQ"
     gene            1679..3937
                     /locus_tag="PKZ49_09455"
     CDS             1679..3937
                     /locus_tag="PKZ49_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HND36828.1"
                     /translation="MSPLPPLWNEQVHPQLAKNETIVAWLELDLTDQLFFSKGILIVS
                     NRRLLSKMQNEEHWLSFVYQKELELTTHDHAGVGHIELFDTQSRLAHWCYTLGKLHFV
                     HHLVECFNQQSNFHLEGFPENLVTDSTYDEIGSTDKNSTSIEEQTSRETVSTFTLLRL
                     WRFAKPYRGRLLIGFLLMLASTAATLVPPYLTMPLMDEVLIPYQNGYPLNVDLATLYL
                     GGLLVAAILAWLLGWSKTYILAKVSERMGADLRITTYNHLLKLSHEYFGTKRTGDLMA
                     RIGAETDRINLFLSLHLLDFATDVIMMIMTAMILVSINPWLALVTLLPLPFIAWLIHL
                     VRDKLRTGFEKVDRVWADVNNVLADTIPGIRVVKAFAQEEREVKRFYDVNQRNLEIND
                     RVNKVWSLFTPTVTLLTEIGLLIVWIFGIWQVSENQITVGVLTAFLAYIGRFYLRLDS
                     MSRMVSITQKAAAGTKRIFEILDHVSRVPEPVKPIHSPTIQGHIELRHVGFRYGVRRI
                     TEDINLIIEPGEMIGLVGHSGSGKSTLINLICRFYDVTEGVILVDGIDIRSFPIAEYR
                     KHIGLVLQEPFLFFGTIAENIAYGKPHATRQEIVAAARAAHAHEFILKQLHGYDSLVG
                     ERGQALSGGERQRISIARALLINPRILILDEATSSVDSQTEQEIQAALDNLVRGRTTI
                     AIAHRLSTLRQANRLIVLDQGKIIEVGSHATLMAKRGYYYQLYLSQTGHIGVDPSLID
                     SNRESNQTKEWI"
     gene            3934..4407
                     /locus_tag="PKZ49_09460"
     CDS             3934..4407
                     /locus_tag="PKZ49_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HND36829.1"
                     /translation="MNYTKFKLSRDRHRKLILFDDAYQQKSHQVIAIRAFPISAADKG
                     IALVDHQGNELMWIENLQDLPVEWRELIEEELTSRDFMPEIKKIDSVSSFITPCVWWV
                     ETNRGNTCFTLKGEEAIRRLSSLALLITDQYGVHFLIKDRTKLDHYSIKLLDRFL"
     gene            4501..6789
                     /gene="cphA"
                     /locus_tag="PKZ49_09465"
     CDS             4501..6789
                     /gene="cphA"
                     /locus_tag="PKZ49_09465"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HND36830.1"
                     /translation="MHSNYTNRQFDLKFLEIIHLNGPNRWTYYPALEALVDIGALEDY
                     PSHIIPGFYDRLAAWLPSLIEHRCSYEERGGFLQRVREGTWPCHILEHVTLELQNLAG
                     MKGGFGRARETAERGVYWVVVSAWHAEITKQALFAARELILAAMGFEARQVDYDVQAA
                     IAQLRDMVDSLWLGPSTACIVDAATERNIPAIRLLSKGNLVQLGYGSNSRHIWTAETD
                     RTSAIAENISRDKELTKSLLRACGIPVPEGRITHSPEDAWQAAEALGLPVVIKPCDGN
                     HGRGVFIELSQCEEIKSAYSVALKEGSGVLVERFVSGFEHRLLVVNGELIAATRGDMI
                     SVVGDGISCIAELIEQQINSDSRRGTSEEHPLNLIRLDSAAQLEIAHQGYSADSVIPV
                     NARVLIQRNGNHAFDVTDEVHPSTAKIAALAARVIGLDIAGIDLVVEDISKPLLEQSG
                     AIVEVNAGPSLLMHIKPAVGKSRPVGKAIVEHLFPEKGNGRIPVIGITGSYGKTTVAH
                     LLTKLISLSGKRVGLASSKGLFFEHKRVDERNSANWSSANRVLMNRMIDVAVLENSFN
                     SLLRQGLAYDSCQVGIITNIDPNLHYGFYGIATPEQVYAVMRTQVDVVTPTAAAIDDV
                     IKEITLPMGSAILNARDSMLVTMSQLCHGEVIYFSVDPDLPSIKQHRIEGSGSASRKR
                     AVLVRDGSILLVDGTNEMVLIQLTELLPPTDSECIVVEIENVLAAIAAAWALDISPDV
                     MRVGIAAFGSSQQEAPCQSQFS"
BASE COUNT         2017 a         1379 c         1497 g         1969 t
ORIGIN      
        1 tgcagcggcc tgcaaagccg tctacgccgg ttcaattccg acccccgcct ccatgtactt
       61 ccctgccctt tgctcttata ggcgagtacc tatcagtagg ctccccttgt ttaatttctc
      121 cccattaact aaaacctcac actcgttttg ctatttataa tgatttgagg tgccttatga
      181 tcagatcatc cacaaaaata acaaatttaa tagtggattt cgattcattg tggcgtattc
      241 atcggcactt aaccaaatta aaccgattag ttccagtgag gctagattaa tagcatagta
      301 acttggctac aagacaacaa ttttctgatt ttttgagtga tgtggaaatt cgcgcttata
      361 agcaggcgtt atatgcagtt cgtgatgaac aagctgcact agatattgtt caggactcga
      421 tgcttaagct aactgagaaa tatagtgata aacccgatac tgaattgcct ctgctgtttc
      481 aacgtattct gcaaaatacc ataaccgatc attatcgtcg caaaaaaacc cgctcaactt
      541 ggattacgct gttttctgct ttttcttcgg aatctgaaga gaaagataac gaatattccg
      601 acctattaga aacattacaa gttaatgatg attctattaa taaatataac ccggatgttc
      661 aacttgagca ggagcaactc attaagctga ttgagcaagc gattgaaaca ttaccggctc
      721 gtcaacgaga agcattcttg ctgcgttatt gggaagagat ggatgtatat gaaacagcaa
      781 tcgtgatggg atgttctgaa ggaagtgtca aaacacattg ttcccgtgca acacatgcac
      841 ttgctacaat actaagagca aaaggagtca aattatgaat gaagaagagg ccgtagggaa
      901 aaatattgct aaattacttg atcaaggttt ggttaatatt aatcagaata cacggtatcg
      961 actgcaagca gcccgcagaa atgctttaga tcgattacat ccatcagaga agattgttca
     1021 caccggcctt ggtttatctg cgcataatgg ggggcatcac tggcatttcg acttaaccaa
     1081 gttattctgg ctagttctat ttttattagc ttttatctgg gcaactattc tgggaaaatc
     1141 ttactttgag aacgatgaaa tcgcagtaat cgatacaaag attctgtctg atgatttgcc
     1201 cgtagatatc tatgtagatg acaattttga taaatggtta aactcaacgc actaattaca
     1261 ttctggctgt ttttgatttt tccgattaac gcgttttctg agcagaaaca tacttggtca
     1321 tcactaaagc cttaccagaa aatcatttta caaccgcttg aacaagagtg ggatggaatg
     1381 gaacctgcgc tgaaaaagaa atggataaga ataaccaagc actatctcaa aagaacaccg
     1441 caagagcagc aacgaattca atcacaaatg caaaactgga gtcagttaac taccgagcaa
     1501 agaaaactag ctagagatcg ctacaaaaaa ttagaaaatt taccgccggg taaattacta
     1561 ccgattattg agaaatggag aaaaacagaa gaaataccaa aaaattctca ataaaacagc
     1621 ttcctcaatc acttcacaac gatggtgacc ccgtttccca tttaaaaaga gtagccttat
     1681 ttctcccctc ccaccgctct ggaatgagca agttcaccca cagttagcga aaaatgaaac
     1741 tatcgtcgct tggctggaat tggatttaac cgatcaatta tttttttcga aaggcatttt
     1801 gattgtttcc aatcgacgtt tactctcaaa aatgcaaaat gaggagcatt ggcttagctt
     1861 tgtttatcaa aaagagctag aactaacgac tcacgatcat gcaggcgtag gtcacatcga
     1921 actgtttgat acgcaatcac gactcgcgca ttggtgctat acgcttggca agcttcattt
     1981 tgttcatcat ttagtggaat gtttcaacca acaatcaaat tttcatctag aaggcttccc
     2041 ggaaaatttg gtcactgact ctacgtatga tgaaatagga tcaactgata aaaattcaac
     2101 atcgatagaa gagcaaacct ctcgagaaac tgtttctact ttcactttat taaggttatg
     2161 gcgatttgca aaaccatacc gagggcgttt actgataggt tttttgctaa tgcttgccag
     2221 tactgcagca acactggtgc caccttattt gacaatgcct ttaatggatg aagtgcttat
     2281 tccttatcaa aatggctatc cacttaatgt cgatctagca acgctttatc tcggagggtt
     2341 attggtcgca gccatcctcg catggctgtt aggatggtct aaaacttata ttctagcgaa
     2401 agtatcagaa cgaatggggg ccgatcttcg tatcacaacc tataatcatt tgctaaaact
     2461 ttcccacgaa tattttggaa ccaagcgtac aggcgattta atggcccgga ttggtgcaga
     2521 aactgatcgt atcaatttat ttctttcttt gcatttactc gattttgcta ccgacgtgat
     2581 catgatgatc atgactgcga tgatcttggt ttcaatcaac ccctggttag cattggttac
     2641 gctactaccc ctgcctttca ttgcgtggct aattcattta gtcagagaca aactccgaac
     2701 gggttttgag aaagtcgatc gtgtctgggc agatgtaaat aatgtgcttg cagataccat
     2761 accaggcatt cgagtggtaa aggcatttgc acaagaagaa cgggaagtaa aacgctttta
     2821 tgatgttaat cagcgcaatt tagaaattaa cgatcgagta aataaggtct ggtccttatt
     2881 tacgcctact gtaacattgc taaccgagat tgggctattg atcgtgtgga tttttgggat
     2941 ttggcaagta tccgagaatc aaattacggt aggcgtacta acagcatttt tagcttacat
     3001 tgggcgtttt tacttacgct tggattcaat gagtcgaatg gtttcaatta cccaaaaagc
     3061 tgcagcaggt actaagcgta tttttgaaat attagatcac gtgtctcgtg tacctgaacc
     3121 ggttaagcct attcactccc caactattca aggtcatatc gagttacggc atgtgggatt
     3181 ccgctatgga gtaagacgta tcactgaaga tattaatctg attatcgagc caggggaaat
     3241 gattggtttg gttggtcata gcggatcggg aaaaagtacg ctcatcaatt tgatttgtcg
     3301 tttttacgat gtcaccgaag gtgtcattct agtggatggc atcgatattc ggtcatttcc
     3361 catcgctgag tatcgaaaac atatcggatt agtcttacaa gaacccttct tgttttttgg
     3421 tacgattgca gaaaacatag cctatggaaa accacatgca acacgtcaag aaattgttgc
     3481 tgcagcacga gctgcccatg cgcatgaatt catactcaag caactccatg gctatgattc
     3541 ccttgttgga gaacgaggac aagcactttc tggaggagaa cgtcaacgga tttcaatcgc
     3601 acgcgcatta ctcatcaacc ctcgtatatt gatacttgat gaagcaacct catcggttga
     3661 tagccaaaca gagcaagaga tacaagccgc gttagataat ttagtacgag ggcgaactac
     3721 gatagcgata gcacaccgtt tatcgacatt acgccaagca aatcgactga tcgtgctcga
     3781 ccaaggaaaa ataattgagg taggcagcca tgccacacta atggctaaac gaggctatta
     3841 ttaccagctt tatttatctc aaacaggcca tatcggtgtt gatccgtcct tgattgattc
     3901 caatagggaa tcgaaccaaa cgaaagaatg gatatgaatt acacaaagtt taagctaagc
     3961 cgggataggc atcgcaagct gatactattt gatgatgcgt atcagcaaaa gagccatcag
     4021 gtgattgcga ttcgcgcttt cccgatcagt gcagcagata agggaatcgc cttggttgat
     4081 catcaaggca acgaattaat gtggatagaa aatctccaag atcttccagt ggaatggcgt
     4141 gagctgattg aagaagaact tacgagtcga gattttatgc ctgaaattaa aaaaatcgat
     4201 agcgtatcaa gttttattac accctgtgtt tggtgggtag aaactaaccg aggtaatact
     4261 tgtttcacat taaaaggtga ggaagcgatt cgtcgattat cctcattggc tttgctaatt
     4321 actgatcaat atggcgtaca ttttttaatt aaggaccgta caaaactcga tcactacagc
     4381 atcaaactcc ttgatcgctt tctctgagaa tcaaactaaa tatcagaatt ttatgtaaac
     4441 cgattgttgc ctactgcgtt agttaatggt gtttctccta ttcctattca aagtagcttc
     4501 atgcattcta actatactaa ccgtcagttt gatcttaaat ttcttgaaat tattcattta
     4561 aacggcccta accggtggac ctattatccg gccttagaag cattggttga tatcggtgca
     4621 cttgaggatt atccctctca tattattcct ggattttatg atcgcttagc agcttggttg
     4681 ccctctttaa ttgagcatcg ctgtagctat gaagaacggg gggggttttt acaacgagta
     4741 cgtgagggca cttggccctg ccatattctt gaacatgtca cattagaact acaaaacctc
     4801 gctggcatga aaggggggtt tggacgcgcg cgtgaaaccg ctgagcgcgg tgtctattgg
     4861 gttgtcgtta gtgcgtggca tgcagagatt accaaacaag cactatttgc agcacgtgag
     4921 ctgattcttg ccgcaatggg ctttgaagca agacaagttg attatgatgt tcaagctgcg
     4981 attgcccaat tacgggatat ggtagattca ttatggttgg gtccttcgac agcctgcatt
     5041 gttgatgcag cgactgagcg caatattcct gcaattcgtt tgctttccaa gggaaatctc
     5101 gttcaattag gctatggatc gaatagtcgc catatttgga ctgccgagac agatcgtaca
     5161 tccgccattg ctgaaaatat ttcgcgtgat aaagaactca ccaaaagttt gctgcgtgct
     5221 tgtggtatcc ctgttccaga gggacgtatt actcatagcc ctgaagatgc ttggcaagca
     5281 gcagaagcac tgggtttgcc agttgtcatt aaaccgtgtg atgggaacca tggacgaggg
     5341 gtatttatcg agttaagtca atgcgaagaa attaaatcgg cctattcggt tgctttaaag
     5401 gaaggcagtg gcgtattagt tgaacgattt gtatcaggat ttgagcatcg acttctcgtc
     5461 gtaaatggtg agctaatcgc agcgactcga ggagatatga tttccgtagt aggtgatgga
     5521 atttcctgca ttgctgaatt aattgaacaa caaatcaatt ccgactctag acggggaaca
     5581 agcgaagaac atccgcttaa tcttattcga ctcgatagtg ccgcccaatt ggaaattgcg
     5641 catcaaggtt attctgcgga ttcggttata cctgtcaatg caagagtgct cattcaacga
     5701 aatggcaatc atgcatttga tgtaaccgat gaggttcacc caagcactgc caaaatagct
     5761 gccctggcag cacgcgtaat cggcttggat attgccggca tcgatcttgt tgttgaggat
     5821 atctcaaagc ctttattaga acaatctgga gcgatcgtcg aagttaatgc cggccccagt
     5881 ctattgatgc atattaagcc agcagttggt aaatctcgtc cggttggtaa agctatcgtt
     5941 gagcaccttt ttcctgaaaa gggcaatggt cgtatcccag ttattggaat tactggaagc
     6001 tatggcaaaa ccactgttgc acacttgtta actaagctta tcagtttatc aggaaaacga
     6061 gttggtctgg cttcgagtaa ggggttgttc tttgaacata aacgagttga tgaaagaaat
     6121 tctgcaaatt ggtcgtcagc aaaccgcgtg ctaatgaatc ggatgattga tgttgcagtt
     6181 ctggaaaata gctttaatag ccttctaaga caaggattag cttacgatag ttgtcaggtg
     6241 ggaatcatta ccaatattga tcccaatttg cactacggtt tttacggaat tgcaacgcca
     6301 gaacaagtat atgctgtgat gcgaactcaa gtcgatgtgg taacaccaac tgcagcagca
     6361 atcgatgatg taatcaaaga gattactcta ccgatgggtt ctgctatctt gaatgcgaga
     6421 gactcaatgc tagtgaccat gtcgcaactt tgtcatggcg aagtgattta tttcagtgtt
     6481 gatccagatt tgccaagtat caaacagcac cgtattgagg gctcagggag cgcttccaga
     6541 aaacgagcag tattagtacg cgatggcagt attttactag tcgatggtac gaacgaaatg
     6601 gttttgattc aactcactga acttttaccg cctaccgaca gtgaatgtat tgtagtggaa
     6661 attgagaatg ttctcgcagc catcgctgct gcctgggcgt tagatatatc gcctgatgta
     6721 atgcgggtgg gaatcgcagc gtttggatca agtcagcagg aagcgccttg ccaaagccaa
     6781 ttttcatgaa ataacaaaag cacaagggta tagtatgaaa ttaatacaaa gtcgtgtatt
     6841 acgcggtcct aatttatgga gt
//
LOCUS       DAOQKQ010000142         6857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_5506_length_6857_cov_5.741988, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000142 DAOQKQ010000000
VERSION     DAOQKQ010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 6857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6857
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5506_length_6857_cov_5.741988"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="PKZ49_09470"
     CDS             complement(<1..327)
                     /locus_tag="PKZ49_09470"
                     /inference="COORDINATES: protein motif:HMM:NF022526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2780 domain-containing protein"
                     /protein_id="HND36831.1"
                     /translation="MNISIFRIFPLLSAMIILMSCSSPAQLPSSSGAANVLQQSTEAI
                     QTPTAAMSSVPTSDGLIGLLMQQLGVSQIQAEGGTGALLQLAQSKMSPGDFTALGNSI
                     PNMQSIM"
     gene            complement(435..1340)
                     /locus_tag="PKZ49_09475"
     CDS             complement(435..1340)
                     /locus_tag="PKZ49_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HND36832.1"
                     /translation="MNFLITGATGFVGQRLVARLKTEHCIKVLSRNRIHAERLLGVPA
                     YDWNYLLDPVPAEALEDTQVIIHLMGENLGNGRWTEQRKQEIYQSRILSTRKLVEATT
                     PNLKRFICGSAIGIYPGIGDSVYDETFVVPTNGNFMQTICRDWEDEVRKIENRGVRSV
                     SIRTGVVLGLGGMLEKLLPLFKLGLGGSIGDGKQWLPWIHIDDLVSVFIAAALDDHYQ
                     GPVNAVSPNPVQYQEFSETLGKVLHRPTFLTVPAFILKLALGEAAALALNSYRIEPAK
                     LIESYDFHFKFTQLGTALENIIQKS"
     gene            1465..3909
                     /locus_tag="PKZ49_09480"
     CDS             1465..3909
                     /locus_tag="PKZ49_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HND36833.1"
                     /translation="MNELTPQALRLTIDPNSLGFLDTSELIDQPLSWIGQERAEKAAY
                     FGLEMDHPDYNLFVLGEVGSGRSSLLKQAMTKVAQHKVVPADLCYIHNFEMPERPFVL
                     RVFAGKGRLLRQKLVQFVKYLQSEVPRYFEGQDFKAESERIERKFKLDEAQAYTKLDT
                     FAEQHHFAIQRESNRMIFTLLDNSGKVLTEAEVLALSKEKRQEFERLEQALQAEIASY
                     FDKVRPLERAKEEVLNDLQRSGVKRLLADELAMIRDGACISDEDQQKLNQFVTKMEED
                     ILDHLELFRISDAEEEKRLEELKSLLFCYQINLVTDNHELKGAPVVIDESPSYRSLFG
                     SIDYQSIEGAFTTDFTRIRAGSLLKAHGGFLMLYLDDLLVDNLVWEKLCRLLRSSKLQ
                     IEEPGVNLAQVATASLVPEAIDVQVKIILIGSREQYYLIQEENPELARRFRVKVDFAD
                     SFISNEQIRHAASIFVAHACQESNLPHFSAKAVARLLEDCHRDANDQLRQSAIFSRTE
                     TLVLESAAQCKVHAGCLVDVVDVETAIQARLFRHNYPDCCLQEAIADGDILIDTMGER
                     IGQINGLVQIDLGDHCFGAPVRITAHTYAGESGVLNIEREVGLSGPIHDKGVFILQSY
                     LSALFASIAPLALNASIVFEQEYSGIEGDSASCAELFALISALSGIPIKQGIAVTGAV
                     NQYGEILPIGGINEKIEGYFSVCKTAGLDGSQGVLIPQRNQHNLMLPRNVIEAVAQGL
                     FHVYTAQHIAEGVELMMGYPAGITAESNLHEIINYSHESVLGYAQRTLRAYRHACQQI
                     DRPKSEHKRFVRRTEK"
     gene            4072..4611
                     /locus_tag="PKZ49_09485"
     CDS             4072..4611
                     /locus_tag="PKZ49_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HND36834.1"
                     /translation="MRVIVLLLFVCVTQSAFAACQSGLLDHRFRKLAGTEEVNLCDTY
                     AGKVLLVVNTASKCGYTPQYDGLEKLHEQYEKRGFAVLGFPSNDFMGQEPGTEEQIQD
                     FCRLTYGVQFPMFEKVTVRSEQATAFFKQLAEVSGTYPTWNFHKFLIGRDGKFIKQFS
                     PHTTPADLDMINAIEKAIQ"
     gene            4657..6630
                     /locus_tag="PKZ49_09490"
     CDS             4657..6630
                     /locus_tag="PKZ49_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HND36835.1"
                     /translation="MRNHFPQIEVTPPLHRAGQSLSDSPSWLKSKRMLIFLIAFAVTA
                     VISMSYVLGRSSIYRSHATLLTMAKTAIDQRSVDVDLQHVAIQKQLLLGADMLAEIIK
                     TLQSHPEVKTQLTASDIRSMLDVQPVAETNLVEIVAEGPDPKILPILVNTWLDVYLKG
                     RENEIARATGDTTQRIQTEISGLTEKINQKRIELELFRENNHIVSTGREENEALSRLK
                     GLNDSLNKAIEEEVKAKARLDSIKKSIAKGQAVVPKEDTQKLAMLEKRAQELKEELAD
                     LDRRYTREYMALTPSLKVIPEKIATLQTEIDQLRSAGQRIVLSEAEQEYVAALQTTQA
                     IRDQLEAHKKKAADFTARFTEHEALKIDLEGFEQRLRETQERLIQIETKYGGKFPQVD
                     VVERAAVPLQPIWPDYSRDSLIALISSLIMGLIAVWVVEFLTRKEKAKLAINLSGVHI
                     YGGEESAREVVNQLPLTQQRMLAPQSVQPALIGGVIQEIAPQKIHLLFQAGNNKEKQL
                     MVLLLSGLTLDEITLLDPQCFDLENNQLSILGKYPRKIPLHPALRRLVEARGYNLIGA
                     SGHALNLDDLAVLLTCTIVDAGLTAAEGISADALRQTYLIYLVRQGMRLSDLAQIVGY
                     ITSTELTTLSQFAPLGPKLAASAVNLFYPLEAS"
BASE COUNT         1982 a         1407 c         1604 g         1864 t
ORIGIN      
        1 catgatactt tgcatattgg gtatcgaatt acctaacgct gtaaaatcgc ctggcgacat
       61 tttagattga gctaactgaa gtaatgcgcc tgtaccgcct tccgcttgga tttgagaaac
      121 gccaagttgt tgcataagga gaccaattaa cccatcactt gtaggaaccg agctcattgc
      181 tgcagtagga gtctgaatgg cttcagtgct ttgttgtaga acatttgctg ccccagaaga
      241 gctaggtaat tgcgcgggac ttgagcaact cataaggata atcattgctg atagtaaagg
      301 aaaaatacga aaaattgata tattcatgat agttttatta ctcctattta aggataaaat
      361 tgaaacgaat aaaaagaata acgatgttaa tcataagacg atgcaggtta ttggtaaatc
      421 aatttagctt aaggttaaga tttctggata atattttcta atgcagtacc aagttgcgtg
      481 aatttaaaat gaaaatcata ggactcaatc agtttggctg gttcgatgcg ataactgttc
      541 aatgctaaag cagccgcttc ccctaatgct aatttaagga taaaagcggg aactgtcagg
      601 aaagtaggcc ggtgtaaaac tttacccagg gtttctgaaa attcttggta ctgtacagga
      661 ttgggtgaaa ccgcattgac aggcccttgg tagtggtcat cgagcgcggc tgcgatgaaa
      721 acggaaacca aatcatcgat atgaatccaa ggaagccatt gttttccatc accaatggat
      781 cccccgagac cgagtttaaa caaagggagc aatttttcta gcataccacc gagacctaac
      841 accacgccgg tacgaatgct gacgctacga acccctctat tttcaatttt tctaacttca
      901 tcttcccagt cgcggcaaat ggtttgcatg aaattaccat tcgttggaac aacaaaggtt
      961 tcgtcataga ccgaatcacc gattcccgga taaatcccaa tagctgaacc acaaatgaaa
     1021 cgctttagat tcggcgtagt cgcttcaacc aatttgcgcg tacttaggat acgcgattga
     1081 tagatttctt gcttgcgttg ctcagtccat cgaccatttc ccagattttc acccatcaaa
     1141 tgaataataa cttgcgtatc ttcaagcgct tcggctggaa cagggtcaag caaatagttc
     1201 caatcatagg cagggacacc gagcaaacgt tcagcatgta tccgattcct gctaagtacc
     1261 ttaatgcagt gttctgtttt aagccgtgct acaagtcgtt gaccgacaaa ccccgtggcc
     1321 cctgtgatga gaaaattcat gttagttcac tgttctaatt gtgttcgatg cgtgatcaag
     1381 ataccctttt ctaagataga tagcaaagtg cagtaaaatt acttttttta acagccccgt
     1441 tcaagtaaac agattactta ttgtatgaat gaattaactc ctcaagcgct tcgcttaaca
     1501 atcgatccca actcattagg ttttttagat acatcggagt taattgatca accattgtca
     1561 tggattgggc aggaacgtgc agagaaagca gcatattttg gcttagaaat ggatcatccc
     1621 gactataatt tatttgtgtt gggagaagtg gggagcggtc gatcttcctt gctcaaacaa
     1681 gccatgacca aagtcgctca acataaagtc gttccagctg atttgtgtta tatccacaac
     1741 tttgaaatgc cagaaagacc ttttgtgctg cgcgtttttg caggaaaagg acgcttactg
     1801 cgtcagaagc tagtgcagtt tgtaaaatat ttacaatctg aagtaccgcg ttattttgag
     1861 gggcaagatt ttaaagcgga aagtgaacga atcgaaagaa aatttaagct ggatgaagca
     1921 caagcctata ctaagctaga tacgtttgca gagcagcacc attttgcgat tcagcgtgaa
     1981 tccaatcgaa tgattttcac attgctcgac aattctggaa aggttttgac agaagctgag
     2041 gtattggcat tatcgaagga aaagcgtcaa gaatttgaac gactcgagca agcgttgcaa
     2101 gcagagatcg caagttactt tgacaaagtt cgcccgcttg agcgtgctaa agaggaagta
     2161 ttgaacgatc tgcagcgcag tggggttaaa cggttattgg cggacgagct tgctatgatt
     2221 cgtgatggtg cctgtataag cgatgaagat cagcaaaagc ttaatcagtt tgtgacaaag
     2281 atggaggaag atatcctcga tcatcttgaa ctattcagaa tttcagatgc tgaagaagaa
     2341 aaacgcctgg aagaattaaa atcactgcta ttttgctacc agattaattt agtgactgat
     2401 aatcacgaat taaaaggagc ccccgtcgtt attgatgaaa gtccttctta tcgttcactg
     2461 tttggcagca tcgattatca atcgatcgaa ggggcattta caactgattt tactcgtatc
     2521 cgtgcaggga gtttgctcaa ggcgcatggc ggattcttaa tgctctatct ggacgactta
     2581 ctagtggata atctggtatg ggaaaagctc tgccgtctgt tacgcagtag taaattacag
     2641 atcgaagagc caggagtaaa tcttgcgcaa gttgcaacag cgtccctggt gcctgaagcg
     2701 atcgatgttc aggtcaaaat cattctaatc ggttcacgtg aacaatatta tttaatccag
     2761 gaagaaaacc cagagctagc acgacgcttc agggtcaagg tggattttgc ggatagtttt
     2821 atttcaaacg aacaaatacg ccatgctgct tcaatttttg tcgcacatgc ttgtcaagag
     2881 tccaatttac cccatttcag tgctaaagca gttgctcgct tattggaaga ttgtcaccga
     2941 gatgccaatg atcagttacg acaaagcgct atttttagtc gaactgaaac gttggtatta
     3001 gaaagcgccg cacaatgtaa ggtgcacgcg ggttgtttgg tcgatgtggt agatgttgaa
     3061 acggcgattc aagcgcgatt atttcgccat aattaccctg attgctgctt acaagaagcg
     3121 atcgctgatg gagatatctt aatcgatacg atgggtgaaa ggattggtca aatcaacggt
     3181 cttgttcaga ttgatttggg cgatcactgc tttggcgcgc ctgtccgaat taccgcgcat
     3241 acttatgcag gtgaaagcgg tgtactcaat atcgagcgag aagtagggct ttctggaccc
     3301 attcacgata aaggcgtatt tatcctgcaa agctatctgt cagccctgtt tgccagcatc
     3361 gcaccgcttg cgctcaatgc atcgattgtc ttcgagcaag agtatagcgg tattgagggt
     3421 gattctgcat cctgtgcaga gctattcgcg ctgatttccg cgctttccgg aatacccatc
     3481 aagcaaggaa ttgccgtgac cggggccgta aatcagtacg gagagatttt acccatcggt
     3541 ggcatcaacg aaaaaatcga gggttatttt agtgtatgta agactgctgg attagatgga
     3601 tcgcagggtg tgctcattcc gcagcgtaac cagcataact tgatgctccc gcgcaatgtc
     3661 atcgaagcag tcgcacaagg gttgtttcat gtttataccg cacagcacat tgctgaaggg
     3721 gttgaattaa tgatgggtta tccagcagga attactgctg aaagcaattt gcacgagatc
     3781 attaattatt ctcacgagag tgttctgggt tatgcacaga gaacattacg cgcttatagg
     3841 catgcgtgtc agcaaatcga tcgacctaaa tccgagcata aacgctttgt gcggcgcact
     3901 gaaaaatagc caacagggtt gactgtatga attttctgat ggtgattatc aaatagcgct
     3961 agcttagcaa ttgtggaggc agtagttgtc taacgctagc aggtttattg tgaatacttg
     4021 tatgaatgta cggttcgtca tatccttcaa ttaacaacaa ggagaattcc gatgcgagtt
     4081 attgtattgc ttttgtttgt ttgcgttact caatctgctt tcgcagcttg tcaaagtgga
     4141 ttgttggatc atcgtttccg gaagctggca ggaactgagg aggtcaatct ttgtgatact
     4201 tacgcgggaa aagtgctatt ggtcgtcaat accgcgagta aatgtgggta taccccgcaa
     4261 tacgatggat tagaaaaatt acatgagcaa tatgaaaaac gtgggtttgc tgtattaggt
     4321 tttccttcga atgattttat gggacaggag cccggcactg aggaacaaat ccaagatttt
     4381 tgccgcctga cttatggcgt gcagtttcct atgtttgaga aagtaacggt cagaagcgaa
     4441 caggcaactg cttttttcaa acaattggcg gaagtttcag ggacttatcc aacctggaat
     4501 tttcacaagt ttctgattgg tcgtgatggg aaatttatta agcagtttag tccccatacg
     4561 acgcctgctg atcttgacat gattaatgcc attgaaaaag ccatccagtg attgggaaat
     4621 gatttttttc ctttatgttg ataattcggt gatcgagtga gaaatcattt tcctcaaata
     4681 gaagttaccc cgcctttaca tcgagcaggg cagtcgttat ctgattcacc atcatggcta
     4741 aaatcgaagc gaatgctgat ttttctcatc gctttcgctg taactgcagt tatttcgatg
     4801 agctatgtgt tgggacgttc atcaatctat cgtagtcatg caaccttgct gaccatggca
     4861 aaaacagcaa tcgaccaaag gagtgtcgac gtcgatcttc agcatgtcgc aatccaaaaa
     4921 cagttgctac tgggtgcgga tatgttggca gagatcataa aaacgctcca atcgcatccg
     4981 gaggttaaaa cccagttaac tgcttcggat atccgtagca tgcttgatgt acagccagtc
     5041 gcagaaacga atttggtcga gatcgtcgcg gaagggcctg atcccaaaat cttaccgatt
     5101 ctagtcaata cctggctcga tgtttacctt aaaggcagag aaaacgagat tgcccgggca
     5161 acgggtgata cgacgcaaag aattcaaacc gagatcagcg gtttaacgga gaaaatcaac
     5221 cagaagcgca tcgagcttga gttatttcgt gaaaacaatc acatcgtttc tactggcagg
     5281 gaagagaatg aagctttatc aagactaaaa gggctaaatg actcgttaaa taaagcaatt
     5341 gaagaagaag tcaaagccaa ggcgcgcttg gattcaatta aaaaatccat tgccaaaggt
     5401 caagccgtgg tgcctaaaga agacacgcaa aaattggcga tgctggaaaa gcgtgcacag
     5461 gaattaaagg aagagcttgc tgatttagat cgtcgctata ctcgcgaata tatggcgcta
     5521 acaccttcgt taaaggtcat tccagagaaa atagcgacgt tacaaactga aatcgatcaa
     5581 cttcgtagcg cgggccaaag gattgtgctt tcagaagctg aacaagaata tgttgctgcg
     5641 cttcagacca cacaagcgat tcgggatcaa ctagaagcgc ataagaaaaa agcggctgat
     5701 tttacggcac gctttacgga gcatgaagca ttaaaaattg atctcgaagg gtttgagcaa
     5761 cgattacgcg aaacacaaga acgattgatc caaatagaaa ccaagtatgg cgggaaattc
     5821 ccacaagtcg atgtagtgga acgtgctgcg gtacctttac agccaatttg gcccgattat
     5881 tcacgagatt cactgattgc tctcataagc tcgctcatca tgggtttgat tgcggtttgg
     5941 gtggttgagt ttttaacccg caaagagaag gccaagttag cgattaatct ctcaggggtg
     6001 catatctatg gcggtgaaga atctgcgcga gaagtagtca atcagttacc gctgacacag
     6061 caacgcatgc tagcgccgca atcagttcag ccagccttga tcggtggcgt cattcaggaa
     6121 attgcccctc agaaaatcca cttgctcttt caggcgggta acaataaaga aaaacaactc
     6181 atggtattgc tactgagtgg gttaaccctg gatgaaatta ccctactaga tccgcagtgt
     6241 tttgatcttg aaaataacca gctttccatc ctaggcaaat atccgagaaa aataccgctt
     6301 catcccgcat tgcggcgatt agtcgaagca cgtggctata acctaattgg agcatcaggc
     6361 catgcactta atctggatga tctggcggtt ttgctgactt gcacaattgt tgatgccggc
     6421 ctgaccgcag cggagggcat ttcagcagat gcattacggc aaacctatct gatttatttg
     6481 gttaggcaag gaatgcggct ttcagatctg gcgcaaatcg tgggatacat cacctcgact
     6541 gaattaacga cattgagtca atttgcaccg ttagggccta agttagcagc gtcagccgtt
     6601 aatttatttt atccgctgga agcttcctag cagatcgtcg aatcgatttt atacctgaca
     6661 cgatcaggtt tcattgcaag cagttaattt cttagccgga gggatatgct acgaaaaatt
     6721 tttaccacgc ttggttttgg ggcttccgcg atgatgccga gcgagccgcc ctacactccc
     6781 tatcagagtg acgctgccaa tgagatttat aatttactgt tctgcgataa tctagcagcc
     6841 ttcaaaccca aggaagg
//
LOCUS       DAOQKQ010000143         6844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_5519_length_6844_cov_5.681838, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000143 DAOQKQ010000000
VERSION     DAOQKQ010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 6844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6844
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5519_length_6844_cov_5.681838"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..1362)
                     /locus_tag="PKZ49_09495"
     CDS             complement(145..1362)
                     /locus_tag="PKZ49_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HND36836.1"
                     /translation="MTNKDFRPEKKISIFYPPQRVLMGPGPSDTHSRVLSAMARPTLG
                     HLDPVFTDMMEELKSLMRYAFQTNNLLTFPVSGPGSVGMEMCFVNMIEPGDKVIVCRN
                     GVFGGRMIENVTRHGGEAIIVDDKWGEPVDPQKVEDALKKNPGTKIVAFVHAETSTGV
                     QSDAKTISEIAKRYNCLTIMDTVTSLGGTPIYIDGWGIDAVYSGSQKCLSCPPGLSPV
                     SFSDRVVELVKNRKHKVQSWFMDINLLLGYWGSSRTYHHTAPTNALYGLHEALLMLYE
                     EGLEHSWARHQRNHKALKAGLQTMGIEYVVAEPYRLPQLNAVHVPVGIDEKEVRRRLL
                     ADFNLEIGAGLGDFAGKIWRFGLMGTSSRVENVAFCLNALEVVLADLGMKVERGSAEV
                     AAHQTYAQNPMLG"
     gene            complement(1468..2967)
                     /locus_tag="PKZ49_09500"
     CDS             complement(1468..2967)
                     /locus_tag="PKZ49_09500"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HND36837.1"
                     /translation="MSESTLSNFINLLVYSLATVMGLVVVIAIVALFIRDITQKQHTL
                     LRNYPIIGRLRYFFEIQGKYFRQYLFSGDRDEMPFNRATRTWVYKSAKNKGGIVGFGS
                     TYDLREPGAFIFVNAAFPILEEDQLPTPPVIIGENYCQYPFQAKSVINISGMSYGAIS
                     APAVRALSLGAAQAGCWLNTGEGGLSDYHREGNCDIIMQIGTAKYGVRDAEGNFSPTR
                     TKAIAEFVKAFEIKLSQGAKPGKGGVLLGAKVNPEIAAIRGIPQYEDSISPNRHYDIR
                     NINELLDKVAYIRELTGKPVGVKTAIGGWHFMNELCETILRRGLDYAPDFLTIDGGEG
                     GSGAAPQTLMDHAGLPIAEALPRVVDSLIESDLKKRIRVIAAGKLVTSAHGAWALCVG
                     ADFINSARGFMFSLGCIQAMRCHLNTCPTGITTHNPRLQHGLVVEEKYLRVANYAMNV
                     NKEINMIAHSCGLKHAREFRREHVRIVETAGKSIALNRLYPYPEIRKKA"
     gene            complement(3405..3971)
                     /locus_tag="PKZ49_09505"
     CDS             complement(3405..3971)
                     /locus_tag="PKZ49_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36838.1"
                     /translation="MIKQSIQLTISTCILVLCYVGTVFGHGKVPLEDDVCVRMAGGNM
                     VHLSTYQPQFDVNEQYCTEIPTEGETYLVVDLLDPALRNMPVSVKVFRGGSEDGETVA
                     QINADYYSDGVINGIGRLDKGLYSVVVTAEGVPPLNYHYQLRVAMLDYGKVARSMIGP
                     MIAMLILSWIAYRFLQSRRIRFPFNLRQ"
     gene            complement(4383..4940)
                     /gene="efp"
                     /locus_tag="PKZ49_09510"
     CDS             complement(4383..4940)
                     /gene="efp"
                     /locus_tag="PKZ49_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HND36839.1"
                     /translation="MKTAQELRVGNVFMLGKDPMVVLKSEFTKSGRNSSVVKMKFKNL
                     LTESPSEAVYKADDKFDIVVLDKKEVTYSYFAEPMYVFMDAEYNQYEVEAETMVDALN
                     FLEDGMPCEVVFYNGKAISVELPNTVVREIVYTEPAVKGDTSGKVMKNAKITTGFELQ
                     VPLFCEIGEKIEIDTRTLEYRSRVK"
     gene            complement(5108..5521)
                     /locus_tag="PKZ49_09515"
     CDS             complement(5108..5521)
                     /locus_tag="PKZ49_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36840.1"
                     /translation="MPLNDGEEPKLKPLGPGGGEVQEKEKALIAEIIAKVNDLFEGDL
                     TDDDQLIYVNNVLKGKLLESEILIQQAMNNTKEQFSNSPDFSKEFMNAIMDALAAHST
                     MSRQALDSERVRSGLKDILLGPAKLYEALRERQQS"
     gene            complement(5529..>6844)
                     /locus_tag="PKZ49_09520"
     CDS             complement(5529..>6844)
                     /locus_tag="PKZ49_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HND36841.1"
                     /translation="TLELFGRRPNPDQQVSADNLPAPFHVYSMRQAIEEGFILDVLQN
                     YTPYKLAFKLAHNGKEMSEKEVERSEAMKHIMRWVKLHPYNISQKVQVVVEHFRENVA
                     PLLDDQAKAMVVVGSRLEAVRWKLTIEKYIKDQNYKIGTLVAFSGEVNDSESGPEPFT
                     ENSTILNPNLRGRDMREAFKAEDYQILLVANKFQTGFDQPLLCGMYVDKRLAGIQAVQ
                     TLSRLNRAYPGKDTTYVVDFANDGEEILAAFKTYHTTAELENITDPNLVFDLRMKLDA
                     AGYYDDFEVDRVVAVEFKENAKQGDLFAALEPVASRLLHRYKTAQEHLKIAKDRNNSK
                     DTEEAQNELNALILFKRDMGTFQRMYAFLAQIFDYGNTAIEKRFIFYKRLLPLLEFGR
                     EHKGIDLSKIILTHHNFKSRGKQAMPSHSRKIQDCSRESQLQESR"
BASE COUNT         1892 a         1540 c         1416 g         1996 t
ORIGIN      
        1 aagtcaaaag aaatagttca atcggaaaaa atagaatggt ttaaacctaa attggaatca
       61 gaatttgtta attaacatta acgaatatcg agaagccatt tcctatcaac aggaaatggc
      121 catgattatt tattagggag atatctatcc tagcattgga ttctgagcat aagtttgatg
      181 tgcggctact tctgcgctgc ctcgttctac cttcataccc aaatctgcaa gaacgacttc
      241 taatgcattt agacagaaag caacattctc aactcggctg gaagtaccca tcaaaccaaa
      301 gcgccaaatt tttcctgcaa agtcccccag tcctgcaccg atttctaaat tgaaatcagc
      361 caataaacgg cgacgtactt ctttttcatc gataccaacc ggaacatgca ctgcattcaa
      421 ttggggtagg cggtagggtt cagcaacgac gtactcgata cccattgttt gcaatcctgc
      481 ttttaatgct ttgtgattgc gttgatgtct tgcccatgaa tgttctagac cttcttcata
      541 taacatcagt aatgcttcat gtaagccata taacgcattg gtcggtgcgg tgtggtgata
      601 agttcgcgat gaaccccagt agcctaggag taaattaata tccatgaacc agctttgaac
      661 tttgtgttta cgattcttaa ctaattctac aacacgatca gagaagctga ctggcgatag
      721 cccagggggg caggacaagc atttctgact gccagagtaa accgcatcga ttccccatcc
      781 atcaatatag atgggagttc caccgagaga tgttactgtg tccattatcg ttaggcagtt
      841 atatcgttta gcgatttcac tgatggtttt tgcatctgac tgaacacccg tcgatgtttc
      901 tgcgtgaacg aaagcaacga ttttagtgcc agggtttttc ttcaacgcgt cctcgacttt
      961 ttgagggtca accggttctc cccatttgtc atcgacaata atcgcttcgc cgccatgacg
     1021 tgtaacattt tcaatcatgc ggccaccaaa aaccccattt ctgcagacga tgaccttatc
     1081 gcctggctca atcatattta cgaaacacat ttccatacca actgacccag gcccagagac
     1141 cgggaaagtt agtaaattat tagtctggaa ggcatatcgc atcaggcttt tgagctcttc
     1201 catcatatca gtaaacacag ggtctaaatg accaagcgtt ggacgtgcca tggcagacag
     1261 tacacgggag tgagtatcag atggccccgg tcccattaaa acgcgctgtg ggggataaaa
     1321 gatcgaaatt ttcttctctg gacgaaagtc tttatttgtc attgttattc ctaatgggtt
     1381 taaaaagcag tgattattaa aatattgaac cactaatttt atcagaagtg atcaataatt
     1441 tgaggactga atcgtgccaa gctcagatta ggcttttttg cgaatttcag ggtaaggata
     1501 aagtctattc aaagcaatgc tcttgcccgc agtttctacg atacgaacat gctcacggcg
     1561 aaactcacga gcatgcttta atccgcacga atgggcgatc atgttgattt ccttatttac
     1621 attcattgca tagttagcta cgcgtagata tttttcttca accactaacc catgttgtaa
     1681 gcgcggatta tgggtggtta tgcctgttgg gcaagtgttg agatggcagc gcattgcttg
     1741 aatgcatccc agtgaaaaca taaagccacg ggctgagttg ataaaatctg caccgacaca
     1801 aagcgcccag gcaccatggg cggaagtaac gagctttcca gctgcgataa cccgaatacg
     1861 tttttttaga tccgattcaa tcagtgagtc gacaacgcgc ggtaacgctt cagcgattgg
     1921 taatcctgcg tgatccatca atgtttgagg tgcggccccg cttccccctt caccaccatc
     1981 gatagttaaa aaatcggggg cgtaatctaa tccacgtcgt agaatagttt cacaaagctc
     2041 attcataaag tgccatcccc caatggcagt ttttacgcct accggttttc ccgttagttc
     2101 tcgaatgtag gcaactttat cgagcaattc gttgatgttc ctaatatcat agtgtcggtt
     2161 ggggctaatg gagtcctcat attggggaat accacggata gcagcgatct caggatttac
     2221 ttttgcgccg agtaatacac caccttttcc tggtttggct ccttgagaaa gtttaatctc
     2281 aaaagcttta acaaattcgg cgattgcttt agtccgtgtc ggcgaaaaat tgccttccgc
     2341 atcgcggaca ccatattttg cggtaccgat ttgcataata atgtcacaat taccttctcg
     2401 gtgataatcc gagagccccc cttctccagt gtttaaccag caacctgctt gggctgctcc
     2461 caaggataaa gcgcgaactg ccggggctga aatcgcacca taactcatcc cactaatgtt
     2521 gataaccgat tttgcctgaa aagggtactg gcagtagttt tctcctataa ttacgggtgg
     2581 cgttggtaat tgatcttctt ccaaaattgg aaatgcagca ttgacgaaga taaaggcgcc
     2641 aggctctcgc aagtcataag tagaaccaaa tccaacgata ccccctttat tttttgcact
     2701 cttataaacc catgttctag tagcacgatt aaagggcatt tcatctctat cacctgaaaa
     2761 caagtattgc cgaaagtatt tcccttgtat ctcaaagaaa tatcggagtc gaccaatgat
     2821 tggatagttg cgtaacagcg tatgctgttt ttgcgtaata tcgcgaataa acagcgctac
     2881 gattgcgata acgacaacta accccatgac tgtcgctaag ctataaacca gtaagttgat
     2941 gaaatttgat aaggtagatt cagacatgtc ttcagaaata agatgtggta atgacagcta
     3001 tttttttaga gaaaaagata gccatagatt aacgcgcaac aagcatttga taaatacgtg
     3061 gattaaattt atcaataatg atgatagaga attgcttagg aatgatgggt tgatactcaa
     3121 acaacgggcg aggcgctttt cctcaatagt ttgtaccaaa tgaaagagag ggtattggaa
     3181 ataattaaac caatacaagc ggtggggggc gatgatgtat ctatgaatgg attggttagc
     3241 ccattagcta tcccgattgc cgagctaaat agctcattga ttttgtttct ttagtaattt
     3301 tatctggaaa ctaggggaca ctaaacgttg ttaaaaagga aactttgtct aaggattatt
     3361 ccaccctctg ctaaattgtc agagggtgat agagcgtaaa tggtttattg gcgtaaatta
     3421 aagggaaatc tgatccgcct tgactgtagg aagcgatagg caatccaaga tagaatcaac
     3481 attgcaatca ttgggccaat catacttcgg gctactttgc cataatcaag cattgctaca
     3541 cgaagttggt aatgataatt caaaggcggc acaccctcgg ctgtaacaac gaccgaataa
     3601 agccctttat ccaacctgcc aataccattg atgacgccat ctgagtaata gtcagcattt
     3661 atttgtgcga cagtctctcc atcttctgag cctccacgaa atactttcac acttactggc
     3721 atatttctta aagcagggtc aagtaaatcg acgacaaggt aggtttcgcc ttctgtggga
     3781 atttcagtgc aatattgttc attcacatca aactgaggtt gataagtact gagatgaacc
     3841 atgtttcctc cagccatgcg tacacaaaca tcatcttcta gtggaacctt accatgacca
     3901 aaaacggtac ctacataaca tagaacaagg atgcaggtcg atatagttaa ttgtattgat
     3961 tgttttatca cgacaaccct cctgtgttta tcaaaaattt tctgttgttg ccaatttaag
     4021 tgtttttttc atttgtattc catttcatgg atctacaatg agatcggaca gcatgagtta
     4081 aattttgttc atttaggtaa gaatcaaaac ttaaaattac tttaacgggc ggttgtaaaa
     4141 ttacctacga tcccgctgca aaacagactt aaatacgcta tttattagat ataacctgcg
     4201 cttttttatc tgtttctgcc ctaagattga cttcctcaga aatgtttttc aacagcctgt
     4261 tagaacccaa agataggtgt agcaatgcaa aaataaaaaa acttttgttg gattttaaga
     4321 tttggtttta gcgaaaaaaa aggagccatt gggctccttt tttaagaaca gatagcgaga
     4381 acttacttaa ctcgactccg atactccagt gttctggtat caatttcaat tttttcacca
     4441 atttcacaaa acagagggac ttgcaattca aagcctgtag taattttagc gtttttcata
     4501 accttaccgg aggtatcacc ctttacagcg ggctcggtat aaacgatttc acgcacgact
     4561 gtgttaggaa gttcaaccga aatagccttg ccattataaa atacgacttc acaaggcatt
     4621 ccatcttcta aaaagttaag tgcatctacc atggtttctg cttcaacttc atactgatta
     4681 tattcagcat ccatgaaaac gtacattggt tcggcaaagt aagaatacgt aacttctttc
     4741 ttatctaaaa caacgatatc gaacttatca tctgctttgt aaaccgcctc gctgggtgac
     4801 tcagtcagaa gattcttgaa tttcatttta acgactgagg aatttcgacc tgatttggtg
     4861 aattctgatt tcaacacaac catggggtct tttccaagca taaatacatt tccaacacga
     4921 agttcctgag ctgttttcat ctagatgaat gcaatgagta gaattaaaaa cagaattata
     4981 cgctgagtga gtcaaaaaaa tccagttgtg ttatagctat aaaaagatag cttataactt
     5041 agattaagct ttatatttca atgaagtagc ttaacctttg caaagcatgg atttgtcgtt
     5101 atttctttta tgactgttgc cgttcccgca acgcttcata aagctttgca ggccccagca
     5161 atatatcctt caaaccgctg cgtacccgct ccgaatccag cgcctgcctg ctcatcgtgc
     5221 tatgtgccgc aagagcatcc ataattgcat tcataaattc tttggagaaa tcaggagagt
     5281 tcgagaattg ttccttggtg ttattcattg cctgttgtat caggatttcc gattccagca
     5341 atttaccctt tagcacatta ttgacataaa tcagctggtc atcatcggtc agatcgccct
     5401 cgaacaggtc attgaccttg gcgatgattt cagcgatcag cgctttttcc ttttcctgca
     5461 cctcgccgcc accggggcca aggggtttaa gctttggttc ctccccatca ttgagcggca
     5521 ttgcttgttt accgcgactc ttgaagttgt gattcgcgtg agcaatcctg tattttcctg
     5581 ctatgactgg gcattgcttg tttaccgcga ctcttgaagt tgtgatgggt cagaatgatc
     5641 ttggatagat cgataccttt atgttcacgg ccaaattcca gcaatggcag caagcgttta
     5701 tagaaaatga aacgtttctc aatcgccgtg ttgccgtaat caaaaatctg cgccaaaaac
     5761 gcatacatgc gctggaatgt tcccatatcc cgtttgaaca ggatgagtgc attcaattcg
     5821 ttctgcgctt cttctgtatc cttgctattg ttccggtctt tggcaatctt gagatgttcc
     5881 tgagcagttt tgtatcgatg cagcaggcgt gaagccacgg gttccaatgc cgcaaacagg
     5941 tcgccttgct tggcattttc tttgaattcc accgccacca cgcgatctac ttcgaaatca
     6001 tcatagtatc cagccgcatc cagcttcatg cgcagatcaa acaccaggtt cgggtcggta
     6061 atgttttcca gttccgctgt ggtgtgatac gttttgaatg cagccaggat ttcttcgcca
     6121 tcattcgcaa aatccaccac ataagtcgtg tccttaccgg gataggcacg gttcagccgc
     6181 gataaggttt gaactgcctg aataccggcc agccgcttat ccacgtacat gccgcacaat
     6241 aagggctgat cgaaaccggt ctggaatttg ttggcaacca ataggatttg atagtcttcg
     6301 gctttgaacg cttcgcgcat atcgcggcca cgcaggttgg gattaaggat tgtgctgttt
     6361 tccgtgaacg gctccgggcc ggattcgcta tcattcacct cgcccgagaa tgcaaccagc
     6421 gtgccgatct tgtaattctg atccttgata tatttctcaa tggtcagttt ccagcgcaca
     6481 gcttccaggc ggctgcctac caccaccatc gctttggcct gatcatccag caatggcgca
     6541 acattctcgc gaaaatgctc cactacgacc tgcactttct gcgaaatatt gtacggatgc
     6601 agcttcaccc agcgcatgat gtgcttcatt gcttcgctgc gctcaacttc cttctcgctc
     6661 atctctttgc cgttgtgagc cagcttgaat gcaagtttgt acggcgtgta attctgcaat
     6721 acgtccagaa taaaaccttc ctcgatggcc tgccgcatgg aataaacatg gaagggtgca
     6781 ggtagattat ccgcgctgac ttgctgatca ggatttggcc gccgcccgaa caattcaagt
     6841 gtct
//
LOCUS       DAOQKQ010000144         6835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_5537_length_6835_cov_5.980973, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000144 DAOQKQ010000000
VERSION     DAOQKQ010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 6835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6835
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5537_length_6835_cov_5.980973"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PKZ49_09525"
     CDS             <1..262
                     /locus_tag="PKZ49_09525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HND36842.1"
                     /translation="LLEALGCGNAVIAHNNVFNREVADNAALYFSTATEIPNLVEKID
                     TDETLRAAMRLNARNIVSQRYTWKLVTDAYMALLTAPSSTEW"
     gene            283..1245
                     /locus_tag="PKZ49_09530"
     CDS             283..1245
                     /locus_tag="PKZ49_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HND36843.1"
                     /translation="MMTRHIDIFNGDADGICALIQLRLAKPVESQLITGVKRDIALLQ
                     KVEASSGDQITVLDISLDRNRSDLDRLISVGAHITYFDHHYAGQIPQSSYLNTVIDES
                     PDTCTSLLVDRYLNGQYSLWAITAAFGDNLVQVAEQRCIMAHLNSIDTQKLRQLGELI
                     NYNGYGTQIDDLHFHPAKLYQALKQFQDPREAYDEAPEITTLVTGYTEDMEHAKMMIA
                     EIATSTAAVYRLPDTAWARRAVGVWANDLSQDFPARAHLIICPDGENSYTVSVRAAKD
                     HPYGASAFCRRYPGGGGREAAAGINKLPESNLAELIQAFALAFK"
     gene            complement(1249..1662)
                     /locus_tag="PKZ49_09535"
     CDS             complement(1249..1662)
                     /locus_tag="PKZ49_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HND36844.1"
                     /translation="MVVSTKSFLTLEDAKLVAAAAEAEALRNNWSVVIAIVDEGGHLI
                     YLQRLDNTQIGSVDVAIQKARSAIAFRRPTKVLEEQINQGHLRYLGLPGAIPIEGGLP
                     IVFKDQFLGAIGVSGVRSYQDAQIAQAGIDALLPQ"
     gene            complement(1683..2633)
                     /locus_tag="PKZ49_09540"
     CDS             complement(1683..2633)
                     /locus_tag="PKZ49_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HND36845.1"
                     /translation="MTSVNKVSDIQLKSTYLEIDEIVDALRDLRIASLGNRQGHLRPP
                     KLPSRKILISVAEGLNAAMFPNRLGSADLADEGVDHYVGHTLNMTLRALLVQIQHELH
                     SNSGLEALGDIDREKSVAITQAFAKRLPAVRNLLESDIVAAYEGDPAARSVDEVLACY
                     PGIMAIAYYRLAHELHLLGVPLIARMISEIAHSQTGIEIHPGAQIGPRFFIDHGTGVV
                     IGETAIIGQNVRLYQAVTLGAKRFPVDEKGALVKGIARHPIVEDDVVIYAGATILGRI
                     TIGKGSTIGGNVWLTRSVPPGSNVTQAQMRYELFEGGSGI"
     gene            complement(2633..3610)
                     /gene="cysK"
                     /locus_tag="PKZ49_09545"
     CDS             complement(2633..3610)
                     /gene="cysK"
                     /locus_tag="PKZ49_09545"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HND36846.1"
                     /translation="MSNWFKDNSRSIGRTPLVQLNRVTDGASATVLAKIEGRNPAYSV
                     KCRIGVAMVDDAERRGLLGPGKEIVEPTSGNTGIALAFVAAARGIPLTLTMPETMSIE
                     RRKLLVAFGAKLVLTEGSKGMNGAIAKANEIVESEAGRYVLLQQFKNAANPAIHELTT
                     GPEIWNDTNGAIDIFVAGIGTGGTMTGVSRYIKQAKGKPILSVAVEPSASPVISQRRA
                     GEPLTPGPHKIQGIGAGFIPDNLDLSLVDAVEQVSNEEAVVYARRLAREEGILSGISC
                     GAAVAAAVRQAKRAENAGKTIVVILPDSGERYLSSVLFEGMFNEQGLSA"
     gene            3913..>6835
                     /locus_tag="PKZ49_09550"
     CDS             3913..>6835
                     /locus_tag="PKZ49_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HND36847.1"
                     /translation="MMQQSTFFSVSLVLGIVSLAFAYYVGAILGLSIPYIGSNITLFW
                     PPAGIALAVLTLWGLALCPGILLGSLVVTLTIGELALPAALGVSLGNTLGPLAGAYIL
                     RKIAGFPTHFSNKQNIIRFLIISAGTSALTASFGTLILYLNDKILIEQLQLAWLGWWS
                     GDMIGILVFAPPFFFWSTQSIKNFLLNLTTRWEFILAISSCIGLSWIVFIGFTPLNAI
                     TSAIPYIVYLPLLWIGLRFNSMAVAIAVIVISSLATIGTSQGLGPFSEGNFYTDQFLL
                     WVFITTTSLISFMMISIQSSRKQVEQNLRDSESRLRLALEAANQGLYDLNIQTDQAIV
                     SPEYARMLGYDPNLFQETTEKWLERMHPEDREHTYQIYKDYINGVKSNYEVEFRQLTR
                     AGEWKWILSLGKVVEWDDQGHPLRMLGTHTDISDFKAKELVLQQNEEALHRTQIIAKL
                     GSWHFDLIQDVLTLSKEAFRIFGAGRQAPFSFSSFLAHVLPEDRENVNLAWQAFLKGD
                     PYDIEFRFKLNARTKWVRVRAEITTLDSNSVPIKAVGTIQDISERKQAIEEITQAKNL
                     LRTVIDASPDMISVKDKQHRFLLVNQSLATSQGLEPAAMLGKPDTDFWPDYQCYGDSA
                     RNIRGFHVDDNEAFSGKTVHNPADQALLANGELRWFDTIKIPLRNMQGHITAILGCAR
                     DITDRLHIESKYRALIEQIPAVTYTIAIDPEIHTVYVSPQLETQLGFSVEEWLANPQL
                     WLNQIHPEDRKQILSSLSVSLTTGSPYYSEYRIYKKDGSLTWVRDDAVWLKDAMGKPV
                     LLQGVMLDITHQRQAEEKLNTALEELRNSERFQRELRTLAEREQSRLGALLSAMNIGI
                     LFEDTDRRIEYINPTFTRMWNITEQVYLLNKPIQTLLERSNEIFSKPAHASKYLLNLL
                     NSDEITKLSELELNNGRILTQSSYPVNDAEHKLIGRLWIYEDITQERQTAQQLLYLAE
                     RD"
BASE COUNT         1963 a         1602 c         1360 g         1910 t
ORIGIN      
        1 gttactcgaa gcattgggtt gcggtaatgc cgttatcgca cacaataatg tttttaatcg
       61 cgaagtcgct gacaatgcag cactttattt ttctacagca acagagatac ctaatctagt
      121 agaaaaaatt gatactgacg aaacacttcg tgcagcaatg cgcttaaatg ctcgaaacat
      181 tgtatcccag agatatacct ggaaactagt caccgacgct tatatggcgt tacttacagc
      241 accatcctcg actgaatggt gataattgct taaagaaaaa tcatgatgac tcgccatatt
      301 gatatcttca atggagacgc agatggtatt tgcgcgttaa tacaattgcg cttagccaag
      361 cctgtcgaat cacagctcat aacgggcgtt aaacgcgata ttgctttact ccagaaagtt
      421 gaagctagct caggagatca aatcacagtg cttgatatct cactggatcg taaccgcagt
      481 gatttggatc gattaatttc tgtgggggca catattacct attttgatca tcactatgcg
      541 gggcagatac ctcaaagcag ctatttaaat acagtgattg atgaatcccc ggatacttgc
      601 accagtctac tcgttgatcg ttatctcaat gggcagtatt cattatgggc gattaccgct
      661 gcgttcggcg ataacctagt ccaggttgct gagcaacgct gcattatggc gcatcttaat
      721 tcaattgata cacaaaaact gcgccaattg ggtgagttga tcaactacaa tggatatgga
      781 acgcagatcg atgatttgca ttttcatcct gccaagcttt accaagcact aaagcaattc
      841 caagatccca gagaagctta cgatgaagca ccggaaatca ctactttggt tactggttat
      901 acagaagata tggaacacgc caaaatgatg attgccgaaa ttgccacatc cacagcagcc
      961 gtttatcgat tacctgatac agcttgggcc cgtcgtgcgg taggggtttg ggccaatgac
     1021 ctttcacagg attttcctgc tcgcgcccat ttgatcatct gtcctgatgg cgaaaattca
     1081 tacacagtta gtgtccgtgc agcaaaagat catccttatg gcgcatcggc attctgtaga
     1141 cgctatcctg gtgggggtgg acgagaagcc gcagccggaa ttaataaact ccctgaatcc
     1201 aatctcgcag agttaattca agcgttcgcg ttagctttca agtaagtttt actgaggtaa
     1261 taaagcatcg ataccagctt gtgcaatctg cgcatcttgg tatgaccgaa caccactaac
     1321 accaatagca cccaagaatt gatctttaaa cacaataggt aatccgccct caatcggtat
     1381 tgctcccggt agccctaggt atcgcaagtg cccttgattg atctgttctt cgagtacttt
     1441 agttggccgt ctaaaagcaa tcgccgaacg tgctttttgg atggcaacat caacactccc
     1501 aatttgagta ttatcaagtc gctgcaagta tattaaatgc ccaccttcat cgactattgc
     1561 gataacaact gaccaattat tgcgtaatgc ctccgcttca gctgcagctg caacaagctt
     1621 tgcatcttcc agcgtcaaaa aacttttggt actaaccaca atttctcctc agaaaaatga
     1681 tattaaattc cacttccccc ttcaaataac tcatagcgca tttgtgcttg agtaacatta
     1741 ctccccggtg gaacactacg ggtcaaccaa acattcccac cgatagtaga acccttacca
     1801 atcgtgatac gccccaagat tgtcgcacct gcatagatta cgacatcatc ttcaacaatc
     1861 gggtggcgcg caatgccttt gactaaagca cctttctcat caaccggaaa tcgcttagcc
     1921 cctaaagtga ccgcttgata aagacgcaca ttttggccaa tgatcgccgt ttcaccgatt
     1981 accactccgg ttccatggtc gataaagaat cttgggccta tttgagcacc tggatgaatt
     2041 tcaatcccag tttgcgaatg cgcaatttca gaaatcatac gcgcaatgag tggaacccca
     2101 agcagatgta attcatgggc caatcgataa taggcaatgg ccataatccc agggtagcaa
     2161 gccagcactt catcaacact tcgtgcggcc ggatcacctt cataagccgc cacaatatca
     2221 ctttccaata aattcctcac ggcaggcaat cgtttagcaa aggcctgtgt aattgcgact
     2281 gatttttcac gatcgatatc accgagcgcc tcaagtccag agttagagtg aagctcatgc
     2341 tgaatttgta ccagcagtgc gcgcaaagtc atattgagcg tatgcccgac ataatgatca
     2401 accccctcat ccgctaaatc tgctgaaccc aaacgattgg ggaacatggc cgcattgagt
     2461 ccctcagcga cactaataag aatcttgcgg gatggtaatt taggaggacg tagatgacct
     2521 tgtcgattcc ccagtgaggc aatccttaaa tccctcagcg catcgacaat ctcatcgatt
     2581 tccaagtaag tgcttttaag ctgaatatcg ctaaccttat taacactagt cactatgcgc
     2641 ttagtccttg ttcattaaac atgccttcaa aaagaacgct actcaaatac cgttctcccg
     2701 agtcaggcag gatcactacg attgttttgc ccgcattttc agctcgtttc gcttgcctaa
     2761 ccgctgcagc tacagctgcg ccacaagaga ttccagaaag aatcccctct tcccgagcta
     2821 atcgacgcgc atatacaact gcttcttcat ttgacacctg ctccaccgca tccaccagag
     2881 aaagatcaag attatcggga ataaaccctg ccccgattcc ttgaatttta tgtggcccag
     2941 gtgttaaggg ttcacctgca cgtcgctgag aaatcactgg gcttgctgaa ggctcaactg
     3001 ctactgatag aataggctta cctttagcct gtttgatata tcgcgataca ccggtcattg
     3061 ttccccctgt tccaataccc gcaacaaaaa tatcaattgc accattggta tcgttccaaa
     3121 tctcaggccc cgttgttagc tcgtgtattg cgggatttgc agcattctta aattgttgta
     3181 gcagaacata tcgacctgct tctgattcca cgatttcatt tgcttttgca attgcaccat
     3241 tcatcccctt tgaaccctca gtgagtacca gttttgctcc aaaggcaact aataatttac
     3301 gacgctcaat actcatcgtt tctggcatcg ttaacgtaag cggaatccct cttgcagcag
     3361 caacaaatgc taaagcgatg cctgtatttc cgctggttgg ttcgacgatt tcttttccag
     3421 gacccaacaa ccccctccgt tctgcatcat caaccattgc caccccgatt cgacacttta
     3481 cagaataggc tgggttacgc ccctcaattt ttgcgagtac cgttgccgat gcaccatcag
     3541 tgactcgatt cagttgaaca agtggtgttc ggccaattga ccttgagtta tccttaaacc
     3601 aatttgacat gtgctacccc tttcatataa gtcttctgac aattttatat tgaaactcag
     3661 aatgaattag atttgatact ctattttact cgttcctaaa tcttagctaa attattgatc
     3721 tacctgatag gctagtttta aagtttttat taaataaaaa ttagaatagt gatatgaaac
     3781 ccatctattt taaagttaaa catagcacta attgaatgct atgctcatcc aactatcgat
     3841 gaaaatgcag ctagtcaaaa ccctatttag ccaactcagt agattttctt taccgaatat
     3901 gcttaaccat tcatgatgca acaatcaacc tttttctcgg tttcattggt tctgggaata
     3961 gtttccttag cattcgctta ttacgttgga gcaatactgg gcttgagcat accgtacatt
     4021 ggctcaaata tcaccctatt ttggccacct gctggaattg ctttggcagt attaacacta
     4081 tggggattag cgctttgccc cggtatcctt ctcggctcat tggttgtcac gctaacgatt
     4141 ggtgaattag ctttgcctgc cgctttaggt gtttcattag gtaatacact gggtccattg
     4201 gccggtgctt atattcttcg aaaaatagca ggttttccta ctcacttttc taataaacaa
     4261 aatattattc gctttcttat tatcagcgct ggaacttcag cgctcacagc tagttttggc
     4321 acgctgatac tctatctcaa tgacaagata ctcattgagc aacttcagtt agcatggtta
     4381 gggtggtggt ctggggatat gattggtatc ctagtttttg ctcccccatt ctttttttgg
     4441 tcaactcaat ccatcaaaaa cttcctactc aacttaacaa ctagatggga attcatttta
     4501 gcaattagta gttgtatagg attatcttgg atcgttttca tcggatttac cccacttaat
     4561 gcgatcacat cggctatccc ttatattgta taccttcctt tattgtggat tgggcttcgc
     4621 tttaattcaa tggcagtggc aatagcagtt atcgttatct catcattagc caccataggc
     4681 acctcgcaag ggcttggacc cttctcagaa ggaaatttct ataccgatca attcctgtta
     4741 tgggtattta tcacaaccac ttcgctcatc tcattcatga tgataagcat tcaatcatca
     4801 cgaaaacagg ttgaacaaaa tttgcgggat agtgaatcgc gccttagatt agcacttgaa
     4861 gcagctaatc aagggttgta tgacttaaat attcaaaccg atcaagcgat cgtcagtcca
     4921 gaatatgcac gtatgcttgg ttatgatccc aatttatttc aggaaaccac cgaaaaatgg
     4981 cttgaacgga tgcacccaga ggatcgcgag catacctatc aaatttataa agattatatt
     5041 aatggcgtaa aaagcaatta tgaagtggaa ttccgccaac ttacccgagc tggggaatgg
     5101 aaatggattc tatcgctagg caaggtagta gaatgggacg atcaagggca tccactgcgg
     5161 atgctaggca cgcataccga tattagtgac tttaaagcaa aagaactggt gcttcaacaa
     5221 aatgaagagg cgctgcatcg cacacaaatc atcgcaaaat taggaagctg gcatttcgat
     5281 ctgatacagg atgtccttac cttgtctaag gaagcctttc ggatattcgg tgccggaagg
     5341 caggcacctt tcagctttag tagtttttta gcgcatgtac tcccagagga tcgagaaaac
     5401 gttaatctgg cttggcaagc gttcctaaaa ggagaccctt acgatattga gttccgtttt
     5461 aaactcaatg cacggacaaa atgggtcaga gtcagagccg aaattacgac actggatagt
     5521 aatagtgttc ctatcaaagc cgttggaacc attcaagata tcagcgaacg taaacaagca
     5581 attgaagaaa tcactcaagc caagaatttg ctccgcacgg tcattgatgc ttctcccgat
     5641 atgatttctg taaaagataa gcagcatcgt tttctacttg tgaatcagtc attagctacc
     5701 agccaggggc tagagccagc agcaatgtta ggcaagcccg atacagattt ttggcctgac
     5761 tatcaatgtt atggtgattc agcccgaaac attcggggat ttcatgtaga tgataacgaa
     5821 gctttctcag gcaaaactgt gcataatcca gcagaccaag cactattagc caatggcgaa
     5881 ctccgctggt tcgatacgat taaaatcccg ctgcgtaata tgcaaggaca tattaccgcg
     5941 atattaggtt gtgctcgtga tattacggat cgactccata tcgaatcaaa ataccgcgcc
     6001 ttgattgagc agattccagc agtaacctac acaatcgcta ttgatccaga aatacatact
     6061 gtctatgtca gtccacagct tgaaacccaa ctagggttta gtgtcgagga atggttagcg
     6121 aatccacaac tatggttaaa tcaaattcac cctgaagatc gcaagcaaat attatcaagc
     6181 ttatcggtca gtttaacaac gggatcgcct tattattcag aatatcgtat ctacaaaaaa
     6241 gatggcagtc tcacttgggt aagagatgat gcagtttggt tgaaagatgc gatgggtaag
     6301 cctgtgctac tccaaggtgt catgcttgac atcacacacc agcggcaagc cgaggaaaaa
     6361 ttaaatacgg ctttggaaga attgcgtaat tccgaaagat ttcagcgcga attacgaacg
     6421 ttagccgagc gtgaacagag tcgattaggt gctttacttt ctgcgatgaa tatcggtatt
     6481 ttatttgaag acaccgatcg acgaatcgaa tacatcaacc ctactttcac gagaatgtgg
     6541 aacatcactg aacaggtcta tttattgaat aaacccattc aaacactttt agaacgatct
     6601 aatgagatat tctcaaaacc ggcgcatgcc tcaaaatatt tattaaacct acttaattca
     6661 gatgaaatta ctaagttatc agaattagaa ctgaataatg gtagaatcct gactcagtca
     6721 tcctatccag tcaatgatgc agaacataaa ctcatcggac gcctctggat ttatgaagat
     6781 atcacgcaag aaagacaaac tgcccaacaa cttctgtatc tcgcggaacg agacc
//
LOCUS       DAOQKQ010000145         6801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_5575_length_6801_cov_6.105989, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000145 DAOQKQ010000000
VERSION     DAOQKQ010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 6801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6801
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5575_length_6801_cov_6.105989"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(528..2477)
                     /locus_tag="PKZ49_09555"
     CDS             complement(528..2477)
                     /locus_tag="PKZ49_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HND36848.1"
                     /translation="MLIQKASIIPATNQLSPAFFSATFTQYYSLKSLIPAYLICIWVL
                     LITFNTNAAPPAFINQNLTIQSSEAELLLEKPHPIKGTEKEEWITGRILVMPRAGLPN
                     KAFASILKEHQGKAQKIGQSDLYIVNLPEYTEEGVIAKLKHHPHIKYAELDYLLHSSF
                     VPNDQYYSKAWHHTKIGTPTAWDSTRGAGITIAILDTGVDNTHPDLTSNIVPGWNFYS
                     NNSNTADVNGHGTAVAGVAAASTNNTIGVASVAGSAKIMPVRVADANATASASTVAKA
                     LTWVADKGVRVANISYSGIPGNLTIQNAAQYMKNKNGLVIVAAGNSGTDLGYTATTTM
                     IPVSATDSNDVKTSWSSFGKYVAISAPGKDIWTTFRNTVYGIGWGTSMATPVTAGTVA
                     LMMAANPKLSSTQVESLLFSTAKDLGTAGRDSYYGYGRVNAAAAVQAAKAASTTTTLL
                     TAATDSEKPVASIINPANGATVSGLVSVDIEASDNVGVIRTELWVNNTNVAIDTLPPY
                     AFTWDSTGVPNSTANLVVRAYDEAGNSTTSDAISVTVSNQTTIAVNDTTPPQVSIVNP
                     ISGSVAGNVTITMNASDNSGSAGITLSLYIDNNLKAVGSGSTLSYNWNTKTKSVLEGG
                     HVIKAIAKDASGNTSTSTVNVTVVK"
     gene            2854..>3074
                     /locus_tag="PKZ49_09560"
     CDS             2854..>3074
                     /locus_tag="PKZ49_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaA family lipoprotein"
                     /protein_id="HND36849.1"
                     /translation="MISYRYFLLPSVFILAACSSVENRQDPLETMNRAVFEFNEKFDY
                     YVFEPMAKGYKFITPDPLEMVIGNVFSNAG"
     assembly_gap    3075..3174
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3175..3423
                     /locus_tag="PKZ49_09565"
     CDS             <3175..3423
                     /locus_tag="PKZ49_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaA family lipoprotein"
                     /protein_id="HND36850.1"
                     /translation="DSSLFHPFWSIYPDFDFLTVRLPTAVGWSIDTRAQMLDIDKTLE
                     EASLDKYEFVRDAYLQRRRSLVHDGNPPKEEQNGENGE"
     gene            complement(3535..4422)
                     /gene="htpX"
                     /locus_tag="PKZ49_09570"
     CDS             complement(3535..4422)
                     /gene="htpX"
                     /locus_tag="PKZ49_09570"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HND36851.1"
                     /translation="MKRILLFIATNLAILIVLSITLRILGVDRVLDAQGGGLNFSALL
                     VFSAVLGFGGSLMSLAMSKWSAKHMTGAQVIEIPSNATEGWLVETVRKQAHAAGIGMP
                     EVAIYDSPDVNAFATGMSRNNALVAVSTGLLANMQQDEAEAVLAHEVSHIANGDMVTL
                     TLIQGVVNTFVIFLSRIIGHAVDRLIFKTEEEHGPAYMITSIIAQIVLGILASIIVMW
                     FSREREFRADAGGAKLAGRQKMIAALERLKQQYEPSHLPDKIATFGISGKQSQISRLF
                     MSHPPLDERIAALRGDTIA"
     gene            4642..5796
                     /locus_tag="PKZ49_09575"
     CDS             4642..5796
                     /locus_tag="PKZ49_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HND36852.1"
                     /translation="MSQQPVVLIGSGLAGYGVARELRKLNSEIPITMLSADHGGFYSK
                     PMLSNAIATGKTPESILNSSAVQMANQLHITIRPDTRVSVIDSAQKAIVLENGEHLIY
                     GQLVLAMGADQIRLPLQGDGVADLLTVNDLDDYRHFRVALEGKKQIAILGAGLIGCEF
                     ANDLVGAGYEVHVIDISTQPLGRLLPSEGGRFLQEKLTAIGIKFHLKTITQRIDRMGD
                     RLSLSFDDGTQLEVDLILSAVGLRPRIQLAQAAGIEVNRGIVTDRYLQTRTPDIYALG
                     DCAEVEGKVLPFIMPISHASRALAVTLNGTPTLLRYPAMPVLVKTPACPTIVSPPDPD
                     KEGEWRVDKTEDGIKALFQDITGNLLGFALLGDATKEKNALTPLLPAVLD"
     gene            5955..6494
                     /locus_tag="PKZ49_09580"
     CDS             5955..6494
                     /locus_tag="PKZ49_09580"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HND36853.1"
                     /translation="MSSRNSGWHPVPVSASSALRWWLLNQHSLTDLIKQRCNQFRVEP
                     TFQALARACHDELSVIDLHRNELALIREVYLYCGDRPVVFAHSVIARNHLQGVWRGLS
                     RLGNQSLGSLLFSNPLIKRQDRFSFRQLNDHHLLYHRACQRMSPNKPRRLWARRRSFS
                     LYGQPILVTEIFLPGILEL"
     gene            6491..>6801
                     /locus_tag="PKZ49_09585"
     CDS             6491..>6801
                     /locus_tag="PKZ49_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HND36854.1"
                     /translation="MSLITKERLIAYARLIRLDKPIGILLLLWPTLWGIWLAAEGTPD
                     AMILFIFIAGTILMRSAGCAINDFADRRIDPHVERTKNRPLAVGVITAKEAVVVASGL
                     S"
BASE COUNT         1823 a         1281 c         1544 g         2053 t
ORIGIN      
        1 atgcctgttt gagtcacacc cagattaaac tgaaaatagt ttagaggcat attaatctat
       61 gatttggttt tttttaactt tctgaatttt aaaacgtgat aaggattaaa ttttccttaa
      121 ttatttatta gcaaaaaaca tgccacaaag aaatataaat ataacattat gattttaaat
      181 taatatctta attaagatca tgactttgct gagtgatttt ttgaggtcat aaaagatgta
      241 aaaataatcg acaatttaaa tgagctcgaa atgatttttg cgataagaaa taaaaataga
      301 aaatagagta cggattattt cattttttta actggctgtc tttgaagtga tttttaaaga
      361 aaaaagatat gacacttttt tgcagagcgt tggaagaaat tgttaacaat aagttactct
      421 atcttgattg taaaattatt cgacaacctc aataacaagt atattcggct taggccaaga
      481 taaacgcctt aacgctatct tggcctagaa aaaggataaa gcatttttta tttaacaact
      541 gtaacattga ctgtgcttgt cgaagtattg ccagaagcgt ctttggcgat tgctttgatg
      601 acatgaccgc cttctaatac actctttgtt ttagtattcc agttatagct caatgtgctc
      661 ccggatccga cggcttttaa attattatcg atataaagcg ataaggttat tcctgctgag
      721 ccactattat cagacgcatt cattgtgatc gtaacgttac ctgccaccga tcctgatata
      781 gggttgacga tgctaacttg tggcggcgtt gtatcattta ctgcaattgt cgtttggttt
      841 gagacagtga cactaattgc atctgaggtt gtgctgttac cggcttcatc ataagcgcga
      901 acgacaagat tagcagtgct gttaggaaca cccgttgagt cccacgtaaa tgcataaggc
      961 ggtagcgtgt caatcgcaac attggtattg ttcacccata attcagtccg gatcacgcca
     1021 acattatcgc ttgcttcgat atcgactgat actaatcctg aaacggttgc accattggca
     1081 ggatttatga tgctggctac tggtttttcg ctgtccgttg ctgcggtaag aagagtcgtt
     1141 gtggttgacg ctgcttttgc tgcttgtact gctgccgcag cattcactcg cccataaccg
     1201 taataactat ccctccctgc cgtacccaga tctttggctg ttgaaaaaag taaactctca
     1261 acttgagtac tacttagttt gggattcgct gccatcatca aagcaaccgt gcccgcggtg
     1321 accggtgtgg ccattgaagt accccaaccg attccataaa cggtattccg gaatgtagtc
     1381 caaatatctt ttccgggagc tgatatggct acatatttcc caaagctaga ccagcttgtt
     1441 ttaacatcat tactatcagt tgctgaaact ggaatcatcg tcgttgtggc agtatagcca
     1501 aggtctgtgc cactatttcc agcggctacg atgactaatc catttttgtt tttcatgtat
     1561 tgcgctgcat tttgaatggt aaggttacca ggaatgcctg agtagctgat atttgcaaca
     1621 cgcacacctt tgtctgcgac ccaggttagc gcttttgcta cagtgctagc gcttgcggtg
     1681 gcattggcat cggcaacacg cactggcata attttcgctg aacctgcaac tgatgctaca
     1741 ccaatagtgt tgttagtgga ggcggctgct acgcccgcaa ctgcggtgcc atggccgtta
     1801 acatcagcag tatttgagtt gttgctatag aaattccagc caggcactat gttcgatgta
     1861 agatctgggt gggtattatc aacgcctgta tcgagaattg cgatggtaat gccagcgcca
     1921 cgggtgctat cccaagcggt tggggtccca attttagtgt gatgccaggc tttggaataa
     1981 tattgatcgt taggaacaaa actggagtgg agcaggtagt ctagttctgc atatttgata
     2041 tgcggatgat gttttagctt ggcaataacg ccttcttctg tgtattccgg cagattgacg
     2101 atatataggt cactttgacc aattttctgg gccttgcctt gatgttcttt caggatactt
     2161 gcaaaagctt tgttaggtaa tcctgcgcga ggcatgacaa gaatgcgccc ggttatccat
     2221 tcttcttttt cagtaccctt gattgggtgg ggtttttcaa gtaataactc tgcttcagaa
     2281 gattgaattg tgagattctg attgatgaat gccggtggcg ctgcgtttgt attaaaagtt
     2341 attaacaata cccatataca aatgagataa gcaggaatga gcgacttaag gctgtagtat
     2401 tgcgtaaatg tcgcagagaa aaaggctgga gataattggt ttgttgcggg gataatgctg
     2461 gctttttgaa taagcattag gtgcttctcc tttttttcta tttgttaatt gaaaatcata
     2521 ctcatgcctg tcaagcaatg actttagggg gtaggataat tgctaattgt ataaatcctt
     2581 tattagtcag caataagcat gccaaatatt aattcattaa taaacagtgg gttagaattg
     2641 aatctgctgg agttctgaag tcaagtgtcg ctgctctgag acgaaggggt gaatgacttg
     2701 atgtaactca ctgtattaaa tgatggtttt tacgtcgtca atcaatgaca gcgaataaaa
     2761 taggggaaat tcaaagggta aagataaagt aggttgtctc aactgcgtaa ttatggcaag
     2821 atgccctatc catgtaataa aaagaatttt aatatgattt catatcgcta ttttttgttg
     2881 ccatcggtgt ttattttggc tgcttgctcc tcggttgaga accggcagga cccgcttgaa
     2941 acgatgaatc gtgctgtttt cgagtttaat gaaaaatttg actattatgt ttttgagcct
     3001 atggcgaagg ggtataaatt tattacacct gatccattag aaatggtcat tggtaatgtt
     3061 ttctcgaatg cgggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngatagt
     3181 tcgcttttcc atccattctg gtcaatttat cccgattttg atttcttaac ggttcggttg
     3241 cctactgcag tgggttggtc gattgatacg cgtgcgcaaa tgctcgatat tgataaaacg
     3301 ttggaagaag cttcgctgga taaatatgaa tttgtgcgag acgcttactt gcagcggcgt
     3361 agaagccttg tgcatgatgg taatccgccc aaggaagaac aaaatggtga gaacggagaa
     3421 taaatttcgc accaaagaag ttctggcata agttgttttg ttgttaataa aaaggcctga
     3481 atgatttatc attcaggcct ttattttttt aagttgagtt ctctactgta attattatgc
     3541 aatcgtatcg ccacgaagtg ctgcaatcct ttcatcaagc ggtgggtgcg acataaataa
     3601 ccgacttatc tgactttgtt ttcccgaaat accaaaagta gcgatcttat ctggtaggtg
     3661 tgaaggttcg tattgttgtt taagccgttc cagtgcagcg atcatttttt gtcggcccgc
     3721 taatttagcg ccacctgcat ctgcgcgaaa ttcacgttcc cgactgaacc acatgacgat
     3781 gatgctcgcc aaaataccca atacgatttg agcaataata ctcgtgatca tgtaagcggg
     3841 gccgtgctct tcttctgttt tgaagatgag gcgatcaacg gcatggccaa tgattcggga
     3901 taagaaaatt acaaaagtat ttaccacgcc ctgaatcaaa gtaagcgtga ccatatcacc
     3961 attcgcaata tggctcactt catgcgctag cactgcttca gcttcatctt gctgcatgtt
     4021 ggctaatagt ccggtactca ccgccactag tgcgttgttc cgactcattc ccgtagcaaa
     4081 agcgttaaca tcgggcgaat catagattgc tacctctggc atgccgatac cagcagcatg
     4141 cgcttgtttg cgtacggttt caactaacca gccctctgtt gcattggatg gaatctcgat
     4201 gacctgagca ccagtcatgt gtttggctga ccatttggac atggcgagtg acatcagaga
     4261 tccaccaaag cccaataccg cagagaaaac cagtaaagca ctaaaattta gaccgccacc
     4321 ttgcgcatca agtactcgat ctacgccaag tattctgagc gttatactca gtacgataag
     4381 aatagcaaga ttagttgcta taaataacag tattcgtttc ataacatatt acctattcaa
     4441 tagtgagata aaaattattg gaatcatcta catttttttg gttcaatttg actctattgt
     4501 agataggggt gagtagttat tttatcaagc atagtggtta aagagaaaca gttcatccct
     4561 gaaaataaaa ctaagcgggt tgaggtacaa tacagatcaa atgataaaga caacactgat
     4621 ttttaaaacg atggagatcg aatgagtcaa caacctgttg tgctgatagg aagtgggtta
     4681 gccggttatg gcgtagctcg ggagctgcgc aaactcaatt cagaaatacc aattacgatg
     4741 ttatcagcgg atcatggcgg tttttattcc aagcccatgt tatcaaatgc gatagcaacg
     4801 ggtaaaaccc ctgaatcgat tttgaattct agtgcagtgc aaatggccaa tcagcttcat
     4861 attacaattc gacctgatac gagggttagt gtgattgatt cagctcaaaa agcgattgtg
     4921 cttgagaatg gcgaacactt gatctatggc cagctagtgc tagcgatggg agcagatcag
     4981 attcgtttgc cgctgcaagg agatggcgtt gcggatctat taaccgttaa tgatttggat
     5041 gactaccgcc attttcgggt tgcattagaa ggaaaaaaac aaattgctat tctaggtgcg
     5101 ggcttgatcg gatgtgaatt tgccaatgat ctggtcggcg cagggtatga agtgcatgtg
     5161 attgatatta gcacgcaacc actgggtcgt ttgttgccct ccgaaggggg gcgtttttta
     5221 caggaaaaat tgacagcaat tgggatcaaa tttcatttga aaaccataac tcagcggatt
     5281 gaccgaatgg gagatcgatt aagcttgtcg tttgatgatg ggactcagct tgaagtcgat
     5341 ttgattttat ctgcggtagg attgcgccct cgtatccagt tagctcaagc agcgggcatt
     5401 gaggtcaatc gcggtatcgt aacggatcgt taccttcaaa ctcgtacccc agatatctat
     5461 gcattaggag attgtgctga agtagaagga aaagtgttac cgtttattat gccgatcagc
     5521 catgcatcac gtgctttagc ggtaacactg aatggtacgc cgactttgtt gcgttatcct
     5581 gcgatgccag tattggttaa aacgccagct tgtccgacca ttgtttctcc acctgaccct
     5641 gataaagaag gggaatggcg tgttgataaa acagaagatg gaattaaagc attatttcag
     5701 gatatcacag gcaatttatt gggttttgca ttgctgggtg atgcgaccaa agaaaaaaat
     5761 gctttgactc ctttactccc tgcggtgctt gattgattct cagcgcagaa gttgatcaat
     5821 taatactgat tattgaatta gaacgagact aaagcattct gagattgggt gatagatgtt
     5881 attgcaatta aaaccaggtt ggggattgtg ttggctgagt agtgttaatc gattaagtcg
     5941 agtactgttt agcaatgtct agccgcaatt caggatggca tcctgttcct gtctcagctt
     6001 catctgcgct tcgatggtgg ttactcaatc aacattcatt aactgattta atcaagcagc
     6061 gttgtaatca atttcgtgtt gagcccactt tccaagcgtt ggctagggca tgccatgacg
     6121 agctaagtgt aattgacttg caccgcaatg aactcgcgtt gattcgtgag gtttatttat
     6181 attgcgggga taggccagta gtgtttgccc attcggtgat tgcgcggaac catttacagg
     6241 gagtatggcg cggattaagt cgcttaggta atcaatcgct gggttcgttg ctgttcagta
     6301 atcctttgat taagcggcaa gatcgattta gctttagaca gcttaacgat catcatttgc
     6361 tttatcatcg cgcttgtcaa agaatgtcac caaacaaacc aagacgattg tgggcgcggc
     6421 gtagatcgtt ctcactatat ggtcaaccta ttttagttac tgaaattttt ttacctggta
     6481 ttttagagct atgagtttga ttaccaaaga acgtcttatt gcttatgcga gactaattcg
     6541 actggataag ccgattggta tcttgttgtt actatggcca actttatggg ggatttggtt
     6601 agcggctgaa ggtacgccgg atgcgatgat tctgtttatc tttatagcgg gcacgatact
     6661 gatgcgttcg gctggctgcg ccattaatga tttcgcagat cgacggatcg atccgcatgt
     6721 tgagcggacc aaaaaccgac cgttagcagt gggcgtgatt actgcgaaag aagcagtagt
     6781 agttgcaagc gggttaagtt t
//
LOCUS       DAOQKQ010000146         6796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_5589_length_6796_cov_5.927310, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000146 DAOQKQ010000000
VERSION     DAOQKQ010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 6796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6796
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5589_length_6796_cov_5.927310"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="PKZ49_09590"
     CDS             complement(<1..786)
                     /locus_tag="PKZ49_09590"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HND36855.1"
                     /translation="MTQANAYEQYMLELINIERAKVGSQPLAFDGDLNESAENHSTWM
                     IATDTFSHTGVGGSSPGDRMKTAGYIFSGSWSWGENIAWMSTRAPTGLQDEVLQLHTM
                     LMNSSGHRANILNNNFREIGVGFEVGQYQAYEGAFVTQNFAKTNTNPFLTGVAFDDRD
                     DDLRYDMGEGLSNFTVSAKNNTTGAITTTQTTLAGGYELELAAGNYTISFSAPGFTTT
                     TKQASISTKNVKLDLIDPVGSNTINGTSGPDTLQGTTNSDIIFG"
     gene            1275..1916
                     /locus_tag="PKZ49_09595"
     CDS             1275..1916
                     /locus_tag="PKZ49_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36856.1"
                     /translation="MIRDQFKEYPVAVSYKNIRAELDTGDIVVFGGQYRMSKIIRLVT
                     RFPASHVASIIRENDRISFIEASEGDMYPEREGVIISQFSNSIPFYKGDIWIARLSAE
                     IRRKADIGKMKAFLQEQVGKGYDYKNMPKAGFDFLDRIGITRSEENLSQMFCSELVAA
                     GLKAAGIFPEDINVSEVSPADIVKYKIFEPTYYQIKAFNYKAIKLPHFNTVVI"
     gene            complement(1940..2500)
                     /locus_tag="PKZ49_09600"
     CDS             complement(1940..2500)
                     /locus_tag="PKZ49_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HND36857.1"
                     /translation="MIRENLIKALIAIGVFVVLMAALGFLFEDELATATSWVVERIGF
                     AGLCMILLVTDTFVTPFPPDILLIVIAKSSLSEQWPFYVLILSMVSVFAGMLGWGIGR
                     WLGHFQFAKRLFGEFKDEHREFIRRYGFWAVVIGAITPLPFSVTCWASGVMGLRAVTV
                     FSAAALFRIPRFFIYYWIIASTSQWF"
     gene            complement(2589..2939)
                     /locus_tag="PKZ49_09605"
     CDS             complement(2589..2939)
                     /locus_tag="PKZ49_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HND36858.1"
                     /translation="MPPLLKKTTKLNLLGIINSILLLGLLYAFPAWSDNLEQLRASGM
                     IGENAGGFVVARDPSIQGEVEAINAKRKAIYQEKATAQGVSPEQVGKVYASEIFKKVP
                     AGTWIQLNGQWVKK"
     gene            complement(2968..3183)
                     /locus_tag="PKZ49_09610"
     CDS             complement(2968..3183)
                     /locus_tag="PKZ49_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HND36859.1"
                     /translation="MHNIIRLGDQVLAVASLSLFVMMIVACTPTVKVESPQEPITINL
                     NIKLDADIRVKLEEEAKKDIAANPDVF"
     gene            complement(3288..6446)
                     /locus_tag="PKZ49_09615"
     CDS             complement(3288..6446)
                     /locus_tag="PKZ49_09615"
                     /inference="COORDINATES: protein motif:HMM:NF023167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH domain-containing protein"
                     /protein_id="HND36860.1"
                     /translation="MNLLILSVAQCRILLPIPTVLIFFKLVKRLSIWFLIFCSGLALL
                     ASILYFSRYALLEALLIHQLQAKNVPLQSFTIRNISLNQVLIRDLSLGTHEELRIDQI
                     DINWSLIDLLKGKLDVVSLKGITIWLDFSGQQPVLGSLHPLLDSPSNDKNGPVTIQLP
                     AIAKLQADIDFRSAQGNLRLNLFGKINQREALTQSAVIEFGLSGFSIKTNGQFNVTID
                     STQTIQGLIAIADGSINRPDLEIAKISGETTFALLAMQLRQFKAELNLDEILFPESQQ
                     SVVQAYKQASLDLQLIENHIQLTGALLTTNNAPVLDIQITANKPQDSNDFDIAMNASG
                     RLSYLPWNLGGREQPSSGTFSLGLQGSGQIPSNVSQSGDSSWLEKIQFEGNAQLDCQA
                     LSYETKLLNLSGSLGFKVAINKGMGQANLTPDSIIQLGSADPAWLTQSGLPPALSKDL
                     AKGGTLRFNQTKQDQLSQVNWKLQSKQVDIRPQLFASLRFAKTRADFMGHGQVELKLN
                     ESKAAHGIIDSWNGNFNLSTQIDHMDLGSLTVHKMLMNIPMRTSACGKNWSVALTEPG
                     QIRFGLSDKTKQFSIKQPLHLVISQLDIDAKQRIDDFSIKHQVSATLSPFSAWMMSQI
                     EPIEIKIHPGRFALSGQLDKDQPYQAEGKLTGATALLPQSQIKLENIVASLSLGTKKR
                     ALAQLAIGKLYHLADAPFFAPHTLSATINDKTKGKQHIYTLDVSGGLPNLKYFHLKAE
                     QNLDNGFGKLKFNLTPIHFIPGGTQLSDLLPGLGQITDMQGVVHAHTQVDWSKKGVQK
                     SGGAIVADNLSFTHPTFSIAGLRTRIDFDQLLPINTPPQQQIHIKTIDAGIPLSDLNI
                     IYQIESNRNNQPRIKLEDTQLAILDGILTIEPVTIDPLSRDTKITITLNNINLKRFFD
                     FIQIEGLAGEGRLDGRVPILLTDSRITIDKSRLMAKSPGILRFQSEKASQMLANAGTE
                     MNLVLDVLEDFHYSELSLSIDKSAEHDLTATLSLLGQNPAVKEGQMIRLNINLASNLD
                     KILKAISLGYNVSNEILKDLFR"
     gene            6566..>6796
                     /locus_tag="PKZ49_09620"
     CDS             6566..>6796
                     /locus_tag="PKZ49_09620"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HND36861.1"
                     /translation="MQRFIGEAPEKIIDSSTTLFWLRRDLRLEDNAGLFHALNENSTV
                     LALFIFDTVILERLEDKKDARVEFILQSLLILK"
BASE COUNT         1937 a         1367 c         1462 g         2030 t
ORIGIN      
        1 accaaagata atatcgctat ttgtagtgcc ttgtaatgta tccggaccag atgtcccgtt
       61 gatggtgttg ctgccaacgg gatcgataag atcgagttta acattcttag tgctaatcga
      121 tgcttgtttc gttgttgtcg taaagccagg cgcagaaaag ctgatcgtgt agttaccggc
      181 agccaattcc aattcatatc ccccagcgag tgtggtttgg gttgtggtaa tcgcaccggt
      241 agtgttattt tttgcgctga cggtaaaatt gcttaatcct tcgcccatat cgtagcgcaa
      301 gtcatcatcc cgatcgtcga atgctacgcc tgtcaagaat ggattggtat tcgtcttggc
      361 aaaattctga gttacaaatg cgccttcata agcctgatac tggcctactt cgaatcctac
      421 ccctatttca cgaaaattat tattcaggat attagcccga tggcctgaag aattcatcag
      481 catggtatgg agttgtagca cctcgtcctg aaggccagtt ggtgcgcgtg tgctcatcca
      541 agcaatattt tctccccagc tccaagatcc agaaaaaata taaccagcgg ttttcatacg
      601 gtctccagga ctggaaccgc cgacacccgt gtgtgagaaa gtatcagtag cgatcatcca
      661 ggtactatgg ttctcagccg attcatttaa atcgccatca aaggcaagcg gttgtgagcc
      721 gaccttagca cgttcaatat tgatgagttc gagcatgtat tgttcataag catttgcttg
      781 agtcataact gaaatactcc tagatgaaat gctcgccaaa attgctatgc gagcgttaaa
      841 gaaagtagct cagtgcagta ccattgcacc agcactcaag ttatttgagt aaaataacct
      901 tagcagtaac tatgccagta ttttattttc tattagaaac aattagttga gtgattattt
      961 gataaattga ctgaaataag taatagtgat ttgacgcttc taggctacat accatcgata
     1021 agaaattcag taacagcttg ataagagaag agataatttt gtcactgatt cacaacaaac
     1081 tcatacagga tctcaggaag ctaagtattg cattattaaa acaataatga tgtgaccaac
     1141 atttataact aataccgatc aattatttga ttgccctgac gcaaataagg gtttgatttt
     1201 gactttgtat ttcatctttg atactttcca gtcatgctga tagtgttatt agcctaacaa
     1261 aaaagggggg aataatgatt cgggatcaat ttaaagaata tccagtggcc gtaagttata
     1321 aaaacatacg tgctgagctt gatactgggg atatcgtcgt cttcgggggg caataccgga
     1381 tgtcgaaaat tatccgtttg gtgacccgat ttcctgcttc ccatgttgcg tcgatcattc
     1441 gtgagaatga tcggatctca tttattgaag cttctgaggg tgatatgtat cccgagcgag
     1501 agggcgtcat tattagtcaa ttttcgaatt ccattccatt ttataaaggg gatatttgga
     1561 ttgcccgttt gtcagctgaa attcgaagaa aagctgatat tggaaaaatg aaagcgttct
     1621 tacaggagca agtcggtaaa ggctatgatt ataaaaatat gccgaaagcg ggttttgatt
     1681 ttctggatcg gatcggcatt acgcgaagcg aagaaaattt atcgcaaatg ttttgttcag
     1741 aactagttgc tgcgggttta aaggctgctg gtatttttcc ggaagatatc aatgtcagcg
     1801 aggtttcgcc tgcagatatt gtcaaatata aaatctttga accgacgtat taccaaatca
     1861 aagccttcaa ctataaggca attaaactgc cccatttcaa tacagtcgtt atttagttaa
     1921 cgaaatgtat ttacgttgat tagaaccact gacttgttga cgcgattatc cagtaataaa
     1981 taaagaaacg gggtatccgg aataaggctg ctgctgagaa aacggttact gcgcgtaaac
     2041 ccattacacc ggatgcccag catgttaccg agaagggtag cggtgtgata gcaccgatca
     2101 caactgccca aaaaccatat cgtcgaataa attcgcgatg ctcgtctttg aattcgccaa
     2161 ataagcgctt cgcaaattga aaatggccta gccaacgacc aattccccat cctagcatgc
     2221 cggcaaaaac tgaaaccata ctcagtatca gaacataaaa aggccattgt tcggaaagtg
     2281 agctcttcgc gattacaatg agcaagatat cgggaggaaa cggcgttaca aacgtatcgg
     2341 taacaagcaa gatcatgcac agaccagcga agccaatacg ttctacaacc cagcttgtgg
     2401 cagtagctag ctcgtcttca aataaaaagc cgagtgctgc catgagaacg acaaacacac
     2461 caattgctat caaagcctta atcaagttct cacgtatcat gcttgtttta ttgagtaaac
     2521 tgaattattg ggtaattgaa tgaattaacg gcatgaattc cagaacaacc tgagccgaac
     2581 gtaagcggtt atttttttac ccattgccca ttcaattgaa tccaagttcc tgctggaact
     2641 tttttgaaaa tttctgatgc ataaactttt ccaacttgtt cagggctaac accctgtgcc
     2701 gtagcttttt cttgataaat cgctttccgt ttggcattga ttgcctctac ttctccttga
     2761 atactgggat cacgtgctac aacgaaacca cccgcgtttt ctccgatcat tccggatgcg
     2821 cgaagctgct ccagattatc tgaccaagct gggaacgcat agagtaatcc taataatagg
     2881 atgctgttga ttattcctag caagtttaat tttgtggtct ttttaagtaa tgggggcata
     2941 tcattctcct tatcaagcgc aatatgttta gaaaacatcg ggattggctg caatgtcttt
     3001 tttggcttct tcttcgagct ttacccgaat atcggcatca agcttgatat tgaggttaat
     3061 cgtaatcggt tcttgtggcg actcgacctt cacggtcggt gtacacgcca cgatcatcat
     3121 tacaaacaaa gacagcgacg caaccgctaa gacttgatct cccaatcgta ttatgttgtg
     3181 catgattgca ctcctttaat aaagttatgc ttagagccgc atttcactcg aattgccgtg
     3241 agcatatcag actcatcgtt tttattgtag gtcgttcaaa cttaatctta tctgaacaaa
     3301 tcttttagaa tctcattgga aacgttataa cctaaactaa ttgccttgag aattttgtct
     3361 aaattactcg ccaaattaat gttgagtcgt atcatttgcc cttctttgac tgctggattt
     3421 tgaccgagta atgaaagcgt agctgttaga tcatgttctg ctgatttatc aatggataac
     3481 gataattcgg aataatggaa atcctctaac acatcgagga caagattcat ttctgtaccg
     3541 gcatttgcaa gcatctgtga ggccttttca gactggaatc gcaatattcc tggagattta
     3601 gccatcagac gacttttatc gatggtgatt cgactgtccg tcaaaagaat aggaactcgt
     3661 ccatccaagc gaccctctcc cgctagccct tcaatctgaa taaagtcaaa aaaacgcttg
     3721 agattgatat tattcaaggt aatggtaatt ttggtatccc ttgagagggg atcgattgtt
     3781 accggttcaa tcgttaaaat tccgtccaaa atagctaact gggtatcttc aagctttatc
     3841 cgaggctggt tattacggtt gctttcgatt tgataaataa tgttcaaatc acttaatgga
     3901 ataccagcat cgatggtttt gatatggatt tgctgctgag gcggtgtgtt gatcggtaat
     3961 agctggtcga aatcaatgcg agttctcagt cctgctatgc taaaagtagg atgggtaaac
     4021 gatagattgt ctgcgacaat agcaccaccg cttttctgaa ctcctttttt agaccaatcg
     4081 acctgagtat gggcgtgaac cacgccttgc atatcggtta tttgtccaag ccctggaaga
     4141 agatctgata actgagtgcc cccaggtata aagtgaatgg gagttaaatt gaatttaagc
     4201 ttaccaaagc cattgtccag attctgttct gctttgaggt gaaaatattt tagatttggt
     4261 aaaccacccg atacatcaag tgtataaatg tgttgctttc ccttcgtttt atcgttaata
     4321 gtcgctgaga gggtatgtgg tgcaaagaat ggagcatctg caaggtgata tagcttgcca
     4381 atcgcgagct gcgcaagagc gcgtttcttg gtgccaagcg aaagggaagc cacaatattt
     4441 tctaatttga tctgagattg agggaggagt gccgtagcac cagtcaattt tccttcagcc
     4501 tgatagggct ggtctttatc cagttgccca cttaaagcaa atcttcctgg gtggattttt
     4561 atttcaatcg gctctatttg cgacatcatc caggctgaaa atggcgaaag ggtggcgcta
     4621 acctggtgtt ttatcgaaaa gtcatcgata cgctgtttcg catcaatgtc tagttgagaa
     4681 atgactaaat gcagtggttg cttgatagag aattgtttag ttttatcgct aagaccgaat
     4741 cgaatctgac ccggttcggt taaggcaaca ctccagtttt taccacaagc tgaggttctc
     4801 atcggaatat tcatgagcat tttatggacg gtcagtgatc ccaaatccat atgatcgatt
     4861 tgggtggata agttaaaatt gccattccaa gaatcgatta ttccgtgcgc tgcttttgat
     4921 tcattcagct tgagttcaac ttgtccatgg cccataaaat ctgcacgtgt ttttgcaaaa
     4981 cgaagtgagg caaaaagctg aggcctaata tcaacctgtt tagattgtag tttccagttg
     5041 acctgggaaa gttgatcttg tttggtttga ttaaatcgca gcgttccgcc tttcgctaaa
     5101 tccttactca aagcaggcgg taagccggat tgagttaacc aagctgggtc tgcactgcct
     5161 aattgaataa tgctatccgg tgttaaattg gcttgaccca ttcccttatt tattgcgact
     5221 ttaaaaccca ggctgccgct gagattcaat agcttcgttt catagcttaa agcttgacaa
     5281 tcaagctgtg cattgccctc aaactgaatt ttttctaacc agcttgaatc accggactga
     5341 gatacattgg aaggaatctg acctgatccc tgtaatccca aggaaaatgt ccctgagctt
     5401 ggttgctccc taccccctag attccaaggt aggtagctta gtcgcccact cgcattcatg
     5461 gcgatatcga aatcattact atcttgcggt ttgttggcgg ttatttgaat atctagcact
     5521 ggcgcattat tagtagttaa taatgcgcca gtgagctgaa tatggttttc tataagttgg
     5581 agatcaagcg atgcttgctt atatgcttgc acgactgatt gctgactttc agggaacaaa
     5641 atctcatcta aatttagctc agctttgaac tgacgaagct gcatcgctaa taatgcgaac
     5701 gtagtttctc cgctaatttt agcaatctcc aaatctggcc gattgatcga accgtcagcg
     5761 atggcaataa gaccttggat tgtttgagtt gaatcgatag taacgttgaa ttgcccgttt
     5821 gtttttatag aaaaaccact caatccaaat tcgatgacgg ctgactgagt tagggcctcg
     5881 cgttgattaa tcttgccaaa taagttaagg cgtaaatttc cttgagccga gcgaaaatca
     5941 atatccgctt gcagttttgc gattgcagga agttgtatag ttacagggcc gtttttatcg
     6001 ttactggggg aatcaagcag aggatgaagt gaccctagca cgggttgctg accactgaaa
     6061 tcaagccaaa tggtaatgcc ctttaatgaa acaacatcga gttttccttt gagaagatca
     6121 atgaggctcc aattaatatc aatctgatcg atccgaagtt cttcatgggt gccgagtgat
     6181 aaatctcgta tcaatacttg attgagagaa atattgcgaa ttgtgaatga ctgcagcgga
     6241 acatttttgg cttgtaactg atgaattaac agcgcttcga gtaaggcata gcgagaaaaa
     6301 taaaggatgc tcgcgaggag tgcgagtccg ctacaaaaga ttaagaacca gatgcttagt
     6361 cgtttcacca gtttgaaaaa aatcagtacg gttggaattg gtaatagtat gcgacattgt
     6421 gctacgctta aaatcagcag attcactgca ttccgtaaat aaaggaatga aaccattaat
     6481 aacatgaagt aatcgttatc gcaattttgc ttggcaaaac tcagctcacc cggtattgcg
     6541 atgatttgat attaaaggaa atacattgca acgtttcatc ggagaagctc ccgagaaaat
     6601 cattgattca tctacaacac ttttctggtt gcggagagac ttgcgtcttg aagataacgc
     6661 gggtttgttt catgcgctca atgaaaattc aaccgtatta gcgctcttta tttttgatac
     6721 cgtgattctt gaaaggttag aagataagaa agatgctcgc gttgaattta ttttgcagtc
     6781 acttctaatc ttgaaa
//
LOCUS       DAOQKQ010000147         6680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_5737_length_6680_cov_5.110943, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000147 DAOQKQ010000000
VERSION     DAOQKQ010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 6680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6680
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5737_length_6680_cov_5.110943"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..2739
                     /locus_tag="PKZ49_09625"
     CDS             229..2739
                     /locus_tag="PKZ49_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HND36862.1"
                     /translation="MNLVLLSLKMLRRDWRAGELRILALALIIAVSSITTVGFFADRV
                     QLALAQESNRLLGADLVVTSDRPIPIEFATQAEHLQLKIVNLIRFPSMVSKGDHNLLA
                     EIRAVSPGYPLRGELKMMDRPNENATEENIYLAEGIPTQGTIWIDEKLLLGLETALDE
                     KLEVGIAEMTVTSIVAREPDHSVGFINMGPRLLMNIEDLAETQLIQPGSRVTYQLLVA
                     GKESDVAQFRDWVQPKLVQGQRVEGIRDARPEIRAALERAEKFLSLAALVSVIVAAAA
                     IALSSRRFIQRHLDGCAVMRCLGASQTTLFSLYFYHLVLFGLVASTIGCVLGLIAQEF
                     LSNWMAELVNSSLPWPSIVPAIYGLVMGVVLLLGFSLPPLLNLKSVPALRVIRRDMGS
                     VNLHSLTGYSLGLLVLALLFIWEAGDLRLGLMVVLGFIGAIAVFSLLGWLLIQSLLLA
                     RRHNTNAWGYGLANIRRRIVTSVVQAIALGLGLMAMLVLTVLQNDLLKDWQMSLPPET
                     PNRFLLNIQTDQLTSLTKFFDQHEMPEPTWYPMVRGRLTAINGHPVQVENYKDIRKKQ
                     LLNREFNLSWSNELQSDNEITDGRWWSENQPMTESEISLEEEIARVIGVNIGDRLTFN
                     IADQLFEATITNLRKVDWDTFRVNFFAIARPGYLENYPTYYVTSFHLPADKTKLLHEL
                     IREYPNLLIIDVASVIEQIQKMIFQVSRAIEFVFIFTLLAGFVVLYAAIVATQDERVY
                     EAAIFRTLGAKRNQLVRAWASEFMILGALVGIFAAAGATVLGYVIGKHALHIPYSFNP
                     WIWLIGLLIGMVGVMVAGLIGTRFTLAQPPLLTLRRLG"
     gene            complement(2796..3692)
                     /locus_tag="PKZ49_09630"
     CDS             complement(2796..3692)
                     /locus_tag="PKZ49_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36863.1"
                     /translation="MLQLNGELNMKRQAKRLILSFCALGLLSNSYIALADTTSDTETL
                     LNWAEKTFPQFFKSKQATQSIEPWLYRFYPETGIYAGVNLTDNNVYTLGGPWGNNPAL
                     IDSLSNLIAQINNSGGNGNIASCDTTNTIPGITYSQSGNVITVTSNGQCVTAPEQNAT
                     NLCPIPKQTTPTGISILSTNTVTRSQISGITASIPGLLDPFLNLISASSNAKHCTVNA
                     PNENTNLIVNSDLCFDITTQLSDLIGVIEGITVNPPITLSTAGTYTSQAVSDCFATDA
                     ATITDAVTGDLWVKENGSFVKK"
     gene            3900..4508
                     /locus_tag="PKZ49_09635"
     CDS             3900..4508
                     /locus_tag="PKZ49_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HND36864.1"
                     /translation="MTDPIEIRDAIIDTAVTLAAQKSWEAVRLYDVATALNLSLDQIR
                     PYFHEKEDLVDAWFDRADSAMLKQAEAAHFYELSVEQRIFQLIMGWLDALSSQRKVTR
                     QMILGKLEFGHLHIQIPGIMRISRTVQWIREAAHRDATSVQRALEESALTTIYLMTFI
                     YWMQDDSLGSTRTQQFLKQRLQQAFWVGELFFAKHHTDSSRA"
     gene            4562..5605
                     /locus_tag="PKZ49_09640"
     CDS             4562..5605
                     /locus_tag="PKZ49_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HND36865.1"
                     /translation="MDPLTHALSGVLIVRAVEPILPSLKLLPLRVRVVAGLVAAAFPD
                     IDIALRLIDTLIYLNWHQGPTHSLVLLPIWAFLLAHAFAWIYRGRFAWTLFYTPACLG
                     LAIHILGDWITAYGLMVFAPFSTERFFVPLAFVIDPWITGMIVAGLVMSNVFPRQTYI
                     AACALLCLALYGLLLTDKHDQAVKIGRDYVKNHSILDASVAVLPQPFSPFNWKIIVSE
                     NEKYHVLMVKLSDQSVFIPDMGLLSKMASAYTPVSTARWQKINRYGDVSAHAKLVREA
                     WFDPIFDDFRQFAVFPQFDKMDVYESNICVWFFDLRFQFPLLPPSFRYGVCRDKMGST
                     WTIERHRGIFWID"
     gene            5648..>6680
                     /locus_tag="PKZ49_09645"
     CDS             5648..>6680
                     /locus_tag="PKZ49_09645"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing response regulator"
                     /protein_id="HND36866.1"
                     /translation="MIDKSTIKILILDDEPFMLKLLARMLANQGFVHVVCCNNGNDAL
                     AQLNVESTRPDLILLDLNMPEMDGIEFIRYLVERQFTGSLILVSGEDERMLKTTERLV
                     QAHKIAVLGYLQKPVKPDALLAMLAQWSPPSKAAPTKPRKTYSADDIRLAIVRGELVN
                     YYQPKVSVATGTVIGVETLVRWQHATDGLVFPNQFITVAESSGLINELTRLVLLNAFN
                     QAKRWQQSGMDLRVAVNVSMDNLASLDFQDLVVDLATQVGIPPNKIVLEVTESQLMRD
                     IRVSLEILTRLRLKRFHLSIDDFGTGHSSLAQLRDIPFDELKIDQGFVHQAWRDDTLR
                     VIYDASLALA"
BASE COUNT         1776 a         1237 c         1577 g         2090 t
ORIGIN      
        1 ttgttgtgga aattttcaaa gcattattat ggtgtctccc taagactgtc aatagttaag
       61 aaaatgtttt acgcactgcg cttttctcga attcaccgat catttgattg ctcagggcag
      121 gcagttttaa tgctgtgatt aaaatcgaga tcgatctggg gttaaatttt ttcaatttcg
      181 agtgattaaa gcactgtttg tatgaccaaa attaatggat gttaaagaat gaatctcgtt
      241 ttactttcgt taaaaatgtt gcgtcgtgat tggcgcgcag gtgaattaag gattctggca
      301 ttagcgctga ttatcgcagt cagtagcatt actacggtag gtttttttgc tgatcgtgtg
      361 cagcttgcgc tagctcaaga aagcaatcgt cttttaggcg ctgatttagt tgtaaccagt
      421 gatcgaccca ttccaatcga gtttgcgact caagccgagc atcttcaact aaaaattgtg
      481 aatttgattc gttttccgag catggtaagc aaaggagatc ataacctgct agccgaaatc
      541 agagcggttt cgccaggata tccattgcgt ggggaattga aaatgatgga tcgtcctaat
      601 gagaatgcaa cggaggagaa catctatctg gcagaaggga ttccaactca gggtacgatt
      661 tggatcgatg agaaattact tttagggtta gaaactgcgc tggacgagaa attggaagta
      721 ggtattgctg aaatgaccgt cacatcgatc gttgcacgcg aacccgatca ttcagtcgga
      781 tttatcaata tggggcccag attattgatg aatattgaag atttagctga aacccagtta
      841 attcagcctg gaagtcgagt gacttatcaa ttgctggtgg caggtaaaga gagtgatgtc
      901 gcccaattta gagattgggt acaacctaaa ttggttcaag gtcaacgtgt agaaggtatc
      961 cgcgatgccc gacccgagat acgtgctgcg ctggagcgag ctgaaaaatt tcttagcctt
     1021 gctgctttgg taagtgtcat tgtcgccgct gcagccattg ctttatcttc tcgacgcttt
     1081 attcagcgtc atttagatgg atgtgctgtg atgcgttgtc taggggcgag tcagacaact
     1141 ttgttcagtc tttattttta ccatttggtg ttgtttggcc tggttgcaag tacgatcggg
     1201 tgtgttttgg gattgattgc tcaagaattt ttatcaaatt ggatggctga attggttaat
     1261 tcatcacttc cttggccaag tatcgttcct gctatttatg gattggttat gggagtcgta
     1321 ctattgctgg gtttttcatt accaccgtta ctcaatttga aaagtgtacc tgcattgcga
     1381 gtcattcggc gtgacatggg gagtgttaat ttgcatagtt tgaccggcta tagtttggga
     1441 ttattggtgt tagcgctttt gtttatctgg gaagctgggg atttgcgtct agggctgatg
     1501 gtagtacttg gttttatcgg tgcaattgca gtttttagct tgctgggttg gttactgatt
     1561 cagtcattgt tgctcgctcg gcgacacaat accaatgctt ggggctatgg gcttgctaac
     1621 attcgcagac gaattgtgac cagtgtggtg caagcaattg cactaggatt aggcttaatg
     1681 gcgatgctgg tattgaccgt gctgcaaaat gatctgttaa aagattggca gatgagtctt
     1741 ccgcctgaaa ccccgaatcg gtttttgctc aatatccaaa ctgatcaatt gacatcattg
     1801 actaaattct ttgaccaaca tgaaatgcct gaaccaactt ggtatcccat ggtgagaggg
     1861 cgattgacag caatcaatgg acatccggtt caagtggaga attataaaga tatccgaaag
     1921 aagcaactgc tgaatcgtga attcaatctt tcctggtcga atgaactcca gtctgataac
     1981 gagattacgg acgggcgctg gtggtctgaa aatcagccga tgactgagag tgaaatatcg
     2041 ctggaagagg aaattgctcg tgtcattggc gtgaacattg gtgatcgttt gacttttaat
     2101 atcgcagacc agttgttcga agcaaccatt accaatctac gtaaggtcga ttgggataca
     2161 ttccgggtca atttttttgc gattgctcgg cctggttact tggagaatta cccaacctat
     2221 tacgtaacta gcttccattt gcctgctgac aaaaccaagc tacttcatga gttaattaga
     2281 gaatatccta atttgctcat tattgatgtg gcgtcggtaa tcgaacaaat tcagaaaatg
     2341 atctttcaag tgtcaagagc gattgaattc gtgtttatct ttactttatt agctggattt
     2401 gttgttctct atgctgcgat tgttgcaacc caagatgaac gtgtatatga agcagcaatt
     2461 tttcgtacct tgggtgcaaa gcgcaatcag cttgttcgcg cttgggccag cgaatttatg
     2521 atactgggtg ctttagtcgg tatttttgct gcagcgggag ctacggtatt aggctatgta
     2581 attggtaaac atgcgctgca tattccgtat agctttaatc cttggatttg gctcatcggg
     2641 ttattaattg ggatggttgg ggtgatggtt gcagggttga ttgggacacg gtttacgctt
     2701 gctcaaccgc cattattgac attgcggcga ttaggataat gatcccatcc atcaatcagc
     2761 gcttgatgga tgggtgcgtg aattaattaa tagcgttatt ttttaacaaa actaccattt
     2821 tccttaaccc aaaggtctcc tgttactgca tcagtgatcg ttgctgcatc agttgcgaaa
     2881 caatcggaaa cagcttggct ggtgtaggtt cctgcagttg acaatgtaat cggtggattt
     2941 acagtaattc cttcaatcac accaatgaga tcagaaagtt gcgtggtaat atcaaagcaa
     3001 aggtctgagt tgacaatgag attggtgttt tcgttgggtg cattcaccgt acagtgtttt
     3061 gcatttgaag aagcactaat tagatttaaa aatggatcca atagtcctgg aatcgaagct
     3121 gtaattcctg aaatttgcga acgagtcacc gtgttggtgc taagaattga tatgccggtt
     3181 ggtgtcgttt gcttgggaat ggggcataga ttagttgcat tttgctccgg agcagtaaca
     3241 cattgaccgt ttgatgtgac cgtaatgaca ttgccactct gactataggt aatcccagga
     3301 atggtattgg tcgtgtcaca acttgcaata tttccatttc cgcctgaatt attaatttga
     3361 gcgatgagat tcgataagga atcgatcagc gctggattgt ttccccatgg accccctagt
     3421 gtataaacat tattatcggt taaattgaca ccggcgtaga ttcccgtttc tgggtagaag
     3481 cgatacaacc agggttcaat actctgtgtt gcctgtttgc ttttaaaaaa ttgagggaag
     3541 gttttttccg cccaatttaa caaggtttcg gtatcactgg tagtatccgc gagtgcgata
     3601 taactgttac tgagtagacc cagagcacaa aaagataaaa tgagtcgttt ggcttggcgt
     3661 ttcatattta gttctccatt taactgtaac atgtaaatct cgatttgatt ctaatagaaa
     3721 aatatcgact atgttcgcat ttacataaaa aaagcaagcg agtgatttaa atggttaaaa
     3781 cgatatattt tctgcatttg ctagataaaa tagcaacaat acaaatacag tccagtcgaa
     3841 ttgattgttg aattataaga aaaagtgggc ttactaatgg cttatttgcg gagactaaga
     3901 tgacagatcc gattgaaatt cgtgatgcaa tcattgatac agcagtgaca ctcgccgccc
     3961 aaaaatcatg ggaagccgtg cgtttatatg atgtagcaac tgctctaaat ctttcattgg
     4021 atcagatacg tccctatttt cacgaaaaag aagatttggt cgatgcttgg ttcgatcgtg
     4081 ctgatagcgc aatgttaaag caagctgaag cagctcattt ttatgagcta tccgttgagc
     4141 aacggatttt tcagttaatc atggggtggt tagatgcttt gtctagccaa cgtaaggtca
     4201 cacgacagat gattctgggt aaactggaat tcgggcatct tcatatccaa attccaggaa
     4261 taatgcggat tagccgaaca gtacaatgga ttcgtgaagc agcgcatcgc gatgcaactt
     4321 ctgtgcagcg agcattagaa gagtcagcat tgacaacgat ttatttgatg acttttattt
     4381 attggatgca ggatgattcc ttagggtcga cgcgtactca gcaatttcta aagcaaagac
     4441 ttcagcaagc tttctgggtt ggtgagcttt tttttgcaaa acatcatacc gattccagtc
     4501 gagcatgatc tgacttatca aaatatcgcg taatgataga acgaactcat catactttaa
     4561 gatggaccca cttacgcatg cactaagcgg tgtactcatc gtgcgagcag tggaacctat
     4621 actgcccagc ttaaaattat tacctttacg agtacgagtt gtagctggcc tggtagcagc
     4681 agcattccca gatatcgata tcgcattacg acttatcgac acgctcattt atttgaattg
     4741 gcatcaagga ccgacgcatt cgttagtatt gttaccaatc tgggcatttt tattagcaca
     4801 tgcttttgct tggatttacc gtggccgatt tgcttggaca cttttctata caccggcttg
     4861 cttagggtta gcaatacata ttttaggaga ctggattact gcttatggtt tgatggtgtt
     4921 tgctcctttc agtacagaac gtttttttgt tccactggca tttgtaatcg atccgtggat
     4981 aacaggaatg attgtcgcag ggcttgttat gagcaacgtg tttcctcgcc aaacttatat
     5041 cgctgcttgt gcgctgttgt gcctagcgtt atatggatta ttgttgacgg acaagcatga
     5101 tcaagcggta aaaataggaa gagactatgt caaaaatcat tcgattctcg atgcttcagt
     5161 tgcggtatta ccacaacctt tctcgccgtt taactggaaa atcattgttt ctgaaaatga
     5221 gaaatatcat gtgttaatgg tgaaactctc cgatcaatcg gtatttattc ctgatatggg
     5281 gttactttca aagatggcga gtgcttacac tcccgtttca acggcacgtt ggcaaaaaat
     5341 taatcgctat ggagacgttt cagcgcacgc aaagttagtt cgggaagcct ggtttgatcc
     5401 gatttttgat gattttcgtc aatttgcggt atttccacaa ttcgataaaa tggacgtgta
     5461 cgaatcgaat atctgtgtgt ggttttttga tttgcgcttt caatttccgc tattgccacc
     5521 ttcatttcgc tatggtgttt gtcgggacaa gatgggttca acttggacca tagaacgcca
     5581 tcgaggcata ttctggattg attagatacc ttctttagct gactgagtca caccgaaaag
     5641 gaataatatg atcgataaat ctacgatcaa gatactgatt cttgatgatg aaccctttat
     5701 gctgaaatta ttggcccgta tgctagccaa tcaagggttc gttcacgtgg tttgctgtaa
     5761 taatgggaat gatgccttag cgcaacttaa tgttgaatcg acacgacctg atttgatttt
     5821 attagatcta aacatgccgg aaatggatgg cattgaattc attcgttatc ttgttgagcg
     5881 tcaatttacc ggtagtttaa ttcttgttag cggtgaagac gaaagaatgc taaaaacgac
     5941 tgagcggctc gtgcaggcgc ataaaattgc tgtactcggt tatttacaaa aacccgttaa
     6001 acccgacgcg ttacttgcga tgctagcaca gtggtcacca ccctcaaaag ctgctccgac
     6061 taaaccaaga aaaacctata gcgcagatga cattagatta gctattgtga ggggtgaatt
     6121 ggttaattat tatcaaccta aggtttcagt tgctacaggg acagtgattg gagtggaaac
     6181 attggtgcgt tggcagcatg ctaccgatgg tttggtattt cctaatcaat ttattacagt
     6241 agctgaatcg agtggattga ttaatgagtt aacgcggtta gttttattaa atgcatttaa
     6301 ccaggcgaag cgatggcaac aatcgggaat ggatttacgt gtagcggtaa atgtatcaat
     6361 ggataatttg gcatcgctag atttccaaga tcttgttgtg gatctggcta cccaagttgg
     6421 cattccaccc aataaaatcg tattggaagt cactgaaagc caattgatgc gcgacattcg
     6481 ggtatcactt gaaattttga cccgtttgcg tttgaaacgg tttcacttgt cgatcgatga
     6541 ttttgggact gggcattctt cacttgcaca gttgcgcgat attcctttcg atgagctcaa
     6601 gatcgatcaa ggctttgttc atcaagcatg gcgagatgac actttgcgtg tgatctatga
     6661 tgcaagcctt gctttagcca
//
LOCUS       DAOQKQ010000148         6675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_5746_length_6675_cov_5.119637, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000148 DAOQKQ010000000
VERSION     DAOQKQ010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 6675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6675
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5746_length_6675_cov_5.119637"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            337..2190
                     /locus_tag="PKZ49_09650"
     CDS             337..2190
                     /locus_tag="PKZ49_09650"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HND36867.1"
                     /translation="MQKVIVKHMTGSKANQTEIFELPIDAITFGRDTNCKVAYDPLKD
                     DLVSRTHLKITLVEDDQFLLTDLGSSNGTFVNEQKIAKPVILQAGDIVQLGKNGPKFE
                     FDLDPRPPVAAKATRLQEVPIPKSTRESQVSSTVESQLSRTDIQNISTNQKQGVGRET
                     VERLIDRVESDARVKIAETEGRARRKMINVSAGIFTVIVLVAGYFFYQNQQSQKQLQA
                     TEEQLKSNIAAAKEEQSRQMEEMKSNTPMSATEISQKYESAVVYIETSWKLKDVESGQ
                     QLYHGRVCQKSKSEKRCLPAYVSFQREGKEYVEPLLFTENVRDELFSSFGIETERGVA
                     IGKSNTGSGFVVDMNGFILTNTHVAAAWEVAYDISQLENGFVIRCNDEGKNCKVKGNI
                     ADTKYLRLLEKWIPANTQYLGGRPQTGKYVEGYHDYLEVTFPGLGQRSNARLTKISGN
                     ADVALIKVDAMKKLVHVELNSGRSISTGEPVTVMGYPIISPDTSVTRQNRKVPEPTIT
                     TGIIGKVIENKAGVTLDTVGKNVEMILFKGLNREDFLQKHMIHKEDGSLLVNELLYQL
                     TINAAGAGTSGGPVFDSKGQVIGIFTDLIRDEQGTQVSLALPIKYGLEIMK"
     gene            2280..3068
                     /locus_tag="PKZ49_09655"
     CDS             2280..3068
                     /locus_tag="PKZ49_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HND36868.1"
                     /translation="MASYFHLEIGSLSDTGRCRSVNQDCLGIDGFDSSHNFLAIVADG
                     MGGHQAGDIASRIAVDEIKHHYFVLLKKQSPDQALRQAFQEANTAILTRAQQFPEYQR
                     MGTTLVVLSLNKSAAYFASTGDSRLYLIRENAIQQLTRDHTLVAEMLRKGLLSVEQAK
                     NHPDKHIITHAVGTQEKVFVDTSDLPLLLKYQDCFLLCSDGLYDLVSDAEMLDYATQY
                     PVQQACQKLVDLANTRGGYDNISVIIIKILEKPGLTNEIPITQS"
     gene            3101..4480
                     /locus_tag="PKZ49_09660"
     CDS             3101..4480
                     /locus_tag="PKZ49_09660"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HND36869.1"
                     /translation="MDQIGHYKIIKKIGEGGMGEVYKGVDTMLEREVAIKLMRSELSN
                     RPDIVERFRSEAIALGRLNHSNIAAVYNFGQVESQYYMALEFVSGKTLDSIIKKLGKV
                     PWREALNYAIAALDGLEHAHQLKIIHRDIKPANIMINEQGNVKIMDFGIARILGKERM
                     TQIGRFIGTLEYMPPEQIQGKEADARSDVYAIGAVLYEMLTGHILFERDTEFELLNSQ
                     ISEKPKPLREFNHEVSPELEKIILRALEKEPGKRYASAREFSEVIRRLLLAEPSASLT
                     HQNSLIAFCKDYPAVAIAASLLLPVGSYIAWQLNFADPSQVSQSVVFPVEKLVVEPTQ
                     RAIVENQSIEAERITAITSEKIPEMRNSLQSFPPNLPSSGNVDIGDSTVEDTFDIESE
                     LMHVLPMPERKPEVPEYFVQTNKQSAKQISTSAQQNIVTKTKPSSSASDKPVDDPRID
                     EWAKDFFEN"
     gene            4524..5135
                     /locus_tag="PKZ49_09665"
     CDS             4524..5135
                     /locus_tag="PKZ49_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36870.1"
                     /translation="MKRLIIVPLAATAILSGCAHTTNLSKEGQAGIGAGLGGALGAAI
                     GYAVCGAECAAIGAGAGIVAGGLTAYALASDPYTQSAAQQAESWKKETGSEAEVVKVS
                     DVTENGQKRQRLDVQKMQLSDAQVVTKDRLSPRIKEQLINTKSSALKTGGYVHVVCPA
                     NAPSSVMSDIDKIGIGYSKDTTTASGYTIYLSRDRAELKSLNI"
     gene            complement(5149..5973)
                     /locus_tag="PKZ49_09670"
     CDS             complement(5149..5973)
                     /locus_tag="PKZ49_09670"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HND36871.1"
                     /translation="MADIFLSYASSNRTNAETLAIVIQKLGWTVWWDRQIPIGLTYDE
                     VIEKNLKAAKCVIVLWTKASAASDWVKNEASEGAKRKILIPILLENIEIPLEFRRNQT
                     AKLIDWRSATQYHPEFPKLYTRLTELLGHPRYSPEGPPFKEFNHQPSIVIEAPPPPPW
                     RKFFIVAAVILSLVVIVPPLVNIVNPPPTTSSSAQIDELPPLNMSAEQSYQKGKQLFD
                     SKKYKEAFIWLEAAAHQDHIGAINKVIEMYSDGKGVDENYGKVAEWVEKKSFLHKE"
     gene            6138..>6675
                     /locus_tag="PKZ49_09675"
     CDS             6138..>6675
                     /locus_tag="PKZ49_09675"
                     /inference="COORDINATES: protein motif:HMM:NF024908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HND36872.1"
                     /translation="MKRTEWLQETRKMRFEEAYEGYRSKSLTQREAALLLGVCDRTFR
                     RYMDRYEEGGLGALLDKRLTQASHRCAPIDEVMQLTQQYRKDYSGWNAMHFHAWYRRD
                     GGTRSYTWVKNQLQEAGLIKRSTKRGAHRMRRERSPLPGMMIHQDGSTHEWVVNQKWD
                     LIESLRVFRRPFSLSQATL"
BASE COUNT         2105 a         1205 c         1518 g         1847 t
ORIGIN      
        1 gtgcagaaaa tcaaaattga ttaaaccgac tattatttta gctgagttga agccattgaa
       61 ggagagcgaa gagaaaagtc gcggaaccat tcacaaaacg atgtacgaaa aaattatcgt
      121 catgtgaatt ctgcattacc gatgtgtttt ttatcacagg aagtgaagtt ttgctacatt
      181 aaaatgacaa aaaactatct gtgataagtg gcaaaaagaa attgaattga tagaatgaaa
      241 gatgagtgaa agtactatct tttttgatat ctatataaga aagtagattg aaatctgata
      301 accacaacgt gatttttcta tgaagggaat gcaatcatgc aaaaagtaat tgttaagcat
      361 atgacgggtt ctaaagcaaa tcaaactgag atttttgagc ttcctatcga tgcaataacc
      421 tttggtcgag ataccaattg caaagttgcg tatgatccgc tgaaagatga tctggtaagt
      481 cggacgcatt taaaaatcac cctggttgaa gacgaccaat tcttactgac ggatttgggc
      541 agtagcaacg gcactttcgt gaatgagcaa aagattgcaa aaccagtcat attacaagct
      601 ggagatattg tgcaacttgg caagaacggt cccaaatttg aatttgatct tgatccgcgc
      661 cctcctgttg ctgcaaaagc aacgcgactg caagaagttc ccattcctaa atcaacgcgt
      721 gaaagtcagg ttagtagcac ggtagagtct caattaagta ggacagacat ccaaaacatt
      781 agcactaatc agaagcaagg ggtaggccgt gaaacggtag agcgactcat cgatagggtt
      841 gagagtgatg cgcgagtcaa gattgccgag actgaaggta gagcacggcg caaaatgatt
      901 aacgtcagtg cggggatctt cactgtcatt gtcttagttg ctggatattt tttttatcaa
      961 aatcagcaaa gtcagaaaca attacaggct actgaagaac aattgaaaag taatattgct
     1021 gctgctaaag aagagcaatc caggcaaatg gaggaaatga agtcaaatac cccaatgtca
     1081 gcaactgaaa tatctcagaa atatgaatcc gctgttgttt atattgaaac gagctggaag
     1141 ctaaaagatg tcgaatcagg tcaacagctc tatcatgggc gagtttgtca aaaatctaaa
     1201 tccgaaaaga gatgtcttcc tgcctacgtt tctttccaac gagagggaaa agaatacgta
     1261 gaaccgctct tatttactga aaatgttaga gatgaattat tctctagctt tggtattgag
     1321 actgagagag gggtcgcaat tggtaaaagt aacacaggca gcggttttgt tgtggatatg
     1381 aatggattta tcctaactaa tactcatgtc gctgcagctt gggaagtagc atatgatatt
     1441 agccaattag aaaatgggtt tgttattcgc tgcaacgatg aaggtaagaa ttgcaaagtc
     1501 aaaggtaata ttgcggatac caagtatctc agattgctag aaaaatggat tcctgctaat
     1561 acacagtatc taggtgggag accccagaca ggaaaatatg ttgaaggata tcatgattat
     1621 ttagaagtaa cttttccagg attaggtcaa cggagtaatg ctcgtttaac aaaaatttct
     1681 ggaaatgcgg atgtggcgct catcaaggtt gatgcgatga aaaaactggt tcatgttgaa
     1741 ctcaacagtg gtagatcaat ttcgacaggt gaaccagtta ctgtgatggg ttatcctata
     1801 atttctcctg atacctccgt aacaagacaa aatcgaaaag tacccgagcc tacaataaca
     1861 acaggaatta ttgggaaggt aatcgagaat aaagcaggcg taacattgga tacggtagga
     1921 aaaaatgtag aaatgatcct ttttaagggc ctaaataggg aggattttct tcaaaagcat
     1981 atgattcata aagaagatgg atcacttctt gttaatgaat tattgtatca actgaccatt
     2041 aatgcagctg gagctggtac aagtggcggt ccagtttttg atagtaaagg acaggttatt
     2101 ggcattttta ctgatttgat aagagacgag caaggtactc aagttagtct tgctttaccc
     2161 ataaaatatg gccttgaaat catgaagtaa tcttttactt atagctggac gtgatggagt
     2221 tttttgacag atggaaaaaa ttttttcaag attctcctga ttctgagcag aatcaacaaa
     2281 tggcatcgta tttccatttg gaaatcggtt cgttatcaga cacggggcgt tgccgatctg
     2341 tcaatcagga ctgtcttgga attgatggct ttgatagcag ccataatttt ctggctattg
     2401 ttgcggatgg aatggggggg catcaggctg gcgatattgc aagccgtata gcggttgatg
     2461 aaatcaagca tcattatttt gtgcttctaa aaaaacaatc gcctgatcaa gctttacgcc
     2521 aagcgttcca agaagcaaat actgcaatcc taacgcgcgc gcagcagttc ccagaatatc
     2581 aaagaatggg cacaacgctc gttgtgctat cactgaataa gtctgcagcc tattttgctt
     2641 ctactgggga tagtcggctt tacctaattc gagaaaatgc tattcagcag cttactcggg
     2701 atcatacttt ggtagctgaa atgctaagga aaggattatt aagcgtagag caagcaaaaa
     2761 atcatccgga taaacacatc attactcatg ccgttggtac tcaggaaaag gtttttgtcg
     2821 atacttccga cttaccatta ttattaaagt atcaggattg tttcctgttg tgttcagatg
     2881 gcttgtatga tttggtaagc gatgcagaga tgcttgatta tgcaacgcaa tatcctgtgc
     2941 aacaggcttg ccagaagctg gtagatttag cgaatacccg aggtggctat gataatattt
     3001 cagtaattat cataaaaatt ctggaaaaac caggactaac taatgagata ccaattaccc
     3061 aaagctaaca aaattcaaaa tttttaaaag atattgcatt atggatcaaa ttgggcatta
     3121 caagattatc aaaaaaattg gcgaaggagg tatgggagaa gtctacaagg gtgtggatac
     3181 tatgctagag cgcgaggttg caatcaaact catgcgttca gaactaagta atcgcccaga
     3241 cattgtcgag cgtttccggt cagaagcgat tgcattaggt cgtctcaatc attccaatat
     3301 tgcagcagtt tataattttg ggcaggtgga aagtcagtat tacatggcgc tggaatttgt
     3361 cagtggtaaa acgctagata gcattatcaa aaagctcggt aaggttccat ggcgagaagc
     3421 gctaaattac gcgattgctg cattggatgg actggaacac gcacatcagc taaaaatcat
     3481 tcaccgtgat attaagcccg caaatatcat gataaatgag caaggcaacg ttaaaatcat
     3541 ggatttcgga attgccagaa ttctgggaaa agagcgaatg actcagatag ggcgttttat
     3601 cggaacctta gaatatatgc cgcctgagca gattcaggga aaagaagctg atgcacgctc
     3661 tgatgtttat gctataggtg cagtcttata cgaaatgtta acagggcata ttcttttcga
     3721 acgtgatacg gaatttgaat tattaaattc acaaatctca gaaaagccaa aaccattacg
     3781 cgaattcaac catgaggtct cacctgaatt agaaaaaatc atattgcgtg ctttggagaa
     3841 agaacccggg aaacgttacg ccagtgcacg ggaattcagc gaagtaatac gtcgtttatt
     3901 acttgctgaa ccatcagcat ccttaactca tcaaaactct ctcattgcct tttgtaaaga
     3961 ttatccagct gtcgcaatcg ctgcctcact tttactacct gtcggtagtt acatagcatg
     4021 gcaattaaat tttgctgatc cttctcaggt ttcccaatcg gtggtatttc ctgtagagaa
     4081 gttagtggtt gagcctaccc aaagagcaat tgttgagaat caatctatcg aagctgaaag
     4141 aattacagcg attacatctg agaaaatacc tgagatgagg aattctttac aatcattccc
     4201 accaaattta ccctcgtccg ggaatgtaga tataggagac tcaaccgtgg aagatacatt
     4261 cgatatagaa tcagaattga tgcatgtctt gccaatgcct gaaaggaaac cggaggtgcc
     4321 tgagtatttt gttcaaacaa ataagcaatc tgctaagcaa atatcaacat cagcgcagca
     4381 gaatatcgta actaaaacga aaccttcttc ctctgctagt gataaaccag tcgatgatcc
     4441 tagaattgat gaatgggcta aagatttttt tgagaattaa catttacttt atgtttaata
     4501 aatctaatga agggaaatta ataatgaaac gattgataat agtaccactt gctgcaacgg
     4561 ctatcctcag tggttgtgct catacgacaa acctgagtaa ggaaggtcag gcaggtattg
     4621 gtgcgggttt aggtggtgcc cttggtgcgg caattggtta tgcagtatgt ggagcggagt
     4681 gtgcagcgat tggtgcggga gcaggtattg ttgctggtgg attgactgca tatgcgctag
     4741 ctagtgatcc ctatacacaa tcagcagcgc agcaggcaga gtcctggaaa aaggaaaccg
     4801 gtagcgaagc agaagttgtc aaagtatctg atgtaaccga gaatggtcaa aaacggcaac
     4861 gactggatgt gcagaaaatg caactatcag atgctcaggt agtcactaaa gatcgattgt
     4921 caccaagaat taaggaacaa ctaatcaaca caaagagttc agctcttaaa actggaggat
     4981 atgttcatgt agtctgtccc gcaaacgcac cttcttccgt aatgagcgat attgataaaa
     5041 ttgggattgg ctatagtaaa gacacaacca ctgccagtgg ttatactatt tatctttcta
     5101 gagacagggc tgagttaaag tcactaaata tataattagt tcattaatct attctttatg
     5161 gaggaaactt ttcttctcta cccattccgc tactttaccg taattttcat caactccttt
     5221 accatcactg tacatctcga taaccttatt tatcgctcca atatgatctt gatgtgccgc
     5281 agcttcaagc cagatgaaag cttctttata tttcttagaa tcaaataact gtttaccttt
     5341 ttggtagctt tgttcagccg acatatttaa tggaggtaat tcatcaattt gtgcgctcga
     5401 agaagtggta ggaggagggt taacaatatt taccaatggc ggcactataa cgaccaggga
     5461 aagaatgact gctgccacaa taaaaaactt tctccacggc ggaggcgggg gggcttcaat
     5521 aacaatgctt ggctgatgat taaattcttt aaatggtggt ccttcaggag aataccgtgg
     5581 atgacccaga agttcagtaa gccgtgtata tagttttggg aactctggat gatactgagt
     5641 tgctgatcgc cagtcgatta atttagcagt ttgattgcgc ctaaattcta gcggaatttc
     5701 aatattttct agcagaatag gaattagaat cttacgttta gcaccttctg aggcttcatt
     5761 tttcacccaa tctgaggcag ctgatgcttt ggtccacagc acaattacac attttgcagc
     5821 tttaagattt ttttcgatga cttcatcata agttaatcca ataggtattt gccgatccca
     5881 ccatactgtc cagccgagtt tttgtatcac aatagctaaa gtctcagcgt tagtacgatt
     5941 cgaactagca taacttagaa agatatcagc cattaatccc ctacctccac tgccataaat
     6001 attcgagata ggtatattct atcgaaagag tgttaggact agaagtgtat tttggagttt
     6061 gtagagaaca aataaactgt ccggggttgt agcacaagat ctgtccggat ttatatggaa
     6121 tccgaggagg atttcaaatg aagcggacag agtggctaca ggagacccgg aagatgagat
     6181 ttgaggaagc gtatgaaggc tatcgaagta agagtttgac gcaaagggag gctgcattat
     6241 tgctaggggt atgcgatcgg acatttcggc gttatatgga tcgatatgag gaaggtggtt
     6301 tgggtgcatt attggataag cgattgactc aagcatccca tcgctgcgcg ccgattgatg
     6361 aagttatgca attgacgcaa cagtatcgca aagactattc cggttggaat gccatgcatt
     6421 ttcatgcatg gtatcgtcgg gatggcggca cacgcagcta tacatgggtt aagaatcagc
     6481 tgcaagaagc agggttgatc aagcgtagca caaagcgtgg cgcacaccgg atgcgccgtg
     6541 aacgttcacc attacctgga atgatgattc atcaggatgg cagcacacat gaatgggtcg
     6601 ttaatcagaa atgggatttg attgaatcgc tccgggtttt ccggagacct tttagtttga
     6661 gtcaagccac attag
//
LOCUS       DAOQKQ010000149         6653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_5779_length_6653_cov_4.373750, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000149 DAOQKQ010000000
VERSION     DAOQKQ010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 6653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6653
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5779_length_6653_cov_4.373750"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..454
                     /locus_tag="PKZ49_09680"
     CDS             11..454
                     /locus_tag="PKZ49_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HND36873.1"
                     /translation="MGITRGVIKEVISLAGWFIAFYVASRYAESFEPLLPRVIEDPSL
                     RMLTLFVVVFFIVLLLVMFCSILLSKLIRSVGLGLIDRVLGSIFGLARGFAIVLSLVL
                     AAGFTALPKQPFWQQAVLSDPLEKAAVQVIPWLPESFRGHISFDK"
     gene            512..2032
                     /gene="purF"
                     /locus_tag="PKZ49_09685"
     CDS             512..2032
                     /gene="purF"
                     /locus_tag="PKZ49_09685"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HND36874.1"
                     /translation="MCGIIGVVAQSPANQILYDGLLMLQHRGQDAAGIVTAQDNTFYM
                     HKGLGMVRDVFRTRNMRALLGNMGIGHARYPTAGSADSPAEAQPFYVNSPFGIVLGHN
                     GNLTNAEELNKALYRSDRRHVNTNSDSEVLLNVLAHELQACARNDQLDPDDIFAAVAG
                     VHERCQGAYAVIAMIAGYGLLAFRDRLGIRPLVFGTAETEQGIEYMVASESVALDTLG
                     FKLIRDVAPGEAIFIDTKGNFHNKQCAANSSLNPCIFEYVYLARPDSMMDGFSVYETR
                     LNMGSSLAEKIKKTMRHLDIDVVIPIPESSRPSALQLANHLGIDFREGFVKNRYIGRT
                     FIMPGQQNRRKSVRQKLNAINVEFQGKNVLLVDDSIVRGTTSREIVQMAQEAGAHKVY
                     FASAAPPVRFPNVYGIDMPTRQELIAADKDEEQICYEIGADFLVYQELESLKNSISRI
                     NPAIKNFETSCFDGHYITEGITQDYLDKLEFQRSAGAKKLTKHDLTQLDLGLILAD"
     gene            2101..3264
                     /locus_tag="PKZ49_09690"
     CDS             2101..3264
                     /locus_tag="PKZ49_09690"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HND36875.1"
                     /translation="MDPETLALHTGIHRSQFNEHSEAMYLTSSYVFESAAQAAARFSG
                     EEPGNIYSRFTNPTVTAFQERLAVLEGAEECIATSTGMSAILACVLGLLSAGDHIVAS
                     RSIFGATVNLFNNILSRFNITTTFVSATETQEWADACKANTKLFFLETPSNPLTEISD
                     IAAIAQVAKNAGARLVVDNCFCTPILQKPLELGADIVIHSATKYLDGQGRVLGGAILG
                     KRDLLVDGGIYNFLRTAGPTLSAFNAWVLLKGLETLKIRIEAHSAHALEIANWLTEQP
                     NVARVFYPGLPSHPQHHLCKLQQKTGGGIVSFEVKGGREAAWRVIDNTSMISITANLG
                     DAKSTLTHPATTTHGRISQEARSAAGITEGLLRIAVGLESPKDIKADLAKGLG"
     gene            complement(3481..4080)
                     /locus_tag="PKZ49_09695"
     CDS             complement(3481..4080)
                     /locus_tag="PKZ49_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36876.1"
                     /translation="MKLSKFFALSFFISSFLLIVLSLQVSAAPNQPAMNVTGSVDANV
                     TNNVKVINPDGSALEVRIQPKKGSYTLSLNATATNDRGSFSTVIIPFDSYISGINFTT
                     GSKDSASCSTFVSYQGGGIPEGANTNEIAETGFASDGSRYAAPLYIMNSEYISIPNMF
                     IRGGDELLLTVTMNTHVFGATGNESCLGRAKIYLIPADK"
     gene            complement(4119..4850)
                     /locus_tag="PKZ49_09700"
     CDS             complement(4119..4850)
                     /locus_tag="PKZ49_09700"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HND36877.1"
                     /translation="MATQAELDAAVAAQATVDAGQNTTINTQQSTIETLQNEITSLNN
                     VNTAQQSALEKAQADIADLKVLIEKYHPEPKKIGDHFGGGIVFYIDPTGQHGLITSLF
                     DLSDKYIPWFNGINKITGATGDGIGVGAPNTALIVAAQTGDGGPDFYAAKLAADFSVQ
                     EDGVKPCTGEVGETCYRDWYLPSKFELNLLYQQKTVVGNFMPNEYWSSTEYDANNAWL
                     LNFTDGTQYFTDKYFTVRVRAVRAF"
     gene            complement(5089..6054)
                     /locus_tag="PKZ49_09705"
     CDS             complement(5089..6054)
                     /locus_tag="PKZ49_09705"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HND36878.1"
                     /translation="MALFRFSTQGFSESERVNAFQEIYASVANIDVEPYQQHPPFVEM
                     VGRLLPNVGVYETIISPHSSWRTRAHVAAENSDNLALVVPIDNTAIVIPKNKEPLHCL
                     PGDAVLIPSDSIHQALNTQTMSIAVIVLPTKLVATRNADYNERLMKKISSKDTPELPL
                     LVGYTRMLMQMKDDLSPELAALAATQIHDLFVLLCGAKQEEAAIAKDRGLRAARLRAV
                     KQDIVTHITDCELSINQVALRQGISPQYIRALFHSEETTFADYVTGLRLDQVYRFLRN
                     PLHLDRSISTLALDMGFNNISWFNRIFKQKFGLTPSEVRNSLINR"
     gene            complement(6289..>6653)
                     /locus_tag="PKZ49_09710"
     CDS             complement(6289..>6653)
                     /locus_tag="PKZ49_09710"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HND36879.1"
                     /translation="TAITNCTARTQSKLGIHSRAELVNFFAPNGFRRKLAETVLNGEH
                     LLIATNPLIKSHQLIGLTETEREIATYLIAGSTNADIARRRGSSEYTIANQVQSIFRK
                     LSVRSRGELIAKLQNEKI"
BASE COUNT         1867 a         1430 c         1415 g         1941 t
ORIGIN      
        1 ttctgttttt ttgggaatta cccgtggtgt catcaaggaa gtcatatcgc ttgcgggttg
       61 gtttatcgca ttttacgttg ccagtcgcta tgcggaatct tttgaacctt tactaccgcg
      121 cgtcatagag gatccctcat tacggatgct aacgcttttt gttgtggtct tctttattgt
      181 tttattatta gttatgtttt gctctatatt attatccaag ttaataagaa gtgtaggtct
      241 tggccttatt gacagagtgt taggatcgat tttcggttta gcgagaggat tcgcaattgt
      301 cttgtcttta gttttggccg ctgggtttac cgctttacca aagcagcctt tttggcaaca
      361 agctgtgttg agcgacccgc ttgagaaagc agccgttcaa gtcattcctt ggttacctga
      421 gagttttcga ggccacatta gctttgataa gtgaattatt ttatataccg ttattttcta
      481 aagcatcacg tttgaactca ttaaatcaat tatgtgtgga attattggcg tagtagccca
      541 atccccagca aaccaaatat tgtatgatgg tttgctcatg ttgcagcata gagggcaaga
      601 tgcggctggt atcgtaactg ctcaagacaa taccttctat atgcataaag ggctaggaat
      661 ggtacgagat gtattccgta cccgtaatat gcgtgcacta ctgggtaata tggggatagg
      721 ccatgcacgt tatccaacag cgggttccgc tgattctcct gccgaagcac aaccgttcta
      781 tgtaaactcg ccttttggaa tcgtgctagg acataatggc aatttaacca acgcagaaga
      841 actcaataaa gccttatatc gatctgatcg ccgtcatgtg aacacaaact cagattcaga
      901 agtattattg aacgttctgg cccatgaatt acaagcttgt gccaggaatg atcagcttga
      961 cccagatgat atctttgccg ccgtagccgg ggtacacgaa cgatgtcaag gtgcttatgc
     1021 agtaatcgcc atgatcgcag gatatggcct gctcgccttc cgcgatcgtt tagggatccg
     1081 gcccttggtt tttggaactg ctgaaactga acagggtatc gagtatatgg ttgcgtctga
     1141 aagtgtcgcg ctcgatacac ttggatttaa attaatccga gatgttgccc ctggtgaagc
     1201 gatcttcatc gataccaaag gtaatttcca taacaaacaa tgtgcagcaa actcaagtct
     1261 taatccttgt atttttgaat atgtttattt agcacggcct gattccatga tggatggttt
     1321 ctcggtttat gaaactcggc tcaatatggg cagcagcctt gcagaaaaaa tcaaaaaaac
     1381 gatgcggcat ctcgatatcg atgtggttat tccgatcccc gaatcgagtc gcccaagtgc
     1441 attacagctt gcaaatcacc taggaatcga ttttcgagaa ggatttgtca aaaatcgata
     1501 cattggtaga acctttatca tgccaggcca gcaaaatcga cgtaaatcgg ttcgtcagaa
     1561 attgaatgca attaacgttg agtttcaagg aaaaaatgta ttattggtcg atgactccat
     1621 cgtacgagga acgacaagca gagagatcgt gcaaatggct caagaagctg gagcacataa
     1681 ggtttacttt gcatctgcag cacctcctgt tcgctttccc aatgtttatg gaatcgatat
     1741 gccaacacgc caagaattaa tcgcggctga taaagatgaa gagcagattt gctatgaaat
     1801 cggtgctgac tttctcgttt accaagaact tgaatcgctc aagaattcca tttcacgcat
     1861 caatcctgct atcaagaatt ttgaaacttc ttgctttgat ggccactata tcacggaagg
     1921 gataactcaa gattatctcg ataaattaga atttcagcgc agtgccggag caaaaaagct
     1981 tactaagcat gatctgactc aactcgattt agggttaatt ctagcggact aaatcatata
     2041 atcctggaat tttttgcgct tatcatcatc tcgctattat cactatactt ttaacaaata
     2101 atggatcctg aaaccctagc acttcatacc ggcattcacc gcagtcagtt taatgagcac
     2161 tctgaggcca tgtatttgac ctccagctat gtcttcgaga gcgcagcgca agcagctgcc
     2221 cgcttctcag gtgaagagcc aggcaatatt tattcacgat tcactaatcc gacggtcacc
     2281 gcctttcaag aaagattagc cgttcttgaa ggtgcagaag aatgcattgc aacttccacg
     2341 ggtatgtcag caatccttgc ttgtgtgctt ggtttattgt ccgccgggga tcatatcgtg
     2401 gcctctcgta gcatttttgg tgcaacggtc aatcttttca acaatattct cagtcgattt
     2461 aatattacca ccacgtttgt gtctgcaacg gaaacccaag aatgggcaga tgcttgtaaa
     2521 gctaacacca aattattctt ccttgaaaca ccttccaatc ctttaacaga aatttctgat
     2581 attgctgcaa tcgctcaggt tgcaaaaaat gcaggtgcac gactcgttgt tgataattgc
     2641 ttttgtaccc ctattttgca aaaaccgcta gaactaggcg cagatattgt cattcactca
     2701 gcaaccaaat atttagacgg tcaagggagg gttttaggcg gggcaatttt aggaaaacgg
     2761 gatttgcttg tggatggggg tatttataac tttttacgaa ctgccgggcc caccttaagt
     2821 gcatttaacg cgtgggtttt gttaaaaggg ctagaaacac taaaaataag aatagaagct
     2881 cattcagctc atgcactaga aattgcaaac tggctaacag aacagcctaa tgttgcacgc
     2941 gtattttatc ctggcctacc ctctcacccg caacatcatc tgtgtaaact ccagcaaaaa
     3001 acgggtggtg gaatcgtctc cttcgaagtc aaaggcggta gagaagccgc atggcgcgtc
     3061 attgacaata ccagcatgat ttcaattact gcaaacttgg gtgatgctaa aagtacacta
     3121 actcaccccg ccacgacaac ccatggcaga ataagccaag aagccagaag cgctgcagga
     3181 atcacggagg gattactacg aatcgcagta ggactggaat cacccaaaga catcaaagct
     3241 gatcttgcta aagggttagg ctaataccaa caggagatta gcttaaagca caagtgtagt
     3301 atttgtctga tcaaccggac tgcagcagta caaaagtctc gggttatgtt tttatcgatg
     3361 caaaccagat ccttttattt gtaatgaaat gaactaacag caagctttcc gcagtatttc
     3421 taacccaccg tcaagaataa cttgtagtgg tgagttagaa aataaaaaaa taggctacat
     3481 tcacttatca gctggaatta aatagatttt tgcccttcct aggcaacttt catttcctgt
     3541 tgctccgaac acgtgtgtgt tcattgtaac agtcaacaac aactcatccc cgcctctgat
     3601 aaacatattg ggtattgata tgtattctga attcattata tacagaggag ctgcatatct
     3661 acttccatct gaagcaaaac cagtttctgc aatttcattg gtattagcac cttcaggaat
     3721 acccccaccc tggtaagaga caaacgttga acatgaagcc gaatctttac taccggtagt
     3781 aaaattaatg ccagaaatgt aggaatcaaa gggtattatc actgtactaa aacttcctcg
     3841 atcattcgta gcagttgcat ttaaggacaa agtatacgaa cctttcttgg gttgaatgcg
     3901 gacctccagt gcactaccgt caggattgat aactttcaca ttattcgtaa cattagcatc
     3961 tacacttccg gttacgttca ttgcaggttg atttggagct gcagaaactt gtaaggagag
     4021 gactatcagc aaaaagcttg atatgaaaaa agacaatgca aaaaatttag ataatttcat
     4081 attaacctcg tgtttttagt ttaagacagg attattcatt aaaaagcccg aacagcccgc
     4141 actctgactg tgaagtattt atcggtaaag tactgggttc catcggtgaa gttcaggagc
     4201 cacgcgttgt tagcgtcata ctccgtggaa ctccaatatt cgttaggcat aaaattgcct
     4261 acaacggttt tctgttggta gagcaggttc aattcaaatt tggaaggtag ataccagtcc
     4321 ctgtaacaag tttcacccac ttcgccagtg cagggcttca ccccatcctc ctgaacgcta
     4381 aaatctgcgg ccaattttgc tgcataaaag tcaggcccac catcaccggt ttgggctgcg
     4441 acgattaatg cagtattcgg cgcacctaca ccgataccat ctccggtagc ccccgtgatt
     4501 ttattaatgc cattgaacca tgggatatat ttatccgaca aatcaaacaa ggaagtaatc
     4561 aatccatgtt gtcctgttgg gtcgatatag aagacgatac cgccaccaaa atgatcccct
     4621 atcttcttgg gctcaggatg gtatttttca attaatacct tcagatcggc gatatctgct
     4681 tgtgcttttt ccagtgctga ttgttgtgct gtattgacat tattcagtga agtgatttca
     4741 ttttgcagtg tttcgatggt actttgctgg gtgttaattg tcgtattctg tccagcatcc
     4801 accgttgcct gcgcagcaac tgcggcatcc aactcggctt gcgtggccac atcggttttg
     4861 gcagcaaaag tatttgccag ttaatccaac agccatgact agcagagcag cagtcataat
     4921 tgttgaaaat ttgatattcc gatacctatt cattattact ccttattttt atggtattta
     4981 agaaacgatc ctgctctact gaccgtgcaa tcgttttacg ttaatttctc aaattgagct
     5041 actactgagc gctatactac ttgtactaaa cgctactttt gagatgattc agcggttaat
     5101 taaagaattg cgcacttccg agggagtcag tccaaatttt tgcttgaaga tacgattgaa
     5161 ccaggagata ttattgaacc ccatatccaa agctaatgtg ctgatgcttc tatcaaggtg
     5221 taggggattg cgcagaaaac gataaacctg atcgagacgt aatccggtaa catagtctgc
     5281 gaatgtggtt tcctcgctgt gaaacaaagc gcgaatatat tgaggggata tgccttgacg
     5341 cagagcaact tgattgatgg acagctcaca atcggtaata tgcgtcacga tatcttgctt
     5401 taccgctcta agccgtgctg cgcgcaaacc acgatccttg gcaattgccg cctcttcctg
     5461 ttttgcgccg cacagcagca caaataaatc atgaatctga gtagccgcca atgccgcaag
     5521 ttcgggcgac aagtcatctt tcatttgcat cagcattctg gtgtaaccca caagcaaggg
     5581 taattccggc gtatccttgc tagagatttt tttcatcaaa cgctcgttat agtcagcatt
     5641 tctggttgca accaactttg tcggtaaaac gattacagca atggacatcg tttgggtgtt
     5701 aagagcctga tgaattgaat ccgaaggtat cagtacagca tcaccaggca aacagtgaag
     5761 cggctctttg tttttcggaa taacaattgc cgtgttatcg atgggtacaa ccaatgccag
     5821 attgtcactg ttttcagcag cgacatgagc gcgtgtacgc catgatgaat ggggtgagat
     5881 gatggtttcg tatacaccca cattcggaag caacctacca accatctcaa caaaaggcgg
     5941 gtgttgttgg tatggttcaa catctatatt ggcaacagat gcataaattt cttggaatgc
     6001 gttaacacgc tcgctttcgg aaaaaccctg agtagaaaat ctaaatagcg ccattttttc
     6061 agatttacca gatttattta taactctgta tttcacatga aattttccat gtagaaacat
     6121 cgcttatata aaaataactg tatacttcaa tacaagccaa tcctacaatt atggtaaatt
     6181 ctcatcaata gttaattcca cacattactt ttgatacaag ttgatcttac aattatggtt
     6241 aattgctcat agtaatattg acgatgcggc tgatattgaa ttgtgcggtt agattttttc
     6301 gttttgaagc tttgcaatca attccccgcg cgaacgcacg gataatttac ggaagatcga
     6361 ttgcacttgg ttggcaatgg tatattcact cgaaccgcga cgccgtgcga tatctgcgtt
     6421 ggttgatcca gcaatcaggt aagtggcaat ttctcgctct gtctcggtaa gcccaataag
     6481 ctggtgagat ttaatgagcg gattggtagc aattagcaaa tgctctccat ttagcacagt
     6541 ttctgccagc ttgcgccgga atccattcgg tgcgaagaag ttcactaatt cagcacggga
     6601 atgaattccc agtttacttt gcgtacgtgc ggtgcaattg gtaatggcgg ttt
//
LOCUS       DAOQKQ010000150         6606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_5838_length_6606_cov_6.367120, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000150 DAOQKQ010000000
VERSION     DAOQKQ010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 6606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6606
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5838_length_6606_cov_6.367120"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PKZ49_09715"
     CDS             complement(<1..248)
                     /locus_tag="PKZ49_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36880.1"
                     /translation="MIQRCNNVLIRLIGMVFALALVLSISNVLATNQFNQPKPVVVAD
                     SSAIPAITPNPSNTVKFETITVETSAASELTAEPIQKL"
     gene            complement(245..1129)
                     /locus_tag="PKZ49_09720"
     CDS             complement(245..1129)
                     /locus_tag="PKZ49_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36881.1"
                     /translation="MIKKFNCAVLVILLISFGINAVAHEGEPNMGFAWREGVIEIDIR
                     KQGTKLGDLSAFVIRFTDSIAPYRMGDAGFTGIEFDQGGILGYQAETHLMKWSSEQSQ
                     WLTEGFSEQIIISRLSDEKRISASEGKGIQGFIENLTNTSTFESHPIFKIQKPDGQVP
                     EDGAYMVIITVLGVDATGENILYQPSQPFALVFHINAKGNFNGLALSEALDVSPVLKL
                     NNYSRIDALFNWAEENYSRYFPHAVKSRFIEGYYARCYNNGTCLGSLDGKIYTLGGEL
                     GGLTDHGAIETFYQAAGL"
     gene            1286..2269
                     /locus_tag="PKZ49_09725"
     CDS             1286..2269
                     /locus_tag="PKZ49_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36882.1"
                     /translation="MSPYRLIFAAVISLLISLPSFAGELHQHIEVNTTNQRVQVNFCR
                     PERGCSLVKPETIGLPPGQLPLDKLTGTAIYVSEFTALPNGIAIDSPGFEGIAGDLNA
                     GDRVHYVASSRLSFWNPAERQWSLAPEGIRIRLAGGLDLQPDQSCGQVFCLPKAVEGF
                     TIFNRQGVSGINSLIVGEARPDGSLHTHLDWILETEQGAPNGPIGAYLVELSIRTDAY
                     PTPSAPFWIMFNHSLPITEFQQAVADRVLKNSIDTALADKLFDWAESNYLSLFPNKTT
                     SFIAVGYYARCYQNGTCVGVRDNYVFAVGGEFGPNVVNLGEFSSLLKLAGF"
     gene            complement(2429..3544)
                     /gene="dnaJ"
                     /locus_tag="PKZ49_09730"
     CDS             complement(2429..3544)
                     /gene="dnaJ"
                     /locus_tag="PKZ49_09730"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HND36883.1"
                     /translation="MAKRDYYEVLGISRDADENEIKKAYRKLAMKHHPDRNAGDTKAE
                     EKFKEAKEAYEILSDASKRAAYDQYGHAGVDTSAAGAGHGQGFGDAFSDIFGDIFGMR
                     GGGGRSNVHRGADLRYNLEITLEQAARGSETKIRIPTMTTCDTCNGSGAKPGTSPKTC
                     STCDGHGQVRMQQGFFSIQQTCPKCHGSGKMITSPCSSCHGAGRIKQHKTLNVKIPIG
                     VDDGDRIRLSGEGEAGINGGPAGDLYVVIQLSSHPVFQREGNDLHCEIPISFATAALG
                     GEIEVPTLEGHAKIKISAETQTGKIFRLRGKGIKGVRSNSAGDLLCHVAVETPVNLTA
                     RQKELLQELEDISQKDNSRHSPRAKSWMEKAREFFSE"
     gene            complement(3740..5671)
                     /gene="dnaK"
                     /locus_tag="PKZ49_09735"
     CDS             complement(3740..5671)
                     /gene="dnaK"
                     /locus_tag="PKZ49_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HND36884.1"
                     /translation="MAKIIGIDLGTTNSCVAVMENGKPKVIENSEGTRTTPSIVAYTD
                     DNEILVGASAKRQAVTNPKNTLFAVKRLIGRRFDEDMVQRDIKMVPYSIVRADNNDAW
                     VEVRGRKIAPPEISAQVLIKMKKTAEDYLGEPVSEAVITVPAYFNDSQRQATKDAGRI
                     AGLEVKRIINEPTAAALAFGMDKKEGDRKIAVYDLGGGTFDISIIEIAEIEGEHQFEV
                     LATNGDTFLGGEDFDSRVIEYLVDEFKKESGIDLKKDMLALQRLKDAAEKAKIELSSS
                     QQTEVNLPYITADASGPKHLAIKITRAKLESLVEELIERTIEPCRIAIKDAGLSFSDI
                     HDVILVGGQTRMPKVQEKVKEIFGKEPRKDVNPDEAVAIGAAIQGGVLQGDVKDVLLL
                     DVTPLSLGIETLGGVMTKLIQKNTTIPTKANQVFSTADDSQTAVTIHVLQGEREMASG
                     NKSLGQFNLTDIPPAPRGMPQIEVTFDIDANGILHVSAKDKATGKENKIKIQASSGLS
                     EDEIQRMVKDAETHAEEDKKALELVNSRNQCDAMIHSVRKSLGEYGDKLEADEKAKIE
                     AALKEAEEALKSNDKDIIDAKTQALTQASQKLAEKIYAQDQTQSEQPKTESTAQQGGE
                     KTVEGEVVDAEFEEVKTKK"
     gene            complement(5746..6327)
                     /gene="grpE"
                     /locus_tag="PKZ49_09740"
     CDS             complement(5746..6327)
                     /gene="grpE"
                     /locus_tag="PKZ49_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HND36885.1"
                     /translation="MQESHNSSSSEQELISVGTETSSASNTGNTVSDSVIESTPSLEQ
                     LLRDAELKAAEHHDAWLRAKADAENIRKRAQADIASAHKYAIEKFSVELLAVMDSLDA
                     ALAVENVTLENFRNGVELTHKQLTNVFEKFNIKAISPVGEKFDPHQHQAMCMVESELP
                     ANTVTQVMQRGYLLFDRVIRPAMVAVSKNKEGT"
     gene            complement(6407..>6606)
                     /locus_tag="PKZ49_09745"
     CDS             complement(6407..>6606)
                     /locus_tag="PKZ49_09745"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112483.1"
                     /note="Catalyzes two discrete reactions in the de novo
                     synthesis of purines: the cleavage of adenylosuccinate and
                     succinylaminoimidazole carboxamide ribotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HND36886.1"
                     /translation="FGVKNPYERLKALTRGKSGISQEALHQFIQTLEIPDKEKQQLLA
                     LTPTNYIGLAEKLAKDFHCRN"
BASE COUNT         1749 a         1528 c         1247 g         2082 t
ORIGIN      
        1 tttaacttct gaataggctc agcagttaac tctgatgcag ctgatgtttc aaccgtaatc
       61 gtttcaaatt taactgtatt gctgggattg ggtgtaattg caggaattgc agatgaatca
      121 gcaacaacaa caggtttcgg ttggttaaat tgattggttg ctaggacatt acttatcgat
      181 aaaactaaag ccaaagcgaa aaccatgccg atcaatctga tcagcacatt gttgcaccgc
      241 tgtatcataa acctgctgct tgatagaaag tctcaattgc accgtgatca gtaaggccgc
      301 ccaactcacc accgagcgta taaatttttc catctaaact gccaaggcaa gtaccattgt
      361 tgtaacaccg tgcataatag ccttcgataa aacgactttt tactgcgtgt gggaagtaac
      421 gactgtaatt ctcctcagcc caattaaaaa gtgcatctat ccgactataa ttgtttaact
      481 tgagcactgg agagacatcc aaagcttcgc tcaatgctaa accattaaaa ttcccttttg
      541 cgttgatatg aaaaactaat gcgaacggct gggagggttg ataaagaata ttttctcccg
      601 tcgcatcaac accgagaact gtaatgatca ccatataggc accatcctcc ggtacttgac
      661 catctggttt ctgaattttg aaaatgggat gagattcgaa tgtgctggta ttggttaaat
      721 tttcaataaa tccttgaatc cctttacctt ctgaagccga tattcgcttt tcatctgata
      781 atcgactaat gataatttgt tctgagaatc cttcggtcaa ccattgcgat tgctcacttg
      841 accatttcat caaatgcgtt tctgcttgat acccaagaat ccccccttga tcaaattcta
      901 tgccggtgaa tcctgcatca cccatacgat aaggagcgat ggaatcagta aatcgaatta
      961 caaaagcgct aagatcaccc aatttagtgc cttgttttct gatatcaatt tcaataaccc
     1021 cctctcgcca ggcaaagccc atattgggtt caccttcatg agcaaccgca ttgataccaa
     1081 agctgattag taagattacc aaaacagcac aattaaattt tttgatcatt attcttaatt
     1141 aaactcactc gatttattgc gggtgatact agctcagggg aagttgaaag gcaatgcgac
     1201 aaattgtcgc attgccccga tagggaatat ttgttagatt ggtacgcgtc tataccctgt
     1261 caaccatcat ttcagttatt tcttcatgtc accttatcgc ttgattttcg ctgctgtaat
     1321 aagcttactc atatcgctcc cctcatttgc tggagagctg caccaacata ttgaagtcaa
     1381 tacaacgaat caacgtgtgc aagtcaattt ttgtcgccct gaaagaggct gtagtttggt
     1441 aaaaccagaa acgattggat taccgccagg ccagctcccg cttgataagc taacgggtac
     1501 agcaatttac gtttctgaat ttacggcgct tcctaacgga attgcaattg atagccctgg
     1561 atttgaaggt attgcgggtg atttgaatgc aggtgaccgt gtccattacg tggcgagtag
     1621 ccgacttagt ttctggaatc ctgctgaacg tcagtggtct ttagccccag aaggaatccg
     1681 tattcgttta gcgggtggtt tagatttaca gcccgatcaa tcctgtggtc aagtgttttg
     1741 tcttccaaaa gctgtcgagg gatttacgat atttaatcga caaggtgttt caggcatcaa
     1801 ctcgttaatc gtgggtgaag ctcgtcctga tggctcatta cacacccatc tggattggat
     1861 tcttgaaaca gaacaaggtg ctccgaatgg accgataggc gcttatttag ttgagttaag
     1921 tataaggact gatgcttatc ccactccttc tgcacctttc tggatcatgt ttaatcatag
     1981 tctaccaatc actgaatttc agcaagcggt tgcagaccga gttttgaaaa acagcattga
     2041 tactgctttg gctgataagc tttttgactg ggctgaatca aattacctat cgctatttcc
     2101 gaataaaact acttctttta ttgcagtagg gtattatgcg cgctgctatc aaaacggaac
     2161 ttgtgtcggg gttagagata attatgtttt tgctgttggt ggagaattcg gtcccaatgt
     2221 agtcaatctt ggagaatttt cttcattatt aaagttggca ggattttagt ttaggtctga
     2281 agaaaaagtt tttcgatttt tcatacttat aaaaattaat gaatctggcg aggctggatg
     2341 tgtaattaaa acatctcttg gagatgtgac atgaacaata tcgtgaactc agccttgcca
     2401 gattcattaa cccctatgca ggcgaaggtt actctgagaa aaattcacgc gccttttcca
     2461 tccatgactt cgctcgagga ctatgcctag aattatcttt ctgactaata tcctctaact
     2521 cttgcaacaa ttctttttgg cgcgcggtca aattgaccgg cgtttcaact gcaacatgac
     2581 ataacaaatc gccagcagaa ttactgcgta ccccttttat acctttacct cgcaaacgaa
     2641 atatcttacc ggtttgagtt tcagcggata tcttgatttt tgcatgtccc tccaaagtag
     2701 ggacttcaat ctctccacct aaggcagccg ttgcaaaact gatgggtatt tcacagtgca
     2761 agtcgtttcc ttctcgctga aatacgggat gacttgatag ttgaattaca acatacaaat
     2821 cgccagccgg gcctccgttg attccggctt caccctctcc agataatcga attcgatctc
     2881 catcatcaac ccctatcggt attttgacat ttagcgtttt atgctgctta atacgccctg
     2941 cgccatggca agaagagcaa ggcgaagtga tcattttgcc gctaccatga catttcgggc
     3001 aagtttgttg aatcgaaaag aaaccttgct gcattctgac ctggccatgc ccatcacagg
     3061 ttgagcaggt ttttggcgat gttccaggct tcgcaccaga accattacag gtatcgcaag
     3121 tcgtcatcgt cggaatacga atttttgttt cggaaccccg cgctgcctgc tcaagcgtaa
     3181 tttctagatt ataacgaagg tctgcaccac gatggacatt ggagcgccct cctcctccgc
     3241 gcattccaaa aatatcacca aaaatatcgc tgaacgcgtc accaaaaccc tgtccatggc
     3301 cagcaccagc agcactggta tccacacctg catgtccata ttggtcataa gctgcacgct
     3361 tactggcatc agacagtatt tcataggcct ctttggcttc tttaaatttt tcttcagcct
     3421 tcgtatcgcc tgcatttcga tcaggatggt gtttcattgc caatttacga taggctttct
     3481 tgatttcatt ctcatcggca tccctgctaa tgcctagaac ttcataatag tctcgcttag
     3541 ccatataagt ttcctgttac atgggtacaa tcaatatcaa ttagtactgc cattgattat
     3601 ttcccaatgg gtataacgta attatcttga gcattaatga aataatcatt gctcatctca
     3661 aaacaaaacg cagagtatgc aagaaaaaga attgataatt tcttgcatac tctgcgttag
     3721 tcccaaaata atttacaatt tatttcttcg ttttaacttc ctcaaattct gcatcgacta
     3781 cctcaccttc taccgttttt tcgccccctt gctgagcggt tgattcggtt tttggctgct
     3841 cactttgtgt ttgatcctga gcgtagattt tctcggcaag cttttgtgac gcttgagtca
     3901 aagcttgggt cttagcatca atgatatctt tatcatttga tttcagcgct tcctcagctt
     3961 ctttcagtgc tgcctcgatc ttagcctttt catcagcttc aagcttatca ccatattccc
     4021 ctagtgattt tctaactgaa tgaatcattg catcacattg gttcctgcta ttaaccaatt
     4081 ctagtgcctt tttatcttcc tctgcatgcg tttcagcatc tttgaccata cgctgaattt
     4141 catcttccga taaacctgaa cttgcttgaa tcttaatttt gttttcttta cccgttgctt
     4201 tatccttggc cgatacatgc aaaataccat ttgcatcaat atcaaacgtt acttcgattt
     4261 gaggcatgcc acgcggagcg ggaggaatat cggtcaagtt aaattgaccg agacttttat
     4321 ttccggaagc catttcacgt tcaccttgca gcacatgaat tgttacagca gtttgactat
     4381 catcggcggt tgaaaatact tgatttgctt ttgttggaat agtggtgttt ttttgaatca
     4441 actttgtcat gacaccgccc aatgtttcga tacccaacga taatggtgtc acatccaaca
     4501 acagcacatc ttttacatcg ccttgcaaca ccccaccttg aatcgcagcg ccgatcgcaa
     4561 ctgcttcgtc aggatttaca tctttacgag gctctttacc aaaaatctcc ttaactttct
     4621 cctgtacttt aggcattctt gtttgtccgc ccaccaagat gacatcatgg atatccgaga
     4681 aagataaacc cgcatctttg attgcaattc gacatggctc aatggtgcgc tcaatcagct
     4741 cttcaaccaa actttctaat ttagcgcgtg tgattttaat cgcaagatgc ttaggaccac
     4801 tggcatctgc cgtaatgtag ggcaaattca cctctgtttg ctggcttgac gatagttcga
     4861 ttttagcttt ttcagctgca tctttgagtc gttgtaacgc tagcatatct ttcttgagat
     4921 cgatgccact ttctttctta aactcgtcaa ctagatattc aataactcga gagtcaaaat
     4981 cttcaccacc gagaaatgta tcaccgttgg tggcaagcac ctcgaattga tgctcacctt
     5041 caatctcagc aatttcaatg atagaaatat cgaatgttcc gccaccgaga tcataaaccg
     5101 caattttccg gtcaccttct tttttatcca tcccaaatgc caatgcagca gcagtgggtt
     5161 cattgataat gcgctttact tccaagccag caatacgccc cgcatctttc gttgcctgcc
     5221 gttgtgaatc attaaaataa gcaggaactg tgatcacagc ctcagacaca ggttcaccca
     5281 agtagtcttc agcagttttc ttcattttaa tcaaaacttg tgctgagatt tctgggggtg
     5341 ctattttcct tccacgtact tccacccacg catcattatt atctgcacga acgatactat
     5401 agggcaccat cttgatatcg cgttgtacca tgtcttcatc aaagcgacga ccaatcaatc
     5461 gcttaactgc gaacaaggtg tttttaggat tggtaacagc ttgacgtttc gccgaagccc
     5521 cgacaagaat ctcgttatca tcggtataag cgacaatcga tggtgtagtc cgcgtacctt
     5581 ccgagttttc gattacctta ggtttaccat tctccattac tgccacacag gaatttgttg
     5641 ttcctaaatc gattcctatt atttttgcca ttctggttta gtcctttaat aaataatatc
     5701 aattaccgct aattagggtc tgaaatgggg attcatcgag aaaattcaag taccttcttt
     5761 attttttgaa acagccacca tggcgggtcg aatcactcga tcaaacaaca gatatccgcg
     5821 ttgcataacc tgggtaacag tattcgctgg aagttccgac tccaccatac acatcgcttg
     5881 atgctgatga ggatcaaatt tctctccgac tggacttatt gctttaatat tgaacttttc
     5941 aaaaacattg gtaagctgct tgtgcgttaa ctcaacacca tttctaaaat tttctaatgt
     6001 gacattttcc acagccagtg ctgcatcaag actatccata accgcaagta attcaactga
     6061 aaatttttcg attgcatatt tgtgtgcgct tgcaatatcc gcttgagcac gtttacgaat
     6121 attctcggca tcggctttgg cacgtagcca agcatcatgg tgctcggctg ctttcagctc
     6181 agcatcccta agtaattgtt ctaaactcgg cgttgactca ataaccgaat cagaaacagt
     6241 atttcctgtg ttactagcgc tactcgtttc tgtaccaacc gaaataagtt cttgctcgct
     6301 tgatgatgaa ttgtgcgatt cttgcatata tcctctctgt aatttttcag gttatgtggg
     6361 gcgcattttg ctaatttcaa gttcttttct gaaaaattta tcaaccctaa tttctacaat
     6421 ggaaatcttt cgctaatttt tcagctaaac caatataatt tgtaggcgtc aacgctagga
     6481 gttgctgctt ctccttatca ggaatttcca gcgtttggat aaattgatgt aaggcttcct
     6541 gagaaatgcc agattttcca cgcgtcaatg ctttcaatcg ttcataggga ttttttaccc
     6601 caaaac
//
LOCUS       DAOQKQ010000151         6597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_5857_length_6597_cov_4.887343, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000151 DAOQKQ010000000
VERSION     DAOQKQ010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 6597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6597
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5857_length_6597_cov_4.887343"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /gene="purB"
                     /locus_tag="PKZ49_09750"
     CDS             complement(<1..931)
                     /gene="purB"
                     /locus_tag="PKZ49_09750"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HND36887.1"
                     /translation="MTFQTICALSPLDGRYHAKTSALRPYFSEFALIRYRVIVEIEWL
                     KALSNESLIGEVSPFSANTVGKLENLIKNFSVTDAEAIKAIEASTNHDVKAVEYWLKE
                     TFSQYSDIANVIEFIHFACTSEDINNLSHGLMLKNSRDEVMLPMLHALIDKLQTMAHQ
                     FASVPLLSRTHGQPATPTTMGKEMANVAYRLNYACDRLRAIALLGKINGAVGNYNAHI
                     VAYPNLDWEKFAQNFVEKLGLTFNPYTTQIEPHDAIAALFDAYARVNTVLIDLNRDIW
                     GYISLGYFKQKTKENEVGSSTMPHKVNPIDFENS"
     gene            1248..2408
                     /gene="hpnH"
                     /locus_tag="PKZ49_09755"
     CDS             1248..2408
                     /gene="hpnH"
                     /locus_tag="PKZ49_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HND36888.1"
                     /translation="MGIPFLQKYRVGSYILKQKILGVKRYPLVLMLEPLFQCNLACAG
                     CGKIDYPDETLKRRLSVQECLDAVDECGVPVVSIAGGEPLIHKEMPQIVAGIIQRKKF
                     VYLCTNALLLDDRMDDYKPSPYFTFSIHLDGNKERHDTSVCRDGVYDKVIPVIKKALE
                     RGFRVTVNCTLFQNETAEEVADFFDTATELGVEGINVSPGFSYEHAPRQDVFLQRSVS
                     KRLFRAIFQEGKKRKRAWKFNHSSLYLDFLAGNQSYQCTAWGNPTRNLFGWQRPCYLL
                     VDEGYASSFRELMEDTNWDKYGVGKNPKCANCMAHCGFEPTAINDTFAHPIKAMRVSL
                     NGPKTDGPMAIDPSQQGSTTGASNAQKDFPIPVVVEPKAVKASSSGDVSNKK"
     gene            2487..3611
                     /locus_tag="PKZ49_09760"
     CDS             2487..3611
                     /locus_tag="PKZ49_09760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HND36889.1"
                     /translation="MFEIFTVGFACFGALLWWIIVLLPWKPTSTKEYLESNPRLNSEQ
                     ISNVTVLIPARNESASIERTLQALSTQGKNLPIIIVDDQSEDDTSEKAKNFPVTIISG
                     TTLPTGWSGKLWALEQGLAHIKTSYVLLLDADIELDNGMVATLLQKAEHEQLAFVSLM
                     AEPNMDSFIERLLMPAFVFFFKLLYPFKLSNTPQSKIAAAAGGCILVKREALHQIGAF
                     NCLRNALIDDCTLANRVKQAGFLTWTGLSHSVKSHREYQTLMPIWDMVARTAYTQLNY
                     SIFWLLVCTLLMISLFWFAPVGSFLAEEWLGVMIGLFTWLAMLVAYIPTLQFYRRSLL
                     WVVALPFIGFLFLLMTWTSALKYWRGERALWKNRRYEISL"
     gene            3595..4230
                     /locus_tag="PKZ49_09765"
     CDS             3595..4230
                     /locus_tag="PKZ49_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpnM family protein"
                     /protein_id="HND36890.1"
                     /translation="MKSRYNFILLRYLIIFSVVLFVPALSHSQPTESETPEQVINELQ
                     ATLIQVMQKGTELGYQERFNLLAPVVQRSHDLPAIIKAVLGTHWNKLTEEQHQAITET
                     FSKLSIATYAERFNQHDQERFEFIEKRTLPKEQVLVRSQLIQADGKAVNFDYVMRQGA
                     SHHWKIINILADGVSDLALKRAEYSAIIQRDGFSALLSLLEQKIIQAEQHH"
     gene            4349..>6597
                     /locus_tag="PKZ49_09770"
     CDS             4349..>6597
                     /locus_tag="PKZ49_09770"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HND36891.1"
                     /translation="MKPTDQQERVLIAWAAFCYRYAFWVLCFLLITGIACGTYTARNL
                     EVLTDTADMLSEELPFRVNLAKLNKAFPQYENTLILVLDAPTPEQAHAAASRLTSYLK
                     DGHIEAYDIYYGAGEPFLEQNGLLYKNIAELEFLADRLAAAQPLIVRIAQNPSLLTFI
                     QTLTEAIDAFRSGNNLELDPVLDGISATLTARLAHSPRPLSWQALFNDEHPQSSYKEI
                     IILQPKLDYTELFAAKDSIDAIRAAFQTLNLENDFLTKLRITGEVALSYEELLSAMHG
                     AEYAGLVALIIVTLILFVALRSAGAIITVLVSLLLGLVITAAFTTATIGHLNLISIAF
                     AALYIGLAVDYAIHLILRYHELNRPDQPVIEILLSAIHDIGPSLLICALTTAIGFYAF
                     IPTTFLGIAELGIISGTGMFISLFITLTVVPALQRFLPSKIYSQVRKNRSITQQWLAL
                     PQKWPKTVYVLVALSFALALTTWNQIRFDYNLLNLSDPNGEAVLTFRELLADTQHSPW
                     LSMATVKSLEEANNLATQLTNLPVVDKVISIADFIPDQQDEKLQIIEGIALTLGPSFF
                     TENHVENDDLESSDFTQINQALENLRYQLNQLMIEKPNHLSIESWKRLSFSIDQLLTF
                     IHTVPAGQVQPFLNALQNDLIGQLPGVLNRLQLALEASTIKDESLPSSLRQRWHNESD
                     DFLVAVYPSENLNDNDALKRFIRSVQQHAPQISGTPAIMLAAGEAVVDAFIHAFILTL
                     LGVTLSLYV"
BASE COUNT         1921 a         1432 c         1298 g         1946 t
ORIGIN      
        1 cagaattctc aaaatcaatc ggatttactt tatgcggcat tgtcgaggaa ccaacttcat
       61 tttccttggt tttctgcttg aagtagccta atgaaatata gccccaaata tctcgattta
      121 aatcgatcaa aactgtgttc acgcgtgcat aggcatcaaa taatgctgca attgcatcat
      181 ggggctctat ctgagtcgtg tagggattaa atgtcagccc tagcttttcc acaaaatttt
      241 gagcaaactt ttcccagtcc aagtttggat aagcaacgat atgtgcatta tagttcccca
      301 cagcgccatt gattttcccc aatagagcaa tcgcccttag ccgatcacag gcataattca
      361 aacgataagc gacattagcc atttccttac ccatcgttgt tggcgtagca ggctgtccgt
      421 gtgtacgact caataatgga acactggcaa attgatgtgc catggtttgg agtttatcga
      481 ttagcgcatg cagcataggt agcatcactt catcacggct atttttgagc atcaaaccat
      541 gggagaggtt gttaatatct tcagaagtac acgcaaagtg aataaattca atcacatttg
      601 caatatctga gtactgagaa aaagtttctt ttagccaata ttcaaccgct tttacatcat
      661 gattagtgct tgcctcaatt gctttgatcg cttccgcatc ggttactgaa aaatttttta
      721 ttaaattttc taatttacca acggtgttcg ctgagaacgg agaaacttca ccaatgagtg
      781 attcgttact caaagcctta agccattcaa tttcgacgat aactcgatag cgaattaagg
      841 caaattcact aaaatagggc cttaaagcag aagttttagc atggtagcga ccatccagcg
      901 gggaaagtgc gcaaatggtt tgaaaagtca tgaaatatat cctgtttttc ctgctatttt
      961 atcttaatta cacgataaga acgcggcaca aattaaagaa attaattcca tgactacagg
     1021 attgatgaat cgaactgatt cactcatgtg atgatattta ttgcagttgt cagtctattt
     1081 ttttataact tatccaataa aaatcgcttt gaccttaata gtgtctcatt tttgtttacg
     1141 ccaaatccac ataactcaat gttaattccg ctaaaacttc agtttattag cgagccatgc
     1201 taggattacg aataatctta ccatcaaaaa tagagagagg aatttgaatg ggtatccctt
     1261 ttttacaaaa ataccgagtg ggaagctaca tattaaaaca gaaaatttta ggtgttaagc
     1321 gctatccgct tgtattaatg cttgaacctt tatttcaatg caatcttgct tgtgctggct
     1381 gtggaaaaat cgactatcct gatgagacat taaaacgtcg tctaagtgtt caagaatgtt
     1441 tggatgctgt tgatgaatgc ggtgtaccag tcgtttcgat cgcaggtgga gaaccgctga
     1501 ttcacaaaga aatgccgcaa attgtagcgg gaataataca gcgcaagaaa tttgtgtact
     1561 tatgtactaa cgcgctactt ctggatgatc ggatggatga ttataagcct tccccctact
     1621 tcacattctc tattcacctg gatggtaaca aagaacgcca tgatacctct gtatgtcgtg
     1681 atggggtata cgacaaagtg attcccgtta tcaagaaagc gcttgagcgg ggatttagag
     1741 tcactgtcaa ctgcacgctg tttcagaatg agacagcgga agaagtcgcc gatttttttg
     1801 ataccgcgac tgagttaggg gttgaaggta ttaacgtttc cccaggattt agttacgagc
     1861 atgctccacg gcaagatgtt ttccttcaac gttctgtcag taagcgttta tttcgtgcca
     1921 tttttcaaga aggtaaaaaa cgcaaacgcg cttggaaatt caatcactca agtttgtatt
     1981 tagattttct ggcaggaaat caaagctatc aatgtactgc gtggggcaat ccaacccgta
     2041 atttgttcgg atggcaacgc ccttgttatc tgctcgttga tgaagggtat gcctcaagct
     2101 tccgtgagtt gatggaagat actaattggg ataaatacgg cgtaggtaaa aatcctaaat
     2161 gtgcaaactg catggctcac tgcggctttg agccaactgc tatcaatgat acttttgcac
     2221 atccaatcaa agcaatgcgt gtcagcctaa atgggcctaa aactgatggt ccaatggcaa
     2281 tcgatccatc acaacaaggg agtactactg gcgcgagtaa tgcacagaag gattttccta
     2341 tccctgtggt tgtagagcca aaagcagtaa aagcttcttc ttcaggagat gtatcaaata
     2401 aaaaataaat aaccggtgga atttttattt cactacattt caaaacgtga aactagaact
     2461 ctaccggata cattgttatt cagattatgt ttgaaatttt taccgttggt tttgcttgct
     2521 tcggtgcgct tctttggtgg atcatcgtac tactgccttg gaagccaacg agcacaaaag
     2581 aatatcttga gtctaaccct cgattaaatt ctgaacaaat cagtaatgta acagttctca
     2641 taccagcgcg aaatgaaagt gcttcaattg aaagaacact acaagcgctg tcaacgcaag
     2701 ggaaaaattt accaatcatc atcgtcgatg atcaatctga agatgatacc agtgagaaag
     2761 ctaaaaactt tccagtaacc attatctcag gtacaacgct accaacggga tggagtggta
     2821 agctatgggc gctagaacaa ggattggccc atataaaaac atcttatgtg ttactgcttg
     2881 acgctgatat cgaactcgat aacggtatgg ttgcaacatt acttcagaaa gcagagcatg
     2941 agcagttagc gtttgtatcg ttaatggcag aacctaatat ggacagcttc atcgaacgtt
     3001 tgctgatgcc tgctttcgtt tttttcttta aactgctgta cccttttaag ctttccaata
     3061 ccccccaatc aaagatcgct gctgctgcag gtggttgcat tcttgtcaaa cgcgaagcgc
     3121 tacatcaaat cggcgccttt aattgccttc gaaatgcgct cattgatgac tgcacactag
     3181 cgaatcgcgt taaacaagcg ggttttctta cctggactgg attaagtcac tcagtcaaga
     3241 gccatcgaga gtatcagaca ttaatgccaa tttgggatat ggtggcacga acggcctaca
     3301 cacagttaaa ttattctatc ttttggcttc tggtgtgcac acttcttatg attagcctgt
     3361 tctggtttgc tccagttgga tcattcttag cggaagaatg gcttggcgtt atgattggtc
     3421 tatttacttg gttagccatg ttagttgctt atatacctac acttcagttc tatcgccgtt
     3481 cattgctatg ggtcgttgct ttaccgttta ttggtttttt attcctatta atgacctgga
     3541 catctgcatt gaaatattgg cgtggagaac gtgctttatg gaaaaatcgt cgctatgaaa
     3601 tctcgttata actttattct tttacgctat ctgatcatct tcagcgtggt actgtttgta
     3661 cccgcactat ctcattcaca accaaccgaa agcgaaacac cggagcaagt cattaatgaa
     3721 ttacaagcta cgctgattca agtaatgcaa aaaggcactg aattaggtta tcaagaacgc
     3781 ttcaatcttc tcgcaccagt agttcaacga tctcacgatt taccagcgat tattaaggct
     3841 gtgcttggaa cacactggaa taaattaacc gaggaacaac atcaagcaat taccgaaacg
     3901 tttagtaagc ttagcatcgc cacctatgca gagcgattta atcaacatga tcaagagcga
     3961 tttgagttta ttgaaaaacg taccttaccg aaagagcaag tacttgttcg aagccagctc
     4021 atacaggctg atggcaaagc agttaacttt gattatgtaa tgcgccaagg ggccagtcat
     4081 cattggaaaa ttatcaatat tctcgcggat ggtgtgagtg atctcgcatt aaaaagagca
     4141 gaatacagtg ccatcatcca acgcgatggc ttctctgcac tactttcctt gttagaacaa
     4201 aagattattc aggcagagca acatcactag tatcttctaa tacatgcaat attgttcact
     4261 aacttagcct ttacgtctgt gatcgtggtg cctgacttct catttactaa ttgagacaac
     4321 tttcctttct ttcagtataa cgaactcagt gaaacctacc gatcaacaag aacgtgtatt
     4381 gatagcctgg gcagcgtttt gctatcgcta cgctttttgg gttttgtgtt ttttactcat
     4441 aaccgggatc gcatgcggca cttataccgc gcgtaattta gaagtgctaa cggatacggc
     4501 tgatatgtta tccgaagaac ttccttttcg tgtcaacctt gccaaactca ataaagcttt
     4561 tccacaatac gaaaatacct tgattctggt gctcgatgca cccacacctg aacaagctca
     4621 tgccgcagca agccgattaa cgtcttattt aaaagacgga catatcgagg cttatgatat
     4681 ttattatgga gcgggagaac cattcctaga acagaatggc ctactttata aaaacatcgc
     4741 agagcttgaa tttttagcag accggcttgc ggcagcgcaa ccgctaattg ttagaatcgc
     4801 tcaaaaccca agcctattaa ccttcattca aaccctgaca gaagctatcg atgcatttcg
     4861 tagcggcaac aacctcgaat tagacccagt tctagacgga atttcagcaa ccttgactgc
     4921 aaggctcgcc catagcccta ggccgctttc ttggcaagca ttatttaacg atgagcaccc
     4981 tcaaagcagc tacaaagaaa tcattatcct acaacccaaa ctagattaca ccgaactctt
     5041 tgcagctaaa gattcgattg atgcgataag agccgcattt cagacactta atcttgaaaa
     5101 tgatttttta acaaaactgc ggattacggg tgaagttgca ctttcctatg aagagttgct
     5161 gagtgccatg catggcgctg aatatgcggg tttggttgcg ctcatcatcg ttacgcttat
     5221 tctttttgtt gctttacgat cagcgggggc catcattacc gtgctagtga gcttactatt
     5281 gggattagtc atcacagcgg ctttcacgac ggcaacgatt ggtcatctca atttaatttc
     5341 aatcgctttt gctgcgctct atattggact tgcagtagat tacgccattc atttgatact
     5401 ccgctaccac gagttgaatc gaccagatca acccgtcatc gaaatattgc taagcgccat
     5461 tcacgatatt ggcccatctc tgctaatttg cgcacttaca accgcaattg gattctatgc
     5521 ctttattcca acaacttttc taggaatcgc tgaactggga atcatatcgg gaactggtat
     5581 gtttatcagc ttattcataa cgttaacggt cgtccccgca ttacaacgat ttctgccctc
     5641 caaaatttac tctcaagtac gtaaaaatag atcaataact cagcaatggc tagcgcttcc
     5701 acaaaaatgg cctaaaacag tttatgtgtt agtagcacta tcttttgcac ttgcactcac
     5761 cacatggaat caaattagat ttgattataa tttactgaat ctcagcgacc ctaatggtga
     5821 agctgtgtta acttttcgcg agttactcgc cgatacgcaa cattcaccct ggctatcgat
     5881 ggcaactgtc aaaagtcttg aagaggctaa taaccttgct acgcagttaa caaacctacc
     5941 tgtcgtcgat aaagtgatta gcatcgctga tttcatccca gatcaacaag atgagaaact
     6001 tcaaatcatt gaaggaattg cactcacact cggaccttcc ttctttactg aaaaccatgt
     6061 cgagaatgat gatcttgagt ccagtgattt cacccagatt aaccaagcgc ttgaaaattt
     6121 aaggtatcag ctcaatcaat taatgattga aaaacctaat cacttaagta ttgagtcatg
     6181 gaaaagactg agcttttcga ttgatcaact acttaccttt atccacacag ttcctgctgg
     6241 acaagtgcaa cctttcttaa atgcacttca aaatgattta atcggtcaac tgcccggtgt
     6301 actcaatcgc ctccagcttg ctcttgaagc tagcaccatc aaggatgaat ctttaccaag
     6361 ctcattacgc cagcgctggc acaatgaatc cgacgatttc cttgttgctg tttacccatc
     6421 agaaaacctc aatgataacg atgcactgaa acgtttcatt cgatcggttc aacaacatgc
     6481 accacaaatt agtggcacac cagccatcat gctcgcagca ggagaagcgg tggttgacgc
     6541 atttatccat gcattcattc ttactttact tggtgtaacc ctatcacttt atgtttt
//
LOCUS       DAOQKQ010000152         6581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_5879_length_6581_cov_5.554704, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000152 DAOQKQ010000000
VERSION     DAOQKQ010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 6581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6581
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5879_length_6581_cov_5.554704"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..2394
                     /locus_tag="PKZ49_09775"
     CDS             1..2394
                     /locus_tag="PKZ49_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HND36892.1"
                     /translation="MLPLKPAQLYQACDPSQFNFRTTAELGDLTEIIGQTRAMDAVRF
                     GSSIRQEGYNLFVMGSPGMGKRSLVQQFLKNKASREAEPADWCYLNNFDQPHQPKVLK
                     LPPGKGEALRLSMEGLVEYLRTAIPAQFESEEYRSKVNAIHQAFNERQEKVISELGEE
                     AEKQEIVLLRTPDGFALAPSRNHEVIPPEEYDKLPQAEKERILTAITGLQARLEKVLN
                     HLPQWRRERSEHIKQLNRDIMLSVVAHVIDELRTQYANLPEVLSFLDAVQRDMILHVD
                     DFRKPDESMNISGIPVVAHQTFSNYKVNVLVSNQNSSGAPLVCEDNPTYSNLLGRVEH
                     ISQFGALVTNFTLIKPGALHRANGGYLLLDIRKILMQPFAWDALKRALQTREIRIESL
                     GQMYSLISTVSLEPEPIPLAVKVVLFGDRLLYYLLQAYDPEFSELFKVAADFEEVIDR
                     SAEVQQLYAQLIATLVRKESLLPYDRDAVARVIEHSSRLVSDSEKLSMQMRNIADLLR
                     EADYWARDAGHATVLASDVQHAIDAQIHRQDRIRDRLYEAILRGTLMIDTAGAVIGQV
                     NGLAVIELGSFAFAQPTRITATTRLGKGDFINIEREAELSGAIHSKGVLILAAFLASR
                     YAKNQPLALSASLAFEQSYGKIDGDSASLAELCALLSNLAHVPILQTLAITGSVNQLG
                     QAQAIGGVNEKIEGFFDICVARGLSGQQGVVIPVSNVKHLMLRRDVIDAIAKGLFAIY
                     AVENVDQAISLLTGISAGEVDEKGIYPKGTINDLVIERLAELTKITQSLSSIEKGEK"
     gene            2484..3131
                     /locus_tag="PKZ49_09780"
     CDS             2484..3131
                     /locus_tag="PKZ49_09780"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HND36893.1"
                     /translation="MRILSLPNFWLLIALVSLIIIYPIAYPYSSGRAFLALFDWIILI
                     LALRASKKTWYKSWLGYAFAIPAMSFYVIAALFSSSVVIAASLVTQALFHGFIVISLL
                     RYMLHDNVMTLDELFAAAALYVLIGFVFGYCYVLIEHIHPGSFYINEANNPDQIVSWW
                     ELLYFSFTCLTSVGFGEITPISDSARSIVIIEQTSGVLYLAILISRLMSMRMKRE"
     gene            complement(3161..3754)
                     /gene="scpB"
                     /locus_tag="PKZ49_09785"
     CDS             complement(3161..3754)
                     /gene="scpB"
                     /locus_tag="PKZ49_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HND36894.1"
                     /translation="MSDQFSLTLPVSQSTSFSNQADQIRILEAALLTSQEPLPIAELK
                     KLFDGTINSKQLLTMLDELSNQWQEKGIELVEVASGWRFQSRPEMQVYLEKLNPQKPP
                     RYSRAVLETLAIIAYRQPVTRGDIEEIRGVTVSSAILKTLETRGWIEVVGQRNVPGRP
                     QLYATTSNFLNDLNLQSLDQLPPLDELVNQTEHATDE"
     gene            complement(3738..4529)
                     /locus_tag="PKZ49_09790"
     CDS             complement(3738..4529)
                     /locus_tag="PKZ49_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HND36895.1"
                     /translation="MDELNSLVEAHPIAKICGQPLHEVPHDLYIPPEALEIFLDAFEG
                     PLDLLLYLIRKHNLDILDIPMAELTQQYMAYVEAMRADQLELAAEYLLMTALLIEIKS
                     RMLLPKPIHTPEEEADPRAELVRRLLEYEQMKKAALRLDELPQLERDFMQVQVLINQT
                     VIQQLVQVNLDDLQQAWLTLLQRAKLSTRHHVQFEAISVRSCMSEILRKLKQAGTVSF
                     YDLFSPSPAIGEVIANFLAILELAKELLIEIEQTEVFGTIYVRSI"
     gene            complement(4579..5781)
                     /locus_tag="PKZ49_09795"
     CDS             complement(4579..5781)
                     /locus_tag="PKZ49_09795"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HND36896.1"
                     /translation="MFADRVLSGMRPTGHLHLGHYHGVLKNWVTLQQQYECLFFVADW
                     HALTTHYDSPEMIEQHTWEMIIDWLAAGVDPAQATLFIQSKVPAHAELYLLLSMITPL
                     GWLERVPTYKDQQEKLTEKDLSTYGFLGYPLLQSADILIYRANRVPVGEDQAPHLEFT
                     REISRRFNHIFGKEPGFEDKAEQAIKKLGSKRSKLYRELRTRYQEQGDESALEEGRSL
                     LDEQQNLSLGDRERLFGYLEGGGRVILAEPETLLTQASRMPGLDGQKMSKSYDNTISL
                     REDADTIRKKIRTMPTDPARIRRTDPGDPAKCPVWSFHAVYSNDEVRNWVQQGCKNAS
                     IGCLECKQPVIDAILAEQIPICERAKMYEEDPALVRNIVADGCEKANKLAEETMRDVR
                     QVMGLSYS"
     gene            complement(5825..6502)
                     /locus_tag="PKZ49_09800"
     CDS             complement(5825..6502)
                     /locus_tag="PKZ49_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HND36897.1"
                     /translation="MENFDSIIQGIAIYALPVIFAITFHEAAHGYIAKHFGDLTAYQA
                     GRISLNPIKHIDPMGTIIVPLTLFIIGKLTMGTGFLFGWAKPVPVNFGQLHHPKRDML
                     WVAAAGPIANLFMACCWAVVLKLGALSFPDNFYAKPMILMGKAGIDINLILMVLNLLP
                     LPPLDGGRMAISLLPNRLAYMFSRIEPYGFMILLFLLITGILGAIVWPFIVFMKYAIA
                     WLFGLYI"
BASE COUNT         1782 a         1357 c         1492 g         1950 t
ORIGIN      
        1 attcttcctt taaagccagc acaactctat caagcgtgtg atcctagcca gtttaacttt
       61 agaacgacgg ctgaactagg ggatttgact gaaataatcg gtcaaacacg cgccatggat
      121 gcggtgcgtt ttggttcaag tatccgacaa gaaggttata acctttttgt aatgggttct
      181 ccggggatgg gtaagcgtag tctggttcag caatttctta aaaataaagc cagccgagaa
      241 gctgagcctg ccgattggtg ctatctcaac aattttgatc aaccgcacca gcctaaagta
      301 cttaaactgc ctccaggtaa aggggaggca ttgcgattat ccatggaagg gctggtggaa
      361 tatctccgca ccgcaattcc tgcacagttt gaaagcgaag aatatcgcag taaagtcaat
      421 gcaatccatc aagcgtttaa tgaacgccag gaaaaagtga ttagcgaact gggggaagaa
      481 gcggaaaaac aagagatcgt attgctgcgt acgcccgatg ggtttgcttt ggcaccgagc
      541 cgcaatcatg aagtgattcc acccgaggaa tacgacaaac tccctcaagc agaaaaagaa
      601 cgtattttaa cggcgattac tggactacaa gcgcgcttgg agaaagtgct taatcatctt
      661 ccgcagtgga gaagggagcg cagcgagcat attaagcagt tgaaccgaga tatcatgctt
      721 tcagtcgttg cccatgtcat tgatgaacta cggacacaat atgctaattt gcccgaagtg
      781 ctgagctttc tcgacgcagt gcagcgcgac atgattttgc atgtcgatga ttttcgtaaa
      841 cccgatgaat cgatgaatat ttcaggtatt ccagttgttg cccaccaaac cttttccaat
      901 tataaagtca atgtgttggt ctcgaatcaa aattcctccg gtgcgccttt agtctgcgag
      961 gataatccaa cttatagtaa tttgcttggc agggtagagc atatttcaca atttggcgca
     1021 ttggtaacaa actttacctt gattaaacca ggtgcattgc atcgagccaa cggtggttat
     1081 ttactgctgg atatccgcaa aatactcatg cagccgtttg cctgggatgc attaaaacgc
     1141 gctttacaga cacgcgagat ccgcattgag tcattaggcc agatgtatag cttgattagt
     1201 accgtttcgc tggaacctga gcctattccg ctagcggtaa aagtggtttt atttggcgac
     1261 cggctacttt attacctgtt gcaggcttat gatcctgaat ttagtgaatt gtttaaggtt
     1321 gctgcagact ttgaagaagt tattgatcga agtgcagaag tacagcagtt gtatgcacag
     1381 ctcattgcaa cgttagtgcg taaggaatcc ttattgcctt atgatcgtga tgccgttgca
     1441 cgggtcatcg agcatagctc gcgtttagtc agtgactcag aaaagctttc catgcagatg
     1501 cgtaacatcg ctgacttgtt acgagaagcc gattattggg cacgagatgc gggtcacgca
     1561 accgtgctgg cgagcgatgt acaacatgcc atcgatgcac aaattcatcg ccaagatcgt
     1621 attcgggatc gcttatatga agcaattcta cgcggcactt tgatgattga tactgccggt
     1681 gctgttattg gacaggtcaa tggattggcg gtcatcgaac tcggtagctt tgcttttgct
     1741 caaccaacac gcatcacagc aacgactcga ctcggtaaag gggattttat caatatcgag
     1801 cgagaggcgg agctttcagg cgcaattcac tcaaaaggcg tgttgattct ggctgcattc
     1861 ttggcctcgc gctacgctaa aaatcaacca cttgcactat cagcgagtct ggcatttgaa
     1921 caatcctacg ggaaaatcga tggtgatagt gcttcactcg cagaattgtg tgcattgctt
     1981 tctaatttag cgcatgtacc tattcttcaa acgctggcga tcaccggctc tgtcaatcaa
     2041 ctcgggcaag cgcaagcaat tggcggtgtg aatgaaaaga ttgaaggttt ttttgatatt
     2101 tgtgtcgcac ggggcttatc gggtcaacaa ggagtagtga ttcctgtttc aaatgtaaag
     2161 catttaatgt tacggcggga tgtgattgat gccatcgcaa aagggctttt tgcaatttat
     2221 gccgtggaaa atgtcgatca agcgatttca ttattgactg gtatttccgc aggtgaagtt
     2281 gatgagaagg ggatttatcc caaaggaacg atcaatgatt tagtcatcga acgcttggct
     2341 gagctgacta aaatcactca atcattatcg tcaatagaaa agggtgagaa ataatcagtt
     2401 aatttttctc gatcaagctt aaaaattaag cttatttgtg tatagaattc cttaacgctt
     2461 taaccattaa aataagatag cgcatgcgca ttttgtctct ccccaatttt tggttactga
     2521 tcgcgctggt ctcgctcatt atcatttatc ctattgccta tccctattct agtggtagag
     2581 cctttctcgc gttattcgat tggatcatat tgatccttgc attacgtgct tcgaaaaaaa
     2641 cctggtataa atcgtggctg ggatacgcgt ttgcgatacc ggccatgtca ttttacgtca
     2701 tcgctgcatt atttagctca tctgttgtta tcgcagcaag cttggttacg caggcattgt
     2761 tccatggctt tatcgtgatt agcttattgc gctatatgtt gcacgataat gtgatgacac
     2821 ttgatgagct ttttgctgcg gctgctttgt atgtattaat aggatttgta tttggttact
     2881 gttatgtttt gattgagcat attcatcccg gtagttttta tattaacgaa gcgaataacc
     2941 cagatcagat cgtatcttgg tgggagctgt tgtatttcag ttttacctgt ttgacgagtg
     3001 ttggttttgg tgaaattacg ccaatctcag attcagcgcg ttcgattgtg attatcgagc
     3061 agacatcggg tgtattgtat ttagcgattt taatttctcg tttgatgtcg atgcgcatga
     3121 agcgggagta ggtgtgctgg ttttcgatcg ttttcgctac tcattcatct gtcgcatgtt
     3181 cagtttgatt gaccagctca tctaaaggcg gtagttgatc cagtgattgg aggtttaagt
     3241 cattcagaaa attagaggtg gttgcataga gttgcggtct tccaggaaca ttacgttgcc
     3301 cgacaacttc gatccaaccc cgtgtttcca gtgttttgag tatcgcactg gaaacggtga
     3361 cacctctaat ttcttcgatg tcaccgcggg ttaccggttg acgataagcg atgattgcaa
     3421 gggtttccaa tacagcgcgt gaataacgtg gtggtttttg tggattaagt ttttccagat
     3481 aaacttgcat ttcgggtcga ctttggaagc gccatccgct tgcaacctct accagttcaa
     3541 tccctttttc ttgccactga ttgcttaatt cgtccagcat cgtgagtaat tgtttactat
     3601 tgatggtgcc atcgaatagt ttttttaact cagcaatcgg cagtggttcc tgagaagtca
     3661 ataacgctgc ttccagaatt cggatttgat cggcttgatt ggaaaacgaa gtagattgag
     3721 aaacaggtag agtcaggcta aattgatcgg acatagatgg ttccaaaaac ctcagtttgt
     3781 tcgatttcaa tgagtaattc ttttgctaat tcaaggatgg caagaaagtt cgcaattact
     3841 tcacctatgg ctgggctagg tgaaaataaa tcgtaaaaag aaaccgttcc agcttgcttt
     3901 agtttccgca aaatttcact catgcaagag cgcacggata ttgcctcaaa ctgaacgtga
     3961 tgccgcgtac tcagttttgc tcgttgaagc agagttaacc atgcctgctg taaatcatca
     4021 aggttgactt ggacaagttg ctgaataact gtttgattaa tcaaaacttg cacttgcatg
     4081 aaatcgcgct cgagttgcgg taattcatcc aagcgaaggg ctgctttttt catttgctca
     4141 tattctagca aacgacgtac aagctcggca cggggatctg cttcttcttc aggggtatga
     4201 atcggtttag gaagcaacat gcgtgattta atctcgatta gcaatgcagt cattagcaaa
     4261 tattctgccg ctagctctaa ttgatcggca cgcatcgcct caacataggc catatattgc
     4321 tgggttaact cagccatggg gatatctaaa atatccaagt tatgtttgcg aattaagtaa
     4381 agcaataaat caagcggacc ttcaaacgca tcaagaaaaa tttctaatgc ttcaggtggg
     4441 atatataagt catgcggtac ttcatgtaac ggttgaccgc atatttttgc aatggggtga
     4501 gcctcaacga gtgagtttag ctcatccata ccattggctc ctgtgctaaa tcaattaatt
     4561 aatggttaac gaaaatgatc aagaatagct taatcccatg acctgcctaa catcacgcat
     4621 agtttcttct gctagtttat tggctttctc acagccatcc gcaacgatat ttctcactaa
     4681 ggctggatct tcttcataca ttttagcgcg ctcacaaatt gggatttgtt ctgctaagat
     4741 ggcgtcaata acgggttgct tacattctag gcaaccaata cttgcatttt tacacccttg
     4801 ttgaacccaa ttcctgactt catcatttga ataaaccgcg tgaaatgacc aaactggaca
     4861 tttcgcgggg tcaccagggt cagtacgacg gatgcgagcg ggatcggttg gcatggtgcg
     4921 aatttttttg cggatagtat cagcatcttc acgtaaactg atcgtattat catatgattt
     4981 agacattttt tgcccgtcta accccggcat tcgcgatgct tgggtcaata atgtttcagg
     5041 ttcggccaaa atgactcttc ctcccccttc taaataacca aacaatcttt ctcgatcacc
     5101 taagcttaaa ttttgttgtt catcgagtag tgaccgccct tcttctaatg cactttcatc
     5161 gccttgttct tgatagcgcg tgcgtaattc acggtaaagc ttggagcgct tcgatcctag
     5221 ttttttgatg gcttgttcgg ctttatcttc aaacccgggt tctttaccaa aaatatgatt
     5281 gaagcggcgg gaaatttcgc gcgtaaattc gaggtgaggt gcctgatctt caccaacagg
     5341 aacgcgattg gcgcgataaa taagaatatc tgcactttgc agtaaaggat agcctaaaaa
     5401 tccatacgta cttaaatctt tttcagtgag tttttcttgt tgatctttat aggtaggaac
     5461 gcgttcaagc caacctaatg gcgtgatcat ggaaagcagt aaatagagtt ctgcgtgggc
     5521 aggtactttg gattgaatga acaatgtggc ttgcgctggg tcaacgcctg cagctaacca
     5581 atcgatgatc atttcccaag tatgttgttc gatcatttcg ggtgaatcat aatgcgtggt
     5641 taatgcatgc caatctgcca caaaaaacaa acattcatat tgttgttgta gcgttaccca
     5701 attctttaga acaccatggt aatgtcccaa atgcaaatga cccgtagggc gcatccctga
     5761 taaaacacga tcagcaaaca tgatcaattt tccaaattaa tgtgtgaaag attaaagttt
     5821 ctcattaaat atataagcca aatagccatg caatcgcata cttcatgaat acgataaaag
     5881 gccaaacaat ggctcccagt ataccggtga ttagcaaaaa taaaagaatc atgaagccgt
     5941 agggttcaat acgcgaaaac atatatgcta gtcggtttgg gagtaagcta attgccatac
     6001 ggccaccatc gagtggaggt aggggtaata gatttaacac cattaagata aggttgatat
     6061 cgatccctgc cttacccatt aatatcatcg gttttgcgta aaagttgtca ggaaaagaga
     6121 gtgcacctaa ttttagaacg actgcccaac agcatgccat aaaaagattt gcaatgggtc
     6181 cggctgcagc aacccacaac atatcgcgtt tcggatggtg taattgacca aaattgactg
     6241 gaactggttt tgcccaaccg aataaaaagc ctgttcccat ggttaatttc ccaatgataa
     6301 acaaggttag agggacaata atcgttccca ttgggtcaat atgcttgatt ggatttaaac
     6361 tgatgcgtcc agcctgataa gcagtcaaat caccaaagtg tttggcaata tacccatgag
     6421 ccgcttcgtg gaatgttatg gcgaaaatga cgggtagtgc gtaaattgca atcccttgga
     6481 tgatactgtc aaaattttcc attagctggt caatccaaag ggctttaggc ttccttttcc
     6541 ttgcctaatc agtgctggtg tatcggttgt tagatcaatg a
//
LOCUS       DAOQKQ010000153         6514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_5977_length_6514_cov_5.263663, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000153 DAOQKQ010000000
VERSION     DAOQKQ010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 6514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6514
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5977_length_6514_cov_5.263663"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2024)
                     /locus_tag="PKZ49_09805"
     CDS             complement(<1..2024)
                     /locus_tag="PKZ49_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HND36898.1"
                     /translation="MALAWNEIKDRALAFSRDWAKAESEDADAKPFWIEFFHIFGITS
                     RRIGSFEQKVKKLDGRDGYIDWLWKGNLLIEHKSRGKDLDRAYQQAIDYFPGLKEHEL
                     PRFVLVSDFARFRLYDIVEGTHSEFLLKELHKNVKLFWFIAGYQPQRILPESPINSEA
                     VKRMARLHDRLKQIGYTGHALEVYLVRLLFCLFAEDTGIFERTQFTEFIGQNTREDGS
                     DLASQLAQLFDVLNTPEDARLRNLDDTLSRFPYINGKLFEERLPFASFDRDMRAALLY
                     CCAIDWSGISPAIFGSLFQNIMDETPNARRNLGAHYTTEENILKLIGPLFLDELRAEF
                     DKIRHTVKSRTQNLQAFHAKLAGLKFLDPACGCGNFLVIAYRELRKLELEVLRELHHS
                     GQQALDIATIIQVDVDQFHGIEIEEFPVQIAQVALWLTDHQMNALVSEEFGQYFIRLP
                     LKKSAHIVHGNALRLDWNSVIAAEECDIVMGNPPFIGYAYQNKEQKADLQNIFAAIKG
                     AGVLDYVAAWYVKATEYMSINPIINAAFVSTNSITQGEQVGILWPELLKRNVKIYFAH
                     RTFQWSSEASGRAAVHCVIIGFSLQEVAHKIIFEYENIQGEPQAVSVRNINPYLVDAP
                     IILLAKRQAQICNAPEINKGSQPTDGGNLLLNDSEKQELLESDPRAEKWLR"
     regulatory      2359..2470
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00442"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="guanidine-I (ykkC/yxkD leader) riboswitch;
                     Guanidine-I riboswitches control genes whose products are
                     involved in modification or pumping out guanidine as a
                     toxic compound from bacteria; This is class of guanidine
                     riboswitches is based on the ykkC/yxkD leader; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF00442"
     gene            2527..2709
                     /locus_tag="PKZ49_09810"
     CDS             2527..2709
                     /locus_tag="PKZ49_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36899.1"
                     /translation="MPSHEKHVIFFAVSSVTEPNTDKKSRYPTQIPIRESESVRCIFY
                     VIVISLELWFFYIIEE"
     gene            2714..4156
                     /locus_tag="PKZ49_09815"
     CDS             2714..4156
                     /locus_tag="PKZ49_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HND36900.1"
                     /translation="MEHSQKSKQPLPSASINPGETLNRNMGIWNSFTLGFAVVSPVVG
                     LYAIVGVQAQVTGGGWFAALIICLAMQLFVATVYAELASQFPIAGGAYKWAHLLCGRI
                     TGQYAGAIYVSSTIAMLTTTAYTGGIWLAILFGLNSESGIAMVGWGIAFLLLCLAINL
                     APVKFFKGIIAMGVYAEIVGSFGMALLLFLFFRELPFSELFQHLGTGTAPNQLSAFLA
                     ALAIAGWAFIGFDACSTTSEETHQPKHSVPRAVFLSLLAVGTVVVFNSAALTLSVDHE
                     VLSKTSSTFDPITPLISTHIGAWAEKPFLLIVVIAFLACGASVVKYTSRIVFSMAREE
                     ILPSQLSIVSHHGTPHHAILATVVFSSMGLMLGLSDEAVATIISFGTGGLYAMFATTT
                     GVALFSRLTGRWNPALGELKLGIWGLIINSIAFLWSVFELINIAWPRSYMAAPDAPWW
                     QLWAVPLVLAGILVLTTLVILIKGEKHGTQ"
     gene            4143..4871
                     /locus_tag="PKZ49_09820"
     CDS             4143..4871
                     /locus_tag="PKZ49_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HND36901.1"
                     /translation="MEHNDIDWKLIIPGGCHWSGILRRGTTLRLTDIDGNANVAALFY
                     NQEEKLERYNMADTLKAQHTFYLTQGHACYSDMGRIFCCITADTAGWHDTVCGLSNAK
                     LIEQKYGTARYQAHRNQMFRSGLDGILIELGKWGLGLRDVVANINFFSKVTANQEGQL
                     TFQRDHRSPNASVDLSFEMNTLVVLSMAPHPLDTAPTYAPGPVQLSIFPTQPTTQWSC
                     SQRPENIRGRHNTQQFYCLHGGTQ"
     gene            4868..5539
                     /locus_tag="PKZ49_09825"
     CDS             4868..5539
                     /locus_tag="PKZ49_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HND36902.1"
                     /translation="MSHQQYIESYLTPDTAIIDEICPAGEPWSKVIQHGQIFRIVDLE
                     GNQAVDTLFFNAHAPQERYSAADTIRNQSNVYLTTNSQLYSNVGNILLTIIADTCGRH
                     DTLGGACAAESNTVRYALEKFPMHSCRDNFLYALATEPIFNQLGMNKRDLPSNINFFM
                     NVPVTESGDLTFSDGVSAPGKYVEMRAEMDVLVLISNCPQLNNPCNAYNPTPIRLLVW
                     DRPCA"
     gene            5579..>6514
                     /locus_tag="PKZ49_09830"
     CDS             5579..>6514
                     /locus_tag="PKZ49_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HND36903.1"
                     /translation="MFKKVLIANRGAIACRIMRTLRRLNVASIAVYTEADHLSRHVDE
                     SDEAYCIGNGAVAESYLDINKILAIAKQAQAEAIHPGYGFLSENPNFAEACAQQGIRF
                     IGPTPEQIRIFGLKHTARSIAQQNQVPLLPGTDLLSDVEAACRHAHTIGYPVMLKSTA
                     GGGGIGMRLCADEAELINHYEAVKKLAASHFKESGLFLEKYIAQARHIEVQIFGDGKG
                     GVIALGERDCSLQRRNQKVVEETPAPNLSPAIRSALLNTAVKLGQAIHYESAGTVEYI
                     FDTQTQQFYFLEVNTRLQVEHGITEAVTGVDLVEWM"
BASE COUNT         1650 a         1600 c         1498 g         1766 t
ORIGIN      
        1 cgtagccatt tttctgctct tggatcagat tccaataatt cctgtttttc cgagtcattt
       61 aataacaaat tgccgccatc tgtgggttgg cttcctttgt taatttcagg cgcattgcaa
      121 atttgtgctt gacgttttgc aagtaaaata ataggcgcat ctactaaata ggggttaatg
      181 tttcttactg aaactgcttg aggttcgcct tgtatatttt catactcaaa aataatttta
      241 tgtgcaactt cctgcaagct gaagccaata atcacacaat gcactgccgc tctaccgcta
      301 gcctcgctgc tccactgaaa cgtacggtgc gcaaaataaa tttttacatt tctcttcagt
      361 aattctggcc ataggatgcc gacttgctcg ccttgcgtga tcgaattggt ggaaacaaat
      421 gctgcattaa tgattggatt gatcgacatg tactcagtgg ctttaacata ccaagcagca
      481 acataatcca gtactccagc acctttgatg gcagcaaaaa tattttgtaa atccgctttc
      541 tgctctttgt tttgataggc gtaaccaatg aacggcggat tccccatcac aatatcgcat
      601 tcctctgccg cgatgacgct gttccaatcg agccgcagtg cattgccgtg cacgatgtgc
      661 gcggatttct tcagtggcag gcggatgaaa tactggccga attcttccga caccagcgcg
      721 ttcatctggt ggtcggtcag ccatagcgcg acttgcgcga tttgcaccgg gaattcctcg
      781 atttcgatgc cgtgaaactg gtcgacgtcg acctgaatga tggtggcgat gtcgagcgct
      841 tgctggccgc tgtggtgcag ttcacgaagc acttccagct ccaatttgcg cagctcacgg
      901 tacgcgatca ccaggaaatt gccgcagccg catgccgggt cgagaaattt cagcccagcg
      961 agcttggcgt ggaaggcttg caggttttgc gtgcggcttt tgacggtgtg acggatttta
     1021 tcgaactcag cgcgcaattc gtcgaggaac agcgggccga tgagtttcag gatgttttcc
     1081 tcagtggtgt agtgcgcgcc gaggttgcgc cgcgcgttcg gcgtttcgtc catgatgttc
     1141 tggaacagcg agccaaaaat cgccggggag atgccgctcc agtcgatggc gcagcaataa
     1201 agcagcgcgg cacgcatgtc gcggtcaaag ctggcgaacg gcaggcgttc ctcgaacagt
     1261 ttgccattga tgtacggaaa tcggctcagc gtgtcgtcga ggtttctcag ccgggcgtct
     1321 tccggcgtgt tgagcacgtc gaacagttgc gccagttgcg acgcaagatc gctgccatcc
     1381 tcacgcgtat tttgtccgat aaattcagtg aactgagttc gctcaaaaat cccggtatcc
     1441 tcggcgaaca agcagaacaa caaacgtacc aaatacactt cgagcgcatg gccagtgtaa
     1501 ccgatctgct tcaggcgatc gtgcaaccgc gccatgcgct tcactgcttc gctgttgatc
     1561 ggactctctg gcaaaattct ctgcggctgg taacccgcga tgaaccagaa cagcttaacg
     1621 tttttgtgta gttctttaag caaaaactcg ctatgcgtgc cttcgactat atcgtaaaga
     1681 cgaaagcgcg caaagtcgct gaccagcacg aagcgcggca gttcgtgttc cttcagtcca
     1741 gggaaataat cgatggcttg ctggtaggcg cgatcgagat ctttaccacg cgatttgtgt
     1801 tcaatcaaca ggttgccttt ccacagccaa tcgatgtaac cgtcacgacc atcgagcttt
     1861 ttgaccttct gttcgaaact accaatacgc cgcgaagtaa taccgaatat atggaaaaat
     1921 tctatccaga acggtttggc gtcggcgtct tccgattccg ctttggccca gtcgcgagaa
     1981 aatgctaatg cgcggtcttt gatttcattc caggccagtg ccatttgttc tccaagtaat
     2041 agctataaat tgattaagtt tgtcggatgt ctgattttag atcgtcaccg cagcattata
     2101 ataactacga agctattttc ttagtatgaa acatttctgg cacatatctt tgggccataa
     2161 tatatgtgtg atgcggcact caaataaaac cccaaataat tcaaagcaat aaaattagtt
     2221 gatatttccc tcaaaactcg ttaaattcac agccaatgca tgtggttaat atgagtaaat
     2281 cctaaaccat tgcatcgaat agttttttag agagacgacc ttaaaatcca atcagtgcat
     2341 caaaccttac tgattggtgt tatagcaaac tagggttccg actactttcg ataaaggaag
     2401 aagtgctggt ccgagagttt ctggcttcgt tcataacgaa gctccacgga gggataaaag
     2461 cccgggagat cgcaagtgtt ttacacatta cgactctctg tattttattt aaatggagct
     2521 ttagttatgc catcacatga aaagcatgta attttctttg cagtttcatc agtaacagaa
     2581 ccgaataccg acaaaaagtc acgttatccg actcagattc caatacgaga atcagaatcg
     2641 gtgcgttgca ttttttacgt catcgtaatc tccctggagc tctggttttt ctacatcatt
     2701 gaggagtaat gcaatggaac attcacaaaa gtctaaacaa ccattgccta gcgcttccat
     2761 caatcccggc gaaacactca accgaaatat gggtatctgg aatagcttta cactcggttt
     2821 tgccgtggta tctcccgtcg ttgggcttta tgcaatcgta ggcgtgcaag cgcaagtaac
     2881 cggtggtggt tggttcgcag cacttataat ctgtcttgct atgcaattat ttgtggcaac
     2941 cgtttatgcc gaactcgcct cgcaatttcc aattgccggt ggcgcctata aatgggctca
     3001 tttactgtgc ggtcggatca ctggccaata cgcaggtgcc atttatgtca gctcaactat
     3061 cgcgatgtta acgaccacag cttatacggg tggaatctgg ttagcgatcc tctttggctt
     3121 aaacagcgaa tcaggtattg cgatggtcgg gtggggtatt gcctttttat tgctatgcct
     3181 tgcaatcaac ctggcgccag ttaaattttt taaaggcatc atcgccatgg gcgtctacgc
     3241 agaaatcgtc ggctcttttg gaatggcact actactattt ctattcttta gagagctacc
     3301 cttttctgag cttttccagc acttaggaac gggcaccgca cccaatcagc tatccgcttt
     3361 tttagcagct ttagcaatag cggggtgggc attcattggc tttgatgcat gttcaaccac
     3421 atcggaagaa actcatcagc ctaaacattc cgtcccccgg gccgtgttcc tctccctcct
     3481 tgccgttggc accgtagtag tttttaattc tgccgcatta actttatcgg tggatcatga
     3541 agtgctttct aaaaccagtt caacctttga tccgattaca ccgctcatct caactcacat
     3601 cggcgcctgg gctgagaaac cctttttgct gatcgtcgtt atcgctttct tagcttgtgg
     3661 cgcatccgtt gtcaaatata cctcgcgcat cgtattctca atggcccgtg aagaaatatt
     3721 accttcccaa ctcagcatag tgtctcatca cggcacaccc catcacgcta tcctagccac
     3781 tgtagtattt tccagtatgg gcttaatgct tggactcagc gatgaggccg ttgcaacgat
     3841 tatttcattc ggcactggcg gattatatgc gatgtttgca acgacaaccg gcgttgcttt
     3901 attcagtcgc ctcacgggac gttggaatcc tgctttaggg gaactcaaat tagggatttg
     3961 gggtttaatc attaacagta tcgccttctt atggtctgtt tttgaactca tcaatattgc
     4021 ttggccacgc tcatatatgg ctgcacctga tgccccttgg tggcagcttt gggcagtacc
     4081 gctcgtttta gctggcattc tggtgctaac aaccttagtt atcttgataa aaggagaaaa
     4141 acatggaaca caatgacatc gattggaaat taatcattcc aggaggctgc cattggtcag
     4201 gcatcttgcg ccgtggcact acgctaagat taaccgatat cgatggaaat gctaacgtag
     4261 cagcactctt ctataatcaa gaagaaaaac tcgagcgtta caacatggca gatactttaa
     4321 aagctcagca taccttttat ctaacgcaag gccatgcctg ctactccgat atgggaagaa
     4381 ttttttgctg tatcacagcg gatacggcag gatggcacga tacggtttgt gggctaagta
     4441 atgccaagct tattgagcag aaatacggta ccgctcgcta tcaagcgcat cgcaatcaga
     4501 tgtttcgcag tgggttggat ggaatattaa ttgaacttgg caaatggggc cttggtttgc
     4561 gggatgtcgt tgccaacatc aactttttca gtaaagtcac tgccaatcaa gagggtcaac
     4621 tcacttttca gcgtgaccac cgttccccca acgcatcggt tgacctgagt tttgagatga
     4681 ataccttagt cgtgctttcg atggcgcccc accccctcga tacggcgcca acctacgcgc
     4741 ctgggccggt ccaattatcg atattcccca cacaaccgac aacccagtgg tcctgctcac
     4801 agcgtcctga aaatattagg ggtcgacaca atacccagca attctattgc ttgcacggag
     4861 gaacacaatg agccatcaac aatacatcga aagttattta acgcctgaca cagcgattat
     4921 cgatgaaatt tgccccgcag gagaaccgtg gagtaaggtc attcaacacg gtcaaatttt
     4981 ccgtatcgtc gatttagaag gtaatcaggc agtcgacacc ttatttttta atgcccatgc
     5041 accacaagaa cgctacagtg ccgctgacac cattcgcaac caatcgaatg tatatctcac
     5101 gacaaacagt cagctctatt ccaatgttgg aaacatccta ctaaccatta tcgccgatac
     5161 ctgtggtcgc cacgataccc tcggtggcgc ttgcgctgca gaaagtaata cggttcgcta
     5221 tgcgttagaa aaatttccga tgcacagttg ccgcgataat ttcttatatg cgctggcaac
     5281 tgaacccatt tttaatcaac tggggatgaa taagcgcgac ttgccgagca atatcaattt
     5341 tttcatgaat gtcccggtga ccgaatccgg tgatctaacg tttagcgatg gagtttcagc
     5401 accgggcaag tatgtcgaaa tgcgtgctga aatggatgtg ttggtgttga tatcgaattg
     5461 ccctcaatta aacaacccct gcaatgccta taacccgaca ccgattcgct tgctcgtttg
     5521 ggatcgtcct tgcgcctaaa tcacgataac gacaccatca ctcggagaaa tcgtagccat
     5581 gtttaaaaaa gtcctcattg ccaatcgagg tgcgatcgct tgtcgcatca tgcgcacgtt
     5641 acgtcgattg aacgtcgcca gtatcgcggt ttataccgag gcagaccatt tatcgcgcca
     5701 cgtcgatgaa agcgacgaag cctattgcat tggcaacggt gcggttgctg aaagttattt
     5761 ggatattaac aaaattttag cgattgcaaa gcaagcccag gctgaagcga ttcaccccgg
     5821 ttatggtttc ctcagtgaaa accctaattt tgctgaagcc tgtgcacaac agggcatccg
     5881 ctttatcggc ccgacaccgg agcaaattcg aatttttggg ttaaagcata ccgcacgcag
     5941 cattgcgcaa caaaaccaag tgccactatt gcctggtact gatttgttat ccgatgttga
     6001 agcggcctgt cgtcatgccc acacgattgg ctacccagtg atgctcaaaa gtaccgctgg
     6061 cggcggcggt atcgggatgc ggctatgcgc agatgaagcc gaattaatta accactatga
     6121 agcggttaaa aagctggcag ctagccattt caaggaatca gggcttttcc ttgaaaaata
     6181 cattgcccaa gctcgccaca ttgaagtgca aatctttggt gatggaaaag gcggtgttat
     6241 cgcactcggt gagcgcgatt gctcgttaca gcgtcgcaac cagaaagtgg tcgaagaaac
     6301 acctgctcct aacctttcac cggcgatccg ttcagcgcta ttaaatactg cggttaaact
     6361 cggtcaagcc atacactatg aatcagctgg caccgttgaa tatatctttg atactcaaac
     6421 tcagcaattt tatttcctcg aagtcaacac gcgtttacaa gtagaacacg gcatcactga
     6481 agccgtgact ggggtggatc tcgtcgaatg gatg
//
LOCUS       DAOQKQ010000154         6390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_6163_length_6390_cov_5.682242, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000154 DAOQKQ010000000
VERSION     DAOQKQ010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 6390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6390
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6163_length_6390_cov_5.682242"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="PKZ49_09835"
     CDS             complement(<1..654)
                     /locus_tag="PKZ49_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HND36904.1"
                     /translation="MLIEIKVPQLSESVAEATLISWHKKQGEKVVRGENLIDIETDKV
                     VLELPATQDGILIEILKNDGATVKSGEIIAKIETEMTSTASVSSSLTINQGTVKPKPE
                     TKNQIAKILMPAAQKMAEENQLKQNEVNSIVGTGREGRVTKEDIANHIKSKPQTAEVT
                     STPASILSEVPNMEQLASNTERLEQRVPMTRLRMRIAERLVQSQSTAAILTTFNEVNM
                     "
     gene            complement(668..3523)
                     /locus_tag="PKZ49_09840"
     CDS             complement(668..3523)
                     /locus_tag="PKZ49_09840"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HND36905.1"
                     /translation="MMRQFIENSFLFGTNAPFVEDLYERYLSSPESISPEWRSFFDNL
                     QQTIAISTKDRPHSLIIQSLVNPVNKIASIQDKSLTQPLFNQPYDETKERKQIAVLQL
                     INMYRFLGVRCAKLDPLNHQPLLDVPELDPAFHGLTANDLDTNFNTGSLVGPEHAPLR
                     TILQILQQTYCNAIGAEYMFITDLKQKRWIQSRLESTCAQPSYGNEYKKHILERLTAA
                     EGLEKYLHTRYVGQKRFSGEGNESLIPLLDQLIQHAGLMGVQEIVLGMAHRGRLNVLV
                     NTLGKMPSDLFLEFEGKHVQTLMAGDVKYHQGFSSAVMTPGGIVHLALAFNPSHLEIV
                     DPVVEGSVRARQHRLSDKQGQLVIPVLIHGDAAFAGQGVVMETLNLSQTRGYGTGGTV
                     HIIINNQIGFTTSDPRDSRSTLYCTDIAKMIEAPIFHVNGDDPEAVVMVTEIALDFRM
                     QFHKDVIIDLVCFRKQGHNEQDEPMVTQPSMYRIINKHPGTRKLYADKLLASNVIHET
                     EADEMVQAYRDAMDAGINPNKTIRYDYKSPHAVNWVPFLNSGKWNQPVVTAVPIDDLI
                     AVAERLTDIPTGFKLNPRIEKILTDRREMGKGRLPLDWGMAENLAYATLLKDNFPIRI
                     SGQDSGRGTFFHRHAVLHDQNREQEQWENGTYIPLRHITPTQPDFTVIDSILSEEAVL
                     GFEYGYASAQPNELVIWEAQFGDFANGAQVVIDQFITSGEAKWGRLCGLVMLLPHGYE
                     GQGPEHSSARLERYLQLCAEYNIQVCIPSTPAQIFHLIRRQMIRPMRKPLIVMMPKSM
                     LRNKESVSLLEDLAMGHFQPVIPETENLSPDQVRRIILCSGKIYYELLAYRRKKAITD
                     MVLIRLEQLYPFPHEDFQQEIDRYGQAKEIIWCQEEPGNQGAWHRIQHYIIRHKRPDQ
                     KLGYALRPSSASPSVGYLALDRFRQNELIEAAFRDNL"
     gene            complement(3669..4970)
                     /gene="gltA"
                     /locus_tag="PKZ49_09845"
     CDS             complement(3669..4970)
                     /gene="gltA"
                     /locus_tag="PKZ49_09845"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HND36906.1"
                     /translation="MTEQRKATLRLGENTEPIEFPILSGNMGPDTVDIRTLYAKTGLF
                     TYDPGFLSTASTKSSITFIDGDKGILLYRGYPIEQLAARCDFMEVCHLLLTGELPTDP
                     QKQHFVSTIKSHTMLHEQLVRFYTGFRRDAHPMAVMVGVVGALSAFYHEAMDISDVLH
                     REQSAFRLIAKLPTIAAMAYKYNIGQPFIYPRNDLSYAANFLHMMFATPAEKYEPNPV
                     IVRALDRILILHADHEQNASTSTVRLAGSSGANPFACISAGIACLWGPAHGGANEACL
                     NMLEQIGDVSRINEYIKRAKDKTDGFRLMGFGHRVYKNFDPRAKLMRETCHEVLNEME
                     LHNNRLFKLALELERIALEDDYFIAKKLYPNVDFYSGIVQKALGIPTSMFTAIFAMAR
                     TVGWVAQWNEMIADPDQKIGRPRQLYTGTGLRDIPADKINN"
     gene            complement(5058..5294)
                     /locus_tag="PKZ49_09850"
     CDS             complement(5058..5294)
                     /locus_tag="PKZ49_09850"
                     /inference="COORDINATES: protein motif:HMM:NF015870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HND36907.1"
                     /translation="MTKRIERIRWRCRRGLLELDLLLQRFLNKYDSQLNEQQIDQFES
                     LLSLSDNDLLAIICSNPETVDKSLKRLIQLIQRS"
     gene            complement(5291..5986)
                     /locus_tag="PKZ49_09855"
     CDS             complement(5291..5986)
                     /locus_tag="PKZ49_09855"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HND36908.1"
                     /translation="MRFLISRFDPDKDEKPYMQDYEIELEPTDKMLLDALIRIKSIDD
                     SLSLRRSCREGVCGSDAMNINGRNGLACITPIATLKEPIEIRPLPGLPIIRDLIVDMS
                     QFFKQYHSIKPYLVNHDPPPETERLQSPSERAELDGVYECILCACCSTSCPSFWWNPD
                     KFVGPAGLLQAYRFLADSRDQATTERLDYLEDPYRLFRCHSIMNCVDVCPKGLNPTNA
                     IGKIKGMMLNKVV"
     gene            complement(6105..>6390)
                     /locus_tag="PKZ49_09860"
     CDS             complement(6105..>6390)
                     /locus_tag="PKZ49_09860"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112843.1"
                     /note="catalyzes the formation of L-threonine from
                     O-phospho-L-homoserine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HND36909.1"
                     /translation="ETIRETYKKYGVLVDTHTADGIKVGLENRESDIPLICLETALPV
                     KFSDSIQEAIGKEPEPIPGFEDLEKLPQRFIVMDKDVNEVKQFISSKVRS"
BASE COUNT         1733 a         1290 c         1385 g         1982 t
ORIGIN      
        1 catattgact tcattaaaag tggtcagaat tgctgctgtc gattgagatt gcacaagtcg
       61 ttccgcaatg cgcattctga gacgggtcat tgggacacgt tgctcaagtc tttcagtgtt
      121 actcgctaat tgctccatat taggtacttc agaaagtatg ctggcagggg tggaagtaac
      181 ctcagcggtc tgcggtttgc ttttaatgtg attcgcgata tcttctttgg tgacgcgacc
      241 ttctcggccg gttcctacga ttgagttgac ttcattttgc tttaactgat tttcctccgc
      301 cattttttgg gctgctggca tgagtatttt ggctatttga tttttagttt ctggtttagg
      361 tttcacggtg ccttgattta tcgtcaaact tgagctaact gaggctgtgg aagtcatctc
      421 tgtttcaatc ttggctatga tttccccact tttgacggtt gctccatcat tttttagtat
      481 ttcaattaag atgccatcct gcgttgcggg tagctcaagt acaactttat cggtttcaat
      541 atcgattaaa ttctcaccac gaactacttt ttcaccttgc ttcttatgcc aagaaatcaa
      601 agtggcttca gcgactgatt cggataattg aggaactttg atttcaataa gcatattatt
      661 ttccttatta aaggttgtct ctgaaagctg cttcgatgag ttcattttgc ctgaatcgat
      721 ccaatgccaa atatcctacg gatggtgaag ctgaggaagg tcgtaacgcg tagccaagtt
      781 tttgatcagg acgtttatgt cgaataatat aatgttgaat tcgatgccaa gcgccttgat
      841 ttccaggttc ttcttgacac caaatgattt cctttgcttg tccatagcga tcaatttctt
      901 gttgaaaatc ttcatgagga aaaggataaa gttgttcaag acgaatgagc accatatccg
      961 tgattgcttt ttttcttcga tatgcgagta gctcataata aatttttcca ctacacaata
     1021 tgatccgtct aacctgatca ggtgacagat tttccgtttc tggaataacg ggttgaaaat
     1081 gacccatagc aagatcctcg agtaaagaaa cggattcttt atttcttagc atactcttgg
     1141 gcatcatgac gattaatggc ttgcgcatgg ggcgtatcat ttgtctgcga atcaaatgaa
     1201 atatttgagc tggcgtagac ggaatgcaaa cttgaatatt atactcagcg cagagttgca
     1261 gataacgttc caatcgtgct gaggaatgct cagggccttg accctcatag ccatggggaa
     1321 gcagcattac caaaccacat aatcgtcccc atttagcttc tcccgaagtg atgaattgat
     1381 caatgactac ctgagcacca tttgcaaagt caccaaattg agcctcccaa atgactaatt
     1441 cattgggttg ggcagaggca tagccgtact caaaccctaa aacggcttct tccgaaagta
     1501 tagaatcgat cacggtgaaa tcaggttgcg tgggcgtaat atgtcttagt ggaatgtaag
     1561 taccattttc ccactgctct tgttctcgat tttgatcgtg taatacggca tgccgatgaa
     1621 agaaggtgcc acggccactg tcctgaccag aaatccgaat tggaaagtta tctttcaaaa
     1681 gtgttgcata ggctaaattt tcagccattc cccaatcaag cggtagtctc cctttcccca
     1741 tctcgcgtcg atcagtcaaa attttttcaa tccgtgggtt taatttgaaa ccagtgggaa
     1801 tgtctgtgag tctctcagct acggctatta aatcatcaat tggtacagcg gttaccactg
     1861 gttgattcca tttgcctgaa ttaaggaaag gcacccagtt aaccgcgtga ggtgatttat
     1921 aatcatagcg gatcgttttg tttggattaa tgccagcatc cattgcgtcc cggtatgcct
     1981 gaaccatctc atccgcttca gtttcatgga tgacattgga tgcgagcagc ttgtctgcat
     2041 atagttttct tgtgccaggg tgtttgttaa taatccggta cattgaaggt tgtgtcacca
     2101 tcggttcatc ttgttcattg tggccttgtt tgcggaagca aaccagatca atgatgacgt
     2161 ctttgtgaaa ttgcatgcgg aaatctaaag caatctcagt gaccataacg acagcttcag
     2221 ggtcatcgcc attcacatga aaaattgggg cctcaatcat tttggcgata tcagtacagt
     2281 agagcgtcga gcggctatcc cgtggatcgg atgttgtaaa accaatttga ttgttgataa
     2341 tgatatgaac tgttccacct gtaccatagc cacgagtttg cgacaaatta agtgtttcca
     2401 tcacaacgcc ctgtccagca aaagcagcat caccgtggat caatacggga atgacgagtt
     2461 gtccttgttt gtcactgagt cgatgttggc gtgcccgaac ggatccttca actacaggat
     2521 caacgatttc gaggtgggaa ggattgaaag ctagcgctag atgtacgata ccacctggag
     2581 tcatgacggc agaggagaag ccttgatggt atttaacatc acctgccatt agtgtttgga
     2641 catgcttgcc ttcaaattcg agaaataaat ctgagggcat ttttcccaat gtattaacca
     2701 gtacgttcag tcgaccacga tgcgccatac ctaaaacaat ttcttgaact cccatcaatc
     2761 ccgcatgctg gataagttga tctaataatg ggataaggct ttcattacct tcacctgaaa
     2821 agcgcttctg gccgacatag cgagtatgca ggtatttttc taatccttcc gcagcagtta
     2881 aacgttctaa gatatgcttc ttatactcat tcccataact cggttgtgcg caggtgcttt
     2941 ctaggcgact ttgaatccaa cgcttttgtt ttaaatcggt aatgaacata tattcagcgc
     3001 cgatcgcatt gcaataagtt tgctgaagaa tttgtaatat ggttcgaagt ggcgcatgtt
     3061 ccgggccgac tagcgacccg gtgttaaagt ttgtgtctag atcattcgct gttaatccat
     3121 gaaaagcagg gtcaagttct ggaacatcga gtagcggctg atggttgagt gggtcaagtt
     3181 tggcacatct tacacctagg aatcgataca tattgatcaa ctgcaacact gcaatttgct
     3241 tacgttcttt ggtttcatcg taaggctggt tgaatagtgg ttgtgtaagg cttttgtctt
     3301 gaattgaggc tatcttatta actgggttga ctaatgattg aatgatcaag gagtggggtc
     3361 gatcttttgt gctgattgca atggtttgtt gaagattatc aaagaagctg cgccattctg
     3421 gagagataga ctcgggacta cttaagtagc gctcatagag atcctcaaca aaaggcgcat
     3481 tggtaccaaa cagaaatgaa ttttcgataa attgtctcat catgcaccaa cccatttaat
     3541 ccagctgaaa atatttcaca ataaagtgaa gttattttca aactatgttg aaaaccgatt
     3601 tataaaaata acagcatgtt tggcatgctg cttcaaatta attttgaggc attcttatcg
     3661 tataaaaatt agttgtttat tttatcagcg gggatatcgc gaagcccggt acccgtatag
     3721 agttgtcgag gtcgacctat tttctgatct ggatccgcaa tcatttcgtt ccattgtgct
     3781 acccaaccga ccgttcgtgc cattgcaaag atagcggtaa acatcgatgt tgggataccg
     3841 agtgcttttt gcacgatgcc agaataaaaa tcaacattgg ggtaaagttt cttcgcgata
     3901 aagtagtcat cttcaagtgc aattctttct agttctaaag caagtttgaa aaggcggttg
     3961 ttatgtagtt ccatttcatt taatacttca tggcacgttt cacgcattaa ttttgcacga
     4021 ggatcaaaat ttttatagac gcgatgaccg aaccccatta atcggaatcc gtccgtttta
     4081 tcttttgcac gtttaatata ttcattgatg cgtgaaacat ccccgatctg ttccagcata
     4141 tttaagcagg cctcattggc gccaccgtga gcgggtcccc ataagcaggc gattcctgct
     4201 gatatacacg cgaatggatt ggcaccactt gatccagcga gccggaccgt tgaggtcgat
     4261 gcattttgct catgatcggc atggagaatc agtattcgat ctagtgctct gacaataacc
     4321 ggattcggtt catatttttc tgctggtgtc gcaaacatca tgtgcaaaaa gtttgctgca
     4381 tagcttaagt cgttgcgtgg gtagataaaa ggttggccaa tattgtattt gtaggccatt
     4441 gcagcaatag tgggcagctt ggcgattaaa cggaaggctg attgctcacg atgtagcacg
     4501 tctgaaatat ccattgcctc atggtaaaaa gcagataaag cgcctacgac acctaccata
     4561 acagccatgg gatgagcatc tcttcggaat cctgtgtaga atcgaacgag ttgctcatgc
     4621 aacatggtgt gagattttat ggtgctcaca aaatgttgtt tctgaggatc agtaggtaac
     4681 tcgccagtca acagtaggtg acatacttcc ataaaatcac agcgtgccgc aagttgctca
     4741 atcggataac ctcgatagag cagtattcct ttatcaccat cgataaaggt aatacttgat
     4801 ttggtgcttg cggtcgatag aaagccagga tcataagtga ataggcctgt tttggcatac
     4861 agtgttcgta tatcaaccgt atcagggccc atattgccgg ataaaatcgg aaactcgata
     4921 ggttcagtgt tctcgccaag tcgtaaggtt gctttacgtt gttcagtcat atttattttc
     4981 tccttctctt tgggttgaga aacgatctcg ttattgattt attattaact attctgcact
     5041 tttacagatt caagaattta acttcgttga atgagctgta taagtcgttt taagctttta
     5101 tcaaccgttt caggatttga acaaattata gctaacagat cgttatctga aagtgacaac
     5161 agtgattcaa attggtctat ttgttgttca tttaattgag aatcgtattt attcagaaat
     5221 cgttgtaaga gtagatcaag ctcgagcaaa cctcttcgac agcgccagcg tattcgctcg
     5281 atacgcttgg tcatactact ttattgagca tcatgccttt gatttttcct atcgcattgg
     5341 ttggattgag tcctttgggg cagacatcaa cacaattcat aatggaatga cagcgaaaca
     5401 ggcggtatgg atcttcaaga taatcaagtc gttcagtggt ggcttgatcc cgactatcag
     5461 caagaaagcg ataagcttgt aagagaccgg caggtcctac aaatttatcg ggattccacc
     5521 aaaaagaagg gcatgaagtt gaacagcagg cgcataaaat acattcatat acaccatcta
     5581 attctgctct ttcgcttggt gactgtaatc gttcagtctc ggggggagga tcatggttaa
     5641 cgagataagg ttttatcgaa tgatactgtt taaagaattg ggacatatcg acgatcaggt
     5701 cacgaataat cggcagaccg ggtaatggac gaatctcgat cggttctttg agggtagcga
     5761 tgggtgtaat gcaagcaagt ccattacgtc cattgatatt catggcatct gaaccacata
     5821 cgccttctcg acaggatcgg cgtaagctca ggctatcatc aatagatttg atgcgaatta
     5881 acgcatctaa aagcatttta tctgtgggtt caagttcaat ttcataatcc tgcatatagg
     5941 gtttttcatc tttatcagga tcaaaacgac taattaagaa tcgcatcatt gactaccttt
     6001 ttagctaaaa cgttaaacta attattcagc atatcagatt atgataaatt taaaatgaaa
     6061 gaaagcgaag atatcggttt tatggaatga atttaaccgt tttattatga tcgtacctta
     6121 cttgaaataa actgctttac ttcattcaca tccttatcca ttacgatgaa tcgttgtggg
     6181 agtttttcaa gatcttcgaa gccaggtatc ggctcaggtt ctttcccgat tgcttcttga
     6241 atactatctg agaatttaac aggtaaagca gtttccaaac atatcagcgg tatatctgat
     6301 tcgcgatttt ctagcccaac tttaataccg tctgcagtat gagtatcaac taatacacca
     6361 tattttttat aagtttctcg aatggtttcg
//
LOCUS       DAOQKQ010000155         6373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_6192_length_6373_cov_4.939221, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000155 DAOQKQ010000000
VERSION     DAOQKQ010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 6373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6373
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6192_length_6373_cov_4.939221"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /gene="sucB"
                     /locus_tag="PKZ49_09865"
     CDS             <1..758
                     /gene="sucB"
                     /locus_tag="PKZ49_09865"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006554 - lysine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HND36910.1"
                     /translation="PANALSEVPNVEQLANNTERLEQRVPMTRLRMRIAERLVQSQST
                     AAILTTFNEVNMQAVIDLRTRYKDKFEKEHGVKLGFMSFFVKAVVAALKKFPIINASV
                     DGNDIIYHPYYDIGIAVASPRGLVVPILRNADQLTQVEIEKQIADFVTRAQNGKLTID
                     ELSGGTFSITNGGVFGSMLSTPIINPPQSAILGIHATKERPVVENGQIVIRPMNYLAL
                     SYDHRIIDGKEAVLSLVTIKEALEYPMSPLFES"
     gene            complement(780..4286)
                     /gene="dnaE"
                     /locus_tag="PKZ49_09870"
     CDS             complement(780..4286)
                     /gene="dnaE"
                     /locus_tag="PKZ49_09870"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HND36911.1"
                     /translation="MPIDPAFIHLRLHSEYSITDSIIRIDEVLARAAQDHMPALALTD
                     LGNLFGLVKFYQSAEKIGIKPIIGCDAWISNEADRNNPFRVLLLCQTHSGYLLLCRLL
                     SRAYRENLFRGRAELKKAWLHSSAEGTEDLIAISGAHFGEVGQLIIQNNLQEAQVVSK
                     EWSELFPNRFYLEIQRNGRVDEERFIRNSLILAAQLNIPIVATHAIQFLDAKDYKAHE
                     ARVCIAEGYVLGDRRRPRAFTDQQYFKTQSEMQALFADIPEALANSVEIAKRCNLQLE
                     LGINRLPLFPVPDTTSIEAYLSNQALSGLKQRLQDLPENEFNHKLTTYQARLNFEVNT
                     IIQMGFAGYFLIVADFINWAKQNNIPVGPGRGSGAGSLVAYSLGITDLDPLRYDLLFE
                     RFLNPERISMPDFDIDFCQDRRESVIEYVKNRYGMESVSQIVTFGTMAAKAVIRDVGR
                     VLDLPFNFVDQLAKLIPFELGMTLKKARAIEPQFESRAREEEDVRNLLELAESLEGIT
                     RNVGMHAGGVLIAPGKITDFCPIYCAEASESVVSQFDKDDVEKIGLVKFDFLGLKTLT
                     ILDQAISDINQQQLHDIKYQTEPESTGGISLSQIALEDPETYALLRKGNAVGIFQFES
                     RGMKDLLQKAKPDRFEDLIALVALYRPGPMDLIPDFIERKHGIQRVEYLDPRLEPILG
                     PTYGVMIYQEQVMQIAQVIGGYSLGGADLLRRAMGKKKVEEMAQQRDIFVEGAIKNGL
                     TEQNAIKLFDLMEKFAGYGFNKSHAAAYALIAFQTAYLKAHYPAAFLAACLSAEMNDT
                     DKINDFYEDCKVNGINVLPPDINFSMYRFVPTDAKTIRYGLGAVKGTGENAITAIVQA
                     RERSGLFTDLFDFCKRVDRRLVNRRSIESLIRVGAFDGIQSNRAMLFASVGSAMEFAE
                     QSSLSTQQVNLFSETEVNAQLPTLLEHVAWSEKEKLQNEKLGLGFYFSGHPFSFYLKE
                     LNQFITTRLDKLRSQREPQLIAGIIHAIRTQMTKRGKMAIVTLDDGRARVDIVIYNEI
                     LGEHSSWLKEDQLLVAKVIIGNSMNDPNELRITAEKLYDLTNARSQSAKKIQFTINQS
                     IIDCKLVVPKLKELIAASLPVAKGNHGQVQPTRCPVTILYQNQQATCVIELGDAWHVL
                     LSDFFIESVSQLFKPENVEILY"
     gene            4346..5698
                     /gene="yegQ"
                     /locus_tag="PKZ49_09875"
     CDS             4346..5698
                     /gene="yegQ"
                     /locus_tag="PKZ49_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HND36912.1"
                     /translation="MQTLPELLLPAGSLEKMRTAYAFGADAIYAGQPRYSLRARNNEF
                     SLEQLRTGIIEAHQLNKKFFVASNLMPHNRKVKTYMADMEPVIDLKPDALIMADPGLI
                     MMVREKWPEIPIHLSVQANTVNYAAVKFWQKIGLTRIILSRELSLEEIAEIRQLCPDI
                     ELEVFVHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVKPAQENPEGDIQAL
                     QTIDFDFNQAVSQSALDDASPIRHPAADHVYLIEEKERPGQLMPILEDEHGTYIMNSK
                     DLRAIEHIEQLIHIGIDSFKIEGRTKSAYYVARTAQIYRKAIDDAVAERPFDYTLLGQ
                     LENLANRGYTAGFYQRHASQETQNYLRGHSESNQSQYVGDITTSDESNGMVEILVKNR
                     FGIGDRLEIIHPSGNRIIELDYMQDHNGKTIDVAPGSGHRVRIPLTGNVEKAFIAKLL
                     "
     gene            complement(5797..>6373)
                     /locus_tag="PKZ49_09880"
     CDS             complement(5797..>6373)
                     /locus_tag="PKZ49_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36913.1"
                     /translation="KISLLSDAGAGVFTNNFLNTVFEPNGPWGTENTLATWIPGINQI
                     SSYNELNFFTNLTTGIERYFDKSKFAYVTTAWDGVQVLFLNIMKKTDQGINDPIQWFN
                     LTPEVFVEWNSRMLASFSVNVLFNSNAKYYVSAGTYHAGLVDVFVPDIFYTENSASGI
                     FLKDWVNRLVINDKKYPLINLMCSGTCGAPF"
BASE COUNT         1902 a         1461 c         1183 g         1827 t
ORIGIN      
        1 ctcctgccaa cgcgctttct gaagtaccga atgtggagca attagcgaat aacactgaac
       61 gacttgagca acgtgtccca atgacccggc tcagaatgcg cattgcggaa cgacttgtgc
      121 aatctcaatc cacagcggca attttaacta catttaatga agtcaatatg caagccgtga
      181 ttgatctacg cacacgatat aaggataagt ttgagaaaga acatggggtc aaattaggtt
      241 tcatgtcgtt ctttgttaaa gcagttgtcg ctgcactaaa gaaattcccg atcatcaatg
      301 cttcagtcga tggaaatgac atcatctatc atccctatta cgatatcgga attgctgttg
      361 ccagcccacg gggcctggtt gtgccaattc ttagaaatgc agatcaacta acacaagtag
      421 aaattgaaaa gcaaattgcc gattttgtta ctcgcgctca aaacggtaaa ctcaccattg
      481 atgaattatc cggcggaaca ttttccatta ctaatggtgg tgtttttgga tcaatgctgt
      541 ctaccccgat cattaatcct ccccaaagcg ctattttagg cattcatgct accaaagaac
      601 gtcccgttgt tgaaaacggt caaattgtaa ttagaccgat gaattatctt gcgctttcct
      661 acgatcaccg cattattgat ggtaaagaag ccgttctctc gttagttaca atcaaagaag
      721 cattagagta tccgatgagt cctttattcg agtcttaaac ttagattcat cgaacttgct
      781 cagtatagaa tctcgacatt ttcgggttta aataactgag aaaccgattc aataaaaaaa
      841 tcacttaata aaacgtgcca tgcatcaccc agctcaatca cacacgttgc ctgctgattt
      901 tgataaagaa ttgtaacggg gcatcgtgtg ggttgtactt gcccatgatt gcctttagca
      961 acgggtaaac tcgcagcaat caattccttt aacttaggca caaccaattt acaatcaatg
     1021 attgactgat taatcgtaaa ttgaatcttc ttcgcggatt gagaacgtgc attggtaagg
     1081 tcatataatt tctccgccgt gattcgcagt tcattgggat cattcatact gttaccgatg
     1141 attaccttcg caactaataa ttgatcttcc tttaaccaac tactatgttc gcctaagatt
     1201 tcgttataaa tcacgatatc gacccgtgcc cggccgtcat cgagtgtaac gattgccatt
     1261 ttccctcgct tggtcatctg cgttcggatc gcatggatta ttccggcaat caattgtggc
     1321 tcacgctgtg atcgaagttt atctaaacgc gtcgtgataa attgattcaa ttcttttaga
     1381 taaaacgaaa agggatgacc actaaaataa aacccaagac ctagtttttc attttgtaac
     1441 ttttcttttt cagaccatgc cacatgctca agtaaggtag gtagctgagc attaacttca
     1501 gtttcgctga atagattgac ttgctgagtt gataaactcg attgttcagc aaattccatt
     1561 gcgctgccaa ctgacgcaaa taacatagca cgattggact ggataccatc gaaagcaccg
     1621 actcgaatta aggattcgat gctacgccga ttcacaagcc ggcggtctac gcgcttacaa
     1681 aaatcaaaga gatcagtaaa caaaccactc cgttcacgcg cttgtacaat agctgtaatc
     1741 gcgttttccc ctgtgccttt aacagcgcct aatccatagc gaatcgtttt tgcatcggta
     1801 ggtacaaacc gatacatcga gaaattgata tctggcggta aaacgttaat tccatttact
     1861 ttgcaatcct cataaaaatc attaatcttg tctgtatcat tcatttccgc agacaaacag
     1921 gcagctagaa aagccgctgg ataatgcgct ttgagataag cggtctggaa cgcaattaat
     1981 gcataagccg ccgcatggga cttattgaat ccatatcccg caaatttctc catgagatcg
     2041 aatagcttaa tggcgttctg ttcagtcaat ccatttttga ttgcaccttc aacaaaaata
     2101 tcacgctgct gagccatttc ctcaactttt ttcttaccca tggcgcgacg caataaatcg
     2161 gctccaccca aactataacc accaatgact tgcgcaatct gcatcacttg ttcttgataa
     2221 atcatcacac cataggttgg tcctaatatg ggctcaagtc ttggatccaa atactcgaca
     2281 cgttgaatcc catgcttacg ctctatgaaa tcaggaatca aatccatagg acctggccta
     2341 taaagcgcaa ctaaggcaat tagatcttca aatcgatcgg gtttggcttt ttgtaataaa
     2401 tctttcatgc ctcgcgattc aaattgaaaa atccccaccg cattgccttt tcttaataaa
     2461 gcataggttt caggatcttc caaagcaatt tgactcaaag agatgccgcc ggttgattca
     2521 ggctcggttt gatatttaat atcatggagc tgttgctgat tgatgtcact gattgcttga
     2581 tcaagaatcg ttagtgtttt taaacccaga aaatcaaact taacgagtcc aattttctca
     2641 acatcgtctt tatcaaactg actgaccaca gattcactcg cttccgcaca ataaattgga
     2701 cagaaatctg taatttttcc gggcgcaatc agcacgccac cagcgtgcat acccacattt
     2761 cgggtaatac cttcgagact ttcagctaac tctaataaat tgcgcacatc ctcttcttct
     2821 cgtgcacgcg actcaaactg aggctcaata gcacgcgctt tctttagtgt catccctaat
     2881 tcgaatggaa ttaatttcgc aagttgatca acaaaattga atggtaaatc taaaacacgc
     2941 ccaacatccc gtatcactgc tttggcagcc atcgtcccaa atgtcacgat ttgagagacg
     3001 ctttccatac catatcggtt ctttacatac tcgatgacac tttcacgacg gtcttggcaa
     3061 aaatcaatat caaaatcagg catcgagata cgttctggat ttagaaaacg ttcaaacaac
     3121 aaatcatatc gtagcggatc gagatcggta attcctaacg aataagctac taaagaacca
     3181 gctccagatc ctcgaccagg accaacggga atgttgtttt gcttagccca attaataaaa
     3241 tccgcaacaa tgagaaaata gccggcaaac cccatctgga taatggtgtt cacttcaaaa
     3301 ttgagcctag cttgataagt tgttaatttg tggttgaatt cattttccgg taaatcttgc
     3361 aaacgctgct ttaaacctga taatgcttgg ttgcttaaat atgcttcaat gctggtcgtg
     3421 tcaggtactg gaaataacgg taatcgatta atacccagtt caagctgcaa attacatcgt
     3481 ttcgcaattt cgacactatt ggctagcgct tccggaatat ccgcaaacaa agcttgcatt
     3541 tcactttgtg ttttaaaata ctgctgatca gtaaatgcac ggggacgccg ccgatctcct
     3601 agtacataac cttctgcaat acaaactcta gcttcatgtg ctttataatc ttttgcatcc
     3661 aaaaattgaa tggcatgcgt ggccacaatc ggaatattta attgggcagc gagtatcagc
     3721 gagtttcgaa taaaccgttc ttcatccacc cgcccatttc gttgaatctc cagataaaat
     3781 cgattcggaa ataattcaga ccattcttta ctgactactt gggcttcctg cagattattt
     3841 tgtattatca attgacccac ttccccaaaa tgtgcgccgg atattgcaat taaatcttca
     3901 gttccttctg cagaagaatg aagccatgct ttcttcagtt ctgccctgcc ccggaataaa
     3961 ttttcacgat aggcacgcga caacaatcgg cacaataata gatagcccga gtgggtctgg
     4021 catagcaata gtacgcgaaa ggggttattt cgatcagctt cattactaat ccaagcatca
     4081 cacccaatga tgggctttat ccctattttc tctgcgcttt ggtaaaattt aacgagtcca
     4141 aataaattac ctaaatcggt aagcgctagg gctggcatat gatcttgagc tgctcttgct
     4201 aatacttcat caatccgaat aatactgtcc gttatcgaat attcgctgtg cagtcgtaaa
     4261 tgaataaaag caggatctat aggcatagtg ctacaattta catcaattaa ttaattatcc
     4321 aagccaatat tggtcatttt acatcatgca aactttgcca gagctattac tcccagcagg
     4381 ttctttagag aaaatgcgga ccgcctatgc ttttggggct gatgcaattt atgcaggtca
     4441 gccacgctac tcgttacggg ctcgcaataa tgaattttca cttgaacaac tacgcactgg
     4501 aattattgag gcacatcaac ttaacaagaa attttttgtc gcgagcaatc tcatgcccca
     4561 taaccgtaaa gtcaaaacct atatggcaga tatggagcct gtcatcgatt tgaaaccgga
     4621 tgccctgatc atggctgatc caggtctaat catgatggta cgagagaaat ggccggaaat
     4681 tcccattcac ctctcagttc aagccaatac ggtaaattat gctgccgtta agttctggca
     4741 aaaaattggc ctcactcgta ttatcctatc acgtgaactc tctctggaag aaatagctga
     4801 aatccggcag ctatgtcctg acattgaact cgaagttttc gtccatggcg cattatgcat
     4861 cgcctactca ggaagatgtt tgttatctgg gtattttaac caccgcgacc ccaaccaagg
     4921 tacctgcacc aactcctgcc gttgggacta taaagtcaaa ccggctcaag aaaatccgga
     4981 gggtgatatt caagcgctgc aaacgattga cttcgacttt aatcaagcag tttctcagtc
     5041 tgcattggat gacgcttcgc caattcgcca ccctgcagcc gatcatgttt atttgattga
     5101 agaaaaagaa aggcctggtc aattgatgcc cattctagaa gatgagcatg gaacttatat
     5161 catgaactca aaagacctac gcgcgattga acatattgag caactcattc atattggaat
     5221 tgactcattc aaaattgagg gccgtactaa atcagcctat tacgttgcac gaaccgcaca
     5281 aatatatcga aaagccattg atgatgctgt cgcagaacgt ccgtttgatt acaccttgct
     5341 tggtcaactc gaaaacctgg cgaatcgcgg gtatacagct ggtttttatc agcgccacgc
     5401 aagtcaagaa actcagaatt acctacgcgg tcactccgaa tccaatcaaa gccaatatgt
     5461 tggagatatc actacttcag atgaatccaa cggaatggtc gaaattcttg ttaaaaaccg
     5521 ttttggtatc ggtgatcggc tggaaatcat tcacccatcg gggaatcgta ttattgagct
     5581 cgactatatg caagatcata acggaaaaac tattgatgtt gcaccaggga gcggccatcg
     5641 cgttcgtatc ccattaaccg gaaatgttga aaaagccttt attgcaaaat tactctgata
     5701 ctactactcc agtagtttta aattttgtgt agtctctggt aacaaatacc agagactaca
     5761 cctatacctc tctcagaaag tgattacttt ctttccttaa aaaggcgctc cacaggtacc
     5821 actacacatc aaattaatca aaggatactt tttatcattg atgactaacc gattaaccca
     5881 atcttttaag aaaataccac tcgcactatt ttcagtgtaa aagatatcgg gtacgaacac
     5941 atcaaccaga ccagcatgat aggttccagc acttacgtaa tacttagcgt tgctattgaa
     6001 taatacattc acgctaaatg aagccaacat tctcgaattc cattcaacaa agacttcagg
     6061 tgtcaaattg aaccattgaa ttggatcatt aattccctga tcggtctttt tcataatatt
     6121 gagaaatagc acttgaacgc catcccacgc agttgtaacg taggcaaatt tacttttgtc
     6181 aaaataacgt tcgatacccg ttgtcaaatt agtaaaaaaa tttagctcgt tatagctact
     6241 aatctgattg attcctggta tccaagtcgc taaggtattc tctgttcccc atgggccgtt
     6301 cggctcaaaa acggtattca aaaaattatt ggtaaataca cccgcacctg cgtcagataa
     6361 taatgaaatt ttt
//
LOCUS       DAOQKQ010000156         6190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_6448_length_6190_cov_5.556316, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000156 DAOQKQ010000000
VERSION     DAOQKQ010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 6190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6190
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6448_length_6190_cov_5.556316"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="PKZ49_09885"
     CDS             <1..770
                     /locus_tag="PKZ49_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HND36914.1"
                     /translation="QKWLLKLPRVHAVDRLLLQSGMAIMVMPFLGYSLLTGLGGMALA
                     AILGFSFAVILLSMVVGCLIPYFFVLRARHKRLITIEQQLPEIIDLMSRALKAGHAFT
                     GALQMAATEGTEPAASEFRLVFDEINFGVPIQNALMNLANRVPITDLSYFVIAVLIQR
                     ESGGNLAELLDKISNLIRSRLNLLGRIRVLSAEGRLSAWILSCIPFVMALILNILNPG
                     FMSILWTDPVGPTIIAIALVMMVVGIYAMSRIIKIHV"
     gene            854..1801
                     /locus_tag="PKZ49_09890"
     CDS             854..1801
                     /locus_tag="PKZ49_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HND36915.1"
                     /translation="MISMQTIFLVMIFLAVAGISCAIMIILSPPATKARLKEISSHDD
                     ALLDGYKAADEWKETVVKLSEPLAKLALPSEEWEQSALRTRFMHAGYRGNAALTIYFA
                     TKTFLALLFPGLFFFFAGIGQLQLTSVNLTLILLIFTAIGFYLPNLLLARKIKLRQRE
                     LFESFPDAIDLMTVCVEAGLGLDAALAKVGEEMELRSPILGEEIHLVGLELRAGASRE
                     KALRNLALRTGVEEIEALVAMLVQADRFGTSTADSLRVYSDTLRTKRRLRAEEAAAKI
                     ALKLLFPLIFFIFPALMVVLMGPAFISIYRNLLPALAGQ"
     gene            1813..2313
                     /locus_tag="PKZ49_09895"
     CDS             1813..2313
                     /locus_tag="PKZ49_09895"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HND36916.1"
                     /translation="MLHCLRQLLILAGCFLLLVGCATQSTNSLLTQNDVPSVKKQMGP
                     DLVKLYLQGKKYQENANYSEAISAYEAILAKDPDFVEAHNGLGVIYSAQEKFELALQH
                     LQTAVKLAPLATHLHNNLGYTYFLLGQDEKAIKAFEQSLYLDPENERAQFNLSLAHQK
                     ISKSGE"
     gene            2316..3368
                     /locus_tag="PKZ49_09900"
     CDS             2316..3368
                     /locus_tag="PKZ49_09900"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HND36917.1"
                     /translation="MRFQKRKIVLAIGCISALTGCASYFSGKEFSAYEVRPVTNIQQA
                     ESSAENFYHIGEYYQRKKQWLNALESYQKALSLQTEYVEAHNGLGIVYATLGQYELAI
                     DHFKKAITLDASKSYLHNNLGYAYYLQGVYHEAELSFQAAISNDPRNGKAKKNLTLVH
                     KKMGVSEKLARTLKATSNSETMSTKYPAQLTHENHLSQQLVQVAPNVYEFRSQRETQN
                     KVENKLVSIVMHDEQETQLQKNIKSLAQGIEVSNGNGVTGMARNVATQLNNAGLGQAR
                     LTNHQTFKQVQTEIYHRVSAHALAEKITQLLPGQIKLIESDDLREDINLKILLGQDIA
                     NFKALSHNSHQVTKVM"
     gene            complement(3392..4255)
                     /locus_tag="PKZ49_09905"
     CDS             complement(3392..4255)
                     /locus_tag="PKZ49_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HND36918.1"
                     /translation="MPAPNKIKQSLREIDSLPAMPVIAQKMLALPLDTTEGEAQLLKL
                     ITQDPQISARIVGLSNSSFFSMPSTITSVSDAAMLLGLTQVKSIAIGMATITVLAKLP
                     EGKLKSMDLWAHSMAIATTMRTIARHMPANLRPIDDQIFLAGLLHDIGYSTLSYLDTE
                     TSNRLHEQLNNTANADLLAIEQEIVGIHHGEIGAQLGIHWGLPEELIAVMRYHHTPDH
                     PDATLGLPIIRLVNTAEKLLSNFWINEHTAQEITKQEWSNLGISENKIDSISEEVKTL
                     AENASQLVSAA"
     gene            4410..5210
                     /locus_tag="PKZ49_09910"
     CDS             4410..5210
                     /locus_tag="PKZ49_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36919.1"
                     /translation="MFQNELLSSYPVNRIILVGASNLTLSLRMIIQHIQGSVGGPSEI
                     LVAAGHGRSYGLSSQVMIRELPGIVRCGLWEQLSTRVIIPSYALLTDIGNDIPYGVCP
                     EQILQWVTWCVDELTKASAQIVITNLPIESIEALSNRRFQFFRHLFFPSCTLSRIEVV
                     SRAKVVHQGIATLAQQKQLILCEQSRQWMGADGFHVAFMKREQVFRQLCNHFVGTDED
                     LSSVPKQSELRLSWQQRPRFALQRVWGKEKCCQQPSGLLSNQATVSLY"
     gene            complement(5233..6075)
                     /locus_tag="PKZ49_09915"
     CDS             complement(5233..6075)
                     /locus_tag="PKZ49_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36920.1"
                     /translation="MRIDQIIIKLIVLPILLVVHYAHASYLDQTTPNGQVRIHYSSTT
                     PASCDAILLGVGTSMSISSYDKLSEELIKYGYVTVIMDHAPGNMVKTDPQKYATLAND
                     VKTNLNSWLSGIGCNTVTHWLMGGHSAGGQAAQNAISNGLVQADAIFSIDPYNANNAG
                     NVNLPAMYWGFDVTTCFVDKNAAAKAAYLHSQNLRAFYRVAKQYSWGPCGYSPKYFHC
                     SFCDEHCPACTNCVLTPQSFFVDVANSVNKFITALFYGNWSKSALTISATTPLTLFVD
                     SELP"
BASE COUNT         1771 a         1165 c         1414 g         1840 t
ORIGIN      
        1 tacaaaaatg gttacttaaa ttgcctcggg tccatgcggt agatcgattg ctgcttcaat
       61 ccggtatggc aatcatggtc atgccatttt taggatatag cctgttgacc ggattgggag
      121 gaatggcttt agccgctata ttaggttttt catttgctgt cattttgtta agtatggtgg
      181 tcggctgctt aatcccttat ttttttgtgc taagagctag gcataaacgg ttgattacca
      241 tagagcagca attacccgaa ataatcgatt tgatgtctcg cgcgctcaaa gcaggtcatg
      301 cgtttactgg cgcattgcaa atggctgcaa ccgaaggaac ggaacctgct gcaagtgaat
      361 ttcggttggt attcgatgaa atcaattttg gtgtaccgat acaaaatgcg ctgatgaatc
      421 ttgctaaccg agtcccaatt accgatttaa gttattttgt cattgccgtg ctcattcaac
      481 gtgagagcgg tggtaattta gccgagttgc tcgataaaat tagtaacttg attcgttcaa
      541 gattgaatct attaggtcgc attcgagtgc tttcagcaga agggaggtta tctgcgtgga
      601 ttttgtcctg tatacccttt gtcatggcgt taattctcaa tattctcaac ccaggattta
      661 tgagcatctt atggactgat ccagttggac cgaccattat cgccattgcg ctagtaatga
      721 tggtggttgg tatttatgcg atgtcacgca tcattaaaat tcatgtttga cgtgttatta
      781 cgtttgatca gtcattgagg aacatcttga atttattgct gttgatcgat ccagttctag
      841 aggtatctaa gctatgattt ccatgcaaac tatttttctg gttatgattt ttctcgcagt
      901 ggcgggaatt agttgtgcaa tcatgattat tttatccccc cctgcgacca aagcgcgtct
      961 aaaggaaatt agcagccatg atgatgcatt attagatggc tataaagcgg ctgatgaatg
     1021 gaaagaaacc gtggttaaat tatcggaacc actggcaaaa ctcgctttgc ccagtgagga
     1081 atgggaacaa tcagcgcttc gcacgcgttt tatgcatgca ggctatcgtg gtaatgcagc
     1141 gctaacgatt tactttgcga ctaaaacgtt tttagctttg ctctttcctg gcctattctt
     1201 tttctttgca ggaattgggc agttacaact gacctcagtg aatttgacgt taatcttatt
     1261 gatttttacc gcgatcggat tctatttgcc taatttactt ctggcacgaa agattaaatt
     1321 acggcaacga gagcttttcg aaagcttccc cgatgcaatt gatttgatga cggtttgtgt
     1381 agaggcgggg ttaggattag atgctgcttt ggcgaaagtg ggcgaagaaa tggaattgag
     1441 aagtccgatt ctcggggaag agattcattt ggttggcctt gaattacggg caggcgcttc
     1501 ccgcgagaaa gcgctgcgta atctagcgtt aagaaccggc gtcgaggaaa tcgaagcatt
     1561 agttgccatg ttggtgcaag ccgatcgatt tggaactagt accgctgatt cgctgagagt
     1621 ctattccgat acattgcgaa ctaagcgccg tttgcgggca gaagaagcgg ctgcaaaaat
     1681 tgcactgaaa ttattattcc cgcttatttt ctttattttc cccgcgttga tggttgtgct
     1741 gatgggtcca gcatttatca gtatttatcg aaacttactt ccagcgttgg ctggacaatg
     1801 aggagttcta tcatgctgca ttgcttaaga caattgttaa ttttggccgg ctgtttttta
     1861 ttattggtag gatgtgcgac acaatcaact aacagtctgc tgacacaaaa tgatgtgcca
     1921 agtgtaaaaa agcaaatggg gcccgatttg gtgaaactct atttgcaggg caagaaatat
     1981 caagaaaatg caaactattc agaagccatt tcggcttatg aggctatttt agcgaaagac
     2041 cctgattttg ttgaagcgca taatggattg ggtgtgattt attcagcgca agaaaagttt
     2101 gagcttgcgt tacagcattt gcaaacggcg gttaagctgg cgcccctagc aacgcatctt
     2161 cataacaatt tgggttatac ctattttttg ctgggacaag atgagaaagc gataaaagcg
     2221 tttgagcaat ctttgtatct cgacccagaa aatgagagag cgcagtttaa tttaagcctg
     2281 gcgcatcaaa aaatatcaaa aagtggggaa tagcaatgcg attccagaaa cgtaagatcg
     2341 tcttagcgat tggttgtata tcggcattga ccgggtgcgc ttcgtatttc tcaggaaagg
     2401 aattttcagc gtatgaagtg aggcccgtta cgaatatcca acaggcagaa agcagcgcag
     2461 agaattttta tcatatcggc gaatattatc aacgaaaaaa acaatggctt aatgcgcttg
     2521 agtcttatca gaaagcgtta tcgttgcaaa ctgagtatgt agaagcccat aatgggctgg
     2581 ggattgttta tgcaacccta ggtcagtatg aattagcaat tgatcacttc aaaaaagcaa
     2641 tcaccttaga tgcttccaag agctatttgc ataataattt aggctacgct tattacttgc
     2701 aaggggtata tcatgaagca gagctttcat ttcaagctgc gatatccaat gatccccgta
     2761 atggaaaagc taaaaagaat ttaacgttag tccataaaaa aatgggagta agcgagaaat
     2821 tagctagaac gcttaaggcg acgagtaata gtgaaacgat gtcaacaaag tatccagcac
     2881 aactaacaca cgaaaatcat ctatcacaac aattagttca ggtcgcacca aacgtttacg
     2941 agtttagatc acagcgagaa actcagaata aggtggaaaa caagctagta agtattgtta
     3001 tgcatgatga gcaggagact caattacaaa aaaatattaa aagtctcgca caagggattg
     3061 aagtttcaaa tggtaatggt gtaacaggta tggcccggaa tgtggcaaca caattgaaca
     3121 atgctggatt aggccaagca cgactgacta atcatcaaac ctttaaacaa gtacagacag
     3181 agatatatca tcgtgtcagc gctcacgcgc tagcagaaaa aattacacag ttattgccag
     3241 ggcaaatcaa actgattgaa agtgatgatt tgcgagaaga tatcaatcta aaaatcttgc
     3301 taggccaaga tatcgctaat tttaaagcgt taagtcataa ctctcatcaa gtaacgaaag
     3361 tgatgtagtc gttctagttt taagcgctgc tttaagcagc gctaacaagt tgacttgcat
     3421 tttctgcaag tgtctttact tcttcgctga tactatcaat tttgttttca gaaataccaa
     3481 gattactcca ctcttgctta gtaatttctt gagccgtatg ttcattaatc caaaaattag
     3541 aaagtaattt ttcagcggta ttaacaagcc tgattattgg taatcctagt gtagcgtctg
     3601 ggtgatcggg ggtatggtga tagcgcatca cagcgatgag ctcttcaggt aagccccagt
     3661 gtattccaag ttgagcaccg atttccccat gatgaatacc gactatttcc tgttcgattg
     3721 caagtaaatc tgcgttagcc gtattattca attgctcgtg caagcgattg ctggtttctg
     3781 tgtccaaata actcagcgtg ctgtagccaa tatcatgcaa aagaccagct agaaaaatct
     3841 gatcatcaat gggacgaaga tttgctggca tatgccgggc aatagtacgc atggttgtcg
     3901 cgatagccat gctatgagcc catagatcca tcgatttcaa ttttccttca ggtaacttgg
     3961 ccaatactgt aatcgtagcc ataccgattg caatcgattt gacttgggtc aacccaagta
     4021 acattgcggc atcgctaacc gaggtaatcg tgcttggcat actaaaaaag gatgaattag
     4081 agaggccgac gattcgagca gaaatttggg ggtcttgtgt aatgagttta agtaattgag
     4141 cttcaccctc agtggtatct aacgggagtg ccagcatttt ttgagcaata actggcatag
     4201 cgggaagtga atcaatttcc ctcagggatt gtttaatttt atttggggct ggcatggttg
     4261 atttcctcca aagagcgctt taatcaagga tctaattcag atgaggttac tgaatttagg
     4321 gtaactttat gaattctacg ctaatcgttt tcgctagaat tgtagaacta ggccataatc
     4381 ttttgctatt ttgatataaa gcaatgaaaa tgtttcagaa cgaactactc tcgagttatc
     4441 cagtaaatag aatcatttta gtcggtgcga gcaatctgac gttatcgctt cgtatgatta
     4501 ttcagcatat tcaagggagt gtcggtggtc caagtgaaat attagttgcg gcagggcatg
     4561 ggcgctccta tggtttgagt agccaagtga tgattcggga gttacccggt attgttcggt
     4621 gtggtttatg ggaacaactc agcacgcgtg taattattcc aagttatgct ttattgacag
     4681 atattggcaa cgatattccc tatggcgtgt gtcctgagca aattctacaa tgggttacct
     4741 ggtgtgttga tgagttaaca aaggcatctg cacaaatcgt aattaccaac ttaccgatag
     4801 aatctattga agcgctatca aataggcgtt tccaattttt taggcattta ttttttccgt
     4861 catgtacctt atcgaggatc gaagtcgtta gccgagcaaa ggtggtacat caaggtatcg
     4921 ctacactcgc acaacagaaa caacttatcc tctgtgagca atctcgccaa tggatgggag
     4981 ccgatggttt tcatgtcgca tttatgaagc gagagcaggt ttttagacag ctatgtaatc
     5041 attttgtagg gactgatgaa gatttatcat cagtccctaa acagtcagag cttcgattaa
     5101 gttggcagca gcgacctcgt tttgcattgc aacgcgtttg gggtaaagaa aaatgctgcc
     5161 aacaaccaag cggtttatta tcgaatcaag cgaccgtatc gctttattaa tggtgctcaa
     5221 tactgatcaa gttcagggta attcactgtc gacaaaaagt gttaatggcg ttgttgcact
     5281 gatggtcaat gcactttttg accaatttcc ataaaacagt gcagtaatga atttattgac
     5341 actattcgct acgtcaacaa agaatgactg aggcgttagg acacaatttg tgcaggcggg
     5401 gcagtgctca tcacagaaac tgcaatgaaa atatttggga gagtagccac aaggccccca
     5461 actgtattgt ttcgcgacac gataaaatgc gcgtaaattc tgactgtgca gatacgcggc
     5521 ttttgctgct gcatttttat caacaaagca ggtagtaaca tcaaatcccc agtacattgc
     5581 aggtaaatta acattgccgg cattgtttgc attataggga tcgatgctaa atatggcatc
     5641 cgcttgcacc aacccatttg aaattgcatt ctgagcggcc tgaccacccg ctgagtgacc
     5701 tcccattagc caatgagtaa cagtgttgca accaatacca cttaaccagc tattgagatt
     5761 cgttttgacg tcgttagcca gggttgcata tttttgaggg tcagttttta ccatgttacc
     5821 aggtgcatga tccatgatta cagtaacgta tccgtacttg atcaattctt ctgataattt
     5881 gtcatagcta gagatactca ttgatgtacc cacgccgagt aaaattgcat cacagcttgc
     5941 aggtgtcgtg gatgaatagt gaattcgtac ttgaccgttg ggtgttgttt gatctaggta
     6001 gctcgcatga gcgtaatgaa caaccaatag aattggcaga acaattaatt taatgatgat
     6061 ttgatcgatt cgcattggaa tgattctcct tgatgaatga gttgaggtag tcaaggttat
     6121 tttgactaac ggttagcgct atgatgaaaa aaagcttaat caatgtagtg ctaatttttc
     6181 attgttgaat
//
LOCUS       DAOQKQ010000157         6017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_6792_length_6017_cov_6.518282, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000157 DAOQKQ010000000
VERSION     DAOQKQ010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 6017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6017
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6792_length_6017_cov_6.518282"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="PKZ49_09920"
     CDS             <1..278
                     /locus_tag="PKZ49_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HND36921.1"
                     /translation="SKRELRNRAKASLEQEARILEEVLSEAATGGVVIVPPLKPAIEA
                     RLGKPLALSTLYQMLARHGWRKLAPDKSHPQADPAAREAWKKNSPSD"
     gene            323..835
                     /locus_tag="PKZ49_09925"
     CDS             323..835
                     /locus_tag="PKZ49_09925"
                     /inference="COORDINATES: protein motif:HMM:NF024751.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HND36922.1"
                     /translation="MFQDEARFGRISDVRHCWDKKPNRPVVRAMLTQQYTYAYGAVSP
                     LDGRFDSLILPWVNGDCMQLFVDEISARYPHENIVMVMDGAGWHKSQTMKLAENMRIL
                     FLPPYSPELNPQEHVWDELREKFFHNRAFDSLDALEIHLESALKSFESNQETMRSITG
                     WDWIINAVSK"
     gene            1014..1382
                     /locus_tag="PKZ49_09930"
     CDS             1014..1382
                     /locus_tag="PKZ49_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HND36923.1"
                     /translation="MLPTTNRSFPMNKTELIDAIAARAQTTKTQTATILNGFMETVQK
                     TLAKGDTIQLVGFGTFLVAQRASREGRNPATGKTMTIPAKKVVKFRPGKALADAAAAT
                     KAPRKAQPAAKPKAAAAKRK"
     gene            1709..2503
                     /locus_tag="PKZ49_09935"
     CDS             1709..2503
                     /locus_tag="PKZ49_09935"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HND36924.1"
                     /translation="MDQNTFNLDKNQLVQHAFDQMEKHVKGADYTPRQKLALTCRILF
                     DNGHDSGLAGQITARSDQSGAFLTQRLGLGFDEISEANLLLVDEHLQVISGEGMPNPA
                     NRFHAWVYRARPDVNCIIHTHAIHTAALSMLEVPLQISHMDNCVLYDDVAFLPKWPGV
                     PVGNDEGELIAAALGNKRALLLAHHGLLVACSSIEEACLLALAFERAARMQLLATSVG
                     NIQPIDPVLGREAHDWILRAQRNAAAFAYYARRSLRLPHNQDCLAE"
     gene            2506..>6017
                     /locus_tag="PKZ49_09940"
     CDS             2506..>6017
                     /locus_tag="PKZ49_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HND36925.1"
                     /translation="MFHFTSLTQRFAVWFAVVALLPILLIGYSLLQTFEAEIKKAVIQ
                     QVSSIADKKIEQIDAYFNERMLDASVIQAADTTHQAIKEYTAAFKQFGVNSEAYRDLD
                     AIYRNHFERFVKHADYYDVFLISTDGWIVFTYSRESDFATSLLTGPYRDTGLGAVARR
                     ALREQKGSISDFEYYEPSKGAIAAFIAEPIIIEGEFSGVFALQIYSDPVFKVLTNNVG
                     LGQSGETILTRLESENTALVMAPLKKEPDAALKHKILLDDSRFSTGIRRSLAGQDGGG
                     LTIDYLGKEVVAAWRYLPLMKWGMVVHIDVSEAFASVYRVRAVGLVILFLTFIVATSG
                     ALLFNRRVVTPLKNLNEGAQAIASGNLNQRVPNEGWDEMEKLANAFNTMTERLNTSYR
                     GLEKRVAERTAELETANAELAIKEEETRSVVEHMVDCVVTTDEKGVMLSVNPVMEKLF
                     GYTPDEAIGQNVAILVPEPDRSQHEFYMERYCNTGHGQTYVGRPYLPGTHGIGLGREV
                     EGVHKNGQLIPVYLAVSEYFVGEKRHFTGVMRDIREHVKIMKDLEHARLEAEQANQAK
                     SAFLATMSHEIRTPMNGVIGMIEVLQQSSLSSYQMEMTNLIRESAFALLEIIEDILDF
                     SKIEAGKLEIEFAPIPLATVIEKACSMLEHLAANKGIELTLFIDPAIPDEVLGDALRL
                     RQIVVNLINNAIKFSSGQGRPGKVSTQVLLIEADHDQVMISIQVTDNGIGMNEETRER
                     LFIPFTQGDTSTTRRFGGTGLGLTITHHLVKLMGGEVTVQSKEGEGSTFIVHIPFKPL
                     PSETTTHQKQIDLAGLSCIVYGPDEGLSNDLVAYLKYAGANVERVTDLSAARKRIKTL
                     PAGQWLLVIDAGHDESSVDELRLAFRTRLNLDSPDKPHLVVVKRGRRHRGRIEKGEQV
                     TLDGNILYRFSFLHAVAVAAGRAKLEEDNLPLEGSSTRIIPPSREEALQQGRLILVAE
                     DNEINQKVIRQQLALLGHVADVVQDGRDALRHWESGDYAMILTDLHMPEMDGYQLTQA
                     IRAAKKGAIQIPIVALTANALKGEADRCRAIGMDDYLSKPVQLEQLKQTLQKWMPKHS
                     DDSKVPEQAKQQVINQQATPVDIRILQALVGDDPAITQEFLGDFKTNAQVISDQLHMA
                     HQQHQLDQVKAAAHKLKSSSRSVGA"
BASE COUNT         1668 a         1246 c         1503 g         1600 t
ORIGIN      
        1 gcagcaaacg tgagttacgt aaccgcgcca aagccagcct ggagcaggaa gccaggattc
       61 tagaggaagt gctgagtgag gccgccactg gtggcgtggt cattgttcca cccctgaagc
      121 cagcgattga agctaggctg ggaaagccac tggctttgtc cactctgtac cagatgctgg
      181 cgcggcatgg ctggcgcaaa ctggcccctg ataaaagcca tccacaagcg gacccagcag
      241 cgcgcgaggc atggaaaaaa aactccccga gcgactaacc gaggaagtgg cggcttttgg
      301 gaccccacgc ccgctgcgcc taatgtttca ggacgaagca cgtttcggtc gcatcagcga
      361 tgtgcgtcac tgctgggaca agaagcctaa tcgccccgta gtacgtgcca tgctgacgca
      421 acaatacact tacgcctatg gcgcggtcag cccactggat ggacgatttg attccttgat
      481 cctgccctgg gtgaacgggg actgcatgca actcttcgtg gatgaaatct cggcacgtta
      541 cccgcatgaa aatatcgtta tggtaatgga cggcgcaggc tggcacaaaa gccagaccat
      601 gaaattggca gagaatatgc gaatcctatt tctaccacca tactcgccag aactcaatcc
      661 acaggaacat gtgtgggatg aattacggga aaaattcttt cataaccgtg ctttcgatag
      721 tctagatgcg cttgagatac atctggaaag cgcactaaaa agctttgaat caaaccagga
      781 gactatgcgc agcattaccg gatgggattg gataattaat gctgtttcta aatagaattg
      841 gaataagtcc gattggaagc ctcaatgact agagaaatct ggggtaaaaa ccccagtttc
      901 ggtaattgta aggaaaaaag tgtcggaaaa gcgacaaaca aagcaaaatt ttgtttaaaa
      961 accccataaa catattgctt ttttgcgaaa tatcgggcga aaataccaac atcattttac
     1021 caaccacaaa taggagcttc ccaatgaaca aaacggaatt gattgatgca attgcagctc
     1081 gtgcacaaac aaccaaaaca caaactgcaa cgattctgaa tgggtttatg gaaaccgtac
     1141 aaaaaaccct agcaaaaggt gatacgattc agctagtcgg ctttggtacc tttctagttg
     1201 cgcaacgtgc gagtcgtgaa ggccgtaatc ctgcaacagg caaaaccatg acaattccag
     1261 ctaagaaagt agtgaaattt agaccaggaa aagcccttgc tgacgcagct gcagcaacta
     1321 aagcacctcg taaggctcaa cctgctgcta aacctaaagc tgctgctgcc aaacgtaaat
     1381 aatccccttt ttttcaaata atgatcttga gattgcattt ttgtgatctt gagagtctgg
     1441 taaaggccta cttgcaagct gtatcagcat tgacggatat cgcttaatac tatcaaactc
     1501 gtaggcctta tttttctact tactttcagt gttttgtaag caattttttg ctgagtacat
     1561 cttcttcata atactttcac acaagcttta gcttttcttc atgctttgcg ggcattattc
     1621 cattgaatgg ataatttatt tgctaagcac gatgtgattt gctactttga ttttaaattc
     1681 aattcttatt ttgtcagaga gcataatcat ggatcaaaat accttcaact tagataagaa
     1741 tcagctagta cagcatgcat tcgatcaaat ggaaaagcat gttaagggtg cagattatac
     1801 gccacggcag aaacttgcct taacctgccg catcttattt gataatggcc atgattctgg
     1861 gttggctggt caaattacgg cgcgatccga tcaatcaggc gcatttctaa cacaaagatt
     1921 aggcttgggt tttgatgaaa tcagcgaggc taatctttta ctggtcgatg agcatttgca
     1981 agtaataagc ggtgagggaa tgcctaatcc ggctaatcgt tttcatgctt gggtttatcg
     2041 agcccgccct gacgtaaatt gtattattca tacgcatgcg atccatacag ctgcgctgag
     2101 tatgctagaa gtaccattgc aaatctctca tatggacaac tgtgtgttat acgatgatgt
     2161 agcattcctg ccgaaatggc cgggtgttcc agtcggtaat gacgaaggtg aattgatcgc
     2221 agcagcgtta gggaataaaa gagcgttgct actcgcgcat catgggttgt tagtcgcgtg
     2281 tagtagtatc gaagaggctt gtttattggc acttgccttt gagcgggccg cacggatgca
     2341 attactggcc acatcagtcg gtaacattca acccatcgat cctgtgttag gaagagaagc
     2401 tcatgactgg attttacgtg cacagcgcaa cgctgctgca tttgcttatt atgcgcgacg
     2461 aagtctgaga ttgccccata accaggattg cttggcagag taatcttgtt tcatttcacc
     2521 tcccttacac agcgatttgc cgtatggttt gcggttgtcg cgttacttcc aatcttgctg
     2581 attgggtata gcctgttgca aacgtttgag gctgaaatca agaaggctgt cattcaacaa
     2641 gtttcttcaa tcgcagataa aaaaatcgaa caaatcgatg cctatttcaa tgaacgtatg
     2701 cttgacgcaa gtgtgatcca ggccgccgat acgacgcatc aagcgatcaa agaatacacg
     2761 gctgctttca agcaattcgg ggtaaattct gaggcatacc gagatctaga cgcgatctat
     2821 cgcaatcact ttgagcgttt tgtaaagcat gcagactact acgatgtttt cctgatttct
     2881 accgatgggt ggattgtctt tacctatagt cgcgaatctg attttgcgac gagtctactg
     2941 acaggaccct atcgtgatac cggactgggc gccgttgcta gacgcgcttt acgcgaacaa
     3001 aaagggagta tttctgattt tgagtattat gagccttcca aaggcgccat tgccgcattt
     3061 attgcggaac cgatcattat cgaaggtgaa tttagtggtg tttttgccct tcagatctac
     3121 agtgatcctg tatttaaggt tttaaccaat aatgtaggct tgggtcaaag tggtgagacg
     3181 attttgacaa ggttagagag tgaaaataca gccttggtca tggcaccatt gaagaaagag
     3241 cccgatgctg ccttaaaaca taaaatttta ttggatgatt cgagattctc aaccggtatt
     3301 cgacgcagct tggctgggca agatgggggt ggattgacta ttgattatct gggcaaggag
     3361 gttgttgctg cctggcgtta tctcccactt atgaaatggg gaatggtcgt tcacatcgat
     3421 gtcagcgaag catttgcttc ggtttatcgt gtgcgtgctg tcggtcttgt gattctgttt
     3481 ttgactttta tcgtggcgac atccggggcg ctattattca atcgccgcgt cgtgacaccg
     3541 ctcaaaaatc tgaatgaggg cgctcaagcg attgcatccg gaaatttgaa ccagcgcgta
     3601 cccaatgaag gctgggacga gatggaaaag cttgcaaacg catttaacac catgacggaa
     3661 cgtttgaata ccagctatcg ggggttagaa aagcgagtag cggaacggac tgctgaactt
     3721 gaaactgcaa atgctgaatt ggcgattaaa gaagaagaaa cgcgttcagt ggtcgaacat
     3781 atggtggact gcgtcgttac aaccgatgaa aagggagtga tgctttcagt taatcccgtg
     3841 atggaaaaac tgtttggcta tactcctgat gaggcgattg ggcaaaatgt agcgattctt
     3901 gtaccggaac cagatcgtag tcagcatgaa ttttatatgg agcgctactg taatacgggg
     3961 catgggcaaa cctatgtggg gcgaccttat ttgccaggaa cacatggtat tggtttgggg
     4021 cgagaggtgg aaggcgttca taaaaatggc caattgatcc cggtctatct tgcagtcagt
     4081 gagtattttg taggggagaa gcgtcatttt actggagtaa tgcgggatat tcgtgagcat
     4141 gtaaagatca tgaaagatct tgagcatgca cgactcgagg cggagcaggc aaatcaagcc
     4201 aaatcagcct ttctggcgac catgagtcat gagatccgga cgcctatgaa tggtgttatt
     4261 ggtatgatcg aagtgttgca gcaatccagt ctaagtagtt atcaaatgga gatgacgaat
     4321 ttaattcgtg aatctgcttt tgctttgttg gaaatcattg aggacattct tgatttctca
     4381 aaaattgaag caggtaagct tgaaattgaa tttgcaccga ttcccctggc cactgtgatt
     4441 gagaaagctt gcagcatgct cgagcatctg gctgccaata aagggattga attaacctta
     4501 ttcattgatc ctgccattcc cgatgaagtg ctcggagatg cgttgcgctt gcgtcagatc
     4561 gtcgtcaatc tcattaataa tgctattaaa ttctcaagtg gccagggacg acccggtaaa
     4621 gtttctaccc aagtgttatt gatcgaagca gatcatgatc aagtaatgat ttcgattcag
     4681 gttacggaca atgggattgg tatgaatgaa gaaaccaggg aacgattatt catccctttt
     4741 actcagggcg atacttcgac cacacgacgt ttcggtggga ctggcctggg tctcacgata
     4801 acgcatcatc tcgtgaaatt aatgggagga gaagttacag tccagagtaa agagggcgag
     4861 gggtctacgt ttattgttca tattcctttt aaaccgctgc cttcagaaac gactacgcac
     4921 cagaagcaga tcgatcttgc aggtctttcc tgtatcgtgt atgggcctga tgaaggattg
     4981 agcaatgatc tcgtcgccta tttgaaatat gcaggtgcaa atgttgagcg ggttactgat
     5041 ctatctgcag cacggaaacg aatcaaaaca ttaccggctg gacaatggtt actggtcatt
     5101 gatgcgggtc atgatgagtc atcggttgat gagttacggc ttgcatttcg tacccgtttg
     5161 aatttagatt cacctgataa gccgcatttg gtagtcgtta aacgcgggcg gcgccatcgg
     5221 gggcggattg agaagggtga acaagtaacg cttgacggta atattttata tcgtttttct
     5281 tttttgcatg ctgttgcggt tgccgcaggg cgagcaaaac ttgaggaaga taatttaccc
     5341 ttagaagggt catcgacccg aattattcct ccttctcgtg aagaggcatt gcagcagggt
     5401 agattgatat tggttgcaga agataatgaa attaaccaaa aagtaattcg gcaacaactt
     5461 gcgctacttg ggcatgttgc tgatgtagtg caagatggtc gtgatgcatt gcggcattgg
     5521 gaaagcggtg attatgctat gatactaacg gacctgcata tgccggaaat ggatggttat
     5581 cagttgacac aagctattcg tgctgcaaaa aagggagcga ttcagattcc aatcgttgca
     5641 ttgaccgcga atgcgcttaa gggagaggct gaccggtgcc gagcaatcgg tatggatgat
     5701 tatttgagta aaccggtaca gttagagcag cttaagcaaa cactacaaaa gtggatgcct
     5761 aagcacagtg acgattccaa agtgcctgag caagctaagc agcaggtaat taaccagcaa
     5821 gcaacgcctg ttgatattcg gatattgcaa gcattagtcg gggatgatcc agcgattact
     5881 caggaatttt taggtgattt taagacaaac gcacaggtaa ttagtgatca acttcatatg
     5941 gcgcatcaac aacatcaatt ggatcaggta aaggccgctg cacacaagct aaaatcctcg
     6001 tcgcgatcag taggggc
//
LOCUS       DAOQKQ010000158         6002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_6825_length_6002_cov_5.339163, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000158 DAOQKQ010000000
VERSION     DAOQKQ010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6825_length_6002_cov_5.339163"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /gene="nuoK"
                     /locus_tag="PKZ49_09945"
     CDS             complement(<1..184)
                     /gene="nuoK"
                     /locus_tag="PKZ49_09945"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HND36926.1"
                     /translation="MVSLSHYLILAAILFSIGVVGIFLNRKNVIIILMSIELMLLAVN
                     INFVAFSHFLQDTAGQI"
     gene            complement(249..848)
                     /locus_tag="PKZ49_09950"
     CDS             complement(249..848)
                     /locus_tag="PKZ49_09950"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HND36927.1"
                     /translation="MSAQDTIFYFFSAVLILSALGVITFRNPVYAALLLVLAFFSAAG
                     IWLLLEAEFLAIALVLVYVGAVMVLFLFVVMMLDINLDKLREGFWKWFPFGALIAVVI
                     AVEIGLVMMGDQFKQMIPLQPKASDYSNTKELGRLIYTEYVYVFELAAVVLLVAMVAA
                     IALTLRHRTDKKSPNPSSQVKVKRNDRIRMVSMAPETKE"
     gene            complement(859..1347)
                     /gene="nuoI"
                     /locus_tag="PKZ49_09955"
     CDS             complement(859..1347)
                     /gene="nuoI"
                     /locus_tag="PKZ49_09955"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HND36928.1"
                     /translation="MERIKHFFKSFLLFELLKGMKVTGRYLFAPKITINYPEEKTPQS
                     PRFRGLHALRRYPNGEERCIACKLCEAVCPAMAITIESEQREDGTRRTTRYDIDMIKC
                     IFCGFCEEACPVDAIVETRVLEYHGEVRGDLTYTKEMLLAVGDQYEQQIAKDREVEAR
                     YR"
     gene            complement(1360..2460)
                     /gene="nuoH"
                     /locus_tag="PKZ49_09960"
     CDS             complement(1360..2460)
                     /gene="nuoH"
                     /locus_tag="PKZ49_09960"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HND36929.1"
                     /translation="MEYIQQWFEQLFGATLGSTLFLFAENLVYIVAIVLPLLLGVAYL
                     TFAERKIIAYMQIRLGPNRVTFFGISWLRGWGQPIADAVKAIMKEIIVPTGANKFLFL
                     LAPVITIAPALAAWAVVPFSPELVLADINAGLLYILAMTSLGVYGVIIAGWASNSKYA
                     FLGSMRSAAQVVSYELAMGFALVCVLMMSSSLNLGDIVIGQQGDSFLNWYLIPLFPMF
                     LVYFISGVAETNRAPFDVAEGESEIVAGFHVDYSGMAFTVFFLAEYANMILVATLTSI
                     MFLGGWLPPFNFAPFTYIPGMVWLLLKIAFILFFFLWFRATFPRYRYDQIMRLGWKIF
                     IPITLVWIVLIGMVMQLPVSLRDTFPLNMWFS"
     gene            complement(2483..4873)
                     /gene="nuoG"
                     /locus_tag="PKZ49_09965"
     CDS             complement(2483..4873)
                     /gene="nuoG"
                     /locus_tag="PKZ49_09965"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HND36930.1"
                     /translation="MINIEINGKQLEVPKGSTVMDAAKQLGIYIPHFCYHKKLSIAAN
                     CRMCLVQIEKAPKPLPACATPATEGMKIFTHSDQAVLAQKGVMEFLLINHPLDCPICD
                     QGGECQLQDLAVGYGESASRYQEAKRVVTNKNLGPLISTDMTRCIHCTRCVRFGQEIA
                     GIMELGMSGRGEHSEILSFVGKTVDSELSGNVIDLCPVGALVSKPFRYSARTWELSRR
                     KSISPHCGLGSNLIVQVKQNRVMRILPRENEEINECWLSDKDRFSYEGLNSEDRLSKP
                     MIKRNNQWITCDWQEALLFVVQNLKACVSQYGGRAIGALGSPHSTLEELYLLQKLVRG
                     LGSNNIDHRLRQSDFRADNYQQGIPWLGMPIAEVSNLKSILIIGSNLRKDHPLLALRV
                     RQAVKSGAQLSIVNSTDDDLLTKVAQRAIVAPSELVRTLAQILKAIIEYRYVEIADKI
                     KETLTKVNVTDVTKAIANNLVSNQSAGIFLGNLSQHHPNYSEIFLLAQEIAKVTSAKL
                     GVFGEAANSVGAYVAGAIPNRKLGNLTTNGEEVGLNAIQMLNAEAVSACKAYILVNLE
                     PEYDTYDPQSALERIKGAEFVVSLSSYKGNITDYADVILPIAPFTETSGTFINTEGRV
                     QSFNGVVSPLAEARPAWKVLRVLANLLELKGFDYETSEQVCTELLSDYGNVADHLNNR
                     INEFTVNLNEANVNGIERIGEVNIYQSDPIVRRASSLQLTRDAHYPRAIISPSLMEKL
                     GLRVGDKITIKQGVGESQLEVEQSESIPDNCIRIASAFNQTATLGGMFGEVSVKRM"
     gene            complement(4928..>6002)
                     /gene="nuoF"
                     /locus_tag="PKZ49_09970"
     CDS             complement(4928..>6002)
                     /gene="nuoF"
                     /locus_tag="PKZ49_09970"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HND36931.1"
                     /translation="KWSFMPRQYEGEKYLVCNSDEGEPGTFKDRDIMRFNPHILIEGM
                     LIAAYAMGIRTGYNYVHGEIWDVYERMEEAVEEARSAGFLGNNILGSNFSFQLYNHHG
                     YGAYICGEETALLESIEGKKGQPRFKPPFPANFGLYGKPTTINNTETFASVPWIILNG
                     GEKYLELGKPNNGGTKIFSVSGHVNKPGNYEVPMGTPFAELLELAGGMRGGRKLKGCI
                     PGGSSMPILPGEVMMNTNMDYDSIAKAGSMLGSGAVIIMDETTCMVKALERLAYFYYE
                     ESCGQCTPCREGTGWLYRIINRIEHGKGRQEDLDLLDNLADNIQGRTICALGDAAAMP
                     VRAMLKHFRDEFAYHIEYKKCMV"
BASE COUNT         1895 a         1392 c          986 g         1729 t
ORIGIN      
        1 aaatttgacc cgctgtatct tgtagaaaat gagaaaaagc aacaaagttg atattaacag
       61 ccagtaacat tagctctata gacatcaaga tgataattac attttttcga ttgagaaaaa
      121 ttccaacgac accaatagaa aataaaattg cagcaagtat gaggtaatga gataaagaga
      181 ccaaaatttt cacctataat ttttattaat ttaccgccaa aatcctttat atcaacctat
      241 gatggcactc attcctttgt ttcgggtgcc atgctaacca ttctaatacg atcattcctt
      301 tttactttaa cctgactaga tgggttaggt gattttttat cggtacggtg ccttaatgtc
      361 aatgcaattg ctgcaaccat tgcaacaagt aaaacaactg ctgccaattc aaaaacataa
      421 acatattcag tataaatcaa tcgacctaat tccttggtat tactataatc cgaggctttc
      481 ggctgaagcg gaatcatctg cttaaattga tcccccatca ttactaaacc tatctcaaca
      541 gcaataacta ctgcgattag tgctccaaaa ggaaaccatt tccaaaaccc ttctcgtaat
      601 ttgtctagat tgatatctag catcatcact acaaacaaga ataaaaccat gactgcacct
      661 acataaacta agactaacgc aatggctaga aactcagctt ctaatagtaa ccatataccg
      721 gcagcagaaa aaaatgccaa taccaatagt aatgcagcat atactggatt tcggaacgta
      781 ataactccga gtgctgataa aatcagaaca gctgaaaaga aataaaaaat tgtatcttgt
      841 gcgctcatat ataaaaaatt atcggtagcg tgcctctact tctcgatcct ttgcaatttg
      901 ctgctcatat tggtctccaa ccgcgagcag catctctttt gtataagtta gatcaccacg
      961 aacttcacca tgatattcaa ggacacgggt ttcaacgatg gcatcaacag gacaagcttc
     1021 ctcacaaaat ccacaaaaga tgcatttgat catatcaata tcgtaacgtg tcgtgcgacg
     1081 agtaccatcc tcacgctgtt cggattcaat ggtaattgcc atcgcagggc aaacagcttc
     1141 gcatagttta catgctatgc aacgttcctc accattaggg taacgacgca gtgcatgtaa
     1201 tcccctgaaa cggggagact gtggcgtctt ttcttcaggg taattaatag taattttagg
     1261 tgcaaataaa tatcgcccag tgactttcat tccttttaat aactcaaata aaagaaatga
     1321 tttaaaaaag tgcttgattc gttccatata gttaatacct cagctaaacc acatattaag
     1381 agggaaagta tcacgtagtg aaaccggtaa ctgcataacc attccaatca aaactatcca
     1441 aaccaaggta attgggataa aaattttcca acctagcctc ataatctggt catatcgata
     1501 acgagggaat gttgcgcgaa accataaaaa gaaaaacaat ataaaggcaa tcttcagtaa
     1561 taaccaaacc attcctggta tataagtgaa tggtgcaaaa ttaaatggtg gtaaccaccc
     1621 gcccaagaac atgatgcttg tcaatgttgc gactaatatc atgtttgcgt attcagctag
     1681 aaaaaagaca gtaaaggcca tacctgaata atctacatga aatccagcaa cgatctcaga
     1741 ttcaccttct gctacatcaa agggagcacg attcgtttca gcaacaccag aaataaagta
     1801 aactaagaac atcggaaata atggtattaa ataccaattc agaaaactat ccccctgctg
     1861 ccctatcaca atatcaccaa gatttaagct actagacatc atgagcacgc aaaccagcgc
     1921 aaaccccatt gcgagctcat atgaaactac ctgggctgct gagcgcatcg atcccaggaa
     1981 agcatacttt gaattagaag cccacccggc aatgatgacg ccatacaccc ctaaagacgt
     2041 catagcaaga atataaagta aacccgcgtt gatatcggct aaaactaact ctggggagaa
     2101 aggaacaaca gcccatgcag caagcgcggg cgcaatcgta attactggtg caagaagaaa
     2161 taagaattta ttggctccag ttggaacaat aatttccttc ataattgctt ttactgcatc
     2221 agcgattggc tgtccccatc ctcttaacca agaaatcccg aagaaagtga ctcgattagg
     2281 ccccaaccta atttgcatat aagcaatgat ttttctttct gcaaacgtta agtaagcaac
     2341 gcctaaaagt aaaggcaaaa caattgcaac aatatagact agattctcag cgaacaaaaa
     2401 caaggttgat cccaaagtgg caccaaacag ttgctcaaac cattgttgta tatattccat
     2461 taacagacct taatttgctc aattacatcc gtttgaccga tacttctcca aacataccac
     2521 ctaatgtcgc agtctgatta aaagcacttg caatacgaat gcagttatca ggaatcgatt
     2581 cactctgctc aacctctagc tgactttcac caactccttg ctttattgtt attttatcac
     2641 cgactctcag tcctaatttc tccatcaagg aaggcgatat gattgctcga ggataatgag
     2701 catcacgcgt taattgtaaa gacgaggcac ggcgtacaat cggatcagac tgatagatat
     2761 ttacctctcc aatccgttcg ataccattca cattggcctc atttaaatta actgtaaact
     2821 cattgatcct attatttaaa tgatcagcta cattaccgta atcagacaaa agttcggtac
     2881 aaacttgctc agatgtttca taatcaaacc ctttcaattc cagcagattt gctaatactc
     2941 gcaaaacttt ccaagcgggt ctcgcttctg ctaagggaga aactaccccg ttaaagcttt
     3001 gaacacgccc ttctgtatta ataaatgttc ctgatgtttc tgtaaatggt gcaataggca
     3061 aaataacgtc agcataatcg gtgatgttac ctttataact actcagcgat actacaaatt
     3121 ctgcaccctt aattcgttca agcgctgatt gaggatcgta agtatcatat tctggttcta
     3181 aattcactag aatataggct ttacatgcac tgactgcttc agcattcagc atttggattg
     3241 catttaatcc aacctcttct ccatttgttg tcaaattacc caatttccta ttagggatcg
     3301 cacctgccac gtatgcgcct acgctattgg ctgcttcccc aaaaacaccc aatttggcgc
     3361 tagtaacttt ggctatttct tgtgcgagta aaaaaatctc actgtaatta ggatgatgct
     3421 gagataaatt cccaagaaaa attccagcag attgattcga tactaaatta tttgcaatcg
     3481 cttttgttac atccgtaacg tttacttttg taagcgtttc tttaatctta tctgcaatct
     3541 caacatatcg atactcaatt atcgctttaa gaatttgtgc taatgttctt accaattcag
     3601 aaggagcaac gattgcacgt tgagctactt tcgtcagcaa atcatcatca gtagaattaa
     3661 ctatacttaa ctgtgctcca cttttaactg cttgacgcac cctcaatgct aaaagtggat
     3721 gatctttccg caaattacta ccaataatta atatcgattt taaatttgaa acttcagcaa
     3781 taggcatacc taaccatggg atcccttgct ggtagttatc agcacgaaaa tcagattgtc
     3841 tcaagcgatg atcgatattg ttacttccca acccccttac taatttttgc aacagataaa
     3901 gttcttcaag cgtgctatga ggggaaccca gtgcgccaat cgctcttcca ccatattgac
     3961 taacacaagc tttcagattc tgaaccacaa ataacagcgc ttcttgccaa tcacaagtta
     4021 tccattgatt atttcttttg atcatcggct tagataagcg atcttcagaa ttcagacctt
     4081 cgtatgaaaa tcgatcttta tcggacaacc aacattcatt aatctcctca ttttctcgag
     4141 gcaaaatccg catgacacga ttttgtttaa cttgaacaat caaattgcta cctaacccac
     4201 aatgagggct gatcgatttt ctgcgagata attcccatgt tcgagctgaa tatcgaaatg
     4261 gcttactgac taatgctcca acaggacaaa gatcaatcac attccctgac agctcggaat
     4321 caactgtttt cccaacaaaa gatagaattt cagaatgctc accacggcca ctcatgccaa
     4381 gttccataat tccagcaatt tcttgcccaa atcgaacaca ccgtgtgcaa tgtatgcagc
     4441 gagtcatatc ggtggagata agtggcccaa gattcttatt cgtaacaaca cgctttgcct
     4501 cttgatagcg agaagcgctt tcaccataac caaccgcaag atcttgcagc tgacattcac
     4561 cgccttggtc acaaatcggg caatcgagtg ggtgattaat tagtaagaac tccataacac
     4621 ccttctgcgc tagaacagcc tggtcagaat gagtgaaaat tttcatccct tctgttgcag
     4681 gtgttgcaca tgctggcaaa ggtttagggg ctttctctat ttgcaccagg cacattctgc
     4741 aattcgctgc tatcgacaat ttcttatggt agcaaaaatg cgggatataa atcccaagct
     4801 gttttgcagc gtccattaca gtgctccctt tgggcacctc cagttgtttc ccattaattt
     4861 cgatattaat catcgatcag ctaactcaaa aattcttatg attgaacggt aaaattttta
     4921 ttacttatca caccatacat tttttgtatt caatgtgata agcaaattca tcgcgaaaat
     4981 gctttaacat agcccgtaca ggcattgctg cagcatctcc taatgcacaa atggttcttc
     5041 cttgtatgtt atcagcgaga ttatcaagta aatctaaatc ttcttgccta cctttcccat
     5101 gttcgatccg gttaataatc cgatacaacc atcctgttcc ctctctgcag ggtgtgcact
     5161 gaccacagga ttcttcataa tagaaatagg ctaatctttc cagtgcttta accatacacg
     5221 ttgtctcatc cataatgatc acagcgcctg agccaagcat agaaccagct ttcgcaattg
     5281 aatcataatc catatttgta ttcatcatca cctctcctgg caagattggc attgatgaac
     5341 cacctggaat acaacccttt aattttcgac ctccccgcat ccctcctgct aattctaata
     5401 attctgcaaa gggagtcccc attggcactt cataatttcc aggtttattg acatgtcctg
     5461 atactgaaaa aattttagtt ccgccattgt ttggttttcc taattctaaa tacttctctc
     5521 ccccattcag aataatccaa ggtacagatg cgaaagtttc tgtgttatta atcgtcgtag
     5581 gctttccata taagccaaaa tttgctggaa aaggcggttt aaatcgtggt tgcccctttt
     5641 ttccttctat tgattccaat aatgcagttt cctcaccgca tatgtatgca ccatagccat
     5701 gatgattata gagttggaag ctaaaattcg accctaaaat gttattacct aaaaaaccag
     5761 ctgagcgcgc ttcttcgacc gcttcttcca ttcgttcata gacatcccaa atttctccat
     5821 gaacatagtt gtatcccgtt ctaattccca tcgcataggc agcgatcaac atgccttcaa
     5881 ttaaaatatg tgggttaaat cgcataatat cgcgatcctt gaaagtaccc ggttcaccct
     5941 catcagagtt acaaaccaaa tatttctcgc cctcatactg acgaggcata aaactccatt
     6001 tc
//
LOCUS       DAOQKQ010000159         5995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_6842_length_5995_cov_6.316498, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000159 DAOQKQ010000000
VERSION     DAOQKQ010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5995
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6842_length_5995_cov_6.316498"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(193..1143)
                     /locus_tag="PKZ49_09975"
     CDS             complement(193..1143)
                     /locus_tag="PKZ49_09975"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HND36932.1"
                     /translation="MNHQLQLNKNGELLHLLTIEGLPASILTLILDTAESFTGVTERE
                     IKKIPLLRGKSIFNLFFEPSTRTRTTFEIAAKRLSADVLNLNIAVSAQSKGEALLDTV
                     NNLSAMHADMFVVRHSQSGAAHMIASHVKPDIHVINAGDGRHAHPTQALLDMFTIRRY
                     KQDFHNLRVAIVGDILHSRVARSQIHALSTLGVPEIRVVAPKTLLPNKVERLGVHVYH
                     DMEKGLRDVDVVIMLRLQNERMQGANLPSNEEYFKYYGLTPEKLAKAKPDAIVMHPGP
                     MNRGIEIDSVVADGGQSVILPQVTFGIAVRMAVMSILAGN"
     gene            complement(1140..1655)
                     /gene="pyrR"
                     /locus_tag="PKZ49_09980"
     CDS             complement(1140..1655)
                     /gene="pyrR"
                     /locus_tag="PKZ49_09980"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HND36933.1"
                     /translation="MSQRLPDAEFLLTELIKQLTPIAHESMAMVGIHTGGVWVAQRIH
                     QALNLKLPLGVLDISFYRDDYSKIGLHPQVKPSALPFEVENSHIILVDDVLYTGRTIR
                     AAVNEIFDYGRPASIHLAVLVDRGQRELPIAAQYVGAVLSLPTNNMLELKQQSDGRLS
                     LGLLDVKLAAE"
     gene            complement(1636..2046)
                     /gene="ruvX"
                     /locus_tag="PKZ49_09985"
     CDS             complement(1636..2046)
                     /gene="ruvX"
                     /locus_tag="PKZ49_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HND36934.1"
                     /translation="MAFDFGKRHIGIAIGESELKLAHPLTTITSEVIKIQFEAIAKLI
                     TAWEPKLLVVGLPLHDDGAEHEMTRLSRLFARRLEGRFRIKVVMVDERFTTRSARLTL
                     NELGIRRSQQDRFIDQVAAQHILQSFFDHVPTTP"
     gene            complement(2148..2708)
                     /locus_tag="PKZ49_09990"
     CDS             complement(2148..2708)
                     /locus_tag="PKZ49_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HND36935.1"
                     /translation="MENVNLTDHFLIAMPNMADPFFAKTLTYICEHNERGALGLVINR
                     PTDLSLEKFFDQLGIPRIDTFPKEPTVLFGGPVQIDSGFVLHKPIGNWKSTLAVNPKV
                     GLTSSVDIFHAIANCEGPDEMLVTLGYSGWAPGQIEQELAQNAWLTVPASHSIIFEMT
                     FDERLPAAIKLLGINFSNLSNQIGHA"
     gene            complement(2761..3387)
                     /gene="coq7"
                     /locus_tag="PKZ49_09995"
     CDS             complement(2761..3387)
                     /gene="coq7"
                     /locus_tag="PKZ49_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HND36936.1"
                     /translation="MFNIDRVIIGLDNALRTLAAPAQTYRPVPGDSLPDHELTEVEKK
                     KSIALMRVNHVGEICAQALYQGQALTARNQVVQKTLTQAAREETEHLAWTERRIAELG
                     GRKSLLNPVWYGGSFAIGLVAGMLGDKWNLGFLAETEHQVEKHLAGHLQQLPTQDEKS
                     RAIVTQMKTDEASHATMAVSHGGAELPLPVKLAMKASSKVMTQTAYWI"
     gene            complement(3423..4730)
                     /locus_tag="PKZ49_10000"
     CDS             complement(3423..4730)
                     /locus_tag="PKZ49_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HND36937.1"
                     /translation="MAITIKHYQFLFMETTRLEKPQASVASWNQTEILAEADKCVACG
                     LCLPHCPTYRLTLSEADSPRGRIAMISGVMKQHIPMNERFTLHIDRCLTCRACEYVCP
                     SKVSYGKLVDQARVKILNASVKKADVPYKIRSWLERQWIDKPWRFDTLRPFVKLFQQL
                     GLQRLLLRLPFLNGTSFRLLLSKLPPIRYPFQHWQSFYPAVGKQRGEVGLFLGCIARL
                     IDVETHLSTIAVLNQLGYSVHVPQQQTCCGALYQHRGESTHSGNLLAQNQQTFKDLNL
                     TALITTASGCGAHLVENNGFSIPVMDINQFLAEQDFSQIKFNPLAKRVAVHHPCTLRH
                     VLRGSQFPSKILAYIPEIELKELANQDQCCGAAGTYFLDQPGIAEALLTHKIQDVLAI
                     NADYLATSNIGCAFHIASQLNEQQAGIEVLHPVTLLARQMSIQ"
     gene            5089..5802
                     /locus_tag="PKZ49_10005"
     CDS             5089..5802
                     /locus_tag="PKZ49_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36938.1"
                     /translation="MNAAERIAEKYLQHMGIELVEFEPDGNVTPDFLVNKKIAVEVRR
                     FNQNYEDTTGNTLGLEETAIPLAQKFKQLLKSLGPSTNGETWFVTIDFSRPIEPWLIL
                     RPKIEVALNGLRLDASFKQRIIQVNDLVELYLIRASKDHGSAFVFGGYSDGDSGGLVM
                     AEVERNLRICIAEKEKKVAPHRHKYPEWWLLLVDYIDFGMEEDDRVVFKESVMPRIKH
                     SFDKVIFVNPLDHRYGFEV"
BASE COUNT         1643 a         1295 c         1343 g         1714 t
ORIGIN      
        1 tgggaatgct accaattgca acgattttgc cctcagcgat acagagatct ttaactgcat
       61 cgattttatt tttaggatcg atcaaacgtc catttttgat gagcaatttc ataaattgga
      121 tgggtgttta ctcaggtaat actattataa aggaaggtta tttctcgtca tgctaatagg
      181 tataagatcg cgctaattac cggctaaaat ggacatgaca gccatgcgca ccgcgattcc
      241 aaacgtaacc tgcggtaaaa tcactgattg cccgccatca gcaacgacag aatcaatctc
      301 gattcctcga ttcattgggc caggatgcat aacaatggca tctggctttg ctttagctaa
      361 tttctccggc gtgagtccgt aatatttgaa atattcttca ttgctcggta agtttgcgcc
      421 ttgcatgcgt tcattttgca agcgcagcat gatgaccacg tcgacatcgc gcaagccttt
      481 ttccatatca tggtaaacat gaacgcccag ccgctcgact ttgttgggta gtaatgtttt
      541 aggagcaacg actcgaattt ccggtacgcc gagcgtgctt agggcatgaa tctgtgatcg
      601 agccactctc gagtgcagaa tatcgccaac gatcgcaacg cgcagattat gaaaatcctg
      661 cttgtagcga cgaatcgtaa acatatcgag taacgcttga gtaggatgag catgtcggcc
      721 atcacccgcg ttgatgacat gaatatcggg ttttacatga cttgcaatca tatgcgctgc
      781 gccactttgc gaatggcgaa cgacaaacat gtctgcatgc attgcagaca ggttgttgac
      841 agtgtctaac aatgcttccc cttttgattg cgctgaaacc gcaatgttta ggttaagcac
      901 atctgcagat agacgttttg cagcaatttc aaatgttgtt cgagtacgtg tgctaggctc
      961 aaaaaaaaga ttgaatatcg atttacctcg taacagcggg atttttttga tttctctttc
     1021 cgtgacaccg gtaaaggact ctgctgtatc aagaatcaga gtcaaaatcg aagctggcaa
     1081 gccctcgata gtcagtaaat gaagtagctc accgttttta ttgagttgaa gttgatgatt
     1141 cattctgccg ccagcttcac atccagtaag cccaaactaa gtcttccatc ggattgttgc
     1201 ttaagttcta acatattatt ggtgggtaac gataatacag ccccaacata ctgagcagca
     1261 attggaagct ctcgctggcc gcgatcgact aacacagcca aatgaatact ggcaggtcgg
     1321 ccataatcga aaatttcgtt tacggccgca cggattgttc ttccggtata aagcacatca
     1381 tcgactaaaa tgatatggct attttcaact tcaaacggaa gggcagaggg tttgacctgg
     1441 ggatgcaggc caattttact ataatcatct cgataaaatg agatatccag gacgccaagg
     1501 ggtaatttaa ggttgagcgc ttggtgaatt cgctgtgcaa cccatacgcc tcccgtatga
     1561 atacccacca ttgccatcga ttcatgggca atcggtgtta attgcttgat cagctcagtg
     1621 agtaaaaact ctgcatcagg gagtcgttgg gacatgatca aagaaagatt gaagaatatg
     1681 ttgtgctgca acttgatcga taaaacgatc ttgttgcgag cggcggatac ctaattcatt
     1741 gagtgttaat ctcgcactgc gggtcgtaaa gcgctcatca accataacga ccttgattct
     1801 gaaacgacct tctaatcgcc ttgcaaataa tctgcttaat cgagtcattt catgctctgc
     1861 gccatcatca tgcagcggta atccaactac taataatttt ggttcccaag ctgtgataag
     1921 ttttgcgatg gcttcaaatt gtattttgat tacctcgcta gtgatcgtgg tcagcggatg
     1981 agccaatttc agctcgcttt caccaatagc aattccaata tggcgttttc caaaatcgaa
     2041 ggccatcacc gttccttgga aacaatactg ggaatcgtta atcgtactta aagtgctttc
     2101 cgagttgctc gttaccattg aacttagatg attactttgt ggctcattta agcgtgaccg
     2161 atttggttgg ataaatttga aaaattaata ccaagcaatt tgatcgctgc aggcaatcgt
     2221 tcatcaaagg tcatttcaaa aatgatgcta tgagaagcgg gtactgtaag ccaggcattc
     2281 tgggctaatt cttgttctat ttgacctggt gcccatcccg agtaaccgag agtgacgagc
     2341 atttcatcag ggccctcaca gtttgcaatg gcgtgaaaaa tatcaaccga ggaagttaat
     2401 ccaacttttg ggttgacagc aagtgtcgat ttccagtttc caatcggctt atgtaacacg
     2461 aagccactat cgatttgcac ggggccacca aatagaacag tcggttcttt aggaaaggta
     2521 tcaatccgag ggatgccgag ttggtcaaaa aatttctcta atgagagatc agtgggacga
     2581 ttgatgacta atcctaatgc cccacgctca ttgtgttcgc aaatataagt caatgttttg
     2641 gcaaaaaagg gatcggccat attcggcatg gcgataagga aatgatctgt taaattgaca
     2701 ttttccattg cttcaacgct caaaacattg tttttactgt tgcttggata cgattattca
     2761 ttaaatccaa tatgcggttt gcgtcattac tttcgagctg gctttcattg ctaacttgac
     2821 gggtaaaggc agctcggcac cgccatgaga aaccgccatc gttgcatggc ttgcttcatc
     2881 ggttttcatt tgtgtaacaa tcgcacgact cttttcatcc tgagtaggca attgctgaag
     2941 gtgtcctgct agatgctttt ccacttggtg ctcggtctcg gctagaaaac cgaggttcca
     3001 tttgtcccct agcataccag cgacaagacc gattgcaaat gatcccccat accatacggg
     3061 attgagtaaa cttttacgtc cgcctaattc agcgattctt ctttcggtcc atgcaaggtg
     3121 ctcggtttct tcccgagcgg cttgagtcag tgttttttgt accacttgat tacgtgcggt
     3181 taacgcttgc ccttgatata acgcttgggc acagatttct cccacatgat tgactcgcat
     3241 cagcgcaatc gatttttttt tctccacttc cgttagctca tgatctggca agctatcccc
     3301 gggaaccggg cgataggtct gagctggcgc ggcgagcgtg cgtaatgcat tatctagtcc
     3361 gataataacg cgatcgatat tgaacatctc ttccaagctc cttttcaata atagattttc
     3421 aatcattgta tactcatttg acgagcgagc aaggttactg gatgcaaaac ttcgattcca
     3481 gcttgctgtt cgtttaactg acttgcgata tggaatgcgc acccaatatt ggatgtggcg
     3541 agataatcag cattaattgc gagcacatcc tgtattttat gagttaacaa agcttctgca
     3601 atacctggct gatcaaggaa ataagtcccc gccgcaccgc aacattgatc ctgatttgct
     3661 aattctttca attcaatttc tgggatgtac gccagaattt ttgagggaaa ttgcgatccg
     3721 cgtaaaacat ggcgtaaggt acagggatga tgaaccgcaa cgcgtttcgc taaaggattg
     3781 aatttaatct gactgaaatc ttgttctgcg aggaattgat tgatatccat cacgggaatt
     3841 gaaaagccat tattctccac taagtgagca ccacagcccg atgcagtcgt gatcaatgcg
     3901 gtcagattca agtctttaaa agtttgctga ttctgtgcta gcaaatttcc ggaatgagtg
     3961 gattcaccac ggtgctgata taaggcgcca cagcaagttt gctgctgagg aacatgaacc
     4021 gagtagccca attgattcaa tactgcaatc gttgataaat gcgtttctac atcgattaat
     4081 cgcgcaatgc accctaaaaa caaaccgact tcaccgcgtt gtttcccaac cgcaggatag
     4141 aatgattgcc agtgctggaa gggataacgg atagggggta atttcgatag caataatcta
     4201 aatgaagttc cattcagaaa gggcaatcgt aacaataagc gctgtaagcc cagttgctgg
     4261 aataatttaa caaatgggcg aagcgtatca aagcgccagg gtttatcgat ccattgccgt
     4321 tcaagccagc ttcgtatctt ataggggaca tctgcttttt taaccgatgc attcaaaatt
     4381 ttgacccgag cttggtcaac tagctttccg taggacactt tacttggaca tacatattca
     4441 caggcccggc aagttaagca ccgatcgata tgcaaagtaa agcgctcatt catgggaatg
     4501 tgctgtttca tgaccccgct gatcatcgcg atgcggccgc gtggcgaatc tgcttcagac
     4561 agcgttaatc ggtaagttgg gcaatgtggc aggcaaagtc cgcaggcaac gcatttgtct
     4621 gcttcagcga gtatttcagt ttgattccag gatgcaactg aagcttgcgg tttttctaat
     4681 cgggtcgttt ccatgaataa aaattgataa tgttttattg ttattgccaa ttgctgctta
     4741 caaataggtt taggttgatt gtagccgatt tattagcacg taatagtaca cgcttcatca
     4801 gtcgtaattc agcgctacag atcattttac tgagcattga gctatcgtat cgaatctaac
     4861 tctgattgcc catcgaaact catttatgct gtacttggtc ccaaagcagg aacttgctcc
     4921 tcaggggttt gtttatgatt acaatttgtg ggtatatcaa agtttgttat gatgaaagct
     4981 aagtaaataa cacaaataca gagaatagcg tgcggtttac tgagttagat ttcatttttg
     5041 acgtaatggc ttgtatcatt ccgaacgtct gtttttagct aggcatttat gaatgcagca
     5101 gagcgcatcg ctgagaagta cctgcaacac atggggattg aacttgtaga gtttgagcct
     5161 gatggaaatg tcacccctga ttttcttgtt aacaagaaaa tcgcagtgga ggttcgaagg
     5221 ttcaaccaaa attacgagga tacgactggg aacacactcg gactggagga aacagctatc
     5281 cccctggctc aaaaattcaa acagctattg aaatcacttg gaccatctac aaatggggag
     5341 acttggttcg ttaccattga tttcagtaga cctatagagc cttggttaat ccttcgcccc
     5401 aagattgaag tggcactgaa tggtctcagg ctagacgcct cctttaagca gagaatcata
     5461 caggtaaacg acttagttga gctttacctc atccgagcaa gcaaggatca tggaagcgcc
     5521 ttcgtatttg gtggttacag cgacggagac tcaggtggtt tggttatggc agaggtagaa
     5581 agaaatctca gaatctgcat tgccgaaaag gagaagaaag ttgcgccaca ccgacacaag
     5641 tacccggaat ggtggctgct tctcgttgat tacatcgact tcggaatgga ggaagatgac
     5701 cgcgtcgtgt ttaaagagtc agtcatgccg cgcataaagc actctttcga caaggttatc
     5761 tttgtcaatc cactggatca tcgctatggg tttgaagttt aactagatgc tatagtgttc
     5821 aattactacg ttttcttagc taaaacgtaa gatgatgagt cgagctgact gtgcaggggt
     5881 tccatcactc acgatagtcc ttatttgcgc atcttatctt tgatatattt tgctaaaaga
     5941 aatcctgcga ccaatgcaat cactgcataa agaaccgttt caaataaacc cataa
//
LOCUS       DAOQKQ010000160         5983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_6868_length_5983_cov_5.774966, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000160 DAOQKQ010000000
VERSION     DAOQKQ010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5983
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6868_length_5983_cov_5.774966"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="PKZ49_10010"
     CDS             <1..674
                     /locus_tag="PKZ49_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HND36939.1"
                     /translation="PTNERLQFLPGQYIDILLKDGKRRSFSLANAPHDDEFLQLHARN
                     YPGGVFSEHVFTRMKEKDILRIEGPLGSFYLREPPSDKTPLIFLASGTGFAPIKSILE
                     HILSTLPFQESHRKLLLYWGVRVKADLYLNDLVEQWANTYKNFSYIPVLSDALPTDQW
                     KGRTGLVHQAVLEDIPNLASYQVYACGSPLMVKAAYQDFTQLRNLPAAQFFSDAFIPS
                     DNQMP"
     gene            complement(678..1856)
                     /locus_tag="PKZ49_10015"
     CDS             complement(678..1856)
                     /locus_tag="PKZ49_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HND36940.1"
                     /translation="MRLVLWVLFLFAAAVGIVYVAQFNSGSVLFNVPPYKVELTINNF
                     FILLIVAFFIFYVLLKILARILGFSFYFRRKRINDRTLVGLKAFFEGKYDRAQKAAAS
                     ALRLNSSPIINGINAALAARAAHKLEDYTARDEFLAASEEVAPGEHTLRLITQAELLL
                     EEGRHKEALDALHSLYSLGGLQSITALQLELKAQQMAQNWDAVMDLVNILANRYPYGK
                     GSIEQLKHHAQQESIKKNSTNLDLLNKYWHSLNSMEQLDSRLAATAAKGYIALNDMAT
                     AQRVIEQSLDSKLDNELITLYSKCLGNSVSWQIQRAENWLSKNPNNAELLLTLGKLCT
                     YCELWGKAQNYLEASLSIEPSRAAHLALAQLFEKLDKRELASDHYHKGLGFTLQKQAI
                     "
     gene            complement(1923..2630)
                     /locus_tag="PKZ49_10020"
     CDS             complement(1923..2630)
                     /locus_tag="PKZ49_10020"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF016275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HND36941.1"
                     /translation="MERIQHTKHRSSQQGALLAFLVLIIVLAVVGCFWAKKSGHLVDL
                     KHAIAGYLDKVDLTGKKPRQLLADIETELSNAEKQLGAIKATLSENDQAIAEKLASIS
                     QVIETLPLAMDRHFVKLDFALPHSETLQNSKLYLFLSEICKDLQKLISIQKIDDSSIE
                     IISPAQADQLRKNIQLKLTLAQLALSSEDRADLQSNLGEVVASMDRHFDKQAESVVEV
                     LKVLDSLHSYRLEDKES"
     gene            complement(2652..3416)
                     /locus_tag="PKZ49_10025"
     CDS             complement(2652..3416)
                     /locus_tag="PKZ49_10025"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HND36942.1"
                     /translation="MPALSGLSILVTRPAHQATYLVNRIKSLGGEPLLFPVLEITDVE
                     DVRPLLSIIDRLHEFDFAIFVSPNAVDKALPLICKNAPFPSHLKVAVVGKGSAQKLSS
                     YGIDKVILPAHRFDSEALLELEILKQVQQKRIVIFRGNEGRKLLGETLTNRSAIVEYA
                     ECYQRNKPKTDVSALLAYWSRGSVHAVTITSSEGLHNLFDMVGEVGQQLLKNTPFFTA
                     HERIAEIAKDLGLKDINLTESGDDGLIAGLLSYFNR"
     gene            complement(3439..>4139)
                     /gene="hemC"
                     /locus_tag="PKZ49_10030"
     CDS             complement(3439..>4139)
                     /gene="hemC"
                     /locus_tag="PKZ49_10030"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HND36943.1"
                     /translation="AVHSMKDVPMNLPPGFVLAAITEREDPRDAFVSNQYKNLSELPA
                     GSVVGTSSLRRESQLRARFPQLHIQPLRGNVQTRLRKLDEGQYAAIILAAAGLKRLDL
                     SYRITALLEPEQSLPAVGQGALGIECLDNRPDLVRLMEPLHDRETAQCVEAERAMSRV
                     LGGSCQVPLGGFAQITDNVLTLRGFVATPDGKRIVSDELNGKPESGATLGEQLAQNLM
                     AQGADKILAALVVS"
     assembly_gap    4140..4239
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4240..4538)
                     /locus_tag="PKZ49_10035"
     CDS             complement(<4240..4538)
                     /locus_tag="PKZ49_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HND36944.1"
                     /translation="MPNPAAIPSKIVIASRESLLAMWQANFIKNRLRELYPQTEISIL
                     GMTTQGDQILDVTLSKIGGKGLFIKELEQALADGRADIAVHSMKDVPMNLPPGFV"
     gene            4669..>5983
                     /locus_tag="PKZ49_10040"
     CDS             4669..>5983
                     /locus_tag="PKZ49_10040"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HND36945.1"
                     /translation="MDVDAGLNDNSDEDSEEGKNTPLRHDIRMLGRMLGDTLREHEGE
                     ATFNLVESIRQTAIRFRRDGDPVAGQELDAILNRLKNKETIAVVRAFSYFSMLSNIAE
                     DLHHNRRRRIHLRAGSPPQSGSVTLALERALQSGINGKALEDFFDKAVVSPVLTAHPT
                     EVQRRNILDCQLTIQRLLTERDRTQLTPNEARHNEEAIRAAIQILWQTRMLRSVRLTV
                     HNEIENGLIYYSYTFLSQVPYIYAKIEDLLERRLGDQAPPVASFLKIGSWIGGDRDGN
                     PFVTHQVMLHAAERHSALVFDYYINEVYRIGQTMSLAERLIKASDELEQLAAASPDIP
                     ASRIDEPYRKAFLGIYARLIATSQRLGHAITPRNQVKAGESYADSAELVHDLDIIIHS
                     LEKNKSHWIARGALRDLRRAADVFGFHLAPLDMRQHSKIHEQVIAD"
BASE COUNT         1687 a         1366 c         1186 g         1644 t
ORIGIN      
        1 ttcctacaaa tgaacgtttg caattcttgc caggtcaata tatcgatatt ttactcaaag
       61 acggcaaaag acgtagcttt tcattagcaa acgctcccca cgacgatgag tttttgcaac
      121 tgcatgcacg gaattaccct ggtggggttt tctctgagca tgttttcacc cggatgaagg
      181 aaaaagatat attgcgaatt gaagggccgt tgggatcatt ctatttgcgg gaacccccta
      241 gtgataaaac cccactaatt tttcttgcga gtggaaccgg tttcgccccg attaaaagca
      301 ttcttgagca tattctctct acacttcctt tccaagagag tcatcgcaag ttattgcttt
      361 actggggagt gcgcgtaaaa gcagatcttt acttaaatga cttggtcgaa cagtgggcta
      421 acacctacaa aaattttagc tatattccgg tattgtcgga tgctttaccc actgatcaat
      481 ggaaaggtag aaccggtcta gtccatcaag ccgtattgga ggacattccc aatctagcat
      541 cctatcaggt ttatgcctgc ggttctcctt taatggtcaa ggcagcttat caagatttca
      601 ctcaattgag aaatctaccc gccgcccagt tcttttctga tgcgtttatt ccctctgata
      661 atcaaatgcc ttagctttta tattgcctgc ttctgtaaag taaaacctaa acctttatga
      721 taatgatcag aagctagctc acgtttatcc agtttctcaa ataattgagc aagggccaaa
      781 tgcgctgcac gacttggttc aattgataaa cttgcttcta ggtaattttg tgctttaccc
      841 cacaattcac aataagtaca caacttacct aatgttagaa gaagctcagc attattagga
      901 tttttactca accagttctc tgcacgttga atttgccaac tgacactatt accaaggcat
      961 tttgaataaa gtgtaataag ctcattatct aatttgctgt ctagactttg ctcaatgact
     1021 ctttgtgcag ttgccatatc attcaacgcg atataacctt ttgcagctgt ggcagccaag
     1081 cgactgtcca attgctccat tgaattcaaa ctatgccagt atttattgag aagatctaag
     1141 ttcgtactat ttttcttgat gctctcttgc tgagcatggt gcttgagttg ctcaatcgag
     1201 cctttaccat aaggataacg atttgcaaga atattgacca aatccatgac tgcatcccag
     1261 ttttgggcca tttgctgcgc cttcaactcc aactgcaatg ccgtaatact ttgcaagcca
     1321 cctaaagaat ataaagaatg caacgcatca agcgcttctt tatgacgacc ttcctctagt
     1381 agcaactcag cttgggttat cagccgaagc gtatgttcac cgggcgcaac ttcttcagaa
     1441 gcagctaaga attcatcgcg tgccgtgtaa tcttctagct tatgcgctgc tcgtgctgcc
     1501 aatgctgcgt taataccatt aatgatcgga gaactgttca atctaagggc tgaggctgca
     1561 gctttctgag ctctatcata ttttccttca aaaaatgcct ttaagccaac cagggttcgg
     1621 tcattgattc ttttgcgtcg aaagtaaaaa ctaaacccta aaattcgcgc taggatttta
     1681 agcaaaacat agaaaataaa gaacgcaaca attaataaaa tgaaaaaatt gttaatcgtc
     1741 aactcaacct tataaggcgg cacattaaat aaaaccgagc cactattgaa ttgagcaaca
     1801 tatacaatcc caactgctgc tgcaaaaaga aataaaaccc aaagcactag tctcattcat
     1861 ctcctccctc atttgtaact tgaatatagt gtgccatttc gatggttctg aaataattgt
     1921 tattatgatt ctttgtcttc taaacgatag ctatgcaacg aatcaagtac tttgagcact
     1981 tcgacaactg attctgcttg tttatcaaag tgacggtcca ttgaagcaac gacctcgcca
     2041 agattagatt gaagatctgc acgatcttca gatgataaag ctagctgtgc tagcgtcagt
     2101 tttaattgta tattttttcg taactgatca gcttgagctg gcgagataat ttcaatgctc
     2161 gaatcatcga ttttctgaat actgatcagt ttttgaagat ctttgcaaat ttcagataag
     2221 aacaaatata gcttactatt ttgcaaagtt tcggaatgcg gcaatgcgaa atccaactta
     2281 acaaagtgac gatccatcgc aagcggtaaa gtctcgatca cttgagaaat ggatgcaagc
     2341 ttttcagcga ttgcttgatc attctcggag agcgtcgctt ttattgcacc aagttgcttt
     2401 tccgcattac tcaattcagt ttcaatatca gcgagtaact gacgcggctt cttccccgtt
     2461 aaatcaactt tatctaaata ccctgcaatt gcgtgtttca gatcgacaag atggccactc
     2521 ttctttgccc agaaacagcc caccactgcg agcacaatta tcagaacaag aaaagccaac
     2581 aaggcgccct gctgggaaga tcgatgttta gtatgctgga ttctttccat atttccaata
     2641 aaccatataa attaacgatt aaaataactc aacagacccg caatcaaacc atcatcaccg
     2701 ctctcagtca aattaatatc tttcaaacca agatctttcg ctatttctgc aatccgctca
     2761 tgcgcggtga aaaacggtgt attttttaac aattgttggc caacttcccc taccatgtca
     2821 aataaattgt gtaatccttc actactggtt atcgttactg catgaacaga tccacgcgac
     2881 caatacgcta gtaaagccga cacatcagtt tttggtttat tacgttgata gcactcagcg
     2941 tattcgacaa tagcactgcg attggttaag gtttcaccta ataattttcg cccttcatta
     3001 ccgcgaaaaa tcacgatacg tttttgctga acctgcttca gtatctcgag ctccagcaat
     3061 gcttcactat caaaccgatg cgctggtaag attactttat caatcccata agaagatagc
     3121 ttctgtgcgc tccccttacc gaccacagct acttttaggt gagaaggaaa aggggcattt
     3181 ttacaaatta agggtagtgc cttatcaacc gcgttagggc tgacaaaaat tgcaaaatca
     3241 aattcatgca gacgatcgat tatgctgagt aacggtcgaa catcctcaac atcggttatc
     3301 tcgagtaccg gaaataataa aggctcaccc cccagtgatt taattcggtt aacaagatag
     3361 gttgcctggt gagccggacg cgtgactaaa attgacaatc cagaaagcgc aggcatagga
     3421 attaatcatg cacagcggct atgagaccac aagtgcagct agtattttat cagcaccttg
     3481 tgccatcaag ttttgggcta gctgttcacc cagcgtagcc ccagactcag gtttcccatt
     3541 taattcatcg ctaacgattc tttttccatc tggagtcgct acaaacccac gcaaagtcaa
     3601 cacattgtcg gtaatttgcg caaaccctcc caatggtact tggcaacttc cacccaacac
     3661 tctactcatc gcgcgttcag cttctacaca ttgcgcggtt tccctatcgt gcaagggttc
     3721 catcaagcga actaaatcgg ggcgattatc aagacattcg attcctagtg caccctgacc
     3781 aactgcaggc aagctttgtt caggttctag taacgcagtg attcggtagg acaaatcgag
     3841 tcgtttcaaa cctgctgcag ctaagataat cgccgcatat tgaccttcat cgagcttacg
     3901 caatcgtgtt tgcacattcc cgcgcagcgg ttgaatgtgt aattgtggaa aacgtgcacg
     3961 aagctgcgat tccctcctta ggctcgatgt tccaacgaca ctgcccgcag gcaattctga
     4021 aaggttttta tattgattcg atacaaaggc atcgcgtgga tcttcacgtt ctgtgatagc
     4081 agccaagaca aaccctggtg gcaaattcat gggaacatct ttcatggaat gaaccgcaan
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna agacaaaccc tggtggcaaa
     4261 ttcatgggaa catctttcat ggaatgaacc gcaatatctg cgcgaccatc agcaagcgct
     4321 tgctcgagtt ctttgataaa caatcccttg ccaccaattt ttgacaaagt tacatccagg
     4381 atttgatcac cctgtgttgt cattccaagt atgctgattt cggtttgtgg gtataattcg
     4441 cgtaaacgat ttttaataaa attagcttgc cacattgcaa gaagactctc gcgcgaagca
     4501 attacaattt tagagggaat agctgctgga tttggcatgg tactgtattg aaaaatgact
     4561 gagcttattt aatttacgct cattctagca tgatccgttt taatataaac tgcacgaaaa
     4621 tagaataagc agtatttcga acacaggcat aaagaggggg tacgagtgat agatgttgac
     4681 gccggattaa acgataactc tgatgaagac tcagaggagg ggaaaaacac gcccctgcgg
     4741 cacgacatcc gaatgttggg ccgcatgctc ggagatacac tccgtgagca cgaaggggaa
     4801 gcgaccttca atctcgttga gtcaatcagg caaaccgcta ttcgtttccg acgtgatgga
     4861 gaccctgtag cgggccaaga gctcgatgcc attctcaatc gcctaaaaaa taaagaaact
     4921 atcgcagtgg tacgggcatt tagctatttt tctatgctgt ccaatatcgc tgaagattta
     4981 catcataacc gccgccgtcg tattcatttg cgagcgggat ctcctcccca gtccggtagc
     5041 gtaacactcg cattagaacg agcgctgcag tctggcatta atggcaaagc attagaagat
     5101 ttttttgaca aagcggtagt atcaccagtc cttacagctc acccaaccga agtccagcgc
     5161 agaaatattc tagattgcca acttacgatt caacgcttat tgaccgaacg ggaccgcact
     5221 caattgacgc ccaacgaagc gcgccataac gaagaggcga ttcgcgccgc aatccagatt
     5281 ttatggcaaa cgcgaatgtt acgttcggtg cgactaacgg tacacaatga aattgaaaat
     5341 ggcctcatct attatagtta taccttcctg agccaagtcc cctacattta tgcaaaaatt
     5401 gaagacttgc ttgaacgtcg cttaggggac caagcccctc ccgtagcatc ttttctgaaa
     5461 ataggtagct ggataggagg ggatcgggat ggtaatccat ttgtaacaca ccaggtcatg
     5521 ctgcatgcgg cggaacgcca ttcagctttg gtttttgatt attatatcaa tgaagtctat
     5581 cgcatcggcc aaacgatgag cttggccgag cgactgatta aagcaagcga tgaattagaa
     5641 caattagccg ctgcatcacc ggacattcct gctagccgca ttgatgaacc ttatcgaaaa
     5701 gcttttttag ggatctatgc acgtctgatt gccaccagtc aacgattggg gcatgcgatt
     5761 acgcctcgca atcaggtcaa agctggggaa tcctatgcgg atagtgcaga actcgttcac
     5821 gatctcgaca ttattatcca ttcgttagaa aagaataaat cgcattggat tgcaagaggc
     5881 gcattacgcg atttgcgtcg cgcagcggat gtattcgggt ttcacttggc acccttagat
     5941 atgcgccagc acagtaagat ccatgagcaa gttatcgcag atc
//
LOCUS       DAOQKQ010000161         5881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_7063_length_5881_cov_5.927394, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000161 DAOQKQ010000000
VERSION     DAOQKQ010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5881
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7063_length_5881_cov_5.927394"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PKZ49_10045"
     CDS             complement(<1..284)
                     /locus_tag="PKZ49_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36946.1"
                     /translation="MHEETSMHDLKPKGLKKPGRRKKYQGEVVQILEQIWEIYGRICS
                     KRLHPFLSEGIAVLERCHELSLSPELKQMLLSMSPATIDRCLQKARFTHP"
     gene            545..958
                     /locus_tag="PKZ49_10050"
     CDS             545..958
                     /locus_tag="PKZ49_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HND36947.1"
                     /translation="MESTFLQDNMFFIIAAVISGGMLLFPMISRSAAKEINVQQAVKL
                     INYENALVLDVRDDGEFAEGHLPNAKHIPVERINDRLQELEKYKNNPIVIVFRPGLRA
                     GHAASVLRKNGFDKVYNLNGGIDFWKRDNMPIVKK"
     gene            983..1240
                     /gene="grxC"
                     /locus_tag="PKZ49_10055"
     CDS             983..1240
                     /gene="grxC"
                     /locus_tag="PKZ49_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HND36948.1"
                     /translation="MAKVVMYVTGSCPYCVMAEKLLRSKGVEVIDKIRVDLQPDQRIE
                     MMNKTGRRTVPQIYIGDTHVGGYDDLVKLDQQGGVVKLLSA"
     gene            1309..1782
                     /gene="secB"
                     /locus_tag="PKZ49_10060"
     CDS             1309..1782
                     /gene="secB"
                     /locus_tag="PKZ49_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HND36949.1"
                     /translation="MTDQQPIFAIEKIYVKDLSLEIPNAPRVFLDREAPEINIQLNNQ
                     DTHFDGDFYEVQLTITVTARLKENTMFLVEIHQAGIFRIKNIPEKDMGSVLGVVCPNI
                     LFPYLRESVSDTVTRAGFPPVILNPVNFEAIYLQKQQANAAATVAQQSPGTEQIH"
     gene            1797..2273
                     /locus_tag="PKZ49_10065"
     CDS             1797..2273
                     /locus_tag="PKZ49_10065"
                     /inference="COORDINATES: protein motif:HMM:NF018095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HND36950.1"
                     /translation="MCNNHRNLTLILRFFWIVLFSLHASISIAADENEFLSISSNSAI
                     MYDAPSLKAEKLFIVNAYFPVQVLVKVEGWTKVKDSSDTIAWIENKFLTERRYVIVTA
                     PLANIHQSADIHSPILFQVQKDVVIELLDSSTTDWVQIRLQNGQMGYIRSNQIWGS"
     gene            2270..3259
                     /locus_tag="PKZ49_10070"
     CDS             2270..3259
                     /locus_tag="PKZ49_10070"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HND36951.1"
                     /translation="MKLAILGAGAWGTALAINFSASHPVTLWTRNTDQKAEIERQRVN
                     QRYLPGVALPDTLKLTCELNAAIEAVDLALVTIPIAGLRQLLEQLYKLNKSLPIILCC
                     KGFESPSTRLPHEIVQEVYAGEGSYAILSGPSFAKEVAQQRPTALTLASKDHTFLKII
                     SSQIRTPYLRIYASTDIIGVETGGATKNVIAIAAGISDGLELGSNARAALITRGLAEI
                     TRLGVSLGGHKETFMGLAGLGDLILTCTGNLSRNRQVGLRLAAGMQLADILQDIGHVA
                     EGVHTVQAVLTKGKLHKIEMPITQAVFSVLYEGVSAKEAVRKILEREPKMEVE"
     gene            3574..4806
                     /locus_tag="PKZ49_10075"
     CDS             3574..4806
                     /locus_tag="PKZ49_10075"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HND36952.1"
                     /translation="MFSLKHSGKKGLLVITLASLFMLFTEVQANPRYAAIVIDATTGT
                     VLHEENADEARYIASLTKMMTLYLLFESIEQKRMNLDTDMRVSAFAASMPPTNIRLQA
                     GNTLSVRDAIKALIVRSANDVAVVVAEALAGSEARFSQLMTNKARQLGMYATTFRNAS
                     GLPNKEQVTSARDMSILSTRLMKDFPQHYHFFSTQSFRHRGITYNSHNRLLRQFDGAD
                     GLKTGFIRASGFHLATSAKRDNHRLVAVVMGGETASARDQRVAELLNRNFDRLANPGS
                     TPAISSAKVLPITEHHRIKTTIPAQKSAALNNQTNESAVKQLSKPQRVTYSPTPRPNK
                     IWGIQLGSYSAHDRAHAHAKAATRWAPGEVAVTMVKISNRKLYRAQLVGLQENQARSA
                     CQNLSRQGIDCMVVRAHG"
     gene            complement(4843..>5881)
                     /locus_tag="PKZ49_10080"
     CDS             complement(4843..>5881)
                     /locus_tag="PKZ49_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36953.1"
                     /translation="AIDHASAFFNQERIANDTIVTHSVGSVFVVEQFFTRWITHLSAP
                     TFVFLTGAAMALSISQRSARGISIQAIDRDLLLRGLFIALLDLIVLSWFSGSLYLQVL
                     YAIGVSIIAMRYLRYLSSATLLACALALFIGGEAFTNLFWEPHDDVPLWFALTFAPWF
                     TPTFTVLYPAIPWLSIMMLGWVFGDWLTQSPDIRKVSPARMLFLSGIAAIICFLIVRW
                     LNGYGNMLLYREDFSLIQWLHVSKYPPSLSYISLELGLMAIILAALMWIKPKITVNQN
                     GPILVFGQTALFFYLAHFIVLGLLTIFLEKAGLQQAYLAALITLLILYPVCRLYRMLK
                     WRYPDSLLRFV"
BASE COUNT         1805 a         1265 c         1219 g         1592 t
ORIGIN      
        1 tgaggatggg taaaccgggc tttttgtaag caacgatcta tcgtagccgg gctcatactc
       61 aataacattt gctttagttc gggagatagg ctaagttcat gacatcgttc cagaaccgcg
      121 attccttctg acagaaaggg atgcaaccgt tttgaacaaa ttcgaccata gatttcccaa
      181 atttgttcca gaatttgtac cacttcgccc tggtatttct ttcgccgccc tggctttttc
      241 aatcctttgg gctttaaatc atgcattgat gtttcttcgt gcatttattc tttctcagtg
      301 acttgcagcg taaaataaaa aagtttgata gttgcgacgg aaaagtagca aacaatttgc
      361 atctagtcaa aaatgcggtt ggttgattga tagcggttgt cgaacagtag tagttctaat
      421 ggcgtattgc actaaaacgg atttatcttc gtatttaagc atcgtgattt actaatactt
      481 tgctaaaata gccaatcaaa caaaaccagc aattagatca aaactgaact cggaataaac
      541 ttatatggaa tcaacgtttc ttcaggataa tatgtttttt attatcgctg ctgttatcag
      601 cggcggtatg ttactttttc ctatgatttc tcgtagtgct gctaaagaaa tcaatgtgca
      661 gcaagctgta aaactcatca attatgaaaa tgccttggta ttagatgttc gcgacgatgg
      721 agaatttgcg gaggggcatc ttcccaatgc aaaacatatc ccggttgagc gaattaatga
      781 tcgcttgcaa gagcttgaaa aatacaaaaa taacccaatc gtgatcgttt ttcgtcctgg
      841 acttcgcgca ggtcatgccg cttcagtatt aagaaaaaat ggcttcgaca aggtttacaa
      901 tctaaacggc ggaattgatt tctggaaacg ggacaatatg ccgatcgtca aaaagtaacg
      961 agtagcggca attaatcata aaatggctaa ggtagtaatg tacgtgacgg ggtcttgccc
     1021 ctactgtgtc atggctgaga aactattacg ttctaaaggt gttgaggtca tcgataaaat
     1081 tcgagttgac ttacaacctg atcagcgtat tgaaatgatg aataaaactg gtcgcagaac
     1141 agtacctcaa atctatattg gagataccca tgtagggggt tatgacgact tggttaagct
     1201 tgatcaacaa gggggtgttg tcaaattact atccgcttag ttatttagac tcctcacgcc
     1261 gcactaaatt tattagtaaa cattgtcctc aaacatagga aattcaatat gaccgatcag
     1321 caaccgattt ttgcgattga aaaaatttat gtcaaagatt tatctttaga aatccctaac
     1381 gcaccgcgtg tctttctgga tcgggaagca ccagaaatta atatacaact caataatcaa
     1441 gatacccatt ttgacggaga tttttatgaa gttcagttaa cgatcacggt tacagcacgc
     1501 ctcaaagaaa acaccatgtt tttagttgaa attcatcagg ccggtatttt tcgcataaaa
     1561 aatattcctg aaaaggatat gggttcagtt ctaggcgttg tctgtcccaa tatacttttc
     1621 ccttatttaa gagaaagtgt ctctgataca gtaacgcgag caggtttccc gccggttatc
     1681 cttaatccgg tcaattttga agcgatctat cttcagaagc agcaagcaaa tgcggcagca
     1741 acagtagcac agcaatcccc tggaacagaa caaatacact aaaaggaacg atccgggtgt
     1801 gtaataacca tcgtaatcta acattgattt tacgtttttt ttggatagta cttttcagct
     1861 tacatgcatc cataagcatt gctgcagatg agaacgaatt tttgtctatc tcgagcaatt
     1921 cagcgatcat gtatgatgca ccttctttga aggctgaaaa attatttatc gttaatgcct
     1981 attttcctgt acaagttttg gtcaaagtcg aaggatggac aaaagtcaaa gatagtagtg
     2041 acactatcgc ttggattgaa aataagtttc tcactgagcg acgctatgtc atcgtaacag
     2101 cccctctcgc caatattcac cagtctgctg atattcactc acccattttg tttcaagttc
     2161 agaaagatgt cgtgatcgag ttactggatt ccagcactac agactgggtg cagatacgcc
     2221 tccagaatgg ccagatgggt tatattcgat caaatcaaat ctggggctca tgaaacttgc
     2281 gattctgggt gctggcgcct ggggtaccgc attagccatc aacttttctg ctagccaccc
     2341 agttacgcta tggacacgaa acacagatca gaaagccgag atcgaacgtc agcgggtcaa
     2401 tcaacgttac ctcccaggag tagcgctacc tgacacccta aaattaacat gtgaacttaa
     2461 tgccgctatc gaagcggttg atttagcctt agttactatt ccgattgctg gattacgcca
     2521 attactcgaa cagctttata agctcaacaa gtcactgcca atcattctgt gctgcaaagg
     2581 ttttgaatcg ccatccacgc gattacccca tgaaattgtg caggaagttt atgccggtga
     2641 aggttcatat gcgattttgt cagggccaag ttttgctaag gaagttgctc aacagcgccc
     2701 cactgcactt acattagcct ctaaagatca tactttttta aaaataatta gcagtcaaat
     2761 tcgtacgcct tatttacgga tttacgctag caccgatatc atcggcgtag aaacaggagg
     2821 agcgactaaa aatgtaatcg ccatcgctgc gggtatatcg gacggattgg aattaggaag
     2881 caatgcccgt gctgcgctaa ttacccgcgg actcgccgaa ataacccggt taggtgtttc
     2941 tttaggtgga cacaaggaaa cattcatggg gctcgctgga ttaggtgatt taatcctaac
     3001 ctgcacaggt aacttatcac gcaatcgcca ggttgggtta aggttagcgg cagggatgca
     3061 attagcggat attctccaag atattgggca tgttgctgaa ggcgttcaca ctgttcaagc
     3121 cgtgctcaca aaaggaaaat tacataaaat tgagatgcca atcacacaag cggtattcag
     3181 tgtcttatat gaaggtgtgt cagcgaagga agcggtacga aaaattctcg agcgggaacc
     3241 caaaatggaa gttgaataat gtttcaggca cctctgcaac aatcgtttag taaaaatttt
     3301 gtagctatag ccccctaaaa atagccatgg ctagtagcaa attaaaataa aatatcgcaa
     3361 aatacggcga taatgcagaa caaagttagc taaacttgct attagaaagg atttgaaagt
     3421 ttaaatcgtt gttataaggc tgttttatgc cctttttcta gcataaaaat ggttacaagc
     3481 gtgataaggt aggatcggac gcagttaact taacgcttgc ttcgcgtcat tactagaatt
     3541 tacgaagcat ttaggatacg gagaatattt tttatgtttt ctttgaaaca ttcagggaaa
     3601 aaaggattac tggttattac gttagcttcg ctattcatgc tatttaccga agtacaagcg
     3661 aatcctcgtt atgcggctat cgtcattgat gcaacgaccg gcacagtgct ccatgaagaa
     3721 aatgccgatg aagcacgcta tattgcttcg ttgactaaaa tgatgacgct ctatctgctc
     3781 ttcgaatcga ttgagcaaaa gcgaatgaat cttgatactg atatgcgcgt atcagccttt
     3841 gctgcttcca tgccacctac caatatacga ctacaagccg gcaatacctt gagtgtacgc
     3901 gacgccatca aagcgctgat tgttcgctct gctaatgatg ttgcagtcgt tgttgcagaa
     3961 gcactcgctg gtagcgaagc tcgatttagc caattgatga ccaacaaagc gcgccagctt
     4021 ggtatgtatg ctacgacatt ccggaacgct tcaggactcc ctaataaaga gcaagttact
     4081 tcggccagag atatgagcat actatcaacg cgcctcatga aagacttccc tcagcactat
     4141 cattttttct cgacccaatc atttcgtcac agaggcatta cctacaatag tcataaccgc
     4201 ctactgcgtc agtttgatgg ggcagatgga ttaaaaactg gctttattcg tgcttctggg
     4261 tttcatttag cgacttcggc aaaacgcgat aaccatcgac ttgttgctgt cgttatgggc
     4321 ggagaaacag catccgctcg ggatcaaaga gtcgctgaat tgctcaatcg aaatttcgat
     4381 cgactagcta acccaggctc tacaccagcg atcagcagcg ctaaggttct accgataaca
     4441 gaacatcacc gcattaaaac cacgattccg gcacaaaaat ccgcagcgct aaacaatcaa
     4501 accaatgaat cagcagtaaa acagcttagt aaaccacaac gcgtcactta ttctccaaca
     4561 ccaagaccca ataaaatctg gggtattcaa ttgggttctt attccgctca cgatcgtgca
     4621 catgcccacg caaaagcagc tactcgatgg gcacctggtg aagtagctgt tacgatggtc
     4681 aagatcagta accgaaaatt ataccgcgct caactggtcg gtttacaaga aaatcaagcg
     4741 cggtcagctt gtcagaatct ttctcggcaa ggcattgatt gcatggttgt ccgtgcgcat
     4801 ggttaagttc tcagaaaaaa gataacgcat aaccaatttt atttaaacaa aacgcaacaa
     4861 actatctgga taacgccact tcagcatacg atataaccta caaactggat aaagaattaa
     4921 taatgtaatt agtgcggcaa gataggcttg ttgcaatcca gctttctcca agaaaatcgt
     4981 tagtagacca agcactataa agtgcgctaa atagaagaat agcgcggttt gcccaaatac
     5041 aagaataggt ccattctgat tgactgtaat tttcggtttt atccacatta aagcagctaa
     5101 tataattgcc atcaacccaa gctccagtga aatatagctt aggcttggtg gatatttact
     5161 gacgtgaagc cactgaatca gtgagaaatc ttcacgatac agtagcatgt ttccataacc
     5221 attcagccag cgcacgatca agaagcagat gatcgctgca atcccagaca aaaataacat
     5281 cctagctgga gatacctttc ttatatccgg tgattgcgtt aaccaatcac caaaaaccca
     5341 accaagcatc atgattgaaa gccacggtat cgcgggatat agcaccgtga atgttggagt
     5401 gaaccaaggt gcaaatgtta gtgcaaacca aagcggcaca tcatcgtgag gctcccaaaa
     5461 aagatttgtg aacgcttcgc caccgatgaa taaagccaat gcgcaagcta gcaatgttgc
     5521 actacttaag taacggagat agcgcatcgc aataatgctg accccaatcg cgtataacac
     5581 ttgtaaataa agtgatccag aaaaccaaga tagtacgatc aaatcgagca atgcaataaa
     5641 taaccctctt aacaacaaat cgcgatcgat tgcttggatt gatatccccc ttgcgcttcg
     5701 ttgtgaaata ctcagcgcca tcgcagcgcc tgttaaaaaa acaaaagtag gtgcacttaa
     5761 atgcgttatc caacgtgtaa agaattgctc taccacaaaa acagaaccaa cactatgcgt
     5821 cacaatggta tcgtttgcaa ttcgctcctg attaaaaaaa gccgatgcgt gatcaatcgc
     5881 c
//
LOCUS       DAOQKQ010000162         5822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_7177_length_5822_cov_4.847928, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000162 DAOQKQ010000000
VERSION     DAOQKQ010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5822
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7177_length_5822_cov_4.847928"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PKZ49_10085"
     CDS             <1..229
                     /locus_tag="PKZ49_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HND36954.1"
                     /translation="HSFFLGIMTYVGWVLQLGVLYYLSLGVVVALVIYQYQLIKDRKP
                     PGCFKAFLHNNWVGASIFAGIALDSLIKIPF"
     gene            251..1714
                     /gene="ubiD"
                     /locus_tag="PKZ49_10090"
     CDS             251..1714
                     /gene="ubiD"
                     /locus_tag="PKZ49_10090"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HND36955.1"
                     /translation="MKYKDLRDFLDQLALRGQLKRVTHPIDPYLEMTEICDRLLKQGG
                     PAVLFEQPKNHSIPVLGNLFGTPDRVALGMGQESMAALREVGKLLAYLKEPDPPKGLK
                     DAWEKLPVLKQVLNMAPKVVSSAPCQEIVWEGQAVDLSKLPIQTCWPGDVAPLITWGL
                     TITRGPHKSRQNLGIYRQQVIAPNKVIMRWLAHRGGALDYRDFCQVNPGMPYPVAVAL
                     GADPATILGAVTPVPDSLSEYQFAGLLRGAKTEVVQCLTHDLQVPASAEIVLEGFIYP
                     DETAIEGPYGDHTGYYNEQERFPVFTIERMTMRRNPIYHSTYTGKPPDEPAILGVALN
                     EVFVPLLQKQFTEITDFYLPPEGCSYRMAIVSMKKQYPGHAKRVLFGVWSFLRQFMYT
                     KFIIVTDEDIDIRDWKEVIWAITTRVDPARDTLIVENTPIDYLDFASPVSGLGGKMGL
                     DATNKWPGETVREWGRTITMDAAVKSRIDQLWDELPF"
     gene            1980..2405
                     /gene="ndk"
                     /locus_tag="PKZ49_10095"
     CDS             1980..2405
                     /gene="ndk"
                     /locus_tag="PKZ49_10095"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HND36956.1"
                     /translation="MAIERTLSIIKPDAVAKNVIGQIYTRFENAGLKIIAAKMMLLSR
                     TEAETFYAIHRQRPFFNDLVSFMTSGPVMVQVLEGEDAIKKNRELMGATDPKKADKGT
                     IRADFADSIDANAVHGSDAAETASVEIACFFPALSIFSR"
     gene            2439..3602
                     /locus_tag="PKZ49_10100"
     CDS             2439..3602
                     /locus_tag="PKZ49_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HND36957.1"
                     /translation="MNSVVNLLDYDSAGLVRFCEEIGEKPYRAKQLLRWIHQFGKSDF
                     AEMSDLAKLFREKLHSLATIRSPEVMSDYTAQDGTRKWLLATGQGNAVEMVYIPEVAR
                     NTLCISSQVGCALNCSFCATGKQGFNRNLTVAEIIGQLWWANKTLKSQRQPASMQTDH
                     RPDEEVVGFTEQKPASDRVITNVVLMGMGEPLANFDNVVTALDLMLDDSAYGLSRRRV
                     TVSTSGIVPAMDRLRERCPVALAVSLHAPNDELRNELVPINKKYPLHELLAACKRYLQ
                     ASPRDFITFEYVMLDQVNDTVQHARQLVELVKDTPCKFNLIPFNSFSDSGYKRSTKEA
                     IHSFRDVLMQAGLVTTVRKTRGDDIAAACGQLAGNVLDKTRRTQRGAEGMASV"
     gene            3595..4386
                     /gene="pilW"
                     /locus_tag="PKZ49_10105"
     CDS             3595..4386
                     /gene="pilW"
                     /locus_tag="PKZ49_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HND36958.1"
                     /translation="MFKRLTALLISLSLAWLTGCVSPPDPESNLTPEEKRKRVQDRAK
                     IHTELAGQYYSRGQYRVAIEEAEIALRANNNYAPAYNVLGLIYMDLREDGRAQVNFER
                     AIRIAPNDADVHNNYGWFLCRNPAQIDLAIDHFMQAIKDPFYEIPEKSYTNAGLCVLK
                     RNDFEQAKAYLQKALVIHPDYPLAVLGLIQIDYLSRNLRVAQTNITRYIQSYPLSADS
                     LWLAIRIEHALGNDQAVASYGAQLQRRFPDSNEARSLREGKLRDE"
     gene            4379..5374
                     /locus_tag="PKZ49_10110"
     CDS             4379..5374
                     /locus_tag="PKZ49_10110"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HND36959.1"
                     /translation="MNENNGDNEMIASNGESMMSRSEEMDNLNNRALPESIGQILRDE
                     RIKRNLSIGEVSRRLRLSEQKIAAIEENDFSKLPSGTFLRGFIRNYANFLQLDFNSLI
                     PLLSQSAGVIVQQNSNRQSKTIPLESRRLGYSGSAYRLFISAFIVLSIMLLGYGIYNY
                     GDWGEYFVVKNGGDTSNTQADSLKQENGQTTMELSLPLPNLPSTSWNTVESFKPINEK
                     PVTTESSSLPTPVTTEIKETAQTNVLESSTDSGEKTVQLTFSRESWVEIRDSKKKVIF
                     MKTNARGSEQVVKGTPPLYLVIGNASGVGLTYNGKLVDLAPYTRKADDVARFSLE"
     gene            5424..>5822
                     /locus_tag="PKZ49_10115"
     CDS             5424..>5822
                     /locus_tag="PKZ49_10115"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HND36960.1"
                     /translation="MLRRKSVGVRVGSTVIGGDAPVVVQSMTNTDTADEVATAIQVAQ
                     LARAGSELVRITVNTTEAASAVASIRSRLDDMGCHVPLIGDFHFNGHKLLTAYPACAK
                     ALAKYRINPGNVGHGKKRDEQFATLIETACH"
BASE COUNT         1596 a         1108 c         1417 g         1701 t
ORIGIN      
        1 ccatagtttt tttctgggga taatgactta tgttggctgg gtattgcagc tcggtgttct
       61 ctattatcta tctctcggtg tagtggtcgc attggtcatt tatcaatacc aattgatcaa
      121 ggatcggaaa ccgccgggtt gttttaaagc tttcttgcat aataattggg ttggggcttc
      181 gatatttgct ggaattgctt tggattcgtt aatcaaaata cccttttaag caagtgactg
      241 cgaggtttcg atgaaatata aggatttgcg tgatttttta gaccaactgg cactgcgggg
      301 tcagctgaaa cgggtgacgc atcccatcga tccctatttg gagatgacag agatttgcga
      361 tcgtttattg aaacaagggg gccccgctgt tttatttgag caacctaaaa atcattcgat
      421 accggtactt ggaaatttgt ttggtactcc cgatcgagtt gcgctaggaa tggggcaaga
      481 atcgatggca gctttacgag aggttgggaa actattggct tacctcaagg agcccgatcc
      541 accgaaagga ttgaaggatg cttgggagaa actgccggtt cttaagcaag ttttgaatat
      601 ggcacctaaa gtggtatcca gcgccccctg ccaagaaatc gtgtgggaag gccaagcggt
      661 tgatttatcg aaattaccga ttcaaacctg ttggcctggc gatgttgcac cgttgattac
      721 ctgggggctt acgattacgc gaggtcccca taaatctcgc cagaatctgg gaatttatcg
      781 tcagcaagtg attgcgccta ataaagtaat catgcgctgg ctcgcgcatc gcggtggagc
      841 gctggactat cgtgactttt gccaagttaa tcccggcatg ccttatcctg ttgccgtagc
      901 actaggtgct gatcctgcca cgatactggg tgcggtgaca cctgttccgg atagtttgag
      961 tgagtatcaa tttgcaggac tattacgcgg tgccaagacg gaggttgtgc agtgtttaac
     1021 ccatgattta caggtacctg cgagtgctga aattgttttg gaggggttta tttacccgga
     1081 tgaaaccgca attgaaggtc cttatggtga tcataccggc tactacaatg agcaagagcg
     1141 ctttcctgtg tttacaatcg aacgcatgac gatgcggcgt aatcctatat atcattcgac
     1201 ttataccggt aaaccgccgg atgaacctgc gattttgggg gtggccctta atgaggtgtt
     1261 cgtgccgcta ttacaaaaac aatttactga aattacggat ttctatttgc cgccagaagg
     1321 ttgttcttac cgcatggcaa ttgttagcat gaagaagcag tatccggggc atgcaaagcg
     1381 tgtgttgttt ggcgtttgga gctttcttcg ccaattcatg tataccaaat tcattatcgt
     1441 aacggatgaa gatatcgata ttcgtgactg gaaagaggta atttgggcga ttacgacacg
     1501 cgttgatcca gcaagggata ccttgatcgt cgaaaatacc cccatcgatt atttagattt
     1561 tgctagccct gtttctggat taggggggaa aatggggcta gatgctacca acaagtggcc
     1621 tggagaaaca gtgcgtgagt ggggtagaac gattactatg gatgcggcgg ttaaatcacg
     1681 gattgatcag ctttgggatg aattaccttt ctaggttaaa agttacccaa taatttatcc
     1741 tcattgctaa cctttaaatc accagtctaa ttgtactgaa agtggctgag cccattgagt
     1801 actggatttg tgattgatat tccatcacga tacaggtgac atgttagaat atggcctttt
     1861 tatgcagctt ttataggcaa tatcgatcta accgagaaac ggattccaga atacggcatt
     1921 cattttttgc taaaatagtt gcttattatc aatcagttta tttttagtgg agttatttaa
     1981 tggctataga aagaacgctg tcgattatca aaccagacgc agtggcaaaa aatgttattg
     2041 gtcaaattta tacccggttt gagaacgctg gattaaagat tattgcagcg aaaatgatgt
     2101 tgctctcccg aacagaagca gaaacatttt atgctattca ccggcaacgt ccttttttta
     2161 acgatttagt cagctttatg acttcagggc ccgtcatggt acaggtacta gaaggggaag
     2221 atgcaatcaa aaagaatcgt gaattaatgg gagcaaccga tcctaagaaa gcggataaag
     2281 ggacgattcg tgctgatttt gcagatagta tcgatgccaa tgcagtgcat ggttcagatg
     2341 ccgcagaaac agcaagtgtc gaaattgctt gttttttccc agcattatct attttctctc
     2401 gttaattcga tcaattactg caaacgcatt tgttacgtat gaattcggtt gttaatttgc
     2461 ttgattacga ttcagctggt cttgttcgtt tctgcgaaga aatcggcgaa aaaccttatc
     2521 gtgccaagca gctactcaga tggattcatc aatttgggaa atccgatttt gcagaaatga
     2581 gcgatttggc aaaattattc cgcgaaaaac ttcattcatt agcgacgatt cgttcaccgg
     2641 aagtaatgag tgattacacg gcgcaagatg gaactcggaa atggttgtta gcgacggggc
     2701 agggtaatgc agttgagatg gtttatattc ctgaagtagc gcggaacaca ttgtgcatat
     2761 ccagtcaggt tggttgtgca cttaattgta gcttttgtgc aactggtaag caggggttca
     2821 atcgcaatct aacggttgca gaaatcattg ggcagttatg gtgggctaat aaaacgctta
     2881 aatcccagag gcaaccggct tcaatgcaga ctgatcatag gcctgatgag gaagtagtgg
     2941 gttttacaga acaaaagcca gcttctgatc gggtaatcac gaatgtcgta ttaatgggta
     3001 tgggcgagcc attggctaat tttgataacg tagttactgc gttagattta atgctcgatg
     3061 atagtgctta tgggttatcg agaagaagag ttactgtcag tacatcgggt attgtacccg
     3121 ccatggatcg gttacgagaa cgatgccctg tagccttagc cgtttctttg catgcaccta
     3181 atgacgaatt acgcaacgaa ttagtaccaa ttaataaaaa atatccgctt catgaattgc
     3241 ttgcggcctg taagcgttat ttgcaggcat ctcctaggga ttttattact tttgagtacg
     3301 tgatgcttga tcaagtcaat gataccgtcc agcatgcgcg tcaattggta gagttggtta
     3361 aagatactcc ctgtaagttt aatttgattc catttaattc cttttccgac tccggttata
     3421 aacgctcgac gaaggaggcg attcattctt ttcgtgatgt tttgatgcaa gctgggttgg
     3481 taaccaccgt tcgtaaaaca cggggagatg atatcgccgc tgcttgtggt caattagctg
     3541 gtaacgtttt ggataagacg cgacgaactc agcgtggcgc tgaaggaatg gcaagtgttt
     3601 aaacgcttaa cagctttatt gattagcttg agtttggcgt ggcttacagg atgtgttagc
     3661 ccacctgatc ctgaaagtaa tcttacacct gaagaaaaaa ggaagcgagt ccaagaccgc
     3721 gctaagattc ataccgaatt ggcggggcaa tattacagtc gtggtcagta tcgtgttgcc
     3781 attgaagaag cagagattgc cttgcgcgct aataataatt atgcaccagc ctataatgtt
     3841 ttagggctga tctatatgga tttgcgtgaa gatgggcgtg cgcaagtaaa ctttgaacgt
     3901 gcaatcagaa tcgcacctaa cgatgcggat gtacataaca actatggctg gtttctgtgt
     3961 cgcaatcctg ctcaaattga tttggctatt gatcatttta tgcaagcgat taaagatcca
     4021 ttctatgaaa tacctgagaa atcctatacc aatgcaggtt tgtgtgtttt gaagcgcaat
     4081 gattttgagc aagctaaagc ttatttacag aaagcgcttg tcatccaccc cgattatccc
     4141 ttggcggtat tgggattaat tcagattgac tatctgagta gaaatttaag agtagctcaa
     4201 accaatatta cgcgctatat tcagtcttat ccgctctcag cggacagttt gtggttagcg
     4261 atccgaattg agcatgcatt gggaaatgat caagcagttg cgagctatgg cgctcagttg
     4321 caaaggcgtt ttcctgattc caatgaagcg aggtcattgc gcgagggtaa attgagagat
     4381 gaatgaaaac aatggtgaca acgagatgat tgctagcaac ggtgaatcaa tgatgtctcg
     4441 aagtgaggag atggataatc taaataatcg cgcactacca gagagtattg ggcaaatatt
     4501 gcgagatgaa cggatcaaac gtaacttgag cattggtgag gtatcgcgtc ggttacgctt
     4561 gtcagagcag aaaattgctg caatcgagga aaatgatttt agtaaattac cttctggcac
     4621 ttttttacgt ggctttatcc gtaattatgc caattttctt caactcgatt ttaattcact
     4681 tattccttta ttatcgcaat cagccggggt aatcgttcaa caaaactcga acaggcagtc
     4741 taaaaccatt cctctcgaat caagacgtct agggtattcc ggcagtgcat atcgcttgtt
     4801 tatatccgct ttcattgttt tatcgattat gttactgggt tacggtattt ataactatgg
     4861 tgattggggt gagtattttg ttgtgaagaa tgggggtgat acttcaaaca cccaagcaga
     4921 ttccttgaag caagagaatg gtcaaactac gatggagcta tccttgccat tgcctaatct
     4981 tccatcaact tcttggaata cggtggagtc attcaaacct atcaatgaga aacctgtaac
     5041 gacggaatca tcctcattac ctacgccagt tactactgaa atcaaagaaa ccgctcagac
     5101 taatgtgtta gaaagttcaa ctgatagtgg tgaaaagaca gttcagctta ccttttctag
     5161 agagtcttgg gtagaaataa gagatagtaa gaagaaggtc atttttatga aaactaacgc
     5221 aagagggtct gaacaagtcg ttaaaggaac gcccccgctt tatttggtta ttggtaatgc
     5281 gtcgggggtt ggacttactt ataatggaaa gctagttgat ttggcacctt atactcggaa
     5341 agctgatgat gtagcacgct tttcactcga gtgaacgcct gtcaaatgtg taattatccc
     5401 taataaaata aaatcataat gttatgttaa gaaggaaaag tgtcggtgtt cgtgtaggtt
     5461 ctacagtcat tggaggggat gcgcccgttg tcgttcaatc gatgaccaat acggatactg
     5521 cagatgaagt tgcaaccgcg attcaagttg cgcaattggc gagagcgggc tctgaactgg
     5581 tgcgaattac tgtcaatacg actgaggccg caagcgctgt agcgagcata cgttcacgat
     5641 tagatgatat gggatgtcat gttccactga tcggggactt tcattttaat ggccataaat
     5701 tactaaccgc ttatcctgct tgcgctaagg ccttagcaaa gtaccgcatt aatcctggta
     5761 acgtaggtca cggtaaaaaa cgtgacgagc aatttgctac cctgattgag actgcttgcc
     5821 at
//
LOCUS       DAOQKQ010000163         5810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_7197_length_5810_cov_5.317116, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000163 DAOQKQ010000000
VERSION     DAOQKQ010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5810
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7197_length_5810_cov_5.317116"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1202
                     /locus_tag="PKZ49_10120"
     CDS             <1..1202
                     /locus_tag="PKZ49_10120"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HND36961.1"
                     /translation="KSLRHCALDFDPNRKNFLLWCGTADVWRITPPNPLSTFGWKAEM
                     EPAIPSDQVPPLNVGTGILGKWDYIPGFDVFMGLENETIGNIWVYKPIGWVTPDPDGN
                     GGGGGGPINQLPNVTITSPVNNQTISIGTPVTLSALASDSDGSIMEIKFFANNVFVDS
                     LTTEPYSTGWIPPQAGEYIIKAEAFDNLGAKVTSAPITINVVSDTPLTTTLLQRDSAG
                     TIGTADTYLSAYHPSTNFGGSQNLYLSGKNYVPLIKFNIFSHEGGPIPDNAIIESATL
                     HLYKGSSYSLLASLHAMNKPWNEKEATWAKASSSLSWNALGAAAAGLDYVAIADAQVN
                     LPWIADIWIALDVTQRVKTFSAGSVNYGWRLLFNSGDSVNNIRFNSSEKSPELDKRPK
                     LEIKWRI"
     gene            1477..2802
                     /gene="rimO"
                     /locus_tag="PKZ49_10125"
     CDS             1477..2802
                     /gene="rimO"
                     /locus_tag="PKZ49_10125"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HND36962.1"
                     /translation="MPTINKIPRVGFISLGCPKATVDSERILTRLRAEGYLISPSYQD
                     SDLVVVNTCGFIDSAIEESLDTIGEALRENGKVVVTGCLGAKGDTIKQVHPNVLSVTG
                     PQATEEVMEAIHIHLPKPHDPYVDLVPPQGIRLTPQHYAYLKISEGCNHRCSFCIIPS
                     MRGDLVSRSVGDIMQEAQNLVDAGVKELLVISQDTSAYGVDLKYRTGFWGGKPIKTRI
                     TELATALGELGVWVRLHYVYPYPHVDELIPLMSDLKIIPYLDIPFQHANARILKLMKR
                     PANSENVLARIQQWRTICPEITLRSTFIVGFPGETEQEFQELLDFLEIAQLDRVGAFM
                     YSPVEGAAANNLPDHISPEIQEERLARFMALQEKISADKLKQKIGKTLEVLVDEVDSE
                     GAIARSHADAPEIDGLVYIDADQKTIQQGEFVKVRIVNSDQHDLWAELV"
     gene            2845..3297
                     /gene="sixA"
                     /locus_tag="PKZ49_10130"
     CDS             2845..3297
                     /gene="sixA"
                     /locus_tag="PKZ49_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HND36963.1"
                     /translation="MELILWRHAEAEDGVPDASRRLTEKGMKQAQSMAQWLKPRLPKH
                     TRIIVSPAKRTQQTVAALCDTYETLEAVGTSTTADKVLATVNWPYEEGAVIVVGHQPT
                     LGQIAAAILGNPYASFSVKKGSIWWLSSKAKDLSSNGSLRLVMVPDMI"
     gene            3479..4669
                     /locus_tag="PKZ49_10135"
     CDS             3479..4669
                     /locus_tag="PKZ49_10135"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HND36964.1"
                     /translation="MDIDIVSALSGLIDLPWWGYIVVTLVLTHITIASITIFLHRHQA
                     HRALDLHPIPSHFFRFWLWLTSGMVTKEWAAIHRKHHAKCETLDDPHSPQIYGIKKVL
                     LEGSELYRKEGKNLETLERYGHGTPDDWMERNVYTKHSVAGVASMLIINVVLFGPIGI
                     TIWAVQMLWTPVMAAGIINGVGHYWGYRNFRAEDASRNIVPWGILIGGEELHNNHHAY
                     PTSARLSNKWFEFDIGWMYIRILEMMGLAKVKKIAPKLNLNESKTECDFDTLQAVIAH
                     RYEVLAKYTQSLKAILKNEMEQLLQVAAHHGVDGLTLKRWVLADASTLQDEERNKLSL
                     VLSKTKQLDKVYTMREELSAIWQRSSASKDELVKQLEDWCYRAEQSGIEALEKFSRRL
                     RCYA"
     gene            4886..5305
                     /locus_tag="PKZ49_10140"
     CDS             4886..5305
                     /locus_tag="PKZ49_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HND36965.1"
                     /translation="MNLIERAKELVSLTGDRATANRVRTLSILLAAKRALTHREIADQ
                     LLDAKKQLDRVTLYRILEWMSKKSLIHKIAGDDRVWRFRINTEIRAHQHAHFKCNRCA
                     KVICLKDSKYNPTPLLPVGYLLQEIELTIKGVCSECI"
     gene            5345..>5810
                     /locus_tag="PKZ49_10145"
     CDS             5345..>5810
                     /locus_tag="PKZ49_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HND36966.1"
                     /translation="MHTNNATKLHYWCAMLLINLIHMMIRRTTNSFRLSRNIPQRWLI
                     LSLVGSCWLSAVAFAQVEDTRKMIRMPELVITSTPFKDRSELNMTQPTSILQGDNLRR
                     KREASLGDTLINEVGVTSSSFGPGAGRPIIRALDGPRIQVLENGIGSLDVSSL"
BASE COUNT         1697 a         1015 c         1341 g         1757 t
ORIGIN      
        1 gtaaatcact ccggcactgc gcacttgatt ttgatcccaa tcgtaaaaac tttttattgt
       61 ggtgtggaac tgccgatgtt tggcggataa caccaccaaa tccgctctct acatttggtt
      121 ggaaagctga aatggaaccc gctattccct cagatcaagt tccgcctctt aatgtgggga
      181 caggaattct tggaaaatgg gattatatac ctggattcga tgtatttatg gggttagaaa
      241 atgaaacgat tgggaatata tgggtttata aaccgatagg gtgggttaca ccagatccgg
      301 atggtaatgg cggcggtgga ggtggcccaa ttaatcaatt gcccaacgtt actataactt
      361 cgcccgttaa taaccaaaca ataagtattg gaactccagt aacgttatct gcattggcta
      421 gtgatagcga cggttctatt atggaaatta aattttttgc gaataacgta tttgttgact
      481 cgttgacaac tgaaccttat tccacgggat ggattcctcc acaagctgga gagtatatta
      541 tcaaagcaga agcatttgat aatttgggcg caaaagtaac aagtgctcca atcacaatca
      601 acgtagtatc agatactcct ttaactacga cattactaca acgtgattct gctggaacga
      661 ttggtactgc tgatacctat ttatcggctt accatcctag cactaatttt gggggttctc
      721 agaatttata tttaagtggt aaaaactatg ttcctttgat taagtttaat attttctcac
      781 atgaaggtgg cccgatacca gataacgcaa tcatagaatc tgcaacgctg catctgtaca
      841 agggatcatc ttactctctt ttggcttcgc tgcatgctat gaataaaccg tggaatgaaa
      901 aggaagcgac ctgggccaaa gcttcaagta gcttatcttg gaatgcttta ggtgctgcgg
      961 ctgcagggct ggattatgtt gcaattgctg atgctcaagt aaatttacct tggattgcag
     1021 atatttggat cgcacttgat gtaactcaga gagtaaaaac gttctcagca ggtagcgtta
     1081 attatgggtg gagattgctg tttaattctg gtgattcggt taataacatt cgatttaatt
     1141 cgagtgaaaa gagtccagaa ttagataaac gtcctaagct ggagattaaa tggcgtattt
     1201 aattttatga tggctactac attatttagt taacgagttg tgttaatttt acaaagctct
     1261 agattggctg ctgttaaagg tcgatctaga gtttagcttt ttgataaaat agttatttca
     1321 tttactactt tagattcttt aatttattga atactctgtg tcagtttttt tacttctaga
     1381 atattaatac agagttccta gagcgtatgt ggccgtagtg tatgaatata ttctgtggtt
     1441 acctacataa aaggcaacaa tcgctaataa aaatatatgc ctactataaa taaaattccc
     1501 cgcgtgggct ttatttcgct tggttgcccc aaagcgactg tggattcgga acgaatatta
     1561 acgcgtctac gtgctgaagg ttatttgatt tcacccagtt atcaagattc tgacctggtc
     1621 gttgttaata cttgtggatt tattgatagt gcaattgaag aatcactgga tacgattgga
     1681 gaagcgctaa gggaaaatgg taaagtagtt gtcaccggtt gtttaggtgc aaaaggagat
     1741 acaattaagc aggtgcatcc caatgtgctt tcagttactg gcccacaggc gaccgaagaa
     1801 gtgatggaag caattcatat tcatttacca aaaccccatg atccttatgt cgatttggtt
     1861 cccccgcagg gaattcggct gacccctcaa cattatgctt atttgaagat ttctgaaggt
     1921 tgtaatcatc ggtgctcttt ttgcattatt ccaagtatgc ggggagatct cgtgagtcga
     1981 tcggttggag atatcatgca ggaggcacaa aatttggttg atgcaggggt taaggaattg
     2041 ctcgttattt cacaagacac cagtgcatat ggcgtggatt tgaaataccg taccggtttc
     2101 tggggaggaa aaccgattaa aacacggatt acagagcttg caactgcact aggtgagctc
     2161 ggtgtttggg tgcggcttca ttatgtttac ccttatccgc atgtagatga actaattcca
     2221 ttgatgagtg atcttaagat cattccttat ctcgatatcc cctttcagca tgcaaacgcc
     2281 cgaattctaa agctgatgaa acgccccgcc aatagtgaaa atgttctcgc acgaattcag
     2341 cagtggcgga cgatttgtcc agaaattacg ctgcggagca catttattgt cgggtttcct
     2401 ggtgaaacgg agcaagagtt tcaggaatta ctggattttc ttgaaatcgc ccagttggat
     2461 cgagttggcg cctttatgta ttctcccgtt gagggtgctg ctgcaaataa tttacctgat
     2521 catatctcgc ctgaaattca agaagaacgc ctggccagat tcatggcact acaagaaaaa
     2581 attagcgcgg ataagttaaa acaaaagata ggtaaaacgc tagaagtgct agttgatgaa
     2641 gtggatagcg aaggcgcgat tgcacggagt catgctgatg cgcctgagat cgatggatta
     2701 gtatatatcg atgcggatca aaaaacgata caacaaggcg agtttgtcaa agtacgcatt
     2761 gttaattccg atcagcatga tttgtgggca gagcttgttt aatagaattt taggctttag
     2821 tcgctttaac ttaggggaac aagtttggag ttgattttat ggagacatgc ggaggcagag
     2881 gatggggtac cagatgcttc gcggagattg actgaaaagg gtatgaaaca ggcccaatcc
     2941 atggcccaat ggttaaaacc tcgactgcca aagcatactc gtatcattgt tagcccagcg
     3001 aaacgaaccc agcaaacggt tgcagcgcta tgcgatacat atgaaacact tgaagcagtt
     3061 ggcacaagta ccacagcaga taaagtgctt gccactgtca attggcctta tgaagaaggc
     3121 gctgtaattg tggtgggaca tcaaccgaca ttagggcaaa ttgcagcagc aatattaggc
     3181 aacccttatg caagctttag tgtaaagaag ggttcgattt ggtggcttag ctcaaaagct
     3241 aaggatttat catcgaatgg aagtctacgg ttagttatgg tgcccgatat gatttagatt
     3301 aaaagtaaga agtaaaactg ttctatttct gaatgtgtga attttgtgtt aattaaggaa
     3361 atgactgaat ttatgaggtt tttttattat ttaaataatt acgaaatttc ttgactaacc
     3421 ggtcacatct cattagattg attacttaac ataagcccta gggaggataa gttttaatat
     3481 ggatatagat atagtttcgg ctttatctgg attaatcgat ttgccgtggt gggggtacat
     3541 tgtcgttaca ttggtactaa cgcatattac cattgcaagc attacaattt ttttacatcg
     3601 ccatcaagca catcgtgctt tggatttgca tccgataccc agtcattttt tccgtttctg
     3661 gctttggctg acgagcggaa tggtaaccaa agagtgggca gcgattcatc gtaagcatca
     3721 tgcaaaatgt gaaacgttag atgaccctca cagtccacaa atttatggaa ttaagaaggt
     3781 tttattagag ggttctgagc tttatcgcaa ggaaggaaag aacctagaaa cactggaaag
     3841 atatggtcat ggtacacccg atgactggat ggaacgtaat gtatatacca agcatagtgt
     3901 tgctggcgtg gcttcaatgt taatcattaa tgttgtctta tttggaccaa tcggaattac
     3961 gatatgggcg gttcaaatgc tttggacacc tgtaatggca gcaggcatta ttaatggtgt
     4021 tgggcattat tgggggtatc gtaattttcg tgctgaagat gcgagccgta atattgtacc
     4081 gtggggaatt ttgattggtg gagaggagtt gcataataac catcatgctt atccaacttc
     4141 tgcacgactt tctaataaat ggtttgaatt tgatattggt tggatgtata tccgtattct
     4201 tgagatgatg ggattagcaa aggttaagaa aattgcgcct aaattaaatc tcaatgaatc
     4261 taagacagaa tgtgattttg atacattaca agctgttatt gcacatcgtt atgaagtatt
     4321 ggctaagtat acgcaatcac tgaaagcaat ccttaaaaac gagatggaac aattgttgca
     4381 agttgcagcg catcatggtg ttgatggctt aacattgaaa cggtgggtac tagcggacgc
     4441 tagcactttg caagatgagg agcgtaataa gctgagtttg gtgctgagta aaacgaagca
     4501 acttgataaa gtttatacga tgcgtgaaga gttatctgca atctggcaaa gatcatctgc
     4561 ttctaaggat gagctagtga agcagctaga agattggtgc tatagagcag agcaaagcgg
     4621 tattgaggca cttgagaaat tctcgcgtag acttcgctgt tacgcataaa gtttgtatct
     4681 ttgctctcga gcaaattagc ttcgtgtaat ttgctcgaga tgtcctaaag ctagattgtt
     4741 actatttttt tagctccacg taagtcacac atcttttccc agttccgagt attccttaat
     4801 ttttgtattg tttcatcaaa gtaaagatag taaaaattga ttttcttacg ctataatagt
     4861 gcaactgtgt tgcattaatt gcattatgaa tttaattgaa agagcaaaag aattagtaag
     4921 tcttacaggg gatcgagcga ctgccaatcg agttagaacg ctttctattt tactggcagc
     4981 aaaacgggcg ctgacccatc gtgaaattgc ggatcaacta ctcgatgcaa agaaacaact
     5041 agatcgtgta acgctttatc gcatccttga gtggatgagt aaaaagagtt taatccataa
     5101 gatcgctggg gatgacagag tctggcgttt ccggattaat acagaaattc gtgcccacca
     5161 acatgcgcat tttaaatgta accgttgtgc aaaagtaatc tgtttaaaag attctaagta
     5221 taatcccacc cctttattac ccgtcgggta tttacttcaa gaaattgaat tgactattaa
     5281 gggagtgtgt tcggaatgta tttagtgatc aattagatca aattttccat gatttggttt
     5341 ttttatgcac accaataatg caactaagtt gcattattgg tgtgcgatgt tattaataaa
     5401 tttgattcat atgatgataa gaagaacgac aaacagcttt cgattaagtc gcaatatacc
     5461 gcagcgttgg ttaattttga gtctggtggg atcttgctgg ttgagtgccg ttgcttttgc
     5521 gcaagttgaa gacacgagaa aaatgatcag aatgcctgag ctcgttataa catcaacccc
     5581 atttaaagat cggagtgagc tcaacatgac gcaaccgacg agtatcttgc aaggtgataa
     5641 ccttcgtcgc aagcgtgaag caagtttggg agatacctta atcaacgaag ttggagtgac
     5701 ttccagttca tttggtccag gtgcaggtag gccgattatt cgtgcacttg atgggcctcg
     5761 gattcaagta ttagaaaatg gaattggaag tttggatgta tcttcgctga
//
LOCUS       DAOQKQ010000164         5665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_7486_length_5665_cov_5.669340, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000164 DAOQKQ010000000
VERSION     DAOQKQ010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7486_length_5665_cov_5.669340"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..257)
                     /locus_tag="PKZ49_10150"
     CDS             complement(123..257)
                     /locus_tag="PKZ49_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36967.1"
                     /translation="MSWLLCLLCNICFSDGVQNYAHARAQHAEHDHLVAPQTIDESHS
                     "
     gene            529..1965
                     /locus_tag="PKZ49_10155"
     CDS             529..1965
                     /locus_tag="PKZ49_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HND36968.1"
                     /translation="MQNSIGEQLVEDGLLTPSQLAQAYTWQQHNGRQLAYAITYLGFV
                     TQAQLTHYLNLELPVPQKIEDTGLSEALLTDLLLKSAYLESGIFSIQSITSSLSLPFS
                     VVNQLIELIRSDHLITVRGAASYTSSQPLELTQRGRERAEAALQISSYVGAAPVPIEV
                     YTHILNKQSVRLIELDREWVYSSLSHLVMSDQLINQLGPAFASGRSVFLYGQPGVGKT
                     SIAEALGNSLPGEVYIPQALEVGGQIIRFYDPVTHVSIESDQTEHVSQLDLSKKLTYD
                     PRWKKCRRPVIMVGGELTLDMLELKYDAVSKFYEAPIHMKASNGVFILDDFGRQRVDP
                     RQLLNRWIIPLEKGVDFPSLHTGLKFKIPFDQITVFSTNLKPTDLVDEAFLRRIRHKI
                     QIPSQTEGEYKEILRRACHTYGVHYDSSVVEYLLNNYYRKQNRTLAGSHPRDLLEHII
                     EYAAFLKQTPEFTIETVNAAAANYFVDL"
     gene            complement(1996..2964)
                     /gene="ispB"
                     /locus_tag="PKZ49_10160"
     CDS             complement(1996..2964)
                     /gene="ispB"
                     /locus_tag="PKZ49_10160"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HND36969.1"
                     /translation="MSIDSVRNFIAQDMSAVDEVIRQKLYSQVALIRQISEYIINNGG
                     KRLRPALVILSAGVFGYSGKYHHQLAAVVEFIHTATLLHDDVVDESELRRNNATANAL
                     FGNAASVLVGDFLYSRAFQMMVEVNSMRVMQVLADATNVIAEGEVLQLLNCRDPNVEE
                     DNYLRVIRYKTAKLFEAATRLGAILGNATEEEENAMAVYGMHLGTAFQLIDDLLDYSG
                     DHQDTGKNLGDDLAEGKPTLPLIYAMRTGSAQQANVIRKAIETGGESGFDSILTIIEE
                     TGALNYAKQRAYQEVEIAAKAISLLKSSHYKDCLLQLAEFAVTRNH"
     gene            3111..3422
                     /gene="rplU"
                     /locus_tag="PKZ49_10165"
     CDS             3111..3422
                     /gene="rplU"
                     /locus_tag="PKZ49_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HND36970.1"
                     /translation="MYAVIKTGGKQYRVEVGNKLKIEQIKADAGSDIVLDQVLMVANG
                     DDVTVGKPYLEKAKVNASVLCHGRHDKIRIFKMRRRKHYQKHQGHRQNYTEIQITNIS
                     A"
     gene            3439..3696
                     /gene="rpmA"
                     /locus_tag="PKZ49_10170"
     CDS             3439..3696
                     /gene="rpmA"
                     /locus_tag="PKZ49_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HND36971.1"
                     /translation="MAHKKAGGSSRNGRDSHSKRLGVKRYGGELIKAGNIIVRQRGTQ
                     FHPGDNVGMGKDHTLFAKIEGKVLFSMKSSFNRRTVSIVPV"
     gene            3837..4865
                     /gene="obgE"
                     /locus_tag="PKZ49_10175"
     CDS             3837..4865
                     /gene="obgE"
                     /locus_tag="PKZ49_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HND36972.1"
                     /translation="MKFIDEATILVSAGNGGDGVASFRREKFIPKGGPDGGDGGHGGS
                     IYAIADHNINTLIEYRYTPAFRAKKGENGRGSDCYGKGAEDIVLRMPVGTVITNAETG
                     EVIADLKHHLQKELLAKGGKGGLGNLHFKSSTNRAPRQFTRGGIGDEFQLKLELKVLA
                     DVGLLGMPNAGKSTLIRAVSAARPKVADYPFTTLHPNLGMVRVDHHRSFVMADIPGLV
                     EGAADGVGLGHHFLKHLERTRLLLHVVDIAPMNDTIDPVQEAKALVEELRKYNEALYH
                     KPRWIVFNKIDLLPASEQEAACEQFIRDLKWKDKWFSISALTGEGCKSLTYAVMDYLE
                     QTFSAMQH"
     gene            4868..>5665
                     /gene="proB"
                     /locus_tag="PKZ49_10180"
     CDS             4868..>5665
                     /gene="proB"
                     /locus_tag="PKZ49_10180"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HND36973.1"
                     /translation="MNQAVLSAARRIVIKVGSSLVTNQGQGLDHQALAGWAKQISALK
                     QLGKEIILVSSGAIAEGMQRLSWKKRPTAIYELQAAAAVGQMGLAQAYADCFSQYDLL
                     TAQILLTHDDLSNRKRYLNARSTLSTLLKLNIIPVINENDTVVIDEIRFGDNDTLAAL
                     VTNLVEAEVLVILTDQSGLFTSDPRKNDNATLVQEANAGDPEIEAMAGGAGSEIGRGG
                     MQSKVIAAKRAARSGAHTVIASGHEREVLVRLAKGEAIGTCLLANTPV"
BASE COUNT         1661 a         1091 c         1318 g         1595 t
ORIGIN      
        1 cctattgatc ttgctatata agatgaaaaa cactgagaat agttcatagg cattaataaa
       61 aataagttat aaagaattac cagacggtgc tgagattaca tattctacag aaatccaaaa
      121 acttaagaat gcgattcatc aatggtttga ggcgcaacta agtgatcatg ctcagcatgc
      181 tgtgcccggg catgagcata attttgaaca ccatcactaa agcagatatt gcagagcaag
      241 caaagtaacc aactcataga tttgaagaag cttactgaaa tatccaagat tgaatagtag
      301 ctctaaacct gagtttcttc taatcaagaa tcaaagaagc tccctaatta ccaacctatt
      361 ccttatttta cagaattaaa caacagtgta gattcattcg tatattctta ctctatgtat
      421 ccgtgccaat ggactagcag catttgaagt aagagcgtta cgaatgatct ttaggtgaac
      481 caagttttat ctgtaagcaa ataaattttt aaggatagat atttaacaat gcaaaattca
      541 attggtgaac agttggttga ggatggatta ctaacgcctt cacagctagc gcaggcttat
      601 acgtggcaac agcataatgg aaggcaattg gcttatgcaa tcacgtattt gggttttgta
      661 actcaggcac aattaactca ctatctgaat ttagaattac cggtaccgca aaaaatagag
      721 gatacgggtc tctcagaggc tcttttgacg gacctgttgt taaaatccgc ttacttagaa
      781 tcaggcattt tttctataca aagtatcaca agttctttat cgcttccttt tagcgtagtt
      841 aatcagctga tcgaactgat tcgatccgat catttaatta ctgtacgggg agcagcgagc
      901 tatacgagtt cacaaccgct cgaattgacc caacgcggca gggaaagagc tgaagcagcc
      961 ttgcaaatta gttcttatgt gggtgcggca cctgtgccaa tcgaagtata cacgcatata
     1021 ttgaataaac agtcagtgcg cctaattgaa ttagatcgtg agtgggttta ttcatcattg
     1081 tcacatttgg taatgagcga tcagttaatc aatcaattgg gtcccgcttt tgcatcagga
     1141 cgctctgtat ttttatacgg gcaaccgggt gtaggaaaaa ccagtattgc ggaggcattg
     1201 ggtaatagtt tacccggtga ggtttatatt cctcaggcgc tagaagtagg cgggcagatc
     1261 atacgatttt atgaccctgt gactcatgtc agtatcgaat ctgatcaaac ggaacacgtg
     1321 tcacagttag atttgagtaa gaagttaact tatgatccgc gctggaaaaa gtgtcgtaga
     1381 ccagtcatta tggtgggagg tgaactcacc ttagatatgc ttgaattaaa atacgatgcg
     1441 gtgagtaaat tttacgaagc acctattcat atgaaggcga gtaatggtgt tttcatcctc
     1501 gatgacttcg gtcggcagcg tgtcgatccc cgtcagttat taaaccgctg gattattccg
     1561 ctggaaaaag gggttgattt tccatcgcta cataccggat tgaaatttaa aatccctttt
     1621 gatcaaatta ctgttttttc aacgaatctc aaaccaacag atttggtgga tgaagcattt
     1681 ctccgtcgaa ttcgccataa gattcagata cccagtcaaa cggaagggga atataaagag
     1741 atattgcgtc gagcgtgcca tacttatggc gttcattacg atagtagcgt ggtggagtac
     1801 ctattgaata attattatcg taagcaaaat cgtacgctag ccggaagcca tccgcgtgat
     1861 ttgctcgaac atattattga atacgctgca ttcttgaaac aaacaccaga atttaccatt
     1921 gaaacagtta atgcagctgc agctaattac tttgtcgatc tataaataat cgaactggga
     1981 ttgcagtaac cgtttctaat ggttgcgagt taccgcaaac tcagcgagct gtaataggca
     2041 atctttataa tgagagcttt ttaacagaga tatcgctttg gccgcaattt cgacttcctg
     2101 gtaagcacgt tgtttagcgt agttaagtgc ccccgtttct tcgataatcg ttaggataga
     2161 atcaaaccca ctttctcccc cggtttcaat tgctttacga atgacgtttg cttgttgagc
     2221 tgagccagtt cgcatcgcat aaatgagggg taatgtcggc ttaccctcag cgagatcatc
     2281 tcctaaattt ttgcccgtat cttgatgatc ccccgaataa tcgagcaagt catcgatgag
     2341 ttggaatgcc gtaccgagat gcatcccata aacggccatg gcattttctt cttcttccgt
     2401 agcattacca agaattgcac ctaagcgtgt cgctgcctca aaaagtttgg ctgttttata
     2461 gcgaataacc cgtaggtaat tatcttcttc tacattggga tctcggcaat tgagcagttg
     2521 taatacctca ccttcggcaa tgacattggt cgcatcagca agaacttgca taacacgcat
     2581 actgttgact tctaccatca tttggaaagc acgtgaatag aggaaatcac caaccaatac
     2641 gctggctgca ttaccaaata atgcattggc tgttgcattg tttcgtctca gttccgattc
     2701 atcaacaaca tcgtcgtgca ataaagttgc ggtatgaatg aattcaacaa cggcagcaag
     2761 ctgatgatgg tattttccac tataaccgaa tacgcccgca gataagatga ctaatgctgg
     2821 gcgtaaacgt ttaccgccat tgttaataat atactcactg atttgtcgaa tcagcgcaac
     2881 ttgagaatag agtttttggc gtattacttc atccaccgcg ctcatatctt gagcgataaa
     2941 atttcttaca gaatctatag acacaacttc cccttgtaga aaacactatg gtaggaatgt
     3001 ctttactaga gtgtcaagtc ggatcattcg tttaagattg tttttttatc acacaaaacg
     3061 cttataggaa ggtataattc atgccaatta gtggaaaggg tatgaacgat atgtatgcag
     3121 tgattaaaac aggcggtaag caataccgcg ttgaagtcgg taataagctt aaaatcgagc
     3181 aaattaaagc agatgccgga agtgatatcg tgcttgatca agtgctcatg gttgcaaatg
     3241 gagatgatgt cacggttggt aaaccttacc ttgaaaaagc gaaggtaaat gcttctgttc
     3301 tatgtcatgg tcgccatgac aagattcgca tctttaaaat gcggcgacgc aagcactatc
     3361 aaaagcacca agggcatcgg caaaattata ctgaaattca aattaccaat atttcagcat
     3421 aaagatagga gaatttagat ggcacataag aaagcgggcg gaagctcaag aaacggacgc
     3481 gattcacact ccaagcgcct aggggttaag cgctatggcg gcgagctaat taaagcggga
     3541 aatattatcg ttcgtcagcg tggaacgcaa tttcatcctg gtgataatgt gggaatgggt
     3601 aaagaccata cattattcgc aaaaattgaa gggaaagtgc tctttagcat gaaaagttca
     3661 tttaaccgta ggactgtatc gatagttccc gtttaactga tagaaatgaa taaaaagccc
     3721 cattcaagtg atggggcttt tttatttgta acttgatttt ataattagtt gttactgaaa
     3781 tttgcaaatt tgtttcctgt ttttcttgtg cattaatttt tctactacga tagtttatga
     3841 agtttattga tgaggcaact atattggttt ccgccggtaa tggaggggat ggtgttgcga
     3901 gctttcgtcg tgaaaaattt attccgaaag ggggtcctga tgggggggat ggcggtcatg
     3961 gcggaagtat ctatgccatt gcagatcata atataaacac tttgattgaa tatcgttaca
     4021 cacctgcttt tcgtgccaag aagggtgaaa atgggcgtgg ttcggattgt tatggaaaag
     4081 gggctgaaga cattgtgttg cgcatgcccg ttggaactgt gatcacaaat gccgagacgg
     4141 gagaagtcat agcagattta aaacatcacc tgcaaaaaga attattggcg aaagggggta
     4201 aaggagggct cggtaacttg cattttaaat caagtaccaa tcgtgcgccc agacaattca
     4261 cccgaggggg aattggggat gaatttcagc ttaagcttga gttgaaggta cttgccgatg
     4321 tgggtttatt gggaatgcca aatgcgggta aatctacttt gattcgtgct gtgtcggcag
     4381 ctcgaccaaa agttgctgac tatcctttta cgacgctgca tcctaatttg ggcatggtca
     4441 gggtcgatca tcatcgtagc tttgtcatgg ctgatattcc aggtttggtt gagggcgctg
     4501 ctgatggggt agggcttggc catcattttc ttaaacattt agaacgaacc cgactgcttc
     4561 ttcatgtggt cgatatcgca ccgatgaatg atacgatcga tcctgttcaa gaggcgaaag
     4621 cgctagtcga agaactcagg aaatataatg aagctttata tcataagcct cgctggatcg
     4681 tgtttaataa aattgactta ttgccagcct cggaacaaga agcggcttgt gagcaattca
     4741 ttcgtgatct gaaatggaaa gataaatggt tcagtatttc agccttaaca ggggaggggt
     4801 gcaagtcctt gacttatgct gtcatggatt atttggaaca aacatttagt gcaatgcaac
     4861 actgatcatg aatcaagctg tacttagcgc tgcacgtcgc attgtgatca aagtgggaag
     4921 tagtttagta acgaatcagg ggcaaggact cgatcatcaa gcccttgcag gttgggcaaa
     4981 gcaaatttct gcgctaaagc agttagggaa agaaatcatt ctggtttctt cgggtgccat
     5041 tgcagaggga atgcaaagac tcagttggaa gaaacgtcct accgcaatct atgagctgca
     5101 ggccgcagcg gcggttggtc aaatgggtct ggcgcaggcg tacgcagact gttttagtca
     5161 atacgatttg cttacagcgc aaatattatt gacacatgat gatttatcga accggaaacg
     5221 ctatcttaat gcccggtcta cactgagcac gctattaaag ttaaatatta ttccagtcat
     5281 caatgagaac gataccgtgg tcatcgatga aatacgattt ggcgataacg atacgctggc
     5341 tgcattggta accaatctcg tagaggcaga ggttctggtt attttgaccg atcaatcggg
     5401 cttatttacc agtgaccctc gcaagaatga taacgcaacc cttgttcagg aggccaatgc
     5461 gggagatcct gaaatcgagg caatggccgg tggtgcaggg agtgaaatcg ggcgaggtgg
     5521 aatgcagagt aaagtcattg ccgctaaacg ggctgccaga agtggggcgc atacagtgat
     5581 tgcatcgggg cacgagcgtg aggttctagt tcgtttagca aaaggtgaag cgattgggac
     5641 ctgtttgcta gctaatacac ccgtg
//
LOCUS       DAOQKQ010000165         5613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_7596_length_5613_cov_6.358942, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000165 DAOQKQ010000000
VERSION     DAOQKQ010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7596_length_5613_cov_6.358942"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..1650)
                     /gene="argA"
                     /locus_tag="PKZ49_10185"
     CDS             complement(211..1650)
                     /gene="argA"
                     /locus_tag="PKZ49_10185"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HND36974.1"
                     /translation="MKIFGQNEIAEIDLPITRMKKNFTPPSISVDLKNEDLHTYFIAW
                     FRSVAPYIHTFRGKIFVVAFGGEVVADGKFVELVHDLNLLASLGIQLVLVHGARPQIE
                     SRLQRDNLDITYVNGIRATDAVTLERVIESVGRVRMEIEALMSMGLPNSPMANASIRV
                     TSGNFVIARPIGVMNGTDLMYTGEVRKINLTAIHSHLTQGEIVLISPLGYSPTGEIFN
                     LTLENVAAEVAITLKAEKLIFLMNTPGIMDQSTKQPDNLLRELTVAESKSYLTQLADL
                     PTHIPDDTQFYLPQAIHACENGVGRAHLISRHVDGALLQELFSHDGIGTMITRDSLQS
                     IRKAKIEDVASIIQLIEPLEMEGILVRRNRELLEMEIDRFTVLEHDNIIIACAALYPF
                     PEEQAYELACLAVHARYRKTGCGSALLKHLENQIKSKKPQKLFVLTTRTAHWFVERGF
                     VETSLEALPKRKKQLYNYQRRSKVFTKII"
     gene            1878..3443
                     /locus_tag="PKZ49_10190"
     CDS             1878..3443
                     /locus_tag="PKZ49_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HND36975.1"
                     /translation="MIFWRVKSLDTILATAEKKSLNRTLGVWQLTLLGVGAIIGTGIF
                     VLTAEAAQKAGPGMMFAFIIAGFVCAVAALCYSELSSMVPVSGSAYTYSYAVLGEFVA
                     WIVGWGLILEYSVAAGAVAVGWSGYFVGLLSNSLGIEIPFMLANGPFAGGIINLPAVV
                     ICMLVIGLLVVGTKESATFNAVLVAVKITALTIFIALALPVSDMENFQPFLPLGSTGV
                     VAAAASIFFAYVGFDAVSTAAEETKNPQRNVPLALIGSLAICTIFYLFISAGVIGSVG
                     AQPVMDTAGKAFSPGSLALAEQCQALASMGQHPVVCSREALAHVLRQIGWEKIGNLLG
                     LAAFLALPSVILMMLFGQTRIFFVMARDGLLPEVLSRIHSRFKTPHIVTLVTGVGVTL
                     AAAFLPVGKLADISNSGTLFAFMVVAITVMILRVRDGNRARPFRTPAIWFVGPLAVIG
                     CITLFLFLPADAKLVFPIWTGIGLVFYFLYGYRKSHVALGTCASAGGENIISPLRSLA
                     DGDDKNTNPNNNH"
     gene            3467..4408
                     /locus_tag="PKZ49_10195"
     CDS             3467..4408
                     /locus_tag="PKZ49_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HND36976.1"
                     /translation="MRTAYITHPICLKHDMGYGHPESPLRLQAIEQELRVEGLFPLLR
                     YYEAPLVTHEQLARVHAEHYLHAIETVAPQHGLISIDSDTLMNPYTLEAAKRAAGAAV
                     LATDLVISGQVENAFCNVRPPGHHATRDEAMGFCFFNNVAVGAAHALALHGLTRIAIL
                     DFDVHHGNGTETIFKDEPRIMLCSTFQHPFYPYSGANSSSDHIINTPLAAGTTGQGFR
                     ATVLENWIPALERFKPEMIYISAGFDAHREDEMANLNLVEADYIWVTEQIMTIAERYA
                     QKRIISALEGGYELDALARSVVAHIRVLSAIAEYNSD"
     gene            4572..4647
                     /locus_tag="PKZ49_10200"
     tRNA            4572..4647
                     /locus_tag="PKZ49_10200"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4605..4607,aa:Thr,seq:tgt)
     gene            complement(4682..>5613)
                     /locus_tag="PKZ49_10205"
     CDS             complement(4682..>5613)
                     /locus_tag="PKZ49_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HND36977.1"
                     /translation="DKTQAQEFHDKLKAESWRVARLGDKPKRTWDEAAYKWLMETQHK
                     ASHKQDMKQVAWFQQFFRGQLLSELTREVIAEVGEIKCKETSPSTANRYLATIRAILR
                     RAALDWEWIEKAPFIRLYKEPKRRIRWIAPEQVQILLNELPEHLVDLVKFSLSTGLRK
                     RNVTELEWSQVDLQRNVAWIHADQAKARKSIHISLNATAMGVLRKQVGKHSLRVFTYQ
                     GKPIIQVNTRAWRKALRRAGIEDFRWHDLRHTWASWLTQKGVPLNVIQEMGAWESTEM
                     VKRYAHLAPEQFRKHAMVVDELLNDTNLTQVEN"
BASE COUNT         1404 a         1162 c         1300 g         1747 t
ORIGIN      
        1 ggattgaaac ccagcggtcg tatttattgt ttgcgatgtt gtactagtag cgcccagcat
       61 ccaagctggg cgaggattga aacatgggct tgtcttatat tcatatatgc acaaaatagt
      121 agcgcccggc gtcgaagccg ggtgaagatt gaaattgtcg attattcaaa aaagggacat
      181 gtgttttata gcaatctgat atagaaagta ctaaataatt ttcgtaaaga ccttagaacg
      241 tctttggtaa ttgtaaagtt gcttttttct ttttggcaaa gcttccaagg atgtttcgac
      301 aaatcctcgt tccacaaacc aatgtgcagt tcgtgtggtg agtacaaaaa gtttttgtgg
      361 tttttttgat ttgatctgat tttccagatg ttttaacaaa gcgcttccac agcctgtttt
      421 ccgatatcga gcatggacag ccaagcaagc aagctcatag gcttgttctt cagggaaggg
      481 gtaaagcgca gcacaggcga tgataatatt atcgtgctct aataccgtaa aacggtcgat
      541 ttccatttcc agtaattccc ggtttcttcg cacgagaatg ccttccattt cgagtggctc
      601 gataagctgg ataatacttg cgacatcctc gatttttgct tttcgaatgc tctgtaggga
      661 atcacgggta atcatggtgc caatgccatc atgactgaat aactcttgaa ggagggcacc
      721 gtcaacatgg cggctgatga gatgagcgcg tccaacgcca ttttcacagg catgaatggc
      781 ttgtggtaaa tagaactgag tatcatccgg aatatgggtg ggaaggtcag ccaattgggt
      841 tagataggat ttgctttctg ctaccgttag ctcgcgtaat agattatctg gctgctttgt
      901 ggactgatcc ataatgcctg gcgtattcat caaaaaaatc aatttctctg cttttagcgt
      961 gatggcgact tctgccgcaa cattttcaag cgttagatta aaaatttctc cggtaggtga
     1021 ataaccgagt ggtgaaatta aaacaatttc cccttgagtt aggtgagaat ggatagcggt
     1081 taaatttatt ttccggactt cgccggtata catcaagtca gtcccattca tcacgccaat
     1141 cggtcgagca attacaaaat taccgctggt aactcggata gaagcgtttg ccatgggaga
     1201 attagggaga cccatggaca ttaaagcttc aatttccatg cgtacacgtc ctactgattc
     1261 aatgacacgt tcaagcgtta cagcatcggt agcgcggata ccattaacgt aggtaatatc
     1321 aagattatct cgctgcaatc ttgattcgat ctgggggcgc gcaccatgta caagcacaag
     1381 ctggattccc aggcttgcca ataaatttag atcatgcacc agctcgacaa attttccatc
     1441 cgcaaccact tctccgccga aggcaaccac aaaaatttta ccgcggaaag tgtggatgta
     1501 aggtgctact gaacggaacc aggcaatgaa ataggtgtgc aaatcttcat tttttagatc
     1561 gactgatatt gagggagggg tgaaattttt tttcatgcgt gtaatgggta aatcaatttc
     1621 agcgatttca ttctgaccaa agattttcaa aaaaacggct ggatttagct aagttaagta
     1681 tatcgactaa gttagctagg aagtatttca caagctgcaa gattattcaa acgtgcttga
     1741 gggtagtcac gaatcgaaaa tgaatcgatt agttgcgttg cagactattt ttttggagct
     1801 tgctcgccac ttatttcttg taaactattc cctaaatgaa tgttgaataa tgattgttgg
     1861 aattaaaggg gaaatcgatg attttctggc gagtaaaatc actagatacg atcttggcga
     1921 cagcagaaaa aaaatcgctc aatcggacgc tgggtgtgtg gcaactaaca cttttgggtg
     1981 tcggtgctat tattggcact gggatatttg tattaaccgc tgaggcggct cagaaagcag
     2041 gccctggcat gatgtttgcg tttatcattg caggtttcgt gtgtgcagtt gcggcattat
     2101 gctattcaga gctttcttcg atggtaccgg tttcgggttc tgcttatacc tactcttatg
     2161 ctgtactcgg tgagtttgtt gcgtggattg tcggctgggg gttaattctt gaatactcag
     2221 ttgcggctgg cgctgttgcc gtagggtggt cagggtattt cgttgggttg ctgagcaact
     2281 cattagggat tgagattcca ttcatgctgg caaatggacc ttttgcgggt gggattatta
     2341 atttgccagc cgtggtgatt tgtatgctgg tgatcggttt gctggtcgtc ggtactaaag
     2401 agagtgctac ctttaatgca gtgttggttg cagtcaagat taccgcgctc acgatattca
     2461 ttgcgcttgc cttgccagtt tccgatatgg aaaattttca accatttctt cccttggggt
     2521 cgacgggtgt cgttgccgca gccgcgtcta tcttctttgc ttacgtaggg tttgatgcag
     2581 tatcaaccgc agcggaagaa acaaagaatc cacagcgcaa tgttccactt gcattgatcg
     2641 gatcgcttgc gatttgtacg attttttatt tatttatttc tgccggtgta attggctcag
     2701 tcggtgcgca accggtgatg gataccgctg gtaaagcttt ttcccctggt tcgcttgcgt
     2761 tagcagagca atgccaggca cttgcgtcaa tggggcagca tcctgtggtt tgctcacgtg
     2821 aagcgcttgc gcatgtatta agacagatcg gttgggaaaa aatcggtaac ttacttggtt
     2881 tggccgcgtt tcttgcatta ccttcggtga tcttgatgat gctatttggg cagactcgga
     2941 tttttttcgt gatggctcgc gatggtctat tacctgaagt gctgagccga attcattctc
     3001 gatttaagac accccatatc gtgacattgg ttacgggggt tggtgtgacg ctagcagcgg
     3061 ctttcttacc ggtgggtaag ctcgctgata tttccaactc tggtacgctc tttgctttta
     3121 tggttgtagc gataactgtc atgattttgc gtgtcagaga cgggaaccgt gcaagacctt
     3181 tcagaacacc tgcaatatgg tttgttggcc cacttgctgt cattggttgc attacccttt
     3241 tcttattctt accggctgat gctaagcttg tatttccaat ttggactgga attggtttgg
     3301 ttttttactt tctttatggt tatcgaaaaa gccatgtggc gttgggtact tgtgcttctg
     3361 ctggaggaga aaatatcatt agccctttac gatcgctcgc ggatggtgac gataagaaca
     3421 ccaatccaaa taataatcac taattaatgg gtaaaccgct gcgtacatga ggactgctta
     3481 cattactcat ccgatctgtt tgaagcatga tatgggttat ggccatcctg aatcgcctct
     3541 gcgattgcaa gcgatcgagc aggagttacg cgtagaaggt ctttttcctt tattgcgata
     3601 ctatgaagcg ccactggtga cccatgagca gttagcccgt gttcatgcag aacattatct
     3661 tcacgcgatt gagactgttg caccgcagca tggattgatt agtatcgatt ccgatacttt
     3721 gatgaatcct tatacccttg aggcagccaa gcgagcagcc ggcgccgctg ttttagcaac
     3781 ggatctggtg atatccggtc aggtagaaaa tgcattttgt aatgttcgac ctccaggtca
     3841 tcatgcaacc cgtgatgagg caatgggttt ctgctttttt aacaatgttg cagtgggtgc
     3901 tgcgcatgct ttggctttgc atgggctgac tcgaattgct attttagatt ttgatgtgca
     3961 tcatggaaac ggtactgaaa ctatatttaa ggatgagcca cggataatgc tttgttccac
     4021 attccaacat cctttctatc cttatagcgg tgcaaatagc agcagcgatc atatcatcaa
     4081 tacacccctt gctgctggta caacaggtca agggtttcgt gcaacggtac tagagaactg
     4141 gattccagca ttagaaagat ttaaacccga gatgatttat atctctgcag gttttgatgc
     4201 gcatcgcgaa gatgaaatgg ctaatctaaa cttggtggaa gcggattata tttgggttac
     4261 cgagcaaatt atgactatcg cagaaagata tgcacaaaaa cgaattattt cagcgttgga
     4321 aggagggtat gagctggatg cattggccag gagtgtggta gctcatatcc gagtactcag
     4381 tgctattgcc gaatacaatt cagattaatt ctttttattg agtcgcttat ttatttgtgc
     4441 ctgatattcg ggtagaatgc ttgcgcgaat taagcggttt atcgctaata gtattaaaaa
     4501 gctagaaccc tagcttctaa aagtagtcga gtgatagttg ttcacgcagc ctgttttaat
     4561 ttacttccat agccggcata gctcagttgg tagagcagct gatttgtaat cagaaggtcc
     4621 cgagttcgat tcttggtgtc ggcaccataa aatcaatgag ttagattgat tttatcatct
     4681 tctaattttc cacttgtgtc aaatttgtgt cattcagaag ttcatctaca accattgcat
     4741 gttttctgaa ttgttcgggc gccaaatgag cataacgttt caccatttca gttgactccc
     4801 aagctcccat ttcttgaatt acatttagcg gtacaccctt ctgagttaac caactggccc
     4861 atgtatggcg caaatcatgc caacggaaat cttctattcc tgctcttctt aatgctttcc
     4921 gccatgccct ggtattgacc tgaataattg gttttccttg gtaagtaaac actcttaacg
     4981 aatgtttgcc aacctgcttt ctcagaactc ccatagccgt tgcattcagt gaaatatgaa
     5041 tgctttttct tgctttggct tgatctgcat gaatccatgc aacatttctc tgcaaatcaa
     5101 cttgcgacca ttccaattca gtaacattgc gtttgcgcaa tcctgttgaa agtgaaaact
     5161 ttaccaagtc aaccaaatgc tctggtaatt catttaataa tatttgtact tgctcaggag
     5221 ctatccagcg aatccgccgc ttgggttcct tatatagccg gataaagggt gctttctcaa
     5281 tccattccca atctaaagcc gctcttcgta gaatggccct aatagtagcc aaataacgat
     5341 tagccgtcga aggtgaagtt tctttacatt ttatttcacc tacttcagca atcacttccc
     5401 gagttaattc agaaagtaat tggcccctaa aaaactgttg aaaccacgca acttgcttca
     5461 tgtcctgctt atgtgaagcc ttatgttgtg tctccatcag ccatttataa gccgcttcat
     5521 cccaagtccg ctttggctta tcccccagtc ttgcaacacg ccaggattcc gctttcagct
     5581 tatcgtggaa ttcttgcgcc tgggttttat ctt
//
LOCUS       DAOQKQ010000166         5546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_7754_length_5546_cov_5.542160, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000166 DAOQKQ010000000
VERSION     DAOQKQ010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5546
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7754_length_5546_cov_5.542160"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..522
                     /gene="lptE"
                     /locus_tag="PKZ49_10210"
     CDS             40..522
                     /gene="lptE"
                     /locus_tag="PKZ49_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HND36978.1"
                     /translation="MFGIVLALSACGFQLRGLVSNLPFETIHVTAPPGNPIGIDVERA
                     IRAGTDTKVVGSPENAQAILQIISTNNEKKILSLSAGGRVREFQLGYRVAIRLVDPQG
                     TEIAPIDEINLTRVLPFLDAQLLAKQAEEEMLYKDMQIDAVQQIIRRLASIKTTPKNN
                     "
     gene            complement(543..1367)
                     /locus_tag="PKZ49_10215"
     CDS             complement(543..1367)
                     /locus_tag="PKZ49_10215"
                     /inference="COORDINATES: protein motif:HMM:NF018420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HND36979.1"
                     /translation="MRKQRIASFFMLLILLVSYKQLFAIPVSLELALLVDVSRSVDAS
                     EYAQQKNGYVNAFKDPVIQDAIASFAAIGGIAVTYIEWSGANQQSVSVGWTQITNAAS
                     ANAFADAIDNVDRAFGLGGGTAPGSAIQFVTPKFSDNGFEGNRWVIDVSSDGIRNTGT
                     QASVARDHFLDLETVGEGLTKTINGLPIDNTDLGFLGLADWFQDNIVGGPNAFLIAAD
                     GGFDSFSDAVKTKIGREISEPPTSVVFEPTSLSLILIGLIGFYFVAYQPRRIRVQA"
     gene            1588..2604
                     /gene="holA"
                     /locus_tag="PKZ49_10220"
     CDS             1588..2604
                     /gene="holA"
                     /locus_tag="PKZ49_10220"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HND36980.1"
                     /translation="MRIKPEQLPQLLKKQQNQCFTIMGDEPLLSIEAADTIRHSLYYQ
                     GVTEREIFTVDHRFEWSKITQWSDQCSLFSTSKLLDLRIPTGKVGNDGNTALVTFLDN
                     LSPDLWFMATLPTIDKKSQASKWFTALDNRGVIVAIDSIERTQLPQWIQQRFHAQKQT
                     IAPDALQLLVDNVEGNLLAAQQEILKLGLLYPEATLSVEQVRNAVFDVARHDVFDLPE
                     AMLTGDIVRFTHMLENLHSEGAAPPLVLSILVDPLRKLLLLHRGVSEGKSLGYLFKEI
                     RVWGQQQTLLSSAMKRYAPPLLMEGLTHAALIDKMIKGIKQGEIWDEFLQYGLRFAER
                     SQTK"
     gene            2659..3075
                     /locus_tag="PKZ49_10225"
     CDS             2659..3075
                     /locus_tag="PKZ49_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HND36981.1"
                     /translation="MKAKIKWQEGVSFSGETESGHTLIMDGAPEAGGRNLGPRPMEMI
                     LLGLGGCTAFDVVMILQKGRHSVTDCFIEIDAKRATEDPKVFTQIHLHFIVSGKNLAA
                     HHVERAIDLSAEKYCSASIMLRSTANITHDFEIIDV"
     gene            3180..3425
                     /locus_tag="PKZ49_10230"
     CDS             3180..3425
                     /locus_tag="PKZ49_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36982.1"
                     /translation="MNNDNNDDHKSDESLHENMRYFLFWMIEQQELLHQKNVKKAQCF
                     IALIRTLHFVKTQPCSSNTHKVSTQFIARKLNQLSCH"
     gene            3512..4285
                     /locus_tag="PKZ49_10235"
     CDS             3512..4285
                     /locus_tag="PKZ49_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HND36983.1"
                     /translation="MEMITERIGNELAKFLSKPVRQSIQVAMISQEQLLATLQPADVL
                     LVEGNTRISTAIKYLTQSSWSHAALYIGNALPNDSPWNLPQVLVEADLKEGIRAVTIA
                     QYAQMHTRICRPIGLNDDDRKHLIDYAITRIGQQYDLKNIFDLARYLFPAPPIPTRFR
                     RRLLALGSGDPTRAICSTLIAQAFMSIHYPILPEVQRTWSDNPMHADCHAEIYHIRHH
                     SLYTPRDFDISPFFEIIKPTTRDHFDYHALTWDSETDNT"
     gene            complement(4333..4629)
                     /locus_tag="PKZ49_10240"
     CDS             complement(4333..4629)
                     /locus_tag="PKZ49_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36984.1"
                     /translation="MLFPDNLGVITCRCVLEAKKPVLFVSHAGGDWQMYCDDANHDFE
                     DEEAMRSELVVVHVAHLLAQDATLNDIADLPVDMGAERSQVGGSWVRFENADEE"
     gene            complement(4735..5331)
                     /locus_tag="PKZ49_10245"
     CDS             complement(4735..5331)
                     /locus_tag="PKZ49_10245"
                     /inference="COORDINATES: protein motif:HMM:NF014754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HND36985.1"
                     /translation="MDRLGQEELGRLDAVAGDGDHGIGMLRGVSAALDAAKKAHDAQA
                     GAGTLLTLSGEAWAARAGGTSGALWGVILNTFGVKLGDTTAPDTATVAGSVAAALCEV
                     MQRGKAQPGDKTLVDVLQPFSAALDQASARGISVVTAWQDAADIANTAAQQTRNLLPR
                     IGRARPHAEKSIGTPDPGAISLAMIITVASEVIRKHCP"
     gene            complement(5253..>5546)
                     /locus_tag="PKZ49_10250"
     CDS             complement(5253..>5546)
                     /locus_tag="PKZ49_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36986.1"
                     /translation="KAGVEKPRKTAQNTFNFKLSEDGSRAITTSPHMTDELFDVLLTN
                     FATGETKVLDQYVIQPVHLLSKDGTKAAWIVSDKKNSGVWMLWRVMVIMVSVC"
BASE COUNT         1619 a         1347 c         1111 g         1469 t
ORIGIN      
        1 agaaggtaca accgcatgat cgtttctcgt atatttatta tgttcggaat agtactcgcg
       61 ctctcagcct gtggatttca gttgcgagga cttgtatcca atcttccctt tgaaacgatt
      121 catgtgacag cgcctccagg taacccgatt ggaatcgatg tggaacgcgc gatccgtgct
      181 ggaaccgata ccaaagttgt tggatccccc gaaaatgcgc aagctatcct tcagatcatc
      241 agcaccaata atgaaaaaaa gatactttca ctttctgcgg gagggagggt aagagaattc
      301 cagcttggct atcgcgttgc tattcggctg gttgatccac aaggtactga aattgcccca
      361 atcgatgaaa ttaacttgac acgcgtactg ccttttctcg atgcacaatt acttgcgaaa
      421 caagctgagg aagagatgct ttataaagat atgcaaatcg atgcagtgca gcaaatcatc
      481 cgccgacttg cttctatcaa aactacacct aaaaataact agcgcattct cttcctggtt
      541 aattaggcct gaactcgtat tcttctcggt tgataagcta caaaatagaa gccaattaaa
      601 ccaatcagaa tcaaggacag gctcgtaggc tcgaaaacga cactcgtcgg cggttcactg
      661 atttccctcc caatttttgt ctttactgca tcgctgaagc tatcaaaccc tccatccgca
      721 gcaatcaaga acgcattagg gccacccaca atattatcct ggaaccaatc cgccaagcct
      781 aagaaaccca agtctgtgtt atcaatgggc aaaccattta tcgttttagt taacccttcc
      841 cctactgttt ctagatcaag aaagtgatca cgggctaccg aagcttgcgt tcccgtattt
      901 cggataccat cactggatac atcgattacc caacgattcc cttcaaaacc attatcacta
      961 aatttaggcg taacaaactg gattgcgctg cctggagctg tccctccgcc taacccaaaa
     1021 gcacgatcga cattatcgat cgcatcagca aatgcattgg cgcttgccgc gttagtaatt
     1081 tgtgtccaac cgacgctaac cgattgctga ttggcaccag accattcgat ataagtcacc
     1141 gcaatgccac ctatcgctgc aaatgaagca atcgcatcct gaataaccgg atctttgaat
     1201 gcgttaacat agccattttt ttgttgcgca tactccgatg catcaacact tcgcgataca
     1261 tccaccaaca aagccaactc caagctaaca ggtatagcaa acagttgctt ataactaact
     1321 aatagtatta gtagcataaa aaaactggca attctttgct ttctcatcat gtctctccat
     1381 acttaaaata taaaatcaat cccctggtga tcgtgaagta acgcaaaatc taggccaacc
     1441 attgtaattc attgatatag aatggaatct tagaatagaa cgtcattttg gctttaagtt
     1501 tgtaaatatc cttgacaaaa atactaacga ctgtaccaat aacttcattt catttaaatt
     1561 tagacttaat tcttcctgta ttgatatatg cgaattaaac ccgagcaact ccctcagtta
     1621 ttgaaaaaac aacaaaacca gtgttttacg atcatggggg atgaaccttt attatcgatc
     1681 gaagcggcgg ataccattcg ccattccctt tattatcaag gtgtgactga gcgagaaatt
     1741 ttcacagtag atcatcgctt cgaatggagc aaaatcacgc aatggagtga tcaatgttct
     1801 ttgtttagca cctctaaatt gcttgatctc cgaattccga caggcaaagt aggcaatgat
     1861 ggcaacactg cacttgtaac ttttcttgat aatctctcgc ctgatttatg gtttatggca
     1921 acgttaccga cgattgataa aaaaagccaa gcaagtaaat ggtttactgc attagataac
     1981 cgcggtgtga tcgtagcaat cgattcaatc gaacgaaccc aacttccaca atggattcag
     2041 cagcgattcc atgcgcaaaa gcaaaccata gcacccgatg cattacaatt gttagtcgat
     2101 aacgtagaag gaaatttact ggccgcacaa caggaaatcc tcaagcttgg tctcctttat
     2161 cccgaagcaa cgctttctgt tgagcaagtc agaaatgctg tgtttgacgt cgcacgacat
     2221 gatgtattcg atttacctga agccatgctc actggggata ttgtgcgctt tacgcatatg
     2281 ctggaaaatc tacacagtga aggtgctgct ccccccctgg tcttgagtat cctcgtcgac
     2341 ccactccgaa aactattgtt actccaccgc ggtgttagtg aaggaaaatc gctagggtac
     2401 ttattcaagg aaattcgagt ctggggtcaa caacagacat tattatcttc agcgatgaag
     2461 cgctatgccc cccctttatt aatggagggg ctgacgcatg ctgcgctgat cgataaaatg
     2521 atcaaaggga ttaaacaagg tgaaatctgg gatgaattcc tacaatatgg actgcgtttt
     2581 gccgagcgca gccaaacaaa atgatttgaa cggcgtatac tgcaaactta acaagaatca
     2641 atcaatttta gaaaaataat gaaagctaaa atcaaatggc aagaaggtgt tagtttctct
     2701 ggagaaaccg aaagtggaca tacgctgatc atggatggcg cacctgaagc aggtggtcgt
     2761 aatctaggac cacggcctat ggaaatgatt ttactgggtc tgggtggatg tactgcgttc
     2821 gatgtggtaa tgatattgca aaaagggaga cattcagtaa cggattgttt cattgaaatc
     2881 gatgcaaagc gtgcaacgga ggaccctaaa gtgtttaccc aaattcatct ccacttcatc
     2941 gtatctggga aaaatttagc agcacaccat gtcgaaagag ccatcgacct ctctgccgaa
     3001 aaatattgtt ctgcctcgat tatgctgaga agtacagcca atatcacaca tgattttgaa
     3061 atcattgacg tctaacccca aaacttctac cttacagact cagtttttaa ctgttactgc
     3121 ggtatagttt tgatcataat cacgtaaaat tctcatcaac aacctaaata atttatgcca
     3181 tgaataacga taataatgat gatcataaat cggatgaatc tttacacgag aatatgcgct
     3241 attttctttt ctggatgata gagcagcaag agttactcca tcaaaagaat gtcaaaaaag
     3301 cgcagtgttt tatcgcgctt attagaacgc tgcattttgt caaaacccag ccttgttcat
     3361 cgaatactca taaagtttct acgcagttta tagccagaaa attaaatcag cttagctgtc
     3421 attagctacg ataaccagta cggttatgca atttccaatg agaaaaattc tcagatcaag
     3481 catcagccac gctggatgtt aaaaatagat catggaaatg attaccgaac gaattgggaa
     3541 tgaattagca aagttcttat caaagccagt aagacaaagc atccaggtcg caatgatctc
     3601 tcaagaacag ctgctggcta cgctccaacc tgctgacgta cttcttgttg aaggcaatac
     3661 tcggattagt actgcgatta agtatctgac acaatccagt tggtctcatg ctgcgttata
     3721 tattgggaac gcattaccta acgatagccc ttggaattta ccgcaggttc tggttgaagc
     3781 ggatcttaaa gaaggcatac gcgcagtaac cattgcacag tatgcacaaa tgcatacgcg
     3841 aatttgccgt cccatcggtt taaatgatga tgatcggaag catcttattg attatgcgat
     3901 tacgcgtatt ggtcaacaat atgatttaaa gaatattttt gatctagcac gatacttatt
     3961 tccagccccc cccattccaa cacgattccg aagaagatta cttgccctag gcagtggaga
     4021 tccaactcgc gctatctgct cgacactgat cgctcaagca tttatgtcta ttcactaccc
     4081 catcctgccc gaagtccaaa gaacctggtc agacaatcct atgcatgccg attgccacgc
     4141 tgaaatctat catatccgac atcacagctt gtataccccc cgtgattttg atatttcccc
     4201 tttttttgaa attatcaaac cgacgactcg cgatcacttt gattaccacg ctttaacttg
     4261 ggattctgaa acagataaca cttagatcat cagccgccaa agtggccagc actgggggag
     4321 tcaactagtc cactactcct catcagcatt ttcaaaacga acccaagaac caccaacttg
     4381 tgaacgctca gcacccatat cgaccggtaa atctgcgata tcatttaatg ttgcgtcttg
     4441 tgccaaaaga tgtgcaacat ggactacaac taattcactt ctcattgcct cttcatcttc
     4501 gaagtcatgg ttcgcatcgt cacaatacat ttgccagtca ccgcctgcat gtgaaacaaa
     4561 caacaccggt tttttggcct cgaggacaca tcggcacgtg ataacaccta gattatctgg
     4621 aaataacatg gaacctccca attaacatag cgaagatact caaataatgc tcatcttact
     4681 tagccaaaaa ataagatact caaaagagct atcccgcttc tgctcaaaaa taatttaggg
     4741 acaatgcttg cgaatcactt cacttgcgac ggtaataatc attgccaatg aaatagcccc
     4801 aggatcaggt gtaccgatgc tcttttctgc gtgaggacgc gcacgaccaa tgcgcggcag
     4861 caaattgcgg gtttgttgcg ctgctgtatt cgcaatatca gcagcatctt gccacgcagt
     4921 taccactgaa ataccccttg cagaagcttg atcaagtgca gcggaaaaag gttgcaatac
     4981 atccacaaga gtcttgtcac cgggctgcgc tttaccacgc tgcattacct cgcatagtgc
     5041 agcagctaca ctacctgcaa cagttgcagt atcaggtgcg gttgtatcgc ctaatttaac
     5101 tccgaatgta ttaagtatta caccccacag cgccccagac gtaccccctg cgcgtgcagc
     5161 ccaggcctct ccagacaagg ttagtaatgt tccagcgcca gcttgtgcat catgggcttt
     5221 tttcgcggca tcaagcgctg ctgatacacc ccttagcata ccgataccat gatcaccatc
     5281 acccgccaca gcatccagac gcccgagttc ttcttgtccg agacgatcca cgcggccttc
     5341 gtgccgtcct tcgagagcag gtgcacgggc tgaatcacgt attgatcgag caccttcgtc
     5401 tcgcccgtcg cgaagttcgt gagcagcacg tcgaagagct cgtcggtcat gtgcgggctg
     5461 gtggtgatcg cgcgcgagcc gtcctccgac agcttgaagt tgaaggtgtt ctgcgcggtc
     5521 ttgcggggct tctcgacgcc cgcctt
//
LOCUS       DAOQKQ010000167         5518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_7812_length_5518_cov_5.596376, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000167 DAOQKQ010000000
VERSION     DAOQKQ010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5518
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7812_length_5518_cov_5.596376"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="PKZ49_10255"
     CDS             complement(<1..680)
                     /locus_tag="PKZ49_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HND36987.1"
                     /translation="MSKLTESAIETLAIQLFEQLGYSAIHAPDISPDGDHPERTRYDE
                     VLLTGRLTSALNRINPGMTDAVLHAALKEVERIHSPELLNNNETFHRLLTEGVKVSTQ
                     HEGNERGEIVWLVDFDTPDHNEFVVASQFTVIENNQNKRPDLVVFVNGIPLVVIELKN
                     AADENATLQSAFKQISTYKQAIPGLFTYNALLVISDGLEAKAGSLSAGFSRFMTWKIL
                     DDVDGMVGA"
     gene            complement(673..1047)
                     /locus_tag="PKZ49_10260"
     CDS             complement(673..1047)
                     /locus_tag="PKZ49_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HND36988.1"
                     /translation="MILVDTSVWINHLRNNDPYLVRLLTENSVLGHPFVRSELALGNL
                     RQREIILAALDNLPQAPVAFTDEVNYFIEKHALYGLGIGLIDVHLLASTLLSGNTRLW
                     TQDKRLQAAALRLNLAALVHHV"
     gene            complement(1056..1250)
                     /locus_tag="PKZ49_10265"
     CDS             complement(1056..1250)
                     /locus_tag="PKZ49_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HND36989.1"
                     /translation="MRTTIVLDDELLEKAQALTHVQEKSALVKEALKALIERESAKRL
                     ANLGGSEPQLEAIPRRQTSE"
     gene            complement(1331..1867)
                     /locus_tag="PKZ49_10270"
     CDS             complement(1331..1867)
                     /locus_tag="PKZ49_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND36990.1"
                     /translation="MTDNPTSHRRKSIRLQGYDYSQAGLYFITVCTHNRVPLFGEIVD
                     GVMALNTAGQIVEKCWCTIPEHFPQVTVDEFVVMPNHVHGIITIGTIVGANDYLPLPS
                     NETPRSLQHGTSRTIGSMVRGFKIGVTRWFRANTDIHKVWQRNYYEHIIRNEDSYLKI
                     ADYIQTNPQRWEIDTYYV"
     gene            complement(1916..2923)
                     /gene="rhuM"
                     /locus_tag="PKZ49_10275"
     CDS             complement(1916..2923)
                     /gene="rhuM"
                     /locus_tag="PKZ49_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HND36991.1"
                     /translation="MIEPIDNIVIYQPDDGLLGVEVRLRDETAWLSQAQMAALFDKDS
                     DTIGLHLKNIYQSGELIESATTEKSSVVRQEGGRQVRRNIKVYNLDAIISVGYRVNSK
                     KGTQFRIWASNILKRYLVQGYALNEQKLQMQQQKLTDLKQAIALSSRLLRQKDLTVSE
                     SQGILSILEKYSHALTVLDDYDHQRLQVTGIQQTGQSRISYDEAIQQIRLWREQEKLS
                     GLFGNEKDDSFKSSLETIYQTFGGTELYPSVEEKAANLLYFIVKNHSFSDGNKRIAAA
                     LFVWFLARHDYLLNADGEKRIADNALVAFTLLIAESKPEEKDTMVKVKVIINLINGNN
                     P"
     gene            complement(2920..4083)
                     /locus_tag="PKZ49_10280"
     CDS             complement(2920..4083)
                     /locus_tag="PKZ49_10280"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HND36992.1"
                     /translation="MSKWQECRLGEVAEIQTGPFGSQLKNSQYVTGGTPVITVEHIDN
                     FRIIDFDYPSVTYEDKKRLSKYLLNQGDIIFTRVGSVDLSAYVQPHQQDWMFSSRMLR
                     VRSDQRINSRFLSYFFQQNSFREYILSIAVGATMPSINTEILKSVPISYPSLPEQKAI
                     SSVLSSLDDKIDLLHRQNKTLEAMAETLFRQWFVEEDSGFREGCLADVAINVKEAIKA
                     SDLKSESKYIGLEHIDQRNIALKHYGFGSEVSSNKYAFQRHDILFGKLRPYFHKVCFA
                     PFSGICSTDILVIRPIKSELFAFCLFAFFQDEVVEYANLSSGGTRMPRTDWTTLKNYP
                     IAIPDSYSIERFNNAVSSSLKKISSNLEQIRTLETLRDTLLPKLMSGEVRVTV"
     gene            complement(4080..>5518)
                     /locus_tag="PKZ49_10285"
     CDS             complement(4080..>5518)
                     /locus_tag="PKZ49_10285"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HND36993.1"
                     /translation="GADPEDKDEYQAENVFFVPAEARWPYLVAHAKQPDIGTHVDAAM
                     DAIEQENPSLKGVLPKVYARQNLDPTSLGELIDLIGNIALGDAKARSQDVLGHVFEYF
                     LGEFALAEGKQGGQFYTPRSIVELLVNMLEPYRGRVFDPCCGSGGMFVQSEKFVEEHQ
                     GRVDDISIYGQESNQTTWRLAKMNLAIRGIDSSQVKWNNEGSFLNDAHKDLKADFIIA
                     NPPFNVSDWSGEQLRGDARWQYGTPPPGNANFAWLQHFVYHLSPTGIAGVVLAKGALT
                     SKTSGEGDIRKNLIVEGNLIDCIVNLPAKLFLNTQIPAALWFLNRNRAGTTVGVNDVR
                     ANNVGANNHLPLQHDHPHKNEILFIDARNLGHLINRRTRELSHDDIHKIASTYHAWRN
                     AAVVPAQAGIQPYEDIKGFCASVPISRVAELDYVLTPGRYVGLPDEEDDFNFPERFAA
                     LRAEFEAQLREEAVLNQAIVESLARVKV"
BASE COUNT         1359 a         1341 c         1298 g         1520 t
ORIGIN      
        1 gcccctacca taccgtcaac atcatcgaga atcttccacg tcatgaaacg gctgaatccc
       61 gccgataacg aaccggcttt ggcttccaat ccatccgaaa tcaccaacaa ggcattgtaa
      121 gtaaacaaac cagggatggc ttgtttgtag gtgctaattt gcttgaaagc ggattgtagc
      181 gtggcgtttt catcggcggc atttttaagc tcgatcacca ccagcgggat accattcaca
      241 aataccacca gatccgggcg cttgttctgg ttattttcta tcaccgtgaa ctggctagcc
      301 acgacaaatt cgttatggtc gggggtatca aaatcaacta accagacgat ctcgccacgt
      361 tcgttgcctt cgtgttgtgt gctaactttg acgccttcgg tcagcaggcg atgaaaggtt
      421 tcattgttgt tgagtaattc aggtgaatga atacgctcaa cttccttcaa cgcagcgtgt
      481 aataccgcgt ccgtcatgcc aggattgatg cgattaagcg cacttgttaa gcgcccggta
      541 agcagcacct catcataacg ggtgcgttcc ggatgatccc catcagggga aatatcgggc
      601 gcgtggatag cgctgtaacc aagttgctcg aacagttgga tcgctaaggt ttctatcgct
      661 gattcggtga gtttagacat ggtgaaccag tgccgccaaa ttaagacgta atgcagctgc
      721 ctgaagccgc ttatcctgtg tccaaagccg agtattgccg gataacagcg ttgaagctaa
      781 cagatgcacg tcgatcaatc cgatacccaa accatataaa gcatgttttt cgatgaaata
      841 attcacttca tcggtaaaag cgacaggggc ttggggcagg ttatccagcg cagccagaat
      901 gatttcacgt tggcgcaagt tgcccagcgc cagctcgcta cgaacaaagg gatgacctaa
      961 tacgctgttc tcagtcagca ggcggactaa atacggatcg ttattgcgca gatggttaat
     1021 ccagaccgaa gtatcgacca gaatcattgc ggcagctatt cgctggtttg tcggcgggga
     1081 atggcttcca gttgcggttc agagccaccc aaattagcca gccttttggc actttcccgc
     1141 tcgatcaggg ctttcaaggc ttctttaacc aaggctgatt tttcctgaac atgggtcagg
     1201 gcttgcgctt tttccagtaa ttcatcatcc aatacaatgg tagttctcat gccttatctc
     1261 cagatatgtc atcgaatgat gcgctttatt gtgcacgaag acaagtttaa gggcaaatca
     1321 tcatttactc ttaaacataa taagtatcga tttcccaacg ctgcggatta gtttgaatat
     1381 agtctgcgat tttcaggtaa ctatcctcat tgcgaatgat atgttcgtaa taattgcgct
     1441 gccaaacttt atgaatatcc gtattggcgc gaaaccaccg ggtaacgcca attttgaatc
     1501 cccgcaccat cgatccaatg gtgcgggatg ttccatgttg caatgaacgg ggtgtttcgt
     1561 tggatggtag gggcaaataa tcatttgccc caacgattgt accgatggta ataatcccat
     1621 gcacatgatt gggcatcacc acgaattcat ccactgtaac ctgcggaaaa tgctcgggta
     1681 tcgtacacca gcatttttct acgatctgac ccgctgtatt caacgccata accccatcaa
     1741 caatttcccc aaacaatggc acccggttgt gtgtgcaaac agtaatgaaa tacaaaccag
     1801 cctgtgaata atcatacccc tgcaatcgaa tcgatttgcg gcgatggctg gttgggttgt
     1861 cggtcatggt gtccgcgtta ttgttgggat agtaggggca aatgattatt tgcccctacg
     1921 gattattgcc attgattaaa ttgatgatga ctttcacttt caccatcgtg tctttttcct
     1981 cgggtttgct ctcggcaatc aacagggtaa acgcaaccag ggcattatca gcgatgcgct
     2041 tttctccatc cgcgttaagc agataatcat gacgcgccag aaaccagaca aacagcgcgg
     2101 cggcaatgcg tttgttgcca tcggaaaaag aatgattctt gacgatgaaa tacaacagat
     2161 tagccgcttt ttcttcaaca cttggataga gttctgtgcc gccaaacgtt tggtaaatgg
     2221 tttccaacga gcttttgaag gaatcgtctt tttcgttgcc gaacaatcca ctcagtttct
     2281 cctgttcacg ccacagccgg atttgctgaa ttgcttcatc ataggaaatt ctgctctgtc
     2341 cggtttgctg gattcccgtt acttgtaagc gctggtgatc gtaatcgtcc agcactgtca
     2401 atgcgtggct gtatttttcc aggatcgata aaataccttg cgattcggaa acagtcaggt
     2461 ctttttgtcg caacagacgt gaagataaag caatcgcttg ttttaaatcc gtgagttttt
     2521 gctgttgcat ctgaagtttc tgttcattca gcgcataacc ttgcaccagg taacgtttga
     2581 gaatattgct ggcccagata cggaactgcg tgcctttttt ggagttgacg cgataaccga
     2641 cggaaatgat ggcatcgagg ttgtaaacct tgatgtttct tctgacctgc cgcccacctt
     2701 cttgtcgaac taccgaggat ttttcggtag ttgccgactc aatcaactca ccggattggt
     2761 aaatattctt caggtgcaac ccgatggtat cgctatcctt atcaaacaag gccgccatct
     2821 gtgcctgcga caaccaagcc gtttcgtcgc gcaagcgcac ttccaccccc aacaacccat
     2881 cgtccggctg gtaaatcacg atattgtcta tcggttcgat catacggtta cccgcacctc
     2941 gccgctcatc aatttgggga gcaaggtgtc gcgcagggtt tcgagggtgc ggatttgctc
     3001 taaattagaa gaaatctttt tcaatgaaga agaaacagca ttattgaagc gttcaataga
     3061 ataagaatct gggatagcta ttggataatt ctttaatgtt gtccaatcag ttctcggcat
     3121 tcgcgtcccc ccagaactca aattggcata ttcaactacc tcatcctgga aaaatgcaaa
     3181 aagacaaaaa gcaaacaatt ctgattttat tggcctaatt accaaaatat ccgttgagca
     3241 aatacctgaa aatggtgcga aacaaacttt atggaaatat gggcgtaatt tgccaaataa
     3301 gatatcgtgc ctttgaaatg catacttatt actgctgact tcagaaccga aaccataatg
     3361 tttcagtgca atatttcttt gatcgatatg ttccaaacca atatattttg attccgattt
     3421 taaatctgat gcttttatag cttctttaac gttaattgca acatcagcta aacagccttc
     3481 cctgaaaccc gaatcttcct ctacaaacca ctgcctaaac agcgtttcag ccatggcttc
     3541 gagggttttg ttctggcggt gcagcaggtc gattttgtcg tccaagctgc tgaggacgga
     3601 gctgatggcc ttttgttctg ggagtgaagg gtatgaaata ggaacacttt tcagaatttc
     3661 tgtattgatt gatggcattg tagctccaac agcaatactt agaatgtatt ctctaaatga
     3721 gttttgttga aagaagtagc tcaagaatct tgaattaatt ctttgatctg acctaacacg
     3781 aagcattctg gatgaaaaca tccagtcttg ctgatgaggt tggacgtaag cgctcaaatc
     3841 aacagaaccg actctagtaa aaataatgtc accttgattc agtagatatt ttgataatct
     3901 ttttttatct tcataggtaa cactgggata gtcgaagtct ataatcctaa aattatctat
     3961 gtgttcaaca gttattactg gtgttccgcc agttacatat tggctatttt tgagttgact
     4021 accaaaaggt cctgtctgaa tttctgcaac ttctcctaat ctacattcct gccacttact
     4081 cacactttca ccctcgccaa gctctcaaca atcgcctgat tcagcaccgc ttcttcccgc
     4141 aattgcgctt caaattctgc cctcagcgca gcaaaacgtt cgggaaaatt aaaatcgtct
     4201 tcttcgtctg gcaggccgac gtagcgtccc ggtgtcagca cgtaatccag ttcggcgacg
     4261 cgcgaaatcg gcactgaggc acagaagcct ttgatgtctt catacggctg gattcccgcc
     4321 tgcgcgggaa cgacggcggc attgcgccag gcgtggtagg tgctggcaat tttgtggatg
     4381 tcgtcgtgcg acaattcgcg cgtgcggcgg ttgatcaggt ggccgaggtt acgggcgtcg
     4441 atgaacagaa tttcattctt gtgcggatga tcgtgttgta ggggcaaatg attatttgcc
     4501 ccaacgttat ttgcccgtac atcatttacc ccgacggttg ttcccgcgcg attccgattc
     4561 aaaaaccaca gtgccgcggg aatctgcgtg ttcagaaaca atttggcggg caaattgacg
     4621 atgcagtcga tcaggttgcc ttccacaatg aggtttttgc gaatgtcgcc ttcaccggag
     4681 gttttcgagg tgagcgcgcc tttggccaat accaccccgg caatacccgt aggcgacagg
     4741 tggtagacga aatgttgcaa ccaggcgaag ttggcgttgc cgggcggcgg cgttccgtat
     4801 tgccagcgcg cgtcgccgcg cagttgttcg ccgctccaat cgctgacgtt gaacggcgga
     4861 ttggcgatga tgaaatcggc tttcaggtct ttgtgggcgt cgttcagaaa cgaaccttcg
     4921 ttgttccatt tgacttgcga gctgtcgatg ccacggatgg caaggttcat tttcgccaac
     4981 cgccaggtgg tctggttgct ttcctgcccg tagatagaaa tatcgtcgac gcggccttgg
     5041 tgttcttcga caaacttttc cgattgcacg aacattcccc ccgacccaca gcatggatca
     5101 aatacccgtc ctctatacgg ctccagcata ttcaccagca attcgacgat gctgcgcggg
     5161 gtgtagaact ggccaccttg cttgccttcg gccagtgcga attcaccgag gaaatactcg
     5221 aacacatggc cgagtacgtc ttggctgcgt gctttggcat cgccgagggc gatattgccg
     5281 atcaggtcga tcaattcgcc caggctggta ggatcaaggt tttgccgcgc gtaaactttt
     5341 ggcagcacgc ctttaagcga gggattttcc tgttcgatcg cgtccatcgc ggcgtcgaca
     5401 tgggtaccga tgtcgggttg cttggcgtgc gccactagat acggccagcg cgcttcggca
     5461 ggaacgaaga agacattttc cgcctggtat tcgtccttgt cttccggatc ggcgccag
//
LOCUS       DAOQKQ010000168         5507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_7842_length_5507_cov_5.146735, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000168 DAOQKQ010000000
VERSION     DAOQKQ010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5507
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7842_length_5507_cov_5.146735"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1750)
                     /locus_tag="PKZ49_10290"
     CDS             complement(<1..1750)
                     /locus_tag="PKZ49_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HND36994.1"
                     /translation="MEQNRGKGEIRKLVALWQFLKPYQLRFFFALIAMVIAAGSVLVL
                     GTALKHVIDEGYVLGDMAALNRTLSIVAIVALVQGVATFIRYYLFTWVCERFVADIRK
                     TVFGHILNLSPTFFEKTRTGEVISRLTNDTQRIETVVIGVFSFALRNFVMMIGGIVML
                     AITSLKLTLIILAAVPLVVTPILLLGRRVRKTSEEMQNRVADSSSYIDETIHEIRVVQ
                     AYTHEAVDRDRFADQIERVFKAGVEAGRYRAAMIASVIVLAFSSIAFVLWIGGRAVIT
                     GEISPGDLSAFLFYAILVANGVMAISETWSELQRAAGASERLMDLLSTSPEIKAPQHP
                     KVLPAQTPLTGEQDNQPYKAPIGCVRFEQVTFYYPSRTETAALQNFSLEIQPGEIVAF
                     TGPSGAGKTTVFQLLLRFYDPQQGRLLVEGVDIRELEPTGLRQHFSIVTQEPIVFADS
                     VLENVRYGRPHATREAVIDACQAAYCMQFIEKLPHGLDTQLGERGTKLSGGQRQRIAL
                     ARAFLADRPILLLDEATSSLDAESEQMVQQAMDKLIHTRTTLVIAHRFSTIQSADRII
                     VMDHGTIQAMGTHRELI"
     gene            2221..2697
                     /locus_tag="PKZ49_10295"
     CDS             2221..2697
                     /locus_tag="PKZ49_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HND36995.1"
                     /translation="MALTLTSPAFAHNGLIPAQFTCDSQDLSPALAWSGLPEGTKSLV
                     LIVDDPDAPDPAAPKMTWVHWVLYNIPPQVAGLAEAIAPAQLPAGTLEGINDWKRTGY
                     GGPCPPIGVHRYFHKLYALDTVLPDLKQPTKTQLEKSMQGHILGHVELVGRYQRNQ"
     gene            complement(2723..3610)
                     /gene="argB"
                     /locus_tag="PKZ49_10300"
     CDS             complement(2723..3610)
                     /gene="argB"
                     /locus_tag="PKZ49_10300"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HND36996.1"
                     /translation="MSLSPPTAKEKAKTLAEALPYIQRFHGKTIVIKYGGNAMTEEDL
                     KRGFARDVVLLKLVGLNPVVVHGGGPQIDQMLKRVGKEGVFVQGMRVTDAETMDVVEM
                     VLGGLVNKDIVNLINRHGGRAVGLTGKDGSFIRAKKMLIEDKEKAGGWINIGQVGEIE
                     SIDPALIALLDTRDFIPVIAPIGVGEDGQSYNINADLVAGKLAEVLKAEKLILLTNTP
                     GVLDKNGNLLTGLTTKRVDELFADGTISGGMLPKISSALDAVRNGVRSCHIIDGRVPH
                     ALLLEILTDEGVGTLISSV"
     gene            complement(3680..4198)
                     /locus_tag="PKZ49_10305"
     CDS             complement(3680..4198)
                     /locus_tag="PKZ49_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HND36997.1"
                     /translation="MFNHSGMREYQLALTERINSATNAPSPAIPMLGVSIGEERWLVP
                     MNSISEVLPVPKITPVPLTHRWFLGVINVRGYLYGISDIAAYLGHALTPLTPKSRIIL
                     FAPRLGANSGLLVSNLLGIRNLNEFKQIEDHDVAHATIKARQFVDKQDRPWLELNLRA
                     LIHEQSFIRIDR"
     gene            complement(4203..4568)
                     /locus_tag="PKZ49_10310"
     CDS             complement(4203..4568)
                     /locus_tag="PKZ49_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HND36998.1"
                     /translation="MSIRKILVIDDSPTDRHVLRNILIENGYQVGIAESGEEGVSKIK
                     ESMPDLVLMDVVMPGMNGFQATRVLSRDETTQHIPIILCTSKGQETDKIWGLRQGARD
                     YITKPIIAEELLQKISQLN"
     gene            4809..5480
                     /locus_tag="PKZ49_10315"
     CDS             4809..5480
                     /locus_tag="PKZ49_10315"
                     /inference="COORDINATES: protein motif:HMM:NF014037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HND36999.1"
                     /translation="MDFTAYFRFYAELNDFLPPIQRQQVITYRFNGHPGIKDPIEAQG
                     IPHPEVELILVNDQPVDFSYQLRDTDRVAVYPKFIQLNLSGLPRLRSELPNPVRFIVD
                     VNLGKLAKLLRLLGFDSLYCNHFQDSVIVATAVAEQRIILTRDRRLLFAKKIEHGYWI
                     RAVEAKKQTHEVLNHFELHTAIQPFSRCLTCNGLLAVMPKASIIEQLEPKTKQYYDTF
                     YQCTL"
BASE COUNT         1500 a         1381 c         1148 g         1478 t
ORIGIN      
        1 cgattaattc gcggtgggta cccatcgctt gaatagtgcc atgatccatg acgataatgc
       61 gatccgccga ttgtatcgtg gaaaaacgat gcgcaatcac caatgtagtc cgagtatgaa
      121 tcagcttatc catcgcttgt tgaaccattt gttcactttc tgcatcgagg cttgaggtcg
      181 cttcatcgag cagcaatatc ggccgatcgg cgagaaacgc ccgtgccaaa gcaatccgtt
      241 gccgctgccc accggagagt ttagtgccac gctcaccgag ttgagtatca aggccgtgag
      301 gtaatttctc aataaactgc atgcaataag ctgcctgaca cgcatcgatt actgcttctc
      361 gcgttgcgtg tggtcgacca taacgcacat tctccagtac gctatccgca aaaacgatag
      421 gttcttgcgt aacaatcgaa aaatgttgtc ttaatcccgt tggttcaagt tcccgaatat
      481 cgaccccttc aaccaacaag cgaccttgtt gcggatcata aaaacgcaac agtaattgaa
      541 atactgtggt tttacctgcg cccgatggtc ctgtaaatgc cacgatctct cccggttgaa
      601 tttctagcga gaaattttgc aatgcggccg tctcggtccg actgggataa taaaaagtaa
      661 cttgttcaaa acgcacacat ccaatcggcg ctttgtaagg ttgattatcc tgttcaccgg
      721 ttaaaggggt ttgcgctggt aacactttag gatgttgagg cgctttaatt tcgggcgagg
      781 tggaaagcaa atccattaat cgttcagatg cccctgccgc gcgttgcaat tcactccatg
      841 tttcagaaat tgccatcacg ccattcgcaa ccagtattgc atagaataga aacgccgata
      901 gatccccggg cgaaatctca ccggtaatga ccgcacgccc accgatccaa agcacaaacg
      961 cgatggatga gaaagccagt acaattacac tcgcgatcat cgcggcacga tagcgcccag
     1021 cttcgacacc cgctttaaat acgcgctcaa tttgatcagc aaagcgatct ctatctactg
     1081 cttcatgggt ataagcttgc acgactcgaa tctcatgaat ggtctcatcg atataactcg
     1141 acgaatccgc gactcgattt tgcatttcct ccgacgtttt acgaacgcga cgccccagca
     1201 gtaagatagg ggttaccact aaaggaacgg ctgccagtat gatgagcgtc aatttcaaac
     1261 tggtgatcgc aagcatcacg ataccgccga tcatcatcac aaaattgcgc aacgcaaagg
     1321 aaaaaacacc aatcacaacg gtctcaatgc gttgagtatc gttggtcaat cgtgaaataa
     1381 cttccccggt gcgggttttc tcaaaaaatg taggcgatag gttgagtatg tgaccgaaca
     1441 ccgttttgcg aatatcggct acaaaccgtt cacaaaccca ggtaaacaga taatagcgaa
     1501 tgaaggttgc aaccccttgc acaagagcaa ctatcgctac aatcgataat gttcggttga
     1561 gcgctgccat atctccgagt acatacccct catcgatgac gtgtttcagc gcggttccta
     1621 acacaagcac tgagccagca gcaatcacca tagcgatcag cgcaaaaaag aaacgcaatt
     1681 ggtagggttt caggaactgc cacaaagcaa cgagcttacg gatttccccc tttcctctgt
     1741 tttgttccat tgaatagtaa cgcttcacca tcgtcttaaa aaatccgagt gattgaaaaa
     1801 gttattaata ggcatgttct caattgtcaa aagcttatcg attattcatc cctcgatgaa
     1861 cgctgtgatg ttatatgggt ttatcgcata gcctatctta tcaaattggc tgcgattgtt
     1921 gtcgactaca gcctggttct acatcacact ctgcagatca tcagcaatta attcaagcgc
     1981 gtcgagataa cgatttttta gtaatacgat cggttataat ctttgctaac aacttgcttg
     2041 ctaccgtcat cagaaaggag tttaaccatg aaaggacgtt cgactgctta ttcccggatc
     2101 aatggtaaga tcagtttatc gaaaaatttc agcattacgc tggccttgct ctggtttgcg
     2161 atactagtcg gaacatccca ggcagacacg acactttcac aaaacaatac ggagaatacc
     2221 atggcgctta ctctgacctc acctgccttt gcacataatg gcctgattcc agcccagttt
     2281 acctgtgata gtcaagacct ttcacccgcc ttggcttggt cagggttacc tgaaggtaca
     2341 aaaagtctag tgctcatcgt ggatgacccc gacgcgccag atcctgcagc accaaaaatg
     2401 acctgggtac attgggtact ctataacatt ccgccacagg tcgctggatt agctgaagcc
     2461 attgcgccag ctcaattacc tgcaggaacc ttggaaggaa tcaatgactg gaaacgaacg
     2521 ggttatggcg gtccttgccc accaatcggt gttcaccgct attttcacaa actttatgcc
     2581 ttggatacgg ttttacctga tttaaagcag cccaccaaaa cgcagctaga gaaatccatg
     2641 caaggccaca ttctaggcca cgttgaatta gttgggcgct atcagcgcaa tcaataaatc
     2701 cagcaatagc ctagttattg cattaaaccg aagaaatcag cgtccccacg ccctcatcgg
     2761 tcaagatttc caatagcagt gcgtgcggaa cgcggccatc gatgatatgg caagatctaa
     2821 cgccatttct aacggcatcc aacgcggagc tgattttggg tagcatgccg cccgaaatgg
     2881 ttccatctgc aaaaagctca tcgacccgct ttgtcgtcag accggtgagt aaattgccat
     2941 ttttatccaa tacccctggg gtattcgtta acaaaatgag tttctcagct ttcagcactt
     3001 cagccaattt acccgcaacc aaatcagcat tgatgttgta ggattgacca tcctcgccaa
     3061 caccaattgg agcaatcacg gggataaaat ctcgcgtatc gagcaaggca atcaaagctg
     3121 ggtcaatcga ttcgatttct ccgacttgac cgatattaat ccaccctcct gctttttcct
     3181 tatcctcaat cagcattttc ttggcgcgga taaatgagcc atcttttccc gttagaccga
     3241 ctgctcgacc gccatgccga ttaatcaaat tcacgatatc tttattgacc aagccaccga
     3301 gcaccatttc taccacgtcc atggtttctg catcggtcac gcgcatccct tggacaaata
     3361 caccttcttt cccgactcgt ttgagcattt gatcgatttg cggcccgcct ccgtgtacca
     3421 caaccggatt taatcctacc aatttgagca acaccacatc acgtgcaaaa ccccgcttta
     3481 ggtcttcttc agtcatggca ttcccgccat atttgattac gatggttttt ccatggaaac
     3541 gctggatata aggaagcgct tctgcaagcg ttttagcttt ttcttttgca gttgggggtg
     3601 ataaagacat ggtgttaatc cggagcagtc gtaaaaaaat gacttattct actgcaagct
     3661 gggttaagct aacagcgcgt tatctgtcga tacggataaa cgattgttcg tgaatcaatg
     3721 cccgcaaatt gagttctagc cagggtcgat cttgcttatc gacaaattgc ctggctttaa
     3781 tcgtggcatg agccacatcg tgatcttcaa tttgcttaaa ttcatttaaa ttacgaatcc
     3841 cgagcaaatt actgaccagt aaaccgctat tggcacccag gcgaggcgca aacaagataa
     3901 ttctcgattt gggggttaat ggggttagcg catgacccaa ataagctgcg atatcggaaa
     3961 ttccataaag gtaaccacgt acattgataa cccccagaaa ccagcgatga gtgagtggaa
     4021 cgggcgtaat tttcggaaca ggcagtacct cgctaatgct attcatgggt accagccaac
     4081 gctcttctcc aatactaacg cctagcatcg gaatcgctgg ggatggggca ttggtagcgc
     4141 tattgatccg ttcggtcagc gctaattgat attctcgcat tccagaatga ttgaacacgc
     4201 ggctagttca attgtgatat tttttgcagc aactcttctg caatgatcgg cttcgtaata
     4261 tagtcgcgcg caccctgacg aagcccccag atcttatcgg tttcttgtcc ttttgaggta
     4321 caaaggataa tcggtatgtg ctgcgtcgtc tcatccctgg ataacactcg cgtagcttgg
     4381 aagccattca ttccaggcat caccacatcc atgagcacta aatctggcat cgattcctta
     4441 attttactga cgccttcttc cccactctcg gcaataccca cttggtaacc attttcaatc
     4501 aagatattgc gcaatacatg acgatccgtc ggagaatcat caatcactaa aatttttcga
     4561 attgacatga acgttataaa ttttcgtaac ggtaaataaa ataattcaaa tcgacccttt
     4621 gctgttactc attcggtggg tcgcatcgtg ctacagcgct aagcccactc ttgcatcaat
     4681 gatattagat ataactccat tttgcggtaa gatcaagaaa aaatgcctac tgacaggcat
     4741 aatcatcatt ttttgttgta aatctgctta ctacgctgtg aattcagggt aatcaaacgt
     4801 tgatctatat ggactttact gcttatttcc gcttctacgc tgaattaaac gattttcttc
     4861 ccccgataca gcgtcagcaa gtgataacct atcgtttcaa tggccatccc ggtatcaaag
     4921 atcctataga agcacaaggc atccctcatc ccgaggtcga gcttattctg gttaatgacc
     4981 agccagtcga ttttagctat caactgcgag atactgatcg cgttgcggtt tatcctaaat
     5041 ttatccagct taatctttcg gggttgccac gattacgttc tgaattacca aaccctgtgc
     5101 ggtttatcgt ggatgttaat ctgggtaagt tggctaaatt attgcggtta ctcggttttg
     5161 acagtttata ctgtaatcat ttccaagatt ccgtcatcgt tgcaacggct gttgctgagc
     5221 aacggatcat cctgacacgc gatcgtcgct tattatttgc caaaaaaatt gagcatggct
     5281 attggattcg cgcagtcgaa gccaaaaaac aaacgcatga agttttgaat catttcgaac
     5341 tccatactgc aatacaaccc ttttcccgtt gtttaacttg caatggatta ctcgctgtca
     5401 tgcccaaagc gagcatcatt gaacagctcg aacccaaaac gaaacaatac tacgacacgt
     5461 tttatcagtg cacactttga cagaaaattt actgggaagg ctcgcat
//
LOCUS       DAOQKQ010000169         5443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_7994_length_5443_cov_5.528211, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000169 DAOQKQ010000000
VERSION     DAOQKQ010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5443
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7994_length_5443_cov_5.528211"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="PKZ49_10320"
     CDS             <1..512
                     /locus_tag="PKZ49_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37000.1"
                     /translation="GIMHNAEAGELIIRVGFPYMASVTWQAGLFHPSRGGLWLTTSYG
                     KGQWGNNPIKGVQSANVTALSAATYFTLLAQGRLVDDVSSKEMQTWLRGGCMTKHFPQ
                     GLGLVASKCGVWSDYWHDCALIARGRIRYVVVVLSRTKPSQFPKYTQLIRELDNLIVR
                     NNQIPRVAC"
     gene            571..1998
                     /locus_tag="PKZ49_10325"
     CDS             571..1998
                     /locus_tag="PKZ49_10325"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF006053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HND37001.1"
                     /translation="MKPVKQLASPVDLYSPVIDYQQISRARFVEELKAFIHFPSVSAQ
                     PEHDQDTRRCAAWLANHLRKIGMEKVMIVTTQGHPVIYAEKCHIPGKPTVLIYGHYDV
                     QPADPLAEWQFPPFEPVIRGDHLYGRGASDDKGQLFTHVKALEVLLRSGQGLPVNIKC
                     LFEGEEEMGSPHLPAFVVVNQQMLAADCAVVSDTQIPSARQPAITYALRGSINFELEI
                     IGQNRELHSGVLGGAIRNPLQVLCEMIAQLHDRTGRINIPNFYDRVRKWDAQEWAYMK
                     KIEPSDQSILRDAGVGVGWGERGYSIYERTTIRPAITVTSIHGGYQGEGVKAAIPTRA
                     LAKLNFRLVPDQNLREIDQLFRGYLKQVTPPGIRLQIRTSAPSRPVLMDRKHPVVMAA
                     VHAYRHGFGTAPVFLRNGGTIPVVNLIQDILHIPVVLMGFGLPDDRIHGSNEKFYLPN
                     FFNGIKTSIRFLAEVGKWQRTSRVQ"
     gene            2031..2783
                     /locus_tag="PKZ49_10330"
     CDS             2031..2783
                     /locus_tag="PKZ49_10330"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HND37002.1"
                     /translation="MMIIDCHCHAGSGDGFTGPWDTRAPLRDYLRRATVAGIQRSVLF
                     AAFHSDYAAANKEVAKIVASQPDRFYGFAFVHAARDRRRVHELIETAVRQYGFVGIKV
                     HRHDAPITREICEVARAFALPVLYDVVGEVSVCELLAQEYPDVNFIIPHLGSFADDWR
                     AQSAIIDHLVRHPNIYTDTAGVRRFDLLEQAVKRAGAGKILFGSDGPWLHPGIELAKI
                     KALRLSPQQEKLIMGVNLQNLIFPNQKNQAIA"
     gene            complement(2853..4235)
                     /locus_tag="PKZ49_10335"
     CDS             complement(2853..4235)
                     /locus_tag="PKZ49_10335"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HND37003.1"
                     /translation="MDTSQSISIWVFGIGEYGEETFRRFLHLLTQQHIAIQDYCTPCP
                     YSGIIYSNKVTPELLNKLYELSNHGQVQVLACCQEAISGEAIWQLMEAGASDVLVCTK
                     DNLIIHEITARLRRWESISSLLNSPIVQNALVGKSQTLQTILRQVIEVAHFTNVSVLV
                     LGESGTGKELLANLIHTLDNRSEKGELIILDCTTIVPELSGSEFFGHERGAYTGATSS
                     RDGVFALANGGTLFLDEVGELSLTMQTQLLRVIQEQTYKRVGGNAWQRTAFRLICATN
                     RDLATQVQRGKFRADLYYRIASYICHLPPLRERSEDILPLANYFLKKNCPNQDIPIID
                     KPVEEYLLRRQYPGNVRDLKQVIARMLCRYAGKGPITVGCIPPDERPNLSEEWKEWRD
                     MNFERSIRIALSQGMGLKTISKNTEETAIRIAVDEANGNLQRAAQRLGVTDRALQLRR
                     AAQRQENSEP"
BASE COUNT         1424 a         1168 c         1306 g         1545 t
ORIGIN      
        1 agggcattat gcacaatgca gaggctggag agcttattat ccgagttggg tttccgtata
       61 tggcttcggt gacatggcaa gcggggcttt ttcatcccag tcgcggaggt ttatggctta
      121 ccacttctta tggaaaaggt cagtggggta ataatccaat aaaaggggta cagagcgcga
      181 atgtgaccgc gttgtcagcg gctacttact ttacgttgct cgcgcaaggc aggcttgtgg
      241 atgatgtatc gtcaaaagaa atgcaaacct ggttacgagg aggttgtatg accaaacatt
      301 tcccacaagg cctgggattg gtcgcatcca aatgcggtgt gtggtcggat tattggcacg
      361 attgtgctct aatcgcgcgc ggtaggattc gctatgttgt tgtcgttctg agcagaacaa
      421 aacccagcca atttccgaaa tatacccagc ttattcgcga gttggacaac ttgattgtcc
      481 ggaataacca gatccctcgg gtggcctgtt aggtttagaa tggcttgaga atcgatcggg
      541 accaggttta tctaatcaag cgagaaattt gtgaaacctg taaagcagct agcttcgcca
      601 gttgatcttt attctccagt tattgattat cagcaaatat ctcgtgcccg ttttgtagaa
      661 gaactaaaag cattcattca tttcccgagc gtgagcgcac aacctgaaca cgatcaggat
      721 actcggcgtt gtgcagcttg gttagccaat catctgcgta agattgggat ggaaaaagtg
      781 atgatcgtga ccacgcaagg tcaccccgtg atttacgctg agaagtgcca tatcccggga
      841 aaacctacgg tgctcatcta tggccactat gatgtgcaac cagccgatcc gcttgctgaa
      901 tggcaatttc cgccttttga gccggtgatt cgtggagatc atctttatgg aagaggcgct
      961 tcggatgaca aaggccagct ctttacccat gtcaaggcat tggaagtatt gcttcgcagc
     1021 ggtcagggat tacccgttaa tatcaagtgc ttgtttgagg gtgaagagga gatgggtagc
     1081 ccccatttac ctgctttcgt ggtggtcaat cagcaaatgc ttgctgctga ttgtgctgtg
     1141 gtgtcggata cccagattcc atctgctagg caacctgcca ttacttatgc cttaagggga
     1201 tcaatcaatt ttgagctgga gatcattggg caaaatcggg aattacactc aggcgttttg
     1261 ggcggtgcga ttcgtaatcc cttacaagta ctgtgtgaga tgattgcgca attacatgat
     1321 cgcaccggac gcatcaacat tcccaatttt tatgatcgcg tacgaaaatg ggatgcacag
     1381 gagtgggctt acatgaaaaa aatagagcca tccgatcaaa gcattctccg cgatgctgga
     1441 gtgggagtcg gttggggaga gcgtggttat tcaatatatg agcgcaccac gatccgacct
     1501 gcgatcacgg taaccagtat tcatggtggt taccagggag agggtgttaa ggcagcgatt
     1561 ccaacgcgtg cgctggcaaa actgaacttc cggttggttc cagatcagaa tttacgggaa
     1621 attgatcagc tattccgggg gtacctcaag caagttactc caccagggat acggttacag
     1681 attcgcactt ctgcgccatc ccgacctgtg ctgatggatc gtaaacatcc tgtagtaatg
     1741 gctgcggtgc atgcttatcg acacggattt ggaacagcac cagtatttct gcgcaatggt
     1801 ggaacgattc cagtggtcaa tttaattcag gatattctgc atatccctgt ggtgctgatg
     1861 ggcttcggtc taccagacga tcgtattcat ggatcgaatg agaaattcta tttgccgaat
     1921 tttttcaacg gcataaagac gagtatccga tttctggctg aagttggcaa atggcagcgc
     1981 accagtaggg tgcagtagga aaatcaaaag aatagctgat tagggaggta atgatgatta
     2041 ttgattgcca ctgccatgcg ggaagcggtg atgggtttac cggcccttgg gatacgcgcg
     2101 caccattaag agattatctg cggcgtgcca ccgtggcagg aattcagcgc agtgttttat
     2161 ttgccgcatt tcattcggat tacgctgctg ccaataaaga agtagcaaaa attgtagcca
     2221 gccagccgga caggttttat ggatttgcat ttgtgcatgc ggccagagac cgcaggcgag
     2281 tacatgaatt gattgaaacc gctgtcagac aatatggatt tgtaggaatc aaagtgcatc
     2341 gccatgatgc acccattacc cgggagatct gtgaggtggc aagagctttc gctttgcctg
     2401 tgctttacga tgtggtggga gaagtatcgg tttgtgagtt attagcgcag gaatatccgg
     2461 acgttaactt tatcattcct catcttggca gttttgccga tgactggcgt gcgcaatcgg
     2521 ctatcattga tcatttggtg cgtcatccta atatctatac cgatacggct ggagtgcggc
     2581 gttttgattt actggaacaa gcggtgaaac gagctggagc cggcaagatt ttgtttggtt
     2641 cggatgggcc atggctacat cctggcattg aattggcaaa gattaaggca ctacgccttt
     2701 cgccacagca agaaaaattg attatgggag tcaatttaca aaacttaatt ttccctaacc
     2761 aaaagaatca ggcaattgct tgatccaaac agtacaacac agcgtaattc gtttttttgc
     2821 aatcgagtca accggtgatg atagcgtgat catcaaggct cgctattttc ctgacgttgt
     2881 gctgcccggc gcaactgtaa cgcacgatca gtcacaccga gacgttgtgc ggcacgttgc
     2941 aaattgccat ttgcttcatc taccgcaata cgaattgccg tttcttccgt atttttgctg
     3001 atcgttttaa gtcccatgcc ttgactaagc gctatgcgga ttgaccgctc aaaattcata
     3061 tcgcgccatt ctttccactc ttccgacaaa ttcggtcgtt cgtcaggtgg aatacaaccg
     3121 acggtgattg gacccttacc agcatagcgg cataacattc gtgcgatgac ttgtttaagg
     3181 tcccgcacat tacctggata ttgccgacgt aagagatatt cttcaaccgg cttatcaata
     3241 atgggaatat cttggtttgg acagtttttt tttaggaagt aattcgccaa cggtagaatg
     3301 tcttcagatc gttcgcgcaa cggtggcaaa tggcagatat agcttgcgat ccggtaatac
     3361 agatcagcac ggaatttccc gcgctggact tgtgttgcga gatcgcggtt ggtcgcgcaa
     3421 atcagccgga atgcagtacg ttgccaggca ttgccaccga cgcgtttgta agtttgttct
     3481 tggattaccc gcaaaagctg tgtttgcatt gttagggata attcccctac ttcatctaga
     3541 aaaagtgtgc caccatttgc gagtgcgaat acgccatccc gtgaagaggt ggcgcctgtg
     3601 taggctcccc gttcatgtcc aaaaaattcg ctgcccgata attccggcac gatcgtggtg
     3661 caatctagaa tgataagctc gcctttctca gaacgattgt cgagcgtgtg aatcagatta
     3721 gccagcaatt ccttgccggt accactttct ccaaggacta ataccgagac attggtaaaa
     3781 tgcgcgacct cgatgacttg ccgtagtatg gtttgcaacg tttggctttt accaactaaa
     3841 gcattttgga ctattggaga gttgagtaga gaactgatgg attcccaacg gcggaggcga
     3901 gcggtgatct catgaataat tagattatct tttgtacaaa ccagcacgtc tgaggcgcct
     3961 gcttccatca actgccagat tgcttctcca gaaattgctt cttggcaaca agcaaggacc
     4021 tgcacctgac cgtggttgct taattcatac aatttgttaa gtagttctgg ggtgacttta
     4081 ttgctataga taattcctga ataaggacaa ggagtgcaat aatcttgtat cgcaatgtgc
     4141 tgttgggtta agaggtgcaa aaatcttcga aaggtttcct ctccgtattc accaatgccg
     4201 aaaacccaga ttgagataga ctgagatgta tccattgcga aaagtatcca caaagtattg
     4261 agcaacgagc cagaaataaa ttaggaactg gagttgtccc attttcttgg gtctgttgac
     4321 ttaacatggg catacataga gataatgcgt gttagtgaac aagcttccgt attgcttttc
     4381 agcttgttat atcgtgtacg attgctcgat accgttttag ctgggcaaag atgctgccca
     4441 cgcaaatacc gctatctata ttgaacgaag cccaatccct atctgcgtta ttcgccagcg
     4501 tgttactttt ctggcatcac agatttaact tttttactat cggttgtcgt atgtgcttgc
     4561 tgtgatagcc tttatcgaca accgtcgctt ctccagtaga aaatctagag atcacaaaca
     4621 agtagtgcag atcaatcagt gattacttaa cggatgattt caaatttaac tagtaaagcg
     4681 aaattatcaa ctctatgaaa atgatcaata gcttgttgaa gcaggttttt ctttcccttc
     4741 cacgttgaag aactcctcac cgaacctcaa tcggcacacg gtgcaacgca aatgcgtttt
     4801 ataaatgata taggaatcaa aaattataaa ctcctatttt tatatagaat aattatctct
     4861 aattgtcaat agattttagc caatcattac atttgtgaat ttattcaagt ttggtaagat
     4921 gacagctaaa tcggttaact aacttaaaaa aatgggggat accatgaaga ttgaatctgt
     4981 tgccatttca aattggcgtt cgatcaagta tctcaaaatc gcgttccagg atctgatgat
     5041 cttcactgac tagagcaatc acggcaaatc aaacatactt tccactttgc tgttcttttt
     5101 cggagagatc aagccccaaa acttggactt caaccgaagt attcatagtt ggtgtacgga
     5161 taacaagacg cccactcatc attctatcta ccgagcctgt gcgaaaagct gcgtaagtcc
     5221 ttgagctcag atattacgaa gatcaaagga atcaaactca cttgtttctt ctttgattct
     5281 gatgattccc actttgatga ttttttgata atcctatgat gtctttttgt gctgtcgatc
     5341 catttcagct aattttccaa gtttttcagc gatcttaatc tctaatccgc gtagtgttgg
     5401 atgataaaaa gtaaccggtt ttagcgtttc cgggaaataa ttc
//
LOCUS       DAOQKQ010000170         5397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_8113_length_5397_cov_4.590790, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000170 DAOQKQ010000000
VERSION     DAOQKQ010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8113_length_5397_cov_4.590790"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1929)
                     /locus_tag="PKZ49_10340"
     CDS             complement(<1..1929)
                     /locus_tag="PKZ49_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37004.1"
                     /translation="MAWGRLALIQAIGESEATLQKVAEALGFESKFARQPQMPEQAPI
                     TPDPGGDSAPTMLTTEALPTEKPHAFFLRVNRMETLSPHNVENRGYPYYLDDPALKLD
                     ATQPSQGTYRFAPPPPLLSMSRLAPLLHQGLGQIKTTSRIDFSRMVRHIAQGKFIRRL
                     PRIKQQRWPQQLQIIVDPHVSLEPYWSDFAFIITALKKRLGTERVTALRFDEATLSES
                     QMQATYWPTQPQDRWFVWQLSASDVPLLILSDLGMVTSDANVRYQWRQWITQLRSHAG
                     PILTLSPALQSPQGRQLCALIKPHPLHDRVRLPRHPARKGFELAQPKAETLSNVLTWL
                     SILPITDTGLLRRLRTEMQWGGSELESLIWNHACFSQTSLGLRMPDSSEAQQYRAKYQ
                     QQFAQTPQAQRFWQIVHDHHAQGFEGLRQLERLSRSISEHEHDDAAHCYFQRVCATIR
                     QTSPVSAHAQALQAQCRTVLLSLPDHLFLSDQQDVAYALYAMAYQQEIQAGQWPESLK
                     PGFEPARLQWLIDQEQSQQRLQWWVLQTSAQGYFVCQPGSPETTSPQLPSAVLESLSA
                     IPPTYQVLSGSTLSRRGIVRPAQVFSAADAPLVVETSMQRVELEAIQKPAWALRIKAN
                     RSGLAVDLPIWQGKSVTAP"
     gene            complement(1935..2957)
                     /locus_tag="PKZ49_10345"
     CDS             complement(1935..2957)
                     /locus_tag="PKZ49_10345"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HND37005.1"
                     /translation="MTQFLKLEHFLPPNFILSKPDKQLYHIFDEADCYALWAAYAAGR
                     PLLVRGKPGTGKSQMAKAIAEQLGWAFVSEVIRGSTELSDLHWHFDAIGRLSEAQAMS
                     FKPQGSTDDWENRANPLRFLSPGAFWWAYDWQSAEQQYQACMTKLHPAPEPEQPESLA
                     ASQQPHGVVLLIDEIDKAEPDLPNGLLETLGQYQFTVPYLNQQAAQSELKNPIQAEPA
                     RLLIVITTNEERELPSAFVRRCLVHTLKMEESAEQINGIEKRVAWLIARAQLHFGSQI
                     SEDVYRKAAELLWQDRTSGNYLRYPPGLAEYIDLLKALRGLEPGEQARRLEKIASYTL
                     KKEVTE"
     gene            complement(2970..4436)
                     /locus_tag="PKZ49_10350"
     CDS             complement(2970..4436)
                     /locus_tag="PKZ49_10350"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HND37006.1"
                     /translation="MGEQNTTIKELAGRVPIQAYLSYRHCEETKQARDLLKKHCEAYK
                     IELIYDEEQLKIGDSITAFMDEIASAQCIFIFLTETYFDSAFTLYELIKINECCRDRK
                     TNKPFIVPIKISKSIHGFSASDAKNGHWLGDKNQKCRDDLTEKLGSQVEDHDQAWQRI
                     AEAFPEMLNPFLDKIYPSVEEDASVLETRVNNVYAEIATVIQKSKDKLTQDIKAKITR
                     LLNNRKPIFLNQLSSDLGFASSDNETIARYLAENGDVKGTIKIMTQTLKKQREVLRSV
                     NLEKWRESHAIAEQLCGWLLIKTIDPVWWFNHEFKASKIGKTFASDIFLEERAFVEII
                     ISRDLFQPAKYKRLAETGKVQPFTDNLSIDFIFDANVGALEESFLINLYRDLFKSTGA
                     EGTTAQLKEDIKVQLDTIITEDRKCIYYVTNEATMMSLKSIQGIEEWLQQLEGKLRFI
                     YLPISDEKVLKQAQSPYPEQKALLFQIADLLKIRDEMI"
     gene            4699..>5397
                     /locus_tag="PKZ49_10355"
     CDS             4699..>5397
                     /locus_tag="PKZ49_10355"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HND37007.1"
                     /translation="MNIHGAADKMKALFLSGMTTSMLKLADIPFFNALSPHTLAHLQA
                     TIPVRHYKQGSVIARAGDKGQFFQAIAEGAVWVQQADRYHRKSGIVLGPGQVFGEMSL
                     FSGMPISATLVASKDTKTYCFNSNQFLHLLESVPQLHVNLTNLLIERLRRRTHHEGYA
                     PNLVVLAYETESTLSQKLVERLIDGIHHYSAGSRLFSGLQNPISDAADPELHSKFNAW
                     RESAPNGQLLVLRVR"
BASE COUNT         1303 a         1277 c         1273 g         1544 t
ORIGIN      
        1 aggtgcagtg actgattttc cttgccaaat cggtaaatca accgccaacc ctgaacgatt
       61 ggctttgatg cgtaatgccc aggctggctt ttgaatcgct tcgagttcga cccgttgcat
      121 actggtttcc acaaccagtg gtgcatcagc cgcagagaac acttgcgctg gacgtacaat
      181 accacgccgt gatagtgtgc tgcccgataa aacctgatag gtcggtggaa tggcggataa
      241 tgattccaac accgcgctgg gtaattgcgg tgaggttgtt tctggtgaac cgggttggca
      301 aacaaaatag ccttgtgcgc tggtttgtaa tacccaccat tgcaaccgct gctgggattg
      361 ctcttgatcg atcagccatt gcaagcgtgc gggttcaaaa ccgggtttca acgattctgg
      421 ccactgccct gcttggattt cttgttgata cgccatggca tataacgcat aagcaacgtc
      481 ctgttgatca ctcaggaaca gatgatcggg tagcgacagg agcacagtgc ggcactgcgc
      541 ttgcaaagct tgcgcgtgcg ctgaaacggg tgaggtttgc cgtattgtgg cacacacgcg
      601 ttggaaataa caatgggctg catcatcgtg ctcatgctcg ctaatactac gacttaacct
      661 ttccaactgg cgtagtcctt caaagccttg cgcatggtga tcgtgcacga tctgccagaa
      721 gcgctgagcc tggggtgtct gggcaaattg ctgctgatac ttggcgcggt attgctgtgc
      781 ttcactcgaa tccggcatgc gcaaacccaa gctggtctgg ctgaaacaag catggttcca
      841 aatcaagctt tccaattcgc tgccacccca ttgcatctcg gtacgcaagc gccgcaagag
      901 acccgtatcg gtaatcggta gtatcgacaa ccacgtcagt acgttgctta aagtttctgc
      961 tttcggttgt gcgagctcga atccttttcg ggcggggtga cgcggtaaac gcacgcgatc
     1021 atgcaaggga tgcggtttaa taagtgcgca taactgccgg ccttgcgggg attgcagcgc
     1081 gggactcagc gtgagtatcg gccctgcatg cgaacggagt tgtgttatcc attgccgcca
     1141 ctgatagcgc acattggcat cgcttgttac catgcccaga tcgctcaaaa tcaacaatgg
     1201 cacgtcggac gccgacaatt gccagacaaa ccaacgatcc tgcggttgcg tcggccagta
     1261 agttgcctgc atctgcgatt cactcagcgt cgcttcatca aagcgcaacg cagtgacgcg
     1321 ctcggtaccc aatcgtttct taagcgcggt gataatgaaa gcaaaatcag accagtaggg
     1381 ttctaaactc acgtggggat cgacaatgat ttgcaactgt tgcggccaac gctgctgctt
     1441 gatgcgcggc aatcgacgga tgaatttacc ttgtgcgatg tgacgcacca tccgtgagaa
     1501 atcgattcga ctggttgttt tgatttgccc cagaccctga tgtaacaaag gcgccaagcg
     1561 tgacatcgat aataacgggg gcggtggcgc gaaacgatac gttccttgcg atggctgggt
     1621 cgcatcgagt tttaacgcag gatcatccag atagtacgga tagccacgat tttccacgtt
     1681 atgcggcgac agcgtttcca tgcggttaac gcgcaaaaag aaggcatggg gtttttccgt
     1741 cggcagtgct tcggttgtta gcatggttgg ggcgctatct ccccctggat ctggtgtgat
     1801 tggcgcttgc tcaggcattt gcggttggcg tgcaaacttg gactcaaaac ccagggcttc
     1861 agctactttc tgtaatgtgg cttcactctc accgatcgct tgtatgagcg ctagtcgccc
     1921 ccatgccatg gcgtttactc cgtcacttct tttttcagcg tgtaactcgc gattttctct
     1981 agacggcgag cttgctcgcc tggttccagt ccacgtaacg ctttgagcaa atcgatgtat
     2041 tcggcgagcc ccggtggata acgtaaataa ttccctgagg tgcgatcttg ccacaatagc
     2101 tcagccgctt tgcgatatac atcctcactg atttgtgacc caaaatgtaa ttgggcacgc
     2161 gcgatgagcc aggctactcg cttttcaatg ccattgatct gttccgctga ttcttccatt
     2221 ttcaacgtat gaaccaaaca acggcgcaca aaagcgctcg gtaattcacg ttcttcattg
     2281 gtggtgatca caatcagtaa tcgcgctggc tcagcttgga tcgggttctt aagttcgctt
     2341 tgagcagctt gttgattgag atacggcacg gtgaattggt attgcccaag ggtttccagc
     2401 aagccattgg gtagatccgg ttcggcttta tcgatttcat cgatcaacag cactacgcca
     2461 tggggctgct gggaagccgc taagctttct ggctgctccg gctccggtgc agggtgcaat
     2521 ttagtcatac aggcttgata ctgttgttcg gctgattgcc aatcataggc ccaccagaat
     2581 gcaccgggag acaagaaacg cagcgggttg gcgcgatttt cccagtcatc ggtgcttccc
     2641 tgcggtttga aactcatcgc ttgcgcttca ctcagacgac caatggcatc gaaatgccag
     2701 tgcagatcgc tcaattcggt gctaccgcga atcacttcac tgacaaaggc ccaaccgagc
     2761 tgttcggcaa tggctttggc catttggctc ttccctgtgc cgggcttgcc gcgaactagc
     2821 agtggtcgcc ctgccgcata ggctgcccat aacgcatagc aatccgcttc atcaaagata
     2881 tggtaaagct gtttgtcggg tttcgatagg atgaaattag gaggtaggaa gtgttcgagt
     2941 ttgagaaatt gggtcatagg tttaaatttt taaatcattt catctctgat ttttaataga
     3001 tcggctattt gaaaaagcaa tgctttctgc tcaggataag gcgattgcgc ttgcttcaat
     3061 actttttcat cggatattgg caaatatata aaccgtagtt ttccttccaa ctgttgcaac
     3121 cactcttcaa tcccctgaat agattttaaa ctcatcatcg ttgcttcatt ggttacatag
     3181 taaatacatt tacgatcttc agtgataatc gtgtcaagct gaaccttaat atcctctttt
     3241 aattgtgctg tagtcccttc cgcaccggta gacttaaaca aatctcgata cagatttatc
     3301 aaaaaactct cttctaatgc gccaacgttc gcatcgaaaa taaaatctat actgaggttg
     3361 tcagtaaacg gttgtacctt ccctgtttca gctaacctct tatatttcgc gggctgaaat
     3421 aaatctctgg aaataataat ttcaacaaaa gccctctctt ccaagaaaat atcgctcgca
     3481 aaagttttac cgattttgct cgctttgaat tcgtgattaa accaccaaac tggatcaatc
     3541 gttttaatca gtaaccatcc acacaattgt tcagcaatcg catgactttc acgccatttt
     3601 tcgagattaa cactccgtag gacctctctt tgctttttaa gcgtttgggt cattatctta
     3661 attgtgcctt ttacgtctcc attctcggcc agatatcgag caatcgtttc attatcgctg
     3721 ctggcgaatc ctaaatccga actgagctga ttcaggaaaa taggctttct gttatttaga
     3781 agccgtgtaa tttttgcttt aatgtcttga gtgagtttat cttttgattt ttgtattacc
     3841 gttgcaatct ctgcatagac attattaaca cgggtttcca gaaccgatgc atcctcttcc
     3901 actgaaggat atattttgtc taaaaacggg ttaagcattt ctggaaatgc ctcagcaatt
     3961 cgttgccatg cttgatcatg atcttctact tgtgatccaa gtttttcagt tagatcatca
     4021 cggcactttt gatttttgtc gcctaaccag tgaccatttt tcgcatctga tgctgaaaag
     4081 ccatgtattg attttgatat ttttattggt acgatgaatg gcttattcgt cttacgatcc
     4141 ctgcagcatt cattgatttt aattagttcg tacaaggtaa acgcagaatc aaaataggtt
     4201 tcagtgagaa aaataaatat acattgtgct gacgctattt cgtccatgaa agccgtaata
     4261 ctatcgccga tcttgagctg ctcttcatca taaattaatt cgattttgta ggcttcgcag
     4321 tgtttcttta ataaatcacg ggcttgtttg gtttcttcgc agtggcggta ggataaataa
     4381 gcttgaatgg gcactctgcc cgctaattct ttgatagttg tgttttgctc acccatgttt
     4441 tgcctgtttt aatacaacga tcatctgata gtgaaaattc taccaccgtc attgcacaaa
     4501 agtggggttg cttgcaccaa tatgtcataa gacaatttag gcatagatag ccaatttggc
     4561 tatttttcgc agtgtacggt tttagtttca tcaaaaaata cagggtgttc gtttcgttat
     4621 cagtgcgttt gccaagagaa ctgttagaat acaatgagga gtaaagcgtt gtaagggctg
     4681 cggcaagcgc tggaatcgat gaatattcac ggtgctgctg acaaaatgaa agctttgttt
     4741 ctttcaggga tgacgacttc catgctaaaa cttgcggata ttccattttt caatgcgtta
     4801 tcgcctcata cgttagcgca tctacaggcc acgattccag tgcgccatta caagcaaggc
     4861 agtgtcattg cacgcgctgg agataaaggc caatttttcc aggcgattgc cgaaggtgcc
     4921 gtgtgggtac aacaagcaga tcgctatcat cgcaaaagtg gcattgtctt agggccgggt
     4981 caggtatttg gtgagatgtc gctgttttca ggcatgccta tttctgcgac gctagtcgct
     5041 tccaaagata ctaaaaccta ttgtttcaac agtaatcaat ttctgcattt gctggaaagc
     5101 gtaccgcaac tacatgtcaa tctaacgaat ttattaatcg agcgcttacg gcggcgtacc
     5161 catcatgaag gctatgcccc gaacttggtg gtgctggctt atgaaacaga atcgacgctt
     5221 tcgcaaaaat tagtggagcg gttgatcgat ggaattcatc actattcagc gggctcaagg
     5281 ctattctcag gcttgcaaaa cccaatcagc gacgccgctg atcctgaatt acactccaag
     5341 tttaatgctt ggcgtgagtc tgcccccaat ggtcaattac tcgtgctcag agttcgc
//
LOCUS       DAOQKQ010000171         5344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_8240_length_5344_cov_7.350539, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000171 DAOQKQ010000000
VERSION     DAOQKQ010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8240_length_5344_cov_7.350539"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..502)
                     /locus_tag="PKZ49_10360"
     CDS             complement(137..502)
                     /locus_tag="PKZ49_10360"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HND37008.1"
                     /translation="MARAFSEIAFTDSVNSMQTGMYGSHQAYSKFDLAEERRDYLGEH
                     EIAFLAERDSFYMSSVGENGWPYIQHREGSKVFLKVLDEKTIGFADYRGSKQYISIGN
                     LSVDNRVSLFIMDSSATCR"
     gene            complement(537..1514)
                     /locus_tag="PKZ49_10365"
     CDS             complement(537..1514)
                     /locus_tag="PKZ49_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HND37009.1"
                     /translation="MMIKTILLSVITLGLLSATPLVTAAPANNEHAAGKPTQLNYRTA
                     NIDGVEVFYREAGPKAAPVLLLLHGFPTSSHMFRNLIPQLSDRYHVIAPDYPGFGQSA
                     MPDRDKFAYTFDNYAQIVDKLMQQLGVNRYAIYVMDYGAPVGFRLASSHPERVTALIV
                     QNGNAYNEGLEQFWDPINAYWETGGSTEREAIRWLTSIKATKWQYTNGVNDTSLVSPD
                     TWTIDQALLDRPGNAEIQLDLFYDYRTNIPLYPQWQAYFRKHKPATLVVWGKNDAIFV
                     AAGAAPYKRDLPDADIHLLDTGHFALETHGSEIAKLIREFLDKRLKPKG"
     gene            complement(1542..1754)
                     /locus_tag="PKZ49_10370"
     CDS             complement(1542..1754)
                     /locus_tag="PKZ49_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37010.1"
                     /translation="MNRIAIPTPDQTPAASLPLLDAVKATGYNQAEIVEIVLHVALNT
                     LTNYINEIAQTEIDFPVISPATRATA"
     gene            1893..2792
                     /locus_tag="PKZ49_10375"
     CDS             1893..2792
                     /locus_tag="PKZ49_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HND37011.1"
                     /translation="MDRLHLMTVFVAVAEEESFAAGARRLGMSPPAVTRAVSALEDHL
                     GVKLLNRTTRYVRVTDAGNRYLDDARRIIGEIDEADDAAAGINAAPRGHLAVTAPVMF
                     GKLFVLPGIVDYLKRYPDMEVSAVFLDRVVNLLEEGFDVGIRIAELPDSSMRAIRVGQ
                     VRRILCASPDYLERNGTPSNPTELSSHNIIASSGVSFTNEWKFNPELELSTVRVKPKL
                     TVSSNDAAIEAACQGIGITRLLSYQVVPYLTSGQLKIILRDFEPASIPIHVLHREGRY
                     TSAKIRSFVDLIVAKLKEDDALS"
     gene            2872..3129
                     /locus_tag="PKZ49_10380"
     CDS             2872..3129
                     /locus_tag="PKZ49_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37012.1"
                     /translation="MSALLLNSRRLNCIYSMAEYFALNKSVIDLALKNIGKSLGNWQL
                     TAKRHQLRSDEIKRMAPSFEHENSQRLISIATKTISRSRSS"
     gene            3143..3529
                     /locus_tag="PKZ49_10385"
     CDS             3143..3529
                     /locus_tag="PKZ49_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37013.1"
                     /translation="MCGGIEYQSEKIYFPQPGARLPVRLRDGNVTWVTWGRRKDEVIG
                     KFPNGGGWARLNSIKSGKWKPWYPRPVSITANQFMEKDHDNQSHWVPLDTRMAIQGLL
                     AKRDDEMRVYVVTINAPPEYAWIHDC"
     gene            complement(3633..>5344)
                     /locus_tag="PKZ49_10390"
     CDS             complement(3633..>5344)
                     /locus_tag="PKZ49_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2326 domain-containing protein"
                     /protein_id="HND37014.1"
                     /translation="IRLAENRRKDTHNLGKSTLGRLIDFALLMNRDPRFFLFKHLDIF
                     KEFIFYLEVELEDASFLTIRRGVDEATRISFKKHQTGHQDFSTLPSPEWDHSDVPFER
                     AIDLLDSLLDWRVLKPWSYRNGLGYLLRTQDDYQDVFQLRKFAGKHAYWKPFLAHILG
                     FDAELISEHYDKEKQLSEKQETAETIKKELGGSIEDISKVEGLLLLKQKEAEKKQELL
                     DAFDFRMQDKTHTKYLVDGIDERIASLNGERYSLTQSKKKIMASLEDDGILFNPDEAQ
                     RLFAEAGVLFQGQIKKDFQQLIAFNRAITDERRIYLQEECAEVESELKRVNAELNTLG
                     KKRSETLAFLSGTDVFNKYKQVSDEMVTLRADITSLERQRGFLHRLQELRTTIRTLSE
                     ELSHLQTRIEANVEEQNSDQKSLFSTIRLFFSEIVEEVIDRKALLSVSPNKDGHLDYT
                     AEILDEAGNATSANQGHTYRKLLCIAFDLAVLRAHIDDKFPRFVYHDGIFESLDDRKK
                     ENLLSVIRRYADLGLQPMITLIDSDLPIRSENELPVFDSEEIVVLLHDENAQGRLFKL
                     KSW"
BASE COUNT         1417 a         1173 c         1265 g         1489 t
ORIGIN      
        1 ggcctaatct gaatcaattg agcttaaccg ccactctcgg atcacactgc ggtgattcgc
       61 gttggaatcc ggcgggattg tggaaaaatc actgggttct cccgccggct ccgaagtata
      121 ggtatacagc aggccgttac cggcatgttg cggatgaatc catgatgaac agcgagacgc
      181 ggttatcgac actcaggtta ccgatgctga tatattgctt actacctcgg tagtcggcaa
      241 agcctatggt tttttcatcc aacaccttca aaaaaacttt agagccttca cgatgctgaa
      301 tatagggcca gccgttttca ccaaccgaac tcatataaaa gctgtcgcgt tcagccagga
      361 aggcaatttc gtgttcgcct aagtaatccc ggcgttcctc ggcaaggtcg aatttcgaat
      421 aagcctgatg cgagccatac atacctgttt gcattgaatt cactgagtcg gtgaatgcaa
      481 tttctgaaaa tgcacgagcc atggatgtgt ctccttgtcg gcgggttatg tcgattttaa
      541 cccttgggct tgaggcgctt gtcaaggaac tcacggatta gtttggcgat ctccgagccg
      601 tgggtttcaa gggcgaaatg tccggtatca agcagatgga tgtccgcatc gggcaaatca
      661 cgcttataag gtgcggcgcc cgcagcaaca aaaatcgcat cattcttgcc ccaaaccacg
      721 agtgtagcgg gtttgtgctt gcggaaataa gcctgccatt ggggatacaa gggtatgttt
      781 gtccgataat cgtagaacag gtcaagctgg atttcggcat tgcctggacg gtcgagcagt
      841 gcctggtcta tcgtccacgt atcagggctg accaacgagg tgtcgttcac gccattggtg
      901 tattgccact tggtggcctt aatcgaggtc agccaacgaa tggcttcacg ttcggtcgat
      961 ccaccagttt cccaatacgc attgatcgga tcccagaatt gttcaaggcc ttcattatat
     1021 gcatttccat tttggacgat gagcgccgtc actcgttctg ggtggcttga tgccagtcgg
     1081 aagcccactg gcgcaccgta atccattaca tagatggcgt agcggtttac gccgagttgc
     1141 tgcatcagtt tgtcaacgat ttgcgcgtaa ttgtcaaagg tgtacgcaaa cttatcccga
     1201 tctggcatag cgctttgccc gaaaccgggg taatctggag caatcacatg gtatctgtcg
     1261 gacaattgcg ggataaggtt acgaaacatg tgcgacgatg ttggaaagcc gtggagcagt
     1321 agcagcacgg gtgcagcttt tggccctgcc tcacggtaga atacctccac cccatcaatg
     1381 ttggcggtgc gatagttcaa ttgtgttggt ttgccagcgg catgctcgtt attagcaggg
     1441 gcagcagtta ctaaaggcgt tgcgctaagc agccctaagg ttataacgct taacaggatt
     1501 gttttaatca tcatggcagt ctcctaaaat taacaaaagc ctcacgcagt agcgcgtgtg
     1561 gcagggctta tgacagggaa gtcaatctcg gtctgggcaa tttcgttgat atagttggtc
     1621 aaggtattca aggcaacatg caatacaatc tcaacaatct cagcttgatt gtaaccagtc
     1681 gctttgacgg catccagcag tggtaaggaa gcggcaggag tttggtcagg ggttgggata
     1741 gcaattctgt tcatagcatt tctccaggtt aaaagtagtt gtttaaattt gtgccgcaca
     1801 ctgtgttacg gacggttaca ctttctcaca ttccacttgc cattagaata atcgaatagc
     1861 acaatacatt tttccgtaaa gtggaataaa caatggacag gttacattta atgaccgtat
     1921 ttgtggcggt tgctgaggaa gagagctttg cagcgggagc aaggcggctt ggtatgtcac
     1981 cgcctgcggt aacacgtgcc gtttcagcat tggaagacca tcttggcgtg aaactgctta
     2041 acaggacgac gcgttatgtg cgcgtgaccg atgcaggaaa ccgctatctt gatgacgccc
     2101 ggcgcatcat tggcgaaata gatgaggcgg atgatgccgc tgcaggcatt aatgcagcac
     2161 ctcgtggcca tttggctgtt acggctccgg tcatgttcgg taagctattt gtgcttcccg
     2221 gtattgttga ttatttaaaa cgatatccag atatggaagt ctcggctgtt ttcctagatc
     2281 gtgttgtcaa tttgcttgag gaaggttttg atgtcggaat ccggattgcc gaattaccgg
     2341 attccagtat gcgcgcgatt cgggtcggtc aagttcggcg tatactctgt gcgtcacccg
     2401 attatcttga aaggaacggt actccaagca atccaactga gttgtcgagt cacaacatca
     2461 tcgcatcttc cggcgtaagc ttcaccaatg agtggaaatt caacccagaa ttggagttat
     2521 ccaccgtacg ggtcaagcct aagctgacgg taagcagcaa tgacgcggca atcgaagccg
     2581 cttgccaagg cattgggatc acgcgattgt tatcctatca agttgtccca tacctgactt
     2641 ctgggcaact taagattata ttgcgtgatt ttgagcctgc atcaataccc atccatgtcc
     2701 tacaccgtga aggacgctat acctcagcaa aaatacggtc ttttgtggac ttaattgtag
     2761 ccaagttgaa agaagacgat gcattaagtt gagtttaagt aaatggctat ctatttgaag
     2821 ctgttaatca ccactagcgc gatatgatca cttgaatacc ctgcgcgccc tatgtctgca
     2881 ttattactca attcaaggcg cttaaattgt atatatagta tggcagaata ctttgcgtta
     2941 aataaatcag tgatagatct tgcattaaaa aatatcggaa aaagtctggg taattggcaa
     3001 ctgaccgcaa aacgtcatca attgcgcagc gatgaaatca aacgtatggc accatccttt
     3061 gagcatgaaa acagtcaacg gttaatttca atagccacaa aaacaatttc aaggtcacgc
     3121 agtagctaat ggtagggtta ttatgtgtgg cggaattgaa tatcagagtg aaaagatata
     3181 tttcccgcaa ccgggtgcgc gattgcctgt gcgtttgcga gatggcaatg ttacctgggt
     3241 aacgtggggc aggcgcaagg atgaagtcat cggaaaattt ccgaatggcg gcggctgggc
     3301 acgactgaat tccatcaaaa gcggcaaatg gaaaccctgg tatccaagac cggtttcgat
     3361 taccgctaac cagttcatgg aaaaagacca tgacaaccaa tcccactggg tacccttgga
     3421 tacaagaatg gcgattcaag gactattagc aaaaagagat gatgaaatgc gggtttatgt
     3481 ggtgactatc aacgcgccac ctgaatatgc ttggatacat gactgctgac cgagactggt
     3541 tcgactgaca gtgtttggtg ctctacaatg agctatccgg cattaaatca ggctatacga
     3601 tctgatctga actagcacac ctaaaatata aatcaccaag actttaactt gaataagcgt
     3661 ccttgggcat tctcgtcatg taaaagtacc actatttctt cgctatcgaa tacgggtagt
     3721 tcattttctg accttattgg caagtcagaa tcaatcagag taatcattgg ttgcagacca
     3781 agatcagcgt aacgtcgaat aacactaaga agattctctt ttttcctgtc atccagcgat
     3841 tcaaatatgc catcgtgata tacaaaacga ggaaatttgt catctatatg tgctctgagt
     3901 actgccaaat caaatgcgat gcacagtagc ttacggtaag tatgaccttg atttgcactt
     3961 gtggcattac cagcttcgtc aaggatctcg gcagtgtaat ctaagtgacc gtccttattc
     4021 ggcgatacgc tgagcagcgc ttttcggtcg atcacctctt caacaatttc actgaagaaa
     4081 agccgaattg ttgagaaaag gcttttttga tcagaattct gctcctcaac gtttgcctct
     4141 atgcgtgttt gtagatgact tagctcttca cttaatgtac gtatagtagt gcgtagttct
     4201 tgcaatcggt gcaggaagcc acgttgacgc tcaagagaag tgatatccgc gcgaagtgtt
     4261 accatttcat cagatacttg cttatattta ttgaagacat cagttccact aagaaatgcc
     4321 aatgtttccg agcgcttttt cccaagagta tttaattcgg cattgacacg ttttagttct
     4381 gactcaactt cggcacattc ctcttgaaga taaatacgac gctcgtcagt gatggcgcga
     4441 ttgaatgcga ttagttgctg aaaatctttt ttaatctgac cttggaagag cactccagcc
     4501 tcggcgaata aacgctgagc ttcatcaggg ttaaatagaa ttccgtcatc ctctaaggat
     4561 gccataattt ttttcttact ttgagttagt gaataacgct ctccatttaa gctagctatg
     4621 cgctcatcaa tgccatcgac tagatatttt gtatgagttt tgtcttgcat gcgaaaatcg
     4681 aaagcatcaa gcaattcctg ttttttttca gcctcttttt gctttaatag caagagacct
     4741 tcaaccttac tgatgtcttc aattgaaccg ccgagctcct ttttgatagt ttcggctgtt
     4801 tcctgttttt ctgatagttg tttctctttg tcgtaatgtt cggaaatgag ttctgcatca
     4861 aagcctagta tgtgagcaag aaatggcttc cagtaagcgt gttttccagc gaatttacgc
     4921 agttgaaaaa catcttggta gtcatcttga gtacgcagca aataaccaag tccattacga
     4981 tatgaccaag gttttagtac acgccagtca agcaagctat ccaacaagtc aatcgctcgc
     5041 tcaaatggaa cgtccgagtg atcccattct ggcgatggga gtgtagagaa gtcttgatgg
     5101 ccagtttgat gtttcttaaa actaattcgc gttgcttcat caacgccgcg acgtattgtg
     5161 agaaatgatg catcttcaag ctcaacttca agatagaaaa taaattcttt gaaaatgtct
     5221 agatgtttaa aaagaaaaaa cctcggatca cgattcatta agagtgcgaa gtcgatcaat
     5281 cgacctaatg ttgattttcc gagattatga gtatccttcc tgcggttttc agccagtcga
     5341 attt
//
LOCUS       DAOQKQ010000172         5337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_8255_length_5337_cov_5.638016, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000172 DAOQKQ010000000
VERSION     DAOQKQ010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8255_length_5337_cov_5.638016"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..481
                     /locus_tag="PKZ49_10395"
     CDS             89..481
                     /locus_tag="PKZ49_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37015.1"
                     /translation="MNFETEMRARFGGFWNSLLLLLIIIIFLRFSAIELIAEGLGAKL
                     SLSLKLFFGFALFGVVLALFKMLFSSSNPPYYRLPASVNIAWGLKRGLVIGGILMLII
                     GILHHDNFLGVLQPLLEKLVGKIIGIFG"
     gene            complement(554..997)
                     /locus_tag="PKZ49_10400"
     CDS             complement(554..997)
                     /locus_tag="PKZ49_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37016.1"
                     /translation="MDELTIHLKSSQQLTLMFSVVHVACIVIIALLDGSILLKASGML
                     LLVLSLIYYIRLYALLQSPNAIVSFYLSEHSTCITQTRSGQQLHWHIQGSSFVTPYLT
                     VLSLKSNQSFFDRSIVIFPDSINAEQFRQLRVWLRWKWKDTNSLE"
     gene            1023..2045
                     /locus_tag="PKZ49_10405"
     CDS             1023..2045
                     /locus_tag="PKZ49_10405"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HND37017.1"
                     /translation="MNELNQSINENLLDLQKIPIEKGVVDLSDFGIIRFTGEDTLTFL
                     QGQLSCDVLKVTPTQAQHGCYCSPKGRILANFILAADHTGEAWYMQMPRAILDKIVKR
                     LSMYVLRAKVKLVDYSAESIRLGIVGSQAHSLLEKSLPTLTMPESRLSVAQVAGITVI
                     CLGPNRFEMIAPFEQGNQLKTYFDKATSWVNLSYWQWLDIQAGIPTISTETQEQFIPQ
                     MVNMDLIGGISFQKGCYPGQEIVARTQYLGKLKRRMTLIRIETTETVAAGDPLYSESL
                     GDQACGMIVNAVRIAEGIFEALAVIPIDVWNSSIQWKSLQGPIIHRLTLPYSLPEKLQ
                     IDLTDH"
     gene            complement(2281..2721)
                     /gene="dksA"
                     /locus_tag="PKZ49_10410"
     CDS             complement(2281..2721)
                     /gene="dksA"
                     /locus_tag="PKZ49_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HND37018.1"
                     /translation="MVSRKKQPDSSKKSEKQEIHEVPAGEYMCAAQLAFFRERLESEK
                     QALLENAQKTLESIQNTEPGADPNDRASTEEEYLLEFKVRDRERKLLRKIEDALKRIE
                     DGTYGWCEETGEPIGIPRLLARPTASLCIEAQERHEQTDKLYEH"
     gene            3106..3852
                     /locus_tag="PKZ49_10415"
     CDS             3106..3852
                     /locus_tag="PKZ49_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HND37019.1"
                     /translation="MLSVHNLNLQQSDRVLCGPLNFTIKPGEFWIILGENGCGKSTLL
                     QTMSRLRMENSGTITLQEKPLEKIHQPMLAQQIGILLQEETPEFWGKVREYILLGRHP
                     YKKSLSGWNHTDHTIVDQILKTHALESLANRFFNTLSGGERQRVRIAQLLAQSPQYFL
                     LDEPLQHLDIRHQLHIMTLFKNISEQNCAVVMVLHDINWLNHFGDHILMMFSDGNALS
                     GPKEMMLTQLNLDKLYQCPIAFFKHYINIP"
     gene            3914..5296
                     /gene="pcnB"
                     /locus_tag="PKZ49_10420"
     CDS             3914..5296
                     /gene="pcnB"
                     /locus_tag="PKZ49_10420"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HND37020.1"
                     /translation="MIRKFIKRFFTRAVTPSSQDVLPPTLCRISRKQHGITRNQISAS
                     ALKVTLALQQAGYSAYIVGGAIRDLLLDIKPKDFDIATNAMPEEVRGLFRRSRIIGRR
                     FRLVHVMFGDEIVEVSTFRANYSADNDAGMMAPNPADPHGRLLHDNIFGSQEEDVLRR
                     DFTMNALLYNPATEEILDYLNGYQDIQARCLRIIGNPEQRYREDPVRMLRAVRLTAKL
                     NVQLDEITAAPIGDLAPLLQNVPSARLFDEILKLLLSGHSLACLIDLRKRGLHHGLLP
                     ILDIVLEQPLGERFISIALKNTDERIQQNKPVSAGFMFAILLWHETLAAWNRRLALDE
                     KPIPALHQAMTETLSTQRAQIAMPRRFDSMIYDIWTLQPRFQSRSGTRPFRLLEHPRF
                     RAAYDFLWLRCESGEIDKEIAEWWHAFQHASPANRESMLIKETPVKKRKRNTRKRKPI
                     VSAESEKNDQ"
BASE COUNT         1600 a         1143 c         1066 g         1528 t
ORIGIN      
        1 accatcattc cttcagaaaa ttgaatctga aaaaaattaa aaaacagtta aatttaaggc
       61 catttcaatt tttcacggga gggaatagat gaacttcgaa acggagatga gagcgcgatt
      121 tgggggcttt tggaattcgc tattgctgtt gctcattatc attatttttt taagattctc
      181 tgcaattgag ttaatagcag aaggactggg tgcaaagtta tcactatcac tcaaattatt
      241 ttttgggttt gcattatttg gggtagtgct cgccttgttc aaaatgttgt tttcttcttc
      301 caatccccct tattatcgtc tgcccgcttc agtcaatatt gcatggggac tcaaacgggg
      361 tttagttatc ggtggtattt taatgctgat tattggcatt ttgcatcatg acaatttttt
      421 aggggtattg cagccattgc ttgaaaagct cgtgggtaaa atcatcggta tatttggtta
      481 gtttggttgt atcatcattt aaacgttgca cgcgcttgtt atgagttagt gtgcaacgtt
      541 atggtgtgtt ggatcattct agggaattag tatctttcca tttccatcgg agccagactc
      601 tgagttggcg gaactgttcg gcgttgatac tatctggaaa aatgacaata ctgcgatcaa
      661 aaaacgattg attcgatttc aaactgagca cggtcagata aggagtcaca aagctactgc
      721 cctggatatg ccagtgtagc tgctgccctg atcgcgtttg ggtgatacaa gtggaatgct
      781 ccgatagata aaacgacacg atggcattgg gtgattgaag caatgcatat agccgaatat
      841 agtaaatcaa gctcagcaca agcaatagca ttccacttgc cttcaacagt atcgaaccat
      901 ccagcagcgc aatgataacg atacaagcca catggacgac cgaaaacatt agagtcaact
      961 gctgggaaga tttgaggtgt atcgttaact catccatcgc attaaattaa aaaattgcag
     1021 taatgaatga actgaatcaa tctattaatg aaaatctgct cgatctacaa aaaataccga
     1081 tagagaaagg ggttgttgat ctttcagact ttgggatcat ccgctttact ggcgaagata
     1141 ctttaacatt tctgcaagga caattgagct gtgatgttct gaaagttacg ccaactcaag
     1201 ctcagcatgg ttgttactgt tcaccgaagg gacgcattct tgccaatttt atccttgcgg
     1261 ctgatcacac aggagaagct tggtacatgc aaatgccacg cgccattcta gacaaaatcg
     1321 ttaaacggct atccatgtat gtgctgcggg ccaaagtaaa attagtggac tatagcgcgg
     1381 aatcaatccg gctgggaatt gtaggtagtc aagcgcattc tttgcttgaa aaaagtttgc
     1441 caacattaac aatgccggag tctcgtttaa gcgtcgcaca agtagcgggt ataaccgtaa
     1501 tctgcctggg acccaatcgc tttgaaatga ttgcaccttt tgagcaaggc aatcagctca
     1561 aaacgtattt tgataaagcg acatcctggg taaatctatc ctattggcag tggctcgata
     1621 tccaagcggg tattccgaca atctcgactg aaactcagga acaatttata ccacaaatgg
     1681 taaacatgga tctcattggt ggaatcagtt ttcagaaagg ttgttatccg ggacaggaaa
     1741 tcgtagctcg cacccaatat ctaggcaaat taaagcgtcg catgacactg atccgcatcg
     1801 aaactactga aacggttgct gccggtgatc ccttatatag tgaatcatta ggtgaccagg
     1861 cctgcggcat gatcgtaaac gctgtaagaa ttgctgaagg tatttttgaa gctttagctg
     1921 tcatcccaat cgatgtatgg aattcgagta tccagtggaa aagcttgcaa ggaccgatta
     1981 tacaccgcct aaccttaccg tattcactac cagaaaaact gcaaattgac ctgaccgatc
     2041 actgacaaac agaaaactaa ccagcgatcg atttactgga aattactacc atttattggg
     2101 aaatgattgc tataaagatt ccctcgatta aaccgtactt agtctttcat gggaaagaaa
     2161 tttttcatga aattcctatt aacgtgagtt ggccccatca gtgtaaaaac tgatggggtt
     2221 ttttattgcg tgatgcaaaa ccaatcttat ctaagcacgt tagtatagct aagacaagta
     2281 tcaatgttca taaagcttat cagtttgctc gtggcgttcc tgggcttcaa tacatagcga
     2341 agcagtgggc cttgccaata accgcggaat accgattggt tcaccagttt cttcacacca
     2401 gccataagtt ccatcttcga ttcgcttaag cgcatcttca atttttctaa gcaatttacg
     2461 ttcacgatcc ctgactttga actctaacaa atactcttct tctgtacttg ctcgatcatt
     2521 aggatctgca ccaggttcag tattttggat cgattcaagg gtcttttgag cattctcaag
     2581 aagcgcttgt ttctcgctct caagtctttc tcgaaaaaaa gcaagctgtg ctgcgcacat
     2641 gtattcacca gctggaactt cgtgaatctc ttgtttttca gattttttag aagaatcagg
     2701 ttgtttttta cgagatacca tattttttag tatttgctta gaatgaaaaa atttcactta
     2761 cacaacataa atgatacaaa actaccaaaa cctcacactt agctaagcta cttcctgagt
     2821 tactattcaa tagcccaaag tttactatta cttactgcaa aacaactaga taccatatat
     2881 cagcctataa aagcaatatt aacaatcttt agaaagatta aaaatactca aataattaag
     2941 cataattttc gaattctata ttttaataag gaattatttc aattaataat tatccatttg
     3001 gataattatt aattgaaaat atttctaaca actgaattga acttcaagta ttcaccattt
     3061 ttccccatac aagctattct ttagcattaa ccattacaat actcaatgct atctgttcat
     3121 aacctcaatc tccaacaatc agacagagta ctctgcgggc cacttaattt tacgatcaaa
     3181 cctggagaat tttggatcat cttgggagag aatggttgcg gaaaatctac actattacag
     3241 acgatgagtc gacttcggat ggaaaatagc ggaactatca cgttacaaga aaaaccgcta
     3301 gaaaaaattc atcagccgat gcttgcgcaa caaattggaa ttttgctaca agaagaaacg
     3361 ccagaatttt ggggaaaggt tagagagtat attctactgg gtcgtcaccc ttacaaaaag
     3421 tcgttatccg gatggaatca tacggatcat acgatagtcg atcaaatcct taaaactcac
     3481 gcattagagt cgcttgccaa tcgatttttc aatacccttt caggcgggga acgtcaacgt
     3541 gttcgtattg ctcagttgtt agcacaatcc cctcaatatt ttttgttaga tgaaccgctg
     3601 cagcacctcg atatacgaca tcaactccat atcatgacct tatttaaaaa catttccgaa
     3661 caaaattgtg cagtggtgat ggttctgcat gatatcaatt ggctaaacca cttcggggat
     3721 cacatcttga tgatgtttag cgatggcaat gcactaagcg gacctaaaga aatgatgttg
     3781 acccagttga atttagataa gctttatcaa tgccctatcg cttttttcaa gcattacatt
     3841 aacatccctt aagtcttcgt tactgacacg ctgctcagtg tataatatcc aatgatactt
     3901 ttagcatctt gtaatgatac gtaaatttat taagcgcttc ttcactcggg cagttactcc
     3961 atcgagccag gatgtcctac ctccaacact ttgtagaatt tcacgtaaac agcatggcat
     4021 aacacggaat cagatcagtg ccagtgcctt aaaagttacc cttgcactcc agcaagctgg
     4081 atattcagcc tacatcgtcg gtggggctat ccgtgatctg ttacttgata ttaagcctaa
     4141 agatttcgat attgcaacga atgcaatgcc cgaagaagtc agaggattat ttcgcagatc
     4201 gcgaatcatt ggaagacgat ttcgcctggt ccatgtgatg tttggcgatg aaatcgttga
     4261 agtatcgaca tttcgtgcaa attatagtgc tgataacgat gcaggcatga tggcacccaa
     4321 ccctgctgac ccccatgggc gcttgctcca tgataatatt ttcggtagcc aagaagaaga
     4381 tgtgctgcgc cgtgacttca cgatgaacgc gctgctctat aatccggcca cagaagaaat
     4441 tcttgattat ttgaatggct atcaagacat tcaagcaagg tgtttacgaa tcattggtaa
     4501 tcctgagcaa cgttaccgag aagatccggt acgcatgcta cgtgccgttc gattaaccgc
     4561 caagcttaac gttcagctcg atgaaataac tgcagcgcca attggtgact tagctcccct
     4621 cctacaaaat gttccttctg cacgtttatt cgatgaaata ttaaaactat tattatctgg
     4681 ccattctttg gcatgcctga tcgatctgcg caaacgtggc ctacaccatg gtttactgcc
     4741 catactcgac atcgtcctag aacaaccgct tggcgagcgc tttatttcga tagccctcaa
     4801 aaatacggat gaaagaatcc aacaaaacaa gccagtttcc gctggattca tgtttgcgat
     4861 cctactctgg catgaaacac tcgctgcttg gaatcgacga ttagcactgg atgaaaaacc
     4921 gataccggca cttcatcaag ccatgaccga aacgctatcg acacaacgcg cccagatcgc
     4981 aatgccgaga cggtttgatt ccatgatata cgatatctgg acattgcaac cgcgcttcca
     5041 gtcacgctca ggaaccaggc cattccgtct attagagcat ccacggtttc gagcagccta
     5101 tgatttctta tggttacgct gtgaaagtgg tgagattgat aaagaaatcg ccgaatggtg
     5161 gcatgcattc caacacgcca gcccggccaa tagagagtcg atgcttatca aagaaacgcc
     5221 tgttaaaaaa cgcaaacgca acacccgaaa acgaaaaccc atcgtatctg cagaatctga
     5281 gaagaacgat cagtagatcc aattgcacat aacgatgaac aattcagagc ttctccc
//
LOCUS       DAOQKQ010000173         5223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_8524_length_5223_cov_5.666215, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000173 DAOQKQ010000000
VERSION     DAOQKQ010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5223
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8524_length_5223_cov_5.666215"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="PKZ49_10425"
     CDS             <1..888
                     /locus_tag="PKZ49_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HND37021.1"
                     /translation="VIWGSPIELGTQDNIRALRDSLFRSKLLPAFEMSDQKLQVFLDE
                     IRSFQPKILFGYPSALSFIARYAEKTGEQMNDLGIKVAFVTSERLYDEQRQQISKTFN
                     CPVANGYGGRDAGFIAHECPAGGMHITAEDVIVEILDNQGIPVPCGESGEIVITHLAS
                     SDFPFIRYRTGDIGKLDDQLCSCGRGLPLLKEIEGRSTDFVVAQDGTVMHGLALIYIL
                     RKIPEINAFKIIQENLDLTRILIVSKSALDAATIKLITNEFQTRLGNDITIKIEEVNE
                     IPLEKSGKFRYVISKVIPP"
     gene            888..1838
                     /locus_tag="PKZ49_10430"
     CDS             888..1838
                     /locus_tag="PKZ49_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HND37022.1"
                     /translation="MEQLLLEPTQDLSMRFNRIAVNTISEKNNNAQTVRYIAFTDWGN
                     SENTQVVICAHGLTRNGRDFDYLAKALQDQFRVICIDIVGRGQSDWLTNAEEYNQPAI
                     YLSDIKILLTHILAQYEQPVHLNWVGISMGGLIGMILASQDLPIPIKSIIMSDIGPRI
                     PIDAILRFKRYVGEDPKFTDIDQLENYIRVISAPFGELTKDQWRHLTLYSACKYSEGV
                     YGFRYDPRIAINFHESFEHDIDLWSFWDQLTIPALVLHGEKSDVLLPQIAEEMRIRGP
                     RAKIIKISNVGHAPLLVNEEQISHVKGFLLKQIQLHKNTK"
     gene            2026..2310
                     /locus_tag="PKZ49_10435"
     CDS             2026..2310
                     /locus_tag="PKZ49_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HND37023.1"
                     /translation="MKLFKKIPNPREVRQKLNMNQQEFWSKVGVTQSGGSRYESGREM
                     PKAVRELVRLVHIDRIDLLKIKRDDILIAAMLKTQHPELYKDLKKSIKMR"
     gene            complement(2379..2834)
                     /locus_tag="PKZ49_10440"
     CDS             complement(2379..2834)
                     /locus_tag="PKZ49_10440"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HND37024.1"
                     /translation="MNYLVFVILVTLLSFSALSFASNESDKENVLGLWLTKKKDVVVQ
                     IKYCEAKTLCGYVSWISPEEMNEKNATLCGRKVLWDARLADDQPIRWVGGVLYKADED
                     KYYSANLQLIDADTLALRAYIGVPLLGKTKKLTRTSEKVHPSCIVPNQP"
     gene            2987..4135
                     /locus_tag="PKZ49_10445"
     CDS             2987..4135
                     /locus_tag="PKZ49_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HND37025.1"
                     /translation="MLSNLTPPRRPDPFARRMIIIALTIAALMLLWRTLPLIESLFVP
                     EKGEIRTVTARGDLALDERTTIELFEQSRDSVVFISTTKLVRDIWTRNVFAVPRGNGS
                     GFFWDDAGHIVTNFHVIQGASEATVKLADGRDYKAALIGVSPSHDIAVLKIGVGFKRP
                     NPIPIGTSHNLKVGQKVFAIGNPFGLDWTLTTGIISALDRSLASDNGTAIEHLIQTDA
                     AINPGNSGGPLLDSAGRLIGINTAIYSPSGASAGIGFAVPVDTIMRVVPQIIDKGKYI
                     RPSLGISVDERFNDRIVQLLNIRGVVVLNVAPGSAAEKAGLKGAAISIEGNITPQDII
                     IAINGKKVESVSKLLARIDDFNVGDVVTLTVFRNEQTREVHITLQPGV"
     gene            4168..4689
                     /locus_tag="PKZ49_10450"
     CDS             4168..4689
                     /locus_tag="PKZ49_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HND37026.1"
                     /translation="MDFSDFKLDSASVDQSQAICELLNSAYRGTDGWTTEVDIISGKR
                     ADRNEIEAAIKLPNSYFFVANQSNQLASCIHITKKDDHAQFGFFAVQPSLQGNGLGTY
                     VLKQAEIFAKTRLYSKKLLMIVVSQRTELIAFYERRGYRRTGQVENLPANIGIPKRIN
                     LTIDYLEKSIATT"
     gene            4834..5070
                     /gene="rpmB"
                     /locus_tag="PKZ49_10455"
     CDS             4834..5070
                     /gene="rpmB"
                     /locus_tag="PKZ49_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HND37027.1"
                     /translation="MARVCEVTGKKPMTGHHVSHANNKTKRRFLPNLQRRRFWVESEN
                     RWVTLRLTNAGLRTIDKNGIDAVLAKIRARGVDV"
     gene            5120..>5223
                     /gene="rpmG"
                     /locus_tag="PKZ49_10460"
     CDS             5120..>5223
                     /gene="rpmG"
                     /locus_tag="PKZ49_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HND37028.1"
                     /translation="MREKIKLESSAGTGHFYTTTKNKKTKPEKLEMKK"
BASE COUNT         1681 a         1007 c         1002 g         1533 t
ORIGIN      
        1 gtaatttggg gatctcctat cgaattagga acccaggata atatccgcgc gttacgagac
       61 tcattatttc ggagcaaatt actgcctgct tttgaaatgt cagatcagaa attacaagtt
      121 ttccttgatg aaattcgttc ttttcagcct aaaatactct ttggctatcc ttcagcccta
      181 tcgtttatcg cgcgttatgc tgaaaaaacc ggcgaacaaa tgaatgatct cggaataaaa
      241 gttgcatttg taacttctga gcgtttgtac gatgaacagc gccagcagat cagcaaaacg
      301 tttaattgcc ctgttgccaa tggctatggg gggcgcgatg ctggatttat tgcgcatgaa
      361 tgcccagcag gtggtatgca tattactgct gaggatgtca ttgttgaaat ccttgataac
      421 caaggaatac ctgtaccgtg tggtgaatcc ggtgaaatcg tcattacaca tcttgcaagt
      481 agcgactttc cattcatacg ctaccgaacc ggcgatattg gcaagctcga tgatcagtta
      541 tgtagctgcg ggagaggtct accactatta aaagaaatcg aggggcgaag cactgatttt
      601 gttgttgctc aagatggtac cgtgatgcat ggacttgctt tgatttatat attaagaaag
      661 attcctgaaa ttaatgcatt caagataata caagaaaacc tcgatttaac acgtatatta
      721 attgtgagta aatctgcact agatgcggca acaataaagt taattaccaa tgaatttcaa
      781 acaagattag gcaacgatat tacgattaaa atagaagagg ttaatgaaat tcctttagag
      841 aaatctggga aatttcgcta cgtcatcagt aaagtaatac caccttaatg gagcagttat
      901 tactagaacc cactcaagat ttatctatgc gctttaatcg aattgctgtt aatacgattt
      961 ctgaaaaaaa taataatgct cagacagttc gctatatcgc gtttactgat tggggaaatt
     1021 cggaaaatac acaggttgtt atctgcgctc acggacttac tcgaaatggt cgggattttg
     1081 attatcttgc aaaagcttta caagaccagt ttagagttat ctgcattgac atcgtcggtc
     1141 gcggacaaag tgattggctt acgaatgcag aagaatacaa tcaaccagcg atctacctat
     1201 ctgatataaa aatcttacta acccatatcc tagctcagta tgagcaaccc gttcatctaa
     1261 actgggtagg catatcaatg ggggggctta ttggtatgat acttgccagc caggatttac
     1321 caattcccat aaaatcgatt ataatgagcg acatagggcc tcgaattccc attgatgcca
     1381 ttctccgctt taagcgctat gttggtgaag atcctaaatt cactgatatc gatcagctag
     1441 aaaactatat ccgagttatt tcagcgccat ttggagagtt aactaaagat cagtggcgcc
     1501 atctgacact gtatagtgcc tgcaaatatt ctgaaggcgt gtatggattt cgttacgatc
     1561 cccgtattgc tatcaatttt catgaaagct ttgagcatga tattgattta tggtcattct
     1621 gggatcagtt aactattcct gccttggtac tgcacggtga aaaatcagat gttttacttc
     1681 cccaaattgc tgaagaaatg agaattcgtg gcccaagagc aaaaataatt aaaatatcaa
     1741 atgtgggaca tgccccctta ttagtcaacg aggaacagat ctcacacgtt aaaggatttt
     1801 tattaaaaca aattcaattg cataaaaaca ctaaatagat ttaaagaaat aacgcataaa
     1861 ttccttttaa ataaagctta aattccatat agatactccg ataaaaatca aactaattct
     1921 gaatgaatat caccctgagt tagtattttt ttgatctatc tgaaattcga acaaaaaaat
     1981 tatttctaag tctaatcgct ttgatgacaa ttgagagaaa acattatgaa attattcaaa
     2041 aaaattccaa acccaagaga agtaaggcag aaactaaata tgaatcagca agaattctgg
     2101 agtaaagttg gcgttactca gagtggtggt tctcgttatg aaagtggcag agaaatgcca
     2161 aaagccgtca gagaacttgt aagactcgtg catatcgatc gtattgattt actcaaaatt
     2221 aaaagagatg acatactgat cgctgcaatg cttaaaacac aacatccaga gttatataaa
     2281 gatctgaaaa aatctatcaa aatgcggtag tttcttcatg aatcaacaag gtctgccttt
     2341 gttttaatgt caaagcagca tgacgcttac tacctgtttt atggttgatt aggaacgata
     2401 cacgaagggt gtactttctc tgaagtacgg gtaagttttt tggttttccc caataaaggc
     2461 accccaatat aagcacgaag tgctaatgta tcagcatcaa ttaattgcaa gttagcactg
     2521 tagtatttat cctcatctgc tttatacaaa acaccaccaa cccaccttat cggctgatca
     2581 tctgctaagc gtgcatccca caacaccttt cttccacata acgtagcatt cttctcgttc
     2641 atctcttccg gactaatcca agacacatac ccgcataaag tctttgcttc acagtatttg
     2701 atttgtacca caacatcttt cttcttcgtt aaccataatc cgagaacatt ttctttatct
     2761 gactcattag atgcgaaaga taaagcagaa aaagaaagca gcgtcaccaa tattacaaaa
     2821 accaaataat tcataaattc tctaaaatag cgttttccta ccataaatca cttatttaaa
     2881 taatcgtatt ctaaagtgat gtacattcta tcttgtttta gatcataagg tttattatta
     2941 gcattccata accaattttt gtatctactt acttttttta gtcaacatgc tttccaatct
     3001 aacgccacca agacgaccag atccgtttgc tcgccgcatg ataatcatcg cactaacgat
     3061 cgcagcgcta atgctacttt ggcgcacttt accactcatt gagtccctct tcgttccaga
     3121 aaaaggagaa attagaacag taactgcaag aggagatctt gctctagatg aacgcactac
     3181 aattgaatta tttgagcaat cccgtgattc cgttgtattc atctccacaa ccaaattagt
     3241 acgagatatc tggactcgca atgtatttgc tgttccaaga ggcaatggat ctggtttctt
     3301 ctgggacgat gcaggtcata ttgtcacaaa tttccatgtg atccaaggtg cgagtgaagc
     3361 aacagtcaaa ttagcggatg ggcgtgatta taaagcggcg ctcattggag taagcccttc
     3421 acatgatatt gctgtactaa aaattggtgt gggcttcaag cgtcctaacc ctattccaat
     3481 aggaaccagc cataatctca aagttgggca aaaagttttt gcaatcggca atccctttgg
     3541 actcgattgg actttaacga caggtatcat ttcagcgcta gatcgttccc tagcaagtga
     3601 taatggaacg gcaatcgaac acttaatcca gaccgacgct gcgattaatc cgggtaactc
     3661 gggaggccct cttctcgact cagcgggtag attaatcggt atcaatactg cgatttatag
     3721 tcccagtggc gcatcggccg ggatcggatt tgctgtaccc gtagatacga ttatgcgagt
     3781 tgttcctcag atcattgata aagggaaata tattcggccc tcgcttggta tcagtgttga
     3841 tgaacgtttt aatgatcgaa ttgtacaatt actcaatatc cgtggtgtag tcgtattaaa
     3901 tgtagcgcct ggatccgctg ctgaaaaagc tgggttaaaa ggcgcggcta ttagcataga
     3961 aggtaatatt accccgcaag atatcatcat cgctattaat gggaagaaag tagaatctgt
     4021 ctcaaaatta cttgccagaa ttgatgactt taacgttgga gatgtcgtaa cccttacggt
     4081 attccgaaat gaacaaacgc gagaagtcca tattacgctg caacctggtg tttaaataat
     4141 attcgcataa tacaattgcg gtcgattatg gatttttctg attttaagct cgatagcgct
     4201 agcgtagatc aatctcaggc aatttgtgaa ttattgaatt ctgcttaccg tggaaccgat
     4261 ggttggacta ctgaagtaga tattatttcg ggcaagcgag ctgatcgtaa tgagattgaa
     4321 gcagcaatta agctacctaa tagctatttt tttgtagcta accaatcaaa ccaactcgca
     4381 tcgtgtatcc atatcactaa aaaggatgat catgcccaat ttggtttttt tgctgttcaa
     4441 ccatctctgc aaggtaatgg attaggcaca tatgtactca aacaagcaga aatcttcgca
     4501 aaaactcgct tatattcaaa aaaactactc atgattgtcg taagtcagcg cactgaattg
     4561 attgcttttt atgagcgaag gggatatcgg cgaacaggcc aagtcgaaaa tttaccagca
     4621 aatatcggca ttcctaaacg aataaattta accattgatt atttagaaaa gagtatcgct
     4681 acgacttgat taatttaaaa aataatctga tcaggattta atatcaaccg tctcagttaa
     4741 ttattctcac tacactatga taattaaacc gattgtgtga atcttttgat ttaggataca
     4801 ataaaaaatt atagaaaaat ttgaggattt aaaatggctc gtgtatgtga agttacaggt
     4861 aagaaaccaa tgactggtca tcatgtatct catgcaaaca ataaaacgaa aaggcgattc
     4921 ttgcctaatt tacaacgacg tcgcttctgg gttgaaagcg aaaatcgctg ggttacacta
     4981 cgcctgacca acgcgggatt acggactatc gataaaaatg gtatcgatgc tgtacttgca
     5041 aaaataaggg ctcgtggggt tgatgtctaa ttacggttaa aattagcaag ctatttcatt
     5101 aatttctgga aaatattata tgcgtgaaaa aattaaatta gaatcttctg ctggaacagg
     5161 acatttctat acaacaacaa aaaataagaa aacgaaaccc gagaaattag aaatgaaaaa
     5221 att
//
LOCUS       DAOQKQ010000174         5220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_8535_length_5220_cov_6.091384, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000174 DAOQKQ010000000
VERSION     DAOQKQ010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8535_length_5220_cov_6.091384"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..639
                     /locus_tag="PKZ49_10465"
     CDS             94..639
                     /locus_tag="PKZ49_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HND37029.1"
                     /translation="MTTSFPEPYRSNFKLPPLKPDIAFVYSRLAHFLAFAAGAGLSKY
                     APGTIGTLVAFPIYWLLQLLFDPIALLLVIDIFFIIGIWACGITGKALHAPDHSGMVW
                     DEIVAFLLVLYFIPDHWLWQISAFILFRFFDIVKPPPIKYYEKKFHGGVGVMFDDLLA
                     AFFTLLCLAGWKSIVLFESYF"
     gene            649..1164
                     /locus_tag="PKZ49_10470"
     CDS             649..1164
                     /locus_tag="PKZ49_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HND37030.1"
                     /translation="MIVDDSVLSAYAQQVGVLLTSKHLRLVTAESCTGGWIGQSITAI
                     AGSSGWYECGFITYSNQAKHRMLGVQATTLLKYGAVSEQTAREMVTGALKMSTAQVAI
                     SVTGVAGPSGGSKEKPVGMVCFAWEFKQSAIHSTTHFFSGDRESVRRQAVAAALLGII
                     ELLEGISPSLV"
     gene            complement(1153..1767)
                     /locus_tag="PKZ49_10475"
     CDS             complement(1153..1767)
                     /locus_tag="PKZ49_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HND37031.1"
                     /translation="MEINQKTDNKRISAVARFLFLSRWLQLPLYLGLVLAQCVYVFHF
                     WVELTDLVGAIMGNKESLKHILHIVTPEEAEAPLKITETAIMLVVLGLIDVVMISNLL
                     IMVIIGGYETFVSRMNLEGHPDQPEWLSHVNASVLKIKLATAIIGISSIHLLKTFINA
                     DAYDEKTLIAQTSIHITFLISAVAIAYTDRLMSQTLSHGEASNK"
     gene            complement(1889..2776)
                     /gene="sucD"
                     /locus_tag="PKZ49_10480"
     CDS             complement(1889..2776)
                     /gene="sucD"
                     /locus_tag="PKZ49_10480"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019867325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HND37032.1"
                     /translation="MAILINENTRIIVQGFTGRIGTFHAQEMIDYGSKVVGGVTPGKG
                     GQTHLGLPVFNTVKEAVEKVGAEASIVFVPPAFAADSIMEAADAGIKYCVSITDGIPT
                     QDMMTVKSFLRRFPKERRMMMTGPNCAGTISPGRAMLGIMPGHIYMRGNVGIVGRSGT
                     LGYEAADQMRRLGIGITTSVGIGGDPIIGSSHRDVLEKLEQDSETKVALMIGEIGGPQ
                     EVEAGIFAKENMTKPLVAYIAGLTAPTGRRMGHAGAIISSAGESAAEKVERLKELGVT
                     ICPTPSLMGQTVAEVLAKL"
     gene            complement(2789..3970)
                     /locus_tag="PKZ49_10485"
     CDS             complement(2789..3970)
                     /locus_tag="PKZ49_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate--CoA ligase subunit beta"
                     /protein_id="HND37033.1"
                     /translation="MDIHEYQAKEILAQYGVKIAEGGLAHTVEEAVQRSREIDGNVWV
                     VKAQIHSGARGKAGGIKVCKTHPEVEAAAGALLGKKLVTHQTGAAGKLCSKLYIEAGT
                     DIARELYLSFLIDRSHERIIMVGSAQGGMEIERLAVTDPDAIIKIHIEPAVGLQDFQA
                     RTMAFALGLGEDLLNHAVKTIKGCYRAMRDLDANILEINPLVVTKSNELMALDAKMSF
                     DENALFRRQKIAELRDKTQIDPREVAAAEAGLSYVGLDGDIGCMINGAGLAMATMDMI
                     KLAGGEPANFLDVGGGASAERTEKAFRLVLADQGVKAMLVNIFAGINRCDWIAEGVVQ
                     AVKNIDMKIPLVVRLSGTNVEEGCRIIDESGLPIITAETLADAAEKVVKARNEVVAKQ
                     G"
     gene            complement(4019..4969)
                     /locus_tag="PKZ49_10490"
     CDS             complement(4019..4969)
                     /locus_tag="PKZ49_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HND37034.1"
                     /translation="MSHTLYETKIQRVQRCELAVPGSRPEMFEKALKSGVDFIFLDLE
                     DAVAPDDKIQARKNIIQAINDLDWKGHGITLSVRINGLDTQYMVRDVVDLVEQAGHKI
                     DTILIPKVGVYGDVYMVEAMLNQLEMQQRLNNRIGIEALIETALGMANVEDIARNGAS
                     GRLEALHFGVADYAASNRARTTNIGGLNPDYPGDQWHAAISRMTVACRAYGLRPIDGP
                     FGDIQDPDGYKQAARRAAALGCEGKWAIHPTQIALANEVFTPLSAEVEKAKRILAALK
                     EAAAQGKGAASLDGRLIDAASERMANNIVKAAEAIAAKNK"
BASE COUNT         1395 a         1296 c         1068 g         1461 t
ORIGIN      
        1 ccccactgac gtgtatcgga aaaatattac aaggagaagg tttatctata gtaaactcaa
       61 ctggaacagt aattgagcat acgaaaaaag gctatgacca cttcctttcc tgaaccgtat
      121 cgatccaatt tcaagcttcc tccccttaag ccggatatag cctttgtata tagccgctta
      181 gcacactttt tagcctttgc tgccggtgca ggactcagta aatacgcacc gggcacgatt
      241 ggaacccttg ttgctttccc tatctattgg ctgctgcagt tgttatttga tccaatcgca
      301 cttttgctcg tcatcgatat ctttttcatt attggcattt gggcgtgcgg catcaccggg
      361 aaagcgctgc atgcacccga tcattcaggt atggtgtggg acgaaatcgt tgcgttcttg
      421 cttgttttat atttcatacc agaccattgg ttgtggcaaa tcagtgcgtt tatcttattt
      481 cggttttttg atatcgtgaa gccgccgccc attaaatact acgaaaaaaa attccacggt
      541 ggggtaggcg taatgtttga tgacttactc gcggcttttt ttaccctcct gtgtctagcc
      601 ggatggaaat cgatcgtctt atttgaaagt tatttttagc gcactgcaat gatcgtcgat
      661 gattcagtac tttctgctta tgcgcaacaa gttggtgttt tgctcaccag caagcatcta
      721 aggttggtta ctgctgaatc atgcaccggg ggttggatag ggcaatccat tacggctatt
      781 gcagggagtt caggctggta tgaatgtggc tttatcacgt atagtaatca agcaaagcac
      841 cggatgttgg gcgttcaagc gacaacatta cttaaatatg gcgctgtttc agaacaaact
      901 gcgcgagaaa tggtaacagg tgctcttaaa atgagcaccg cacaagtagc gatatcagta
      961 actggtgttg ccggtccgag tggtggttcg aaagaaaaac ccgtaggaat ggtatgtttc
     1021 gcctgggaat ttaaacaaag cgcaattcat agcacgacac attttttttc aggtgaccgt
     1081 gagtcagtca ggcggcaggc cgtggctgcc gccttgcttg gtatcatcga gttactcgaa
     1141 ggaatttcac cttcacttgt ttgaggcttc gccatgactc aacgtttgtg acatgaggcg
     1201 atccgtataa gcaatcgcta cagcagagat cagaaaagtg atatggatcg atgtctgagc
     1261 aatcaatgtt ttctcatcat aagcatctgc attgatgaat gttttcagta gatgaatcga
     1321 tgaaatacca atgatcgcgg ttgcaagctt gatcttcaga accgatgcgt taacatgcga
     1381 caaccattcc ggttgatctg ggtgcccctc aagattcatt cgtgatacaa aagtttcata
     1441 gccaccgatg atcaccatga tcagcaaatt ggaaatcatt acgacatcaa ttaaacccag
     1501 cacgaccagc ataattgcag tttcagtaat ttttagcggt gcttctgcct cttcaggtgt
     1561 tacgatatgt aaaatatgtt tgagtgattc tttgtttccc ataatggcac caaccagatc
     1621 cgttagctca acccaaaaat gaaaaacata aacacattgc gccagcacaa gacccagata
     1681 aagcggtagt tgtagccagc gtgacagaaa aagaaaacgt gcaaccgcag aaattcgttt
     1741 gttatcggtt ttctgattta tttccataac tctcaagttc agtttttgaa ttaacaaaga
     1801 taacccgtaa agtatgaacc ttcacaggat aattcaagcg atcaggccaa gccgcttgta
     1861 cttggcctaa ttgtataaag acacgaaatt atagttttgc taaaacttca gcaacggttt
     1921 gccccatcaa agaaggggtt ggacaaatcg tgacgcctaa ttcttttagt cgttcgactt
     1981 tctcagccgc actctcacca gcagaagaga taattgcgcc agcatgaccc atacggcggc
     2041 cagtaggtgc agtaagtcct gcaatgtaag ccaccaaagg cttcgtcata ttctctttag
     2101 caaagatacc cgcttcaact tcttgcggtc caccaatttc accgatcatg agcgccactt
     2161 tggtttcaga gtcttgctcc agtttctcca gtacatcgcg atgggaacta ccaatgatcg
     2221 gatctccacc aatgccaaca gacgttgtaa taccgattcc taatcggcgc atttggtcag
     2281 cagcctcata gcccaatgtt ccggaacgac caacaatgcc aacattaccg cgcatataga
     2341 tatggccggg catgatgccg agcatggcac gtccagggct aatcgttcct gcacaattgg
     2401 ggcctgtcat catcatacgt cgttcttttg gaaagcggcg tagaaaactt ttgacggtca
     2461 tcatgtcttg agtcggaata ccatcggtaa tggagacaca gtatttgatt ccagcatcag
     2521 ccgcttccat aattgaatcg gctgcaaatg cgggtggtac aaaaacaatg ctagcttccg
     2581 cgccaacttt ttccacggct tcttttaccg tgttaaatac cggtaagcct aaatgcgttt
     2641 gccccccttt accgggcgtt actccgccaa caaccttaga accataatcg atcatctcct
     2701 gtgcgtggaa agtgccgatc ctaccagtaa atccctgaac gatgattcgt gtattttcat
     2761 ttattaaaat agccacttga tgttcccctt aaccttgttt tgcaaccact tcgttgcgcg
     2821 ctttaaccac tttttctgca gcatcagcca aagtttccgc cgtaatgatc ggcaagccgc
     2881 tttcatcaat gatgcgacaa ccttcttcga cattagtacc ggataaacgg acaacgagtg
     2941 gaattttcat atcgatgttc ttaactgctt gcacaacccc ttccgcaatc cagtcgcaac
     3001 gattaatacc tgcaaaaata ttaaccagca tggctttcac accttggtcc gccagaacga
     3061 gacgaaatgc tttttcagtt cgttccgcag aggcgccgcc acccacatca agaaaattag
     3121 ccggctcgcc acctgccagt ttgatcatat ccatggtagc catggctaaa cctgcgccgt
     3181 tgatcatgca accgatatca ccatctaacc caacatagct caggcccgct tcagcggctg
     3241 caacttcgcg cggatcaatt tgggttttgt cacgtagctc agcaattttt tggcggcgaa
     3301 atagtgcatt ttcgtcaaaa ctcattttgg catccagcgc catgagttca ttgcttttgg
     3361 tcaccactaa tggattgatt tccagaatat tggcatccaa atctcgcata gcacgataac
     3421 accctttgat ggtttttacc gcatgattga gcagatcttc acccaacccc aatgcaaagg
     3481 ccatggtacg tgcttggaaa tcttgcaggc ccactgccgg ctcaatatgg attttaatga
     3541 tggcatccgg atccgttact gctaaccgtt cgatctccat tccaccttgt gcagagccaa
     3601 ccatgatgat gcgttcatga ctgcgatcaa tcaaaaaaga aagataaagt tctcttgcaa
     3661 tatcagttcc cgcttcgata taaagtttag aacaaagctt gccagcagca cccgtttgat
     3721 gtgtgaccaa tttctttcca agcaaggccc ctgcggcagc ctcaacctca gggtgtgttt
     3781 tacaaacttt aataccaccc gctttgccac gtgcgccgga atgaatttgt gctttgacaa
     3841 cccatacatt accatcgatt tcgcgactac gttgcacggc ttcttcaacg gtatgtgcaa
     3901 gtcctccttc cgcaatctta acgccatatt gagccaagat ttctttggct tggtactcat
     3961 gaatatccaa tgcctttccc cctattaatt caatcaatca tataaaactt agtaatggct
     4021 atttattttt ggcagcaatg gcttctgctg ctttgacgat attattagcc atccgctcag
     4081 atgccgcatc aatcaatcga ccatccaacg acgcagcgcc tttaccctgt gcagccgctt
     4141 ctttcaatgc cgctaaaatc cgcttggctt tttcaacttc agcagatagc ggtgtaaata
     4201 cttcatttgc taatgcgatc tgagtcgggt gaatcgccca tttaccttca catcctaagg
     4261 ctgctgctct tcgcgccgct tgtttatagc catctggatc ttgaatatct ccaaaaggcc
     4321 catcaatcgg tcgcaaacca taagcacggc acgctaccgt catgcggcta attgctgcat
     4381 gccattgatc gccaggataa tcaggattga gtccaccaat attggtcgtt ctagcgcgat
     4441 tacttgccgc ataatcagcc acaccaaaat gcagcgcttc caaacgacca gaagcaccat
     4501 tgcgcgcaat atcttcaaca ttcgccatgc ccagcgctgt ttcaatcaaa gcttcaatgc
     4561 cgattctatt gtttaagcgc tgctgcattt ccagttgatt gagcatcgct tccaccatat
     4621 acacatcacc atagacaccg actttgggaa tcaaaatagt gtcgattttg tgcccagctt
     4681 gttcgactaa atccacaaca tcacgcacca tgtattgtgt atccaaacca ttgatgcgca
     4741 ctgatagcgt gataccatgg cctttccaat caagatcatt gatagcctga ataatatttt
     4801 ttcttgcttg aattttgtca tctggcgcaa cggcatcttc gagatccaag aaaataaaat
     4861 cgaccccact tttcagtgcc ttctcgaaca tttcaggacg tgaacccggc acagctagct
     4921 cacaacgctg cacgcgctga attttagttt catacaaggt gtgactcatt tttcctccag
     4981 taaaaaaatc ttgttttaat gcgccgaact acgatgaaaa aaaggaaaca aaatcaactc
     5041 aaagctggat taaccgtcaa gctggcttca ataggtgggc gttatgtggt ccagaatttc
     5101 aaaataattg ggcactattt atcatttaaa tgcagcatta taccacttgc acccgtggaa
     5161 ttttggtgaa ggtaatcatt gcacggtcaa ggatcgtgat aaatttcaca gccagtaacc
//
LOCUS       DAOQKQ010000175         5180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_8652_length_5180_cov_5.272585, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000175 DAOQKQ010000000
VERSION     DAOQKQ010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8652_length_5180_cov_5.272585"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /gene="metW"
                     /locus_tag="PKZ49_10495"
     CDS             <1..509
                     /gene="metW"
                     /locus_tag="PKZ49_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HND37035.1"
                     /translation="GALLRYLTDTRQIKGYGVEIDDTNILACLNNRINVIQSDLESGL
                     SGFESNSFDYVILSQTLQAMKHTENIIREMLRVGKEGIVTFPNFGHWKNRLQILRGFM
                     PVSPTLPYEWFDTPNIHLCTLKDFEQFCHQHGVHILEKRVMSDNQQTTFAPNLFGMLA
                     FYRFTQRK"
     gene            complement(648..3410)
                     /locus_tag="PKZ49_10500"
     CDS             complement(648..3410)
                     /locus_tag="PKZ49_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HND37036.1"
                     /translation="MSTSIYGKRMRKISITFILMALWLLSSVAWAATHTISISAVQLP
                     NGQYGYKLNNHTSSDGAVPNYPAEAVIPGPTLFVKQGDQVTVQLRNETNIKIGFNVPG
                     LTNGNVAPANPGQTKSYTLNTQQTGTYPYFDEKSQLLGLFGAIVVDDASGQVQSFVDG
                     DGKITAVNRSQLDKEFVMFMVGSTFWGAEISRDGTQKPLWTNPTLGAVMNNLVRFHVL
                     SVGMAHTFHLHAHRWINPAANPNTATAEDIIDVHLMKNPHDSHVFTIKAGTGVGPGVW
                     QYHCHLVSHMESGMNGKFLVVAADSGDPGNSIAGASPSGAIFLNPSDDPGLVTFIVSD
                     EPGSWFRSARGDALSPVTFTKSLEVIPPGSSVHFVMSDTHGVHTITSLLWPSEAGNNN
                     HPGGDHFMIPFDQTKAYRGGGIVQLNVPGLYVFTCKVHPYMFGAVIVDSPGTTGLDLG
                     NAIDLVSGIKNLPTSSDLATRLLRTFFVATTQGNWQDYTAPTPWHVNYPNVPVETDLG
                     TLNLKDVLEARYGQDITLPAVTAPTIPGVGEVWINTQFEKTAGKTKPGTITVLDTATW
                     TLKRKIALPEINMNNPHNVWPNRDHTIMYQTQWFDTKLTLIDQKTGKLIKNLQVGDTP
                     SHVVTLPNSDDVTVAINGENGVVIIPAGTTEVNYMMPTQHHGQVPANPHGHWVSADGS
                     RIITPNINTSDTGIYNADTGQIVSRTQTGNGAPGPHPIAIGMMPDSSKIYASNLLHHS
                     LSVLDGNNGALLKTINLIENYNPITGDITGPVGVLPIQTPVSPDGKAVVLAATGGQIL
                     VIDTKTDSIVKMFACDAGCHGANFGAKQGGGYYAYVTSKFSNRLIVVDIDPNNDGDIS
                     DADIVGSISMVAGNNVAKDDTISSLAGYGGQGIVALPNVYNGWVQNLTTEWASGLTSS
                     QRNPVQ"
     gene            complement(3430..4455)
                     /locus_tag="PKZ49_10505"
     CDS             complement(3430..4455)
                     /locus_tag="PKZ49_10505"
                     /inference="COORDINATES: protein motif:HMM:NF019352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HND37037.1"
                     /translation="MKDKLEYEFQSRSCHALPKPKELANPKLASWLVLASMVLGLGGV
                     TSVLAGNTHQIKMVAEEVDPTGNLGLYAYRMMQHQVVDGNGNTVDLSGNYSAVATIPG
                     PTVIMSEGDVAEIELMHQFDPDNPKQEHVSLHVHGVHYDKESDGTLKYINLYKDESAT
                     PVLSYNYRWVAAPGTAGTWAYHDHNMNSHNGAEDRGLFGALIVNPASGFLPQDINGTG
                     SGNTPLSRIQKEFVLYIGDDAFWGMEINNANGAQTALGVNPNLNAQRNSDIRIHLIAL
                     GTNFHQFELPSYTWVDPGTRRRISAKAIGPLEKHVFAISASHSASYLDTAFSSKLLGM
                     KGQLIVD"
BASE COUNT         1449 a         1200 c         1041 g         1490 t
ORIGIN      
        1 atggtgcgct attacgctat cttactgata cacgccagat caaaggatac ggtgtggaaa
       61 ttgatgatac gaatattctg gcctgcctga ataaccggat taacgtcatt caaagtgacc
      121 tagaatcggg cttatctggc tttgaatcca attcattcga ttacgttatt ctgtcccaaa
      181 cgctgcaagc aatgaagcat accgagaata tcattcgtga gatgctccgt gtgggaaaag
      241 aaggcatcgt aacattccct aatttcggtc attggaagaa tcgtttacag atcctgcggg
      301 gctttatgcc ggtttcaccg acattaccct atgaatggtt tgatacaccg aatattcatc
      361 tttgcacact caaagacttc gagcaattct gccatcaaca tggggttcac attttagaaa
      421 aacgagtcat gtcagataat cagcaaacca catttgcacc caacttgttt ggaatgcttg
      481 ccttttatcg gtttacacaa agaaagtaga aagctctcct ctctctaccc actatctgaa
      541 aaagcttaaa ccttcattct ttaactgctc acaaaacaaa aaaaccgatg accaaactat
      601 gaatctggtc atcggctatt gcgaatcaac taatcgcgag ttgctgctta ttgaaccggg
      661 ttacgttgac tgctcgtcaa acctgaagcc cattcagtcg ttaggttttg tacccagcca
      721 ttgtacacgt taggtagcgc tacaatccct tggccaccat aacctgccaa actgctgatg
      781 gtgtcatcct tcgcgacatt gttgcctgcc accatggaga tggatccaac aatatccgca
      841 tccgatatat caccatcgtt atttgggtca atatccacta cgatcaggcg attagaaaat
      901 ttgctggtta cataagcata gtatccaccg ccttgtttgg cgccaaaatt ggcaccatga
      961 caaccagcat cgcacgcgaa cattttgacg atgctgtcgg ttttggtatc gataaccaaa
     1021 atttgaccgc ccgttgcagc cagaaccacc gctttaccgt ctggagaaac aggcgtttga
     1081 attggaagca caccgacagg gcctgtaata tcaccggtga tcggattgta attttcaatc
     1141 aaattaatgg tttttaataa tgccccatta tttccatcca agacgctcaa actatgatgg
     1201 agcaggtttg aagcgtatat tttgcttgaa tccggcatca taccgattgc aatgggatgc
     1261 ggcccaggtg caccattccc cgtttgcgtt cttgaaacga tttgcccggt atctgcatta
     1321 taaataccgg tatcgctggt atttatatta ggggtgatga tacgagaccc atcggctgat
     1381 acccaatgtc catggggatt tgcaggtact tgaccatgat gctgagtcgg catcatataa
     1441 ttgacttcgg tcgttcctgc cggaatgatg acaaccccat tttcaccatt gatcgcaacg
     1501 gttacatcat ccgaattcgg taatgtcacg acatgcgaag gcgtatctcc cacttgaagg
     1561 tttttaatca acttacctgt tttttgatcg atcaatgtca actttgtatc aaaccactga
     1621 gtttggtaca tgatcgtatg atcacgattt ggccaaacgt tgtggggatt attcatattg
     1681 atctcaggca atgcgatttt acgcttgagt gtccaagtag ctgtatccaa aaccgtaatg
     1741 gttcccggtt ttgtttttcc agctgttttc tcaaattgcg tgttaatcca tacctcaccc
     1801 actcctggga ttgttggagc agttacagcg ggcagggtaa tatcttgccc atatcttgct
     1861 tcaagcacat ctttcaaatt gagtgttccc aaatcagttt cgacaggtac attgggataa
     1921 ttgacatgcc aaggggttgg cgcggtatag tcttgccaat ttccttgcgt cgtggcaaca
     1981 aagaaggttc tcaataatcg ggtagccaaa tcactactcg tcggtaaatt tttgatgcct
     2041 gataccaaat cgatggcatt accgagatcc aaacccgttg ttcctgggct atccacgatg
     2101 actgcaccaa acatgtaagg atgaacctta caggtaaaaa catataaccc tggaacattc
     2161 aattgaacga tcccaccacc ccgatatgct ttggtttgat caaatggaat catgaaatga
     2221 tcgccacccg ggtgattatt gtttccggct tcgctgggcc acaataagct ggtgatggtg
     2281 tgaacaccgt gcgtatcgga cataacaaaa tgaacgctac ttcccggtgg aatgacttcc
     2341 agcgatttag tgaacgttac gggactcaat gcatcaccac gtgcacttct aaaccaactt
     2401 ccgggttcat cagaaacaat aaaggtaacg agaccaggat catcgctcgg gttcaagaaa
     2461 atagcgccac ttggtgaagc gcctgcaata ctgttacctg gatcgcctga gtctgcagcc
     2521 actaccaaga acttaccatt catacccgat tccatatgag aaaccaaatg gcagtggtat
     2581 tgccaaacac ctggccctac cccagttcct gctttaatgg taaatacgtg gctatcgtgc
     2641 ggatttttca tcaaatgaac gtcaatgata tcctcagcag tcgctgtatt tgggttagcc
     2701 gctggattga tccagcgatg ggcgtgtaaa tgaaacgtat gcgccattcc tacggaaagt
     2761 acatggaagc gcactaggtt attcatcact gcgcctaatg tcgggttcgt ccataatggt
     2821 ttttgtgtac catcacggct gatctcggct ccccagaacg tagaaccgac catgaacatg
     2881 acaaattcct tgtccagttg agagcgatta actgcagtaa ttttgccgtc accatcgacg
     2941 aaactttgca cttgaccact cgcatcatcg acgacaatcg caccaaataa gcctaacagt
     3001 tgcgatttct catcaaaata agggtaagtt cctgtttgtt gcgtattcag cgtatagctt
     3061 ttggtctgcc cgggatttgc aggcgcaaca ttgccattcg ttaatcctgg tacattgaac
     3121 ccaattttaa tattggtctc attacgcagt tgaaccgtta cttgatcccc ttgcttcacg
     3181 aataaagtag ggccaggaat cacggcttct gctgggtaat tgggtaccgc tccatcgcta
     3241 ctggtgtgat tattcaattt atagccatac tggccattcg gtaattgcac cgctgaaatt
     3301 gaaatcgtat gggttgctgc ccatgcaacc gatgatagca accatagtgc catcaatata
     3361 aacgttattg aaatctttct cattcttttt ccataaatag atgtactcat gaccttctcc
     3421 caataaatct tagtcaacaa tcaattgacc cttcattccc agtaatttgc tggagaaggc
     3481 ggtgtctaag taactcgctg aatgtgaagc actaattgca aacacatgct tttctagcgg
     3541 cccaatggct ttagcactaa ttcttctgcg agtgccggga tcaacccagg tatagctcgg
     3601 tagctcaaac tgatgaaagt tagtgcctaa cgcaatcaaa tgaatccgaa tatcagaatt
     3661 tctttgtgcg ttgagattgg gattaacgcc cagtgccgtt tgcgcaccat tggcattatt
     3721 aatttccatc ccccagaaag catcgtctcc gatgtacagt acaaattctt tctgaatccg
     3781 actcaacggg gtattgccgc tgccagtgcc attaatatcc tgtggcaaga atcctgaagc
     3841 agggttgacg atcaacgcac caaatagccc acgatcttca gcaccattat gactattcat
     3901 attgtgatca tgataagccc aagttcctgc agtgcctggc gcggctaccc agcggtagtt
     3961 ataagacaat accggtgttg cactttcatc tttatagagg ttgatatatt tgagtgtgcc
     4021 gtcactttct ttgtcgtagt gcacaccatg aacatgcaaa ctcacatgct cttgtttagg
     4081 attatccgga tcaaactgat gcatcaattc gatttctgcc acatcgcctt ccgacataat
     4141 gaccgtaggc ccagggatag tcgccacagc gctataattg ccggaaaggt caaccgtatt
     4201 accattacca tcgacaactt ggtgctgcat catccggtaa gcatataatc ctaaatttcc
     4261 ggtagggtct acttcttccg caaccatttt gatctgatgg gtgttgcctg ccagaactga
     4321 agtcactcct cccaatccta ataccatgga agccaaaaca agccaggatg ctaattttgg
     4381 gttggccaac tctttcggct taggtagtgc atgacatgag cgagactgaa actcatattc
     4441 aagtttgtct ttcattaaat tccctccatt ttgattcaac caaaaaaaag cctgtctgac
     4501 ccaattctaa tgggccatga caagctgctt tgctcctact tcaatttctg tgaatacact
     4561 cttattcttt ggaaaacact tctcaaaata atgtaaccat tcacaaaaat ttaataatta
     4621 ttattgttat gagagttaaa cgagcataaa ttatgccaat aataatttct attattattc
     4681 aataactagc gttgatattc ttatctgaaa tgattaagcg gatgtaatat cagctctttc
     4741 tgtaaaaaaa accgacgctt tgggggtcaa aaatgctcgt aatatgaaca tctagcattt
     4801 gtcaatatgt ccaggtgcga caaattgtcg cacctgagct taacaattaa ttaacaaaag
     4861 tgacatgtaa aattttctga caattgtggg tctgaatata gattaaattg acggttacag
     4921 ccgctaaaaa tcgccgcgca tttcttgaag atttaatcca ttgaagttaa gcagtatcaa
     4981 ttaatgcttt attttcaatt gactgatgaa aaatcttggg gtgttcagag aagtctatca
     5041 aaaatatcga catcactatg ctggctgata aagcctctta cgtaaaataa agcgatgttc
     5101 tctaccatat cccaatggag aacgattaac ttaacaacgc tagcaatttt taagttgtaa
     5161 aagcaatttt actttagacc
//
LOCUS       DAOQKQ010000176         5146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_8749_length_5146_cov_5.286584, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000176 DAOQKQ010000000
VERSION     DAOQKQ010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8749_length_5146_cov_5.286584"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PKZ49_10510"
     CDS             <1..483
                     /locus_tag="PKZ49_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HND37038.1"
                     /translation="KPPSIGLRGSHGCLRMYPEDITGIYDAVPVGTPVRVVNQPHLLG
                     WRGDHLYLQTYPILEDDKRNHKQRLRQSLANIRSTSKAKLGVRPQNKIDPVLLKEIIQ
                     SERTVSLPITQAPTPLLTLLDQHALKVQNTLPRYATWSGDSSQQLTVAEIVKMAEGEP
                     "
     gene            complement(505..1026)
                     /locus_tag="PKZ49_10515"
     CDS             complement(505..1026)
                     /locus_tag="PKZ49_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 domain-containing protein"
                     /protein_id="HND37039.1"
                     /translation="MRKVMLLIGCSVAALPSVFAESIIRPGLWEVTTKSDLLQLVPHV
                     PAEQMQQITRLAKQYGLEMPQISNGAANSKMCITPEMAKQEVPSYFFEGQTGCTVKNA
                     SRTGNRYQMELTCSNAQFKGEGYAEGVFMTPESFSGQAEFDSVIQGSPIVAHAETSGR
                     WVGEHCDVVTPIR"
     gene            1236..2591
                     /gene="msrA"
                     /locus_tag="PKZ49_10520"
     CDS             1236..2591
                     /gene="msrA"
                     /locus_tag="PKZ49_10520"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HND37040.1"
                     /translation="MKGKTMKTILTAAILFIMIITSLAIGKTITQNSGKSNQIMNSTT
                     DYIVFGMGCFWGAEKRMSEIPGVIDVESGYAGGDIVNANYQQILAHEKALRAGKAEAR
                     NHAEVIKVTFDPEKVSLETVLAKFWENHNPTQGDRQGNDIGSNYRSAIYYHNPAQLAQ
                     AQVTQQVYQHALTAAGYGAITTEILPLKNYLTAEEYHQNYLQKNPNGYCGLGGVGVKY
                     PQANQTLSQNPNATDAKIAELLKPLKGNELNHAQQLVIFEAENCEYCKLFDQDILNYW
                     QAEVPVVATLNSNPPEGWTLEKPLFAAPTIVLFRNGQEVARYTGYNGEITNFWKWLGF
                     QLLSPEQQKIAFQHGTEPPFTAPNLDEKRPGRFVDPITGATLFLSKTKFDSKTGWPSF
                     FDPVEGSVTLHNDDSLGMRRIEVRSASSGIHLGHVFNDGPAPSYKRYCINGNVLKFIP
                     D"
     gene            complement(2627..>5146)
                     /locus_tag="PKZ49_10525"
     CDS             complement(2627..>5146)
                     /locus_tag="PKZ49_10525"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HND37041.1"
                     /translation="SMEGKRNNRYDVTILINGLPLVQIELKRRGAELKVAFNQVNRYQ
                     HESFDAGAGLFQYVQLFIISNGVNTKYFANNKFQSFQQTFYWTDKDNNRLSELCEFAA
                     VFLKPCHIAKMITHYTVITEEGVLKVLRPYQFYATEALVDRVKHSNDNAYIWHTTGSG
                     KTLTSFKASQIIMRLPKVHKVLFVVDRKDLDYQTTREFNAFAKGSVDATANTHNLVKQ
                     LNDDSVKLIVTTLQKLNNAIIKEHYLDKIGHLKAKKFVFIFDECHRCQFGEIHQNIKR
                     FFSNAQLFGFTGTPIFAENANNGFGQLKTTRDLFGECLHKYVIVDAIRDENVLRFAVE
                     YVGRYRYKDSANELDIDVEAIDRQELLDSRQRLEKIVDYILAHHAQKTKTPGFTAMLC
                     VSSINTLIDYYELFKVKQAQAERPLKLATIFSYAANEENPQANGLIPEESPDVPSGAK
                     INQNSRDKLDEYINDYNALFGTKYSTHDNQSFYNYYQDIAKRVRSGEIDILLVVNMFL
                     TGFDSPRLNTLYVDKNLKYHGLVQAFSRTNRILNEKKSQGNIVCFRNLKAATDEAIAL
                     FSSKNAKETVLLEPYESYVQQFNQATEALLAIAPTVASVDTLPDEKAELAFVTQFREL
                     LRLRNILTTFADFNTDDLALAAQPFEDYKSKYLDIHDKVRKRQAVDKVSILDDVDFEL
                     SLIHRDEINVAYILNLLVSLNKLAPQEAKKRQKEIIDLVAGEVQLRSKRALIEAFMQE
                     NLPKLQPNDNVIQAFESYWADRKQQAFSELCSDENLIPPALEKLLSHYAFANRLPRDQ
                     EIVDALNFKPKILERKPVVQRVAEKIKSFIDTFIEGMGGSV"
BASE COUNT         1401 a         1323 c         1102 g         1320 t
ORIGIN      
        1 aaacctccta gtattggttt acgcggtagt catggatgcc ttcgtatgta tccggaggat
       61 ataaccggta tctatgatgc cgtaccagtt ggaacaccag ttcgcgtcgt caatcaacct
      121 catttgttag gatggcgcgg cgatcattta tatttgcaaa cctaccccat tctggaagac
      181 gacaaacgca atcacaagca gcgacttcgt cagtcattag cgaacatacg ttcaacctcc
      241 aaagctaagc ttggggtacg cccacaaaat aaaattgatc ccgtactcct taaagaaatc
      301 attcaaagtg agcgcaccgt ctctttgcca attacgcagg cacccactcc actactcacg
      361 ttactcgatc agcacgcgct caaggtccaa aatacgttac cgcgctacgc aacttggagt
      421 ggcgattcaa gccagcaact aacggttgcg gaaattgtaa aaatggccga aggcgaacct
      481 taatcgatcg ttggacatta tccattagcg tattggcgtg acaacatcac aatgctcacc
      541 gacccagcgc ccagacgtct cagcatgggc aacaatcggt gaaccttgaa tcacgctatc
      601 aaactcagct tgcccagaaa agctttctgg ggtcatgaac acaccttctg cataaccctc
      661 gcccttaaac tgggcattac tgcaagtaag ctccatctga taacgattcc cagtgcggga
      721 agcattttta acggtgcatc ctgtctggcc ctcaaagaaa taagaaggca cttcttgttt
      781 tgccatttcc ggcgtaatac acatttttga gttagcagca ccattactga tctggggcat
      841 ttctaggcca tactgcttgg ccaggcgggt aatttgctgc atttgctctg caggaacatg
      901 cggcaccaac tgaagtaaat cagatttcgt tgtaacctcc cacaatccag gtcgaatgat
      961 gctttcagca aaaactgagg gtaacgcagc aactgaacac ccaatcaata acataacctt
     1021 tcgcataaca tcctctttaa taaatatact gataaaaagc aatctacctg gctaatatta
     1081 gaaactacca tcttaacaga gctgctctaa tgctttcttt agaactcgcc attagtgtcc
     1141 agttctataa agctctaaat tttaaagccc gagaaacgat ggctcgctca cagaactaaa
     1201 aacagcacgt tatgtcgaaa tttaagtcat caagcatgaa aggtaaaaca atgaaaacaa
     1261 tccttacagc agccatatta ttcattatga taatcacctc cctcgccatt ggaaaaacca
     1321 ttacacaaaa ttccggaaaa tcgaatcaaa tcatgaattc aactacagat tatattgtct
     1381 tcggtatggg ttgcttctgg ggtgctgaga agcgcatgag cgaaattccc ggtgtcatcg
     1441 atgtggaaag cggctatgcc ggcggcgata tcgtcaatgc aaactatcag caaattctag
     1501 cccatgaaaa agcgttacgt gctggaaaag ctgaagctcg gaaccacgct gaagtcatca
     1561 aagtgacgtt cgatccagaa aaagtatcgc tggaaaccgt gctggcaaaa ttctgggaaa
     1621 atcataaccc cacccaaggc gatcgccagg gcaatgacat cggcagcaat taccgcagcg
     1681 cgatttacta ccacaaccct gctcagctag cccaggcaca agttactcag caagtctacc
     1741 agcacgcgct tactgccgca ggatatggcg ccatcaccac ggaaatcttg cctttaaaaa
     1801 attacctgac agcggaagaa tatcatcaga attatttgca aaagaatcct aatggctact
     1861 gtgggttggg cggtgtcggc gtaaagtacc cgcaagcaaa ccaaaccctg agccaaaatc
     1921 ctaacgcaac agatgccaaa attgctgaac tcctgaaacc gctcaaaggc aacgagttaa
     1981 accacgcgca acagctggtt attttcgaag cggagaactg tgaatactgc aagctgttcg
     2041 atcaagatat tcttaactac tggcaagcgg aagtccccgt cgtcgcaacc ctaaactcca
     2101 atcctcccga aggctggaca ttggaaaaac ccctgttcgc agcacctacc atcgttttat
     2161 ttcgcaatgg ccaagaagtc gcgcgctaca ccggctataa cggggaaatc accaatttct
     2221 ggaaatggct cggattccag ctattatccc ccgagcaaca aaaaattgcg ttccagcacg
     2281 gcacggaacc gccctttacc gcgcctaatc tcgatgaaaa aagaccaggt cgctttgtcg
     2341 atcccattac gggcgccact ttatttctga gtaaaacgaa atttgacagt aaaaccggtt
     2401 ggccaagttt cttcgacccc gtcgaaggat cggtaacact gcataacgac gattcgctcg
     2461 gtatgcgccg aatcgaagtc cgcagcgcaa gttccggcat ccatctcggt catgtgttca
     2521 acgatggccc tgcacccagc tataaacgct attgcattaa tggcaacgta ttgaaattca
     2581 taccggactg aaaatcagga gcgtgtttac agtcacgcca gcaacctcaa acactcccgc
     2641 ccatgccttc gataaaggta tcgataaacg atttgatttt ttcagccacc cgttgaacaa
     2701 ccggtttgcg ttccaggatt ttgggcttga aattcaacgc atcgacaatt tcctggtcac
     2761 gcggtaaacg gttggcaaac gcataatggc tgagcagttt ttccagcgct ggtggtatca
     2821 agttctcatc gctgcataat tcactaaaag cctgctgttt acgatccgcc caatagctct
     2881 caaacgcttg aatcacatta tcgttaggct gcagcttcgg cagattttct tgcatgaacg
     2941 cttctatcaa tgcgcgttta ctgcgcaatt gcacttcacc cgcaactaga tcgatgattt
     3001 ctttttgccg cttcttggct tcttgggggg ctaatttgtt gaggctcacc agcaaattca
     3061 gaatataagc cacattgatc tcatcgcgat gaatcaggct cagctcaaaa tccacatcat
     3121 cgaggatcga tactttgtcg accgcttgcc gcttcctgac tttatcgtgg atatccagat
     3181 atttgctctt atagtcttcg aaaggctgcg cggctagcgc caaatcgtcg gtattaaaat
     3241 ccgcaaaagt cgtcaaaatg ttacgcaagc gcagcaactc gcgaaactgc gtcacaaacg
     3301 ccaattccgc cttttcatcg ggtaaagtat ccacactggc aacggtcggc gcaatcgcca
     3361 gcaatgcttc cgttgcctga ttaaactgct gcacatagct ttcatagggt tccagtaata
     3421 ccgtttcctt ggcattttta ctagaaaata gcgcaatcgc ctcatcggtc gccgctttca
     3481 gattgcgaaa gcaaacgata ttgccctgcg atttcttttc attgagaata cggttggtgc
     3541 gcgaaaacgc ttgcaccaat ccatgatatt tgaggttttt atcgacatac agcgtattca
     3601 agcgcggact atcgaaaccg gtcaaaaaca tattcactac cagcaaaata tcgatttcac
     3661 cactgcgcac gcgcttggca atatcctggt aataattgta gaacgactgg ttatcgtgag
     3721 tgctgtattt cgtgccgaac agcgcattgt aatcgttgat atactcgtcc agcttgtcgc
     3781 gactgttctg gttgattttt gcaccgctcg gcacatcggg tgattcctcg ggaatcaagc
     3841 cgttagcttg cggattttct tcattcgcgg cataactgaa tatcgtcgcc agcttaagcg
     3901 gtcgttcagc ctgggcctgc ttcactttga acaattcata atagtcgatc agcgtattga
     3961 tactgctcac gcacaacatc gcggtaaagc ctggcgtttt ggttttctgc gcgtgatgcg
     4021 caaggatata atcgacgatc ttttccagcc gctgccggct atcgagcaat tcctggcggt
     4081 cgatggcctc cacgtcgata tccaactcat tggcgctatc cttataccgg tagcgaccca
     4141 catattccac cgcgaagcgc agtacgtttt catcgcgaat cgcatcgaca attacatatt
     4201 tgtgcaagca ttcgccgaac aaatcgcgcg tggtcttgag ttgcccgaaa ccattgttcg
     4261 cgttctccgc aaaaatcggg gtaccggtaa agccaaatag ctgcgcattg ctaaaaaagc
     4321 gtttgatatt ctgatgaatt tccccaaact ggcagcggtg gcattcatca aaaataaaca
     4381 cgaatttctt cgctttcaga tggccaatct tgtcgaggta atgttccttg ataatggcgt
     4441 tgttgagctt ctgcaacgtg gtaacgatca gtttcacgct atcatcgttc aattgcttca
     4501 ccaaattgtg cgtgttagcg gtagcatcca cactaccttt ggcaaacgca ttgaattcac
     4561 gcgtggtttg gtaatctaga tctttacgat cgaccacaaa taacacttta tgcactttcg
     4621 gcaagcgcat gatgatctgg ctggccttga atgaagtcaa agttttgccc gatcccgtgg
     4681 tgtgccagat ataagcattg tcgttgctat gtttgacccg gtctaccagc gcttcggttg
     4741 cgtaaaactg atacggccgc aaaaccttca acaccccttc ctcggtgatc acggtgtaat
     4801 gggtgatcat cttcgcgata tggcaaggtt tcaggaatac cgcggcaaat tcgcacaact
     4861 ccgataagcg gttattatcc ttatccgtcc agtaaaaggt ttgctgaaac gattgaaact
     4921 tgttgttggc aaaatacttg gtattgacgc cgttgctgat gatgaaaagc tgcacatact
     4981 gaaacaaccc agccccagcg tcgaacgatt catgttgata gcggttcacc tggttgaatg
     5041 ccactttcag ttccgcacca cgccgtttga gttcgatttg caccaggggt aatccgttga
     5101 tcaggatagt cacatcgtaa cggttgttgc gtttgccttc catgct
//
LOCUS       DAOQKQ010000177         5126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_8809_length_5126_cov_5.199369, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000177 DAOQKQ010000000
VERSION     DAOQKQ010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8809_length_5126_cov_5.199369"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PKZ49_10530"
     CDS             complement(<1..282)
                     /locus_tag="PKZ49_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HND37042.1"
                     /translation="MSARNSYSAGIYPITTPSGRVITGPPKGMYWRVSFEKFTELDKD
                     GRIWWGKKGDNVPRIKRFLSEVMEGIVPQSIWLHEEVGHIQEAKKEVMAV"
     gene            complement(355..516)
                     /locus_tag="PKZ49_10535"
                     /pseudo
     CDS             complement(355..516)
                     /locus_tag="PKZ49_10535"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(477..902)
                     /locus_tag="PKZ49_10540"
     CDS             complement(477..902)
                     /locus_tag="PKZ49_10540"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HND37043.1"
                     /translation="MKKQRLELTWIGKENRPKLEPRILLEDPARNYHAKHRVTDGDIF
                     DNRLIFGDNLLALKALEAEFAGKVKCVFIDPPYNTGSAFTHYDDGVEHSIWLSLMRDR
                     LEIIRRLLSEDVRCGSRLMTTRRITSRCCVMKCLGGRIL"
     gene            complement(916..2631)
                     /locus_tag="PKZ49_10545"
     CDS             complement(916..2631)
                     /locus_tag="PKZ49_10545"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing HNH endonuclease family
                     protein"
                     /protein_id="HND37044.1"
                     /translation="MSATNFKTENNTYRKLIGNGLAYQIPRFQRDYSWTDEEWEDLWS
                     DILGTIQEGGEPAHYMGYLVLQSQDEKNFDVIDGQQRLTTLSLIVLAVLKNLNRLIGE
                     GKNPDQNKQRLEQIRNTYIGYLDPVTLVTRSKLTLNRNNDSYFQTYLVPLGHLPVRGF
                     RASEHSLRKAFEWFEKRIWDYAKKHGGDEGVALASLVETMSDHLFFTVISVTDELNAY
                     KVFETLNARGVRLSSTDLLKNYLFSVLHKQNEHPNEMKALEDRWESMVSRLGSESFPD
                     FLRSHWNSRNTFVRQSDLFKTIRNKVGNRAAVFELLRGMEEDMDTYLALTNPEASHWD
                     STLKSYAQLLRMFRVRQPFPLILAAHRTLKNGQFTAVLRACLMIAFRYNVIGNLPTNE
                     QERGYYAAAQKLNQGVSSNSAQVLESLREIYPKDDAFKAMFAEKIIRTTDSRNNRVMR
                     YILCELERQHSGQDYDFESDTFNVEHVLPQSPETDWEQFTDEEVEAMVYRIGNMALLT
                     KGVNKDIGNAAYAVKRPVLQTSGFGLTQKLAEENADWTPERIEARQKSLAKLATSIWR
                     IDTLS"
     gene            complement(2844..>5126)
                     /locus_tag="PKZ49_10550"
     CDS             complement(2844..>5126)
                     /locus_tag="PKZ49_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HND37045.1"
                     /translation="LTATPLQNSLLELYGLVSFIDEHTFGDLKSFREQFANLNQEQVF
                     QTLKARLKAVCHRTLRRQVTAYIPYTKRLPLVEEFTPEDSEDRLYHLVSEYLQRDNLQ
                     ALPSSQRSLITLVLRKLLASSTFAIAGALTSISTRLKRKLNKQEPEESLEDELDQDYE
                     ALDETAEEWTDDEPAELLTEDDRVALEQEITELDAFAALAISIDHNAKGKALLKALGI
                     AFAKAKELGAAEKAIIFTESRRTQNYLLRLLADSPFAEGIVLFNGTNTDDQSKVIYNQ
                     WLERHQGTDRVTGSRTADMRSALVDYFRDQGRIIIATEAGAEGINLQFCSLVVNYDLP
                     WNPQRIEQRIGRCHRYGQKHDVVVVNFLNRKNAADKRVFELLAEKFKLFEGVFGASDE
                     VLGAIEAGVDFEKRIAAIYQHCRMQEEIQAAFDQLQLELSLEINESMTRTRQQLLDNF
                     DDEVREKLKVRDETSKAYLNHYERLLMQLTQYELNGRAEFLDDSSFRLEKQPFPEQAA
                     AIPVGLYELPRRSGEAHLYRLNHPLAETLIVQAKMRNLPPAEIHFDYQQYDGKVTLLE
                     EYIGKSGWLTLSLFSVESLDQAEGHLIFAAKTDDGQILDEEIAARLLTLPGNVVNQVQ
                     IGLENTSLEVQTPKRQLEIQRTISERNARFFEAEADKLDGWADDLKVGLEREIKEIDR
                     QIKEARRTAVTALTLEEKLSGQKLIKSLEAQRNFKRRSLFDAQDQVNKQREELISVIE
                     GKMNQSTRSDQLFSIRWTIS"
BASE COUNT         1250 a         1267 c         1160 g         1449 t
ORIGIN      
        1 taccgccata acctccttct tagcttcctg tatatgtccg acttcttcat gcagccaaat
       61 cgactgtgga acgattcctt ccattacttc agataagaaa cgcttgatac gtggaacatt
      121 gtctcctttc tttccccacc aaatacgtcc atccttgtct agctcagtaa atttttcaaa
      181 agagacgcgc caatacatac ctttcggagg tcctgtaatt acacgtccag aaggtgtagt
      241 aattgggtag ataccagccg agtaggaatt tcgcgcagac atgtctcccg aggtccagag
      301 tcctctggga tcgttgtcaa gattcttata gttcaggttg tgcttgtcgt ctcgtggaag
      361 cagtttcctt ttccattgaa gtttgtcttt cgcatacaac aagacatggt cgtgagagtc
      421 agaaatatat tgagccgtgc tatttggcgc gaaacgtttt tgccacacaa cattcgctac
      481 aaaattcgcc cgcccaaaca cttcatcaca caacaccttg aggtaatgcg cctcgttgtc
      541 atcaatcgtg atccacagcg aacatcctcc gacaacaacc gccgaataat ctccagccgg
      601 tcgcgcatca gcgacagcca gatcgaatgc tccaccccat catcgtaatg cgtaaacgcg
      661 ctgccggtgt tgtatggtgg atcaataaac acgcacttca ccttccctgc gaactctgcc
      721 tccaaagcct tcaatgccag caagttgtcg ccgaagatca gccggttatc gaagatgtcg
      781 ccatctgtca cccgatgctt cgcgtgataa tttctcgccg ggtcttccag caaaatgcgc
      841 ggctccagct tgggccggtt ctccttgccg atccaggtga gttcgagcct ttgctttttc
      901 attcaggagt agccattagg aaagcgtatc aatacgccaa atagaggttg ccaatttggc
      961 gagtgatttt tgccgcgcct cgattcgttc gggcgtccaa tccgcatttt cttctgccag
     1021 tttttgcgtc aggccgaagc cgctggtttg caagacaggc cgcttgactg cataagcggc
     1081 gttgccgatg tctttgttaa ctcccttggt aagcaaggcc atgttgccga tgcggtaaac
     1141 catggcttcc acctcttcat cggtaaactg ttcccaatcg gtttccgggc tttgaggaag
     1201 aacatgttcg acattgaacg tgtcgctctc gaagtcgtaa tcctgtccgc tatgctgccg
     1261 ctccagttcg cacaggatgt agcgcatcac acgattgtta cgtgaatcgg tggtgcggat
     1321 aattttttcg gcgaacatgg ctttgaatgc atcatctttc ggataaattt cacgcaatga
     1381 ttcgagcact tgagccgagt tggaagatac cccctgatta agcttttgcg ccgccgcata
     1441 gtagccgcgc tcctgttcgt tggtgggcag attaccgatg acattgtagc gaaaagcaat
     1501 catcaggcaa gcgcgtaata cggcggtaaa ctggccattc ttcaatgtcc gatgcgcggc
     1561 cagtatcaac gggaatggtt gacgcactcg gaacatgcgc aataattggg catagctttt
     1621 gagcgtgctg tcccaatgcg aggcctcggg attggtcaat gccaaatagg tgtccatatc
     1681 ttcttccatg ccgcgcaaca attcaaaaac cgctgccctg ttgccaactt tattgcgtat
     1741 ggttttgaat aaatcggatt gacgaacaaa cgtattgcgg ctgttccaat ggctgcgcaa
     1801 aaaatccggg aagctttcgg agccaagacg cgaaaccatg gattcccaac gatcttcgag
     1861 cgccttcatt tcattgggat gttcattttg cttgtgcagc accgagaaca agtagttctt
     1921 taacaaatcg gtggatgaca accttacgcc gcgcgcattc aaggtttcaa acaccttata
     1981 ggcattgagt tcgtctgtta cgctgataac ggtaaaaaac aggtgatcac tcatggtttc
     2041 gacgagactg gccaacgcta cgccttcatc ccccccgtgt ttttttgcat aatcccagat
     2101 gcgcttttca aaccactcaa aagctttgcg caagctatgc tcggaagcgc ggaagccccg
     2161 cacaggtaaa tgccctagcg gcacaagata agtctgaaaa taactgtcgt tattgcgatt
     2221 gagcgtcaac ttgctgcggg tgaccagagt taccgggtca agatagccaa tataggtatt
     2281 cctgatttgt tcgagccgct gcttgttttg atctggattt tttccttcac ctatcagccg
     2341 gttgaggttc ttgagcaccg caagcacaat caggcttaat gttgttaggc gttgctgacc
     2401 gtcgatgaca tcaaaatttt tctcatcttg ggattgcaag acaagataac ccatgtagtg
     2461 cgcaggctca ccgccttcct gaatcgtgcc aagaatatcg gaccacagat cttcccactc
     2521 ctcgtcagtc caactatagt cacgctggaa gcgaggaatt tgatacgcca atccattgcc
     2581 aatcaacttc cgataggtgt tattttcagt tttaaaattt gttgctgaca agatttactc
     2641 ctctacattt tcatggccta catcttgaaa agccaaggat tctaccccat ccatctctca
     2701 tggcaacttc cttgcgatac ttgcatgcct ttgcaatcat agacactatc tataattcag
     2761 tgagttatac tcacattttg cagattggac aaataatcat tggttttgtc ataacaatat
     2821 ttctcctgtt tatatagcta tggctatgaa atagtccagc gtatcgaaaa taattgatca
     2881 gatcgagtac tctgattcat tttcccttct atcaccgaaa tcaattcttc acgttgttta
     2941 tttacctgat cctgtgcatc aaagagtgag cgtcgcttaa aattgcgctg cgcctctaac
     3001 gatttgatca gtttttgtcc ggaaagcttt tcttccagag tcagcgcagt caccgcagtg
     3061 cggcgtgcct ctttaatttg gcgatcaatt tctttaattt ctctttccag gccaaccttc
     3121 aaatcatctg cccagccatc cagtttgtct gcctcagcct cgaagaaacg ggcattgcgt
     3181 tctgagatag tgcgctgtat ttccaattgg cgctttgggg tttgtacttc cagggatgtg
     3241 ttttctaaac caatctgaac ttggttgacc acattcccag gaagtgtcag tagtcgtgcg
     3301 gcaatttcct catccaatat ttggccatca tcggtttttg ctgcaaaaat aagatgaccc
     3361 tccgcttgat cgagtgattc aacgctaaaa agcgagagag tcaaccagcc ggatttccca
     3421 atatattctt ctagtaatgt aacctttcca tcgtactgct gataatcaaa atgaatttct
     3481 gctggaggca ggttacgcat tttagcctgt acaatgagtg tttcagcgag tgggtggttg
     3541 aggcgataaa gatgcgcttc accggatcgc cgtggcagtt catataatcc aactgggatt
     3601 gctgcagcct gctccgggaa cggttgtttc tccaagcgga acgaggaatc atccaaaaac
     3661 tcggcacggc cattgagttc atattgggta agctgcatca gcaaacgttc gtaatgattg
     3721 aggtatgctt tagatgtctc gtcccgtact ttgagctttt cacgcacctc gtcatcaaaa
     3781 ttatctaata attgttggcg cgtacgtgtc atagattcat tgatttccaa actcaattcg
     3841 agctggagct gatcaaaagc tgcttgaatt tcttcctgca tgcggcagtg ttggtagatg
     3901 gcggctatgc gcttttcgaa atcaacaccc gcttcaattg cgcccagcac ttcatcgctg
     3961 gcaccaaaaa caccttcaaa gagtttaaat ttttctgcca ataattcgaa aacgcgcttg
     4021 tctgcagcat tcttgcggtt aagaaagttt acaaccacca catcgtgctt ttgcccataa
     4081 cggtgacaac gaccaattcg ttgctcgatg cgctgcggat tccatggcag atcataatta
     4141 acgaccagtg agcagaattg aagattgata ccttcggcac cggcttccgt tgcgattatg
     4201 atgcgtccct gatcacggaa ataatcgacc agtgctgagc gcatatctgc ggtgcgcgac
     4261 cctgttactc ggtcggtgcc ttggtgacgc tcaagccact gattgtaaat aactttggat
     4321 tgatcatcgg tattagtgcc attgaaaagt acaattcctt cagcaaatgg actgtccgcc
     4381 agtaagcgca ataaatagtt ttgtgtgcgg cgtgattcgg taaagatgat tgctttctcg
     4441 gctgcgccaa gttcttttgc cttggcaaat gcaataccca gtgcttttag cagcgcctta
     4501 cccttggcat tatggtctat tgagatggca agcgcggcga aagcatcaag ctcggtaatt
     4561 tcctgctcaa gagccacacg atcatcctcg gtgagcaatt cggcaggttc gtcatccgtc
     4621 cattcctcgg cagtttcatc gagtgcttcg tagtcctgat ccagttcatc ttcgagcgat
     4681 tcttcaggtt cttgtttgtt cagcttgcgt ttgagtcgag ttgaaatcga agttagcgca
     4741 cccgcaatgg caaaggtgga tgaggccagc aatttgcgta acaccagtgt tatcagggaa
     4801 cgctggcttg atggcagcgc ttgtagattg tcacgctgca gatattctga aacaaggtgg
     4861 taaagacgat cttcgctatc ttccggagta aattcctcta ccaacggcag gcgcttggtg
     4921 tatgggatat aggcagtgac ctgacgacgc agggttcgat gacaaaccgc cttgagccga
     4981 gccttgaggg tttgaaatac ttgttcctgg ttcaaattgg caaattgctc gcgaaaactt
     5041 tttaaatcac cgaaagtatg ttcatcaata aaactcacca atccatagag ttccagaagt
     5101 gaattttgta gtggtgtcgc tgtaag
//
LOCUS       DAOQKQ010000178         5109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_8859_length_5109_cov_4.695489, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000178 DAOQKQ010000000
VERSION     DAOQKQ010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5109
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8859_length_5109_cov_4.695489"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="PKZ49_10555"
     CDS             <1..455
                     /locus_tag="PKZ49_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HND37046.1"
                     /translation="FLPNEASCPKFINADLIAAGLSPFAPDVAAMKAARIMLESIKEL
                     EEHGESFAFETTLSGLTYAQRIRRWRKAGYHVTLFFLSLPNSQMAIDRVAERVRQGGH
                     DVPGEVIRRRFAAGLRNFEQVYRDEVDAWALYDNAGDEPALINWGEKA"
     gene            628..3330
                     /locus_tag="PKZ49_10560"
     CDS             628..3330
                     /locus_tag="PKZ49_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HND37047.1"
                     /translation="MNRHVNAIAGRLSLRAPQRRSLEILDRITEITPPRKDRDLIATL
                     EIIRSEFPSVTDFEREFPSLCFALATGVGKTRLMGAFISSLHLVHGINNFFVLAPNLT
                     IYNKLITDFRDRTHPKYIFKGISEFAIDAPAIITGDNYNQHDPVSGRLFGGIRINIFN
                     ISKINSEVRGGKAPRIKRLSEYIGESYFDYLAGLSDLVLLMDESHRYRASAGIRAINE
                     LKPILGLELTATPFVEGTRGAVPFKNVILDYPLGKAMEDGFVKEPAVVTRKNFNPAGM
                     SPEEIERMKLEDGMRLHESVKVELETYARETSNPIVKPFLLVIARDTTHAGQLLALIQ
                     SDGFFEGRYKDKVIQVDSSRTGADEEEMIERLLKVEQPEEPTEIVIHVNMLKEGWDVT
                     NLYTIVPLRAANARTLIEQSIGRGLRPSYGKRTGVTAVDRLNIVAHDKFQEIINEANR
                     PDSAIHLKAVVLDNDELDQKTVTIVSQPQVATQLGIKPVDTTASTQIAGQNVPPLFTK
                     PEEQKVAQIAFEVIRKLENQPRAVPTLAHLKTPEIQAAIVKTVQEQHRPIQLELEGVT
                     ELPDFAAVVAKTVELVTQQTIDIPRILVVPKGEVKAGFRPFILKLDTLKYPAVSDELW
                     IQHLRTSQRDEMTLGAGGIEEKRIEDYVVSGLMDFDDISYDDQADLLYDLAAQTVKHF
                     CSYLSEDEARKVLRCYQRDIAQFIHAQMQDHSWEEAVDYEVVVSKRYTEFKSSAYTHA
                     INEPPADYRVSPADRSNMSKYVFGGFTRCLYPIQKFDSEAERVLATILDRDAIKWFKP
                     AKGQFQIFYKQGADHSEYQPDFVAEANDTIYMLEPKASNQMTDPIVIAKMEAAIKWCK
                     NASDYAMVNGGKPWRYVLIPHNAIAQNMTLEGLAQTERTESGRL"
     gene            3645..4952
                     /locus_tag="PKZ49_10565"
     CDS             3645..4952
                     /locus_tag="PKZ49_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HND37048.1"
                     /translation="MIKRQSVEKLLEFIDDSPTPWHAVATIETALRAAQFIQLDETNN
                     WSLQAGGRYYVIRDDSSIILFVVGQNPLADSGFKIVGAHTDSPGLRIRPNGAQVSNGF
                     AQLGVEIYGGPILATFTDRDLSLAGRVSYIDGQGHIAHKRIRFDQSLLRIPNLAIHMN
                     RSVNDEGLKLHKQTELLPLFAQLTQEQLPKPYFMQLLAQATNLDIDQILSWDLAVYDT
                     QKGVFWGAANEFYANSQIDNLASCHAGLQALLNEQVLNQSQDTLVCAFFDHEEVGSES
                     HIGAAGSFLSDCLQRITLALNLDREDSACALAHSFLISADMAHAYHPNFPNAYDANHK
                     VFVNQGPVIKSNANRRYSSESISIAQFIRWCQDANVPYQHYSHRNDLACGSTIGPLAS
                     AKLGIRSIDIGCPMWAMHSIRESAGVHDHDYLIKVLKQFFLSA"
BASE COUNT         1377 a         1091 c         1291 g         1350 t
ORIGIN      
        1 aatttttgcc caatgaagcg agttgcccaa agttcataaa tgcagacttg attgcagcag
       61 gtttgtcgcc gtttgcaccg gatgtggcag cgatgaaggc ggctcgtatt atgttggaaa
      121 gcattaaaga gcttgaagaa cacggtgaaa gttttgcgtt cgaaactacg ctttctgggt
      181 tgacttatgc gcaacgaatt cgccgctggc gcaaggccgg ttatcatgtc acgctgtttt
      241 tcttgtcgtt gccgaactca caaatggcca ttgatcgtgt ggcggaacgt gtgcgccagg
      301 gtggccatga tgtgccgggc gaggttatcc gcaggcgttt tgcagcgggg ttaagaaatt
      361 ttgagcaagt ttatcgggat gaagtggatg cttgggcgtt gtatgacaat gcaggcgatg
      421 agccggcgct gattaattgg ggagaaaagg catgaaccag aaaagtatag aactggcaaa
      481 agattcggat ttggcgggtt ctttggttgc aatccgccgc gctgctcagc gggcaagaca
      541 attggcggtc agtacgaaca ctgagcttgt cgtcttgcgc gatggtaagt gtgttcatat
      601 aaagccaagc ggagagcaaa agccgcgatg aatcgtcatg tcaacgccat tgccggacgg
      661 ctcagcttgc gtgcaccgca gcgccgttcg ttagaaatat tggatcggat cactgagatt
      721 actccgccaa gaaaagatag ggatctaatt gcgacattgg agattatccg cagtgaattt
      781 ccatccgtca cagatttcga acgcgagttt ccgtccctgt gttttgcact ggctaccggt
      841 gtcggtaaaa cccggttaat gggcgcattt attagttctc tgcatttagt gcacggcatc
      901 aacaattttt ttgtgctggc accgaatttg acgatctaca acaaactgat caccgatttc
      961 agggaccgca cgcatccgaa gtatattttc aaaggtatct cggaatttgc catagatgcg
     1021 cctgccatca ttacgggcga caattacaat cagcatgatc ctgttagtgg caggctattt
     1081 ggcggtatac gcatcaatat tttcaatatc tccaaaatca attctgaggt gcggggtggc
     1141 aaagcgccgc gcatcaagcg attatctgaa tatattggcg aaagctattt tgattatttg
     1201 gctggactat ccgacctggt gcttttgatg gacgaatcgc atcgctaccg cgccagcgcg
     1261 ggaatcagag caatcaatga gttgaagcca atccttggat tagagctgac ggctactcct
     1321 ttcgtagaag gtactcgagg tgcggtaccg tttaaaaacg ttattctgga ttatccattg
     1381 ggaaaggcga tggaagacgg ttttgtaaaa gaacctgctg tcgtgacgcg caagaatttc
     1441 aatccggcag gcatgtcacc agaagaaatc gaacgcatga aattggaaga tgggatgcgc
     1501 ctgcatgaga gcgtgaaggt ggaattggaa acttacgcca gagaaaccag taatcctatt
     1561 gttaaaccat tcctgctggt gattgcaaga gatacgaccc atgctgggca gttattggcg
     1621 ttgatccagt ccgatggctt cttcgaagga cgttacaaag acaaggtcat tcaggttgat
     1681 tcaagccgaa ccggtgcgga tgaggaagaa atgatcgagc ggttattgaa ggtggagcaa
     1741 cctgaagagc caactgagat cgttattcac gtcaatatgc tgaaagaagg ttgggacgta
     1801 accaatctct atactattgt gccgctacgt gctgctaatg cacgtacctt gattgaacaa
     1861 tccatcggtc gtggtttacg gccgtcttat ggtaagcgca cgggcgtaac ggcagttgat
     1921 cggctgaata ttgttgccca tgataagttt caggaaataa ttaacgaagc gaatcgacct
     1981 gattccgcca ttcatctgaa agcagtggta ctggataatg atgaactcga tcaaaagaca
     2041 gtaacgattg tttctcaacc ccaagtagct acccaattgg gaataaagcc agtagataca
     2101 acggccagca cacaaattgc ggggcaaaat gtacctcctc ttttcaccaa accagaagag
     2161 caaaaagtgg cgcaaattgc ttttgaggtc atccgcaagc tggagaatca accgagggcc
     2221 gtgcctacat tagcgcactt aaaaaccccg gaaattcagg ctgctatcgt taagacagtg
     2281 caagaacaac atcgccccat acaactggag ctggaagggg tgacagagct gccggatttt
     2341 gcagccgttg tggctaaaac ggtggaatta gtaacccagc agacaattga tattccacgc
     2401 attctagttg tgcccaaggg agaagtaaaa gcgggtttta gaccattcat tctaaaactg
     2461 gatacattga aatatccagc ggtatccgat gaactatgga tacagcacct gcgcaccagt
     2521 cagcgagatg aaatgactct gggtgccggt ggtattgaag aaaagcgaat tgaagattat
     2581 gtcgtcagcg gcctgatgga ttttgatgat atttcttacg atgatcaagc agacctgctg
     2641 tatgacttgg ctgcgcagac cgttaaacat ttctgtagct atctgtctga ggatgaagcg
     2701 cgtaaggttt tgcgttgcta tcagcgtgac attgcccaat ttatccatgc acaaatgcaa
     2761 gatcatagct gggaagaagc agtggattac gaggtggttg taagtaaaag gtacacagaa
     2821 ttcaaatcta gcgcctacac tcatgcgatt aatgaaccgc cagccgatta tcgggtatca
     2881 cccgcagaca ggagcaatat gtcaaagtac gttttcggcg gttttacacg ttgcctgtat
     2941 ccaattcaga agtttgactc tgaagcagaa cgcgtattgg caaccattct tgaccgtgat
     3001 gcaatcaagt ggtttaaacc ggcaaaggga caattccaga ttttctacaa gcaaggagct
     3061 gaccactctg aatatcagcc ggattttgtt gctgaggcaa atgatacaat ctatatgctc
     3121 gaacccaagg ctagcaatca aatgaccgat ccaatcgtta tagccaagat ggaagctgcc
     3181 attaagtggt gcaagaatgc gtctgattac gccatggtta atggcggcaa gccttggcgc
     3241 tatgttttaa tcccgcataa cgcaatcgca caaaatatga cacttgaagg attagctcaa
     3301 actgaacgga ctgagtccgg tagactataa gttacagttt gcaacgcagc cagctatttt
     3361 gtaaacgtcc aacttctggg gatcagttca gagtcggggc gattcaattc attctttcta
     3421 taaattgctg tctgactatt tgccaactag tggtgatatt tcgtgctacg ctttatcttg
     3481 gaataggatt atgattgagt ctatgtttat gctgattctg cgaggctcag gtaagcacat
     3541 aattgatgca cttgcgacac taacccacca agattagaac tattacaata ttgagtgaca
     3601 ttctttatcc tgcttttatc gtgctggttt attttgctta gatcatgatt aagagacaat
     3661 ctgtagagaa attgcttgag tttatcgatg acagcccaac gccatggcat gctgtggcaa
     3721 cgattgaaac cgcgctgcgt gctgctcagt ttatccagct cgatgaaacg aataactggt
     3781 ccttgcaagc gggcgggcgt tactatgtga tccgcgatga ttcttcgatt atcttatttg
     3841 tagtggggca aaatccgctt gcggattcgg gttttaaaat cgtgggggcg catacggatt
     3901 ctcctggatt gcggatacga cccaatggtg ctcaagtcag caatgggttc gctcaactcg
     3961 gcgttgagat ctatgggggg ccgattcttg cgacgtttac cgatcgtgat ttgagtttag
     4021 cggggagagt cagttatatc gacggacaag gccacatcgc gcataagcgc atccgcttcg
     4081 atcaatcgct gctgcgcata cctaacttgg ccattcatat gaaccgtagc gtcaatgatg
     4141 aggggttaaa gctacacaaa caaactgaat tgctgccact gtttgcccaa ttaacgcagg
     4201 agcaactacc caagccttat ttcatgcagt tattggcaca agcgacgaat ctcgatatcg
     4261 atcaaattct atcttgggat ttagccgttt acgataccca gaaaggtgtt ttctggggcg
     4321 ctgcaaatga attttacgcc aatagccaaa tcgataatct ggcttcttgt catgcagggt
     4381 tgcaagcgtt attgaatgag caggtcctga atcaatctca agatacttta gtttgtgcat
     4441 ttttcgatca tgaagaggtc ggcagtgaaa gccatattgg tgcggctggc agctttttat
     4501 cggattgttt acagcgcatt accttggcgc taaacctcga tcgcgaagat agtgcgtgcg
     4561 cattagctca tagtttcttg atcagtgccg acatggcgca tgcttatcat ccgaattttc
     4621 cgaatgccta tgatgcgaac cataaagtgt ttgtcaatca agggcctgtg attaaatcca
     4681 atgctaatcg tcgttatagc tcggagagca tttcgattgc acaatttatc cgttggtgcc
     4741 aggatgcgaa tgtaccttat cagcattata gtcaccgcaa tgatttagct tgtgggagca
     4801 ccattggacc gctagcttcg gccaaacttg gtattcggag tatcgatatc gggtgcccga
     4861 tgtgggcgat gcacagtatc cgggaaagcg cgggggtcca tgaccatgac tatctgatta
     4921 aggtgctgaa gcaattcttt ttaagtgctt aattaataat taacgtgttg aattaaaatg
     4981 agtaacccca ttcttccttt aaagccagca caactctacc aagcgtgtga tcctagccag
     5041 ttcagcttta gaacaacggc tgaactaggg gatttgactg aaataatcgg tcaaacacgc
     5101 gccatggat
//
LOCUS       DAOQKQ010000179         5018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_9124_length_5018_cov_5.742293, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000179 DAOQKQ010000000
VERSION     DAOQKQ010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9124_length_5018_cov_5.742293"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..455
                     /locus_tag="PKZ49_10570"
     CDS             114..455
                     /locus_tag="PKZ49_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HND37049.1"
                     /translation="MPSKEVYCPVEVTLKVIGGRWKVLIIHQLLNGTMRFNELQRNLI
                     GITHRTLSRQLREMEMHQLIIRNDFNEIPPRVEYSLSPLGYSLKNILFAMDEWGRNII
                     CKNSQGCLQRI"
     gene            840..1259
                     /locus_tag="PKZ49_10575"
     CDS             840..1259
                     /locus_tag="PKZ49_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HND37050.1"
                     /translation="MQLKGSKTEGNLKAAFAGESQANRRYLYFAAKADVEGQNDVAAV
                     FRSTAEGETGHAHGHLEYLESCGDPATGLPFGSSRDNLKTAIAGETHEYTDMYPGMAK
                     TARDEGFTEIADWFETLAKAERSHANRFQRALDGFTD"
     gene            1883..2275
                     /locus_tag="PKZ49_10580"
                     /pseudo
     CDS             1883..2275
                     /locus_tag="PKZ49_10580"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
     gene            2810..3400
                     /locus_tag="PKZ49_10585"
     CDS             2810..3400
                     /locus_tag="PKZ49_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HND37051.1"
                     /translation="MAKISRESLMTLEAYAKTRQQFRTQVIAHKKDRLIALGEHITLL
                     FEDELTIRYQIQEMLRAEKIFDEEGILQELAAYAPLVPDGTNWKVTMMIEYADPKERA
                     ERLAQLIGIEDKVWIEVEGYEKILAIADEDLDRENEVKTSSVHFLRFELSHEQIQALH
                     RGSTLRIGVSHPYYETITEAIKNPIREALLNDLNLP"
     gene            3397..3909
                     /locus_tag="PKZ49_10590"
     CDS             3397..3909
                     /locus_tag="PKZ49_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HND37052.1"
                     /translation="MKLRLVFLSLVSLVSCSSNEKFVDTFDNSKNWVEIQAQLPNYPE
                     TENLLEFDAGPANDMRHYVDAQSIAIGEDSVIRFTLVSKSSEGAENISFEGIRCRTRE
                     RKRYAVGRLNKTWAHSRQPEWQLLNNVGQNHALRELAKFYFCPQDIMVGSQQEAIQAL
                     KAGIHPRVVR"
     gene            complement(3923..4531)
                     /locus_tag="PKZ49_10595"
     CDS             complement(3923..4531)
                     /locus_tag="PKZ49_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HND37053.1"
                     /translation="MNKSDVEILEKKLCYQGFFRMERYALRHRLFNGTWGKPIVRELM
                     ERGHAAAVLPYDPVSDCVVMIEQFRIGAVSAPVGPWLLEIVAGIIERDEPAEQVVKRE
                     AVEEAGCTIHELIPLFDYLVSPGGTSERIALYCGRVEANHIVGGFYGIEDEGEDIKVH
                     VMPFAEALSLLNTGKIAAASAIIALQWLALNRDSVRNRWLTA"
     gene            complement(4583..>5018)
                     /locus_tag="PKZ49_10600"
     CDS             complement(4583..>5018)
                     /locus_tag="PKZ49_10600"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111868.1"
                     /note="catalyzes the formation of glutamate 5-phosphate
                     from glutamate in proline biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HND37054.1"
                     /translation="ARSGAHTVIASGHEREVLVRLAKGEAIGTCLLANTPVLIARKQW
                     LADHLQIRGSVILDSGAIKALVEEGKSLLPIGVIDVKGEFDRGEAVSCMDKDGNELAR
                     GLINYSARETRKIMRQPSQNIEELLGYLDEYELIHRNNLVVL"
BASE COUNT         1524 a         1095 c         1011 g         1388 t
ORIGIN      
        1 ttatctgcat gactgattac cttccgacaa aattatattt gatagagtaa tatcattgga
       61 attaattaca agtacgcaca aaaaagatac cgtcaaaatc aggaaaactc aaaatgccaa
      121 gtaaagaagt ttattgcccg gtagaagtta cactaaaagt aattggaggt cgctggaagg
      181 ttttgattat ccatcaacta ttaaatggta cgatgcgatt taacgaactg cagcgaaatt
      241 taatcggtat tactcatcgc accttaagcc ggcagttgcg cgagatggaa atgcatcaat
      301 taattatcag aaatgacttc aacgaaattc ctccacgcgt cgaatattcg ttatcacctt
      361 taggctattc acttaagaat attctctttg caatggatga atgggggaga aatataatat
      421 gtaagaattc tcagggttgt ttacaaagga tttgattaaa tttagtctaa taaacaaccc
      481 catgccgaaa ccacggggaa tcgcaagtta ataaaaaagc atagcgtaat ttccagaaat
      541 tatctggtct gatctcatta attggcaagc aacaaataga gaaaatctgg atttgtagca
      601 aataagctgc tgcattgaaa cacaaagtat tgtatagatt ctagaggttc tagactcata
      661 aatactctac actctgtagt tgacatcaat cagtctgttc gatagtctca tgcgaaggat
      721 agctagaatc ctattatcat gatcagagat gatttcgatt catctctttt gcctacgaga
      781 ttgcaaatca tagtttgttt gtctattttg attaactgca ttgcaaaaag gagagagtta
      841 tgcaattaaa aggatcaaaa accgagggaa atttaaaagc tgcgtttgcg ggtgaatccc
      901 aagctaatcg tcgctatctt tattttgctg ccaaagctga tgtcgaaggg caaaatgatg
      961 tggctgcggt attccgctct acggccgaag gtgaaacagg ccatgctcat ggtcatctag
     1021 agtaccttga atcctgtggt gatccagcaa caggtctgcc atttggttcc tcacgcgata
     1081 atctcaaaac ggcgattgcg ggtgaaactc acgaatatac cgacatgtat cctggaatgg
     1141 ccaaaacggc acgcgatgaa ggttttaccg aaattgcgga ttggtttgaa acacttgcca
     1201 aagctgaacg ttcccatgca aaccgtttcc aacgggcgtt ggatggtttt actgattaac
     1261 cattttgatc gttaatcgac agtagtctat ttcaaagcaa gccaagcaaa agcctttgct
     1321 tggctttaag aatggaatct agtcaatata taaatattga taaactcggt aatactttat
     1381 ttaccggata atttgtctaa ttagttgtaa tttttctgct atgactactc gcgaaggcag
     1441 cctcgaagcc cctaaacgcc atcccctcaa ttggaagcaa actgattttt ataatgaaga
     1501 tcggctcttt gaggaaatga atcgcgtttt tgatatttgc catggatgcc gacgctgcgt
     1561 taatttatgt accgcattcc caaagttatt tgatttaatc gatgatggga aaaccggtga
     1621 actagacggg gtagaaaaaa aaaccttctg ggaagtagtc gatcgctgct atttatgcga
     1681 tatgtgcttc atgacaaaat gcccttatgt ccctcctcac ccatggaaca ttgattttcc
     1741 ccatttaatg ctgcgcgcta aggctgttaa atataaaaaa caaggtgcgc cattccgtga
     1801 caagttatta gcgaataccg ataccgttgg aaaattagca accattcctg tcgttgttca
     1861 ggtaaccaat acgctcacca aaatgccggt agccagaaaa ttattggaaa acacgttggg
     1921 tattcatgcc gacaggaaat tacccgatta cgcgccacaa aaatttcgta accacgcaca
     1981 acccaattct gatttcattg aaaaagaagg agcgagaact ccgggtaaag ttgccatctt
     2041 tgcaacctgt tatatcaact acaacgaacc caatattggg catgacttac tgcaaatatt
     2101 agcgcataac gaaataccta cttgtttggt tgaacaagaa gtctgttgtg gtatgcctca
     2161 gctagagtta ggcaatttag agaaagtcga agaatataaa aataagaata tcccagttct
     2221 cgccaaactt gctcaagcgg gttatgccat tcttactgcc gtaccttctt gtacgctgat
     2281 gttcaagcaa gaattaccac tgatgttccc tgacgatgaa gcggttcaag cagtcgctgc
     2341 ggctatgttc gatcctttcg aatactttat gttgcgtcat caggatcaac tcctgaaaac
     2401 ggattttacc cgttcactcg gaaaaatttc ttaccacatt ccttgccact tacgcgtgca
     2461 aaatatcggt aagaaaacca gagaccttct acagctcatt ccggatactc acgtaacaac
     2521 cattgaacgt tgctccggtc atgatggtac atggggggtc aaacgtgaat ttttctcaga
     2581 ctccatgaaa atcggtaaac ctgtttttaa ccaaatggcg gcgcccgatc ctgattatat
     2641 ctgctccgat tgcgctattg cagcacgaca tatacagcag ggaattggtc atcatcgtgc
     2701 gcaaaaactt catccgttga cactgttgcg cctcgcttac ggtgaaaata aaccttcatt
     2761 atctgaacca tcgatggttg cccaaccatc ccacgaaaac aaaaattcta tggcaaaaat
     2821 ctcacgcgaa agcctcatga cactggaagc ctatgccaaa actcggcaac aatttcgcac
     2881 tcaggtcatt gcgcataaaa aggatcgatt gatagcactc ggtgagcata taacgctatt
     2941 atttgaagat gagctcacca ttcgctatca aatccaggaa atgcttcgtg cagagaaaat
     3001 atttgatgag gaaggaatct tgcaagaact agcggcttat gctcccttgg ttcctgatgg
     3061 taccaattgg aaagtaacca tgatgatcga gtacgctgac ccaaaagagc gggcagaaag
     3121 gctagcgcaa cttattggaa ttgaagataa ggtctggatt gaagtagaag gatatgaaaa
     3181 aattcttgcg attgccgacg aagatttaga tcgggaaaat gaagtaaaaa catcgtcggt
     3241 tcacttcctt cgctttgaat tgagtcatga gcaaattcag gcattacatc gtgggtctac
     3301 attgcgtatc ggcgtgagtc acccttatta tgaaacaatc actgaagcta tcaaaaatcc
     3361 tatccgtgaa gctttactca atgacctaaa tttgccatga aattaaggtt agtatttcta
     3421 agtttggtca gcctagtttc ttgctcaagt aatgagaaat tcgtcgacac cttcgataac
     3481 agtaaaaatt gggttgagat tcaggcccaa ttacctaatt atcctgaaac agagaattta
     3541 ctcgaattcg atgcaggccc tgccaatgat atgcgacatt atgttgatgc gcagtctatt
     3601 gcgattggag aagatagtgt cattcgcttt acgctcgttt caaaatcttc agaaggtgca
     3661 gaaaatatca gctttgaggg aattcgttgt agaacacgag aaagaaaacg ctatgcggta
     3721 ggtcgcctga ataaaacatg ggcgcattca cgacaacctg aatggcagtt actcaataat
     3781 gtcgggcaga accatgcact acgtgagtta gccaaatttt atttttgtcc acaagatatc
     3841 atggtaggta gccaacaaga agcgattcag gcactcaagg ccggtattca cccgcgtgtt
     3901 gtaaggtgat agtttctgaa ttctatgctg taagccagcg gttacgaacg gaatctcgat
     3961 tcagtgctag ccattgaagt gcaatgatgg cgcttgctgc cgcaattttt ccagtattca
     4021 ataatgataa tgcttcagca aaaggcatta catgaacctt gatatcttcc ccttcatcct
     4081 cgataccata aaaaccacca acgatatgat ttgcctcgac tcgaccgcaa tataacgcga
     4141 ttcgctctga tgtaccgcct gggctcacca gataatcaaa gagcggaatc aactcatgaa
     4201 tcgtacaacc ggcttcttct actgcttctc gcttcacaac ttgttctgct ggttcatccc
     4261 gttcaataat tcctgcaaca atctccagca accagggtcc gaccggtgca gacacagcac
     4321 caattctaaa ctgctcaatc ataacgacac aatcgctcac aggatcatag ggaagaacag
     4381 cagccgcatg tcctcgctcc atcagttcac gaacaatcgg tttaccccag gttccattaa
     4441 acaatcgatg acgcagcgcg taacgctcca ttctgaaaaa accttggtaa cacaattttt
     4501 tctcaaggat ttcgacatcc gatttattca tggatcaatt ctttccaaac aattgaagat
     4561 taggagaagc ttatcaaaaa gattagagaa ccactaaatt gttgcgatgg ataagttcgt
     4621 attcatctag ataaccgagt agttcttcaa tattttggct gggttgtcgc atgattttgc
     4681 gtgtttctct cgcactataa ttaattaaac cgcgcgctag ttcgttccca tctttatcca
     4741 tgcatgaaac tgcctctccc cgatcgaatt ctccctttac atcaatcacg ccaatgggta
     4801 ataagctctt accctcttca actagggctt taattgcgcc actatctaga atcacacttc
     4861 cacgaatctg aagatggtct gccaaccatt gtttccgggc aattaacacg ggtgtattag
     4921 ctagcaaaca ggtcccaatc gcttcacctt ttgctaaacg gactaggact tcacgctcgt
     4981 gtcctgatgc aatcactgta tgcgccccac ttctggca
//
LOCUS       DAOQKQ010000180         5017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_9127_length_5017_cov_5.258565, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000180 DAOQKQ010000000
VERSION     DAOQKQ010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9127_length_5017_cov_5.258565"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            368..1078
                     /locus_tag="PKZ49_10605"
     CDS             368..1078
                     /locus_tag="PKZ49_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HND37055.1"
                     /translation="MIRLYQFNRTWKIPNLSPFCCKVETYLRMADITFEIKATLPSNA
                     PKGKLPYIEDGDLSLGDSAFIIAHLKQHYQDLDQSLDSIEQSISVAMQRLIEEDLFWV
                     ALFSRWQYTEANWQVNKEAIFGSIPPLVRDLVAAFYRRKIKQQIYGHGMGRHTASEIF
                     TRGNQDIDVLSTFLGNKTYFCGNLPTTLDASAFGMLVNIIQCPIESPLKAHALTKQNL
                     VEFVEKILHRYYLDLKTA"
     gene            1101..1790
                     /locus_tag="PKZ49_10610"
     CDS             1101..1790
                     /locus_tag="PKZ49_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HND37056.1"
                     /translation="MSHTENHRSHRTGWLRAAVLGANDGIVSIASLVIGVAAAHASRE
                     AILLAGIAGLVAGAMSMAAGEYVSVSSQADTEQADIILEQRHLNDDLEFEFQELKAIY
                     IQRGLTPELAQQVTKQLMAHDALGTHLRDELGIQTQLGAKPIQAAMTSAAMFTLGASM
                     PLVASWIAPENFIIWLVASVSILSLTCLGALAAYLGGANIITGSLRVTFWGALAMLVT
                     AITGKLFGIII"
     gene            1863..2969
                     /locus_tag="PKZ49_10615"
     CDS             1863..2969
                     /locus_tag="PKZ49_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003984083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HND37057.1"
                     /translation="MKTRAAVAWQAGKPLTIEEVDLMAPQAGEVLVEIKATGICHTDY
                     YTLSGADPEGLFPAILGHEGAGIVIDKGPNVKSLQIGDHVIPLYTPECRECKFCLSRK
                     TNLCQAIRSTQGRGLMPDATSRFSLNDQPIFHYMGTSTFSNYIVVPEIALAKIRKDAP
                     FDKVCYIGCGVTTGIGAVVFTAKVEAGANIAVFGLGGIGLNVIQGAKMVGADKIIGID
                     INPARESMAREFGMTHFINPNDTENIVDAIVQLTDGGVDFSFECIGNTKVMRQALECT
                     HKGWGRSIIIGVAEAGAEISTRPFQLVTGRKWEGSAFGGARGRTDVPKIVDWYMEGKI
                     KIDPLITHTLKLDDINEGFELMKAGQSIRSVVIF"
     gene            3001..3960
                     /gene="speB"
                     /locus_tag="PKZ49_10620"
     CDS             3001..3960
                     /gene="speB"
                     /locus_tag="PKZ49_10620"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HND37058.1"
                     /translation="MVDKIKGDGAIRREGLYGSSIENAYAGVLSFLRRKYTRDLTGAD
                     IVVSGIPLDLATTFRPGTRFGPQAIRAASVQLVELRPYPWGFDPFDALAVIDYGDCWF
                     DAHNPLTIKPTIVEHAQRILATNARMLTFGGDHYISYPLLIAHAQKYGTPLALLHFDA
                     HCDTWPDNGPESLDHGTMFYKAIKENLINPSISAQVGIRTWNDDFLGMNIIDAPWVHD
                     QGVNATIKKILEIIGTNPVYLSFDIDCLDPAFAPGTGTPVPGGLTTDQALRIIRGLGS
                     LNIIGMDIVEVSPAYDHSSVTALAAAHIACDLLCLMRNQKLKI"
     gene            3966..4817
                     /gene="fghA"
                     /locus_tag="PKZ49_10625"
     CDS             3966..4817
                     /gene="fghA"
                     /locus_tag="PKZ49_10625"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HND37059.1"
                     /translation="MTLPFEMRSTHRCFGGLQGFYQHRANSLGGLPMRFSVYQPPQAN
                     ANRVPVLYFLAGLTCTEETFMIKAGAQRLAAQYGYMLVSMDTSPRQTAIAGEADDWEI
                     GSGAGFYLDACLEPWSKFFHMESYVTKELYQLILEHFPADPTRIGIFGHSMGGHGALI
                     LALRNPSLYQSVSAFAPITAPSDCPWGQKAFNCYLGEAKDRWIQHDATALIAAGHKVP
                     TLLIDQGLNDPFLATQLNIDRFETTCQLAKQPLILRRHEGYDHGYYFISTFIEDHFKH
                     HETLLGG"
     gene            complement(4823..>5017)
                     /locus_tag="PKZ49_10630"
     CDS             complement(4823..>5017)
                     /locus_tag="PKZ49_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HND37060.1"
                     /translation="KQLNMESVAEGVEDRQDWDFLRQTGCDLAQGNFISKPLLASEFG
                     DWITYWQQRVKADLMVDNLS"
BASE COUNT         1408 a         1120 c         1051 g         1438 t
ORIGIN      
        1 acaccctaca ggctagtcac cttatccatt ttagtcaagt agtttttcca tcacctcact
       61 agggatttat aaaaacctta agaaaatcat aacaatatga gggttactct ttctgagttc
      121 aactagcttg aaatttatta accccaaacc ttttacaaag acctcacaaa gttatttaag
      181 acaaatcaaa tcttccatag aaaaaaatta gaaaatacta aagatcctga ttaacgaata
      241 aaaactgccg gcctagaatc atcggaaaaa taaccaaaac ctagagacct gaggctatca
      301 actaaaaata cacatctact gtaaatgaag cgtatattgt ctaatttagg tatctagatt
      361 actgatcatg ataagacttt atcaatttaa ccgtacttgg aaaattccta atcttagccc
      421 cttctgctgc aaggttgaga cctatctccg catggctgat atcactttcg aaataaaagc
      481 aacgctacct tccaatgcac cgaaagggaa actgccctat attgaagatg gtgatctctc
      541 actcggtgat tctgctttta tcattgctca tttgaaacag cattatcaag atctcgatca
      601 atctctagac tccatagagc aatcaatttc agtcgcaatg cagcgactca tcgaagaaga
      661 tttattttgg gttgccttat tttcacgctg gcaatatacc gaagctaatt ggcaagtgaa
      721 taaagaagct atttttggaa gcattcctcc cctagttcgt gatctcgtcg cagcctttta
      781 tcgccgtaaa atcaaacaac aaatctatgg tcatggtatg ggtcgacaca ccgcctcaga
      841 aatttttact cgtggtaatc aagatatcga tgtactgtcg acctttttag gtaacaaaac
      901 ttacttttgt ggaaatctgc ccacaacatt agacgctagc gcttttggaa tgctagtcaa
      961 tattatccaa tgcccgatcg aatcgcccct taaagcacac gcattgacta agcaaaattt
     1021 ggttgaattt gttgaaaaaa tattgcaccg ttactatctc gatttaaaaa cagcctaaat
     1081 tctttgattc acaaaacacc atgagtcata ctgaaaatca tcgctctcat cgaacaggtt
     1141 ggctacgcgc cgcggtcctt ggagcaaatg atggtattgt atccattgca agcctcgtga
     1201 tcggtgtagc tgctgcccac gctagtcgag aagccatatt actagcaggt atcgcaggtt
     1261 tagttgccgg tgccatgtcc atggccgcag gagaatacgt ttcagtcagt tcgcaagctg
     1321 atactgaaca agcagatatt attctagagc aacgccacct caacgatgat ctagaatttg
     1381 aatttcaaga attaaaagcg atttatattc aacgaggatt aacgcctgag ttagctcagc
     1441 aggttactaa gcaattgatg gcacatgatg cactcggcac tcatcttcga gatgagttag
     1501 gtattcaaac ccaattaggt gccaaaccca tccaggccgc aatgacttcg gctgctatgt
     1561 ttacactcgg cgcatcaatg cccctcgttg cctcatggat cgcccctgaa aatttcatta
     1621 tctggttagt tgccagcgtc tcgattcttt cactcacatg cctgggtgct ttagctgctt
     1681 atcttggtgg tgcaaatatc attactggta gtctgcgagt caccttctgg ggcgcattgg
     1741 caatgctcgt tactgccatt acgggcaagt tatttggtat tattatctag cgctgacgca
     1801 attccaagct ataacattgc atctcattta atatgatttg actaagaaag gaaatattcg
     1861 ctatgaagac ccgcgctgcg gttgcttggc aagcaggcaa accactgacg attgaagaag
     1921 ttgacttaat ggcaccccaa gccggcgagg tgctagttga aattaaagca actggtattt
     1981 gccacactga ttattacaca ctatctggcg cagatcctga aggtttattc cctgcaatac
     2041 tggggcatga aggtgcaggt attgtcattg ataaaggtcc taacgttaaa tcactccaaa
     2101 ttggtgatca tgtcattcca ctttatacgc cagagtgccg agagtgtaaa ttttgtttat
     2161 ccagaaaaac caacttatgt caggcaattc gtagcacaca aggacgtggc ttaatgccag
     2221 atgctacttc acgattttca ttaaatgatc aacctatttt tcactacatg ggaacgtcta
     2281 cgttctcaaa ttatatcgtc gtccctgaaa ttgctctggc gaaaatccga aaagatgctc
     2341 cctttgacaa agtatgttat atcggctgtg gcgttaccac tggaattggt gctgtagtat
     2401 ttaccgcaaa agtagaagca ggggcgaata ttgctgtatt tggtttaggt ggaattggtt
     2461 taaatgtcat ccaaggcgct aagatggtgg gcgcagataa aattatcggt atcgacatta
     2521 atccagccag agaatccatg gccagggaat ttggaatgac gcacttcatc aatccgaacg
     2581 acactgaaaa tattgtcgat gccattgtac aactcactga tgggggagtc gattttagct
     2641 tcgaatgtat cggtaacact aaagtgatgc gccaggcatt ggagtgtacc cacaaaggtt
     2701 gggggcgaag cattattatt ggtgttgctg aagcgggtgc tgaaattagt actcgcccat
     2761 tccaattggt aacgggtaga aaatgggaag gatccgcttt tgggggcgca cggggacgaa
     2821 cggatgtacc taaaatcgtc gattggtata tggaaggcaa aatcaaaatt gatccactca
     2881 ttacccatac cctcaaactt gatgatatca atgaaggctt tgagctgatg aaagccggtc
     2941 aatcgattcg ttctgttgtg atcttttaat cgtaaaccgt ttttacgcaa tcgagtctaa
     3001 atggtcgaca aaattaaagg ggatggcgca attcgtcgtg aagggctcta cggaagctcg
     3061 atcgagaatg cttatgctgg agtgctgtct tttctacgcc ggaaatatac tcgtgattta
     3121 actggtgctg atattgtcgt ttccggaatc cctttggatt tagcgacgac tttccgtccc
     3181 ggcacacgct ttgggccaca agcgatccga gccgcaagtg tgcaactcgt tgagctgcgc
     3241 ccctatcctt ggggattcga cccatttgat gcgcttgcag tcattgatta tggtgattgc
     3301 tggttcgatg ctcataatcc actcacgatc aaaccgacca ttgttgagca cgctcaacgt
     3361 attctagcaa ccaatgccag aatgctcaca tttgggggtg atcattacat ttcttaccca
     3421 ttgctgattg ctcatgcaca gaaatatggt accccgttgg ctttacttca ttttgatgcg
     3481 cattgcgata cgtggcctga caatggacca gaaagtctcg accacggcac catgttttat
     3541 aaagctatca aagaaaactt gatcaatcca agtatttcag cccaggttgg aattcgtact
     3601 tggaatgatg attttctagg tatgaatatc atcgatgccc cttgggtgca tgatcagggt
     3661 gtcaatgcta ccattaaaaa aattctagag attattggaa cgaatcctgt ctatctcagc
     3721 tttgatattg attgccttga tcctgccttt gcaccgggca ctggcacacc ggtacccggt
     3781 ggactaacaa ccgatcaagc acttagaatc attcgtggtc tgggttcttt gaatattatt
     3841 ggtatggata tcgttgaagt ttcccctgca tatgatcata gtagcgtgac tgcacttgca
     3901 gcagcacata tcgcatgtga tcttttgtgc ttgatgcgca atcaaaaatt aaaaatttaa
     3961 ttccgatgac ccttcctttt gaaatgcgta gtacgcatcg ttgctttggt ggtttacaag
     4021 gcttttatca acaccgagct aattcactcg gtggactacc catgcgattc tcggtctacc
     4081 aacctcccca ggcaaatgca aatcgagtcc cagttcttta ttttctagct ggacttacct
     4141 gcacggagga aacctttatg atcaaagcag gtgcgcaacg actagctgca caatatggat
     4201 atatgctcgt tagcatggat accagcccgc gtcaaactgc aattgctggt gaagcagatg
     4261 actgggaaat tggctcggga gcaggattct acctggatgc ttgtttggaa ccctggtcta
     4321 aattcttcca tatggaaagt tatgtaacca aggaactgta tcagttgata ttggaacatt
     4381 ttccagcaga tccaacgcgt atcggtattt tcggtcattc gatgggagga catggcgctt
     4441 tgatccttgc attacgtaat ccgagtttat accaatctgt atctgcattt gcccccatta
     4501 ccgcaccttc cgattgccca tggggccaaa aagcttttaa ttgctatctt ggagaagcaa
     4561 aggatcgctg gattcaacat gacgccacgg ctttaatcgc agcaggccat aaagtcccta
     4621 cgctattgat tgatcaagga ctaaatgatc cttttctcgc gacacaactc aacattgacc
     4681 gttttgaaac aacctgtcag ttagccaaac agccgcttat cctgcggcgc catgaaggct
     4741 acgatcacgg ctattatttc attagcacat ttattgaaga tcatttcaag catcatgaga
     4801 ctttacttgg tggatagctt gactaactca gattatccac catcaaatcc gcttttacgc
     4861 gctgctgcca atatgtgatc caatcaccaa attctgaagc tagtaaaggc tttgaaataa
     4921 aattaccttg tgcgagatca caaccagttt gacgcaagaa atcccagtct tgtcgatctt
     4981 caacgccttc tgcaaccgat tccatattga gttgctt
//
LOCUS       DAOQKQ010000181         4935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_9384_length_4935_cov_6.460656, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000181 DAOQKQ010000000
VERSION     DAOQKQ010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9384_length_4935_cov_6.460656"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PKZ49_10635"
     CDS             <1..396
                     /locus_tag="PKZ49_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HND37061.1"
                     /translation="GGGGLSGGQKQRIGLARSMYGDPSLIVLDEPNSNLDDIGEQALL
                     AAIVDLHKRGKTIILITHRTSIITATSKLLFLRDGVIQMFGPSSQVLAELAKQQQQAQ
                     QAMQAKRQAMDQNVQSIALTPGNQPEGKA"
     gene            411..1754
                     /locus_tag="PKZ49_10640"
     CDS             411..1754
                     /locus_tag="PKZ49_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HND37062.1"
                     /translation="MNSVNQNNSINEGVDQATEVKTDASSHTRLGWWIVLAGVGGFLL
                     WATLAPLDKGVPLSGSVMVATNRKAVQHQTGGIVDDILVKEGDTVKAGQVLVKMNDIQ
                     VKAQAEITRGQLITARAVEARLIAERDKKSEIDFPPFLLTALDDPRAMSSISLQKQLF
                     FSRRSVLKHEIEALDESIAGMNLQVRGLEDARKSKEEQRHFLKEQLINMRELAQEGYV
                     PRNRVLELERLHAQLNSDLSTDIGNIGRLRRQISEFNLKRLQRLEEYQREVRQQLTDV
                     QREAESLNSRLLAQDFELANVLVKAPVDGVVVGINVFTRGGVIGPGFRIMDIVPSEDM
                     LVIEGQVPVHLIDKVHAGLDVDFIFTAFNQNKIPRVPGVVTHVSADRLTEERTGIPYY
                     NLKAKVVPEGMKLLTDFQIRAGMPVEIFVKTGERPLMSYLLKPFLDRLHKSLVED"
     gene            1757..3109
                     /locus_tag="PKZ49_10645"
     CDS             1757..3109
                     /locus_tag="PKZ49_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HND37063.1"
                     /translation="MLLFSYKQIRLLMLLAVIGGHVTIANAIGLLDAYESALMHDPTF
                     RAAIHENEAGQQAIAIGRAGLLPTLSITHLQSISRGTQTNPNIAGISEKSNLSFNSEV
                     TTLTLRQPLLNMEAVTNYDQSHARANSSQAKLIGQSHLLIVRLVEAYVKTLLAQDRLY
                     LAKAQDKALEELKIVNQNMLKRGEGTHTDVLETQSRHALAQAQVIEAQDEFDTAKLAL
                     SAMIGQDVAALDALSSHFKVQPLYPADFESWQEMALVGNAELLTQQHMVTSSKLEIQK
                     SRAGHAPRVDLVASLNRNNAASFLFPDRDATFGTIGVEVNLPLYAGGRVIATTDQAQA
                     NHARAGAEFDAIRDRVLVELRKQFQLAQSSIKRIDSLELAVDSALLLVQATDKSIKGG
                     VRINLDLLDAQRQLFLAQVNLAEAKYNYLLAYLRLRLASGTIALDDLEKVAAYFVAKN
                     "
     gene            complement(3135..3602)
                     /locus_tag="PKZ49_10650"
     CDS             complement(3135..3602)
                     /locus_tag="PKZ49_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HND37064.1"
                     /translation="MMHTNMTIDQRILDRRSTIPFLCPYQLGLKQGKRVLKRRSGSGA
                     AYVDTYSTYIVLCTILTIILSALDALLTLNILARGGVELNNFMAVLIDDSTEKFVSIK
                     LALTSLAMIFLLIHHHVELTQSLKVKHLLILILVGYSTLICYEAGILFFASKY"
     gene            3862..3996
                     /gene="rpmH"
                     /locus_tag="PKZ49_10655"
     CDS             3862..3996
                     /gene="rpmH"
                     /locus_tag="PKZ49_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HND37065.1"
                     /translation="MKRTYQPSVIRRKRTHGFLVRMRTRSGAAVIRARRAKGRARLSV
                     "
     gene            4406..4615
                     /gene="yidD"
                     /locus_tag="PKZ49_10660"
     CDS             4406..4615
                     /gene="yidD"
                     /locus_tag="PKZ49_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HND37066.1"
                     /translation="MRQLLIKIIALYQYCISPLLPPSCRYSPTCSMYAKEALLKYGVM
                     KGIVLSIKRILRCHPWSRGGYDPVP"
     gene            4705..>4935
                     /locus_tag="PKZ49_10665"
     CDS             4705..>4935
                     /locus_tag="PKZ49_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HND37067.1"
                     /translation="METKKLVLIFIFITSLLFIWEGWMRERERLDNLANGTLVTDNNS
                     VSQQGPDSPPIPGDALVSSQSNQDRASGIDAAS"
BASE COUNT         1461 a          911 c         1148 g         1415 t
ORIGIN      
        1 gggggggggg ggttatcagg cggtcaaaag cagcgtattg gattagcgcg ttcgatgtat
       61 ggcgatcctt cgctgatcgt gcttgatgaa cctaattcta atctcgatga tattggtgag
      121 caggcgttat tggcagcaat tgtcgattta cataaacgtg gcaaaacgat tattttgatt
      181 acacatcgta ccagtataat tacggcgacg agtaaactat tattcctgcg agatggtgtg
      241 atacagatgt ttgggcctag cagccaggtt cttgctgaat tagcgaagca gcagcaacaa
      301 gcacagcaag caatgcaagc caagcgtcaa gcaatggacc aaaatgtgca atctatcgca
      361 ttaacaccgg gtaaccaacc agaagggaaa gcgtagtttt taatttcact atgaattcag
      421 ttaatcaaaa caattcaatc aatgaaggcg tagatcaggc taccgaagta aaaacagatg
      481 cgtcatctca tactcggctc ggatggtgga ttgttttagc tggggtgggc ggttttctat
      541 tgtgggcaac ccttgcgcca cttgataaag gcgtgccact ttctggatct gttatggttg
      601 caaccaatcg gaaagccgta cagcaccaaa cagggggtat cgttgatgat atcctcgtta
      661 aggaaggtga tacagtgaaa gccggtcaag ttttggtgaa aatgaatgat atccaagtca
      721 aggcacaggc tgaaattaca agaggtcaac ttattactgc tcgggctgtc gaagcccgtt
      781 tgattgctga acgggataaa aaatctgaaa ttgattttcc gccgttttta ttaactgcgc
      841 ttgatgatcc tcgtgccatg agtagtattt cgcttcaaaa gcaattattt ttttctaggc
      901 gctctgtgtt aaagcatgaa atagaagcat tagatgaaag tattgctgga atgaatctac
      961 aggttagagg tttggaagat gctcgtaaaa gtaaggaaga gcaacgtcac tttttgaagg
     1021 aacaactaat taatatgcgt gagttggcgc aagaaggtta tgttccccgt aatcgggttt
     1081 tggagcttga gcgtcttcat gcgcaattga atagtgatct ttcaacagac atcggaaaca
     1141 ttgggagact ccgtcgacag atttcagaat tcaatttaaa gcgcctacag cgcctagaag
     1201 agtatcaacg ggaagttcgt cagcaattga ccgatgtgca acgcgaagcg gaatcattga
     1261 atagccggtt acttgcacag gattttgagt tagctaatgt gttagtaaaa gctcctgttg
     1321 atggggttgt agtcggtatt aatgtgttca ctcgtggggg tgttattggt cctgggtttc
     1381 gcatcatgga tattgtacct tctgaggata tgctcgtgat cgaagggcaa gttcccgtcc
     1441 atttaatcga taaagtgcat gcaggacttg atgtcgattt catttttact gcgtttaatc
     1501 aaaacaaaat tccacgagta cctggagtcg taacccatgt atccgctgat cggttgactg
     1561 aagagcgtac cggaattccc tattataatt taaaggcaaa agttgttcct gaagggatga
     1621 agttgctgac tgattttcaa attcgagcag gtatgccagt tgagattttt gtgaaaacgg
     1681 gggagcgacc gttaatgagc tatttgctaa agcctttttt ggaccgactc cataaatcat
     1741 tggtggaaga ttaatcgtgc tgttgttttc ttataaacaa attcggttac ttatgctgct
     1801 tgctgtgata ggggggcacg ttaccattgc aaacgcaatt ggtttattgg atgcgtatga
     1861 gtcagcctta atgcatgatc caacttttcg tgcggctatt catgaaaatg aagccggaca
     1921 gcaagcaatt gcaatcggtc gggcaggact attacccact ttatcgataa ctcatctgca
     1981 gagtataagt cgaggcacgc aaacgaatcc taatattgca gggattagtg aaaaatctaa
     2041 tctaagtttt aatagtgaag tcacaacatt aacattgcga caacccttgc tgaacatgga
     2101 ggctgttacc aattatgatc aaagccatgc ccgagcaaac tctagccaag ctaaactgat
     2161 tggtcaaagt cacttgctca tcgtccggtt ggtagaagct tatgtgaaaa cattgctcgc
     2221 gcaagatcgc ttatatttag caaaggcgca agataaagcg ctagaagaat taaaaattgt
     2281 taatcaaaat atgttgaagc gcggtgaagg gacccatacc gatgtgctag aaacacaatc
     2341 gcgacatgct ttagcgcaag cacaagttat cgaagctcaa gatgagtttg atacagctaa
     2401 attggcatta tcagcaatga tagggcaaga tgtcgctgct ttggacgctt tatccagcca
     2461 ttttaaagtg cagccattgt atcccgctga ttttgaaagc tggcaagaaa tggcactggt
     2521 aggtaatgct gagcttttaa cacaacaaca tatggttact tcgagtaagc tagaaataca
     2581 aaaaagtcga gctgggcatg ccccccgtgt ggatcttgta gcaagcttga accgtaacaa
     2641 tgctgcttct tttctttttc ccgatcgtga tgcaacgttt ggaaccattg gcgtggaagt
     2701 taatttgcca ctttatgcgg gcggacgtgt tattgcgaca acggatcaag cccaagcgaa
     2761 tcatgcaaga gcaggtgcag agtttgatgc gatccgagat cgagtgctgg tcgaattacg
     2821 taaacaattt caattagcac aaagcagtat taagcgaatt gattctctcg aattagccgt
     2881 agattcagcg ttattgcttg tacaagcgac ggataaaagc attaaaggag gcgtgcgcat
     2941 caatcttgat ttgctggacg cacaaaggca gctattttta gcgcaagtta atcttgcaga
     3001 agcaaaatat aactaccttt tggcctactt acgacttcgc ctggcgtcag gaactatcgc
     3061 tttagatgac ttggagaaag ttgctgccta ctttgttgca aaaaactagc ctactttagt
     3121 gcttataatt tcgattaata tttacttgca aaaaatagta ttcctgcttc gtaacaaatg
     3181 agcgtactat agccgactag gattagtatc aacaaatgct taacttttaa tgattgtgtc
     3241 aattcgacat gatgatgaat caagaggaag atcatcgcaa gcgatgtaag tgctagcttg
     3301 atgcttacga atttctcagt actatcatca ataagtaccg ccatgaagtt attaagctct
     3361 acgccccctc tggctagaat attaagcgta agtaaagcat ctaatgcgct gaggatgatt
     3421 gttaaaatgg tgcaaagcac gatgtaagtg gaataggtat caacataagc agcgccactg
     3481 cctgagcgtc gcttaagaac gcgttttcct tgcttaagac ctaactgata ggggcaaaga
     3541 aagggtattg tagaacggcg gtctaaaatt ctttgatcaa tagtcatatt tgtatgcatc
     3601 atagcttata acctttcaat aattaacaat tagtcctgct aaactgggta agaaacgaaa
     3661 catgattatt cttatctgtg ggttcaggaa caactgtact catgtacaag ctaagtgaat
     3721 tgatcgtgtc gtcttaggat aaagcttgct gtaattaatt gggtgaaatt aaggttgaca
     3781 tcactacctt taaaaggttg taatatactg ttacagaaat gaagtttata tttatttgat
     3841 caactaaaaa gaaagtttaa gatgaaacgc acttatcaac cctcagttat cagaagaaaa
     3901 cgtacgcatg gtttccttgt acgtatgaga acccgtagtg gcgctgccgt tattcgagca
     3961 cgtcgagcaa aaggtcgtgc acgtttaagt gtctaggcct cgcgcttaaa gtcagtataa
     4021 agtagaaaag aagaatcctt tatcgaatgg ttgggtgaaa gagaatcttt tcatttccca
     4081 aaaaatttag gcttcatcgg gcgagagagt ttcaatcggt tattcagttt aaacaatctg
     4141 taagttccca gctattgcag ctatacttga agccgaataa aagtgatttt cccagacttg
     4201 gtttggttgt tgcgagaaaa gtagcgcgga ctgccgtgag acgaaataaa ttaaagcgag
     4261 tgctgagaga ggtttttcgt ttgcacaggc ctaagttagg gaataacgat tatattttta
     4321 gattgcgaaa tctacctacg ggtataaaga ttgttcctgc tggtatcgca aaagaagctg
     4381 agatactgat attgaagtca aggcaatgcg acaattgtta atcaaaatca ttgcccttta
     4441 tcagtattgt attagtcctt tattaccgcc ttcttgccgc tatagtccga catgttcaat
     4501 gtatgcaaag gaagcgctgc taaaatatgg cgttatgaag ggaatagtat taagtattaa
     4561 gcgcattttg cgttgccatc cctggagtcg gggtggttat gatcctgttc cctgaatcct
     4621 gaaaggtaat aaaattttaa taataatgat cgacttcctg ttatgggaag gtttatcgta
     4681 aaaaaataac acgtttaatt taagatggaa acgaagaagc ttgtattgat ttttattttt
     4741 atcacttcat tgctgtttat ttgggaaggc tggatgaggg aacgggagcg attagataat
     4801 cttgctaacg gtaccttggt aactgacaat aattccgttt cccaacaagg ccctgattca
     4861 cctccaatac ccggtgacgc actcgtctct tctcagtcaa atcaggatcg cgcatcaggg
     4921 attgatgccg cttca
//
LOCUS       DAOQKQ010000182         4934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_9389_length_4934_cov_5.679852, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000182 DAOQKQ010000000
VERSION     DAOQKQ010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4934
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9389_length_4934_cov_5.679852"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PKZ49_10670"
     CDS             complement(<1..397)
                     /locus_tag="PKZ49_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HND37068.1"
                     /translation="MNWSQIFMIVLLTMLITIGGTYWVLKNYVFTTRFTPVELNEKEE
                     KSLHEKLLSIGFLDENTDRPDSTNDQEIDHEGFLKPEAYSEHNAPREIIFTERELNAL
                     LAKNTDLAQKLAIDLANNLVSAKLLIPLEE"
     gene            complement(473..1198)
                     /locus_tag="PKZ49_10675"
     CDS             complement(473..1198)
                     /locus_tag="PKZ49_10675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HND37069.1"
                     /translation="MHVSIIIPAFNEERLIESCLHSIREALSFNQHFNFTHEIIVVDN
                     NSTDSTASLAKAAGAHVVFEPINHIARARAAGAQAARGDWFVFLDADCLMSKELLNDI
                     FQLIQSGKYVAAGSALYMRNLTWWAKLLLKTWTVLSVTFKWAAGALMVCNAQAYREVG
                     GFNLNLYAAEEISFSQQMKQWGRAHRLKFTILTAHPIETSSRKIHLYSAREVFAQFLS
                     LVLHPYQTLHSKKKLSMWYDGRR"
     gene            1297..1746
                     /locus_tag="PKZ49_10680"
     CDS             1297..1746
                     /locus_tag="PKZ49_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HND37070.1"
                     /translation="MIWKPNVTVAAVIERERKFLLVEEDQQDLGVQLNQPAGHLEPSE
                     SIIQGCVREVLEETAYTFQPQWLIGIYQWHSTATDTTYLRFALSGFVTEFDANQKLDV
                     GILRASWFDLEEIQRRKSHHRSPLVLRCIEDYLAGKRYSLDIFTHQT"
     gene            1756..2832
                     /gene="mnmA"
                     /locus_tag="PKZ49_10685"
     CDS             1756..2832
                     /gene="mnmA"
                     /locus_tag="PKZ49_10685"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HND37071.1"
                     /translation="MKKQRIIVGMSGGVDSSVAALLLKKQGHEVIGLFMKNWEDDDSD
                     EYCSSRQDFLDAASVADIIGIPLDVVNFSAEYKDRVFSNFLSEYKAGRTPNPDVLCNA
                     EIKFSAFLDHALQLNAEAIATGHYAQVRKVGTLFQLLKGEDGRKDQSYFLYRLNQDQL
                     AKTCFPIGHLYKREVRQIASDSKLPNFAKKDSTGICFIGERPFREFLNRYLPHEPGEI
                     QTIDGQVIGQHIGLMFYTIGQRQGLGIGGVRDSDESPWFVCRKDIEKNRLIVVQGHDH
                     PALLSDSLQAMNLSWISGTAPHQNWVYAAKTRYRQTDAPCSISRIDALSCQIDFAQPQ
                     WAVTPGQSVVIYESKVCLGGGIIN"
     gene            complement(2829..3446)
                     /locus_tag="PKZ49_10690"
     CDS             complement(2829..3446)
                     /locus_tag="PKZ49_10690"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HND37072.1"
                     /translation="MKLIGSLTSPYVRKVRIVLAEKQINYDFEVDVPWNAQTNVSHHN
                     PLGKIPILIVDEKTSLYDSRVIAEYIDSLETQPGMIRLIPESGNTRWQVKRWEALADG
                     ICDAAATIFLERKRPEVQQSEEWIMRQQKKVDWGLIEAAKMLGEGDWCAGKSISLADV
                     ALGCALGYLSFRFPQIEWPRSYPNLNRLAEKLAQRASFIATAPRD"
     gene            3698..4153
                     /locus_tag="PKZ49_10695"
     CDS             3698..4153
                     /locus_tag="PKZ49_10695"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HND37073.1"
                     /translation="MHKLTTYLKVVSRSRDHQRLFLNVNEIMRLQKTLSRKLPPQLAK
                     RCCIGSCSNGALVIKTSNSAVAAKLKQMVPSLLKEFENQIQSIRITVDTQDYKNQNDE
                     TKISKKPALNSVGIQALQQFEISLVHSPLKSAIHSLLSQYSINDRTENR"
     gene            4247..>4934
                     /locus_tag="PKZ49_10700"
     CDS             4247..>4934
                     /locus_tag="PKZ49_10700"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HND37074.1"
                     /translation="MPVKRILSAVVLTVLFTQPNDRVLAEESRCQSTTHQGVGALGRI
                     EPRSRVIRISHNAGPQGARVEQLFINESDHVKSGDTLAILSDHSKKKAEVEAATARIK
                     VLEAQRAAEQALHTYNKKEHLRHQSLQPDAAVSISQIEAKKMAYEQSLANLNRFAAEI
                     HQAQSELKVSQAELDNTLIRAPIHGTILKIHRRSGERIGDSGLLEMADLSQLDIVAEV
                     YETDLHRVRVG"
BASE COUNT         1521 a         1076 c          969 g         1368 t
ORIGIN      
        1 cttcctcaag tggaattaaa agtttagcgc tcactaaatt gtttgctaaa tcgattgcaa
       61 gtttttgggc gagatcggta ttttttgcta aaagcgcatt gagctcccgc tcagtaaata
      121 taatttcacg aggtgcatta tgttcagaat acgcttctgg ttttaaaaaa ccttcatgat
      181 cgatttcttg atcgtttgtc gaatcaggac gatctgtatt ttcatctaag aaaccaatcg
      241 atagcagctt ttcatgaaga cttttctctt ctttctcgtt aagctcaact ggcgtgaaac
      301 gggttgtaaa aacatagttt ttcagtaccc agtaagttcc tccgatcgtg atcagcattg
      361 tcagcaatac gatcataaaa atttgcgacc agttcatatg caagacctct ttttagtgat
      421 gcgctagcgg caagaataaa ttgctaatgc gcagtatagc aattcttgaa aattaccgtc
      481 gaccgtcata ccacatcgat agtttctttt tactatgcaa agtttggtaa gggtgtagta
      541 ctaatgaaag aaattgtgca aagacctcac gcgctgaata caaatggatc tttcttgacg
      601 aagtctcaat aggatgggca gttaggatcg taaacttaag acggtgagca cgtccccatt
      661 gtttcatctg ctgactaaag gaaatttctt ctgcagcata aagattcaaa ttaaaaccac
      721 ccacttcacg ataagcttgc gcgttgcata ccataagcgc accagcagcc cacttaaatg
      781 ttactgatag caccgtccat gttttcaata gcaacttagc ccaccaagtt aaattacgca
      841 tatacaatgc actacccgct gcaacatatt tcccagattg gattaactga aaaatatcat
      901 ttagtagctc tttactcatc aaacaatctg catctaaaaa aacaaaccaa tcacctctag
      961 cagcttgggc ccctgcagca cgtgcacgag cgatatgatt aatgggttca aaaacaacgt
     1021 gggcaccagc agcttttgca agactagccg tgctatcggt agaattgtta tctacgacaa
     1081 tgatctcatg cgtaaaatta aagtgttgat taaatgaaag agcttcccta atagaatgta
     1141 gacaactttc tatcaggcgt tcttcattaa aagcaggaat aattatcgag acgtgcataa
     1201 aattttgatc aataaaagac aaaagctgca gttattgtgg ctgagcttaa agctcaaggc
     1261 aagtcaagat ttgtttaacc aaaggatata aatcaaatga tatggaaacc caatgtcacg
     1321 gttgctgcag tcattgaacg agaaagaaaa ttcttgcttg ttgaagaaga tcagcaagat
     1381 ttaggcgtac aactcaatca acctgcaggt catcttgaac cctcggaatc gattattcaa
     1441 ggatgtgttc gagaagtcct agaagaaacg gcttatactt tccagccaca atggttaatt
     1501 ggtatttatc aatggcattc caccgcgact gatacaactt atttgcgatt tgcgctttca
     1561 ggtttcgtta ctgaattcga tgccaatcaa aaattagatg ttggaatttt acgtgccagt
     1621 tggtttgatc tcgaagaaat tcaacgccgc aagtcgcatc atcgtagccc cctggtgtta
     1681 agatgtattg aagattatct tgcaggaaaa cgctattcat tagatatttt tacccatcaa
     1741 acatagacta agtacatgaa gaagcagcgg attattgtcg gtatgtcagg cggggtcgac
     1801 tcctcggtag cggcattact actcaaaaaa caaggccatg aagtcatcgg tttattcatg
     1861 aaaaattggg aagacgatga tagtgatgaa tattgctcat ctcgccaaga ctttcttgat
     1921 gccgcatcag ttgcagatat catcggcatc ccactcgatg tcgtcaattt ctcagctgaa
     1981 tacaaagacc gtgtctttag caatttttta tcagaataca aagcaggtag aacaccgaat
     2041 cctgatgtgc tttgtaatgc tgagattaaa tttagcgcct ttcttgacca cgccctacaa
     2101 ttaaatgcag aagccattgc aaccggtcac tatgcacaag ttagaaaagt gggcacattg
     2161 tttcaattgc tcaaaggcga agatggacgc aaagatcaga gttatttttt atatcgctta
     2221 aatcaagatc aattagcaaa aacctgtttt ccaatcggcc acctctataa acgcgaagta
     2281 cgtcaaatcg catcagattc caaattacct aactttgcca aaaaagacag taccggaatt
     2341 tgttttattg gcgaaaggcc ttttcgcgaa tttctgaatc gttatttacc gcatgaacct
     2401 ggcgaaattc aaaccattga cggtcaggtg attggtcaac atatcggttt gatgttttat
     2461 accattgggc aacgacaagg tttgggtatc ggcggcgttc gcgacagcga tgaaagcccc
     2521 tggtttgttt gccgcaaaga tatcgaaaaa aatcgattga ttgtcgttca aggacatgat
     2581 cacccagcgc tactttctga ttctttgcaa gcaatgaatc tatcctggat tagtggcacc
     2641 gcaccgcacc aaaattgggt ctatgcggcc aaaacgcgct atcgccaaac cgatgcgccc
     2701 tgttcaattt ctcgaattga tgcgctttcc tgccagatcg attttgcgca accgcaatgg
     2761 gcggtcaccc ctgggcaatc ggttgtaatc tatgaaagca aagtttgcct gggtggtgga
     2821 ataatcaatt aatctctggg cgctgtcgca ataaatgacg ctcgctgcgc taatttttcc
     2881 gctaatcgat taagattagg gtaagaccga ggccactcaa tctgtggaaa acgaaaagat
     2941 aggtaaccga gtgcacaacc gagtgcaaca tctgccaatg aaatagactt tcctgcacac
     3001 caatctccct cccctaacat cttcgctgct tcaattaaac cccaatcgac ctttttttgc
     3061 tggcgcataa tccattcttc gctctgctgc acttcgggtc ttttacgctc cagaaaaatc
     3121 gttgctgctg catcacaaat tccatctgcg agggcttccc accgttttac ctgccaacgc
     3181 gtattacctg attccggaat caatcgaatc atcccaggct gagtttctag gctatcgatg
     3241 tattctgcga taacgcgtga gtcatacaga gaagttttct cgtctacgat aagaatggga
     3301 attttaccaa gcggattatg atgagaaacg tttgtttgag cattccaagg cacatccact
     3361 tcaaaatcgt agttgatctg cttttccgcc aaaacgatac gcactttacg cacataagga
     3421 ctggttagag aaccaattaa tttcatcatc gttatttatg cttatttcca ataatgtcag
     3481 tttactttac ttaggctacc cgtaccaagc tatactagca ggtatatttg agcatatatt
     3541 tttatctcat tatgattagc aaggattaat caacagtgaa tcatatgaac gtttgcaata
     3601 aaacgaagta actgggatta tttatattta gaaattcgat ttagctgaaa aacaacctag
     3661 ccttgccaat caatgattgg gaaaaccata acaattcatg cataagctga ccacttatct
     3721 caaagtagtt agtcgctcac gtgaccatca acgcttattt ctcaacgtca atgaaatcat
     3781 gcgactacag aaaacgctgt cgagaaagct tccgcctcaa cttgcaaaac gttgctgcat
     3841 tggtagttgt tcaaacggtg cattagtaat caaaacctcc aatagcgcag ttgcagccaa
     3901 gttaaagcaa atggtgccat cccttctgaa ggaatttgag aatcaaattc aatcaattcg
     3961 aattacagtt gatacacaag attataaaaa tcaaaacgat gaaacaaaaa tatcaaaaaa
     4021 acctgcctta aattcagtgg gaatacaagc actgcaacaa tttgaaattt cactcgtcca
     4081 ctcccccctg aagtctgcaa ttcattctct attaagtcag tactcaataa atgatcgtac
     4141 tgaaaatcgc taaagcatca atccatttga tcctgcaata aattgatcag ggaaacgatt
     4201 tgctaaaatg agcacttgtc atttgtctaa gaaaacttaa acaagcgtgc cagttaaaag
     4261 aattttgagt gctgtagtac tcaccgtttt gtttacgcag cccaatgatc gagtattggc
     4321 agaggagtcg cgttgccaat caacaactca tcaaggtgtt ggtgctttag ggcgcattga
     4381 acctcgatct cgcgtcatca gaatctcaca caatgcgggc cctcaaggcg ccagagttga
     4441 gcaactcttc attaatgaat ccgatcacgt taagtcgggt gatacactag caattttgtc
     4501 agaccacagc aagaaaaaag ctgaagttga agctgcgact gcacgcatta aagtgctgga
     4561 agcacaacgg gcagcagagc aagctttaca tacctacaat aaaaaagaac atttacgtca
     4621 ccaatccctg cagcccgatg ctgccgtgag tatctctcag atcgaggcaa aaaaaatggc
     4681 ttatgagcaa tcattagcca atctaaaccg gttcgcggcc gagattcacc aagctcaatc
     4741 cgagctaaaa gtttcacaag cggaattaga caacacgtta atccgtgcac cgattcatgg
     4801 aaccatttta aaaatccatc gcagatccgg tgaacgaatc ggtgatagcg gtttattgga
     4861 aatggctgat ttatcacagc tcgatatcgt cgcagaagtc tatgaaaccg atttacatcg
     4921 agtcagagtc ggcc
//
LOCUS       DAOQKQ010000183         4899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_9510_length_4899_cov_6.130471, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000183 DAOQKQ010000000
VERSION     DAOQKQ010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4899
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9510_length_4899_cov_6.130471"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="PKZ49_10705"
     CDS             <1..577
                     /locus_tag="PKZ49_10705"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HND37075.1"
                     /translation="HIGKLIRIRKDGSVPTDNPFTKLSVAKPEIWSYGHRHIQGAAIH
                     PGTGVLWIHEHGPRGGDEINIPLPGKNYGWPLASYGMHYSMEPIKDEHAEQGFEEPIY
                     YWNPSIAPSGMMFYRGNLFPEWKGNLFIGSLVGRHLVRLVVDSNQVISEEKLLMNQAR
                     IRDVAEGPDGAIYLLTDDTNGLLLKLSRPTP"
     gene            complement(613..1362)
                     /locus_tag="PKZ49_10710"
     CDS             complement(613..1362)
                     /locus_tag="PKZ49_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HND37076.1"
                     /translation="MNTGREEFIDLSFNTSLREFLLIKEFQGILLSDLEKIIHTCHQQ
                     CYKAGEEIVRYQDHTNSVFFILKGIIRIHYYSLSGDEVILCDLPEGEMFGELTAIDGL
                     ARSATAVALKDSLLASLPSSAFLELVHTYPEFCTAILKRLVGQVRRLTERVFDFSTLA
                     VRNRIHAELLRLTHKNKVSENLAIISPAPTHEELANYVSTRREEVSREISVLARQGII
                     ECRGRAWHILDVAKLAHMVKDVRGSFDTEKV"
     gene            complement(1476..1637)
                     /locus_tag="PKZ49_10715"
     CDS             complement(1476..1637)
                     /locus_tag="PKZ49_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37077.1"
                     /translation="MNNDDLEVNSQNFEVMNADINEERTPMPRSGSWHWVPGHVEGGA
                     WIAGYWEQD"
     gene            complement(1703..2122)
                     /locus_tag="PKZ49_10720"
     CDS             complement(1703..2122)
                     /locus_tag="PKZ49_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37078.1"
                     /translation="MKAILSISISLLILTGCHTFGIHKLGGESGGKPNCFNRSTDNEC
                     SAPNLENVVLTSEVETALKKLGIPDVSFIAVVSKDGKITFAKSAATEVKRFQLPSTFT
                     IESIQSYSLIKFKGSTCKSWIPGHVEGGAWISGYCDD"
     gene            complement(2132..>4899)
                     /locus_tag="PKZ49_10725"
     CDS             complement(2132..>4899)
                     /locus_tag="PKZ49_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HND37079.1"
                     /translation="ALETLDQNTLSAQLLTYRAGLYLTLGRVAEARHDIAQTLQLQPN
                     DSEAYALQAIITVVQNDKVAALQLAEQAVNADRTSPTARLALSYAYQAHFQIEEALAS
                     VEEALRLDGKNALVWARLSELQMSTGYLDRALESAQQAVSLNPNLGKTQTVLGFAHLL
                     QIDTTQAKATFAKAIQLDQADPMPRLGLGLALIREGQLEAGRIELEIAASLDPANSLV
                     RSYLGKAYFEEKRYGLAETQFDLAKARDPNDPTPWFYDAIQKQTQNRPVEALRDIQKS
                     IELNNNRAVYRSKLLLDKDEAARGSSLARIYDNLGFEKRAIMETAKSLSHDPGNHSAH
                     RFLADAYVNIPRHEIARVSELLQAQLLQPINVNPVQPRLAVADLNIITGTGPAALGFN
                     EFAPLTERNKPQVVASGLFGSNSTLGNEVVASAVYDKASISVGQFHYDTKGFRQNNDQ
                     THNVYNAFMQYALTPKLNVQAELRTRGTEHGDLIMDFGRQGDPTDPPNFTRNRRALNE
                     DIARVGARYAISPKQDVLFSGQYVNRDENLKFFSSDDITSKIKDEGYQTEAQHLLRGT
                     FFNLVSGLGAYRTDVNNIRHVDFTRRDGEACKSSRLNNDFCDPDLPGFTRERTNGYVY
                     ANVSHLKNMNWTFGLSYDAYKEFRDFDVNSLNPKFGLQANITNFLRLRLAWFETVKSA
                     LIANQTLEPTQIAGFNQFFDDVNGTKSQRKGIGLDANFTKDLYSGVEYSERDLEAPQF
                     NGPGRTTQRIPNSIYTLSSIDKQHEKLFRTYLYWLPISNWVVRSEFQYEHFERDQAKT
                     PPLEVNRINTLSTPINISYFDPSGLFAGMTTTFVLQDIERSAANLPEDQRNSAKLNAG
                     MDSFFLWDTVLGFRMPNRRGVLSLEGRNLLDQDFLYRSINFQQSEAIASRFIPSRTIF
                     LRLTLNF"
BASE COUNT         1377 a         1085 c          947 g         1490 t
ORIGIN      
        1 ccatattggc aaactcatcc gtatccgcaa agatggcagc gtgcctactg ataatccttt
       61 tactaagctc tcagtggcca aacccgaaat ttggtcctac ggtcaccgcc atattcaagg
      121 cgcagccatt caccccggaa cgggggtatt atggattcac gaacatggtc cacgaggcgg
      181 tgacgaaatc aatattccac tcccaggtaa aaattacggc tggcctttag caagctacgg
      241 tatgcattat tctatggagc cgatcaaaga tgaacatgct gaacagggct tcgaagagcc
      301 gatttactat tggaacccgt ccattgcccc ttctgggatg atgttttacc gtggtaattt
      361 gttcccagag tggaaaggta atttattcat tggctctctg gtaggacggc atctggtccg
      421 tttagtggtt gacagcaacc aggtgatcag tgaagaaaaa ttgctcatga accaagctcg
      481 tattcgcgat gtcgcagaag gacctgatgg tgctatttat ctactcactg atgatacaaa
      541 tggcttattg cttaaactat cgcgccccac accctaaaaa cacaaatcgt ggaacagttc
      601 tctcatagtg agtcaaactt tctcagtatc gaatgaacca cgaacatctt tgaccatatg
      661 tgctaactta gctacatcca agatatgcca tgcccgtcct ctgcactcga tgataccttg
      721 acgtgcaagg acagatatct cgcgtgaaac ttcctcacgc cgagtactta cataattagc
      781 taattcctca tgggttggtg ccggcgaaat gatcgccaag ttttccgaca ccttgttttt
      841 atgggttaag cgcagtaatt ctgcatgtat gcggttccga acggccaagg tactaaaatc
      901 aaacacccgc tcagtcaagc gcctcacctg accaaccaat cgctttaaga tagccgtaca
      961 aaactcagga taagtatgga ctagctcgag aaaagcgctg ctgggcaaag aagccagcag
     1021 cgaatctttt aatgcaacgg ccgtcgcaga tcgagctaat ccatcgatag cggttaattc
     1081 accaaacatc tctccctcgg ggagatcaca taaaataacc tcatccccgg acagagaata
     1141 ataatggatt cgaattattc cctttaagat aaagaaaaca ctgttagtgt gatcttggta
     1201 tctaacaatt tcctctccgg ctttgtaaca ttgttgatgg caagtatgaa ttattttttc
     1261 taaatcactg agtaaaatac cctggaactc ctttattagg agaaattcac gcaagctcgt
     1321 gttaaaactg aggtctataa actcttccct tcctgtattc atttttcttt tttaagcaaa
     1381 aacatttggc tcatagcttt tatggataag cttatggtcc gtataactat ctatacagat
     1441 cataagctta ttaatgttct aattttattt aaaaattaat cttgctccca ataaccagca
     1501 atccacgccc ccccttcaac atgtccgggc acccaatgcc aactcccact tcttggcatg
     1561 ggtgttcgtt cttcatttat atctgcattc ataacttcga agttttgtga gtttacttct
     1621 aaatcatcat tattcatttt ttccctcata tttgttaaaa aaaagaaata gcagattaca
     1681 taaaaaaata atttaaatta gattaatcat cacaataacc acttatccat gcaccgcctt
     1741 caacatgccc aggtatccaa gatttacaag ttgatccttt aaatttaatt aaagaataag
     1801 attgaatact ttctatagta aatgtacttg gaagttggaa tcttttaact tcagttgcag
     1861 cagatttagc aaatgttatt tttccatctt tactaacaac tgctataaat gatacatcag
     1921 ggatacctaa tttttttaaa gctgtttcaa cttcacttgt taatactaca ttttctaaat
     1981 ttggcgcact acattcgtta tctgtagatc tattaaagca gtttggtttt cccccactct
     2041 ccccccctaa tttatgaata ccaaatgtat gacaccctgt taagattaat aaactaatgg
     2101 atatactcaa tattgctttc atttttcctc cttaaaagtt aagtgttagt cgtaaaaaaa
     2161 tcgttctgct agggataaag cggctagcaa ttgcttcaga ctgctgaaaa tttatacttc
     2221 tatataaaaa atcttgatct aataaatttc ttccttctaa actcagaaca cctctccgat
     2281 taggcattct aaaacccaat actgtatccc ataaaaagaa gctatccata ccagcattca
     2341 acttagctga attccgctga tcttcaggca aatttgcagc tgatcgttca atgtcttgta
     2401 gtacaaacgt tgttgtcatt cccgcaaaca agccagaagg atcgaagtaa cttatgttga
     2461 ttggagtgct caatgtattt atccggttta cctctaaagg tggtgttttt gcttgatcac
     2521 gctcaaaatg ctcatattga aactcacttc taactaccca attcgaaatg ggaagccaat
     2581 acaaataagt acggaacagc ttctcatgct gtttatcaat actacttaat gtatagatag
     2641 aatttggtat tctctgagta gtacgccctg gcccattaaa ttgcggcgcc tcaagatcac
     2701 gctcagaata ttccacaccg ctatataaat ctttcgtaaa attcgcatcc agcccaattc
     2761 cttttcgttg tgatttcgtg ccattcacat cgtcaaaaaa ttgattaaaa ccggcgattt
     2821 gagttggttc taacgtttga ttagcaatca atgctgattt aaccgtttca aaccaagcaa
     2881 gcctcaatcg tagaaaattg gttatgttag cttgtaaccc gaatttaggg tttaaactat
     2941 taacatcaaa atctctaaat tctttgtacg catcataact aagcccgaaa gtccagttca
     3001 tatttttgag atgactaaca tttgcataga cataaccgtt tgttctttct cgagtaaatc
     3061 ctggaagatc aggatcacag aaatcattgt ttagcctact tgacttacac gcttcaccat
     3121 ctctcctggt aaaatcaaca tgccgaatat tgttaacatc ggtacgataa gcgccaaggc
     3181 cacttacaag attaaaaaag gtaccccgca ataaatgttg tgcttcagtt tgataaccct
     3241 catctttaat cttgctggtt atatcatcac tactaaaaaa ctttaaattt tcatcacggt
     3301 taacgtattg cccagaaaat agtacatctt gcttaggaga tatcgcatac ctagcaccaa
     3361 cccgagcaat atcttcattc aatgctcttc tgttgcgagt aaaattaggt ggatctgtag
     3421 gatcaccttg tcttccaaaa tccatgatta aatccccatg ttcagttccg cgtgttctga
     3481 gttcagcttg cacattcaat ttaggggtca aagcatactg catgaatgca ttgtaaacat
     3541 tatgggtttg atcattattt tgccggaacc ccttggtatc atagtggaac tgccccacgc
     3601 taatcgatgc tttgtcataa actgcagaag ctacgacttc gttgcctagc gtactgtttg
     3661 aaccaaacaa ccctgatgca accacttggg gcttattacg ttccgtcaat ggcgcaaatt
     3721 cattaaaccc taacgctgca gggcctgttc cggtaataat gtttaaatct gcgaccgcta
     3781 atcttggttg cactgggtta acattaatcg gttgtagcaa ttgtgcttgc agtaactcac
     3841 tgacgcgtgc aatctcatgg cgcggtatat ttacataagc atcggccaag aatcgatgcg
     3901 cggaatgatt tcctggatca tggcttaatg atttcgctgt ttccatgatg gcacgtttct
     3961 caaaccctaa attgtcgtag attctggcaa ggctagagcc tctggcggct tcgtctttgt
     4021 ccaggagtaa tttggagcga taaacagcac gattattatt gagctcgatg gatttttgaa
     4081 tgtcgcgtaa tgcttctacc ggtcggttct gcgtttgctt ctgaattgca tcataaaacc
     4141 acggggttgg gtcattagga tcgcgtgctt tggccagatc gaattgggtc tctgcaagtc
     4201 catagcgctt ttcttcaaag taggctttac ctaagtagct gcgtactagg gaattggcag
     4261 ggtctaaact tgcagcaatt tcaagctcga tgcgccctgc ttcaagttgg ccttcacgaa
     4321 taagtgcaag ccctaaccca agccttggca tgggatcggc ttgatcaagc tgaatggctt
     4381 tagcgaatgt tgctttcgct tgggtggtat cgatttgtaa tagatgagca aatcctaata
     4441 cggtttgggt tttacctaaa ttaggattaa gacttaccgc ttgttgtgcg gattctaatg
     4501 cgcggtcgag ataaccggtg gacatttgta attctgatag ccgtgcccaa accagtgcat
     4561 tcttgccatc gaggcgcagc gcttcttcta cactggcgag tgcttcttca atttgaaaat
     4621 gggcttgata ggcataagaa agtgctaacc gtgcggtcgg tgaagtacga tcagcattaa
     4681 ccgcttgttc agcgagttgc aatgcggcta ctttatcgtt ttgcaccacg gtgatgatgg
     4741 cttgtaaggc ataagcttcg ctatcattgg gttgcagttg tagcgtttgg gcgatatcgt
     4801 gtcgcgcttc tgccacgcgt cccagtgtta aatagagtcc ggcacggtaa gtgaggagct
     4861 gcgcgctaag ggtattttga tctaatgttt cgagtgcag
//
LOCUS       DAOQKQ010000184         4872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_9592_length_4872_cov_6.437202, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000184 DAOQKQ010000000
VERSION     DAOQKQ010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4872
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9592_length_4872_cov_6.437202"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1241
                     /locus_tag="PKZ49_10730"
     CDS             <1..1241
                     /locus_tag="PKZ49_10730"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HND37080.1"
                     /translation="TLLHALDTGAIDFLISEDRGLHARARQHASHLSRRVLFIADAFA
                     LLTGTYEPVEVPIRYVDEVGANEIPLTDNIFDSLREGYDGFDEWWRTKCVNKHRRCWV
                     VYDEGLAGLIVRKDESKEDTDATLPGKKILKVCTFKVRPEKRGIKLGELLLKQALWFA
                     QSNKYDIVYLTTQAQQEALIDLLEYYGFCHTKTLNDKELIFEKFLSRDRVEIDDDEDL
                     YEIAKRYYPRFYTGSRVQAYVIPIKESYHDMLFPELRNNIQGDLLTPIGHGARPTRPG
                     NTIRKVYLCRAPANLTSPGSLLFFYKGKSDLPPSQAITTIGIFEDMQEATSLMELMQL
                     TGGRSVYREDELKKWQATTEKPVKVINFLLAGYFSPPLVLGALKTEQIFGSRPPQSIA
                     GLTRTQLDKLFAHLDLGFQT"
     gene            1241..1819
                     /locus_tag="PKZ49_10735"
     CDS             1241..1819
                     /locus_tag="PKZ49_10735"
                     /inference="COORDINATES: protein motif:HMM:NF024605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HND37081.1"
                     /translation="MNAKRIIALAGLSGAGKTTLIRQVVDESKVVHLSASELIKTQIK
                     VENVVDKTSEALRTGNIANNQDRLITAFTAIAPSITEHIILGCHTIIDTPTGIQEIPI
                     EVFKTIKITDFLFLSVDPMELYQRREKDMLRSRPIRTIEELESQQKIGLELAHQIAEA
                     LCIPFFDIGAAPEEQLLSILQSLPLLSKRTPR"
     gene            complement(1991..2155)
                     /locus_tag="PKZ49_10740"
     CDS             complement(1991..2155)
                     /locus_tag="PKZ49_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37082.1"
                     /translation="MSLIVVGNALGVKEGRGAYDKTIVFFVCFGRNGKAFTKIGPDTS
                     SYIASHYPCA"
     gene            complement(2458..3621)
                     /locus_tag="PKZ49_10745"
     CDS             complement(2458..3621)
                     /locus_tag="PKZ49_10745"
                     /inference="COORDINATES: protein motif:HMM:NF013788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C13 family peptidase"
                     /protein_id="HND37083.1"
                     /translation="MGYLGIELIGTLLIGFLLTKFPGNKDLLLKFLILVYCISPFLIL
                     IFSLVISKLPDEYYFLGYLAFLAWKYSAYYYIVLQIQDQNKVMGLMITCGLFITAYPL
                     IIHSHTLTFWYEDYYQTSEQPIYTGDVLNYVNQEQLYYDQYQLLHNSLDLIHPGVLGK
                     SEIFFVGFGSDATQDVFMKEAKHIQNVSDAYLGTNDRSMVMINNIATMDTVPLATSTN
                     LALSLAHISRKMNLEEDVLFLYLTSHGSGNHELSVNMWLLDLNDIRPEDIKAYLDEAG
                     IRWRVILISACYSGGFIQKLQNENSLIFTAAAADKTSFGCVNENEFTYFGETVFDQIK
                     REPYSLETSFKMAIEKIKQRELSENLPHSEPQLFIGNMMLDKLKQLEQVARFP"
     gene            4023..4862
                     /gene="nadC"
                     /locus_tag="PKZ49_10750"
     CDS             4023..4862
                     /gene="nadC"
                     /locus_tag="PKZ49_10750"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HND37084.1"
                     /translation="MQIDEIRKNVAIALAEDVGSGDLTAQLISNDANIAAQVISREAA
                     ILCGIAWFEACFNQLSSDIAIHWLVKEGDILQPNQTLCKIEGNARTILTAERSALNFL
                     QFLSAVATQTHQYVKAVSGTHALIVDTRKTIPGLRYAEKYAVRCGGGANHRIGLYDGI
                     LIKENHIIASGSIAQTLHKARTIAPTETMIEIEVETLDELHQALHAGATMILLDNFEL
                     ETLRNAVILNQQYTQPAILEASGNITLTNVRSIAETGIDRISIGSLTKNIKAIDLSMR
                     FLH"
BASE COUNT         1563 a          958 c          934 g         1417 t
ORIGIN      
        1 caacattact gcacgcgctg gatacaggag ctattgactt tctcatctcg gaggatcgtg
       61 ggctacatgc gcgagcacgg caacacgctt cgcatttatc aagacgtgta ttgttcatcg
      121 ctgatgcatt cgccctatta accgggacct atgaaccggt cgaagttcca attcgttatg
      181 tcgatgaggt tggtgcgaac gaaattccat taaccgataa tatttttgac agtctgcgtg
      241 aaggttatga tggatttgac gaatggtggc gaacaaaatg cgttaataaa catcgacgct
      301 gctgggttgt ttatgatgaa ggtctagcgg gcttaattgt tagaaaagat gagtcaaaag
      361 aggatactga tgccactttg cctgggaaaa agattctcaa ggtttgtacg ttcaaagtca
      421 ggcccgagaa acgcggaatt aagcttggag aattgctact caagcaggcg ctttggtttg
      481 cacagtcaaa taaatatgac attgtttatc ttacaacgca ggcacagcaa gaagcactga
      541 ttgatttact cgaatattat ggtttctgtc atacaaaaac acttaacgat aaggaattga
      601 ttttcgaaaa attcctttca cgtgaccggg ttgagattga tgacgatgaa gatttatatg
      661 aaatagcaaa gcgttattat ccacgattct acactggttc ccgagtgcaa gcgtatgtta
      721 ttcctattaa agaatcttac catgacatgc tttttccaga gctgagaaat aacattcaag
      781 gcgatctatt gacaccaata ggacatggtg caaggccgac tcgtcccgga aacacaatac
      841 gaaaagttta tctctgtcgt gccccggcca atcttacaag tccgggatct ctcttgttct
      901 tttataaggg taaatcagat ttacctccgt cgcaggcgat aacaacaatt ggtatttttg
      961 aagatatgca agaagcaacg tcattaatgg aacttatgca actgacagga gggcgatctg
     1021 tatatcgaga ggatgaactg aagaaatggc aggcaacaac tgaaaaacca gttaaagtca
     1081 tcaatttcct tcttgcaggt tatttttcac caccccttgt tctgggtgct ctaaaaacag
     1141 aacagatttt tggaagtcgg ccacctcagt ccattgctgg tttgacaaga acacaattgg
     1201 ataaattgtt tgcacatcta gatttaggtt ttcagacata atgaacgcaa agagaattat
     1261 cgcattggct gggttatctg gtgcaggtaa aacaacattg atacgccaag ttgttgatga
     1321 atcaaaggtt gttcacctct cagcaagtga gcttatcaaa acccaaatca aggttgagaa
     1381 tgtagttgat aaaacatcgg aggcactacg caccggtaat attgctaaca atcaagatcg
     1441 gcttatcaca gcatttacag caattgcacc gagcataaca gagcatataa ttttgggctg
     1501 tcatacgatc attgatacgc caacgggaat tcaagagatt ccgatagaag ttttcaagac
     1561 tataaagata acagattttc tttttctcag tgttgatcca atggaactct atcagaggcg
     1621 agaaaaagat atgctgcgct cgcgaccaat tcggacaata gaagagctgg aatcacaaca
     1681 gaaaattggt cttgaattag ctcaccaaat tgcagaagct ctttgtatcc cgttttttga
     1741 tattggtgca gctcctgagg agcagctttt atctattctg caatctttac ctttactctc
     1801 aaaaagaact ccgcgatgag atgaatattt cgcaaaactc cagaaagatt cgatactgtt
     1861 atatacctct tgctactgga aaactagtta ctgccatgac gaaccctgaa gggactatca
     1921 gagctgatag cgaccacaat ccgttattag ttgcgccatc catcagaata aatgatagta
     1981 ctctggcgcg tcatgcgcat ggataatggc ttgcaatgta gcttgaagta tctgggccaa
     2041 tcttcgtaaa tgctttccca ttgcgcccaa aacacacaaa aaaaacaatc gttttgtcat
     2101 aagcacctcg accttcttta acccctaatg cgttgcctac cacaataaga ctcaacgact
     2161 tacacgatgc ctcaaaaagg tgatttaagt tctcaactca ctctggtagc cagatcatcg
     2221 aacgtcattg caagaaagta cttggagcac agattgaagg tgctcacaat tatttatctc
     2281 atcttacaac tctttcacaa ggtcattggc gagaaatact ccaactccta cagagaaatc
     2341 ttttggatca aaacactgct gaacaagcga tctccggtgc aaattcagct ttctcattat
     2401 tagaaatact agcaaccgag aaataattca tcacttttct aaactatcta aaagttatca
     2461 cggaaatctt gccacttgct ctaattgctt tagcttatct agcatcatat ttcctataaa
     2521 tagctgaggt tcagaatgcg gtaaattttc agataattca cgttgcttaa ttttctcgat
     2581 tgccatttta aagcttgttt caagggaata aggttctctt ttaatttggt caaatacagt
     2641 ttctccaaaa taagtaaatt cattttcgtt gacgcaacca aacgatgttt tatctgcagc
     2701 tgcagccgta aaaatcaaac tgttttcatt ctggagtttt tgaataaacc cacctgaata
     2761 gcaagcagaa attagaatga cacgccaacg gattccagct tcatctaagt atgctttaat
     2821 atcttcagga cgaatatcat taagatccag caaccacata ttgacggaaa gttcatgatt
     2881 accactacca tgactggtta aatataaaaa cagtacatct tcctctagat tcatttttcg
     2941 actgatatgt gcgagtgaga gtgctaaatt tgttgatgtt gctaagggaa ccgtatccat
     3001 cgttgctatg ttgttaatca ttaccattga tcggtcattt gtaccaagat acgcatcact
     3061 tacattctgt atatgcttgg cttctttcat aaaaacatct tgagttgcat ctgaaccaaa
     3121 tccgacaaaa aatatttctg acttccctaa tactccgggg tgaattaaat ccaatgaatt
     3181 atgtaaaagt tgatattgat cgtaataaag ctgttcctga tttacgtaat taagtacatc
     3241 tcctgtgtaa attggctgtt cactcgtttg ataataatct tcgtaccaaa aagtcaaagt
     3301 atgcgaatga atgattaacg gataagctgt tatgaaaagt ccgcaggtta tcatcagtcc
     3361 catgacttta ttctgatctt gtatttgtaa tacgatgtaa taataggcac tatacttcca
     3421 tgccagaaaa gcgagatacc caagaaaata atattcgtca ggcagttttg atatgactaa
     3481 ggaaaaaatc agaattaaaa aaggtgaaat gcagtaaacc aatatcaaaa actttaataa
     3541 taagtctttg ttcccaggaa attttgtcaa taaaaagcca atcaataggg tgccaattaa
     3601 ttcaatcccg agataaccca aaccatacca ctcgaatact gggcgatcag ttatccaata
     3661 ggtagcaata actgcggtta gtccatagca gatgagcaat aaaaaaagtt ggtcatgtga
     3721 aaccgttagg tatcttagtt gatcatttcg aaatactaat agtctgaggc cacaatttaa
     3781 attatataaa agtgaatgaa attctttgat taatgtggat aattttgatt ccttttgtaa
     3841 gcttgtggac caattcattc tcatctccaa cttttctagt agatagcgtt atgaacggca
     3901 ataataaatt gggttggagg ctttaacttc atcctagtag ttcataaact tcatatgcaa
     3961 tttatcgcag aaatttgcat tactatggtt taattaagca tcaattaaca tgagttttca
     4021 gaatgcaaat cgatgaaatt cgaaaaaatg ttgcaatcgc gcttgctgaa gatgttggaa
     4081 gtggcgattt aactgctcaa cttatttcaa atgacgcaaa tatcgctgca caagtcatta
     4141 gtagggaagc agcaatatta tgtggtattg cttggtttga ggcgtgtttc aatcagctat
     4201 catcagatat tgcaattcat tggcttgtca aggaaggaga cattcttcaa cccaatcaaa
     4261 ctttatgcaa aatagaggga aatgctcgca caatattaac agcggaacgc tcggcactga
     4321 attttctgca atttctctct gccgttgcga ctcagactca tcaatacgtt aaggcagtat
     4381 caggcacaca tgcgttaatt gttgatactc gtaaaacaat tcccggtctt cgatatgctg
     4441 aaaaatatgc agtaagatgc ggtggcggag ccaaccaccg gataggttta tacgatggca
     4501 ttttaattaa agaaaatcac attattgctt caggtagcat agcccaaacg ctacataagg
     4561 ccagaacgat tgctcccact gaaaccatga tagaaattga agtagaaaca ctcgatgaat
     4621 tgcaccaggc gctccacgct ggcgcaacaa tgattctatt agataatttt gaactcgaaa
     4681 cgctgcgtaa cgcagtgata ttaaatcaac aatataccca accagccatc ttggaagcat
     4741 caggtaatat tacattaaca aacgtgcgct caattgctga aacaggtatc gacagaattt
     4801 ctattggtag tttaacaaaa aatatcaaag cgattgattt gtcgatgcga tttctgcact
     4861 aactactaat ca
//
LOCUS       DAOQKQ010000185         4771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_9926_length_4771_cov_5.849237, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000185 DAOQKQ010000000
VERSION     DAOQKQ010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9926_length_4771_cov_5.849237"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="PKZ49_10755"
     CDS             <1..810
                     /locus_tag="PKZ49_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HND37085.1"
                     /translation="TWRKWSDYITLQPYYMGQKQTADPNGFTATNRLDREIHMPGFRA
                     YGRLGIPGQTYILDFDASYNQQLGFSGPLKQDAHGYTIEIGRTFDHPWKPRISTFYGY
                     ASGDRDPNDGEDNRFDRFFGFARPWSADHYVIYENLKAPKIRFEFQPTPILGFEMGYG
                     AYWLASGTDRMFDILDGNISNTIKDPGFNRDRTGKSGDFGGHALEGRIRFQPTPRIQT
                     ILGYTHFTAGEFVKNRIAASPSCADGHAHPCVDRRSGNTDFLYFEVLISLL"
     gene            857..1855
                     /locus_tag="PKZ49_10760"
     CDS             857..1855
                     /locus_tag="PKZ49_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HND37086.1"
                     /translation="MKLQTWFTVLFIITSLSIIGPARADKTLLNVSYDPTREFFQEFN
                     AAFSKHWLVNGGEKITIQQSHGGSGKQARSVIDGLAADVVTLALAGDIDAIAEHGKLL
                     PENWQTRLPRNSSPYTSTIVFLVRKGNPKGIKDWHDLANGGVAVITPNPKTSGGARWN
                     YLAAWEFGKHYFGETRVQEFVSDIYKNVPVLDSGARGSTTTFVERGIGDVLIAWENEA
                     YLALKEYGNDKFEIIVPSVSVLAEPPVAIIDKVVDKKGTRVIAEAYLNYLYSEEAQHI
                     AAKHYFRPSDETIAAQYAQQFTKVKLFKISDTFGSWKNIHQIHFSDGGIFDQIYLK"
     gene            1908..2741
                     /gene="cysT"
                     /locus_tag="PKZ49_10765"
     CDS             1908..2741
                     /gene="cysT"
                     /locus_tag="PKZ49_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HND37087.1"
                     /translation="MSQFKQHSVLPGFNLAIGFTLLYLSLVVLIPLSAAFIRATELTW
                     SEFWTVVTTPRVMASYRLTFGASFIAALINAIFGLLVAWILVRYQFTGKKIIDALIDL
                     PFALPTAVAGITLTALYAGNGWFGQFLEPLGIKVAYTPIGVFVALTFIGLPFVVRTVQ
                     PVLEDIETELEEAAAMLGASRLQTFVRIIFPTILPALMTGFALAFARAIGEYGSVIFI
                     AGNMPMVSEITPLLIITKLEQYDYAGATATAVVMLVTSFVLLLIINLLQWWGRRRNAL
                     I"
     gene            2748..3626
                     /gene="cysW"
                     /locus_tag="PKZ49_10770"
     CDS             2748..3626
                     /gene="cysW"
                     /locus_tag="PKZ49_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HND37088.1"
                     /translation="MATITVSSSHSPSRTRITQEPRWVRYTLITIALTFLALFLFVPL
                     ISVFYEALKKGVAVYIAAISDPDALAAIKLTLTATAIAVPLNLIFGVAAAWAIAKFEF
                     KGKNVLITLIDLPFSVSPVIAGLIFVLVFGMQGWLGPWLAEHDIRIIFAVPGIVLATL
                     FITVPFIARELIPLMQAQGTEEELAAVTLGANGWQLFWRITLPNIKWGLLYGVILCNA
                     RAMGEFGAVSVVSGHIRGSTNTLPLHVEILYNEYNFAAAFAVASLLALLALITLVVKT
                     FIESKVSQQKGTHHEH"
     gene            3616..4689
                     /locus_tag="PKZ49_10775"
     CDS             3616..4689
                     /locus_tag="PKZ49_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HND37089.1"
                     /translation="MSIEIRNLHKQFGSFTALNNVSLDIQPGELLALLGPSGSGKTTL
                     LRVIAGLETVDSGQVLFNNEDATHQHIHERQVGFVFQHYALFRNMTIFENVAFGLRVR
                     PKSVRPDNEEINKRVTDLLKLVQLDWLGDRYPHQLSGGQRQRIALARALAVEPKVLLL
                     DEPFGALDAKVRKELRTWLRRLHDDMHITSVFVTHDQEEALEVSDRVVVMNEGRIEQI
                     ATPDEVYEKPANPFVYEFLGHVNLFHGRVHKGRAWIGDIEIDAPDHAEAEDLAAVAYV
                     RPHDIEVDREPNGESALAAQIVHILSVGPIVRLELAREENQGKNLIQVEISKERFRGL
                     QLEKGDRVFIKPRRLDLFPHLTH"
BASE COUNT         1263 a         1124 c         1044 g         1340 t
ORIGIN      
        1 acctggcgca aatggtctga ttacatcacg ttgcaaccct actatatggg tcaaaagcaa
       61 acagcggatc ctaatggctt tactgcaacc aatcgcttag accgcgaaat ccatatgcca
      121 ggattccgcg cttatggccg actagggatt ccaggacaaa cctatatctt agattttgat
      181 gcaagctaca accagcaact cggtttcagt ggccctttaa aacaagatgc gcatggttat
      241 accatcgaaa ttggccgtac cttcgatcat ccttggaaac cacggatcag caccttctat
      301 ggctatgcat cgggcgatcg tgaccctaat gatggtgaag ataaccgctt tgaccgcttc
      361 tttggatttg cgcgcccttg gtcggctgat cattatgtca tctacgaaaa cttgaaagca
      421 cctaaaatcc gctttgaatt tcaacctacc ccaatactcg ggtttgaaat ggggtatggt
      481 gcttactggt tagcaagcgg caccgatcgt atgttcgata ttctcgatgg caatatcagc
      541 aatacgatca aagatccagg atttaatcgg gatcgtaccg gaaaaagtgg ggattttggc
      601 ggacatgcgc ttgaaggccg tatccgcttt cagcctacac caagaattca aacgatattg
      661 ggttataccc atttcacagc aggtgaattc gtaaaaaatc ggattgcagc tagcccatct
      721 tgcgccgatg gtcatgcaca cccttgcgtc gaccgtcgct caggcaatac cgatttctta
      781 tattttgaag tacttattag cctactttaa ttctcaacaa aattttaatc atcaatttga
      841 atcgaaagga aaaactatga aattacaaac ttggttcacc gtattgttca tcattacaag
      901 cttatccatc attgggcccg ctcgtgctga taaaacccta ctgaatgttt cttatgatcc
      961 caccagagag ttctttcaag aatttaatgc cgcgtttagt aaacattggt tagtaaacgg
     1021 tggtgaaaaa ataactattc aacaatcgca tggcggctcc ggtaaacaag cacgttcagt
     1081 aatcgatggt ttagcggctg acgttgtaac actcgcgtta gccggcgata tcgatgccat
     1141 tgccgaacat ggaaaactat tacccgaaaa ttggcagact cgtctaccta gaaacagctc
     1201 accctacaca tccaccatcg ttttcttagt gcgtaaaggt aaccccaaag gaatcaaaga
     1261 ttggcacgat ttggcgaatg gtggtgttgc agtcattaca cccaatccca aaacatccgg
     1321 aggcgcacgt tggaattact tagctgcttg ggagtttggt aaacattact tcggtgaaac
     1381 gcgcgtacaa gaatttgtat ccgatattta taaaaatgtc ccagtactcg attcaggtgc
     1441 acgcggttca accactacat ttgtagaacg gggtattggt gatgtcctca tcgcgtggga
     1501 aaatgaagcc tatctggcac tcaaggagta tggcaatgat aaatttgaga ttatcgttcc
     1561 ttccgtcagt gttttagcag agcctccagt tgcaatcatc gacaaagtgg tcgacaaaaa
     1621 aggtactcgg gtaatcgctg aagcctatct caattatctt tactcggaag aagcccaaca
     1681 catcgcagct aagcattatt ttcgtcccag cgacgaaact atcgcagctc aatacgcgca
     1741 gcaattcact aaagtgaaat tattcaaaat aagcgatacc ttcggtagct ggaaaaatat
     1801 tcatcaaatt catttcagtg atggcggcat cttcgatcaa atttatctga aatagcctat
     1861 tgccagatga ttctagattt atccaataat cttcggatag ttcaaaaatg agccaattta
     1921 aacaacatag cgtattacct ggattcaatc tggcaatcgg cttcactctg ctttatctga
     1981 gcttagttgt actcattcct ctttctgctg catttattcg tgctacagaa ctaacctggt
     2041 ctgaattctg gacggttgta accacccctc gcgtgatggc ttcatatcga ctgacctttg
     2101 gggcttcctt tatcgctgcg ctaatcaatg ccatattcgg tttgctcgtt gcatggattc
     2161 tcgtgcgcta tcagttcacc ggtaaaaaaa tcattgatgc cctgatcgat cttccgtttg
     2221 cgctaccaac cgccgttgca ggcataacgc taacagcgct ttatgcaggc aatggttggt
     2281 ttggtcaatt tctagagcca cttggcatca aagtagccta cacccctatc ggagttttcg
     2341 ttgcgcttac cttcattgga ctcccctttg ttgttcgcac cgtacaaccc gtattggaag
     2401 atatcgagac agagcttgag gaagctgcag caatgttagg cgcaagtcgg ctacaaacgt
     2461 tcgttcggat tattttccca acaatcctac cggcattgat gaccggattt gccttagctt
     2521 ttgctcgcgc catcggtgaa tacggttcag tcattttcat cgctggaaac atgcccatgg
     2581 tttcggaaat caccccgcta ctaatcatta ctaaacttga gcaatatgat tacgcgggtg
     2641 ctaccgcaac tgctgtcgtg atgctcgtta cttcttttgt gctccttctt attattaatc
     2701 tactgcaatg gtggggacgc cgtcgcaatg cactgatata aatggatatg gctactataa
     2761 ctgttagttc ctctcattcc cctagtcgca ctcgaattac tcaagaaccg cgatgggttc
     2821 gctacacact gattacgatt gcgctcacat ttctcgcact atttctattt gtgccgctca
     2881 tttccgtttt ttatgaagcc cttaaaaagg gggttgcggt ttatattgca gcgatcagcg
     2941 atcccgatgc actcgctgcg atcaaactta cgttaactgc aaccgccatt gcagtgccac
     3001 ttaatctcat ttttggcgtt gctgccgcct gggccattgc taaatttgaa ttcaaaggta
     3061 agaacgttct gatcaccttg atcgatttac cattctcagt ttcaccggtt attgctggcc
     3121 taatctttgt gctcgttttt ggaatgcaag ggtggttagg cccttggtta gccgaacacg
     3181 atatcagaat catcttcgca gttcccggca ttgtactcgc tacgctcttc attaccgttc
     3241 ccttcattgc acgcgaactc attcccctca tgcaagccca agggaccgag gaagaactcg
     3301 ccgctgtgac cctaggcgcc aatggttggc agttattttg gcgtattacg ctacccaata
     3361 ttaaatgggg tctgttatat ggcgtcattc tctgtaatgc ccgtgcaatg ggtgaatttg
     3421 gtgcagtttc tgtggtatcc gggcatattc gtggcagcac caatacctta ccgctgcacg
     3481 tcgaaatttt atataacgaa tacaattttg ctgcggcgtt tgcagtcgct tcgctgttag
     3541 cactgttggc gctgatcacg ctggttgtca aaacattcat tgaatcaaaa gtttcccaac
     3601 aaaaaggcac tcatcatgag cattgaaatc cgtaatctcc ataaacaatt tggctcattt
     3661 actgcgctaa ataacgttag cttggatatt caaccgggtg aattattggc tttgctaggc
     3721 ccatcaggat cgggtaaaac caccttactg agagtaatcg ctggtctaga aacggttgat
     3781 tccgggcaag tactattcaa caatgaagat gcgacgcatc aacacattca tgaaaggcag
     3841 gttggatttg tttttcagca ttacgcatta tttcgcaata tgactatctt tgagaatgtc
     3901 gcatttggcc tgcgcgtgcg cccaaaatcg gttcgtcccg ataacgaaga aattaataaa
     3961 cgagtgacgg atctgctcaa attagtccag cttgattggc taggagatcg ttatccgcac
     4021 caactctcag gagggcagcg ccaacgcatt gcactcgcac gcgcgctcgc cgttgaaccc
     4081 aaagtattat tactcgatga accgtttggt gccctcgatg caaaagtacg taaagaatta
     4141 cgaacctggc taagaagatt gcacgatgat atgcatatca ccagtgtttt cgtgacccac
     4201 gaccaggaag aagcactcga agtatccgat cgggttgtgg tcatgaacga ggggcggatt
     4261 gaacaaattg caacacccga cgaagtctat gaaaagccag caaatccatt cgtttatgaa
     4321 tttttagggc atgtcaattt gtttcatggc cgcgttcaca aaggtcgtgc ttggattggc
     4381 gatatcgaga tcgacgcacc cgatcatgct gaggcagaag atttagcagc cgtggcttat
     4441 gtgcgcccac acgatatcga agtagaccga gaacccaatg gggaatcagc gctggctgca
     4501 cagatcgtgc atattctatc ggttgggcct attgtccgac tcgaactcgc gcgcgaggaa
     4561 aatcaaggta agaatttgat tcaggtagaa atcagcaaag aacgctttcg tggattacaa
     4621 ttggaaaaag gtgatcgagt tttcatcaaa ccgcgccgat tggatttgtt tccccatttg
     4681 acacactgag ttctttgtaa aagtcgtttg gctaaatagt cggtcctgca aaattaactt
     4741 aaccgtggcg gaggttttgt atacaagaaa g
//
LOCUS       DAOQKQ010000186         4748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_9999_length_4748_cov_5.749840, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000186 DAOQKQ010000000
VERSION     DAOQKQ010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4748
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9999_length_4748_cov_5.749840"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="PKZ49_10780"
     CDS             complement(<1..1036)
                     /locus_tag="PKZ49_10780"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HND37090.1"
                     /translation="MAFLSPDFSPAYPEIFLLIMICIVMLVDLFLDNDQRYFTYILSL
                     LSLAGCAVITFATLSSEVQYTFSGMFVDDLMSDSLKLMVYLTVAVVLIYSRNYIRVRG
                     MFKGEFFNLSLFATLGMMVMISASHFMVFYLGLELLSLSLYAMVALQRDSAVATEAAM
                     KFFVLGALASGFLLYGMSMIYGATGELHTSKIAEAIQTKTIDHQVLIIGLVFIVAGLA
                     FKLSAAPFHMWAPDVYQGSPTAVTLFLGSAPKLAAFGFTIRLLVDGLGALAADWQGML
                     IILAVASMAIGNIVAIMQTNIKRMLAYSTISHMGFVLLGFIAVDQNGYSSAMFYVIAY
                     VLMTLGAFGII"
     gene            complement(1087..2571)
                     /locus_tag="PKZ49_10785"
     CDS             complement(1087..2571)
                     /locus_tag="PKZ49_10785"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HND37091.1"
                     /translation="MLFGFPILSVVIWLPIIFGLFVLIIGNDHRVVLVRWISLLGSTL
                     GFIVALPLYTGFDSTLSSMQFVEQHIWFERFNVYYHLGIDGISMPLILLNCLITPLVV
                     IAGWEVIRDRTSQYFGAFLIMSGIVNGVFASLDAVLFYTFWEASLIPMFIIIGIWGGP
                     NRVYAAIKFFLYTLLGSLLMLVALLYLYHESEGSFSILSYHQLPLAMTPQILIFLAFL
                     LAFAVKVPMWPVHTWLPDAHVEAPTGGSVVLAAILLKLGGYGFLRFSLPIAPDASHYL
                     AEWMIILSLIAVVYIGLVALMQQDMKKLIAYSSVSHMGFVTLGFFLFNGYGIEGAMVQ
                     MISHGFISGAMFLCVGVLYDRLHSRQIADYGGVVNRMPIFAAFFMLFAMANAGLPGTS
                     GFVGEFMVIMGSVKVNFWYAFFAAMTLIFGAAYTLWMYKRVIFGEVANKNVEQLQDIS
                     LREGFLLTLLAMAVLWFGLYPFQLTEVMHATVDNLLEHLMNSKL"
     gene            complement(2667..4616)
                     /gene="nuoL"
                     /locus_tag="PKZ49_10790"
     CDS             complement(2667..4616)
                     /gene="nuoL"
                     /locus_tag="PKZ49_10790"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HND37092.1"
                     /translation="MKELYLLVPLAPLLGAIIAGLFGRFIGTTWSHRVTILLVFVSLI
                     TSLIIFQDVIQAGNIFNGTIYTWLVLGETEFEVGFLIDPLSVTMMVIVNLVSLMVHVY
                     TIGYMQGDPGYQRFFSYISLFTFSMLMLVMSNNFLQLFFGWEAVGLVSYLLIGFWYTR
                     PTAIYANLKAFLVNRVGDFGFLLGIGLVLAYFGTLDYEIVFAHAEYMAKHSIEIIPGQ
                     SWMLLTVICLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGIFMVAR
                     MSPLFELSETALSTVIVIGGITTLFMALVAVVQNDIKRVVAFSTLSQLGYMTVALGAS
                     AYSIAIFHLMTHAFFKAVLFLGAGSVIIAMHHEQDMRRMGGLKKYMPITYWTMFIAAL
                     ASAGVPGFSGFFSKDAIIEAARFTEIPGGGFAYFCVLTTVFVTALYTFRLMIMTFHGK
                     ERMDHHTREHLHESPWVVTVPLIVLAIPTVVSGWIIGSLVFGDYFGNSIYFLSEHETM
                     MKLEAEFSGTFGMMLHALGTAPFWLSVGGIFTAWYLYAYKTEIPSKIKSLSGPIYNLL
                     DRKYYIDELYSLIFATGTRSLGTVLWKIGDVKIIDGLIVNGSAKLVGWVASWVRFFQS
                     GYIYHYAFTMIVGIFILMSLWLYHS"
     gene            complement(4661..>4748)
                     /locus_tag="PKZ49_10795"
     CDS             complement(4661..>4748)
                     /locus_tag="PKZ49_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HND37093.1"
                     /translation="AAIGLAILVVLFRNLRTINVDDLSELKG"
BASE COUNT         1714 a         1036 c          800 g         1198 t
ORIGIN      
        1 tgattattcc aaatgcaccc aacgtcatga gtacataagc gataacgtag aacatagccg
       61 agctatagcc attctggtct actgcgataa aaccgagtaa aacaaatccc atatgcgaaa
      121 tcgtagaata agcaagcatg cgcttaatat tcgtttgcat aatcgcaacg atattaccaa
      181 ttgccatcga agcaaccgct aatataatta gcatgccttg ccaatcggct gctaaagcac
      241 caagaccatc aaccaataat cgaatagtaa acccaaatgc agctaatttt ggagcagaac
      301 ccagaaatag tgttactgca gtaggagacc cttgataaac atcaggtgcc cacatatgaa
      361 atggtgcagc actcaattta aaagccagac ccgctacaat aaaaaccaac ccgattatca
      421 atacttgatg atcaatcgtt tttgtttgga ttgcttcagc aattttgctc gtatgtaatt
      481 caccggttgc accgtatatc attgacattc catacaacag aaaaccagac gccaacgcac
      541 ctaaaacaaa aaacttcatg gcagcttctg ttgctactgc agaatcccgc tggagagcaa
      601 ccatcgcata taaagaaagc gataacaact caaggcctag ataaaaaacc ataaagtgac
      661 ttgcagaaat cataaccatc attcctaagg tagcgaataa agagaggtta aaaaactccc
      721 ctttgaacat acctcttaca cgaatatagt tacgagaata aatcagaacc acagctacgg
      781 tcagatatac catgagtttc agactatctg acattagatc atcgacaaac atccctgaga
      841 aagtgtactg gacttcactt gataaagtcg cgaaggtaat cactgcacaa cctgccaaac
      901 taagcagcga gaggatataa gtaaaatacc tttgatcatt atccaagaaa agatccacta
      961 gcataacgat acaaatcatt atgagtagaa agatttctgg gtatgctggt gaaaaatcag
     1021 gtgacaaaaa cgccatctat tatccttttt gatctaaatt cttgtatatt ttattaattc
     1081 gcaaggctac aacttactat tcattaagtg ttccagtaaa ttatcaactg ttgcgtgcat
     1141 tacttctgtg agctgaaatg gatacagtcc aaaccatagc actgccatgg ccaatagggt
     1201 tagcagaaaa ccctcgcgta atgaaatatc ctgaagctgt tctacatttt tattggctac
     1261 ttccccaaaa attacccttt tatacatcca caatgtataa gctgcgccaa aaatgagtgt
     1321 catcgcagca aaaaatgcgt accaaaaatt aactttcaca gaacccataa ttaccatgaa
     1381 ttctcccacg aaaccactag tcccaggcag tccagcatta gccattgcaa acaacataaa
     1441 aaatgcagca aaaatcggca tccgattaac aacaccacca taatcagcta tttgccgtga
     1501 atgtagtctg tcatacaaaa caccaacgca taaaaacatg gcacccgaaa taaaaccatg
     1561 agaaatcatt tgcaccattg caccttctat tccatagcca ttgaataaaa agaaacccaa
     1621 agttacaaaa cccatatggg agacagatga ataggcaatc aattttttca tgtcttgctg
     1681 cattaatgca acaagaccaa tataaactac tgcgatcagc gacaaaataa tcatccactc
     1741 agccaaatag tggcttgcat caggtgcgat cggtaatgag aatcttaaaa atccataacc
     1801 acctaatttc aataaaattg cagcaagcac aacagaacca cctgtaggcg cttctacgtg
     1861 tgcatccggt aaccatgtat gcactggcca catgggtacc tttacagcaa acgctaacaa
     1921 gaatgctagg aatataagaa tttgcggtgt catcgccaga ggaagctggt gataacttaa
     1981 tattgagaag ctgccttccg attcatgata caaatagagt aaggcaacca gcatcaacaa
     2041 cgaacctaag agtgtataca gaaaaaactt aattgcagca tatactcggt tgggaccacc
     2101 ccaaatccca ataataataa acattggaat caaggatgct tcccagaaag tatagaaaag
     2161 taccgcatcc aatgacgcaa acaccccatt aacgatccct gacataatca agaatgcacc
     2221 gaaatattgt gaggtgcgat cgcgtattac ttcccaacct gcaattacaa ctaaaggtgt
     2281 aattaagcag ttgagtagta tcaatggcat ggaaattcca tcaatgccaa gatgataata
     2341 aacattaaaa cgttcaaacc aaatatgttg ctccacaaac tgcatgctac ttaaagtgga
     2401 atcaaatccc gtatataaag gcagtgcaac aatgaaacct aaagttgaac ctaaaagaga
     2461 aatccaacgt actaatacca ctctatggtc atttccgatg attaatacaa agagaccaaa
     2521 gatgataggt aaccagatga ctacacttag aattggaaat ccaaacagca tgacaaatcc
     2581 acttgattaa aattagagtt tattattttt tctcttcgat gcaactaatg atagataaaa
     2641 ttgaagatag atagctgaaa tcaccattat gaatgataca accataaact catcaaaata
     2701 aaaatcccta ctatcattgt aaatgcgtaa tgataaatat aacctgattg aaagaagcga
     2761 acccacgaag cgacccatcc aaccaatttg gctgagccat ttacaatgag accatcaata
     2821 attttcacgt ctcctatttt ccacaacacg gttcctagcg atcgcgttcc agtggcaaat
     2881 attaaagaat aaagctcatc aatatagtat ttccgatcaa gtaaattata aattgggcct
     2941 gatagggatt taattttaga aggaatctct gttttatagg cataaagata ccaagctgta
     3001 aaaattcctc caactgaaag ccagaatgga gcagttccaa gtgcatgtag catcattcca
     3061 aaagtaccag agaattctgc ctccaatttc atcatcgtct catgctcaga taaaaagtaa
     3121 atcgaattcc caaagtaatc accaaacact aaagatccta taatccaacc tgagacaact
     3181 gttggtatcg ctaatacaat taatggaaca gttacaaccc agggagattc atgaagatgt
     3241 tcgcgcgtat gatgatccat gcgttcctta ccatggaagg tcataatcat caatcggaag
     3301 gtataaagcg cggtaacgaa tactgttgta aggacacaaa aataagcgaa tccccctcct
     3361 gggatttcag taaatcttgc tgcttcgata atggcatcct ttgaaaagaa ccctgagaaa
     3421 cctggaacac cggcgctcgc aagtgcagct ataaacatag tccagtaagt gatgggcata
     3481 tattttttta atccacccat tcttctcata tcttgttcat ggtgcatcgc tatgatcacc
     3541 gatcctgctc cgagaaatag taccgctttg aagaacgcat gcgtcatgag atgaaaaatt
     3601 gcgattgagt aggccgatgc tcctaaagcg acagtcatat acccaagctg cgatagtgtt
     3661 gagaatgcaa ccactcgttt aatatcattc tgaaccactg cgactagtgc cataaatagt
     3721 gttgtgattc caccaataac gataactgtc gataaagccg tttcagataa ttcgaaaagg
     3781 ggagacattc tcgcaaccat aaaaatgcct gcggtcacca ttgttgcagc atgtatcaat
     3841 gcggatatcg gtgtaggacc ctccattgaa tcaggtagcc atacatgtag tggaaactgt
     3901 gcagatttcc ccattgcacc tacaaataat aacaagcaaa ttacggtcaa taacatccag
     3961 gactgtccag gaataatttc gattgagtgc ttcgccatat attctgcatg cgcaaaaacg
     4021 atctcataat ctaatgtacc aaaataagcg agtactagac caattcctaa aagaaaacca
     4081 aaatcaccta cgcgattgac aagaaatgct tttaaatttg cgtagattgc cgtgggtctg
     4141 gtataccaga atccaatcaa taaatacgat actaatccta ctgcttccca accaaaaaat
     4201 aattgaagga aattattgga catcactaac atcaacattg agaaggtgaa tagtgatata
     4261 taactaaaga aacgttggta acctggatca ccttgcatat aaccgattgt gtaaacatgc
     4321 accatcagcg aaaccaaatt aacgattacc atcatcgtta ctgaaagcgg gtcaatcaaa
     4381 aaaccaactt caaattctgt ttcgcctaaa actaaccagg tataaattgt tccattaaaa
     4441 atatttccgg cctgaataac atcttggaaa ataattaatg atgtaatgag tgatacaaaa
     4501 accagcagaa ttgttacacg atgactccaa gttgttccaa taaagcgtcc aaataacccc
     4561 gctatgatgg ctcctagtaa tggcgccaat ggcactaaca aatacagttc tttcattgag
     4621 ctaataaatt aaatttttag taatctttta ataaaaatat ctaccctttg agttcactca
     4681 aatcatctac attaatcgtt cgaagatttc ggaacagcac tactaaaatt gcgagtccaa
     4741 ttgctgct
//
LOCUS       DAOQKQ010000187         4724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_10070_length_4724_cov_5.825659, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000187 DAOQKQ010000000
VERSION     DAOQKQ010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4724
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10070_length_4724_cov_5.825659"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            567..974
                     /gene="merR"
                     /locus_tag="PKZ49_10800"
     CDS             567..974
                     /gene="merR"
                     /locus_tag="PKZ49_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hg(II)-responsive transcriptional regulator"
                     /protein_id="HND37094.1"
                     /translation="MKNNLENLTIGAFAKAAGVSVETIRFYQRKGLLPEPDKPYGSIR
                     RYSRVNVQRVRFVKSAQRLGFSLNEISELLRLEDGIHCNEVSYLTEIKLKDVRKKLKD
                     LTRMEAALSALLRACHTRKGNVSCPIIASLQAD"
     gene            complement(1097..1363)
                     /locus_tag="PKZ49_10805"
     CDS             complement(1097..1363)
                     /locus_tag="PKZ49_10805"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HND37095.1"
                     /translation="MVTPDMHRIHHSTKEDEANSNFGFNLPWWDRLFGTYRDQPRGDY
                     QSMVIGISGYRDPRQVERLLGMLALPWSGDILSYAINRRNRDQK"
     gene            complement(1415..2634)
                     /locus_tag="PKZ49_10810"
     CDS             complement(join(1415..2349,2349..2634))
                     /locus_tag="PKZ49_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611570.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HND37096.1"
                     /translation="MKNQISYSPEVRERAVRLVFEQQKEHESQWSAIKSIASKIGCTP
                     ETLRTWVRRTETDQGIRAGMSTADRERLKELEQENRELKRANEILRKASAYFCAGGAR
                     PPTEIMVTFVDSNKAEYGVEPICNEIWIAPSSYYEHKARERDPDRLPDRIKRDMRLEL
                     DIQRVWKDNFRVYGARKVWRQLLREGIGVARCTVERLMKKLGMQGARRGKKCWTTIAD
                     DSLCRPTDKVNRQFVAARPNQLWVADITFVATWIGFVYVAFITDVFARYIVGWRVSRS
                     LRTDLVLDALEQALWARRETAGLIHHSDHGCQYLSIRYTERLAQANIDASVGSVGDSY
                     DNDMAETINGLYKTEVIRHRGPWRSIEEVEFATLEWVDWFNNRRLLDAIGYVPPAEFE
                     KAYYRQQEESADAA"
     misc_feature    complement(2240..2356)
                     /locus_tag="PKZ49_10810"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            2723..3469
                     /locus_tag="PKZ49_10815"
     CDS             2723..3469
                     /locus_tag="PKZ49_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HND37097.1"
                     /translation="MIERQWQQGDWQNAGQGFDAVEGKLRLTSWSGARRVVVLRRRIK
                     DSMAAAETSNENLQPMLHFIDHSDKVKLWEYAILVTNTNYSLDAIGQLYRDRADCENG
                     FDELKNQWGWGGYTTHDLERCNLSARAVALIYNWWSWYVRLAHPKTRLEAITSRPMLL
                     SGIARLTQHAGQSRLLLTLTHAAGDHIKSMITNIRKGLDHVLANAPQLTKADRWGVLV
                     RYIINKIIATRPKNSQLLGFPPPSLSIGVT"
     gene            complement(3723..4073)
                     /locus_tag="PKZ49_10820"
     CDS             complement(3723..4073)
                     /locus_tag="PKZ49_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HND37098.1"
                     /translation="MTRTLFILNDAPYGNERVYNALRLAGALVNKEDQHVRVFLLADA
                     VTCAKAGQKVPEGYYNVQLMLRKLLQKGELAMCGTCMDARGMTEAEILEGVRRSTLNQ
                     LADWTIEADKVLVF"
     gene            complement(4338..>4724)
                     /locus_tag="PKZ49_10825"
     CDS             complement(4338..>4724)
                     /locus_tag="PKZ49_10825"
                     /inference="COORDINATES: protein motif:HMM:NF022818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3179 domain-containing (seleno)protein"
                     /protein_id="HND37099.1"
                     /translation="RNRHPDTLVLDTNTGFNRNYQANPYDGYERESSIMFPVRFRAEG
                     YHPKEQVLGLVLDGKAKAYPFVELAKGSGEVSDVLAGKNIHVRYSHNHKSAEVFDSEG
                     NALPGITLFWFAWYAFHPETEIYRVK"
BASE COUNT         1237 a         1243 c         1029 g         1215 t
ORIGIN      
        1 aaaaatttca cagataccgt aaaaaaggat aggcaggaag aaataaagca cgtttgggga
       61 cgtgtctaga ttctatagtt agaaatttag atccggccct ttccgcacgc tagtcaatcc
      121 ggcggcacat atccgcacat tttctgcaca tctcggcaca tttctggcaa tgttcttcgt
      181 catgtttctc acattcttcc gcgcataatt cacatatccc ggcacagagc gcgcatattt
      241 tcttggaaat acttgaatta cgggccataa ggcgagcgca aagctggcaa atttccgcgc
      301 agtccataca taggcgcaag catttctctc tgtcagaacc gatacagcag tctacacact
      361 cctcacaggc catgaagcaa gccaaacagg cttcaatgca attctgataa tttctatttt
      421 tcatgatatt acctccttgt tttgcagtaa aaatgggtta acaattcaat agtcttagga
      481 agttaagccg gtcatacatt tctcctttta aaaattgcac aggtaccgta agattacttc
      541 cgtagttatg tacggagtca agcggtatga agaataatct ggagaatctg accattggcg
      601 catttgccaa ggcagccggg gtcagtgtgg aaacgatccg cttctatcaa cgcaaaggtt
      661 tgctgcccga gccggacaag ccctacggca gcattcgccg ctatagcaga gttaatgtgc
      721 agcgcgtaag gtttgtaaag tccgcccaac ggctgggttt cagtttgaat gaaatcagcg
      781 aattgctacg gctggaagac ggcattcact gcaatgaagt cagctatttg actgaaataa
      841 aactaaaaga cgtgcgtaaa aaactgaaag atctgacgcg catggaggct gcgctatccg
      901 cgcttttacg cgcctgccat acacgaaaag gaaatgtttc ctgcccgatt attgcatcac
      961 tgcaagcaga ctgagccaag gtagcagaag attaaccagg gttaacgctg aattttttca
     1021 catccatttc aatctgaaat aaccaagatc gtaagcatgc aaaaataaag gaatttctga
     1081 aaaataaatc tgttacttat ttctgatcac ggttacgccg gttgatggca taactgagaa
     1141 tatctccgct ccacggcagt gccagcatac cgagcaaacg ctcgacttgt ctcggatccc
     1201 ggtagccgct gatgccaatt accatgctct gataatcgcc tctgggctga tcccgataag
     1261 tgccgaataa tctatcccac caagggagat tgaaaccgaa gttactgttc gcttcgtctt
     1321 ctttggtcga gtgatgaatg cgatgcatgt ccggcgtaac cagaacccag cgcagtgaat
     1381 cgccccggta tttccggaga catgttttct tgagtcaagc tgcatcagct gactcttcct
     1441 gttggcgata atacgccttt tcaaattctg ccggtgggac atatccgatt gcatccagca
     1501 gtcttcgatt gttgaaccaa tccacccatt ccaatgtagc aaattctact tcttcgatac
     1561 tacgccaagg accgcgatgc cgtatgactt cggtcttgta caagccattg atggtctcgg
     1621 ccatgtcatt gtcataagaa tctccgacgc tgccgacgga ggcgtcaata tttgcctgcg
     1681 ccaaacgttc cgtatagcga atcgacaaat attggcaacc gtggtcgcta tgatgaatca
     1741 atcctgcggt ttcccgccgt gcccatagcg cctgttccaa tgcatccaat accagatccg
     1801 ttcgcaatga acgactcact cgccaaccaa caatataccg ggcaaaaaca tcggtgataa
     1861 aagccacata aacaaagcca atccacgtcg ccacaaatgt aatatccgcc acccacagtt
     1921 gattgggccg ggcagctaca aattgccggt taaccttgtc tgttgggcgg caaagtgagt
     1981 catctgcaat ggtcgtccaa cactttttgc cgcgtcgagc accttgcatt ccaagcttct
     2041 tcatcaaccg ctcgaccgta caacgagcaa caccaatacc ttctcgcagc aactgccgcc
     2101 atactttgcg agcaccataa actcggaaat tgtctttcca tacccgctgt atatcaagtt
     2161 ctagtctcat gtcccgcttg atgcgatcag gcaatcgatc cggatctcgc tcgcgtgctt
     2221 tgtgttcgta ataactcgac ggggcaatcc agatttcatt gcagattggc tcgaccccgt
     2281 attctgcttt gttgctatcg acaaatgtca ccattatttc ggtcggcggt cgagctccgc
     2341 ctgcgcaaaa taggccgatg ccttacgtaa tatctcgttg gctcgcttta attcccgatt
     2401 ctcctgttcc aattccttga gtctctcacg atccgcagtc gacatgcctg cacgaatccc
     2461 ctgatcagtc tccgtccgcc ttacccaagt ccgcaatgtc tccggtgtac acccaatctt
     2521 tgatgcgatc gatttgatcg ccgaccattg cgattcatgc tccttttgct gctcaaatac
     2581 caatcttact gctcgttctc gtacttccgg tgaataactg atttgattct tcatctcttc
     2641 ctcctctcaa atcattttat ctccggaaat atcggggcga ttcaacctct tcaagctacg
     2701 acaaactgcc ggagttaagc gtctgatcga gcggcaatgg cagcaaggtg actggcagaa
     2761 cgcagggcaa ggctttgacg cggtggaagg caagctccgg ctgacgagct ggagtggtgc
     2821 gcggcgtgta gtggtactgc ggcggcgaat caaagacagc atggcggctg ccgagacgag
     2881 caatgagaac ctgcagccga tgctccactt cattgaccat tcagacaagg ttaaactctg
     2941 ggaatacgct atattggtga ccaatacaaa ctactccctc gacgccattg gccagttata
     3001 ccgggatcgg gccgactgcg agaatggctt cgatgaactg aaaaaccaat ggggttgggg
     3061 tggctatacc acgcatgacc tggaacgttg caacctctca gcgcgagcag tcgcgctcat
     3121 ctacaattgg tggagttggt atgtcaggct ggcacacccg aagacacgcc tggaggcaat
     3181 cactagccgt cccatgttgc tcagcggcat tgcccgcctg acccagcatg ccggtcaatc
     3241 ccgattgctg ctcactctga ctcacgcagc cggagatcat atcaagtcga tgatcaccaa
     3301 tatacgaaaa ggtcttgatc atgttctggc aaatgcgcct cagttgacca aagcggatcg
     3361 ttggggcgta cttgtacgct atatcattaa caaaattatt gcgacaagac cgaaaaattc
     3421 acaactcctc ggcttccctc caccctcttt gtccattgga gtgacctaac agaggaatct
     3481 aggttaatcc agcgaagact cttcgaaata gtcaaggcac gacaaggcgc cagttgttcc
     3541 caagccgcca tgaggaaaaa cacaccccaa aaaacagtca atttaacaat gagttcgtta
     3601 tggataatcc agtcgttcca gttcataccg cgcttcctga gccaagttca attgcaacaa
     3661 caggacagag aagatactca agctgtcaat tgctaaactt aaagagcatc gttgttattc
     3721 ctttaaaata caagcacctt atcggcttca atagtccagt cagctagctg gttcagtgtg
     3781 gaacgcctca cgccctccaa tatctccgcc tcggtcatgc cacgcgcatc catgcaagtg
     3841 ccacacatgg ccaattcacc cttttgcagc agcttgcgca acatcaactg aacgttataa
     3901 tacccttccg gcaccttctg cccggccttg gcgcaagtca ccgcatcggc cagcagaaac
     3961 acccgcacat gctgatcttc cttgttgaca agcgcacctg ccagacgcag tgcattgtaa
     4021 acccgttcgt tgccgtaggg cgcgtcattg agaataaaaa gtgtacgagt catgcttgtt
     4081 tcctccgttt tgccatcgct actcggcaag ttatggttct ttatacgaac taatccatct
     4141 cctgccattc acataccttg cccagaatat ggctattgtg taaagctatg ccgcattttg
     4201 ctattgatga tttttctgta actatataat tctaatgaag ttaagtgaaa tataaaaaaa
     4261 tgcacaagac cacgatggat agtagtctgg tcgattttta aatttccttc aatgattaat
     4321 tgcagcatag ccatatatta cttgactcga taaatctcgg tttccggatg aaatgcatac
     4381 catgcaaacc agaataacgt gatgccgggc aatgcgttgc cttctgaatc aaaaacttcg
     4441 gcagatttat ggttgtggct atagcgtaca tggatatttt ttccggccag tacgtcactt
     4501 acctcgccag aacctttagc cagttccaca aatgggtaag ccttggcttt tccgtctaaa
     4561 accaaaccta gtacctgctc tttgggatga taaccttctg cacggaagcg taccggaaac
     4621 atgatgctgc tttctctttc atatccgtca tagggatttg cctggtaatt gcggttaaaa
     4681 ccagtattcg tatccagcac cagcgtatct ggatggcgat tacg
//
LOCUS       DAOQKQ010000188         4724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_10072_length_4724_cov_5.316128, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000188 DAOQKQ010000000
VERSION     DAOQKQ010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4724
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10072_length_4724_cov_5.316128"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..1795)
                     /gene="ureC"
                     /locus_tag="PKZ49_10830"
     CDS             complement(89..1795)
                     /gene="ureC"
                     /locus_tag="PKZ49_10830"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HND37100.1"
                     /translation="MSVKISRQTYAEMMGPTTGDRVRLADTELFIEIEKDFTTYGEEV
                     KFGGGKVIRDGMGQSQRNHADVMDTVITNAVIVDHWGIVKADIGLKNGKIAAIGKAGN
                     PDIQPNVTMAIGSATEIIAGENMIVTAGGVDTHIHFISPQQAEDAMMNGITTMLGGGT
                     GPAVGTAATTCTPGPWHMHSMLRASDGIVMNTGFFGKGNTSLPTPLEEQILAGGCGLK
                     LHEDWGTTYAAIDNCLAVADKYDVQVAIHTDTINEGGYLENTIAAMKDRTIHTFHTEG
                     AGGGHAPDIIAVVGLDNVLPSSTNPTRPYTVNTLDEHLDMLMVCHHLHANIAEDLAFA
                     ESRIRKETIAAEDILHDMGAISMMSSDSQAMGRIGEVVLRTWQTAHKMKIQRGTLQED
                     NSRNDNFRVKRYVAKYTINPAITHGIGHAIGSVEVGKYADLVLWRPGFFGVKPSMILK
                     GGMIAASLMGDPNASIPTPQPVHYRYMFGGYGGGIKTSCFTFASQAALSAGLVDQLKL
                     DKNIVAVKNTRNLRKKDMIHNSATPKMEVDPETYEVRADGQLLTCGAEDVLPMAQRYF
                     LF"
     gene            complement(1828..2148)
                     /locus_tag="PKZ49_10835"
     CDS             complement(1828..2148)
                     /locus_tag="PKZ49_10835"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HND37101.1"
                     /translation="MRLPLIPGEVFTEAGEIELNVGRKTKTLTVSNGGDRPIQIGSHF
                     HFYEVNSAMKFDREAAYGMRLNIMAGTAVRFEPGQERTVELVELAGDRVVYGFNQKVM
                     GKLK"
     gene            complement(2171..2473)
                     /locus_tag="PKZ49_10840"
     CDS             complement(2171..2473)
                     /locus_tag="PKZ49_10840"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006811999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HND37102.1"
                     /translation="MDLTPREKDKLQIFTAGLLAERRKARGLRLNYPEAVALITCAVL
                     EGARDGRTVAELMTAGQKVLSRDEVMEGVPEMIPDIQVEATFPDGTKLVTVHNPIV"
     gene            complement(2561..3511)
                     /locus_tag="PKZ49_10845"
     CDS             complement(2561..3511)
                     /locus_tag="PKZ49_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HND37103.1"
                     /translation="MQFSTQTGVPLTHQHNSNVLTYPSGKQSAEHQPKSVMKPVHNPL
                     QARLSMRFGHFDGVTRLVEREHYGPLLIQKPLYPEGRGICHAVIIHPPGGVVGGDELA
                     IAARVDASAKAQITTPGAAKWYKANGFTSHQEININVAKGGALEWVPQETIFYNHSNV
                     KIDHQVALEDDAVYVGCEILCFGRTAYGETFDEGQIKQRTRIIRNGKMIWLEQICLKG
                     ENATMKGALALSGYTVCATLLLTGKTIAQPLIDLARLEAERLTNKTGQVGISQLKSVV
                     VVRYLGHSSEVARQVMLSVWGLFRPEVLGYPAIVPRMWTT"
     gene            complement(3556..>4724)
                     /locus_tag="PKZ49_10850"
     CDS             complement(3556..>4724)
                     /locus_tag="PKZ49_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37104.1"
                     /translation="VIKILDSNGNRFARQLLKLGAGFRGTGLWVENQNSGNLGGGFNV
                     DNARLYLNGQFHQYLKFEVNTECYFCDNTASKDNPKMSYNILDAIAKFEFNRYFNVWG
                     GRMLAPTERGELNGPFFHATHDGFKTPFFSQDFSTKYGSGGAGMYGRDDGATLWGSVE
                     PGIVPGTFSYAMGIFRGLRSSTGEGPNPGGNVLTAARFTYNFLNPEKNPGYYTAGTYY
                     GKAGDILALAFGMTYQKDGAGSLAHRSDFLGFVGDMLFEKVLPQNMGVLTANAEYKQF
                     YANYSSAAFNDKDCFCMFDGKSWTVTGLYLIPTKIGIGQFQPYGRYTSVQPNRSSNRD
                     EIEAGLNYIIDGFNARISAYYQHGDLFTRGLNYAPTGISGRAVDVFKISFQLQI"
BASE COUNT         1292 a         1216 c          922 g         1294 t
ORIGIN      
        1 agtcactgcc aaatagcgca gtccattacg ctatgactaa acctaatagg ttagttaatg
       61 catgaactgc gcaacttgaa cgctaccgct agaataagaa ataccgttgt gccatcggca
      121 atacgtcttc tgcgccacag gtgagtaatt gcccgtcagc acgaacttca taggtttccg
      181 gatcaacttc catcttcggt gttgcgctat tgtgaatcat atctttctta cgcaaattac
      241 gcgtattttt gaccgcaaca atatttttat ccagcttgag ttgatcgact aaaccagcac
      301 tcaacgcggc ttgcgatgca aacgtaaagc acgatgtttt aattccccca ccatacccac
      361 cgaacatata acgataatga acgggctgtg gtgttgggat cgatgcatta ggatcgccca
      421 tcaatgaagc tgcaatcatg ccacctttca ggatcataga tggtttgacg ccaaagaaac
      481 caggtcgcca gagcactaag tcagcatatt taccaacttc gactgaaccg atcgcatgcc
      541 caatcccatg ggtaattgca gggttgatgg tgtatttcgc aacataacgt ttcacgcgga
      601 aattatcatt ccttgaatta tcttcttgca atgtaccgcg ttgtattttc attttgtgcg
      661 ctgtttgcca agtccgcaat accacttcac caatccgtcc catcgcttga gaatccgatg
      721 acatcatcga aatagcgccc atgtcatgca gaatatcttc tgctgcaatg gtttccttac
      781 gaatacgaga ctcagcaaaa gccaaatctt ccgcaatatt ggcatgcaaa tgatggcaaa
      841 ccatcaacat atctaaatgc tcatccaggg tattcacggt ataaggacga gttggattcg
      901 ttgaagaagg cagaacatta tcgagcccca caaccgcgat aatatcgggt gcatgccccc
      961 cacccgcacc ttcagtatgg aaggtatgaa tggtacggtc tttcattgca gcaatggtat
     1021 tctctaaata accaccttca ttaatcgtat ccgtatggat cgcaacttga acatcatact
     1081 tatctgctac tgccaggcaa ttatcaatcg cagcataagt cgttccccag tcctcatgca
     1141 gcttgagacc acaaccaccg gctaaaattt gttcttcaag cggggtgggc aaactggtat
     1201 ttcctttacc gaagaaaccc gtattcatca cgattccatc agaagctctc agcatcgaat
     1261 gcatatgcca agggccagga gtacaagtgg ttgccgcagt accgacagcg ggacctgttc
     1321 caccgcctag catcgtggta atgccattca tcatggcatc ttcagcttgt tgcggagaaa
     1381 tgaagtggat atgcgtatca acaccacctg cagtgacgat catattttca ccagcgatga
     1441 tctcagttgc cgaaccgatc gccatcgtga cattcggttg aatatctggg ttacctgctt
     1501 taccgattgc ggcaatcttg ccattcttaa ggccaatatc cgctttgaca attccccaat
     1561 gatcaacaat gaccgcattg gtaatcacgg tatccatcac atccgcatgg ttacgctgcg
     1621 attgccccat tccatcccgt attactttac cgcccccaaa ctttacttcc tcaccatagg
     1681 tcgtaaaatc tttttcaatc tcaataaaaa gctctgtgtc agcaagccgt acacggtcgc
     1741 ctgttgtcgg gcccatcatt tctgcatagg tttgacggga aatttttacg ctcatttttt
     1801 tgctcctcta aaaataatcc ggtacgacta cttaagtttg cccatcactt tttgattgaa
     1861 tccatagaca actcgatctc ctgccaactc cacgagttca acggttctct cttgtccagg
     1921 ctcaaaacgg acggctgtgc cagccataat attgaggcgc atgccataag cagcttctcg
     1981 atcaaatttc atcgcagagt taacttcata aaaatgaaaa tgtgatccaa tctgaatggg
     2041 gcggtctcct ccatttgaaa ccgtaagtgt tttggttttt ctgccaacat tcaactcaat
     2101 ttccccagct tcggtaaata cttcacctgg tattaaaggt aatctcataa cgatctccta
     2161 tcttgatcaa ttacacaatt ggattatgga ccgtaaccaa cttggttcca tccggaaacg
     2221 ttgcttctac ttggatatca ggaatcattt ctggaacccc ttccatcact tcatcccgag
     2281 acaatacttt ttgccccgcc gtcatcaatt cagcaacggt acgcccatct ctagcgcctt
     2341 caagtactgc gcaggttatt aatgccactg cttccggata attgagcctt aagccgcgcg
     2401 cttttcttct ttcagcaagt aaccctgccg taaatatttg taatttgtct ttctctcttg
     2461 gtgttagatc catctcattt ctcctgagta aaaaatattt tcagatgatc ggcactactt
     2521 aacattaaac aatcaacagc ttccctacgt aaaaccgagc tcatgtcgtc cacatccgcg
     2581 gcacaattgc tggataaccc agtacttctg gtcgaaataa cccccatacg cttaacatga
     2641 cctgacgggc cacttcactt gagtgaccaa ggtaacggac tacaaccacg gatttaagct
     2701 gggaaatacc cacctgccca gttttattgg ttaatctttc cgcttccaat cgtgccagat
     2761 caattaaagg ctgcgcaatg gttttaccag tcagtaataa cgtagcgcag acggtatagc
     2821 ctgaaagtgc caaagccccc ttcatcgttg cgttctcgcc ttttaaacag atctgctcta
     2881 gccaaatcat tttcccattc cggattatac gagttcgttg cttaatctga ccctcgtcaa
     2941 atgtttctcc ataggcagtt cgaccaaaac ataagatctc gcaaccaaca taaactgcgt
     3001 catcctccag tgccacctga tgatctatct tcacattgga atggttataa aaaatcgtct
     3061 cctggggaac ccactctaaa gcgccacctt tagcaacatt gatgttaatc tcctgatgag
     3121 aagtaaagcc attggctttg taccattttg cggcaccagg cgtcgtaatt tgggctttcg
     3181 cagaggcatc gacgcgagca gcaattgcta gctcatcccc ccccactacc ccaccgggcg
     3241 gatgaataat gaccgcgtgg caaatgccac gtccttcggg atagagtggc ttctggataa
     3301 gcagcggccc ataatgttca cgctcgacta accgtgtgac accatcaaaa tgaccaaaac
     3361 gcatcgataa tctggcttgt aatggattat ggacaggttt cattacagac ttaggctggt
     3421 gctcagcgct ctgcttgcca ctgggataag ttaacacatt tgaattatgc tgatgagtca
     3481 acggtacccc tgtttgtgtt gaaaattgca ttatttaaat ttcttgtaaa ttgactaagc
     3541 aataacgaga aaacctcaaa tctgtagctg gaatgaaatc ttgaacacat cgactgctcg
     3601 tcctgatata cccgtaggtg cataattcag tcctctcgtg aagaggtcac catgttgata
     3661 atacgctgag atacgggcgt taaacccatc gatgatgtaa ttcagccctg cttcgatttc
     3721 atcgcggtta ctgctgcggt taggctgcac gctggtatat ctaccgtagg gttggaactg
     3781 tccaatacca attttggtcg gaatcaaata aagaccggtt acagtccatg atttaccatc
     3841 aaacatacaa aaacaatctt tgtcgttaaa tgcggctgat gaatagtttg cgtaaaattg
     3901 cttgtattct gcgttagccg ttagcacccc catattctga ggcaatactt tctcgaacaa
     3961 catatcaccc acaaaaccta agaaatcact acgatgcgcg agagaacctg cgccatcttt
     4021 ttgataggtc ataccgaaag caagcgccaa aatatcgccc gctttaccat agtaagtacc
     4081 ggccgtgtaa taaccgggat ttttctctgg atttagaaag ttataagtaa accgggctgc
     4141 cgttaaaaca ttgccacctg gattgggtcc ctctcctgtc gaagatcgga gtccacggaa
     4201 gatacccatc gcatagctaa acgttcctgg aacgattccc ggttcaacac ttccccataa
     4261 agtcgcacca tcgtcacgac catacatacc ggctccaccc gatccatact ttgtactgaa
     4321 atcctgggag aagaatggtg ttttaaaacc gtcatgggtt gcatggaaaa aaggcccatt
     4381 caactcaccc cgttcggttg gggccagcat tcggcctccc caaacattaa aataacgatt
     4441 aaactcgaat ttagcgatcg catctaaaat gttgtaagac attttaggat tatcctttga
     4501 agctgtgtta tcgcagaaat aacactctgt gttcacttcg aacttcaaat actggtgaaa
     4561 ctgcccattc aaataaagac gggcattgtc tacgttgaat cccccgccaa gattgcctga
     4621 gttttggttt tcaacccata gcccagttcc acgaaaaccg gcacccagtt ttagaagctg
     4681 tctggcaaaa cgatttccat tagaatcaag gattttgata acaa
//
LOCUS       DAOQKQ010000189         4683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_10220_length_4683_cov_5.413570, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000189 DAOQKQ010000000
VERSION     DAOQKQ010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10220_length_4683_cov_5.413570"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PKZ49_10855"
     CDS             complement(<1..299)
                     /locus_tag="PKZ49_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HND37105.1"
                     /translation="MKALVTGANGFVGSAVARTLLAAGHEVRTLVRAGSDRRNLTALP
                     VEIIEGDLRDVPSLKRAVKGCDSLFHVAADYRLWVPNPAVMYDINVQGSLSLLLAG"
     gene            complement(300..1361)
                     /locus_tag="PKZ49_10860"
     CDS             complement(300..1361)
                     /locus_tag="PKZ49_10860"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HND37106.1"
                     /translation="MASQIRSAPATVADQHYQNHILQGVSRTFALTIPQLPPALFSVI
                     GNAYLLCRIVDTVEDDNGLTSAQTRQFAEMFIQVVSGEVPAETFAEGLFPLLTDHTIP
                     AEHDLIKNTPAVIRITHSFNPVQRAALERCVRIMGKGMADYQETESLAGLKDLPAMNQ
                     YCYYVAGVVGEMLTELFCDYSPEIACNRATLMPLAVSFGQGLQMTNILKDIWEDRKRG
                     ACWLPQAIFTETGFDLNALRPGCTDPRFHAGLTRLIGVAQAHLRNALAYTCLIPAKEK
                     GIRRFCLWALGMAVLTLNKINQNHAFMHGNQVKISRRSVKATVLLTSVLAGQDWALQR
                     LFDWTARQLPQYDVLGNPR"
     gene            complement(1404..2657)
                     /gene="lysA"
                     /locus_tag="PKZ49_10865"
     CDS             complement(1404..2657)
                     /gene="lysA"
                     /locus_tag="PKZ49_10865"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HND37107.1"
                     /translation="MSGFAYFDYLSDQLQIESVALSAIAQEFGTPCYVYSKAALTAAY
                     QTFDQAFAGRDHLICYAVKANSNLAILQTLAKLGSGFDIVSGGELQRVLKAGGDPRKI
                     VFSGVGKSSDEICLALQTRILCFNVESEMELLRLNHIAAQCQRIAPVSLRVNPDVDAK
                     THPYISTGLKESKFGIPMADAERIYRAAQQLSNIHIAGLDCHIGSQLTELDPFIEASQ
                     KMLALLDRLAQQGIAIEHLDLGGGLGIRYTQETPPVIGDYVRALCASVGIRPQRIVLE
                     PGRAMVGNAGILLTRVEYLKHAPHRNFAIVDAAMNDLMRPALYDAHHDIIPVVKKAGQ
                     AQTYQVVGPVCETGDFLGHDRALYLEANDLLAVMSAGAYGMSMSSNYNTRPRAAEVMV
                     DGDHVHLIRARETIDHLLTGEELLR"
     gene            complement(2654..>2843)
                     /locus_tag="PKZ49_10870"
     CDS             complement(2654..>2843)
                     /locus_tag="PKZ49_10870"
                     /inference="COORDINATES: protein motif:HMM:NF025012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HND37108.1"
                     /translation="RCNSFACVNIASFSLLIIHVHRYCLIVLLTIGLIACGLKGPLYL
                     PDEPPPKIKDTPEDQVKP"
     assembly_gap    2844..2943
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2944..3337)
                     /locus_tag="PKZ49_10875"
     CDS             complement(<2944..3337)
                     /locus_tag="PKZ49_10875"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HND37109.1"
                     /translation="MKILIIGLSLLVGCSFSSNKPTSLALYDLGLKPVSEVQVAPVDV
                     SIMMTEITAPIWLTSPAMQYRLAYHDPARTYHYAHSRWVTAPAKLLTRRIQDDLSQLT
                     RKGVISSQEGLKSDYALHIELEEFTQVFD"
     gene            complement(3347..4237)
                     /locus_tag="PKZ49_10880"
     CDS             complement(3347..4237)
                     /locus_tag="PKZ49_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HND37110.1"
                     /translation="MENRAHALAAGIFVIILGVATGYAVRWFSNQDILRDRYLLVSEA
                     GSVSGLNPAAVVRYRGVSIGKVEEIRFDTQDQTFILVGIAVNSDIKLPTNIYAQLANQ
                     GLTGLAYIELNTEGDPEETYLPSNARIELRASFIRKLSDSVENILNNTNKAVKRLNAL
                     MNQKNQEQFSAILANLAQAIQRYGSLVEQLQPGLKSLPTLSAEANRVMKQTHQLLAEV
                     NQTIVKTNQPGGLSDGLTQSVQAFADSLPKLREATESITQSTHHINRLLQRLEEHPQS
                     LLFGAPIATPGPGETGFHSP"
     gene            complement(4362..>4683)
                     /locus_tag="PKZ49_10885"
     CDS             complement(4362..>4683)
                     /locus_tag="PKZ49_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37111.1"
                     /translation="AVCLMYSSILTQSINWRSMMKLVFYVVLITLGLTLATVHATETV
                     PEKVEATSKELKREAKKTVNRAEEALCTESEAECLKRKMKNRTEETWDTLKDKATDLK
                     NKLD"
BASE COUNT         1218 a         1144 c         1049 g         1172 t
ORIGIN      
        1 cccgccagca gcaatgatag cgaaccttgc acattgatgt cgtacatcac cgcagggttg
       61 ggaacccaaa gccggtaatc agccgccaca tgaaataggc tatcacaccc tttgaccgcg
      121 cgttttagcg atggaacatc acgcaaatca ccttcgatga tttcaactgg tagcgccgtg
      181 agattacgcc gatcgctgcc agcccgaacc aaagtgcgca cttcgtgtcc cgctgccagt
      241 aatgtcctgg caacggctga gccgacaaac ccatttgccc ccgtgaccag cgctttcatt
      301 taacgtggat tccccagcac atcatattga ggaagttgcc gtgcagtcca atcgaacaga
      361 cgctgcagcg cccaatcttg cccagccagc acactggtaa gcaataccgt agctttgaca
      421 ctgcggcggg aaatcttgac ttgattgccg tgcatgaaag cgtggttctg attgatctta
      481 ttgagcgtca gcaccgccat acccagcgcc cacaggcaga aacgccgtat acctttttcc
      541 ttggctggaa tcaagcaggt ataagccaac gcattgcgta gatgggcttg agccacgccg
      601 atcaaacgcg tcaagccggc atgaaagcgc ggatcagtgc aacctgggcg caatgcgttg
      661 agatcaaacc cggtttcagt aaaaatcgct tgcggtaacc agcaagcgcc gcgtttgcga
      721 tcttcccaaa tatccttgag aatgttggtc atttgcaacc cttgtccaaa ggataccgcc
      781 aacggcatca acgtagcccg attgcacgca atttccggtg agtaatcaca aaacaactcg
      841 gtcaacattt caccaaccac gccggcaacg taataacaat attggttcat cgcgggcaga
      901 tctttgagac ccgccagcga ttcagtttct tgataatcgg ccatgccctt gcccatgata
      961 cgcacgcagc gttccagcgc agcgcgttgc acgggattga aactatgcgt gatacggatg
     1021 accgccggag tatttttgat gagatcgtgc tcggctggaa tggtgtgatc agtcaggagt
     1081 ggaaataatc cttcggcaaa ggtttccgcg ggtacttcac cactcacaac ttgaatgaac
     1141 atttcagcaa actgtcgcgt ttgtgcggaa gttaatccat tatcgtcttc cacggtatcg
     1201 acgatacgac acagcaaata cgcgttgcca atcactgaga ataacgccgg tggcaattgc
     1261 ggaatggtga gtgcaaaagt acgggaaacc ccttgcaata tgtgattctg gtaatgctga
     1321 tctgcaacgg tggccggtgc tgatcgtatt tgactagcca tgatcggtaa cggagctcaa
     1381 taaaatcaac atcctgatca accctaccgc aacaattctt cgccggttag caaatgatcg
     1441 atagtctcgc gcgcgcgaat caaatgtaca tgatcgccgt caaccatgac ttccgccgca
     1501 cgcgggcggg tattgtaatt ggaactcatg ctcatgccgt aggcgcctgc cgacatcact
     1561 gccaataagt cattagcttc gagataaagt gcacgatcat ggccgagaaa atcacccgtt
     1621 tcgcaaaccg ggccgaccac ttgataagtt tgcgcttgcc ctgctttttt tacgactggg
     1681 ataatgtcgt gatgcgcatc atacagcgca gggcgcatta aatcattcat cgcggcatcg
     1741 acgatcgcaa aattacgatg cggtgcatgt ttgagatatt caacccgtgt cagcaaaata
     1801 cccgcattgc ctaccattgc tcggcctggt tcaagcacga tgcgctgcgg acgaatccca
     1861 acgctggcgc acagtgcgcg cacatagtca ccgatcaccg gtggagtttc ttgggtatac
     1921 cgaataccga gacctccccc gagatcgaga tgttcgatag caataccctg ttgcgctaaa
     1981 cgatcaagta gcgccagcat tttctgactg gcttcgataa atggatcgag ttccgtgagt
     2041 tgagaaccaa tatggcaatc aagcccggct atatgaatat tagagagctg ttgtgctgca
     2101 cgatagatcc gttcggcatc tgccatcggt atcccaaatt tgctttcctt gagtcctgtg
     2161 gaaatataag gatgtgtctt agcatcgaca tcaggattaa cccgcaaact cacgggcgcg
     2221 atgcgctgac actgtgccgc aatgtgattg agccgcaata gttccatctc ggattcgaca
     2281 ttgaaacata aaatcctcgt ttgcaatgcg agacagattt catcgctcga tttaccaacg
     2341 ccagaaaata cgatttttcg gggatctcca ccggctttca agacgcgttg tagttctcca
     2401 cccgatacga tatcgaaacc actgcctaat ttcgctaatg tttgcaaaat tgccagattt
     2461 gaattagctt tgacggcata gcagatcaaa tgatcgcgcc cagcaaaagc ttgatcaaac
     2521 gtttgataag ctgcggtcaa tgctgccttg gaataaacgt agcagggtgt gccaaattcc
     2581 tgagcaatcg ctgataatgc gactgactca atctgcagtt ggtcgctcag ataatcaaaa
     2641 tacgcaaaac cgctcatggc ttgacttgat cttctggcgt atcttttatt ttaggtggcg
     2701 gttcatcagg caagtaaagc ggtcctttca gcccgcatgc gattaacccg atggtcaaca
     2761 acacgattag acaatagcga tgcacgtgaa taatcaataa tgaaaaagaa gcaatattaa
     2821 cacatgcaaa cgaattacaa cggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnngatcaaa cacttgcgtg aattcttcga gctcaatatg caaggcatag tcagatttaa
     3001 gcccttcttg actactaatg acccctttgc gggtcagttg tgataaatcg tcttgaatgc
     3061 ggcgcgtcaa cagttttgcc ggcgcagtaa cccagcgact atgggcataa tgataagtcc
     3121 gcgctggatc gtgatacgcc aagcggtatt gcatagcagg gcttgtcagc caaatgggtg
     3181 cagtgatttc agtcatcatg atcgatacat cgaccggggc aacttgaact tcggaaaccg
     3241 gctttagacc cagatcatac aaggccagtg atgtgggttt attgctggaa aacgaacaac
     3301 caaccagcag actcaaacca ataataagaa ttttcatgaa aacgatttac ggtgaatgaa
     3361 accccgtttc accgggccct ggtgttgcaa tgggcgcgcc gaacaaaagg ctttgtgggt
     3421 gctcttcaag tctttgcagt aaccgattga tatgatgcgt actctgcgtg atcgactcag
     3481 ttgcctcacg taatttaggt aatgaatcag caaatgcctg cacactttgc gtcaaaccat
     3541 cgctcaaccc ccccggttgg ttggtcttaa caattgtttg gttgacttca gccagcaatt
     3601 gatgcgtttg cttcatgacg cgattggctt ccgcggatag cgtaggcaaa gattttaatc
     3661 caggctgcag ttgttcaacc aaacttccgt agcgttgaat cgcttgcgcc aaattagcta
     3721 gaatagcgct gaattgctcc tgattcttct gattcatcaa cgcattgagg cgtttcacag
     3781 ctttattcgt attgttcaaa atattttcaa ccgaatcgga aagtttgcgg ataaacgagg
     3841 cacgcagctc aatacgcgca ttggatggca aataagtctc ttctggatca ccttcagtat
     3901 tcagttcaat gtacgcaagc cccgtcaatc cctgattagc taattgtgca taaatatttg
     3961 ttggcaattt aatgtcgctg ttcacggcaa tgccaacgag aatgaaggtt tggtcctgag
     4021 tatcaaaccg aatttcctcg actttaccaa tcgatactcc tcggtaacgt acaacggctg
     4081 ctggattcag gccggatacc gaccctgcct ccgataccaa taaataacga tcacgcagaa
     4141 tatcttggtt gctaaaccaa cgaaccgcat aacccgtcgc aactccaaga ataatcacga
     4201 agattccggc tgcgagtgca tgagcacgat tttccatttg ctaatttaaa ttgaggtaga
     4261 tcaaattaat tgggtcgaga tacggtcgag cgcgaattca gatgccaata cggaaatcat
     4321 tttaattaca ataagatttg atagtttatc agctaatggc tttaatccag tttattctta
     4381 agatcggtcg ctttatcttt taacgtatcc caggtttctt ctgtgcggtt tttcatcttg
     4441 cgcttcaaac attcggcttc actttcagtg cacaacgctt cttcagcacg gtttaccgtt
     4501 tttttggctt cgcgcttcaa ttctttcgat gtggcttcta ctttttccgg aacggtttcg
     4561 gttgcatgta ctgttgctag ggttaaccct agtgtaatta aaaccacata gaaaacgagc
     4621 ttcatcatag atctccaatt aatagattga gtaagaatac tgctgtacat taagcaaact
     4681 gct
//
LOCUS       DAOQKQ010000190         4630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_10426_length_4630_cov_6.071694, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000190 DAOQKQ010000000
VERSION     DAOQKQ010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10426_length_4630_cov_6.071694"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..1352
                     /gene="sucC"
                     /locus_tag="PKZ49_10890"
     CDS             183..1352
                     /gene="sucC"
                     /locus_tag="PKZ49_10890"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HND37112.1"
                     /translation="MKIHEYQAKEILRQFGVTTPNGIACFSVNEAISAAQSLGGNRWV
                     VKAQIYAGGRGKGGGVKLARSLDEVRQFAEGMLNKPLITHQTGPQGQIVHRLYIEQGV
                     DIQHEYYLGMVIDRARQRVCLMASAEGGMDIEEVAAKTPEKIHKILIDPVAGLKDEDI
                     THIAGKISLPSTSLDATRVLLKGLYQAFDQTDASLLEINPLILTTSGQVIALDAKMNF
                     DDNALFRHPEIVALRDTDEEDPAEMEAAKHDLSYIALDGNIGCLVNGAGLAMATMDII
                     KLYGGHPANFLDVGGGATAEKVTEAFKIMLRNPKLQAILVNIFGGIMKCDVIAQGVTT
                     AAREVKLTVPLVVRLEGTNVDLGKKILAESGLPIISASNMADAAEKVVNAIQKGA"
     gene            1355..2230
                     /gene="sucD"
                     /locus_tag="PKZ49_10895"
     CDS             1355..2230
                     /gene="sucD"
                     /locus_tag="PKZ49_10895"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HND37113.1"
                     /translation="MSILINRDSRVITQGITGKTGQFHTQLCREYANGRQCFVAGVNP
                     KKPGEQFEGIPIFATVKEAKQATGATVSVIYVPPPFAAAAIDEAVEADLDLVICITEG
                     IPVRDMLRTRYKTQGKKTLLIGPNCPGIITPDEIKIGIMPGYIHKKGRIGVVSRSGTL
                     TYEAVAQLMALGLGQSTCIGIGGDPINGLKHLDVLRLFNDDDETDAVLMVGEIGGTDE
                     EDCARWVKDHMKKPVVGFIAGVTAPPGKRMGHAGAIIAGGKGTAQEKIEVMEACGIRV
                     TCNPAEMGKLLKSVL"
     gene            2247..2627
                     /locus_tag="PKZ49_10900"
     CDS             2247..2627
                     /locus_tag="PKZ49_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37114.1"
                     /translation="MMESVQVFWSRKPYFLSSILLIIFLTSCATTNQLPLPPSKPSTK
                     PSSPVTQPKQAPRPAGPLPPSTRPTYNLSGFPPATKEGYIDGCETAKKTRWGYKDDVR
                     YSNDLQYRMGWDDGHTICGNQPTK"
     gene            2587..4137
                     /gene="dacB"
                     /locus_tag="PKZ49_10905"
     CDS             2587..4137
                     /gene="dacB"
                     /locus_tag="PKZ49_10905"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HND37115.1"
                     /translation="MMAIPFVAINQRNSRPGIAIVMTMLSDKRINVRHSIKLNLQAFF
                     LIGILSLLSLSLSASELPESVRHALKTAGIPETAVGVYVKEVTVKQPMLTMNPTQALN
                     PASVMKLVTTYAGLELLGPAFNWKTEFYALGPIEGGVLKGDLAIKGYGDPQLNLENFW
                     LLVRKLRQTGLKEITGNLILDNSYYQFSMGDPGAFDGSPLKTYNVFPEALLVNYRTTA
                     FHITPDPDRNSVRIVTDPISELLDLQNNLKLTRGHCGDWRNQMRVDIRKPEGNGGRVT
                     ATLSGNYSIQCGANIYYMSLHDSAAYIHDFFKKLWIQQGGIFKGSVQQGIVPKNISPL
                     TVYQSPPLAEIIRGINKFSNNVAARQLYLALGQSLGQSGNAEFVSLDQSNIALRQWLF
                     SKRLSFPELVVENGSGLSRRERISAYHMGELLVAAFHSPVMPEFMASLAISSVDGTMR
                     SRLRGSLVAGKAHMKTGTLNDVSAVAGYVLDHKDRRSVVVFLVNHPNASNARSAMDAL
                     IRWVYEKP"
     gene            4185..4616
                     /locus_tag="PKZ49_10910"
     CDS             4185..4616
                     /locus_tag="PKZ49_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37116.1"
                     /translation="MRIISFHSVIYGLLVGSVLVIANPVSATKMAGTFVSQQTCPAYV
                     SKKKLTNPDESQLTVAGKYTIIEANQKNDPSWYRVIVPNARPKERWIKAECGVFHQEG
                     NGTGGGSAPNQCSTAGHADSYVLALSWQPAFCEIKIEQTRM"
BASE COUNT         1287 a          947 c         1193 g         1203 t
ORIGIN      
        1 cctcgtcgcg atcagtagga gcccaagtac tgggtgagtg gtgcgaaaag attgaacagg
       61 ctgctaggaa tcatgatgag cagagcctca ccagcctgtt gccgcagttt gatgctgaac
      121 tgcaagcggt taaacactat cttgaaacaa tgaacgcata atcctttgaa aggagttagt
      181 cgatgaaaat tcatgaatac caggccaaag aaattctacg tcaatttggg gtaactacgc
      241 ctaatggtat tgcctgtttc agcgtgaatg aagctatctc tgctgcgcag tcattgggcg
      301 gaaatcgatg ggtggtgaaa gcacaaatct atgccggtgg acggggaaaa gggggcgggg
      361 ttaagttagc tcgttcactc gatgaagtta ggcagtttgc agaaggcatg ctcaataagc
      421 cactgataac tcatcagact ggcccacaag ggcaaatcgt gcatcggttg tatatcgaac
      481 agggtgtaga tattcagcat gagtattatt tgggtatggt gatcgatcgt gcccgtcaac
      541 gcgtttgctt gatggcgagt gctgaaggtg ggatggatat cgaggaggtc gcagccaaaa
      601 cacctgaaaa aatccataag attttgattg atcctgtggc tggattgaag gatgaggata
      661 tcacccatat tgcagggaaa attagtttgc cgagtaccag ccttgatgca accagagtcc
      721 tgctaaaagg gctttatcag gcgtttgatc aaaccgatgc ttcgttattg gaaatcaacc
      781 cattaattct gacgacttca ggtcaggtaa tagcgctcga tgcaaaaatg aattttgatg
      841 ataacgcatt atttcgtcac cctgagattg tagcgttgcg cgatacggat gaagaagatc
      901 ctgccgaaat ggaagcggcc aagcatgacc tctcctatat cgctttggac ggaaacattg
      961 gttgcttagt aaacggtgct ggtttagcga tggcgaccat ggatatcatc aaattatatg
     1021 gaggacatcc agccaatttc ttggatgttg gtggtggtgc aacggctgag aaagtcacgg
     1081 aagcgttcaa gatcatgctg cgtaatccca agttgcaagc gatattagtc aatatctttg
     1141 gcggaatcat gaaatgtgat gtgatcgccc aaggggttac taccgcagcc cgcgaagtca
     1201 aattaacggt accgttggtg gttcggcttg aaggtacgaa tgttgatctt ggtaagaaaa
     1261 ttctcgccga atcgggctta ccgatcattt ctgccagtaa tatggccgat gcagccgaga
     1321 aagtggttaa cgcaatacag aaaggagcct agccatgtcg atactgatca atcgtgacag
     1381 tcgagtgatt acacagggca ttaccggaaa aaccgggcag tttcatacgc agctatgccg
     1441 tgaatatgct aatggacgcc agtgctttgt cgctggggta aatcctaaaa agccaggcga
     1501 gcagtttgaa ggcattccta tttttgctac tgtcaaagaa gcaaaacaag caactggtgc
     1561 cacagtatcg gtgatttatg tcccgcctcc ttttgcggct gcagccattg atgaggctgt
     1621 tgaggctgat ctagatttgg tgatctgtat tactgaaggt attccggtgc gtgatatgct
     1681 gagaacccgc tataaaacgc aagggaagaa aaccttgctc atcggcccga attgcccagg
     1741 gattattacg ccggatgaaa tcaagattgg tattatgccg ggctatattc ataaaaaggg
     1801 gcgaatcggt gttgtttccc gttcggggac gctaacgtat gaagcagtag cccaactcat
     1861 ggccctgggc ctgggtcaat cgacctgtat cggtatcggg ggagatccca tcaatggatt
     1921 gaagcatctc gatgtattga ggctatttaa cgacgatgat gaaacggatg ccgtgttaat
     1981 ggttggtgaa attggtggta ctgatgaaga ggattgtgca cgctgggtca aagatcatat
     2041 gaagaaaccg gtggttggtt tcattgcggg tgtgaccgca ccgcccggta aacggatggg
     2101 gcatgcaggt gcaattattg caggtggtaa aggcacagcc caagaaaaga tcgaagtgat
     2161 ggaagcctgt ggtattcgtg ttacctgtaa tcctgcggag atgggtaaat tgcttaaatc
     2221 tgttttatag aggaacccgc attttgatga tggagtctgt gcaagtgttt tggagtagaa
     2281 agccttactt tctatcaagc attctactga taatcttttt gacgagctgc gcaactacga
     2341 atcagttacc tttgccaccg tctaagccga gcaccaaacc ttcatcaccc gttacccaac
     2401 ccaaacaggc acctcgcccg gcagggccat tgccgccaag cacgcgaccg acttataact
     2461 tgagtggctt tcccccagca actaaggaag gctacatcga tggttgtgaa acagcaaaaa
     2521 aaacacgctg gggatacaaa gatgacgtgc gttatagcaa tgatcttcaa taccggatgg
     2581 ggtgggatga tggccatacc atttgtggca atcaaccaac gaaatagtag accgggtatt
     2641 gcaatcgtga tgactatgct aagcgataag cgtatcaacg tgcggcactc catcaaattg
     2701 aacctgcaag ctttcttcct cattgggata ctaagcttgt tgtcgttatc gctgtcagct
     2761 tcagaattgc cagaatcagt gcggcatgcg ttgaaaactg ctgggatccc ggaaacagcg
     2821 gttggggttt atgtaaaaga agtaactgta aagcagccaa tgcttacaat gaatccgacg
     2881 caagcattga atccggcgtc tgtgatgaaa ttggtaacga cctatgccgg gcttgagttg
     2941 cttggtccag cttttaattg gaaaaccgag ttttatgcac tgggtccgat tgaaggcggt
     3001 gttctgaaag gggatctcgc gatcaagggt tatggagatc cacagcttaa tttggaaaat
     3061 ttctggttgt tggtgcgtaa attgcgccaa acaggtttaa aggaaataac cggaaactta
     3121 atcctcgaca atagctatta ccagttctcg atgggcgatc cgggtgcatt tgatggcagt
     3181 ccgttaaaaa cttataacgt atttcctgaa gcactattgg tcaattatcg tactactgct
     3241 tttcatatta cgcctgatcc cgatcgcaat agcgtacgta ttgtgaccga tcctatctcg
     3301 gaattactcg atttacagaa taatttgaaa ctgacgcgtg gccattgcgg ggattggcgt
     3361 aaccaaatgc gcgtcgatat tcgtaaacca gagggaaatg gggggcgtgt taccgctacg
     3421 ctgagcggta attattcaat tcagtgtggc gcgaatattt attatatgag tttgcatgat
     3481 agtgcggcgt atatccacga ttttttcaag aaactgtgga tccaacaagg tggaattttt
     3541 aaaggttcgg tgcagcaagg gatcgtacct aaaaatatat caccactcac ggtatatcaa
     3601 tcacccccat tggctgaaat tattcgtggt atcaataaat tcagcaataa tgttgcagca
     3661 agacaacttt atttagcgct cggacaatca ctggggcaat ccggtaatgc tgaattcgta
     3721 tcactggatc aatcaaacat tgcgcttaga caatggcttt tttccaaacg ccttagtttt
     3781 ccagagctcg tagtagaaaa tggctctggg ttgtcacgcc gagaacgtat tagtgcctac
     3841 catatgggag aactattggt ggcagcattt catagtccgg taatgcctga gtttatggca
     3901 tcactcgcga tatcatccgt ggatgggacg atgaggagcc gattacgagg ttcattggta
     3961 gcaggcaaag cacatatgaa aaccggtacc ttgaatgatg tatcagcggt tgcggggtat
     4021 gtactcgatc ataaagatcg tcgatccgtt gtagtattcc ttgtcaatca tcccaatgcc
     4081 agtaacgcac gatccgcgat ggatgcgcta atccgatggg tatatgaaaa accttaatcg
     4141 ctaaggaatc gtatatcgaa acttattatt agtggagtaa ctgcatgcga attatttcct
     4201 ttcatagcgt aatctacggt ttgttggtgg ggagtgtact ggttattgct aaccccgtat
     4261 cggctacaaa aatggcgggc acctttgtga gccaacaaac ctgtcctgcc tatgtttcta
     4321 aaaagaaact gaccaatcct gatgaaagcc agttaactgt agctggtaaa tatacgatta
     4381 tcgaagctaa tcaaaaaaat gatccaagct ggtaccgggt aatcgtaccg aatgcgcgcc
     4441 ctaaagaacg ttggatcaaa gcagaatgtg gtgtttttca tcaggaagga aatggaacag
     4501 gtgggggaag tgctccaaat caatgtagca cggcagggca tgcagatagc tacgtgctcg
     4561 cattatcctg gcagccagca ttttgtgaaa tcaaaatcga acaaacccga atgtagtatt
     4621 accgatccta
//
LOCUS       DAOQKQ010000191         4557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_10718_length_4557_cov_5.313861, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000191 DAOQKQ010000000
VERSION     DAOQKQ010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4557
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10718_length_4557_cov_5.313861"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..620
                     /locus_tag="PKZ49_10915"
     CDS             153..620
                     /locus_tag="PKZ49_10915"
                     /inference="COORDINATES: protein motif:HMM:NF012280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kazal-type serine protease inhibitor family
                     protein"
                     /protein_id="HND37117.1"
                     /translation="MQKLMSVAILGAILLMQACTNNEPPEPSPLPVACGGIQGKQCPN
                     ANQYCNLGMGNCSIADAQGTCADMPTICPELYQPVCGCDGKTYGNTCKAAAAGVSIDH
                     EGECESTPQACGGVAGLQCPQGQVCKDDPRDSCDPEKNGADCPGICEAPHVSQ"
     gene            632..1675
                     /locus_tag="PKZ49_10920"
     CDS             632..1675
                     /locus_tag="PKZ49_10920"
                     /inference="COORDINATES: protein motif:HMM:NF013890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37118.1"
                     /translation="MKSFWLIGLVLLTASCTHFAIEDCGSQVLGVNEARIRSSHFRML
                     LEDSSAAANRFLPTAVMSTLAYAEDRDCGKETAKITNQERESLEKILQTERWIEVTEI
                     EWVPACEDDVGLFYRVWKKEFDDTIQVVVAFRGTWELKDWFYGNMHWLTRFLPIEDQY
                     SSARKNMQKVFDYFGESKKPVQFFTTGHSLGGGLAQHILYSNPTKIIQAIAFDPSSVT
                     GFTDQTPEDQVSACECDSSVLNGETRIYRVYDAYEILANLRIFHKFFFPPERHIQEVR
                     FPNEASHSMKGLAFYLFHRANDKGTDIYANPWYSGKGNYDGTRSCTDAFANKQKKSCS
                     IKVTTDDWFKCPQ"
     gene            complement(1733..2197)
                     /locus_tag="PKZ49_10925"
     CDS             complement(1733..2197)
                     /locus_tag="PKZ49_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HND37119.1"
                     /translation="MHYFVILIIASCIVSICVILHYEALRFLGSTLGAHVHKRIGVLL
                     VMFGLLIAHILEIWIFAIGYILVQHGIELGHIQGIEQGNLIDFVYYSSVVYTTVGFGD
                     LVPVGAIRMLTAAEGLTGLALITWSASFTFMAMQRFWPHPLANSHKKPGERD"
     gene            complement(2380..2724)
                     /gene="smbP"
                     /locus_tag="PKZ49_10930"
     CDS             complement(2380..2724)
                     /gene="smbP"
                     /locus_tag="PKZ49_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small metal-binding protein SmbP"
                     /protein_id="HND37120.1"
                     /translation="MKLIRNSIAMGVLAMFLSAPLLAGDAAHTSAAMEHAGEAKAHGE
                     MGHAKELLKHAKESLAHAKAAGKEHADAHKHMDEAIKHLEEAIKHAEMDHAGEAAKHT
                     EEAMKHMRESGH"
     gene            2949..4550
                     /locus_tag="PKZ49_10935"
     CDS             2949..4550
                     /locus_tag="PKZ49_10935"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HND37121.1"
                     /translation="MTEEQRRQLQQQLWNIANTLRGKMNADEFRDYILGFIFYKYLSE
                     KVERFADSILEQDQLKFAAIDEQTAEGAEIVQAVHDAAVTELGYFLKPSELFHAIAMK
                     GNSQADSDADGEHYNFILPDLTRILKNIEQSTLGTDSADDFSNLFEDLDLTSSKLGAT
                     EKAKNALVTKVLVHLDKIDFSLADGTADVLGDAYEYLIGEFASGAGKKAGEFYTPQPV
                     STLLAKLVTAQNKNIKKLYDPTCGSGSLLLRVKREADHVDKIYGQELNRTTYNLARMN
                     MILHDVHYADFDIRQEDTLEHPQHRELRFDAIVANPPFSAHWSANPLFMSDDRFSVYG
                     KLAPSSKADMAFVQHMVYQLAEDGTMAVVLPHGVLFRGAAEGHIRQYLIAQTNCLDAV
                     IGLPANIFYGTSIPTCVLVFKKCRRNPDNILFIDASQAFDKVKTQNLLRPQHIEKIVE
                     AYLARSDQAKFSHVASLETIKANDYNLNIPRYVDTFEAEAEIDLNAIAEQLQALEIQS
                     QQTDATIAAYCRELGIAPPFAESGK"
BASE COUNT         1250 a         1066 c         1090 g         1151 t
ORIGIN      
        1 atctcttcct cctcctctca aatcatttta tctccggaaa acccggggcg attcaaattg
       61 aaaggtatct tttgtatgac tgagcttgct tctgctggct ggtctgcaaa tcacatttaa
      121 ttcgcagtaa gataacttga aaggagtcaa aaatgcagaa actcatgagt gtcgctattt
      181 tgggcgctat cctgctgatg caagcttgta ctaacaacga accacctgaa ccctcgccac
      241 ttcctgtggc atgtggtggc atccagggaa aacaatgccc caacgcaaat cagtattgta
      301 atttagggat gggtaattgc agcatagcgg atgcacaagg aacctgtgct gatatgccta
      361 ctatttgccc agaactctat caaccagtct gtggctgtga tggaaaaacc tatggtaata
      421 cttgcaaagc tgcagcggca ggtgtatcca ttgatcacga gggtgaatgt gagtcaacac
      481 cacaggcttg cgggggagtt gctgggctgc aatgtcctca aggtcaggtc tgtaaagatg
      541 atccgaggga tagttgcgat ccggagaaaa atggcgcaga ttgccctggg atatgcgaag
      601 cacctcacgt cagtcaatag cgggttaaca aatgaaatct ttctggttga ttggtttagt
      661 tctgcttaca gcatcctgta ctcattttgc gattgaggac tgtggaagcc aagtgctagg
      721 cgtcaatgag gcgcggattc gctcatcgca tttcaggatg ttgcttgaag actcatctgc
      781 tgcagcgaat cggtttcttc ccaccgcagt gatgtcaacc ttggcttatg cagaagatcg
      841 tgattgtgga aaagaaaccg caaagattac caatcaagag agagaatcgc ttgaaaaaat
      901 cttacagact gaacgctgga tcgaagtaac cgagatcgaa tgggttccag cttgtgagga
      961 cgatgttggt ttgttttacc gcgtgtggaa aaaagaattc gatgacacga tacaagtcgt
     1021 cgtggctttt cgcggtacat gggagcttaa agactggttt tatggcaaca tgcattggtt
     1081 gacacggttc ttaccgatcg aagatcaata ttcttctgcc cggaaaaata tgcagaaagt
     1141 tttcgattat tttggtgaat cgaagaagcc agtgcagttt tttacgacag gccattctct
     1201 cgggggtggt cttgctcagc acatactgta ttccaatcca accaaaatca tccaagcaat
     1261 cgcattcgat ccgagttcag tgactggttt tacagatcaa acaccggaag atcaagtttc
     1321 agcgtgtgaa tgcgattctt cagtgttgaa tggagaaact cgtatttatc gggtctatga
     1381 tgcctacgaa atattagcga atctgcgcat tttccataaa tttttctttc cacccgaacg
     1441 ccatatacaa gaagtgcgct ttcccaatga agcatcccat tccatgaagg gtttggcatt
     1501 ttatttattt catcgagcaa acgacaaagg cactgatata tatgctaatc cctggtattc
     1561 agggaagggt aattatgatg gaactcgatc ttgcaccgat gcttttgcca ataaacaaaa
     1621 aaagagctgt agcatcaaag tgactaccga tgattggttt aaatgtccgc agtagaaaca
     1681 attttcataa caattgggct ggactgttgt tttctgatag gtctgcgtta gattaatccc
     1741 gttctcctgg cttcttgtgc gaattcgcca ggggatgcgg ccagaagcgt tgcatggcca
     1801 tgaatgtaaa cgaagccgac caggtgatca acgccaggcc ggtcaatcct tctgcagccg
     1861 ttaacatgcg aatcgcgccc acggggacca aatcaccgaa tccaaccgtg gtataaacga
     1921 ccgacgagta atacacaaaa tcgatgagat taccctgttc aatgccttgg atatggccca
     1981 attcaatgcc atgctgcact aggatataac caatcgcgaa aatccaaatt tcgaggatat
     2041 gggcaatcag caatccaaac atcaccagca acacaccaat acgcttatga acatgtgcgc
     2101 ccagggttga acccaagaag cgcagcgctt catagtgcaa gatgacgcaa atcgaaacga
     2161 tgcagctcgc gatgatcaaa ataacgaaat agtgcatagg ggtcggttaa gtcagaaata
     2221 agccaatttt acagggacta cccattgaat agacaagtca attaatcaga aactaagttt
     2281 ctgaattgat gaggagattg tgcaggaaaa aggaaaatct ccctcagcgt tagagcagtg
     2341 tggttgctga gggagtgggt ttataaaacg gtgtggagat tagtggccgg attcacgcat
     2401 atgtttcatg gcttcttcgg tatgtttggc tgcttcaccc gcatggtcca tttcggcatg
     2461 tttgatcgcc tcttccaaat gcttgatggc ttcatccata tgtttatgcg catccgcatg
     2521 ttctttgcca gccgctttgg cgtgcgccaa actttctttg gcatgtttca acagctcttt
     2581 ggcgtgcccc atttccccat gcgccttcgc ttctcccgca tgttccatag ctgcagatgt
     2641 atgcgcggca tcgcctgcta acagcggcgc gctgagaaac atagccaaca cacccattgc
     2701 gatactattt cgtatcaatt tcatcgaaaa ctcctttaac ggttacatga ttgatgacta
     2761 ctgacattca ttcttattga cattgattta ctcaactcag ttccatgcta gatattttta
     2821 aagtgcatcg caataagtgc taacccttat agacgatggt ggttcaagca gatacactgg
     2881 tctatttagc atgtcagcat cggtcgattg gcagtacact atagccattt gaaattacta
     2941 ccagtcacat gaccgaagaa cagcgccgac aattgcagca acagctgtgg aacatcgcca
     3001 ataccctgcg tggcaagatg aatgccgatg aatttcgtga ttacatcctg gggttcattt
     3061 tttataaata cctgtctgag aaagtggagc gttttgcgga ttccatcctg gaacaagatc
     3121 agttgaaatt cgcggccatc gatgaacaaa ccgctgaagg cgcagaaatc gtgcaagcgg
     3181 tgcacgatgc cgcagtgacg gaactgggtt atttcctcaa gccgtctgaa ttgttccacg
     3241 ccattgcgat gaaaggcaac agccaggccg atagcgatgc ggatggcgaa cactacaatt
     3301 tcattttgcc cgatctgacg cgtatcctga aaaatatcga gcaaagcaca ctcggcaccg
     3361 acagtgctga cgattttagc aatctgtttg aagacctgga tttaacctcg tccaaactgg
     3421 gtgcaaccga gaaagctaaa aatgcactgg tgaccaaagt gctggtgcat ttggacaaaa
     3481 tcgatttcag cttagccgat ggtacggcag acgtgctggg cgatgcctat gaatatctga
     3541 tcggtgaatt cgccagcggc gcgggcaaaa aagcagggga attctacacg ccgcagccgg
     3601 tttccacctt gctggccaaa ctggtaaccg cgcaaaataa gaacatcaaa aaactgtacg
     3661 atccaacctg cggcagtggt tcattattat tgcgcgtcaa acgcgaagcc gatcacgtcg
     3721 acaagattta cggccaggaa ttgaaccgca ccacgtataa cctggcacgc atgaacatga
     3781 ttttgcacga cgtgcattac gccgatttcg acatccgtca ggaagataca ctggaacacc
     3841 cgcagcatcg cgagctgcgc tttgatgcga ttgttgccaa tccaccgttt tccgcgcatt
     3901 ggagcgccaa tccgctgttt atgagcgatg atcgctttag tgtctatggc aagctagcac
     3961 ccagcagcaa agccgatatg gcgttcgtgc aacacatggt ttaccaactt gcggaagatg
     4021 gcaccatggc cgtggtgttg ccgcatggcg tgctattccg tggcgcagcg gaaggccata
     4081 ttcgccaata tttgatcgca caaacgaact gcctggatgc ggtgatcggt ctaccggcca
     4141 atattttcta cggcactagt attcccacct gcgtgctggt tttcaaaaaa tgccgccgca
     4201 acccggacaa tattctgttt attgacgcca gtcaggcatt cgataaagtt aaaacgcaga
     4261 acctgctgcg cccgcagcat atcgagaaaa ttgtcgaggc gtatctagcg cgtagcgatc
     4321 aggctaaatt cagtcatgtc gcgagcctgg aaaccatcaa agccaatgat tacaatctca
     4381 acattccgcg ctatgtcgat acctttgaag cagaagcgga aatcgatctg aacgccattg
     4441 ccgagcaact gcaagcgctg gaaatacaaa gccagcaaac cgatgccacc attgcggcgt
     4501 attgccggga attgggcatt gcgccaccgt ttgcggaatc agggaaataa gcatgaa
//
LOCUS       DAOQKQ010000192         4544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_10759_length_4544_cov_6.001782, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000192 DAOQKQ010000000
VERSION     DAOQKQ010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10759_length_4544_cov_6.001782"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="PKZ49_10940"
     CDS             complement(<1..576)
                     /locus_tag="PKZ49_10940"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HND37122.1"
                     /translation="MKKIRILLVALLMFFSPIGSVWSLPFTSMYVFGDSLSDGGSSPS
                     AVLSIYKLLGGDCDPFHPCPPYFEGRYSNGAVAAEYLAQQILPGGTSTSHFFNYAVSG
                     ATTGIGNFGDNGTTTTPGSFGLPGMNQEIGLYLSNVNGVADSQALYFIWGGANDFLTG
                     NAPDQAAQNIANYVSTLISAGAESFFIPNLPD"
     gene            complement(1029..3743)
                     /gene="acnA"
                     /locus_tag="PKZ49_10945"
     CDS             complement(1029..3743)
                     /gene="acnA"
                     /locus_tag="PKZ49_10945"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HND37123.1"
                     /translation="MNHNLFNSLKTLQLPGKSHQYYALSALEEAGLGNISRLPISIRI
                     VLESVLRNFDDKKITESHIKQLAGWEPNAARIFEIPFVVARIVLQDFTGVPLLVDLAA
                     MRSAAMRLGKDPNRIEPLVPVDLVVDHSVQVDYYGDKQALARNMEIEFERNKERYQFI
                     KWGMQAFDTFKVVPPGIGIVHQVNLEYLARGVHLKQGLIYPDTLVGTDSHTTMINSIG
                     VVGWGVGGIEAEAGMLGQPVYFLTPDVVGVNLTGQLREGVTATDLVLTITEMLRKAKV
                     VGKFVEFYGEGTASLSLPDRATIANMAPEYGATMGFFPVDDATIEYFSHTGRTAEEIQ
                     AFTSYFKAQGLYGIPQQGDIDYSQELHLDLGTIVPSLAGPKRPQDRIPINQLKTTFST
                     LFSKSITENGYGKSPELLAQGFPTIHKALNQPVELHHGDVLIAAITSCTNTSNPSVLI
                     AAGLLAKKAIEKGLSVKRHIKTSLAPGSRVVTAYLTAAGLLPYLERLGFNIAGYGCTT
                     CIGNSGPLPESIEESIIKEDLICSAVLSGNRNFEARVHANIRANFLASPPLVVAYAIA
                     GTVLKDLTTEPIGIGNGNQPVWLRDIWPTSAEIHELMKYAMQADTFRELYRDLTKDHT
                     LWNNITAVSGKLYDWPESTYIAEPPFFQDFSLQPTQTGRSAEITGARALAFFGDSVTT
                     DHISPAGAIKDTSPAGKYLLDHHVTKADFNSYGSRRGNHHIMMRGTFANVRIKNLMIP
                     GSEGGITLFRQAKSNECQQMSIYDAAMRYMEQATPTLLFAGKEYGTGSSRDWAAKGTQ
                     LLGVKAVIAESYERIHRSNLVGMGVLPLQLKENESVQTLSITGDEFFDIKGIETIQPQ
                     QTVTLVIHRPNGEQSQVQLICRIDTAIEVEYYYQGGILPYVLRQLLNH"
     gene            3853..>4544
                     /locus_tag="PKZ49_10950"
     CDS             3853..>4544
                     /locus_tag="PKZ49_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HND37124.1"
                     /translation="MNITQLNQNFGIANTVKFIEGQGGLPFIQIHTDKAQALISVYAG
                     QVLSFKPVSQEADLMFLSSKAYYQAGKAIKGGVPICWPWFGADPEGKGRPAHGFVRNR
                     MWDVIETDLNSKGEVKVTLGLNDTPETQAIWPYSFQLRLVITISETLGLELITHNTGN
                     QAFTITQAFHTYFGVKDIHRATVTGLDGKSYLDKVDAGQQKQQSGDVKISSEVDRIYL
                     DVSNELTIHDAA"
BASE COUNT         1305 a          950 c          990 g         1299 t
ORIGIN      
        1 atctggaaga ttggggataa agaaagattc agcgccagcc gagattaatg tactcacgta
       61 attcgcaata ttttgggctg cttgatcagg ggcattacca gttaaaaaat cgttggcacc
      121 tccccagata aaatataaag cttgtgaatc agctactcca tttacattcg agagatatag
      181 cccaatttcc tgattcatgc caggtaaccc aaacgaacct ggtgtcgttg tcgtgccatt
      241 gtcaccaaaa ttaccaatcc ctgtcgttgc gcctgaaacg gcataattaa agaaatgact
      301 tgttgaagta ccaccgggaa gaatttgttg tgcgagatat tcagccgcaa ctgcgccatt
      361 ggagtatcgt ccctcgaaat aaggagggca tggatggaaa gggtcacaat ctccacctaa
      421 tagcttataa atacttaaca cggctgaagg actacttcct ccatccgaaa ggctatcacc
      481 gaagacatac atgcttgtga agggtagaga ccaaacactt cctatgggtg aaaaaaacat
      541 caacaaagca accagcagaa ttcgtatttt tttcatattg agctttggtt aacgataaat
      601 ataaatacca ttttttctga agactcagta tgcaatatga atcgttaatt cgcaagaaaa
      661 aatataaccc actgattatg cggtagttaa gtgatttttc tcacagtcag ttagattttt
      721 attcgataaa ctgttgacaa tttggtgcag attgcacgat gatgtagcaa gccatgaatt
      781 tcatgggcgg ccattttaaa atacattaat agaggtaatt aacttttgat tgatttgaaa
      841 aaaaccgtac caatgagtaa agaggacttt tgtttaataa tgctatttcc ttcctcctca
      901 aaggcatctt aacagtttaa gcaattagcc tgactttaag atgcctcttt tttttacctc
      961 cccctgttta aattttactc agtagtattt cttataaatt aacaacgatt ttgttgttct
     1021 agcttgcgct aatgattgag taactgcctg agtacataag gtaggatacc gccttggtag
     1081 taatattcca cctctatcgc tgtatcgatt cgacaaatta attgaacttg gctttgctcg
     1141 ccgtttggtc ggtggatgac caatgtaacc gtttgttgag gttgaatcgt ttcaatgcct
     1201 ttaatatcaa agaattcatc tcccgtaatg ctaagcgttt ggacgctctc attttcctta
     1261 agttgcaaag ggagtacgcc catacccacg aggttgctgc gatgaatacg ttcatagctt
     1321 tctgcaataa ctgctttaac accgagtaat tgggtgcctt tagctgccca atcgcgactg
     1381 gatcctgtgc catattcctt accagcaaat aggagcgtag gggttgcttg ctccatatag
     1441 cgcatggccg catcgtatat tgacatttgc tgacactcat tcgacttcgc ctgtctaaat
     1501 aaggtaatac caccttcgct acccggtatc atcaaatttt taattcttac attggcaaat
     1561 gtgccgcgca tcataatgtg gtggttacca cggcgtgagc cgtagctatt aaaatcagct
     1621 ttggtaacat ggtgatctaa tagatactta cctgcaggcg aagtatcttt aatcgcgcca
     1681 gcagggctga tgtgatcggt tgtcacggag tcaccaaaga atgccagtgc gcgtgcacct
     1741 gttatttcag cagaacgacc ggtttgagtc ggttggagcg agaaatcttg gaaaaaaggc
     1801 ggctcggcga tataggtcga ttcgggccag tcgtataact ttccggacac agcagtaatg
     1861 ttgttccaga gtgtgtgatc ttttgttaaa tcacgataga gttcacggaa agtatccgct
     1921 tgcatggcat atttcattaa ttcatgaatt tcagcactcg ttggccaaat atcacgtagc
     1981 cagacaggtt gattaccatt accgataccg atcggctcag tggtaaggtc ttttaatacg
     2041 gtacctgcga ttgcataagc gacaaccaat gggggagaag ctagaaaatt agcgcgtata
     2101 ttggcatgga ctcgcgcttc aaaatttcga tttccagata aaacagccga gcagattaaa
     2161 tcttctttga tgatgctttc ttcaatcgat tcagggagtg ggccagaatt gccaatacaa
     2221 gtcgtacacc cgtagcctgc gatattaaag cctagtcgtt ccaaataagg aagtaaccca
     2281 gcagctgtaa gataagcagt gactactctt gatccgggcg cgagtgaagt tttaatgtgg
     2341 cgcttaactg ataatccttt ctcaatggct tttttagcga gtaatcctgc agcaatcagt
     2401 acgctaggat ttgaagtatt agtacacgag gtaattgcag caataagaac atcgccatga
     2461 tgcaactcaa ccggttgatt caacgcttta tgaattgtgg ggaagccctg ggccagtagt
     2521 tcaggggatt taccataacc attttcagta atcgatttac taaagagtgt tgagaaagtg
     2581 gttttgagtt gattaatggg aatgcgatcc tgaggtcgct taggcccagc caatgaaggt
     2641 acaatggtgc ctaaatctaa atgaagttcc tggctataat cgatatctcc ttgttggggg
     2701 atgccataga gtccttgggc tttgaaataa cttgtaaaag cttgaatttc ttcggcagtt
     2761 cgcccggtat gactgaaata ctcgatggtt gcatcatcaa cagggaaaaa acccatcgtt
     2821 gcaccatatt ctggtgccat gttggcaatc gttgctcggt ctggcaaact taatgaagcg
     2881 gtaccttctc cataaaattc gacaaactta ccaacgactt tggctttgcg aagcatttcc
     2941 gttatggtga gtactaaatc agttgcggtc acaccttcac gtaactgccc tgtcaagttg
     3001 accccaacga catcgggggt caggaaatag acgggttgac ccagcattcc agcttctgcc
     3061 tcaatacccc cgactcccca gcctaccaca ccaatactgt tgatcatcgt cgtgtgtgaa
     3121 tctgtaccaa ctaatgtatc tggatagatt aaaccttgct ttagatgaac tcctctcgca
     3181 aggtattcca gattgacttg atgcacgatg ccaatacccg gcggaactac tttgaaagta
     3241 tcaaacgctt gcatacccca tttgatgaat tgataacgct ccttattgcg ctcgaattcg
     3301 atttccatat tacgagcaag cgcttgctta tcaccgtaat aatcaacctg aacggaatgg
     3361 tcaaccacta aatcaactgg aaccagtggt tcgatccgat tgggatcttt gcctaatcgc
     3421 atggcggcac tgcgcatcgc agccaaatca actaacagcg gtacacctgt aaaatcttgt
     3481 aaaacaatgc gtgcgactac gaatggaatc tcaaaaatgc gtgctgcgtt gggttcccat
     3541 ccagcgagtt gcttgatatg ggattccgta atttttttgt catcaaaatt tcgtagtacg
     3601 gattctaata cgatacgaat tgaaattggt aatcgcgaaa tgttacccaa tcccgcttct
     3661 tcaagggcag acagggcgta atattgatgc gatttaccgg gaagttgtag tgttttaagc
     3721 gaattaaaaa gattgtgatt cattcagtat gtacctcaaa gcatggcttt agtaatcaat
     3781 cctagtttat gatgtcttgt tttgagttta tcattccaat cctatttgct acaaaaaata
     3841 aggtaaaaaa agatgaatat cacgcaattg aatcagaatt ttggaatcgc aaacacagtt
     3901 aagtttattg aagggcaggg gggattaccg ttcattcaga ttcatacaga taaagctcaa
     3961 gcactcattt ctgtctatgc tggccaagta ctttccttta aaccggtgag tcaggaagca
     4021 gatttaatgt ttctgagtag caaagcttac taccaagctg ggaaagcgat taaaggggga
     4081 gttcctatat gctggccgtg gtttggtgct gatccggaag gaaaagggcg gcctgcccat
     4141 ggatttgttc ggaatcgaat gtgggacgtc atcgaaaccg accttaattc aaaaggtgaa
     4201 gttaaagtca cgcttggctt aaatgatacc cctgaaacac aggcaatctg gccctattca
     4261 tttcaactaa ggttagtgat tacaatcagc gaaacattgg gcttggagtt aattacccat
     4321 aataccggca atcaggcatt caccattacg caggcatttc acacttattt cggtgtgaag
     4381 gatattcatc gtgcgaccgt gactggacta gatggtaaat cctatctcga taaagtcgat
     4441 gcaggtcaac aaaaacaaca atcgggagat gtgaaaatca gttcggaggt cgatcggatt
     4501 tatttggatg tcagtaatga gctaactatt cacgatgccg cgat
//
LOCUS       DAOQKQ010000193         4514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_10879_length_4514_cov_5.322270, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000193 DAOQKQ010000000
VERSION     DAOQKQ010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10879_length_4514_cov_5.322270"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1418)
                     /locus_tag="PKZ49_10955"
     CDS             complement(84..1418)
                     /locus_tag="PKZ49_10955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative O-glycosylation ligase, exosortase A
                     system-associated"
                     /protein_id="HND37125.1"
                     /translation="MRDILVTIIFFGALPLVFKKPQIGVYLWAWIGYMNPHRLGWGFA
                     YNFPFGIIAAAAIILSLVIARKNITYFWSPIFSLLIVLNIWFLITTFFAIQPDISWTQ
                     WEKVIKIQLIALMIPWIINDKEKIHNFIWVIALSIGYYGIKGGVFTLTTGGSQHVLGP
                     AGSFISGNTEIGLALVSVFPLFWYLYLNTIQPWLRSGLVFAMILIPVAILGTQSRGAL
                     LAIVAIGFFLWLKSRKKLAPFIVIILFAPLLYNFMPDSWHQRMGTISSTGEERDSSAK
                     GRLLAWEFATKLALARPLGGGFECFTKENYLTYAPDIVAEGGRYQDAHSIYFELIGEH
                     GFIGLIIYLSLLVMGWKTASTIIKISKTSLSNKWAYDLASMIQVSIVGYAVGGAFLGL
                     SYFDLLYHYFVILAVLLKIVQQSVTENSQSSTTLDHEHLILKNAKITSKASI"
     gene            complement(1482..3218)
                     /locus_tag="PKZ49_10960"
     CDS             complement(1482..3218)
                     /locus_tag="PKZ49_10960"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HND37126.1"
                     /translation="MTIFAGILARYSNSSIPNSIIEALQGAISRHPDDKNALIEYKNN
                     NIYFAKIDIGALGELGHFTDSQLSGFIAGNPILQQNSGNVYSRLESLKLLASDISKDN
                     LEALRNCRGTYCAAIYDHENGKLFLVTDKIGVRPVYFWVHSDFVIFASALRILESLRH
                     HQKEIDLKGITEIACFGYPLSDRTPYKDIFSLYAGEIVCFDTNKIERKKYWDWNKLSS
                     KKAEDLHLAKQLHATFIESIKIRLKDQKIVAAFLSGGLDSRAIVAALRELNIDVLTAN
                     FAQQGSQDQVFGQLAADLLGTHHSHLPRGKTTIGDSYHKLSTINWINSKEFLSWNPIK
                     PRVIWSGDGGSMGLGHIYQNSEIIDAARKKDLTTAKQLFSSYNRWGIFPKLMKRSLAS
                     SAQQLLADGIQSELDSIHPEDLGRQFYLFLLLNDQRRHMVEHFENIDLDRIEFELPFF
                     DANFIAEIVQQPVDRFLRHIFYLDWLGQFDSKVLEIPWQAYPGHVPCPLPKPENLEYQ
                     WESTQEDKKQAIDLALQFADSFSQNQEFMTNYINKFYFELWKLLLQWGKSNQSYLIRF
                     PAVLYRYWVKVQ"
     gene            complement(3357..>4514)
                     /locus_tag="PKZ49_10965"
     CDS             complement(3357..>4514)
                     /locus_tag="PKZ49_10965"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HND37127.1"
                     /translation="PGAGYAIWWYPTDQSINPLSKSQLVTTWSYFPGLGHKLADELSI
                     LIWANERNWITNVGYWPYGHPLRLKAESWDGSNAPHLIDENKSSMRTARATKFAANDE
                     IFYIKMIRNGPENFVVQREVIQIEKNIWIVLDKFNNTIPRNTRTNWTFFPDLILQKGD
                     EINSFVATDVQKNKSMISVIKTSSNNEVNILSGNIDPFAGWVVIDETPMPANTITVHS
                     STEDDWQLAVFILDDSTRYPNIVIPTVSFFNLTHDDQWEATITNYRDINFSLSRTKEQ
                     IHLVTKDKLNHSESILNFIDIPYPKDEISAINQLVQFAYEKSTRRVPLISYRVKISYL
                     LLALFVLQEFCLLVARKYFIAITYYRVQLAILIFWLGAGAGLTEYYLVAGK"
BASE COUNT         1498 a          861 c          762 g         1393 t
ORIGIN      
        1 agccagagac agaccaccct gcaatccctg catcatcttg cgcgtgtgtt gcaaactttt
       61 caaacagttt gcgccacttt tcttcaaatt gatgccttac tggtaatttt ggcatttttt
      121 agaatcaaat gttcatggtc taatgtggtg cttgattgtg agttttccgt aactgattgc
      181 tgcactattt ttaataaaac tgccaatata acaaagtagt gataaagaag atcaaaataa
      241 gacaatccta aaaaggcacc gccaacagca taacccacaa tactaacctg gatcatagag
      301 gcaagatcat atgcccattt atttgataag gatgttttag aaatttttat aattgtacta
      361 gctgttttcc agcccataac taacaaactc aagtaaatta ttaaaccgat aaaaccatgt
      421 tcaccaatta gttcaaaata aatactatga gcgtcctgat atcttcctcc ctcggctaca
      481 atatcaggag cataggttaa ataattttct ttagtaaaac attcgaatcc tccacccaat
      541 gggcgtgcta atgcaagttt tgtagcaaat tcccaagcta agagccgtcc tttagcagaa
      601 ctatcccttt cttctcctgt actgctaata gttcccattc gctgatgcca tgaatctggc
      661 atgaaattat atagaagtgg tgcaaacaaa ataattacaa taaatggagc aagttttttc
      721 cggcttttta accaaaggaa aaaaccaatt gctacaattg ctaataatgc accgcgtgat
      781 tgagttccta gaatagccac tggtattagt atcatagcga aaactagtcc tgatcggagc
      841 catggctgaa ttgtattgag gtacaaatac caaaataatg ggaatactga aaccaatgcc
      901 aagccaattt ccgtattacc agatataaaa ctacctgctg gccctagtac atgctgacta
      961 ccacctgttg taagcgtaaa tacaccgcct ttgataccat agtaacctat tgataaagct
     1021 ataacccaaa taaaattatg aattttctct ttatcattaa ttatccaagg tatcatcaaa
     1081 gcaattaatt gtattttgat aaccttttcc cactgagtcc aagaaatatc cggttgtata
     1141 gcaaagaaag tggtaatcag aaaccagata tttagaacaa ttaagaggct aaaaattggt
     1201 gaccaaaaat aagtaatatt tttacgagca ataacaagcg ataaaataat ggcagctgcc
     1261 gcaatgattc caaaagggaa attatatgca aacccccacc ctaatcgatg tgggttcata
     1321 taaccaatcc aagcccataa ataaaccccg atctgaggtt tcttaaaaac aaggggcaag
     1381 gctccaaaaa aaataatcgt aactaatata tccctcatga atcaatcatt aatctattga
     1441 agcaatttga tagaaaactt tatctactac agctagtaga gctattggac cttaacccaa
     1501 tatcgatata aaaccgcagg aaatcgaatg agatatgatt gattagattt gccccattgc
     1561 aataataatt tccataattc aaagtaaaat ttgttaatgt aattcgtcat aaactcttga
     1621 ttttggctaa aggaatctgc gaattgcaag gctagatcaa tagcttgttt tttatcctcc
     1681 tgggttgact cccattgata ttctagattc tctggtttag gtaaaggaca aggaacatga
     1741 cctggataag cttgccatgg tatttccaga accttagaat caaattgacc tagccagtcg
     1801 agataaaaaa tatgtcgaag aaatcgatca actggttgct gtactatttc agctataaaa
     1861 ttagcatcaa aaaaaggtaa ttcaaattcg atgcggtcta aatcgatatt ctcaaagtgt
     1921 tcaaccatat ggcgacgctg atcattgaga agtaaaaaca agtagaactg acgacctaga
     1981 tcttctgggt gaatcgaatc taattcagat tgaatgccgt ctgcaagtaa ctgttgagca
     2041 gatgacgcca aagagcgctt cattaattta ggaaatattc cccagcgatt gtaggaagaa
     2101 aaaagttgct ttgcagtggt tagatctttt tttcgagcag catcaattat ttctgaattc
     2161 tggtaaatat gaccaaggcc catactaccg ccatcaccag accagattac tcttggtttt
     2221 atagggttcc atgaaagaaa ttctttggag tttatccaat taatcgtaga aagtttatga
     2281 tatgaatccc caatagttgt tttacctcta ggaagatggc tatgatgtgt acccaacaaa
     2341 tcagctgcca attgaccaaa tacctgatcc tgacttccct gttgagcaaa attggccgtc
     2401 aatacatcta tattaagttc tcttagcgct gcaacgattg cacgtgaatc caggccgcca
     2461 cttaaaaagg ccgcaacgat tttctgatct ttcaatcgaa ttttaatcga ctcgatgaaa
     2521 gtcgcatgaa gctgtttagc aagatgtaaa tcttctgctt tttttgagga taatttgttc
     2581 caatcccaat atttttttct ctcaatttta ttggtatcaa aacacacaat ctcacccgca
     2641 taaagtgaga aaatatcttt atatggtgtt cgatctgata atggataacc aaagcaagcg
     2701 atttcagtaa tgccttttag atcgatttct ttttgatgat gtcttaaaga ctcgagaatc
     2761 cgtagcgcag atgcaaaaat aacaaaatcg gaatgtaccc agaaataaac tggcctcaca
     2821 ccaattttgt cagttacgag aaagagttta ccattctcat ggtcgtagat agccgcgcag
     2881 tatgttcccc tgcagttacg aagtgcttcc aaattatcct tactgatatc agaagcaaga
     2941 agcttcaagc tctctaatcg tgaataaaca ttaccagaat tttgctggag aattggattt
     3001 cctgcgataa atccggatag ctgagaatct gtgaaatggc caagctcacc taatgcacca
     3061 atatcaattt tagcaaaata aatattatta tttttatatt caatcaatgc atttttatca
     3121 tcaggatgac gtgaaattgc tccctgcaac gcttcaatta ttgaatttgg aatactagaa
     3181 ttagagtagc gcgctaaaat ccctgcaaaa atagtcatta aaaatccaat tttttagagt
     3241 gaaatataaa aaattaaccg gatagtaaca ctgatatgta attttattat ttttaaaaag
     3301 aaaccttatt caaggtattt gttcgcatct ggcatcaagg taacttgtct aatatattat
     3361 ttaccagcaa ctaaataata ttccgtcaat ccagctcctg ctcccagcca aaagattaga
     3421 atagctaatt ggactctata ataggttata gcaataaaat atttacgagc caccaacagg
     3481 cagaattcct gaagaacaaa caaagctagc aataggtaac ttatttttac tcgataagag
     3541 atgagtggta ctctgcgagt gcttttctca taagcaaatt gtaccaactg attaattgct
     3601 gagatctcat ccttagggta tgggatatcg ataaaattaa gtatagattc tgaatgattt
     3661 aatttatctt ttgttaccaa gtgaatctgt tcttttgttc gacttagcga aaagttaata
     3721 tcacggtaat tagttatagt ggcttcccat tggtcgtcat gagttagatt aaaaaatgaa
     3781 accgttggaa tcactatatt aggataacgc gtgctgtcat ccaggatgaa aactgctaat
     3841 tgccaatcat cctctgtact tgagtgaact gtgattgtat tagctggcat aggtgtttca
     3901 tcgattacaa cccacccagc aaatggatca atgttgccag ataggatatt cacttcatta
     3961 tttgaacttg ttttgatgac acttatcatt gacttatttt tttgaacatc tgtagctaca
     4021 aaactattaa tttcatctcc tttctgtaat attaagtcag gaaagaaagt ccaatttgta
     4081 cgtgtattac gtggaatggt attattaaat ttatctaata caatccaaat atttttttca
     4141 atttgtatta cttcacgttg taccacaaaa ttttctggac catttcttat catcttgata
     4201 tagaaaattt catcattagc agcaaatttg gttgccctgg cagtacgcat tgatgattta
     4261 ttttcatcga ttaaatgagg tgcattacta ccatcccagg attcggcttt taatcgaagc
     4321 ggatgtccat aaggccaata acccacatta gtgatccaat ttctctcatt cgcccagatt
     4381 aaaatactca attcatctgc aagcttatgt cctaaacctg gaaaatatga ccaagttgta
     4441 acaagttgtg atttagataa aggattgata ctttgatccg ttgggtacca ccaaattgcg
     4501 tacccagctc ctgg
//
LOCUS       DAOQKQ010000194         4435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_11183_length_4435_cov_6.536530, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000194 DAOQKQ010000000
VERSION     DAOQKQ010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4435
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11183_length_4435_cov_6.536530"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            242..532
                     /locus_tag="PKZ49_10970"
     CDS             242..532
                     /locus_tag="PKZ49_10970"
                     /inference="COORDINATES: protein motif:HMM:NF016149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HND37128.1"
                     /translation="MLVKAEPAKRASLTTAPSQVRLWFNEEIEPAFAALSVLDANKKA
                     VTEDKAVVDSEDPKSIILKLPEMQSGRYTVKFRVLSVDGHVVDSEYEFTVKE"
     gene            584..2611
                     /locus_tag="PKZ49_10975"
     CDS             584..2611
                     /locus_tag="PKZ49_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HND37129.1"
                     /translation="MIESIAAISRWAQLVANLVLFGSCIFLLMASSQNSIFKYPWVAK
                     LEMLFPKLAIVVLIGLVGILATTTGQATGIPSDAWQPMVWFEIVKETRMGHLWVARAI
                     SAVIMLLVFIYILRLHRTKWHYLLGAIVAALPLIAGSLVSHSAAEEISFVSIFPYSLH
                     ILLAGAWFGALPAFLLIVFTSRKKEANGRILESSIDGLKQFSKIALPVMLAIVATGFV
                     IANRMVEEDYHALVSSSYGWLLNIKLALLAVILLIAYQARSKWLPLFTSVNNPEETVK
                     GNQLLWKWVRVEFVLAIVLVLFATILANTIPAKHAIIDNWPYPFRLSVDATWSEANVQ
                     PLFWGGAALLLLAVGIAWYAVSNRWEMKRRILIPGILGLSSLAIALPPLAVDAYPETY
                     RNTPVPFDVISISNGAALYAKNCVSCHGPQGKGNGIIAKTFNPPPADLLTEPHTARHT
                     AGDFFHWLTYGIKNTGMPGFSGNLSEEDRWDVVNYMHGMSRGYQGRILNSKVIPEKPS
                     LGPPIFPYFAHDGSSGVIKDFRQKNNVLLVLFSWPQSQERIHELTEAYDHLKKLNTIV
                     LAVPMGNLNKQILAEITKDLPFPVVTEGAQEIVDSYVLYRRTLTHPDILGEGNIPDHL
                     EFLIDRFGYLRARSIPAVDELTWSDMNLLSQQLSQLNQEDEILPPPKDHVH"
     gene            2677..3246
                     /locus_tag="PKZ49_10980"
     CDS             2677..3246
                     /locus_tag="PKZ49_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37130.1"
                     /translation="MVKIMSLIVWGFLMISVSQVAWAHSEEQLKSVETPHGRQLRMAG
                     PFHLELVAKDNELTLYITDHGNKPIGSAGGQGKANIIIASEKDGKKQSVNLEPVFGNM
                     MKAKGDFKVTSDTAIAVLLAIPGYETQGARFTPLATADSVSNHEHDHSHDHDHRDDHS
                     DNHHHDHDKKHDCEHCKAQHKEHHHHHKH"
     gene            complement(3277..3885)
                     /gene="cobO"
                     /locus_tag="PKZ49_10985"
     CDS             complement(3277..3885)
                     /gene="cobO"
                     /locus_tag="PKZ49_10985"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HND37131.1"
                     /translation="MKDLATQAERHRERMQRKKEIVDAAIERANQEKGLLLIHTGNGK
                     GKSSAAFGMIARALGHDMRVGVAQFIKGRSDTGEEAFLRHQPGVEWYVLGEGYTWDTQ
                     NLAQDIETAQRGWQLARTMLQNETINLVVFDELTYPIRYGWLNLDQILIDLENRPAMQ
                     HVIITGRNAPDTLCEIADTVTEFRNIKHAFYSGIKAQKGIDL"
     gene            complement(3927..>4435)
                     /locus_tag="PKZ49_10990"
     CDS             complement(3927..>4435)
                     /locus_tag="PKZ49_10990"
                     /inference="COORDINATES: protein motif:HMM:NF022526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2780 domain-containing protein"
                     /protein_id="HND37132.1"
                     /translation="SSPGQLPSSSGAASVLQQSTEAIQTPTAAINSVPTSDGLIGLLM
                     QQLGVSQTQAEGGTGALLQLAKSKMSPGDFTALGNSIPNMQSIMAAAPSMNTSELGVP
                     KSLAGMTGGNMPGANSGMLGIAGAFQQLGLAPDMVQKFIPVITQFVQGNGGGAVTSAL
                     QSALMGGL"
BASE COUNT         1269 a          858 c          981 g         1327 t
ORIGIN      
        1 cttgcaatcg ctgccggtat cgccttcttc ttccttaaat ctaataaaaa agcgtgagga
       61 aaaaatcgaa cgctcttaaa taaaactact ctaattattg tttattagta aaactaaaat
      121 aaggagcaac aacgtgaagg ggatagcagt aggccgatca aatagaaatc tcagttgggt
      181 agtgttactg atagtcagta tagtatgttt cttcaatata agttctgtat ttgcgcatgc
      241 catgctcgtt aaagctgagc ctgcaaaaag agcttcactt acgacagccc cttcacaagt
      301 gcgcctttgg tttaatgaag agatcgagcc tgcatttgca gcattatctg tacttgatgc
      361 gaataaaaag gcagtaaccg aggataaagc tgtagtcgac tcagaggatc cgaagtctat
      421 cattcttaaa ttgcctgaaa tgcaatcagg tcgttatacc gtcaaattta gagtattgtc
      481 agttgatggt catgtggttg actcagaata tgagtttaca gtaaaggaat aactgagaaa
      541 gaatagcgcc gcatcaaaaa aagaaagtta aaaaataaca ataatgattg agtcaattgc
      601 agcaatttca cgttgggcac aactagtagc caatctggtt ctattcggta gttgcatttt
      661 cctgctgatg gccagtagtc agaattcgat attcaagtat ccatgggtgg ccaaattgga
      721 aatgcttttt ccaaaattag ctatcgttgt attaattgga ttagtaggga tacttgcaac
      781 aaccacgggg caggcgactg gaattccatc tgatgcatgg cagcctatgg tttggtttga
      841 aattgttaaa gaaacccgta tggggcattt atgggtagct agggcgattt cagcagtgat
      901 aatgctatta gtttttattt atattttgag attgcataga actaaatggc attatctatt
      961 aggtgcaatt gtagcagcat tacctttgat agcaggttcc cttgttagcc attcggcagc
     1021 tgaagaaatt tcgtttgttt ccattttccc ttactcattg cacattctgc tagcgggtgc
     1081 atggtttggc gcattacctg cttttttatt aattgttttt acttctcgta agaaagaagc
     1141 gaacggtaga atacttgaat ccagtataga tgggttaaag caattttcta agattgcact
     1201 gccagtcatg ttagcaatcg tggctaccgg ttttgttatt gcaaaccgta tggtggaaga
     1261 agattatcac gcccttgtgt cgagttctta tggatggcta ctaaacatta agttagcgct
     1321 attggcggtt attcttttga ttgcctatca agcgcgctca aaatggcttc cgttatttac
     1381 ttcagtaaat aatccagaag aaacggtaaa aggtaatcaa ttattatgga agtgggttcg
     1441 agttgaattc gtactggcta ttgtgttggt attgtttgct acgattctag cgaatacgat
     1501 tcctgcaaag catgccatta ttgataattg gccttatcca tttcgtttgt ctgtggatgc
     1561 aacatggagt gaagccaatg tacagccttt attctggggt ggagcagctt tacttttgtt
     1621 agcggtcgga atagcttggt atgccgttag taatcggtgg gaaatgaagc gtagaatact
     1681 aataccgggg attctagggt tatcatcact tgccatcgcg ctcccaccgc ttgcggttga
     1741 tgcttatccg gaaacttatc gaaatacgcc cgttcccttc gatgtgattt ctatttctaa
     1801 tggcgctgcg ttatatgcca aaaactgcgt gagctgtcat ggtccacaag gaaaaggcaa
     1861 tggaattatc gctaaaacat ttaaccctcc tccagcagat ttattgacag aaccccatac
     1921 tgcaaggcat acggcgggtg atttttttca ttggttgacc tatgggatta aaaatacagg
     1981 gatgccaggc ttttcgggaa atttatcgga ggaagatcgt tgggatgtgg tgaattatat
     2041 gcatggaatg tcgcgtggat atcagggaag aatacttaat tccaaagtta tcccggagaa
     2101 accgtcatta ggtccaccga tatttcctta ctttgcccat gatggaagta gtggcgtcat
     2161 caaggatttt cgccagaaaa ataatgtgct gttggtgtta ttttcttggc cacaatccca
     2221 agaaagaatt catgaactga cagaagctta tgaccatttg aaaaaattga atacgatagt
     2281 acttgcggtt ccaatgggta atctgaataa gcagatacta gcggaaatta caaaagatct
     2341 accttttcct gtagtgacgg aaggtgcaca agaaattgtg gatagttatg ttttatatcg
     2401 aagaacattg acccatccag atattctggg tgaagggaat attcctgatc atttagaatt
     2461 tttgattgac cggttcggtt atttgcgcgc tcgctcgatt ccagcagttg atgaattaac
     2521 atggtcagat atgaacttat tgtcacagca attgagtcaa ctgaatcagg aagatgagat
     2581 tcttccacca cccaaagacc atgtgcatta gctcttatta tgaattgcta tgcaataatg
     2641 ttgttttcta atgattaaaa tcaagaggat aagaaaatgg taaaaattat gtctctcatc
     2701 gtttggggtt tcttaatgat cagtgtttct caagtagctt gggctcatag tgaggagcag
     2761 ttaaagtcag ttgaaacgcc gcatggtagg cagcttcgta tggcaggacc gtttcatctt
     2821 gagctggttg caaaagataa tgaattaacg ctatatatca ccgatcatgg taataaaccg
     2881 attggatcgg caggtggcca aggtaaagca aatattatca tcgcgagtga gaaggatggt
     2941 aagaaacaat ctgttaatct ggaaccggta tttggaaata tgatgaaagc gaaaggtgac
     3001 ttcaaggtaa cgtctgatac cgctattgct gttttattag cgattccagg ctacgagaca
     3061 caaggtgcac gatttacacc attggcaaca gcagattcgg tgagtaatca tgaacatgac
     3121 cactctcatg accatgatca tcgtgatgat cattcggaca atcatcatca cgatcatgat
     3181 aaaaaacatg attgtgaaca ctgtaaagcg caacataaag agcatcacca tcatcacaaa
     3241 cattaatcat cgatttgcag ttaaagatga ttaatttcag agatcgatcc ctttctgggc
     3301 tttgatacct gaataaaagg cgtgtttaat attacgaaat tcggtaacgg tatcagcaat
     3361 ttcacatagc gtatctggtg cgttacgacc tgtaatgatg acatgttgca ttgcaggtcg
     3421 attttccaaa tcaattaata tctgatcaag attgagccag ccgtaacgaa ttgggtaagt
     3481 gagttcatcg aaaacaacga gattgatggt ttcattctgc aacatagtac gagctaactg
     3541 ccatccgcgc tgcgctgttt ctatatcttg agctaggttt tgggtatccc aggtgtagcc
     3601 ttcgccgagc acataccatt caacacctgg ttgatgacgg agaaaagctt cttcaccggt
     3661 atctgatcgt cctttaataa actgagcaac accgacccgc atatcatgtc ctaatgcacg
     3721 ggctatcatc ccaaaggccg cacttgattt tccttttccg tttccagtat ggatgagtag
     3781 caagcctttt tcttgatttg cacgctcgat tgctgcgtca acgatctctt tttttcgttg
     3841 catgcgttct cgatgacgct cagcttgggt ggctagatct ttcatcaatt aatgaacaaa
     3901 cagattatct ggtggataaa cttctactat agtccaccca tcaaagcgga ctgtaatgcg
     3961 ctggtgaccg cacctcctcc atttccttgg acaaattgag taatgactgg aataaatttc
     4021 tgcaccatat cgggggctaa gcctaattgc tgaaatgcac cagctattcc taacataccg
     4081 ctattagcgc ctggcatgtt acctcctgtc atacccgcca aacttttagg aacaccgagt
     4141 tcgcttgtat tcatcgaagg cgctgctgcc atgatacttt gcatattggg tatcgaatta
     4201 cctaacgctg taaaatcacc tggcgacatt tttgatttag ctaactggag taatgcacct
     4261 gtaccgcctt ctgcttgagt ttgagaaaca ccaagctgct gcataagaag tccgatcaaa
     4321 ccatcgcttg taggaacaga atttatcgcc gcagtagggg tctgaatggc ttcagtgctt
     4381 tgttgtagaa cacttgctgc cccagaagaa ctaggtaatt gcccgggact tgagc
//
LOCUS       DAOQKQ010000195         4403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_11323_length_4403_cov_4.913753, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000195 DAOQKQ010000000
VERSION     DAOQKQ010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11323_length_4403_cov_4.913753"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PKZ49_10995"
     rRNA            <1..246
                     /locus_tag="PKZ49_10995"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            306..418
                     /gene="rrf"
                     /locus_tag="PKZ49_11000"
     rRNA            306..418
                     /gene="rrf"
                     /locus_tag="PKZ49_11000"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(741..878)
                     /locus_tag="PKZ49_11005"
     CDS             complement(741..878)
                     /locus_tag="PKZ49_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37133.1"
                     /translation="MYQVVANNKELLAALETKIASEGVILNDAQIVALKKRNRMISSP
                     R"
     gene            complement(1228..2175)
                     /locus_tag="PKZ49_11010"
     CDS             complement(1228..2175)
                     /locus_tag="PKZ49_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37134.1"
                     /translation="MNTNLIDSSQEVLSDDSSNKQYTFTIENGVVKAYFEVENGVSEQ
                     ESIDNRDTFVINGNQITHTKQTSNGLETEVFVDSDGDGFYTRTSQSNQNGNDDDSNDD
                     NGNDDNGNDDDSSGNHKVFKFEINNGQVTAVFELKNGVLEPKSLDDNGTKSYTVSGND
                     VIRTEIKPFGTEITRYSDADGDGLFVRVSEQWQVSTNPSGVVPRFTETITFTPTSNDD
                     NIAVRGGEHCRGGQGADDFIFREADHLRIEDFSSQTGDALIFDTGLGLTSKEHLASFI
                     TDIHHDGQNFIVNFGSTVSITLIGVQPDQISWDDVSVLS"
     gene            complement(2469..3449)
                     /locus_tag="PKZ49_11015"
     CDS             complement(2469..3449)
                     /locus_tag="PKZ49_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HND37135.1"
                     /translation="MHFNQQPLLLFIGLFFFAITSLLVGISFGSVDISWSEIAQILSN
                     PSEEPHAIIIWELRLPRVLTAFACGSLLALAGALLQVLLRNPLADPYILGISGGASVG
                     ALTAMMLGWSTTSINIASSAGALTIIGLIFSLNFRSPTWNIYRLLLIGVVLSAACNAV
                     ITLLLALAPAHDTKGMLFWLMGDLSRSQASLSTWGVLIILCIISTLLASEFNVLSIGQ
                     LKARSLGIAVNILQILIFLIASIATVTALMQAGAIGFIGLLMPHAIRLVGVNDYRWLI
                     PCVILLGGGFLVFADTLARTLWAPQQLPVGVLTALLGAPLLLILLIRKRF"
     gene            complement(3506..3796)
                     /gene="rnpB"
                     /locus_tag="PKZ49_11020"
     ncRNA           complement(3506..3796)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKZ49_11020"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(3847..>4403)
                     /locus_tag="PKZ49_11025"
     CDS             complement(3847..>4403)
                     /locus_tag="PKZ49_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HND37136.1"
                     /translation="IAFDLAEQEISKAKAGHYPTLDLVAQYTDQQGVGGAITGRGIDL
                     TSKSVGLQLNVPIFQGFSVQSRVREALANRDRARMDLENIRRTIGLQVRQQYLNVTNG
                     IAQVKALKQALTSSKSQLDSTLLGQEVGVRTEVDVLNAQQQLFSARRDLVQAYYNYLM
                     ARLRLKAEVGELDEEVLQEINALL"
BASE COUNT         1300 a          911 c          854 g         1338 t
ORIGIN      
        1 cgagaggacc ggagtggacg aacctctggt gtaccggtta tgacgccagt cgtacagccg
       61 ggtagctaag tatggaaaag ataaccgctg aaagcatcta agcgggaaac ttgccatgag
      121 ataagatttc cctgggaaaa tattccccta aaggctcgtt gtagatcaca acgttgatag
      181 gtcgagtgtg taagcgcagt aatgtgttta gctaatcgat actaattggc cgtgaggctt
      241 gatcctataa atatattatt actaaattac ttataaatat tgaatattta tatattaaag
      301 ttttgcttgg tgaatatagc gctttggaac cacttcttcc cataccgaac agaatcgtga
      361 aacgaagttg cgccgatgat agtgtgtata cgcatgtgaa agtaggacat cgccaagctt
      421 ttcatattta gcaaaattga ttgaatgatt gtgaagtgag attagattta attgagattt
      481 tttctgcttc aaatcatttt cccactgagc taaatattca taggattatt tcatgataaa
      541 tccttccatc attataataa tcgattcagc tgcctaagtc tttttgcaat gactccaaaa
      601 aatggcagca tcttattatt taagatatca gctgcagtaa taagcgtttt cgccgtatag
      661 aaattgcaat acgctcttca gatttatcac ataaaaaagt accctgtgca ccaaaataaa
      721 caggatacgc tgtttctatt tcaacgtggc gatgatatca ttctgtttct ttttttcaat
      781 gctacgattt gtgcgtcatt caaaatgacg ccttcactgg cgatcttggt ttctagtgca
      841 gcaaggagtt ctttgttgtt tgctactacc tgatacataa agatgttggc tatgtgttgg
      901 atattctacc gcataggtta ccaccgccct attagcaata cgacgcggat cctgttgatc
      961 aatgcttcca gaccattatc tttcaggttt ttgagacgtt ctggagttct tcggcaagat
     1021 tgagcaatcc tgttttatga ttggattgtt agtttgattt taaatttcaa catctatatc
     1081 aaaccttagc tttattttcc tgcaagcaaa ttttcatttt ctgatagata attaatgcta
     1141 acgcattgaa ttatttattt taaaataact taacttactt ctagactaac agatctagtc
     1201 tgctagccta gagccccata cttttaatta gctaagaacg gaaacatcgt cccagcttat
     1261 ttgatctggt tgcactccaa tcaatgtaat ggatacagtt gacccaaaat ttactataaa
     1321 attttgtcca tcatgatgaa tatcggtgat aaagctagct aagtgttctt ttgatgttaa
     1381 gccgagtcca gtatcaaaga ttaaagcatc accagtttgt gaactaaaat cttcgatacg
     1441 aaggtgatcg gcttcgcgaa aaatgaaatc atctgcacct tgcccaccgc gacaatgttc
     1501 tccaccacga acagcgatat tgtcatcatt gctggttggg gtaaatgtaa tcgtttcagt
     1561 aaatctggga acaacgccag atgggtttgt agagacttgc cactgctcgg atacgcgaac
     1621 aaaaaggccg tcaccgtccg catcggaata acgagtaatt tcagtgccaa atggtttaat
     1681 ttcagtacga attacatcgt tgccactcac cgtatagctt ttcgttccat tatcatctaa
     1741 ggatttaggc tcaagtacgc cattcttcaa ttcaaatact gctgttactt gaccattgtt
     1801 gatctcaaac ttgaaaactt tgtgatttcc agaggaatcg tcatcatttc cgttatcgtc
     1861 gttcccgtta tcatcattac tgtcatcatc attgccattt tgattcgatt gagaagtacg
     1921 ggtatagaaa ccatcgccat cggaatcgac aaagacttct gtttcaagtc cattggaagt
     1981 ttgtttggta tgtgtgattt gattgccatt aatcacgaat gtatctctgt tatcaattga
     2041 ttcttgttcg gaaacaccat tctcaacttc gaagtaggct tttactacgc cattctcaat
     2101 cgtaaatgtg tattgtttat tactactatc gtccgataaa acttcttggc tcgaatcgat
     2161 taaattagta ttcattttgt ttctcctagt tattagtaag aaagtggata gagaaagcaa
     2221 aagaattttt attattactt ttttctatcc tttacataaa tatagaaagc gtttgcatct
     2281 cgttgcggat ttgctaaacc caatgaatta cggattagtg ggttgagaaa tcatgtctac
     2341 cgagatagtc agactctcca tcattcttat ggtcgaatcc agtgcatttc atcaatttct
     2401 attttatgtg tgaaaaattc ccatgtcaaa tatattttta gccctttttt tatctaccca
     2461 atcataattt agaagcgttt tcttattaat agaatgagca gcagcggagc accgagtagg
     2521 gcggttaata ctccaacagg tagttgttga ggggcccata atgtacgtgc cagtgtgtct
     2581 gcaaatacta gaaatccacc gcccagtaat attacacagg gaatgagcca gcgataatca
     2641 tttacgccaa ctaaccgaat tgcatgaggc attagtaacc caataaaacc aatagcaccg
     2701 gcttgcatca atgctgttac ggtagcaata gatgcgatca gaaaaattaa tatttgtaaa
     2761 atattgacgg caatgccgag agaacgtgct ttaagttggc cgatacttag tacattgaat
     2821 tcacttgcaa gtagtgtact gataatgcac agaataatta agacacccca agtagatagt
     2881 gatgcttgag aacgggaaag atcacccatt aaccaaaata acatcccttt cgtatcatgt
     2941 gcaggagcaa gtgctaataa aagagtaatg actgcattac atgccgcaga tagcactacc
     3001 ccaattaaca ataagcgata aatattccaa gtcggtgaac gaaaattcaa actaaaaatt
     3061 aatcctataa tcgttaacgc acctgctgat gacgcaatat tgatactagt cgttgaccat
     3121 cccagcatca ttgcagttaa agcaccgact gaagcacctc cagaaatacc cagaatataa
     3181 ggatcagcaa ggggattgcg caataagact tgtaataaag ctcctgcaag agcaagtaag
     3241 cttccacacg cgaaagcggt tagtactcgg ggtaatcgca attcccaaat aataatagca
     3301 tgcggttctt cactaggatt gctcagaatt tgagcaatct ctgaccagct tatatcaaca
     3361 ctgccaaatg atatgccaac caaaaggcta gtgatcgcaa aaaagaataa accaatgaaa
     3421 agcagcaaag gttgttgatt gaaatgcact gattcgtgat ctaccgtata gtatagcgta
     3481 gatttataaa gaaattattc gtagggggag aagctgaccg ataagccggg ttctgtcatg
     3541 gacagtcatt cctctaggca tacggttacc catacactca agcaacctac ccgcagattc
     3601 ggcgagcaac gtcaacacct gcctatttgg tcttgctccg gatggaggtt accgcgtttc
     3661 accgtaactc aatacgctcg tctctgtggc cctattcctc acttcacagt ggtcggccgt
     3721 tagccgacat cccgctcttt ggagcccgga ctttcctctc cctgcaaata caggcagcga
     3781 ctgtctagtc agcttcagtt tgtattctat caattctttc tattcgatgg gtagaaaacc
     3841 atttccttag agcaacgcat taatttcttg caaaacttct tcatctaatt caccgacttc
     3901 tgctttgagc cgtagtcgtg ccatgagata attatagtag gcttgaacaa gatcgcgtcg
     3961 cgctgagaat aattgctgct gggcatttaa tacatcgacc tcggttctaa cacccacttc
     4021 ttggccaagt aaagtagagt ctaactgact tttgctagag gtcagcgcct gctttaaagc
     4081 tttgacttga gcgattccgt tagtgacatt caggtattgt tgacgtacct gtaacccaat
     4141 tgtgcggcga atattctcta aatccattcg tgctcgatca cggttagcca gggcttcacg
     4201 aactcgtgat tgaaccgaga atccttgaaa aattggcaca ttcagttgca ggccaactga
     4261 tttagaagtg agatcaatgc ctcgacccgt aatagcgcca ccaacgcctt gttggtcagt
     4321 gtattgtgca actagatcca aagtgggata gtgccctgct tttgctttac taatttcttg
     4381 ctcagctaaa tcaaaggcaa ttt
//
LOCUS       DAOQKQ010000196         4402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_11327_length_4402_cov_4.656085, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000196 DAOQKQ010000000
VERSION     DAOQKQ010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11327_length_4402_cov_4.656085"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(296..1168)
                     /locus_tag="PKZ49_11030"
     CDS             complement(296..1168)
                     /locus_tag="PKZ49_11030"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HND37137.1"
                     /translation="MIENSDALNLLFATDLSSRCDRALDRAVQLAQSWEAKLLVVHAV
                     DPAYAVRYAKIIQDDLPSWRRPEDYRTVAARRLSMDLAIDKIDAEVYVAEGIPHEIVI
                     EAVRRKNSNLVITGLGRDEVLARTQFGSTLDKLLQELPIPLMIVRRRVRGPYRHVVVA
                     TDFSPASQPALETAMRWFSNTQLTLFHAYEGVGLGGGTLANEAWSKIANDQCDSFLAR
                     MALDPIDVQRIDRVIEKGHPEVLLNDYVSHKEIDLVVLGTNGRSGFLKSMLGSTAENL
                     LHLLECDTMVVRSQ"
     gene            1428..2363
                     /locus_tag="PKZ49_11035"
     CDS             1428..2363
                     /locus_tag="PKZ49_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HND37138.1"
                     /translation="MCLVSLYLAVSSDTQADEGGTSFWLPGQFGSHAATLTKPGWSLT
                     LSYHHASTRTATGKNFEIGGRIETGVRNQTHLLFITPTYTFSEFVLGGQAAISLTGTF
                     GHMNVSADATLAGPNGNVLSRNDRSSLTGIGDLFPFATIRWNAGNHNFMTYTMLGVPV
                     GDYKIGQLTNLGINHWSIDIGSGYTYFNTQAGREFSVVAGFTHNFKNHDTQYRNGNNL
                     HLDWSISQHLSKNWHAGLVGYYYYQITGDSGSGALLGDFKSRINGIGPQVSYQFMLGK
                     REYHLNLRGYKEFGAENRPEGWNAWITITIPLEGR"
     gene            complement(2631..>4402)
                     /locus_tag="PKZ49_11040"
     CDS             complement(2631..>4402)
                     /locus_tag="PKZ49_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HND37139.1"
                     /translation="IRRLPAVEALGSVTVICTDKTGTLTRNEMTVQRIITTHHQLEVS
                     GVGYQPQGGFSENNQAVCLDTYQGEHRELYDLIRTGLLCNDAQLRQQGDDWCVEGDPT
                     EGTLITLALKADLKPILEHESLPRIDIIPFESQHRFMATLHHKYPDTGMVLIKGAPEK
                     ILSMCVNEGSWNHHCPLNIGYWHQKVQASAMQGQRVLALAAKSVDIQQTTLNAEQVLG
                     GFTILGLVGIIDPPREEAIRAVSLCQSAGIRVKMITGDHATTAQAIGMQLGIGDGKTS
                     LSGQEIEQMDAVALQSAVLENDVFARASPEHKLRLVEALQARGEIVAMTGDGVNDAPA
                     LKRADIGVAMGKKGTEVAKEAAKIIITDDNFASISQAVQEGRTAYNNIQKTILYILPT
                     NGGEVGIVIIAILLGITLPITPLQILWINMVTETTLSLSLAFEKTEPNIMQRSPRKPS
                     DPIMSGLMFWRVILVSFLLTISGLALFFWELTQNASIEEVRTIVVNALVMGEICYLLN
                     CRYLYSSSLSYQGIFGNRYALSAIGLLAILQLMFTYHPLMQQWFGTAAIDLVAWFKIL
                     TLGVGLFLIIEFEKWLMRRYRAN"
BASE COUNT         1230 a         1051 c          856 g         1265 t
ORIGIN      
        1 ccatcgcgct gtagccttac cgctgcttca gcttgagaaa gcccatttgc cgatgcctca
       61 aggccagtca tgatttcttc aggcaattga ctgtgccata gaatgtttga gcggttctga
      121 gtatcgatca tctcacccct cttatttaat gcgtgaattt atgatagttt tagattcata
      181 ctaaaactga tgaattccat gttacgcctc actaccctag atcgagtcat aatctacctt
      241 aacgtgattt actcgcttca gaaagaaagg aactcaccat cacctcaata gaatgttact
      301 gactgcgcac aaccatagta tcgcattcta ggagatgcaa tagattctcg gctgtacttc
      361 caagcatact tttaagaaat ccactgcgtc cattcgtacc aagaactact aggtctatct
      421 ccttgtgact tacataatcg ttcaacagta cttccgggtg acctttctca attacacgat
      481 caatgcgttg gacatctatt ggatcaagtg ccattctggc aagaaagctg tcacactgat
      541 cattggcaat tttactccat gcttcgtttg ccagtgtacc tcctccaagt ccgacgccct
      601 cataagcatg gaacaatgtt agttgtgtat tactaaacca gcgcattgca gtctctagcg
      661 ctggctgcga tgctggtgaa aaatctgttg ctactaccac atgccgataa ggacctctta
      721 ctcgacggcg tacgatcatt aatggaatcg gtagttcttg caacaattta tcgagggtgc
      781 tcccaaattg agtacgagcg agaacttcat cgcgccccag gccagtgata actagattgc
      841 tattttttcg acgtacagct tcgattacga tttcgtgggg aatcccttcg gccacatata
      901 cttcagcatc tatcttgtcg attgccaaat ccatacttaa tcgacgagct gcaactgttc
      961 gataatcttc gggacgtcgc cacgaaggaa gatcatcttg tattatcttc gcgtagcgaa
     1021 cagcataggc aggatctaca gcatgtacca cgagtaattt agcttcccaa gattgggcta
     1081 gctgcaccgc acgatctaat gctcgatcac atcgactgct aagatcagtg gcaaataata
     1141 aattaagagc atcagaattt tctatcatat aaaattcccc ttcaaaaaaa tagtcagcct
     1201 acatcgctaa tcaataattc ttaattcttc tcaaatttgg aaggattcga cagttggaat
     1261 ttgaaaaaga tcaacaatcg attctcactt tttttagtac ccaatctgac ttaccatagt
     1321 tgaccagctg ggataagttt atattatacg caccttcacc gatgggtcgt ttttcgattt
     1381 tctttaatac cttaagaaaa aaccacaggt ctgcatcgct tagttttatg tgcttggtat
     1441 cgttgtattt agctgtctct tcagatacgc aagctgatga aggcggcacg agtttttggc
     1501 tacccggaca atttggcagt cacgccgcca ccctaacaaa acctggttgg tctcttacgt
     1561 tatcttacca ccacgcttct acgagaacag ccacaggcaa aaatttcgaa attggcggca
     1621 gaattgaaac aggtgtgcga aatcaaactc atttgttatt tattacacca acgtacacct
     1681 tttcggaatt cgtacttggt ggtcaggctg caatcagttt aaccgggaca tttgggcata
     1741 tgaatgtttc tgctgatgct actctagctg gccctaatgg gaatgtattg tcacgcaatg
     1801 atcgcagctc acttactggt atcggagatc tctttccttt tgctaccatt cgctggaatg
     1861 ctggcaatca caactttatg acttacacaa tgttaggagt tccagtaggc gattataaga
     1921 tagggcagct gacaaacctt ggtatcaacc actggtcaat agacattggc agcggctata
     1981 cttacttcaa tactcaggcc ggacgtgagt tctcagttgt tgcaggtttc actcacaatt
     2041 ttaaaaacca cgatactcaa taccgcaatg gcaataactt gcatcttgat tggagcattt
     2101 cacaacactt gtcaaaaaac tggcatgccg gattagtcgg ttactattat tatcaaatta
     2161 ctggagatag cggatcgggt gcactcttag gcgacttcaa atcacgcata aatggaattg
     2221 gcccgcaagt gagttatcag ttcatgctag gcaagcggga ataccatcta aatctcagag
     2281 gttataagga atttggtgca gagaataggc cagaaggctg gaatgcttgg attacgataa
     2341 cgatcccact tgagggtcgg tagtagtcga aaattacgac caaacgaata atctggcact
     2401 gaagcgtaag tttttcaggg taatagcatc aaaaaactac tggcccagat tttccattaa
     2461 aatattttct aatggaaatt ggaaatcaca tttaacctat atttgagctt gtgataaata
     2521 tcatctgatc aagctcaatc gtattgacct tcctttaagc aatcattttc catgtcagca
     2581 cgcaagaaca gattttcaga ctaaaaacac atgaattttt cgacagtctg ttaatttgct
     2641 cggtaccgcc tcatcaacca tttctcaaac tcaatgatca agaaaagacc aacgcccagc
     2701 gttagtattt taaaccatgc cacaagatcg atagctgccg ttccaaacca ttgctgcatc
     2761 agaggatggt aagtaaacat caattggaga atcgccaata ggccaattgc cgatagcgca
     2821 taacggttgc caaaaatacc ttggtaagat aaggaagagg aatataaata gcgacagttg
     2881 agtaagtaac aaatttcacc catgaccaat gcgttaacta cgatcgttct gacttcttca
     2941 atactagcgt tttgtgtcaa ctcccagaaa aacaaagcta acccactgat agtcaataaa
     3001 aatgaaacca gaataactcg ccaaaacatt aatccagaca ttatcggatc cgatggtttg
     3061 cgtggggaac gttgcataat attgggttct gttttctcaa atgcgagcga taaagaaaga
     3121 gtggtctcgg tcaccatatt gatccataaa atttgtaatg gtgtaatggg aagggttatt
     3181 cccagtagta ttgcgataat gactatcccg acctctcccc catttgtcgg caagatatat
     3241 aaaatcgttt tctgtatgtt gttatacgca gttcgacctt cctggactgc ctgagatatc
     3301 gaagcaaagt tatcatcagt aattataatt ttggctgctt ccttggccac ttccgttcct
     3361 ttctttccca tggccacacc aatatccgct cgtttcaatg ccggtgcatc attgacacca
     3421 tcacctgtca tggccacgat ttcgcctctc gcttgtaacg cctcaaccaa tcgtagctta
     3481 tgctctggac tcgcacgggc aaacacatcg ttttctaata ccgcagattg cagcgctacc
     3541 gcatccatct gttcgatttc ttgaccactt aacgatgttt tcccatcacc aatgcctaac
     3601 tgcatcccaa tcgcttgggc cgtcgttgca tgatcacctg tgatcatttt cacacggatt
     3661 ccagccgatt ggcacaaact tacagctctg atggcttctt cacgcggtgg gtcgatgatt
     3721 cccactagcc ccagaatcgt aaatccacct aacacttgtt cagcgttcaa cgtagtttgc
     3781 tggatatcga cgctcttagc cgcaagcgcc aagacacgct gcccttgcat tgctgatgct
     3841 tgcacttttt ggtgccaata gcctatattc aaagggcaat gatgattcca gcttccttca
     3901 ttcacacaca ttgataaaat cttttcaggt gcacctttta tcaacaccat accagtatca
     3961 ggatatttat gatgcagtgt tgccataaaa cgatgctgcg attcaaacgg tatgatatcg
     4021 attcgcggta atgattcatg ctccagtatc ggtttaagat ccgcttttag cgccaacgtg
     4081 attaatgtac cttccgttgg atcaccttcc acacaccaat catcgccctg ttgccgcagc
     4141 tgcgcatcgt tacatagcaa tcccgtccga attaaatcat agagttctcg atgttcacct
     4201 tgataggtat cgagacaaac cgcttgatta ttctcactaa aaccaccctg cggttgatat
     4261 cctaccccac taacttctaa ttgatggtgt gtggtaataa tgcgttgcac cgtcatctcg
     4321 ttacgcgtta acgtacccgt tttatcggta caaatcacgg taaccgaccc taacgcttcg
     4381 actgctggca gacggcgaat aa
//
LOCUS       DAOQKQ010000197         4345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_11535_length_4345_cov_6.014918, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000197 DAOQKQ010000000
VERSION     DAOQKQ010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11535_length_4345_cov_6.014918"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1609
                     /gene="yidC"
                     /locus_tag="PKZ49_11045"
     CDS             <1..1609
                     /gene="yidC"
                     /locus_tag="PKZ49_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HND37140.1"
                     /translation="DNNSVPQQGPDSPPIPGDALVSSQSNQDRASGIDAASASTGTPR
                     NLPTGEQIKVITDMVIAEIDTAGGDVRKLELLKHPSRENKEIPYALLHSDATQTSVAQ
                     SGLIGEGFPTHKTQYTLETKVREYSLEADQDKIEVRLVAPRENEVQVTKIYTFNRGSY
                     VIDVRFEIDNQSEEIVRPFAYFQMLRDSNPPAGATMMIPTYLGAAIYTEDEKFQKIQF
                     SDLDKNKADYPRNADNGWIAMLEHYFLSAWLPPQKAPREYFAKRQNDNLYTAGVISPV
                     GAIDPGKTTSISMPLYAGPQEQEKLAELAPGLELTVDYSWLTVIAAPLFWLLSFYHEW
                     VGNWGVAIILLTMTVKLLFFPLSAAGYRSMAKLRLVTPKLQRIQEQYKSDRQQMHQAM
                     MELYKEEKINPLGGCLPILVQIPVFIALFWVILAAVELRYAPFALWITDLSSPDPLYV
                     MPLLMGISMFIQFKLNPKPTDPIQAKVLQIMPIAFSIFFFFFPAGLVLYSLVNNILSI
                     LQQWQITRLYGQPSSNDASTKKKVKEK"
     gene            1733..3088
                     /gene="mnmE"
                     /locus_tag="PKZ49_11050"
     CDS             1733..3088
                     /gene="mnmE"
                     /locus_tag="PKZ49_11050"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HND37141.1"
                     /translation="MYSSDIIAAIATPPGRGGIGIIRISGNNLKEITQQLLGDLPKPR
                     YASLNYFLDQNHEVIDHGIAIYYVAPHSYTGEDVLELHGHGGPAVINLLLKRCLALGA
                     RLAEPGEFTLRAYLNNKIDLVQAESIADLIDASTAGAARSALRSLQGKFSEAIHELVR
                     ALIDLRIQVEAALDFPEEDIDWLETTHLKTQLNQLYEKLEKVYVSSQRGNLLREGAKI
                     VLIGQPNVGKSSLLNRLAGDEVAIVTEIPGTTRDAIQQLIQIDGVPLHIIDTAGLRET
                     EDIVEKKGIERTYTAMEQADLALLLIDSQNELSEADQNLLSKLPVNLPVLIVFNKIDL
                     LNQPAKSEDKNGYSMIYLSAKTGVGIEILCEKILTLVGWRSHVAGEGIFMARQRHLQA
                     LDQAQRHLSNAIAWASSGKAIEVLAEELRLAQYSLSSIVGEFSADDLLGEIFSHFCIG
                     K"
     gene            3287..>4345
                     /locus_tag="PKZ49_11055"
     CDS             3287..>4345
                     /locus_tag="PKZ49_11055"
                     /inference="COORDINATES: protein motif:HMM:NF040606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme-cytochrome C peroxidase"
                     /protein_id="HND37142.1"
                     /translation="MKRYLKPAVLVIFSVFFGFNLLHTHAKDDFSEIVLVDGKVQSRV
                     ELDQGWSQEIRQLFWFTSQGSRIIPYNWFAWLEQADNNSLFRNVEHMEMLRYLPMNSS
                     KLNPAGLPIGFALDTDHSTGRKWVGLTCAACHTNQIDYQGTRMLIEGAPTLANFVKFF
                     DDLVNALNKTNDNPQKFTRFAKNVLGNQYSETTANKLHQELDQIAAKLAQRRMVNQLP
                     PDYPEDFTSYARLDAFGNIQNAASAFALHDLTNRNAPTAPVSYPFLWGTHQSDVVQWN
                     GSAPNTPIIGPLIRNMGEVVGVFGDLEISEAPIWKKLLGIKVEYNAHVDIHNLGKLEL
                     WVKHLRAPSWSDGNIELAV"
BASE COUNT         1276 a          855 c          954 g         1260 t
ORIGIN      
        1 tgacaataat tccgttcccc aacaaggccc tgattcacct ccaatacccg gtgacgcgct
       61 cgtctcttct cagtcaaatc aggatcgcgc atcagggatt gatgccgctt cagcatccac
      121 tgggacacca cgtaacttac caactgggga gcaaatcaaa gttattactg atatggtaat
      181 cgctgagatt gatacggctg gtggggatgt gcgtaaatta gaattactta aacatccctc
      241 aagggaaaat aaagaaatac cttatgcgct tttgcatagt gatgctacgc agaccagcgt
      301 agcgcaatct gggctaatcg gtgaaggatt tcctacccac aaaacgcaat ataccttaga
      361 gactaaagta cgtgaatata gtctcgaagc tgatcaggat aaaattgaag ttcggctggt
      421 tgctccacgg gaaaatgagg ttcaagtcac taagatttat acattcaatc gtggaagcta
      481 tgtcattgat gttcgatttg aaatagataa tcaaagcgag gaaattgttc gcccatttgc
      541 gtattttcaa atgttgcggg acagtaatcc gccggctggt gcaacgatga tgattccaac
      601 ttatctcggt gctgcgattt atactgaaga tgagaaattt cagaaaatcc agttttcaga
      661 cttagataag aataaggcag actatcctcg gaatgcggat aatggctgga ttgcaatgct
      721 ggagcactat tttctttctg catggcttcc tccacaaaaa gcgcctcgcg aatattttgc
      781 aaagcgtcaa aatgataatt tatatactgc aggagttatt tccccagtag gtgcgattga
      841 tccaggaaaa acaacgagta tttcgatgcc gctttatgca gggccgcagg agcaagagaa
      901 actagcagaa ttggcgcctg gcctcgaatt gactgttgac tacagctggt taactgtgat
      961 cgcggcgcca ttgttctggc tactatcgtt ttatcatgag tgggttggta attggggtgt
     1021 tgcaatcatc ttactgacta tgacagtcaa gcttttattt ttcccattat cagccgctgg
     1081 ctatcgttca atggctaaat tgcgtcttgt aacgcctaag ttgcagcgca tacaggaaca
     1141 atacaagagt gatcgtcaac agatgcatca ggcgatgatg gaactttaca aagaagaaaa
     1201 aattaaccca ttaggtggat gtttacctat tttggtgcaa atacctgtat tcatcgcact
     1261 tttctgggtg attcttgcgg ctgtcgagtt acgatatgct ccgtttgcat tatggattac
     1321 tgatttatca tcacccgatc cgctttacgt catgccctta ttaatgggga tatcgatgtt
     1381 tatccagttt aagctaaatc ctaagcctac tgacccgatc caggctaagg ttctgcaaat
     1441 catgccaatt gcctttagca tatttttctt tttctttccc gcagggcttg tattgtattc
     1501 tttggtaaac aatattttat ctatcttgca acaatggcaa attacgcgtt tgtacggtca
     1561 accatcaagt aatgacgcat caacgaaaaa gaaagtaaag gagaagtaat agagagtatc
     1621 ggcgaatgag tattttatta agtaatgctc attcgtcggt tgaaattccg gtaataatat
     1681 tttttggtgt ttttcattaa ttagacatac taacgattag agtaaatgct tcatgtacag
     1741 cagtgacatc attgctgcta tcgcaacacc accaggacgt ggtgggatag gaattatccg
     1801 tatttctggt aacaatctca aagagatcac ccaacaattg ttaggggatc tacccaagcc
     1861 gcgctacgca agtttgaatt attttcttga tcaaaatcat gaagtaatcg atcatggaat
     1921 tgccatctat tatgttgctc cccattctta cacaggcgaa gatgttttag aacttcatgg
     1981 acatgggggt cctgctgtca tcaatttatt attgaagcga tgcttagcat taggtgctcg
     2041 cttggccgag cctggtgagt ttacattgcg tgcctactta aataataaaa ttgatctagt
     2101 gcaagctgag agcatagcgg atttaatcga tgctagcacg gctggtgcag cacgcagcgc
     2161 actgcgttct ttgcaaggaa aattttctga agcgattcac gaattagttc gcgcattgat
     2221 cgatttgcgc atacaggtcg aagcggcact ggattttcct gaagaggata tcgattggct
     2281 cgaaaccacg cacttaaaaa cacagttaaa tcaattatac gaaaaacttg agaaagtcta
     2341 tgtttcatca caacgtggca atttgttgag agaaggcgca aaaattgtat tgattggaca
     2401 acccaatgtc ggaaaatcga gcttattaaa ccgactggcc ggtgatgaag tggctatcgt
     2461 tactgaaatt ccaggcacca cccgcgatgc gattcaacag cttatccaga ttgatggcgt
     2521 tccattacac ataatcgata ccgcaggatt aagagaaacg gaagatattg tggaaaagaa
     2581 aggaattgaa cgcacctata ctgcgatgga acaagcggat ttagccttgc tgttgatcga
     2641 tagccaaaat gagttatctg aagccgatca aaatttattg agcaaactac ctgtaaatct
     2701 tcctgtcctt atcgttttta ataagattga tttgctgaat caaccagcga agagtgaaga
     2761 taaaaatggg tactcaatga tttatctttc agctaaaaca ggtgttggta tcgagatatt
     2821 atgtgaaaag atccttacat tagtcggttg gcgttcacat gtcgctggcg aaggcatatt
     2881 tatggctcgc cagcgacact tacaagcgct tgatcaagcg caaagacatt taagtaacgc
     2941 aatcgcctgg gcaagcagtg gaaaagcgat agaggtcctg gctgaagaat tacggcttgc
     3001 gcaatattcc ttatcatcaa tcgtcgggga attcagtgca gatgatctct tgggcgaaat
     3061 tttttcgcat ttttgtattg gtaaatagta aaaagaccac tagaaaaccg aaatttgttc
     3121 aattttaata actttaatat ggtttttctc acataagaat aattatccac ctgctatatt
     3181 aatccccagg gaaaaatggg atgatcgagt gcttcagcta gaggcttgaa ctgatggacc
     3241 gcggttattt taattgtcac gcaactgaaa tataggggga ttaatcatga aaaggtacct
     3301 taaacctgct gtacttgtaa tattttcagt attctttgga ttcaacttgc ttcacactca
     3361 tgcgaaagat gatttttctg aaattgttct tgtagatgga aaagttcaat ctcgcgtaga
     3421 gctagatcaa ggctggagcc aagaaatccg ccaattattt tggtttacca gccaaggttc
     3481 tcgcattatt ccctacaatt ggtttgcttg gttagaacag gcagataaca acagcttatt
     3541 tcgcaatgtt gaacatatgg agatgctgcg ctatcttccc atgaattcct cgaagctaaa
     3601 ccctgctgga ttgcccattg gattcgctct ggatactgat cacagcacag gaagaaaatg
     3661 ggtaggcctt acttgtgctg cttgccatac caatcaaatc gattatcaag gaacgagaat
     3721 gttaatcgag ggcgcaccaa cgcttgctaa tttcgttaaa ttttttgatg atttggttaa
     3781 tgcgctaaat aaaacaaatg ataatccaca aaaatttacc cgatttgcta aaaatgtatt
     3841 gggcaatcaa tactcagaaa caactgcaaa taagctccat caagagcttg accagatcgc
     3901 agctaaatta gcgcagcgtc ggatggttaa tcaattacca ccggattatc ctgaggattt
     3961 tacgagctat gcgcgtttag atgcgtttgg caatatacaa aatgctgcat ctgcttttgc
     4021 tttgcatgac ctaacgaata gaaatgcacc aaccgcacca gtttcctacc cttttttgtg
     4081 gggaacgcat caatctgatg ttgttcaatg gaatgggtct gcgcccaata caccgatcat
     4141 tggaccgctt attcgtaata tgggtgaggt agttggtgtt tttggagatt tggagattag
     4201 cgaagcacca atctggaaaa aattactcgg aatcaaagtg gagtataacg cgcatgtcga
     4261 tatccataat ttgggtaagt tagagttatg ggttaaacat ttgcgtgctc ccagctggtc
     4321 cgatggcaat atagaattgg cggtt
//
LOCUS       DAOQKQ010000198         4336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_11579_length_4336_cov_5.100444, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000198 DAOQKQ010000000
VERSION     DAOQKQ010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11579_length_4336_cov_5.100444"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PKZ49_11060"
     CDS             complement(<1..222)
                     /locus_tag="PKZ49_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HND37143.1"
                     /translation="MTYKLEFKKSALKEWEKLGHTIKDQFKKKLKERLKNPHVPSAAL
                     SGAENIYKIKLRQLGYRLVYSVEDKTVTVT"
     gene            complement(212..460)
                     /locus_tag="PKZ49_11065"
     CDS             complement(212..460)
                     /locus_tag="PKZ49_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002057569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HND37144.1"
                     /translation="MTHQILTEIAASISELKANPMKVVSSGNGMPIAVLNRNEPAFYC
                     VPAAAYEAMMELIDDTELLKLVKERIDEPTVTVSLDDL"
     gene            863..1726
                     /locus_tag="PKZ49_11070"
     CDS             863..1726
                     /locus_tag="PKZ49_11070"
                     /inference="COORDINATES: protein motif:HMM:NF013003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HND37145.1"
                     /translation="MTPEVIQTIAAYLAMAVPATVAISNIAKLIMEWLQQRHKIVEAQ
                     IQLSHQITTHYLDRALDPAAPLAIRHQLLRFLATPDLQGARLSTWAKAELERVGGIVD
                     ETNRAVASAEEGLRLAKNAADLERAEQKLVEAVRRQRGLLEPPTTPPVTAAALRAGLI
                     QEKKLNRLEMPGADLTNTKLVYRELRGANLKGANLSHSSLQGCDLRATDLSGALLIGT
                     TLYTADLRGANLTNSKLRSCNLQQARLEGADLRGAEIADCDLRATYDDSTQWPDAFDP
                     VQSGAVHTVSG"
     gene            1816..2160
                     /locus_tag="PKZ49_11075"
     CDS             1816..2160
                     /locus_tag="PKZ49_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37146.1"
                     /translation="MSIIEGYCSPISVAGGQNISFHISSQLNFRLEFVCLSEAETEKM
                     IIVGGIQNIFPAGVYPTPLRANETGCNWPSLLLITIPTFWNSGTSMELQWGQTRLFAR
                     NKSQLSKIQRAM"
     gene            2394..2744
                     /locus_tag="PKZ49_11080"
     CDS             2394..2744
                     /locus_tag="PKZ49_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37147.1"
                     /translation="MNFVEMSEQEIIEIANPIMDNLMDASTRIDHEAHVRDFTDRIKK
                     IVTKEHFQLVCKQYQNEKGFFAERKLVAVFKRPNSAAIVWKQYFTKAKGEFVAEMVLV
                     HQNGKFFCDHAMVF"
     assembly_gap    3120..3219
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3222..3698
                     /locus_tag="PKZ49_11085"
     CDS             3222..3698
                     /locus_tag="PKZ49_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HND37148.1"
                     /translation="MGLGAKKNGPNMALRAGGFFGIFQALMPFIGYWGGQSVLGWVET
                     YAHWIAFGLLTLIGAKMIYESTQEGVEETIARITNKMLLVLAIATSIDAMAAGFSLTL
                     LEVNPYVACLVIGVITFVFSVVGVYIGNRSGTWLESKAEIFGGIVLILMGIKFLIL"
     gene            3954..>4336
                     /locus_tag="PKZ49_11090"
     CDS             3954..>4336
                     /locus_tag="PKZ49_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HND37149.1"
                     /translation="MTTPAMNTICLWYNGNAQDAAQFYADTFPNSFIGAVYRAPGDFP
                     SGKEGDILTIEFTLMGIPCIGLNGGPAFVHNEAFSFQVATTDQAETDRYWNAIVDNGG
                     QESECGWCKDRWGVSWQITPIALMKA"
BASE COUNT         1151 a          905 c          997 g         1183 t
ORIGIN      
        1 ggtgactgtt acggtcttgt cttcaacgga ataaacaagg cgatagccga gttgtcttaa
       61 tttaatcttg tagatgtttt ccgcacccga caatgcagca gatggaacgt gaggattctt
      121 caatcgttct tttagtttct ttttgaattg atctttgatg gtatggccta gtttttccca
      181 ttctttgagc gctgactttt taaattccag cttataagtc atccagtgaa actgtaaccg
      241 ttggttcgtc tatacgctct tttaccagtt taagcagctc tgtatcgtca atcagctcca
      301 tcattgcctc ataggcggca gcgggcacac agtaaaaggc gggttcatta cgattcaata
      361 cggcaatggg cataccgttg ccactggaaa caactttcat ggggttagcc ttaagttcag
      421 agatactcgc tgcgatttct gtcagtattt gatgtgtcat agctttattt ttgagcagtc
      481 aaacaataag actgaattat aggtctccta aaaggtcttg taaagtttgc gattataatg
      541 ttttagatca ttgaaatgtg attggaaaaa tatcagaact aaccttaatc gctacaagct
      601 aaaataatcc taagatctag gatcaatcag cattgctcta tttttatcct gtgatggact
      661 cggaagcggg gggttgcttt ctgtgtgatt attcattcaa tttgtggcac taaacaagtc
      721 tgataaaatg acagctaaat tgacgattag ctgtgaatat aaggggatat catgaacaac
      781 tccatattag atttcattcg cgacgtaata tactgcgtca tttcggacgt ctaattctag
      841 ttaggcgtcg aaaacaacga tcatgacccc cgaagtaatt cagacaattg cggcatacct
      901 tgcaatggcc gtaccggcga ctgtcgcgat ctccaatatt gcaaaactga tcatggagtg
      961 gctgcaacaa cgtcacaaga tcgtcgaggc acagattcag ctcagtcatc agatcacgac
     1021 gcactacctc gaccgcgctc tcgatccagc cgcgcccctt gcaatccgcc atcagcttct
     1081 tcgcttcctt gctacaccgg acttgcaggg cgctcgacta agcacgtggg ccaaggccga
     1141 attggaacgg gtggggggaa ttgtcgacga gacgaatcgt gctgtggctt cagcagaaga
     1201 aggcctgcgt ctcgcaaaga atgccgccga tctcgaaaga gccgagcaga agctggtaga
     1261 agccgtacgg cgacaacgcg gtctacttga gccaccaacc acacccccag tcactgcagc
     1321 agcgctgagg gctggattga tccaggagaa gaagctcaat agacttgaga tgccgggagc
     1381 cgacctgaca aacaccaagt tggtgtaccg cgagcttcga ggagcgaacc taaagggggc
     1441 caacctttct cactccagcc tccaaggctg cgaccttcgc gccacggacc tctcaggggc
     1501 attactcatc ggaacgaccc tgtacaccgc ggatcttcga ggcgcgaact tgacgaactc
     1561 gaagctgcgc tcctgcaatc ttcagcaggc acgcctcgaa ggggctgatc tacgcggcgc
     1621 cgagattgct gactgcgacc ttcgggccac ttacgacgac tctacccagt ggccagatgc
     1681 attcgatcct gtgcagtccg gagcagttca tactgtatcc ggctaggcat ggagagtgaa
     1741 ccctgtcgac gcctaactct acgtagggtt tactcaatac atctcagttt taaacttgcg
     1801 atctaaggat acaacatgtc aattatagaa gggtattgca gcccgatctc tgtagctgga
     1861 ggacagaaca tttcatttca tatttcttcc cagttgaact ttcgactcga attcgtgtgt
     1921 ttatcggaag ctgagacaga aaaaatgata attgttggtg gaatccaaaa tatatttcct
     1981 gctggtgttt acccgactcc attacgcgca aatgaaacag gatgtaattg gccatcacta
     2041 ttgttgatca ctatccctac attctggaat tctggaactt caatggaact tcaatggggt
     2101 cagactcgat tgtttgcgag aaataaaagt cagttaagta aaatccaaag agctatgtga
     2161 gatcaatttc tgcttttatc ctttgatgaa cccagaagcc aaggtttgct tttattgtgt
     2221 agttattttt ttcaacttgt ggcactaaca agtttgataa aacaacagct aaattcgcta
     2281 ttggcggtaa taaggggcta tcatgaataa ttccgtatta aattacattt gtgatgaata
     2341 tattgtgcta tttcggatgc ctgattgtat gttagcactt gcaaggagta gtcatgaatt
     2401 ttgtagaaat gtcagaacag gagattatag aaatagcaaa tcccatcatg gacaatttga
     2461 tggatgcttc cacaagaatt gatcatgagg ctcacgtaag agatttcacg gatcgcatca
     2521 agaaaatagt tacgaaagaa catttccaat tggtttgcaa gcagtatcaa aatgaaaaag
     2581 gcttctttgc agagcgtaag cttgtagcag tattcaagcg tccaaattct gcagcaatcg
     2641 tttggaagca gtatttcacc aaagctaaag gcgaatttgt tgctgaaatg gttcttgttc
     2701 atcaaaatgg gaaattcttt tgcgaccatg caatggtgtt ttagcctacg tgctaactag
     2761 gtgttcaact ggacagtttt cagtggggta attgtttcgc aaatctctct cgaaccatag
     2821 gagatatgaa attgggtacc gatgccgtac tccccccaca ggaatttcgc catcatagat
     2881 tttcatatcg gtattttcaa tgggcagagt tgccgctgag atcaatgttc tctgcgatat
     2941 gatcaatgtt atgagcttca ttattgagag cataccatat aaccacgtac gttttttcga
     3001 agcaacacga tgatcgaagt cattattctt gcaatcgcgc tcagtatgga tgcctttgca
     3061 gtgtccatcg gtttgggtgc gaagaaaaat ggccctaaca tggcactgag agccgggggn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc catcggtttg ggtgcgaaga
     3241 aaaatggccc taacatggca ctgagagccg ggggattctt tggaattttt caagccttga
     3301 tgcccttcat cgggtattgg ggtggtcaga gtgttttagg ctgggtagaa acctatgcgc
     3361 attggatcgc ttttggactg ctgacgttga tcggtgcaaa aatgatttat gaaagcactc
     3421 aagaaggtgt ggaagaaacg attgcgcgca tcaccaacaa aatgctgctg gttctggcca
     3481 tcgctactag tattgatgcg atggctgctg gatttagcct gactttgttg gaagttaatc
     3541 cttatgttgc ttgcttggtc atcggagtga taacttttgt gttcagtgta gtcggtgttt
     3601 acatcggtaa ccgcagcgga acttggctgg aaagcaaagc ggaaattttt ggtgggatag
     3661 ttttgattct gatgggtatc aaattcctta ttttatagtc agaagggacc atcaacaggg
     3721 cctagcctgt tttagtatta cactaattgc aagctgattt ctattttgca aaaatatggc
     3781 acatcttctc cgtttgatgc tcatcaacga atcgtggcat attgcttaac acaagaataa
     3841 ccatcgatgg taatgaaatt tggtctgcac tgatttgaac ctcaaatctt tgataagaag
     3901 cacattaccg ccctaccaaa agattatatt tttgatctca atggagaaat gacatgacaa
     3961 caccagcaat gaatacgatt tgcctttggt ataatggcaa cgcccaagac gcagcacagt
     4021 tttacgccga cacttttcct aactcattta tcggtgcagt gtatcgcgca ccgggagatt
     4081 ttccgtcagg aaaggaagga gatatattaa ctatcgagtt caccctaatg ggaattccat
     4141 gcattgggct caacggcggc cccgcatttg tgcacaatga agcattctcg tttcaggtag
     4201 caactactga tcaagctgaa actgatcgct attggaacgc gatcgtcgat aacggtggtc
     4261 aggaaagtga gtgtggctgg tgtaaggata ggtggggagt gtcctggcag ataacgccaa
     4321 tcgccctgat gaaagc
//
LOCUS       DAOQKQ010000199         4151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_12517_length_4151_cov_5.612549, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000199 DAOQKQ010000000
VERSION     DAOQKQ010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12517_length_4151_cov_5.612549"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            247..1395
                     /locus_tag="PKZ49_11095"
     CDS             247..1395
                     /locus_tag="PKZ49_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HND37150.1"
                     /translation="MSNNPIIRWREAGEEKSARWQSEKSLQPPQQVKVIDDRMPADNA
                     YRLACEGTALLWRSDFQNAKQLLQAMARRIDKKRSTSKHLPAELSVADTFHRYRLMQS
                     QRAQILDKLLIPFQADHTIALPRAPDIRHACLEAYGPRNEPYVASLRELLGLIGAHEW
                     RKKGINIPVLDITIHPHYGVFSPVRNEYIQLIAQAALPKILTMQSVAFDIGTGTGVLA
                     VILAQRGVQRIVATDHDPRALDCARDNLLRLGLIEKLELLQTDLFPQGQAALIVCNPP
                     WIPARPASPMEHAIFDPENRMLRGFLQGLRDHLLPGGEGWLILSDIAEHLGLRSRAEL
                     LHMIDTAGLKIISKSDVKPHHPRVSDTSDPLHTARTRELTSLWQLAAK"
     gene            complement(1405..1734)
                     /locus_tag="PKZ49_11100"
     CDS             complement(1405..1734)
                     /locus_tag="PKZ49_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HND37151.1"
                     /translation="MNDIQQLTEILLKFRDERDWAQFHNAKDLALALNIEAAELLEVF
                     LWKAPEQANIESVKEELADVLTFALLLSEKYGLDVKQIVLEKLEKNALKYPVDKSKGS
                     AKKYTEL"
     gene            complement(1747..2001)
                     /locus_tag="PKZ49_11105"
     CDS             complement(1747..2001)
                     /locus_tag="PKZ49_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HND37152.1"
                     /translation="MNLIFSTQAWEDYLYWQKTDKSTVKRIHGLIKAIQQKPFEGIGK
                     PEPLKHGLAGYWSRRIDNEHRIVYKILNTDLFIAQLRYHY"
     gene            complement(1998..2249)
                     /locus_tag="PKZ49_11110"
     CDS             complement(1998..2249)
                     /locus_tag="PKZ49_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HND37153.1"
                     /translation="MDAISYTTARANLAKTMERVCNDHSPVIITRKREASVVMISLED
                     YQAMEETVYLLRSPANARRLLESIAELEAGKGTVRQLAE"
     gene            complement(2406..2630)
                     /locus_tag="PKZ49_11115"
     CDS             complement(2406..2630)
                     /locus_tag="PKZ49_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2835 family protein"
                     /protein_id="HND37154.1"
                     /translation="MHQRVRVVLKIPAYQLELYYQGVVDTVAAETTDGRIVHFPANVL
                     RSVVQSHGVYGTFELVFDENNKFVSISSTS"
     gene            complement(2651..3391)
                     /locus_tag="PKZ49_11120"
     CDS             complement(2651..3391)
                     /locus_tag="PKZ49_11120"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HND37155.1"
                     /translation="MEKIRLSKLMSERGICSRREADRFIELGWVFVDGERISELGIKI
                     FPTQKIALNKAAQAQQTDLVTILLNKPIGYVSGQPEPGYQPAVTLIKPENLFSPKQAM
                     QPAKRFQPWHLKKLAPAGRLDIDSQGLLVLTQDGRIAKQMIGENSRIEKEYLVRVEGV
                     LPVAKLALLNHGLSLDGQALKPAQVSWQNRDQLRFILNEGKKRQIRRMCELVGLKVTG
                     LKRVRIGKVKLGNLPEGQWRYLENGEKF"
     gene            3622..3975
                     /locus_tag="PKZ49_11125"
     CDS             3622..3975
                     /locus_tag="PKZ49_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37156.1"
                     /translation="MKTLLPFACIYILLIAPTGFLMGQLFFGHFSIAASLAGSAGLIC
                     AFAGFGVIGGAIKARSIAFWSGLFALIGVAFDAADYYLNYAIPGNYYAWGLIGPYCCA
                     IIFIAYVSRSLMAVK"
BASE COUNT         1098 a         1039 c          814 g         1200 t
ORIGIN      
        1 agagatttgt atcaaagtaa ccgaagccgt gctgacttat gttgccattg atactgatcg
       61 acaccctcgc cctgttccgc ctgaataaga ccaaccaata ttagttttat agtgagttta
      121 aatcgcttat ctttggtttt ggcgctagat tagcagttac aatccgtagt ctagcgccat
      181 ttctatttct aattattcag cactcttatg cccagccacc ctgccaataa caccaccagc
      241 accctcatga gcaacaatcc gattatccgc tggcgtgaag ccggagaaga aaaatctgcc
      301 cgctggcaaa gtgaaaaaag cttgcaacca ccgcaacagg taaaggtcat tgacgatcgt
      361 atgcctgctg ataatgctta ccgcttggcg tgtgaaggca cagcattact atggcgcagc
      421 gattttcaaa atgccaagca gttgctccaa gccatggctc gccgcattga caagaaaaga
      481 tctacatcga agcatctacc agccgaattg tcagtcgccg atacttttca ccgttatcgc
      541 ctgatgcaat cacaacgtgc gcaaatactc gataaattgc tgatcccgtt tcaagcggat
      601 catactatcg cgttaccccg tgcaccagat attcgccatg cctgcttgga agcatacggc
      661 ccccgtaatg aaccgtatgt tgcttcacta cgcgaactgt tgggtttaat tggcgcacac
      721 gagtggcgca agaaaggtat caatatcccc gtactggaca tcaccattca tccgcattat
      781 ggggtatttt ccccagttcg caacgagtat atccagctca tcgcccaagc agcgctacct
      841 aaaatactaa ccatgcagtc agtcgcattc gatattggta ctggcaccgg cgtattggct
      901 gtgatattag cgcaacgggg tgttcaacgc attgttgcaa ccgaccatga tccacgcgcg
      961 ctcgattgcg cacgcgacaa tctgttacgt ttgggtttga ttgaaaaact cgaattactg
     1021 caaaccgacc tttttcctca ggggcaggca gcattgattg tctgcaatcc accgtggata
     1081 ccagctcgac ccgcttctcc catggaacac gccatattcg acccagaaaa tcgcatgctg
     1141 cgaggattct tacaaggtct gcgcgatcat cttcttccag gaggtgaagg ttggctgatc
     1201 ctatcagata ttgccgaaca cctgggattg cgtagccgtg cagaactact acacatgatc
     1261 gatactgcag gcctaaagat catatcgaaa tccgacgtca aacctcatca tcctcgggtc
     1321 tccgatacca gtgatccact gcacacagcc cgcacaagag aattgacatc cttatggcaa
     1381 cttgccgcaa aatagccaca acacctacaa ttccgtatat tttttagcgc ttcctttaga
     1441 tttatccact ggatatttca aggcattttt ctccagcttc tctaatacaa tctgtttcac
     1501 gtctaaacca tatttttccg acagcagcaa agcaaatgtc aacacatcag ccaactcctc
     1561 ttttacgctc tcgatattcg cctgttccgg tgctttccac agaaaaactt ccaataattc
     1621 tgctgcctcg atattcagcg ctagcgccaa atctttcgcg ttatgaaact gtgcccaatc
     1681 acgctcatcg cgaaacttta gcaaaatctc ggtaagttgc tggatgtcgt tcacagaaaa
     1741 gaacctttaa tagtgataac gaagttgtgc gatgaacaaa tcagtattca aaattttata
     1801 aactattcta tgttcattat caatccgcct tgaccaataa ccagctaacc cgtgttttag
     1861 cggctctggt ttgccaatcc cttcaaaagg tttttgctga attgccttga ttaaaccatg
     1921 aattcttttt actgtggatt tatcagtttt ttgccagtaa agataatctt cccaagcctg
     1981 agtagaaaaa atcaggttca ttcagccaat tgccgaacag tgccttttcc agcttccagt
     2041 tcagcaatag attccaacaa tcgccgtgca ttagcaggag aacggagcaa atacacggtt
     2101 tcctccatcg cttgataatc ctctaacgat atcatgacaa ccgacgcttc tcgcttacga
     2161 gtaataataa caggagaatg atcgttgcaa accctttcca ttgttttagc taaattagca
     2221 cgtgctgttg tataactaat cgcatccatg acaaatacct ctactggtac agaattaagt
     2281 acaatcagtt taacacgcat acttgatggg tcaactttaa tacttttaac ctctaactga
     2341 tactattccc cagcttatta tgttcttccc acttctttat gttcgcaaaa ttattcagca
     2401 ctccctcaac tggtgctact gatcgataca aatttattgt tttcatcgaa taccaattca
     2461 aacgtaccgt atacaccatg actttgcacc accgagcgca gcacattggc tggaaaatgc
     2521 acaatgcggc cgtcagtagt ttcagccgcg acggtatcga caaccccttg gtaataaagc
     2581 tcaagttgat acgctgggat tttcagcacg actcgtacgc gttgatgcat gcaatgacct
     2641 caaaatacca ttaaaacttt tcaccatttt ccagatagcg ccattgacct tcgggtagat
     2701 tgcccagctt cactttacca atgcgcactc gtttcaaacc ggttacttta agaccaacca
     2761 gctcacacat acggcgaatt tgacgttttt ttccctcgtt caaaatgaag cgcaattgat
     2821 cgcggttttg ccagctcacc tgtgctggtt ttagggcctg cccatccaaa ctcaaaccat
     2881 gattcagcaa tgcgagttta gcaaccggca gcacaccttc aactcgcacc aaatattcct
     2941 tttcgatgcg tgaattctca ccgatcattt gcttggcgat gcgaccatct tgcgttaata
     3001 ccagtaaacc ttgcgaatcg atatcaaggc gaccggcagg ggccagtttt tttaagtgcc
     3061 acggctgaaa tcgcttagcc ggttgcattg cttgcttggg tgaaaacaga ttttccggct
     3121 tgatcagcgt cacggctggt tgataacccg gttccggctg cccagacaca taaccgatcg
     3181 gcttgttgag cagaatcgtc actaaatcgg tctgttgcgc ttgtgctgct ttattcaagg
     3241 caattttctg tgtcggaaat attttgatgc caagttcgga gatacgctcg ccatcgacaa
     3301 atacccaacc aagctcgata aaacgatcag cttcgcggcg tgagcagatg cctcgttccg
     3361 acatcaattt ggataaacgg attttttcca tggcgaggaa tgataagtta gatttacccg
     3421 cattataaag gtaatcgctt gagctttttc actccatctc gaggatcatt tattgaaacg
     3481 atcgaatcaa tactacccct ctgaataaga ctaatcagta ttttgtactt cataaaataa
     3541 cctatctttt gttttttctc gagccatcgt tgttttattt tatcggttaa gctgacgcct
     3601 tcccagataa aatcaaccac catgaaaacg ctcttaccgt ttgcttgtat ctatatctta
     3661 ttgatcgcgc ctacaggatt tttgatgggt caattgtttt ttggccattt ctcgattgca
     3721 gcttcgcttg ctggcagtgc aggattaatt tgtgccttcg caggatttgg tgtaataggc
     3781 ggagcgatta aagcaagatc gatcgcgttt tggtcgggtc tgttcgcatt gatcggcgtg
     3841 gcattcgatg cagcagatta ctatcttaat tatgctattc ccggtaatta ctatgcctgg
     3901 ggattaattg gcccctattg ttgtgcaatc atttttatcg cttatgtgag tcgctcgctc
     3961 atggctgtaa aatgaaggga ttcctaaaaa ctaactgccc catcatcggc gatgaggcag
     4021 ttaggcatgt ctatgtattt atacaacaag attaacttag caagttaatc ttaacgctat
     4081 tatttcttac ctttgtcatg atcatcgtct ttgtcgtgct tatgatcttt atcgccgaaa
     4141 tatttcaagg t
//
LOCUS       DAOQKQ010000200         4146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_12537_length_4146_cov_6.820093, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000200 DAOQKQ010000000
VERSION     DAOQKQ010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12537_length_4146_cov_6.820093"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(385..2325)
                     /locus_tag="PKZ49_11130"
     CDS             complement(385..2325)
                     /locus_tag="PKZ49_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37157.1"
                     /translation="MASNAISSTSLHALSLGLNFIPVPQLPTSTTVSSYLLSQFEDYK
                     RRLNIQHFFQLKPSRSTTSNSFRIRSTWQPSEEDFRPSQYSPILKQYLSTVQSRLQQL
                     PSSHQLSTTLKTFLSNYHNPQWLKPALRSLRQNMDIIVTEADKNMGIVVMDRDAYQKE
                     GLRQLSCSNTYRHITNIMIGIFMQPYGKLRTILNKYDRLWTHDFRLKSKKLSSLAEYL
                     LQLESQHKIESKLSSLAAKFYLLMKMHKSPVVGRPIVSTVNSFTYHTSVFVDAELKSL
                     MKLIPSYLESSQQLIYNLETLTLPPDCYICCADIESLYPNIPIDIGMQFVKSAIQRLS
                     WISPDVHCPLRDDNHYLDFILELMEWVLRNNIFKFGNSWYQQLQGTAMGTPLAVPFAC
                     LFVAELEHRISSFTPIYYKRYIDDIFMITTSKEEAELFINRFNSVVPTIRCGSVTIDF
                     TTGIFLDIEVYKGSRFCTANLLDVRIYQKVQNRYLYLAPNSFHRRDIFKSTIISELNR
                     YRLCCSNDVEFFSIKCLFFQRLVARGYNPDYLISLFPFHSTRSQLLQNISLRFSIDSK
                     KQSTTSSSLSLPIFKSLNTYETTQLSISKLISIPLDLQNAINDDDFMKQLFSQSPITC
                     FCNADTAYKLIGNARKTLHENE"
     gene            complement(2445..3716)
                     /locus_tag="PKZ49_11135"
     CDS             complement(2445..3716)
                     /locus_tag="PKZ49_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37158.1"
                     /translation="MDSDPNQHPPPANELQLVDDSRNPKRQRIDNQFMLTTNLNRDTG
                     PIMDLDDDPNSLSTATASATAAHPTTPGPATAEEALHSKLLKDIFQVIINKRVNKYGS
                     DRYEHYNRQVALRSLSNHATKNTFPKDLCFTVQTGNPYKKSTPNRDALLQEEQNILMD
                     AKKRILHQRIHTAESDLQRLATTCEQYFYLDIIVNDFKDEFKDAIVLTDAQCSELKNA
                     YNLCLINKRTNMDNLFKRKEEQYQKHIAKKQKTTPISATLNEQQFKSSLIDLMKDYLQ
                     QQQAKDKKSSIPAPSTDHTKAREKTNKKHKQSNSSKPVSSSSSNTSNNKNHSTNNDKK
                     KNVQKQQQQPKSRQVKFNDRTSTKPTYADKVKSYNKNRVIIEHLDDSDTEDDDDGFIT
                     VTNKKRRKNAKNDSATDPRTVPKRPYIRKKY"
BASE COUNT         1255 a          616 c          929 g         1346 t
ORIGIN      
        1 gggttaggtt agggttaggg ttaggtaggg gttaggttag ggttaggtta gggttaggtt
       61 aggttaggga tagggttagg gttagggtta ggttagggtt agggttaggg ttagggttta
      121 tagggagaag tcagagtcag ttgcgccgtg gcgaagcgaa gcgtagcagg cgcgcggagc
      181 gtagcggagc catggcgagc gtagcgagcc taaaatttga tttttggcgc attcaatcat
      241 atctgattga tatgttgata actgaaatac tgatgaatta ctatgtttct gctttgacga
      301 gaatgtcata gataattcga tagagtgagc tgcggcaata aaagaataag tgataatagt
      361 agtaagttga aattgggtcg ctactcattc attttcatga agagtttttc gggcattgcc
      421 aattaattta taagctgtat ctgcattaca aaagcaagta attggagact gagagaataa
      481 ttgtttcatg aagtcatcat cgttgattgc attttgcagg tctagcggga tactgataag
      541 cttagaaatg gataactggg ttgtctcata tgtgtttaat gatttaaaaa tgggaagcga
      601 taatgaagaa gatgtagtgg attgtttctt gctatcgatg gaaaatctga gagagatatt
      661 ttgtagaagt tggcttctag ttgaatggaa cggaaataac gatatcagat agtcgggatt
      721 ataacctctt gcaaccaaac gttgaaaaaa cagacattta atgctgaaaa actcaacatc
      781 gttggaacag cataagcgat agcgattcag ttcagatata atggtacttt taaatatatc
      841 tcggcgatga aaagaatttg gggcaagata gagataacga ttttgtacct tttgataaat
      901 tcgtacatca agcagattag cagtacaaaa tcgagatcct ttgtatacct caatgtccaa
      961 aaatattccc gtcgtaaaat cgatagttac agatccacaa cgaatagtgg gaacgacaga
     1021 attaaagcga ttgataaata attctgcttc ttcttttgag gtcgttatca taaaaatatc
     1081 gtcgatgtaa cgtttatagt agataggtgt aaacgatgaa atacgatgtt ccaattccgc
     1141 gacaaaaaga catgcaaatg gtactgctaa aggagttccc atcgcagttc cttgtagttg
     1201 ctgataccaa gaattgccaa acttgaatat gttgtttctc agaacccatt ccataagttc
     1261 gagaataaaa tctaaataat ggttatcatc tcgaagtgga caatgaacgt cgggtgatat
     1321 ccacgaaagg cgttgaatag cactttttac aaactgcata ccaatatcaa ttggaatatt
     1381 gggatacaga ctttcaatgt ccgcacaaca gatatagcaa tccggtggaa gtgttaaggt
     1441 ttccagattg tatattaact gttgactgga ttccagataa gatggaatta gtttcattaa
     1501 cgattttaat tccgcatcta cgaatactga tgtatggtac gtaaatgaat taactgtcga
     1561 aacaataggt ctgcccacca caggtgattt gtgcatcttc attagtaaat aaaattttgc
     1621 agctaatgaa ctaagtttcg actcgatttt atgttgagat tccagttgaa gaagatattc
     1681 agcaagtgat gataatttct ttgatttcaa acgaaaatca tgtgtccata agcgatcata
     1741 tttgttaaga attgttctga gcttgccata tggttgcata aaaataccaa tcatgatgtt
     1801 cgtgatgtgt cgatatgtgt ttgagcaaga taattgacgc aatccttctt tttgataagc
     1861 atcacgatcc atgactacaa ttcccatatt tttatcagct tctgtaacga taatgtccat
     1921 attttgacga agcgagcgca gagcaggttt aagccactgt ggattgtgat aattcgataa
     1981 aaatgttttc agggtagttg atagttgatg tgatgatgga agttgttgca agcgagattg
     2041 tacggtcgat aaatattgct taaggatcgg agaatattga gacggtcgaa aatcctcttc
     2101 cgaaggctgc caagtactac gaattctgaa tgaattagaa gttgttgatc tacttggttt
     2161 tagctggaaa aaatgctgaa tattcaacct gcgtttatag tcttcaaatt gtgatagtag
     2221 ataagacgat acggtagtag aggttggaag ttgaggtact ggaataaaat taaggccaag
     2281 agatagagca tgaagtgaag tagaagagat tgcattggaa gccaaataat gaataccatt
     2341 gttagtaata gttggtatcc aattatgtct taacttccaa gctaaattga aaaatggatg
     2401 aagataatta catcgatgtt ttcgcggtcg aaaacgtcgt gcaactaata tttctttcgt
     2461 atgtatggac gtttggggac agttctggga tcggttgcag agtcgttttt tgcgttctta
     2521 cgacgttttt tgttagtaac tgtaataaat ccatcgtcat cgtcttctgt gtcactgtca
     2581 tccagatgtt cgataataac acgatttttg ttgtacgatt tgaccttatc tgcataagta
     2641 ggtttcgtag atgttcggtc attgaacttc acttgtcttg acttgggctg ctgctgctgt
     2701 ttctgtacat tcttcttctt gtcattattg gtagaatgat ttttattatt actagtatta
     2761 gaagaagaag aagagactgg tttcgacgag tttgattgtt tgtgtttctt gttcgtcttt
     2821 tcacgtgcct ttgtatgatc agtagaaggt gcagggatag aagacttctt atctttcgct
     2881 tgttgttgct gtagatagtc cttcataaga tcaataagac tgcttttaaa ctgttgctca
     2941 tttaacgttg cagatatagg cgttgttttc tgctttttcg caatatgctt ctggtactgt
     3001 tcctccttgc gtttaaataa gttgtccatg ttggtacgct tgttgattag gcaaaggtta
     3061 tatgcattct tcaattcaga gcattgggcg tctgtaagaa caatagcatc tttgaactca
     3121 tccttgaagt cgttcacgat tatatccaaa tagaagtact gctcacaagt tgtagcaagg
     3181 cgctgaaggt ccgattctgc tgtgtggata cgttgatgta agattctctt tttagcgtcc
     3241 atgagaatgt tctgttcttc ttgcaacagc gcatcacggt tcggagtaga cttcttgtaa
     3301 ggattaccag tctgtacagt aaaacataaa tctttgggga aagtattttt tgtggcgtgg
     3361 ttactaagag aacgtaaggc aacttgccta ttatagtgct catagcgatc tgagccatac
     3421 ttgttaaccc gtttgttaat aataacttga aaaatatctt tcaagagttt gctatgcaaa
     3481 gcttcttctg cggtggcagg tccaggtgtg gtcggatggg ccgctgttgc tgaagctgta
     3541 gcagttgaca atgagtttgg atcatcgtca aggtccataa taggaccagt gtcacgattc
     3601 aaattagtcg ttagcatgaa ttggttgtcg attctctgac gtttgggatt gcgagaatca
     3661 tcaacgagct gcaattcatt ggctggtggg gggtgctgat tcgggtcgga atccatttga
     3721 aaaccgtcaa gaaaaggcga aaaataaatt gatttctcct taaaatgaaa cggttatact
     3781 cttgttgaag tgtttgttaa tcacaaatta tagattacac aactagtttc gtatactatt
     3841 gtatactcat caggtatatc tatgaattca attgtgattt tgataaaaat aacaagagtg
     3901 aaaatgagac aaaacgcgtt tttgattcca aattctgaat tccgcttgat tcctcttgat
     3961 tccaagcgtt tcactgaata aataatgtaa ataatataaa tatataaata tattaaatac
     4021 aataatataa atatataaat aaataaatat aaatatatta atataagtag caaataaata
     4081 agctgacaat agtttggata tcagtttagg gatcaggtag ggtaacagtt acactggttt
     4141 ggatat
//
LOCUS       DAOQKQ010000201         4077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_12906_length_4077_cov_5.754600, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000201 DAOQKQ010000000
VERSION     DAOQKQ010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12906_length_4077_cov_5.754600"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /gene="maeA"
                     /locus_tag="PKZ49_11140"
     CDS             complement(<1..928)
                     /gene="maeA"
                     /locus_tag="PKZ49_11140"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate-decarboxylating malate
                     dehydrogenase"
                     /protein_id="HND37159.1"
                     /translation="MKQLYGKSLLDDPTLNKSTAFTREERKRFGLSGLLPYRVTDIYT
                     QTARVLNNMRSKHSAIEKYIFLNSLLERNQCLFYRTLIDHIEEILPLVYTPTVGEACK
                     QFGHIFRKPQGFYITPEDKGEIASILGNWPETDIRIIVVTDGERILGLGDLGANGMGI
                     SIGKTALYVACAGLHPENCLPVMLDVGTNNQALREDPFYMGYPHPRITGDEYLSLVDE
                     FVQAVQTRFPDAVIQFEDFHNQHAFQLLDRYRESVRCFNDDIQGTAAVTLSGIYTSCH
                     ITQKRFTDLNIMLLGTGSAASGIAGLLVSAFCA"
     gene            1180..1605
                     /locus_tag="PKZ49_11145"
     CDS             1180..1605
                     /locus_tag="PKZ49_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HND37160.1"
                     /translation="MTTLQSIPSPNPDLYTEEEISNLVHQFYAKARKDPDLGPIFEGH
                     VIDWDAHFVQMTNFWSAQLRGTNRFRGAPMPKHIALPALNADLFKRWLALFKETTQEL
                     GNPTLKHHADMVATFIAGRLWLGYQMSNYPDQPATSLAV"
     gene            1696..2637
                     /locus_tag="PKZ49_11150"
     CDS             1696..2637
                     /locus_tag="PKZ49_11150"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HND37161.1"
                     /translation="MQVFRSTDTPHKIPLALTIGNFDGVHLGHQAMLEELKQAAKRLN
                     IATCIMIFEPHPREFLMPGQSPTRLTSLREKLEIFAQLNIDRVQICHFNHAFAQISAQ
                     TFITDILMQQLNVRWMLVGDDFRFGAKRVGDVAMLRAYAQQTGQFNVEVMADFSINHI
                     RVSSTAIREALSAGNMALAQQLLGRPYSMSGRIVNGDKLAKQLGFPTANIHFKHSHPP
                     LRGIFVVEVISRENSNLPKVMQGVASLGVRPTTHASDKLILEVHLFDFDQEIYGQHLQ
                     VNFLHKLRDEEKYADLEKLIAQIKQDIKNAKHYFMLH"
     gene            2671..>4077
                     /locus_tag="PKZ49_11155"
     CDS             2671..>4077
                     /locus_tag="PKZ49_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HND37162.1"
                     /translation="MTDYKNTLNLPDTPFPMRGDLAKREPGMLKAWQEKKLYQKIREI
                     SQGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIKSKTLAGFDAPYVPGWDCHGLPI
                     EHQIEKKHGKHLPADQVRTLCRAFAQAQIERQKADFIRLGVLGDWDNPYLTMNYQTEA
                     DIIRALGKIYQNGHLYQGQKPVNWCIDCGSALAEAEVDYENKTSPAIDVGFEVVADES
                     AAQTLTNIFSSAIPEGRKCYAVIWTTTPWTLPANQAVCVHPDFDYELVDTPRGLLILA
                     AQLKEACLTRYQINGETLARCKGSALEHLVLHHPFDTRTVKIICGKHVTLEAGTGLVH
                     TAPAHGLDDYFIGQQYSLPSDSPVGNDGKFTQPTPLVGGMFVWKANDVVIESLQQSQH
                     LIHSEKIEHSYPHCWRHKTPIIFRSTPQWFIGMNKPQGANMDQYESLRELAIKAVDQT
                     TFYPAWGRARLEAMIKNRP"
BASE COUNT         1159 a          899 c          925 g         1094 t
ORIGIN      
        1 ctgcgcaaaa agcggacacc agcaaccctg caattccgct cgctgcagat ccggtgccga
       61 gcaacatgat attcaaatcg gtaaatcgct tctgcgtaat gtggcatgaa gtataaattc
      121 ccgagagcgt gacggcggca gtgccctgga tatcatcgtt aaagcaacgc acagactcac
      181 gatagcgatc caatagttgg aaggcatgct ggttatggaa atcttcaaat tgaatcaccg
      241 catcagggaa tctggtctgc accgcttgca caaactcatc gaccaaggat aaatattcat
      301 ccccagtaat acgagggtgt ggatatccca tataaaaagg atcttcacgc aaggcttgat
      361 tattggttcc aacatcgagc ataacgggca aacagttttc gggatgaagg cccgcacatg
      421 cgacatacaa cgctgtttta ccaatggaaa ttcccatccc attggcacct agatcgccaa
      481 gcccgaggat tcgttcacca tcggtaacga caatgatgcg aatatcggtt tcaggccagt
      541 taccgagtat cgatgcgatt tctcccttgt cttcgggagt gatataaaac ccttggggtt
      601 ttctgaaaat atgaccaaac tgcttgcaag cttctccaac ggtaggtgtg taaacgagcg
      661 gtaggatttc ttcaatgtga tcgatcaggg ttcggtaaaa caaacattga ttgcgctcaa
      721 gtaagctgtt caaaaaaata tatttttcga tggcgctatg cttgcttcgc atattattga
      781 gtacgcgcgc ggtttgggta tagatgtcgg taacacggta aggtaataag ccgcttaacc
      841 cgaagcgctt ccgttcttcg cgggtaaatg cagttgattt attgagggtt ggatcatcta
      901 acaaggattt tccgtacagt tgcttcatcc ttttctctcc gcataaaatg ggctgcaaat
      961 gcaaattgcg aaatggagtg cgtggggatt aaccatccat ccaatacgct agtctgtcgt
     1021 tagacaattt tgatagtaat cgataaacta gcaggcaaag gaaacaatta aacgattcac
     1081 gtatcgtcac atatctagtg cgataagtcg aggttttcca aaatgactca gcaatcccat
     1141 taaccgattt attatttgat aactgttttt tttctttata tgacgacact gcaatcgata
     1201 ccttctccta atcctgatct ctatactgag gaagagattt caaaccttgt tcatcagttt
     1261 tatgctaaag caagaaagga tcctgattta ggtcctatct ttgaaggtca tgtcatcgat
     1321 tgggatgcgc attttgttca aatgacaaac ttttggtcag cgcaattgag gggaaccaat
     1381 cgtttccggg gtgctcccat gccaaaacat attgcacttc ctgcactgaa tgcggatttg
     1441 ttcaaacgct ggttagcgtt gttcaaagaa acgacgcaag aactcggcaa cccaaccttg
     1501 aaacatcatg cggatatggt tgctaccttt atcgcaggtc ggctatggtt aggctaccaa
     1561 atgagtaatt atcccgatca acccgcgaca tcgctcgctg tttagcaggc agctagaaat
     1621 tgtgtctaat agggggctag atagcgacca gtctagggag aaacgaaaag cgctggcttt
     1681 atcttagcag ttaccatgca agtttttcgt agtactgaca caccgcataa aattccactt
     1741 gcattgacga ttggtaattt tgatggcgtc catttaggcc accaagccat gttggaggaa
     1801 ctcaagcaag ctgcaaagcg gttgaatatt gcaacttgca ttatgatttt tgagccgcat
     1861 ccgcgtgaat ttctaatgcc cggtcaatcg ccgactcgat tgaccagctt gcgtgaaaag
     1921 ttggaaattt ttgcgcaatt aaatattgat cgcgtccaaa tttgccattt taaccatgca
     1981 tttgcgcaaa tctcggcgca aacatttatt actgacatat taatgcagca attaaatgtt
     2041 cgctggatgt tagtcggcga tgattttcga tttggtgcta agcgcgttgg tgatgttgcc
     2101 atgctaaggg cctatgcgca gcaaacggga cagttcaatg tagaagtcat ggccgatttc
     2161 tcgattaatc atatccgagt ttccagtact gcgattagag aagccttatc agctgggaac
     2221 atggcacttg cccagcaatt attgggccga ccctatagca tgagcggtcg catcgtcaat
     2281 ggagacaaat tagcgaagca gcttggtttt ccaaccgcga atatccactt caaacatagt
     2341 catccaccct tgcgcggtat ctttgtagta gaagtcatta gtcgagaaaa ttcgaacttg
     2401 cccaaagtga tgcagggcgt ggcaagcttg ggtgtcaggc caacgacgca tgcttctgat
     2461 aagttaattt tagaagttca cttgtttgat tttgatcaag aaatttacgg tcaacattta
     2521 caagtcaatt ttttgcataa gctccgagat gaagaaaaat atgcagacct cgaaaaattg
     2581 attgcacaaa tcaaacaaga cattaagaat gcaaaacact attttatgct tcactaacct
     2641 ttgccttgca tcaatatagg gctctttttc atgactgact acaaaaatac actgaatcta
     2701 cccgacacac ccttcccgat gcgaggcgat ctggctaaac gtgagccagg gatgctcaaa
     2761 gcctggcaag aaaaaaagct ttatcagaaa attcgtgaaa ttagccaagg aagaccaaag
     2821 tttattctgc atgatgggcc accctatgca aatggtgata ttcatattgg tcatgcggtc
     2881 aataaaattc tcaaagatat cattatcaaa agcaaaacct tagccggttt tgatgcgccc
     2941 tatgttccag gctgggattg ccacgggttg ccgatcgaac atcaaattga gaagaagcac
     3001 ggcaaacatt tacctgccga tcaagtccgc acgctgtgcc gagcgtttgc gcaagcccaa
     3061 atagaaaggc aaaaagccga ttttatccgc ttaggtgtcc tgggtgattg ggacaatcct
     3121 tacttgacga tgaattatca gactgaggct gatattattc gggcgctagg taaaatctac
     3181 caaaacggcc atctctatca aggtcagaaa cccgtcaatt ggtgtatcga ttgtgggtca
     3241 gcgctcgctg aggcagaagt cgattacgaa aacaaaacat cccctgcaat tgatgtggga
     3301 tttgaggttg ttgctgatga atctgcagca cagacgctaa cgaatatttt ctcaagcgct
     3361 attccggaag gtagaaagtg ttatgccgtc atttggacca ctacaccctg gacgttgcca
     3421 gccaatcaag cggtttgcgt gcatcctgat tttgattatg agttagtcga tacaccgcgc
     3481 ggattgctga ttttagcagc acagctaaaa gaagcatgtt tgacgcgata ccaaatcaac
     3541 ggggaaacat tggcccgttg caaaggcagt gcgcttgagc atttggtttt gcatcaccct
     3601 tttgatacgc gaacggtgaa aattatctgc ggtaaacatg taacgctgga agccggtacc
     3661 ggtttggttc ataccgcacc tgctcatggt ttggatgact atttcatcgg acagcaatac
     3721 agtttaccca gtgatagtcc cgtggggaat gatggtaaat tcactcaacc gactccattg
     3781 gtcggcggta tgtttgtttg gaaagctaat gatgtcgtga ttgagtcgct ccaacaaagc
     3841 caacatctca ttcattctga gaaaattgaa catagctacc cccattgttg gcgccacaaa
     3901 acaccgatta ttttccgttc aaccccgcaa tggtttatcg gtatgaataa gccgcagggt
     3961 gcgaatatgg accaatatga atcactgcgt gaattagcga ttaaagctgt cgatcaaacg
     4021 acattttacc cagcctgggg aagagcacgc ttagaagcga tgatcaagaa tcgcccc
//
LOCUS       DAOQKQ010000202         4065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_12963_length_4065_cov_5.104738, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000202 DAOQKQ010000000
VERSION     DAOQKQ010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12963_length_4065_cov_5.104738"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..2392
                     /locus_tag="PKZ49_11160"
     CDS             74..2392
                     /locus_tag="PKZ49_11160"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HND37163.1"
                     /translation="MSKIQQTNSELLEEIQRLRAELAAVKWMTEKRAQELYMSEERFS
                     LAMRGANDGLWDWNLETDEIYYSPRWKSMLGYEAHQFANNLDTWASLVHPDDKAIALA
                     KTQDYLAGRTTSLEIEMRMRHKAGHDVFVLSRGFLVRRDSDGKPIRLVGTHVDITERK
                     KSEAFNNQHAEILEMIAVGRPASEIYDAIALMYESRHPGLRCSMLELSGNILLHGGAP
                     SLPKAYCEAVHGLRNGPNVGSCGTSTFTGKRVLVENIETDPKWADIKHFALPHGMRCC
                     WSEPIKNSSGKVLGAFGMYYNHPALPNEQELKDLISAARLAGIVMERDQAQKRIRALA
                     YTDELTGLASRASFIQHMEEIMKTSDRYNRRFGLLYIDLDNFKWINDSLGHDAGDALL
                     KTIATRLRNTCRDTDFIARLGGDEFCVLVEEIEADYAANVAKRCLAAIAEPIVIYSRN
                     LTPGCSIGIAYYPDDGNNPAALLKAADTSLYTAKSNGKNQYAFYCTELTRKAHYRFQI
                     EQNLKEAIATQQLTLVYQPQVNVITGEIFGVEALSRWHHPQIGSIPPSDFIATAERIG
                     MIKSLTEWVLRTACQQLIVWQTEGFHRLRMAVNISPSLFLETEFVSLVQRIVQETGIK
                     PSQLKLEVTEGVVQTDPRNLAIFQDLKALGVLLAIDDFGSGYSSFASLNHLKTDYLKI
                     DKYFIDKMITDQKAFTLVNTMIEMGHKLGYGIIAEGVETLEQLSILRSLNCEIVQGYL
                     FSQPVSAAQLSVLLQSDKHKMILDQCRVNKIA"
     gene            2490..>4065
                     /locus_tag="PKZ49_11165"
     CDS             2490..>4065
                     /locus_tag="PKZ49_11165"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HND37164.1"
                     /translation="MSKIFINYRRSDSAGYAGRLYDRLVKHFGADHLFMDIDQIEPGE
                     DFVEVLQKKLQAVQVAVVLIGKSWLDSKDETGQRRLDNPDDWVRIEIATLLERKIRII
                     PVLIGGAATPKSSQLPELLISLVRRQAFEISDTRFHSDVDKLIQALEKIIQLQLTQLP
                     PKPNLITPDSSIPDDVRHQKLTDAQHSPAQSRSESASVNKKPRSTISRFVMITIGCIA
                     LVFTVLSIVKLPITAQHDSSRDRISSPAPNSVVSESIQLNSEPVASASTEESVPAAGQ
                     QDSNRDGISTSPPERVATVATEQHAEPAVSTRSEESELASMYDFEPQMVRISPGSFIM
                     GSPESEAGRSADEGPQHPVKITYPFEIGRYEVTFAQYDAFAKDTQHQLPDDRGWGRGD
                     RPVINVTWHDAQAYVKWLSDKTGKKYRLPTEAEWEYVARAGTATAYWWGDRIGKANAA
                     CDGCGSQWDGKKTTLVGSFKPNAFGVYDTAGNVWEWTQDCWYGDYTRAPTDGSAWLEQ
                     NGGDCSRRVVRGGSWSS"
BASE COUNT         1162 a          847 c          981 g         1075 t
ORIGIN      
        1 gttctaattt atatctcgta aaagatatgt ttcgaagtga tttgaaaata agcgacccaa
       61 tactgaacca atcatgagca aaatccagca aacaaattct gaattgctcg aagaaataca
      121 gcgattacgg gctgaattag ccgccgtcaa gtggatgacg gaaaagcgag cgcaagagct
      181 ttatatgagc gaagaacgct tttcgctggc tatgcgaggc gccaatgatg gattatggga
      241 ttggaatcta gaaaccgatg agatttatta ctcaccgcgc tggaaaagca tgctgggtta
      301 tgaagcgcat caatttgcca ataatctaga tacgtgggct agtttggttc atcctgatga
      361 taaagccata gcattagcaa aaactcaaga ttatctagcg ggacgtacga cctcgcttga
      421 aattgaaatg cgtatgcgtc acaaagcagg ccatgatgta tttgtacttt ctcgcggttt
      481 tttagtgaga cgtgactcag atggtaaacc cattcgcctc gtcggtacgc atgttgatat
      541 tactgagcgt aaaaaatcag aagcgtttaa taaccaacat gctgaaattc ttgagatgat
      601 tgcggtagga cgtccagcat cagaaattta cgatgcgatt gcgctgatgt atgaatctcg
      661 ccatcccggc ttgcgttgct cgatgcttga attgagcggc aatattttac tgcatggcgg
      721 tgcacctagt ttgccaaaag cctattgcga agcagttcat ggcttgagaa atgggcccaa
      781 tgtcggttcc tgcggaactt cgacatttac cggaaaacgt gtgttagtcg aaaatattga
      841 aacagaccct aaatgggcag acattaagca ttttgcgtta ccccatggga tgcgttgttg
      901 ctggtctgaa cccatcaaga attccagtgg taaagtattg ggtgcatttg ggatgtacta
      961 taaccatccc gcgttaccca atgaacagga attaaaggat ttaatttctg cagcacgttt
     1021 ggccggtatt gttatggaac gagatcaagc gcaaaagcgt atccgtgcac ttgcttacac
     1081 tgatgaatta actggtttag caagtcgtgc ttccttcatt caacacatgg aagaaatcat
     1141 gaagacatca gaccgctata accggcggtt tggcttgtta tatatcgatc ttgataattt
     1201 caagtggatc aacgatagtt tagggcatga tgccggagat gctttactta aaactattgc
     1261 tacccggctg agaaatacgt gtcgggatac cgatttcatc gcgagattag gaggagatga
     1321 attttgtgtg ttggtcgagg aaattgaagc agattatgct gcaaatgtag caaaacgctg
     1381 ccttgcagcg atcgccgaac ccatcgtaat ttattcccgg aatctcactc caggttgtag
     1441 tatcggtatt gcgtattacc cagatgatgg aaataatcct gctgcattgc taaaagcagc
     1501 ggatacctcg ctctatactg caaaaagcaa tggtaagaat cagtacgcat tttattgcac
     1561 ggaattgacg cgcaaagcgc attaccgttt tcaaatcgaa caaaatctca aagaagcaat
     1621 agcaacgcag caactaacat tggtatatca gccgcaagtg aatgtaatta ccggtgagat
     1681 tttcggggtg gaagctttgt cgcgctggca tcatcctcaa ataggctcga ttccaccgag
     1741 tgattttatc gcaacggcgg aacgcattgg catgatcaaa tcgttaaccg aatgggtatt
     1801 aagaaccgca tgccagcagc tcatcgtttg gcaaactgaa ggattccacc gactacgtat
     1861 ggctgttaac atttccccca gcctttttct tgaaactgaa tttgtctcgt tggtgcaacg
     1921 cattgtccag gagactggaa tcaaaccaag ccaattgaaa ttagaagtta ctgaaggggt
     1981 agtacaaact gacccacgta atcttgcaat ttttcaagat ctgaaagcgc tgggtgtatt
     2041 acttgcgatc gatgattttg ggagtggtta ttcatctttt gcttcattga atcatcttaa
     2101 gacggattat ctgaaaatcg acaaatattt tatcgataag atgattaccg atcaaaaagc
     2161 gttcacgcta gtcaacacga tgatagaaat gggacataaa ctcggttatg gcatcattgc
     2221 agaaggggta gaaacccttg aacagcttag tatactccgt agtcttaatt gcgaaatcgt
     2281 gcaaggttat ctatttagtc aaccagtcag tgctgcccag ctttcagtgt tactacagag
     2341 tgataagcac aaaatgattc tcgaccaatg ccgggttaac aaaatcgctt aatttgttag
     2401 aaattactgg gtaatctgta aaattagcca agctagcaac aagaatctaa aatagcaggg
     2461 gtgtttgtta attttgctaa gcaccatcaa tgtcgaaaat tttcatcaat taccgtcgca
     2521 gtgacagcgc aggttatgca ggtcggctct acgatcgatt ggttaagcat ttcggggccg
     2581 atcatctgtt tatggatatc gatcagatcg aacccggaga agattttgtc gaagtcttac
     2641 agaaaaaact gcaagcggta caagttgccg tggtactgat tggaaaatcc tggcttgata
     2701 gtaaagatga aaccgggcaa cggcgcttag acaatccgga tgattgggtg cgaatcgaaa
     2761 ttgccacact gttggaacgg aaaattcgga ttattccagt attgatcggt ggcgctgcca
     2821 cgcctaaatc atcacaactg cctgaattat taatttcatt agtaagacga caggcctttg
     2881 aaatcagtga tactcgcttt cattcggatg tcgataagtt gattcaagcg ctcgaaaaaa
     2941 ttattcaatt acaactgact caattacccc ccaagccaaa tctgattact cctgactcat
     3001 caatacccga tgatgttaga catcaaaaac taaccgatgc gcaacattct ccggcccaga
     3061 gtcgctctga atctgctagt gttaataaaa aaccgcgatc taccattagt cggttcgtta
     3121 tgatcacgat tggctgtata gccttggtgt ttacagtatt gagtattgtt aaacttccta
     3181 taacagcgca gcatgatagt agccgtgatc gtatttcatc accagcacca aactcggttg
     3241 tgtctgagtc aattcagctg aattctgagc ctgtagcatc tgctagcacg gaagaatcgg
     3301 taccagcagc cggacagcaa gatagcaacc gtgatggcat ttcaacgtca ccaccagaac
     3361 gggttgcgac agtggcaacc gagcagcatg ctgagcccgc agtatcgact cgctctgaag
     3421 aatcggagct agcttcgatg tatgattttg aaccgcaaat ggtgcggatt tcacccggaa
     3481 gctttatcat gggttcaccg gaatctgaag cgggtcgaag tgccgatgaa ggaccgcagc
     3541 atccggttaa gattacctac ccattcgaaa tcggtcgcta tgaagtcacg tttgcacaat
     3601 acgatgcgtt tgccaaagat acacaacacc aattgcctga tgatcgtggg tggggacgcg
     3661 gtgatcgacc cgtgatcaat gttacctggc atgatgcgca agcctacgtt aaatggttgt
     3721 cagataaaac gggtaaaaaa tatcgtttac ccaccgaagc ggaatgggaa tatgtggcac
     3781 gcgcgggtac cgccaccgct tattggtggg gcgatcgcat tgggaaagct aatgcagctt
     3841 gtgatggttg cggtagtcaa tgggatggta agaaaacgac actggtaggc tcgtttaaac
     3901 ccaatgcgtt tggggtatac gatactgccg gtaatgtatg ggaatggacc caggattgct
     3961 ggtacggtga ttatacgcgt gcaccgaccg atgggtctgc ttggttggag cagaatggcg
     4021 gcgattgctc gcgccgggtg gttcgcggcg ggtcttggag cagct
//
LOCUS       DAOQKQ010000203         4064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_12968_length_4064_cov_5.838364, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000203 DAOQKQ010000000
VERSION     DAOQKQ010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12968_length_4064_cov_5.838364"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..731
                     /locus_tag="PKZ49_11170"
     CDS             207..731
                     /locus_tag="PKZ49_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase family protein"
                     /protein_id="HND37165.1"
                     /translation="MNIFRFGLLVMTALLAIISHASTQVPFGNEIKTVKNYNRATTQL
                     ATSGSLNEGSVQELAQHGFKTIIDLRTEAEGTASEKTAVESAGMTYINIPVTGAGVDA
                     EQLATFINAFEQAATPVLLHCASGNRAGAMWTTYRLHKGIAADIAFEEGRTAGMQPSL
                     EEKIKLTCQANKSC"
     gene            complement(742..1869)
                     /locus_tag="PKZ49_11175"
     CDS             complement(742..1869)
                     /locus_tag="PKZ49_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HND37166.1"
                     /translation="MLERHDTLECLNQTSPLREKLVKTHMVLNNHLPFIKRIAVAIYD
                     PKTSVLKTYLHSSGKEDPLSHYQTAIKDTPSLEALMKQGNPRIIKNKVTVERGRHEHT
                     QRIGREGYAASYTLPMFYNGVFFGFIFFNSSETDAFNETTLEQLDLYGHLISLMVINE
                     INSVQTLVAAISTASHMTHLRDPETGSHLDRMSRYARLIAKSLAEQYQLDDEYIERVF
                     MFSPLHDIGKLGIPDQILLKPGKLTEEEQTIMRTHATKGREMIDNMLANFGFDSIPDV
                     DILRNIAQFHHESINGQGYPAGLSGNSIPLEARIVAVADVFDALTSRRPYKEAWSNQE
                     AFAMLERMSGEKLDTDCVKALIDQQEEIEQIQEQFKEDPLG"
     gene            complement(2055..2798)
                     /locus_tag="PKZ49_11180"
     CDS             complement(2055..2798)
                     /locus_tag="PKZ49_11180"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HND37167.1"
                     /translation="MKNNRRNYYRILHVQPDAPLEIIRASYRAMMGPLKMHPDMGGNH
                     EVAALINQAWEVLSDPAKRAAYDRKIESNLPKTARKASSNRFTQTTQEKKHTNAPHQR
                     ADCSFNLSVCLFCTTPLPLEIGPETCCRSCGCPLAPPPMPAKMGKELFGRRRLPRVQR
                     SQSVIVHVTQEGGPLPAKLRNISDSGMSLELEAMVREGEVIRVLAHEFEALTQVLAVR
                     WYSQRWIASMRLVSIKFAKRQGVFISTYA"
     gene            3186..>4064
                     /locus_tag="PKZ49_11185"
     CDS             3186..>4064
                     /locus_tag="PKZ49_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HND37168.1"
                     /translation="MITLTSLLAITPLWLSEVHANIQDTIKIAGSGVYDKWSIEPTKP
                     SKSAPAIKPNSSQQEKWTIKPVESPAPAVAVAKPQETKPSTSYHRRSDSYATNPESDP
                     PRYVKRLSDIGVEAFKDITWLDVGLEHRTRYEWRNEDIRRIEGGEDNPIFLRNRAWIG
                     IKDILDPFRFAVEFQDSRVYNNKHAITDQERNEYDLINAYGELHFKKGLGVDDRGQDR
                     PIRFRVGRFAYESTDRRFIARNEWRNTTNSFEGFRLTLGREVNDWELDFFGMQPVMRL
                     QTKFDKANENLWFFGAV"
BASE COUNT         1178 a          872 c          832 g         1182 t
ORIGIN      
        1 ccatcgcgct gtagccttac cgctgcttca gcttgagaaa gcccatttgc cgatgtctca
       61 aggctagtta taatttcttc aagtgcttgg ctttgccata gaatgtttga gtggtgctta
      121 gaatcgatca cgctaccctc ttaattaact ggttaaatta tgctaagttt taaatgttgc
      181 ggtttaatct tttactggag tttttgatga acatctttcg atttggttta ttggtaatga
      241 cagcccttct cgcgattatt tcccatgcta gcacgcaagt tccgtttggt aacgaaatta
      301 aaactgttaa aaactataac cgcgctacaa cacaacttgc tacttctggt tcgttgaatg
      361 aaggaagtgt gcaagaactt gctcagcacg gattcaaaac aattatcgat ttgcgtactg
      421 aagctgaagg tacagcatcc gagaaaaccg ctgtagaatc agcaggcatg acttatatta
      481 atattccggt tactggcgca ggagttgacg ctgagcaatt agctacattt attaacgcct
      541 ttgagcaagc agctacaccg gttttattac attgtgcgtc gggtaaccga gcaggtgcga
      601 tgtggacaac ttatcgattg cataaaggaa ttgctgctga tattgccttt gaagaaggtc
      661 gcacggcagg gatgcagccg agtttagaag agaaaattaa attgacctgt caggcaaata
      721 aatcctgcta aaagccacat actagcctaa tggatcttct ttgaactgtt cttgaatttg
      781 ctcgatttct tcttgttgat cgatgagtgc tttaacgcag tctgtgtcaa gtttctcacc
      841 agacatgcgc tctagcattg caaatgcttc ttggttactc catgcttctt tatagggacg
      901 gcggctggtt aaagcatcga atacatctgc aacggccacg attctagcct caagcggtat
      961 tgaattgccg gatagtccgg caggatagcc ctgaccattg atactttcat gatgaaattg
     1021 tgcaatattt ctcaaaatat cgacatccgg aatactatca aagccaaaat tagccaacat
     1081 attgtcgatc atttctcgcc ctttggtggc atgtgtgcgc atgatcgtct gctcttcttc
     1141 cgtcagcttt cctggtttta gtaaaatttg gtcgggtatg cccagttttc cgatatcgtg
     1201 taacggtgaa aacataaata cccgctcgat atattcgtcg tcgagttgat attgttctgc
     1261 aagcgatttt gcaatgagtc gcgcatagcg tgacatgcga tccaaatggc taccggtttc
     1321 cggatcacgt aaatgagtca tatgcgacgc agttgaaatc gcggcaacca atgtttgaac
     1381 tgaattgatt tcattaatca ccatgagcga aatcaagtga ccatataaat ctaattgctc
     1441 taatgtagtc tcattaaatg catccgtttc gctggaatta aaaaaaataa acccaaagaa
     1501 taccccatta taaaacattg gtaatgtgta actcgccgca tacccttcac gtccgatacg
     1561 ctgagtatgt tcatgccgcc cacgttctac tgtaacttta ttcttaataa tacgaggatt
     1621 tccctgcttc attaatgctt ccagcgaggg cgtatctttg atggcggttt gataatgcga
     1681 taaagggtct tccttgccac tgctatgcag gtaagttttc aatacggaag tcttgggatc
     1741 ataaatagcc acagcaatgc gcttaatgaa tggtaaatgg ttatttagca ccatatgggt
     1801 tttaaccaat ttttcccgca gtgggctcgt ttgattcaag cactctaaag tatcatgtcg
     1861 ttccagcata atggataaat aatcaggcga ttaattagaa gtaaaagcag cgcggtcaac
     1921 atccacgtgc ttaccatgcc gacatgatag tcgaatttat tttgagtaat cgtacaaaaa
     1981 acgacttaaa aaataggtaa ttagcttatt caaactgcta atctatattg aaatgacaaa
     2041 gcgggctcat tgagctaggc ataagttgaa ataaaaacac cttggcgttt tgcaaactta
     2101 attgaaacta gtcgcatgct ggcaatccaa cgctgactat accaccgcac tgccaatact
     2161 tgtgtcaatg cttcaaattc atgtgccaga acacggatca cctccccttc acgtaccatg
     2221 gcctccagtt caagactcat cccactatct gaaatattac gtaatttagc cggtaacggc
     2281 cccccttctt gagtcacatg tacgatcacc gattgagatc gttgcacacg gggtaatcgc
     2341 cgccgcccaa acaattcctt acccatttta gcaggcatcg gtggcggcgc taacggacat
     2401 ccacaagaac ggcaacacgt ttcaggtcca atttctagtg gcaaaggtgt cgtacaaaac
     2461 aagcagactg atagattaaa tgaacaatca gcccgctgat ggggtgcatt ggtatgtttt
     2521 ttttcttgtg ttgtttgcgt gaaacgattg gaagaagctt ttcttgctgt cttaggtaaa
     2581 tttgactcga ttttccgatc ataagccgca cgtttggctg gatcgctcag cacttcccaa
     2641 gcttgattga tgagggcagc aacttcatga ttccccccca tatcaggatg cattttcagc
     2701 ggtcccatca tcgcgcgata gcttgcacga atgatttcca gcggtgcatc aggttgaaca
     2761 tgcaaaatac ggtagtaatt tcgacgatta tttttcattt ttttgatatc ggacaccatc
     2821 gacttggctt aaataaatcc tcctggttac tttagttact tccttatacg aaatagtgat
     2881 aatcaaatga attaatatta tttttaaata taaagaatga ttgataaagt gtgcatccat
     2941 tcgaaagcaa atagaacaaa tacacaacaa tcaaatttgc caattattgc ctaaaccaag
     3001 atcaagaaaa cacaatgaag ataataaaaa atagctttca ttacatttat gtactaatag
     3061 gagaattatt tattctccta ttaatcagta ttatcaaatt cagatttcta gtatttttgc
     3121 cattgattaa tggaatactt cagcgttatt ttatatttaa gcaattttta gcaaaatgca
     3181 aagtattgat aacactgaca agcttattgg caattacccc actctggctt agcgaagtcc
     3241 acgctaatat tcaagacaca attaaaatag cgggtagtgg cgtctatgat aaatggtcga
     3301 tcgaacctac aaaaccatct aaatccgcac ctgcaattaa gcctaatagt agtcaacaag
     3361 aaaaatggac aatcaaaccg gttgagtcac ctgctcctgc cgttgcagtg gctaaaccac
     3421 aggaaacgaa accatccaca agttatcacc gtcgttcgga cagctatgct accaacccgg
     3481 aatctgaccc accgcgctat gttaaacgtt taagtgatat cggcgtcgag gcattcaaag
     3541 atatcacttg gttagatgtc gggttagaac accgtacccg ctacgaatgg cgcaatgaag
     3601 atattcggcg cattgaaggg ggggaagata atccaatttt tctacgtaac cgcgcttgga
     3661 ttggtatcaa agatatatta gatccgttcc ggtttgcagt cgaatttcaa gattcacgcg
     3721 tctacaataa taaacacgcg attaccgacc aggaaagaaa tgagtacgat ctcatcaatg
     3781 cgtatggtga attgcatttc aaaaaggggc ttggtgttga tgatcgcggc caagaccgtc
     3841 ccattcgctt ccgagtcggt cgctttgcct atgaatcgac agaccggcgc tttatcgcac
     3901 gcaatgaatg gcgtaatacc acgaactcat ttgaaggctt tcgcctaacc cttggtcggg
     3961 aggttaatga ttgggagctt gacttctttg gtatgcaacc agttatgcgc ttgcagacta
     4021 aatttgacaa agccaacgaa aatctatggt tcttcggtgc agtc
//
LOCUS       DAOQKQ010000204         4047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_13069_length_4047_cov_6.491483, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000204 DAOQKQ010000000
VERSION     DAOQKQ010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13069_length_4047_cov_6.491483"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="PKZ49_11190"
     CDS             complement(<1..807)
                     /locus_tag="PKZ49_11190"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HND37169.1"
                     /translation="MSRLSKRRIKKIGLPAESLVYTGDRKGKNHINLTLIDYDAAHYE
                     ERLLQQVDACLPYKQKPTITWINVDGVHDPHLIEKLGEGFGIHRLVTEDLVSIVQRPK
                     CEDYGDYLFIVLKMLTYDAHENKIIPEQISLILGHQFLLSFQEDIQGDVFHLIRDHLR
                     TGKGKIRHRGTDYLAYCLLDALVDNYFIILEKIGDSIDALEEALMLQPGQEVMEQLYQ
                     LKRELLFLHKAVWPLREVISFLLRRESSLIQDSTIPYLRDVYDHVVQAIDS"
     gene            complement(903..1679)
                     /gene="rsmA"
                     /locus_tag="PKZ49_11195"
     CDS             complement(903..1679)
                     /gene="rsmA"
                     /locus_tag="PKZ49_11195"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HND37170.1"
                     /translation="MSHHPRKRFGQNFLIDNHLIAAIIHVINPISSDRLVEIGPGMGA
                     LTKPLVKILDHLDVVEIDRDIVQWLHAEFPARKLTIHAADALRFDFAQLGTGLRIIGN
                     LPYNISTPLLFHLSQYSTSVIDMHFMLQQEVVDRMVAEPSTSDYGRLSVMLQNQFEME
                     KLLAVPAESFMPVPKVQSAIVRMIPRATPIVPAAEHPLFTKLVTAAFAQRRKTLRNTL
                     KNYLVLADFAALKLDSSARAENLSVSDYAAVTRYLNTDTA"
     gene            complement(1691..2680)
                     /gene="pdxA"
                     /locus_tag="PKZ49_11200"
     CDS             complement(1691..2680)
                     /gene="pdxA"
                     /locus_tag="PKZ49_11200"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HND37171.1"
                     /translation="MLTLALTAGEPAGIGPDLCIQWAQQHSLPYRVVVIADREMLEQR
                     AHRLGCSLTFVPYIASEWKPQQAGTLEIYPVPVQHPVDPGVLDPANADYVLETLKIAV
                     SGCQLQQFHAMVTAPVHKGVINGAGIPFTGHTEFLADLTQSQVVMMLVGGGMRVALAT
                     THLPLNAVADAITASTLEQRLRIIDRDLRTRFWIEHPCIAVAGLNPHAGESGHLGREE
                     IEIIIPLLTKLRAEGMNLIGPLPADTLFNPVKLQGYDCVLAMYHDQGLPVLKYASFGS
                     GVNVTLGLPLIRTSVDHGTALDLAGTGQADIGSFHQAIMMASTLAEKAARFQQ"
     gene            complement(2690..4042)
                     /locus_tag="PKZ49_11205"
     CDS             complement(2690..4042)
                     /locus_tag="PKZ49_11205"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HND37172.1"
                     /translation="MKKLFFVLISLTISIVFTSFLMSHDIAAQEEPVLTSLPVTRSVQ
                     SIDHIVAIVNEEVITRNELDEVIRTTVKQLQKQGVQLPPFDIIEKQLLERTIMNRAQL
                     QRAKEVGIAVTDTELDQTIRKIAKENNLSMDEFYYALQQDGMTLPEFREEIQNELLIS
                     RLKEREVNNRVTVTEGEIDNFLRTQETSAIGNDEYRVAHILVQILEQMDSAQIEARQQ
                     KAENALKRLQEGVDFAQISAELSDAPNALQGGEIDWRPITQMGPMFSDILIKMQPGDL
                     TPVIRSPIGFHILKLLGRRPQETPVVMVNQTHARHILIKINELTSEDDAHRVIAQIRE
                     RIAKGADFAETAKGMSEDASANNGGDLGWLSPGDTVPEFERAMEALMPGQMSQPVQTQ
                     FGWHLIQVMERRTQDVSLDRHRHSARQAIRNRKADMIMQEWLQQLRDQTYVEYRTEDS
                     "
BASE COUNT         1132 a          958 c          836 g         1121 t
ORIGIN      
        1 actatcgatc gcttgtacca catgatcgta aacatcccgt aaataaggta tggttgaatc
       61 ttgaatcaac gacgactctc gcctcaataa gaacgaaata acttcgcgga gtggccatac
      121 ggctttatgg aggaatagca attcacgttt caattgatat agctgttcca tgacttcctg
      181 accgggttgt agcattaagg cttcttcaag cgcatcgatg gaatcaccga ttttctccag
      241 aataataaaa taattatcaa ccagcgcatc caataagcaa taagccaaat agtccgttcc
      301 gcgatgtcga attttgcctt ttcccgtgcg cagatgatcg cgaatcaaat gaaatacatc
      361 gccctgaata tcttcttgga aagaaagtaa aaattgatgc cccaaaatca aactgatttg
      421 ctcaggaata attttgtttt cgtgcgcatc ataggtcaac atcttcagaa cgataaataa
      481 ataatcaccg taatcttcac atttaggacg ctgcacaata ctaaccagat cttctgtcac
      541 cagacgatgt attccaaatc cctcccctaa cttttcgatt aaatggggat catgaacacc
      601 atcgacatta atccaagtaa tcgtgggttt ttgcttataa ggtagacaag catcaacctg
      661 ttgcagcaaa cgctcctcat aatgagctgc atcatagtcg atcaacgtca aattgatatg
      721 gttcttccct tttctatcac cggtgtagac caagctttca gcgggaaggc caattttctt
      781 aattctgcgt ttcgataaac gtgacatcgt gctttgtcct ggtaaaatct gaatgatccc
      841 agctattcta gcgtagctgc tgcgtcacaa tttgatttta tacagccaac ttacgctatg
      901 gatcacgctg tatcggtgtt taaatagcgc gtcactgcag cgtaatccga taccgataaa
      961 ttttctgctc ttgcacttga atctagtttt aacgctgcaa aatcagccaa tacaagatag
     1021 tttttcaagg tattgcgcag cgttttgcgg cgttgagcaa aagcagcagt aactaatttc
     1081 gtgaataagg gatgttctgc tgctggcacg attggtgtag cacgaggaat catacgaaca
     1141 atggcagatt gaactttggg aacaggcata aacgattctg ccggaacagc aagcaatttc
     1201 tccatttcaa attggttttg cagcatcacg gaaagccgcc catagtctga agtagatggt
     1261 tcggcaacca tacggtcaac gacttcttgc tgcagcataa aatgcatgtc gatcaccgat
     1321 gtactgtatt ggctcaggtg gaaaagtagc ggtgtcgaga tgttataagg caaattaccg
     1381 ataatgcgta aacccgttcc caattgcgca aaatcaaagc gcaaagcatc ggcagcatga
     1441 atggtcaatt ttcgcgctgg aaattcagca tgaagccatt gtacgatgtc tcgatcgatt
     1501 tcaacgacat caagatgatc gagtattttt actaaaggtt tggtcaatgc ccccatacca
     1561 ggcccaattt ctaccaatcg gtcactactt attgggttga tgacatgaat aatcgctgca
     1621 atgaggtgat tatcgatcaa aaaattttga ccaaatcgct tacgaggatg atgggacaca
     1681 gaaatttagg ttattgttgg aaacgagctg ctttctcagc aagcgtggat gccatcatga
     1741 tcgcttgatg gaaactaccg atatcagcct gacccgttcc agctaaatca agtgcagtcc
     1801 catgatcaac tgatgttcga atcagtggaa gccccaatgt gacattcaca ccactcccaa
     1861 aactggcata tttaagcacg ggcaaacctt gatcatgata catcgccaac acacaatcat
     1921 agccttgcaa ttttacggga ttaaataagg tatctgctgg cagaggaccg atcagattca
     1981 ttccttcagc gcgcaacttt gttaatagtg gaatgatgat ttcaatttct tcacggccaa
     2041 gatggcctga ttctcccgca tgcggattca gtcctgcgac agcaatacaa ggatgctcaa
     2101 tccagaaacg ggttctaaga tcacgatcaa taatccgtaa tcgctgttct agcgtgctag
     2161 ccgtgattgc atcggctact gcgttcagcg gcaaatgcgt cgtagccagc gcaactcgca
     2221 tccctccccc gaccagcatc ataaccactt gactttgtgt taggtcagct agaaattcag
     2281 tatgccccgt gaagggaatt ccagctccat taatcacgcc tttatgaact ggtgcggtga
     2341 ccatggcatg gaattgctgc aactgacaac cgctaaccgc aattttaagg gtttccaaca
     2401 cataatctgc attcgcaggg tctagcacac caggatcaac cggatgctgt actggcaccg
     2461 ggtaaatttc taaagtcccg gcttgctgag gtttccactc tgaagcaata taaggtacaa
     2521 aagttaatga acaccccaat cggtgcgcgc gctgttccaa catttcacgg tctgcaatca
     2581 cgaccactcg atagggaagc gaatgctgct gagcccactg tatacataaa tcagggccaa
     2641 taccggcagg ttctcctgct gtcagcgcta acgttaacat tgactgcaat taactatctt
     2701 ctgtgcgata ctcaacatag gtttgatctc gtaattgctg taaccattct tgcatgatca
     2761 tatctgcctt acgattacga atggcttgac gggcagaatg acgatgacga tctaaactca
     2821 catcctgggt gcggcgctcc atcacctgga ttaagtgcca accaaactga gtttgaaccg
     2881 gttgactcat ctggccaggc atcaatgcct ccatcgccct ctcaaattca ggaacagtat
     2941 cgcccggcga tagccaacct aaatcaccac cattgttagc acttgcatct tccgacatgc
     3001 ctttagcggt ttcagcaaaa tcagcgcctt ttgcaatgcg ttctctaatt tgtgcaatta
     3061 cccgatgcgc atcatcttct gaagtcaatt cattgatttt aattaaaata tgtcgtgcat
     3121 gcgtttgatt gaccatcaca actggtgttt cttgaggcct acgcccgaga agtttcaaaa
     3181 tatgaaaccc gattgggcta cggatgaccg gtgtcagatc cccaggttgc attttgatca
     3241 aaatatcaga aaacatcggc cccatctggg tgataggtcg ccaatcgatt tctccacctt
     3301 gcaaggcatt cggcgcatca gataactctg ccgatatttg ggcaaaatcc actccctcct
     3361 gtaagcgctt caatgcattc tctgctttct gttgccgtgc ttcgatttgc gccgagtcca
     3421 tttgttccaa tatctgaact aggatatgtg cgacacgata ctcatcgttg ccaatcgcag
     3481 aagtttcttg tgtccgtaaa aaattatcga tttccccttc ggtcacagtg actcgattat
     3541 tcacttcgcg ttctttgaga cgagaaatca gcaattcgtt ttgaatttct tcacgaaact
     3601 cggggagtgt catgccatct tgttgcaaag cataataaaa ctcatccatg gatagattat
     3661 tttctttagc aatcttacga atcgtctggt cgagctcggt atcggtgaca gcaatcccga
     3721 cttctttggc gcgctgcaat tgcgctcgat tcatgatggt gcgctctaat aactgttttt
     3781 cgataatatc aaacggaggc aactgaacac cctgcttctg aagctgtttg actgtcgtac
     3841 ggatgacttc atcaagctca ttgcgcgtaa tgacttcttc attgacaatg gcaacgatat
     3901 gatcaatcga ttgtaccgat cgtgtaaccg gcaagctggt taatactggt tcttcttgtg
     3961 cagcaatgtc atggctcatc aaaaaagaag taaaaacaat actgatagtt aagctgataa
     4021 gtacaaaaaa tagttttttc attaaat
//
LOCUS       DAOQKQ010000205         4029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_13179_length_4029_cov_4.905637, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000205 DAOQKQ010000000
VERSION     DAOQKQ010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13179_length_4029_cov_4.905637"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..1223
                     /gene="nirK"
                     /locus_tag="PKZ49_11210"
     CDS             120..1223
                     /gene="nirK"
                     /locus_tag="PKZ49_11210"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HND37173.1"
                     /translation="MNNKAVQAVIGLGLLCFTASVAFATKHDISKLPRVKQTLVAPPF
                     VPEHDQVSKGPKVVEVTMETIEKQIEIAPGVKYWALTFNGTVPGPLIVVHEGDYVELT
                     LKNPKTSTMAHNVDFHAATGALGGAQLTLVQPGEEVVLRWKALKNGVFVYHCAPGGTM
                     IPFHVISGMSGAIMVLPKDGLKDNKGKSVKYDRAYYIGEQDFYVPKGADGKYKSYDQP
                     AAGMADILGVAAGLIPSHVVFNGAVGAITGDNALKAKVGEKVLFIHSQANRDSRPHLI
                     GGHGDLVWQGGSFNDPPITNQETWFVPGGAAVAALYDFKMPGLYVYLSHNLIEAVLLG
                     AAAHMNVEGEWDNDIMEQLKKPASFNKDAAMDH"
     gene            1360..2646
                     /gene="gshA"
                     /locus_tag="PKZ49_11215"
     CDS             1360..2646
                     /gene="gshA"
                     /locus_tag="PKZ49_11215"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HND37174.1"
                     /translation="MAVPHLTTALSGPILALEKRILEAMPAIEHWLRGKWQQHTIPFY
                     CSVDLRNSGFKLAPVDTNLFPGGFNNLNTDFLPLCVQAMMAAVEKICPEAHSILIIPE
                     SHTRNIYYLQNVHVLERIMRHAGLNIRIGSLIPEITEPTPINLPDGNTLLLEPIVRKG
                     NRLSVGNFDPCAILLNNDLSSGIPDNLKNLDQIIIPPIHAGWTTRLKSQHFTAYDRVA
                     QEFAELIGIDTWLINPYFNSCGKIDFYEEKGVDCVAATVDEILSNIKEKYTQYGVKEE
                     PFVIVKADAGTYGMGIMTVKDGADVYKLNRKQRNKMAKIKEGLSVSHVLVQEGVYTFE
                     SIHQAVAEPVIYMIDRYVVGGFYRVHTGKGIDENLNAPGMHFVPLAFDTSCVLPNHAA
                     LPDSVPNRFYAYGVVARLALLAAAVELEQHAPKVGA"
     gene            2675..3622
                     /gene="gshB"
                     /locus_tag="PKZ49_11220"
     CDS             2675..3622
                     /gene="gshB"
                     /locus_tag="PKZ49_11220"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HND37175.1"
                     /translation="MNLVFIIDPLESLKIEKDSSIAMMQEAIVRDHRVFVMHQGDLSY
                     ENNIVMARVNELQPKQSGEIRAHWFDLGPKEVVPLAQFDIVLMRKDPPFDMEYIYSTY
                     LLEIAQQQGVLVINNPRSVRDHNEKLAITKFPDFIPATLVTRNEHLIREFLSQHQDIV
                     LKPLDGMGGASIFRVHSTDPNLSVILEMMTHYGTRTIMVQRFLPEIVLGDKRILLIAG
                     KPVPYALARKPKSGESRGNLAAGGIGIVQPLSERDLQIATTIGPVLNTLGLILVGLDV
                     IGDSLTEINVTSPTGMREITKQTGFNVAGMMIEALEALS"
     gene            complement(3639..>4029)
                     /locus_tag="PKZ49_11225"
     CDS             complement(3639..>4029)
                     /locus_tag="PKZ49_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hopanoid biosynthesis-associated RND transporter
                     HpnN"
                     /protein_id="HND37176.1"
                     /translation="LVVITLLPLLLAALFTVATTALLNIPFNFANVIALPLLLGIGID
                     SSIHMVYRSLSSVENQRSLLQTSTGSAIFYSALTTMAGFGSLILSTHQGTASMGMLLA
                     VGILYILICVFIILPTLLTRFKHKLNF"
BASE COUNT         1181 a          729 c          929 g         1190 t
ORIGIN      
        1 ttttgtttgt gatagttgga gtttatgcgg ttgcggtaat gtgggaagca gtaccgaaaa
       61 aagaatggcg cttctgatta gataacattg cgcaattttt aaaatttatg gagggatata
      121 tgaataataa agctgtacaa gcagtgattg ggctagggct gctctgtttt actgcttctg
      181 ttgcattcgc aacaaagcat gatatttcaa aattgccacg tgtaaaacaa acacttgttg
      241 caccaccgtt tgttcctgag catgatcaag tttcaaaagg ccctaaagtg gttgaagtaa
      301 cgatggaaac cattgaaaag caaattgaaa ttgctcctgg tgttaaatac tgggcattga
      361 cttttaatgg tacggttcct ggtccgctta tcgttgttca tgaaggggat tatgtagagc
      421 ttacattaaa aaaccctaaa acgagcacaa tggcacataa cgttgatttc catgcagcaa
      481 caggtgcatt aggcggtgct caacttacat tggttcaacc tggtgaagaa gtggtattgc
      541 gctggaaagc actcaaaaat ggcgtctttg tctatcattg cgcgccaggt ggaacgatga
      601 ttccattcca cgttatctct ggtatgagtg gtgcgattat ggtattacca aaagatggtc
      661 tgaaagacaa taaaggtaaa tcagttaaat acgatcgtgc atattatatc ggtgagcaag
      721 atttctatgt acctaaaggt gcggatggta aatacaaatc ctatgatcaa cctgctgcag
      781 gtatggctga tatcttagga gtagctgcag gccttatacc aagtcacgta gtgtttaacg
      841 gtgctgttgg agctataacc ggtgacaatg cattgaaagc taaagtgggc gaaaaagtat
      901 tatttatcca ctcacaagct aaccgtgatt cacgtccaca tctgattggt ggtcatggtg
      961 acttagtatg gcaaggtggt tcatttaatg atcctccaat tactaaccaa gaaacatggt
     1021 ttgtacctgg tggagcagct gttgcagcac tttatgattt caaaatgcct ggtctctatg
     1081 tatacctgag ccacaactta attgaagctg tattgcttgg cgcagcagct catatgaatg
     1141 ttgaaggtga atgggataat gacatcatgg aacaactcaa aaaacctgca agctttaaca
     1201 aagatgcagc gatggatcat taattcataa agaagcagtt tagctttttt agagaaaacg
     1261 accccttttt aggggtcgtt ttttttgtaa tgatggcatt agcgggtaaa gccaattagc
     1321 atgatataat ctctaattca atcattctga tttgctttga tggcagtacc acatcttact
     1381 acggccttaa gtggaccaat tctcgcgctt gaaaagcgta ttcttgaagc aatgcccgca
     1441 attgagcact ggttacgagg aaaatggcag caacatacta ttccttttta ctgttcagtt
     1501 gatttaagaa atagtgggtt taaactagcg ccggtcgaca ccaatctgtt cccgggtggt
     1561 tttaataatc tcaacaccga ctttttacca ctttgtgtac aagcgatgat ggcggcagta
     1621 gagaaaattt gccctgaagc gcatagtatt ctcatcattc cagagagtca cacacgaaat
     1681 atttattatc ttcagaatgt gcatgtatta gagcggatta tgcgacatgc aggattaaat
     1741 atccgaattg gttcgttgat tccggaaatt acagaaccta cacctattaa tctacctgat
     1801 ggaaatacgc tacttctaga gccaatcgta aggaaaggaa atcgtctatc ggttggaaac
     1861 tttgatccct gcgctatttt gttaaataat gatctttcaa gtggaattcc tgataactta
     1921 aaaaatttag atcaaattat tattcctcca attcatgcag gatggactac gcgcttaaag
     1981 tctcagcatt ttacggctta tgatcgggta gcacaggagt ttgcggaact gattggtatc
     2041 gatacatggc ttattaatcc ctattttaat tcttgtggaa aaatcgactt ttatgaggag
     2101 aaaggggtgg attgtgtcgc agcaaccgta gatgaaatac tcagtaatat taaagaaaaa
     2161 tatactcaat atggtgtcaa ggaagagccc tttgtaattg tcaaagcgga tgcaggtacc
     2221 tatggcatgg ggatcatgac ggtaaaagat ggggctgatg tctataagct taatcgcaag
     2281 cagcgcaata agatggctaa aatcaaagaa gggttgtctg tgagccacgt tttggtacag
     2341 gagggtgttt atacttttga gagcatccat caagccgttg ccgagcccgt gatctatatg
     2401 atcgatcgtt atgttgtagg aggattctat cgtgttcata ccggtaaagg tatcgatgag
     2461 aatttgaacg cacctggtat gcattttgtt ccattagcat ttgatacttc ctgtgtactt
     2521 cccaatcatg cagcgcttcc tgattcagtt cccaatcggt tctatgctta tggtgttgta
     2581 gcgaggctgg ctttacttgc cgctgccgtt gaactagagc agcatgcacc taaggttggc
     2641 gcttaggtcg attgcaagtt atggctgatt tcgtatgaat ttggttttta ttattgatcc
     2701 gttagaatcg ctcaaaattg aaaaagactc tagtattgcg atgatgcaag aagccatcgt
     2761 gcgtgatcat cgggtttttg taatgcatca aggtgacctg agttatgaaa ataatatcgt
     2821 catggccaga gtaaacgagt tgcagcccaa gcaatcggga gagattcggg cacattggtt
     2881 tgatctaggc cccaaagaag tcgttccatt agcacaattc gatatagtct tgatgcgtaa
     2941 agatccccct ttcgatatgg agtatattta tagcacctat ttgctagaaa tcgcacagca
     3001 acagggtgtg ttggtcatta ataaccctag gagtgttaga gatcataatg agaaactggc
     3061 aattacaaaa tttcccgatt ttattcctgc cacgttagta actagaaatg agcatttgat
     3121 tcgggaattt ctgagtcaac atcaagatat cgtattgaaa ccgttagatg ggatgggagg
     3181 cgctagtatt tttcgtgtgc atagtactga tcctaacttg agcgtaattc tggaaatgat
     3241 gactcattat gggacgcgta ccattatggt ccagcgtttt ctcccagaga ttgtattggg
     3301 tgacaaacga attttgctta ttgccgggaa accggttccc tatgcacttg cacgtaagcc
     3361 aaaatcagga gaaagccggg gaaatctggc tgcgggtgga attggtattg ttcagccttt
     3421 gtcagagcgt gatttacaaa ttgcaactac gataggcccg gtcctaaata cgctaggctt
     3481 aatattggtt gggcttgatg tgatcgggga ttcattaact gaaatcaatg tgacgagtcc
     3541 gactgggatg cgagaaataa ccaagcaaac tggatttaat gttgctggaa tgatgataga
     3601 ggcccttgaa gcccttagct gattatttgt cgcagtattt agaaattaag cttatgttta
     3661 aaacgagtaa gcagggtagg tagaatgata aagacgcaaa tcaagatgta aagaattcca
     3721 acggctaaca gcatacccat gcttgctgtt ccctgatgag tagatagaat caaactacca
     3781 aatcccgcca tagtcgtcag tgcgctataa aatatggcag agcctgtact cgtttgtagc
     3841 aagcttcttt ggttttctac agaactcaag cttcgataaa ccatatggat gctactatcg
     3901 ataccaattc ctagtaaaag tggtaatgca attacattag cgaaattaaa tggaatgttt
     3961 agcagcgctg ttgttgcaac tgtaaataat gctgctagaa gtaatggcag aagcgttatt
     4021 acaacaagt
//
LOCUS       DAOQKQ010000206         4000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_13344_length_4000_cov_5.185044, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000206 DAOQKQ010000000
VERSION     DAOQKQ010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13344_length_4000_cov_5.185044"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /gene="hisG"
                     /locus_tag="PKZ49_11230"
     CDS             <1..254
                     /gene="hisG"
                     /locus_tag="PKZ49_11230"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380175.1"
                     /note="short form of enzyme; requires HisZ for function;
                     catalyzes the formation of N'-5'-phosphoribosyl-ATP from
                     phosphoribosyl pyrophosphate; crucial role in histidine
                     biosynthesis; forms heteromultimer of HisG and HisZ;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HND37177.1"
                     /translation="EKGMHVDLIKLYGSMELAPLVGLADAIVDLVSSGNTLKANHLKA
                     VEEIRPISARLIINQAALKLKRATIQPMLESFESVIGVA"
     gene            276..1574
                     /gene="hisD"
                     /locus_tag="PKZ49_11235"
     CDS             276..1574
                     /gene="hisD"
                     /locus_tag="PKZ49_11235"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HND37178.1"
                     /translation="MINIKRLSSLDSHFNEVLDALLAFETTQDNAIIETVARILSEIR
                     IHGDSALLDYTRRFDHLHANTLAELELPMQSCQQALQNLSPAESEALKHAAQRIKQYH
                     EKQLSQSWQYTEADGTQLGQKITALDRVGLYVPGGKASYPSSVLMNAIPAKVAGVDEL
                     IMVVPTPRGEKNQLVLAAAAIAGVDRVFTIGGAQAIAALAYGTQTVPKVDKIVGPGNA
                     YVATAKRLVFGQVGIDMVAGPSEILVICDGHVNPDWIAIDLFSQAEHDEQAQSILLSP
                     DQSFIDAVQASIDRLIVDQARKAVIQTSLENRGALIKVRDLTEACKIANRIAPEHLEL
                     AVAEPEQWVDQIKHAGAIFMGYHTCEALGDYCAGPNHVLPTSGTARFSSPLGVYDFQK
                     RTSLISVSPQSAHQLGQIASTLARGEGLQAHALSAEYRCN"
     gene            1607..2191
                     /gene="greB"
                     /locus_tag="PKZ49_11240"
     CDS             1607..2191
                     /gene="greB"
                     /locus_tag="PKZ49_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HND37179.1"
                     /translation="MNKAFVKENDDSEELEAEAVSTPLPVGSKNYITPAGHARLMEEY
                     QWLLNKERPDVTAIVSWAAGNGDRSENGDYIYGKRRLREIDKRLRFLSKRLEIAEIID
                     PTLPREDSMRIFFGATVCYLDQTGVERTVSIVGIDEIDPFKGYISWISPVARALISAQ
                     EGDTVYLRTPTSVEKLEILSVIYQSIAIPAFQPI"
     gene            2446..3270
                     /locus_tag="PKZ49_11245"
     CDS             2446..3270
                     /locus_tag="PKZ49_11245"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HND37180.1"
                     /translation="MESEYANRTNRENQLIGDQQGFFTPGFCYVCSKNVDFFSNFAHA
                     FLNADARKIPNWREHMVCPHCHLNNRMRGAIQVFEQLCEAKLDDNIYLTEQKTPLYQW
                     FQQNYLKVIGSEYLANTVPYGSLDKDRIRNETLTGLTFETNTFDYILSFDVFEHIPEY
                     QVAFQETLRCLKPRGKLIFTVPFNRGSDNSIVRARLASDGTIEHILPPEYHGDPLNSK
                     GVLCFYHFGWELLDQLCEIGFSSATAYLYWSEELGYLGFEQILFVAQKSDAEKMLS"
     gene            3771..3971
                     /locus_tag="PKZ49_11250"
     CDS             3771..3971
                     /locus_tag="PKZ49_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37181.1"
                     /translation="MEKVNHNDSPASAWQMKNWLSFIAVVFFILASPIANSMDVSVKP
                     ISDKLKLQASETRWIRSCLVKY"
BASE COUNT         1129 a          844 c          878 g         1149 t
ORIGIN      
        1 ccgagaaagg catgcatgtc gatctaatta aattatatgg atcgatggaa ttagcgcctc
       61 tcgttgggct ggcagatgct atcgtcgatt tggtttccag cggtaatacc ttgaaagcaa
      121 atcacctcaa agcagttgaa gaaattcggc caatttctgc gcggctaatc atcaatcaag
      181 ctgcgctgaa gctgaaacgt gcaaccattc aaccgatgct cgaatccttt gaaagtgtta
      241 tcggtgtcgc ttaatcgctt caggaagaac agtctatgat caacattaaa cgtttatcct
      301 cgcttgattc tcattttaat gaagtattag atgcgttgct ggcgtttgaa accacgcaag
      361 ataatgcgat cattgaaacc gtggcgcgta ttctgtccga aattcgtatc catggggata
      421 gtgcattgct cgactataca cgccgctttg atcatttgca tgctaatacc cttgcagaac
      481 tcgagttgcc catgcaatct tgccagcaag cgttgcaaaa cctgagccct gcagaaagcg
      541 aagcccttaa acatgcggct cagcgcatta agcaatatca cgaaaaacaa ctatcgcaat
      601 cttggcaata taccgaagca gatggtactc agctcggaca aaaaatcact gcgctggatc
      661 gagtcgggtt gtatgtgcct ggtggcaaag cttcttatcc ttcgtcggta ctgatgaatg
      721 ccattccagc caaagttgcc ggtgtcgatg agctaatcat ggttgtcccc actccgcgcg
      781 gcgaaaaaaa tcagcttgtc cttgcagcag ctgcaattgc cggcgtagat cgagtattca
      841 cgattggcgg tgcacaggcc attgcggcat tagcttacgg cactcaaacg gttcccaaag
      901 tcgataaaat cgtgggccca ggcaatgctt atgtcgccac cgcgaaacgc ttggtttttg
      961 ggcaagtcgg tatcgatatg gtcgctggcc cttcggaaat tttggtcatt tgcgatggcc
     1021 acgttaatcc agattggatt gccatagatt tattttctca agcagaacat gatgaacaag
     1081 cacaatcgat tctgctttct ccggatcaat catttatcga tgccgttcaa gccagtatcg
     1141 atcggttgat tgtcgatcaa gcccgtaaag ctgtgataca gacatcactc gaaaatcgcg
     1201 gcgctttgat caaagttcgt gatctgacgg aagcttgcaa gattgccaac cgtatcgcac
     1261 cggagcatct tgaattagcg gttgctgagc ctgaacaatg ggtcgatcag atcaaacacg
     1321 ctggagctat tttcatggga taccatacct gtgaagcgct tggggattat tgtgcaggtc
     1381 ccaatcatgt attgccgaca tcgggtaccg cacggttctc ttctccatta ggcgtgtatg
     1441 attttcagaa acgcacgagc ttgatcagcg tttcaccaca gagcgcccat cagttaggcc
     1501 aaattgcttc taccttagcg cgtggtgagg gtttgcaagc gcatgcctta tcagccgagt
     1561 atcgctgtaa ctgacatcaa acattgcagt aatcgacagt actagcatga ataaagcgtt
     1621 tgtcaaagaa aacgacgatt cggaagaact cgaagcagaa gctgttagta cccctttacc
     1681 agtaggcagt aaaaactaca tcacgccggc aggtcacgcc cgcctcatgg aagaatacca
     1741 atggttgctc aacaaagagc gcccggatgt aactgcgatc gtatcgtggg cagccggcaa
     1801 tggcgatcgt tctgaaaatg gcgattatat ttatggcaag cgacgcttac gtgaaatcga
     1861 caaacgctta cgctttttgt ccaagcgact tgaaatcgct gaaattatcg acccgacgct
     1921 gccgcgtgaa gacagcatgc gaattttctt tggtgcaacc gtttgctact tagatcaaac
     1981 gggtgtggag agaacggtat ctatcgtcgg tatcgatgaa atcgatccct ttaaaggtta
     2041 tattagctgg atctctccgg ttgcacgggc gctgatctct gcacaagaag gggataccgt
     2101 ttatttgcgc acaccaacga gtgtcgaaaa gctggaaatc ttgtcggtta tttaccaatc
     2161 gattgcaata ccggcgttcc aaccgattta agttaaattt ctttttgatt caagtgccta
     2221 attggtaaat aaactcgttt ttgtgcaatt taaaaaatga tgcacctgct cattaaaaag
     2281 atccgtaatc gtgctcaatt tttttggaat cgaatatttt gtactaaaac tagtaaaaat
     2341 gatttgttga cgcagaaaca ttgtcgacag cgcagccaat ttgatcgtcc attaaattta
     2401 gagtctattt cctcattcgc agcatatgtg acatatcacg aaaagatgga atcagaatat
     2461 gccaatcgaa caaatagaga aaatcaatta ataggcgatc agcaagggtt ttttactcct
     2521 ggtttttgct acgtttgtag caagaatgtg gattttttca gcaactttgc gcatgctttc
     2581 ttgaatgctg atgctcgtaa aattccaaat tggcgggaac acatggtatg tccacattgc
     2641 catttgaaca accggatgcg cggagcgatt caagtctttg aacaactatg tgaagccaaa
     2701 ctcgatgata atatctattt gaccgagcaa aaaactcccc tctatcaatg gttccagcaa
     2761 aattatttaa aggttattgg tagtgagtat ctagccaata cggttcctta tggaagcctt
     2821 gataaagatc gtattcgaaa tgagacgctt acaggtttga cctttgaaac taacacgttt
     2881 gactatattt tatcctttga tgtattcgag catattcctg aataccaggt tgcttttcaa
     2941 gaaaccttac gttgcttaaa acccagagga aagctaatct ttaccgtacc attcaaccgt
     3001 ggttcggata acagcatagt acgagcacgg ttagcttcag atggaacaat agaacatatc
     3061 ttgccaccgg aataccatgg cgatccactc aatagcaaag gtgttttgtg tttttatcat
     3121 tttggatggg aactactcga tcaactatgt gaaataggct tttcatctgc aacagcctac
     3181 ttatattggt cggaggaact aggttacctg gggtttgagc aaatactttt tgttgctcag
     3241 aaaagtgatg ctgagaaaat gctgagttag tgattttttt cgcgttttga ttatactgtc
     3301 attgattcgc tcatcgcatc acctccttaa tagtcgaaac agtactcacc agcagtttct
     3361 ttctgctcag tgattttatc gatttgtgac cagtattcaa tctgtttgtt tcggtactga
     3421 acgatgttga acttgtgcat aatgcctagc tttggcgact gggttctcag acaactttat
     3481 atttacaagc ataatcaccc ctgagcaaaa ttccattcta gtttgattat tcaaggaaag
     3541 acaaggttgc tgataggttg tatatgaatt ttattggtga agattagcgc tattgctgag
     3601 gtatcctaag ttgaagctta ttgaacacca tgtgaatcat actcatcagg ctaaaaatat
     3661 tacgaatcca tagaatttga tcttttatat agaatcaggg ctgtttccac gatcttagta
     3721 aatttgataa agcatccaga tagaacatta acagggagat aatcgatctt atggaaaagg
     3781 ttaatcataa tgattcaccg gctagcgctt ggcagatgaa aaattggcta tccttcatcg
     3841 cagtagtttt ttttattctg gcatcaccca ttgctaattc gatggatgta tcggtaaaac
     3901 cgatctctga caaactaaaa ttgcaagcaa gtgaaacgag atggataaga agctgtctgg
     3961 taaaatatta gacgcatttt gcaagtgtta ttttcatcat
//
LOCUS       DAOQKQ010000207         3872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_14074_length_3872_cov_5.644223, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000207 DAOQKQ010000000
VERSION     DAOQKQ010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14074_length_3872_cov_5.644223"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="PKZ49_11255"
     CDS             <1..276
                     /locus_tag="PKZ49_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HND37182.1"
                     /translation="TLGRRLVDGAKIVRIFGQEIVVRSDLYTIGGLSAHADQAGILNW
                     LKAFKTPPNHTYIVHGEPSNANALANIIKQQLNWPVSIPERFATVTL"
     gene            complement(297..1538)
                     /locus_tag="PKZ49_11260"
     CDS             complement(297..1538)
                     /locus_tag="PKZ49_11260"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HND37183.1"
                     /translation="MKKTFLLDRLGWLIIVSLVLLSISHSKADAVTDWNKWAGNIVVD
                     AEIGPLPAERALAIVQAAVYESVNAITRRYSTSDIELLPPNPDASIEAAIAEANRVTL
                     MQLAPNQQASIEKTYQTAISAISEGSAKRAGIELGAKVAQAILAKRTQDGAADKESYR
                     PNTSAGVYIPTVIPEAPQWMKRTPWLMTNPAQFRPASPPELGSDIWARDYNEVKALGR
                     FDSQVRTSEQTEIARFWEEVMPPIYHGIVRSVATQPGREITQNARLFAAVTQASDDAL
                     IAVFDAKYHYGLWRPVTAIRNGDIDGNEATERDATWLPFISTPMHPEYPCAHCIVSAA
                     VGTVLEAEIGNNPSPILTTVSKAANNATRSWRNVHDFMQEVINARIYDGVHYRNSGEV
                     GSNMGRQIAQLAIKKYEVGEK"
     gene            complement(1581..2477)
                     /gene="hemF"
                     /locus_tag="PKZ49_11265"
     CDS             complement(1581..2477)
                     /gene="hemF"
                     /locus_tag="PKZ49_11265"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HND37184.1"
                     /translation="MNMIEVKAYLADLQNRIVAGLEAIDGKSFQRDGWERPDGGGGLS
                     CVIEEGNVLERGGVNFSYVFGHGLPASATAARPELAGRSFEAAGVSLVLHPRNPYAPT
                     VHMNVRFFEAKKEGADPVWWFGGGMDLTPYYGFEEDAIHFHQTCKTALDPIGSDYYPR
                     FKKWCDEYFFLKHRKEPRGIGGVFFDDFNQPDFSTCFKLTQRVGDHFLPAYAPILERR
                     LNVPYGEQERDFQAYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSLPPIVKWRYNW
                     TPQAGSPESKLYTDFLIGKNWI"
     gene            2620..3795
                     /gene="aroC"
                     /locus_tag="PKZ49_11270"
     CDS             2620..3795
                     /gene="aroC"
                     /locus_tag="PKZ49_11270"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HND37185.1"
                     /translation="MSGNTLGKLFCVTSFGESHGPAIGCVVDGCPPGLELSEADIQYE
                     LDRRKPGTSRHVTQRREADVVEILSGVFEGKTTGTPIGLLIRNEDQRSKDYSKISDTF
                     RPGHADYVYWQKYGIRDYRGGGRASARETAVRVAAGAIAKKWLNKTYGTLIRGYLSQL
                     GAISIPFKQWYDVNQNPFFVSDNDFVPQLEGYMDSLRKSGDSVGAKISVVAEGVPVGW
                     GEPVYDRLDAEIAYAMMSINAVKGVEIGSGFACVIQKGTEHSDEMTPNGFLTNNAGGI
                     LGGISTGQPITVNVAIKPTSSIRLGRRSIDKQGNPVIVETHGRHDPCVGIRATPIVEA
                     MLALLLIDHALRHRAQNADVYCSTPKIAGSIQQSNQTSVPVEDINLDHTENPDAEEA"
BASE COUNT         1073 a          911 c          821 g         1067 t
ORIGIN      
        1 acattaggac gaagattagt cgatggtgcc aaaattgtac ggatttttgg tcaagaaatt
       61 gttgttcgat cagatctcta caccattggc ggactttcag cgcacgcaga tcaagctggg
      121 attctaaatt ggctcaaagc atttaaaaca cctcccaacc atacctacat cgttcatggt
      181 gaaccgagta atgctaatgc cctagccaat atcataaaac aacaactcaa ctggcccgtt
      241 agtattccag aaagattcgc cacagtaacg ctttgatcgt tcaagatgat acaaacctac
      301 ttttcaccta cctcatattt ctttattgca agctgagcaa tttgccgacc catatttgag
      361 cccacttctc ccgaattacg ataatgcacc ccatcataaa tccgtgcatt aatcacttct
      421 tgcataaaat cgtgtacatt acgccagctc ctggttgcat tattcgctgc cttactcacc
      481 gtagtcagta ttggggacgg attatttcct atttccgctt ccaatacggt tccaactgca
      541 gcagaaacga tgcaatgcgc acacggatac tctggatgca tcggcgtact gataaatggt
      601 aaccaggtag catctcgctc tgttgcttca ttcccatcga tatcgccgtt ccgaatggct
      661 gttactggcc tccagagtcc atagtgatat ttagcatcga aaacagcaat gagcgcatca
      721 tcggaagcct gagttacggc agcaaataaa cgtgcatttt gtgtaatttc tctccccggt
      781 tgggttgcta cggaacgtac gatgccatga taaattgggg gcataacttc ctcccaaaaa
      841 cgagcaatct ctgtctgttc actcgttctg acctggctat caaatcgacc aagggctttt
      901 acttcattat aatctcgcgc ccaaatatcg cttcctaact caggtgggga cgccggccta
      961 aattgcgctg gattcgtcat cagccaaggc gttcgcttca tccattgtgg tgcctctgga
     1021 ataacagtgg gtatataaac acctgcacta gtattgggcc gataagactc tttgtcagca
     1081 gcgccatctt gggttcgctt tgctaatatc gcttgcgcta cttttgcacc taactcgata
     1141 cccgctcttt ttgctgaacc ctcgctaata gcagaaatag cagtctgata tgttttttca
     1201 attgaagctt gctgattagg tgctagctgc attaacgtaa ctcgattagc ttctgcaatc
     1261 gcagcttcaa ttgatgcatc ggggttaggt ggtagcaatt caatatcact tgtagagtaa
     1321 cgacgtgtga tggcattgac tgattcatag actgctgctt gaacaattgc taatgcgcgt
     1381 tcagcgggta aaggcccaat ttcagcatcg acaacgatat tacccgccca tttattccag
     1441 tctgttacag catcagcctt tgaatgggat atagacaata atactaagct aacaataatc
     1501 aaccaaccca atcgatctaa taaaaatgtt ttcttcatct agaaacctcc aagaaagtag
     1561 tttaaatcaa tagttctcaa ttaaatccag ttcttgccaa tcaagaaatc tgtatatagc
     1621 ttggattcgg ggctccctgc ttgtggcgtc cagttatagc gccattttac aatcggcggc
     1681 agtgacatca gaatggactc agttcgccca cccgtctgca agccaaataa agttccccga
     1741 tcccagacca agttgaattc cacataacga ccacgtcgat aagcctgaaa atcccgttct
     1801 tgttcaccgt aagggacatt taagcgcctc tccagaattg gagcatacgc aggtaaaaaa
     1861 tgatctccta ctcgctgagt caacttaaag caggttgaaa aatcgggttg attgaaatca
     1921 tcaaagaaaa caccgcctat tcctcttggc tcttttcgat gtttgagaaa aaaatattcg
     1981 tcacaccatt tcttaaaccg tggatagtag tctgatccaa tgggatcaag cgctgtttta
     2041 caggtttgat ggaaatgaat tgcatcctct tcaaagccat aataaggcgt taagtccatt
     2101 cctccaccaa accaccatac aggatcagca ccttcctttt tcgcttcaaa aaagcgtaca
     2161 ttcatatgga ctgtaggggc atatggatta cgtggatgca aaacgagtga cactcccgcc
     2221 gcttcgaaag aacgacctgc caactcgggg cgtgctgccg tcgcagaggc aggtaagcca
     2281 tggccaaaga catacgagaa attaacaccg ccccgctcta gtacattgcc ttcttcaatg
     2341 acacagctca gccctccgcc tccatcaggc ctttcccatc catcacgttg aaatgacttg
     2401 ccatcgattg cttctagacc tgctacaatt ctattttgca aatcagccag atatgctttg
     2461 acttcaatca tattcattct ttattccccc tcaatctact acactgcatt agttaatttc
     2521 tctattattc ctagggtaat tctaacctca gctcgaaagc tagggaagaa atttggttgc
     2581 tatgttagac ttactttact tatttcataa ataaaaggta tgtcaggcaa tacattagga
     2641 aaattgtttt gcgttacgtc gtttggtgaa tcgcatggcc ctgcaatcgg ctgtgtagta
     2701 gacggttgtc cacctggact cgagctatct gaggcagata ttcaatatga acttgatcga
     2761 cgcaaacctg gaacctcgcg tcatgtaacc caacgtcgcg aagcagatgt tgttgaaata
     2821 ctctctggtg tttttgaagg aaaaacaacg gggacaccga tcgggctcct aattcgaaat
     2881 gaagatcagc gaagtaaaga ctatagcaaa ataagcgaca ctttccgtcc tggccacgct
     2941 gattatgtgt attggcaaaa atatggtatc cgtgattacc gaggtggcgg tcgagcatct
     3001 gctcgcgaaa cagcggtaag agtggcggca ggtgcgatag caaagaagtg gttgaataaa
     3061 acctatggca cgctgattcg tggctacctg tcacagcttg gagcaattag catccctttc
     3121 aagcaatggt atgatgtcaa tcagaatccc ttttttgttt cagacaatga ctttgtccct
     3181 caacttgaag gttatatgga tagtctacgc aagtcaggtg attcagttgg cgccaagatt
     3241 agtgttgttg ctgaaggtgt gccagtcggt tggggagaac ccgtctatga tcggctcgat
     3301 gctgaaattg cctatgcgat gatgagtatc aatgcagtta agggtgtcga aatcggctca
     3361 ggattcgcat gcgttattca aaaaggaact gagcactctg atgaaatgac gccaaatggc
     3421 tttttgacaa acaacgctgg tggcattctg ggaggcattt cgactggcca gccgataaca
     3481 gttaatgttg cgattaaacc aacatcaagt atccggttgg gaagaagatc cattgataaa
     3541 caaggcaatc ctgtcattgt tgagacccac gggcgacatg atccttgcgt gggaattcgt
     3601 gcgacaccga ttgtagaagc aatgctggca ttgttgctta ttgatcatgc gctacgccac
     3661 cgtgcacaaa atgctgatgt ctattgcagt acacccaaga tagcaggcag tattcagcaa
     3721 agcaatcaaa cttctgtccc agtagaggat ataaatctcg accatactga aaacccagat
     3781 gctgaggaag cgtgatcatt ttctactggt tttacttttt agtcggtcct gcaaaattaa
     3841 cttaaccgtg gcggaggttt tgtatacaag aa
//
LOCUS       DAOQKQ010000208         3840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_14270_length_3840_cov_6.700661, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000208 DAOQKQ010000000
VERSION     DAOQKQ010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14270_length_3840_cov_6.700661"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..613)
                     /locus_tag="PKZ49_11275"
     CDS             complement(68..613)
                     /locus_tag="PKZ49_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HND37186.1"
                     /translation="MRKILSLICYLFLLSACTSLPDVIKNIPVIDVPYGVVAKDASTY
                     KNKQVRWGGVIIQTETEKELSFVQILFYPLDSSGYPLLNQEPEGRFIIASTDFLDPAI
                     YTKDRVITVAGMLNGGVERSIGNKKISIPLLLTKAIYLWPKDYRKSYISDGRYPYFYY
                     PGFYGYPFFYPGYYGPYRYWR"
     gene            869..1624
                     /gene="minC"
                     /locus_tag="PKZ49_11280"
     CDS             869..1624
                     /gene="minC"
                     /locus_tag="PKZ49_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HND37187.1"
                     /translation="MSLDVNKNYDMQPVLEFKSSTFFVLVLIIHSNDLMMIEQKLREK
                     TSQAQDFFRNSPIILDVRELNQQSHGIDIIKLINLIRSIGLFPVGIRGGNDQQNLQAA
                     SLFIPVDMSQSSASSQAEIQKTATSSPSAAPHEVIKEIVVSTATTLINQPVRSGQRIY
                     ASGDLIILTQVSAGAEIMAEGNIHVYNTLRGRALAGVQGNRDARIFCSDLQAELISIA
                     GDYKTSEDLKDITPRKPVQIYLQDQALIIKELI"
     gene            1651..2460
                     /gene="minD"
                     /locus_tag="PKZ49_11285"
     CDS             1651..2460
                     /gene="minD"
                     /locus_tag="PKZ49_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006891875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HND37188.1"
                     /translation="MARIIVVTSGKGGVGKTTTSAAIAMGLAKRGHKTAVIDFDVGLR
                     NLDLIMGCERRVVYDFINVINGEANLSQALIRDKNCNLLYILPASQTRDKDALSIEGV
                     GKVLEELAKDFQYIVCDSPAGIEKGAYLAMYYADDAFVVTNPEVSSVRDSDRMLGILA
                     SKSRRAELGMEPIKEFLLLTRYDPNRVESGEMLSLEDVQEILSLHLLGIIPESKSVLT
                     ASNAGVPVILDEKSDAGMAYADVVARYLGEKRPHRFIESKKGFLRKLFGGK"
     gene            2460..2738
                     /gene="minE"
                     /locus_tag="PKZ49_11290"
     CDS             2460..2738
                     /gene="minE"
                     /locus_tag="PKZ49_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HND37189.1"
                     /translation="MSLLDYFRSSKSKTASIAKERLQILVAHERYYRNKPSYLPQLQE
                     ELLEVIRKYVNVDQDAISVKFEQDENQETLELNIVLPESQNNKSPLEI"
     gene            2779..3420
                     /gene="lolB"
                     /locus_tag="PKZ49_11295"
     CDS             2779..3420
                     /gene="lolB"
                     /locus_tag="PKZ49_11295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HND37190.1"
                     /translation="MIIRVQETIYFVSCSILLSTILLLGGCASLEKHSVVKTIVLEPE
                     AEKARDLTNFDLIGRISVKTNNDGFSGGIRWQHKNHFHHLLLLSPIGQVVAQIAQDDQ
                     GIQLITSDQKIYYAASIEALTEQVFGWKLPIIGLEYWIMGRANPLIVAEIDMDRDDQV
                     IAIRQDGWEITYLDYSSRSVPQHNKVKSAPRIIELNYPNLKLKLVIDSWKNIN"
     gene            3468..>3840
                     /gene="ipk"
                     /locus_tag="PKZ49_11300"
     CDS             3468..>3840
                     /gene="ipk"
                     /locus_tag="PKZ49_11300"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634214.1"
                     /note="An essential enzyme in the nonmevalonate pathway of
                     isopentenyl diphosphate and dimethylallyl diphosphate
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HND37191.1"
                     /translation="MQFYPAPAKLNLFLHIIGRRDDGYHLLQTIFRFIDLNDRLGFNL
                     RNDGVIQLQTPLAGVAAEQNLCIRAAKLLQEEGSVNQGVDIYLEKHIAMGGGLGGGSS
                     DAATTLIMLNSLWGLNWHRDRL"
BASE COUNT         1247 a          702 c          782 g         1109 t
ORIGIN      
        1 gcaagaatta acagtaagaa gaaaaaactt agcccagcga tacgaatcaa tttctgcacg
       61 actgaatcta ccgccaatat cgatatgggc catagtagcc aggatagaag aaaggataac
      121 cataaaatcc aggatagtaa aagtaaggat atcttccatc gctaatataa gattttcgat
      181 aatctttagg ccatagataa atagctttgg tcaataacaa tggaatactg atcttcttgt
      241 ttccaattga gcgctcaaca ccgccgttta acatacccgc tactgtaatg accctatctt
      301 tagtatagat agcagggtct aaaaaatccg tgcttgcaat aataaatcgg ccctctggtt
      361 cttgatttaa tagtgggtac cctgaagaat caagtggata gaaaagaatt tgcacaaaac
      421 tcaattcctt ctcagtttca gtttgaatta tcacaccgcc ccaacgaacc tgcttatttt
      481 tgtaagtact cgcatcttta gccaccacac cgtaaggcac atcaataacc ggaatatttt
      541 taataacatc tggtagacta gtacaggcac taagtaggaa taagtaacaa attagagaca
      601 ggatttttct cataattgct tattaaaatt ttcttcaata atgattacca tcatagcctt
      661 tcattcgttg agacagcatg ataaatcaac tagttcgctt tgattttacg cttatttatt
      721 catcattaaa tataattaac cgaggaatat tttttcaatg agactctaca aggcaaaaac
      781 tagcccttag cgaccattgg aaggtataat tgccaacctg tattagagca ccacatattt
      841 aattcatgat gattgcaaga ttaaccggat gtcgctagac gttaataaaa attacgacat
      901 gcaacctgtg cttgagttta aaagcagcac tttttttgta ctagtgctga taatccatag
      961 taatgatttg atgatgatcg agcaaaagct tcgtgagaaa acatcacaag ctcaggattt
     1021 ctttagaaat tcaccaatca ttcttgatgt acgtgagctt aatcaacaaa gtcatggaat
     1081 tgatatcatc aagctcatta atctgatacg cagtatcggc ctatttccag ttggaattcg
     1141 aggggggaac gatcagcaaa atcttcaagc ggcttctcta ttcattcccg tagatatgtc
     1201 acaaagcagc gcaagttctc aagctgagat acaaaaaacg gctacctcat caccatcagc
     1261 cgctccacat gaagtcatca aagaaattgt tgtaagtact gcgactacac tgattaatca
     1321 accggttcgt tcaggtcagc gcatctacgc ttcaggagat ttaattattt taacgcaagt
     1381 cagcgcaggc gctgaaataa tggctgaagg aaacatccat gtttacaata cattaagggg
     1441 tagagcctta gcaggtgtgc aaggaaacag agacgctcga atcttttgct ctgatttaca
     1501 agcagagctg atttctatcg cgggggatta taaaaccagc gaagatctaa aagatataac
     1561 cccgaggaaa ccggtacaaa tctatttaca agatcaggca ttgattatta aagagttaat
     1621 atagcttcat ttttagcaaa ggagagtcaa ttggctagaa tcatcgtcgt aacatcaggc
     1681 aaaggaggcg ttggcaaaac aacaacgagc gcagcgattg ccatggggct tgcaaaaaga
     1741 ggacacaaga ctgctgtaat tgatttcgat gttggtttac gcaatcttga tttaattatg
     1801 ggttgtgaac gacgagttgt ttatgatttt attaatgtaa tcaatggaga ggcaaatcta
     1861 agtcaagctt taattcggga taaaaactgt aatttgcttt atatcttgcc cgcatcacaa
     1921 actcgggata aagatgcttt aagtatagag ggtgtcggta aggtgctcga agaattagcc
     1981 aaagattttc aatacattgt ttgcgattct ccagccggta tcgaaaaagg ggcttactta
     2041 gccatgtact acgcggatga tgcatttgtg gttaccaacc ctgaggtctc atctgttaga
     2101 gattcagatc gcatgcttgg aattttagcc agcaaatcaa ggcgcgctga actggggatg
     2161 gagccaatca aagaattttt gttgttgact cgctatgatc caaatcgagt ggagtccgga
     2221 gaaatgctta gtctggaaga tgtgcaagaa attttgtctt tgcacctgct tggtatcatt
     2281 ccggaatcaa aatcggttct tacagcatca aatgcaggcg taccggtcat tctagatgaa
     2341 aaaagtgatg cgggtatggc ttatgctgat gttgttgctc gatatctcgg tgagaaaaga
     2401 ccacatcgct ttatcgaaag caagaaagga tttctcagga agctttttgg aggaaaataa
     2461 tgagtctact cgattacttt agatcatcta agtctaaaac agcgtctatt gccaaagaaa
     2521 gattgcagat tcttgtagcg catgagcgtt attatcgaaa taaaccttcc tatttacctc
     2581 agttgcaaga agaattactt gaggtaatac gaaaatatgt caatgttgat caagatgcca
     2641 tctcagtcaa atttgaacaa gatgaaaatc aagaaacact cgaacttaat attgttttgc
     2701 ctgaatctca aaataacaaa agcccattag aaatttaata tatcagggtt atctgataaa
     2761 aaaacgacta acgctatttt gattatccgt gttcaagaaa cgatatattt tgtatcgtgt
     2821 agcattttgt taagtacgat tctgttactt ggaggctgtg cttcattgga gaagcacagt
     2881 gttgtcaaaa cgattgtttt agaaccagag gcagaaaaag cacgcgactt aactaatttc
     2941 gatttgatcg gaagaatttc tgtcaaaaca aataatgacg gattttcagg tggaattcgt
     3001 tggcagcata aaaatcattt ccatcatctg ttactactct cgccaattgg ccaggttgtt
     3061 gctcagatag cacaggatga tcaaggaatt cagctcataa cctcagatca gaaaatatac
     3121 tatgcagcga gtatcgaagc gttgaccgag caagtattcg gttggaaact tccgattata
     3181 ggacttgagt attggataat gggcagggcg aatcccctga tcgttgcaga aattgacatg
     3241 gatcgcgatg atcaagttat tgcgataagg caagatggtt gggaaataac ttatttggat
     3301 tattcttctc gctcagtacc acaacataat aaagtaaaaa gcgcccctcg aataatcgaa
     3361 ttaaattacc ctaacctaaa attaaaactc gtcattgata gctggaaaaa cattaattga
     3421 cgatcatctc agtgataaga tgctattctg cgaactaaca aaataatatg caattttacc
     3481 cagcacccgc taaactcaat ttgtttttgc atattatcgg tcggcgtgat gatggatatc
     3541 atctattaca aactatcttt aggtttattg atctcaatga tcgattaggt ttcaatttaa
     3601 gaaacgatgg tgtcatccaa ttacaaactc cgctagcagg ggttgcagca gagcagaatt
     3661 tatgtattcg agctgcaaaa ttacttcagg aagaaggttc tgtaaatcaa ggtgttgata
     3721 tttatcttga gaaacatatt gcaatgggtg gagggttagg tggtggaagc tcggacgcag
     3781 cgacgacgtt aatcatgctt aattctttat ggggtttaaa ttggcacagg gatcgtttaa
//
LOCUS       DAOQKQ010000209         3840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_14272_length_3840_cov_4.878203, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000209 DAOQKQ010000000
VERSION     DAOQKQ010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14272_length_3840_cov_4.878203"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="PKZ49_11305"
     CDS             complement(<1..815)
                     /locus_tag="PKZ49_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HND37192.1"
                     /translation="MKKSLFLVAWVISITLSLPLHAANLMEIYREALEHDAQYGSARA
                     AYIAAQEKLPQGRAGLLPTINATGTAQNQYIDTNLGSEVLIRNRGVVIAATQPLFRIE
                     NFIIYEQSKNQVAQADARFVLAAQDLIIRVAQAYFNALIAQSNLEVVETQKKAIQQQL
                     EQAKRNFEVGVSTIVDTHEAQARYDLTLSQEIAARNALEISKRTLQGIINRFPDSLVR
                     ISIDKIITDKLDLAHDNMEEWVNIAEQKNFSLRVQQIAFDLAEQEISKAKAGH"
     gene            complement(885..1556)
                     /locus_tag="PKZ49_11310"
     CDS             complement(885..1556)
                     /locus_tag="PKZ49_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HND37193.1"
                     /translation="MKQNTQIDIEKTRFNMVEQQIRTWNVLDDTILNLLYKIHREEFV
                     PAAYRALAFTDMEIPLEHGAVMLTPKMEARIVQELHIKKTDKILEVGSGSGYMTALLA
                     ELGAHVHSVEIVPTLCAMAKINLQTHDITNVTIEQGDASQGWPNHAPYDVIVLTASTP
                     VLPEAFQKSLNPGGRLFAIVGEDPVMEAILITRTASDTYQTTHLFETCTAPLQNAQQP
                     PRFTF"
     regulatory      1620..1724
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1759..3654
                     /gene="thiC"
                     /locus_tag="PKZ49_11315"
     CDS             1759..3654
                     /gene="thiC"
                     /locus_tag="PKZ49_11315"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HND37194.1"
                     /translation="MNTIISKLETFTSETAQVDQAAVKPLPNSRKIYISGSSSDIRVP
                     MREISQTDTVTQTGAEKNPSIYVYDTSGPYTDPDIAIDIRNGLYPLREKWIEARGDTE
                     LLSGLTSAYSQERLNNPKLSELRFNLKRASRRAKSGTNVTQMHYARQGIITPEMEFIA
                     IRENQHWEALKNNNQQDLIRQHPGQDYGAAIPRLITPEFVRDEVARGRAIIPANINHP
                     ESEPMIIGRNFLVKINANIGNSALGSSIQEEVEKMTWAIRWGGDTVMDLSTGKNIHET
                     REWIIRNSPVPIGTVPIYQALEKVDGKAEELTWEIFRDTLIEQAEQGVDYFTIHAGVR
                     LPFIPLTAKRMTGIVSRGGSIMAKWCLAHHKESFLYTHFEEICEIMRAYDVSFSLGDG
                     LRPGSIYDANDEAQFAELKTLGELTQIAWTHDVQVMIEGPGHVPMHLIKENMDLQLKY
                     CQEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPDKDDVK
                     DGIIAYKIAAHAADLAKGHPGAQIRDNALSKARFEFRWSDQFNLSLDPDKAKQFHDET
                     LPQEGAKLAHFCSMCGPSFCSMKITQDVRNYAAQQGIAEDEALKHGMAQKSSEFKEKG
                     AEIYGKL"
     gene            3681..>3840
                     /locus_tag="PKZ49_11320"
     CDS             3681..>3840
                     /locus_tag="PKZ49_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HND37195.1"
                     /translation="MGWALLLKALILGLVEGLTEFLPVSSTGHLILIGDLLNFNDEKA
                     KVFTVAIQL"
BASE COUNT         1077 a          769 c          866 g         1128 t
ORIGIN      
        1 tagtgccctg cttttgcttt actaatttct tgctcagcta aatcaaaggc aatttgctga
       61 actctaagag aaaaattctt ttgttctgca atattgaccc attcttccat attgtcatgg
      121 gctaaatcca atttatccgt aatgatctta tcaatgctga ttcttaccaa gctgtccggg
      181 aatcgattaa tgataccttg caaagtacgc ttgctaattt ctagggcatt tcttgccgca
      241 atttcttgag aaagtgttaa atcatagcga gcttgcgctt catgggtatc aacaatagtg
      301 gatacaccta cttcaaaatt gcgttttgct tgttctagtt gttgctgtat tgcttttttt
      361 tgtgtctcaa ctacctctaa gttcgattgt gcaatcaagg cattgaaata agcttgagcg
      421 acacggatga tcagatcttg tgcagctaat acaaatcttg cgtcagcctg agctacttga
      481 ttttttgatt gctcatagat aataaaattt tcaattcgaa acaaaggttg agttgcagca
      541 atgactacac ctcgatttct gattaatact tctgatccaa gattggtatc gatatattga
      601 ttttgtgcag ttccagtcgc gttgatcgtt ggcaataaac cagctcttcc ctgaggtaac
      661 ttttcttggg cagcgatata agctgctcgc gctgatccat attgagcatc atgttcgagt
      721 gcttcccgat aaatctccat tagattggcc gcatgtaaag gcagtgataa cgtgatactg
      781 attacccatg caactaaaaa aaggcttttt ttcattaatt agttagttaa ggtaacgatc
      841 atggagggct atcgattgct gttgatgaca ttaaaagtga atgactaaaa agtaaatcga
      901 ggtggttgct gggcattctg cagtggcgct gtacaggttt caaataaatg ggttgtttgg
      961 taagtatctg atgccgtgcg agtaatcaat attgcttcca taacaggatc ttcgcctaca
     1021 attgcaaaca atcttccacc gggattcaag cttttttgga aagcttccgg tagcacaggg
     1081 gttgaagcag ttaacacaat cacatcatag ggtgcatgat taggccaacc ttgtgaagcg
     1141 tctccttgtt cgatcgtgac attagtaatg tcatgtgttt gtagattaat tttcgccatg
     1201 gcgcatagag tcggtacaat ttcgacacta tgaacatgtg cacctaattc tgcgagtaac
     1261 gcggtcatgt acccactccc actgcccact tcaagaattt tgtcagtttt tttgatatgt
     1321 aattcttgaa cgatgcgagc ttccatttta ggcgttagca ttacagcccc atgctcaagt
     1381 gggatctcca tatccgtaaa agcaagcgca cgataggctg ctggaacaaa ttcttcacga
     1441 tggattttat agagcaaatt aagaatggta tcgtccaaaa cattccaggt acgaatttgt
     1501 tgttcaacca tgttgaatcg ggttttttcg atatcgattt gggtattttg tttcatagtt
     1561 ggcattgagg ataaaaaaga atcagacttg caattatttc atatttccat tagtttcaca
     1621 gcttaagcta ggggtccgca ttttgattat taaattgtcg gtgagaaagt cccttaatac
     1681 ctgatgcgga taatgccgcc gtagggaagc tgaagcgatt actccttctg ctccttgcga
     1741 attgatatgg agcataccat gaataccatc atttcaaagt tagaaacatt tactagcgaa
     1801 acggcgcaag tcgaccaggc tgctgttaaa ccgttaccca attctcgtaa gatctatata
     1861 tcagggtcaa gttcagatat tcgggtacct atgcgtgaaa tttcacaaac ggatacagtg
     1921 actcaaacag gcgctgaaaa gaatccctcg atttatgtgt atgacacatc tggtccttat
     1981 accgatcctg atattgcaat cgatattcgc aatggccttt atcccctgcg tgaaaaatgg
     2041 atagaagccc gcggcgatac agaattactt tcagggctga cctcggctta tagtcaagaa
     2101 cgattaaata atcccaaatt atcagaattg cgcttcaatt tgaaacgcgc ttcgcgcaga
     2161 gcaaaatctg gaacgaatgt cacgcagatg cattatgctc gacaagggat tattacacct
     2221 gaaatggaat ttattgctat tagggagaat cagcattggg aggcattgaa aaataataat
     2281 cagcaagatc tgattcgcca acacccaggc caagattatg gtgcagcgat tccacgcctg
     2341 attacccctg agtttgttcg cgatgaagtg gcgcgtggca gagcaatcat tccagccaat
     2401 atcaatcacc ctgaatctga accgatgatt atcgggcgta atttcctggt taaaatcaat
     2461 gccaatattg gaaattctgc gctgggctcg agcattcaag aagaagtaga aaaaatgact
     2521 tgggcgattc gctggggtgg agatacggtg atggatcttt cgacgggtaa gaatattcat
     2581 gaaactcgcg agtggattat tcggaatagc ccagtgccga tcggtacggt tcccatttat
     2641 caggccttag aaaaagtcga tggtaaggca gaagaactta cttgggagat tttccgagat
     2701 accttgattg agcaggctga gcaaggtgtg gattatttta cgatccatgc tggagtacgg
     2761 ttgcctttta taccgctcac tgcaaagcgc atgacgggaa ttgtttctcg gggcggctcc
     2821 attatggcca aatggtgctt agcgcatcat aaagaaagtt tcttgtatac gcattttgaa
     2881 gaaatttgcg agatcatgag agcttatgat gtgagctttt cactgggtga tggtttacgt
     2941 ccaggctcga tttatgatgc caatgatgaa gcacaatttg ctgaactaaa gacgctaggt
     3001 gaattgactc aaatcgcctg gacgcatgat gtgcaagtaa tgatcgaagg ccctggccat
     3061 gttcccatgc atttgatcaa agagaacatg gatttacagc ttaaatattg tcaagaagca
     3121 cctttttaca cgctaggccc actcactacg gatatcgctc ctggctatga tcatattacc
     3181 tccgcgattg gtgcagcaat gatcggctgg tatggtacag ccatgctgtg ttacgtgacg
     3241 cccaaggaac atttggggtt accggataaa gatgacgtca aggatggaat tatcgcttac
     3301 aaaatcgctg cccatgcggc agatctagcg aaagggcatc ccggtgcaca aatacgagat
     3361 aacgcgcttt ccaaagcacg gttcgaattt cgctggagtg atcagttcaa tttaagcctt
     3421 gatcctgata aagcaaaaca gtttcacgat gaaacgctac cgcaagaagg ggcgaagctg
     3481 gctcattttt gttctatgtg tggcccaagc ttttgctcga tgaaaattac ccaagatgtg
     3541 cggaattatg ctgctcaaca aggcatcgca gaagatgaag cacttaagca cggtatggct
     3601 cagaaatcga gtgaattcaa agaaaaaggg gctgaaattt atggaaagct gtgagcaatt
     3661 tttctgctta gcataaaatc atgggatggg cgctactact taaagcattg attcttggcc
     3721 tcgttgaggg gttaaccgag tttcttcccg tttcctcaac aggtcatttg atcttgattg
     3781 gggatctact gaattttaat gatgaaaaag ccaaggtttt taccgtagcg attcagcttg
//
LOCUS       DAOQKQ010000210         3759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_14821_length_3759_cov_5.867981, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000210 DAOQKQ010000000
VERSION     DAOQKQ010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14821_length_3759_cov_5.867981"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..990)
                     /locus_tag="PKZ49_11325"
     CDS             complement(214..990)
                     /locus_tag="PKZ49_11325"
                     /inference="COORDINATES: protein motif:HMM:NF025774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HND37196.1"
                     /translation="MSNNESVAESLLNIKQAAKILNASEISLRRWSDAGKLPCMRIGV
                     KRERRYRLTDLLAYLEQDQAPVNIATFGHATSRIAHIDLEGIAINYGSHLCAFYDTDL
                     GRLKLALPFLLDGLQSGNRCFLVATKEVQSIIINELCDVRSCIDQDIQDGRIVLTEGM
                     ANSTELYTFFENAFMDANKQGIQGLRVLGDMAWTLQKGMGIAELNDFESRYNQGLGHR
                     FPVVSLCQYDARLFSGTAILGALKCHDDTFHYPLTHFLGS"
     gene            1246..2511
                     /locus_tag="PKZ49_11330"
     CDS             1246..2511
                     /locus_tag="PKZ49_11330"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HND37197.1"
                     /translation="MNPVSGSSEDYSFYSALSHPPLRAAGNTPDLSIYRPQGGPNYGA
                     FRFRAPVEGLLQIGGTHNAKTDWSDDFEIAWTKMGSKGPLVLFLHGVPTNRSQWEEVQ
                     GQVSRFCETISIDMLGMGESSKPRLYGRKDNSSPNAMWHWINDVDYIEKLMQQEYPRR
                     QFIFIADDWGSGIASHYAAKHNDRLNALIQMDPIAFDGYPVNEIQAIGRASEIPNTPE
                     GDAQFAMLFAAFDQTLVQILKSMVYDPSKYNQYNLRYLTFPYVDVDYERNGKNDGVTD
                     VARSTTMRLKLHNLRVLSDRAAILSPSLLLPYHPQSNPHGVQYQNITAPTLIMWGEYD
                     NMMPAAQTQRFAHVLGTDDVQITYIPRAGHFPATDNPDCVTDTILNFIRRVMGRSALA
                     DIYIGNQGIWKGDERQMIQELRVLHGIPD"
     gene            2571..2789
                     /locus_tag="PKZ49_11335"
     CDS             2571..2789
                     /locus_tag="PKZ49_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDCCVxC domain-containing (seleno)protein"
                     /protein_id="HND37198.1"
                     /translation="MKTIMLESDLTCPYCGFIKQETMPTNACLYFYECTSCNTLLKPK
                     PGDCCVFCSYGTVVCPPRQAEASCCNFS"
     gene            2801..3370
                     /locus_tag="PKZ49_11340"
     CDS             2801..3370
                     /locus_tag="PKZ49_11340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HND37199.1"
                     /translation="MIEGNNDLNIQTWLEEHGDYLFSYALLRVKDTHLAEDLVQETLL
                     AAITAKNTFSNQSTIRTWLIGILKHKLVDQFRRQGREVGIGDLIDQDEGDNLDNFFRV
                     NGRWIEKPDAFPNPESAFQQKEFWKVFQQCLSGLKPKQVEVFLAKEIHGMSNKDICKD
                     FALSQTNIWVIMHRARLSLIKCLKINWID"
BASE COUNT         1159 a          791 c          765 g         1044 t
ORIGIN      
        1 aagatgtgct aaccgtagag ggttctgatt atcgttcatt gcgagaagaa gggttatttg
       61 cgtcaatatc cggtgaaata agcgccattt ttaggaagcc cagaacattt gtaaaggaag
      121 atggcggatt gagtagacaa aggaggcgcg cacgctgatc tgaatttcta cagaggtact
      181 acaatcacga agatagttga cagtcatcca aactcaacta cccagaaaat gagtcaaagg
      241 atagtggaaa gtatcatcat gacacttaag agcacccaat attgcagtac cagaaaataa
      301 tctggcgtca tactgacata aactaacaac tggaaatcgg tgcccaagtc cctgattgta
      361 tcgactttca aaatcgttta actctgcaat tcccatacct ttttgtaacg tccacgccat
      421 atcaccgagt actcgcaaac cttgaatacc ttgtttgttg gcatccataa aagcattctc
      481 aaagaaagta tataactctg tgctgtttgc cataccctcc gttaaaacaa tgcgaccatc
      541 ctgaatatcc tgatcaatgc atgatctgac atcacatagc tcattgatga taatagattg
      601 aacttccttt gtagcaacca gaaaacagcg attgcctgat tgaagaccat cgagcaaaaa
      661 tggtaacgct aatttaagtc gtcccaaatc tgtatcataa aacgcacata agtggctgcc
      721 ataattaatt gcgataccct caagatcaat atgagcgatt cggctggtag cgtgcccaaa
      781 tgtagcaata tttacaggcg cttgatcctg ctcaagataa gccaagagat cagttaagcg
      841 atatctcctc tcgcgcttga ctcctatgcg catacaaggc agcttgcccg catcactcca
      901 gcgtctcaaa gatatctcac tggcattcaa aattttagct gcttgtttaa tgtttagaag
      961 cgactcagct acactttcat tgttgctcac gcacattctc tcctaaaata attcccattt
     1021 atctcagcag aatttttagg tttgtggtct tttcttatag ttctcaatta catcctctat
     1081 agttatctta agtaacttcc ctgtatttat aaaactcttg acttttatta tagcatgggt
     1141 caaattataa aaaagatcat tgatgattat cattgatcat tattgatgac gtatatcatc
     1201 agatactcac agcacaaatc aaatcaataa atacaggata acaaaatgaa tccagtttcc
     1261 ggaagctcag aggattacag tttctatagt gcactcagcc atccaccttt acgggctgca
     1321 ggtaataccc ccgatttgtc tatctaccgc ccacaaggtg ggcctaacta tggtgcattt
     1381 cgtttcaggg cgccagttga gggtttgctg caaataggtg gcacacataa tgctaaaact
     1441 gactggtcgg atgatttcga aattgcatgg actaaaatgg gtagcaaagg tcctttggtt
     1501 ctctttctac atggtgttcc aaccaatcgt tcgcagtggg aggaagtaca agggcaagtg
     1561 tctcgctttt gtgaaacgat ttctatcgat atgctgggga tgggtgaaag ttccaaacca
     1621 cgcctatatg gccgcaaaga taattcatcc cctaatgcga tgtggcattg gattaatgat
     1681 gtcgactaca ttgaaaaatt gatgcaacaa gaatatccga ggcgacaatt tattttcatt
     1741 gccgatgact ggggaagcgg tattgcttcg cactatgccg ccaagcataa cgatcgcctg
     1801 aatgcactga tacagatgga tcctattgcc ttcgatggct atccggtgaa tgaaatacag
     1861 gcaataggta gagcctccga aattccgaat acaccagaag gcgatgcaca atttgcaatg
     1921 ctgttcgcag catttgatca gacattagta cagattctaa agtcgatggt gtacgatcct
     1981 tccaaataca atcaatataa tcttcgttat ctgacttttc cttatgttga tgtggactat
     2041 gaacgcaatg gaaagaatga tggtgtcacc gatgtcgcca gatcaacaac catgcgccta
     2101 aaactccata atttgcgcgt actgtcagat cgcgcagcta ttctcagtcc atccttgcta
     2161 ctgccatatc atccgcaatc caatccccat ggtgttcaat accagaacat taccgctccg
     2221 acactgatca tgtggggcga gtatgacaac atgatgcctg ctgcgcagac gcaacgattt
     2281 gctcatgtat taggaacaga tgatgtgcaa atcacttaca ttccaagagc aggacatttt
     2341 ccggccactg ataatccaga ctgcgtcaca gacactatcc ttaattttat tcgtcgtgtt
     2401 atgggacgca gcgcgctggc tgatatctat attggaaatc agggtatctg gaaaggcgat
     2461 gaacgccaga tgattcaaga attacgggtg ctgcatggca ttcccgattg ataaagcagc
     2521 aaatttcatg atagtaattg ctggacattc catattttat gaacgattta atgaaaacaa
     2581 ttatgcttga atcggatttg acttgcccgt attgcggttt cattaaacaa gaaactatgc
     2641 ccactaatgc ctgtttatat ttttatgaat gcacaagctg caacacacta ttaaaaccga
     2701 aaccgggtga ctgctgcgtc ttctgttcct atggaaccgt ggtatgtcca cccaggcaag
     2761 ctgaagctag ctgctgcaac ttttcttgat aagtttcttt atgattgaag ggaataacga
     2821 cttaaacata caaacatggc ttgaggagca tggtgattat ctattcagct atgcactttt
     2881 aagagtaaag gatacacatc ttgcagaaga tctggttcaa gaaacactac ttgcagcgat
     2941 aactgctaaa aatacttttt ccaatcaatc aacgattcgg acctggctga tcggcatact
     3001 taaacacaag ctagttgacc aattccgacg acaggggcgt gaagtcggca ttggcgatct
     3061 gattgatcag gatgagggag acaatctgga taattttttt agagtcaatg gccgatggat
     3121 tgaaaaaccg gatgcttttc ctaacccgga gtcagccttt caacaaaaag aattctggaa
     3181 agtttttcag caatgcttat ctggactaaa acccaagcaa gttgaagtat ttcttgctaa
     3241 agaaattcat ggaatgagca acaaggacat ttgtaaggat tttgcactca gtcagactaa
     3301 tatatgggtg attatgcatc gtgcccgatt gtccttgatt aagtgtttaa agattaactg
     3361 gatagactaa tagtgggtaa attaaatgtt gaattgtaaa caagccagcc agttggcctc
     3421 tcgatcaatg gatgaaaaat tacccttgtg gaaaaacatg gcgttaaaga cgcacctgat
     3481 tttatgccgc agctgcacta attttacgcg gcaacttcac tttttacgca aagcctcacg
     3541 tcgatctaaa actaatcttg actttcagtt aactgacgaa gcaaggcaac gtatcgccaa
     3601 tgctctgaag gataagcaaa acggttaact gtcgcattaa ggagatacca agaaggattt
     3661 ggtcacacat cgtatgtgat ctttaaacaa aaatggtaac aagaaatgat aatgaatgag
     3721 gagaacaaat aatgttacaa gcagtcactg aaaaaacat
//
LOCUS       DAOQKQ010000211         3672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_15449_length_3672_cov_3.662980, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000211 DAOQKQ010000000
VERSION     DAOQKQ010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15449_length_3672_cov_3.662980"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="PKZ49_11345"
     CDS             <1..948
                     /locus_tag="PKZ49_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HND37200.1"
                     /translation="FTCRKENLYMAHNHSPDHDHSHVPKNFHRAFAIGIALNAGFVVV
                     EIVFGLFANSLALLADAGHNLSDVLSLLFAWGASVLVRKLPTQRHTYGLRRTSILAAL
                     INALVLLVAVGAIAWEAILRFGHPEAVASDTVIWVASVGIVINGFTAWLFMSGQKQDM
                     NIRGAYLHMMADAAVSLGVVLAAIAIKFTDWLWLDPFMSLVVAVIIGIGTWGLLRESL
                     SLALDAVPANIHPSKVEAYLASLPGIEAVHDLHIWAMSTTEIALTVHLVKPDAVIDDL
                     LLVQINNELRELFGIQHTTVQFERGDTSHPCKQAPAEVV"
     gene            1176..2345
                     /locus_tag="PKZ49_11350"
     CDS             1176..2345
                     /locus_tag="PKZ49_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HND37201.1"
                     /translation="MNFGKTLFAQLMEFVPWTSFARIVARHGGDSGVRNLSCTEQFRA
                     MAFAQLTYRESLRDIEACLLANQAKLYSMGFRAPVKRSTLADANEARNWHIWEDLATL
                     LIRRARKLYCNDSFGVDLTNTVYALDATTIDLCLSLFPWALFRTSKAAVRLHTLLDLR
                     GNIPAFIHITNGKTHEVNVLDTLSFEAGAFYVMDRGYLDFSRLFTLHQSGAFFVIRAK
                     RGMNAHRVYSMKVDRSTGIICDQRIVLDGFYISQDYPEQLRRIRFKDPETGKTLVFLT
                     NNTALPALTIAALYKSRWQVELFFKWIKQHLRIKRFIGNSENAVKTQIWCAVATYVLI
                     AIVKKELQIDASLYTLLQILSVSVFEKNQLQQALQGAVYIPEQCGDANQLNLLNF"
     gene            complement(2539..>3672)
                     /locus_tag="PKZ49_11355"
     CDS             complement(2539..>3672)
                     /locus_tag="PKZ49_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03790 family protein"
                     /protein_id="HND37202.1"
                     /translation="MPEDIAVIVNDDDPLSRQIGDYYQRARQIPKTNVIHLNFPPNRS
                     TMSSNEFIKLKAKIDRLTPEHVQAFAVAWTAPYRVDCMSLTSALAFGFDKKYCSDKCE
                     PTAPSPYFNASGPHPFDDYQVRPAMMLAGNSFSQVKDLIDRGIASDHTFPKGQAYLLN
                     TSDKARSTRASVFAKIAKEFSGVFPVQALEADFISERRDVLFYFTGLTEVTLLDTLEF
                     IPGALADHLTSAGGMLTDSPQMSSLRWLEAGATASYGTVVEPCSFPQKFPAPAVAMFH
                     YITGTSAIEAYWKSVAWPGQGIFIGEPLAKPFAPLLREINPGRFELKIFSPRTGRLQI
                     EKSISAAGPFKLVSQSQAIRRGLNRFYFRFNEKTDGYLKLQWN"
BASE COUNT          902 a          874 c          933 g          963 t
ORIGIN      
        1 ttcacatgtc ggaaagagaa tctatatatg gcacataacc actcccccga tcatgatcat
       61 agccatgttc ccaaaaactt tcatcgcgca ttcgcaatag ggattgcgct aaatgcaggt
      121 ttcgttgtgg ttgaaattgt ttttggttta tttgcaaatt ctctcgcgct gttggcagat
      181 gcaggccata acctcagcga tgttttgagt ttattattcg cgtggggcgc aagcgtctta
      241 gtgcgcaagt taccaacaca gcggcatact tacggtttgc gccgtacttc tattttggct
      301 gcattgatta atgcgctagt tttgcttgtt gctgttggcg ctatcgcctg ggaagccatt
      361 ctcagatttg gccatcccga ggcagtggca agtgacacag tcatctgggt ggcatcagtt
      421 ggcatagtca tcaacgggtt cacggcatgg ctgttcatgt ccggccaaaa gcaagatatg
      481 aatattcgcg gtgcatattt gcacatgatg gctgacgcag cagtttctct tggcgtcgtt
      541 ttggcagcta ttgccataaa atttacggac tggttgtggt tggacccatt catgagcctt
      601 gtggtcgcag taattattgg tattggcact tggggcttgc tccgtgaatc gttaagcctt
      661 gctcttgatg ctgttccagc aaatatccat ccgtccaaag ttgaagctta cctcgctagt
      721 cttccaggga ttgaagcagt tcacgaccta catatctggg caatgagcac gacggaaatt
      781 gcactaaccg tgcatctagt caagccagat gcggtcattg atgatttgct attggttcaa
      841 atcaataatg aattgcgtga gctgtttgga attcagcata ccaccgtcca atttgagcgc
      901 ggagatacat cccatccctg taagcaagca ccggcagaag tggtttaaag tagaaatgag
      961 taacttttgt tttatcataa taataaataa ttatttgaga gctccgcagg ttttgtaaac
     1021 ttaatcagta gtgtccggtt aaattagcta ctgaaacgct agagccaagt actgaagcgg
     1081 ctctccgaga aaatattttg gtgccgattt gaaatcatat tttacaaatt caggttcgag
     1141 taatctccag aagttgaacc ttctggaggt gatctatgaa ctttggaaaa acgctgttcg
     1201 cacaactcat ggagtttgtg ccatggacga gcttcgcgag aatcgtggct cgtcatggtg
     1261 gcgattcggg cgtgcgcaat ctgagttgta ccgagcaatt ccgcgccatg gcttttgcgc
     1321 agctgaccta ccgcgagagt ctgcgagata tcgaagcctg cttgctggcc aaccaggcga
     1381 agctgtacag catgggtttc cgcgcgccag tcaaacgttc gacactggcc gacgccaacg
     1441 aagcacgcaa ctggcacatc tgggaagact tggctacatt gctgatccgg cgtgcacgca
     1501 aactgtattg caacgacagc ttcggtgtcg atctgaccaa tacggtctac gcactggatg
     1561 cgacgaccat agacctgtgc ctgtcgctgt ttccatgggc actgtttcgc accagcaagg
     1621 cggcggtcag attgcatacc ctgctggatt tgcgcggcaa tattccggct ttcatccaca
     1681 tcacgaatgg caagacccac gaagtcaacg tgctggatac cttgtccttc gaggcagggg
     1741 ctttctacgt gatggatcgg ggttatctgg atttcagtcg cctgttcacg ctgcatcagt
     1801 cgggggcgtt cttcgtaatt cgcgccaaac gaggaatgaa cgcgcacagg gtgtactcga
     1861 tgaaggtcga tcgaagtacc ggcatcatct gcgatcagcg catcgtgctc gacggtttct
     1921 acatctcgca ggactatccc gaacaactga ggcgcatccg cttcaaagac ccggaaaccg
     1981 gcaagacgtt ggtgttcctg accaacaaca cagccttgcc cgcactgacg attgccgcgc
     2041 tgtacaaaag ccgctggcaa gtcgagctgt tcttcaagtg gatcaagcaa catctgcgta
     2101 tcaaacgttt catcggcaac agcgagaatg cggtgaaaac gcagatctgg tgcgccgtcg
     2161 ccacttacgt gctgatcgcc attgttaaaa aggagcttca aattgatgcc tcgctctaca
     2221 ctttgctaca gattttatcg gtctctgttt ttgagaaaaa ccagttgcaa caagccttac
     2281 aaggcgccgt ctacattcca gaacagtgcg gcgacgctaa ccaactaaat ttgttaaact
     2341 tttaaccgga cactactgac tgaatatttg cattaataaa cagtaatttg tatgtatata
     2401 aatcctgttg catttgacgg gtatttcgtt aaaaagttta ggggattttc gcgatttgtt
     2461 gcgcgatatt aaataacttc agcgcgcgca gatatgctgt gtgcactaat acctgattga
     2521 cggattagaa ttgattggtt aattccactg caacttcaaa taaccatctg ttttttcatt
     2581 aaaccggaaa taaaatcgat tcaatccgcg gcgaattgct tgcgattgtg aaaccagttt
     2641 gaacggtccg gcggcggaaa ttgatttctc tatttgcaat cgtccggtac gtggcgagaa
     2701 aatcttcaat tcaaaccgtc ctgggttaat ttcccgtagc aatggcgcaa atggcttagc
     2761 cagcggttca ccgatgaata ttccttgtcc cggccaagcg acacttttcc agtaagcctc
     2821 aatcgcgctg gttcctgtta tatagtgaaa catggcgacc gcaggagctg gaaatttttg
     2881 cggaaaactg caaggctcga ccacagtgcc gtagctcgcg gttgcgcccg cctccagcca
     2941 acgcaaactg ctcatttgtg gagaatcggt aagcatgcca cctgcggatg tcagatgatc
     3001 ggccagcgca cctggaatga attcgagcgt atccagcagg gtaacttcgg tcaatccggt
     3061 aaaataaaaa agcacatccc gccgctccga aatgaaatcg gcttccagtg cctgcacggg
     3121 aaaaacaccg gaaaattctt ttgcgatttt cgcaaaaacc gatgcgcgcg tgctacgtgc
     3181 tttgtccgag gtattcaaca gataagcctg gcctttggga aaagtgtgat cggatgcaat
     3241 gccgcgatcg atcagatcct ttacttgcga gaaagaattt ccggccagca tcatcgccgg
     3301 gcgcacctgg tagtcatcaa aaggatgcgg gccggatgcg ttgaaataag ggctgggtgc
     3361 cgttggttcg catttatcgg aacagtattt tttgtcgaag ccgaatgcca gcgcggaagt
     3421 cagcgacatg cagtcgaccc ggtacggcgc tgtccaggca acagcgaagg cttgcacgtg
     3481 ctcaggtgtg agccggtcaa ttttagcctt aagctttatg aactcattag agctcatggt
     3541 actgcgattg ggggggaagt tcaggtggat gacattggtt ttaggtattt gccgcgcacg
     3601 ctgatagtaa tcgcctatct gccgggacag agggtcgtcg tcattgacga tgacggcgat
     3661 atcctcgggc at
//
LOCUS       DAOQKQ010000212         3665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_15503_length_3665_cov_5.818006, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000212 DAOQKQ010000000
VERSION     DAOQKQ010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15503_length_3665_cov_5.818006"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="PKZ49_11360"
     CDS             complement(<1..627)
                     /locus_tag="PKZ49_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HND37203.1"
                     /translation="MASKRLTYFGLLAVFFCGLQGTAQAHNVASVATTDRINDSHEMA
                     SSMNVTLGKSTLLRLPSAISRISVGNPSVADVTLTSANEVYILGKMIGTTNIILWPRN
                     GSTRVVDITVLMDAAALQDKLLEIMPEETDIHVKAAGDSLVLSGTVSDTVKVDRAVAL
                     ADTYVRTSVLGMMMSMQGNVGQGAAGQGGQGGMQGMQILRQGLQPNEDS"
     gene            complement(639..1445)
                     /gene="cpaB"
                     /locus_tag="PKZ49_11365"
     CDS             complement(639..1445)
                     /gene="cpaB"
                     /locus_tag="PKZ49_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HND37204.1"
                     /translation="MRNTGALGMIVVSLLLGSAAVVVAAKWLNNQVNIEASKIIVASQ
                     DINVGTRLTPEILKAIDWPSSSIPEGSFQEAKLLDSRVAKVNLQRGEPVLEAKLAPEG
                     TVGGLSGVIADGKRAITVRVNDVVGVAGFALPGNLVDILVNIKDDNKNPISKIVLEQI
                     LVLAIAQDLGRDETKPKVVNAVTLEVTPEQAERLDLARSVGSLSLVLRNQIDKSIGST
                     DGVRTQDLLGTKIVATEPQKEVEKVVVYKSIAPRPTVEIIRGVQKSSANQ"
     gene            complement(1771..2310)
                     /locus_tag="PKZ49_11370"
     CDS             complement(1771..2310)
                     /locus_tag="PKZ49_11370"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HND37205.1"
                     /translation="MDQLNILVLAGLLLVAVYYDIRSFRIPNKLILAGLITGLLFNVI
                     LPHETGGLSFQSALAGFGVGLILFLPLYLIRAMGAGDIKLMGMIGIFVGPLPILLIAL
                     YTAIAGGILAIIVLLCNGKLNGLVEFFKILLLKFQLSTIAGNSVTISIEQMLPKSTSK
                     LPYGVAIAAGTFFYLMTNF"
     gene            complement(2406..2594)
                     /locus_tag="PKZ49_11375"
     CDS             complement(2406..2594)
                     /locus_tag="PKZ49_11375"
                     /inference="COORDINATES: protein motif:HMM:NF016828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HND37206.1"
                     /translation="MNKLVKSFKQFLNNEEGMGAVEYALIVAVMSVIILAVWGTLQGG
                     LQTAFTNISNTLQNPSVN"
     gene            2899..3447
                     /locus_tag="PKZ49_11380"
     CDS             2899..3447
                     /locus_tag="PKZ49_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HND37207.1"
                     /translation="MKLLFDLFPVILFFIAYKLVDIYAATVAAIIATLIQVGWSWFRH
                     RKVDNLLWVSLVLITIFGGATLFLQDEAFIKWKPTVLYWLFACVLLISQVLFKKNLIR
                     SMLSNQLKLPAAIWSRLNTSWSLFFAAMGAVNLYVAFSFSTDFWVNFKLFGFTGLMLV
                     FIIFQGLFIGKHAEAQDVTKTE"
     gene            complement(3482..>3665)
                     /locus_tag="PKZ49_11385"
     CDS             complement(3482..>3665)
                     /locus_tag="PKZ49_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HND37208.1"
                     /translation="PPAILTAGGRVLCVAVLGENIKLAHQRAYDIAHKIQFSGCQMRH
                     DIGYRAMNRIDPKVDS"
BASE COUNT         1029 a          819 c          710 g         1107 t
ORIGIN      
        1 tgaatcttca ttgggttgca atccctgccg taagatttgc atgccttgca tacccccttg
       61 ccccccctga cctgcagcac cctggccaac attcccttgc atgctcatca tcatgcccag
      121 tactgaagtg cggacatagg tatcggctaa tgctaccgca cgatctacct tgaccgtatc
      181 ggaaaccgtt ccagagagta ctaatgaatc accggccgct ttaacatgaa tatccgtttc
      241 ttctggcatg atctctagta acttatcttg aagtgcagcg gcatccatta gtacggtaat
      301 atccacgacc cgagtcgaac cattgcgcgg ccacaaaata atattcgtgg tgccgatcat
      361 ttttccgaga atataaactt cgttggcact ggttaacgtt acatctgcca cactcggatt
      421 accgaccgat atcctggaaa ttgcggaagg aagtcgcagc aaggttgatt tacccagtgt
      481 cacattcatg ctggaagcca tttcgtgact atcattgatt cgatccgtgg ttgcaaccga
      541 tgccacattg tgtgcttgtg ctgttccttg caatccacaa aaaaataccg ctaataaccc
      601 gaaataagtt aatcgtttag acgccatgat tctccccttt attgatttgc actcgatttt
      661 tgaacacccc gaatgatctc aaccgttggc ctgggggcta tagatttgta cacgacaact
      721 ttctcaactt ctttttgggg ttctgtcgca acaatttttg tacccagcaa atcctgagtg
      781 cgaacaccat cggtagaacc aatactttta tcgatttgat ttcttaacac caaagaaagt
      841 gatccaacac tccttgccag atcgagtctt tccgcttgct ctggagttac ttctagggtt
      901 accgcattga caacttttgg ttttgtttcg tctctcccta aatcctgagc aatcgccaac
      961 actaatatct gctcgagtac gattttagaa attggatttt tgttgtcatc tttaatattt
     1021 accaagatat cgaccaagtt accgggaagc gcaaaaccag ccacgcccac gacatcattc
     1081 acgcgcacgg taatggcacg cttaccatct gcgatcacac cggataatcc ccccaccgtc
     1141 ccttccggtg ctaatttagc ttctagaacc ggttcaccac gttgcaaatt cacttttgcc
     1201 acgcgactat ccaggagttt tgcttcttga aatgagcctt cagggatgct gctgctgggc
     1261 caatcgatgg ctttgagaat ttctggggta aggcgggtgc ctacattaat gtcttgtgac
     1321 gcaacgatta ttttcgatgc ttcgatattc acctgattat taagccactt cgcggcaacg
     1381 accactgccg ctgatcctaa taacaaggag actacgatca ttcctagcgc tcctgtattt
     1441 ctcatcgata gacctcacac ctagttaacg agttatgaat taagtaatgc ctgtaggttt
     1501 attgccatgg ttctcaaggg taactatagt ggttatcgct ctcacgcaat aaatggaaaa
     1561 gatcaataaa ctgaccctat cttgaccgat gcaacgcatc gacatgacat tcggctgcag
     1621 ttgcaataga cagttacttc gactaatgaa tgaacgagtt gaagtcaaat aagtgcacga
     1681 ttatttgact attctctgaa ttgatggtgt tagaaaagaa gaaaattacg agattaaacg
     1741 cggtttatta gtttatctca gattcgccaa ttaaaaattt gtcattaggt aaaagaatgt
     1801 cccggcagca atagctactc cgtaaggtaa tttactagta gacttaggaa gcatttgctc
     1861 aatagaaatt gttaccgaat ttccagctat tgtacttagc tgaaatttaa gcaataaaat
     1921 cttaaaaaat tcgactagtc catttaattt accattgcaa agcaaaacaa tgattgcaag
     1981 tattccacct gctattgcgg tatacagcgc gataagcaat atcggtagtg ggcctacaaa
     2041 aattccaatc atacccatta gtttgatatc acctgcaccc atcgcacgaa tgagataaag
     2101 cggcaaaaaa aggattagtc caactccaaa tccagctaac gctgattgaa aacttaatcc
     2161 accggtttcg tgtggcagaa taacgttaaa aagcaatcca gtaatcagtc cagcaagaat
     2221 cagcttgtta ggtattcgaa aactacggat atcataataa actgcaacga gtaataaccc
     2281 tgcaagtact agaatgttca attgatccat atcgatatct taatttagaa atccacccag
     2341 ttatatctgg gtggataatt ttatggaatt acttcttact tactactgag ataaattaaa
     2401 atagtttagt tgacactagg attttgtaat gtatttgaaa tattagtaaa tgcagtttgt
     2461 aacccacctt gtagcgttcc ccaaactgct agaataatca ctgacattac tgccacgatc
     2521 aaagcatact cgactgctcc catcccttct tcgttgttca aaaattgttt gaatgattta
     2581 actaatttgt tcatgtcttt tctcctgttt aatttgttta acttcttcaa tcgttactgc
     2641 atggtgaaat tctgacaaat taccgccgct tcgcctatcg taccctggta catcgtactt
     2701 ccgtacagca acagcttcag atagcgatat aagaaatcaa aaaaagtagt attactaaaa
     2761 atggagggat gcattaatcg cagcaatggc ctgtaagtga actttagtaa cgcatcaggt
     2821 atgatgagta tttttttaaa gctaggccct gttatttcag ccttcgttta tcgcgtaacc
     2881 tcacttacaa cactcaacat gaaattactt ttcgacctat ttccagtcat attatttttt
     2941 atcgcttata agctggttga tatctacgct gcaacggtag cagcgattat tgcgacgctt
     3001 atacaggtgg gttggtcatg gtttcgtcac cgcaaagtgg ataatttgct atgggtgagt
     3061 ttggttctca tcactatttt tgggggtgca acgctattcc ttcaggatga agcttttatc
     3121 aaatggaaac cgacggtgct ctattggtta tttgcttgtg tgctattgat cagccaggtg
     3181 ctttttaaga aaaatctgat ccgttcaatg ctcagcaatc aactcaaatt accagcagcc
     3241 atttggtcgc gtctgaatac cagttggtca ctattttttg ccgcgatggg agcggttaac
     3301 ctttatgttg catttagctt ctcgactgat ttttgggtca atttcaagct ctttggattt
     3361 acagggctaa tgcttgtttt cattattttc caagggttgt ttatcggaaa acatgcggaa
     3421 gcgcaggatg taacaaaaac agaatgagtt gcgcttgggt aatcgattca atgagacgaa
     3481 atcagctgtc gactttagga tcgattcgat tcatcgcgcg ataaccgata tcatggcgca
     3541 tttgacagcc gctaaattga attttgtgag caatgtcata agcacgttga tgcgccaatt
     3601 taatattttc accaagtaca gcaacacata gtactctccc ccctgccgtt aaaattgcag
     3661 gcggg
//
LOCUS       DAOQKQ010000213         3650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_15608_length_3650_cov_5.837552, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000213 DAOQKQ010000000
VERSION     DAOQKQ010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15608_length_3650_cov_5.837552"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PKZ49_11390"
     CDS             complement(<1..215)
                     /locus_tag="PKZ49_11390"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634011.1"
                     /note="part of NADH-ubiquinone oxidoreductase complex I;
                     shuttles electrons from NADH, via FMN and iron-sulfur
                     (Fe-S) centers, to quinones in the respiratory chain; NuoF
                     is part of the soluble NADH dehydrogenase fragment, which
                     represents the electron input part of NADH dehydrogenase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HND37209.1"
                     /translation="MTQHTQVILAGIDFADPDTWRLTNYLKRGGYKALRKIFSEKISP
                     EVVIDEVKKSALRGRGGAGFPTGLKWS"
     gene            complement(212..688)
                     /gene="nuoE"
                     /locus_tag="PKZ49_11395"
     CDS             complement(212..688)
                     /gene="nuoE"
                     /locus_tag="PKZ49_11395"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HND37210.1"
                     /translation="MLSTESLKKIDKEIAKYPADQKQSAVMSALAIAQTEKGWLSSET
                     MDFVANYLDMPPIAVYEVATFYNMYNLKPIGQYKITVCTNLPCALSGGNDAANYLKQK
                     LGIGFNETSADGRFTLKEGECMGACGDAPVLLVNNKRMCSFMSNEQIDRLLEELKK"
     gene            complement(691..1947)
                     /locus_tag="PKZ49_11400"
     CDS             complement(691..1947)
                     /locus_tag="PKZ49_11400"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HND37211.1"
                     /translation="MAEIRNYTMNFGPQHPAAHGVLRLVMELDGEVIIRADPHIGLLH
                     RATEKLAESKTYIQSVPYMDRLDYVSMMVNEHAYVMAIERLLKIEVPLRAQYIRVMFD
                     EITRILNHLLWLGAHALDVGAMTVFLYAFRDREDLMDCYEAVSGARMHAAYYRPGGVY
                     RDLPDTMPQYQATDGVSDIQVIKQRNENRQGSLLDFIEDFTNRFPGYVDEYETLLTDN
                     RIWKQRLVDIGIVSPERAKALGFTGPMLRGSGIEWDLRKKQPYEVYDQVEFDIPVGVN
                     GDCYDRYLVRIEEMRQSNRIMKQCVAWLRENSGSVIVDNYKIAPPPRAAMKQNMEELI
                     HHFKLFTEGIHVPPGEIYAAVEHPKGEFGIYIISDGANKPYRLKIRAPGFAHLAALDE
                     MSRGHMIADLVAIIGTQDIVFGEIDR"
     gene            complement(2005..2616)
                     /locus_tag="PKZ49_11405"
     CDS             complement(2005..2616)
                     /locus_tag="PKZ49_11405"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HND37212.1"
                     /translation="MSMSRAEKLASSIANILSNKLVDQAIHLNELTVVLESQDLLDVA
                     KILRDHPDLAFDTLIDLCGVDYSTYTNADFAGKARGGRRFAVVYHLLSIKHNHRLRVR
                     SFLNDDELPMIDSVIGIWPSANWFEREAFDLYGIVFVNHPDLRRILTDYGFIGNPFRK
                     DFPLSGHVEMRYDPDQKRVIYQPITIEPREITPYIIREEHYGD"
     gene            complement(2628..3098)
                     /locus_tag="PKZ49_11410"
     CDS             complement(2628..3098)
                     /locus_tag="PKZ49_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HND37213.1"
                     /translation="MDSTVDRGFITTSLDSVINWGRTGSMWPMTFGLACCAVEMMQTG
                     ASRYDLDRFGIVFRPSPRQSDVMIVAGTLCNKMAPALRKVYDQMAEPRWVISMGSCAN
                     GGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGILQLQNKIKRTNTIAR"
     gene            complement(3103..3459)
                     /locus_tag="PKZ49_11415"
     CDS             complement(3103..3459)
                     /locus_tag="PKZ49_11415"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HND37214.1"
                     /translation="MLGNYFPILLFIVVGLLIGILSMLAGWFLAPNNPDDEKLSPYEC
                     GFSAFEDARMKFDVRYYLVAILFILFDLEIAFLFPWAVVLEEIGFFGFVAMLVFLGIL
                     TVGFIYEWKKGALEWD"
BASE COUNT         1149 a          818 c          606 g         1077 t
ORIGIN      
        1 aaactccatt tcaatccggt tggaaagcca gcgcctccac gaccccgtaa tgcagatttc
       61 tttacttcat caataacaac ttcaggtgat attttttcac tgaagatttt ccttaatgcc
      121 ttatatccac ctcgtttaag gtagttggtt aatctccaag tatcaggatc tgcaaaatca
      181 attcctgcta ggataacttg tgtatgctga gtcatttttt taattcctct agcagtcgat
      241 caatttgctc atttgacata aaagagcaca tgcgtttgtt attaactaac aatactggcg
      301 cgtcaccaca cgctcccatg cattcacctt cttttaaggt aaatctgccg tctgcggaag
      361 tttcgttaaa accaatacca agcttctgct ttagatagtt tgccgcatca ttgccgcccg
      421 ataaggcaca aggtaagttg gtgcaaaccg taatcttata ttgaccaatt ggtttcaagt
      481 tgtacatatt ataaaatgta gcaacctcat aaaccgctat tggtggcata tctaagtaat
      541 tcgcaacaaa atccatggtt tcactcgaaa gccaaccttt ttcagtttgt gcaattgcaa
      601 gcgcagacat tactgcagat tgcttctgat cagcaggata tttagcaatc tctttatcaa
      661 ttttctttag ggattcagta cttaacatgt ttaccgatca atttcaccaa aaacgatatc
      721 ttgcgtgcca ataattgcca cgaggtctgc aatcatatgc cctcgagaca tttcatctaa
      781 tgctgccaaa tgggcaaaac ccggtgcacg aatttttaac cgataaggct tatttgctcc
      841 atcagagatt atatatatac caaactctcc tttaggatgc tcaactgcag catagatttc
      901 acccggagga acatgaatac cctctgtaaa aagcttaaaa tgatggatta actcttccat
      961 attttgcttc attgcagcac ggggaggagg cgcaatttta taattatcta caattactga
     1021 cccgctattt tcacgaagcc aagcaacgca ctgcttcatg atgcgatttg attgcctcat
     1081 ttcttcaatt cgaacaagat accgatcgta acaatcacca ttcactccta ccggtatatc
     1141 aaattcgact tgatcgtaga cctcataagg ttgtttctta cgcaaatccc attcaatacc
     1201 agaacctcgc aacatcggtc ctgtaaatcc tagagcttta gctctttcag gggaaacaat
     1261 accaatatca actagccgtt gcttccatat tcgattatct gtaagcagcg tctcatactc
     1321 atcaacataa ccaggaaatc gattagtaaa atcttcgata aaatccaaca aagaaccttg
     1381 acgattttcg tttctttgtt tgattacttg tatatcactt acaccatctg ttgcttgata
     1441 ttgtggcatc gtatcaggca aatcacgata aacaccgcct ggacgataat aagcagcgtg
     1501 catccttgca ccagatacgg cttcatagca atccattaaa tcttctctat cgcgaaatgc
     1561 atacagaaag acagtcattg caccaacatc taatgcatgt gcacctaacc acaaaagatg
     1621 gttcaatatc cgcgtaatct catcgaacat cacacgaata tactgggctc gtaatggcac
     1681 ttcaattttg agtaaccgtt caatcgccat tacataggcg tgctcattaa ccatcatgga
     1741 cacgtaatcc aggcgatcca tgtagggaac tgattgaata tacgttttac tttctgccag
     1801 tttttctgtt gcccgatgta ataatccaat atgtggatca gccctgatta ttacttcacc
     1861 atcaagctcc attactaatc gtaatacacc gtgtgcagct gggtgttgtg ggccaaaatt
     1921 catggtgtaa tttcgtattt cagccatagc ttattttatt tttatagtta aaaaaagttg
     1981 catatcgatc aatctaagct atggctaatc accataatgt tcttcacgaa taatataagg
     2041 tgttatttca cgtggctcga ttgtgatggg ttgatatata actcgtttct gatctggatc
     2101 atatcgcatt tcaacatgac cagataacgg gaaatcttta cggaagggat tcccaataaa
     2161 tccataatca gtaagaattc tccgcaaatc agggtggtta acaaaaacta ttccatataa
     2221 atcgaaagct tcgcgctcga accaatttgc tgaaggccaa ataccaataa ccgaatcaat
     2281 cataggtaat tcatcatcgt taagaaaaga tctcactctt aaacgatggt tatgtttaat
     2341 tgatagtaaa tgataaacaa ccgcaaaccg ccgaccccca cgcgcttttc ctgcaaaatc
     2401 agcattggta taagttgaat agtcaacacc acacagatca attagcgtat cgaatgctaa
     2461 gtcaggatgg tcgcgcaaaa tttttgcgac atccaacaaa tcttgcgatt ccagcactac
     2521 ggttaactca tttagatgaa ttgcctgatc tactaattta ttacttagaa tattagcgat
     2581 cgacgacgca agtttttcag cgcgactcat tgacataaaa ttggtattta acgagcgata
     2641 gtattagttc tttttatctt attctgcaat tgaagaatac cataaagcag tgcttctgct
     2701 gttggtggac agcctggaac ataaatatca acgggcacga ttcgatcaca accacgtacg
     2761 actgagtaag aataatggta atatccgccc ccattggcac acgatcccat ggatataacc
     2821 cagcgtggct cagccatttg atcatatact tttcttaaag cgggcgccat tttattgcag
     2881 agcgttcccg ctactatcat aacatccgat tgtcgagggc ttggtctaaa aacaattcca
     2941 aatcgatcaa gatcgtaacg cgaagccccc gtttgcatca tctcaaccgc acaacaagcc
     3001 aatccaaaag tcattggcca catcgaacct gtacgacccc aattgatgac cgaatctaag
     3061 cttgtcgtta taaatccgcg atctacagta ctatccataa atttaatccc actctaaagc
     3121 gccctttttc cattcgtaga taaagcccac ggtaagaatg cccagaaata ccaacatagc
     3181 aacgaatcca aaaaatccga tttcttctaa tacaaccgcc caaggaaaca agaaagcaat
     3241 ctctagatca aatagaatga ataaaattgc gaccaaataa taccgaacat caaacttcat
     3301 cctggcgtct tcaaaagcgg agaatccaca ctcataaggc gataactttt catcatccgg
     3361 attatttggt gctaaaaacc agcctgccaa cattgataaa atacctatca ataatccaac
     3421 aacaataaaa agcaatattg ggaaataatt accaagcatt gtattaatca taaaaattga
     3481 ttttattctt cttatattat tgacctaagg ctagtcaaat aaaccactgc ataagttatg
     3541 agggttattg gaattgtata ccttgttatt ttttagttaa gtttatgtta agtattttta
     3601 gtattttata ccgaatagga tggttataac gcgatttatc taaaaagata
//
LOCUS       DAOQKQ010000214         3650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_15609_length_3650_cov_5.432545, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000214 DAOQKQ010000000
VERSION     DAOQKQ010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15609_length_3650_cov_5.432545"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="PKZ49_11420"
     CDS             complement(<1..712)
                     /locus_tag="PKZ49_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HND37215.1"
                     /translation="MRIGIDLGGTKIEGVVLDRNSTELLRKRIETRQVEGYQAILHKI
                     AELVLLLENEVGKPCSVGIGTPGAISAVTGKMKNSNTACLNGQPLLEDLQALLNRPLR
                     IANDANCFTLSEALNGAGTGYNVVFGVIMGTGVGGGIVFKGQLHSGHQHIGGEWGHNI
                     LESNGPDCYCGQKGCVETFLSGPGLAADFYRNGGNTEITTNGIVALAAQGDSLAEAAM
                     QRFFDRFGRAMATVVNILD"
     gene            821..2092
                     /locus_tag="PKZ49_11425"
     CDS             821..2092
                     /locus_tag="PKZ49_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HND37216.1"
                     /translation="MKFETIAIHGGYSPDPTTKAVAVPIYQTTSYAFDDTQHGADLFD
                     LKVTGNIYTRIMNPTTAVLEQRMAELEGGIGALGLASGQAAITYSILTIAEAGDNIVS
                     VGTLYGGTYNLFAHTLPQYGLTVRFADYRNPENFKAMIDEKTKALYCESIGNPLGNVV
                     DIAALAEIAHERGVPLIVDNTVPTPYLCRPFEYGADIVVHSLTKYLGGHGNSIGGVLI
                     DSGKFPWADHPNRFKRLNTPDVSYHGVVYTEALGPAAYIGRARVVPLRNMGAAISPFN
                     SFLILQGIETLPVRMDRICSNTLVVAQYLKTHPKVSWIKYAGLPEHPEHPLTQKYMSG
                     KASGILTFGVQGGIAGGARFQDALKLITRLVNIGDAKSLACHPASTTHRQLSAEEMQK
                     AGVSEDMVRLSIGLEHIDDLIDDLEQALAAV"
     gene            2320..2871
                     /locus_tag="PKZ49_11430"
     CDS             2320..2871
                     /locus_tag="PKZ49_11430"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HND37217.1"
                     /translation="MKKYIHFPSTFKKAMLGLSILIGSLSNLGHAQDQLGLLPLSSLR
                     VSDAQAYETCPAVNPSIRHADFVVILQRQANGPINTETVEFSTHDGTALSGVNYVPVS
                     GKLTFLPGETVKSVRVIILSSQNTDDSYFFLTLSNPSIQTVLRDFNGTASIAHLDPEN
                     ETCRNRVSLNADPRLPDPPPVPK"
     gene            complement(2931..>3650)
                     /locus_tag="PKZ49_11435"
     CDS             complement(2931..>3650)
                     /locus_tag="PKZ49_11435"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HND37218.1"
                     /translation="IMRYAVLIIGIYHYKNVPPLRTTYDAEDIANLFAEMSLAYPFSS
                     QTLLLDTQATELAIINALDALAGETDENTLVFIYFAGHGVRAAQPGRFWYYLLPFDGK
                     ADDLTQLEGSAISMEKFSNKLGAINALQWVLVLDCCKAGSIAEHLSRSLPKENNRNWA
                     ILAATTGDSNSYALPHRRHSCFTQYLLEGLSGRAIDQSGTVRIMNLIDYIQRNIQQEP
                     ILQQPVLKARLHQNFVLKHCM"
BASE COUNT         1024 a          854 c          754 g         1018 t
ORIGIN      
        1 ggtcaagtat attgacgact gttgccatgg cacggccaaa tcgatcaaaa aaacgttgca
       61 tagcagcttc cgccaagcta tcaccttgtg ctgcaagcgc cacgatccca ttggttgtaa
      121 tttcggtatt accgccatta cgataaaaat ccgctgccag gccagggcct gagagaaacg
      181 tttcgacaca gcctttctgt ccacaatagc aatcaggccc gtttgattcc aaaatattgt
      241 gtccccattc accaccaatg tgctgatgcc ctgagtgcaa ttgccctttg aatacaatac
      301 cgccacccac acccgtgccc atgatgaccc caaataccac attgtagcct gttccagcac
      361 cattgagcgc ttcacttaag gtaaagcaat tcgcatcatt agcaatacgc agcgggcgat
      421 tcaatagtgc ttgtagatct tcaagtaaag gctgcccatt caggcaagcg gtgttggaat
      481 tcttcatctt gccagttaca gccgagatag cgccaggggt acctattcca actgaacagg
      541 gtttaccgac ctcgttctca agcagcaata cgagttcggc tattttgtgc aagattgcct
      601 gatagccttc cacctgacgt gtttcaatcc gtttcctcag taattctgtg ctattgcgat
      661 ccagcacaac cccttctatt tttgttccac ctaaatcaat gccaattcgc atttatttat
      721 ccttaatgaa attaattcaa cgttgctcgt catgttgcta ttgggctttt aatgaactac
      781 tctaaactta ttttactgaa aacagatact gaggtgattg atgaaatttg aaacgattgc
      841 tatccacggg ggttatagcc cagaccccac gaccaaggca gttgctgttc caatttacca
      901 aacaacttcc tatgcttttg atgatacgca gcatggcgct gatttattcg atttaaaggt
      961 tacgggtaac atctatacac ggattatgaa tccaacgact gccgtccttg aacagcgtat
     1021 ggctgagctg gaaggaggta tcggcgctct cggtctggca tccggtcaag cagcgatcac
     1081 ttactcaatt ttgacgattg cagaagcggg agataatatt gtttcagtcg gtacgttata
     1141 tgggggaacc tataatttat ttgcccatac tcttccgcaa tatggcctca ccgtgcgctt
     1201 tgctgactac cgaaacccag agaattttaa agcaatgatc gacgaaaaaa ccaaagcact
     1261 ctattgcgag tctattggta atccactcgg taatgtcgtg gatattgcag cgttagcgga
     1321 aattgctcat gagagaggcg ttccattgat tgtagataat acggtaccga ctccttattt
     1381 atgccgccct tttgagtacg gcgctgatat tgttgtccat tctttgacta aatatttagg
     1441 ggggcatggc aacagtattg gcggtgtgct gattgattca ggaaaatttc cttgggcgga
     1501 tcatccgaat cgattcaaac gtctgaatac ccctgatgta tcctatcatg gcgttgttta
     1561 tacagaagca ttgggaccgg ccgcctatat tggccgtgca cgcgttgttc cattacgcaa
     1621 catgggggct gccatttcac cttttaatag tttcttaata ttacaaggca ttgaaacgct
     1681 ccccgttcgc atggaccgta tctgctcgaa tacactagtc gttgcgcaat atctcaaaac
     1741 acaccccaaa gttagctgga tcaagtatgc gggcttgcct gaacaccctg agcatcctct
     1801 gacacaaaaa tacatgagtg gcaaagcatc aggcatactg acttttggcg ttcaaggggg
     1861 tattgcgggc ggagcgcgct ttcaagacgc gcttaagctc attacccggc tcgtcaatat
     1921 tggtgatgcc aaatcattag cttgccatcc ggctagtacc acgcatcgtc aattgagtgc
     1981 ggaagaaatg caaaaagccg gggtatcgga agatatggta cgcctatcca ttggattaga
     2041 gcatatcgat gatttaatcg atgacttgga gcaagcgctt gctgcggtat gatgcaaacg
     2101 ctaaattctt catctggatg accctttaga ctttatcctt gataaatcag ttttcagcaa
     2161 aacctctctc caagcataac tattgccctg aagtgtgata tttcacacag cgttacgatc
     2221 tcaataaaac tagcatgaaa ctgctcaagc gcgattaagc ttgagtaatc acaatacacg
     2281 atcttgaaat cgtaactata taaagcaagg agctaattta tgaaaaaata tattcatttt
     2341 ccatcgactt tcaaaaaagc gatgcttgga ttgagtattc taataggcag tttatcaaat
     2401 ctaggtcatg cacaagacca acttggctta cttccactct ccagtttacg ggttagtgac
     2461 gcacaagcct atgaaacatg tccagctgta aatccatcga ttcggcatgc cgattttgtg
     2521 gtaattttac agcgccaggc caatgggccc atcaacactg agacagtaga gttttcaacc
     2581 cacgatggga cagcattatc gggtgtaaat tatgtgcctg taagtggaaa attaacgttt
     2641 ctacccggtg aaacagtcaa atctgttcgt gtcataattt tgagcagtca aaatacagac
     2701 gatagttact ttttcttaac gctttccaat ccgagtatcc aaacggtact tcgtgatttt
     2761 aatggaacag ccagtattgc tcatcttgat ccagaaaacg aaacttgtcg aaatcgagtc
     2821 agcttaaatg ctgatccaag gctaccagac ccgcccccag taccaaaata attctaaggc
     2881 aggactggat caattcattt ccatcgaatt gatccagcaa gctaatgcgc ttacatgcaa
     2941 tgtttcaata caaaattttg atgtaagcgt gctttaagca ctggctgttg caaaataggc
     3001 tcctgctgaa tgttacgctg aatataatcg attaaattca tgatacgtac cgtaccactc
     3061 tgatcaatgg ccctaccact tagtccttcc aataaatatt gagtaaaaca actatgtctg
     3121 cggtggggca gtgcatatga attactgtct cctgtggtag cagccaaaat agcccaattt
     3181 cggttatttt cttttggtaa agaccgactc aaatgctctg caatgctacc tgctttgcag
     3241 caatcaagaa ccagaaccca ttgcaaggca ttgattgcgc ccaatttgtt tgaaaatttt
     3301 tccatgctga ttgcgctccc ctctaactga gttagatcgt cagctttccc atcaaacgga
     3361 agcaagtaat accaaaaccg accaggttgg gctgcacgga caccatgccc agcgaaatag
     3421 atgaaaacca aagtgttttc atcagtttcc ccggctaaag cgtccagtgc atttatgatt
     3481 gccaattcag ttgcttgcgt atccaacaat aaagtttggc tggaaaacgg gtacgccaaa
     3541 ctcatctcag cgaataaatt ggcaatatct tctgcatcat atgtagtacg caatggtgga
     3601 acattcttat aatgataaat tccgattatc agaactgcat agcgcataat
//
LOCUS       DAOQKQ010000215         3605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_15954_length_3605_cov_4.138873, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000215 DAOQKQ010000000
VERSION     DAOQKQ010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15954_length_3605_cov_4.138873"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="PKZ49_11440"
     CDS             <1..629
                     /locus_tag="PKZ49_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37219.1"
                     /translation="IIMAVVKLGSSEDVNKTQIGSGENSYYDGPPPKPGYYTGVIKSM
                     KLGKIASGKNQGADRIELLIEINKGEYKGAGIFHNLNLTDQGKAYVNQFLHAVSNGTD
                     ADMVKIRRVFWEKGATVDTDNPDNRGLCAFIGWGPWKIENNLVVGFNTKMGTNRKTGE
                     PRAEIVRFVNKKPADDIAADEDDDDVESISDTNDDDIVDEDSADDPWG"
     gene            880..1041
                     /locus_tag="PKZ49_11445"
     CDS             880..1041
                     /locus_tag="PKZ49_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37220.1"
                     /translation="MKIKNEQWFHRLQGHSIIERNATREDGGITHTIGRFGAVVPIRL
                     TVCSCGAIW"
     gene            1063..1236
                     /locus_tag="PKZ49_11450"
     CDS             1063..1236
                     /locus_tag="PKZ49_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37221.1"
                     /translation="MENEDVQARLEIARLYEVVRHLAFMVNVIAAYTDLPEQAKEELH
                     KQLESMASTGENK"
     gene            1233..1406
                     /locus_tag="PKZ49_11455"
     CDS             1233..1406
                     /locus_tag="PKZ49_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37222.1"
                     /translation="MIHINVQQAYEIQAKVKDKIKDVTISDIILIIEAANRVSRDYET
                     ALNNVHDAAAEGV"
     gene            1407..1583
                     /locus_tag="PKZ49_11460"
     CDS             1407..1583
                     /locus_tag="PKZ49_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37223.1"
                     /translation="MSDEYDHDFDPEQICANCDCAEDECECDADDRDIVDGQFCVICQ
                     EEEDEPIHQEDDDE"
     gene            2097..2225
                     /locus_tag="PKZ49_11465"
     CDS             2097..2225
                     /locus_tag="PKZ49_11465"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HND37224.1"
                     /translation="MRFVSLHTHTTFSTGDGFGTVSSHVERVAELGMSALALTEHG"
     gene            2218..>3605
                     /locus_tag="PKZ49_11470"
     CDS             2218..>3605
                     /locus_tag="PKZ49_11470"
                     /inference="COORDINATES: protein motif:HMM:NF019353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HND37225.1"
                     /translation="MVSNVSSWVELEKEAKKAGIQPIFGIEIYTAPPEARSKHHMTLL
                     AMNQEGLANINRMVSQSYADFYYKPTVTWETLKKYNAGIIALSGCADSQLASVLLGGK
                     LYGEQRLEYSDRQFQHAIRVIRTYQQVFGRRYYIEVQRFSSFERTCILNPALEKLSRI
                     TGALLVATADVHYPHKSDTRMQSILYGAHRNADIQELETNWELGIPLTYPESDSEILK
                     DLINTGLSAENAYEAIENTAAIAERCAGVELPKAPNLSYPVRDSRQHDHAIKEYVRGL
                     ILKGMRIRGISMEQENYVERVKYEFKIVASKPGFLDYFATVADIVQWAKSQGIAVGPG
                     RGSAAASLICYLLQITEIDPMQYPMMFERFIDPMRSDMPDIDIDFEDERRHEIFEYAR
                     AKYGAEYVANIGTFTGYKGKNSLDDIARVYKVPKWAIDNIKNKLIERPDGHARHDKTL
                     VDTFNTYSDIKKL"
BASE COUNT         1120 a          638 c          864 g          983 t
ORIGIN      
        1 gcataatcat ggctgttgta aagctgggaa gttctgagga cgttaataag actcagattg
       61 gtagcggtga aaatagctac tatgatggac cgccaccgaa gccgggttat tacactggcg
      121 ttatcaagag catgaagctt ggtaaaatcg ccagcggaaa gaatcagggt gctgatcgta
      181 ttgagttact tattgaaatc aataagggcg aatataaggg tgcaggtatt ttccacaacc
      241 tgaaccttac cgatcagggc aaggcatatg ttaaccagtt tttgcacgca gtctccaatg
      301 ggactgatgc tgatatggtt aagattcgcc gcgtcttttg ggaaaagggc gcaactgttg
      361 atacggataa tcccgataat cgtgggcttt gcgcgtttat cggctggggt ccgtggaaga
      421 ttgagaacaa tttagttgtc ggttttaata ccaagatggg aactaatcgc aagactggcg
      481 aaccgcgtgc ggagattgtc agatttgtga ataagaaacc cgccgacgat atcgctgccg
      541 acgaggacga tgacgatgtt gaatccatta gtgatactaa tgatgatgat attgtcgatg
      601 aggattcggc agatgaccct tggggttaaa taaataaaca aaaaggctca ggcttattca
      661 gtaattggat aagtctgggc cttttttatt ggcgttttgt gcttgacaat tgtgatgaag
      721 tgtgagagca tttcaatgtc agaaatggag gccgaatatg ttttggcttt atttaattgg
      781 aataggtgta atcagtataa tcaaaacagc attctattct aatccgaaag ttgtggcagg
      841 gctaacatta gtaacaataa ttaatgatat tctagacgta tgaaaataaa aaacgagcaa
      901 tggtttcatc gacttcaagg acacagtatt attgagcgta atgctacccg cgaggatggt
      961 ggaattacgc ataccattgg acgttttggt gcagttgtgc cgattcgttt aacggtatgt
     1021 agctgcggtg caatatggta atccgctaat gaaagatttt taatggaaaa tgaggatgtg
     1081 caagcgcggc tagaaattgc gcgactgtat gaagtagtgc ggcatttagc gttcatggta
     1141 aatgtgatag cagcatatac cgatttacca gaacaagcca aagaagagct tcataagcaa
     1201 ttagaatcaa tggcaagtac aggagagaat aaatgatcca tattaatgtg cagcaagcct
     1261 atgagattca ggctaaggtt aaggataaga tcaaagacgt taccattagc gatattattc
     1321 ttatcattga ggctgccaat cgtgtatcca gggattacga aactgcactg aataacgtgc
     1381 atgatgctgc ggcggaaggt gtttaaatga gcgacgaata cgatcacgat ttcgaccctg
     1441 agcagatttg tgcaaattgc gattgcgcag aagatgaatg cgaatgtgat gcggatgata
     1501 gagatattgt cgatggacaa ttctgcgtaa tctgtcaaga agaagaagat gagccaattc
     1561 atcaagaaga tgacgatgag taatgattaa aaggtgctca gtaaaagatt gtaaagaccc
     1621 ttatgatgca aaagggtttt gcaagatgca ctatgtcaga aacaaaacct atggaacgcc
     1681 agtcccaata aaacaatgct ttggttgcag aaaggagttt ccctggaata aaatcgcatt
     1741 tatgaacagg gcatattgtg acgaatgcga aaatttaatc cgaaaatatt gggattggct
     1801 tccatctagg ttacaatcta tacaaagtca tggagttaca gtaatttggt ttattaacac
     1861 tttaatcaat caggattttt cctgtgccat atgtggactt caaagaaaag accgaaagaa
     1921 atttcgcaat ttatgtattg accatgacca taattgttgc cctggggctt atggttgtga
     1981 aaaatgcgta agaggattgc tctgtgattt ttgtaatctt ttaattggtc gatatgaaaa
     2041 taacccaagg atatttgatt cagtatcctc ttatctaaaa aaggaggtga tgcccaatgc
     2101 gttttgttag tttacacacc catacaactt ttagtacggg ggatggcttt ggaacagttt
     2161 ctagccatgt tgagcgagtc gcagagcttg gaatgtctgc acttgcacta acagaacatg
     2221 gttagcaacg tttcgtcctg ggtagaatta gagaaagaag ctaaaaaggc cgggatacag
     2281 cctatattcg gaatcgaaat ttataccgca ccgccggaag cacgctctaa acatcacatg
     2341 acattattgg caatgaacca agagggttta gccaatatca atagaatggt aagccaaagc
     2401 tatgccgatt tctattataa gccgactgtt acatgggaaa cgctcaaaaa atacaatgcc
     2461 ggaattattg ccctttcagg atgcgccgat tcacaattgg ctagcgtact cttgggtggt
     2521 aagctttacg gtgaacagag actggaatat tcagaccgcc agtttcaaca tgccattagg
     2581 gtcattcgga cataccagca ggtatttggt agacggtatt acattgaagt acagcgattt
     2641 tctagttttg agcgtacttg cattcttaac ccggccctcg aaaaacttag tagaatcacc
     2701 ggagcattat tggttgcaac ggctgatgtt cattatcccc ataaatctga caccaggatg
     2761 cagtcgattc tttatggagc gcaccgcaac gctgatattc aagagcttga aactaactgg
     2821 gaacttggta taccactaac ctatccagaa tccgactccg agattttaaa agacttaatt
     2881 aatacaggtc tttccgctga gaacgcctat gaagctatag aaaatactgc ggctatcgcg
     2941 gaacgctgtg ccggtgtaga attacctaaa gcacctaatc tgagctatcc tgtgcgggac
     3001 agtcggcaac atgatcacgc aattaaagaa tatgtgcgcg gcttgatttt aaagggaatg
     3061 cgtattcgtg gcatttcaat ggaacaagaa aactatgttg aaagagtgaa atatgaattt
     3121 aaaatcgtgg cttcaaagcc cggttttctg gactattttg ctactgttgc tgatattgtt
     3181 caatgggcta aaagccaagg gattgctgta gggccgggta gaggtagtgc tgcggctagt
     3241 ttgatctgct atttattaca gattacagaa attgatccca tgcaataccc aatgatgttt
     3301 gagcgtttca ttgatcctat gcgtagtgat atgcccgata ttgatatcga ctttgaggac
     3361 gagcgtagac atgagatttt tgaatatgct agagctaaat acggagccga atatgttgcg
     3421 aatatcggca catttaccgg gtacaagggc aaaaactcat tggacgatat tgcacgagtt
     3481 tacaaagtac caaagtgggc aattgacaat atcaaaaaca agcttatcga aaggccggac
     3541 ggacacgcaa ggcacgacaa aacgcttgtg gatacgttca atacatattc agatattaaa
     3601 aaact
//
LOCUS       DAOQKQ010000216         3532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_16547_length_3532_cov_5.062123, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000216 DAOQKQ010000000
VERSION     DAOQKQ010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16547_length_3532_cov_5.062123"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PKZ49_11475"
     CDS             complement(<1..222)
                     /locus_tag="PKZ49_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37226.1"
                     /translation="MRTALIFPFWLLLTSTVIFAQQKNSPVLMMDPVTVTATRSDRLM
                     VEIPQAINTIKRDQFTDHQPGVTLDEFGRN"
     gene            complement(376..1116)
                     /gene="rlmB"
                     /locus_tag="PKZ49_11480"
     CDS             complement(376..1116)
                     /gene="rlmB"
                     /locus_tag="PKZ49_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HND37227.1"
                     /translation="MSTARTIFGFHAITSRIRQHPESIQEIFLDAHRNDQRAQNLIAL
                     AKNQGIRLLICDAEKLNKIVGTTHHQGVVASVLAIQNRATIETILDDISDSTLLLLLD
                     GVKDPYNLGACLRIADAFGAQAVVVPKDNAVGLTSTVYKVASGSAESIPLITVTNFAR
                     TIRLLKQQGIWVIGAAVEANDALDHIKLSGPTAWILGSEGEGMRKLTQENCDQLVRIP
                     MIGHVNSLNVSVAAGICLFETLRQRNRS"
     gene            complement(1168..3378)
                     /gene="rnr"
                     /locus_tag="PKZ49_11485"
     CDS             complement(1168..3378)
                     /gene="rnr"
                     /locus_tag="PKZ49_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HND37228.1"
                     /translation="MSIKKKGNKNQLRSLDPYLAREQQRYGSTALPSREFIQKVLEDQ
                     GTPIDGSTLCKLLNITAKETEFFNRRLTAMIREGQIIRNRKGDICVTNKLDLIKGIVQ
                     GHGDGFGFLIVDENGPDLYLSPREMQKVLNGDRVIARKTGKDRRGRLEGEIVEVLERA
                     NNELVGRFHTKQGVSFVTAENKRISQEILIPSNATLEAQSGQVVIVKIIQQPEAYSQP
                     IGHVIEILGNHDSPEMEIEIALRKFDLPYLFSEAIEKISAKFPKKVLKKDLTKRQDLR
                     HLPLVTIDGETARDFDDAVYCQMEAENFRLYVAIADVSHYVKDDDALDIEAFNRGNSV
                     YFPRRVIPMLPEVLSNGLCSLNPGVDRLCMVCEMLIEPHGVCLEYQFYPAVMHSHARF
                     TYNEVAAILDDGDQTQAIQHPELHPQIQSLYKAYKALRKERKKRGAIDFETLETQMIF
                     NEQGKIAQIVPVKRNEAHYLIEECMLAANVCASDFLQRHQQPTLYRIHEGPVPEKLEA
                     LRNFLKEFGLQLGGGDRPGAKHYAKTLLKIKTRPDVQLLQTVMLQSLQQALYQPENLG
                     HFGLAYESYTHFTSPIRRYPDLLVHRAIKAVLNHATYTPQTNWAELGKHCSMTERRAD
                     EATREVETWLKCFYMQDKVGKDFNGVISGVTAFGLFVSLDQIFVEGLVHISELPSDYF
                     HFDNIKHLLIGERTRKIYRLGDKIQVKLARVDLDERKIDFLLSVTKITKQVKKH"
     gene            3447..3531
                     /locus_tag="PKZ49_11490"
     tRNA            3447..3531
                     /locus_tag="PKZ49_11490"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3481..3483,aa:Leu,seq:cag)
BASE COUNT          993 a          779 c          721 g         1039 t
ORIGIN      
        1 gttacgtcca aattcatcta atgtaacgcc gggttgatga tcagtaaatt gatcccgctt
       61 gatcgtgtta atggcttgcg gtatctcaac cattaatcga tcggatcgag ttgctgtaac
      121 agtaaccggg tccatcatca gaaccggtga gtttttttgc tgagcaaaaa taaccgttga
      181 tgtcaaaagc agccaaaatg gaaaaataag ggcagttcgc acaacataaa ttcgtaatga
      241 ttctcaacag ctaattttaa ctagaatcaa aagttttact ctcaggcagt tccgagttga
      301 tctactttga tcggtttatt actgcatgat aaccatcact gacaaaagca gttatttaaa
      361 atacccaatg atcggctagc ttcgattcct ttgacgcaac gtttcaaaca aacaaatacc
      421 cgctgcaacg gagacgttca aactattaac atggccaatc atcgggatgc gaaccaactg
      481 atcgcagttt tcttgcgtca attttctcat gccttcacct tcagaaccta atatccaagc
      541 agttggacca ctcaatttaa tatgatccaa ggcatcgttg gcttcaacgg ctgcaccgat
      601 gacccaaatg ccctgttgct tcaataatcg gatagtccgc gcaaaattgg tgaccgtgat
      661 gagcggaatg ctctcagcac tcccactcgc tactttataa acagtagacg ttaatcctac
      721 cgcattatcc ttgggcacga ctacggcttg agcaccaaat gcatcggcaa ttcttaagca
      781 agcaccgaga ttgtagggat ctttaacacc atcgagcaat aataacaatg tgctatcgct
      841 gatatcatcc agaatagttt caatggtcgc ccgattctgg atagcgagaa cgcttgcaac
      901 aactccctgg tgatgggtcg tacccactat tttattcaac ttctctgcat cacagattaa
      961 taatcgaata ccttgatttt ttgcgagtgc gattaaattt tgtgcgcgct gatcgttacg
     1021 atgagcatct aaaaatattt cttgaatgct ctccgggtgc tggcgaatac ggctagtaat
     1081 tgcatggaag ccaaaaatag ttcttgcagt agacatggat tagtcggaga attaaatcga
     1141 ggttaaatag ttgaattgat acaaaaacta gtgtttctta acctgtttcg ttatttttgt
     1201 cacgcttaat aaaaaatcga ttttgcgctc gtcgagatca acccgtgcga gcttgacttg
     1261 tattttatcg cccaatcgat atattttccg ggttcgttcg ccaatcaaca ggtgtttgat
     1321 attatcaaaa tggaagtaat cactgggaag ttctgaaata tgaacaagcc cttctacgaa
     1381 aatttgatca agggagacga acaacccgaa cgccgttaca ccactaatga ccccattaaa
     1441 atctttacca actttgtctt gcatataaaa acatttgagc caagtttcaa cctcccgtgt
     1501 tgcttcatct gccctgcgct cagtcatcga acagtgcttt ccaagttccg cccaatttgt
     1561 ttggggagta taggtcgcgt gattgagtac tgctttaatt gcacgatgta cgagcaaatc
     1621 aggatatctt cgaataggcg aagtgaaatg ggtataggac tcataagcta agccaaaatg
     1681 ccctaaattc tcaggttgat ataaggcttg ttggagggat tgcagcataa ctgtttgcaa
     1741 tagctgaaca tccggtctag ttttgatttt caacagcgtt ttggcgtaat gtttagcgcc
     1801 gggtcgatca cctcctccga gttgtagtcc aaattctttt agaaaattgc gtaatgcttc
     1861 taatttttca ggaactgggc cttcatgaat gcgatacaac gtcggctgtt gatgacgctg
     1921 caaaaaatct gaggcacaaa cattggcagc cagcatacat tcttcgatta aataatgcgc
     1981 ttcatttctt ttgacaggaa cgatttgagc gatcttacct tgctcattga atatcatttg
     2041 ggtttctagg gtttcaaaat caatagcccc acgttttttt cgttctttgc gtaacgcctt
     2101 atatgctttg tagagcgatt ggatctgtgg atgaagctca ggatgttgaa tcgcttgagt
     2161 ttgatcgcca tcgtctaaga tggcagcaac ctcattatag gtaaatctag catgcgagtg
     2221 cataacggct ggataaaatt gatactctaa acacacgcca tgtggctcaa tgagcatttc
     2281 acaaaccata caaagccgat caacccctgg atttagggaa cataaaccat tggaaagtac
     2341 ttctggtaac attggaatga cccgacgtgg aaaataaacc gagttaccgc ggttaaaggc
     2401 ttcgatatcg agtgcatcat catctttaac atagtgactc acatcagcga ttgccacata
     2461 aagtcgaaaa ttctcagctt ccatttgaca ataaaccgcg tcatcgaaat cacgcgccgt
     2521 ttccccgtcg atagtgacta aaggcagatg gcgaaggtct tgacgtttcg tcaaatcttt
     2581 tttaagcact tttttaggaa attttgcaga gattttttca attgcctcac tgaacagata
     2641 aggcaagtca aatttccgca gtgcaatttc aatctccatt tcaggagaat cgtgattccc
     2701 aaggatttca atcacatggc cgatgggctg ggaataagcc tccggttgct gaataatttt
     2761 tacgatgaca acttggcctg attgggcttc taaagttgcg ttggaaggaa tcagaatttc
     2821 ctggctaatt cgtttattct cagccgtcac aaaactgaca ccctgtttgg tgtgaaatcg
     2881 tccaaccagt tcattattgg cacgttccaa tacttcgaca atctcacctt ctaagcggcc
     2941 ccgccgatct ttaccagttt ttcttgcgat gacacgatca ccgttcaaaa ctttctgcat
     3001 ttctcttgga ctcagataca aatcaggtcc attttcatca acgatgagaa aaccaaaccc
     3061 atcgccatga ccttgtacaa tcccttttat taaatcgagc ttattggtaa cgcaaatatc
     3121 acccttgcga ttccgaatga tttgcccttc acggatcatc gcggtgagtc gacgattaaa
     3181 gaattcagtt tctttagctg tgatattaag caatttacat aatgttgaac catcaatcgg
     3241 tgtcccctga tcttcgagta ctttttgaat aaactctcgg ctgggcaaag cggttgaacc
     3301 atagcgctgt tgctcacgcg caagataggg atcaaggcta cgtagctgat ttttattgcc
     3361 ttttttcttg attgacaaaa aagtagtttc ctatagaatc ggctggcctt atgatttgat
     3421 ataaaggcca agtcataaca atcattgccc agatggcgga attggtagac gcgcatggtt
     3481 caggtccatg tgccttcggg tgtggaggtt cgagtcctct tctgggcacc at
//
LOCUS       DAOQKQ010000217         3281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_18814_length_3281_cov_5.444823, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000217 DAOQKQ010000000
VERSION     DAOQKQ010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18814_length_3281_cov_5.444823"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..106
                     /locus_tag="PKZ49_11495"
     CDS             <1..106
                     /locus_tag="PKZ49_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008186715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HND37229.1"
                     /translation="HAKLAEWEAFYNYHRPHGSLGGKTPYERLIEKMR"
     gene            complement(169..654)
                     /locus_tag="PKZ49_11500"
     CDS             complement(169..654)
                     /locus_tag="PKZ49_11500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HND37230.1"
                     /translation="MAFNAYLNCESIKGESTDQNHPDWIEVVSFSHSLNQPTSGTNGT
                     GGRGAGRVDFASFVITKSVDKASVDFNIFCAKGTHIPKVILEICQETGQNICYLRYEL
                     ENVMIQSVSTSGDSSGRPHETIAFVYDKISWAYTLINNDGSAGTTVGPKKWNIEINTV
                     E"
     gene            1105..1320
                     /locus_tag="PKZ49_11505"
     CDS             1105..1320
                     /locus_tag="PKZ49_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37231.1"
                     /translation="MKARQKLLASSIVASLMAISMSAPTFAESNPCAPKAKAQHQHDM
                     KNPCAGKNPCAAKNSCAAKNPCAAKKQ"
     gene            1476..2153
                     /locus_tag="PKZ49_11510"
     CDS             1476..2153
                     /locus_tag="PKZ49_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HND37232.1"
                     /translation="MKSKIQPLRATVISALFGAVIGLPLTLNGCAMATCRPCVPRAGK
                     RNPCNPCAAKNPCAAMNPCAAKNPCAAGDRVDPKAVTRPEGTKPYQGDRAALLNEGKA
                     LWNSKEISRNNSMSCSTCHQDGTAMLQPDFAKPYPHFVQMAADSAGKASITLEEMVQL
                     CMIKPMQSKPFAWDSRQLAALTAYTSVLQKAFKPVGNAANPCAAKNPCAAKNPCAIRN
                     PCAVKPN"
     gene            2205..2948
                     /locus_tag="PKZ49_11515"
     CDS             2205..2948
                     /locus_tag="PKZ49_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37233.1"
                     /translation="MDNGKLEMDENFFSQLSREQLERMAEAGAQIIECYRVLQKAGLN
                     VVGEVLKGQGEFYELEHYPKDDVYDGETHSQYYYHAHRSETNENGHFHTFLRQPGMPA
                     GIFPIPYSGTESWPEGKDALSHLIGISMDAYGFPLGLFATNRWVTAEAWYKSEDVISM
                     LDHFKIDHAYPSWPVNQWISAMLILFRPQIEALLRQRDQVITERARKHQGIDVFEERS
                     LELTGYLPINVEQQIKYTVAVIENHNFKA"
     gene            3033..>3281
                     /locus_tag="PKZ49_11520"
     CDS             3033..>3281
                     /locus_tag="PKZ49_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37234.1"
                     /translation="MNNEHSQPKTSQYPILGVRQVKKTSLLVAGYQKIVALLNNSADQ
                     LPPFLLRLVLAYEFWEAGIMKIESLRVFRRPFSLSQATL"
BASE COUNT          990 a          692 c          687 g          912 t
ORIGIN      
        1 gcatgccaaa ttagccgagt gggaagcttt ctacaactat caccgacctc atggaagttt
       61 gggaggaaaa actccgtatg aacgtttaat cgaaaaaatg agataattat ttcagtctac
      121 cgaagtcgtt tccatcacat tttaatacaa tattagccat catcttaatt actcgacagt
      181 atttatttca atattccatt ttttgggacc taccgttgtc ccagcagaac catcgttatt
      241 tatcagtgta taagcccatg atattttgtc gtaaacgaaa gctattgttt cgtgcggacg
      301 gcccgaacta tctccactag ttgagacaga ttgaatcatc acattctcaa gttcgtatct
      361 aaggtaacaa atgttttgac cagtttcctg gcagatttcg aggattactt tgggaatatg
      421 tgttcctttt gcacagaata tattgaaatc aacactcgct ttatcaacac ttttggtaat
      481 gacgaatgat gcaaaatcaa cacgccccgc tccacgtcct cctgttccat tagtgcctga
      541 agtaggttga tttagactat ggctaaagct aacaacttct atccaatctg gatgattttg
      601 gtctgttgat tcgcctttga tcgattcgca atttagatat gcattaaaag ccacttttac
      661 actccctaat ttagtagttt atgcaacaga atggagtata agcgattaat cgtaaactat
      721 cttgtatctt caattatctc ttacacaaac attgtctatc gaaaattcct gaccaccaac
      781 taactgagaa gtaaacacga aaacacaaat gaggcagaga ttcaaggcga taggtctgta
      841 atcaggagtg gcgacccgca cagaagcaaa agcggagaat ctcccttgga gttctgttaa
      901 taactgtacg gtaaaagcaa tgaaccgaga tcgcacataa agttaatttg ttaaactgaa
      961 taagaggtaa aaatattgtc agggaatcct atttcattcg actaagcatc aggaattagg
     1021 ggaactagtt ccgacttcag gtattaaacc accaaatggt ggttgtttaa tgcataccat
     1081 aatttaatct taggagaact gataatgaaa gctcgtcaaa aacttttggc ttcttccata
     1141 gtagctagct tgatggcaat ttctatgagc gcaccaacat ttgccgagag taatccgtgc
     1201 gcaccaaagg cgaaagcaca acatcagcat gacatgaaaa atccctgtgc tggtaagaat
     1261 ccttgtgcgg ccaagaattc ctgcgctgcg aagaatcctt gcgcagcaaa aaaacaataa
     1321 tctcagcgag ttcaatcccc tgttcttctt aggaaaagct ggttaaaaac cctcagactg
     1381 aagagcgcag ctaatacccc gtgcttctag cgcggggtgc tttaattaat gcacttgtcg
     1441 caacataaga gcttttctgt aacgtaagga aaattatgaa atctaaaatt caaccattaa
     1501 gagcaacagt catttctgct ttatttggag cagtaatcgg gttgcctttg acattgaatg
     1561 gctgtgccat ggcaacttgc aggccatgtg tgccgcgtgc tggtaaacgt aatccgtgta
     1621 atccttgtgc ggcaaaaaat ccctgcgcag ccatgaatcc atgtgccgct aaaaatcctt
     1681 gtgctgcagg tgatagagtg gatcctaagg cggtaactcg acccgaggga acaaaacctt
     1741 atcaaggaga ccgggcggct ttattaaatg aaggcaaagc gttatggaat agtaaagaaa
     1801 ttagtcgcaa caattcgatg tcgtgttcaa cctgtcatca agatggcact gcaatgttgc
     1861 aaccagattt tgctaaacct tatccacact ttgtgcaaat ggccgctgat agcgctggta
     1921 aagcatcaat cacactagaa gaaatggttc aactttgtat gatcaaaccc atgcaaagta
     1981 aacccttcgc atgggattcc cggcaacttg cagcgttgac ggcttataca tcggtattgc
     2041 aaaaagcatt taaaccggtt ggtaatgcag ctaatccttg tgcagccaag aatccatgtg
     2101 cagcaaaaaa cccttgtgct ataagaaatc catgtgctgt taaaccaaat taattactga
     2161 gtcaagggga gggatgaagc gctgacttag gcttcatcgt ttttatggat aacggtaaac
     2221 tggaaatgga cgaaaatttt ttttcacaat tatcccgtga acagctcgag cgaatggccg
     2281 aagctggcgc acagattatc gaatgctatc gggtgctaca gaaagctgga cttaacgttg
     2341 ttggagaagt tctaaaaggg caaggtgagt tttacgaatt agaacactat ccaaaagacg
     2401 atgtgtacga tggcgaaaca cacagtcagt attactatca tgcgcaccgc tcggaaacca
     2461 atgaaaacgg acattttcat actttcttgc gtcaacctgg aatgcccgcc gggatttttc
     2521 ccattcctta cagcggtaca gagtcctggc cggaaggtaa agatgcactt tcccacctta
     2581 tcggcatttc aatggatgct tacggttttc ctctcggatt gtttgccacc aatcgatggg
     2641 taacggcgga agcgtggtat aagtccgaag acgtcattag catgcttgat cattttaaga
     2701 tagatcatgc atatccttcg tggccggtta atcaatggat ttccgccatg ctgattttat
     2761 tccgcccaca gatagaagca ctattgcgac aacgtgacca ggttattact gaacgggcac
     2821 gtaagcatca aggcatagat gtatttgagg aaagaagcct tgagctgact ggttacttac
     2881 cgatcaatgt tgagcagcag attaaataca ctgtggcagt gattgaaaat cacaacttca
     2941 aagcgtgata tttgtttttt tatatcaatc gtatcagtat gtttcttcct tgcgacaact
     3001 aggaatacag cagattacta ctggagcttt acatgaacaa tgaacattct caacccaaaa
     3061 cctcacaata ccccatttta ggcgttcgtc aggtgaaaaa aacttcactg ttagttgctg
     3121 gctatcaaaa aattgtagct ttactgaaca acagcgctga tcagctaccg ccatttctgc
     3181 tacggctagt cctagcttat gaattttggg aggcgggtat tatgaaaatt gaatcgctcc
     3241 gggttttccg gagacctttt agtttgagtc aagccacatt a
//
LOCUS       DAOQKQ010000218         3258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_19085_length_3258_cov_5.890103, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000218 DAOQKQ010000000
VERSION     DAOQKQ010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19085_length_3258_cov_5.890103"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="PKZ49_11525"
     CDS             <1..553
                     /locus_tag="PKZ49_11525"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HND37235.1"
                     /translation="KQASISTRSVKLDLIDPVGSNTINGTSGPDTLQGTTNSDIIFGN
                     GGNDIINGNAGNDRIDGGTGNDRIIGANGSDTLTGGSGQDTFVFNTPISGGIDRISDF
                     SSLDDVIHLARSTFSGLSTGQLPIAAFHIGTGAHDSTDRIIYNSSTGALLYDADGLGG
                     TGSQLFAQLSPGVIVTNTDFYII"
     gene            complement(648..1235)
                     /locus_tag="PKZ49_11530"
     CDS             complement(648..1235)
                     /locus_tag="PKZ49_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HND37236.1"
                     /translation="MLLEKPFNDDEQPINSGINRRFFIKAGLGACIGLVLPQAAYANY
                     TKKTGIRQISFHNLHTGEKVKAVYWEKGRYINGALREIDNVLRDHRTGKRHAIDVRLL
                     DMVQLLHRLLGAKKEFQVVSGYRSPETNRLLAAQRYGVAKHSLHMQGKAIDIRLPEFS
                     LSDVRKAALSMQVGGVGYYPGSNFIHLDSGQVRNW"
     gene            complement(1277..2932)
                     /locus_tag="PKZ49_11535"
     CDS             complement(1277..2932)
                     /locus_tag="PKZ49_11535"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HND37237.1"
                     /translation="MERISSFQQLRFSIAFTYKILLCLISILVLLLVTTGNHFAQANA
                     FVLQEKLAHIPVTTANETSLTMLKRFYVKRDYQPIWWGSSSTSGISIDKVISFIGSAE
                     QHGLDNQDYELNKLIQLQKRDGVDSPSLELELTAIQSLLMLAKDLRSGRLLASKADPD
                     WHIEQHRFDPVDYLNDAIRENHLARSLVELPPQSLHYQLLKTALAKYRDLEVQQQNWQ
                     SIPESAAIRPNTLHKSIPLVRKRILRLYNIQNDPKYGIVKIDNERYDSGLVEAVKQFQ
                     EQHGLNPDGIIGKQTIIALNRTPKQRIQQIRANMERLRWMPRNLGERHVMINIAGFYL
                     SAIEGGKHVLEMRIIVGRDYRSTPSFDSQLSQMVLNPYWNVPQSIAVKDLLPKQQQNS
                     AYFASQGIKVFSRHENVPINPDSINWNSIKGGFPYVLRQNPGKKNALGYIKFLFSNPF
                     SIYLHDTPSKQLFQKDVRTFSSGCIRLEKPWELARFALNGNNSGINLTEKINSGETIT
                     INLTKRVPIYLTYLTAWVDDQDHIHFAEDVYGRDKRLLEYARW"
     gene            complement(3037..>3258)
                     /locus_tag="PKZ49_11540"
     CDS             complement(3037..>3258)
                     /locus_tag="PKZ49_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HND37238.1"
                     /translation="IDEFVHIANRAVTQNERHLYMVQAMQALLERRLQAHGCKLRPDA
                     IFDLLKVDIKLNTLGLETWLDQAETSNKS"
BASE COUNT          966 a          704 c          607 g          981 t
ORIGIN      
        1 gaaacaggct tcgattagca ctaggagtgt taaactcgat ctcatcgatc ccgttggcag
       61 caacaccatc aatggaacat ctggtccgga tacattacaa ggcactacaa atagcgatat
      121 tatctttggt aatgggggta acgatatcat aaacggtaac gcaggaaatg accggattga
      181 tggaggaact ggtaatgatc ggataatcgg tgcaaatgga tcagatacgc taactggtgg
      241 ttctggacaa gatacttttg tgttcaatac gccgatttcg ggaggaatcg atagaattag
      301 cgacttctcc tcgctagacg acgtaataca tctagcgcgt agtacattct cgggattatc
      361 tactggccaa ttaccgattg ctgcatttca tattggaacg ggcgcacatg atagtacaga
      421 tcgtataatt tacaattcat caacaggcgc acttctttac gatgcggatg gtctgggtgg
      481 gacaggttct cagctgtttg cacagctttc acccggtgtc atagttacca acactgattt
      541 ttatatcatc taaggcgtaa tccctcagtt taggcaatat attttactaa actgggggaa
      601 aaattgaatt ctatttatga aataactcga caggcctaca ttgatgatta ccaattacga
      661 acttgcccac tatcaagatg aataaaatta gaacctggat aataaccaac accacccacc
      721 tgcattgaca atgccgcctt cctgacatca gaaagcgaga attctggcaa gcggatatcg
      781 attgctttac cttgcatatg taggctatgt tttgccacac catatcgctg tgcagctaac
      841 aaccggttgg tctcaggcga tcgataacct gaaacaacct gaaactcttt ctttgcaccc
      901 aacaagcggt gcaaaagttg aaccatatca agtaaacgaa catcgatcgc atgccgcttt
      961 ccagtgcgat gatctcttag tacgttatcg atttctctaa gtgcaccgtt aatatatcgt
     1021 cctttttccc agtaaactgc tttaactttc tctccagtat gcaggttatg aaatgaaatc
     1081 tgccttatac ctgtcttttt cgtataattt gcataagctg cttggggcaa caccaatcca
     1141 atacaagccc ctaaacccgc tttaataaag aaccttcgat taataccaga attgattggt
     1201 tgttcatcgt cattaaaagg cttctctaaa agcatcttta accctccagt ctaaaatcaa
     1261 aacttatagc caaaaactac cagcgagcgt actctaatag ccgcttatca cgtccataaa
     1321 catcttcagc aaaatgaata tgatcttggt cgtctaccca agctgttaaa taagttaggt
     1381 aaattggcac acgttttgtt aaattgatgg tgattgtctc accactgtta attttttcag
     1441 ttagattaat tccagaatta tttccattta aagcaaatct tgccagttcc caaggcttct
     1501 ctaagcgaat gcaccctgag ctaaatgttc taacatcttt ttgaaacaat tgctttgaag
     1561 gcgtatcatg taaatatatg ctaaaaggat tcgaaaataa gaactttata tatcctaatg
     1621 catttttctt gccaggattt tgtcttaaaa catagggaaa tccccctttt attgaattcc
     1681 aattaattga atccggatta attggcacat tttcatgtct ggaaaaaact tttattcctt
     1741 gagaagcaaa gtatgcagaa ttttgctgtt gcttgggtag caagtctttt acagcaatac
     1801 tctgaggtac attccagtag ggattgagta ccatttgcga tagctggcta tcgaaacttg
     1861 gagtcgatcg ataatctcga cctacaataa ttcgcatttc caatacgtgc ttgcctcctt
     1921 caattgctga caaataaaac cctgcaatgt tgatcatcac atgccgctca cctaagtttc
     1981 ttggcatcca acgcaagcgc tccatattcg ctcgtatttg ttgaatcctt tgcttagggg
     2041 tcctattcaa cgcaatgata gtttgtttcc caatgattcc atcgggattc aagccatgtt
     2101 gctcttgaaa ctgctttact gcttcaacta gcccggaatc ataccgttca ttatcaattt
     2161 tgacaatacc atacttaggg tcattctgaa tattatagag ccgcaggata cgcttgcgaa
     2221 cgagtggtat gctcttatgc aaagtatttg gacgaatagc cgccgattct ggaattgact
     2281 gccaattctg ctgttgaacc tcaagatctc gatattttgc caatgcagtt tttaataatt
     2341 gatagtgcaa gctctgagga ggtagctcaa ctaaggatcg tgctaaatga ttttcgcgaa
     2401 ttgcatcatt gagataatct acaggatcaa atcggtgttg ctcgatatgc cagtctggat
     2461 cggcctttga ggcgagcaaa cgaccacttc tcaaatcctt tgccagcatt aataaagact
     2521 gtatcgctgt taattctaat tcgagtgagg gtgaatcaac gccatctcgc ttttgtaatt
     2581 gaataagctt atttaactca taatcttgat tatccaatcc atgctgttca gcgctaccga
     2641 taaaggaaat cactttatca atactgattc cagacgtgga gcttgatccc caccaaatcg
     2701 gctgataatc tcgttttaca tagaaacgtt tcaacatagt caaactcgtc tcattggctg
     2761 tagtaacggg gatatgcgct aatttttcct gcaatacgaa tgcatttgct tgggcaaaat
     2821 gattacccgt tgtaactagc aataacacca aaatactgat taagcacaac agtatcttat
     2881 aagtaaatgc aatgctaaaa cgtaattgct ggaaacttga tatccgttcc atatcttctg
     2941 gctataaaaa ggtagaatct ttaagattcc aaatcacaac ccaatgcgat cacattgatt
     3001 caaaatcgca cacaactacc gctgatgttg gataaatcaa gacttattcg aagtttcagc
     3061 ctgatcaagc caggtttcta atccaagcgt attgagcttg atgtcaactt tcaaaagatc
     3121 aaatatcgca tcagggcgca atttacaacc atgcgcttgt aagcgtcgct ctagtagcgc
     3181 ttgcatcgct tgcaccatat agagatgtct ttcattttgg gtaactgcac gatttgcaat
     3241 atgcacaaat tcatcaat
//
LOCUS       DAOQKQ010000219         3182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_19921_length_3182_cov_5.312440, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000219 DAOQKQ010000000
VERSION     DAOQKQ010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19921_length_3182_cov_5.312440"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..2452)
                     /locus_tag="PKZ49_11545"
     CDS             complement(149..2452)
                     /locus_tag="PKZ49_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HND37239.1"
                     /translation="MFLSLLLFLFTLIAILVAILVVPFLRRHLISRFVLKFYNKILPP
                     ISQTEREALDAGTVWWEGELFSGKPDWDKLLAYPKPELTPEEKAFLEGPVEELCAMLN
                     EWQITHELQDLPPHVWQFIKDNGFFGLIIPKSYGGYGFSALAHSEVVMKISSRSGTAA
                     VTVMVPNSLGPAELLLHYGTEEQKNYYLPRLAKGIEVPCFALTGPDAGSDAGSIPDFG
                     IVCRSEYNGQSDVLGMKVTWEKRYITLGPVATLLGLAFRLRDPDHLLGGEVDLGITLA
                     LIPTNTPGVNIGRRHFPLNGAFQNGPNSGKDVFIPMEWIIGGQERVGQGWRMLMNSLA
                     AGRSISLPATSTGAAKLAARTSGAYGRVRVQFKTPVGFFEGVEEALARIAGNTYMMDA
                     ARIMTAGAVDLGEKPSVISAIVKYHLTERSRQAINDAMDIHGGKGICMGPSNYLARTY
                     QQIPVGITVEGANILTRTMIIFGQGAIRGHPYVLQEINAAHLADKKKGLIAFDHALAG
                     HVIFSIRNVYRSLLLGIGGKYLKNHPDSALADTIPYYQQLTRFSAAFALLTDIAMITL
                     GGSLKRRERLSARLGDILSMLYLCSATLKKFENDGQPAADLPLLHWSIQDALYRTQQA
                     FDEFLRNFPGHFLIRIWLRWQVFPLGKRCSPPSDELSHRIARLILEPGEVRERLTAGI
                     YVPTDDNEPLTQLEQALSCAINCEPIEARLRAATKEGQLTAKGDGKIAQAVALNLISA
                     EEAALLDKMKILRRRVIMVDDFTPSFD"
     gene            2690..2983
                     /locus_tag="PKZ49_11550"
     CDS             2690..2983
                     /locus_tag="PKZ49_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37240.1"
                     /translation="MLPEDKKAYLQKITDQLTEWEAKIDTLKAKGEHLAEEAKQEFEE
                     QMAEMNEKRAELSAYLDELTDKADDLWEDVKEEAEEKWDQISATVDHFISKFK"
BASE COUNT          915 a          700 c          736 g          831 t
ORIGIN      
        1 ggctatcttt tgtggtgagc gcgttacccg ttcactaaat aaaccatcca gggtttgggc
       61 tgcttcaatg gggatgacat ctttatgcat gtactgactt tcgttcacgc ttgatttcct
      121 tgttattgtt gttattagca gcgatcaatt aatcaaaact cggcgtgaaa tcatcgacca
      181 taatgacccg gcggcgcagg attttcattt tatcgagtaa agcggcttct tcagcgctga
      241 ttaaattgag tgccacggct tgtgcgatct tgccgtcccc tttagcagtc aattgaccct
      301 ctttcgttgc ggcacgtaat cttgcttcaa tcggttcgca attgatcgca caagataatg
      361 cttgttcaag ttgcgttagt ggttcattgt catcggtggg aacataaata cccgctgtta
      421 agcgctcacg aacttcaccc ggttcgagaa tgaggcgagc aatgcgatgc gatagctcgt
      481 ctgatggtgg tgagcagcgt ttgccaagcg ggaaaacttg ccatctgagc caaatccgaa
      541 taagaaaatg gccgggaaaa ttacgtaaga actcatcgaa tgcttgttgg gttcgatata
      601 gcgcatcttg aatcgaccag tgtaacaacg gtaagtcggc ggcaggttga ccgtcatttt
      661 caaatttctt caaagtggct gaacataaat aaagcatgct taagatatcg cctagcctag
      721 ctgacagtcg ttctctgcgt ttgagcgagc cgcccagcgt tatcattgcg atatcagtta
      781 ataatgcaaa agccgctgag aaacgagtta gttgctggta atacgggata gtatcggcta
      841 gcgcactatc cgggtggttt tttagatact tacccccgat accgagcagc aagctgcggt
      901 aaacattccg aatgctgaaa atcacatggc ccgccaacgc atgatcaaaa gcgattaacc
      961 ctttcttttt atctgcgaga tgggctgcat tgatttcttg tagcacataa ggatgaccgc
     1021 ggatagcgcc ttgaccaaag atgatcatag tgcgcgtcaa aatatttgca ccttcaacgg
     1081 taatacccac tgggatttgt tgataggtcc gtgccaggta attgctcggt cccatgcaaa
     1141 tcccttttcc gccatgaata tccatggcgt cgttgatcgc ttgccggctg cgttccgtca
     1201 ggtggtattt gacgattgct gagatcacag aaggtttctc accgagatca accgctccgg
     1261 ccgtcatgat acgagcagca tccatcatat aagtattacc ggcaatacgt gcgagcgctt
     1321 cttctacccc ttcgaagaag ccaaccggcg tcttaaattg tacgcgtact cgtccataag
     1381 ccccgctggt gcgcgcagct aatttagcag caccggtact ggtggcgggt aatgaaatcg
     1441 aacgacctgc tgccaagctg ttcattaaca tgcgccatcc ttgaccgacc cgctcttgac
     1501 caccaatgat ccattccatg ggaatgaaaa catctttacc tgaattcggt ccattttgga
     1561 aagcgccatt caatggaaaa tggcggcgac caatattgac gcctggggta ttcgttggaa
     1621 tcaacgcaag ggtaataccg agatcaactt cacctcctaa caaatgatct ggatcacgta
     1681 atcggaaagc taatccaagc agcgtcgcaa ccgggccgag tgtaatgtag cgtttttccc
     1741 aagttacttt catgccaaga acgtcagatt gaccattata ttcactgcgg caaacgattc
     1801 caaaatcagg aatcgaacca gcatcagaac cagcatccgg tccggtcaac gcaaagcacg
     1861 gaacctcgat tcctttggcg aggcggggta gataatagtt tttctgttcc tcggtgccat
     1921 aatgtaacaa taactcagcg gggcccaatg aattcggtac cataacggtt actgcagccg
     1981 taccactgcg agagctgatt ttcatgacga cttcggagtg ggcaagcgca gagaatccat
     2041 aaccaccgta cgatttaggg atgattagtc caaaaaaacc gttgtctttg atgaattgcc
     2101 atacgtgggg cggtaaatct tgcaattcat gcgtaatttg ccactcgttc agcatggcac
     2161 agagttcttc gacgggacct tcgagaaacg ctttttcctc aggcgttaat tccggtttag
     2221 ggtaagccag caatttatcc caatctggct ttccactaaa taattcccct tcccaccaaa
     2281 cggttccagc gtcgagggct tcgcgttcgg tttgtgaaat gggtggaagg attttgttat
     2341 agaattttag aacaaatcgt gaaatcaaat ggcgtcgcaa aaaaggaaca acgagaatgg
     2401 cgacgagaat tgcaatcagt gtaaacaaaa atagcagaag tgacaggaac atagttttac
     2461 ctgtaaaaaa agtaagtgta atgacactgg atacagtacc gtagaaaatg cttatgtcaa
     2521 gccttgcaaa taagaaatta ccaaaatttt gtaataaacc cttgctactg attaaaatca
     2581 attgagaatt aattgcgctg gggctcacaa aaatttaaca taggttagaa ataatacagc
     2641 tgcaacaacc tgtttttatt tgtgaagctt aacgagaaag gagatatcga tgttacctga
     2701 agataaaaaa gcttacttgc aaaaaattac tgatcagctg acggaatggg aagccaaaat
     2761 cgataccctc aaggcaaaag gtgagcacct tgcagaggaa gcgaaacaag aatttgaaga
     2821 acaaatggcg gaaatgaatg agaagcgggc cgaattatcg gcttatttgg atgagttgac
     2881 ggataaggca gacgatctgt gggaagatgt caaagaagaa gcagaagaga aatgggatca
     2941 aattagcgca actgttgatc attttatttc taagttcaaa taggctacta attttgcagc
     3001 agacgattct aataaatcat ctgttgcact agcagtttgt ttaatgtaca gcagtattct
     3061 tactcaatct attattggag atctatgatg aagctcgttt cctatgtggt tttaattaca
     3121 ctagggttaa ccctagcaac ggtacatgca accgaaaccg ttccagaaaa agtagaagcc
     3181 ac
//
LOCUS       DAOQKQ010000220         3179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_19956_length_3179_cov_3.525288, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000220 DAOQKQ010000000
VERSION     DAOQKQ010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19956_length_3179_cov_3.525288"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /locus_tag="PKZ49_11555"
     CDS             complement(<1..1193)
                     /locus_tag="PKZ49_11555"
                     /inference="COORDINATES: protein motif:HMM:NF020811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HND37241.1"
                     /translation="MSDPVPNFQSSRPYVSTFQEYISHGWVVIPLPEKEKGPPPTGFT
                     GAVPPHKIADNDQMQQWALDPNNTKANIAVRLGNECVIDDVAYEVIGIDVDHYGAKTG
                     GDYLEKLEQKCGKLPDTWISTARHDGISGIRFYLVPPGYQFRGKCSNDIEIIQKRHRY
                     GACFPSWNPSAKEYYAWYEPGADLDGISSSNAIPYVNELAILPEAWVAYLSREKRFAD
                     HGEKYNFDLDISINDLKLWADKTLPLDEGIYDHSGGMCPDMVKKAAYWVEFFDNCTTV
                     HDNLIDAHWNILSFAKEGHYGWQTCCKLMESAAGDATRRLDNRNLKSWQGEVRRSKEG
                     ALRKLKGMYDYEKETGDAIGLLAGIGVCTCPEPVKNDASGWPQFAAKPVDQYEMNDDG
                     NAEHWL"
     gene            complement(1836..2000)
                     /locus_tag="PKZ49_11560"
     CDS             complement(1836..2000)
                     /locus_tag="PKZ49_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37242.1"
                     /translation="MEEPVWARLEVIGKFDKNNLDKINLSIWANALGSIGFTVVDSVR
                     LIDHEDELWG"
     gene            complement(2000..2254)
                     /locus_tag="PKZ49_11565"
     CDS             complement(2000..2254)
                     /locus_tag="PKZ49_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37243.1"
                     /translation="MARIYLPNEGKREYPIRVGLEKGVYNFVTDYAADLGMSLSAATR
                     RLILLGARCESEHGHVRMPQSFDRLEKQKSEIEMILEDEY"
     gene            complement(2255..2458)
                     /locus_tag="PKZ49_11570"
     CDS             complement(2255..2458)
                     /locus_tag="PKZ49_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37244.1"
                     /translation="MASDSAYDRARQCLQHGANEAGTGWALLAIADAINEVGDIGYIR
                     GIKLGDHAQKLAFGIPEPVAEGP"
     gene            complement(2451..>3179)
                     /locus_tag="PKZ49_11575"
     CDS             complement(2451..>3179)
                     /locus_tag="PKZ49_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37245.1"
                     /translation="YSIIRITMTTSVKPVSYYAPIWRDLRIAKPGFRKPVELPTNILP
                     ILAFDPGGHTGWSLLRLNLKAPDFTGDNKCIFTDPNLTLRDMLTNRMSRIHWEHGEID
                     CSEYEHSGKENKGVYDCMSLVDTWPSAVVIIEDFILQKLNKGRDLLSPVRIGSKIEYE
                     LYKQGRTVQWQLPAAAKTTVSDSRLKELQLYDSRGGFEHARDADRHVVLFIRRCLGNT
                     QPAKELRHKAWPHIFNERGDLANG"
BASE COUNT          875 a          827 c          549 g          928 t
ORIGIN      
        1 agccagtgtt ccgcgttgcc atcatcgttc atctcgtact gatctactgg cttagcggcg
       61 aattgaggcc acccactggc atcattcttg acaggctcag ggcaggtaca aaccccaatg
      121 ccagccaata aaccaattgc atccccagtt tctttttcgt aatcgtacat tcctttgagt
      181 ttgcgtaacg caccctcttt ggacctacga acttcacctt gccaagattt taaatttcta
      241 ttatctaatc tacgggtagc atcaccagca gcagattcca ttaacttgca gcatgtttgc
      301 caaccgtaat ggccttcttt tgcaaatgaa agaatattcc aatgtgcatc aataagatta
      361 tcgtgaacag ttgtgcaatt gtcaaagaat tcaacccaat aagcagcttt cttgaccata
      421 tctggacaca taccgccaga atgatcgtaa atgccctcat caagcggaag ggtcttatca
      481 gcccaaagct ttaggtcatt aatagaaata tcaagatcga aattgtattt ctcaccatga
      541 tctgcgaaac gcttttccct gcttaaatat gcaacccaag cctcgggaag aattgctaat
      601 tcattaacat aaggaattgc gtttgaagaa ctgattccat ctaaatctgc acccggctca
      661 taccaagcat agtattcttt agcacttggg ttccagcttg gaaagcacgc accataccta
      721 tgtcgctttt gaataatctc aatgtcatta ctgcacttgc ctctgaactg gtaacccggc
      781 ggaactaaat aaaacctaat gccgctaata ccatcatgcc gtgcagtgct aatccatgta
      841 tcaggtaatt taccgcactt ctgttcaagc ttttctaaat aatccccgcc agtcttggca
      901 ccataatgat ctacgtcgat gccaataact tcataagcaa catcatcaat aacacactca
      961 ttgccaagcc ttaccgcaat attggctttt gtgttatttg ggtcaagtgc ccactgttgc
     1021 atttggtcat tgtctgcaat tttatgtggt gggactgcgc ctgtaaatcc tgtaggtggt
     1081 ggcccttttt ctttttcagg taatggaatg actacccaac catgactaat atattcttga
     1141 aaagtagaaa catatggcct ggaactttga aaattgggca caggatcaga caaaatattc
     1201 tccttagttt tccccggctc aacgcttcac gcggagcggt ctattgacca tacgcccagg
     1261 tcagagccta tgcaagcgtc ggcgtgtcgg acctaccaac cactacaggt tgtggtccga
     1321 ctgcccctgc accacaagac gaacaaatta aacaactaat atccacaccc accggcctaa
     1381 tcccttaaat acataaaacc ggcttgtaat tacactggtg caacttacag ttgtgtaact
     1441 tacatccatg tcatttgtcc tccgcattct ttttgccgct ccaataatgc ccgatacccc
     1501 ttgggcctct ttttaattta gcgttataca aggcttgccg cgctagcttc tgctctgcat
     1561 aacgtttaac cctatcgcta aatgtatact gtttcttttt aaacccgctg cacatacagt
     1621 ctttaccatc actgttaatt gtgctgcatc catgattaaa tcccatagcg tttttagtcg
     1681 gtgatgcaaa ataatgcata gtgctatggt gcccacaggc tttacaatac atactcatca
     1741 tttgcgccac gcccaaatat taaatccaat cgacactaat cctgcaacaa tagcaataca
     1801 attaaataga ataatccagc ctgtgctgct catcatcaac cccataactc atcttcatgg
     1861 tctattagcc gcacactatc aaccacggta aatccaatac tacccaacgc attagcccaa
     1921 atgcttaggt ttatcttatc cagattgttt ttgtcaaact tcccaataac ctctaaccgt
     1981 gcccatacag gttcctccat cagtattcat cttctaatat catttcaatc tcactttttt
     2041 gtttttcaag cctatcaaag ctttgcggca tacgaacatg cccatgctct gattcgcagc
     2101 gtgcgcctaa aagaattaat cttctagtag ctgccgataa actcataccc aaatcagccg
     2161 cgtagtctgt aacaaaatta tagacaccct tctccaaccc aacgcggata ggatattcac
     2221 gcttaccttc attgggtaaa taaatccttg ccatctatgg cccttctgct actggctctg
     2281 gaattccaaa tgctaatttt tgcgcgtgat cgccaagctt aatccctctg atataaccta
     2341 tatctcctac ttcatttata gcgtcggcaa ttgctagtag tgcccaacct gtgccagcct
     2401 cattcgcacc gtgttgtaaa cactgcctag ccctatcgta tgcgctgtca ctagccattg
     2461 gctaaatctc ccctttcatt aaaaatgtga ggccacgctt tatgccgtaa ttctttagca
     2521 ggctgtgtat tccccaagca acggcgaata aataacacaa catgccggtc agcatctctc
     2581 gcatgttcaa aaccacctct agaatcgtaa agttgcaact ctttcaaacg tgaatctgaa
     2641 acagttgttt tggccgcagc aggtaactgc cattgaacag tcctgccctg tttatacaac
     2701 tcatattcaa ttttgctacc gatccgcaca ggactcagta ggtcgcgccc tttattcaat
     2761 ttctgaagaa tgaaatcttc aatgatgact acagcggaag gccaagtgtc tactaaactc
     2821 atgcaatcgt aaacaccctt gttttccttg ccgctatgct catactcaga acaatcaatc
     2881 tcgccatgtt cccaatgaat tcggctcatt ctatttgtaa gcatgtctcg tagcgtgagg
     2941 tttgggtctg taaagatgca tttgttatcg cctgtaaaat ctggcgcttt caaattcagc
     3001 cgcagtaaac tccaacctgt gtgcccacct ggatcaaacg ctaaaatagg taagatgttt
     3061 gtcggcaact ctacgggctt tcggaaaccg ggctttgcaa ttcttaaatc acgccaaatc
     3121 ggtgcgtaat aacttacagg cttaacagat gttgtcattg taatccttat aatagaata
//
LOCUS       DAOQKQ010000221         3177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_19980_length_3177_cov_5.574311, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000221 DAOQKQ010000000
VERSION     DAOQKQ010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19980_length_3177_cov_5.574311"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..547
                     /locus_tag="PKZ49_11580"
     CDS             83..547
                     /locus_tag="PKZ49_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HND37246.1"
                     /translation="MSEIAHANIAQEFLTFRLGNEEYGIEILKVQEIRGYDTITQIAN
                     APEFIKGVVNLRGIIVPIIDMRIKFNLGEATYDRFTVVIILNLSGRVVGIVVDGVSDV
                     IGLDAEQVRPTPEFGSVLNTEYIMGLGTVDERMLILLDIEKLMSSTDMGLHE"
     gene            597..3116
                     /locus_tag="PKZ49_11585"
     CDS             597..3116
                     /locus_tag="PKZ49_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HND37247.1"
                     /translation="MGTIKARMTIMIGVMIILMLCGSVIGLYGGYSANARLETVYLDR
                     TASFNDLTIVLDHIQRSRMDAAIAAQSKDIQEARNRASMTIERDHEINGKWRNYLATA
                     LTPEEKQLSDRFIDAWKYYQSLRDQVLNLAINGNFELATENFVNEDRSKFDPAHQALF
                     KLVQLQKQLTELAAHENQKGFNHLIVINCLIIGLGGAFAVGMGLFIMRSVTNSFGKAK
                     KIVDSVATGRFDTEIQVGQNDEASPLTGSVKYLVERMNEFYSAQMQLKKQHDEGKISY
                     RISAEKFSGAYAEMAKMTNDLVAAHIAVKMQVVKVITQYAQGDLSVDMDRLPGEKAKI
                     TEAMDNVKASMRMINNEINYLVEGAVEGDFSRRGDTTKYQYEFKQMIERLNQLMQVSD
                     SGLDEVTKILELLANGDLSGRVIGNYKGKFSQLKNNTNQTVTQLNEIVSQIKLMVAGA
                     ASGDFSQRGDVTKYQNEFNEMIEGLNQLMQICESGLKDMVRILNALAKGDLTQKMTNQ
                     YEGIFGQLRDDSEQTVTQLTEIVNKIREAVESINTSAREISDGNNDLSQRTEEQSANL
                     QRTASSMEELTSTVKQNAENAKHANELAASASNVAVKGGEVVGEVVHTMSSINNSSKK
                     IADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRALAQRSAAAAKEIK
                     ELISDSVSKVEDGTQLVDQAGKTMDEIVSSVKRVTDIMAQIALASQEQSLGIEQVNDS
                     ITKMDEITQQNASLVEEAADASESMREQAEQLAQAVKVFKLAQGVISNKNAQMTDRRG
                     PNRAKNVERIHLSSAVDNAKTGRYILSDPAKSGTEDDWETF"
BASE COUNT         1029 a          519 c          832 g          797 t
ORIGIN      
        1 aaacaagaaa tggtcaatgc ggcatgaccg gtagagagga agtaaagcca tgcaaccaat
       61 attagcggaa agtaatcagg cgatgtcaga aatagcgcat gccaatatcg cgcaagagtt
      121 tctgaccttt cgtttaggaa atgaagaata tggaatagaa atcttaaaag tacaagagat
      181 tcgaggatac gacacgatca cgcaaatcgc caatgcaccg gaattcatca aaggcgtagt
      241 gaatttacgg ggaattatcg taccgattat tgacatgcgg attaagttta atttgggaga
      301 agcgacttac gatcgattca cggtggtgat cattttgaat ttatcgggtc gagtggttgg
      361 gatagtggta gatggggtat cggatgtgat aggtctggat gcagaacaag taaggccgac
      421 accggaattt ggaagtgtgc tcaatacgga atacatcatg gggctgggaa cggttgatga
      481 gcgcatgctt atccttctcg acatcgaaaa actcatgagt agtaccgata tgggattgca
      541 tgagtaacct gcaatgactg ctagaaatgt ttagtgtatt tggagggaag atatttatgg
      601 gaacgattaa agcgcgtatg actatcatga tcggagtgat gatcatattg atgctgtgcg
      661 gtagtgttat tggattgtat ggaggctata gtgccaacgc tagattagag acagtttacc
      721 ttgatcgaac tgcttctttt aatgatttaa ccatcgtact tgatcatatt cagcgcagtc
      781 gaatggacgc agcgattgca gctcaatcta aagatattca agaagcgaga aatcgcgcct
      841 ctatgaccat agagcgtgat catgaaataa acgggaaatg gcgtaactat ttagcaactg
      901 cgcttacgcc agaagagaag caattatccg accgctttat cgacgcatgg aaatattatc
      961 aatctttacg tgatcaagtt ctaaatttgg caattaatgg caattttgaa ttagcaactg
     1021 agaattttgt gaatgaagat agatcaaaat ttgacccagc acatcaagct ttattcaagt
     1081 tggttcaatt acaaaagcag cttacggagc tagcagccca tgagaatcag aagggtttta
     1141 atcatcttat agtaattaat tgtttgatca ttggtctggg gggagcattt gctgtaggaa
     1201 tgggcctatt cataatgcgc tctgtgacga attcgtttgg caaagcaaag aaaattgtag
     1261 atagcgtcgc aactgggaga ttcgatacgg agattcaagt cggccaaaat gatgaagctt
     1321 caccgctaac aggttcagtt aagtatttag ttgagcgcat gaatgagttt tatagcgctc
     1381 agatgcagct taagaaacag catgatgaag ggaagataag ttatcgaatt tctgcagaaa
     1441 aattctcggg agcttatgcc gagatggcaa aaatgaccaa tgatctggtc gcagcgcata
     1501 ttgccgtgaa aatgcaagtt gttaaggtta ttacgcaata tgcacaaggt gacttgtcag
     1561 ttgatatgga tcgtttgccg ggagagaaag caaaaattac tgaagcgatg gataatgtca
     1621 aagccagtat gcgaatgatt aataatgaaa tcaactacct cgttgagggg gctgttgaag
     1681 gtgatttttc acggcgtggt gatacaacta agtatcagta tgaatttaag cagatgattg
     1741 aacggttgaa tcaattaatg caagtcagtg attcaggtct tgatgaagta acaaaaattc
     1801 tggagctttt agcaaatggt gatctttcgg gcagagtgat tgggaactat aaaggaaaat
     1861 tctcacagtt aaaaaataat actaatcaga cagtgacaca gttaaacgag attgtgtcgc
     1921 agattaaatt gatggttgcc ggagcggcgt ctggggattt tagtcagcga ggagatgtca
     1981 cgaaatatca gaatgaattc aatgagatga tcgaaggatt gaatcagctg atgcagattt
     2041 gcgagtcggg tttaaaggat atggtgagaa tattgaatgc gctagcgaaa ggtgatttaa
     2101 cgcagaagat gacgaaccag tacgaaggga tatttgggca actgcgagat gattcagaac
     2161 agacggtaac acaattgaca gaaatcgtga ataagatcag agaagccgtt gaatcaatta
     2221 atacttcagc gagagagatt tctgacggga ataatgattt atcgcagcgc acggaagagc
     2281 aatcggccaa tttgcagaga acggcatcga gtatggaaga attgacctct acagtgaagc
     2341 aaaatgcgga gaatgccaaa catgcaaacg agctggcggc gtcggcctcc aatgttgcgg
     2401 ttaaaggagg tgaagtagtt ggtgaagttg tgcataccat gagctcgatc aacaacagtt
     2461 cgaagaaaat tgcggacatt atcagtgtga tcgatggcat tgcctttcag actaatattt
     2521 tagcactcaa cgcagcagtc gaggcggcgc gggcaggaga gcagggacgt gggttcgcag
     2581 tagtggcaac ggaagtacgg gcattggcac aacgatcagc ggctgcagct aaggagatta
     2641 aagaattgat tagtgactca gtcagcaagg tcgaagatgg aactcaactg gtggatcagg
     2701 cgggcaaaac aatggatgag attgtatcgt cagtgaaacg tgtgacagac attatggcgc
     2761 aaatcgctct cgcatcacag gagcaaagtt taggtattga acaagtaaat gactcaatca
     2821 cgaagatgga tgagatcacc cagcagaatg catcgctagt agaggaagca gccgatgcat
     2881 cggaatcaat gcgtgagcag gcagaacaac tggcacaggc ggtaaaggta ttcaaattag
     2941 cgcaaggggt gatatctaat aaaaacgcac aaatgactga tcgacgtggg ccaaatcgtg
     3001 caaagaatgt agagcggatt catttgtcta gtgcggtcga taatgctaag acaggacgat
     3061 acatattatc tgatcctgct aaatcaggca ctgaagacga ttgggaaaca ttttagtaat
     3121 acataagttt agagattttc aatagtcgat tgattaattt aactctttga aaaaggt
//
LOCUS       DAOQKQ010000222         3172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_20037_length_3172_cov_5.119346, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000222 DAOQKQ010000000
VERSION     DAOQKQ010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20037_length_3172_cov_5.119346"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="PKZ49_11590"
     CDS             complement(<1..566)
                     /locus_tag="PKZ49_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37248.1"
                     /translation="MMTTTISDHTVRHFREVLFWPVQMMPLAGARNAQPHWELLAQDG
                     KDNPWHRLKDRFQADGLDFDERHYKEFVTFLPYVQRFIYGETRSHISDNASDPPGGSA
                     IKIFRRCGVSALRLILREGEESITLNVEHIELWFFDDINVAFLKVELSATDLPFTWVC
                     DLLYRFGRAYPTGWDEDGHGKHNVHRAE"
     gene            complement(754..1437)
                     /locus_tag="PKZ49_11595"
     CDS             complement(754..1437)
                     /locus_tag="PKZ49_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HND37249.1"
                     /translation="MKTPRILIIEDENQIRRFVRIALETENFDVIEADSGTRGLIEAS
                     TRQPDLVIIDLGLPDLEGKEVIRQIRGWSEMPIVVLSARDREEEKVAALNIGADDYLT
                     KPFGIPELVARVRAHLRRRQSNENQGDPKAHFGDVIVDLAAHTVTRADTEVHLTPIEY
                     RLLLTLINARGRVITHNQLMLAVWGPNYSERSHYLRIYMGHLRQKLERNPAQPEFLLT
                     ELGVGYRLV"
     gene            complement(1434..2816)
                     /locus_tag="PKZ49_11600"
     CDS             complement(1434..2816)
                     /locus_tag="PKZ49_11600"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HND37250.1"
                     /translation="MLLTVVGISIKFGRGPGAWAAALSVMLFDFFFVPPRFSFSVSDV
                     QYLFTFALMLLVALTIGQLAAKLRFEATIANLRERRAQALAVLSKELSGALTVEQVVE
                     IANRNVSGTFNSAVALLLPSSQEKTKVAGTSSSQPTIDTAVAQWVYDHQLPAGLGTHT
                     LSAAASHYLPLKAPMRTRGVLVLEAKDLLFLNEPEEKRLLDTFAAQIALALERVHYVE
                     VAQDALVSMEGERLRNSLLSAISHDLRTPLTALVGLASTLADTNLPENTRKELALAIH
                     NEAQQMTELVTNLLDMARLQADGMKLKKDWQSIEEIVGSACMQLHRVMTTKNLIISIP
                     ANLPLCECDPILIQRVIVNLLDNAAKYTPTGSTISVSASAGNNEMTIMIEDNGPGLPL
                     RQEDKIFEKFARGETESNKPGVGLGLALCKTIIEAHGGKISAENRVDGGARFIVTLPL
                     GIAPIPEDLL"
     gene            complement(2835..>3172)
                     /locus_tag="PKZ49_11605"
     CDS             complement(2835..>3172)
                     /locus_tag="PKZ49_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1595-like element ISNol1 family transposase"
                     /protein_id="HND37251.1"
                     /translation="DCKRLTLQAVILGKVSIESVIYSDGWRGYNGLVDVGYSKHFRVS
                     HGDNEFARDGHCHINGIESFWSFTKRRLAKFNGVSVNFELHLKESEWRWKKQPDALAN
                     ELWQIIRYY"
BASE COUNT          853 a          760 c          714 g          845 t
ORIGIN      
        1 cactcggctc ggtggacatt atgcttgcca tgtccgtctt catcccagcc tgtagggtag
       61 gcgcggccaa aacggtacag caagtcgcat acccaggtga acggtagatc ggtggcggat
      121 aattccacct tgaggaaagc tacgttgata tcgtcgaaaa accagagttc gatatgctcc
      181 acattcagcg tgatggattc ttcgccctcg cgcagaatca gtcgcaaggc ggaaacccca
      241 catcttcgga atattttgat tgccgaacca ccgggcggat cggacgcatt atcggatata
      301 tgactgcggg tttcgccata gataaaacgc tggacatatg gtaaaaaggt aacgaattcc
      361 ttgtagtgtc gttcgtcaaa atcaagccca tctgcttgaa acctgtcctt cagtctgtgc
      421 catgggttgt ctttgccgtc ctgtgccagc aattcccagt ggggttgtgc gtttctggca
      481 cccgcaagcg gcatcatttg cactggccag aaaagcactt cgcggaaatg gcggacagta
      541 tgatctgaaa tagtagtggt catcatgaat ctaatttaca aaagttgtat taatctaact
      601 cggatagcgt caatggaaac ttcaaatcgt tatagaatca ttgaggcttc tcggctaatt
      661 tgcacgggaa gtcaagctac tactattcag gtttctcaat gctttgttta attcatattc
      721 acgctaaact aaaatgattc tgaggctatt gtcttatacc agccggtaac ctaccccaag
      781 ttcagtaagc agaaattcag gctgagctgg attacgctct aacttttgtc gcaaatgccc
      841 catgtaaata cgtagataat gtgatcgctc tgagtaattc ggcccccata ctgccagcat
      901 caattgatta tgcgtaatta cccgcccgcg cgcattgatg agagtcagta gtaaccggta
      961 ttcaatcggt gtgaggtgaa cttctgtgtc agcacgcgtt actgtatgtg cagcaagatc
     1021 aacgatcaca tcaccaaagt gtgcttttgg atcaccttga ttttcgttgg attgacgccg
     1081 acgtaagtga gcccgaacac gcgcaaccag ttccggtata ccgaatggtt ttgtcaaata
     1141 atcatcagcg cctatgttaa gcgctgcgac tttttcttct tcgcgatcac gggcggacaa
     1201 cacgacaatg ggcatttccg accaaccacg gatttgccga atgacttctt ttccttcgag
     1261 atccggtaag ccaagatcaa taatgacaag atcaggttgc ctagtggagg cctcaattaa
     1321 acctcgtgta ccgctatccg cttcgatgac atcaaaattc tctgtttcca gcgctatacg
     1381 tacgaaacgg cgaatctgat tttcatcctc gataatcaga atgcgaggtg ttttcataac
     1441 agatcttctg gtatgggagc gatcccaagt ggtagtgtca caataaagcg ggctccacca
     1501 tcgactcgat tttctgcgga aatttttcca ccatgggctt cgatgattgt tttacacaga
     1561 gccaagccaa gaccaacgcc tggtttgttc gattctgttt ctccacgtgc aaatttttcg
     1621 aatatcttgt cttcttgtcg gagaggtaaa ccaggcccgt tatcttcaat cataatagtc
     1681 atctcattat tcccggcact ggcagaaacc gagatcgtgc tgccagtggg cgtatatttg
     1741 gcagcattat cgagcaaatt gacaataacc cgctgtatta aaatcggatc gcactcacat
     1801 aaaggtaagt tggcaggaat cgaaataatc agattctttg tggtcattac gcgatgaagt
     1861 tgcatgcatg cgctaccaac aatctcttct atcgactgcc aatccttttt cagcttcata
     1921 ccatcggcct gcaatcgcgc catatcgagt aaatttgtga ctaattcagt catttgttgc
     1981 gcctcattat ggatagcaag tgccagttct ttccgggtat tttctgggag attggtgtcg
     2041 gcaagcgtac tggccaatcc cactaaagcg gtcaaagggg tgcgcaaatc atgtgagatc
     2101 gctgaaagta atgaatttcg caaccgttct ccctccattg agaccaatgc atcctgtgca
     2161 acctcgacat agtggacgcg ttccaaagcc agtgcaattt gtgctgcgaa agtgtcgagt
     2221 aaacgttttt cctccggctc gttaagaaaa agcaaatcct tggcttccag aacgagtacc
     2281 cctcgagtgc gcatcggggc tttaagggga aggtaatgtg aagcggctgc agacaatgta
     2341 tgtgtaccaa gaccagcagg caattgatga tcataaaccc actgtgcaac agcggtatcg
     2401 attgtcggtt gagaggatga tgtgccagct actttcgttt tctcttggct actgggcagg
     2461 agcaacgcta ctgctgaatt aaatgttcca ctgacattgc ggtttgcaat ctcaactact
     2521 tgctcaacgg ttaatgcgcc agataactct ttggaaagga ctgccagtgc ttgtgcgcgc
     2581 cgttcgcgta aattggcaat agtcgcttca aatcgtagtt ttgctgcaag ctgtccgatg
     2641 gttaaggcga ctagtagcat caaggcgaaa gtgaatagat attgcacatc ggaaacagag
     2701 aatgagaatc gtggcggtac aaagaaaaaa tcaaacaaca taactgacaa tgctgcggcc
     2761 catgcgccgg gaccccggcc gaatttgatc gaaataccca caacggttaa taacaaggct
     2821 ctagactagc agagttaata atatctgatg atttgccata gttcgttagc aagtgcatca
     2881 ggttgttttt tccaacgcca ttcagattct tttaaatgca gctcaaagtt aaccgacaca
     2941 ccgttaaact ttgccagccg ccgcttggta aaactccaaa acgattcaat accgttaata
     3001 tggcaatgcc catcccgcgc aaattcatta tcaccatggg atacacgaaa atgcttggaa
     3061 tatcctacat ctaccaaacc attataacca cgccagccat cactatagat gacactctca
     3121 atggacactt tacccagtat caccgcttgt aaagtcaagc gcttacagtc cg
//
LOCUS       DAOQKQ010000223         3110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_20745_length_3110_cov_6.286743, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000223 DAOQKQ010000000
VERSION     DAOQKQ010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20745_length_3110_cov_6.286743"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="PKZ49_11610"
     CDS             complement(<1..839)
                     /locus_tag="PKZ49_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HND37252.1"
                     /translation="MLKLPERNRIYQNHHFDSTRWDYFEPRSTDIIVATSYKAGTTWT
                     QAIVAHLLFQDGNFPAPPAEMSPWLDMRIMPLEVILNRLKAQNHRRFIKTHLPLDGMI
                     YHPHFKYLYIARDTRDVFMSLWNHYTGMKEEIFALMNGLPGRVGEELPHPPENIHTFW
                     QNWITRGGFAWENDGWPYWSHLSNVQSWWNFRHLPNIQLFHYSDMLENTEREMRRMAE
                     FLEIEVPEDTWPRIVKAVSFNEMKREGEKYAPGGGHFWKGGAQTFLHKGSNGRWRDVL
                     SEP"
     gene            complement(895..1863)
                     /locus_tag="PKZ49_11615"
     CDS             complement(895..1863)
                     /locus_tag="PKZ49_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HND37253.1"
                     /translation="MQGPEYFTYLSAQHLESLNLSAEAIIQSIEQLLTTARQSHAWNA
                     PKSVIMPDQRYLMATLSAADDPPFLAVKALVLNPCNHARGLADINALVTLLDSDTGVP
                     LAIIDGNWVTAVRTAGLSAVAAKRLAKPDASVIAFIGCGVQARSHLKLFAQLFPLKEL
                     KAFGRGASNRNALCQLGEALGLRSIASESAQAAICDADLIISTVTFSTQLVPFLDARW
                     LKPGAFATVTDLAAPWIKQSFSELDYIVIDDREQEAVMETPLINPHWITGDLTELVCD
                     VIKGRSTDEQKTAFIFRGLALGDLALSALAYQTAQLQQIGFPIKRE"
     gene            complement(1888..2328)
                     /gene="trxC"
                     /locus_tag="PKZ49_11620"
     CDS             complement(1888..2328)
                     /gene="trxC"
                     /locus_tag="PKZ49_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HND37254.1"
                     /translation="MQESIHIVCPHCDAINRIAEHRLSDNPNCGKCHGALFEEQPLEL
                     SAARFHKHIGQNDIPVLVDFWAPWCGPCKMMAPHFAQAAAQLEPHIRLAKVNTEIEQM
                     LGAQYSIRSIPTLVLFSHGQEVARQSGAMGATDIVRWALSHHSG"
     gene            2556..>3110
                     /locus_tag="PKZ49_11625"
     CDS             2556..>3110
                     /locus_tag="PKZ49_11625"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HND37255.1"
                     /translation="MRLIQKALTFDDVLLVPAHSVVLPRDVDLTTHLTRTIKLKLPLV
                     SAAMDTVTEARLAIAIAQEGGIGIIHKNMPINVQAAHVAKVKRFESGIVSEPITIPPN
                     MTVRGVLELIQQHKISGLPVVEDSKVVGIVTNRDLRFETNLDQPIKNIMTPKKRLVTV
                     KEGASREEALALLHKHRLERVLIIN"
BASE COUNT          884 a          735 c          666 g          825 t
ORIGIN      
        1 tctggctccg atagcacatc gcgccagcgg ccattactcc ctttatggag aaaagtttgt
       61 gcgccacctt tccagaaatg cccaccacca ggtgcatatt tctccccttc acgtttcatc
      121 tcattgaagg aaactgcttt cacaatccgt ggccaagtat cctcgggtac ttcaatctca
      181 aggaactcag ccattctgcg catttcacgc tcggtatttt ccaacatatc gctataatga
      241 aataactgaa tattgggtaa atgccgaaaa ttccaccacg actgcacatt ggaaagatga
      301 gaccagtaag gccagccatc gttttcccaa gcaaaaccac cgcgggttat ccaattttgc
      361 cagaaggtat gaatgttctc cggtggatgc ggtaattctt ctccaactct tccgggcaag
      421 ccattcatta gtgcaaaaat ttcttctttc atacccgtgt agtgattcca tagtgacatg
      481 aatacatccc ttgtatcccg ggcgatatag agatatttaa agtgcggatg gtaaatcatt
      541 ccatccaaag gcaagtgtgt tttgataaag cgccgatgat tctgggcttt gagtcgattc
      601 aagataactt cgagtggcat gatacgcata tcgagccagg gggacatctc ggccggtggt
      661 gcaggaaaat tcccatcctg aaaaagtaag tgggccacaa tggcttgcgt ccaggttgtt
      721 cctgctttat aagacgttgc cacaataata tcagtggacc gtggctcaaa ataatcccag
      781 cgcgtactgt cgaaatgatg attttgataa atccggttac gttcaggtaa tttcaacata
      841 gcgctacctt ttttgttagg gttaggaaga gaaaataagt tagcattttt ttaatcactc
      901 gcgtttgatt gggaaaccga tttgctgcag ttgcgcggtt tgataagcaa gtgcagatag
      961 agcaagatcg cctagagcaa gtccacggaa aataaatgct gttttttgct catcggtact
     1021 gcgacctttg atcacatcgc aaacgagttc agttaaatct ccagtgatcc aatgtggatt
     1081 aattaatggt gtttccatca ctgcctcttg ttcacgatca tcaatcacaa tgtaatccaa
     1141 ctctgaaaag ctttgcttga tccaaggcgc agccagatca gtcactgtag caaaggcacc
     1201 cggttttagc catcgcgcat cgagaaaagg gactaattga gtagaaaaag taactgtcga
     1261 gatgatgaga tccgcatcac aaattgctgc ctgtgcactt tcactcgcaa tcgatcttaa
     1321 accaagtgct tcgcctaatt gacatagtgc attgcgattg ctagcgcccc gaccaaatgc
     1381 cttcaactct tttagtggaa atagctgcgc aaacagcttt aaatgactcc gggcttgaac
     1441 accacatcca ataaacgcga taaccgatgc atcaggtttt gctagccgct tggcagcaac
     1501 cgcactcagt ccagcagtac gaacagcggt aacccaattc ccatcgataa tggcaagagg
     1561 cacccctgtg tcgctatcca ataatgttac taacgcattg atatcagcca atcctcgcgc
     1621 atgattgcac ggattgagaa ccaacgcttt aaccgctaga aagggcggat catctgctgc
     1681 agataaggtt gccatcaaat agcgctgatc cggcatgatg acactcttag gtgcattcca
     1741 ggcatgagat tgccgcgctg tggtaagtaa ctgttcgata ctttgaataa ttgcttctgc
     1801 tgaaagattc aggctttcaa ggtgctgggc agataaataa gtaaaatatt caggtccctg
     1861 catcacacaa attcgctagc aagaccttta cccagaatgg tggctaagtg cccaccttac
     1921 aatatcggtc gcccccattg ctcctgactg acgcgctact tcttggccgt ggctaaaaag
     1981 gacgagtgtc ggaatactac ggatactata ttgtgcgccc agcatctgtt cgatttccgt
     2041 attgacttta gccaaacgaa tgtgcggctc caattgcgct gctgcttgcg caaagtgagg
     2101 cgccatcatt ttgcagggcc cacaccaagg tgcccagaaa tcaaccaaca ccggaatatc
     2161 attctgcccg atatgtttat ggaatcgtgc cgcagataat tcaaggggct gttcttcaaa
     2221 taaagcgccg tgacatttgc cacagttagg gttatcactc aagcgatgct cagcaatacg
     2281 attaattgca tcacagtgtg gacaaacaat atggattgat tcctgcatga ttttgttgcg
     2341 actaaataat aagtaatcga aaggatttta gccattatgg catgatttag tcgaattctt
     2401 acttaacaac agatttgtaa caaataaaca ctactgaaat cagaaccaga aaaagtaggc
     2461 attgtaaaaa caaaattcac gaccaaccct cattttcctt cccgataaga aattgatttc
     2521 gttataataa cgctttgtaa cgaatcgtta ttatcatgcg actgattcaa aaagctctga
     2581 ccttcgatga tgtattatta gttcccgcac actcggtcgt attgccacgt gacgttgatt
     2641 taacgacgca tttaactcga acgatcaaac tcaagctgcc gcttgtttcg gctgccatgg
     2701 ataccgtaac ggaagcccgg ctcgccattg ccattgcgca agaaggtggc atcggtatca
     2761 ttcataagaa tatgcccatt aatgtgcagg cagcacatgt tgccaaagta aagcgttttg
     2821 agagtggtat tgtctccgaa ccgattacga ttccaccgaa catgactgtg cgtggtgtgc
     2881 tcgaattgat ccagcagcat aaaatctcag gacttcctgt cgtagaggat tccaaagtag
     2941 tgggtatcgt tacaaaccgc gatcttcgct tcgaaacaaa tctcgaccag cccatcaaaa
     3001 acatcatgac acccaagaag cgtcttgtta ccgtcaaaga aggcgcttca agagaagaag
     3061 cgcttgcgct tttacataaa catcgcttgg aaagagtgtt aattatcaat
//
LOCUS       DAOQKQ010000224         3081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_21086_length_3081_cov_4.106742, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000224 DAOQKQ010000000
VERSION     DAOQKQ010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21086_length_3081_cov_4.106742"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PKZ49_11630"
     CDS             complement(<1..194)
                     /locus_tag="PKZ49_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37256.1"
                     /translation="MFLFSERTGFASVNGVNNTMAQGSRIIATPAERLARISNVSEAH
                     FARSFKEAFGVPPHRYLLTRR"
     gene            481..1179
                     /locus_tag="PKZ49_11635"
     CDS             481..1179
                     /locus_tag="PKZ49_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HND37257.1"
                     /translation="MFIEKHTNDASLNLGFEWNFEQLEKEFPSIRLINLNDRSFLYHQ
                     GDKCSSLFLIKGGIVKLSHLTAQGNELTFALLRKGDIVGCLDIVNADHVMEENAQALG
                     KVDFYRIECNDFKQLITLRSDLSWSVLTSIYARKQQIERKLIATHTQPVKQRVIKMLF
                     ELAQLFGTRCLHGYALEIFLTQQELADLVGASRSVVSTIMNNFRDQGVLNYTREQICI
                     NDSALISIELSSES"
     gene            1276..1635
                     /locus_tag="PKZ49_11640"
     CDS             1276..1635
                     /locus_tag="PKZ49_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37258.1"
                     /translation="MKIISPRSHGYLDFLTVVIFALAPTLLDLSKVPAILSYGLAAAH
                     LVVTLISGFPTAALKLIPFIVQGWIERVVGPALIIAPFALSFSDEILASNFFIAMGVI
                     IIAVGFFTNYQGDNRSY"
     gene            complement(2191..2634)
                     /locus_tag="PKZ49_11645"
     CDS             complement(2191..2634)
                     /locus_tag="PKZ49_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbN"
                     /protein_id="HND37259.1"
                     /translation="MIDLPLDLQERVVCSITAAIKYEIPANILLAIAEKEGGKPGQWV
                     RNSNGTHDVGSMQFNTAYLQDLSKYGITPDHVANTGCYAYDLAAWRVRLHIKNDKGDI
                     WTKAANYHSRTPKYNAKYRADLIIRAVKWADWLQDRSLSSNTHNQ"
     gene            complement(2663..>3081)
                     /locus_tag="PKZ49_11650"
     CDS             complement(2663..>3081)
                     /locus_tag="PKZ49_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbL"
                     /protein_id="HND37260.1"
                     /translation="GVGIQLFVMLLIVGIGSDLLSSFYAKMGKNVLNYEELAVMLIFT
                     IAFFVLISRLPLLLAGIITGSSIGSAVGIGSYSAGGFMAAAGTAAAAASYGGTLAAGR
                     LTAARSAGTDALRNAIEKGQAQESSGMNNISQGRGK"
BASE COUNT          905 a          657 c          594 g          925 t
ORIGIN      
        1 cggcgtgtca gcagatagcg atgtggtggt acaccaaagg cctctttgaa cgaccgtgca
       61 aaatgtgcct cagaaacatt actgatgcgc gccagccgct cagccggcgt cgcaataatt
      121 cgggaacctt gagccatcgt attatttacc ccgttaacag atgcaaaccc agttcgttca
      181 ctaaacagga acatataaag ccaacgtagt ggcgcagtat tacgaattgc agaaactgac
      241 gcaacaataa ctcatatgaa cgtataaaaa cgagaaataa aatttcgccc gcaagcgggc
      301 acctacaata gtttgtattt cactttacac tttacacttt acatataccg acatatatca
      361 attaatgtga cttccgtctt atgcgaagtt ttccaatctg ctactgataa agcgcaaaaa
      421 taacctaccg attattataa tgatcgcatt tctacatacc attgaggagc aaagtaatcg
      481 atgtttattg agaaacacac aaatgatgca tcgctgaatt tagggtttga atggaatttt
      541 gagcaacttg agaaagagtt tccttctatt cggttaatca accttaatga cagatctttt
      601 ctttatcacc aaggagataa atgctcaagt cttttcttga taaaaggtgg tatcgttaaa
      661 ctttctcatc taactgcaca aggaaatgag cttacttttg cgctcctaag aaaaggagat
      721 atagtcggat gtttggatat agtgaatgct gatcatgtta tggaagaaaa cgctcaggca
      781 ctcgggaagg ttgattttta tcgcattgaa tgtaatgact tcaagcaatt aataactttg
      841 cgaagtgatt tatcctggtc agttttaaca tcaatctatg ctcgcaagca acagatcgaa
      901 cgcaaattga ttgctactca tactcagcca gtcaagcaaa gggttatcaa aatgcttttt
      961 gaacttgctc aattatttgg aacacgatgt ctacatggat atgcgctgga aatttttctt
     1021 acccaacaag agctggcaga tctagtaggt gcaagtcgct ctgttgtaag tactattatg
     1081 aataatttta gagatcaggg agtattgaat tatacgagag aacaaatttg tattaatgac
     1141 tcagccttaa tatcaattga gctttcatct gagtcgtgac ttttttcatg gaaggtgttt
     1201 tctaaataac agacttgagt gaagcattct attaaagtac cacatatcat catctttaat
     1261 aaaaggaatg ctctaatgaa aataatttca cctcgtagtc atgggtactt agattttctt
     1321 acagttgtta tattcgcgct agctccaaca ctactggatt tgagcaaggt gcctgctata
     1381 ctgtcttacg gattagcagc agctcatctt gttgtgacac taatttccgg gtttcccact
     1441 gccgctctta agcttatacc atttattgta caaggttgga ttgagcgcgt tgttgggcca
     1501 gcattaatta ttgcaccttt tgccctgagt ttctccgacg agattttagc tagcaacttt
     1561 tttattgcaa tgggagttat tattattgct gtaggatttt tcaccaatta tcaaggtgat
     1621 aaccgctcat actagaatag aaaaaaataa tattttaagt agtaataaga tgttaattat
     1681 taaaaatata atcgtataat cgcttggggc ttattgtaca accgaataat caagagctat
     1741 cgctcctgtt ttttttactt ataagtcaat gcataacgcg cgatcttaca ttgacaacgg
     1801 acagttattt tgaagcatcg ggacgaatga attgaaattc attcgtcatg tattcagctt
     1861 ttgatgcagg cattgacagt tcttacgttc gaaagctggt gcaagcgctg cttcgatctg
     1921 aggctacggc agtcgatttg tcaataccgc caaggggtgg tacagatcaa tcatctggtc
     1981 tagacgagca tggaaaaagt catcagccga catttaagtt tcccctcatt ccctcagatt
     2041 ttgataagaa tagtataaat tctcagggat ttcggcgttt gctaattcgc atgaattcga
     2101 acatttattc ggtttccaag cgttttgcag aaccgactca ttagcgcatt catctacagc
     2161 tttatccaca gattttgtgg aaaagaattc ttactgatta tgggtatttg acgaaaggga
     2221 tcgatcttgc aaccaatccg cccacttcac cgccctgata atcaaatcag cgcgatactt
     2281 tgcattatat tttggagtac gcgaatgata attggctgct tttgtccaga tatcgccttt
     2341 atcatttttg atatgtaacc gcacacgcca tgcggccaga tcataggcat aacaacccgt
     2401 atttgctaca tgatcaggtg tgatgccata tttggataga tcttgaagat atgcggtatt
     2461 aaattgcatt gagccgacat cgtgagtacc gttactgtta cgcacccatt gacccggttt
     2521 accgccctct ttttcggcta tcgccagcaa gatattggcg ggtatttcat acttaatggc
     2581 tgctgtaatt gaacacacca ctctttcttg cagatccaga ggcaaatcta tcatcgagcg
     2641 aatcgcttaa atgccttatt ccttatttac cccttccttg cgaaatattg ttcatacctg
     2701 acgattcctg agcctggcct ttttctatgg cattacgcaa agcatccgtt cctgcactcc
     2761 ttgctgctgt cagccgaccg gctgccagtg tgccgccata agacgcagcg gctgctgccg
     2821 tacctgccgc tgccataaag ccacctgcac tataactgcc aattccgaca gcagaaccta
     2881 tgctactacc cgttataatg ccagccagca acagcggcaa tctggaaatc aatacaaaaa
     2941 atgcaatcgt aaaaatcagc atgaccgcca attcttcata attcagaaca ttcttaccca
     3001 ttttggcgta aaaactggat aacagatcac tgccaatacc aacgatcagg agcatcacaa
     3061 atagctgaat accaacaccc a
//
LOCUS       DAOQKQ010000225         3042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_21554_length_3042_cov_4.752260, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000225 DAOQKQ010000000
VERSION     DAOQKQ010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21554_length_3042_cov_4.752260"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="PKZ49_11655"
     CDS             complement(<1..494)
                     /locus_tag="PKZ49_11655"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111236.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HND37261.1"
                     /translation="MLIFEHSRPGRRNYSQAPITEAEKSNIPKNLLRKSPPLLPEVSE
                     MDTVRHYTRLSQKNFSIDTEFYPLGSCTMKYNPRACNSLAMLPQFLARHPLAPEDTGQ
                     GFMACMYELQEILKDVTGMAGVSLTPMAGAQGELIGVMMIRAYHEARGDTKRTEIIIP
                     DAAHG"
     gene            complement(518..1012)
                     /gene="tpx"
                     /locus_tag="PKZ49_11660"
     CDS             complement(518..1012)
                     /gene="tpx"
                     /locus_tag="PKZ49_11660"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HND37262.1"
                     /translation="MVTLGGKPVKIEGVFPKVGDKAPAFSLVNKDLEDTTLASFAGKK
                     KVLNIVPSLDTPVCALSTRKFNQQASNMSNTVVLIIAADLPFAMKRFCETEGLDKVVV
                     LSTMRGSEFMRNYGVAIAEGPLSGITARAVIVLDENDQIVHAELVPEIKNEPNYAAAL
                     AALN"
     gene            complement(1046..2401)
                     /gene="gcvPA"
                     /locus_tag="PKZ49_11665"
     CDS             complement(1046..2401)
                     /gene="gcvPA"
                     /locus_tag="PKZ49_11665"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HND37263.1"
                     /translation="MPFIPHTEEDIAEMLAKIGANSIEELFDEIPPALKSGKLTRVPA
                     GLTEMEITRLMLERAQADGFYSNFIGAGAYEHHIPAAVWQITTRGEFYSSYTPYQAEA
                     SQGTLQLLYEFQTMMASLTGMDVSNASLYDGASALAEAALMAVRQHKTSRRILIPKTA
                     HPNYRKVLRAIVSNQQIEVVEVPFCEESGQVLVEQIEQFGQEEFAALVIPQPNFFGVL
                     EQVDALTNWAHQKNALVIAVVNPMSLALLTPPGEWGDRGADIAVGEGQPLGIPLSSGG
                     PYFGFMTCKDELVRQMPGRIIGRTVDLDNKPGFVLTLQAREQHIRRSKATSNICTNQG
                     LMVTAATIYMSLLGPDGLRRIAAQSHANTLSLVEKLEKLPGVKKTFSAPFFHEAAITL
                     PGPVAEILQALKAQHILGGLSLDEFYPGQGNTLLVCATETKTKADLDRYVDVLGQILK
                     K"
     gene            complement(2453..2842)
                     /gene="gcvH"
                     /locus_tag="PKZ49_11670"
     CDS             complement(2453..2842)
                     /gene="gcvH"
                     /locus_tag="PKZ49_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HND37264.1"
                     /translation="MSVPSQLKYTTSHEWVKSESDGTVTVGITQHAQELLGDMVFVEL
                     PKVGRKLNQKEECAVVESVKAAADVYSPLAGEVVAANSELESAPEKINQDPYTAWLFK
                     LKLNNAADLEKLLDAKGYQDALDNEAH"
     gene            complement(2874..>3042)
                     /locus_tag="PKZ49_11675"
     CDS             complement(2874..>3042)
                     /locus_tag="PKZ49_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HND37265.1"
                     /translation="ITSGGFSPTLNQSIALARVSSQTAIGETVHVVVRDKQLAARVVK
                     YPFVRNGQALV"
BASE COUNT          826 a          731 c          636 g          849 t
ORIGIN      
        1 ccatgtgcag catcagggat aataatttca gtacgttttg tatcgccacg cgcttcatga
       61 taagcacgga tcatcatcac accgatcaat tcaccttgtg cacctgccat tggcgttaaa
      121 ctcacgcctg ccattccagt aacatctttg agtatttctt gtaactcata catacaagcc
      181 ataaaacctt gaccagtatc ttcaggtgct aatgggtgac gcgcaagaaa ctgcggtaac
      241 atcgcgagcg aattgcaagc tctcgggtta tatttcatcg tacaagagcc caaaggataa
      301 aactcggtat caattgagaa attcttttga gacaaccgag tatagtggcg taccgtgtcc
      361 atttcagaca cctcaggcag caagggaggt gatttgcgca gtaaattttt ggggatatta
      421 cttttttcag cttcagtaat aggcgcttgg gaataattac gccgcccggg gcgtgaatgt
      481 tcaaatatca gcatgataag tttttaagtt gagtaaatta attgagagca gccagtgctg
      541 cagcatagtt aggttcattc ttaatttcag ggactaattc agcatggacg atttgatcgt
      601 tctcgtccaa tacgataaca gctcgcgctg tgatccctga caaaggaccc tccgcgatag
      661 caacaccgta attacgcata aattccgatc cgcgcatggt agataaaaca accactttat
      721 cgagcccttc cgtttcacaa aagcgcttca tcgcaaaagg tagatcagcc gcaataatga
      781 gtaccaccgt attgctcata ttgctcgctt gctggttaaa tttacgcgta gacaatgcac
      841 atactggtgt atccaagctg ggtacaatgt tcaacacttt tttcttaccc gcaaagctag
      901 ctaaagtcgt gtcttcgaga tctttattga ccaatgaaaa cgcaggtgct ttatcgccta
      961 cctttggaaa aacaccttcg atcttgaccg gttttccacc cagtgtaacc attaccgtga
     1021 tgtccttata ttaaaaatta ctgattcatt ttttaagaat ttggcccagt acatcgacgt
     1081 atcgatccaa gtctgctttg gttttggtct cagtagcaca aaccagcagg gtattacctt
     1141 gcccagggta aaattcatcg agactcaatc cacccaagat atgctgcgct ttcagtgctt
     1201 gtaaaatttc agctactggc cccggtaagg taattgctgc ctcatggaaa aaaggtgcgc
     1261 taaatgtttt cttaacacca ggcagtttct caagcttctc aaccagtgat aaggtatttg
     1321 catgcgattg cgctgcgata cgacgcagcc catcgggtcc caatagcgac atataaatcg
     1381 tcgcagccgt caccatcaag ccttggttgg tacaaatatt tgatgttgct ttagaacgcc
     1441 ggatatgttg ttctcgcgct tgcaatgtca gcacgaagcc aggcttatta tccaaatcaa
     1501 cggttcgacc aatgatacga cctggcattt gacgaaccag ctcatcctta cacgtcatga
     1561 aaccgaaata aggcccaccg ctagagagtg gaattcccag tggttgccct tccccaacgg
     1621 caatatcagc accgcgatcg ccccattctc ctggaggcgt caataatgca agcgacatcg
     1681 gatttaccac tgcaatcacc aacgcattct tttgatgcgc ccaattcgtt aacgcatcaa
     1741 cttgctctaa cacgccaaaa aagttgggtt gtggaataac caacgctgca aattcttctt
     1801 ggccaaattg ttcgatttgc tcgaccaaaa cttgccccga ttcttcacaa aaaggaactt
     1861 ctaccacttc gatttgctga ttactgacga tagcgcggag cacttttcgg tagttcggat
     1921 gcgctgtttt gggtattaaa attctacgag acgttttatg ctgacgaaca gccatcaaag
     1981 ccgcttcagc cagcgccgat gcaccatcat aaagactcgc gttcgagaca tccatgcccg
     2041 tcagcgaagc catcatggtc tggaattcgt agagtaattg cagtgttcct tggcttgctt
     2101 ctgcctggta tggcgtataa gaagaataaa attctcctct cgttgtaatt tgccaaactg
     2161 cggctgggat atggtgctca taagccccgg caccaataaa attcgaataa aaaccatcgg
     2221 cctgtgcacg ttcgagcatg agtcgagtga tttccatttc ggtaaggcct gcaggaactc
     2281 gggtgagctt acccgatttc agtgcaggtg gaatttcatc aaatagttct tcaatcgagt
     2341 ttgctccaat cttggcgagc atctcagcga tatcttcttc agtatgcgga ataaagggca
     2401 tgattagttg atcttataaa aaaataaagt cgtagccagt tgaaaatcgc gattaatgcg
     2461 cttcattatc aagcgcgtct tggtagcctt tggcatccag taacttttcc aagtctgcag
     2521 cattgttgag tttgagttta aatagccaag ccgtgtaggg atcttgattg atcttttctg
     2581 gtgcagactc gagttctgaa ttagcagcaa caacctcacc tgctagcggc gaataaacat
     2641 cagccgccgc cttgacggat tcgaccaccg cacattcttc tttttggttt aatttacgtc
     2701 ctaccttagg aagttccaca aataccatat cgccgagtaa ctcctgcgca tgctgagtaa
     2761 taccgacagt cacggtgcca tcgctctcag atttaaccca ttcatgtgaa gttgtatatt
     2821 ttaattgtga aggtacgctc attttttact ccggaaaaaa attaataata aaatcaaact
     2881 aaggcttggc catttcgtac aaacggatat tttacgactc tcgctgcaag ctgcttatct
     2941 ctaacaacca catgcacagt ttcgccgatc gctgtttgac tggaaactcg tgctaatgcg
     3001 atcgattgat tcaatgtcgg tgaaaaaccg ccactggtaa tt
//
LOCUS       DAOQKQ010000226         3029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_21717_length_3029_cov_4.431069, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000226 DAOQKQ010000000
VERSION     DAOQKQ010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21717_length_3029_cov_4.431069"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..343
                     /locus_tag="PKZ49_11680"
     CDS             35..343
                     /locus_tag="PKZ49_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HND37266.1"
                     /translation="MKKISIASTIAALFILTGCGASKDYTPPADATGEQIFSTACTEC
                     HKPLNANVAMILSEKVANKDAIIKKFQGGSMRMPAFKNIQGESADRLAEYILANSEVK
                     "
     gene            608..1285
                     /locus_tag="PKZ49_11685"
     CDS             608..1285
                     /locus_tag="PKZ49_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37267.1"
                     /translation="MASDIKKLEQKLIYAERYMMFFEIITLLLFLSVAVYAGYLIYLH
                     DWEELLNMLPSAAPLCAALLVAKSSTRLIVYYKKIDEIKHAREIIGVTHHSYAILNDL
                     RGRVQHMVSAFTNGNVPLLSIKLNAETIQKRYESLYDRDIYRYLPGDVVNDIGSLSGP
                     IFALVFTSEYFVSTFEKKGLSMLLTLAAKEISEEREQEFSKTLSQLDADLKALTTKFF
                     TIRETLD"
     gene            1501..2811
                     /locus_tag="PKZ49_11690"
     CDS             1501..2811
                     /locus_tag="PKZ49_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HND37268.1"
                     /translation="MTMNRRKFLTTTGLTALAASCAPPTLMSQPLPNVTAHEQLKKLQ
                     HEGLQKQLLLGYPINMKTPPEAFFEWRKQLMAAGLNRFAFNNVGNPYDQSHIPFNSHQ
                     FEKALINRFAAVYGFAQDDVWGFLTNSGTDSNMHGLYMGRTLLKKRTGVLPKIYFTQE
                     AHYSIQILSDVLNLEAVIVKTDMQGNMDVADLESKLADNANQPALIVATIGTTFKGAI
                     DPIDEIQRKLRNRQAYLHLDAALFGGYLPHTPFASDLLQQGMNPEQGVARHHRYDSIA
                     VSCHKFFGFPSPAGLFITTRKIFEEFLQPFSEIHDPEYILQIPGTITCSRDAVKPAEF
                     YFFSSKSAFAQQAKDANTILENARYLYQEMKTHYANLQPTRVSEKSNIVYFRKPSDKI
                     VNKYSLAVMRVNQEGERIPCAHVVVMPHAEKSVLDQFLTDLGKS"
     gene            complement(2814..>3029)
                     /locus_tag="PKZ49_11695"
     CDS             complement(2814..>3029)
                     /locus_tag="PKZ49_11695"
                     /inference="COORDINATES: protein motif:HMM:NF014037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HND37269.1"
                     /translation="PFSRCLTCNGLLAAVPKASIIEQLEPKTRQYYDTFYQCTLCQKI
                     YWEGSHLDHMRHDFAALLSAQPPSQQH"
BASE COUNT          927 a          607 c          663 g          832 t
ORIGIN      
        1 cgaacgagtg aaatcaatca actggagtta gtaaatgaaa aaaataagta ttgcttcaac
       61 catcgcagca ttattcattc tgacgggttg cggcgcgagc aaagactata cgccgcctgc
      121 cgatgcaacc ggagaacaaa ttttcagcac cgcttgcacg gaatgtcata agcctctcaa
      181 cgcaaacgtt gccatgatat tgagcgagaa agttgcgaat aaggacgcga tcatcaagaa
      241 atttcaaggc ggaagcatga gaatgccggc attcaaaaat atccagggag aatctgccga
      301 tcgattggct gaatacattc tagctaatag cgaagtcaaa taatacgaaa ccctgaaaat
      361 aaacaacccc gtggcaagac cacggggaat cgcaaattaa aaccaaaaaa aacaaaggat
      421 cagactcgat agattccgat cgtttgtgag aattgcgttt tgctttctct cgatagagca
      481 attataaaaa ctaaaaggca tgtcgtactt gagttggaat ggctctagtt gttgattgat
      541 agaatcagca gtttctcaga agtgtgttgt atttcatgac ttggccgtgt aggacgcatt
      601 aactgttatg gctagtgata taaaaaaact tgagcaaaaa ctcatttacg ctgaacgtta
      661 catgatgttt tttgaaatca ttaccttact gctgtttttg tctgtagcag tttatgcagg
      721 gtatttgatt tacttacatg attgggagga gctccttaat atgctgccaa gcgccgcacc
      781 gttatgcgcc gcgctgcttg tggcaaagtc atcaacccgt ctaatcgtat actataaaaa
      841 gatagacgaa attaaacacg cacgcgagat tattggcgtt actcatcata gctatgcaat
      901 actaaatgac ttaagggggc gtgtacagca tatggtttct gcctttacca atgggaatgt
      961 accgttatta tccattaagc taaacgctga gacgatacaa aaacgatatg aatcactcta
     1021 tgatagagat atttatcgtt atcttccggg tgatgtagtt aatgacatcg gttccctctc
     1081 cggccctatt tttgctttgg tttttacgag tgagtatttt gtatcaacct tcgaaaagaa
     1141 agggttatca atgctattaa ctcttgcggc aaaagagata agtgaggagc gggaacaaga
     1201 gttttctaaa acactttctc aattagacgc agatctgaaa gcactaacta caaaattctt
     1261 tactattcgt gaaactctgg attagtaata ctgaatattg tacaaactta cgatattggg
     1321 gagttttcac tgacctgtta gactgtttgt agaatcaaga caggtacaaa aaatcatcta
     1381 tagctattta ctcgaaatca acaccgccaa tcgcacaact aatcagcgat ttcagcgtct
     1441 aatggtcatt aatgattcga tatagaccac tatatcgtta accgacgata ggggaatatc
     1501 atgacaatga atcgaagaaa atttttgacc acaaccggac tgacggcact ggcagcatct
     1561 tgcgctccac ccacgctgat gagtcaacct ttacccaatg taaccgcgca tgagcaactc
     1621 aaaaaattac aacatgaagg gttgcaaaag caattactgc taggttaccc cattaatatg
     1681 aagacaccgc ctgaagcgtt ttttgaatgg cgtaagcaac tcatggcggc tggattgaat
     1741 cgctttgcat tcaataatgt gggaaatccg tatgatcaaa gtcacattcc ctttaacagt
     1801 catcaatttg agaaagcgct catcaatcgt ttcgcggcag tctacggttt tgctcaagat
     1861 gatgtttggg ggttcctgac gaatagcggg acggatagca atatgcacgg gctttacatg
     1921 gggcgcacgc tattaaaaaa acgcaccggt gttttgccca aaatttattt tacgcaagaa
     1981 gcgcattatt cgatccaaat ccttagcgat gtgctcaatc ttgaagcagt catcgttaaa
     2041 acggatatgc aaggtaatat ggatgtggct gatttggaaa gtaaacttgc tgacaatgcg
     2101 aatcaacctg cactcatcgt ggctaccatt ggtacgacgt ttaaaggcgc gatcgatccg
     2161 attgacgaaa tacaacgcaa actgaggaat cggcaggctt accttcatct cgatgcggct
     2221 ttatttgggg gttatttacc gcatactcct tttgcgagcg atttgttgca gcaaggtatg
     2281 aaccctgaac aaggggttgc acgacatcat cgctacgatt caattgcggt ttcatgccat
     2341 aaattttttg gatttccgtc tccagcaggt ctgttcatta caacacggaa aatttttgag
     2401 gaatttctgc aaccatttag tgaaattcac gatccagaat atattttaca aattccgggc
     2461 acgattactt gttcgcgtga tgcggtaaaa ccggctgagt tttatttttt tagctcaaaa
     2521 tctgcatttg cccaacaagc aaaggatgcc aacaccatcc tggagaatgc gcgctattta
     2581 tatcaagaga tgaaaacgca ttatgcaaac ttacaaccga ctcgggtgag tgaaaaatcg
     2641 aatattgtct attttagaaa acccagcgat aagatcgtta acaaatattc actggctgtg
     2701 atgcgagtca atcaagaggg agaacgtatt ccttgcgcgc atgtggtcgt gatgccgcat
     2761 gcagaaaaat cggtgcttga tcaatttttg acagacctgg gtaaatcgta atgctaatgt
     2821 tgctgggaag ggggctgagc tgataatagt gcagcaaaat catggcgcat atgatctaaa
     2881 tgcgaacctt cccagtaaat tttctgacaa agcgtgcact gataaaacgt atcgtagtat
     2941 tgtcttgttt tgggttcgag ctgttcaatg atgctcgctt tgggcacggc ggcgagtaat
     3001 ccattgcaag ttaaacaacg ggaaaaggg
//
LOCUS       DAOQKQ010000227         2963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_22575_length_2963_cov_6.736933, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000227 DAOQKQ010000000
VERSION     DAOQKQ010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22575_length_2963_cov_6.736933"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(271..1053)
                     /locus_tag="PKZ49_11700"
     CDS             complement(271..1053)
                     /locus_tag="PKZ49_11700"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HND37270.1"
                     /translation="MAKVAEASRKYRKRYGQLDGFLLWIRLRQHVNAVRGTLHEVRVP
                     GLEFPIFLRAGTSDVLAFIQIFVDGELGFDILTSPRNIVDAGANIGLASIYFAHRFPD
                     AKILALEIDQANFDLLTLNTKAYPNISCTKTALWSGEAQVAIVNPTDEPWAFRVVEVE
                     NGHPKSLRAQGVAGLMQYFKNQHIDLLKIDIEGSEKEVFQNGASEWIDHIGMIAVELH
                     DNIEPGCSLAMSEALSGHRHRLGRSGEYTVVYLDPDGGNAGR"
     gene            complement(1589..2524)
                     /locus_tag="PKZ49_11705"
     CDS             complement(1589..2524)
                     /locus_tag="PKZ49_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37271.1"
                     /translation="MNGLGPHGLNGLFVVLNDSKIIFYDTSDSPTLQNEALEISDAYF
                     KRSYVNDLIPVQYKSRVHPLGLNYELYVGSLNHYEVARFVFRKHLLHDFPKELCVRIA
                     ELLSLSFLPTVVNMAAPPINNQDPQVLFMARAWDPSGEGIELSTEQKVERREINETRA
                     RCIDLLRKEFGSRFYGGFARTAYAMDNYGHLLLGDALVSGKKNYIALLRQYPICIATT
                     GLHGSIGWKMAEYVAFSKAIVSEKLRCEVPGNFDNGKNYFEFSTPEECLQETTKLFEN
                     RELRSKMMQTNWMYYQSHLAPDQLIMRTLNIAGQS"
     gene            complement(2742..>2963)
                     /locus_tag="PKZ49_11710"
     CDS             complement(2742..>2963)
                     /locus_tag="PKZ49_11710"
                     /inference="COORDINATES: protein motif:HMM:NF039953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37272.1"
                     /translation="NCAHSRIGRCAIHLHLVLASYSSGHENVSLNGMCTAAMRAPQAR
                     ILCEGRFVVEQQNIAARGTDCSLAMCEPS"
BASE COUNT          840 a          729 c          618 g          776 t
ORIGIN      
        1 ttggggccag cacccggtga tacatgagca tgacgacctt gccccgatgc cgccaatgaa
       61 acgcacgaat gctataaaag aggccggcca ccgaaattct ggccaggtgt ttcaatctac
      121 tcacaaatta atccatcttg cataatgaat cttacttgct acttccaaag aagtttgatg
      181 acgaacctgc aaaatacggc gacctcgtct gccgcatcat tagctacagt aaaactgctc
      241 agactcaggt gcgatagaag aatcggcata ctagcgcccc gcattaccgc cgtcgggatc
      301 caagtagacc actgtatact cccccgatcg ccctaagcga tggcgatgac ctgaaagggc
      361 ctctgacata gctaggctgc agccgggctc tatattgtcg tgcaattcga cggcaatcat
      421 ccctatatgg tcaatccact cggatgcccc attctgaaat acttcctttt ccgagccttc
      481 tatatcgatt tttagtagat caatgtgttg attcttaaaa tattgcatca gccctgcaac
      541 accttgggcc ctaagcgact taggatgacc attttccacc tcaactaccc tgaatgccca
      601 tggctcatcc gtgggattaa caatagcgac ttgtgcctct ccagaccata gcgccgtttt
      661 cgtgcagctg atattagggt aggcctttgt gttcaaggtg agtaaatcaa agttcgcctg
      721 atcaatttcc aatgccaaaa tctttgcgtc aggaaatcgg tgagcaaaat aaatggaagc
      781 aaggccaata ttagcgccag catcgacaat atttcgcggt gacgtcagga tatcaaaccc
      841 taactcccca tcaacaaaaa tttggatgaa cgctaacacg tctgaagtcc cggctcgcag
      901 gaaaatagga aactccaaac ctggaacacg tacttcgtga agagtgcctc gaactgcatt
      961 gacgtgttgg cggaggcgaa tccacaacaa aaacccatcc aactgtccat atctcttacg
     1021 atatttccgg cttgcttcag caacttttgc caatgtcccc aacataaatc ctcaagcctt
     1081 catttcggat ctgaattaga ttaatggcta tgtatgcttc tgaacttacg tatgcgcaaa
     1141 aaccggccag gagacttgct aactttacaa accagtattc agtctctcat acgcctaatt
     1201 gcgatgagga ttttgaaata gatatgcaaa tgggcatctt agcattaaag attgcccgat
     1261 tcattttggg gatatgatcg ccagaaaatt aacccattca caatgattta tcagtcttgg
     1321 tcaaggcaat acacatggat cacctgccct cctggccaga caacacaagc aacaattgcc
     1381 actcaaaaat aactctatcc gggtttatgc cctggttcat cactagagat gggacggtat
     1441 accgtgatca cttcccaggg gagtggaagt taggatacag aactggattc ctgtaccctt
     1501 gttgagacgt cggacgtaag cacctcttcg cacctctaac cctagctttg gcgtgcagga
     1561 tacggcagct taaaaaaatt ggatccagtc aactctggcc tgcgatgtta agggtcctca
     1621 taatcaattg atccggcgct agatgagatt ggtagtacat ccaattagtc tgcatcattt
     1681 tggatctcag ctcacgattt tcaaatagct tggttgtttc ttggaggcac tcctctggcg
     1741 tagagaattc aaagtagttc ttaccgttat caaaattgcc aggcacctca catcgcaatt
     1801 tctctgaaac tattgccttt gaaaaggcta catattctgc catcttccac ccaatcgaac
     1861 catgcaggcc agtggttgcg atgcaaatgg gatactggcg aagaagagca atatagtttt
     1921 ttttccccga gactaatgcg tcccccaata aaagatgtcc gtagttatcc atggcatatg
     1981 ctgtacgagc gaatccgcca taaaatcgcg agccaaactc tttcctcaac aggtcaatac
     2041 aacgagctcg cgtctcatta atttccctcc gctcaacctt ttgttcagta gataattcaa
     2101 tgccctcacc ggaagggtcc catgctcgcg ccataaataa tacctgggga tcctggttgt
     2161 tgataggtgg tgcggccata tttacgacgg ttggcaggaa actcaaggag agaagctctg
     2221 caatacgcac acacaattct ttagggaaat catgaaggag atgtttacga aatacaaatc
     2281 ttgctacttc gtaatgattc aaactgccaa cgtataactc atagttaagg cctaaaggat
     2341 gcacgcgaga tttatactga accggtatca gatcattgac atagcttctc ttaaagtacg
     2401 catcagaaat ttccagagct tcattctgca atgtgggact gtcacttgtg tcatagaaaa
     2461 taatcttgga atcattcaag accacaaata gaccatttaa tccatgcggc ccaaggccgt
     2521 tcattaatat cctgccacta tgagaatagt ttctgaacgt ttgggttagg cagatttttc
     2581 cagacattgc gaggagcgaa aacccggtat aaatttggga gacatgtggt gaaagactat
     2641 atgcaaatat tttgcatttc attatttaca tgcccattag ttgagggaat ctctcccaaa
     2701 gagtgccctc agtcgtcgga ttgtcaaaat tcaccaaaaa ctcagctagg ctcgcacatt
     2761 gctagggaac aatctgtgcc cctcgctgcg atgttttgct gctctactac gaatcttcct
     2821 tcacataaaa ttcttgcctg aggagcacgc atcgcagcag tacacatacc atttagcgat
     2881 acgttctcgt ggccacttga ataagaggct agaacaaggt gtaaatgaat ggcgcaacgg
     2941 ccgatccttg agtgagcaca att
//
LOCUS       DAOQKQ010000228         2925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_23091_length_2925_cov_5.560976, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000228 DAOQKQ010000000
VERSION     DAOQKQ010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23091_length_2925_cov_5.560976"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /locus_tag="PKZ49_11715"
     CDS             <1..1154
                     /locus_tag="PKZ49_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HND37273.1"
                     /translation="QGTVSLELEAINEEDARQQVLTQGGLVLSVQRHWLDWRPKARVR
                     FPLTHFSQELLSLLTAGLSLVESIETLIEKEEDAGIKKIIENILARLYEGVTFSNAME
                     SHPQAFPALYIATVRASERTGDLPEALTRFITYQNQMDLARKKLVGASIYPVLLLVVG
                     LLVALFLLLFVVPKFSAIYEDIGADLPWLSVLLIKWGRLVHDNGWMLAGIAIASLSLM
                     SYGFSRPAFRAYLMQTLWRIPAIGERMRVYQLARFYKTLGMLLRGGVPITKALEMVSD
                     LLQAHFRPRVRAAASFIREGKTISSAMQTAELTTAVGNRMLRVGERTGLMGEMMERIG
                     NFHDEEISRWVEWATKLIEPILMAVIGTVIGGIIILMYMPIFELAGSIN"
     gene            1166..2848
                     /locus_tag="PKZ49_11720"
     CDS             1166..2848
                     /locus_tag="PKZ49_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HND37274.1"
                     /translation="MHSEAVTKPLLDLAQLTQARQQAMARKITVVKVLEDEGGFSPYD
                     LMQELGRLLHMPVLKMEELHTLQPVYEELTFAEAITQQCVLFQRDGKYLLAVGDPFLP
                     NLRSWVEEYISVIAEWYLVHPADLAAFFAQQEKSMRAMDNVLPAEQQSFVQDGIEDLS
                     LKTIHEGTSHVVRLVHSTLYDAHKSQASDIHLETAPGALAIKYRIDGVLTMIGVMQGT
                     DLAEQVISRIKVMSDLDIAERRVPQDGRFKVSIQGREIDFRVSIMPSIFGEDAVLRIL
                     DRQALADHVEGLTLNQLGFDQSAIATIRRLSAEPYGMLLVTGPTGSGKTTSLYAAISE
                     VNQGDDKIITIEDPIEYQLPGVLQIPVNEKKGLTFARGLRSILRHDPDKIMVGEIRDA
                     ETAQIAIQAALTGHLVFTTVHANNVFDVIGRFSHMGVDPYSFVSALNGIVAQRLVRQL
                     CMHCVVDERPSNEMILESGISLEQAEQYHFRVGRGCGQCRGSGYKGRNAIAEILLLND
                     EIRELIISQATIRRIKEAARQNGTRFLREAALDRVKAGQTSLQEANRVTIVA"
BASE COUNT          805 a          628 c          722 g          770 t
ORIGIN      
        1 atcagggcac ggtatcgctt gagcttgaag caatcaacga agaagatgcg cgccaacaag
       61 tcttgacaca aggtggattg gtattaagcg tacagcgcca ttggctcgat tggcggccta
      121 aagcgcgtgt acgctttccc ctgacgcatt ttagccaaga actattatcg ttgctcactg
      181 ctggactgag tttagtagaa agtatcgaga cattgatcga aaaagaggaa gatgctggca
      241 tcaaaaagat tatcgaaaat atccttgctc gactctatga aggagtaaca ttctcgaatg
      301 cgatggaatc ccatcctcaa gccttcccag cgctatatat cgcaactgta cgggcaagcg
      361 agcgaacggg cgatttaccc gaagccttaa cacggtttat cacgtatcag aatcaaatgg
      421 atctagctcg caaaaagctg gtgggtgcat cgatctaccc cgttttacta ttggttgttg
      481 ggctactggt tgcgctattc ttgctgttgt ttgtcgtccc taaatttagt gcaatttatg
      541 aggacattgg tgcggattta ccttggcttt cagtattact gatcaaatgg ggacgattag
      601 tgcatgacaa tggctggatg cttgcgggta ttgcgatagc cagcttatca ctcatgtcgt
      661 acggtttttc aagacccgca ttccgtgcct atctgatgca aacattatgg cgaatccccg
      721 caattggtga acgcatgcgg gtttaccaac ttgcccgatt ctataaaaca ctcggtatgc
      781 tattacgcgg gggggtgccg attacgaaag cactggaaat ggtaagcgat ttattgcagg
      841 cgcacttcag gcctcgtgtt cgtgcagcag caagttttat tcgagaagga aaaaccatat
      901 caagtgcaat gcaaacggct gaactaacca ctgcggtagg aaatcgaatg ctccgcgttg
      961 gcgagcgcac aggtctcatg ggcgagatga tggagcggat tggaaatttt cacgatgaag
     1021 aaatttcacg ctgggtcgaa tgggcaacca aactcattga accaatatta atggcggtta
     1081 tcggaacggt gatcgggggc atcatcatct tgatgtatat gcctattttt gaattggcgg
     1141 gcagcatcaa ttgattgaat ccgccatgca ttcagaagca gtaacaaaac cattgcttga
     1201 tttagcacaa ttaacgcaag cccgccaaca agcaatggct aggaaaatta ccgttgtgaa
     1261 ggtactggaa gatgaaggtg gattttcacc ctacgacttg atgcaggagc ttgggcggtt
     1321 gcttcacatg ccggttctga aaatggaaga attacataca ctgcaaccgg tgtatgagga
     1381 attaactttt gctgaagcca tcacacagca atgtgtctta ttccagcgcg atgggaaata
     1441 tctactggcc gttggtgatc ccttcttacc caatttaaga tcttgggtag aagagtatat
     1501 ttctgttatc gccgaatggt acttagtgca ccccgctgat ctggctgcgt tttttgcgca
     1561 acaagaaaaa agtatgcgcg ccatggacaa tgtcttgcca gcagaacaac aaagctttgt
     1621 ccaggatggt atcgaagatt tatccttaaa aacgatacac gaaggaacca gtcatgtggt
     1681 cagactcgtt cattccactc tttatgacgc ccataaatct caggctagtg atattcacct
     1741 cgaaactgca cctggcgcct tggcaattaa gtatcgaatc gatggtgttc tgaccatgat
     1801 cggtgtaatg caaggaaccg acttagctga acaagtgatc tctcgcatca aagtcatgtc
     1861 ggatctcgat attgcagaac ggcgcgttcc acaagatggg cgctttaaag tttccattca
     1921 aggacgtgaa attgattttc gtgtttcgat catgcccagt atttttggtg aagatgccgt
     1981 actaaggatt ttagaccgac aagccctcgc agatcatgtc gaaggcttaa cgttgaatca
     2041 actggggttt gatcaatctg ccatcgctac gataaggcgt ttaagtgctg aaccctacgg
     2101 tatgctgtta gttaccggac caacgggaag cggaaaaaca acctcacttt atgccgcaat
     2161 ctcagaagtc aatcaagggg atgataaaat cattaccatt gaagacccca tcgaatatca
     2221 gctacccggt gtattgcaaa ttcccgtcaa tgaaaaaaaa ggactaacgt ttgcaagggg
     2281 cttgcgttct atactcaggc atgatcccga caagatcatg gtcggtgaaa ttcgagatgc
     2341 tgaaacagct caaattgcaa tacaagctgc gctgacaggg catctcgttt ttaccacagt
     2401 gcatgctaat aatgtgttcg atgtgattgg tcgattttca cacatgggcg ttgatcctta
     2461 tagctttgtc tcggcgttaa atggcattgt agcgcaacga ctagtgcgcc agctttgcat
     2521 gcattgtgtg gttgatgaac gaccgagtaa cgagatgatt cttgaatcgg gtatctcgct
     2581 ggaacaagct gagcaatatc atttcagggt cggcagaggt tgtgggcaat gtcgtggcag
     2641 tggctataaa ggtagaaatg cgattgctga aatattgtta ttgaatgatg aaatccgtga
     2701 gctgattatt tcgcaggcta ccattcgccg aattaaagaa gctgcaagac aaaatggaac
     2761 ccgctttcta agagaagccg ccttggatcg agtcaaagca ggacaaacga gcttacagga
     2821 ggctaatcgt gtcaccattg tggcgtgatc gaattcacat tttctttgcg ccaggcagga
     2881 ttgatttagt cagaattccc aagggactca aagctttacc gcagc
//
LOCUS       DAOQKQ010000229         2917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_23199_length_2917_cov_5.867925, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000229 DAOQKQ010000000
VERSION     DAOQKQ010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23199_length_2917_cov_5.867925"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PKZ49_11725"
     CDS             complement(<1..235)
                     /locus_tag="PKZ49_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HND37275.1"
                     /translation="MQKYFGYLVAVLSLVLTGCGYNTLQSTDEQIKASWSEVVNQYQR
                     RADLIPNLVNTVKGFAEQEKDVLLGVTNARSKVT"
     gene            391..1677
                     /locus_tag="PKZ49_11730"
     CDS             391..1677
                     /locus_tag="PKZ49_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HND37276.1"
                     /translation="MKIAIIGAGCSGLSAIKQLAAAGLTDITCFEKTDRLGGNWAYTE
                     KPGYSSLYQATRTISSKAMSQFSDFPMSADYPDYPSHEQILAYFQAYARYFGLEKFIR
                     FNLGVTQADKTLDDRWKLTLSDSTEQIYDYLVVANGHLTVPRHPDWKDQFLGTYLHVR
                     DYKTNRGFENKHVLVVGAGNSGCDCAVDIGRVAAQVDLSLRSPQYIIPKYIMGNPTDT
                     FASGFDWLPQWLQDGIQKMILRSQIGRYQDYQLPEPTFPPRQAHPTLNSELLPCMREG
                     KIKPRKAIDRIVDQTVYFSDGSFAQYDIILAATGYQIAFPFFPKGLMDWETSLSIPLY
                     LRMFDPQHANLAFIGLLQPQGCLWKLTEVQAKLLSLLLTQQIQLPENWRSLALTEGSD
                     WSKQFLTRPRHSLEVHYPAYLSQLQRIITGKILHDQ"
     gene            complement(1702..2703)
                     /locus_tag="PKZ49_11735"
     CDS             complement(1702..2703)
                     /locus_tag="PKZ49_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HND37277.1"
                     /translation="MKNDCSFSQQEVLTTIREFEKVLILYNPISSAGNTAELASDFED
                     FLIKQRFSVSVYETAKKMKSYQRIVADIAASNLIVVIGGDGTVRKLLPLLSQTHTPAY
                     VVPGGNESLIARSYGMTTQCTDLLQAMTTGKYLEQYYGLISGEGIQGKKPFFIMASMG
                     FDSLTIKRIGKRKGPINDAAYVWCGLNALVSLHHPVISVIVDGNTVIDQQSGYLIVAN
                     SAAYARNLQLVPEANPSNQELVLGFLPKAKHQHEWIKLTRMLQHKPAGLPLQYFSGKQ
                     ITLTLHHPSYPLQVDGDYFRNRDIEEGATIEFSIHPEPIRVLVPPDYCIQSTAIKSA"
BASE COUNT          824 a          672 c          586 g          835 t
ORIGIN      
        1 ccgtcacctt tgatcgggca ttggtcacac cgagcaagac gtctttttcc tgctcagcaa
       61 agcctttcac ggtattgacg agattgggta tcaaatccgc cctgcgttga tactgattca
      121 ccacttctga ccagcttgcc ttgatttgct cgtcggtcga ctgcaaggtg ttatagccac
      181 accctgtcag aacaagcgat aaaaccgcta cgagataacc gaaatatttt tgcatgccag
      241 gactcctctc cgattcgatt gatgctctta aaactaaagg aagcgttgct taaatgcaat
      301 cgttttattt aatcatctac agtttggttg ccgtcgtgca ttatactgac tggtacgcaa
      361 ccctttgtat ttcagctaaa tcacaaaccc atgaaaatcg ctatcattgg cgccggttgt
      421 tcgggtctta gcgccattaa acaactggct gctgccggct taaccgatat tacctgcttc
      481 gaaaaaactg atcggttggg tggaaattgg gcctacaccg aaaaacctgg ctatagcagc
      541 ctttatcaag ctactcgaac catttcgagc aaagcaatgt cgcagttcag tgattttccc
      601 atgtcggctg attacccaga ttaccccagt catgaacaaa tcctagccta ttttcaagca
      661 tatgcccggt attttgggtt agaaaaattt attcgattca atcttggcgt aacacaggca
      721 gacaaaacac tcgacgatcg ttggaaactc acattgagtg atagcactga gcagatctac
      781 gattatctgg tcgttgccaa tggtcacttg accgttccgc ggcaccccga ctggaaagat
      841 cagttccttg gtacctatct ccatgtacgc gattataaga ccaatcgtgg atttgaaaac
      901 aaacacgtac tggtagtcgg tgcaggcaac tcaggttgcg attgcgccgt ggatatcggc
      961 cgcgtagccg ctcaagttga tcttagtctg cgctctccgc aatatatcat ccccaaatat
     1021 attatgggaa atcccactga tacctttgct tcaggttttg actggttacc ccaatggctc
     1081 caggatggaa ttcaaaaaat gattctgcgc tcacaaattg ggcgttatca agattatcaa
     1141 ttaccggagc cgacttttcc gccgaggcaa gctcacccta ccctaaattc tgaattgtta
     1201 ccgtgcatgc gcgagggaaa aattaaaccc cgcaaagcaa tagaccggat tgtcgatcaa
     1261 accgtttatt tcagcgatgg atcctttgcg caatacgata tcatcctcgc agccactgga
     1321 tatcaaattg catttccttt ttttccaaag ggtttaatgg attgggaaac gtcgctctct
     1381 attccccttt acttaagaat gtttgatcct cagcatgcca atttagcctt tattggtttg
     1441 ctgcaaccgc aaggctgtct ttggaaacta accgaagtgc aagcgaaatt actcagccta
     1501 ttattaacac aacaaatcca acttcccgaa aattggcgct cgctcgcttt aactgaaggt
     1561 tctgattggt caaagcaatt tttaacccga ccaaggcatt cactggaagt acattacccc
     1621 gcttatctta gtcaattgca gcgcatcatt actggaaaaa tccttcatga tcaatagtga
     1681 taaggaatca accctttgcg actaggcgct cttgatagcc gttgattgga tgcaataatc
     1741 cgggggcacg agaacccgaa ttggttctgg gtgaatactg aattcaatgg tagcaccttc
     1801 ctcaatatca cgattgcgga aataatcacc atcaacctgt aagggataag aaggatggtg
     1861 aagtgttaag gttatctgtt tgcccgaaaa atattgcagg ggtaatccag ctggtttatg
     1921 ttgcaacata cgagtcagct taatccattc atgctgatgt ttggctttcg gtagaaagcc
     1981 cagaacaagc tcctgattgg atggatttgc ttcgggcaca agctgtaaat ttctcgcata
     2041 ggcagcgctg tttgcaacaa ttaaatatcc tgactgctga tcaatcacgg tatttccatc
     2101 gacgataacc gaaatcactg ggtgatgcag tgatactagt gcatttaaac cgcaccaaac
     2161 ataggccgca tcattgattg gccctttacg tttaccaatg cgcttgattg taagcgaatc
     2221 aaaccccatc gatgccatga taaaaaaagg tttttttcct tgaatacctt cacctgaaat
     2281 caatccatag tactgctcca gatatttccc tgtcgtcatg gcttgtaata aatcagtaca
     2341 ttgtgtagtc attccataag atcgtgcaat cagtgattca tttccgcctg gcaccacata
     2401 agctggcgta tgggtttgac tcaataaagg tagtagcttc ctgaccgttc catcaccgcc
     2461 aatcacgaca attaaattac tagctgcaat atctgccaca atgcgctgat acgatttcat
     2521 tttcttggcc gtttcataga ccgatacgga aaagcgctgt ttgatgagaa aatcttcgaa
     2581 atcgcttgct aattcagcag tgttgcccgc actggaaata ggattgtaaa gaatcaatac
     2641 tttttcaaat tcacggatag tagtcaaaac ttcctgctgc gaaaatgagc aatcgttttt
     2701 caatgaaatt gcctttaaat gtaaaccgag tagtggaatg aattctggac aaatacgagt
     2761 ttaagtaagc gtatcctaga ttttttacaa aatcatgtca caaccctatt gattcacttc
     2821 aagctaaaaa ctctgatcaa tcagaaaaag tgatagcaga atttttgtaa tgcttattca
     2881 ttattatgaa tttataaatg taaatgctta cgctaat
//
LOCUS       DAOQKQ010000230         2886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_23645_length_2886_cov_4.296715, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000230 DAOQKQ010000000
VERSION     DAOQKQ010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23645_length_2886_cov_4.296715"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /gene="metG"
                     /locus_tag="PKZ49_11740"
     CDS             <1..576
                     /gene="metG"
                     /locus_tag="PKZ49_11740"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HND37278.1"
                     /translation="VEKIEQFLNCDSLKWPTQAVGQPLSSLLLPAGHLINPYQHLMTR
                     IEPKQIEAMIAANKTPADTQASDTQSKVQPSDIAPIAETISIEDFSKIDLRIARIVDA
                     QHVEGAEKLLKLTLDIGIEQRTVFAGIKSAYDPEKLKGRLTVMVANLAPRKMKFGLSE
                     GMVLAASDPNVTGGLFILSPDEGAKPGMRVK"
     gene            complement(604..1155)
                     /locus_tag="PKZ49_11745"
     CDS             complement(604..1155)
                     /locus_tag="PKZ49_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HND37279.1"
                     /translation="MISHPRHQQIEFKLIGPVYRQFPTLKPITASVMLLTLIVSLMSG
                     CSTTTKTPTPSVSKSQSGAPYNRPYKVRGKTYYPMASAAGYRAEGIASWYGSESGNKT
                     AMGTRFNPQGLTAAHKTLPLPTKVKVTNLRNGRSVTVIVNDRGPFKKNRLIDLSHGAA
                     KQIGLQGLTEVRIESINTVASNP"
     gene            complement(1246..2631)
                     /locus_tag="PKZ49_11750"
     CDS             complement(1246..2631)
                     /locus_tag="PKZ49_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HND37280.1"
                     /translation="MNIQKIIQERYKENFSLHDRYLNSQMVKVLKTIGFDRHYVKAEG
                     AYLYDEKGNRYLDLLSGFGVFALGRNHPVIVQALQDVLSIDLPNLVQLDVSILSGLLA
                     EKLVSLSPKGLDRVFFANSGTETIEAAIKFSRYATGKPKIIYCRGGYHGLSLGSLSAT
                     GDTHYKEGFGPLVPDFIEIPFGDLSALEEALSQRDVAAFITEPIQGHGVWIPADDYLP
                     KAAELVRHYGALFIADEVQTGLGRTGKWWAIDHWSITPDIICMAKALSGGFVPIGALV
                     CRNWIFDRVFNRMDRAVIHGSTFGKNNLAMAAGLATLDVLESENLIDKSAQSGKILLD
                     ALQPLAQQYECVKEIRGKGMMIALEFKEPQSLSLRMSWKILEAANKGLFSQLITVPLF
                     ARHRILSQVAGNGMNIVKFIPPFILNQSDLNWIINAVQDVVIAAHRGPKAVWDLGKVF
                     ATKALRMKAGS"
BASE COUNT          815 a          604 c          657 g          810 t
ORIGIN      
        1 gtagaaaaaa ttgaacaatt cctgaattgt gattccttaa aatggccaac tcaggcggta
       61 ggacaacctt tgtccagttt attactacct gcaggccact taatcaatcc ttaccaacat
      121 ctcatgacac gaatcgaacc gaaacaaatc gaagccatga ttgcagcgaa taaaaccccc
      181 gctgatactc aggcctctga tacgcaatca aaagttcaac catcggatat cgcaccaatc
      241 gcagaaacta tctcgattga ggacttcagt aagatcgatt tacgtattgc ccgaatcgtt
      301 gatgcgcagc atgtggaagg ggcagagaaa ttactgaaac ttacgctgga tatcggtatt
      361 gagcagcgta ccgtatttgc tggtattaaa tcagcctacg atcctgaaaa attaaagggg
      421 cggctaaccg tcatggtggc taatttagca ccccgcaaaa tgaaattcgg tttgtccgaa
      481 ggaatggtac tcgcagcgag cgatccaaat gttacgggcg ggttgtttat tctctcaccc
      541 gatgaaggtg ctaaacctgg gatgcgagtc aagtaatcct tgatgataca accggtggct
      601 gtttcaagga ttgctagcaa cggtattgat cgattcaatt ctaacttccg ttaatccctg
      661 caatccaatt tgtttggcgg caccatgcga tagatcgatt aatcgatttt tcttaaatgg
      721 acctcggtca ttgacaatga cagtgactga gcgaccatta cgcagattcg tcacttttac
      781 cttggtgggg agtggtaggg ttttgtgggc tgccgtcagg ccttgtggat tgaaccgtgt
      841 acccatcgcg gttttattgc ccgactctga accataccaa gaagcaatgc cttcggcacg
      901 atagccagca gccgatgcca tcggataata agtttttcct cttactttat aaggtcggtt
      961 gtagggcgca cccgattgtg atttcgatac agatggcgta ggtgttttag ttgtcgttga
     1021 acacccactc attaagctga caatcaatgt gagcaacatc accgaagcag tgataggttt
     1081 taatgttggg aattgacggt agacggggcc tattaatttg aactcaattt gttggtgtct
     1141 tgggtgacta atcatgtgtt tatttttatt actgtcatca atgctgaaat taaatcatag
     1201 aaatagggtt ttaaaaacaa ggaaatttaa gtcataactg caatatcaag agcccgcttt
     1261 cattcttaat gcctttgttg caaaaacttt accgagatcc caaactgctt tgggtccacg
     1321 gtgtgccgcg atgaccacat cttgaacggc gttaatgatc caattgagat ccgattgatt
     1381 gagaatgaac ggcgggatga atttgacgat attcatgcca ttgcctgcta cttgagacag
     1441 aatgcgatgc cgtgcaaata gaggtaccgt aatgagttga gaaaataatc ctttattggc
     1501 ggcttctaaa attttccagg acattctgag cgataagctt tggggttctt taaattcaag
     1561 cgcgatcatc atgcccttac ctcgaatttc tttgacgcat tcgtattgct gagcaagggg
     1621 ttgcagcgca tcgagtagga tttttcccga ttgtgcggat ttatcgatta ggttttccga
     1681 ttctaaaaca tctaaggtag ctaaccccgc tgccatcgcg aggttattct ttccaaacgt
     1741 actaccatgt atcacggcgc gatccatacg attgaatacg cgatcaaaga tccaatttct
     1801 gcaaacgagt gcgccaatgg ggacaaaacc acccgaaagg gcttttgcca tacaaatgat
     1861 atcgggggtg atagaccaat ggtcgatcgc ccaccatttt ccagttctgc ctaatccggt
     1921 ttgaacttca tcggcaatga ataatgcgcc atagtgacga acgagttctg cagctttggg
     1981 tagataatca tcggcgggga tccatacacc gtgtccttgg atcggttctg taatgaaagc
     2041 agcgacatct ctttgggaaa gcgcttcttc gagtgcggat aaatcgccga aaggtatctc
     2101 aatgaaatca ggaactaagg gaccgaatcc ctctttgtaa tgggtatcac ccgttgctga
     2161 gagtgaacca agacttagtc catgataccc gccgcgacag tagatgatct tcggttttcc
     2221 agtcgcgtaa cggctaaatt taattgcagc ctcaatggtt tcagtgcctg agttagcaaa
     2281 gaaaacacga tctagccctt tcggactcag tgacaccaat ttttcagcca gcaatcctga
     2341 taggatagag acatcgagtt gcacgagatt gggtaaatca atacttaata catcttgtaa
     2401 cgcttgtaca ataaccgggt gattacgacc taacgcaaaa acaccgaaac cactgagcaa
     2461 atcgaggtag cgattgcctt tttcatcata taaataggca ccttcggctt taacataatg
     2521 tcgatcaaac ccgattgttt tgagcacttt caccatttgg ctattgagat agcggtcgtg
     2581 gagtgaaaaa ttctctttgt aacgttcttg gatgatcttc tggatgttca tagactaaat
     2641 ggccaaatat gtggtttcag gttataagtg ctgatgattg tgagcgattg agcaacggtg
     2701 aaataggaat ttgtaattaa acttggtaca catcagctaa tagggtattt aaagcaaagc
     2761 gatagggtaa cagcaattgg gagatataac ctagattagt ctatgcagat attgattaca
     2821 aagtgtcaca attgttgtgg aaattttcaa agcattatta tggtgtctcc ctaagactgt
     2881 caatag
//
LOCUS       DAOQKQ010000231         2883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_23687_length_2883_cov_5.554455, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000231 DAOQKQ010000000
VERSION     DAOQKQ010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23687_length_2883_cov_5.554455"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            534..1352
                     /gene="coxB"
                     /locus_tag="PKZ49_11755"
     CDS             534..1352
                     /gene="coxB"
                     /locus_tag="PKZ49_11755"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HND37281.1"
                     /translation="MSSKSVATRVGAALLTFYSSLAMSSKYNFPEPQSAIAQDVYDQH
                     IFAMWICLAIFIAVFGVMFYSILKHRKSLGYKAANFHHSTLVEIIWTAIPCAILVVMA
                     LPATKTVLAMKDTSEPDITIKVTGYQWMWGYDYLQGEGEGISFYSKLSTPRAQIENKE
                     PKGENYLLEVDNNLVVPVGKKVRVIVTANDVIHAWWVPAFSVKQDAIPGFVRDAWFKA
                     DRTGIFRGQCTELCGKDHGFMPIVVEVVEPEKYAQWVADKQKASASAMTTAALN"
     gene            1405..>2883
                     /gene="ctaD"
                     /locus_tag="PKZ49_11760"
     CDS             1405..>2883
                     /gene="ctaD"
                     /locus_tag="PKZ49_11760"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HND37282.1"
                     /translation="MAVVQDVHGHGHDDHHPTGIMRWVCTTNHKDIGTMYLWFSFAMF
                     LVGGFLAMGIRAELFMPGIQILEPELFNSFTTLHGLIMVFGAIMPAFVGLANWQVPMM
                     IGAPDMAFARMNNWSFWILPVAATLLVSSFFVPGGAAAGGWTFYPPLSTQGGMGVDMM
                     IFAVHLLGASSIMGAINIITTILNMRAPGMTLMKMPLFVWTWLITAYLLVLVMPVLAG
                     TVTMLLTDRNFGTSFFNAAGGGDPVLFQHIFWFFGHPEVYIMILPAFGVVSEIIPTFS
                     RKPLFGYSSMVYATASIAILSCVVWAHHMFTVGMPSVGQLFFMYATMLIAVPTGVKVF
                     NWTATMWRGSMTFEVPMLFSIGFIFLFVIGGFSGVICAIVPIDIQVQETYYVIAHFHY
                     VLVSGALFAIFGAVYYWLPKWCGRMYDEKLGKWHFWLSMFFFNLTFFVQHFLGLAGMP
                     RRVPDYALQFADFNMVSSIGGFGFGLTQLFFLYIVIKCIRSGE"
BASE COUNT          757 a          461 c          666 g          999 t
ORIGIN      
        1 tatctttaca attaccacaa ttcgtatgag gttatttggg tgagggcgat ttgagaaaat
       61 gcgaaaatta gctcagaaat ctgacgataa acagtcatct attgaaaatt tctaattatt
      121 cttatatgag aataacttat ttatttttat ctaaataagt catcaatctc ttctgcaaaa
      181 taagtgcttg gtatttattt tgaccagatt cgcattttgt ttttttctca ttatctaatt
      241 ttagtgtgct gctaatttgg aaattaccct aattttgata gggtaatttc cctataaaaa
      301 tcatgatttt cccttgtttg agcatactca gcatgataga atcttaacct ggttcagaat
      361 ggtagtctgg taaaaaaact atctagatat cttgatagat aactttgttt tagttttata
      421 gctaaattta ctttgagtta aggtaattaa aagaattagc tattaagagt tttaaagttt
      481 ttggtttgtc aaaatagaca agtaagtttg tattaatatg tgggggggaa tatatgagta
      541 gtaaatcagt agcaactcga gtgggggcgg cgcttcttac tttttactcg agcttagcga
      601 tgtccagtaa atataatttt ccagagccgc aatcggcaat tgctcaagat gtctatgatc
      661 agcatatatt tgcgatgtgg atttgcttgg cgatttttat tgcggttttc ggcgtaatgt
      721 tttattcaat tctgaagcac cgtaaatctt tagggtataa agcggccaat tttcatcaca
      781 gcactttagt tgagattatc tggacagcaa ttccttgtgc aattttggtt gtaatggctt
      841 tgccagcaac aaaaacagta cttgcgatga aagacacttc agagccagac ataactatca
      901 aagttactgg ctatcaatgg atgtggggct atgattatct ccaaggtgaa ggtgaaggaa
      961 taagctttta cagtaaatta tctacaccaa gagcgcaaat tgagaataaa gaaccgaaag
     1021 gtgaaaatta tttgttagaa gtggataata atctcgttgt gccggttggg aagaaagtaa
     1081 gggtgattgt aacggctaat gatgtaattc atgcttggtg ggttcccgcg tttagtgtta
     1141 agcaagatgc aattccagga tttgtgcgtg atgcctggtt taaggctgat cggacaggga
     1201 tattccgtgg gcagtgtact gaattgtgtg gaaaagatca tggtttcatg ccgattgttg
     1261 ttgaggtggt tgagccagaa aaatatgctc agtgggtagc tgataaacaa aaagcttcag
     1321 catcggcgat gacaacagcg gctttaaatt aattatttag tttttttgta atataaatag
     1381 tagaaacact aaggagagta aatcatggca gtagttcaag acgttcatgg tcatggtcat
     1441 gatgaccatc acccaacggg gataatgcgt tgggtttgta caacaaacca taaagacatc
     1501 ggaaccatgt atctatggtt cagttttgca atgtttttgg taggtggatt cttggcaatg
     1561 gggataagag ccgagttatt tatgccgggt attcaaattc tagagcctga gctgtttaac
     1621 tcttttacca cattgcatgg gttaattatg gtattcggtg ccatcatgcc tgcgtttgta
     1681 gggttggcaa actggcaagt accaatgatg attggtgcgc ctgatatggc atttgctcgt
     1741 atgaacaact ggagtttctg gattctgcct gtggcggcta ctttgttagt aagctccttc
     1801 tttgttcctg gcggtgctgc tgctggaggt tggacattct atccgccact gtcgactcaa
     1861 ggtggtatgg gcgttgatat gatgattttt gcggttcact tgttgggcgc atcatcaatc
     1921 atgggcgcta ttaatatcat cacaacgata ttgaatatgc gcgcgccagg gatgaccttg
     1981 atgaaaatgc cattatttgt ctggacttgg ttaattacgg catatttgct ggtgttggtg
     2041 atgcctgtgt tagctggaac tgtcaccatg ctgttaacag accgtaactt tggtacaagt
     2101 ttcttcaatg cggcaggtgg tggtgatcct gtattattcc agcatatttt ctggttcttt
     2161 ggtcatccag aagtgtatat catgattctg ccagcatttg gggttgtatc tgaaatcatt
     2221 ccaacattct ctcgtaaacc attgtttgga tattcttcaa tggtttatgc aacggcttcg
     2281 attgcaattt tatcttgtgt cgtttgggcg catcacatgt ttacagtggg catgccatca
     2341 gtcggtcagc tattcttcat gtatgcaact atgttgattg ctgtgccaac tggtgtgaaa
     2401 gtatttaact ggacggcaac aatgtggcgt ggttcaatga cttttgaagt gccaatgctg
     2461 ttttcgattg gatttatctt cttatttgta attggtggtt ttagcggtgt gatttgtgca
     2521 atcgtgccaa tcgatattca agttcaagaa acctactatg taattgcaca tttccactat
     2581 gtgcttgtat caggtgcgct atttgcaatc tttggtgctg tttattattg gttacctaaa
     2641 tggtgtggtc gtatgtatga tgaaaaattg ggtaaatggc atttctggtt atccatgttc
     2701 ttcttcaact tgactttctt tgtgcaacat ttcttaggat tagcaggcat gccacgcaga
     2761 gttcctgact acgctttgca gtttgctgat tttaatatgg tatcaagcat tggtggtttt
     2821 ggatttggct taactcagct attttttctg tatatcgtaa tcaagtgtat tcggagtgga
     2881 gag
//
LOCUS       DAOQKQ010000232         2865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_23941_length_2865_cov_7.181139, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000232 DAOQKQ010000000
VERSION     DAOQKQ010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23941_length_2865_cov_7.181139"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            298..1182
                     /locus_tag="PKZ49_11765"
     CDS             298..1182
                     /locus_tag="PKZ49_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37283.1"
                     /translation="MNPFSVDHISCSNKQDHFNRYPMAKSTVTAYACLHRRIALLFLL
                     GLIYLSGCETVPLQDRNYADRGGFWEQGTSTQARFHHQAFDKDDEIGVMYASGRNTIL
                     NGSAINLSSDLDNHSVISNGPQSSARLEFKGDDEDDGDTCVINIDRFNRGDAYADTYD
                     CWYHIKTPHALLKAKNAILHIDVTQNETEVVVISGTIKVFTHGDPIQSIDVNSDQEVI
                     ISHQMLHRPRSISPDEIWNKISWRAQFQLYKTVIDWDQVVRGAIIVGIVAAIVAIGVA
                     TKGVGLSGLHRSRGFSRH"
     gene            complement(1179..2621)
                     /locus_tag="PKZ49_11770"
     CDS             complement(1179..2621)
                     /locus_tag="PKZ49_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37284.1"
                     /translation="MEPLVHFDKIIVEKPKVSKDCFYTNYILVRKGKSITCELRHKYE
                     ESIEGDNAQRYANLTSGIPAINHGLFAKEICFEFDLGPKEYNFFDEMLKKMSIEIFVS
                     RFYHDPLFIKPAFIAKQADVKLSNAMCQAQFTAGIGHDIANYPADPDKIVVLSSGGKE
                     SLLCYGLLNKAGMDVYPIYINESGGHWRTALTAYRSFKQTDTKTKRIWSNIDRLYAFM
                     VRNSPVVENNIRGIKSITDPLHFFTFAHYVFASLPLMEKSRIGHVCVGNEYVEPTKIL
                     TERHKINDVEFFPGIYDQSQAFDRYMTEWFESAGYGIKQWAPVRTITGYQVQKTLALD
                     YPGLFKLQTSCHHAHFKNGKILPCGTCQKCMRILAFCIANQLDYTLIGYTADNVAWLQ
                     SNIHRLVLAGDQELEHTFYQLGQQGFTVKGQRHDHIEQLHFDTHSSDVSNVPLQWRNR
                     IYPLLLGKATGMVQLTEDHQWQSIDIHEIS"
BASE COUNT          857 a          581 c          585 g          842 t
ORIGIN      
        1 tgctggctca agcgttacac catatttcag tttctgtgcc caaaaaatta agcggactaa
       61 caatggatac tgattaatgg cttttttagt gactcgcgac atgtaattag actcctcttc
      121 tgttcttgcg tgatctccat tcgatcactt aatctttagg gttttatcct agctttttcc
      181 aatctccctg aatatgtatt tttaacgcct tattcagtcg cgattcagat agatgagcgt
      241 agagtaatct ccatcaatca ttcatctaaa ccgaatcaga ctgagaggag cagcaaaatg
      301 aatccgttca gcgtagatca tatctcttgc agtaacaagc aagatcattt taaccgatat
      361 ccaatggcta aaagcacagt aacagcatat gcatgcttac atcgccgcat tgcactacta
      421 tttttattgg ggttgatata tctaagcggt tgcgaaacag tcccattaca agatcgcaat
      481 tatgccgata gaggggggtt ctgggagcaa ggcacttcta cccaagcacg ctttcatcat
      541 caggcttttg ataaagatga tgagattgga gtaatgtacg catccggccg caataccata
      601 ttaaatggat cggctatcaa cttatcttca gatcttgata atcactcagt catcagtaac
      661 ggaccgcaaa gttcagcccg actcgaattt aagggtgacg atgaagatga tggggacacg
      721 tgtgtaatta atatcgaccg atttaatagg ggagatgcct acgccgacac ctacgattgc
      781 tggtatcaca tcaaaacacc gcacgcacta ttaaaagcta aaaatgcaat actacatatt
      841 gacgtgactc aaaatgaaac ggaagtagtc gttattagcg gcaccattaa agtgtttact
      901 catggagacc ccatacaatc tatcgatgta aacagtgatc aagaagtcat catttcgcat
      961 caaatgctcc atcgcccacg ttccatttca cccgatgaaa tttggaataa aatcagttgg
     1021 cgtgcgcaat ttcaattgta taaaaccgtc atcgattggg atcaagtagt gcgtggcgct
     1081 attatcgttg gaattgttgc agccattgtc gcaattggcg tggcaaccaa aggtgtggga
     1141 ttgagtggac ttcatcgatc tcggggattt agcagacatt aacttatttc gtggatatct
     1201 atcgattgcc attgatgatc ttcagtaagt tgtaccatgc cggtagcttt tccgagtaat
     1261 agtgggtaaa tcctatttcg ccactgaagc ggtacattgc taacatccga tgaatgtgta
     1321 tcgaagtgaa gttgctcaat atgatcgtgc cgttggcctt taacggtaaa gccttgttgg
     1381 cctaattgat agaaggtatg ctccaactct tgatcacccg ctaataccaa tcgatggata
     1441 ttggattgca accaagctac gttgtctgca gtatatccaa tcagggtgta atccagttga
     1501 ttggcgatac aaaatgctaa aattcgcata cacttctgac acgtgccgca tgggagtatt
     1561 ttgccatttt tgaaatgggc atgatggcag gaagtctgta atttaaataa tccaggataa
     1621 tctagcgcta gcgttttttg cacttgatag ccagtgatag tgcgaactgg cgcccattgt
     1681 ttgataccgt accctgcgga ttcaaaccat tcggtcatat aacgatcaaa tgcctgactc
     1741 tgatcgtaga ttccgggaaa aaattcgaca tcattgatct tatggcgctc agtcaaaatt
     1801 ttagtaggct cgacatattc attaccaacg caaacatgac caattcttga tttttccatg
     1861 agtggcaacg aagcaaatac ataatgcgca aacgtaaaaa aatgcagcgg atcagtaatg
     1921 gatttgatac cacgtatatt attctcgacg accggtgaat ttctcaccat aaacgcatac
     1981 agccgatcaa tatttgacca aatacgttta gtcttagtat ccgtttgttt gaatgatcga
     2041 taagctgtaa gcgctgttcg ccaatgacca ccagattcat tgatataaat gggataaaca
     2101 tccatacctg ctttattgag taaaccatag caaagtaaac tctcttttcc accggaagaa
     2161 aggaccacga tcttatcggg atcagctgga taattagcaa tatcatggcc gatacccgct
     2221 gtgaattggg cttggcacat agcattggaa agttttacat cagcttgctt ggcgataaag
     2281 gcaggtttga taaaaagtgg atcgtgataa aagcggctta caaaaatctc gatactcatc
     2341 ttcttgagca tctcatcgaa gaaattgtat tctttgggcc ctagatcaaa ctcgaagcaa
     2401 atttctttag caaaaagccc atggttgata gctggaatac ccgatgtcaa attggcgtag
     2461 cgctgcgcgt tatctccctc gatactttct tcatatttgt gccgaagttc gcaagtaata
     2521 gatttacctt tgcgaacaag gatatagttg gtgtaaaaac aatccttcga aactttgggt
     2581 ttttcgacaa tgattttatc gaaatgaaca agcggttcca tactaggaat aaatacctaa
     2641 tcgtaagtag aaataaagat tctactgaat cacttgttaa gtcattgtgt agataacttc
     2701 gaaaattgaa tttagctttt ttagcaggtt aataaaggag tgtatttaga aatctattgt
     2761 cacgttgaac ctgactattt ggctacagaa aacctacgat gatcaattcg ttatatgcct
     2821 ctaaactatt ttcagtttaa tctgggtgtg actcaaacag gcatt
//
LOCUS       DAOQKQ010000233         2821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_24659_length_2821_cov_5.275850, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000233 DAOQKQ010000000
VERSION     DAOQKQ010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24659_length_2821_cov_5.275850"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /gene="fmt"
                     /locus_tag="PKZ49_11775"
     CDS             <1..774
                     /gene="fmt"
                     /locus_tag="PKZ49_11775"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HND37285.1"
                     /translation="ASEYSIPVLQPVTLKSEVTQEQLRSFAADVMIVAAYGLILPESI
                     LQLPLQGCLNIHASLLPRWRGAAPIQRAIQAGDQETGITIMQMDQGLDTGDILLQRTI
                     PIDPSDTAQSLHDNLAQLGAQCIIEALGLLQQGKLLAVEQNDEEVCYARKIEKDEAKI
                     DWSLDAAVIDRNIRAFNPFPVAFTSYQSLSLKIWQARIEQKLSGQPGEIVNIDNDGIT
                     VACGKHAIVLEVIQRAGGRKLPVKDFLKGNPMKRGEFMV"
     gene            859..2136
                     /gene="rsmB"
                     /locus_tag="PKZ49_11780"
     CDS             859..2136
                     /gene="rsmB"
                     /locus_tag="PKZ49_11780"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HND37286.1"
                     /translation="MMKIQQLAVDSIEIVLSGKNLTLVLQALWHHNPLLSLQQRGAIQ
                     DIVYGVLRYLGQLEGVLSQLVKRPLSDKRLRNLLLVSLYQLHYSKSPDYAIVDCAVAT
                     TRKLTKNKGSAGLVNAILRNFLRNDTALLDLANAHETGRFSHPQWWIDKLRYQYPQQY
                     EAILLANNHHPPMTLRVNRHKTSVIDYQALLNRCEIESCQVWDNALMLSKPVPTQKLP
                     MFEQGYVSIQDAGAQLAAPFLDIRDGMTVLDACAAPGGKSTHLMEVANIQLTVLDNDQ
                     QRLGRVSENFKRLGIEPFQQVCGDAALPSSWWDGKCFDRILADVPCSASGVVGRHPDI
                     KWLRRITDLATFANMQQNILDALWPLLKSEGKLLYVTCSVFFEENGQQVTNFLDKHPD
                     ARLLPIVHNEVNNGQLIPNSSHDGFYYASFYKI"
     gene            2114..2737
                     /locus_tag="PKZ49_11785"
     CDS             2114..2737
                     /locus_tag="PKZ49_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HND37287.1"
                     /translation="MPHFIKYNGMNWLERLVSMLILGGVLIGVTIQSAHSEGNIKIKS
                     FALEATDAGYQINVDAAIELNSTLEQALEKGIVLYFVIKFALIDTKWYWVDDEVARSK
                     LRIGLSYNALTRQYRLSHRSKSQGFDTLIDALQELGTQHYIPVEEKSELKPNMEYLAS
                     LQVWLDASRLSKPFQLESLSSKEWNLSSEKKEWRTKLPAAPSFSIQR"
BASE COUNT          818 a          528 c          670 g          805 t
ORIGIN      
        1 gcaagtgaat attcaattcc agttttacaa ccggttactt taaaatcaga ggtaacgcaa
       61 gagcagctaa ggtcttttgc ggcagatgtt atgattgttg ctgcctatgg acttatcttg
      121 ccagaatcaa ttttgcagtt accgttacag ggatgtttaa atatccacgc ctctcttctg
      181 ccgaggtgga ggggtgctgc cccgatccag cgcgctattc aagctggtga tcaagaaacg
      241 gggataacga tcatgcaaat ggatcagggt ttagatacgg gtgacatatt attgcagaga
      301 accattccaa tcgatccatc cgatacggcg caatcattgc acgacaatct agcgcaatta
      361 ggtgcgcaat gtattattga agcgcttggt ctattacaac aaggaaaatt gttggccgtt
      421 gagcagaatg atgaggaagt ttgttatgcg cgtaaaattg aaaaggatga agcaaaaatc
      481 gattggagtc ttgatgcagc ggtgatcgat cgcaatattc gcgcatttaa tccgtttccc
      541 gttgctttta ctagctatca atcgctatcg ttgaaaatat ggcaagcacg aattgaacag
      601 aagctttctg ggcagcccgg tgagattgtt aatatcgata atgacggtat taccgttgct
      661 tgtggtaagc acgcgatagt tctggaagtg attcaaagag ccggggggag aaaattacca
      721 gtaaaggatt ttcttaaagg gaatcccatg aaacggggtg agttcatggt ttagtcgtgc
      781 ttcggtcact gattaaccca ttctgtagtt taactagaat tagggcattt aggagggagt
      841 agcatagctg atattggtat gatgaaaatt cagcagcttg ctgtagattc aatcgaaata
      901 gttttgtctg ggaaaaattt aaccttggta ctacaagcgc tctggcatca taatccgtta
      961 ctatcattgc agcagcgcgg tgccattcaa gatatagttt atggtgtgct gcgatattta
     1021 ggccaactgg aaggggtgtt gagccaatta gtaaagcgac ccttgtcaga caaacggttg
     1081 cggaatctac tgctggtaag cttatatcag cttcattata gtaaatcacc tgactacgca
     1141 attgtagatt gtgctgtcgc aacgactcgg aaattaacta aaaacaaagg ttcagcaggt
     1201 ttggttaatg cgattctaag aaatttttta agaaatgata cggctttgtt ggatctagcc
     1261 aatgcacatg aaacaggaag attttcgcat ccacaatggt ggatagataa attaagatat
     1321 caatacccac agcagtatga agcgattttg ctggcgaata atcatcatcc tcccatgacg
     1381 ttgcgagtga atcgacacaa aaccagcgtc atcgattatc aagcgctact taatcggtgt
     1441 gagattgaaa gctgccaagt atgggataat gcacttatgt tatctaagcc agtacccaca
     1501 caaaaattac cgatgtttga gcaaggctat gtttcgatcc aggatgcggg ggctcaatta
     1561 gctgcgcctt ttctggatat tcgcgatgga atgactgtcc ttgatgcatg tgcagctccc
     1621 ggggggaaaa gcactcattt aatggaagtg gcaaatatcc aattgacggt tctcgataat
     1681 gaccaacagc gtttgggtcg cgtgtcagaa aattttaagc gattaggaat tgaacccttt
     1741 cagcaagtct gtggggatgc ggcattacca tcttcatggt gggacgggaa atgctttgat
     1801 cgaattctgg cagacgtccc ttgtagtgct tccggagtag ttggccgaca ccctgatata
     1861 aagtggctgc ggcgtatcac cgatcttgct acgtttgcga atatgcaaca aaatatttta
     1921 gatgcattgt ggccacttct taaaagtgaa gggaaattgc tctatgtgac ttgttctgtt
     1981 ttttttgaag agaacggtca gcaagtaacg aatttcttgg ataaacatcc tgatgctcgc
     2041 ttacttccaa tcgtacataa tgaagttaat aatgggcaat tgatacctaa ctcctctcat
     2101 gatggttttt attatgcctc attttataaa atataacggc atgaattggt tggaaagact
     2161 ggttagtatg ctgatattag ggggggtact cattggtgta accattcagt cggcccatag
     2221 tgaaggcaac ataaaaatta aatcatttgc attggaggca accgatgctg gttatcagat
     2281 taatgttgat gcggcaattg aattaaatag caccttagag caggccctcg aaaaggggat
     2341 tgttttatat tttgtgatta aatttgcatt gattgatacc aagtggtact gggtggatga
     2401 tgaggtagca agaagtaaat taagaatcgg cctaagttat aatgcactaa cccgccagta
     2461 tcggctgagc caccgatcca aatcgcaagg gttcgataca ttaatcgatg cacttcaaga
     2521 gctgggcact cagcattata ttccggttga agagaaatcg gagcttaagc ccaatatgga
     2581 atacttagct agcttacaag tatggcttga tgcttctcgc ttatctaagc ccttccaact
     2641 tgaatcattg agttccaaag aatggaactt gagttcagaa aaaaaagaat ggcgcactaa
     2701 actgcctgca gcgccatcat tttcaattca aagataatca gtgaaatatc ttatccttct
     2761 cgttacagga ttaagtgtcg tcatgctgtt tctcttggca acggcagggg cgaataccga
     2821 t
//
LOCUS       DAOQKQ010000234         2798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_25020_length_2798_cov_4.475392, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000234 DAOQKQ010000000
VERSION     DAOQKQ010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25020_length_2798_cov_4.475392"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /locus_tag="PKZ49_11790"
     CDS             complement(<1..1190)
                     /locus_tag="PKZ49_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HND37288.1"
                     /translation="MTTSQTLPQKITKSYVDKLSVPETGQAFIRDTELKGFAVRITSS
                     GAKSFILEKRINGKVKRLTIGRYPEITVEQARKEAHKLLGHIATGRNPIAEKKQEALQ
                     GTTLKQAFDDFVKTRKNLKERTLYDYRRVMAIVFDDWQDKALVNINKDMVSKRHNKIG
                     VERGEAYANLSMRFLRALFNYAIAQYEDGYGKSVLHDNPVTRLTQTRAWYRIERRQTV
                     IKPYELKPWFAAIMNLKNDPISQNRETIRDYLLLVLFTGLRREEAASMTWSNIDLQAR
                     TLKVTDTKNHSDHALPLSDFLYDLLRKRKEQAINEYVFHRANGIGYVTEQRKQIAKVI
                     KESGITFTIHDLRRTFLTIAESLDIPAYAVKRLANHKMSNDVTAGYIVTDVERLRQPM
                     QKIT"
     gene            1737..>2798
                     /locus_tag="PKZ49_11795"
     CDS             1737..>2798
                     /locus_tag="PKZ49_11795"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HND37289.1"
                     /translation="MTYLYNQPDMFVEEMIQGFAAAHRDLIAAVNGGVVRRYQAEPGQ
                     VVVIIGGGSGHYPAFGGLVGPGLVHGAAMGNVFASPSAQQIYDVALAAYAGGGIMLCY
                     ANYAGDVLNFNQAQARLQAQGIDVRAVVVTDDIASAPLAERQLRRGIAGALPVFKVAA
                     AAADSGESLDEVCRIANEANTRVRTLGVAFSGCTLPGAQQPLFHVEHGKLAVGMGIHG
                     EPGLYQQDGLPADDLAAMLVEKLLQDISPVGDLPKGTHISVLLSSLGSVAQEELFVVY
                     RKVDQLLHSRGLIIVEPVVDRVITSFDMAGISLTLFWLNDELETYWKAAADTPAFKRG
                     TISCFRPETDAALIPQVAVA"
BASE COUNT          721 a          529 c          669 g          879 t
ORIGIN      
        1 tcggtaattt tttgcatagg ttgtcgcaat cgttccacgt cagtaacaat gtatccggcg
       61 gtaacatcgt tactcatttt atgattggct aaccgtttca cagcataggc tggaatatca
      121 aggctctcag cgattgttaa gaaagtgcgt cgtaagtcat gaatagtgaa agtaatgcct
      181 gattctttga taactttggc tatttgtttg cgttgctcgg taacatatcc gattccattg
      241 gctcgatgga atacatattc attaattgct tgttcctttc tttttctcag taaatcgtaa
      301 agaaaatcgg ataacggtaa ggcgtggtct gaatgattct tagtgtcagt tactttgagt
      361 gttctggctt gtagatcgat attgctccag gtcatgctgg ccgcttcttc tcgtcttaat
      421 ccagtaaata gtacgagtag caaatagtct cggatggttt ctcgattctg actgatagga
      481 tcattcttta gattcataat cgctgcgaac cagggcttaa gctcgtaagg tttaataaca
      541 gtctgacggc gttctattcg ataccaggca cgagtctggg taagacgtgt tactgggttg
      601 tcatgtagaa cagattttcc ataaccatct tcatattgag cgatggcgta attgaacaaa
      661 gcgcgtaaaa aacgcatgga taaattagca taagcttcgc ctctttctac tccaatcttg
      721 ttatggcgtt tgctgaccat atccttgtta atattaacca gtgctttatc ctgccagtca
      781 tcgaatacga tagccatcac acggcgatag tcatagagcg tgcgttcttt gaggtttttt
      841 cgcgtcttaa caaaatcatc gaaagcctgt tttagcgtag tgccttgtaa ggcttcctgt
      901 tttttctcag cgatgggatt acgacccgtg gcaatatgac ccagcaattt gtgagcttct
      961 tttctggctt gttctacagt tatctcagga tagcggccta ttgttaatcg ctttactttg
     1021 ccgttgattc ttttttctag aatgaaagat tttgcaccgg atgaggtgat tcgcacagca
     1081 aaacctttta gttcagtatc gcggataaaa gcttggcctg tttctggaac agataatttg
     1141 tctacataag attttgtgat tttttggggg agagtttggg aagtagtcat tgttttaatt
     1201 tcattccatg tagacactat gtagacagaa aatgtaaaac tatgggttag agtttaaaac
     1261 agtatgatag gaaatatcaa tacaaaacaa tgacattaat acattctaaa aaatattaaa
     1321 actacgtaaa acagtatatt ttagaattgg taatcagtag gtcggaggtt cggctcctct
     1381 caacagcacc aaaaatcaat aagttacagt tagtttatag attgataatt ttatctatgt
     1441 aactaaaacg taactaacac aaaaaggtac ctggtcttgg tcttgcaata caacgtatct
     1501 ctaagaagtc atgaaacctg ttgtaatcgt accaaagcat aataaatgtg aacaagtagc
     1561 tttccacagc aatgtcatgc actttttttc tgacgaatca gtaataattg aagatgattt
     1621 gtagctcggc tatttgagaa tagagttagt atttgtggcg cagggtaatc aatcgatatt
     1681 ttttctgatt taatatgtga tacatataac gttccttcta ataattttga ttgattatga
     1741 cttatcttta taaccagcct gatatgtttg tagaggagat gattcaaggg tttgcagcag
     1801 cccaccgtga tcttatcgcc gcagtcaacg gtggtgtggt tcgtcgttac caagcagaac
     1861 ccgggcaggt agttgtcatc atcggagggg gttctgggca ttatccggcg tttgggggat
     1921 tggtaggacc tgggttggta catggtgctg caatgggtaa tgtatttgcg tcaccttctg
     1981 cgcagcaaat ctatgatgtt gctctggcag cctatgcagg gggtggtatc atgctttgct
     2041 atgctaatta tgcgggtgat gtgctcaatt ttaatcaagc gcaagcgcga ttgcaggcac
     2101 agggaattga tgtgcgtgca gtggtagtga ccgatgatat tgcgtcagcg ccactagcgg
     2161 agcgccagct acgccgtggt atcgctggag cattgccggt attcaaggtg gcggctgcag
     2221 ccgctgatag tggagaatcg ttggatgaag tttgccgcat tgccaatgaa gccaatacac
     2281 gtgttcggac cctgggcgtc gcattttctg gctgcacact acccggtgct caacagccgt
     2341 tatttcatgt cgaacacgga aaattggcag ttgggatggg tatccatggg gaaccgggtc
     2401 tctatcagca agatgggctt ccagcggatg atttagctgc gatgttagtc gagaagttgc
     2461 tacaagatat ctcaccggtt ggcgatttgc ctaaaggtac ccatatcagt gtgctattga
     2521 gcagcttggg gagtgtagcg caggaagaat tgtttgttgt ttatcgtaag gttgatcagt
     2581 tattgcacag tcgtggattg attatcgtcg aaccggttgt tgatcgcgtt attaccagct
     2641 ttgacatggc gggtatttcg ttgacgttgt tctggctaaa tgatgaactc gaaacttact
     2701 ggaaggctgc cgcagataca cccgctttta aacgaggcac tatttcatgc tttagaccag
     2761 agactgatgc tgcccttatc ccgcaggttg cagtggcc
//
LOCUS       DAOQKQ010000235         2795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_25079_length_2795_cov_6.625547, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000235 DAOQKQ010000000
VERSION     DAOQKQ010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25079_length_2795_cov_6.625547"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PKZ49_11800"
     CDS             <1..319
                     /locus_tag="PKZ49_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37290.1"
                     /translation="RIGDSGLLEMADLSQLDIVAEVYETDLHRVRVGQTGCISGAGFE
                     HTYLAEVRELGYLVRKNDINDTDPLSDRDNRIIEVRLTLEISAVENLQHQIFRQVKVK
                     ILP"
     gene            316..>1139
                     /locus_tag="PKZ49_11805"
     CDS             316..>1139
                     /locus_tag="PKZ49_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DevC protein"
                     /protein_id="HND37291.1"
                     /translation="MNYTNWLYFPMRLAWRQLIFDRTKLMVAICGVLFACVLVFMQLG
                     FRDALYASATSAPVKLDGDLFLMHKQSEAMWRPIEFKRSELMRALGNPAVADVYPLYL
                     GLAPFKNIETKVKRTLMVYGFDPDSTSFRIEAINNKREALKLKDTVLFDEYSRPEFGP
                     IGQLIEANRNLTEIKDYKVAIIGLFRMGVSFAADGNVVTSDLNFMRIFPKRSLDNIDV
                     GIIKLTPGSSQTSVKGELEKLLDESINIFTYQELLQFEKNYWANMAPIGFIFGFGT"
     assembly_gap    1140..1239
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1240..2002
                     /locus_tag="PKZ49_11810"
     CDS             <1240..2002
                     /locus_tag="PKZ49_11810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HND37292.1"
                     /translation="DAHSFNVFHRRINCDSETQRCRSGRYVLMQTAIDVQNLSFSFGS
                     GALVQPVLKEITISIQQGEIVLITGPSGSGKTTFLTIIGGLRQAFTGSVVVLGQQFIQ
                     SSEQVRVKVRQQIGFIFQQHNLLKSLTALQNVSMTLEMNNQLSEQQRQERAIAMLTAV
                     GLGDRIHYKPDQLSGGQRQRVSIARALVGQPRIVLADEPTASLDKQSGYEAVSILKKL
                     AKESQTTILLVTHDYRISDVADRILELEDGMIRNV"
     gene            2084..2701
                     /locus_tag="PKZ49_11815"
     CDS             2084..2701
                     /locus_tag="PKZ49_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37293.1"
                     /translation="MATTPILSSPQYLVWELFQALRRLCERVGESHIAQNESVSRQDA
                     ALCTILAVQCVEVFFNVYFKVLINEPDYAHANEKISNDLDNTMYSLEKKIKEWPRHAF
                     GKGLDLGGGAGQKFIALKERRNRLIHFTSSHVTFSMPGFAIYGMADTTDYHSLTAKSA
                     IDALQTAEAFLCEIFMLRGISPENLSSALHSWTGKPLIRKQSTAS"
BASE COUNT          803 a          554 c          580 g          758 t
ORIGIN      
        1 acgaatcggt gatagcggtt tattggaaat ggctgattta tcacagctcg atattgttgc
       61 agaagtctat gaaaccgatt tacatcgagt cagagtcggc cagaccggct gcatcagtgg
      121 agctggtttt gagcatactt atctcgctga agtcagagaa ttaggttatc tcgttcgcaa
      181 aaatgatatc aatgatacgg atcccctctc agaccgtgac aaccgtatca tcgaagttcg
      241 tctaacgctt gaaataagtg cagttgaaaa tttacaacat cagattttta gacaggtaaa
      301 agtaaaaatc ctgccatgaa ttacactaac tggctttatt ttccaatgcg tcttgcgtgg
      361 cgccagttaa tttttgatcg caccaaacta atggttgcga tctgtggtgt attgtttgcc
      421 tgtgtactgg ttttcatgca gttaggcttt cgagatgcac tctatgccag tgcaacatca
      481 gcaccggtca aactggatgg cgatttgttc ttaatgcata aacaaagcga agcgatgtgg
      541 cgacccatcg aatttaaacg tagcgagtta atgcgagcac taggtaatcc cgctgtcgct
      601 gatgtatatc cgctttatct aggattagcg cctttcaaaa atatcgagac taaggtcaag
      661 cgcacactca tggtatacgg ctttgatcca gactcaacat catttcgaat tgaagcgatc
      721 aacaacaaac gagaagcgtt aaaactaaaa gataccgtac tattcgacga atattcgcgc
      781 cctgaatttg gcccaattgg tcagttgata gaagcgaatc ggaatttgac tgaaatcaaa
      841 gattataaag tagcgattat cggcttgttt cggatgggtg tttcttttgc agcggatgga
      901 aatgtagtca ccagcgatct caattttatg cgcatttttc ccaaacgaag cttggataat
      961 atcgatgtgg gtattattaa attaacgcca ggttcatctc agacatctgt aaagggtgaa
     1021 cttgagaagc tgctagatga atcgattaac attttcacat accaagagtt attgcaattt
     1081 gagaagaatt actgggccaa tatggcaccc atcggtttta tttttggttt tggaacgatn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gatgctcatt ctttcaatgt
     1261 gtttcatcgc cggattaatt gcgattcgga aactcagaga tgccgatccg gcagatatgt
     1321 tctaatgcaa actgccatcg atgttcagaa tttaagcttt agttttggaa gcggtgcttt
     1381 agtacagccc gtattaaagg aaataacaat ctcgatccaa caaggtgaaa ttgttttaat
     1441 cacgggacca tccggctcag gaaaaaccac ctttttgact attattggtg gactcagaca
     1501 agctttcacc ggtagcgtag tagtactcgg gcagcaattt atccaaagta gtgaacaggt
     1561 tagagttaaa gttcgacaac aaatcggttt tatctttcag caacacaatt tacttaagtc
     1621 attaactgcg ctacaaaatg tcagcatgac gttggaaatg aataaccagc tctccgaaca
     1681 gcaacgacaa gagcgggcga tcgccatgct gactgcagtt ggtcttggtg acagaattca
     1741 ttacaaaccc gatcaacttt caggcgggca aagacagcgt gtctcgatcg cacgcgcgct
     1801 cgtgggacaa cccaggatag tattagccga tgaaccgact gcatcgctgg ataaacaaag
     1861 tggctatgaa gcggttagta tccttaagaa actagccaag gaatcacaaa caacgatact
     1921 cctagtcacc cacgattacc gaatttcaga tgtagcagat cgcattctcg aactcgagga
     1981 tggaatgatt aggaatgttt aataaatgct tttttatttt tcagagcaat tgttttttta
     2041 caaaattatt gaacaaaatg cgtcatagac tacgttaact ctcatggcta caactccaat
     2101 actatcatca cctcaatacc tagtctggga attgtttcag gctttaaggc ggctgtgcga
     2161 gcgcgttggg gaatctcata ttgcgcagaa tgaatcagta tcacgacaag atgctgctct
     2221 ctgcaccatt cttgctgtgc aatgtgtcga agtttttttc aacgtgtatt tcaaggttct
     2281 aatcaacgaa ccagactacg cgcatgccaa tgagaaaatc tctaatgatc ttgacaacac
     2341 aatgtatagc cttgaaaaaa aaatcaaaga atggccacgc catgctttcg gaaaagggtt
     2401 agatctcggt ggaggtgctg ggcaaaagtt tattgctctt aaagaacgtc gaaacaggct
     2461 tatacacttc actagctccc atgtaacatt ttctatgcca ggttttgcga tttacggaat
     2521 ggctgataca accgattatc actcactgac ggcaaaatcc gcgatagatg ctctgcaaac
     2581 ggcagaagca tttctctgtg agattttcat gctgagaggt atatctcccg agaacttatc
     2641 aagtgctttg cactcatgga caggcaagcc actgataaga aagcaaagca cggccagcta
     2701 aatgactaac aattgcttat tctcgctgta gatcaaaatt aaatatgcct ctaaactatt
     2761 ttcagtttaa tctgggtgtg actcaaacag gcatt
//
LOCUS       DAOQKQ010000236         2699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_26698_length_2699_cov_6.001513, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000236 DAOQKQ010000000
VERSION     DAOQKQ010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26698_length_2699_cov_6.001513"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            226..999
                     /locus_tag="PKZ49_11820"
     CDS             226..999
                     /locus_tag="PKZ49_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37294.1"
                     /translation="MKLRYFPSPNTAYTHRGRDYFIAGFTFFCVAVSLVIDAHANLLI
                     QNILGVIAWVFLVGLLVGENREIRVQVVIAVAFATAGEHFASIYMGGYTYRFENVPLY
                     VPPGHGMVYLTAVALARSGFFLKYARKIALFVVVTCGIWSLWGISGIPEHGDQVGAIL
                     YCVFLIYLFKGRSPMVYLAAFFITTWLEIIGTAAGTWKWATLEPIFQLSQGNPPSGVS
                     AWYCLVDAVAIGCAPIVLKGIRRMTDWAKSARSVLLNRR"
     gene            1036..2451
                     /locus_tag="PKZ49_11825"
     CDS             1036..2451
                     /locus_tag="PKZ49_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HND37295.1"
                     /translation="MIQVSSFDQLGLTQELLKAIADQGYTEPTPIQAKVIPPIIEGRD
                     IMGSAETGTGKTASFTLPMLYRLQPHANSSTSPAKHPTRALILAPTRELATQIYGSVK
                     EYGKYLQLRCAVIYGGVNIDPQIKELQAGVEILVATPGRLLDHIEQRTANLSKIEIII
                     LDEADRMLDMGFLPDIKRILALLPAQRQSLIFSATFNDEIRKLADKLLKQPLLIEVAK
                     RNAINESIAHVVHMVSTRYKFDLLLQLLKQADSNPILIFVRTKQGVDYLVKQLLQSEI
                     LVAAIHGDKNQQQRTQALSDFKEGKIKALVATDIAARGLDIENLNCVINFELPTTPED
                     YIHRIGRTGRAGCKGNAISLISASEKELLINIEKLLGTKLEIKEVPGFGVEETGDNRS
                     SNRLSQSPTRNKERQPQSNSYPKKRKNTPPIDPIFTQPYVPPVLTDKTNVADGITPRT
                     FKSTKKVIAALFAPPINQKLK"
BASE COUNT          837 a          491 c          542 g          829 t
ORIGIN      
        1 attccaagct agcgctaagg tagctaaaaa agacgataaa aaaggaaaag agtaacaatc
       61 atagctttaa ttaagagtaa taaaacatga ctgaagaagt aagctaactt cttcagttat
      121 aaaaaataaa agctttttat tagagataga aatgatcgtt tttctcttaa tcgtataact
      181 ggtttcaaac ccgctatcat agcgggtttt tattttgaga attagatgaa acttcgttat
      241 ttcccttcgc ctaatacagc ctatacccat agaggtagag attattttat tgcaggattt
      301 actttcttct gcgttgcagt aagcctagtc atcgatgctc acgcaaacct acttatacag
      361 aatattttgg gtgttatcgc ttgggttttt ttagtgggtc tattagtcgg tgagaatcgg
      421 gaaattagag ttcaagttgt gatagcagtt gcatttgcta cggcaggtga acattttgca
      481 tcgatttata tggggggtta tacctatcgt tttgagaatg tccctttgta cgttcctccc
      541 ggtcatggca tggtatatct aactgcagtc gcattagcac gttcaggttt ttttcttaaa
      601 tatgctcgga aaatcgcgtt gtttgtcgtg gtgacttgtg gcatatggtc tctctgggga
      661 attagtggta ttccagagca cggagatcaa gtgggagcaa ttttatactg cgttttttta
      721 atctatctct ttaaggggcg ttcgcccatg gtttatctag ccgcattttt tattacaaca
      781 tggctagaaa taattggtac cgcagctgga acttggaaat gggcaacgct tgaacccatt
      841 tttcagttat cacaaggtaa cccacccagt ggagtatcag catggtattg tctggtcgat
      901 gctgttgcga tcggatgtgc accaattgtg cttaaaggca ttcgtagaat gacagattgg
      961 gcaaaatcag cgagatcagt gttactgaat aggagatagc gcattcatca acattttttt
     1021 tacgattaac aaaatatgat tcaagtatca tcatttgatc aattaggatt aacacaagag
     1081 ttactaaaag cgattgctga tcagggctat actgaaccga cacccataca agccaaagtt
     1141 attccaccga ttattgaagg tagagatatc atggggagcg ctgaaactgg tacaggtaaa
     1201 acagccagct ttaccttacc gatgttatat cgattacaac ctcatgcaaa ttcaagtacc
     1261 tcaccagcta aacatccgac gcgtgcatta attcttgccc ctactcgtga actcgcaacc
     1321 cagatttatg gtagcgtgaa agaatacgga aaatatcttc agcttcggtg cgctgtcatc
     1381 tatggagggg taaatattga tcctcaaatc aaggaattac aggctggcgt tgagattcta
     1441 gtcgcaacgc cgggtcgctt attggatcat attgagcaga gaaccgctaa tttatcaaaa
     1501 attgaaataa tcatcttaga tgaagcggat cgtatgttag atatgggatt cttacccgat
     1561 atcaaacgga ttttggcttt attgcctgca cagcggcaaa gccttatttt ttcggcaacc
     1621 tttaatgatg aaattagaaa gcttgcagat aaattattaa aacaaccgtt actgattgaa
     1681 gtagcaaagc gtaatgcgat taacgaatcg attgcccatg tggtacacat ggtatcaact
     1741 cgatataagt tcgacttgct actacagtta ctgaagcagg ctgattcaaa tccaattttg
     1801 atctttgtga gaacaaagca aggtgtagat tatctcgtta aacaattact tcaatcggaa
     1861 attttggttg ctgcgattca tggtgataag aatcagcaac agcgtacaca agcactatca
     1921 gatttcaaag aagggaaaat aaaagcgctt gtcgcaaccg atattgctgc gcggggatta
     1981 gatattgaaa atttgaattg tgtcattaat tttgaattac cgaccacacc cgaagattat
     2041 attcatcgaa ttggacgtac tggaagagcc ggttgtaaag gtaatgccat ttcattaata
     2101 agcgcttctg agaaagaatt actcatcaat atagagaagc ttcttggaac aaaattagaa
     2161 atcaaggagg tacctggatt tggagtagaa gaaactggcg ataatcgatc atctaatcgg
     2221 ctatctcaat cgcctactag aaataaagaa aggcaacccc aatcaaattc ttatcctaag
     2281 aaacgaaaaa ataccccacc aattgaccct atttttaccc agccttatgt acctcccgta
     2341 ttaactgata aaacgaatgt agcggatgga atcactccta gaacttttaa atcaaccaag
     2401 aaagttattg ctgctttatt tgcacctcct atcaatcaga agttaaagta aaacaactca
     2461 tttgatttga gatttatcga tataaggctt ttctgtagtt ttagcgcctc caatttctaa
     2521 gttcttctac ttagtttttc ctcaattttt tcttaagcac tactttagta agaataaatc
     2581 agcattttgg tatttgttcc atagataaaa atagtcaatc gagtgttagt ctctgcatat
     2641 tcaaaaatgg gactaaagtt gatgggtcat gaattagagc agttttacgc gatattttt
//
LOCUS       DAOQKQ010000237         2675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_27108_length_2675_cov_5.464504, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000237 DAOQKQ010000000
VERSION     DAOQKQ010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27108_length_2675_cov_5.464504"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..1276
                     /locus_tag="PKZ49_11830"
                     /pseudo
     CDS             72..1276
                     /locus_tag="PKZ49_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168044.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            1372..2352
                     /locus_tag="PKZ49_11835"
     CDS             1372..2352
                     /locus_tag="PKZ49_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37296.1"
                     /translation="MKDLSSELSALVHLVKTNGYDHEFYVNLQKLLEDSPEILTRCFY
                     EAILQIGDLSVNPFSAKFGFETNAFCNEVAQEIWGDIPKIRINQLNGVKKDSENEFIS
                     LTDLIKENFPFYSNQIYMASYNSQNFSRFIDYIHTTDCYHLRDEITKICTAVYQGLPL
                     FKKTNDDYLQVADTDPDFVYIFMNGYYENLKTISRYPKWILGFHFKNSQLEIHKHFFD
                     GFNGSDEALKVRRTAFRKAIGEEDVFSSKPEPNKDTLTDIVDSHSTKLLKIQKKVLDR
                     FYGTQFDITNRDTWTNQSVIVDWLITEFNLSKREAEAIDLVTRPDAARGK"
     gene            2458..>2675
                     /locus_tag="PKZ49_11840"
     CDS             2458..>2675
                     /locus_tag="PKZ49_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HND37297.1"
                     /translation="MTETSKKSIPVALINFDDLPNSANVRQPVVQGLYGCSAASIWRG
                     VKNGRIPKPRKLSPRTTCWNVGELRRALA"
BASE COUNT          891 a          510 c          562 g          712 t
ORIGIN      
        1 tctagacata ccgcaaaaat aacggtactt ttggcggtat ttataaatac cgtcatgcaa
       61 ggtaccgtca aatgcttact gatacctcaa tacgtaacgc taaatcatca accaagcctt
      121 aaaaatatcc gatagtggcg gcttatattt acttatcaaa cctgcaggta aatattggcg
      181 catggattat cgctttgcag ataaacgcaa aactttagcg ttaggcgtat atccagctat
      241 tacactaact gccgctcgga agaaacgaga tgaagcacgt gagctacttg caaaagatgt
      301 tgatccggcc ttagtcaagg caattaaaaa acaagccaag aaacatgccc aagaaaacac
      361 ctttcaggct atagcgcttg aatggcacgc taaacagtcc actacctggg cagaatctac
      421 cacagccaat ataaaacgat gcttggaagc cgatatattc ccatggttag gtaatcgacc
      481 cattaaggaa atagccgcac ctgaactact ttcagtatta cgcaaaattg aaagccgtgg
      541 tgcccatgag aaagcgcaaa gaacacggga atatgttggt cgggtgttta gatatgccat
      601 acaaaccgga cgcgctgaac gtgacccaag tggtgatctt aaaggtgcgc ttactcccgt
      661 taaagcaaag caccatgcaa gtattactga cccacaaggc gttgctgaat tgatgaaagc
      721 tattgcagga tttaacggat catttgttac tcagtgtgcg ctgcaacttg ctccattagt
      781 atttgtaaga ccaggcgaat tacgtgaagc tgaatggagt gaaattaatt ttgataagtg
      841 tgaatggcgc attccaagtc acaaaatgaa aatgaatgag caacatattg ttccgctttc
      901 acgccaagca gttgaaatat tagaagctat acagccactt accggtaatg gtaaatatat
      961 ttttccaagc ataagaagta ccagcaggcc aatgagtgag aataccatca acgcagcact
     1021 aaggcgcata ggctatgata aaaatgaaat gacagggcac ggctttcgta gcatggccag
     1081 tacactgcta catgagcacg gttggccgca tgaagcgatt gagcggcaac tagcccatgc
     1141 ggaacgcaat aaagttgtgg ctgcgtataa ctacgccgaa cacctaccga aacggcgaga
     1201 aatgatgatc tggtgggcgg attatctgga tgaattggca gctggtgcca aggtggttaa
     1261 ttttcaaaaa acttgattga gtttaacagc gcctagctcg ggggagcgaa caccggtaac
     1321 ccttacccgg ttggcgtagt ttttaaatat agggtggtaa ataaggaagc catgaaagac
     1381 ttaagcagtg agttatcagc attggttcat ctagttaaaa cgaatggata tgatcatgaa
     1441 ttttatgtca atcttcagaa actccttgag gattctcccg aaatactaac gcggtgcttt
     1501 tatgaagcaa tacttcaaat tggagattta tctgtaaatc catttagtgc aaagtttggt
     1561 tttgaaacaa atgctttttg taatgaagta gcacaagaga tatggggtga tattcccaag
     1621 ataagaatta atcaattgaa tggcgtcaag aaagattcag aaaatgaatt catttctcta
     1681 accgatttaa ttaaagagaa ttttccgttc tattcaaatc aaatttatat ggctagttac
     1741 aattcacaaa atttctctag gttcatagac tatatccata caacggattg ctatcattta
     1801 agggatgaaa ttaccaaaat ttgtactgct gtttatcaag gattacctct tttcaaaaaa
     1861 acaaatgatg attacctgca agtggcagat accgatcctg attttgttta tatttttatg
     1921 aatggctatt acgaaaacct aaaaacaatt agtcgttatc caaaatggat acttggattt
     1981 catttcaaga atagtcagtt agagattcac aaacattttt ttgatggttt taacggaagt
     2041 gatgaagctt tgaaggtaag gcgaactgca tttagaaaag caatcgggga agaagatgtt
     2101 tttagtagta aaccagaacc aaataaagat acattaactg atattgtcga tagccatagc
     2161 acaaagttac tgaaaataca aaagaaagtt cttgatcgtt tttatggcac tcaatttgat
     2221 ataaccaatc gtgatacatg gacaaatcag agtgtaatag tggattggtt aataacagaa
     2281 ttcaatctgt ctaaacgtga agcggaggca atcgatttgg taacaagacc agatgcggca
     2341 agaggaaaat aaagctttta agtcaccctt aacccatcct tagagcagct actattgcca
     2401 taaaggcacc ctaatgccat atcttgttaa tcacctaatt tcataaaggt gattaacatg
     2461 actgaaactt caaagaaatc aattccagta gcattaataa atttcgacga tctccccaat
     2521 tcagcaaatg tcagacaacc tgttgttcaa ggcttgtacg gctgttcggc tgccagcatt
     2581 tggcgtggcg ttaagaatgg gcgcataccc aaaccgcgaa aattatcacc gcgcacgacg
     2641 tgctggaatg tgggcgaatt aaggcgcgca ctagc
//
LOCUS       DAOQKQ010000238         2645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_27676_length_2645_cov_5.659846, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000238 DAOQKQ010000000
VERSION     DAOQKQ010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27676_length_2645_cov_5.659846"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..1177)
                     /gene="holB"
                     /locus_tag="PKZ49_11845"
     CDS             complement(125..1177)
                     /gene="holB"
                     /locus_tag="PKZ49_11845"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HND37298.1"
                     /translation="MNQLYPWQQSVWPQIRNHVITKAQAFIFSGRKGIGQLEFSEYLA
                     QSLLCEQPTQAQFACGSCRSCQWFEQGTHPNFYRLEPEALLVESDLDATQALSDSKQS
                     SEDTNSKKKLSQQITIDQIRHLDQFLYLSGHQSGRKILLIHPADCMNVSAANAMLKRI
                     EEPPPQVLFFLVTDRLQALIPTIVSRCMRITLPHPDRLSVKNWLHQEGIEENSIDLSW
                     FGTSPQLVKRYLEAGYSAYYVELIEKISSPMQLDPITLAEKLHKQELSMIVSWLQKWC
                     YDLVSFQMLGRVYYHHRYEAAIKKIVATTEPKQLIDFWKRLAQFQQWSRHSLNARLFL
                     EELLFAYIKTCSRISN"
     gene            complement(1174..1806)
                     /gene="tmk"
                     /locus_tag="PKZ49_11850"
     CDS             complement(1174..1806)
                     /gene="tmk"
                     /locus_tag="PKZ49_11850"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HND37299.1"
                     /translation="MQKGKFITLEGIDGAGKSTHLKWIANYLASKSIRVLTTREPGGT
                     ELGENIRGLLLDHRQIMHAETEALLMFAARREHLDKLILPALNQGSWVISDRFTDASY
                     AYQGGGRGVSIDKLQILENWVQGQFQPDLTFYFDLSIEVARLRMQSIKIADRFEKEQN
                     DFFHRVRAAYITRAKQFPERMQVLDASRSIDDITHQLEKVLQPLLIEQKP"
     gene            1888..2475
                     /gene="ampD"
                     /locus_tag="PKZ49_11855"
     CDS             1888..2475
                     /gene="ampD"
                     /locus_tag="PKZ49_11855"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HND37300.1"
                     /translation="MKINTAGYLQNAIQIPSPNFDERPLGTTIRLLVIHNISLPPNVF
                     EGNAIIELFTNQLDCNTHPYYEGLRDLKVSSHFLIRRQGEIIQFVSCIKRAWHAGQSC
                     WQGEERCNDFSVGIELEGSDTTPFEAIQYAQLIALTKALFQAYPLEHIVGHADIAPTR
                     KTDPGPFFDWHGFRESIRAFIPTTVTTLPGNERSN"
     gene            complement(2444..>2645)
                     /locus_tag="PKZ49_11860"
     CDS             complement(2444..>2645)
                     /locus_tag="PKZ49_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HND37301.1"
                     /translation="QIIIRSQGSQTAVVWNPWEKIAKEMADLLDDDYLRLLCVETTNA
                     AADTILIQPGSQFSLIAHYQVK"
BASE COUNT          778 a          605 c          481 g          781 t
ORIGIN      
        1 caggcagcaa aaatagacgc tccagaaact gctgaaccgc taatccaaat tgaaactaag
       61 gattagactc gcaaccgaaa agggaataga tatattccct tttcaacaca tatattcgct
      121 taatctaatt tgaaattcga gaacaggttt ttatataagc gaatagcaat tcctcaagaa
      181 atagtcgcgc atttaaagaa tgccttgacc attgttgaaa ctgagctaat cgcttccaga
      241 aatcgataag ttgttttggc tcagttgttg ccacgatttt tttgatggct gcttcatacc
      301 gatgatgata gtaaacccta cctagcatct gaaaacttac taagtcataa caccattttt
      361 gtaaccaact aacgatcatt gataattcct gcttatggag cttctctgcc aatgtgatcg
      421 gatcaagctg catcggtgaa ctgattttct caatcaattc tacgtaatag gcagaataac
      481 ctgcttcgag atagcgtttc accaattgag gcgaagtacc aaaccaacta agatcgattg
      541 aattctcttc aatcccttct tggtgtaacc aattcttaac tgatagtcta tccgggtgag
      601 gcaaagtaat tctcatgcat cgactaacaa ttgttggaat taatgcttgt aaacgatcag
      661 tcaccagaaa aaacaaaacc tgcggaggag gttcttcaat tcgtttcagc atcgcattgg
      721 ctgctgaaac attcatacaa tcggctggat ggattaataa aatcttacgc cctgattgat
      781 gccctgataa ataaagaaat tgatcgagat ggcgaatttg atcaatcgta atttgctgac
      841 tcaatttctt ttttgaattt gtatcttcac ttgattgttt agaatcactg agtgcctgtg
      901 ttgcatcgag atcggattca accaacaatg cttcaggttc taaacgataa aaattaggat
      961 gagttccttg ttcaaaccat tgacaactcc tgcagctacc acaagcaaat tgcgcttggg
     1021 ttggttgttc gcataacagt gattgagcaa gatactcgga aaactcaagc tgacctattc
     1081 cttttcttcc actgaatatg aaagcttggg ccttggtaat cacatgattt cgaatttgag
     1141 gccaaactga ttgttgccaa ggataaagtt gattcatggt ttctgctcaa tcaacaaagg
     1201 ctgaagtact ttttctaact gatgagtgat gtcatcgatc gatcgactcg catcaagcac
     1261 ttgcattcgc tccggaaatt gctttgctct agttatgtat gctgctctta ctcgatgaaa
     1321 gaaatcattt tgttctttct caaaccggtc tgcgattttg atggattgca tccgaagtcg
     1381 tgccacctcg atcgataaat caaaataaaa cgtcaaatca ggttgaaatt gaccctgaac
     1441 ccaattttcc aaaatttgta acttatcgat tgaaacgcct cttccccccc cttggtaagc
     1501 ataactcgca tcggtaaatc gatctgatat cacccaactc ccctgattta aagcgggtag
     1561 aatcaattta tccaaatgtt cgcgtcgtgc tgcaaacatg agtaaagcct cggtttcagc
     1621 atgcattatc tgacgatgat ctaataacaa accacgaatg ttttcaccta actcggttcc
     1681 acccggctcg cgcgttgtca atacacgaat actcttactc gctagataat ttgctatcca
     1741 tttaagatga gtactcttcc ctgccccatc aataccttct aatgtaataa atttaccttt
     1801 ttgcatgcgc aaacgaacaa tattaaatta tgcttaactt gaactatgat atgacttgtt
     1861 ccatcaacat aaaacaatgc ttaatcgatg aaaatcaata cagcaggtta cctgcaaaac
     1921 gcaattcaga ttccatctcc taattttgat gagcgaccac tcggcaccac catacgttta
     1981 ttagtcattc acaatattag cctacccccc aatgtctttg aaggaaatgc catcatcgaa
     2041 ttgtttacca atcagctcga ttgcaatact catccttatt acgaaggatt acgcgacttg
     2101 aaggtttcaa gtcatttcct catccgtcgc caaggcgaaa tcatacagtt tgtttcctgc
     2161 attaaacggg cctggcatgc cggtcaatcg tgttggcaag gggaagaacg ctgcaatgat
     2221 ttctccgttg ggatcgaact agaaggatcc gatacgaccc cttttgaagc gattcaatat
     2281 gcccagttga ttgccttaac aaaagcatta tttcaggctt acccccttga acatattgtg
     2341 ggtcacgctg atattgcgcc aactcgcaaa accgatcccg gcccattctt tgactggcat
     2401 ggctttcgag aatccattcg agctttcatt ccgacaacgg tgactacttt acctggtaat
     2461 gagcgatcaa actaaactga ctcccaggtt gaatcaaaat agtatccgct gctgcattgg
     2521 tcgtttcaac gcaaagtaga cgtagatagt catcatccaa taaatcagcc atctccttag
     2581 cgatcttttc ccaaggattc cataccaccg cagtttggct cccttgcgac cgaatgatga
     2641 tttgc
//
LOCUS       DAOQKQ010000239         2550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_29618_length_2550_cov_6.133868, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000239 DAOQKQ010000000
VERSION     DAOQKQ010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29618_length_2550_cov_6.133868"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="PKZ49_11865"
     CDS             <1..618
                     /locus_tag="PKZ49_11865"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HND37302.1"
                     /translation="GKNMITLYGIEWSRAKYVLFTLAELGLDYQHIKINPFEDEKNTP
                     EYLKLNPLGQVPTLVDGDLVLTEAMAINFYLARKYGNGKLWINRLEAEALIYKWTFFA
                     ITQLEAACVDLILHRKVYDVKDRNIAIVREAEQKLVKPLRVLNEYLTGKDFLVADQFT
                     VADINLAGVLSYAQGGEFDFSPYSKVAQYLEVVLSRPTYKKTVAG"
     gene            complement(621..998)
                     /locus_tag="PKZ49_11870"
     CDS             complement(621..998)
                     /locus_tag="PKZ49_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HND37303.1"
                     /translation="MKVTTKAPIKVYYNAACPVCKAGIESQMEKMEACDVEWKNIHSN
                     TQFVDELNSDLEQVRKRLHVIDEHGVSHIGFDAFLILWQHSPHEEWKAKLLGLPIIKS
                     FGQFAYNLFAAGLYRWNRAKKHW"
     gene            complement(1009..1443)
                     /locus_tag="PKZ49_11875"
     CDS             complement(1009..1443)
                     /locus_tag="PKZ49_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HND37304.1"
                     /translation="MKFTTSIILGILIIGLTACSTTQPILYPNDHFLKMGKQVAERDI
                     EACRQLAESAGAEEQGTSKVGDVATSTAMGAGMGAASGAVGGAISGSAGSGSLIGAAS
                     GATFGLIRGLFGAARPAQPNQAYVNFVSRCLHDKGYEVTGWQ"
     gene            complement(1611..1967)
                     /locus_tag="PKZ49_11880"
     CDS             complement(1611..1967)
                     /locus_tag="PKZ49_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37305.1"
                     /translation="MTVDILYKLITLPREFSQLNTVSMYDLLKRTGYADLSKSISEDD
                     LYEKLLAHPEVITDWLEYSEDKRCAGWYFRSSNSDRYLVGYYGNGESFETEYSDKLKA
                     CAVFIKHELTNILKIS"
     gene            complement(1987..>2550)
                     /locus_tag="PKZ49_11885"
     CDS             complement(1987..>2550)
                     /locus_tag="PKZ49_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37306.1"
                     /translation="KSDREYLNFVEKVRKAFVFLSDLGFSEIEALPTLARYRKGNVEV
                     DIYHGRQSYEIGAGITAFGTRYSITEIIRANDLEVEKHFRYPITTTPEGVVAGLEELS
                     TLMKRYGRASLDGDPKYFQMLERQRELWSKEYSLEVLARQLRPKANQAFRQKDYVKAV
                     ELYSQFRGCLSLAEIKKLSIAKERCKD"
BASE COUNT          761 a          532 c          481 g          776 t
ORIGIN      
        1 gggaaaaata tgattacgct gtatggaatt gaatggtcac gcgcaaaata tgtattgttt
       61 accctcgctg aattgggcct tgattatcaa cacatcaaaa tcaatccatt tgaggatgag
      121 aaaaataccc ctgaatattt gaaattaaat cctctcggac aagttccgac gttggtagat
      181 ggagatctgg tattaactga agccatggcg atcaattttt atcttgcaag aaaatatggg
      241 aatgggaagc tatggattaa tcgattggaa gcggaagcat tgatctataa atggactttc
      301 tttgcaataa cacaactgga agcagcctgc gttgacctca ttctgcatcg aaaagtttat
      361 gatgtgaaag atagaaatat agcaatcgtg cgggaagcag aacagaagct tgttaaacca
      421 ttgagggtgc taaacgaata tcttactggt aaagattttc tggtggctga tcaatttacg
      481 gttgctgaca ttaatctcgc tggagtttta tcctacgcac aagggggaga attcgatttt
      541 tcaccctata gcaaagtcgc acagtactta gaagtcgttt tatcgcgacc tacttataaa
      601 aaaacggtag caggctagta ttaccaatgc tttttagcac gattccagcg ataaagcccg
      661 gcagcaaata aattataggc aaactgtcca aatgatttga tgatgggcag ccccagcaat
      721 ttggctttcc attcttcatg cggagaatgt tgccacaaga tcaagaacgc atcgaaacca
      781 atatgcgata caccgtgctc atcgatcaca tggagacgtt tgcgaacttg ctcaagatct
      841 gagttaagtt catcaacaaa ttgcgtgttg gaatgaatat ttttccattc aacatcgcat
      901 gcctccattt tttccatctg actttcaatt ccggccttac aaacaggaca tgcagcgttg
      961 taatacactt tgatgggagc cttagttgtc actttcactg aattttacct actgccagcc
     1021 tgttacttca tatcctttgt cgtgtaggca gcgcgaaaca aaattcacat aggcttgatt
     1081 aggctgtgca ggccgtgccg caccaaagag tcctctgatc agtccaaatg tggcgccgct
     1141 tgctgcgcca atcaatgaac ccgagcccgc tgaccctgaa atggcccccc ctactgcacc
     1201 actcgcagcc cccatgccag cgcccattgc agtactggtt gcaacatctc caaccttact
     1261 cgtcccttgt tcttcagcgc ctgctgattc agctaattgt ctacatgctt caatatctcg
     1321 ctctgcaact tgcttgccca tttttaagaa atgatcgtta ggatatagaa tcggttgtgt
     1381 ggtcgagcaa gcagtcaatc caataatcaa aatccctaaa attatcgacg ttgtaaattt
     1441 catggcaatt aaccttgttc aaaggaattg ggaagtatag cgtgaaaaag attagaagta
     1501 gcattaccct taatgctaga aaatataata gaaattttat aaatgaggaa ttgatacaag
     1561 gcaatgcgat tttataactt tacttcaatt aaagttattt aactgagctg ctaacttatt
     1621 ttaaggatat tggttaattc atgcttaata aagacagcgc aggcttttaa tttatcactg
     1681 tattcagttt caaaactttc tccattaccg taatatccaa ctaagtatct atctgaatta
     1741 cttgaacgga agtaccaacc cgcacatctt ttatcttcgg aatattcgag ccaatcagta
     1801 atgacttcag gatgagcaag taatttctca tataaatcat cttcagaaat agattttgag
     1861 agatctgcat atccggtcct tttaaggaga tcatacattg atactgtatt tagctgagaa
     1921 aattcgcgcg gaagcgttat aagtttatat aaaatatcta ctgtcatatc attttcatat
     1981 acgaaattag tctttgcagc gttcttttgc gatacttaat tttttaatct cagcaagact
     2041 taaacaccct ctgaattgag aatatagttc aaccgctttg acgtaatctt tttgtctaaa
     2101 cgcttggttc gcttttggtc gcaattgtcg agctaaaact tcaagactat attccttaga
     2161 ccacaactcg cgctgcctct caagcatttg gaagtatttt ggatcgccat cgagagatgc
     2221 acgaccatat cgtttcatca atgtacttaa ttcttcaaga ccagcaacaa ctccttcagg
     2281 tgtagttgtt attgggtagc gaaaatgctt ttctacttcg agatcattag ctcggataat
     2341 ttccgtaata gaatatcgag tcccaaatgc agtaatacct gctccaattt catatgattg
     2401 ccgaccatga tatatatcta cttcaacgtt acctttgcgg tagcgcgcga gtgttggtaa
     2461 agcttcaatt tcagagaatc ctaaatcact cagaaataca aaggcttttc tgactttttc
     2521 cacaaaattt agatactctc gatctgattt
//
LOCUS       DAOQKQ010000240         2450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_31876_length_2450_cov_4.105219, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000240 DAOQKQ010000000
VERSION     DAOQKQ010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31876_length_2450_cov_4.105219"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..233)
                     /locus_tag="PKZ49_11890"
     CDS             complement(72..233)
                     /locus_tag="PKZ49_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37307.1"
                     /translation="MPIPRASLTSLKITCVPSYWHCLVKVDDMGGHMACMLEFFFDAQ
                     IKGESQPYK"
     gene            226..534
                     /locus_tag="PKZ49_11895"
     CDS             226..534
                     /locus_tag="PKZ49_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37308.1"
                     /translation="MGISKSFKAASTVFPAGNDHYHLACACQEFGHQVVRTAIATDLV
                     VTHGLSILDASHVAGCSLDIMLAMQRGEAPMKQMREKLVAEQATKASKDDCFDIICVS
                     "
     gene            546..2138
                     /locus_tag="PKZ49_11900"
     CDS             546..2138
                     /locus_tag="PKZ49_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37309.1"
                     /translation="MIDLDGVVIDRELTRDLIIQEVHAVLGSDVDPFKSLYLRGINSA
                     FTQMCFKHRTNPIPFPGRRLPGTYLELIYHGCLSEEEKLKAFPKSYMLGSRVEISKED
                     RSSTAILRYMEEEFGYGEFNEDRIREVQAGIFKITLMPWESYTLVEPSIAWKVACQLL
                     RLQHYGDKHLLSGLAPIGYGNNHEPNYWNVGYAPQVINHEDRSVRALAIQELQASLTF
                     EMPDEIRRNLVSGIDLLMRTLSKIGIPFRYLLGHGFEYANNALAEVCDKWKGFFEQSV
                     IDGTTVEHIPERLDVGLYKAVAAWQLDSVSGSNSIIAKQAMEQAVVIVNLSEVSDDIL
                     NADYNREGVSYLLQDAGVNMELIGKDRVFELVEALLQWPVVTTNPLYKDKYYQPTQLE
                     GVAAALSILDVMERQVSIKCYKPKVGGSQRSVLQAVKEVTRGKISWNKASPEIQEFWR
                     LRVGFIKYACVDDPENVRFTNHWHNELAKMVSASLRILTTEDCIRVPLTCLMPLYDRV
                     FDEKEFVGQDEELIYLLGLFRS"
BASE COUNT          662 a          469 c          626 g          693 t
ORIGIN      
        1 aagcatttga acagtttaag atggcggctt aatttatccc aagtccagtc atacggtaaa
       61 ctcaacacat atcacttata cggctgactt tctcctttaa tttgtgcgtc aaaaaaaaat
      121 tccagcatac aggccatatg tccgcccata tcatcgacct tgaccaagca gtgccaatac
      181 gagggcacgc aggtaatttt taaacttgtt aaggaggctc taggtatggg catttcgaaa
      241 tccttcaaag ctgcatcgac ggtattcccg gcaggcaacg atcattacca tttggcatgc
      301 gcctgccaag agtttggcca tcaggttgtg cgtactgcca ttgctactga tttggttgtg
      361 actcatggtc tgtcaattct tgatgcatcg cacgtagcgg gctgcagcct tgatatcatg
      421 ctggcgatgc agcgtggcga agcaccgatg aagcagatgc gggagaagct ggtcgctgag
      481 caggcgacta aggcttctaa agatgattgc ttcgacatta tttgtgtttc gtaagggaga
      541 ctacaatgat tgatctggat ggtgtagtta ttgatcgcga gctgacccgt gatcttatta
      601 ttcaggaagt tcatgctgta ctgggatcag atgttgaccc ttttaaaagc ctttacttgc
      661 gtggcatcaa tagcgcgttc acgcagatgt gctttaagca tcgtaccaat ccaattccat
      721 ttccagggcg ccgcttgcct ggaacatact tggagttgat ctaccatggt tgcctttcag
      781 aagaggagaa attgaaggct tttcctaaat cgtacatgtt gggtagccgt gttgagatta
      841 gcaaagaaga taggtcgagt acggctattc tcaggtatat ggaagaagag ttcggctatg
      901 gtgagttcaa cgaagatcgc atccgagagg tgcaggcggg aatttttaag attacgctaa
      961 tgccttggga aagttacacg cttgtagagc cttcaatcgc atggaaagtt gcttgccaac
     1021 tccttcgcct gcaacattat ggcgataaac acctgctgtc aggcttggca cctattggct
     1081 atggcaacaa tcatgagccc aactattgga atgtcggtta tgctcctcag gttataaatc
     1141 acgaagatcg ttccgtacgt gctttagcga ttcaagagtt acaggcatcg ttaacttttg
     1201 aaatgcctga cgagattcgc cgtaatttgg tatccggcat tgatttgcta atgcggacac
     1261 tgagtaaaat tggtattcct ttccggtatt tgttagggca tggcttcgaa tatgcgaata
     1321 atgctttggc tgaagtttgt gacaaatgga aagggttttt cgagcaaagc gtgattgatg
     1381 ggactactgt agagcacatc cctgaacgtt tagatgttgg gctctataag gcggtagctg
     1441 cgtggcaact ggatagtgtg agtggtagta atagcattat agcgaagcaa gctatggagc
     1501 aagcggtagt aatagttaat ctttctgaag tgtcagatga tattctcaat gctgattaca
     1561 accgagaggg cgtgagttat ttgctgcaag acgcaggtgt taatatggag ctgatcggca
     1621 aagatcgtgt atttgagctt gtcgaggcat tgttacaatg gccagtagta accacgaacc
     1681 cgttgtataa ggataagtat taccaaccga cgcaattgga gggggttgcc gctgccctgt
     1741 caatactcga tgtgatggag cgacaagtca gtatcaaatg ctacaagcct aaggttggcg
     1801 gtagtcaacg atcagtgtta caggctgtaa aagaggtgac aagaggtaaa atctcttgga
     1861 ataaggcttc ccctgaaatt caagagttct ggcgcttgcg agtgggtttt ataaaatatg
     1921 cttgcgttga tgatccagaa aatgtcaggt tcactaatca ctggcataat gagcttgcaa
     1981 aaatggtatc agcatctttg cgcatcttga cgacagaaga ctgcattcga gtaccgttga
     2041 cctgtctgat gccgctctat gatcgtgtat ttgatgaaaa agagtttgtg gggcaggatg
     2101 aagagcttat ttacttactc ggtctctttc gcagttagta tttgaggata agtgcaagcc
     2161 gtaaggcagc taataaggac agcagaaata gaaacgggtc gcaaaagcga cccgtataaa
     2221 tgggggtctg aggcgcagtg gaacggaaaa ccgagttaag aggccaatat agatggtttt
     2281 gttggacaaa tcaaatttga gttaggtttt tccgtaatgc cttattctac agaatcgttc
     2341 attactttgc tatttgggct tataacccca aaatagtgat aacactattt tctttgtttg
     2401 cggaaattga gcgagactta attagtttac gtactaaaga agcgttagct
//
LOCUS       DAOQKQ010000241         2408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_32864_length_2408_cov_6.393965, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000241 DAOQKQ010000000
VERSION     DAOQKQ010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32864_length_2408_cov_6.393965"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(398..1042)
                     /locus_tag="PKZ49_11905"
     CDS             complement(398..1042)
                     /locus_tag="PKZ49_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37310.1"
                     /translation="MRMQIVLLAASLGFATVSLAKPSFQLDEIDRDGNTLIIKFSGNR
                     SGNIVQDIENIHAWYNGKELGVGLPHGLYNYFWQPISGRWGADGAQISFDGRANNFLF
                     TDLSFTPINLPAGQFLTNGDYVYDVSKFFGNALPLPQDDPAAYSEVTVRYPTLGTPST
                     SFAFDYHDNVTYSLSEIQIPVPGSISLIACGVLGLFLLHRRSRDKRNNLIRMIF"
     gene            complement(1710..>2408)
                     /locus_tag="PKZ49_11910"
     CDS             complement(1710..>2408)
                     /locus_tag="PKZ49_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HND37311.1"
                     /translation="AAGLTEAEARSRLWFIDRQGLVTSNREEIKPLIKPFAHPCAPAT
                     FLEAINRIRPDVLIGATGMAGTFNQDIITAMATVNERPVIIALSNPTSHTECTAEEAY
                     RWSNGRAIFASGSPFEPVTYAGKLLKPGQGNNAYIFPGVGLGVTISKARLVTEPMFLA
                     AAHALSAQILPEEIAAGSIYPSINRVRPVSLAIAVAVCQVAKQAGLTEETLPETDLAS
                     YIAAKMYDPQYRAG"
BASE COUNT          664 a          515 c          565 g          664 t
ORIGIN      
        1 gtcaccgtaa ttgcagctgg caaacgcgac cggaatgaaa tctacgacat cgccctctcg
       61 cgattgcgtg aaaaggcatg agtcataaca tcgaagtaat tcatttctgt cacaaaaatc
      121 acttagtaat gctttctaga ttgaatatta tgctgcgatc gattggaaat cgtatagcgt
      181 gaaagacgtc ccttaacctt ggaaaatgat cagtcagtat acaattagtg acactaatta
      241 catctgacct cactaaccag agcagttgag aggaatcaat cgattccgtg gtactccgat
      301 gccaaaacat ttcctacgcc tcaatattta atactgattt gttcaagtac tattagctca
      361 gttcaaaaaa acaacgtaag gaatttttaa ttgaacctta aaatatcatg cgaataaggt
      421 tgttcctttt atcgcgagat ctcctatgca agagaaatag tccgagcacc ccgcacgcga
      481 tcagagaaat ggaaccaggc accggtattt gtatttccga caggctgtaa gttacgttgt
      541 catgatagtc aaaagcgaat gatgtactag gcgtccctag tgttggatac cttactgtta
      601 cttcagagta agcagcaggg tcatcctggg gtaagggaag tgcattgccg aagaacttgc
      661 ttacatcgta aacataatct ccattggtta aaaattgacc agcaggtaaa ttgatgggcg
      721 tgaaggatag atcggtgaaa agaaaattat tggctctacc atcgaaggag atctgtgcac
      781 catctgcgcc ccatcgaccg gaaatgggct gccaaaaata attatagagg ccgtgtggta
      841 gtccgactcc aagttctttt ccgttatacc aagcatgtat gttctctata tcctggacaa
      901 tgtttccgga gcgattgccg ctgaatttta tgattaaggt attaccatct ctatctattt
      961 catctagctg aaaagacggc ttagccaagg atacggttgc aaagccaagt gacgcggcta
     1021 gaagtactat ttgcatgcgc atttaaagtc tcctttgtgg cgtataggaa aaataatcgt
     1081 tttcttttat aagaaccaaa tctactagta agggattggg ttgtcaaact gaccagaaaa
     1141 tcatccggaa ttcacaaaat ttgtctttaa tcagaggcaa aaagtgctgg ttttgtccct
     1201 ttttttgccg ttcacgctaa tggcgttcaa gagtagacgt cagcgtattc aggaaatgca
     1261 ctgacgtagc gaaactctgg aggataagcc gaaatagtca agaatctttt gattgctgct
     1321 cttttcaaga tggtggcgat gattttcagg actccactgc aatacaagca gcactcaata
     1381 tcggataccc gttcttttag cgaatgtgtc taattgatac gtgccgagac agagggatat
     1441 ggcacatcgt cattcgtatc agatacattg tttttcatgt tgcctggaat aatctcagag
     1501 tgaagcttcc tgcttggcgc gagcatgtca tggaaatgaa taaggttgga ccttggccag
     1561 agaactaaag cagctagttg ttatatgttt tcgaatcact tgccaagagc ggttaacagt
     1621 aaactacttg agcatgagtg tggaagcaga cggcatgatt gagaatttgt taagcactcg
     1681 gctaatcatg agtgaccgag cggcaaatcc taacccgcgc gatactgtgg atcatacatt
     1741 tttgcagcga tgtaactcgc taaatctgtt tcaggtagcg tttcttctgt taaacccgct
     1801 tgttttgcga cttggcaaac tgcaactgca atcgcgagtg aaacggggcg cacgcgattg
     1861 atcgaagggt aaatcgatcc ggctgcgatt tcttccggta aaatctgcgc tgagagtgcg
     1921 tgggcggcag ctaagaacat cggttctgtc actaatcgtg ctttgctgat ggttacccca
     1981 aggccaaccc ctggaaaaat gtaagcattg ttgccttgac ccggcttgag cagttttcct
     2041 gcgtatgtca ccggttcgaa tgggctccca ctggcaaaaa tcgctctacc attggaccat
     2101 cgataggctt cttccgcagt gcattcagta tgagacgttg ggtttgacag cgcaatgatc
     2161 acgggacgtt cgttgacggt tgccatcgca gtgatgatgt cttgattgaa tgtcccagcc
     2221 atgcccgtgg cgccgatgag cacatcagga cgaattcggt ttatcgcctc cagaaaggtc
     2281 gcgggagcac agggatgtgc aaagggtttg ataagcggtt tgatttcttc tcgattcgat
     2341 gtaactaacc cttgccgatc aataaaccat aacctggatc gtgcttctgc ttcagtcagt
     2401 cccgctgc
//
LOCUS       DAOQKQ010000242         2386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_33431_length_2386_cov_6.009009, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000242 DAOQKQ010000000
VERSION     DAOQKQ010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33431_length_2386_cov_6.009009"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PKZ49_11915"
     CDS             <1..186
                     /locus_tag="PKZ49_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37312.1"
                     /translation="PIIGFPIKFPTPIETGMVVGKSAIAVGILKGHVSTKGIGVVPAP
                     GDGVALIHNQPWSVEVV"
     gene            complement(422..766)
                     /locus_tag="PKZ49_11920"
     CDS             complement(422..766)
                     /locus_tag="PKZ49_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37313.1"
                     /translation="MRRLAEMLEIFLKKCFPTRVTFDDGSYIEHLNYEAILYAEKDGH
                     QMEVVWYFNSHFGSARLLYADDIDYWNSPYELETLSDQKKKEIQQKIVSYCQKRKISI
                     EIIPVGSKPPSL"
     gene            complement(773..>2386)
                     /locus_tag="PKZ49_11925"
     CDS             complement(773..>2386)
                     /locus_tag="PKZ49_11925"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HND37314.1"
                     /translation="QTGKLTQTTYPSGAQISTLYGIDGRPNEILLNGTTLIRDITYQP
                     FGTAKSWVWWNNQTYTRQFDTNGRLTQYPNGNTTRTIHFDEAGRIAQYLHIDTPVLNR
                     NFTHDSLDRLTSEVGSTSMALWTYDANGNRITSQPGSQLYTYDYLPNSNRLLSVSGSA
                     VKSYSYDLAGNIIHDGSTSYTYNTGGRLSRVDRASKTNWYFYNAFGERVIKAGAWLVN
                     GPYRFVYDSDGKLIGEYDKNGPRQETVWLDDTPIAVIKKNSTGEFTVYQIHADHLNTP
                     RAILDSQNRVVWQWHSGAFGETLPEEDPDGDGAKFEYNLRFPGQYWDKETNLHYNYFR
                     DYDPETGRYISPDPIGLMGGMNVWGYVGQRPMGFVDSYGLWAIGDPLSQGIVNAIAGF
                     GDGISSFVTFGMYSTIDARKILGINGGVDYCSASYSSGKYAGYTWGAGTLWVAGLNGG
                     ANSVFWAGKGASIDAAAIGTTIAKTPIGAILNGLGVKNKLVWNLASATFAANASGHAT
                     VVIRHVAPNSSWQIERAILNWRGIPIHIR"
BASE COUNT          654 a          558 c          481 g          693 t
ORIGIN      
        1 ccaataattg gttttcccat caaatttcca acccctatcg aaacggggat ggtagtcgga
       61 aagagtgcca ttgccgttgg aatcctcaaa gggcatgtta gcaccaaagg cattggtgtt
      121 gttccagcgc caggtgatgg tgttgctttg attcacaatc agccgtggag tgttgaggtg
      181 gtctaacttt ccacatctca cagcgctgat acctcgcccc ttggggcgga gtcgttcatt
      241 taacgcgttg atccagggta ttggttttgt atgtgtgcgt tttgctattg ttgaccgtgg
      301 ttgcaagttt ttccgtgaag ttccaggtga aggtagtagt accattgctc aacagattgt
      361 cggcggcatc aaaactgtaa tacacgatat caagacctgg tgcctttagt gctttagaac
      421 atcacaagct tggtggtttc gatccaactg ggataatttc gattgaaatt ttccgtttct
      481 gacaatagct aacaattttt tgttgaattt cttttttctt ttgatcagat agagtctcaa
      541 gctcataagg ggaattccag taatcaatat catctgcgta aagaagtcgt gcacttccaa
      601 agtgactatt gaaataccaa accacttcca tttgatgtcc atctttttca gcataaagaa
      661 tggcttcata attcaaatgt tcgatatatg atccatcatc aaatgtaacc ctggttggaa
      721 agcatttctt taggaaaatt tccagcattt cagctaatct tctcattatt aatcacctta
      781 tgtgaatggg aataccgcgc caattcaata ttgctcgctc aatttgccag gaagagtttg
      841 gagccacatg gcgaattaca actgttgcat gaccggaagc attagcagca aatgtcgcag
      901 aagctagatt ccaaactaac ttgttcttga ctccgagtcc atttaaaatg gctccgattg
      961 gtgtttttgc aatagtagtc cctattgctg cagcatcaat gctagcccct ttacccgccc
     1021 agaataccga gttagcacca ccattaagac cagcaaccca cagtgttcca gcgccccaag
     1081 tgtaccctgc atacttaccg ctagaatatg acgctgaaca ataatctact ccaccattga
     1141 tgcctaaaat tttgcgtgca tcgatagtcg agtacattcc aaaggttaca aaacttgaaa
     1201 ttccatctcc aaaacccgct atagcattga caattccttg cgatagagga tccccaatag
     1261 cccacaaacc atacgaatcg acaaaaccca ttggcctctg tcccacatac ccccaaacat
     1321 tcattcctcc catcaaccca attggatcag gcgagatgta ccttcctgtt tccggatcat
     1381 aatcacgaaa ataattgtag tgaagattgg tttccttatc ccaatattgc cccggaaatc
     1441 gaagattgta ttcaaatttg gccccatccc catcaggatc ttcttcgggt aaggtttcac
     1501 caaaagcgcc gctatgccac tgccaaacga cacgattttg tgaatcaaga attgcacgcg
     1561 gggtatttaa atgatcagcg tgaatttgat aaaccgtaaa ttcacctgtg ctgtttttct
     1621 taatgacagc gattggcgta tcatcgagcc atacggtttc ctgccttggc ccatttttat
     1681 catattcacc gattaatttt ccatctgaat cgtatacaaa tcgataaggt ccattgacca
     1741 accatgcacc ggctttgatc acacgttcac caaacgcatt ataaaaatac caattcgttt
     1801 tgcttgcacg atcaactcgg gacaggcgcc cccccgtgtt ataggtatag ctggttgagc
     1861 catcatgaat aatatttccc gccaaatcat acgaatagga tttaacggct gacccgctaa
     1921 cacttagcag ccggttacta ttgggaaggt aatcataggt ataaagctga ctacccggtt
     1981 gactggtgat acggttgcca ttcgcgtcat aagtccatag cgccatgctg gtactaccaa
     2041 cttcactcgt aagacggtcg aggctatcat gcgtgaaatt acgattaagc acaggagtat
     2101 cgatgtgcaa atattgagca atacggcctg cttcatcaaa atggatagtg cgtgtggtat
     2161 tcccattcgg atattgagtt aatcgcccat tagtatcaaa ttgacgtgta taggtttgat
     2221 tattccacca gacccaactt ttagctgttc caaacggttg ataagtaata tcgcgtatca
     2281 gcgtcgtgcc attcaataga atttcattcg gtcgcccatc gattccatat agcgtcgata
     2341 tctgagcgcc agagggataa gtggtttgtg taagcttacc ggtttg
//
LOCUS       DAOQKQ010000243         2382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_33517_length_2382_cov_5.698754, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000243 DAOQKQ010000000
VERSION     DAOQKQ010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33517_length_2382_cov_5.698754"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..>2382)
                     /gene="leuS"
                     /locus_tag="PKZ49_11930"
     CDS             complement(139..>2382)
                     /gene="leuS"
                     /locus_tag="PKZ49_11930"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HND37315.1"
                     /translation="WTREIATCDPHYYRWNQWLFLRMLEQGIAYKKTQVVNWDPIDQT
                     VLANEQVIDGRGWRTGALIEKREIPGYYLAITRYADELLDTLDTLPGWPERVRTMQAN
                     WIGKSYGVDITFPVSKPDEFSQDLKVFTTRADTLMGVTYLAIAAEHPLALHAAKNNLS
                     LAKFLEECKQGSTIEAELATQAKKGMDTGFTALHPITGESIPIWVANYVLMSYGEGAV
                     MAVPAHDERDFEFATQYQLPIKIVIRPQNEDELLPPNQAFIDYGITINSDEFSGLQSQ
                     AAIDAIAAHLELKQLGKKRTRYRLRDWGISRQRYWGCPIPLIHCDRCGTVPVPDDQLP
                     VVLPENLVPDGSGNPLAKSSEFSVCSCPSCGQSARRETDTMDTFVDSSWYFIRYACAD
                     QNTCMSDSRASYWLPVDQYIGGIEHAILHLLYSRFWSKVMRDLGLVHFNEPFTNLLTQ
                     GMVLNEIFFRKSATGRINYFNPTEIDIEVDAQGKRTKMMLRADTLPVESGGIGTMSKS
                     KNNGVDPQELVDSYGADTARLFMMFSSPPTQTLGWSETGVEGAYRFLRRLWRQVYLHL
                     QHPSQPDAPVIDIKDWSPALKTLRGQLHQTIAKVTDDLERRHTFNTAIASIMELMNEL
                     AKLTDTESANRQLMQEALEKVVLLLSPIVPHICHTLWQELKPGTALLEQSWPEVDHTA
                     LVQDTIDLVIQVNGKLRGQMNVPKDTDRATIEQLAINLENVQKIVAGNAIKKIIVVPG
                     RLVNIVY"
BASE COUNT          618 a          526 c          562 g          676 t
ORIGIN      
        1 attggataca agtcctcgca actgaaatcc acaggctgag agcgcgagta ctatgctgaa
       61 gataaaaaat atacgagaaa cgatcatgcg gttgtacctt ctttcaaaat tttaagtaaa
      121 tcaggctaca tcgagcgact aatatacgat attaacgagt cttccaggta cgacaattat
      181 ctttttgatg gcatttcccg caactatttt ttgcacattc tcaagattaa ttgccaattg
      241 ttcaatcgta gcacgatcgg tatctttagg aacattcatc tgtccccgta attttccatt
      301 gacttggatt accagatcaa tggtatcttg aactaatgcg gtatgatcga cctcaggcca
      361 actttgctcg agtaacgctg taccaggttt gagttcctgc cataatgtat ggcagatgtg
      421 aggaacaata ggtgatagca atagaaccac tttttccaac gcttcttgca tcaattgtcg
      481 attcgctgac tcagtatcag tgagtttcgc taattcattc attaattcca taatggacgc
      541 aatggcggta ttgaacgtat gccgccgttc cagatcatcg gtgactttag ctatagtttg
      601 atgcaattgc ccgcgaagcg ttttgagggc tggtgaccaa tctttgatat cgataaccgg
      661 cgcatcgggt tgggagggat gctgtagatg gagataaacc tgtcgccata atcgcctgag
      721 aaaacgataa gccccttcta cgcctgtttc tgaccatccc agtgtttgtg tcgggggact
      781 cgaaaacatc atgaacagtc gagccgtatc cgcaccatag gaatcgacta gttcttgggg
      841 atcaacgcca ttgtttttcg atttcgacat ggtaccgatt ccaccggact cgactggcag
      901 agtatcggca cgtaacatca ttttggttcg tttaccttgc gcgtcgactt ctatatcaat
      961 ctcagtcggg ttgaaataat tgatgcgtcc cgttgctgat ttgcggaaga agatttcatt
     1021 taataccatt ccttgagtta atagattagt aaacggttca ttgaagtgaa ctaatccaag
     1081 atctcgcatg actttgctcc agaatcgtga gtagagcaaa tgcagaatgg cgtgctcaat
     1141 gcccccaata tactgatcga caggcaacca atagctggct cgcgaatccg acatacaggt
     1201 attctgatcg gcgcatgcat agcgtatgaa ataccaagat gagtcgacaa aggtatccat
     1261 tgtgtctgtt tcccgccgag cagactgtcc acaggacggg cacgagcaga cactaaattc
     1321 gcttgattta gcgagtgggt tgcctgaccc atcgggaact agattttctg gcagtacaac
     1381 tggaagttga tcatcaggaa ctggaacggt accacagcga tcgcagtgga tgaggggaat
     1441 agggcagccc caataacgtt gccgtgaaat tccccaatcg cgtaggcggt aacgtgtccg
     1501 ttttttaccg agttgtttga gttctagatg agctgcgatt gcatcaattg cagcttgaga
     1561 ttgcaaccca gaaaattcgt ctgaattgat ggtaatacca taatcaataa atgcttgatt
     1621 gggtggaagc aattcatcct cattttgggg tcgaataacg atcttgatgg gaagttgata
     1681 ttgtgttgca aattcgaagt cgcgttcatc atgtgcaggt actgccatca cagcaccttc
     1741 tccatagctc attagcacat agtttgcgac ccagatggga atactttccc ccgtaatcgg
     1801 atggagcgcg gtgaagcctg tatccatgcc tttcttcgct tgagtagcca attctgcttc
     1861 gatggttgag ccttgtttgc attcttcaag gaatttggca aggctaagat tatttttagc
     1921 tgcgtgtaat gcgagggggt gctctgctgc aatcgccaaa taggtaacac ccatgagcgt
     1981 atctgcgcgt gtcgtgaaaa ccttcaaatc ctgggaaaat tcatctggtt tgctaactgg
     2041 aaaagtgata tctacaccat aacttttacc aatccagttg gcttgcatcg ttctgacacg
     2101 ttctggccag cccggtagcg tatcgagcgt gtctaataat tcgtcagcgt aacgggtaat
     2161 tgcgaggtaa taacccggga tttcacgttt ttcgatgagt gcaccggttc tccagccacg
     2221 gccatcgatg acttgctcgt tagcgagtac cgtttggtct atgggatccc agttgactac
     2281 ttgggttttt ttgtaggcaa tgccttgttc gagcatgcgt aaaaaaagcc attgattcca
     2341 gcgataataa tggggatcac aggtagcaat ttcacgcgtc ca
//
LOCUS       DAOQKQ010000244         2374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_33704_length_2374_cov_6.447607, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000244 DAOQKQ010000000
VERSION     DAOQKQ010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33704_length_2374_cov_6.447607"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="PKZ49_11935"
     CDS             complement(<1..674)
                     /locus_tag="PKZ49_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HND37316.1"
                     /translation="MALTEQHIEETLKTVIDQTTGKDYVSSKAVENIKIVDNHVSLDI
                     ALGYPAKTMLESIRQQISEALKSIAGVSTVTVNVFSKIIPHSVQRGVKLIPGVKNIIA
                     VASGKGGVGKSATAVNLALALAAEGANVGILDADIYGPSQPQMLGLTGHPESPDGKTI
                     EPMMAHGIQAMSIGLLIDAETPMVWRGPMVTQALQQLLNDTRWHDLDYLVIDLPPGTG
                     DIQLTLA"
     gene            796..>2374
                     /gene="metG"
                     /locus_tag="PKZ49_11940"
     CDS             796..>2374
                     /gene="metG"
                     /locus_tag="PKZ49_11940"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HND37317.1"
                     /translation="MSSRKLLVTSALPYANGSIHLGHLVEYIQTDIWVRFQKMQGHTV
                     YYVCADDTHGTPIMLRAEKEGLTPEALIARVHGEHFSDFTGFNIRFDQYYSTHSAETR
                     HYSEDIYQKLKAAGLITVRAIEQLYDPVKQMFLPDRFVKGECPKCGAKDQYGDSCEVC
                     GAAYAPTELKNPYSAVSGATPIKKASEHFFFRLSDERCADFLRRWTREANHLQAEAAN
                     KMHEWLGQAGENKLSDWDISRDAPYFGFEIPGETAKYFYVWLDAPIGYMGSFKHFCTH
                     HGIDFDEYWKKESDTELYHFIGKDILYFHALFWPAMLENSGYRTPTQIFAHGFLTVNG
                     EKMSKSRGTFITAASYLQQQLNPEWLRYYYAAKLNGTLEDIDLNLEDFVARVNSDVVG
                     KFVNIASRCAGFITKRFEGRLVAGEDYRLLQQTIDTQFAAWQAGVIEQAYEARDFSTA
                     VRHIMKRADEVNELIHELAPWDIAKDATKTSELHRACSLGIQMFYLLSSYLKPILPAT
                     VEKIEQFLNCDSLKWPTQ"
BASE COUNT          638 a          497 c          556 g          683 t
ORIGIN      
        1 gctagggtga gttgaatatc tccagttccg ggcggtaaat cgatgaccag gtaatctaaa
       61 tcatgccagc gcgtatcatt gagtaattgc tgcaaagctt gagtcaccat tggtccgcgc
      121 cagaccatcg gggtttcagc atcgattaac agaccgatcg acatggcttg aataccatgt
      181 gccatcattg gttcaatggt ttttccatct ggagattcag ggtggccggt aaggcccagc
      241 atctggggtt gggatggacc ataaatatcc gcatcgagaa taccgacgtt agcaccttcg
      301 gctgcgagtg caagtgcaag attaacggcg gttgcggatt ttcccacccc gcctttgcca
      361 gaagcaaccg caatgatatt cttaacccct ggaatcagct taacgccgcg ttgcacgctg
      421 tgtggaataa ttttgctaaa tacgtttacc gtaaccgtac tgacgcctgc aatcgatttc
      481 aacgcttcgc tgatttgctg acgaatcgat tccagcatgg ttttggcagg ataccccagt
      541 gcaatatcga gtgaaacgtg gttatcaacg atcttaatgt tctctaccgc cttactgctg
      601 acataatctt taccagtagt ctgatcaatg actgttttca gggtttcctc aatgtgttgt
      661 tcggttagtg ccacagggtg ctccttttgc ataaatcatt aaaatgaaag atttcgatgt
      721 taacaaatat cgagggtaac tatccagttt gttacaattt acgtttataa acaatataac
      781 aaagatagtc tatcaatgag ttcacgcaaa cttctcgtta catcagcact accctatgca
      841 aatggcagta tccacctagg tcatcttgtg gaatatatcc aaaccgatat ttgggtgcgt
      901 tttcagaaaa tgcaggggca tacggtttat tatgtttgtg cagatgatac gcatggcacg
      961 ccaattatgt tgcgtgctga gaaagaagga ttgacacccg aagcgttgat cgcgcgtgtt
     1021 catggggaac attttagtga ttttacgggt tttaatattc gcttcgatca gtattacagt
     1081 acacattctg cggaaacacg ccattattca gaggatattt atcaaaaatt aaaagcggcg
     1141 ggattaatca ccgtacgtgc catcgagcag ttgtatgatc cggttaagca gatgttttta
     1201 ccagatcgct ttgtgaaagg ggaatgcccc aaatgtggtg ccaaggatca atatggcgat
     1261 tcctgcgaag tatgtggtgc cgcctatgca ccgactgaat taaaaaatcc gtattcagcg
     1321 gtttctggcg caacaccgat taaaaaagct tctgagcatt tcttttttcg gttgtccgat
     1381 gaacgttgtg ccgatttttt acggcgctgg acgcgggaag cgaatcattt acaagccgaa
     1441 gcagctaaca agatgcatga atggctgggg caagccggtg aaaataagct ttccgattgg
     1501 gatatttctc gtgatgctcc ctattttggt tttgaaatcc ccggtgagac agccaaatat
     1561 ttttatgttt ggttagatgc gcccatcggc tatatgggta gttttaagca cttctgcact
     1621 catcatggga ttgatttcga tgagtattgg aaaaaagaga gtgataccga gctctaccac
     1681 ttcattggaa aggacattct ttatttccat gcactgtttt ggcctgcaat gcttgagaat
     1741 tccgggtacc gcacccccac gcaaattttt gcacatggtt ttctaacagt gaatggcgaa
     1801 aaaatgagca aatcgcgcgg aacctttatt accgcagcca gttatttgca gcaacaatta
     1861 aatccggaat ggttacgtta ttactatgct gcaaaactta atggcacatt agaggatatt
     1921 gatctcaatt tagaggattt tgttgcacgc gtgaattccg atgtggtcgg taaatttgta
     1981 aatatcgcga gtcgctgcgc tggatttatt actaaacgat ttgaaggtcg attagttgca
     2041 ggtgaagatt atcggttatt gcagcaaacg atcgatacgc agtttgcagc ctggcaagct
     2101 ggcgtcatcg agcaggctta tgaggcacgg gatttctcga ccgcagttcg ccatattatg
     2161 aaacgtgcgg atgaggtcaa tgaattgatt catgaactgg ctccgtggga tatcgctaaa
     2221 gatgcaacaa aaaccagcga gctacatcgt gcttgtagcc tcggcataca gatgttttac
     2281 ctgctttcga gttatttaaa acccattctt ccggcaactg tagaaaaaat tgaacaattc
     2341 ctgaattgtg attccttaaa atggccaact cagg
//
LOCUS       DAOQKQ010000245         2373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_33743_length_2373_cov_4.293788, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000245 DAOQKQ010000000
VERSION     DAOQKQ010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33743_length_2373_cov_4.293788"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..671
                     /locus_tag="PKZ49_11945"
     CDS             18..671
                     /locus_tag="PKZ49_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37318.1"
                     /translation="MTRPFDTIHFTDSIEYQPPSVFGHKPPKIKIPINTGGYHASLIA
                     TYTEYTTTEYQTGGYTVSDSTVYDFAAGDPIKAHGDVGVTLYTGQRAANDYKEQLDAL
                     YQGTGRQIWSYSDSGEAFERFMAAAQMFNPIKDQANYMPAGSRFFGPGSNSNDYVAYL
                     LDAAGFGQLDGGSSILPGITTPTKITGGAPAKTITKEDIEVGTLHTFSSWKSGKAGG"
     gene            935..1900
                     /locus_tag="PKZ49_11950"
     CDS             935..1900
                     /locus_tag="PKZ49_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37319.1"
                     /translation="MEHQRDWADTNTTGNFFKATDGTVLFFPSEKRELFRQILAGQTS
                     QKLQRFLVLQKPYKHIKTQILLVSKSSDCLSVGSYTIEESELDRLRLTSGWPEVLDFC
                     QKFIVDDTGKYLPLRFSSITRLNKKEFLICGGHDYVGYKISKTFQQPARLAQVFDVSS
                     KRVIKTFPLAEIHSGNRSILLPNGNVLITGSDSPGSADPTLIEIADPRSGKSVLLKSK
                     LCAEKSGSTLSLDSEGRCLIFPGQSTIDRTDLVECLDPITDRIKVVGSMKTPRWYSDW
                     VTEIPHNSTMLARNLALVSGGSACSDHADEMFQRRDAELVSLDSP"
     gene            1931..2095
                     /locus_tag="PKZ49_11955"
     CDS             1931..2095
                     /locus_tag="PKZ49_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37320.1"
                     /translation="MSVHRLCWRVATRFGSACGEYVEDMFQRRDAEFVQLDSPQLVRY
                     FPRRHLHWSV"
BASE COUNT          604 a          552 c          519 g          698 t
ORIGIN      
        1 ctttctagtg gttgtcattg acccgacctt ttgacacaat tcattttaca gactctatcg
       61 aataccaacc tccctctgta tttggacata aacctcctaa gataaagatt ccaattaaca
      121 caggcggcta tcacgctagc ttgatcgcca cctatacgga atacacaacc actgagtatc
      181 agactggtgg ttatacagtt agtgactcaa ctgtatatga ctttgcggct ggcgatccca
      241 taaaagctca cggcgatgtt ggtgtgactc tttatacagg ccagcgggct gcaaacgatt
      301 acaaagaaca gcttgacgct ctctatcaag gaactgggcg tcaaatttgg agctacagcg
      361 attccggtga ggcattcgaa cgttttatgg cagctgcgca aatgttcaat cctatcaagg
      421 atcaggcaaa ctatatgcca gcaggtagtc gcttcttcgg tcctggttca aacagcaacg
      481 attatgtcgc ctacttgcta gatgctgctg gctttggtca acttgatggt ggttcctcaa
      541 tccttcctgg aatcaccacg cccactaaga tcactggtgg cgctccagcc aaaacaatca
      601 cgaaggagga tatcgaggtc ggaactcttc ataccttttc ctcttggaaa agtggtaaag
      661 caggcggtta gatatctaat gtgatctgcc acacttcact tctggagaaa cgcagatatc
      721 ttactcagga agacacagtt tcaaaattgt gtcttcctct tcttcaagtc cagccagatt
      781 gttatgatac taaggaacca ccgaatggaa aatagatatg agtcgtaaat ttctgatctc
      841 ctctgttctg cttctcaacg tgtttgcttg cacggtgcct ctctcatgtc tttctttacc
      901 caacagtcgt ttattgaatc ttagggcgtt atatttggag caccaaagag actgggctga
      961 taccaacacc actggtaact tctttaaggc tactgatgga acggtactat ttttcccatc
     1021 tgaaaagagg gaattgtttc gacaaatact tgcaggccaa acttcgcaaa aactgcaacg
     1081 gtttttagta ctccaaaaac catataaaca catcaagact cagatcctcc tcgtttctaa
     1141 gtcttcagat tgtttgtcag ttggatctta tacaattgag gagtcggaac ttgatagact
     1201 taggcttact tccggctggc ccgaagtatt ggacttctgt caaaaattta ttgtcgatga
     1261 tactggcaag tatcttccgc tccgcttctc ttccataacg cgactaaata aaaaggaatt
     1321 tctaatatgt ggagggcacg actatgtggg ttacaagata tccaagacgt ttcagcaacc
     1381 tgcgagactc gcccaggtat ttgacgtttc aagcaagcgt gtcattaaaa cctttccgct
     1441 cgcagaaatc cattcaggca atcggtcgat tttgttgcca aatgggaatg tattaatcac
     1501 aggcagtgac agtccgggca gtgctgatcc tacgttgatt gaaattgctg atcctcgttc
     1561 tggtaaaagt gtattgctca aatcaaagct atgcgctgaa aaatctggct caactctttc
     1621 tttggattct gaggggcgat gcttaatttt tcctggtcag tcaacaatcg atcgcaccga
     1681 cttagtagag tgtcttgacc ctattacgga tcggattaag gtagttggga gtatgaaaac
     1741 cccccgttgg tattctgatt gggttactga aataccccac aactctacta tgctggcaag
     1801 aaatttggct cttgtttctg gaggatcggc atgcagtgat cacgcagatg agatgtttca
     1861 acgccgtgac gctgagcttg tgtcacttga ctcaccgtag ctcgaaagtg acaaacacct
     1921 gtttaatctt gtgtctgtgc atagattatg ctggagagtt gctactcgtt tcggttcagc
     1981 ttgtggcgaa tatgttgagg atatgtttca gcggcgtgat gctgagtttg tacagcttga
     2041 ttcccctcaa ctggttaggt atttcccccg tcgccatttg cattggtcag tctagtaacg
     2101 atcgtttata gcgaaaagtc gtaggctgaa acgcctacca actcaccctt ttggggagcc
     2161 gtaattatca ccgcccctgg ctgtgctctt tacggctccc tctttttttt gatctttaac
     2221 accttctact aaactatgtc acaagcgtct tggctcccaa tgctgggctg gctttcatgc
     2281 ttcctcggct atgttgaaat ggtcaccaaa aactcgccgg cagcttcaat cgtcaagcag
     2341 tctccagagt ttggagttaa gtcttggacg gtt
//
LOCUS       DAOQKQ010000246         2300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_35690_length_2300_cov_6.240089, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000246 DAOQKQ010000000
VERSION     DAOQKQ010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35690_length_2300_cov_6.240089"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            568..1467
                     /locus_tag="PKZ49_11960"
     CDS             568..1467
                     /locus_tag="PKZ49_11960"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0019580 - galactarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HND37321.1"
                     /translation="MNIGFIGLGIMGKPMASHLIKGGHTLYLHSRSGVPEELIILGGR
                     ACSSPGQVAKNSTVIITMLPDTPDVEAVLFGTDGVTQGLTADPTSPTKTIIDMSSISP
                     IETKKFAEKLSKYGCNYADAPVSGGDIGAQNATLTIMVGASESVFNEIQPLLALMGST
                     ITLVGDVGSGQICKIANQIIVALTIEAVAEALLFTSKAGADPQKVRQALMGGFAASRV
                     LEVHGNKMINRTFEPGFKIALQQKDLNIALSCASELGMSLPNTAVTQALYNACGAQGG
                     NKWDNSALVRILEIMANHEIQQH"
     gene            1470..>2300
                     /locus_tag="PKZ49_11965"
     CDS             1470..>2300
                     /locus_tag="PKZ49_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HND37322.1"
                     /translation="MSKQDLINQFRTFLSADSVLFESEDLKPYECDGLSAYRQVPLIV
                     VLPETEEQIISILQICHTTQTPVVARGAGTGLSGGALPIDNGVLLSLAKLKRILEVDP
                     VARTARVQPGVRNLSISQAAEPFGLYYAPDPSSQIACTIGGNVAENSGGVHCLKYGLT
                     VHNILKLRVLTIEGDILEIGSEALDSPGYNLLALMTGSEGMLGIVTEVIVKLIPRPEK
                     AQLVMAAFDDVQKAGDAVAQVISAGIIPAGMEMMDRITIHAVEDFLHAGYDLDAEAIL
                     L"
BASE COUNT          688 a          461 c          497 g          654 t
ORIGIN      
        1 cagagccgtc agatttacag tctgatccct ttgaccgctc gggaacccct ccgaaataat
       61 ctcgcattat ggatgcattg ttattgcaag tcaataaata gctgacagaa caaataagct
      121 tccccagttt ctagcacaaa atgacttgtt gggaagttta atatccgtaa ggagaattga
      181 aaaactgcta gcacagatat ctgtcaattc aattcgtaaa accattagta aattatcctt
      241 agtaaattga ttatatctaa taatcgattg aagctgggtc tgatctatgt gcgtcttagc
      301 gtaattcgat caacacggat tatcatccat aaagctgttt gtgattgcac acacttgagt
      361 ttgcctttac agctcaaccc ctcccgcttt tccgttacag cttttacaac catctcattg
      421 ttgagtcgat tcaattttaa tacgattgcg ctaagcaacg agtcttatta ttgtggcaaa
      481 atcactatcg ttaaaaaata attagcaaat ttttcagtgg taatgctacg agaaaaatca
      541 agttagtttt ctgcaaagga attgtaagtg aatatcggat tcattggttt aggtatcatg
      601 ggaaaaccca tggcgagtca tttaattaaa gggggacaca cgctttattt acattctcgc
      661 agtggtgttc cggaagaact catcatatta gggggacgag cctgctcctc accgggtcaa
      721 gttgccaaaa attcgacggt aatcattacc atgttaccgg atacaccgga tgttgaagca
      781 gtattatttg gtaccgacgg tgtcactcag ggattaactg ctgatccaac ctcaccgact
      841 aaaacaataa tagatatgag ttctatttca cccatcgaga ccaaaaaatt tgctgagaaa
      901 ctcagtaaat atggctgtaa ttatgcagat gcgccagttt cgggagggga tatcggcgca
      961 caaaacgcta cgcttaccat catggtaggt gcttcagaat ctgtgtttaa tgaaatacaa
     1021 ccactattag cgctaatggg tagcacaatt accttagttg gagatgtagg ttcaggacag
     1081 atctgtaaaa tagcaaacca aatcatcgtt gcgttgacta tcgaagcagt agcagaagca
     1141 ttattattta catcgaaagc tggtgctgac ccacagaaag tcaggcaagc attaatgggc
     1201 ggatttgcag catctcgtgt gcttgaagtg catggcaata aaatgattaa tcggacattt
     1261 gaaccaggct tcaaaatagc gttacaacaa aaagatctga acatcgcgct atcatgcgct
     1321 tcagaacttg gaatgagttt accaaatacg gcagttactc aggctttgta taacgcatgc
     1381 ggtgctcaag gaggaaataa atgggataat tcagcacttg ttcgtattct tgagataatg
     1441 gctaatcatg agattcaaca gcattaacga tgtctaaaca agacctaatt aaccaatttc
     1501 gtaccttcct ttctgcagat tcagtattgt ttgaatctga agatttaaag ccatacgagt
     1561 gtgatggttt atctgcctat cggcaagtgc ccctgatcgt tgtattaccg gaaactgaag
     1621 aacaaattat cagcattcta caaatttgcc atacgacaca gacgcccgtt gttgcgcgcg
     1681 gtgcaggaac tggcctatcc ggaggtgcat taccgatcga taatggtgtt ttactatccc
     1741 tagcaaagct taaacgcatc cttgaagttg atccagttgc acgaactgca cgagttcaac
     1801 cgggtgtgcg taatctctcg attagtcaag cagccgaacc atttgggctg tattatgcgc
     1861 ctgatccttc atctcaaatt gcttgcacga ttggcggtaa tgttgcagaa aattcgggcg
     1921 gagtacattg cctaaaatat ggattgactg tccataatat ccttaaattg agagtgctaa
     1981 cgattgaagg tgatatcctt gaaatcggta gtgaagcttt ggatagccct ggttataacc
     2041 tactcgcttt gatgacaggt agcgaaggta tgctcggcat tgtcactgaa gtaatcgtta
     2101 aattaattcc taggcccgag aaagctcagc tagtcatggc tgcgttcgac gatgtgcaaa
     2161 aagcaggtga tgcagtagca caagtcatta gtgctgggat tattcctgcc ggaatggaaa
     2221 tgatggatag aattactatc cacgcagtag aagatttttt gcatgcaggc tatgaccttg
     2281 atgctgaagc aatattactc
//
LOCUS       DAOQKQ010000247         2220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_38117_length_2220_cov_6.097921, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000247 DAOQKQ010000000
VERSION     DAOQKQ010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38117_length_2220_cov_6.097921"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..1293)
                     /gene="hisS"
                     /locus_tag="PKZ49_11970"
     CDS             complement(31..1293)
                     /gene="hisS"
                     /locus_tag="PKZ49_11970"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HND37323.1"
                     /translation="MSSTIRAIRGMNDILPDEVYQWDFFETTIRDWLAAYGYRNIRTP
                     IVEQTDLFVRSIGTATDIVEKEMYTFVDHLNGESLTLRPEGTASCVRAVLEHSLLYTG
                     PQRLYYYGPMFRHERPQKGRYRQFHQVGVEAIGYANAEIDAELIIMCARLWRQLGISN
                     LRLEIGTLGSVASRAVYRDRLIGYFEQHLQHLDEDARRRLYTNPLRILDSKNPAMHEL
                     ILNAPRLLDDLDEESLVHFKELQDRLQQQGIDFEINLKLVRGLDYYNRTVFEWITDKL
                     GAQGTVCAGGRYDGLIEQMGGKHTPACGFAMGIERILALMNETGVKIPQSEVDAYVVY
                     QGEMAAKFCWKVAEYFRDNGLKVILHCGGGNFKSQMKKADASGARFAIIIGDDEANDS
                     EVSIKPLREAVEQVRVGLNEAVALLKQH"
     gene            complement(1286..>2220)
                     /gene="ispG"
                     /locus_tag="PKZ49_11975"
     CDS             complement(1286..>2220)
                     /gene="ispG"
                     /locus_tag="PKZ49_11975"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HND37324.1"
                     /translation="PACAKALAKYRINPGNVGHGKKRDEQFATLIETACHYDKPVRIG
                     VNWGSLDPEMLARIMDENAKSSDPLDAGQVMRKALITSALESAAKAEELGLPRDHIIL
                     SCKVSGVQDLIAVYRDLAAQCDYALHLGLTEAGMGSKGIVASTAALSVLLFEGIGDTI
                     RISLTPEPGGDRSREVIVAQEILQTMGLRAFVPLVAACPGCGRTTSTYFQELAKSIQT
                     YVREQMITWRDEYDGVENMTLAVMGCVVNGPGESKHANIGISLPGSGENPVAPVYVDG
                     QKTVTLKGDNIAGEFRQIIDDYVRTKYQRKVANV"
BASE COUNT          609 a          587 c          405 g          619 t
ORIGIN      
        1 gccacgattt cttcctagta ttttaaaaaa ttaatgttgt ttaagtaacg caaccgcttc
       61 atttaaacca actcgtactt gttcaactgc ttctcgcaac ggtttaatac ttacctcgct
      121 atcgtttgct tcatcgtcac cgataatgat cgcgaaacgg gctccactgg catccgcttt
      181 tttcatctgt gatttaaaat ttccgccccc acaatgcaaa ataaccttga gcccattatc
      241 tcgaaaatat tcagcgactt tccaacaaaa ttttgcagcc atttcacctt gataaactac
      301 ataagcatct acttcagatt gaggaatttt tacgccggtt tcattcatca acgccaggat
      361 ccgttcaatt cccattgcaa aaccacaggc tggggtatgc ttccccccca tctgttcaat
      421 caatccatcg tatcgcccac cggcacaaac cgttccttgt gcacctaatt tgtcggttat
      481 ccactcaaat accgtacgat tgtagtaatc taaccctctc accaatttga ggttgatctc
      541 aaaatcaata ccctgctgct gtaatcgatc ctgcagctct ttaaaatgaa ccagtgattc
      601 ctcatctaaa tcatcaagta gcctaggcgc attaagaata agttcatgca tggctggatt
      661 cttactatct agaattcgca acggattggt atataaccgt cgtcgggcat cttcatctaa
      721 atgctgtaaa tgctgttcga aataaccgat aagccgatca cgataaaccg cacgggaggc
      781 tacgctgcct aaagtaccaa tctcaagtcg taaattggaa atacctaatt gacgccataa
      841 tcgagcacac ataatgataa gctccgcatc aatctctgca ttagcgtaac cgattgcttc
      901 aacacccact tgatgaaatt ggcgataacg ccctttctgg ggacgctcat gccgaaacat
      961 cggcccatag taatataatc gttgtggtcc tgtgtaaagc aaactatgtt caagcactgc
     1021 gcggacacaa gatgccgtac cttcagggcg taatgtcagg ctctctccat ttaaatgatc
     1081 aacgaaagtg tacatttctt tctcaacgat atcggtcgcc gttccaatag agcgcacaaa
     1141 caaatcggtt tgctcgacga ttggcgtccg aatattgcga taaccataag cagccaacca
     1201 gtctcgaatc gttgtttcaa aaaaatccca ctgatatacc tcatcgggta ggatgtcatt
     1261 catcccccga atggcacgga tcgtactaga cattcgctac ttttctttga tatttagtac
     1321 gtacataatc gtcgataatt tgacgaaact cccctgcgat attatcacct tttagtgtca
     1381 ctgttttctg gccatcgaca tagaccggcg ctaccggatt ttcccctgag cccggaaggc
     1441 ttattccaat attggcatgc ttactttcac cgggaccatt caccacacaa cccatcacag
     1501 cgagtgtcat attctcaaca ccatcatact catctcgcca tgtaatcatc tgttctcgga
     1561 cgtaagtttg gatactcttt gccaattctt gaaaataagt actggtcgtt cttccacatc
     1621 ccggacaagc ggcaactagt ggaacaaatg cgcgcaatcc cattgtttgc aagatctctt
     1681 gcgccacaat cacttcacgc gagcggtccc ctcccggttc tggcgtcaaa gaaattctga
     1741 tcgtgtcacc aataccttca aacaataata cagataatgc ggctgtagac gcgactatgc
     1801 cttttgaacc cattcccgct tctgttaatc ctaaatgcaa tgcataatca cattgagcag
     1861 ccaaatctcg gtaaactgca attaaatcct gcacgccact tactttgcaa gacagtatga
     1921 tgtgatcacg tggcaaaccg agttcctcag ctttagctgc actttccagc gcagaagtaa
     1981 tcagtgcctt acgcatgact tgacctgcat ccaatggatc actagatttt gcgttctcat
     2041 ccatgatacg agcgagcatt tcagggtcca gactacccca attaacaccg attcggacag
     2101 gtttgtcata atggcaagca gtctcaatca gggtagcaaa ttgctcgtca cgttttttac
     2161 cgtggcctac gttaccggga ttaatgcggt actttgctaa ggccttagcg caagcaggat
//
LOCUS       DAOQKQ010000248         2153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_40240_length_2153_cov_3.361296, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000248 DAOQKQ010000000
VERSION     DAOQKQ010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40240_length_2153_cov_3.361296"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="PKZ49_11980"
     CDS             complement(<1..595)
                     /locus_tag="PKZ49_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HND37325.1"
                     /translation="MIRYISQKQLPLEGFDTPPGMILDPTNRWVKLRDCLPWDELSES
                     YYKTLCSNLGRPAKDAGIVIGAVIIKHKLSVSDEETVEQIRENPYLQYFIGLKGFQAQ
                     AAFASSLLVEIRKRMGQTVFDEFHEAIIETVEPRRTKKSVKASDDDNDGNDVSNSGDT
                     GSNDAGTAMEADQSLADELPRNHGKLILDATVAEQAIR"
     gene            complement(779..1279)
                     /locus_tag="PKZ49_11985"
     CDS             complement(779..1279)
                     /locus_tag="PKZ49_11985"
                     /inference="COORDINATES: protein motif:HMM:NF013676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HND37326.1"
                     /translation="MHQADRTYLSPSRQRLNSYSKQHKLYQALKSFGQIIKSEFILRY
                     IDDVELRQAIERQLNKVELANRFTRAIAVGNPRELTQGDKEEQEIAESCNRLIKNSII
                     CWNYLYLSQKLERIDNPEQKDKLLSAISSHSPMSWSHTNLLGEYDFSDEKLKDTAGIR
                     LPRIAT"
     gene            complement(1335..1688)
                     /locus_tag="PKZ49_11990"
                     /pseudo
     CDS             complement(1335..1688)
                     /locus_tag="PKZ49_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1795..2079)
                     /locus_tag="PKZ49_11995"
                     /pseudo
     CDS             complement(1795..2079)
                     /locus_tag="PKZ49_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611352.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          530 a          488 c          474 g          661 t
ORIGIN      
        1 agcgtatggc ctgttctgca acggtcgcat ccagtattaa tttgccgtga ttgcgcggca
       61 gctcatcggc caaagattgg tctgcctcca ttgcagtacc cgcgtcattg ctgcccgtat
      121 caccgctatt tgacacatca ttgccgtcgt tgtcatcatc acttgctttg actgacttct
      181 ttgtccgtcg aggttcaacc gtttcgatga tcgcctcatg aaactcatca aatactgtct
      241 gacccatgcg cttgcgtatc tcaaccagta atgaggacgc aaaggccgcc tgcgcttgaa
      301 aacctttcag accaataaaa tattgcaggt acggattttc tcgaatctgt tcaacagtct
      361 cttcatctga gaccgataat ttatgtttaa taatcaccgc tccaatcaca attccggcat
      421 ctttggctgg gcggcccaga tttgaacata acgtcttata gtagctctcg gacaactcat
      481 cccacggtag gcaatccctc agttttaccc agcgattagt gggatctaga atcatgccgg
      541 gcggtgtatc aaatccttcg aggggcagtt gcttttggct gatgtaacga atcatagtgc
      601 acggtttttg gtgttaattc acatatttat gtgcactatt ctaccaaaaa acaaaatatt
      661 aataaattca atcagctagt tatacttttt gagtaagctc taattaccaa taggattttt
      721 atagaagcat tctgggcgtt tatcacaatg attttgaggc tgctattatt ggtgttgatt
      781 atgttgcaat tcttgggagt cggattccag cagtatcttt aagcttctca tctgaaaaat
      841 catattcacc aaggagattg gtatgcgacc atgacatagg ggaatgcgac gaaatcgcag
      901 acaggagctt atctttttgt tcaggattat ctatccgttc cagtttttgt gataagtaca
      961 gataattcca gcagataatg gaatttttaa tcagtcggtt gcagctctcg gcaatttctt
     1021 gctcttcttt gtctccttgc gttaattctc gtggatttcc gacggcgatt gctcgtgtga
     1081 acctgttggc tagttcgact ttgttgagct gcctttcgat agcctgtcgt aattcaacat
     1141 catcaatata acgaaggata aattcggatt ttatgatctg cccaaaggat ttaagggctt
     1201 gatataattt gtgctgtttc gaataggaat tgagtcgctg acgactaggt gacaaatagg
     1261 tacgatctgc ctgatgcact gaccccggtc ataacttcca gggtgacatg gtcaagtaaa
     1321 accggacaat tcagttaagc aactttcttt acattttcaa tttctacctc atattgatta
     1381 gggctcttgt accccaggta tgagtgcagt cgatgagtgt tataaaacat ggaaatgtac
     1441 tgcaacacat cttgctgagc tgcgaaacgg gtttgataat ggcgccggtg gattctctcc
     1501 tgttcgaggc tgccgaaaaa gctttctgcc acaacattgt cgaaacaatc gccggcacgg
     1561 ctcatactac ccacaaagcc ttgtatttcc agaattttgt ggcactccct gctggcatat
     1621 tgtgatccgc gatccgaatg cactatcagt cctgcttgcg gctggcgctg ccagatcgcc
     1681 attctcagtg tagagttgtc ccattttgac ggacagtttt ttcatggagt cgagaaagtc
     1741 tcgacctgtg aaagcagact gcagtttatc tgcgatttga ttattctcat tcagttttct
     1801 ttcaaaattg atcggtgata ggtatcccag tccagaatgc ctgcgagtgg ggttgtacca
     1861 ggactcgatc caagtgaata tggcgagcct tgcttcagtt ttgtttttcc aggaacgcct
     1921 gtcgatcagc tcacattcca gggaagcaaa aaagctctca gccattgcgt tatcataggc
     1981 atcacccaca ctgcccatct aaggatgcac gcccatttcc ttgcatcgct tgccgaactc
     2041 aacgctggta tattgattgc cttgatcgct gtggtgaatt gtttagtccc gcagtatgct
     2101 ggtgtaatat acagccatct acggagggag gatttatggg acagattttg cac
//
LOCUS       DAOQKQ010000249         2116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_41544_length_2116_cov_6.176613, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000249 DAOQKQ010000000
VERSION     DAOQKQ010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41544_length_2116_cov_6.176613"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1684
                     /locus_tag="PKZ49_12000"
     CDS             <1..1684
                     /locus_tag="PKZ49_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HND37327.1"
                     /translation="DYSWADMARWVVRWQAALTREDLSPGDRVAIMCRNCPQWVAFEQ
                     AALGLGLVVVPLYTEDRAENAAYCIENAGVKLLVVEKSTQWEAFLPIIAQLSSVLRIV
                     ILLVTDDATKRGYDPRVVTLQQWLPEQGEQMLHINTNPDALATIIYTSGTTGRPKGVM
                     LSHDNILSNTFASTQAVDVLPDDLFLSFLPLSHTFERTSGYYVPMMCGATVAYARSIQ
                     LLQEDLLTIQPTVLVSVPRIYERVYAGLRTKLAEGSVLARKLFYFAVSVGYSRFEQQQ
                     GRDTWHFSHLFWPILEKLVARKLMDKLGGRLRLAMSGGAALSPEISRIFIGLGLPILQ
                     GYGMTESSPVVCVNRLDDNLPSSIGRTISGVEVKLGENNALLIRGPNVMLGYWNNPEA
                     TKAVISEDGWLNSGDIAQIDEQGRVTITGRLKDIIVTSTGEKVPPADMEAAILRDPIF
                     EQVMVVGEGRSYLGALVVLSQRGLKTLAELCHLPPDPHQLINQESVEKILLDRIAQQI
                     HEFPGYAKIFKVALIPEPWSVANEMLTPTLKLKRSKIAQRYQAEIENLYTKR"
     gene            1714..>2116
                     /locus_tag="PKZ49_12005"
     CDS             1714..>2116
                     /locus_tag="PKZ49_12005"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HND37328.1"
                     /translation="MPTNSVTSDLSLHVPLPESQPVLRMVPMPSNTNYAGDIFGGWIM
                     SQVDIAGSIPALRRARGRVATIAVNSFVFKQPVLVGDLVSFYADIIKIGRTSITVDVA
                     VYAQRGVREGGEEICIKVTEAVLTYVAIDTDR"
BASE COUNT          551 a          491 c          521 g          553 t
ORIGIN      
        1 cgactacagt tgggctgaca tggcacgatg ggttgtacgc tggcaagctg cgctgacgag
       61 ggaagatcta tcccctggtg atcgggttgc cattatgtgc aggaactgcc cgcaatgggt
      121 agctttcgag caagcagcac tggggctcgg attggtcgtc gtccctttgt ataccgaaga
      181 tcgtgcagaa aatgcagcct attgcattga gaatgcggga gtaaaactgc tcgttgtcga
      241 aaaatcaacg caatgggaag cttttcttcc aattattgct caactcagct cggtattacg
      301 gattgtaatt ctattagtca cggatgacgc aacgaaacga gggtacgacc cgcgcgtcgt
      361 tacgctgcaa caatggttac ccgagcaagg ggaacaaatg ctgcatatca ataccaatcc
      421 cgatgcgctg gcaacgatca tctatacctc tggaaccact gggcgaccca agggggttat
      481 gctaagccac gacaatatcc tgtctaatac ctttgcttcg acacaggcgg tagatgtctt
      541 acccgatgat ctatttcttt cgtttctacc tctctctcat acgtttgaac gaacctctgg
      601 ctattatgta ccgatgatgt gcggggcaac ggttgcctac gctcgctcca ttcaattatt
      661 gcaagaagat ttactgacga ttcagccaac cgtgctcgtt tcggtgccac ggatttacga
      721 acgggtttac gccggtttgc ggaccaaact cgcagagggt tctgtgctag cacgcaagct
      781 attctatttt gctgtatcag taggttacag ccgttttgaa cagcagcaag gtcgtgatac
      841 ttggcatttc tcccatctat tctggccgat cctcgaaaaa ttagtcgcgc gcaaactcat
      901 ggataaatta ggtggtcgat tacgccttgc catgagtggt ggtgctgcat tatcgcctga
      961 gatatcacgt attttcattg gcttggggct cccaattctg caaggatatg gcatgacgga
     1021 aagcagtcct gtagtttgtg tcaaccgact cgatgacaat ctaccttcca gcattggccg
     1081 aacaatttca ggcgttgaag ttaaactggg agaaaacaat gcattattga ttcgcggccc
     1141 gaatgtcatg cttgggtatt ggaataatcc agaagcgacg aaagcagtaa tctcggaaga
     1201 tgggtggctt aactcaggag atatagcgca aatcgacgaa caaggaagag tgacgatcac
     1261 cggtcgatta aaagatatta tcgtaacctc aacgggagag aaagttccgc ctgccgatat
     1321 ggaagcggcc atcttgcgtg atcccatctt cgaacaagtc atggtggtag gcgaaggccg
     1381 ctcttatctc ggcgcgttag ttgtattaag tcagcgagga ttgaaaaccc ttgccgagtt
     1441 gtgtcactta ccgcctgatc ctcaccaatt gattaaccag gagtcagtag aaaaaatact
     1501 actcgatcgc attgcgcagc aaatccatga atttccaggc tatgccaaaa ttttcaaagt
     1561 tgctttgatt ccagagcctt ggagcgtcgc caatgaaatg ctaacaccca cgttaaaatt
     1621 aaagcgttct aaaatcgcac aacgctatca agctgaaatc gagaatctct acactaagcg
     1681 ttgaatcata ggtttaacga cacctatcac accatgccaa ccaactcagt aacaagcgat
     1741 ctatctttgc atgttccact tccggaaagc caaccggttt tgcggatggt gccaatgcct
     1801 tccaacacca attatgcagg ggatatcttt gggggttgga tcatgtccca agttgatata
     1861 gcgggtagca ttcctgcatt gagacgggct agaggtcggg ttgcaaccat tgcggttaac
     1921 tcttttgttt tcaagcaacc ggtattagtc ggtgatttag tcagttttta tgcggatatt
     1981 atcaaaattg gacgaacctc gattaccgtt gatgtcgcgg tctacgcaca acgcggggta
     2041 cgtgagggtg gtgaagagat ttgtatcaaa gtaaccgaag ccgtgctgac ttatgttgcc
     2101 attgataccg atcgac
//
LOCUS       DAOQKQ010000250         2041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_44352_length_2041_cov_4.585096, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000250 DAOQKQ010000000
VERSION     DAOQKQ010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44352_length_2041_cov_4.585096"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PKZ49_12010"
     CDS             <1..261
                     /locus_tag="PKZ49_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37329.1"
                     /translation="SLPPPLKSFYLRKTRGDSIAESIDISAYALKRLVNHKMSNGVTA
                     GYIVSDVERLRKPMQQISSQLLRYFRIDDEKRVLSFPVYQST"
     gene            286..552
                     /locus_tag="PKZ49_12015"
     CDS             286..552
                     /locus_tag="PKZ49_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HND37330.1"
                     /translation="MCKSYNLLIRSQAKKKLQSLPRADRSRIAEKLEQLGRDPDNSSL
                     DIKKLEGGPYFRLRVGNWRVIFERHDVVRIITIEKIKPRGDAYK"
     gene            549..881
                     /locus_tag="PKZ49_12020"
     CDS             549..881
                     /locus_tag="PKZ49_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HND37331.1"
                     /translation="MTLQKIKSIHGKDEYVLLPIAVYRVLKDQIEKELATCEANRDDE
                     QSYEPFVLEDYVDNPVALARIKAGITQEQLAQRLGVSQAYVSQIERRNNVTNKVLERV
                     YTAIQGTT"
     gene            1024..1206
                     /locus_tag="PKZ49_12025"
     CDS             1024..1206
                     /locus_tag="PKZ49_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37332.1"
                     /translation="MFLDSDDFKSVWELAHNWISEDTTKTESESISADLKVAIHQLLH
                     AIVSISIENCPPVLVG"
BASE COUNT          695 a          399 c          384 g          563 t
ORIGIN      
        1 tctcttcctc ctcctctcaa atcattttat ctccggaaaa cccggggcga ttcaattgct
       61 gaaagcatcg acatatccgc ttacgcactc aaacgattgg ttaatcataa gatgtcgaat
      121 ggtgtgacgg ctggttatat cgtcagcgat gttgaacgat tgcgtaaacc catgcagcaa
      181 atatcctctc aattacttcg atactttaga atcgatgatg aaaaaagagt actgtcattt
      241 ccagtgtatc aaagcacttg aatataatat tttaaaactt ataatgtgtg taaatcttat
      301 aatctactaa tacgatccca agcaaaaaag aaattacaaa gcttgccaag agcagaccga
      361 tctaggattg ctgagaaatt agaacagcta ggtcgggatc cagataatag ttcacttgat
      421 atcaaaaaac ttgaaggtgg gccttatttc cgtctgagag ttggtaattg gcgtgttatt
      481 tttgagcgac atgatgtcgt tagaattatt acgattgaga aaattaaacc gcgtggagat
      541 gcttacaaat gacactgcaa aaaatcaaat ccatacacgg taaagacgaa tatgttctat
      601 taccaatcgc agtttatcgc gttctaaaag atcaaatcga aaaagaatta gcaacctgcg
      661 aagcaaatcg agatgacgag caatcttatg agccatttgt tctcgaagat tatgttgata
      721 atcctgtagc gttagcacga atcaaagccg gtattacaca agaacaatta gctcaacgct
      781 taggtgttag ccaagcatat gttagccaaa ttgagcgtcg caataatgta accaacaaag
      841 tacttgagcg agtgtacaca gccatccaag gaaccacata aatttaggaa atcactgctt
      901 aattcatcat tgccgccgcg aagcggcttg tccttgacag cttgtcactc cccacacaat
      961 gagaattgaa ggattgagga ataagcacaa ccaccccttc catttctaac aaaagaatat
     1021 tcaatgtttc ttgatagtga tgactttaaa tcagtttggg aattagctca taactggatt
     1081 agtgaggata caaccaaaac tgaatctgaa tcaatttctg ctgacctcaa agtggcaatc
     1141 catcagctgt tacatgctat tgtgtcaatc agcattgaaa actgtccacc tgttttagtt
     1201 ggttaattgg tttttacatt attttcgatg cttgaaacgg taagaatcat taccggtttc
     1261 caggatgtcg caatgttgct tttcttgact taccaccttg ctggatatct ataaacgcaa
     1321 ccagtaacca agcagtaaaa aataatgcta tcagctcacg aaccataaaa gagaaaaggc
     1381 tacagcaaca acatgctgct catcagaaac ataaacctgt tgaggagcta aaaaaagcat
     1441 gcattcttta ctggttggaa aatcaaagtt gcagcaatga gcaaactgca aaaaaatttt
     1501 atggatcact ttcttctgag caacagaaac tgctcgttcc aaccaatgca gctaaaacgc
     1561 ttgcaaaagc tatatccgac tatagaaatc gagataaatt agcaaaaaag aataacctac
     1621 cgtattggtt atgaatatta cgacttcggt tgacagttct gataaattaa actgttttca
     1681 aggagtcgtt gacgaatcta caagcacaaa gagatatatc tcgcaaactc aaagtatttg
     1741 aacatgccgc tacatctggg aatattgcat tcacctgcag gcattttggt atatcaagag
     1801 atacatttta tcaatggaag cgggcttata cggcacgtgg agaaaaaggg ctgatcaata
     1861 gcaaaccatg ccctgaaaac cccaagagac ttttgcaaaa cacctgactt gataaaatcg
     1921 ggcaaagata gtgcgattcc agtctgattt actcaatttt ttatgaattt ttataaaaaa
     1981 tacgtgaatt agtcggtcct gcaaaattaa cttaaccgtg gcggaggttt tgtatacaag
     2041 a
//
LOCUS       DAOQKQ010000251         1955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_48094_length_1955_cov_3.921579, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000251 DAOQKQ010000000
VERSION     DAOQKQ010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48094_length_1955_cov_3.921579"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(485..1378)
                     /locus_tag="PKZ49_12030"
     CDS             complement(485..1378)
                     /locus_tag="PKZ49_12030"
                     /inference="COORDINATES: protein motif:HMM:NF038126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxDxF family PEP-CTERM protein"
                     /protein_id="HND37333.1"
                     /translation="MKFELKKSHIAAAIALALSGAAFTTAHAAVTITDGAGTSARIAD
                     AGYFASGGLGLSYLGKEFVNWDTEASNYTLTVGGVKVGGASADEVFGSNPLGASVIGP
                     LFGGTTAITANFGDPNGVKWNIFETVRVSAPGHVLVNVSLTNIGTTIAKDVEWSVGID
                     PDQGRNSLPNGPGNWTHNVINATGFGSSVSATSLDGLSLTLANVTGGTAVGVASYIDT
                     ACCSPVTASTIFGAGQAAGYISDGDYSINLGYQLGDIAPGYSSSIAYEYIMAVPEPET
                     YAMLLAGLGLIGFSARRRVAV"
     gene            1737..>1955
                     /locus_tag="PKZ49_12035"
     CDS             1737..>1955
                     /locus_tag="PKZ49_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HND37334.1"
                     /translation="MINTEEQTTALTQENDAQAEPIKTVKIDSSSITLLGTAHVSKAS
                     ADKVQELIATGQYDAVAVELCPSRHRAIV"
BASE COUNT          599 a          479 c          353 g          524 t
ORIGIN      
        1 tggatcacct aaacaacagc taaaaatact caaactttgg actcagattt aacctgatct
       61 actacagccc caaaaatata gtagcaatga gtctcttcat aacaactgct tccctctcaa
      121 gtcatttcac tggcaaatac ctttttacag attcattttc atgaattgta cactgttatc
      181 tgcttgctcg agcttgcatt atgagattga tcgaagcaat ccatttttga ttgactctgt
      241 atataaaacc gaaacagtca atcaaattga aaagtaaatt tcccatccgg atagattatt
      301 gattccttat gccatcgata gagaaacaac tcaacaagtt taaacttccg ataaagctta
      361 tcgtaaccgt tcactggaat tttttacctc tactgacaat gaatattgcc cagctttaaa
      421 taatacaaaa aaagccacag agaatattgc atctctgtgg ctgctaacta aaattcgctt
      481 ctaattacac tgctacgcgg cggcgtgctg agaaaccgat caaaccaagg ccagccagca
      541 acatagcgta ggtttctggt tccggaacgg ccatgatgta ttcgtaagcg attgaactac
      601 tatagccggg tgcgatatcg ccgagttgat aacccaaatt aatcgaataa tctccatcgg
      661 aaatataacc ggcagcttgt cccgcaccaa atatagtact tgctgtaaca ggactgcagc
      721 acgcagtgtc aatgtacgaa gctacgccga ccgccgttcc cccagtaaca ttagccaacg
      781 taagcgataa accatcgagg cttgtcgcag ataccgagga accaaatcct gtcgcattaa
      841 tcacgttgtg cgtccaatta ccagggccat taggcaatga attcctgcct tgatcgggat
      901 caataccgac actccactca acgtctttag ctatagtagt gccgatattc gtcaaactca
      961 cattaactag gacatgacca ggcgcactaa cgcgtactgt ctcaaaaata ttccacttaa
     1021 ccccattggg atcaccgaaa tttgcagtga ttgctgtcgt gccaccaaac aaaggcccaa
     1081 tgactgacgc acccagcgga ttagagccga atacttcatc agcagatgca ccaccaactt
     1141 ttacaccacc gactgtcagt gtgtaattag atgcttcagt atcccagttg acaaactcct
     1201 tacccaaata gctcaagcct agaccaccgg acgcgaaata tccagcatcg gcaattctag
     1261 cggaagtacc tgcaccatct gtgatggtaa ccgcagcatg cgctgttgta aaagcagcac
     1321 ctgatagagc aagcgctatt gccgcagcaa tgtggctttt tttcaattcg aatttcataa
     1381 ttcactcctt aaatttatag ttgcaacata aaatagttgt tagaatcttc gctctttaca
     1441 actgatttaa ttttcaaacc actcattaac caattacgct ttgcatattc tcaatgcaaa
     1501 atttatttaa caattacaac atattacagc gtgctctatt atagatttgg aatcttttta
     1561 gtacatagaa cattcctccc tttttgctag tacacgtgta ctacaattgt cacgtcagaa
     1621 tattattaca tagcattgcc agaattgttt acacttaatt ttgttttaac tgatgccgcg
     1681 ataatttccg gtgttgccat ggtttccatt tgcgatgatt cttaaccgtc acatatatga
     1741 tcaatacaga agaacaaacg accgccttaa cccaggaaaa tgacgcacaa gcggagccga
     1801 tcaaaacagt caaaatcgat agcagcagta ttaccttact gggaaccgcg catgtctcaa
     1861 aagccagcgc ggataaggtt caagagctca tcgccaccgg gcagtacgat gccgttgcgg
     1921 tggagttatg cccaagccgg cacagagcca tcgtg
//
LOCUS       DAOQKQ010000252         1953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_48166_length_1953_cov_5.630664, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000252 DAOQKQ010000000
VERSION     DAOQKQ010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48166_length_1953_cov_5.630664"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..728)
                     /gene="rdgB"
                     /locus_tag="PKZ49_12040"
     CDS             complement(135..728)
                     /gene="rdgB"
                     /locus_tag="PKZ49_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HND37335.1"
                     /translation="MQQLIIASNNTGKLREIEQILAPLEIQILPQKDLGVTEIEEPYQ
                     TFVENALAKARHASEQTGLPALADDSGICVQSLGGAPGIFSARYAGEPKSDQRNNQKL
                     IETLKFNSDRAAYYYCIMVLIRSANDPQPIIADGYWFGEIILEPRGEGGFGYDPHFFV
                     PELGKTAAQLTSEQKNQYSHRSKALVKLTQMLVNCRF"
     gene            complement(736..1458)
                     /gene="rph"
                     /locus_tag="PKZ49_12045"
     CDS             complement(736..1458)
                     /gene="rph"
                     /locus_tag="PKZ49_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HND37336.1"
                     /translation="MQRKNNRATYQIRPISVTRRYTKYAQGSVLIECGDTKVICTASI
                     SDKVPPFLKGSGQGWLTAEYGMLPCSTHERIQREASKGKQSGRTMEIQRLIGRSLRAI
                     TNLALIGERTIQIDCDVIQADGGTRTASITGAYVALHDAIDTLLEQKIITSSPILDQV
                     AAISVGIYQGIPIIDLDYTEDSACDTDMNVVMTGNLGLVEVQGTAEGVTFNRGELNTM
                     LDMAQQGIQELISIQKSALALS"
     gene            complement(1532..>1953)
                     /locus_tag="PKZ49_12050"
     CDS             complement(1532..>1953)
                     /locus_tag="PKZ49_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HND37337.1"
                     /translation="EVTYESMWRGIEEIKPGKYLGDIGHAIQNMAEKAGYSVVREFCG
                     HGIGAKFHEEPQVLHYGRSGTGIALKAGMVFTVEPMINAGKAAIRHLPDGWTVVTKDG
                     SLSAQWEHTVLVTDTGYELLTVSAGTPPKPTSYQITT"
BASE COUNT          542 a          459 c          374 g          578 t
ORIGIN      
        1 attaaaatca caataagggc attttttgat acaccaggga aaatgaatgt acaaactgag
       61 cggaggtagc tcaagtaagt gaggtgattg aaaatttaaa actggcgcaa tcgttttggg
      121 ttcaaccata aaaactagaa acgacaattc acaagcattt gggtcagttt taccagcgcc
      181 ttcgaacgat ggctatattg attcttttgc tctgaagtta actgcgctgc agtttttcct
      241 aattctggta caaagaaatg cggatcatag ccaaatccac cctcaccccg gggctctagg
      301 attatttcac caaaccaata gccatcagcg atgattggtt gtggatcatt agcactgcga
      361 attagcacca taatgcagta ataataggct gctcgatctg aattaaattt gagtgtctca
      421 ataagctttt gattatttcg ctgatcggat ttgggctcac ctgcataacg agccgaaaag
      481 atacccggcg cccctcctaa tgattgcaca caaatccctg aatcatctgc aagtgcaggc
      541 aatcctgttt gttcactcgc atggcgtgct tttgctagtg cattttcaac aaatgtttga
      601 taaggctcct cgatttcagt cacacccaaa tctttctgtg gcagtatttg aatttcaagt
      661 ggcgccaaga tttgttcaat ttctcgtaat ttacccgtgt tattacttgc aatgattaat
      721 tgctgcatac ctttcctaac tgagcgccag cgctgatttt tgtatgctaa tcagctcttg
      781 aattccttgt tgggccatat cgagcatcgt attcaattca ccacgattaa aagttacccc
      841 ttctgcagtt ccttgtactt ctactagacc aagattccct gtcataacca cattcatatc
      901 ggtatcacaa gccgagtcct ccgtgtaatc aagatcaatg attggaatac cctgataaat
      961 ccccaccgaa atagctgcca cttgatcgag aatagggcta ctggtgataa ttttctgttc
     1021 tagcaatgta tcgatagcgt catgtaaagc aacataagca ccggtaatgc tcgctgtacg
     1081 agttccacca tctgcctgaa taacatcaca atcaatctgt atggtacgtt caccgattaa
     1141 ggctaggtta gtaatcgccc gaagtgaacg accaatcaat cgttgaatct ccatggtccg
     1201 tccagattgc ttgccctttg aagcctctcg ctgaattcgc tcgtgagtgg aacagggaag
     1261 cattccatac tctgcagtta gccaaccttg accacttcct ttaagaaacg gaggaacctt
     1321 atcagagata ctcgcggtac aaataacttt cgtatcaccg cattcaatta gtactgatcc
     1381 ttgagcatat ttcgtatagc gtcgcgttac tgatatggga cgaatttgat acgtagctcg
     1441 attattctta cgctgcatga gttatcatta ttagaattta gcaatcctct tttaacaaga
     1501 ggaaatgaag gtggattcta cattaaaacc actaagtggt tatttgatag gaagttggtt
     1561 taggtggagt ccctgctgaa accgttaaca attcatagcc tgtatcggtc actaacaccg
     1621 tatgctccca ctgtgctgac aaactaccgt cttttgtcac tacggtccaa ccatcaggca
     1681 aatggcgaat tgcagctttt ccagcattga tcatcggttc caccgtaaat accattcccg
     1741 ctttaagagc aatccctgtc cctgatcggc cataatgaag cacttgaggt tcttcatgaa
     1801 atttagcacc gatgccatgg ccacaaaatt ctctgacaac actataacca gccttttcag
     1861 ccatattttg aattgcatgg ccaatatcac ccaggtactt tccaggtttt atttcttcga
     1921 ttccccgcca catggattca taagttactt cac
//
LOCUS       DAOQKQ010000253         1864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_52537_length_1864_cov_5.656716, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000253 DAOQKQ010000000
VERSION     DAOQKQ010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52537_length_1864_cov_5.656716"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PKZ49_12055"
     CDS             complement(<1..233)
                     /locus_tag="PKZ49_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37338.1"
                     /translation="MPDGAISERAVYPAAHPDHPRHIGSYSAGMCDHILPITMPLPPA
                     SNSPFYSLHTLLQISSSWSPVATPSVSFEILLG"
     gene            125..1198
                     /locus_tag="PKZ49_12060"
     CDS             125..1198
                     /locus_tag="PKZ49_12060"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HND37339.1"
                     /translation="MGVCDRTFRRYMNRYDEGGLDALLDKRLVQISHRRALVDEVMRT
                     VDLYRDRHLGWNAKHFYAWHRKEIGTRSYTWVKSRLQEAGLIERAKKRERAPLVGMMI
                     HQDGSTHEWITGEKWDLIVTMDDATSEHYSMFLVEEEGTASSFQGVQEVIEEQGLFAS
                     FYSDRGSHYWHTPEAGGKVDKQNLTQFGQALKRLGIEMIAAYSPEARGRSERMFRTHQ
                     ERLPKELALAGITHMAAANRYLKEIYMPAFNAEFMQSASEEGSAFVPWIGGQLADILC
                     ERHERVVGHDNCVSFEGLKLQIPADRHRCHYVKARVTVLRYPNRQMAILHGPRRLATY
                     DALGKEIKLSNTVSRKSAATTAQ"
     gene            1441..1864
                     /locus_tag="PKZ49_12065"
                     /pseudo
     CDS             1441..1864
                     /locus_tag="PKZ49_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008182253.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          545 a          408 c          484 g          427 t
ORIGIN      
        1 aatccgagga ggatttcaaa tgaaacggac ggagtggcta caggagacca ggaagatgag
       61 atttgaagaa gcgtatgaag gctatagaaa ggggagtttg acgcaggcgg aagcggcatt
      121 gttattggga gtatgtgatc gcacattccg gcgctatatg aaccgatatg acgagggtgg
      181 tctggatgcg ctgctggata aacggctcgt tcagatatcg caccgtcggg cactggtgga
      241 tgaagtaatg cgaacagtag atctatatcg tgaccgccac ctgggttgga acgctaagca
      301 cttttatgcg tggcaccgga aagagatcgg tacgcgcagc tacacatggg ttaaaagccg
      361 gttgcaagaa gccgggttga ttgaacgcgc caagaagcgt gaacgtgcgc cgctggtagg
      421 aatgatgatt catcaagatg gtagtacgca tgaatggatt actggggaga aatgggattt
      481 gatcgtcacg atggatgacg ccaccagtga gcattactcg atgttcctcg ttgaggaaga
      541 aggcacagcc agcagctttc aaggtgtaca agaagtcatt gaggaacagg ggttgtttgc
      601 atcgttttac tcggatcgcg gcagtcatta ctggcacaca ccggaagcgg gcggcaaagt
      661 agacaaacag aaccttaccc agtttggcca ggccctgaag cgactgggaa tcgaaatgat
      721 cgcagcctat tcacccgaag cgcgtggacg cagtgagcgc atgtttcgca cacaccagga
      781 gcgattaccc aaggaactgg cgctagccgg tattacccat atggctgcag ccaatcgata
      841 cttgaaagaa atctatatgc ctgccttcaa tgccgagttt atgcagtcgg caagcgagga
      901 aggcagtgct tttgtaccct ggatcggtgg acagcttgcc gacatcctct gtgaacggca
      961 tgaacgcgtc gtaggccacg ataactgcgt tagttttgaa ggcctgaaac tacaaatccc
     1021 agcagatcgt caccgctgtc attatgtgaa agccagagtg actgtgctac gctatccaaa
     1081 cagacaaatg gcaatcttgc acggaccaag gaggcttgcc acctacgatg cgcttggtaa
     1141 ggaaataaaa ctaagcaaca cagtttcgcg taaatccgcc gcgacaactg cgcaatgaat
     1201 aaagtgataa ttcaatgcgg ttcaaagcgg acaatttatt tgctacaaaa ccggacagtt
     1261 ctatttgttg ctaacatcgg ggcttccgtc ttccgattgt agaaattaaa agtgctgtgg
     1321 acgaacacca ttggatacat tacttgatgg caaatcaatt tgggcagaaa aaaatttagc
     1381 tcaaatctaa actgacagac acctaaaaaa actggtaact gtcagatcag gtctaagcta
     1441 gtacaagttg atgtgaagtt tctcgatttt aaagttgaag atggtaagaa aatccggcgc
     1501 ttccaatata ccgccattga tgacgcaacg cggatccgcg cgctgaaaat ttatgagcga
     1561 catacacagc agaacgcgat tgatttcgta gattatgtta tctcaaaatt ccctttcaga
     1621 atcaatacta tccgaactga taatggccat gaattccaag ctaaattcca ttggcacctg
     1681 gaagacatgg gtgtgcgaca ttcttacatc aaacccagaa cgcctcgatt aaacggcaag
     1741 gtggaacgtt cgcatggcac tgatcaccgg gaattttacc agttattaac ttatacagat
     1801 gatgttgacc tgcatgccaa attagccgag tgggaagctt tctacaacta tcaccgacct
     1861 catg
//
LOCUS       DAOQKQ010000254         1863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_52601_length_1863_cov_7.386615, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000254 DAOQKQ010000000
VERSION     DAOQKQ010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52601_length_1863_cov_7.386615"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..243
                     /locus_tag="PKZ49_12070"
     tRNA            159..243
                     /locus_tag="PKZ49_12070"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:193..195,aa:Leu,seq:gag)
     gene            382..>1863
                     /locus_tag="PKZ49_12075"
     CDS             382..>1863
                     /locus_tag="PKZ49_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37340.1"
                     /translation="MPYLTIKALFIFLIKTLIYAGAVYYTTNQFTIDNLGTVILGVFI
                     LSLPIILCGTYGSTVRQIRRLTIFKERGILFKIVSSRFLKSILWILWALSTSFFMLLQ
                     FHTYSEWEWFFFLLVIPFFWIVFSTCRKFIAHEIVGYMATDIALRWARFLCPILMLIL
                     YYAFFGQFETLPNYANIPEAIQDQKSRVENLAGSTITREASHFLALYDGIRIFILNHS
                     LSQDTIWGFLLLTFSKLIVFFNACAILSCFVIPTTEFRRIVGPLTDQDIPPALSIQRI
                     AIASAIFTLLTLFIYAPTFVYLEAWVQQKSIALNEDRTKIEKVAVDLIGEEPFRKGTI
                     EKLHKAKIKALPDKAAIQRDLDEKLEIGFKRLESRVDIYLDWYYSLMGEYTRISKLLI
                     GELEEDMKRRFQEALEQGGNPFWEYEAAVSQLLMHYEDAQKAYIEKANKILHKNRLDL
                     KGVPFDVNEQLTLLDAVNLPIHQEDFINLQHRLGTGSAAGMITA"
BASE COUNT          499 a          398 c          410 g          556 t
ORIGIN      
        1 gcggctgcaa tcgaaagtga aaaaaaatct gctaaggatc gcaatcagag cgcccctgaa
       61 attgaaggta attcaaaggt cgaccaaata cccgattagc aatctacagt tctttcttta
      121 atatggcagc ctattggttt taaaaaaatt gttttgctgc cgacgtggtg gaattggtag
      181 acacgccgtc ttgagggggc ggtggcgaaa gctgtgcgag ttcgagtctc gccatcggca
      241 ccaatcgttt ttttctgcta tattattctc gtactgattg ttaaaaaatc agtatcacat
      301 gcgataccca aaataaacgg cttatcgtct cgatccgatc tgaatcggta gaataataat
      361 aaaaacttca atcgcttggt catgccctac ctaaccatca aagcgctttt catttttctg
      421 atcaagaccc tgatctacgc aggtgctgtc tattacacta ccaatcaatt cacgattgac
      481 aatcttggga cggttattct cggtgtcttt attctcagct tgcccattat tctgtgcggg
      541 acctatggca gtactgtgcg ccagattagg cgtctcacaa ttttcaagga gagggggatt
      601 ttatttaaga tcgtttcgtc gcgctttttg aaatcgattt tatggatact gtgggcactg
      661 agtacttctt ttttcatgtt gctgcagttt catacctata gcgagtggga atggttcttt
      721 tttttgctgg tcattccgtt cttttggatc gtatttagca cttgccgcaa gttcattgct
      781 cacgaaattg tgggctacat ggcaaccgat attgctttac gctgggctag gttcctttgc
      841 ccgatattga tgctaatcct ttattatgct ttctttggac agttcgaaac gttacccaat
      901 tacgcgaata ttcctgaagc gattcaggat caaaaaagca gggtggagaa tctagcggga
      961 agcactatta cccgcgaagc ttcgcatttc ttagcgcttt atgatggcat ccggattttt
     1021 atcctcaacc attcgctttc acaagacacg atttgggggt ttctgttgct aacatttagc
     1081 aaactcattg ttttcttcaa tgcttgcgct attctttctt gtttcgtgat tccgacgacg
     1141 gaatttcgac gcatcgttgg gccgcttact gaccaagata ttccccccgc gctgtcaatc
     1201 caacgaatcg cgatagcctc ggccattttt accctactga ctctatttat ttatgcgccc
     1261 acttttgtgt atttagaagc gtgggtgcaa cagaaatcga ttgcactgaa tgaagatagg
     1321 acaaaaatcg agaaagtcgc tgtcgatctc attggtgaag agccatttcg caagggcacc
     1381 atcgaaaaac tgcataaagc taaaatcaag gcgttgccgg ataaagcagc gattcaacgc
     1441 gacctagacg agaaactaga aatcggtttt aaacggctgg aatccagagt agacatctat
     1501 ctagattggt actatagctt gatgggtgag tacacgcgga tcagtaagct attgattggt
     1561 gaattagaag aagatatgaa acgccgattt caagaagcac tggaacaagg tggtaatcct
     1621 ttctgggaat atgaagcggc tgttagtcag ttactgatgc attatgaaga tgctcaaaaa
     1681 gcctatatcg aaaaggcaaa caaaattttg cacaaaaacc gcctcgatct caaaggtgtg
     1741 ccgtttgatg tcaatgagca actcacgctg ttggatgcag taaatttacc cattcaccaa
     1801 gaagatttta ttaatcttca gcatcgcctt ggtacaggat cagccgctgg tatgattacc
     1861 gcc
//
LOCUS       DAOQKQ010000255         1851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_53286_length_1851_cov_5.339087, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000255 DAOQKQ010000000
VERSION     DAOQKQ010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53286_length_1851_cov_5.339087"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /gene="thrC"
                     /locus_tag="PKZ49_12080"
     CDS             complement(<1..1007)
                     /gene="thrC"
                     /locus_tag="PKZ49_12080"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HND37341.1"
                     /translation="MRYISTRGGMSPQNFSEILLSGLAQDGGLAVPESYPRVDAAELE
                     SWRYLSYPELAFEILSRFMDDIPTANLRDIITRTYTSKLFGSADITPIKTLEPGLHIL
                     CLSNGPTLAFKDIAMQFLGNLFEYTLAKTGSELNILGATSGDTGSSAEYAMRGKQRIR
                     VFMLSPYGKMSRFQTAQMFSLQDENIYNIAIQGVFDDCQDIVKAVSNDHPFKKGHHIG
                     TVNSINWARIAAQIVYYFKGYFAVTQSNDEFVSFSVPSGNFGNIFAGHVARMMGLPIK
                     HLILATNENDVLDEFFRTGLYRPRTTSETHHTSSPSMDISKASNFERYVFDLSERDSA
                     R"
     gene            complement(1069..>1851)
                     /locus_tag="PKZ49_12085"
     CDS             complement(1069..>1851)
                     /locus_tag="PKZ49_12085"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HND37342.1"
                     /translation="TALATAQRLGYAEADPTYDIEGIDAAHKITIMAAIAFGIPVRFE
                     KAYIEGISQLNNEDIRNAEELGYRIKLLGITKRVEKGIELRVHPTLIPIKRLIANVEG
                     VMNAIVVKGDAVGPTLYYGAGAGAEPTASSVIADLVDVARQLTVDPKHRVPYLAFPPD
                     SLSDTPIVSMEDVQTAYYLRLQVIDKPGVLADITRILADADISISAMIQKEHAEEEDR
                     VNIIMLTHTTQEKNINRAIDKIEKLPSMTDKVVRIRLEELNK"
BASE COUNT          531 a          374 c          398 g          548 t
ORIGIN      
        1 attctagctg aatcacgctc agatagatca aatacatagc gctcaaaatt cgatgcctta
       61 gagatatcca tagaagggct gctggtgtga tgtgtttctg acgtagttcg tggacggtaa
      121 agtccagttc ggaaaaactc atctaataca tcattttcat tcgttgcaag aatcaaatgc
      181 ttgataggaa gccccatcat ccgcgcgaca tgtcctgcaa aaatattgcc aaaattacca
      241 gatggcacgg aaaatgatac gaattcatcg tttgattgcg taacagcgaa atagcctttg
      301 aaataataga caatttgagc cgcgattctt gcccagttga tcgaattgac agttccaata
      361 tgatggccct ttttgaaagg gtgatcattg ctgactgctt ttacaatgtc ttggcaatca
      421 tcgaataccc cttgaattgc aatgttatag atgttttcat cttgtaggga aaacatctgg
      481 gcagtttgga agcgactcat cttaccataa ggggagagca tgaatacccg tattctttgt
      541 tttccacgca ttgcatattc tgcgcttgag ccagtatctc ctgaggttgc tcctaggata
      601 ttgagttcac tgcctgtttt ggcgagagta tattcaaata gattgcctaa gaattgcatt
      661 gcaatatctt tgaaagcgag ggttggccca tttgataaac ataaaatgtg taatcctggt
      721 tccagtgtct taatcggtgt gatatcagca ctaccgaata gtttagatgt ataggtacga
      781 gtgatgatat ctcggagatt ggcggttgga atatcatcca tgaagcggga tagaatttca
      841 aaagcaagct ccggataact taaatagcgc cacgattcaa gctctgctgc atcgactcga
      901 ggataggatt cgggtacagc taaaccccca tcttgtgcaa gtccactcaa cagaatttca
      961 ctaaaatttt gcggcgacat accgcctctg gtagagatat aacgcatagt gtatgaccga
     1021 tctagaaaag agggatggga aaagatgatg ctattttccc aagttaattt atttgtttaa
     1081 ttcttctaaa cgaatacgca cgactttatc tgtcatgcta ggcagttttt caatcttatc
     1141 gattgctcta ttgatattct tttcttgagt agtatgagtt agcataatga tgttaactcg
     1201 atcttcttcc tcagcatgtt ctttttgtat catagcacta atcgagatat ccgcatcggc
     1261 aaggatacgg gtgatatcag ctagcacacc aggcttatca atgacttgaa gccttaaata
     1321 gtatgcggtt tgtacatctt ccattgagac aatgggggta tcagaaagtg aatcgggagg
     1381 gaaggccaaa tagggaacac ggtgcttggg atctaccgta agttgtcgag caacatcgac
     1441 caaatcagca atcactgaac tggcagtggg ttctgcacca gcgccagcac cataatataa
     1501 agttgggcct accgcatctc ctttaaccac aatggcattc atgacgcctt cgacattagc
     1561 aatcaaacgt ttgataggaa ttaatgtagg atgaactcgt aattcgattc ctttctctac
     1621 ccgcttggta atgcctaata atttgattcg atagcctaat tcttcagcat tgcggatatc
     1681 ttcattattt aattgggaga taccttcaat ataagctttt tcaaatcgaa cagggatacc
     1741 aaacgcaata gcagccatga tggtaatttt atgggctgca tcgatccctt caatatcata
     1801 agtcggatct gcttccgcgt aaccaagtcg ttgtgcagtg gcaagtgcag t
//
LOCUS       DAOQKQ010000256         1818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_55045_length_1818_cov_4.623936, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000256 DAOQKQ010000000
VERSION     DAOQKQ010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55045_length_1818_cov_4.623936"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PKZ49_12090"
     CDS             complement(<1..661)
                     /locus_tag="PKZ49_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HND37343.1"
                     /translation="MVESQEPRMPRSIDFWDAFRFWLKLGFISFGGPAGQIAIMHREL
                     VENRRWISEQRFLHALNYCMLLPGPEAQQLSVYIGWLLHRTWGGIVAGLLFILPSLFI
                     LIVLTWIYLAFQDAPVVLGIFYGIKPAVTAIVIHAAYRIGSRTLKNGLLWAIACAAFI
                     AIFALKIPFPIIVAIAALIGYLGGRFSSDYFKLGSHPTKQMNQSFGPAVIDDDSPRLS
                     HT"
     gene            complement(784..>1818)
                     /locus_tag="PKZ49_12095"
     CDS             complement(784..>1818)
                     /locus_tag="PKZ49_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03032 family protein"
                     /protein_id="HND37344.1"
                     /translation="TKAPILEIDLSRQFTAWLAEQNLSIAFTTYQVGKLCLIGLNANR
                     ELSVSERSFNRCMGLCTTGNGFYMSSLYQIWRFENMLESGQRQSGYDRLYFPQLGYTT
                     GDLDVHDMAIDKQGTLIFVNTLFSCLATLSNTHSFKPIWKPSFISKLAAEDRCHLNGL
                     AMQNGLPAYVTAIGQSDMVDGWRSHRMGGGIVIDVRSNEIICDGLSMPHSPRWHQEKL
                     WLLNSGTGEFGFVDPKIGRFESLCFCPGYARGLSFHGNYALIGLSKPRTDKTFKDLPL
                     DAQFKKRNVEPRCGILIINLCSGDIVHWFRMDGIVTELYDVITLPNVNQPMALGFKTD
                     EIRRMISIEQ"
BASE COUNT          572 a          423 c          354 g          469 t
ORIGIN      
        1 aggtatgcga taaccgtggc gaatcatcat cgataactgc tggtccaaaa gattgattca
       61 tttgctttgt aggatgacta cctaacttaa agtaatcaga actgaaacgc cctcccaaat
      121 atccaatcaa cgctgctatg gctacgatga ttggaaatgg tattttaagt gcaaagatag
      181 caatgaaagc tgcgcatgca attgcccaaa gcagcccatt tttgagcgta cgcgatccaa
      241 tccggtacgc tgcatggata acgatcgcgg taacagccgg ctttatccca taaaaaatac
      301 ctaaaacaac gggagcatcc tggaatgcaa gataaatcca cgtgaggaca attaaaatga
      361 acaaggaggg cagaataaag agcaatcctg caacaattcc accccatgta cggtgcaaca
      421 accagccgat atagactgaa agttgctgtg cttcaggtcc aggtaatagc atgcaataat
      481 ttaatgcatg caagaagcgc tgttcagaaa tccatcgacg attttcgaca agctcgcgat
      541 gcattatggc aatttgcccc gcagggccac caaagctgat aaatcctagt tttaaccaaa
      601 accgaaaggc atcccagaaa tcaatcgacc gaggcattct cggttcttgt gattcaacca
      661 tatcgatacc cttccccatt tgccatttaa cttcaaaaag gcattagtct atatcgataa
      721 tgatattact tgggggtttt tgagaaattg atcgggatta gtatgacaag ccatccatac
      781 ttcttattgt tcgatactga tcatgcgccg aatttcatca gtcttaaaac ctagcgccat
      841 cggctggttc acattgggta aggtaatcac atcatataat tcggttacaa ttccatccat
      901 gcggaaccag tgcacaatat cgccactaca caaattaatg atcagaatac cgcaacgcgg
      961 ctccacattt cgctttttaa attgagcatc taatggtaaa tctttgaaag tcttatcagt
     1021 tcttggcttg gataaaccta tcaatgcata attgccatga aaactcaaac ctcgcgcata
     1081 accagggcag aaacataaag attcaaaacg tcctatcttg ggatcaacga aaccaaattc
     1141 accggttcct gagttcagta accaaagctt ctcttggtgc cagcgtggag aatgaggcat
     1201 cgataatccg tcgcaaataa tttcattact gcgtacatcg atcacaatgc ctcctcccat
     1261 acgatggctg cgccagccat ctaccatatc ggactgaccg atggctgtca cataggcagg
     1321 cagcccattt tgcatcgcta aaccattcaa gtggcaacga tcttctgctg ccaacttact
     1381 aataaaacta ggtttccaga taggtttgaa gctatgcgtg ttacttaagg ttgccaagca
     1441 actaaacaac gtattaacga atatgagcgt tccttgttta tcaattgcca tatcatgaac
     1501 atcaaggtca ccggtggtat aacccaattg aggaaaatat aaccgatcat acccgctttg
     1561 tcgctgaccg gattcaagca tgttctcgaa tcgccaaatt tgataaaggc tactcatata
     1621 aaatccatta ccagtcgtac acagtcccat acagcggttg aaactgcgtt cagaaactga
     1681 aagttcccga ttagcgttaa gtccaatcaa acaaagttta cctacctgat aggtggtaaa
     1741 tgcaatcgag agattctgct cagctaacca tgcagtaaat tgacgagaca agtcaatttc
     1801 gagaattggt gcttttgt
//
LOCUS       DAOQKQ010000257         1752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_59010_length_1752_cov_6.074249, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000257 DAOQKQ010000000
VERSION     DAOQKQ010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59010_length_1752_cov_6.074249"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..1723
                     /locus_tag="PKZ49_12100"
     CDS             167..1723
                     /locus_tag="PKZ49_12100"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HND37345.1"
                     /translation="MIVSFFLHKSHGIYTSGKQSLSLAAFVLVFTSTFCFAETGLCKS
                     TEAALGQTIELTERSVKPSCLPNRDFTRLGIMDLAGKATLLPHCRVIGRNAHGWIAVR
                     MLNPERAVRKLIGPHGQIIPKTNNYEIVNYLTEKRAPVRVFPRTYDKNGMENLAFSYC
                     TETGDILAARFDQVSAFSNGVAAVRRGRLVGFVGLDGKYLANSKLVECEFKDQLSQGC
                     LAACVNGRWGYLDTGGNWIIKPQFDRAEPFRNDFALVVLANSGSAVEKSNCVSYVDKT
                     GRIFDQRFHCGESFEEGYAAVSVVSMAQPSTRLWGLIDGRGEWVLEPRYTRIDPPVGS
                     KRLVHNNDLVGIFSNGKLTLAPKYQHIGNFCDGLASFRESENGKVGFLNPEGRVIIPA
                     RFEFAFDFSEGLAAVGYPPIKGTDKSSLGFVDRKGQLIMTPKSGGNPAFSARYSPSEI
                     QFHEGVCLIPYLSNPKDRFKINRFIFINRAGQRLNNVEILEGSAFIVGRALIRYYPKS
                     RSCLSGIKAI"
BASE COUNT          478 a          369 c          426 g          479 t
ORIGIN      
        1 gtgccgatgt caacttgata attcagcaaa tagctgccta tcagcaagat accggcgcca
       61 gcatcaccaa tgttaatgat gtccgcaata accaagacct catgacgata gtcgccaaca
      121 actggcacgc ggcgtagttg aagggcgagc agtgaggagc ttaacgatga ttgtcagttt
      181 ttttcttcat aaaagtcatg gtatctacac aagcgggaag cagagcctga gtctcgcagc
      241 atttgtcctc gtgtttacat caacattctg ttttgcagaa acaggtctct gtaaatctac
      301 tgaagctgca cttggacaaa ctattgaact gaccgagcgt tctgtgaaac ctagttgtct
      361 accgaatcgt gattttactc gactgggcat aatggatctt gcaggaaaag caaccctcct
      421 tccgcattgt cgtgtcattg gtcgcaatgc ccatggctgg atagcagtca ggatgctgaa
      481 ccccgaacga gcagtgagga agctcattgg gccccatggt cagataatcc caaagaccaa
      541 taactatgag atagtaaatt acctgacaga gaagcgtgcc cctgtgcggg tctttccacg
      601 aacttatgac aaaaatggta tggagaacct tgccttctcg tattgcactg agactgggga
      661 tattcttgcc gcaagattcg atcaggtcag tgcttttagt aatggagtgg ccgctgtgag
      721 acgcggtaga cttgtgggtt ttgtaggtct cgatgggaaa tatcttgcaa attctaagtt
      781 agttgaatgc gagtttaaag accaattgtc tcaaggatgc ttggccgcat gtgtgaacgg
      841 gcgctggggt tatctggata ccgggggcaa ttggatcatt aagccacaat ttgatcgcgc
      901 cgagcctttc agaaacgatt ttgctttggt cgtactggcc aattcaggat cggctgtaga
      961 aaaatctaac tgtgttagct atgttgacaa aactggcaga atatttgatc agcgttttca
     1021 ttgtggcgag tcatttgaag agggatatgc ggctgtctca gttgtatcaa tggcgcaacc
     1081 ttctactcgt ctctgggggt taatagatgg ccggggggaa tgggttcttg agccaagata
     1141 caccaggatt gacccgcctg taggttccaa aaggcttgtg cataacaatg accttgttgg
     1201 catattctca aatggaaaac ttacattggc gcccaaatac caacacattg gcaacttttg
     1261 cgacggacta gcctctttcc gggaatctga aaacggcaag gtgggtttcc tcaatccaga
     1321 aggcagggta attatccctg cgagattcga attcgcattc gacttttcgg aaggtttagc
     1381 tgctgttgga tatccaccta ttaaaggcac cgataaatca agtctaggat ttgttgatag
     1441 aaagggccaa ttgatcatga ctccaaagtc tggtggaaat ccagcattta gtgcaaggta
     1501 cagtccatcc gaaatacaat tccatgaggg agtttgcctg attccatatc tttcaaatcc
     1561 taaagatcgt ttcaaaatca acagatttat tttcataaat agggctggcc agcggctgaa
     1621 caatgttgaa atactagaag gttctgcctt tatagttgga cgagcattaa ttcgttacta
     1681 tccaaaatct cggtcttgtc tatcaggaat taaagcaatt tgaccgagca attgctgaac
     1741 atcaattggc at
//
LOCUS       DAOQKQ010000258         1743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_59639_length_1743_cov_5.301540, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000258 DAOQKQ010000000
VERSION     DAOQKQ010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59639_length_1743_cov_5.301540"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PKZ49_12105"
     CDS             complement(<1..246)
                     /locus_tag="PKZ49_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37346.1"
                     /translation="MLTSFLNLTVEGVSLSMSPTEIQAKLQAMGYAQVSHTTFVKERP
                     LAGGRQVIYRIEIEHTPNSRKLGYFRSESGGRNKSPVT"
     gene            complement(438..1157)
                     /locus_tag="PKZ49_12110"
     CDS             complement(438..1157)
                     /locus_tag="PKZ49_12110"
                     /inference="COORDINATES: protein motif:HMM:NF022633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HND37347.1"
                     /translation="MIVYHFISPESALKALRDRRLKMTRLNELNDLHELCIANLFDSD
                     TKRELEVFKNRVNAQYGIICFSAIYHDPVLWSHYADSHRGVALVFDIPDHKAITINYQ
                     PEQYKQIINTAIHCGEFESFGLSKLIATKFSSWHYEQEVRMICDLNDHFCQIDAKGNK
                     AYFESLSLEPFGKDALKLVGLIRGVRCDLNPMDIAGELLAADTLSIQDASIDSSTFQI
                     TSSNAYPIHGVRSFSACCFRV"
     gene            complement(1242..>1743)
                     /locus_tag="PKZ49_12115"
     CDS             complement(1242..>1743)
                     /locus_tag="PKZ49_12115"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HND37348.1"
                     /translation="RLIFWAQKLKYGVTLEPAWYWGRSPKLLYGLQVFYRCLDRKQSP
                     LNRRLRALVSNRIAQINTCAFCMDISTSVLQHQAVTFEMLGILADYESNSNFSESERA
                     ALAYAEAMTDSAQRVNDVLFQRLSQSFSEEQIVELTAWIAFQNLSSKFNAALDIPAQG
                     FCQTPK"
BASE COUNT          574 a          377 c          311 g          481 t
ORIGIN      
        1 cgtcacggga ctcttgttcc ttccaccact ttctgagcga aaataaccca atttacgact
       61 gttaggcgta tgttcaattt caatgcgata aatcacttgc cgtccccctg cgagtggtct
      121 ttctttaaca aaagtcgtgt gactcacttg agcataaccc attgcttgta acttagcttg
      181 gatttcagtc ggtgacatac tgagagaaac cccttcaaca gttaaattaa gaaaactcgt
      241 cagcaaatcc gagtcaacat tcgactggtt atttaaagca aaggagcttg atgaatgtat
      301 aaataaaaat acaactaaga gtatgattcg ggaggtaata tgcgtaaaaa aacaatgaga
      361 aaacatgaaa tttacctcct gattagtaac aaatcttcaa ttcgacaacg ctatcaattc
      421 tctcgatctc atcacggtca gaccctaaag caacaagcgg agaatgaccg cacgccatgg
      481 attggataag cgttactaga tgtaatctgg aaggtcgaag aatctatact cgcatcttgg
      541 atagaaagcg tatcggccgc gagtaactca cctgctatat ccatggggtt caaatcacat
      601 cgaacacccc ttatcaatcc aactaatttt aaagcgtcct taccgaatgg ttccaaactg
      661 agcgattcaa aataagcttt atttcctttt gcatcgattt gacaaaaatg atcgttcaaa
      721 tcacagatca tccgaacctc ttgttcataa tgccagcttg aaaattttgt ggctattaat
      781 ttactcaaac caaaagattc aaactcacca caatgaatcg cagtattgat gatttgttta
      841 tattgctcag gctgataatt gattgtaatt gccttatgat caggaatatc aaacactaga
      901 gcaacgccac gatggctatc agcatagtga ctccatagaa caggatcatg gtaaatcgcc
      961 gagaaacaaa taattccata ttgggcattc actcgattct tgaaaacttc taactcacgt
     1021 ttggtatccg aatcaaacaa attagcaata cacaattcgt gcaaatcatt tagctcattg
     1081 agacgtgtca ttttcagacg acgatcacgt aatgctttaa gcgccgactc aggcgaaata
     1141 aaatgataaa caatcatgaa aaattacttg aaatattctc ttttaaatag cttttacaaa
     1201 aaaaatccag cctaacacta ctaatatatc gatttattga atcacttcgg tgtttgacaa
     1261 aacccttgcg cagggatatc aagtgccgca ttaaatttac ttgaaagatt ctggaaagca
     1321 atccaagcgg tgagctctac gatctgttct tcagaaaagg attgactcaa acgctgaaaa
     1381 agaacgtcat tcacacgttg ggctgaatcc gtcattgcct cagcgtaggc caaagcagct
     1441 cgctctgatt ctgaaaagtt agaatttgat tcgtaatcag ccaatatacc cagcatttca
     1501 aatgttaccg cttgatgttg caagactgag gtactgatat ccatacaaaa tgcacaagta
     1561 ttgatttgtg caatccgatt tgaaaccaat gcccgtaatc gtcgattcaa cggagattgc
     1621 ttgcgatcta agcagcgata aaaaacttgt agaccataca gcaatttcgg tgaacgcccc
     1681 caataccatg ctggctcaag cgttacacca tatttcagtt tctgtgccca aaaaattaag
     1741 cgg
//
LOCUS       DAOQKQ010000259         1733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_60287_length_1733_cov_6.213945, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000259 DAOQKQ010000000
VERSION     DAOQKQ010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60287_length_1733_cov_6.213945"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PKZ49_12120"
     CDS             complement(<1..270)
                     /locus_tag="PKZ49_12120"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HND37349.1"
                     /translation="MKFQLLQTEGRARRGSLTLAHGTVETPVFMPVGTYGAVKTVSPA
                     ELQELQAQIILGNTFHLWLRPGLEVIEAHGGLHQFMQWHGPILTDS"
     gene            complement(267..1301)
                     /gene="queA"
                     /locus_tag="PKZ49_12125"
     CDS             complement(267..1301)
                     /gene="queA"
                     /locus_tag="PKZ49_12125"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HND37350.1"
                     /translation="MKIQDFDFFLPAELIAQFPSERRTDSRLLCLDGPSGQYRDALFT
                     DLPNFLRPGDIVVLNNTQVIKARLFGTKETGGSIEVLVERILDEQHALVMIRASHAPK
                     IGSKLILADQISAIVNDRQQGFYLLQFEHALSVLQLLAQYGQLPLPPYIERDATLADE
                     DRYQTVFASEAGAIAAPTAGLHFDENLLRQLRELGVNIAYITLHVGSGTFQPVRTENV
                     LEHIMHSELYHIPEETTEAIQQAKRKGGRVLAIGSTTLRTLESCAQKHNGEIVPGHGS
                     TQLFITPGFKFQIVDRLLTNFHLPRSTLIMMISAFAGLENIRRAYCHAITQRYRFFSY
                     GDAMLIERKI"
     gene            1496..1572
                     /locus_tag="PKZ49_12130"
     tRNA            1496..1572
                     /locus_tag="PKZ49_12130"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1530..1532,aa:Arg,seq:acg)
     gene            1662..1733
                     /locus_tag="PKZ49_12135"
     tRNA            1662..1733
                     /locus_tag="PKZ49_12135"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1695..1697,aa:Phe,seq:gaa)
BASE COUNT          494 a          388 c          378 g          473 t
ORIGIN      
        1 cgaatctgtc aatatcggac catgccattg cataaattga tgtagcccac catgcgcttc
       61 aatgacttcg agaccaggac gtaaccataa atggaaggta tttcctaata taatttgcgc
      121 ttggagttcc tgaagttcag ccggtgaaac cgttttgact gcaccgtaag tgccaactgg
      181 cataaaaaca ggcgtttcta ctgtgccatg cgcaagtgtc aacgatcctc gccttgccct
      241 accctcggtt tgaagcaact gaaacttcat attttccttt ctattaacat ggcatcacca
      301 taactaaaaa aacgataccg ttgtgtaatc gcatggcaat aagcacgacg aatgttctca
      361 agacctgcaa atgcagaaat catcatgatt agcgttgaac gtggtagatg aaaattagtc
      421 agcaaacgat caacgatctg gaatttaaaa ccaggtgtga taaaaagctg agtgctgccg
      481 tgacctggaa caatttctcc attgtgcttt tgcgcacagg actcaagtgt tctcaatgtt
      541 gtgctaccga tcgctaaaac tcgccccccc ttgcgctttg cttgctgaat ggcttccgtg
      601 gtttcttcgg gaatatggta cagctcgctg tgcataatat gctccagaac attctcagtt
      661 cgaacaggtt gaaatgtccc tgaacccaca tggagtgtaa tataagcaat attcacgcct
      721 aattcacgta attgcctaag caaattttca tcgaagtgca gtccagcagt gggtgccgca
      781 attgcacctg cctcacttgc aaaaacggtt tgatatcgat cttcatccgc taaggtcgca
      841 tcgcgttcaa tgtaaggggg aagcggcaat tgtccgtatt gcgctagcaa ctgcaatact
      901 gaaagtgcgt gctcgaactg gagtaaatag aagccttgct gacggtcatt aacgatcgca
      961 ctgatttgat cagccaagat taactttgaa ccaatcttag gcgcatgact cgctcgtatc
     1021 atcacgagtg catgctgttc atcgagtatt ctctcaacaa gtacttcaat gctaccacct
     1081 gtttctttag ttccaaaaag tcttgctttg atgacctggg tattattaag aaccacaata
     1141 tcaccaggcc gcaaaaaatt gggcaaatca gtaaacagcg catcgcgata ttggcccgaa
     1201 ggaccatcaa ggcaaagcaa acggctgtca gtgcggcgct cagatggaaa ttgcgcgatt
     1261 aattcagccg gtaaaaaaaa atcaaaatcc tgtattttca tcccgcttat tatactgttc
     1321 ctacgctact ccttgacaaa tgtcagcgaa tgtaacagga ttgcgtgctt gctttgagag
     1381 atctttgttt gaaaaaaaat gaggtaactt aaatcgattg cactcgcgct tagtgaaata
     1441 ggtttgatat aatacgtgca atcgtggtta acaatactgc aactcgatta taattgcgcc
     1501 cgtagctcag ttggatagag tacttggcta cgaaccaagg ggtcgtgggt tcgaatcctg
     1561 ccgggcgcgc catagattag tcagtataat tgtaggcgtt agtaaatact atttttgttt
     1621 tgggaactta tttctgaaag caaaaataaa taatccaaat aggccaagta gctcagtcgg
     1681 tagagcagag gactgaaaat ccttgtgtcg gtggttcgat tccgcccttg gcc
//
LOCUS       DAOQKQ010000260         1707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_62064_length_1707_cov_4.532082, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000260 DAOQKQ010000000
VERSION     DAOQKQ010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62064_length_1707_cov_4.532082"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="PKZ49_12140"
     CDS             complement(<1..635)
                     /locus_tag="PKZ49_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HND37351.1"
                     /translation="MDDKPKRTWDEAAYKWLMETEHKASHQQDMKQVAWIQPYFRGRF
                     LSELTREVIAEVGEIKRKETSPATANRYLATIRAILRRAALDWEWIDKPPVIKLYREA
                     KRRVRYLNATQASILIRELPPHLADMVTFSLATGLRRSNVTKLEWSQVDMQRNVAWIH
                     GDQAKAGKPIHVTLNATAIAVLTRQIGKHPQSVFSYKGKPITQVNTKAWYKA"
     gene            complement(662..991)
                     /locus_tag="PKZ49_12145"
     CDS             complement(662..991)
                     /locus_tag="PKZ49_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HND37352.1"
                     /translation="MKTLNLTEAAQFLKLHPEEVRRRAKAGIIPGAKLGKRWAFIEDD
                     LADHIRSLYACTRQALQVGHEEKKLCHSLNVVKRGGFDSLHQAASALDALLQQKIKPK
                     PKNSTTG"
     gene            complement(1017..1445)
                     /locus_tag="PKZ49_12150"
     CDS             complement(1017..1445)
                     /locus_tag="PKZ49_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraM"
                     /protein_id="HND37353.1"
                     /translation="MSNNRLDALIERVASEHGIVLSQDDPVLMMHTLNEVLLEQNEKA
                     HTELLSNYRAILEENFNRWCEFSTKKSNAIISASMNNAQLTRDQFLESCIQLIDERIK
                     SSINQETYDLVRISRQMAIMNLLAAVLVLVSVVIVVFMLL"
     gene            complement(1442..>1707)
                     /locus_tag="PKZ49_12155"
     CDS             complement(1442..>1707)
                     /locus_tag="PKZ49_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraL"
                     /protein_id="HND37354.1"
                     /translation="GEISMNGKQFEEMKAYVDNKTRVSAIIRIPHYKAETFGKDLSEI
                     LQSKFTFDEALKNSALPVMVRQRLTIIRRQLFTNIENLAAVLT"
BASE COUNT          425 a          393 c          339 g          550 t
ORIGIN      
        1 gccttatacc aagccttggt atttacttgc gtgattggct tgcccttgta actaaatact
       61 gattgaggat gctttccaat ctgccgagtt aataccgcta ttgccgtggc attcagtgtg
      121 acatgaatcg gttttcctgc tttggcctga tcaccatgta tccatgcaac atttctttgc
      181 atatccactt gcgaccattc cagctttgta acgtttgatc gtctcaaacc agtcgctagt
      241 gaaaacgtca ccatgtccgc tagatgcggt ggtaattccc gaatgagaat gcttgcttgt
      301 gtcgcattca agtaacgcac tcgacgtttt gcctcacgat acaacttaat gactggaggt
      361 ttatcgatcc attcccaatc taatgcagcc cgccgaagaa tcgcccgaat tgttgccagg
      421 tagcgattag ccgttgcagg tgaagtttcc ttacgtttaa tttcacccac ttcagcaatc
      481 acttctcggg ttaattcgga aaggaaacgg cccctaaaat agggctgaat ccaggcaact
      541 tgcttcatat cctgctgatg tgaagcttta tgttctgttt ccattaacca tttataggcg
      601 gcctcatccc aagtccgctt gggcttatca tccaatttag taactcgcca cgattcagct
      661 tttagcctgt cgtggaattc ttgggcttgg gttttatctt ctgttgcagc agagcatcta
      721 atgcgctcgc cgcttggtgt agtgaatcga atccaccacg ttttaccacg tttaaagagt
      781 gacataattt tttctcctca tgtcccactt gcaacgcttg ccgggtacaa gcatataacg
      841 agcggatatg atcggcaagg tcatcttcaa taaatgccca gcgttttcct aatttggcac
      901 cgggaatgat tccggctttg gcgcgacggc gcacttcttc gggatgcaat ttcagaaact
      961 gggctgcttc ggtgaggttt aaagttttca taagctatac aaccttttag tagaagctat
     1021 aacagcatga atacaacaat taccacggaa actagaacca gaacggcagc caataaattc
     1081 atgatagcca tttgcctgga aattctgact agatcataag tttcctgatt tatactgctc
     1141 ttaattcttt catcgataag ctgaatacag ctttccagga attggtctcg tgttaattgc
     1201 gcgttattca ttgatgcact gatgatggca tttgattttt tggtagagaa ctcacaccat
     1261 cgattaaagt tttcttctaa tatcgcccgg taattgctta gcagctcagt atgggctttc
     1321 tcattttgtt ccagtaatac ctcatttaag gtatgcatca tcaatactgg atcatcttgc
     1381 gacagaacta ttccatgttc tgaagctact cgttcaatga gcgcatctaa tctgttattg
     1441 ctcatgttaa aaccgcagct aaattttcaa tgttggtgaa gagctgcctg cggataatcg
     1501 ttaacctttg acgcaccatc accggtaatg cgctattttt aagcgcttca tcaaaggtaa
     1561 attttgattg caagatttca cttaaatctt taccaaaagt ttctgcctta taatgtggaa
     1621 ttctgatgat agctgatact cgggttttgt tatctacgta cgctttcatt tcctcaaatt
     1681 gtttgccatt catactgatt tctcccc
//
LOCUS       DAOQKQ010000261         1690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_63244_length_1690_cov_5.114985, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000261 DAOQKQ010000000
VERSION     DAOQKQ010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63244_length_1690_cov_5.114985"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..1253)
                     /locus_tag="PKZ49_12160"
     CDS             complement(114..1253)
                     /locus_tag="PKZ49_12160"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate phosphodiesterase"
                     /protein_id="HND37355.1"
                     /translation="MSALIHLIYCSAATHPMNESELRKLLVKARAKNARLGITGILLY
                     ENGSFFQVLEGTPEVIDRLFNEICQDNRHNKVSTIIRESIFKRAFSEWTMGYSSVSLQ
                     ELDEIIGLNDFFTEGSSLTEITPGRAKKILAAFRDGRWRAKVQYTSSPESEAHTKQLI
                     MQPATMVTPKVSFSFQPMINAITGTVGAYEAILRGQHDEAFPEISQQIGDAEWTYFDT
                     NCRAIAISMAAKLGLPCDLHLSFVARRVEDAREAISASLDAAEKNNIEPSRIILQIDQ
                     DRLIGERDHFAQIIEEYRGAGLRISIDHFGLGQASFNLLELLRPEMISLNAQLVRDIN
                     NSGPRQAIVLGLKQTCTDLGINLVAKHIETVAELGWFVSEGIELL"
BASE COUNT          492 a          353 c          353 g          492 t
ORIGIN      
        1 ggctggaagg caaacgagaa ttgtggaaac gacactttgg attccatacg ctacaccaaa
       61 ggccagctta tgttaggcaa gcgttcaaag gccggactag cgataacatt gccttacaat
      121 aattcaatgc cttcgctaac aaaccatcct aattcagcta ccgtttcaat atgttttgcg
      181 accagattga tacccagatc agtgcaggtt tgtttcaaac ctagcacaat ggcttggcgc
      241 ggtccgctat tgttgatatc ccgaactagt tgcgcattca acgaaatcat ttctgggcgc
      301 agcagttcca acaaattaaa actagcctgg cctaaaccaa aatgatcaat cgaaatacgt
      361 agacctgctc cgcgatattc ctcgatgatt tgcgcaaaat gatcacgttc acctatcaat
      421 ctatcttgat caatctgcaa gataatacgg ctgggttcga tgttattttt ctctgcagca
      481 tccagagagg ccgaaattgc ttcacgcgca tcctcgactc gccgtgcaac aaaacttaga
      541 tgtaaatcac acggtaaacc caatttagct gccatactaa tggcaatagc gcgacaattc
      601 gtgtcgaaat aagtccactc agcatctccg atttgttggg aaatttcagg aaaggcttca
      661 tcatgctgtc cacgcagaat ggcttcgtaa gcgcctacgg ttccagtgat tgcattaatc
      721 ataggttgaa aagaaaagga gactttggga gtaaccatgg tagctggctg catgattaac
      781 tgtttggtat gcgcttcaga ttctggactc gatgtatact gtactttagc tctccaacgt
      841 ccatctcgaa acgcagctag aatttttttt gcgcgacccg gtgtaatttc tgtaaggctg
      901 gagccttccg tgaaaaaatc attaaggcca ataatttcat ctagttcttg caaagaaaca
      961 ctcgaatagc ccatggtcca ttcactaaaa gcacgtttga aaatggattc acgaataatg
     1021 gtagacactt tgttgtgccg attatcttgg caaatttcat taaatagtcg atcaataact
     1081 tctggcgtgc cttctaatac ttggaagaaa ctaccatttt catagagaag gattcctgtg
     1141 ataccgagtc gtgcattttt tgctctcgct tttaccagta gcttgcgtaa ctcagactca
     1201 ttcattgggt gagttgcggc gctacaataa atcaggtgta tcaaagcact cattggctat
     1261 ccttaatgtc ctgttttaaa atagtgcgta atggcgctaa catgctagac aatcagcaac
     1321 agagttcaaa gtgtggaagg aagcaataag cgaaaggtta attatcaaat agaccatggt
     1381 tgctcacttt gcttttgttg aaaagcattt caaaactggc ctatgagata ggagaaaacg
     1441 ctgagctcac tgattggcgt ctggcgtttg actaagatgc gaattttatc cactggggta
     1501 atattgctaa gcatatgggg acacctatca attttcacgt tatacccttg ttttataaaa
     1561 ttttcgatcg attgggtgat catgtgatca tgatatcagg tctatttaat ttaatgccta
     1621 tgaactattc tcagatgttt ttcatcttat atagcaagat caataggata atgaggtagg
     1681 tgatgaaagg
//
LOCUS       DAOQKQ010000262         1667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_64979_length_1667_cov_4.383995, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000262 DAOQKQ010000000
VERSION     DAOQKQ010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64979_length_1667_cov_4.383995"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..650)
                     /locus_tag="PKZ49_12165"
     CDS             complement(195..650)
                     /locus_tag="PKZ49_12165"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HND37356.1"
                     /translation="MKAYAVGINHVALEVGNIHEALKFYSDFLSFEVHQENEMQAFIY
                     FGDQFLNFIKNDNRQPDQMRHFGIAVDNKELVRQTLEGMGIKLLNGPFLDFLDPWGNR
                     VEITTYSNIQYTKADHVLLGMGLGHLKKTDQALAELNAKGLGPIQYDNN"
     gene            complement(786..1565)
                     /locus_tag="PKZ49_12170"
     CDS             complement(786..1565)
                     /locus_tag="PKZ49_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HND37357.1"
                     /translation="MDQVTESAGRGLARFLTKPVRQSTQVAMISQEKLAAALQPGDVL
                     LVEGNTRISVAIKYLTQSTWSHAALYIGDALPSSMSATTPVLVEADLEEGVRAITLAH
                     YAQMHTRICRPVGLNDEDRRRVVDYAIARIGQQYDLKNIFDLVRYLLPIAPVPTRFRR
                     RLLALGSGDPTRAICSTLIAQAFQSVHYPILPEIQRAWLDDPMCADCYAEIYRIRHHS
                     LFTPRDFDISPFFNIIKPTIENGFDYHSLAWSNAIQEPGKM"
BASE COUNT          468 a          369 c          322 g          508 t
ORIGIN      
        1 gatgacatta tgctgaaaac tactcatggg attactcctt tacgctttcg cgtttggttg
       61 ataaagattc gcacctctat caaaccggta atcccaccct ttggcaagac cttattgtca
      121 gatcaaatct gaactactac aaattattac catttaatta ttactgtttc tatctgtaat
      181 tggaattagg tcaattaatt attatcatat tgaataggtc ctaaaccttt tgcattcaat
      241 tctgccagtg cttgatcagt ttttttaaga tggcccaatc ccattcccag caatacatgg
      301 tcagcttttg tatattgaat attgctatag gtggttattt ctacccgatt tccccaagga
      361 tcaagaaaat caagaaaagg gccatttaaa agtttgatac ccatcccctc aagtgtctgc
      421 ctcactaatt ctttattgtc taccgctatt ccaaagtgtc gcatttgatc aggttgtcta
      481 ttatcatttt ttataaaatt gagaaattga tccccaaaat aaataaatgc ttgcatctca
      541 ttttcttgat gaacttcaaa gctcaagaaa tcactataaa attttaacgc ttcgtgaata
      601 tttccaactt ctaatgccac atgatttatg cctactgcat atgctttcat attttttcct
      661 ttgtcattga aattcaatct aacccaaata atctattctt gtgctattga agataatatt
      721 gaagaaagtt aattaagcaa ttaatgcata aatagctaat ctgcattagt cttcattgat
      781 ttgtatcaca tcttccctgg ctcttgtatg gcgttactcc aggcaagcga atggtagtca
      841 aagccatttt cgatggttgg tttgataatg ttgaagaagg gagaaatgtc gaaatcccga
      901 ggagtgaaca aactatgatg gcggattcga tagatttccg cataacaatc agcgcacatc
      961 ggatcgtcta accaggcgcg ctgaatctcc ggcaagatcg gatagtgcac tgactgaaac
     1021 gcttgcgcaa tcaaggtcga acagattgca cgagttggat ctccgctacc gagggccaac
     1081 aggcgtctgc ggaatcttgt tggaacgggt gctatgggaa gaagatagcg tacgagatcg
     1141 aaaatgttct tgagatcata ctgttgaccg atacgtgcaa tggcataatc cacaacgcgc
     1201 ctacgatctt catcattcaa accaacaggg cggcagattc gggtgtgcat ttgcgcataa
     1261 tgagcaagcg ttattgcacg cacgccttcc tccaggtccg cttcaaccag taccggcgta
     1321 gttgccgaca tgctggaagg aagtgcatcg ccgatataca gtgctgcatg tgaccaggtc
     1381 gattgcgtaa gatatttgat ggcaacacta atacgggtat ttccttcaac caataaaaca
     1441 tctccgggtt gtaaagctgc cgcgagtttt tcctggctga tcatggcgac ctgagtgctt
     1501 tgccggactg gcttggttaa aaatcgtgcc agaccacggc ctgcactttc agttacctga
     1561 tccacaaatt cctcttttta tcgctagttc cggatttttg ttcactataa tttttactca
     1621 actttagaca tccagcccca accttgcgtc acccgaacga gacacaa
//
LOCUS       DAOQKQ010000263         1639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_67131_length_1639_cov_6.414141, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000263 DAOQKQ010000000
VERSION     DAOQKQ010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67131_length_1639_cov_6.414141"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PKZ49_12175"
     CDS             <1..307
                     /locus_tag="PKZ49_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HND37358.1"
                     /translation="GKHPQSVFSYKGKPITQVNTKAWYKALKRAGIKDFRWHDLRHTW
                     ASWLTQNGVPLNVIQEMGAWESAEMVRRYAHLAPEQFAQHARVLDGVLNGTNLAQSK"
     gene            complement(343..418)
                     /locus_tag="PKZ49_12180"
     tRNA            complement(343..418)
                     /locus_tag="PKZ49_12180"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(383..385),aa:Thr,seq:cgt)
     gene            complement(570..>1639)
                     /gene="priA"
                     /locus_tag="PKZ49_12185"
     CDS             complement(570..>1639)
                     /gene="priA"
                     /locus_tag="PKZ49_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HND37359.1"
                     /translation="LLTLRTRAIKNAALPVIQLIDTRSKLLQEGLSEPMLNALEKNLA
                     QNKQSMVFINRRGYAPILLCKSCAWMATCSRCSSRLVFHLQKQLLCCHYCGHQQPSPH
                     ACPHCGDPDLLPFGQGTQRIEAAIAKHFSSARIVRVDRDNISRKGAWAHIRQAIQAQQ
                     VDILIGTQLLAKGHDFPELSLVCILNADASLYSTDFRAEERLFSQLMQVAGRAGRAAI
                     VGTVLIQTEIPDHPLYAALIKHDYDHYAKKLLKERQTIDFPPYAYQALLNAEAPKLDS
                     ALNFLKNALTITPLAQSMQIYDPVPAQMPRLKGYERAHVLIQSHSRKKLQDFLANWYT
                     ELLKIKKTKVRWTIDVDPLEF"
BASE COUNT          451 a          347 c          381 g          460 t
ORIGIN      
        1 tggaaagcat cctcaatcag tatttagtta caagggcaag ccaatcacgc aagtgaatac
       61 caaggcttgg tataaggcgt taaaacgtgc cggtattaaa gattttcgct ggcatgactt
      121 acgccatacc tgggcaagct ggctgaccca aaacggtgtg ccgcttaatg tcatccaaga
      181 aatgggggca tgggaatctg ctgagatggt gagacggtat gctcacttag cacctgagca
      241 atttgctcaa catgctcggg tcttagatgg tgtgctcaat ggcacaaatt tggcacaatc
      301 gaagtaaaaa ggaatcagta gttacgctaa gttgttgatt tttggtgctg ttgagaggag
      361 tcgaacctcc gacctactga ttacgaatca gttgctctac caactgagct acaacagctt
      421 tgctgcatgc aagtgctgga attatagatg cttcatcaat taccagcaat gattcttcta
      481 tttaattcgc ttaacccact tcagaatgag ttgtgcaaaa taaattgagt tattccattt
      541 taggcaattc caaagatcaa actaaacccc taaaattcaa ggggatcaac atcgatagtc
      601 caacgcacct ttgttttttt tattttcaat agctcggtat accagttagc caagaaatct
      661 tggagttttt ttcgcgaatg cgattgaatc aacacatgcg cccgttcata gcctttgagc
      721 cttggcattt gtgcaggaac aggatcataa atttgcatag attgcgctaa aggcgtgata
      781 gtcaaagcat tctttaaaaa gtttagcgcg ctatctagtt taggcgcttc ggcattcaat
      841 agcgcctgat aagcataagg tgggaaatcg atagtttgtc tttctttgag taattttttt
      901 gcataatgat cgtaatcatg cttgattaaa gcagcgtaaa gcgggtgatc gggtatttcg
      961 gtttgaatga gtaccgtccc tactatggca gcccgccctg cgcgaccggc gacttgcatt
     1021 aactgtgaaa acaatcgttc ttccgctcgg aaatctgtgc tgtaaaggga agcatccgcg
     1081 tttaagatac acaccaacga taattcggga aaatcatggc ctttggcgag taattgggtt
     1141 ccgataagga tatcaacttg ctgcgcctgg attgcttggc gtatgtgtgc ccaagcacct
     1201 ttgcgactga tattatctcg atccacgcgt acaatcctgg cagatgagaa atgcttggca
     1261 atcgctgctt caatacgctg tgttccttga ccgaatggta gtaagtcagg atcaccacaa
     1321 tgtgggcaag catgtgggga tggttgctga tgaccacagt aatgacagca tagcaattgt
     1381 ttctgaagat gaaacaccaa ccgactcgaa caacgtgaac aagttgccat ccaggcgcag
     1441 gatttacaca gaaggatggg cgcataacct cggcgattga tgaacaccat actttgctta
     1501 ttctgcgcca gatttttttc gagcgcgttt agcattggct cagataaacc ttcttgaagc
     1561 aatttgctcc gggtatcaat caattgaatc acgggtaatg ctgcattctt gatcgctcgc
     1621 gttcgtagcg ttaataacg
//
LOCUS       DAOQKQ010000264         1532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_76331_length_1532_cov_4.483412, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000264 DAOQKQ010000000
VERSION     DAOQKQ010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76331_length_1532_cov_4.483412"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1201)
                     /locus_tag="PKZ49_12190"
     CDS             complement(<1..1201)
                     /locus_tag="PKZ49_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HND37360.1"
                     /translation="MSIRDRLQGIGSNLTATASQVAVTNPNHFTSYDNDAYHELKSRI
                     HQALLNRMDLAVMESLPQDRLLVEIKGLVERLLIEERTPINDIERLRIIQDIQHEVLG
                     LGPLEPLLADPTISDILVNTHRQVYVERRGKLELTDTQFANEAHLRKIIDRIVSRVGR
                     RVDESSPMVDARLPDGSRVNAIIPPLAIDGALLSIRRFAVIPLKIEDLIRNKSMTQEM
                     SEIISGLVKAKCSILISGGTGSGKTTLLNIMSAYIPHSERIVTIEDAAELQLQQPHVV
                     RLETRPPNVEGKGEVSQRALVRNSLRMRPDRVIIGEVRGAEAIDMLQAMNTGHEGSMA
                     TVHANTPRDALSRIENMVSMAGLNLPTKAVRHQISSAIVVVIQVSRLSDGKRKLINIQ
                     EITGMEGD"
     gene            complement(1257..>1532)
                     /locus_tag="PKZ49_12195"
     CDS             complement(1257..>1532)
                     /locus_tag="PKZ49_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator receiver protein"
                     /protein_id="HND37361.1"
                     /translation="GYPAEKIRLIVNRYDKKDEITLADVANTLKLEVFKAIPNDYNVV
                     AESVNQGIPVTKLAKRSAVAKSLHEFVQEMAQSSTSNKLLKKLFAFG"
BASE COUNT          407 a          382 c          300 g          443 t
ORIGIN      
        1 catcgccttc cattccagtt atttcttgaa tattgattaa cttacgtttt ccatccgaca
       61 atcgcgaaac ttgtatcacc acgacaatag ctgaactaat ttggtgccga accgctttgg
      121 taggtaagtt aaggccagcc atactgacca tattttcaat tcgactcaac gcatctcggg
      181 gggtattcgc atgaaccgtt gccatcgaac cttcgtgacc ggtattcatg gcttgcaaca
      241 tatcgatcgc ctcagcaccg cgtacctcac caataatgac gcgatcaggc cgcatccgta
      301 agctgtttcg aaccaaagca cgttgcgaaa cttctccttt accctcaaca ttgggaggtc
      361 ttgtttccaa ccgcacgaca tggggctgct gtaactgtaa ttcggctgcg tcttcgatgg
      421 taacaattcg ctcagaatgc ggaatataag cagacatgat attcagcagc gttgttttac
      481 cgctgccggt accgcctgaa atgagtatgc tgcacttcgc cttaaccaaa ccgctaataa
      541 tctcgctcat ttcttgggtc attgacttat tgcgaatcaa atcttctatc ttgagcggaa
      601 tcaccgcaaa acgccgaatc gataataaag caccatcaat cgcaagcggc ggtatgattg
      661 cattgacacg agaaccatcc ggcaatcggg catcaaccat cggactcgat tcatcgactc
      721 tacgcccaac tctagaaacg atccgatcga taatttttct taaatgtgcc tcgttggcaa
      781 attgggtatc ggtcagttcc agttttcctc gtcgttcaac ataaacttga cgatgggtat
      841 tgactaaaat atctgaaatc gtcgggtcag ccagtaaggg ctcaagggga cccaatccca
      901 gcacttcatg ctggatatcc tgaataatgc gtaatctctc gatatcattg attggtgttc
      961 gttcctcgat gaggagtcgt tccaccaacc ccttaatttc caccaacaat cgatcttgcg
     1021 gaagactttc catcactgcc aaatccatgc gattgagcaa tgcttgatgt atccgagact
     1081 tcaattcatg gtaagcatca ttatcataag aagtaaaatg attaggatta gtgaccgcaa
     1141 cctggcttgc agtcgctgtt aggttcgatc ctatcccttg aagacgatct cgaattgaca
     1201 taatcaatgt tccattaagt taaattttct tcctatcatc cacagcagca atgatgctaa
     1261 ccaaacgcaa ataatttttt cagcaactta ttgctggttg aactctgagc catttcttgg
     1321 acaaattcat gcagcgactt cgctactgca cttcgtttcg ctaatttagt caccggtatt
     1381 ccttgattga cagactcagc aacaacgtta tagtcattcg gtatggcttt aaagacttct
     1441 agctttaagg tatttgccac gtcagccagt gtaatctcat ctttcttgtc gtatcgattg
     1501 acaattaagc gtattttttc agcagggtaa cc
//
LOCUS       DAOQKQ010000265         1509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_78618_length_1509_cov_4.984869, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000265 DAOQKQ010000000
VERSION     DAOQKQ010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78618_length_1509_cov_4.984869"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="PKZ49_12200"
     CDS             <1..432
                     /locus_tag="PKZ49_12200"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HND37362.1"
                     /translation="RKHVVEVDQVEAMDWKHALKIGCAQCLALIPGTSRSGATIIGGL
                     FFGLSRKAAAEFSFFLAIPVMFAATFYDVYKNRDLLIIDDLTLFAVGFIAAFLSAWLA
                     IKGLMRYISNHDFTIFAWYRIVFGLVILTTAYSGVITWSSY"
     gene            complement(454..906)
                     /locus_tag="PKZ49_12205"
     CDS             complement(454..906)
                     /locus_tag="PKZ49_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HND37363.1"
                     /translation="MEDTTAILEKAHQRAEEMDLPYKGALTPAEALAILQTHPNAKLI
                     DVRCKAELDWVGRVPGAVEIELRTYPGMQPNPNFVDQVADRVDRNDLILFLCRSGGRS
                     DHAATIVAQSDFPNCYNILEGFEGDKDETGHRGTSGGWKAAGLPWVQS"
BASE COUNT          385 a          309 c          350 g          465 t
ORIGIN      
        1 agaaagcacg tcgtcgaggt tgatcaggtt gaagcgatgg actggaaaca cgcgcttaaa
       61 atcggttgtg cgcaatgctt ggcattaata ccaggaactt cgcggtccgg tgcgacgatt
      121 atcggggggc tattttttgg tttatcgcgc aaagcagccg ctgaattttc tttttttctg
      181 gctattcctg ttatgtttgc ggctactttt tacgatgtgt acaaaaatcg tgatttactg
      241 atcatcgatg atttaaccct gtttgcagtg ggttttattg ctgcattttt gagtgcatgg
      301 ctagccatca agggattaat gcgctatatc agtaaccacg acttcaccat ttttgcttgg
      361 tatcgcatcg tatttggcct ggttattttg acgacagcct attcgggcgt gattacttgg
      421 tcaagttact agcgcaataa acctatcaag aagttagctt tgcacccaag gtaatcctgc
      481 cgctttccag ccccctgatg ttccccgatg gccggtttca tctttatcgc cttcaaagcc
      541 ctctagaata ttgtaacaat tggggaaatc ggattgtgca acgatggttg cagcatgatc
      601 ggatcttccg ccactccggc acagaaatag gatgagatca tttcgatcga cgcgatctgc
      661 aacttgatca acaaaattag gattcggttg catgccggga taggttctaa gctcgatctc
      721 aaccgcccca ggaactctac cgacccaatc cagctcagct ttgcaacgga catcgatgag
      781 tttggcgtta gggtgagttt gtagtatcgc taaggcttct gcaggcgtta atgcgccttt
      841 gtaaggcaaa tccatttctt cagcgcgttg gtgcgctttt tctaatattg ctgttgtgtc
      901 ttccatttgt ttgtcacgtt aagggatatt agtaaattaa gccaataagt ttaattcaat
      961 gttagcgtag ttcacaaata ataatactga ctgaggaaac ggaggctata ttgaaacaat
     1021 taaccattgc tgtctgggtc ttcgttaggt aggctggcgc atcaaattag tgggactcga
     1081 tagattttct ggaagtcgtg ccatgttttt ggctttatcg ttgttctatc ttcagaaagt
     1141 attggagttg ttcatagaca atcaggatct atcgagtcta gcgctttcga ttctcgcgtt
     1201 tgactagata tccctttggt tggtgggaag gtggaatcgt aagctcatca aaatttctga
     1261 tctgaaacta acgtaatgac ttacaattcg atagtatgat caagagagta aaattaataa
     1321 actggagtta gtaaatgaga aaattaaata ttgctttaac cctatcagca ttattcgttc
     1381 tgactgcttg tggcgggagc aaagactatt caccgccagc cgatgcatct ggagaacaaa
     1441 ttttcagtac ggcctgcacg gaatgccata aacccctcag cgcaaacgtt gccatgatat
     1501 taagcgaga
//
LOCUS       DAOQKQ010000266         1506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0570
            NODE_78912_length_1506_cov_4.649897, whole genome shotgun sequence.
ACCESSION   DAOQKQ010000266 DAOQKQ010000000
VERSION     DAOQKQ010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810202
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:46:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,444
            CDSs (total)                      :: 2,413
            Genes (coding)                    :: 2,399
            CDSs (with protein)               :: 2,399
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 5 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78912_length_1506_cov_4.649897"
                     /isolate="AMR_MDS_0570"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PKZ49_12210"
     CDS             <1..253
                     /locus_tag="PKZ49_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HND37364.1"
                     /translation="YLCTQCKLQFETRHSINATNPDCPVCGSTTEKVILSAPAAHGNM
                     ARGRDLAIRSLQPKTDPVNHLHVPGCGCSRHKHLGYVKK"
     gene            complement(675..974)
                     /locus_tag="PKZ49_12215"
     CDS             complement(675..974)
                     /locus_tag="PKZ49_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HND37365.1"
                     /translation="MSAVVWLDEALEDLKSIGDYIAQENANAAYNVLIRIKATANNLS
                     LHPEIGRPGRVFGTREIVMSDLPYILAYQITDRDIRILAVMHTSRKWPENFNRLR"
     gene            complement(971..1219)
                     /locus_tag="PKZ49_12220"
     CDS             complement(971..1219)
                     /locus_tag="PKZ49_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HND37366.1"
                     /translation="MASETGRMSVSVAPEKQEKLDAIAHSLDRSRNWLVNQAIDHYLE
                     IYDWQTKKIQERLDIASGKNAKFHSSDDVDAMIDKFKA"
BASE COUNT          429 a          305 c          299 g          473 t
ORIGIN      
        1 ttacttgtgc acacagtgca aactacaatt cgagacacgc cattcaatca atgcgaccaa
       61 tccagattgc ccggtatgcg ggagtacgac agaaaaggtt attctttctg ctcctgcggc
      121 gcatggcaac atggcgcgtg gccgtgatct ggcaatacgt tcacttcaac caaaaaccga
      181 tccggtaaat cacctacacg tgcctgggtg cggatgtagt cgtcataagc atttaggata
      241 tgttaagaaa taaccactac tgtctcgtta actttcacag tagagtcaac tgaatatcag
      301 acgcgtgatt atggaatggc ggatcatctc gcaatgtgac ctgacgcccc aacggtttaa
      361 tcatcggggt tttctataca ctttgttcgc cgaattttcg agctcaatgg ttaatccatc
      421 acgctataaa gcgatcagaa attcacaaaa tttgtgttta atcgacaaca aatagcgtta
      481 attctggatc ttttttaaag gataaaaagg ttattaatgc agtgtcattt tgatgtcaat
      541 ggacacacta ctccaatttg aatcatttgc agtatggcat gacgagctgt aatagaggtc
      601 tttcatgttg taatggaatg gatgttcgct atttttacga tatcgaagtg ccaaagttgt
      661 tgtaagcata accactagcg taatcgattg aaattttcag gccattttct ggatgtatgc
      721 atgacagcta atatgcgaat atctctgtcg gttatttgat aggcaagaat gtatggaaga
      781 tcgctcatca ctatttcacg cgtaccaaac acacggcctg ggcgaccaat ttcggggtgg
      841 agtgatagat tattggcagt agcttttatt ctaattaaaa cattataagc agcattggcg
      901 ttctcttgtg caatataatc gccaattgat tttaaatctt ctagtgcttc atcgagccat
      961 acaacagcgc tcatgctttg aatttatcta tcatggcatc aacatcatcg cttgaatgaa
     1021 attttgcatt tttccctgaa gcaatatcca gacgttcttg aatttttttt gtttgccaat
     1081 cgtaaatttc cagatagtga tcaatagcct gattaactaa ccagtttctg gaacgatcca
     1141 gactgtgcgc aattgcatcc agcttttctt gtttttctgg tgccactgat acactcatgc
     1201 gtcctgtttc gcttgccatt tcttattcct taatcagaag taatcatgtt taatcttggc
     1261 atagataagt atgatttgca aattgaaaca gcaaacagat attgaaacaa tcattgctaa
     1321 gaaatgactg tgtcaaattt gtgccaaact gcggcagaaa taccatcatt tgaggctgtt
     1381 tgacactggt tttgtgcttg gcaagcattg taagcgattg attatattaa ttgcatattc
     1441 aatggctgcg tctcataatc ccttggtcgt aggttcaagt cctaccagac ccaccataaa
     1501 attaag
//
